Miracle-dz/UniFAR-FTUs huggingface dataset:
Cell Treatment and Lysis in 96-Well Filter-Bottom Plates for Screening Bcr-Abl Activity and Inhibition in Whole-Cell Extracts

Although conventional high-throughput screens performed in vitro with purified protein kinases are powerful tools to discover new kinase inhibitors, they are far from ideal for determining efficacy in vivo. As a complementary approach, cell-based, target-driven secondary screens may help predict in vivo compound potency and specificity as well as evaluate bioavailability and toxicity. Here the authors report a simple protocol for treating K562 Bcr-Abl-expressing cells with small-molecule kinase inhibitors in 96-well filter-bottom plates followed by in-plate cell lysis. The lysates were assayed via a solid-phase kinase assay, allowing determination of apparent IC50 for known Bcr-Abl inhibitors as well as facilitating the screening of a small kinase inhibitor library. This approach may have further applications in generating lysates for analyzing kinase activity and inhibition in other nonadherent suspension cell lines.
Effects of Electrostatic Interactions on the Translocation of Polymers Through a Narrow Pore Under Different Solvent Conditions: A Dissipative Particle Dynamics Simulation Study

Polymer translocation through a narrow pore is investigated using a particle-based dissipative particle dynamics (DPD) method. A rigid core is included in each particle to avoid particle interpenetration problems based on the original DPD method. Electrostatic interactions of charged particles are simply represented via screened Coulombic interactions. The average translocation time τ versus polymer length N satisfies the scaling law τ ∼ Nβ. The scaling exponent β depends on solvent quality. The results demonstrate that solvent quality exerts a considerable influence on the dynamics of translocation of polymers. The findings may help facilitate understanding of the dynamic behaviors of various polymer and DNA molecules during translocation processes.
Collaborative and Overinvolved Parenting Differentially Predict Outcomes in Adolescents With Type 1 Diabetes.

OBJECTIVE The current study examined the impact of collaborative and intrusive parenting on depression and glycemic control in children with Type 1 diabetes (T1D). Research examining the association between parenting and child adjustment in this population has been limited by a reliance primarily on questionnaire data and cross-sectional analyses. To address these gaps, the current study used an observational coding system to measure the effects of parenting on child adjustment over a 1-year period. METHOD Youth (10 to 16 years old) with T1D and their mothers (N = 81) were recruited from an outpatient pediatrics diabetes clinic. Mothers' symptoms of anxiety and depression and children's depressive symptoms were assessed by self- reports; parenting behaviors were assessed via video-recorded observations coded using the Iowa Family Interaction Rating Scales; and adolescents' glycosylated hemoglobin (HbA1c) was obtained from medical records. RESULTS Bivariate correlations and linear regression analyses revealed that higher levels of observed collaborative parenting were related to significantly lower HbA1c 12 months later, and higher levels of observed overinvolved parenting were related to significantly greater child depressive symptoms 12 months later. Further, age and treatment type moderated the relation between overinvolved parenting and child depressive symptoms. CONCLUSIONS Collaborative and overinvolved parenting appears important for adolescents in predicting both psychological and health-related outcomes over time. Parenting behaviors may serve as an important target for future interventions to enhance adjustment in these children. (PsycINFO Database Record
Enterotoxin and toxic shock syndrome toxin‐one production by staphylococci isolated from mastitis in sheep

From 160 staphylococci isolated from ovine mastitis, 125 were identified as coagulase‐positive staphylococci (CPS) and 35 as coagulase‐negative staphylococci (CNS). Of these, 108 (87.8%) S. aureus produced at least one of the staphylococcal enterotoxins (SE) described. However, no CNS was found to be enterotoxigenic. Enterotoxin C (SEC) was the type most frequently produced. TSST‐1 was shown to be produced by 91 (74.0%) of S. aureus, almost invariably in combination with SEC. Three CNS strains were also found to produce TSST‐1 (two strains of S. xylosus and one strain of S. epidermidis). ;
APPLICATION OF TWIN FLASH STIMULI FOR SOME MACULAR DISEASES ISOLATION OF LOCAL RESPONSES BY TWIN FLASHES

Double flash ERG was examined for patients with visual loss from macular and optic nerve disorders, and the responses were compared. K values (= (B1‐B2))/B1 × 100) of the former were larger than normal values when the stimulus intervals were between 200 and 500 mseconds, and abnormality of K values seemed to correspond to the functional impairment of macula. On the other hand, K values of the patients with optic nerve diseases remained in the normal range. These facts indicate that double flash ERG is a very useful method to estimate the cone function clinically, and can be employed as a routine examination for patients with suspected cone dysfunction.
An 85-year-old woman with a history of falls.

