Miracle-dz/UniFAR-FTUs huggingface dataset:
Learning Docker

Optimize the power of Docker to run your applications quickly and easily About This BookLearn to compose, use, and publish the Docker containersLeverage the features of Docker to deploy your existing applicationsExplore real world examples of securing and managing Docker containersWho This Book Is For If you are an application developer who wants to learn Docker in order to utilize its features for application deployment, then this book is for you. No prior knowledge of Docker is required.What You Will Learn Build a Docker image using Dockerfiles Push and publish images on Docker Hub Run your own private Docker Hub and upload images onto it Create and run services inside a container to deploy your applications with ease Share data between the Docker host and containers Orchestrate multiple containers with Docker Compose Test and debug applications inside a Docker container Secure your Docker containers with SELinux In DetailDocker is a next-generation platform for simplifying application containerization life-cycle. Docker allows you to create a robust and resilient environment in which you can generate portable, composable, scalable, and stable application containers.This book is a step-by-step guide that will walk you through the various features of Docker from Docker software installation to the impenetrable security of containers. The book starts off by elucidating the installation procedure for Docker and a few troubleshooting techniques. You will be introduced to the process of downloading Docker images and running them as containers. You'll learn how to run containers as a service (CaaS) and also discover how to share data among containers. Later on, you'll explore how to establish the link between containers and orchestrate containers using Docker Compose. You will also come across relevant details about application testing inside a container. You will discover how to debug a container using the docker exec command and the nsenter tool. Finally, you will learn how to secure your containers with SELinux and other proven methods.
Dynamical behaviour of the human β1-adrenoceptor under agonist binding

The human β1-adrenoceptor (hβ1AR) is a transmembrane (TM) protein responsible for the signal transduction pathway via agonist interaction. Despite its importance, hβ1AR activation mechanism is still unclear. The most studied and widely accepted mechanism is the disruption of a salt bridge between TM3 arginine and TM6 glutamic acid, called ionic lock. In this work, we constructed a functional hβ1AR-model equilibrated in a membrane environment to study the influence of agonist binding on the dynamical behaviour of hβ1AR and on the opening of the ionic lock. The results indicate that the agonist (R-noradrenaline) disturbs the hβ1AR, causing a TM helices rotation, disrupting the ionic lock. This rotational motion occurs in opposite directions in the intercellular and extracellular domains of hβ1AR, opening the ionic lock.
The transcription factor PITX1 drives astrocyte differentiation by regulating the SOX9 gene

Astrocytes perform multiple essential functions in the developing and mature brain, including regulation of synapse formation, control of neurotransmitter release and uptake, and maintenance of extracellular ion balance. As a result, astrocytes have been implicated in the progression of neurodegenerative disorders such as Alzheimer's disease, Huntington's disease, and Parkinson's disease. Despite these critical functions, the study of human astrocytes can be difficult because standard differentiation protocols are time-consuming and technically challenging, but a differentiation protocol recently developed in our laboratory enables the efficient derivation of astrocytes from human embryonic stem cells. We used this protocol along with microarrays, luciferase assays, electrophoretic mobility shift assays, and ChIP assays to explore the genes involved in astrocyte differentiation. We demonstrate that paired-like homeodomain transcription factor 1 (PITX1) is critical for astrocyte differentiation. PITX1 overexpression induced early differentiation of astrocytes, and its knockdown blocked astrocyte differentiation. PITX1 overexpression also increased and PITX1 knockdown decreased expression of sex-determining region Y box 9 (SOX9), known initiator of gliogenesis, during early astrocyte differentiation. Moreover, we determined that PITX1 activates the SOX9 promoter through a unique binding motif. Taken together, these findings indicate that PITX1 drives astrocyte differentiation by sustaining activation of the SOX9 promoter.
Hepatic Fat—Genetic Risk Score Predicts Hepatocellular Carcinoma in Patients With Cirrhotic HCV Treated With DAAs

