Miracle-dz/UniFAR-FTUs huggingface dataset:
Development and Application of a PCR-Based Molecular Marker for the Identification of High Temperature Tolerant Cabbage (Brassica oleracea var. capitata) Genotypes

Global warming accelerates the development of high temperature (HT)- and high humidity (HH)-tolerant varieties. This is further facilitated by the identification of HTHH-tolerant genes and the development of molecular markers based on these genes. To identify genes involved in HTHH tolerance in cabbage (Brassica oleracea var. capitata), we performed RNA-seq analysis of two inbred lines, BN1 (HTHH-tolerant) and BN2 (HTHH-susceptible), and selected trehalose 6- phosphate phosphatase I-2 (BoTPPI-2) as one of the HTHH-tolerant-associated genes. We also developed a segregating F2 population from a cross between BN1 and BN2. RNA-seq results showed that BoTPPI-2 transcript levels were high in the HTHH-tolerant inbred line BN1, but not detectable in the HTHH-susceptible inbred line BN2. The expression pattern of BoTPPI-2 was not related to the expression of heat shock-related genes. Soft rot resistance, used as an indicator of HTHH tolerance, was higher in BN1 than in BN2. F2 individuals similar to BN1 with respect to phenotype appeared to be HTHH-tolerant, whereas BN2-types were susceptible to HTHH. Analysis of the genomic DNA revealed the presence of a long terminal repeat (LTR; ca. 4.6 kb) in the ninth intron of the BoTPPI-2_BN2 allele, thereby suppressing its transcription and exhibiting HTHH phenotype. Except for the LTR insertion, the sequence of BoTPPI-2_BN2 was almost identical to that of BoTPPI-2_BN1. On the basis of the LTR and BoTPPI-2 sequences, we developed a molecular marker to identify HTHH-tolerant genotypes and validated its efficiency using F2 individuals, inbred lines, and cultivars from diverse sources. The marker explained the genetic basis of HTHH tolerance in at least 80%, but not 100%, of the cabbage genotypes. Thus, additional markers associated with HTHH tolerance are needed for perfect selection.
Domain specificity of motivation: chemistry and physics learning among undergraduate students of three academic majors

ABSTRACT This study aimed at investigating the issue of domain specificity of motivation across specific science disciplines (chemistry and physics) in combination with influencing factors related with students’ academic major and gender. A clustered sampling was used to select 281 participants who were undergraduate students (105 males and 176 females) attending three different academic majors (Environmental Technology, Conservation of Cultural Heritage, Food Technology) at a Greek tertiary education institution. Motivation to learn chemistry and physics was evaluated concurrently using the discipline-specific (Chemistry and Physics) versions of Science Motivation Questionnaire II (SMQ II) denoted as CMQ II and PMQ II, respectively. Discipline-based comparisons showed the existence of domain specificity in motivation with the whole student sample displaying higher motivation to learn chemistry relative to physics in all five motivational scales of SMQ II (self-efficacy, self-determination, intrinsic motivation, career motivation and grade motivation). Multivariate analysis of variance (MANOVA) and discriminant function analysis showed that students’ motivation to learn chemistry and physics depends strongly on their academic major. Furthermore, MANOVA provided evidence for a small gender effect on motivation for either chemistry or physics learning. The implications of the current study for effective tailor-made educational practice and research across different science disciplines are critically discussed.
Ege Üniversitesi Hastanesinde glioblastomaların epidemiyolojik ve sağ kalım özellikleri

