Miracle-dz/UniFAR-FTUs huggingface dataset:
Estudo-piloto: células NK nas gestantes com LES

The innate immune system plays an important role in reproduction, with marked involvement of NK cells. These cells behavior during pregnancy may clarify crucial points in the pathogenesis of complications that may occur in pregnant women with SLE. OBJECTIVE: To measure the amount of circulating NK cells and their viability in pregnant SLE patients. MATERIALS AND METHODS: Blood samples from four groups of ten patients each were evaluated: 1. GLES: Pregnant SLE patients; 2. PLES: Non-pregnant SLE patients; 3. Gcontrols: Pregnant controls; 4. Controls: Healthy non-pregnant women. In all patients the amount and viability of NK cells was measured by flow cytometry, as well as the total apoptosis by annexin V and propidium iodite staining. RESULTS: Due to the great variability, median of each group was used for evaluation: CD56+ count [GLES (0.10), PLES (0.12), Gcontrols (0.15), Controls (0.08)]; total apoptosis (addition of initial and late apoptosis to total number of dead cells) [GLES (0.06), PLES (0.04), Gcontrols (0.11), Controls (0.11)]. The results for live cells count had low variability, so the averages and standard deviations were used for comparisons: [GLES (0.91±0.06), PLES (0.95±0.03), Gcontrols (0.86±0.11), Controls (0.88+0.08)]. CONCLUSION: Although not statistically significant, the total apoptosis in the SLE groups was lower than in the control groups, and the live cell count was higher. This suggests that in SLE patients, pregnant or not, the NK cells have a prolonged life cycle (or have a lower/different turnover), and that there may be a higher immune stimulus making the NK cells take longer to activate the apoptosis process.
E-Cigarettes

Electronic cigarettes (e-cigarettes) are products that deliver a nicotine-containing aerosol (commonly called vapor) to users by heating a solution typically made up of propylene glycol or glycerol (glycerin), nicotine, and flavoring agents (Figure 1) invented in their current form by Chinese pharmacist Hon Lik in the early 2000s.1 The US patent application describes the e-cigarette device as “an electronic atomization cigarette that functions as substitutes [sic] for quitting smoking and cigarette substitutes ” (patent No. 8,490,628 B2). By 2013, the major multinational tobacco companies had entered the e-cigarette market. E-cigarettes are marketed via television, the Internet, and print advertisements (that often feature celebrities)2 as healthier alternatives to tobacco smoking, as useful for quitting smoking and reducing cigarette consumption, and as a way to circumvent smoke-free laws by enabling users to “smoke anywhere. ”3 Figure 1. Examples of different electronic cigarette (e-cigarette) products. Reproduced from Grana et al.1 There has been rapid market penetration of e-cigarettes despite many unanswered questions about their safety, efficacy for harm reduction and cessation, and total impact on public health. E-cigarette products are changing quickly, and many of the findings from studies of older products may not be relevant to the assessment of newer products that could be safer and more effective as nicotine delivery devices. In addition, marketing and other environmental influences may vary from country to country, so patterns of use and the ultimate impact on public health may differ. The individual risks and benefits and the total impact of these products occur in the context of the widespread and continuing availability of conventional cigarettes and other tobacco products, with high levels of dual use of e-cigarettes and conventional cigarettes at the same time among adults4–8 and youth.9–11 It is important to assess e-cigarette toxicant exposure and …
IMMUNOLOGY OF EXPERIMENTAL TUMORS.

One of the most important advances in cancer biology of recent years is the conclusive demonstration that a variety of experimental tumors possess antigenicity capable of eliciting specific immune responses. This review will be restricted largely to those experimental systems in which the antigenic differences between tumor and host have been most clearly defined. In prac­ tice, this limits the field of discussion, with certain exceptions, to studies per­ formed with tumors of recent origin from inbred rodent stocks. A discussion of immunological studies with human tumors is not possible within the com­ pass of this review; this area has recently been reviewed by Southam (1). However, consideration will be given to those aspects of experimental tumor immunology that have direct implications for the study of tumors in man. Other topics of interest which cannot be dealt with here include the use of immunological techniques to identify the products of plasma cell and other neoplasms (2, 3), the immunological aspects of choriocarcinoma in man (4), and immunological evidence concerning the loss of normal cell constituents during the malignant transformation [see Gorer (5»).
Multivariate analysis of risk factors for neck metastases in surgically treated parotid carcinomas.

