Miracle-dz/UniFAR-FTUs huggingface dataset:
Isolation and characteristics research of L-sorbose dehydrogenase in Ketogulonigenium sp.WB0104

Ketogulonigenium sp.WB0104 is the second microorganism in the production of 2-keto-L-glulonate(2-KLGA),which is an important procursor of Vintim C.L-Sorbose Dehydrogenase(SDH) was isolated from the cell free extract of the culture of WB0104 by centrifugation,ultrasonication,ultracentrifugation and chromatography on SP-Sepharose Fast Flow,DEAE-CL6B and gel filter.The molecular weight(MW)of SDH was 60.499 kd by mass-spectrum(MS) analysis and was 139.053±4.96kd by gel filter anylasis of superdex 200,which showed that SDH was a dimer incluing identical subunits.Prosthetic group was identified as pyrroloquinoline quinone(PQQ). SDH activity was assayed according to the method of 2,6-dichlorophenolindophenol as electron acceptor and L-sorbose as a substrate.Km value was 23.94 mmol/L by the analysis method above mentioned with L-sorbose as substrate. In vitro bioactivity anaylsis showed SDH could convert L-sorbose to 2-KLGA directly under the condition of phenazine methosulfate(PMS) as electron acceptor.
Response of Soil Microbial Communities to Warming and Clipping in Alpine Meadows in Northern Tibet

In order to explore responses of soil microbial communities among different alpine meadows under warming and clipping, soil microorganisms of three alpine meadow sites (low altitude: 4313 m, alpine steppe meadow, 30°30′ N, 91°04′ E; mid-altitude: 4513 m, alpine steppe meadow, 30°31′ N, 91°04′ E; and high altitude: 4693, alpine Kobresia meadow, 30°32′ N, 91°03′ E) were measured using the phospholipid fatty acid (PLFA) method. Both warming and clipping significantly reduced PLFA content and changed the community composition of soil microbial taxa, which belong to bacterial and fungal communities in the alpine Kobresia meadow. Warming significantly reduced the soil total PLFA content by 36.1% and the content of soil fungi by 37.0%; the clipping significantly reduced the soil total PLFA content by 57.4%, the content of soil fungi by 49.9%, and the content of soil bacteria by 60.5% in the alpine Kobresia meadow. Only clipping changed the total fungal community composition at a low altitude. Neither clipping nor warming changed the microbial community composition at a moderate altitude. Soil temperature, soil moisture, and pH were the main factors affecting soil microbial communities. Therefore, the effects of warming and clipping on soil microbial communities in alpine meadows were related to grassland types and soil environmental conditions.
Forest Trees of Australia

Forest Trees of Australia is the essential reference for observing, identifying and obtaining information on the native trees in this country. It describes and illustrates over 300 of our most important indigenous trees, which have been carefully selected for their environmental significance, their importance to the timber industry, or their prominence in our landscape. This new and thoroughly revised edition has been fully updated throughout and includes treatments of 72 additional species. New maps and photographs show us a wonderfully diverse range of forests, from mangrove swamps, tropical regions and deserts, to alpine areas and majestic stands of temperate forests. A colour section illustrates some of the major forest types of Australia and bark from a diverse range of species. Forest Trees of Australia is an unsurpassed guide to identification for horticulturists, botanists, foresters, students, farmers, environmentalists and all those who are interested in our native trees.
Etude comparative de l’effet de l’antidépresseur sur la symptomatologie dépressive chez une population de schizophrènes avec troubles dépressifs en comorbidité

