Miracle-dz/UniFAR-FTUs huggingface dataset:
Purification, Crystallization and Properties of NADP+ -Specific Glutamate Dehydrogenase from Lactobacillus fermentum

The distribution of amino acid dehydrogenases in lactic acid bacteria was investigated. Several lactic acid bacteria were found to have NADP+-specific glutamate dehydrogenase, but NAD+-specific glutamate dehydrogenase activity was not detected in the bacteria tested. No alanine, leucine or lysine dehydrogenase activity was detected either, though serine and proline dehydrogenases occur in some of the bacteria. NADP+-specific glutamate dehydrogenase was purified to homogeneity and crystallized from Lactobacillus fermentum IFO 3071. The enzyme had a molecular weight of about 300,000 and consisted of six subunits identical in molecular weight (50,000). The enzyme is highly specific for l-glutamate and a-ketoglutarate, and the activity of the reductive amination is much higher than that of the oxidative deamination. The pH optima for the deamination and the amination were about 9.0 and 8.0, respectively. The apparent Km values were determined to be 79 mm for l-glutamate, 44µm for NADP+, 5.6 mm for α-ketogluta.
ROLE ON EMPLOYEES’ ATTITUDE IN WORK PLACE

In recent days importance of employees and their attitude is getting attention; this made numerous studies to analyze the behavior of individual in workplace. Andrade and Drake (2009) also affirm that managing human resource is essential in current scenario. With the methodology of Chronological study of reviews, this study was conducted to assess the behavioral of individuals in workplace, especially focused on job satisfaction, job involvement and organizational commitment. The researcher fined significant relationship between job satisfaction, job involvement and organizational commitment.
Productivity of enterotoxins and toxic shock syndrome toxin-1, and coagulase type of Staphylococcus aureus strains isolated from bovines and humans in the same district.

限局した一地方で得られたウシ乳房炎から分離した黄色ブドウ球菌の毒素産生性パターンと,同じ地域の病院患者から得られた黄色ブドウ球菌が同じパターンをもっているかどうかを検討し,地域的に同じパターンの黄色ブドウ球菌がウシとヒトとの感染症に分布している可能性をみた. 用いた黄色ブドウ球菌は,1990年10月から1993年9月までの間に千葉県館山市付近における132の酪農家のウシ乳房炎乳から分離した290株と,1992年11月から1993年3月の間に,病院患者から分離した131株である. その結果,1) ウシ乳房炎乳からの分離株では,コアグラーゼVI型が最も多く,毒素は57. 9が産生していてエンテロトキシン(SEA～D)が51. 7%,毒素性ショック症候群毒素-1(TSST-1)が31. 7%を占めていた. エンテロトキシン産生株中でSECが47. 3%と最も多かった. 2) ヒト臨床分離株では,コアグラーゼはII型が最も多く,ウシ株に多かったVI型はなかった. 毒素は73. 3%が産生していてエンテロトキシンが68. 7%,TSST-1が45. 0%を占めていた. エンテロトキシン産生株中でSECが54. 4%と最も多かった. 3) コアグラーゼ型と毒素産生の組合せで最も多かった菌株は,ウシではVI型,SEC,TSST-1の組合せで全体の19. 3%,ヒトではII型,SEC,TSST-1の組合せが全体の36. 6%であった. 4) 卵黄反応は,ウシ株およびヒト株でそれぞれ68. 5%および96. 2%の陽性割合であった. 前者ではVI型,SEC,TSST-1株の97. 8%が陰性であったのに対して,後者ではII型,SEC,TSST-1株の95. 8%が陽性であった. 5) 同じ地域におけるヒト臨床分離株とウシ乳房炎からの分離株との間に,毒素産生プロフィールの共通性は認められなかった.
Evaluation of TiO2 and SnO Supported on Graphene Oxide (TiO2-GO and SnO-GO) Photocatalysts for Treatment of Hospital Wastewater

The effectiveness of two photocatalysts, TiO2 and SnO, supported on graphene oxide (TiO2-GO and SnO-GO) on the removal of organic matter from hospital wastewater effluent was evaluated at laboratory scale. The results of the experimental design allow us to conclude that variables such as catalyst type and catalyst concentration have a significant effect on the organic matter removal efficiency of the photocatalytic process. The highest levels of removal efficiencies—for chemical oxygen demand, 85%, for phenols, 80%, and for dissolved organic carbon, 94%—were achieved using a TiO2-GO catalyst with a concentration in the wastewater of 1.5 g/L.
Negative Reviews, Positive Impact: Consumer Empathetic Responding to Unfair Word of Mouth