Dr Delbanco: Mrs R is an 85-year-old widowed retired municipal worker who is the mother of 2 sons, one of whom is unmarried and lives with her in an urban community near Boston, Mass. Born in Italy, Mrs R came to the United States as a teenager and worked until retiring 10 years ago because of progressive arthritic pain and accompanying unsteadiness. She has no income other than Social Security, receives Medicare, and is eligible for lifesaving free medications at the Beth Israel Hospital, where she has been cared for by a general internist, Dr B, for more than 20 years. In recent years her medical problems have focused on difficulty walking and several falls and near falls, resulting in her being virtually homebound. She lives on the first floor of a house with a few steep steps outside the entrance, which limit her ability to move in and out
Comparison of angiogenic and anti-angiogenic factors in maternal and umbilical cord blood in early- and late-onset pre-eclampsia

Purpose: The aim of this study was to compare maternal and umbilical cord serum levels of the angiogenic and anti-angiogenic factors in early- and late-onset pre-eclamptic pregnancies as well as in normal pregnancies, which might have significant importance in the etiology of pre-eclampsia. Materials and Methods: This prospective case-control study was carried out with pre-eclamptic (early-onset, ≤ 34 weeks and late-onset, >34 weeks) and normal pregnant women. VEGF, PIGF, sFlt-1 and sEng levels in maternal and umbilical cord serum were measured before delivery and the findings were compared. Results: The study was conducted with 15 early- and 15 late-onset pre-eclampsia patients, and 17 patients with normal pregnancies. It was found that sEng levels were higher in the umbilical cord serum in the early-onset and in the maternal serum in the late-onset pre-eclampsia group than the control group (p < 0.05). No significant difference in any factor was observed between the early- and late-onset pre-eclampsia groups. Conclusion: In this study, the results showed that angiogenic and anti-angiogenic factor levels in maternal serum and umbilical cord serum may not be related to the time of onset of pre-eclampsia.
A 10Gb/s Backplane decision feedback equalizer in 90nm-CMOS technology

This paper presents the design of a 10-Gb/s decision feedback equalizer (DFE) for chip-to-chip communications in 90-nm CMOS technology. A fast slicer architecture is proposed that achieves timing constraints without the use of speculation techniques. Removing speculation improves power consumption by 60%. The 5-tap topology was found optimum to compensate for channel losses up to 22 dB. A half-rate architecture is used to enable operation at 10-Gb/s. The DFE system consumes 43 mW from a 1.2 V supply, and occupies an area of 177µm ∗ 146µm. Post-layout simulations done using a channel with 22dB loss at 5 GHz, demonstrate the effectiveness of the DFE equalization.
Stereoscopic Depth Perception by Static Stereo- Deficient Observers in Dynamic Displays with Constant and Changing Disparity

The performance of 11 static stereo-deficient subjects and 11 static stereo-normal subjects was compared on two types of dynamic stereo displays-one where disparities were constant during motion and one where disparities changed continuously. Computer-generated displays simulating horizontal motion of figures at different depths or rotation of figures about a vertical axis were viewed through a Brewster stereoscope. About one-half of the subjects in our static stereo-deficient sample were able to make depth judgments on the basis of disparity in both types of dynamic displays. The clinical feature which appeared to distinguish those static stereo-deficient subjects who could use disparity information in dynamic displays from those who could not was early onset constant strabismus. These results indicate that a complete evaluation of stereo ability should include tests with dynamic displays, possibly including both constant and changing disparities.
Wissensbasierte Konfiguration von Transferzentren

