Miracle-dz/UniFAR-FTUs huggingface dataset:
Attenuated Vascular Reactivity in Dogs With Anteroventral Third Ventricle Lesions

Lesion of the anteroventral portion of the third cerebral ventricle causes hypernatremia, adipsia, and attenuation of the pressor response to intravenous administration of angiotensin II and norepinephrine. In addition, these lesions prevent the development of several experimental models of hypertension. In this study, a lesion of the third cerebral ventricle region was made in 14 dogs. In seven dogs in which hypernatremia developed the lesions included the organum vasculosum of the lamina tenninalis; seven animals in which the circumventricnlar organ was spared by the lesion remained normonatremic Vascular responsiveness of isolated right carotid artery rings to angiotensin II and phenylephrine was assessed 3 days after lesioning the anteroventral portion of the third cerebral ventricle. In endothelium-denuded ring vessels, vasoconstrictor responses to phenylephrine were significantly decreased in animals both with and without inclusion of the organum vasculosum of the lamina tenninalis. A similar effect was observed in intact vessels of dogs in which the circumventricular organ was spared but not in those with lesions that included this area. In contrast, angiotensin Il-induced vasoconstriction was significantly decreased in the arteries with intact endothelium of both groups of lesioned animals. These data show that lesion of the anteroventral third ventricle area alters α-adrenergic and angiotensin II vascular responsiveness in isolated carotid artery rings with the possible participation of the endothelium.
Bone‐Marrow and Fetal‐Liver Transplantation in Immunodeficiencies and Inborn Errors of Metabolism: Lack of Significant Restriction of T‐Cell Function in Long‐Term Chimeras Despite HLA‐Mismatch

Bone-marrow-transplantation (BMT) was initially developed as a treatment for severe combined immunodeficiency disease (SCID), aplastic anemia and acute leukemia. Because of progressively improved results, this form of therapy has been used more recently in a larger variety of immunodeficiencies, hemopathies and inborn errors of metabolism. Several reports in this volume deal with BMT in leukemias, aplastic anemias and other hemopathies. This paper concentrates on clinical and immunological features of BMT in immunodeficiencies. To avoid repetition, special focus has been directed toward data provided by our patients. This review also describes, in detail, the results of an alternative form of therapy of great interest, which we have investigated and developed: fetal tissue transplantation, a treatment understandably applied first to immunodeficiencies, but with a potential for much wider application. In virtually all cases, transplantation of fetal tissue is made from an "HLA-different" donor and this circumstance has enabled us to investigate the possible role of HLA-mismatch in restricting certain T-<;ell functions in vivo, in man. A hypothesis is proposed to reconcile apparent discrepancies between data from murine experimental
Design and Testing of a Controller for Autonomous Vehicle Path Tracking Using GPS/INS Sensors

Abstract This paper describes a steering controller integrated with speed controller for autonomous path tracking using GPS and INS sensors. The steering controller for path tracking is developed based on the finite preview optimal control method. The steering control input is computed using the road information within preview distance. The speed controller determines the speed command necessary to maintain a lateral acceleration limit and improve vehicle safety. The vehicle model for simulation study is validated using vehicle test data. Finally, the controller is implemented on a by-wire vehicle, P1, to validate the performance of the steering controller integrated with speed controller.
Enhanced and coordinated in vivo expression of inflammatory cytokines and nitric oxide synthase by chondrocytes from patients with osteoarthritis.

OBJECTIVE To evaluate the sites of expression of interleukin-1beta (IL-1beta), tumor necrosis factor alpha (TNFalpha), and inducible nitric oxide synthase (iNOS) in patients with inflammatory and degenerative joint diseases. METHODS Cytokines and iNOS were detected by immunohistochemistry analysis of synovial and cartilage biopsy specimens obtained at knee arthroscopy in patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), osteoarthritis (OA), and traumatic knee arthritis. Cytokine and iNOS expression was quantified using computerized image analysis. RESULTS IL-1beta, TNFalpha, and iNOS were highly expressed by synovial cells (lining layer cells, infiltrating leukocytes, endothelial cells) from patients with inflammatory arthritides and significantly less by synovial cells from patients with OA and traumatic arthritis. In contrast, the latter patients showed high chondrocyte expression of cytokines and iNOS while RA and PsA patients had only minor chondrocyte positivity. In both joint compartments, IL-1beta expression, TNFalpha expression, and iNOS expression were strongly correlated. CONCLUSION The enhanced and coordinated expression of IL-1beta, TNFalpha, and iNOS by chondrocytes strongly supports the hypothesis that chondrocytes are the major site of production of mediators of inflammation in human OA, thus playing a primary role in the pathogenesis of this disease.
Lumbar commissural interneurons with reticulospinal inputs in the cat: Morphology and discharge patterns during fictive locomotion

