Miracle-dz/UniFAR-FTUs huggingface dataset:
Renin Purification and Cloning

The 20 years we spent on the purification and cloning of renin contained secret difficulties and joys —it was difficult to contemplate sharing these with others. However, at the request of the editors of Hypertension, we finally agreed to relate the story of our research in this field to accompany the invited article by Tadashi Inagami in which he describes on the occasion of the death of one of the greatest researchers of renin, Erwin Haas, his own research on purification of renin and prorenin. Inagami, a biochemist experienced in the field of proteases, started his work in collaboration with Stanley Cohen, a Nobel Laureate in medicine, at Vanderbilt University in the 1970s. He and his colleagues, especially Kazuo Murakami, made major contributions to our knowledge of renin and still have the same tenacity and determination that we have in this research. Of course, our experience in the purification of renin was different from that of Inagami. Here is our story, from the purification to the cloning of renin, from our side of the Atlantic. For us, the purification of renin was the fruit of two minds, friendship, and team effort. We met as residents in medicine in the Department of Medicine run by P. Milliez in 1968 at the Broussais Hospital, Paris, and again in 1970 in the stimulating atmosphere of the National Institutes of Health (NIH) in the Reproduction Research Branch directed by M.B. Lipsett. We felt privileged to be associated with postdoctoral fellows from all over the world for whom the United States was providing high quality training and confidence in the importance of scientific and medical research. Having benefited from the teachings of F. Jacob and J. Monod at the Science Faculty in Paris, Pierre Corvol focused on proteins, their functions and the mechanism of their biosynthesis. His research project at NIH was the physicochemical characterization of sex hormone binding protein and the progesterone receptor. Joel M6nard, having measured the levels of rat renin by bioassay with Boucher and Genest, was trying to escape the tedium of this method by the development of radioimmunoassay with the expert help of Catt.
FACTORS AFFECTING MAINTENANCE OF QUALITY SERVICES IN PUBLIC INSTITUTIONS(A CASE STUDY OF WAJIR COUNTY REFERRAL HOSPITAL)

The study aimed at establishing factors affecting maintenance of quality services in public institutions, Wajir County Referral Hospital as the case study. The specific objectives of the study were; to find out the effect of work environment on maintenance of high quality services in Wajir County Referral Hospital, to determine the effect of staff capacity on the maintenance of high quality services in Wajir County Referral Hospital and to find out the influence or the effect of technology on maintenance of high quality services in Wajir County Referral Hospital. The study is of great significance to various stakeholders such as policy makers/planners, Ministry of Health and Sanitation professionals with new ideas on provision of quality health service to the public that should be taken into account in serving the public since quality, efficient and effective service provision offers an opportunity to a healthy and active nation. The independent variables of the study were work environment, staff capacity and technology, while the dependent variable will be maintenance of quality service. The target population of the study was divided into three: management team, medical staff and non-medical staff who add up to two hundred. 20% of the target population was selected to make up a sample size of 40. Radom sampling was used to select respondents for the sample size. Data was collected using a questionnaire having open and closed questions based on the objectives. Data was coded and analyzed using Microsoft Office Excel 2007 program to generate, bar charts, line graphs, pie charts, tables and histograms in the presentation of the data. The findings of the study showed that workers; lack adequate tools and equipment for service deliver, they work beyond the normal working and lack encouragement for service deliver. The hospital lacks adequate workforce therefore workers are overworked. The study revealed salary increments are not reflected in worker’s pay slip, overtime is not compensated and payments are delayed, these often results to industrial actions. The findings also revealed low use of technology in the hospital which led to slow service deliver lending to long queues. The study recommended provision of necessary tools and equipment’s for service deliver, hiring of more workforce, introduction of database technology, compensation for overtime, timely processing of payments and implementation of salary increment agreements.
Multiple-Input Multiple-Output Model Predictive Control of a Diesel Engine

Traditionally, diesel engine control has had to rely on indirect feedback variables and empirical open-loop maps because direct measurements of the variables representing highlevel objectives, such as emissions, have not been available in production engines. With new sensors being developed, the opportunity opens to design the controller directly based on highlevel objectives. In this paper, we propose to use model predictive control as a systematic way to go directly from high-level specifications to a control algorithm. The controller uses four actuator variables and five measured variables and is based on a model obtained through system identification. Experimental results on a six-cylinder heavy-duty engine around a fixed operating point demonstrate the potential of the control scheme. (Less)
A combinatorial method to enable detailed investigation of protein-protein interactions.