OBJECTIVE To analyze risk factors for neck metastases in patients with parotid carcinomas. DESIGN Cohort of patients followed up from 1 to 366.2 months at a single institution. SETTING Referral center, private or institutional practice, hospitalized care. PATIENTS A total of 145 patients with parotid carcinomas with complete clinical and pathological information. The histological diagnosis was reviewed according to the World Health Organization classification for salivary gland tumors. INTERVENTION Patients were treated by surgery alone (62 cases) or with postoperative radiotherapy (83 cases). A neck dissection was performed in 80 patients. MAIN OUTCOME MEASURE Rates of neck lymph node metastasis. Univariate and multivariate analyses were carried out using logistic regression evaluating the significance of demographic, clinical, and pathological data. RESULTS The following variables were significantly associated to the risk of lymph node metastasis by univariate analysis: histological type (P<.001), T stage (P<.001), desmoplasia (P = .001), facial palsy (P = .02), perineural invasion (P = .01), extraparotid tumor extension (P = .02), and necrosis (P = .003). By multivariate analysis, histological type (P<.001), T stage (P = .03), and desmoplasia (P = .006) had the highest correlation with lymph node metastasis. CONCLUSION The significant risk factors for neck metastasis in parotid carcinoma were histological type (ie, adenocarcinoma, undifferentiated carcinoma, high-grade mucoepidermoid carcinoma, squamous cell carcinoma, and salivary duct carcinoma), T stage (T3 and T4), and desmoplasia (severe).
Free voice and pure tone audiometer for routine testing of auditory acuity; studies on comparative efficiency.

THE RELATION between a free voice acuity test and a pure tone audiogram is a complex one of many factors; no final statement has yet been made by which the interpretation of either can be made in terms of the other or by which both can be reduced to a common denominator. Some laboratories depend exclusively on a pure tone audiogram as a measure of how well a man can hear speech, while others look askance on the pure tone test and claim that only a test using speech sounds is valid as a measure of speech reception. An early discussion of the relation between speech reception and audiometry is that of Fletcher, 1 who compared a free voice test and audiometry through the speech range (512 to 2048 cycles per second). Fletcher concluded, from the rather close correspondence of hearing loss, that the free voice and the pure tone audiogram
Contribution of Community Forestry in Poverty Reduction: Case Study of Multiple Community Forests of Bajhang District, Nepal

Abstract: In Nepal, nearly half of the total land is covered by forest, which holds a potentially important position in promoting rural livelihoods and in alleviating rural poverty. The rural landscape that encompasses an agrarian economy, a fragile ecology, and a complex and differentiated society is changing rapidly in Nepal today. Although poverty alleviation has been one of the top priorities for national development since 1976, poverty still remains widespread, persistent and it is also an acute problem in Nepal, where people are in a state of deprivation with regard to incomes, clothing, housing, healthcare, education, sanitary facilities and human rights. Thus, Nepal is considered as one of the poorest countries in South-Asia, with 25.2% people living below the poverty line. The objective of this study was to assess changes in poverty of forest users brought on by the community forestry program, in order to analyze the level of participation in community forestry management activities. For this study, Bajhang district was chosen as the study site, which is one of the poorest and most remote districts in the country of Nepal. Different Participatory Rural Appraisal methods such as face-to face interviews, focus group discussions and key informants' interviews including secondary data were used to gather information. The findings showed that the forest users' participation in meetings, discussion and other activities, like community forestry management or silvicultural operation related to community forestry, was high. The assessment found that 42.3%, 32.6% and 25.1% of respondents strongly agreed, agreed and were neutral, respectively, towards the idea that poverty reduction from community forests had occurred. The results showed almost all the respondents were depended upon agriculture and/or forest resources for their livelihoods. Different ecosystem services such as ethnomedicines, aesthetic value and ecotourism, control of soil erosion/land-slides, water recharge and soil fertility have increased due to the decomposition of leaf litter. This was apparent from the formulation of community forests. Poverty in rural areas of the country is still higher than in urban areas and the incidence of poverty is the highest in the Far western Province where this research was conducted, Therefore, the government, policy makers and other stakeholders should work hand-in-hand to effectively reduce the poverty that persists in Nepal.
Examining the Potential of Developing and Implementing Use of Adiponectin-Targeted Therapeutics for Metabolic and Cardiovascular Diseases

Cardiometabolic diseases encompass those affecting the heart and vasculature as well as other metabolic problems, such as insulin resistance, diabetes, and non-alcoholic fatty liver disease. These diseases tend to have common risk factors, one of which is impaired adiponectin action. This may be due to reduced bioavailability of the hormone or resistance to its effects on target tissues. A strong negative correlation between adiponectin levels and cardiometabolic diseases has been well-documented and research shown that adiponectin has cardioprotective, insulin sensitizing and direct beneficial metabolic effects. Thus, therapeutic approaches to enhance adiponectin action are widely considered to be desirable. The complexity of adiponectin structure and function has so far made progress in this area less than ideal. In this article we will review the effects and mechanism of action of adiponectin on cardiometabolic tissues, identify scenarios where enhancing adiponectin action would be of clinical value and finally discuss approaches via which this can be achieved.
Friend, Foe or Both? Immune Activity in Alzheimer’s Disease

