Miracle-dz/UniFAR-FTUs huggingface dataset:
RNA recurrent motifs : identification and characterization

Although determination of the ribosome tertiary structure has been an outstanding step towards elucidation of the mechanism of protein synthesis, the complexity of this structure does not provide an easy answer of how this large molecular complex works. In order to understand the nature of structure-function relationships in the ribosome, the ribosome structure itself should be subjected to thorough analysis. In the last years, we undertook systematic efforts toward identification and characterization of all recurrent structural motifs existing in the ribosomal RNA and in other RNAcontaining molecules. The analysis of many instances of helix-helix packing in the ribosome structure allowed us to identify a new structural motif which we called “G-ribo”. In this motif, an interaction of the sugar edge of a guanosine in one helix with the ribose of a nucleotide from another helix was found to be at the origin of a complex network of concomitant inter-nucleotide interactions. In total, the G-ribo motif was found at eight locations within the ribosomal RNA. A surprising feature of this motif consists in its ability to favor the formation of pseudoknots of a particular type. In the ribosome structure, there are four pseudoknots whose formation is mediated by the G-ribo motif. Systematic analysis of the ribosome as well as the RNAseP crystal structures allowed for the identification of a new RNA motif, which we called “DTJ”, or DoubleTwist Joint motif. This motif is made of three short RNA double helices, which stack one on top of another. In the contact zone of each pair of helices two consecutive base pairs are over-twisted compared to the regular helical twist of 32° of A-RNA. One nucleotide of the base pair is always directly connected to the one nucleotide of the over-twisted base pair, while the opposite nucleotides of these base pairs are connected with one or several unpaired nucleotides. Introduction of the helical over-twist between two consecutive base pairs breaks the inter-nucleotide stacking and destabilizes the RNA double helix. In the DTJ, the unpaired nucleotides that connect the two over-twisted base pairs interact with one of the three motif-forming helices, providing an elegant strategy for the stabilization of the whole arrangement.
Studies on Bacterial Protease: Part XIV. Zinc as an Essential Metal Component of Neutral Protease of Bacillus subtilis var. amylosacchariticus

The neutral protease of Bacillus amylosacchariticus was inactivated by low concentrations of several metal-chelating agents and the inactivated enzyme with EDTA restored its activity almost completely by the addition of Zn++ or Co++ and partially by Fe++ or Mn++, if these metal ions were added shortly after the EDTA-treatment. The native enzyme was found to contain 0.19% of zinc together with a significant amount of calcium. Parallel increase in specific activity and zinc content of enzyme preparation was observed throughout the purification procedure. The elution pattern of enzyme activity on a CM-cellulose column chromatography also completely coincided with that of protein-bound zinc. A zinc-free inactive enzyme was also reactivated by the addition of zinc or cobalt ions, clearly showing that the neutral protease of B. amylosacchariticus is a zinc mctalloenzyme.
Potential and limitations of the low-cost SDS011 particle sensor for monitoring urban air quality

In Particulate Matter (PM) monitoring, current laser scattering low-cost sensor generations exhibit better stability than early sensor generations and feature internal digital processing to achieve more accurate results. As a representative of this class of sensors, we examine the popular SDS011 PM sensor. Previous work about co-location measurements between SDS011 sensors indicates that the sensor delivers adequate correlation under “typical” conditions, but performs less well under other ambient conditions, especially high humidity. To further explore the sensor's data quality in-depth, we conducted a series of controlled experiments with high precision reference devices and different aerosols, both polydisperse (ambient air, ammonium sulfate, soot) and monodisperse (polystyrene particles of different sizes). We also present results from a longer-term comparison (days) of multiple sensors, as well as the key influencing factors on uncertainty and assess the sensor’s potential and limitations. Our findings show that a single sensor generally does not capture PM10 satisfactorily and we discuss under which conditions PM2.5 readings reflect the ambient air quality adequately.
Atlas of fish histology