Comparative study of antidepressant effect on depressive symptomatology among two groups of a population of schizophrenia with depressive disorders in co-morbidity followed at the level of the Department of Psychiatry of the Tlemcen CHU 2017-2018”. Introduction: The objective of our work is to compare the effect of the antidepressant on depressive symptomatology among a population of schizophrenia with depressive disorders in co-morbidity treated by an antidepressant and a population of schizophrenia and depressive Psychiatry of the CHU Tlemcen for a period of one year. Method: This is a comparative study carried out on a sample of 207 patients followed at the level of our psychiatric consultation. Our sample was split into two groups, those disorder depressive meds antidepressant and those with depressive in co-morbidity disorder received no antidepressant treatment. A comparison between the two groups was conducted; a description of the characteristics of sociodemographic, clinical, a comparison of the quality of life depressive symptomatology (SQoL scale) and PANNS scale between the two groups. Results: In our study antidepressants are effective on the symptomatology of depressive with a net action on the negative dimension of schizophrenia as well as an improvement in the quality of life scores. Efficiency demonstrated in the treatment of co-morbidity disorder depression and schizophrenia for tricyclics and SSRIS. The duration during which the treatment should be continued is not defined, but it seems that a stop too early, before six months, promotes relapses. Quality of life is better in patients with disorders depressive in comorbidity on treatment anti depressant, as well as patients on SSRIS compared to tricyclics with scores in the SQoL (overall score) of 68 for the first sub-group SSRIS and 64 for the second under tricyclic group. Most affected was the sentimental life, the self-esteem, psychological well-being. Conclusion: Many arguments which prompt us to search systematically for the comorbidity and put in place a support appropriate, which would probably reduce the severity of schizophrenic disorder, the frequency of hospitalizations, and improve the quality of life of these patients and therefore a better social integration.
Galactosidases in tomato fruit ontogeny: decreased galactosidase activities in antisense ACC synthase fruit during ripening and reversal with exogenous ethylene

α- and β-galactosidase (α- and β-Gal) activities, firmness and pigment content were analysed in tomato (Lycopersicon esculentum Mill.) pericarp during fruit growth and ripening, comparing normal fruit with transgenic fruit containing an ACC-synthase antisense transgene. Normal and transgenic immature green fruit had similar temporal patterns of total α- and β-Gal activity. Immature 21-day-old fruit displayed 93% and 134% higher α- and β-Gal activity on a per gram fresh weight basis, respectively, than mature-green fruit. During ripening, normal fruit presented increasing levels of α- and β-Gal activity towards the red-ripe stage. β-Gal II was detected in mature-green tomatoes; it rose rapidly and reached maximum values at the red-ripe stage. In contrast, α- and β-Gal activity in antisense fruit decreased after reaching the breaker stage, and a low continuous level of activity was apparent between 54 and 108 days after anthesis. 48- to 108-day-old transgenic fruit showed constant basal levels of β-Gal II. There were no significant differences in enzyme activity between antisense attached and detached fruit. An exogenous ethylene treatment performed in transgenic tomatoes brought about a promotive effect on total α- and β-Gal activity in general and on β-Gal II in particular, thus suggesting a role for ethylene in de novo synthesis or activation of these enzymes. Softening, chlorophyll breakdown and lycopene biosynthesis were impaired in the antisense fruits, but the impairment was only complete for lycopene synthesis and all were reversed by applied ethylene. These results can be associated with the signal transduction pathways proposed to be operational during tomato ripening.
The Effect of Precursor Feeding on Flavonoids Biosynthesis in Cell Suspension Cultures of Glycyrrhiza inflata Bat.

The effects of four precursors on flavonoids biosynthesis in cell suspension cultures of Glycyrrhiza inflata Bat.were studied.The results showed that all of these precursors with the appropriate concentrations not only affected the cell growth,but also promoted the flavonoids biosynthesis.Moreover,the flavonoids productions were all beyond 100 mg/L when the best addition concentrations of phenylalanine,tyrosine,cinnamic acid and NaOAc were 20,5,5 and 5 mg/L,respectively.The optimum time for tyrosine addition was the 5th day during the cell culture,while it was the 10th day for the other three kinds of precursors.Among the four precursors,tyrosine was the most effective in which the maximum flavonoids production reached 113.0 mg/L,1.43 times as that of the control.Additionally,the addition of phenylalanine and NaOAc enhanced the activity of phenylalanine ammonialyase,one of the key enzymes of the flavonoids biosynthesis,while the addition of cinnamic acid deduced it.
Morphological drivers of trophic cascades