Kurzfassung Zielstellung des Forschungsprojekts ist die Entwicklung eines Software-Agentensystems zur Planung der Rekonfiguration von Transferzentren. Die äßerst produktiven Transferzentren sind konzeptbedingt rekonfigurierbar. Durch die sehr hohe Komplexität wird diese Möglichkeit jedoch nicht zur Effektivitätssteigerung in der Produktion genützt. Eine agentenbasierte Fertigungssystemplanung kann diese Komplexität beherrschen. Zur Konzeption der Software wird hierzu ein Organisationsmodell entwickelt.
Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans

An enterotoxin-producing strain of Escherichia coli isolated from a case of cholera-like diarrhea (E. coli strain H-10407) was found to possess a surface-associated colonization factor. Colonization was manifested as the ability of small inocula (10(5) bacteria) to attain large (10(9)) populations in the infant rabbit intestine with a concomitant diarrheal response. A laboratory-passed derivative of E. coli H-10407, designated H-10407-P, failed to exhibit an increase in population in the infant rabbit and also failed to induce diarrhea. Cell-free culture supernatant fluids of E. coli H-10407 and H-10407-P produced equivalent enterotoxic responses in infant and in adult rabbits. Specific anti-colonization factor antiserum was produced by adsorbing hyperimmune anti-H-10407 serum with both heat-killed and living cells E. coli H-10407-P. This specific adsorbed serum protected infant rabbits from challenge with living E. coli H-10407 although the serum did not possess bactericidal activity. The anti-colonization factor serum did not agglutinate a strain of E. coli K-12 possessing the K88 colonization factor peculiar to E. coli enterotoxigenic for swine. By electron microscopy it was demonstrated that E. coli H-10407, but not H10407-, possessed pilus-like surface structures which agglutinated with the specific adsorbed (anti-colonization factor) antiserum. E. coli H-10407 possessed three species of plasmid deoxyribonucleic acid, measuring 60 X 10(6), 42 X 10(6), and 3.7 X 10(6) daltons, respectively. E. coli H-10407-P possessed only the 42 X 10(6)- and the 3.7 X 10(6)-dalton plasmid species. Spontaneous loss of the specific H-10407 surface-associated antigen was accompanied by loss of the 60 X 10(6)-dalton species of plasmid deoxyribonucleic acid and loss of colonizing ability. Thus, it is concluded that the E. coli colonization factor described here is a virulence factor which may play an important and possibly essential role in naturally occurring E. coli enterotoxic diarrhea in man.
Dual-side cooled novel IPM and improved capability of inverter for elevated-temperature operations

Novel two-side cooling for integrated power modules (IPM) is developed for elevated-temperature operations. This approach applies and improves commercially off-the-shelf (COTS) products of IPM without significantly changing the original packaging design and manufacture's fabrication process. It can reduce the p-n junction temperature rise of the power devices inside by 20% at an equivalent load, thus increasing operating ambient temperatures, which is desirable for integrated motors and elevated-temperature applications. The size and volume associated with conventional cooling mechanism can be shrunk. DC-link capacitor reduction with adaptive PWM control is also discussed
Prevalence of Obesity, Overweight, and Underweight in Qatari Adolescents

Background Overweight and obesity have reached epidemic proportions in Arabian oil-rich countries and are threatening to become a global epidemic. Excess weight has a great impact on the health and quality of life of individuals. However, prevalence of underweight in the developing world has shown a decline during the last decade. Objective The aim of this cross-sectional study was to determine the prevalence of underweight, overweight, and obesity, as measured by body-mass index, in a representative sample of adolescents aged 12 to 17 years in the State of Qatar. Methods Qatari schoolchildren (n = 3,923) from 30 schools in urban and semiurban districts responded to a sociodemographic questionnaire. Body-mass index was calculated and the prevalence of overweight and obesity was determined on the basis of cutoff points of the International Obesity Task Force (IOTF) values (above the 85th and 95th percentiles, respectively, for overweight and obesity). Underweight was defined on the basis of CDC (Centers for Disease Control and Prevention) less than 5th percentile of BMI for age. Results The final sample consisted of 1,968 boys and 1,955 girls. The prevalence of underweight, overweight, and obesity was 8.6%, 28.6%, and 7.9%, respectively, among adolescent boys and 5.8%, 18.9%, and 4.7% among girls. The prevalence of underweight was highest at 16 years of age among boys (10.5%) and at 17 years among girls (8.9%). The prevalence of obesity was highest at 12 years of age among boys (11.7%) and at 13 years among girls (6.4%). The 95th percentile curve for boys was above the IOTF standard curve; the 95th percentile curve for girls was below the IOTF curve. Conclusions Adolescents living in the State of Qatar are at high risk for overweight and obesity. There is a need to establish a national program for the prevention and treatment of obesity and related complications.
Changing Moods: How Manual Tracking by Family Caregivers Improves Caring and Family Communication