The purpose of this study was 1) to characterize the morphology of lumbar commissural neurons (CNs) with reticulospinal inputs and 2) to quantitate their activity during locomotor rhythm generation. Intraaxonal recordings at the L4–7 level of the spinal cord were obtained in 67 neurons in the decerebrate, paralyzed cat. Fourteen of them were subsequently nearly fully visualized following their intraaxonal injection with the tracer neurobiotin. All 14 were CNs with axons projecting across the midline of the spinal cord. Their somata were located mainly in lamina VIII and additionally in laminae VII–VI. Most of the lamina VIII CNs were excited monosynaptically from reticulospinal pathways. They were judged to be interneuronal CNs if they had no, or a short, rostral projection. These CNs commonly gave off multiple axon collaterals in and around their somata's segmental level. They projected mainly to laminae VIII–VII and some additionally to lamina IX. Some laminae VIII and the laminae VII–VI CNs were excited polysynaptically from reticulospinal pathways or were not excited. They were judged to be long propriospinal or ascending tract CNs because they had only an ascending axon. Most lamina VIII CNs discharged rhythmically during fictive locomotion evoked by stimulation of the mesencephalic locomotor region, exhibiting one peak per locomotor cycle. The peak was in phase with neurographic activity of either a left or a right hindlimb extensor nerve. These results suggested that lamina VIII CNs are reciprocally connected bilaterally at each segmental level. Such an arrangement suggests their participation in the generation and coordination of reciprocal and bilateral locomotor activity. J. Comp. Neurol. 474:546–561, 2004. © 2004 Wiley‐Liss, Inc.
Infectious center assay for complementation and recombination between mutant of reovirus

An infectious center assay has been developed to measure recombination and complementation in L cells mixedly infected with ts mutants of reovirus. The mutants studied so far fall into complementation groups that correspond to the recombination groups previously defined by other laboratories (B. N. Fields, 1971; B. N. Filds and W. K. Joklik, 1969).
Goal disturbance predicts health-related quality of life and depression 4 months after myocardial infarction.

OBJECTIVES The aim of this longitudinal study was to determine whether the event of a myocardial infarction (MI) would lead to a disturbance in important higher-order goals, and whether goal disturbance could predict health-related quality of life (HRQL) and depression 4 months later, in addition to baseline scores, demographic characteristics, presence of anginal complaints (AP) or chest pain, coping strategies and social support. METHOD A total of 113 MI patients completed questionnaires shortly after hospitalization (T1) and 4 months later (T2), assessing (an impact of the event on) important higher-order goals (T1), disease-related coping strategies (T1), perceived adequacy of social support (T2), AP (T2), HRQL, and depression (T2). Two separate hierarchical regression analyses were performed with HRQL and depression at T2 as dependent variables. RESULTS The results suggest that the experience of an MI has an impact on the attainment of higher-order goals. Goal disturbance is an independent predictor of both HRQL and depression after MI. CONCLUSION A goal theory perspective can provide additional insights into HRQL outcomes after an MI.
Telomerase activity concentrates in the mitotically active segments of human hair follicles.