Amac: Ege Universitesi Hastanesi (EUH) veri tabanindaki 1327 Glioblastoma (GBM) olgusunun epidemiyolojik ve genel sag kalim (GSK) ozelliklerini istatistiksel acidan degerlendirmektir. Gerec Yontem: EU Kanserle Savas Uygulama ve Arastirma Merkezi (EUKAM) tarafindan 1992-2017 arasinda kanser veri tabaninda toplanan 117139 kanser verisi icindeki 1327 GBM olgusu degerlendirilmistir. Bulgular: 1327 GBM hastasi analiz edilmistir. Tani ani ortalama yas 55,6 yil bulunmustur. Erkek/kadin orani 1,52:1’dir. En sik tutulumun temporal lobta (%36,3) oldugu saptanmistir. Tedavisi raporlanan 881 hastanin analizinde; olgularin %30,2’sine yalnizca operasyon, %65,6’sina kombine tedaviler uygulanmistir. Otuz bes hastada (%4,0) yalnizca radyoterapi (RT) uygulanirken 558 hastada (%63,3) kombine tedavide RT eklenmistir. Tum GBM hastalarinin ortalama sag kalim suresi 12,15 aydir. Bir, iki, uc ve bes yillik GSK oranlari sirasiyla %50,4, %22,02, %11 ve %5’tir. Ortalama sag kalim erkeklerde 11,6 ay, kadinlarda 13,3 ay olarak saptanmistir. Cinsiyet ile sag kalim suresi arasinda anlamli iliski saptanmamistir. Yas gruplarina gore sag kalim analizinde ileri yas gruplarinda sag kalimin anlamli olarak azaldigi saptanmistir (p<0,001). Cerrahi, RT ve kemoterapi (KT) uygulanan hastalarin bir ve bes yillik GSK oraninin diger tedavi sekillerine gore daha yuksek oldugu saptanmistir (p<0,001). Ortalama sag kalim RT alan grupta 14,0 ay, almayanlarda ise 9,4 ay olarak bulunmustur (p<0,001). Sonuclar: Calismamizda tani yasi, eszamanli ve adjuvan KT (Temozolomid) tedavisi sag kalimi etkileyen prognostik faktorler olarak bulunmustur. Cinsiyet ve tumor lokalizasyonu ile sag kalim arasinda anlamli fark saptanmamistir. Gunumuzde maksimum cerrahi rezeksiyon ve ardindan adjuvan kemoradyoterapi, GBM yonetiminin temel standardi olup, ortalama 12 aylik GSK saglamaktadir.
Grasp : Design and developement of a 6DOF, 3D printable, open source bionic hand

There are 3 millions of upper limb amputees worldwide, and this represents a minority of 5% of the total number of amputees. For this reason, the costs of production of an advanced bionic limb are so elevated. This impacts on the acquisition prices, setting them between 25.000 and 75.000 €. The main motivation of the present work has been to create a design of a bionic hand with 6 degrees of freedom (DOF), which can be 3D printed with competitive features respect to the best commercial models available in the market in terms of flexion-extension speed and force. Each of the aims of the project has been a different technological challenge. The main objectives can be summarised as follows: (1) the bionic hand must be able to reproduce the 6 most important types of grips required in order to carry out activities of daily life; (2) all the components: electronics and actuators should be included into the palm of the hand to reduce the possible space occupied in the forearm; (3) the hand mass should be less than 400g, which the weight critical for the user to be considered too heavy; (4) the bionic hand should be easily assembled and programmed by the same user once you have downloaded the open source files; (5) the total cost of the hand must be below 500 € including all components. Finally, the greatest challenge is to achieve all these objectives and at the same time be competitive with respect to existing commercial projects. The project includes the development of various designs and 3D printable prototypes that have been carried out during the previous 4 years. All the experience and knowledge acquired with these prior designs culminated in Grasp: the latest design, presented in this work. Grasp sets out to achieve all the objectives that have emerged from the needs that must be covered to provide a product with the optimal quality and functionality.
AIDS prevention among adolescents: an intervention study in northeast Thailand.

Both qualitative and quantitative methodologies (including a baseline survey focus group discussions a 6-month intervention program a follow-up survey and a final set of focus group discussions) were used to evaluate the impact of a school-based peer counseling program in Northeast Thailand aimed at increasing acquired immunodeficiency syndrome (AIDS) awareness. 2909 students at 2 experimental and 2 control schools participated in the evaluation. The pre-invention focus group discussions revealed in females a conflict between a personal desire to maintain their virginity and social pressure to become sexually active and among boys widespread experience with prostitutes and a reluctance to use condoms with girlfriends. In the experimental group peer counselors were provided with a mobile AIDS exhibition unit audiovisual materials and guidelines for an AIDS drama competition. Peer counselors were trained to conduct both individual and group sessions. At follow-up the level of AIDS awareness (high at baseline in both groups of schools) showed no significant increase in either group; knowledge regarding condom use increased in both the experimental and control settings. This finding may reflect the simultaneous introduction by the National AIDS Prevention Program of AIDS educational programs in all schools in Thailand. However follow-up focus group discussions indicated that students in the experimental schools had greater access to condoms were more comfortable discussing sexual behaviors with the peer counselors than friends and considered themselves to have more behavioral options to prevent AIDS than controls. Overall students tended to utilize peer counselors as a source of advice and service referral. Within their schools peer counselors were viewed as role models of health protective behaviors. Thus qualitative program evaluation provided important feedback that was not accessible through quantitative analysis.
Identifying historical baseline at the onset of exploitation to improve understanding of fishing impacts

