Miracle-dz/UniFAR-FTUs huggingface dataset:
Dynamic Localization of the Human Papillomavirus Type 11 Origin Binding Protein E2 through Mitosis While in Association with the Spindle Apparatus

ABSTRACT Papillomaviral DNA replicates as extrachromosomal plasmids in squamous epithelium. Viral DNA must segregate equitably into daughter cells to persist in dividing basal/parabasal cells. We have previously reported that the viral origin binding protein E2 of human papillomavirus types 11 (HPV-11), 16, and 18 colocalized with the mitotic spindles. In this study, we show the localization of the HPV-11 E2 protein to be dynamic. It colocalized with the mitotic spindles during prophase and metaphase. At anaphase, it began to migrate to the central spindle microtubules, where it remained through telophase and cytokinesis. It was additionally observed in the midbody at cytokinesis. A peptide spanning residues 285 to 308 in the carboxyl-terminal domain of HPV-11 E2 (E2C) is necessary and sufficient to confer localization on the mitotic spindles. This region is conserved in HPV-11, -16, and -18 and bovine papillomavirus type 4 (BPV-4) E2 and is also required for the respective E2C to colocalize with the mitotic spindles. The E2 protein of bovine papillomavirus type 1 is tethered to the mitotic chromosomes via the cellular protein Brd4. However, the HPV-11 E2 protein did not associate with Brd4 during mitosis. Lastly, a chimeric BPV-1 E2C containing the spindle localization domain from HPV-11 E2C gained the ability to localize to the mitotic spindles, whereas the reciprocal chimera lost the ability. We conclude that this region of HPV E2C is critical for localization with the mitotic apparatus, enabling the HPV DNA to sustain persistent infections.
Semiparametric Approach to a Random Effects Quantile Regression Model

We consider a random effects quantile regression analysis of clustered data and propose a semiparametric approach using empirical likelihood. The random regression coefficients are assumed independent with a common mean, following parametrically specified distributions. The common mean corresponds to the population-average effects of explanatory variables on the conditional quantile of interest, whereas the random coefficients represent cluster-specific deviations in the covariate effects. We formulate the estimation of the random coefficients as an estimating equations problem and use empirical likelihood to incorporate the parametric likelihood of the random coefficients. A likelihood-like statistical criterion function is proposed that, which we show is asymptotically concave in a neighborhood of the true parameter value and motivates its maximizer as a natural estimator. We use Markov chain Monte Carlo samplers in the Bayesian framework, and propose the resulting quasi-posterior mean as an estimator. We show that the proposed estimator of the population-level parameter is asymptotically normal, and that the estimators of the random coefficients are shrunk toward the population-level parameter in the first-order asymptotic sense. These asymptotic results do not require Gaussian random effects, and the empirical likelihood-based likelihood-like criterion function is free of parameters related to the error densities. This makes the proposed approach both flexible and computationally simple. We illustrate the methodology with two real data examples.
Alarm Trend Catcher: Projecting Operating Conditions in the Electrical Power Grid Domain with Interactive Alarm Visualization

Electric power grid operation is a critical activity in which control room operators supervise the status of the grid infrastructure in order to avoid undesirable situations. One of the most important tasks of this operation is projecting ahead the infrastructure state in real time. This task is currently based on managing alarms, with the responsibility of the control room operator to estimate both their relevance and evolution in time. A review of traditional alarm visualization formats highlights some deficiencies, which may lead to operating inefficiencies or even critical operating problems. Driven by a field study, this paper presents Alarm Trend Catcher, an innovative alarm visualization approach aimed at assisting not only the perception of upcoming alarms but also the projection of future states of the electric power grid. The user study of this visualization approach shows supporting evidence of its benefits for gaining interesting operational insights from the alarm information.
Effects of the Infusion of 4% or 20% Human Serum Albumin on the Skeletal Muscle Microcirculation in Endotoxemic Rats