Telomerase is a ribonucleoprotein enzyme capable of adding hexanucleotide repeats onto the ends of linear chromosomal DNA. Whereas normal somatic cells with a limited replicative capacity fail to express telomerase activity, most immortal eukaryotic cells do. Cells of renewal tissues (e.g., skin, intestine, blood) require an extensive proliferative capacity. Some cells in such renewal tissues also express telomerase activity, most likely to prevent rapid erosion of their telomeres during cell proliferation. In this study, we measured the levels of telomerase activity in dissected compartments of the human hair follicle: hair shaft, gland-containing fragment, upper intermediate fragment (where it is thought undifferentiated stem cells reside), lower intermediate fragment, and in the bulb-containing fragment (an area with high mitotic activity containing a more differentiated pool of keratinocytes). In anagen follicles, high levels of telomerase activity were found almost exclusively in the bulb-containing fragment of the follicles, with low levels of telomerase in the bulge area (intermediate fragments) and gland-containing fragment. In comparison, catagen follicles had low levels of telomerase activity in the bulb-containing fragments as well as in other compartments. Such observations indicate that, in anagen hair follicles, the fragments containing cells actively dividing (e.g., transient amplifying cells) express telomerase activity, whereas fragments containing cells with low mitotic activity, for example, quiescent stem cells, express low levels of telomerase activity.
Hymenobacter arcticus sp. nov., isolated from glacial till.

A novel, red-pink-pigmented strain, designated R2-4(T), was isolated from a till sample near Ny-Alesund, Svalbard Archipelago, Norway. Cells were aerobic, Gram-stain-negative and rod-shaped. Growth occurred at 4-30 °C (optimum, 20-22 °C), at pH 6.0-9.0 (optimum, pH 7.0) and with 0-1% NaCl added to R2A agar. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R2-4(T) belonged to the genus Hymenobacter. 16S rRNA gene sequence similarity between strain R2-4(T) and the type strains of related species of the genus ranged from 94.51 to 96.05%. Strain R2-4(T) contained iso-C(15 : 0), anteiso-C(15 : 0), summed feature 3 (C(16 : 1)ω6c and/or C(16 : 1)ω7c), summed feature 4 (C(17 : 1) anteiso B and/or iso I) and C(16 : 1)ω5c as the major cellular fatty acids, MK-7 as the major respiratory quinone, and phosphatidylethanolamine, unknown aminophospholipids, unknown aminolipids and unknown lipids as the main polar lipids. The polyamine was sym-homospermidine. The DNA G+C content of strain R2-4(T) was 61.6 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain R2-4(T) is considered to represent a novel species of the genus Hymenobacter, for which the name Hymenobacter arcticus sp. nov. is proposed. The type strain is R2-4(T) ( = CCTCC AB 2012104(T) = KACC 16881(T)).
Effect Of Elbow And Wrist Joint Position On Grip Strength

Background and Objective: Grip and pinch strength measurements provide an objective index of the functional integrity of the upper extremity. Hand strength evaluation is importance in determining the effectiveness of different treatment strategies or effects of different procedures. As studies on the effect of elbow and wrist positions on grip strength remain controversial, the present study aimed to find out the effect of elbow and wrist joint positions on grip strength and the favorable and advantageous position to improve grasp function. Method: This study included 144 healthy normal subjects with 13 males and 131 females. A standard adjustable hydraulic hand dynamometer was used for measuring grip strength of various positions of elbow and wrist. Result: The result of the study showed that the elbow and wrist positions had impact on the grip strength when considered individually (p<.01). But the interaction effect of these two different positions could not able to show any significant difference. Conclusion: Grip strength was maximum with elbow in full extension and wrist in neutral. The changes in wrist position on grip strength were observed with variations in elbow and wrist positions.
Journal of Informetrics

Internationally co-authored papers are known to have more citation impact than nationally co-authored paper, on average. However, the question of whether there are systematic differences between pairs of collaborating countries in terms of the citation impact of their joint output, has remained unanswered. On the basis of all scientiﬁc papers published in 2000 and co-authored by two or more European countries, we show that citation impact increases with the geographical distance between the collaborating counties.
Apoptosis-inducing properties of Viscum album L. extracts from different host trees, correlate with their content of toxic mistletoe lectins.