Marine communities have long been impacted by human activities, but the quantification of human‐driven changes often relies on recent data. This is because historical data on fish populations are lacking and are challenging to include in contemporary stock and ecological assessments. As a result, the impacts of early fishing pressure on marine communities are generally poorly documented worldwide. Marine communities of Southeast Australia have a relatively short history of exploitation compared with other temperate systems and were sampled before and after the onset of commercial fishing. As such, they provide a rare opportunity to identify historical baselines and to understand ecological changes after the onset of commercial exploitation. This study compares survey data collected around Tasmania, Southeast Australia, in 1909–1910 with data from the 1980s. The period considered precedes the establishment of a trawl fishery in Southeast Australia in 1915, of other important commercial fisheries in Tasmania, and of a fisheries data collection programme in 1984. Nominal catch rates are used to examine changes across all families of demersal fish recorded in catches and generalized linear models are used to estimate and compare standardized indices of abundance between the 1909–1910 and 1980s data for key commercial families. Results show significant, and thus far unreported, fishing‐induced changes in marine communities after the establishment of commercial fishing in the region. Changes mostly relate to shifts in catch composition and steep declines in the abundance of the main commercial families. This study illustrates a method for analysing low‐quality historical catch data and provides estimates of pre‐commercial fishing abundance that can be included in stock and ecological assessments. More broadly, this study demonstrates the significant role of early fishing in shaping marine communities and increases our understanding about general patterns of exploitation that have been difficult to identify with longer but less detailed fishing histories.
Somatic Mutations in Cancer-Free Individuals: A Liquid

Somatic mutations have been perceived as the causal event in the origin of the vast majority of cancers. Advanced massively parallel, high-throughput DNA sequencing have enabled the comprehensive characterization of somatic mutations in a large number of tumor samples for precision and personalized therapy. Understanding how these observed genetic alterations give rise to specific cancer phenotypes represents an ultimate goal of cancer genomics. However, somatic mutations are also commonly found in healthy individuals, which interfere with the effectiveness for cancer diagnostics.
Misallocation and risk sharing

This paper shows that factor misallocation is closely tied to the risk-sharing avenues available to firm owners. In contrast to the commonly studied bond-only economy with collateral constraints (for example Moll (2014)), we find that the degree of misallocation is increasing in persistence of the idiosyncratic risk when firms have access to state-contingent contracts. The possibility to transfer wealth from high productivity states to low productivity states allows firm owners to trade off efficient allocation of consumption against efficient allocation of capital. We show that for reasonable values of risk aversion, insurance needs more than offset production efficiency concerns and thereby generates large capital misallocation.
Cellulitis and erysipelas

This chapter explores the clinical presentation, microbiology, and treatment of cellulitis and erysipelas. Erysipelas is a superficial skin infection limited to the upper dermis and superficial lymphatics with a distinct clinical presentation. The legs are the most commonly affected site, but erysipelas can occur anywhere on the body. Meanwhile, cellulitis is a type of acute bacterial skin and skin-structure infection (ABSSSI) that involves the deep dermis and extends into the subcutaneous tissue. Clinically, cellulitis presents as a unilateral, ill-defined, erythematous, firm plaque in conjunction with the four cardinal signs of inflammation: rubor, calor, dolor, and tumor. Skin manifestations are often preceded by fever, chills, and malaise. Though there are notable differences between erysipelas and cellulitis, the treatment is similar and typically based on disease severity and presence of purulence. Systemic antimicrobial therapy is the treatment of choice for cellulitis/erysipelas. In addition to antimicrobial therapy, local measures including immobilization and elevation as well as supportive measures such as warm compresses can hasten recovery and alleviate symptoms.
Gelenkbeteiligung bei Akromegalie