Background Sepsis-induced microcirculatory alterations contribute to tissue hypoxia and organ dysfunction. In addition to its plasma volume expanding activity, human serum albumin (HSA) has anti-oxidant and anti-inflammatory properties and may have a protective role in the microcirculation during sepsis. The concentration of HSA infused may influence these effects. We compared the microcirculatory effects of the infusion of 4% and 20% HSA in an experimental model of sepsis. Methods Adult male Wistar rats were equipped with arterial and venous catheters and received an intravenous infusion of lipopolysaccharide (LPS, serotype O127:B8, 10 mg/kg over 30 minutes) or vehicle (SHAM, n = 6). Two hours later, endotoxemic animals were randomized to receive 10 mL/kg of either 4% HSA (LPS+4%HSA, n = 6), 20% HSA (LPS+20%HSA, n = 6) or 0.9% NaCl (LPS+0.9%NaCl, n = 6). No fluids were given to an additional 6 animals (LPS). Vessel density and perfusion were assessed in the skeletal muscle microcirculation with sidestream dark field videomicroscopy at baseline (t0), 2 hours after LPS injection (t1), after HSA infusion (t2) and 1 hour later (t3). The mean arterial pressure (MAP) and heart rate were recorded. Serum endothelin-1 was measured at t2. Results MAP was stable over time in all groups. The microcirculatory parameters were significantly altered in endotoxemic animals at t1. The infusion of both 4% and 20% HSA similarly increased the perfused vessel density and blood flow velocity and decreased the flow heterogeneity to control values. Microvascular perfusion was preserved in the LPS+20%HSA group at t3, whereas alterations reappeared in the LPS+4%HSA group. Conclusions In a rat model of normotensive endotoxemia, the infusion of 4% or 20% HSA produced a similar acute improvement in the microvascular perfusion in otherwise unresuscitated animals.
Interictal Paroxysmal Epileptic Discharges during Sleep in Childhood: Phenotypic Variability in a Family

Summary: We report three children of the same parents who exhibited various types of cognitive disorders, ranging from severe mental deficiency with transient autistic‐like regression to specific neuropsychological disabilities, associated with paroxysmal EEG abnormalities with various patterns of severity. This familial association highly suggests a genetic factor responsible for both epileptic discharges on EEG and cognitive dysfunction.
The evolutionary split of Pinaceae from other conifers: evidence from an intron loss and a multigene phylogeny.

The second intron in the mitochondrial gene nad1 was surveyed using PCR, DNA sequencing, or Southern hybridization in 323 species (313 genera, 212 families) of seed plants. The intron was absent in all 22 species (22 genera, 8 families) of non-Pinaceae conifers studied, in Welwitschia mirabilis, and in seven angiosperms. Whereas absence of the intron in seven angiosperms and Welwitschia is likely due to seven independent losses when evaluated against the recently published multigene phylogenies, the lack of the intron in all non-Pinaceae conifers can be best explained by a single loss. These data suggest that the non-Pinaceae conifers represent a monophyletic group. We also conducted a phylogenetic analysis of seed plants using a combined data set of the partial exon and intron sequences of nad1 generated from this study and published sequences of mitochondrial cox1 and small subunit (SSU) rDNA, chloroplast rbcL, and nuclear 18S rDNA. The results supported the split of conifers into two groups: Pinaceae and non-Pinaceae conifers. The Gnetales were sister to Pinaceae, in agreement with the conclusion from other recent molecular phylogenetic studies that refute the anthophyte hypothesis.
The electroencephalogram after alcohol administration in high-risk men and the development of alcohol use disorders 10 years later.

BACKGROUND In 1979 through 1980, electroencephalographic (EEG) responses to an alcohol challenge in 19 year-old sons of alcoholics as well as in sons of nonalcoholic control subjects were examined. The familial risk status of the subjects and greater EEG sensitivity to alcohol were hypothesized to predict the development of alcoholism 10 years later. METHODS In 1990 through 1992, diagnostic interviews were completed to ascertain alcohol and other substance use disorders in these subjects and to update their family history. RESULTS Updated family history of alcoholism predicted the development of substance dependence. Density of alcoholic relatives (the number of alcoholic relatives divided by the number of known relatives) was positively related to the severity of alcohol use disorders in the probands. Contrary to expectation, a greater EEG response at age 19 years was not related to the later development of alcohol dependence. Instead, the opposite was observed: a smaller EEG alpha frequency response to alcohol at age 19 years was related to the development of alcohol dependence and high quantity and frequency of alcohol consumption 10 years later. CONCLUSIONS Lower EEG response to a small dose of alcohol may be associated with the later development of alcohol dependence. This result is based on a small number of subjects and should be interpreted with caution. Although this result is opposite to our 1980 hypothesis, it is consistent with much of the recent literature.
The Electron Microscopy of Bacteriophage-like Particles in Dental Plaque