BACKGROUND Extracts from Viscum album L. (VAL), which are one of the most widely used alternative cancer therapies in Europe, have been recognised to induce apoptosis. A clinically relevant direct anti-tumour effect might be induced only by their intratumoural or intrapleural injection, and thus effectiveness is highly dependent on the apoptosis-inducing potencies of the extracts used. MATERIALS AND METHODS To characterise these properties, human lymphocytes were incubated for 72 hours with VAL extracts from different host trees and harvest times. Cell death-associated changes such as an apoptotic sub-G1 peak, binding of Annexin-V, generation of reactive oxygen intermediates (ROI), expression of mitochondrial Apo2.7 molecules, and uptake of the DNA-intercalating dye propidium iodide were measured by flow cytometry. RESULTS AND CONCLUSIONS Biologically active VAL compounds, and thus cytotoxicity, are dependent on the manufacturing process, host tree, and time of harvest. Although the mistletoe lectin (ML) content of VAL extracts strongly correlated with their apoptosis-inducing properties, the presence of these proteins will not guarantee its biological activity, indicating the involvement of other components which may modulate ML cytotoxicity.
New Pharmacological Targets for Asthma Drug Development

Asthma is an inflammatory disorder characterized by airway hyperresponsiveness, followed by inflammation, remodeling and oxidative stress in the respiratory system and lung tissue. While glucocorticosteroids remain the gold-standard of asthma therapy, they have limitations because of their potentially severe adverse effects and the presence of corticosteroid resistance in some patients. In the present review we will focus in four main groups of experimental pharmacological approaches for future asthma and hyperresponsiveness treatment: proteinase inhibitors and flavonoids, arginase and iNOS inhibition, Rho-kinase inhibitors, cholinergic anti-inflammatory system and nicotinic receptors.
Advancements in Non Viral Gene Therapy for Hemophilia

Over the last 10-15 years, significant advances in vector design and delivery techniques have facilitated the development of nonviral approaches for the treatment of hemophilia. Despite these advancements, there remain several obstacles preventing the successful application of these approaches in larger mammals such as dogs and humans. This review covers nonviral gene therapy approaches using both in vivo gene delivery and ex vivo gene transfer. Plasmid-based approaches, as well as integrating transposons are examined for efficacy, risks and limitations. Results are presented on the only human clinical trial in hemophilia that utilized nonviral approaches.
Subunit characterization of NMDA receptors.

NMDA receptors are a subclass of excitatory, ionotropic L-glutamate neurotransmitter receptors. They are.heteromeric, integral membrane proteins being formed by the assembly of the obligatory NR1 subunit together with modulatory NR2 subunits of which four different types, NR2A-NR2D, have been described. This results in a heterogenous population of receptor proteins with distinct pharmacological and biophysical properties thus yielding potential for the development of NMDA receptor subtype-selective therapeutic agents. Anti-NMDA receptor subunit antibodies have been generated and used in immunoprecipitation or immunoaffinity purification studies to determine the in vivo subunit complements of NMDA receptors. This article summarizes knowledge on the subunit compositions of NMDA receptors based on these approaches together with the current status of NMDA receptor subunit stoichiometry and hence quaternary structure. of native NMDA receptors.
Towards Ensuring Eternal Connectability

The aim of the CONNECT Project is to achieve universal interoperability between heterogeneous Networked Systems. For this, the non-functional properties required at each side of the connection going to be established must be fulfilled. By the inclusive term "CONNECTability" we comprehend properties belonging to all four non-functional concerns of interest for CONNECT, namely dependability, performance, security and trust. In this paper we focus on approaches for analysis and monitoring of dependability and performance of CONNECTed systems, and their combined usage.
Laparoscopic Exploration Can Salvage Recurrent Common Bile Duct Stone after Cholecystectomy