Our aim has been to present a general reference guide providing an extensive set of histological images of fishes (about 40 species). Althoughseveral studies treat histological aspects in relation to pathology, no recent synthesis on the normal histology of fish is available. We thereforebelieve this atlas will be a main contribution to this field. This atlas is designed for use by students and researchers, biologists, ichthyologists, fish farmers, veterinarians working in fisheries and, of course, by comparative histologists who want to learn more about the fish world.Guide general de reference sur l'histologie du poisson, destine a un large public disposant de quelques connaissances scientifiques de base ainsi que sur l'anatomie des poissons. Cet atlas peut donc s'adresser a des biologistes, histo(ichtyo)logistes et veterinaires en quete de renseignements precis, mais aussi a des etudiant(e)s et chercheurs travaillant dans les institutions academiques, ou encore a des aquariophiles, pisciculteurs et tout autre personne dont le « poisson » fait partie des centres d’interet. , chacun pouvant se focaliser sur les parties du livre qui repondront le mieux a leurs attentes et interrogations.Toutes les micrographies sont originales, issues de notre laboratoire (sauf indication) et en couleurs ; elles ont ete obtenues a partir d’une quarantaine d’especes et resultent exclusivement d'observations realisees a l'aide du microscope photonique. 450 photos d'une quarantaine d'especes
Curran's Atlas of Histopathology

Preface Methods 1. General Pathology 2. Blood, Spleen, Lymph Nodes and Bone Marrow 3. Ear, Nose and Mouth 4. Alimentary Tract 5. Live, Gall Bladder and Pancreas 6. Heart and Arteries 7. Trachea, Bronchi and Lungs 8. Endocrine Organs 9. Central Nervous System and Eye 10. Kidneys and Bladder 11. Male Generative System 12. Female generative System, including Breast 13. Bones, Cartilage and Joints 14. Skin Index
Cálculos cuánticos de resonancia magnética nuclear empleados en el desarrollo de una probabilidad para asignación estereoquímica

The characterization and structural elucidation of isolated complex organic molecules are a highly challenging task. Among the different spectroscopic techniques to carry out this purpose, the Nuclear Magnetic Resonance is the most useful one. Once the total connectivity of the molecule has been determined, the hardest job is to determine its 3D structure without stereochemical errors. Modern computational chemistry has contributed significantly to preventing these errors. In this context, we have developed some tools to interpret the correlation between experimental and calculated NMR data that contribute significantly to structural elucidation process. The DP4 probability is one of the most sophisticated and popular approaches for the stereochemical assignment of organic molecules using GIAO NMR chemical shift calculations when only one set of experimental data is available. In order to improve the performance of the method, we have developed a modified probability (DP4+), whose main differences from the original DP4 are the inclusion of unscaled data and the use of higher levels of theory for the NMR calculation procedure. With these modifications, a significant improvement in the overall performance was achieved providing accurate and confident results in establishing the stereochemistry of 48 challenging isomeric compounds.
Pressions sélectives dans les systèmes parasites-hôtes

Les pressions selectives dans les systemes parasites-hotes sont le resultat de l'opposition continuelle, a l'echelle du temps planetaire, des interets divergents de chacun des partenaires. Tandis que la fitness (« life-time reproductive success » des auteurs anglophones) du parasite est generalement augmentee par une plus grande frequence des rencontres avec l'hote et un meilleur taux de survie chez celui-ci, la fitness de l'hote est augmentee par des processus exactement inverses: diminution des rencontres et destruction du parasite. Ces processus, dits de courses aux armements, sont en accord avec l'hypothese de la Reine Rouge de Van Valen, qui suppose des changements adaptatifs continuels chez chacun des partenaires afin de repondre aux changements adaptatifs de l'autre. Les courses aux armements des systemes parasites-hotes suggerent donc une evolution de type gradualiste mais celle-ci ne s'oppose en rien aux idees actuelles sur les changements de tempo au cours de l'evolution biologique. De nombreux facteurs, soit genetiques (decalage adaptatif.), soit environnementaux (etat nutritionnel.), soit culturels (intervention vaccinale ou therapeutique) influencent le cours des maladies a l'echelle des individus. Le «terrain» individuel, qui est une composante du phenotype individuel, se trouve ainsi au carrefour des genes, de l'environnement et de la culture. Tout indique que les hommes doivent aujourd'hui compter davantage sur leur intelligence que sur la selection naturelle pour prevenir et guerir les maladies infectieuses et parasitaires.
Peripheral Arterial Chemoreceptors and Respiratory-Cardiovascular Integration

