Miracle-dz/UniFAR-FTUs huggingface dataset:
Recent Advances in Self-Exciting Photodynamic Therapy

Photodynamic therapy (PDT) is already (Food and Drug Administration) FDA approved and used in the clinic for oncological treatment of pancreatic, lung, esophagus, bile duct, and of course several cancers of skin. It is an important tool in the oncological array of treatments, but for it exist several shortcomings, the most prominent of which is the shallow depth penetration of light within tissues. One-way researchers have attempted to circumvent this is through the creation of self-exciting “auto-PDT” nanoplatforms, which do not require the presence of an external light source to drive the PDT process. Instead, these platforms are driven either through oxidative chemical excitation in the form of chemiluminescence or radiological excitation from beta-emitting isotopes in the form of Cherenkov luminescence. In both, electronic excitations are generated and then transferred to the photosensitizer (PS) via Resonance Energy Transfer (RET) or Cherenkov Radiation Energy Transfer (CRET). Self-driven PDT has many components, so in this review, using contemporary examples from literature, we will breakdown the important concepts, strategies, and rationale behind the design of these self-propagating PDT nanoplatforms and critically review the aspects which make them successful and different from conventional PDT. Particular focus is given to the mechanisms of excitation and the different methods of transfer of excited electronic energy to the photosensitizer as well as the resulting therapeutic effect. The papers reviewed herein will be critiqued for their apparent therapeutic efficiency, and a basic rationale will be developed for what qualities are necessary to constitute an “effective” auto-PDT platform. This review will take a biomaterial engineering approach to the review of the auto-PDT platforms and the intended audience includes researchers in the field looking for a new perspective on PDT nanoplatforms as well as other material scientists and engineers looking to understand the mechanisms and relations between different parts of the complex “auto-PDT” system.
Topic Avoidance as a Privacy Management Strategy: Outcomes and Predictors of Parent Well-being and Sibling Caregiving Topic Avoidance

ABSTRACT As the population ages, siblings more frequently assume the role of filial caregiver. Siblings often avoid communication about caregiving, and this topic avoidance can be detrimental to well-being. Framed in the communication privacy management theory (CPM), the current study tests the associations between privacy rule criteria and siblings’ caregiving topic avoidance and the impact of topic avoidance on relationship satisfaction and depression. A sample of 415 caregivers, including 242 primary caregivers and 173 secondary caregivers, completed an online survey regarding their caregiving communication with a sibling. Topic avoidance about both parent well-being and sibling caregiving were positively associated with privacy rule criteria of parent health, liking, and risk-benefit ratio and negatively associated with trust. Parent well-being topic avoidance was associated positively with primary caregivers’ depression and negatively with relationship satisfaction for both primary and secondary caregivers. Sibling caregiving topic avoidance was associated positively with depression and negatively with relationship satisfaction for both primary and secondary caregivers.
Modelling Theta-Band Connectivity Between Occipital and Frontal Lobes: A Methodological MEG Study

Measuring functional connectivity between cortical regions of the human brain has become an important area of research. Modern theory suggests that brain networks exhibit non-stationarity, constantly forming and reforming depending on task demands. A robust means of determining effective connectivity in the short-lived neural responses that occur in event related paradigms would allow the investigation of event related cortico-cortical dynamics. We present such a mathematical model of wave propagation, motivated by current neuroscience literature, and demonstrate the utility of the method in a clinical sample of schizophrenia patients. MEG data were acquired in 10 patients with schizophrenia and 12 healthy controls during a relevance modulation task. Data were filtered into the theta band (4-8Hz) and source localised using a beamformer. The model was implemented using Fourier analysis methods which uncovered an event related travelling wave moving from the visual to frontal cortices. The model was validated using Monte Carlo phase randomisation and compared with another plausible model of wave propagation in the cortex. Results from the clinical sample revealed that wave speed was modulated by task condition and patients were found to have less difference between conditions (ANOVA revealing a significant interaction between group and condition, p<0.05). In conclusion, our method provides a simple and robust means to investigate event related cortico-cortical brain dynamics in individuals and groups in the task positive state.
Aziridinyl imines in organic synthesis: Development of tandem reaction strategies and application to total synthesis of natural products