Genetic factors and steatosis predispose to hepatocellular carcinoma (HCC) in patients with chronic hepatitis C virus; however, their impact in patients with cirrhosis cured by direct‐acting antivirals (DAAs) is still undefined. We assessed the association between a genetic risk score (GRS) of hepatic fat accumulation, combining variants in PNPLA3 (patatin‐like phospholipase domain containing 3), MBOAT7 (membrane bound O‐acyltransferase domain containing 7), TM6SF2 (transmembrane 6 superfamily member 2), GCKR (glucokinase regulator), and HCC in patients treated with DAAs.
RAP-011 Rescues the Disease Phenotype in a Cellular Model of Congenital Dyserythropoietic Anemia Type II by Inhibiting the SMAD2-3 Pathway

Congenital dyserythropoietic anemia type II (CDA II) is a hypo-productive anemia defined by ineffective erythropoiesis through maturation arrest of erythroid precursors. CDA II is an autosomal recessive disorder due to loss-of-function mutations in SEC23B. Currently, management of patients with CDA II is based on transfusions, splenectomy, or hematopoietic stem-cell transplantation. Several studies have highlighted benefits of ACE-011 (sotatercept) treatment of ineffective erythropoiesis, which acts as a ligand trap against growth differentiation factor (GDF)11. Herein, we show that GDF11 levels are increased in CDA II, which suggests sotatercept as a targeted therapy for treatment of these patients. Treatment of stable clones of SEC23B-silenced erythroleukemia K562 cells with the iron-containing porphyrin hemin plus GDF11 increased expression of pSMAD2 and reduced nuclear localization of the transcription factor GATA1, with subsequent reduced gene expression of erythroid differentiation markers. We demonstrate that treatment of these SEC23B-silenced K562 cells with RAP-011, a “murinized” ortholog of sotatercept, rescues the disease phenotype by restoring gene expression of erythroid markers through inhibition of the phosphorylated SMAD2 pathway. Our data also demonstrate the effect of RAP-011 treatment in reducing the expression of erythroferrone in vitro, thus suggesting a possible beneficial role of the use of sotatercept in the management of iron overload in patients with CDA II.
Normal regulatory alpha/beta T cells effectively eliminate abnormally activated T cells lacking the interleukin 2 receptor beta in vivo.

Although interleukin 2 (IL-2) has been thought to be the most important cytokine for T cell growth, animals lacking IL-2 or a component of its receptor molecules have more expanded T cells with activated memory phenotype, indicating an indispensable role for the IL-2/IL-2 receptor system in regulating the size and activity of the T cell population. In this study, we investigated the possible mechanism of abnormal expansion of activated T cells in IL-2 receptor β chain (IL-2Rβ)−/− mice using the systems of bone marrow transplantation and T cell transfer. Here, we show that IL-2Rβ2/− T cells in mice reconstituted with a mixture of IL-2Rβ2/− and IL-2Rβ1/+ bone marrow cells did not develop into an abnormally activated stage, and that already activated IL-2Rβ2/− T cells were effectively eliminated by IL-2Rβ1/+ T cells when both cells were cotransferred to T cell–deficient host mice. This regulation and/or elimination was dependent on T cells bearing α/β type T cell receptor, especially on CD8+ T cells and independent of the Fas–Fas ligand (FasL) system. IL-2Rβ1/+ T cells that eliminated activated IL-2Rβ2/− T cells expressed FasL, perforin, granzyme B, and tumor necrosis factor α/β. These results indicate a novel function of IL-2Rβ that is necessary for the induction of regulatory T cells acting to eliminate activated T cells.
Should public safety shift workers be allowed to nap while on duty?