Alzheimer’s disease (AD) is marked by the presence of amyloid beta (Aβ) plaques, neurofibrillary tangles (NFT), neuronal death and synaptic loss, and inflammation in the brain. AD research has, in large part, been dedicated to the understanding of Aβ and NFT deposition as well as to the pharmacological reduction of these hallmarks. However, recent GWAS data indicates neuroinflammation plays a critical role in AD development, thereby redirecting research efforts toward unveiling the complexities of AD-associated neuroinflammation. It is clear that the innate immune system is intimately associated with AD progression, however, the specific roles of glia and neuroinflammation in AD pathology remain to be described. Moreover, inflammatory processes have largely been painted as detrimental to AD pathology, when in fact, many immune mechanisms such as phagocytosis aid in the reduction of AD pathologies. In this review, we aim to outline the delicate balance between the beneficial and detrimental aspects of immune activation in AD as a more thorough understanding of these processes is critical to development of effective therapeutics for AD.
Non-immune Hydrops Fetalis and Hepatic Dysfunction in a Preterm Infant With Congenital Syphilis

We report a case of a preterm infant with congenital syphilis who presented with non-immune hydrops fetalis. Hepatic dysfunction was present at birth and acutely worsened following antibiotic administration. Placental pathology demonstrated infiltration with numerous spirochetes. Although critically ill, the infant recovered with intravenous penicillin G and supportive care. This case demonstrates that congenital syphilis remains a contemporary disease demanding enhanced awareness from clinicians. Manifestations evident in utero or in the newborn can be severe and may result in fetal demise or neonatal death. Moreover, we hypothesize that the treatment resulted in a Jarisch-Herxheimer reaction as manifested by the hepatic deterioration. The incidence of congenital syphilis and its associated complications can be greatly reduced with strict adherence to universal prenatal testing and comprehensive follow-up.
Interferon-Independent Innate Responses to Cytomegalovirus

The critical role of interferons (IFNs) in mediating the innate immune response to cytomegalovirus (CMV) infection is well established. However, in recent years the functional importance of the IFN-independent antiviral response has become clearer. IFN-independent, IFN regulatory factor 3 (IRF3)-dependent interferon-stimulated gene (ISG) regulation in the context of CMV infection was first documented 20 years ago. Since then several IFN-independent, IRF3-dependent ISGs have been characterized and found to be among the most influential in the innate response to CMV. These include virus inhibitory protein, endoplasmic reticulum-associated IFN-inducible (viperin), ISG15, members of the interferon inducible protein with tetratricopeptide repeats (IFIT) family, interferon-inducible transmembrane (IFITM) proteins and myxovirus resistance proteins A and B (MxA, MxB). IRF3-independent, IFN-independent activation of canonically IFN-dependent signaling pathways has also been documented, such as IFN-independent biphasic activation of signal transducer and activator of transcription 1 (STAT1) during infection of monocytes, differential roles of mitochondrial and peroxisomal mitochondrial antiviral-signaling protein (MAVS), and the ability of human CMV (HCMV) immediate early protein 1 (IE1) protein to reroute IL-6 signaling and activation of STAT1 and its associated ISGs. This review examines the role of identified IFN-independent ISGs in the antiviral response to CMV and describes pathways of IFN-independent innate immune response induction by CMV.
Technology Advancements in Blood Coagulation Measurements for Point-of-Care Diagnostic Testing

In recent years, blood coagulation monitoring has become crucial to diagnosing causes of hemorrhages, developing anticoagulant drugs, assessing bleeding risk in extensive surgery procedures and dialysis, and investigating the efficacy of hemostatic therapies. In this regard, advanced technologies such as microfluidics, fluorescent microscopy, electrochemical sensing, photoacoustic detection, and micro/nano electromechanical systems (MEMS/NEMS) have been employed to develop highly accurate, robust, and cost-effective point of care (POC) devices. These devices measure electrochemical, optical, and mechanical parameters of clotting blood. Which can be correlated to light transmission/scattering, electrical impedance, and viscoelastic properties. In this regard, this paper discusses the working principles of blood coagulation monitoring, physical and sensing parameters in different technologies. In addition, we discussed the recent progress in developing nanomaterials for blood coagulation detection and treatments which opens up new area of controlling and monitoring of coagulation at the same time in the future. Moreover, commercial products, future trends/challenges in blood coagulation monitoring including novel anticoagulant therapies, multiplexed sensing platforms, and the application of artificial intelligence in diagnosis and monitoring have been included.
Interleukin-7 Receptor Alpha in Innate Lymphoid Cells: More Than a Marker