Aziridinyl imines are well-known carbene equivalents because they are precursors of diazo compounds from which reactive intermediates can be produced. These carbene equivalents can be utilized as zwitterionic species, diradicals, or 4π system for cycloaddition reactions. Thus, the intermediates derived from aziridinyl imines have been used in the sulfur-ylide-mediated epoxide formation, tandem free-radical reactions, or cyclopropanation reaction via carbene intermediates to form trimethylenemethane (TMM) diyls, which undergo [2 + 3] cycloaddition reactions to form cyclopentanoids. Diazo compounds generated from aziridinyl imines also react with allenes to form TMM diyls. This reaction was utilized in tandem cycloaddition reactions of linear substrates to form polyquinanes. These tandem reaction strategies were successfully applied to the total synthesis of various cyclopentanoid natural products.
Lethargie, zivilgesellschaftlicher Aufruhr und Shrinking Spaces – Protest- und Bewegungsforschung in Ostmitteleuropa

Der Untergang kommunistischer Autokratien im östlichen Europa ist maßgeblich zivilgesellschaftlichen Akteur*innen zu verdanken: Dissident*innen hatten in den Jahren vor dem Fall des Eisernen Vorhangs eine Gegenöffentlichkeit und damit Räume für unabhängige politische Diskussionen geschaffen. So konnte frei von staatskommunistischer Einflussnahme über Menschenrechte und Systemreformen bzw. -alternativen debattiert werden. In der Konsequenz erodierte die Legitimität der kommunistischen Machthaber. Vertreter*innen der unabhängigen Zivilgesellschaft nutzen schließlich das durch die Perestroika aufgestoßene politische Gelegenheitsfenster und brachten die maroden Regime durch den Druck der Straße zu Fall – eine noch kurz zuvor unvorstellbare Entwicklung. Alles in allem wurde die bipolare Weltordnung somit im Wesentlichen von bewegten Bürger*innen überwunden – und dies (meist) ohne den Einsatz von Gewalt. Kein Wunder also, dass die Entwicklungen im östlichen Europa einen weltweiten Boom der Zivilgesellschaftsforschung auslösten (Klein 2001, 32). Als Schauplatz der Ereignisse erfüllte Ostmitteleuropa damit alle Voraussetzungen, um selbst zu einem Hotspot der Bewegungsforschung zu werden. Warum dies jedoch nicht eingetreten ist, wird in dem vorliegenden Artikel diskutiert. Dabei wird dargestellt, wie (1) die normative Aufladung der Zivilgesellschaft in den Jahren der Systemtransformation nach den Regimewechseln eine doppelte Enttäuschung bewirkte: Einerseits ebbte der Aktivismus im östlichen Europa schnell ab; andererseits zeigte sich, dass sich die wenigen aktiven Bürger*innen nicht zwangsläufig demokratiefördernd engagierten. Die der Zivilgesellschaft
Economic Mind: From Attribution Error to Self-Fulfilling Prophecy

Abstract I argue in this text that the economic mind is a culturally hegemonic, naturalistic interpretation of the behavior produced by the revolutionary nature of the economic and technical developments of the nineteenth and early twentieth centuries. Despite persistent criticism, people fulfilled the predictions of the economic model of a human being for so long that they committed an attribution error and took it to be the adequate vision of human nature. Neoclassical economic theory played a significant, even if involuntary, role in the spread of this illusion. I also claim that the concept of economic mind—as the dominant interpretation of human nature—currently functions as a self-fulfilling prophecy, reproducing behaviors that would have a chance to change (refuting the theory developed on their basis), were it not for the popularity of this concept as commonsensical definition of human nature.
Testate Amoeba Functional Traits and Their Use in Paleoecology