Conventionally, patients suffered from recurrent common bile duct (CBD) stone after cholecystectomy are suggested to be treated with endoscopic retrograde cholangiopancreaticography. This study was designed to explore the feasibility of laparoscopic common bile duct exploration (LCBDE) as a salvage procedure for recurrent CBD calculi after cholecystectomy. A retrospective review was conducted of data from 65 patients who underwent LCBDE for recurrent CBD calculi after cholecystectomy from January 2011 to July 2015. LCBDE was successfully carried out in 61 cases, with a successful rate of 93.8 per cent. Three cases required open conversion because of serious abdominal adhesion, and one case for intraoperative bleeding. Postoperative bile leakage occurred in two cases, and bile peritonitis developed in one case; all these three patients with complications were fully cured by conservative treatment. A postoperative retained CBD stone was found in one patient, which was extracted with endoscopic sphincterotomy. Furthermore, it was found that the mean operative time and length of postoperative hospital stay were much shorter in primary closure group (n = 49) than in T-tube drainage group (n = 12), and the hospital expense was also lower in primary closure group. We suggest that LCBDE could be a novel approach as a salvage procedure for the recurrent CBD stone after cholecystectomy, and we prefer to intraoperative primary closure of CBD if possible.
LOS JUEGOS COMO HERRAMIENTA DOCENTE UNIVERSITARIA PARA EL CONOCIMIENTO CIENTÍFICO EN ESTUDIANTES DE BIOLOGÍA

Actualmente el uso de nuevas metodologias pedagogicas son necesarias en el contexto docente universitario, ya que ello supone un aliciente anadido a las tradicionales clases magistrales. Durante el curso 2013/14 se ha evaluado el uso de un juego virtual educativo, entendido como un instrumento para mejorar la difusion de diversos conceptos relacionados con la Ecologia del Paisaje en un entorno facil y agradable. La experiencia ha sido llevada a cabo en alumnos de la asignatura de Ecologia del Paisaje Ganadero de la Universidad de Sevilla, como herramienta de aprendizaje para apoyar esta ensenanza (bien como refuerzo, auto-evaluacion, etc.) y/o con posibilidad de incorporarlo a la plataforma virtual de la Universidad de Sevilla (Black Board). El juego consta de una serie de preguntas al azar que el jugador debe responder correctamente para avanzar, con diferentes niveles de dificultad (baja, media y alta). Puede jugar un solo jugador o varios a la vez y existe la posibilidad de que la interfaz este en espanol o en ingles. Los resultados obtenidos mostraron un alto indice de satisfaccion de los alumnos con el uso de este sistema de apoyo, asi como un incremento en la participacion e interaccion de los alumnos en la clase. La adaptacion futura a las diferentes asignaturas impartidas en el area o resto de areas de conocimiento tratadas en el actual Grado de Biologia solo implica cambiar el interfaz de usuario y los criterios de seleccion de las preguntas/videos.
Synthesis and Molecular Modelling of Novel Substituted‐4,5‐dihydro‐(1H)‐pyrazole Derivatives as Potent and Highly Selective Monoamine Oxidase‐A Inhibitors

This report describes novel pyrazoline derivatives investigated for their ability to selectively inhibit the activity of the A and B isoforms of monoamine oxidase. These new synthetic compounds proved to be reversible, potent, and selective inhibitors of monoamine oxidase‐A rather than of monoamine oxidase‐B, and are promising candidates to further advance drug discovery efforts. The most active compounds show inhibitory activity on monoamine oxidase‐A in the 1.0 × 10−8–8.6 × 10−9 mrange. Moreover, it should be pointed out that for some compounds a high IC50 ≥ 10−9 m value is associated with a high A‐selectivity (Selectivity Index monoamine oxidase‐B/monoamine oxidase‐A in the 10 000–12 500 range). Further insight to understand enzyme‐inhibitor molecular interaction was obtained by docking experiments with the monoamine oxidase‐A and monoamine oxidase‐B isoforms.
Biphasic growth dynamics during Caulobacter crescentus division

Cell size is specific to each species and impacts their ability to function. While various phenomenological models for cell size regulation have been proposed, recent work in bacteria have demonstrated an adder model, in which a cell increments its size by a constant amount between each division. However, the coupling between cell size, shape and constriction, remain poorly understood. Here, we investigate size control and the cell cycle dependence of bacterial growth, using multigenerational cell growth and shape data for single Caulobacter crescentus cells. Our analysis reveals a biphasic mode of growth: a relative timer phase before constriction where cell growth is correlated to its initial size, followed by a pure adder phase during constriction. Cell wall labeling measurements reinforce this biphasic model: a crossover from uniform lateral growth to localized septal growth is observed. We present a mathematical model that quantitatively explains this biphasic mixer model for cell size control.