Zusammenfassung Die Akromegalie ist eine seltene Erkrankung, welche in den meisten Fällen aus einer hypophysären Überproduktion von Wachstumshormon (GH – Growth Hormone) resultiert. Der in der Folge anhaltende IGF-1(Insulin like Growth Factor-1)-Exzess bewirkt zahlreiche morphologische und metabolische Veränderungen des Organismus inklusive Organomegalie, arterieller Hypertonie und Diabetes mellitus. Insbesondere die kardiovaskuläre Morbidität nimmt zu, diese ist auch hauptverantwortlich für vermehrte Sterblichkeit der Betroffenen. Wiewohl nicht prognoseentscheidend, manifestiert sich die Erkrankung gleichfalls am Bewegungsapparat. Drei Komplikationen sind relevant: eine Arthropathie größerer Gelenke, lumbale Wirbelsäulenveränderungen sowie das Karpaltunnelsyndrom. Die Behandlung dieser Komplikationen unterscheidet sich nicht von der Therapie anderer Krankheitsmanifestationen: entscheidend ist die Beseitigung des GH-/IGF-1-Exzesses. Je früher die Diagnose gestellt und die Behandlung eingeleitet wird, desto besser ist die Prognose, sind doch ossäre/artikuläre Veränderungen in vielen Fällen irreversibel.
PERSPECTIVE Acanthamoeba Keratitis: Diagnosis and Treatment Update 2009

Acanthamoeba keratitis (AK). ● DESIGN: A perspective based on the literature and author experience. ● RESULTS: Early diagnosis and appropriate therapy are key to a good prognosis. A provisional diagnosis of AK can be made using the clinical features and confocal microscopy, although a definitive diagnosis requires culture, histology, or identification of Acanthamoeba deoxyribonucleic acid by polymerase chain reaction. Routine use of tissue diagnosis is recommended, particularly for patients unresponsive to treatment for AK. Topical biguanides are the only effective therapy for the resistant encysted form of the organism in vitro, if not always in vivo. None of the other drugs that have been used meet the requirements of consistent cysticidal activity and may have no therapeutic role. The use of topical steroids is controversial, but probably beneficial, for the management of severe corneal inflammatory complications that have not responded to topical biguanides alone. The scleritis associated with AK is rarely associated with extracorneal invasion and usually responds to systemic anti-inflammatory treatment combined with topical biguanides. Therapeutic keratoplasty retains a role for therapy of some severe complications of AK but not for initial treatment. With modern management, 90% of patients can expect to retain visual acuity of 6/12 or better and fewer than 2% become blind, although treatment may take 6 months or more. ● CONCLUSIONS: Better understanding of the pathogenesis of the extracorneal complications, the availability of
A case-control study on various risk factors causing coronary artery disease among patients of the selected hospital in Hail region, Kingdom of Saudi Arabia

Certain risk factors increase the probability of developing coronary artery disease and it increases the number of death all over the World. The aim of this study is to identify the various risk factors in cases among subjects with coronary artery disease and in the control population who are unaffected with coronary artery disease and aimed to estimate the odds ratio among cases and controls. A case-control Epidemiological observational study was used. Sample Case: Patients with coronary artery disease were included. Control: Matched outpatients who don’t have coronary artery disease and fulfill the inclusion criteria. The sample size of this study in each group was 40. For Cases Simple random sampling technique was used and Controls are chosen randomly who were matching with the case group. Smoking acts as the strongest risk factor with p-value zero, so the odds of having CAD is 31 times in smokers than non-smokers. This is followed by anti-hypertensive Drug which has an odd of CAD with 10.5 times on patients taking anti-hypertensive drugs than those who do not take such drugs. The studied population has a high prevalence and clustering of traditional risk factors.
Complete Remission of Metastatic Melanoma with Nivolumab and Complete Remission of Hepatitis C with Ledipasvir