This review provides a synthesis of current knowledge on the morphological and functional traits of testate amoebae, a polyphyletic group of protists commonly used as proxies of past hydrological changes in paleoecological investigations from peatland, lake sediment and soil archives. A trait-based approach to understanding testate amoebae ecology and paleoecology has gained in popularity in recent years, with research showing that morphological characteristics provide complementary information to the commonly used environmental inferences based on testate amoeba (morpho-)species data. We provide a broad overview of testate amoeba morphological and functional traits and trait-environment relationships in the context of ecology, evolution, genetics, biogeography, and paleoecology. As examples we report upon previous ecological and paleoecological studies that used trait-based approaches, and describe key testate amoebae traits that can be used to improve the interpretation of environmental studies. We also highlight knowledge gaps and speculate on potential future directions for the application of trait-based approaches in testate amoeba research.
Modeling Catchment-Scale Nitrogen Losses Across a Land-Use Gradient in the Subtropics

Changing land use in subtropical and tropical catchments to farmland can result in higher nitrogen (N) loss to aquatic ecosystems. Here, we developed a lumped water and N balance model to estimate regional N losses to creeks at catchment scale within understudied subtropical catchments in Australia. The conceptual water balance model CoCa-RFSGD was extended by the nitrogen mass balance in top and subsoil by adding nitrogen cycle transformation estimates depending on meteorological, soil, and land-use properties. The model estimates the impact of pristine and agricultural land use on catchment-wide water quality using only low-order creek samples as water quality measurements of nitrate and nitrite (NOx) with increased model performance with increased agricultural coverage. The model revealed that an agricultural proportion of 3% in the study site drove a 3.5-fold increase of N losses to creeks and a 6.7-fold increase of N losses to the atmosphere compared to catchments without agriculture. Agricultural land use lost 92 kg-N ha−1, 85% of which evaded to the atmosphere and 15% was discharged via surface waters. A change from forest to cleared land may increase the total denitrification potential of a catchment. Overall, our lumped model provides a simple but effective tool to upscale local aquatic water quality measurements to the catchment scale, allowing for assessment of changing land use on aquatic N loads in areas with limited data availability.
Bombina variegata (Linnaeus, 1758) in rain pools in the Carpathian Mountains (Slovakia)

Abstract Rain pools (pluviotelmata or puddles) are common, small and non-permanent aquatic ecosystems. Data on the use of rain pools by amphibian taxa in the Carpathian Mountains, especially in Slovakia, are rare. In this study we describe the occurrence of Bombina variegata (Linnaeus, 1758), which may use these small aquatic ecosystems as part of their complex life cycle or where they spend their larval period. The most important hydroperiodic factors affecting the vertebrate fauna were the volume and area of the rain pools, the presence (respectively the absence) of shade, water temperature, biotope structure and the presence of several chemical compounds (NO−3, GH, KH, pH). This study confirms that micro-habitats, such as rain pools, may play an important role for suitable reproduction sites of B. variegata, as stated by several authors from the different countries and regions of its large distributional range. At the same time, data on the presence or absence of different taxa with important conservation status in the monitored habitat aid in the detection of species.
Comprehensive Genome-Wide Analysis of Thaumatin-Like Gene Family in Four Cotton Species and Functional Identification of GhTLP19 Involved in Regulating Tolerance to Verticillium dahlia and Drought