Abstract Fatigue and sleep deficiency among public safety personnel are threats to wellness, public and personal safety, and workforce retention. Napping strategies may reduce work‐related fatigue, improve safety and health, yet in some public safety organizations it is discouraged or prohibited. Our aim with this commentary is to define intra‐shift napping, summarize arguments for and against it, and to outline potential applications of this important fatigue mitigation strategy supported by evidence. We focus our discussion on emergency medical services (EMS); a key component of the public safety system, which is comprised of police, fire, and EMS. The personnel who work in EMS stand to benefit from intra‐shift napping due to frequent use of extended duration shifts, a high prevalence of personnel working multiple jobs, and evidence showing that greater than half of EMS personnel report severe fatigue, poor sleep quality, inadequate inter‐shift recovery, and excessive daytime sleepiness. The benefits of intra‐shift napping include decreased sleepiness and fatigue, improved recovery between shifts, decreased anxiety, and reduced feelings of burnout. Intra‐shift napping also mitigates alterations in clinician blood pressure associated with disturbed sleep and shift work. The negative consequences of napping include negative public perception, acute performance deficits stemming from sleep inertia, and the potential costs associated with reduced performance. While there are valid arguments against intra‐shift napping, we believe that the available scientific evidence favors it as a key component of fatigue mitigation and workplace wellness. We further believe that these arguments extend beyond EMS to all sectors of public safety.
ON-FARM INNOVATION IN THE AUSTRALIAN WOOL INDUSTRY: A SENSEMAKING PERSPECTIVE

SUMMARY In agricultural innovation, the success of widely used technologies is often assumed to have been inevitable. Conversely, the blame for the failure of new technologies that researchers, policy makers and extensionists consider superior to existing solutions is often placed on farmers. However, these assumptions can be challenged by taking a social-constructivist view of on-farm innovation to examine how and why farmers made sense of new technologies and how this sensemaking shaped their use of these technologies over time. The present study took such an approach in its analysis of Australian woolgrowers’ adoption, abandonment, implementation and use of new wool-testing technologies that highlighted the social and dynamic nature of innovation on-farm. On-farm innovation in this case was an evolving, dynamic process that changed over time as woolgrowers made sense of new technologies. The primary message to agricultural innovation researchers, technology developers, policy makers and extensionists is that successful on-farm innovation requires the active, ongoing engagement of industry participants. In order to engage industry participants in the innovation process, sensemakers’ personal identity frames and social context, and how these interpretation frameworks relate to the new technology need to be understood.
The Development of Integumental Pore Signatures in the Genus Pleuromamma (Copepoda: Calanoida)

Most studies on the pore signature of calanoid copepods have been made on adult femalessemicolon very little is known of the ontogenetic development of the pore signatures and their sexual differences. Males, females and copepodids of five species in the metridinid genus Pleuromamma are examined. Interspecific differences in the pore signatures occur as early as copepodid III. Differences in the signatures of the copepodids arise from two sources: (1) species-specific rates of development of the adult signaturesemicolon (2) development of the species-specific components of the adult signature. The cephalosomal signature is complete in copepodid V, and the metasomal and urosomal signatures are complete in copepodid VI, the adult stage. Sexual differences in the pore signature are found in copepodid IV and are primarily evident in the urosome of the adult. The species-specific components of the urosomal signature are greater in the female than in the male.
Recurrent HyperCKemia with Immunological Involvement of the Endomysial Capillaries in Neuromyelitis Optica

A 55-year-old woman with neuromyelitis optica (NMO) had recurrent myalgias with hyperCKemia. A muscle biopsy suggested nonspecific myopathic changes. Regarding immunohistochemistry, the expression of both major histocompatibility complex class I and myxovirus resistance protein A was observed in the endomysial capillaries, suggesting immunological involvement of these capillaries, whereas both C5b9 (membrane attack complex) and aquaporin 4 immunofluorescence stainings were normal. The present findings led us to conclude that one possible mechanism for hyperCKemia in NMO underlying the immunological involvement of the endomysial capillaries was an as-yet-unidentified factor that triggered damage to the integrity of the sarcolemma and thereby cause CK leakage into the serum.
Estimation of aerial biomass of Lychnophora ericoides (Mart.)

For sustainable use of native plant species, knowledge of the amount of harvestable biomass is necessary. This study presents data on allometric relationships of Lychnophora ericoides Mart. (Asteraceae), an extractive resource in the Cerrado region of Brazil. On the Fazenda Agua Limpa (15o 45'S, 47o 57'W) of the Universidade de Brasilia, 38 individuals of this species were measured in the field, the parts above ground were harvested, separated into components and oven dried. The best regression equations to estimate biomass were geometric and the best fit was between total height and total biomass (r2 = 0.923). The economically useful portions, the leaves and branches accounted for approximately 20% of total above ground dry weight, but when used as the dependent variable, the strength of the relationship decreased (r2 = 0.694). The relationship between branch diameter and leaf biomass was similar to that between height and leaf dry weight (r2 = 0.600). The relation between the number of leaves and their biomass was linear but weak. The development of these equations is the first step towards the implementation of plans for sustainable use of this species.
15N isoscapes in a subtropical savanna parkland: spatial‐temporal perspectives