Worldwide, local anthropogenic extinctions have recently been reported to induce trophic cascades, defined as perturbations of top consumers that propagate along food chains down to primary producers. This focus on the effects of top-consumer extinction (i.e. of species presence) ignores potential cascading effects of the rapid morphological changes that may precede extinction. Here, we show in an experimental, three-level food chain including medaka fish, herbivorous zooplankton and unicellular algae that varying body length of a single fish from large (36.3 mm) to small (11.5 mm) induced a stronger trophic cascade than varying an average-sized (23.8 mm) fish from being present to absent. The strength of fish predation on zooplankton scaled quasi linearly (not with a power exponent) with fish body length and associated gape width, suggesting that the resultant trophic cascade was morphology (not metabolism)-dependent. The effect of fish body length was stronger on phyto- than on zooplankton, because large-sized fish had the unique ability to suppress large-sized herbivores, which in turn had high grazing capacities. Hence, our results show that consumer body size, by setting diet breadth, can both drive and magnify the strength of trophic cascades. In contrast, fish body shape had no significant effect on fish predatory performances when its allometric component (the effect of size on shape) was removed. In the wild, human-induced body downsizing of top consumers is widespread, and mitigating the resultant perturbations to ecosystem function and services will require a paradigm shift from preserving species presence towards preserving species size structure.
Microvascular-based Hierarchical System for Sensing-Healing of Delamination in Composite Structures

ID No : 233 ABSTRACT Our previous study proposed a hierarchical sensing-healing system combining a microvascular self-healing material and our fluid distribution system with local pressure monitoring. The comboned system could increase efficiency and reliability of the healing. In this study, the sensing/healing performance was evaluated using a compression after impact (CAI) test of a composite stiffened panel. Even though further optimization of the healing resin and the microvascular network is necessary, the hierarchical system restored the structural stability and recovered the degraded strength, confirming its high potential under practical conditions.Our previous study proposed a hierarchical sensing-healing system combining a microvascular self-healing material and our fluid distribution system with local pressure monitoring. The comboned system could increase efficiency and reliability of the healing. In this study, the sensing/healing performance was evaluated using a compression after impact (CAI) test of a composite stiffened panel. Even though further optimization of the healing resin and the microvascular network is necessary, the hierarchical system restored the structural stability and recovered the degraded strength, confirming its high potential under practical conditions.
TAILORING THE INTERFACIAL INTERACTIONS IN FERROELECTRIC FLUORINATED POLYMER/CERAMIC NANOCOMPOSITES

In this work composites of PVDF-TrFE containing 60 vol% untreated and surface modified BaTiO3 were produced by solvent casting with two procedures. Their morphology and structure were characterized by scanning electron microscope, X-ray diffraction and differential scanning calorimetry. The effect of the processing conditions and of the surface modification of BaTiO3 on the viscoelastic, dielectric and piezoelectric properties was investigated. The surface modification of BaTiO3 allowed obtaining composite films with low porosity and good filler dispersion, and hence higher storage modulus and lower loss tangent, in a wider processing window. Furthermore it reduced the dielectric losses at low frequency and modified the decay kinetic of the d33 piezoelectric coefficient with respect to composites made with untreated particles.
Impact of Cardiac Resynchronization Therapy on Indirect Inflammatory Markers

Background: Cardiac resynchronization therapy (CRT) is an established treatment for patients with symptomatic chronic heart failure with reduced ejection fraction (HFrEF) and prolonged QRS despite optimal pharmacological therapy. Inflammation plays a crucial role in the pathogenesis and progression of cardiovascular disease. The role of CRT pre-implantation inflammatory condition assessed using routine laboratory tests has been rarely investigated. In this study we aimed to evaluate the effect of CRT on indirect inflammatory markers such as neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and lymphocyte-tothe monocyte (LMR) ratio. Methods: 75 CRT patients were included in the study retrospectively. Before the CRT implantation, clinical and demographic data were recorded from all patients. NLR, PLR and LMR ratio were measured before CRT implantation. The patients were reevaluated minimum six months after CRT; the above-mentioned parameters were measured again and compared to the pre-CRT period. Results: Compared to the period before CRT, laboratory findings such as white blood cell (3.5 ± 2.2 103 uL vs. 3.2 ± 2.4 103 uL; p = 0.006), neutrophyl (1.9 ± 0.4 103 uL; vs. 1.4 ± 0.4 103 uL; p = 0.002), NLR (3.8 ± 0.3 103 uL; vs. 1.7 ± 0.1 103 uL; p <0.001), PLR (490.2 ± 199 103 uL; vs. 381 ± 105 103 uL; p < 0.001) levels were significantly lower after 6 months of CRT implantation. Lymphocyte counts (0.5 ± 0.3 103 uL vs. 0.8 ± 0.2 103 uL; p = 0.001) were significantly higher in the post CRT group. A significant and positive correlation of the reduction in NLR (rs = 0.362, p = 0.001) and PLR (rs = 0.562, p <0.001) was found with the increased six minute walking test (6-MWT). Conclusion: The NLR, PLR and MLR were decreased after CRT implantation. The modest decrease in these parameters demonstrates the effect of restoring the heart’s electromechanical synchrony after CRT on inflammation.
Scale-Up of Self-Regenerating Semi-Batch Adsorption Cycles through Concurrent Adsorption and Reduction of Cr(VI) on Sheep Wool