Chapter 1 - Introduction Chapter 2 - Morphology of the peripheral arterial chemoreceptors Chapter 3 - Nature of chemoreceptor stimuli and chemoreceptor responses Chapter 4 - Methods of eliciting reflex responses Chapter 5 - Discovery of the respiratory functions Chapter 6 - Reflex effects on cardiovascular system: role of changes in respiration Chapter 7 - Effects of respiration on the cardiovascular system Chapter 8 - Central integration of respiratory and autonomic functions Chapter 9 - Carotid bodies: primary cardiovascular responses Chapter 10 - Aortic bodies: primary cardiovascular responses Chapter 11 - Primary reflex effects on the pulmonary and bronchial circulations Chapter 12 - Respiratory mechanisms modulating cardiovascular responses of chemoreceptor origin Chapter 13 - Role of brain stem defence area Chapter 14 - Peripheral chemoreceptor functions in the fetus and neonate Chapter 15 - Interactions between arterial chemoreceptors and other inputs Chapter 16 - Some conditions involving the integrative control of the respiratory and cardiovascular systems Chapter 17 - Clinical implications of chemoreceptor reflexes Chapter 18 - General conclusions
Étude du contrôle de l’expression des systèmes de sécrétion de type III, généré par l’inactivation du gène yfgL codant une lipoprotéine de la membrane externe, chez Salmonella Enteritidis

Les salmonelles, responsables de fievres typhoides et gastro-enterites, sont un probleme majeur de sante publique. Les systemes de secretion de type III (TTSS) sont des facteurs de virulence cruciaux de Salmonella. Nos travaux ont montre que deletion du cadre ouvert de lecture yfgL conduit a une faible transcription des genes codant les 3 TTSS conduisant a une baisse importante de la virulence de Salmonella Enteritidis. Or, la deletion de ce gene, dont la proteine chez E.coli est en complexe avec les proteines YaeT, YfiO, NlpB et SmpA, conduit a une alteration de la membrane externe. Chez S. Enteritidis, nous avons montre que le role du complexe « YaeT » dans l’assemblage des proteines de membrane externe est conserve. Or, seule l’inactivation d’yfiO, conduit une diminution de l’expression des TTSS. Nos travaux ont toutefois suggere que le defaut d’assemblage des proteines de membrane externe n’est pas la cause de ce defaut d’expression des TTSS observes chez les mutants yfgL et yfiO.
OsDREB1 Gene from Rice Enhances Cold Tolerance in Tobacco

The OsDREB1 gene from rice encodes a transcription factor belonging to the DREBP transcription factor subfamily. Many DREBP transcription factors regulate gene expression in response to drought, high-salt, and cold stresses by binding specifically to the dehydration-responsive element (DRE). DRE-binding proteins, such as CBF1, DREB1A, and DREB2A, have been cloned from Arabidopsis thaliana and have been proved to play an important role in stress response of Arabidopsis and several other plants. In this study, the OsDREB1 gene was transferred to tobacco plants by the Agrobacterium-mediated transfer method, and 16 transgenic plants were identified. PCR analysis demonstrates that the foreign genes have been integrated into the tobacco genome. Results of freezing stress experiments indicate that the transgenic plants have enhanced cold tolerance.
An investigation into the transcriptional control of the Schwann cell during differentiation, demyelization and disease.

The generation of mature myelinating and non-myelinating Schwann cells from immature Schwann cells is regulated by signals from the axon. In the absence of axonal signals, for example during nerve injury, Schwann cells will return to an immature-like phenotype. However, Schwann cells retain the ability to re-differentiate after injury, demonstrating remarkable plasticity throughout adult life. Although these cellular transformations are normally beneficial, during diseases of the peripheral nervous system, they are often disrupted producing negative consequences. In this thesis, I have demonstrated a synergistic relationship between two signals, cyclic adenosine monophosphate (cyclic AMP) and p-neuregulin-1 (NRG1) in inducing myelin differentiation in cultured mouse Schwann cells. Furthermore, I found that the cyclic-AMP response element binding protein (CREB) family of transcription factors are required for this differentiation. Second, I have investigated the role of the transcription factor c-Jun in controlling Schwann cell responses after nerve injury. Using in vivo and in vitro approaches, I have found that c-Jun is an axonal-regulated injury factor that controls Schwann cell demyelination and dedifferentiation. Furthermore, Schwann cell derived c-Jun is crucial for adequate nerve regeneration and repair after injury. Finally, I investigated the functional properties of a mutant form of the my el in-related transcription factor Egr2 (Krox-20), which leads to severe congenital hypomyelinating neuropathy in humans. I found that mutant Egr2 is not transcriptionally inactive but retains residual wild-type Egr2 functions. More importantly, mutant Egr2 also demonstrates aberrant effects in Schwann cells, enhancing DNA synthesis, both in the presence and absence of the putative axonal mitogen, p-neuregulin 1. This is in stark contrast to wild-type Egr2, which causes withdrawal from the cell cycle.
Enzyme Systems that Metabolise Drugs and Other Xenobiotics