Spatial patterns of soil δ15N reflect variation in rates of N-cycling processes across landscapes. However, the manner in which soil δ15N is affected by vegetation and topoedaphic properties under non-steady state conditions is understood poorly. Here we propose and evaluate a conceptual model that explains how soil δ15N values will respond to changes in disturbance regimes (intensification of grazing and removal of fire) and the resultant invasion of a subtropical grassland by woody vegetation dominated by Prosopis glandulosa (honey mesquite), a N-fixing tree legume. Spatially-specific sampling along a catena (hill-slope) gradient where woody plants are known to have displaced grasses over the past 100 years revealed a positive relationship between soil δ15N and δ13C, and a negative relationship between NDVI and soil δ15N on upland portions of the landscape, indicating that plant cover is a critical determinant of δ15N spatial patterns. Because the dominant woody invader is a N-fixer, its invasion has in.
Chronic urticaria and dyspepsia: Association and treatment, an experimental study

Objective To determine the prevalence of Helicobacte pylori infection among chronic idiopathic urticaria patients and to assess their urticarial response after the eradication of H. pylori . Methods This study was a non-controlled, interventional study that was carried during the period from January 2016 to November 2016 at Dermatology Clinic, King Khalid Hospital, Najran, KSA. A total of 104 patients were included in our study. The patients who were positive for H. pylori were started on the eradication therapy. The effectiveness of the eradication therapy was assessed by repeating the H. pylori Urea Breath Test (UBT) after 4 weeks. Urticarial response was assessed 12 weeks after the eradication. Results The prevalence of chronic idiopathic urticaria (CIU) at our dermatology clinic was 0.6%. The patients were predominantly females (76.4%), and the highest number (69.4%) belonged to the age group ’20-50 years’. Of the study subjects (62.5%) had positive H. pylori , (91%) achieved eradication of H. Pylori while (9%) not. Of the cases (75.6%) showed significant improvement in their urticarial symptoms after eradication of H. pylori , while (24.4%) showed no significant improvement of their urticarial symptoms. Conclusion H. pylori should be specifically investigated for all chronic urticaria patients and the eradication therapy advised for those who have are positive for H. pylori .
Sex and mechanism of injury influence knee joint loading symmetry during gait 6 months after ACLR

Early‐onset knee osteoarthritis (OA) is associated with gait asymmetries after anterior cruciate ligament reconstruction (ACLR). Women have higher risks of sustaining non‐contact injuries, and are more likely to present with aberrant movement patterns associated with the mechanism of injury (MOI). We hypothesized that sex and MOI would influence gait after ACLR. Seventy participants, grouped by sex and MOI, completed biomechanical testing during over‐ground walking when they had full knee range of motion, trace or less knee effusion, greater than 80% quadriceps strength limb symmetry index, ability to hop on each leg without pain, and initiated running. Bilateral knee kinetics, kinematics, and joint contact forces were compared using mixed‐model analysis of variance (α = .05). There was a three‐way interaction effect of sex × MOI × limb for peak medial compartment contact force (P = .002), our primary outcome measure previously associated with OA development. Men with non‐contact injuries walked with asymmetry characterized by underloading of the involved limb. Men with contact injuries walked with the most symmetrical loading. In women, no clear pattern emerged based on MOI. Targeting, and possibly prioritizing interventions for athletes who present with gait asymmetries after ACLR based on sex and MOI, may be necessary to optimize outcomes. Statement of Clinical Significance: Sex and MOI may influence walking mechanics, and could be considered in future interventions to target gait symmetry, as a response to interventions may vary based on differences in sex and MOI.
Hearing Loss in Mucopolysaccharidoses: Current Knowledge and Future Directions