Innate lymphoid cells (ILCs) are a group of immune cells that are important for defense against pathogens, tissue repair, and lymphoid organogenesis. They share similar characteristics with various subsets of helper T cells but lack specific antigen receptors. Interleukin-7 (IL-7) and thymic stromal lymphopoietin (TSLP) are cytokines that engage the IL-7Rα and have major roles in dictating the fate of ILCs. Recent advances in the field have revealed transcriptional programs associated with ILC development and function. In this article, we will review recent studies of the role of IL-7 and TSLP in ILC development and function during infection and inflammation.
Genetic Background Influences the Propagation of Tau Pathology in Transgenic Rodent Models of Tauopathy

Alzheimer’s disease (AD), the most common tauopathy, is an age-dependent, progressive neurodegenerative disease. Epidemiological studies implicate the role of genetic background in the onset and progression of AD. Despite mutations in familial AD, several risk factors have been implicated in sporadic AD, of which the onset is unknown. In AD, there is a sequential and hierarchical spread of tau pathology to other brain areas. Studies have strived to understand the factors that influence this characteristic spread. Using transgenic rat models with different genetic backgrounds, we reported that the genetic background may influence the manifestation of neurofibrillary pathology. In this study we investigated whether genetic background has an influence in the spread of tau pathology, using hippocampal inoculations of insoluble tau from AD brains in rodent models of tauopathy with either a spontaneously hypertensive (SHR72) or Wistar-Kyoto (WKY72) genetic background. We observed that insoluble tau from human AD induced AT8-positive neurofibrillary structures in the hippocampus of both lines. However, there was no significant difference in the amount of neurofibrillary structures, but the extent of spread was prominent in the W72 line. On the other hand, we observed significantly higher levels of AT8-positive structures in the parietal and frontal cortical areas in W72 when compared to SHR72. Interestingly, we also observed that the microglia in these brain areas in W72 were predominantly phagocytic in morphology (62.4% in parietal and 47.3% in frontal), while in SHR72 the microglia were either reactive or ramified (67.2% in parietal and 84.7% in frontal). The microglia in the hippocampus and occipital cortex in both lines were reactive or ramified structures. Factors such as gender or age are not responsible for the differences observed in these animals. Put together, our results, for the first time, show that the immune response modulating genetic variability is one of the factors that influences the propagation of tau neurofibrillary pathology.
A HARDWARE IMPLEMENTATION OF PETRI NETS MODELS

This paper presents the structure of a reconfigurable FPGA system for Petri Net simulation. The system is described as the interactions between processed elements that are functionally defined. According to the interconnection mode of the processed elements Petri Net models with any complexity can be implemented. The processed elements have a flexible architecture that allows their adaptation to the simulation of Petri Net model. The processed elements architecture and their interconnection mode gives the possibility to reduce the execution time that is necessary for reachability analysis of Petri Net.
Blind normalization of public high-throughput databases

The rise of high-throughput technologies in the domain of molecular and cell biology, as well as medicine, has generated an unprecedented amount of quantitative high-dimensional data. Public databases at present make a wealth of this data available, but appropriate normalization is critical for meaningful analyses integrating different experiments and technologies. Without such normalization, meta-analyses can be difficult to perform and the potential to address shortcomings in experimental designs, such as inadequate replicates or controls with public data, is limited. Because of a lack of quantitative standards and insufficient annotation, large scale normalization across entire databases is currently limited to approaches that demand ad hoc assumptions about noise sources and the biological signal. By leveraging detectable redundancies in public databases, such as related samples and features, we show that blind normalization without constraints on noise sources and the biological signal is possible. The inherent recovery of confounding factors is formulated in the theoretical framework of compressed sensing and employs efficient optimization on manifolds. As public databases increase in size and offer more detectable redundancies, the proposed approach is able to scale to more complex confounding factors. In addition, the approach accounts for missing values and can incorporate spike-in controls. Our work presents a systematic approach to the blind normalization of public high-throughput databases.
Communications of the Association for Information Systems Communications of the Association for Information Systems

: In this paper, we present the report from a professional development workshop (PDW) that addressed questions at the intersection of digital innovation and digital transformation at ICIS 2019 in Munich, Germany. The PDW focused on sharing insights into 1) the current state of research on digital innovation, 2) the current state of research on digital transformation, and 3) the methodological and theoretical challenges in conducting research on digital innovation and/or digital transformation. Accordingly, the PDW featured three keynotes on digital innovation and digital transformation as a basis for interactive roundtable discussions among authors about their ongoing research projects. Across the three keynotes and the roundtable discussions, some common patterns emerged. In particular, reoccurring themes included the challenge of balancing new and old elements of organization and technology in digital innovation and transformation and methodological challenges related to empirical research design and theory choice. In this paper, we synthesize ideas that we developed prior to, during, and after the PDW. We also present some suggestions for future research.