A previous publication by our group reported that adsorption of Cr(VI) on sheep wool reached 99% when allowed a long residence time, with concurrent reduction to Cr(III). In this study, the process was scaled up by optimizing a pilot plant based on semi-batch adsorption cycles. This yielded Cr(III), which is about 300 times less toxic than Cr(VI), and can be precipitated using lime at high pH. Since the reduction step is slower than the adsorption one, an adsorption column was designed to perform semi-batch operation cycles, whereby the extended “off cycle” allows reduction to take place. Since reduction of Cr(VI) frees active sites on wool, the plant acts in lieu of in situ regeneration, accompanied by additional adsorption of Cr(VI). The results show that 97% of the column efficiency can be recovered within 24 h of “off cycle”. Wastewater from a local electroplating industry was treated by this method with high removal of Cr(VI), reaching the limit permitted by environmental standards. This study also reveals that typical concentrations of heavy metals, present in wastewater produced from electroplating, had no substantial antagonistic interference with Cr(VI) adsorption.
Efficacy Trials of Different Types of Biological Insecticide against Prodenia Litura(Fabrwius)

The efficacy trials of several types of biological insecticides against 3th instar larvae of P.litura were carried out in laboratory by using flooding method.The results of insecticide application showed that the insect death rates of Beauveria bassiana(dilution 1∶ 250) and polyhedrosis virus(dilution 1∶ 250) were up to 100 % after 1 day application,their control effects were better than that of avermectin(dilution 1∶ 5000) and apinosad(dilution 1∶ 5000),which the adjusted death rates reached over 90 % after 3 days and at 100 % after 4 days application.However,the remaining treatments of meliae toosendan(dilution 1∶ 100),matine(dilution 1∶ 600) and Bacillus thuringiensis(dilution 1∶ 250) performed worst,which had low adjusted death rates at 67.2 %,59.0 % and 66.7 % respectively after 8 days application.Overall,the control efficacy in lab of Beauveria bassiana,polyhedrosis virus,avermectin and apinosad was superior to usually used chemical insecticide,cypermethrin.
siRNA-induced caveolin-1 knockdown in mice increases lung vascular permeability via the junctional pathway

: Caveolin-1, the principal integral membrane protein of caveolae, has been implicated in regulating the structural integrity of caveolae, vesicular trafficking, and signal transduction. Although the functions of caveolin-1 are beginning to be explored in caveolin-1 -/- mice, these results are confounded by unknown compensatory mechanisms and the development of pulmonary hypertension, cardiomyopathy, and lung fibrosis. To address the role of caveolin-1 in regulating lung vascular permeability, in the present study we used small interfering RNA (siRNA) to knock-down caveolin-1 expression in mouse lung endothelia in vivo . siRNA caveolin-1 the expression caveolin-1 wild-type the concomitant disappearance of caveolae in lung vessel endothelia and dilated inter-endothelial junctions well as increased lung vascular permeability to albumin via IEJs. reversal of caveolin-1 coupled to restoration of
Effectiveness of Methylene Blue in the Prevention of Stifle Joint Arthrofibrosis in Rabbit Models.