This research documents how negative reviews, when perceived as unfair, can activate feelings of empathy toward firms that have been wronged. Six studies and four supplemental experiments provide converging evidence that this experienced empathy for the firm motivates supportive consumer responses such as paying higher purchase prices and reporting increased patronage intentions. Importantly, this research highlights factors that can increase or decrease empathy toward a firm. For instance, adopting the reviewer’s perspective when evaluating an unfair negative review can reduce positive consumer responses to a firm, whereas conditions that enhance the ability to experience empathy—such as when reviews are highly unfair, when the identity of the employee is made salient, or when the firm responds in an empathetic manner—can result in positive consumer responses toward the firm. Overall, this work extends the understanding of consumers’ responses to word of mouth in the marketplace by highlighting the role of perceived (un)fairness. The authors discuss the theoretical and practical implications of the findings for better management of consumer reviews.
Design of Wideband Differentially Fed Multilayer Stacked Patch Antennas Based on Bat Algorithm

A new approach for the design of wideband multilayer stacked patch antennas is proposed based on the bat algorithm. Differential topology is described for multilayer stacked patch antennas to achieve the stable and symmetry radiation patterns as well as the high cross-polarization (XP) suppression within the design band. The fabricated antenna prototypes show 25.5% and 34.9% impedance bandwidth for the two-layer and three-layer designs, respectively. The measured results validated the design.
Injection of total genomic DNA and restoration of wild type phenotype in trichocyst non-discharge mutant TND of Paramecium caudatum

To restore wild type phenotypes in mutants controlled by recessive genes, microinjection of whole genomic DNA into recipient macronucleus in Paramecium caudatum was performed. Donor DNA carrying three wild-type genetic markers (exocytosis, behavior and mating type) was introduced into a recipient cell which was a triple recessive homozygote for the corresponding genes. Of the three phenotypes, a trichocyst non-discharge mutant TND converted to wild type by microinjection of not only total genomic DNA but also fractionated restriction fragments, although the restored phenotype was not complete. This transformation was more efficient when injection was performed by using the recipient cells of S phase, compared with the cells of G1 phase. This successful restoration of wild type phenotype gives us hope for cloning mutated genes, especially when no information about gene products is available. We discuss the advantage of this total genomic DNA injection and the possibility of target gene cloning using this strategy.
Review : Mapping Brain Pathophysiology and Higher Cortical Function with Magnetic Resonance Imaging

Advances in magnetic resonance imaging (MRI) have moved the technology beyond its application solely as a diagnostic test to become a tool for addressing questions of in vivo pathophysiology and higher cortical function in humans. Diffusion-weighted MRI measures the apparent rate of translational movement of water molecules through brain parenchyma. This measurement can be used to determine axonal orientation within white matter, to define regions of tissue edema, and to permit early identification of ischemic neuronal injury related to impairment of Na+-K +-ATPase activity in experimental and human stroke. Changes in various aspects of cerebral perfusion—blood volume, blood flow, and hemoglobin oxygen saturation—can be mea sured with MRI, and altered cerebrovascular circulation and regional brain activation can thereby be inves tigated. Echo planar imaging is a method of ultrafast data acquisition with MRI—individual images are ac quired on the order of 100 msec. Echo planar imaging makes diffusion and perfusion measurements more practicable for diverse applications and allows for the study of temporal characteristics of regional brain responses to stimuli. Diffusion and perfusion MRI, generally termed functional MRI, are tools for studying in vivo brain physiology with MRI and are being applied to a broad range of questions in neuroscience. The Neuroscientist 1:221-235, 1995
Long Non-Coding RNA 691 Regulated PTEN/PI3K/AKT Signaling Pathway in Osteosarcoma Through ﻿miRNA-9-5p