Trials for programmed death 1 receptor (PD-1) inhibitors to treat melanoma have excluded patients with chronic hepatitis C virus (HCV) infection. Here, we describe a patient with metastatic melanoma and co morbid chronic HCV, which had previously failed interferon therapy. Complete remission of metastatic melanoma was achieved after 1 year of therapy with PD-1 inhibitor nivolumab, after progression of melanoma on cytotoxic T-lymphocte antigen 4 (CTLA-4) inhibitor ipilimumab. The patient’s HCV viral load remained elevated but stable during treatment with iplimumab and nivolumab. Following complete remission of melanoma with nivolumab, the patient underwent 12 weeks of targeted HCV therapy with ledipasvir and sofosbuvir, resulting in a complete virological response. The patient has ongoing complete remission of metastatic melanoma at 2 years and 9 months of nivolumab and an undetectable HCV viral load. This case highlights the ability to safely achieve a good clinical outcome for co morbid metastatic melanoma and chronic HCV with PD-1 inhibitors and new targeted HCV treatment.
Infiltrasi Limfosit dan Makrofag di Lamina Propria dari Ileum Ayam Pedaging dengan Penambahan Aloe Vera pada Pakan

Aloe vera adalah salah satu fitofarmaka yang dapat digunakan sebagai imunomodulator. Khasiat lidah buaya sebagai obat herbal telah diketahui sejak 1500 sebelum masehi. Pengaruh pemberian lidah buaya pada pakan ayam pedaging terhadap infiltrasi makrofag dan lmfosit pada lamina propria ileum diamati melalui pemeriksaan preparat histologi ileum. Hewan coba yanng digunakan adalah 32 ekor ayam pedaging yang dibagi dalam 8 kelompok perlakuan yang terdiri dari 2 group kontrol, 3 kelompok perlakuan kering dengan dosis 0,5%, 1% dan 1,5%; serta 3 kelompok perlakuan basah dengan dosis 0,5%, 1% dan 1,5%. Data yang diperoleh diuji dengan Anova pola faktorial dilanjutkan dengan uji Duncan. Pada penelitian ini tidak didapatkan interaksi antara bentuk (kering dan basah) Aloa vera namun memberikan pegaruh terhadap jumlah limfosit lamina propia ileum pada perbedaan pemberian dosis. Penambahan Aloe vera tidak mengakibatkan peningkatan jumlah makrofag lamina propria namun diguga berperan dalam aktivasi kerja dari makrofag. Berdasarkan hasil penelitian, dapat disimpulkan bahwa Aloe vera dalam bentuk kering dan basah dengan menggunakan tiga tingkatan dosis pada ayam pedaging dapat meningkatkan infiltrasi limfosit di lamina propria dari ileum tanpa mempengaruhi jumlah infiltrasi makrofag.
THE IMPACT OF SOCIAL BUSINESS PLATFORM ON CONSUMER LOYALTY: A CASE STUDY OF ZHEJIANG FRESHHERMA AS AN EXAMPLE MARKET

2019 the community business platform in mainland China more and more popular. Mainly because of the functionalities of social business platform innovation, community business links are constantly changing, creating a new business community platform business model. Many scholars have neglected the consumer loyalty of the community in the study community business, the community is through the circle of friends of word of mouth, trust, information transmission, to build consumer loyalty to the community business platform. The number of samples in this study was N = 471, and the impact of the social commerce platform function on consumer loyalty was investigated through investigation. The object of our investigation is to freshherma as the object of investigation, the ultimate goal is to understand their consumer platform for the use of the situation. Keyword: community commerce, word of mouth, trust, consumer loyalty.
The Role of Adult Hippocampal Neurogenesis in Depression-Relevant Reward-Related Behaviours

The variability in the symptomatology of depressive disorders and antidepressant treatment response has led to an increased interest in the molecular, cellular, and circuit mechanisms of many aspects of affect. Evidence suggests a reduction in adult hippocampal neurogenesis (AHN) is associated with an increase in depression-like behaviour, though much of this evidence has been from studies using aversive tests (e.g., forced swim test). Here, I used touchscreen operant chambers, which allow for non-aversive and translational testing, to test the hypothesis that AHN plays a contributing role in emotion regulation. A panel of three touchscreen tests were chosen to assess different aspects of depression-relevant reward-related behaviour, namely probabilistic reversal learning, progressive ratio, and extinction learning. Results across these tests largely indicate that AHN knockdown does not affect sensitivity to feedback information, motivation across a variety of reward strengths, and the ability to cease responding after reward withdrawal. Therefore, it seems that a role of AHN in emotion regulation may only apply in stressful, but not non-aversive, conditions.