BACKGROUND Successful structural investigations of protein-protein interactions can be facilitated by studying only the core interacting regions of the constituent proteins. However, attempting the discovery of stable core complexes using informed trial-and-error approaches can prove time and resource intensive. METHODS We describe a valuable extension of combinatorial domain hunting (CDH), a technology for the timely elucidation of soluble protein truncations. The new method, CDH(2), enables empirical discovery of stable protein-protein core complexes. CDH(2) is demonstrated ab initio using a previously well-characterized Hsp90/Cdc37 complex. RESULTS Without using a priori information, we demonstrate the isolation of stable protein-protein complexes, suitable for further analyses. DISCUSSION This resource-efficient process can provide protein complexes for screening of compounds designed to modulate protein-protein interactions, thus facilitating novel drug discovery.
Level of Customer Satisfaction Towards Marketing Mix In Indonesian Traditional Market

The competition of various shopping centers in attracting consumers is getting more intense due to the increased consumer purchasing power. Many studies show that consumers are more interested to shop in a modern market, which has many attractive facilities, but only a few studies have examined the satisfaction of consumers shopping in traditional markets. The purpose of this study was to examine the level of customer satisfaction in traditional markets and to find out the priority strategies for improving traditional market performance. The data was analyzed using the Customer Satisfaction Index (CSI) and Importance Performance Analysis (IPA). Non-probability sampling was employed with an accidental sampling technique to as many as 180 customers in 8 traditional markets in Indonesia. Based on the research analysis, the CSI value obtained was 74.25%, showing that the customers were satisfied with the performance of traditional market merchants. The most considered marketing mix attribute to consumers in shopping was the friendliness of merchants when serving their consumers. This means that consumers did not only pay attention to the type of products purchased but also consider the services they received in the transaction. The priority strategy that needs to be done is to maintain the performance of attributes in Concentrate Here quadrant which consist of product availability, market hygiene, product price in line with expectations, lower product price, additional free product for certain quantity purchase, and discounted price from merchants.
The role of human leukocyte antigen class I polymorphism in HIV/AIDS

Purpose of reviewPolymorphism at human leukocyte antigen class I loci accounts for a portion of the individual variability in susceptibility to, and evolution of, HIV-1 infection. Evaluation of the impact of class I polymorphism has improved, with refined techniques for genotyping, quantifying peptide binding characteristics of class I molecules, and measuring cytotoxic T lymphocyte responses. Recent findingsMost class I alleles that are particularly advantageous or disadvantageous in controlling HIV/AIDS are human leukocyte antigen-B locus products. Some act more uniformly than others, across racial and viral subtype boundaries, and different alleles may exert their effects at different times during the course of infection. Disease progression may be retarded by alleles of less common supertypes. The virus mutates to escape host pressure exerted by class 1 programmed cytotoxic T lymphocytes, and may or may not revert to its earlier form, depending on the toll taken on its replication capacity. SummaryLarge virologically and immunologically well defined cohorts will be critical for increasingly comprehensive evaluation of the multiple small effects conferred by these highly polymorphic loci. Elucidating these effects at the population, clinical, cellular, and molecular levels poses a formidable challenge, but the reward could be vaccine constructs customized to the genetic profiles of individual populations, along with more effective individualized therapeutic intervention.
Prevalence of Tobacco Use among Young Adult Males in India: A Community-Based Epidemiological Study

Background: Prevalence of tobacco use in India is reaching alarming proportions, despite efforts by both World Health Organization (WHO) and Government of India (GOI) in controlling it. Part of the problem has been lack of available data on tobacco use in various groups. Although Global Youth Tobacco Survey (GYTS) and National Family Health Survey (NFHS) III have focused on adolescents and adults, respectively, data on use among young adults is lacking. Another limitation has been the use of the questionnaire method to determine tobacco use which may not reveal exact prevalence. This study aimed to explore the prevalence of tobacco use among young adult males in Ranchi, as confirmed by serum cotinine levels. Methods: Five-hundred male students were selected through systematic randomized process to represent 5 universities in Ranchi. After informed consent, the students were administered Tobacco and Other Substance Use questionnaire and then subjected to urine Rapid Nicotine Test to improve sensitivity and biologically confirm prevalence. All tobacco users then were administered Fagerstrom's Scale for Severity of Nicotine Dependence. Results and Conclusion: Biologically confirmed prevalence of tobacco use among male students was 55.6%, revealing high degree of prevalence in this age group. Predominant form of tobacco use was cigarettes (78%) followed by khaini (20%) and gutkha (2%), showing that most young adults use cigarettes possibly due to the ‘cool image’ associated with it. Seventy-seven percent of all tobacco users want to quit, thereby giving a strong opportunity to carry out cessation services in this group. There was higher mean Fagerstrom's Scale for Severity of Nicotine Dependence (FTND) score in smokers (6.7 ± 2.2) compared to chewers (4.6 ± 2.5), revealing higher severity of dependence among smokers than chewers.
Design of a Beam Reconfigurable THz Antenna With Graphene-Based Switchable High-Impedance Surface