Background Large amounts of researches indicate that non-coding RNAs play a crucial role in many malignancies. However, the potential mechanisms of non-coding RNAs involved in osteosarcoma tumorigenesis remain elusive. Materials and Methods The expression of long non-protein coding RNA 691 (lncRNA 691) in cell lines and paired osteosarcoma tissues was compared by qRT-PCR assay. Then, we explored the tumor suppressor function of lncRNA 691 with MTS and colony formation assay. Flow cytometry results showed lncRNA 691 can enhance cell apoptosis. Then, we predicted and verified the negative regulation relationship with miRNA and the miRNA’s target gene. Lastly, we revealed the tumorigenesis function of lncRNA-691/miRNA/target gene axis in osteosarcoma. Results In our study, we disclosed that lncRNA 691 had low expression levels in osteosarcoma cell lines and tissues. Overexpression of lncRNA 691 could suppress the cell proliferation and induce cell apoptosis in MG-63 cell line. Then, bioinformatics analyses were performed and miR-9-5p was found to negatively regulate the lncRNA 691 expression and promote the osteosarcoma tumorigenesis in vitro. PTEN was predicted as the target gene of miR-9-5p. Luciferase reporter assay and RIP assay demonstrated the regulatory network of lncRNA 691/miR-9-5p/PTEN. We revealed that PTEN was downregulated by the overexpression of miR-9-5p and upregulated by the overexpression of lncRNA 691. At last, the apoptosis﻿-associated protein of the lncRNA 691/miR-9-5p/PTEN/PI3K/AKT was further demonstrated. Conclusion LncRNA 691/miR-9-5p could regulate the tumorigenesis by regulating the PTEN/PI3K/AKT signal pathway in osteosarcoma.
The reniculus of the sei whale (Balaenoptera borealis)

Summary For the first time the histological structure of the reniculus of the sei whale (Balaenoptera borealis) is described, with special reference to its vasculature and to the constitution of its well-developed sporta. Renicular venous return is principally effected through a peripheral plexus whence relatively large interrenicular veins drain into the vv. reniculares, or, possibly, into the v. renalis as direct radicles. The material examined affords no evidence as to the occurrence of veins ‘wandering’ from the kidney surface directly to renal vein or postcava: the presence of such veins is not, however, thereby excluded. In its general structure the sei whale reniculus is essentially similar to the reniculus of other balaenopterids examined, though its sporta is relatively better developed.
Fine Structure and Histochemistry of the Choroid Plexus of the Teleost Leuciscus rutilus

Analyses of the fine structure of the posterior choroid plexus in the teleost Leuciscus rutilus and the determination of the presence and function of the enzymes acid and alkaline phosphatases, ATPase and glucose-6-phosphatase confirm similarities between these epithelial cells and the saccus dorsalis and also with the epithelial cells of the choroid plexus found in mammals. The teleost plexus cells contain coated vesicles which are derived from the plasmalemma as well as from the Golgi complex. Moreover, they contain multivesicular bodies and Iysosomes. These organelles function in the absorption of substances from the cerebrospinal fluid and in the breakdown of these substances within the cells. The investigated enzymes play an important role in the secretion of electrolytes into the cerebrospinal fluid by active membrane transport.
Occurrence of Vesicular Mycorrhizal Fungi in Dryland Species of Restionaceae and Cyperaceae From South-West Western Australia

Species of Cyperaceae and Restionaceae were examined for presence of vesicular-arbuscular (VA) mycorrhizal fungi in natural habitat in south-west Western Australia. VA mycorrhizal fungi were detected in roots of two species of Cyperaceae (Lepidosperma gracile and Tetraria capillaris), and two species of Restionaceae (Alexgeorgea nitens and Lyginia barbata), all representing the first records for these genera. Results indicated a very short seasonal period of infection, with VA mycorrhizal fungi representing the genera Acaulospora, Glomus, Scutellospora and Gigaspora identified in roots. VA mycorrhizal fungi were prominent from late autumn to early winter (April-June) and in up to 30% of the young, new season's roots as they penetrated the upper 10 cm region of the soil profile. Mycorrhizal infection was not evident during the dry summer months. This study suggests that mycorrhizas may be important for nutrition of these hosts in these environments but their activity is restricted to a brief period of the growing season.
Diallel Analysis of the Inheritance of Partial Resistance to Downy Mildew in Peas

The aim of the investigation was to see if the partial resistance of three sister-lines is determined by identical genes. The sister-lines were selected from a cross between a susceptible line and a partially resistant line. These lines and the susceptible parent line were crossed in all directions. The downy-mildew resistance of the different combinations was investigated in the F1, F3 and F4 generations. The resistance of the sister-lines is not determined by the same genes. The moderate resistance of line X309 is recessive and might be determined by a single gene. The more resistant line X282 carry at least one resistance gene that shows dominance in crosses with X309. Line X311 carries, in addition to the resistance in X309, some resistance gene or genes with an intermediate effect.
DPIR-Net: Direct PET Image Reconstruction Based on the Wasserstein Generative Adversarial Network