Thaumatin-like proteins (TLPs) present in the form of large multigene families play important roles in biotic stress and abiotic stress. However, there has been no systematic analysis of the TLPs in cotton. In this study, comprehensive identification and evolutionary analysis of TLPs in four species of cotton were conducted. In total, 50, 48, 91, and 90 homologous sequences were identified in Gossypium raimondii, G. arboreum, G. barbadense, and G. hirsutum, respectively. Gene structure, protein motifs, and gene expression were further investigated. Transcriptome and quantitative real-time PCR analysis indicated that GhTLPs participate in abiotic, biotic stress and cotton fiber development. GhTLP19 on chromosome At05 was selected as a candidate gene for further study. When GhTLP19 was silenced by virus-induced gene silencing (VIGS) in cotton, with the increase of malondialdehyde (MDA) content and the decrease of catalase (CAT) content, and as the increase of disease index (DI) and hyphae accumulation, the plants were more sensitive to drought and Verticillium dahliae. Furthermore, the GhTLP19 overexpressing Arabidopsis transgenic lines exhibited higher proline content, thicker and longer trichomes and more tolerance to drought when compared to wild type. This study will provide a basis and reference for future research on their roles in stress tolerance and fiber development.
Electrochemical Determination of Nicotine in Tobacco Products Based on Biosynthesized Gold Nanoparticles

In this work, gold nanoparticles were biosynthesized via Plectranthus amboinicus leaf extract as the reducing agent. A series of techniques were used for sample analysis. The biosynthesized gold nanoparticles (bAuNPs) are a uniform size with a spherical shape. The FTIR analysis reveals the presence of many oxygen-containing functional groups on the bAuNP surface. The cyclic voltammetry and electrochemical impedance spectroscopic characterizations reveal that while the bAuNPs have a slightly lower conductivity than chemically synthesized AuNPs (cAuNPs). However, the bAuNPs have a superior electrocatalytic performance toward nicotine reduction. After optimization, the bAuNP-modified SPE could detect nicotine linearly from 10 to 2,000 μM with a low detection limit of 2.33 μM. In addition, the bAuNPs/SPE have been successfully used for nicotine-containing-product analysis.
Understanding Behçet’s Disease in the Context of Innate Immunity Activation

Behçet´s disease (BD) is a heterogeneous condition consisting of idiopathic systemic vasculitis affecting large and small blood vessels of different types (i.e., arteries, veins, or capillaries). The disease frequently occurs in young adults without gender predilection, differently from several other autoimmune conditions. This challenging illness has recently been proposed by some authors as an example of complex autoinflammatory syndrome. Although much remains unanswered about BD pathogenesis, recent understanding of some aspects of innate immunity have clarified a few issues (and raised others). HLA-B*51 represents the strongest genetic risk factor for BD to date, albeit several other HLA-independent loci have also been associated with the disease. The consistent hyper-reactivity against Streptococcus sanguinis antigens and alterations in oral and gut microbioma suggests that infectious agents may play an important role. Moreover, functional abnormalities of pattern recognition receptors, especially Toll-like receptors in monocytes, have been demonstrated in patients with BD and can be associated with the development of the disease. Neutrophil hyperactivity is one of the most consistent findings in BD pathogenesis, as demonstrated by exacerbated constitutive oxidative burst, chemotaxis and NET formation. However, some studies suggest that the phagocyte-activated status in BD is not primary to the disease itself, but rather restricted to a fraction of patients with severe disease activity, and probably secondary to activating soluble factors carried by serum/plasma from BD patients. Herein we review the state of the art on BD etiopathogenesis with special emphasis on the participation of the innate immune system
A Vaccine Based on Kunitz-Type Molecule Confers Protection Against Fasciola hepatica Challenge by Inducing IFN-γ and Antibody Immune Responses Through IL-17A Production