In this paper, a new beam reconfigurable antenna is proposed for THz application, which is based on a switchable high-impedance surface (HIS) using a single-layer graphene. The effects of impurity density and gate voltage on the conductivity of graphene are utilized, and the switchable reflection characteristic of the graphene-based HIS is observed. Then the THz antenna is designed over this switchable HIS. By applying different voltages for different rows of HIS units, the antenna beam can be reconfigurable. The performance of the antenna is analyzed with its reflection coefficient, radiation pattern, and input impedance. The radiation beam of the antenna can vary in a range of ±30° as demonstrated by the simulated results.
The evaluation of the radioprotective effect of Triphala (an ayurvedic rejuvenating drug) in the mice exposed to γ-radiation

The effect of 0, 5, 6.25, 10, 12.5, 20, 25, 40, 50 and 80 mg/kg b. wt. of aqueous extract of triphala (an Ayurvedic herbal medicine) administrered intraperitoneally was studied on the radiation-induced mortality in mice exposed to 10 Gy of gamma-radiation. Treatment of mice with different doses of triphala consecutively for five days before irradiation delayed the onset of mortality and reduced the symptoms of radiation sickness when compared with the non-drug treated irradiated controls. The highest protection against GI (gastrointestinal) death was observed for 12.5 mg/kg triphala, where a highest number of survivors were reported up to 10 days post-irradiation. While 10 mg/kg triphala i.p. provided the best protection as evidenced by the highest number of survivors after 30 days post-irradiation in this group when compared with the other doses of triphala. Toxicity study showed that triphala was non-toxic up to a dose of 240 mg/kg, where no drug-induced mortality was observed. The LD50 dose i.p. of triphala was found to be 280 mg/kg b. wt. Our study demonstrates the ability of triphala as a good radioprotective agent and the optimum protective dose of triphala was 1/28 of its LD50 dose.
Efficacy of silver-coated fabric to prevent bacterial colonization and subsequent device-based biofilm formation.

Efficacy of silver-coated poly(ethylene terephthalate) to prevent bacterial attachment and subsequent infection was quantified in vitro, in both batch- and flowing-fluid experiments. Kinetic analysis of batch suspended cell cultures of Staphylococcus epidermidis (SE), at various growth-limiting nutrient concentrations, in the absence of any fabric, indicated a maximum culture growth rate constant micro(max) = 0.78 +/- 0.02 h(-1). Batch experiments for Control fabric samples indicated that SE cultures exhibited about the same suspended cell growth rate (0.72 +/- 0.02 h(-1)) as observed in batch suspended cultures without fabric. Suspended SE cultures in the presence of silver-coated fabric grew at a considerably lower rate, 0.15 +/- 0.01 h(-1), indicating the inhibitory effect of Ag(+2) ion released from the fabric. Growth rates of suspended SE cultures were 5-6 times higher in the fluid phase in contact with the Control fabric compared to cultures exposed to silver-coated fabric. Maximum suspended cell concentrations attained at time = 24 h were 1-2 orders of magnitude higher for Control fabrics vs. silver-coated fabric. In all batch colonization experiments, both live and dead SE bacterial cells accumulate on the surfaces of both silver-coated and Control fabrics. Adherent viable SE cells accumulated to 1-2 orders of magnitude more ( approximately 5 x 10(+8) cells/cm(2)) on Control fabric than SE cells on the silver-coated fabric ( approximately 1.1 x 10(+6) cells/cm(2)), respectively. Between 70-95% SE cells on the Control fabric were viable, while on the silver-coated fabric samples, at 24 h, viable cells were less than 10% of the adherent community (i.e., greater than 90% nonviable cells). In flow cell colonization experiments, SE cells accumulated on Control fabric to a maximum adherent cell concentration of 6 x 10(+7) - 8 x 10(+7) cells/cm(2) by 24 h with the proportion of viable cells remaining relatively constant at 76% throughout an experiment. Both noninvasive microscopic enumeration and destructive assays gave the same results for adherent cell numbers. Using silver-coated fabric, total cells numbers (live + dead) reached a level of approximately 1.1 x 10(+7) - 3.0 x 10(+7) cells/cm(2) after about 6 h and remained constant. However, while the proportion of viable cells initially on the surface was 63-75%, this fraction dropped continuously during each experiment to less than 6% viable cells at 24 h. Regardless of the criteria, the number of viable or nonviable cells attached to silver-coated fabric were significantly lower than on Control fabric.
Bullous pemphigoid—an adverse effect of ampicillin