Positron emission tomography (PET) is an advanced medical imaging technique widely used in various clinical applications, such as tumor detection and neurologic disorders. Reducing the radiotracer dose is desirable in PET imaging because it decreases the patient’s radiation exposure. However, reducing the dose can also increase noise, affecting the image quality. Therefore, an advanced image reconstruction algorithm based on low-dose PET data is needed to improve the quality of the reconstructed image. In this article, we propose the use of a direct PET image reconstruction network (DPIR-Net) using an improved Wasserstein generative adversarial network (WGAN) framework to enhance image quality. This article provides two main findings. First, our network uses sinogram data as input and outputs high-quality PET images direct, resulting in shorter reconstruction times compared with traditional model-based reconstruction networks. Second, we combine perceptual loss, mean square error, and the Wasserstein distance as the loss function, which effectively solves the problem of excessive smoothness and loss of detailed information in traditional network image reconstruction. We performed a comparative study using maximum-likelihood expectation maximization (MLEM) with a post-Gaussian filter, a total variation (TV)-norm regularization, a nonlocal means (NLMs) denoising method, a neural network denoising method, a traditional deep learning PET reconstruction network, and our proposed DPIR-Net method and evaluated the proposed method using both human and mouse data. The mouse data were obtained from a small animal PET prototype system developed by our laboratory. The quantitative and qualitative results show that our proposed method outperformed the conventional methods.
Climate and the fisheries off Tasmania — interactions of physics, food chains and fish

The impact of climate on the fishery for jack mackerel Trachurus declivis off Tasmania has been traced to an interaction of the 40-day oscillation in the Zonal Westerly Wind (ZWW), driven by latitudinal changes in the position of the subtropical high pressure cells over south-eastern Australia, with ocean circulation and water column stability. Nitrate concentrations are strongly correlated with temperature in these waters. High ZWW stress causes advection of colder, nutrient-rich subantarctic water up the eastern side of Tasmania and reduces water column stability. The result is periodic overturn of the water column and increased new production. Spring blooms tend to be later, but stronger, as a result of periodic mixing and restratification. Reduced ZWW stress (under the influence of high pressure) leads to incursions of subtropical water, increased water column stability and reduced biological production. In calm years spring blooms tend to be earlier, but weaker, because of the reduced nitrate availab.
Some Chronic Effects of 2,4-D on the Bluegill (Lepomis macrochirus)

Abstract Bluegills (Lepomis macrochirus) were exposed to the propylene glycol butyl ether ester of 2,4-D in six ponds at Tishomingo, Oklahoma, one concentration per pond in five ponds, and a control pond. The fish and the pond environment were studied for five months to measure chronic effects on the fish and persistence of the herbicide in pond water, bottom sediments, and aquatic vegetation. Small quantities of 2,4-D were found in pond waters for six weeks after application. Residues of 2,4-D were found in vegetation and in bottom sediments in the highest-treated pond for six weeks. No 2,4-D was detected in fish after four days. One-fifth of the fish treated at 10 ppm died within 8 days; mortality was almost negligible among bluegills exposed to 5 ppm or less. Spawning among bluegills treated at 10 and 5 ppm was delayed about two weeks. Growth was faster among bluegills in these high-treatment ponds than in the low-treatment ponds which received 1.0, 0.5, and 0.1 ppm 2,4-D. Pathologic lesions were seen .
AGE-DEPENDENT CHANGES IN PITUITARYGONADAL RELATIONSHIPS

Pituitary levels of fsh (Steelman-Pohley assay) and lh (Parlow assay) were determined in male rats of various ages. The lh level began to rise by Day 33 of life, reached a peak between Days 43 and 47, and declined in the post-puberal period. The fsh level (\g=m\g/mgand \g=m\g/pituitary/100g body weight) began to rise very much earlier and had already reached its peak around Day 33, when testicular growth was rapid but before full spermatogenesis was established. In post-puberal rats, the fsh levels were much lower than those reached before puberty. The implication of these findings and the possibility that fsh and lh
CYTOLOGICAL STUDIES IN THE GENUS SILENE L.