Contributors Forward Xenobiotic Metabolism: An Overview (C. Ionnides) Cytochrome P450 (F.P. Guengerich) Flavin Monooxygenases (J.R. Cashman) Amine Oxidases and the Metabolism of Xenobiotics (K.F. Tipton and S. Benedetti) Molybdenum Hydroxylases (C. Beedham) Prostaglandin Synthases (D.H. Degen, et al.) Lipoxygenases (A.P. Kulkarni) UDP-Glucuronosyltranferases (K.W. Bock) Glutathione S-transferases (P.J. Sherratt and J.D. Hayes) Sulphotransferases (H. Glatt) Arylamine Acetyltransferases (G.N. Levy and W.W. Weber) Mammalian Xenobiotic Epoxide Hydrolases (M. Arand and F. Oesch) Methyltransferases (C.R. Creveling) The Amino Acid Conjugations (G.B. Steventon and A.J. Hutt) Deconjugating Enzymes Sulphatases and Glucuronidases (C. Kunert-Keil et al.) Nitroreductases and Azoreductases (S. Zbaida) Index
Quality of Nursing Work Life, Work-Family Conflict, and Self-Regulation: A Structural Equation Modeling

The quality of nursing work life is an important index for assessing the professional health of nurses and providing tailored care services to patients. The study investigates the role of work-family conflict and selfregulation in predicting the quality of work-life among nurses. In this retrospective cross-sectional study, 230 nurses were selected randomly from public and private hospitals. They were asked to fill out Work-related Quality of Life Scale-2, Work–Family Conflict Scale, and Self-Regulation Questionnaire. Moreover, structural equation modeling was used to analyze the data by SPSS-19 and LISREL-8.80 software. The results showed that the model had a good fit to the observed data (RMSEA=0.06, GFI=0.93, AGFI=0.87, NFI=93, CFI=95, IFI=95, and P-value=.073) and the final model was verified. The results also revealed that “behavior-based and time-based work-family conflicts”, “assessing the plan’s effectiveness”, “searching for options”, and “implementing the plan” could significantly predict “stress at work”, “general well-being”, “working conditions”, employees’ engagement”, “job career satisfaction”, and “work-home interference” (p<0.05). It is indispensable to design tailored programs and professional health plans for improving family-work interference and self-regulatory actions, as two leading causes for the quality of nursing work life.
Dinámica poblacional de triatominos (Hemiptera- Reduviidae) relacionados con la transmisión de Trypanosoma cruzi en Paraguay, con énfasis en Triatoma sordida

El comportamiento biologico y el habitat de los triatominos son factores importantes en la epidemiologia de la enfermedad de Chagas. Los principales vectores colonizan las viviendas en altas densidades, presentan marcada antropofilia y elevada tasa de infeccion con Trypanosoma cruzi. Los vectores potenciales son catalogados como especies nativas o de “menor importancia epidemiologica” que ocupan habitats proximos al domicilio, son capaces de invadir las viviendas en bajas densidades e incapaces de colonizarlas en presencia de una especie primaria. Este manuscrito aporta informacion sobre la dinamica poblacional de los vectores potenciales involucrados con la transmision del T. cruzi, es decir aquellos triatominos que utilizan el peridomicilio y sus alrededores como area principal de interaccion entre hospederos domesticos, silvestres y sinantropicos, y aunque muchos de ellos no hayan colonizado las viviendas, muestran cambios en su comportamiento. Basados en la dinamica poblacional, estos cambios poblacionales estan relacionados con el patron de alimentacion y el flujo genico elevado que describe la invasion del domicilio en forma accidental y en algunas especies incluso se evidenciaron mecanismos adaptativos. Asi, este trabajo destaca la importancia de realizar estudios con enfoques eco-sistemicos aplicados a los vectores involucrados en la transmision de la tripanosomiasis, por lo cual existe la necesidad de incrementar investigaciones con vectores, capacidad de adaptacion, distribucion e indices de infeccion e infestacion de los mismos, lo cual conducira al diseno y la aplicacion de estrategias de control mas efectivas para disminuir la transmision del parasito.
CUSTOMER EXPERIENCE MANAGEMENT IN PUBLIC AND PRIVATE SECTOR BANKS – A STUDY