Background One of the major challenges in orthopedic surgery is the prevention of arthrofibrosis, which can be successfully alleviated in its early stages. Many studies suggest the administration of methylene blue (MB) as an accessible and effective agent for the prevention of post-operation adhesions. The purpose of this study was to evaluate the efficacy of MB in the prevention of arthrofibrosis. Methods This study was conducted on 18 New Zealand white female mature rabbits. The anterior cruciate ligament of the left stifle joint of each animal was cut during aseptic surgery. In the next step, the rabbits were divided into three groups based on their treatments. The rabbits in the first, second, and third groups were subjected to the injection of normal saline, 1% MB solution, and 2% MB solution into their synovial space, respectively. The postoperative stifle range of motion was measured every week. After 4 weeks, the animals were euthanized and all joints were dissected for histopathology. Results The histopathological evaluation of tissues indicated the presence of fibrotic connective tissue as a sign of fibrosis in all groups. The fibrosis rate, inflammatory rate, tissue disarrangement, fibroblastic cellularity, and granulation tissue formation were at their highest levels in the 1% MB group. The integrity of articular cartilage in the 2% MB group was lower than the other groups. The level of bone degeneration was similar in both MB groups; however, it was more than the control saline group. The range of motion was reduced during the first week, then increased in the second and third weeks, and finally decreased in the fourth week. Conclusion The MB was not an effective method regarding the prevention or treatment of arthofibrosis and the subsequent osteoarthritis. In contrast with previous studies, fibrosis was lower in the high dose MB group, compared to the low dose MB group. The alterations in the range of motion were related to the reduced movement caused by the pain and stiffness of the operated joints. The current study can be considered as the first report addressing the adverse effect of MB on synovial components. Level of evidence I.
New eudesmane sesquiterpenoids from Eremophila scoparia

Three new eudesmane sesquiterpenoids have been isolated from Eremophila scoparia. They are (4aR,6S,7S,8aS)-7-hydroxy-6-(1-hydroxy-1-methylethyl)-4,8a-dimethyl-4a,5,6,7,8,8a-hexahydronaphthalen-2(1H)-one, (4aS,6S,7S,8aS)-7-hydroxy - 6-(1 -hydroxy -1-methylethyl)-4,8a-dimethyl-4a,5,6,7,8,8a-hexahydronaphthalen-2(1H)-one and (2S,3S,4aR,8aS)-3-(1-hydroxy-1-methylethyl)-5,8a-dimethyl-7-oxo-1,2,3,4,4a,7,8,8a-octahydronaphthalen-2-yl acetate.
Effect of Catechin and Epicatechin on Serum Lipid Level in Mice

This article studies the effects of the same catechin by different administrations and of the different catechins by the same administration on serum lipid level in typerlipidemic mice.The results show that catechin and epicatechin by using intravenous injection or peros significantly decrease TC,TG,LDL-C and increase HDL-C in hyperlipidemic mice.The effect of catechin is better than that of epicatechin.
Neo-Mitchellian Approach to Understanding Stakeholders Relationships in Organizations

Mitchell et al. (1997) proposed that stakeholder salience is directly proportional to the number of attributes of salience—power, legitimacy and urgency—as perceived by managers. However, till date, researchers have differences about these attributes as determinants of salience. To resolve these differences and to precisely specify the attributes that make a stakeholder salient in the eyes of managers we attempted to gain deeper insights into this phenomenon. For this purpose, we employed multiple case study strategy in a purely quantitative treatment—a unique mix. Our findings suggest that the attributes as conceived by Mitchell et al. (1997) are quite broad and general. Managers identify salient stakeholders based on specific types of these and few other attributes. We found that stakeholders that possess utilitarian power, influence legitimacy, criticality and organized proximity are attended by the managers.
Mutagénesis ambiental y el uso de biomarcadores para predecir el riesgo de cáncer

Environmental mutagenesis and use of biomarkers in cancer risk prediction. The field of envi- ronmental mutagenesis or toxicology genetics aims to study the genetic damage that leads to mutations produced by physical, chemical and biological agents, to identify these agents and analyze their interactions and ways of action. There are enough experimental and epidemiological evidences implicating mutations in oncogenes, tumor suppressor genes and DNA repair genes as determinants in the onset and progression of the neoplastic process. A valuable tool in public and occupational health is the monitoring of populations exposed to poten- tially hazardous agents. The objective is to protect the health and quality of life of high risk groups on account of the nature of the agents of exposure. Monitoring of genotoxic effects in exposed populations as well as the analysis of susceptibility polymorphism are visualized as key tools in the realm of future public and occupational health in order to prevent the occurrence of environmental and specially occupational origin of tumors. This paper reviews the main concepts concerning this issue and refers to studies on the subject in Costa Rica. Rev. Biol. Trop. 52(3): 585-590. Epub 2004 Dic 15.