Previous research on healthcare technologies has shown how health tracking promotes desired behavior changes and effective health management. However, little is known about how the family caregivers' use of tracking technologies impacts the patient-caregiver relationship in the home. In this paper, we explore how health-tracking technologies could be designed to support family caregivers cope better with a depressed family member. Based on an interview study, we designed a simple tracking tool called Family Mood and Care Tracker (FMCT) and deployed it for six weeks in the homes of 14 family caregivers who were caring for a depressed family member. FMCT is a tracking tool designed specifically for family caregivers to record their caregiving activities and patient's conditions. Our findings demonstrate how caregivers used it to better understand the illness and cope with depressed family members. We also show how our tool improves family communication, despite the initial concerns about patient-caregiver conflicts.
Dopamine: a novel antitumor agent active against B-16 melanoma in vivo.

L-dopa methyl ester has been shown to be an effective antitumor agent against the B-16 melanoma in vivo. We have now examined the analog, dopamine, a major catabolite of L-dopa. Dopamine administration at a daily dose of 600 mg/kg resulted in a 48% (p less than .001) increase in survival of treated mice as compared to non-treated controls. In vitro, an effect similar to that observed with L-dopa methyl ester was noted, specifically, a rapid and profound inhibition of thymidine incorporation with little effect on uridine or leucine incorporation. We have postulated that the inhibition of a DNA polymerase might be the site of action of these novel antitumor agents.
miR-27 b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH 11 and epithelial-mesenchymal transition

Dysregulation of microRNAs (miRNAs) occurs frequently in cervical carcinogenesis. miRNAs function as tumor-suppressors or oncogenes and are involved in tumor behavior. However, the expression and function of miR-27b in cervical carcinogenesis remain unknown. In the present study, we observed that miR-27b was significantly increased in cervical cancer cells and tissues, and upregulation of miR-27b was negatively associated with its direct target, cadherin 11 (CDH11). Upregulation of miR-27b significantly accelerated the proliferation, cell cycle transition from G1 to S phase, migration and invasion of C33A cells, while downregulation of miR-27b suppressed the proliferation and invasion of HeLa cells. Moreover, CDH11 cDNA transfection impaired the oncogenic effect of miR-27b on cancer cells. Knockdown of CDH11 attenuated the suppressive effect of an miR-27b inhibitor on cervical cancer cells. In addition, we found that CDH11 was involved in miR-27b-induced epithelial-mesenchymal transition (EMT) by regulating expression of E-cadherin, vimentin and N-cadherin. Our results for the first time indicate that miR-27b acting as an oncogene may play an important role in the progression of cervical cancer by modulating CDH11 and EMT.
Pregnancy‐related pharmacokinetic changes

The pharmacokinetics of many drugs are altered by pregnancy. Drug distribution and protein binding are changed by pregnancy. While some drug metabolizing enzymes have an apparent increase in activity, others have an apparent decrease in activity. Not only is drug metabolism affected by pregnancy, but renal filtration is also increased. In addition, pregnancy alters the apparent activities of multiple drug transporters resulting in changes in the net renal secretion of drugs.
Vers l'échantillonnage d'un entrepôt de données

Résumé. L'afflux de données sur les usages des produits et services nécessite des traitements lourds pour les transformer en information. Or la capacité à traiter les données ne peut pas suivre l'augmentation exponentielle des volumes stockés. Avec les technologies actuelles, un difficile compromis doit être trouvé entre le coût de mise en œuvre et la qualité de l'information produite. Nous proposons une approche basée sur l'échantillonnage d'un entrepôt de données pour déployer à moindre coût un système d'information décisionnel utilisant tout notre potentiel d'information. La brique technologique essentielle pour construire ce système repose sur un opérateur d'échantillonnage des jointures.
İŞLETMELERDE BİLGİ TEKNOLOJİLERİ (BT) YETENEKLERİ KAVRAMI, ÖNEMİ VE BT YETENEKLERİN GELİŞTİRİLMESİ ÜZERİNDE ROL OYNAYAN FAKTÖRLER