Mucopolysaccharidoses (MPS) are a group of lysosomal storage disorders caused by a deficiency of one of the enzymes involved in the degradation of glycosaminoglycans. Hearing loss is a common clinical presentation in MPS. This paper reviews the literature on hearing loss for each of the seven recognized subtypes of MPS. Hearing loss was found to be common in MPS I, II, III, IVA, VI, and VII, and absent from MPS IVB and MPS IX. MPS VI presents primarily with conductive hearing loss, while the other subtypes (MPS I, MPS II, MPS III, MPS IVA, and MPS VII) can present with any type of hearing loss (conductive, sensorineural, or mixed hearing loss). The sensorineural component develops as the disease progresses, but there is no consensus on the etiology of the sensorineural component. Enzyme replacement therapy (ERT) is the most common therapy utilized for MPS, but the effects of ERT on hearing function have been inconclusive. This review highlights a need for more comprehensive and multidisciplinary research on hearing function that includes behavioral testing, objective testing, and temporal bone imaging. This information would allow for better understanding of the progression and etiology of hearing loss. Owing to the prevalence of hearing loss in MPS, early diagnosis of hearing loss and annual comprehensive audiological evaluations are recommended.
A stable temperature may favour continuous reproduction by Theodoxus fluviatilis and explain its high densities in some karstic springs

Theodoxus fluviatilis is a common gastropod in many karstic springs in central Portugal. We investigated the possible reasons for the near-total restriction of this species to these springs. We first determined the spatial distribution of the species within a spring (Ancos) and related the densities at sampling-patch scales to selected physical and chemical variables. We then determined the densities at several locations downstream from the spring and related these densities to selected physical and chemical variables. Finally, we assessed the population dynamics of the gastropod in the spring. In the spring, T. fluviatilis was more abundant in shallow areas with a rapid current and cobble-boulder substrates. In June-July 2006, the mean densities of T. fluviatilis in the spring varied from ~ 10 to ~ 9000 individuals m–2 but decreased to zero 3800 m downstream. The physical and chemical changes along the stretch studied were minor; no significant correlations (Spearman rank correlation; p > 0.05) were observed between the gastropod abundances and the measured environmental variables or the PCA axes. In the spring, juveniles and egg masses were observed year-round, suggesting continuous reproduction. The temperature at the spring was fairly constant, ranging from 15.3 to 16.6 oC during the year (daily variation < 0.1 oC). Nine hundred metres farther downstream, the daily temperature variation reached 5 oC. We propose that the high densities of T. fluviatilis in the Ancos spring are maintained by continuous recruitment mediated by the constant high temperature.
Prevention of benign paroxysmal positional vertigo with vitamin D supplementation

Objective To assess the effect of vitamin D and calcium supplementation in preventing recurrences of benign paroxysmal positional vertigo (BPPV). Methods We performed an investigator-initiated, blinded-outcome assessor, parallel, multicenter, randomized controlled trial in 8 hospitals between December 2013 and May 2017. Patients with confirmed BPPV were randomly assigned to the intervention (n = 518) or the observation (n = 532) group after successful treatment with canalith repositioning maneuvers. The primary outcome was the annual recurrence rate (ARR). Patients in the intervention group had taken vitamin D 400 IU and 500 mg of calcium carbonate twice a day for 1 year when serum vitamin D level was lower than 20 ng/mL. Patients in the observation group were assigned to follow-ups without further vitamin D evaluation or supplementation. Results The intervention group showed a reduction in the ARR (0.83 [95% confidence interval (CI), 0.74–0.92] vs 1.10 [95% CI, 1.00–1.19] recurrences per 1 person-year) with an incidence rate ratio of 0.76 (95% CI, 0.66–0.87, p < 0.001) and an absolute rate ratio of −0.27 (−0.40 to −0.14) from intention-to-treat analysis. The number needed to treat was 3.70 (95% CI, 2.50–7.14). The proportion of patients with recurrence was also lower in the intervention than in the observation group (37.8 vs 46.7%, p = 0.005). Conclusions Supplementation of vitamin D and calcium may be considered in patients with frequent attacks of BPPV, especially when serum vitamin D is subnormal. Classification of evidence This study provides Class III evidence that for patients with BPPV, vitamin D and calcium supplementation reduces recurrences of BPPV.