Fasciola hepatica is helminth parasite found around the world that causes fasciolosis, a chronic disease affecting mainly cattle, sheep, and occasionally humans. Triclabendazole is the drug of choice to treat this parasite. However, the continuous use of this drug has led to the development of parasite resistance and, consequently, the limitation of its effectiveness. Hence, vaccination appears as an attractive option to develop. In this work, we evaluated the potential of F. hepatica Kunitz-type molecule (FhKTM) as an antigen formulated with a liquid crystal nanostructure formed by self-assembly of 6-O-ascorbyl palmitate ester (Coa-ASC16) and the synthetic oligodeoxynucleotide containing unmethylated cytosine-guanine motifs (CpG-ODN) during an experimental model of fasciolosis in mice, and we further dissected the immune response associated with host protection. Our results showed that immunization of mice with FhKTM/CpG-ODN/Coa-ASC16 induces protection against F. hepatica challenge by preventing liver damage and improving survival after F. hepatica infection. FhKTM/CpG-ODN/Coa-ASC16-immunized mice elicited potent IFN-γ and IL-17A with high levels of antigen-specific IgG1, IgG2a, and IgA serum antibodies. Strikingly, IL-17A blockade during infection decreased IgG2a and IgA antibody levels as well as IFN-γ production, leading to an increase in mortality of vaccinated mice. The present study highlights the potential of a new vaccine formulation to improve control and help the eradication of F. hepatica infection, with potential applications for natural hosts such as cattle and sheep.
Detection of Cerebrovascular Loss in the Normal Aging C57BL/6 Mouse Brain Using in vivo Contrast-Enhanced Magnetic Resonance Angiography

Microvascular rarefaction, or the decrease in vascular density, has been described in the cerebrovasculature of aging humans, rats, and, more recently, mice in the presence and absence of age-dependent diseases. Given the wide use of mice in modeling age-dependent human diseases of the cerebrovasculature, visualization, and quantification of the global murine cerebrovasculature is necessary for establishing the baseline changes that occur with aging. To provide in vivo whole-brain imaging of the cerebrovasculature in aging C57BL/6 mice longitudinally, contrast-enhanced magnetic resonance angiography (CE-MRA) was employed using a house-made gadolinium-bearing micellar blood pool agent. Enhancement in the vascular space permitted quantification of the detectable, or apparent, cerebral blood volume (aCBV), which was analyzed over 2 years of aging and compared to histological analysis of the cerebrovascular density. A significant loss in the aCBV was detected by CE-MRA over the aging period. Histological analysis via vessel-probing immunohistochemistry confirmed a significant loss in the cerebrovascular density over the same 2-year aging period, validating the CE-MRA findings. While these techniques use widely different methods of assessment and spatial resolutions, their comparable findings in detected vascular loss corroborate the growing body of literature describing vascular rarefaction aging. These findings suggest that such age-dependent changes can contribute to cerebrovascular and neurodegenerative diseases, which are modeled using wild-type and transgenic laboratory rodents.
Constructing dataset of classified drainage areas based on surface water-supply patterns in High Mountain Asia

ABSTRACT The High Mountain Asia (HMA) region, ranging from the Hindu Kush and Tien Shan in the west to the Himalaya in the south with an altitude between 2000 and 8844 m, holds the largest reservoir of glaciers and snow outside Earth Polar Regions. In the last decades, numerous glaciers and lake areas there have undergone tremendous changes with water redistribution. In order to increase understanding of the pattern of distribution of water resources, and their dynamic changes at the basin scale, a watershed classification based on the water replenishment patterns dataset was constructed. The input dataset are from the Randolph Glacier Inventory V.6. 0 and the vector data of rivers and streams. Four datasets were thus obtained: Glacier-fed and Runoff-fed Drainage Area (GRDA), Glacier-fed and Runoff-free Drainage Area (GDA), Glacier-free and Runoff-fed Drainage Area (RDA), and the Glacier-free and Runoff-free Drainage Area (NGRDA), and the numbers of these four types of basins are 87, 107, 32, and 448 separately. The statistical results show GRDA has the largest surface area, accounting for 82.2% of the total basin area in HMA, mainly in the region of the basin with outflow rivers or streams. Dominated by small basins, the GDA area accounts for the smallest area, only 3.86% and the RDA accounts for 5.62%. For NGRDA, most are with small areas, accounting for 8.32%, and mainly distributes in the closed basin of the Qiangtang Plateau. This dataset provides a fundamental classified data source for research on water resources, climate, ecology, and environment in HMA. The published data are available at https://data.4tu. nl/download/uuid:d07d748f-d10b-4308-9626-199ef05cc9af/ and http://www.dx. doi.org/10.11922/sciencedb.923.