A young patient developed an acute self‐limited bullous eruption shortly following ampicillin ingestion, with histopathological and immunofluorescent features fulfilling the criteria of bullous pemphigoid. A review of the relevant literature is made with special emphasis on the heterogenous and sometimes misleading faces of drug‐induced bullous pemphigoid.
The importance of internal fit, external fit, and employee cognitive frameworks in HRM systems

In this paper, we emphasize the importance of focusing on internal fit, external fit, and employee cognitive frameworks in HRM systems. We observe that cumulative research streams in the SHRM literature have not proven, or simply have assumed the existence of the synergetic effect of HRM practices, and that works examining the condition of its functioning have been few. We argue for the condition of synergetic effect of HRM practices, which has to incorporate ideas of internal fit, external fit, and the focus on employees’ cognitive mechanisms. We also argue that future research would be more fruit-ful with emphasis on examining the systems effect of HRM system of the firm.
Drivers of diverging financing conditions across Member States

The dispersion both of bank lending rates to the non-financial private sector and of overall financing costs for the corporate sector has increased considerably across the euro area throughout the recent crisis period. Such divergence has occurred despite the existence of a single monetary policy implemented through the provision of liquidity to the euro area banking sector under the principle of equal treatment. It does not seem to have been significantly affected by the ECB's new OMT programme. Available evidence suggests that greater cross-country variation in some structural characteristics of national banking systems, such as the quality of loan portfolios, profitability or the size of capital buffers, together with divergent financial positions of non-financial private sectors and sovereign funding costs, contributed to the increase in dispersion of bank lending rates at the country level.
Factors, Outcome, and the Solutions of Supply Chain Finance: Review and the Future Directions

In the current highly competitive and fast-changing business environment, in which the optimisation of all resources matters, creating an efficient supply chain is crucial. Earlier studies on supply chains have focussed on aligning product/services and information flows while neglecting the financial aspects. Due to this, in recent times, importance has been given to align financial flows with the other components of the supply chain. The interest in supply chain finance rose after the financial crisis when the bank loans declined considerably, as the need for better management and the optimisation of working capital became obvious. This paper reviews the articles on supply chain finance based on three themes—factors, outcomes, and solutions—while at the same time providing directions for future research on supply chain finance. This article is unique, as it investigates the factors affecting supply chains according to the existing literature. It also sheds light on the outcome of the supply chain without limiting the discussion only to the benefits. Further, it addresses the question: what are the solutions constituting supply chain finance?
Distributed scheduling of broadcasts in a radio network

A distributed algorithm is presented for obtaining an efficient and conflict-free broadcasting schedule in a multi-hop packet radio network. The inherent broadcast nature of the radio channel enables a node's transmission to be received by all other nodes within range. Multiple transmissions can be scheduled simultaneously because of the multi-hop nature of the network. It is first shown that the construction of a broadcasting schedule of minimum length is NP-complete, and then a centralized algorithm based on a sequential graph-coloring heuristic is presented to construct minimal-length schedules. A distributed implementation of this algorithm is then proposed, which is based on circulating a token through the nodes in the network. <<ETX>>
Pathophysiology of Dyspnea in COPD 147 Chemical and Mechanical Loads : What Have We Learned ?

Queen’s University, Kingston, Ontario, Canada; Harvard Medical School, Boston, Massachusetts; University of California at San Francisco School of Nursing, San Francisco, California; Los Angeles Biomedical Research Institute at Harbor–University of California at Los Angeles Medical Center, Torrance, California; University of Florida, Gainesville, Florida; Prince of Wales Medical Research Institute, Randwick, New South Wales, Australia; 7University of California at Los Angeles School of Medicine, Los Angeles, California; and 8Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire
Chronic Absenteeism in the Classroom Context: Effects on Achievement

Although educational policy makers uphold that chronic absenteeism (missing 10% or more of the school year) is damaging to students’ schooling outcomes, there is little empirical research to match. This study considers the role of spillover effects of chronic absenteeism on classmates’ achievement. It does so by utilizing a large-scale administrative urban district data set of elementary schoolchildren—a sample of students where the rates of chronic absenteeism are expected to be higher compared with the national average. The results show that students suffer academically from having chronically absent classmates—as exhibited across both reading and math testing outcomes. Chronic absenteeism not only had a damaging effect on those individuals missing excessive school days but also has the potential to reduce outcomes for others in the same educational setting.
Follicular lymphoma: 2018 update on diagnosis and management

Follicular lymphoma is generally an indolent B cell lymphoproliferative disorder of transformed follicular center B cells. Follicular lymphoma (FL) is characterized by diffuse lymphadenopathy, bone marrow involvement, splenomegaly and less commonly other extranodal sites of involvement. In general, cytopenias can occur but constitutional symptoms of fever, nightsweats, and weight loss are uncommon.