The aim of this paper is to explain the concept of Information Technologies (IT) capability and its importance for today`s companies in addition to revealing the possible antecedents that take part in developing IT capability in the context of resource based view. In order to do so, we study the effects of antecedents which are Technologic Orientation, Resource Commitment, and Managerial Involvement, that we considered as the antedecends, on the Technical IT capability and Human Based IT Capability which represent the two dimensions of the core IT competence.The research is conducted on the 60 IT personel of 17 firms operating in manufacturing and service industry, located around Kocaeli, Istanbul and Sakarya at 2016. The data is tested, using Cronbach’s alpha analysis, factor analysis and corelation and regession analysis. The findings provide empirical evidence in support of the relationships amongs resource commitment, managerial involvement and technical IT capability; thus all the the antecedents are found to be positively associated with developing IT capabilities.
Defense Mechanisms of Pregnant Mothers Predict Attachment Security, Social-Emotional Competence, and Behavior Problems in Their Toddlers.

OBJECTIVE For at-risk (single parent, low income, low support) mothers, healthy adaptation and the ability to manage stress have clear implications for parenting and the social-emotional well-being of their young offspring. The purpose of this longitudinal study was to examine associations between defense mechanisms in pregnant women and their toddlers' attachment security, social-emotional, and behavioral adjustment. METHOD Participants were 84 pregnant women during their last trimester of pregnancy, recruited from community agencies primarily serving low-income families. Women were followed prospectively from pregnancy through 2 years after birth and completed several multimethod assessments during that period. Observations of mother-child interactions were also coded after the postnatal visits. RESULTS Multiple regression analyses revealed that mothers' defense mechanisms were significantly associated with several toddler outcomes. Mature, healthy defenses were significantly associated with greater toddler attachment security and social-emotional competence and fewer behavior problems, and less mature defenses (disavowal in particular) were associated with lower levels of attachment security and social-emotional competence. Associations remained significant, or were only slightly attenuated, after controlling for demographic variables and partner abuse during pregnancy. CONCLUSIONS The study findings suggest that defensive functioning in parents preparing for and parenting toddlers influences the parent-child attachment relationship and social-emotional adjustment in the earliest years of life. Possible mechanisms for these associations may include parental attunement and mentalization, as well as specific caregiving behavior toward the child. Defensive functioning during times of increased stress (such as the prenatal to postnatal period) may be especially important for understanding parental influences on the child.
The Apparent Turnover of 1-Aminocyclopropane-1-Carboxylate Synthase in Tomato Cells Is Regulated by Protein Phosphorylation and Dephosphorylation

In suspension-cultured cells of tomato (Lycopersicon esculentum Mill.), the activity of 1-aminocyclopropane-1-carboxylate synthase (ACC-S) rapidly increases in response to fungal elicitors. The effect of inhibitors of protein kinases and protein phosphatases on the regulation of ACC-S was studied. K-252a, an inhibitor of protein kinases, prevented induction of the enzyme by elicitors and promoted its apparent turnover in elicitor-stimulated cells, causing a 50% loss of activity within 4 to 8 min in both the presence and absence of cycloheximide. Calyculin A, an inhibitor of protein phosphatases, caused a rapid increase of ACC-S in the absence of elicitors and an immediate acceleration of the rate of ACC-S increase in elicitor-stimulated cells. In the presence of cycloheximide there was no such increase, indicating that the effect depended on protein synthesis. Cordycepin, an inhibitor of mRNA synthesis, did not prevent the elicitor-induced increase in ACC-S activity but strongly reduced the K-252a-induced decay and the calyculin A-induced increase of its activity. In vitro, ACC-S activity was not affected by K-252a and calyculin A or by treatments with protein phosphatases. These results suggest that protein phosphorylation/dephosphorylation is involved in the regulation of ACC-S, not by regulating the catalytic activity itself but by controlling the rate of turnover of the enzyme.