of the Study: Ÿ To analyse the Customer experience management practices in select Public and private sector banks. Ÿ To analyse the impact of CEM at the branch level, towards processing speed of the banking transactions. Ÿ To analyse the impact of CEM on public sector and private sector banking strategies. Ÿ To draw out conclusions and offer suggestions for more effective CEM in banks Methodology of the Study: A. Data Sources: The data was collected through both primary and secondary sources. The target respondents for this research included the customers of the selected branches of commercial banks i.e SBI & Organizations across the globe have seen Customer Experience Management (CEM) as a successor to the Customer Relationship Management (CRM). The current era of widespread technology enhanced the banks to adopt the new features and increased the level of customers to demand more. Indian banking sector in the post- demonetization facing a lot of challenges and found to be continuously involved in developing strategies for managing the experiences of customers. Banks introduced customer friendly technology in the process of developing relationship management systems and skills. The customer experience management in banks mainly focuses on providing potential level of proﬁciency in handling customers and delivering an amazing banking experience throughout the transactions performed by the customers. The banks in public and private sectors have been striving hard to attain the positive customer perception through effective experience management. This paper is an attempt to identify the customer perception towards customer experience management in State Bank of India (SBI) and Housing Development Finance Corporation (HDFC). ABSTRACT
Impact of ocular disease on circadian rhythms and brain connectivity

IV IMPACT STATEMENT . V ACKNOWLEDGEMENTS . VII STATEMENT OF COPYRIGHT . IX TABLE OF CONTENTS . X LIST OF FIGURES . XVII ABBREVIATIONS . XXII 1 GENERAL INTRODUCTION . 24 1.1 SLEEP . 25 1.2 CIRCADIAN PROCESS . 26 1.3 THE MAMMALIAN MOLECULAR CLOCK (TTFL) . 27 1.4 DISCOVERY OF CIRCADIAN RHYTHMS . 28 1.5 DISCOVERY OF THE PHOTOSENSITIVE RETINAL GANGLION CELLS (PRGCS) . 29 1.6 ABSORBANCE SPECTRA OF PHOTOPIGMENTS . 29 1.6. 1 THE SUPRACHIASMATIC NUCLEUS (SCN). 32 1.7 ANATOMY OF THE EYE . 33 1.7. 1 THE FUNCTIONAL ANATOMY OF THE RETINA . 34
Response of Weeds to Tillage and Cover Crop Residue

Total weed density increased after 1 yr of no-tillage and after 2 yr of conventional tillage in a 4-yr experiment with repeated assignment of the same treatment to the same plots. Large crabgrass, goosegrass, and carpetweed densities were higher in the no-tillage compared with the conventional-tillage treatment in at least 1 yr whereas common lambsquarters density was greater in the conventional-tillage treatment the last year of the experiment. Within the no-tillage treatment, rye or hairy vetch residue reduced total weed density an average of 78% compared to the treatment without cover crop when cover crop biomass exceeded 300 g m–2 and when residue covered more than 90% of the soil. Goosegrass, stinkgrass, and carpetweed densities were reduced by cover crop residue in at least 1 yr whereas large crabgrass was unaffected. Common lambsquarters density increased where rye was grown as a cover crop prior to conventional tillage. Despite differences in weed density among treatments, weed biomass was equivalent in all treatments during the last 2 yr.
A crossbreeding experiment to detect quantitative trait loci in dairy cattle.

INTRODUCTION In the last decade, advances in molecular genetics have made it possible to dissect the genetic variability of complex traits into quantitative trait loci (QTL). In France, two large programmes were carried out for QTL detection. The first one, in collaboration with the French artificial insemination industry, was a typical granddaughter design and used national records for milk production and health traits. This programme, now completed, intended to detect genes responsible for within breed variability, i.e. the variability available for selection (Boichard et al, 2002). The second one, carried out at the INRA experimental farm of Le Pin-Au-Haras (Normandy, France), and still running, has intended to detect genes responsible for the variability between the Holstein and Normande breeds. The purpose of the present paper was to describe its design, i.e. the current family structure obtained from specific reproduction and mating methods and the traits recorded. Finally, the expected QTL detection power, based on the current design, was estimated by simulation.