Fourteen species of Silene in sections Siphonomorpha and Auriculatae were investigated cytologically and karyotypes prepared. Chromosome counts of six species are given for the first time. SAT-chromosomes were found in S. viridiflora and S. nodulosa. S. boryi and S. vallesia were found to be tetraploids.
Control of premature fruit drop in Macadamia integrifolia: effects of naphthalene acetic acid application, cincturing, and shoot-tip removal

The effects of NAA application, cincturing and shoot-tip removal on the incidence of premature fruit drop were investigated in orchard-grown trees of Macadamia integrifolia cv. Kakea. A single, post-anthesis application of NAA (1 ppm) increased (P < 0.05) the final set of macadamia fruit by 35%. Limb cincturing combined with shoot-tip removal increased (P < 0.05) initial fruit set and possibly final set. Cincturing alone was less effective and shoot-tip removal had no effect alone.
FEA Study of Shear Mode Decoupling in Nonstandard Thin Plates of a Lead-Free Piezoelectric Ceramic

The finite-element analysis (FEA) is used in this work to study the impedance curves and modes of motion at resonance of nonstandard shear plates, thickness poled, and longitudinally excited. An ecological, lead-free, piezoelectric ceramic of (<inline-formula> <tex-math notation="LaTeX">$1-{x}$ </tex-math></inline-formula>)(Bi<sub>0.5</sub>Na<sub>0.5</sub>)TiO<sub>3</sub>–<inline-formula> <tex-math notation="LaTeX">${x}$ </tex-math></inline-formula>BaTiO<sub>3</sub> with <inline-formula> <tex-math notation="LaTeX">${x}\,\,=0.06$ </tex-math></inline-formula> (BNBT6) composition is studied. The FEA modeling is based on the full matrix of the material coefficients. These are obtained from complex impedance measurements on two-thickness poled resonators. A study as a function of the variations of the dimensions of the plate was accomplished (<inline-formula> <tex-math notation="LaTeX">${t}\,\,=$ </tex-math></inline-formula> thickness for poling and <inline-formula> <tex-math notation="LaTeX">${L}$ </tex-math></inline-formula> and <inline-formula> <tex-math notation="LaTeX">${w}\,\,=$ </tex-math></inline-formula> lateral dimensions, where <inline-formula> <tex-math notation="LaTeX">${w}$ </tex-math></inline-formula> is the distance between electrodes for the electrical excitation). We aimed to a further understanding, and, thus, the ability to control, the coupling of the main shear resonance and the lateral modes. The use of uncoupled shear modes to obtain the material parameters is a key issue for their determination as complex quantities, thus considering all material losses, electromechanical, dielectric, and elastic.
Survival and splash dispersal of Phytophthora parasitica, causing dieback of rhododendron

Effets de l'humidite sur la survie et sur la sporulation de P.p. dans les tissus foliaires des rhododendrons infectes. Caracterisation du role de l'eau des gouttelettes d'eclaboussure dans la dissemination des propagules du champignon en conditions de pepiniere
Maximizing the utility of peer support in carceral settings: A few stumbling blocks to consider

Emerging research advocates prison-based peer support programmes not only for the recipients of support but also for those providing it. Such programmes are founded on principles such as reciprocity, shared problem-solving and empathy. Accordingly, there have been recent claims that such structures may engender a magnified impact in carceral settings characterized by deprivation and adversity. Specifically, it has been argued that peer supporters garner opportunities to enact prosocial behaviours and consequently energize desistance narratives while serving time. However, as intrigue and optimism around this untapped resource grow, so too does the need to explore any hindrances that might halt progressive developments. This article presents data from qualitative interviews held with incarcerated peer supporters in the UK. Transcripts of the institutional challenges that participants faced when undertaking their roles were thematically analysed, and suggestions for practitioners are offered. The article calls for professionals and policy makers to further explore the redemptive potential of prison-based peer support.
Hydrophobicity of the cells of fast- and slow-growing bacteria isolated from a grassland soil

The cell surface tension of 250 strains of bacteria isolated from grassland soil was examined by measuring the contact angle of a liquid droplet on the cell layer. The values of the contact angle of aqueous 0.1M NaCl solution and α-bromonaphthalene on the cell layer were used to calculate the polar and non-polar components of surface tension for the isolates. The polar component of the "slow-growing" isolates was smaller than that of the "fast-growing" isolates. On the other hand, no difference could be observed in the non-polar component between the fast- and slow-growing isolates. These results indicate that slow-growing isolates have more hydrophobic cell surface than fast-growing isolates.
Deferring, Deliberating, or Dodging Review

While panel effects—instances in which panel composition affects the votes cast by judges—have been widely documented, scholars are unsure why these patterns persist. We outline three possible mechanisms, acquiescence, deliberation, and strategy, through which panel effects might occur; develop indicators for each; and test them using a data set of search and seizure cases decided by the US courts of appeals between 1953 and 2010. Our analysis provides some evidence that counterjudge success stems from a combination of all three theories, although strategic considerations have the substantively strongest and most consistent effects.