The morphology of bacteriophage-like particles contained in samples of dental plaque is described. The phage-like particles were observed within fusiform-shaped bacteria and in clumps between bacteria. The particles were hexagonal in cross section, approximately 1,100 nm in diameter and contained an electron-dense core. In areas of cell lysis, tail forms were observed both free and in association with the particles. Occasional particles were attached to bacterial cell walls by means of shortened tailpieces.
Phase-Controlled Quadruple ${LC}_{p}$  Resonant Inverter to Drive 600-W HPS Lamps

This paper presents the analysis and design method of a new configuration of resonant inverter suitable for high-intensity discharge (HID) lamp ballast applications. The proposed inverter results from the parallel connection of four class D LCp resonant inverters, sharing the total load current. In this way, the conduction losses are reduced and high frequency operation is achieved for high power lamps. The power control is implemented at constant frequency, modifying the phase displacement among the output voltages of each inverter section. Considering that the deviation of the operation point caused by the control action deteriorates the converter efficiency, this design guarantees minimum control action to correct the lamp power deviation caused by the lamp aging. A 600-W prototype has been built to validate the design proposal
Reaching and identifying the STD/HIV risk of sex workers in Beijing.

China's HIV cases are growing by more than 30% annually. Few researchers have been able to reach sex workers and examine their risk behavior patterns. Key informants in illegal prostitution connected with owners of establishments in Beijing to recruit the involvement of sex workers. A total of 69 were surveyed about their background and risk behaviors in spring, 2000 in four venues; hair salons, bathing centers, karaoke bars, and the street. The women were mostly young (in their 20s), of low socioeconomic status, and from small towns and villages. They worked about 3 to 4 days a week, averaged one to two clients per day, made the equivalent of about U.S. $135 a week, and averaged about 3 months at any one establishment. They lacked accurate knowledge of STDs/HIV, and although 61% reported consistent condom usage 93% associated usage with prevention of pregnancy and 72% perceived a low risk of HIV infection. Nearly all (98%) felt condoms were affordable, yet 37% reported they were not accessible; 74% had obtained a medical exam in the last year, but 29% were examined when sick; only 10% received prevention information during medical visits, and 55% did not know where to go for HIV testing. Based on venue, stratification among sex workers existed, impacting their risk. Sex workers are at high risk of HIV infection/transmission, especially as the virus becomes more established in Beijing. Although risk factors were consistent with those of sex workers in other countries, the variation by venue, the low perceived vulnerability to HIV, the highly illegal nature of prostitution, and high mobility of sex workers in Beijing calls for a tailored intervention approach. Prevention messages/strategies should be responsive to the differing background, knowledge,
Pivotal Role of Reactive Oxygen Species as Intracellular Mediators of Hyperthermia-induced Apoptosis*

The effects of cellular antioxidant capacity on hyperthermia (HT)-induced apoptosis and production of antiapoptotic heat shock proteins (HSPs) were investigated in HL-60 cells and in HL-60AR cells that are characterized by an elevated endogenous catalase activity. Exposure of both cell lines to 43 °C for 1 h initiated apoptosis. Apoptosis peaked at 3–6 h after heat exposure in the HL-60 cells. Whereas HL-60AR cells were partially protected against HT-induced apoptosis at these early time points, maximal levels of apoptosis were detected later, i.e. 12–18 h after heat exposure. This differential induction of apoptosis was directly correlated to the induction of the antiapoptotic HSP27 and HSP70. In particular, in the HL-60 cells HSP27 was significantly induced at 12–18 h after exposure to 43 °C when apoptosis dropped. In contrast, coinciding with the late onset of apoptosis in HL-60AR cells at that time HL-60AR cells lacked a similar HSP response. In line with the higher antioxidant capacity HL-60AR cells accumulated reactive oxygen species to a lesser degree than HL-60 cells after heat treatment. Protection from HT-induced apoptosis as well as diminished heat-induced HSP27 expression was also observed after cotreatment of HL-60 cells with 43 °C and catalase but not with superoxide dismutase. These data emphasize the pivotal role of reactive oxygen species for HT induced pro- and antiapoptotic pathways.
Femtosecond excitonic relaxation dynamics of perovskite on mesoporous films of Al₂O₃ and NiO nanoparticles.

The excitonic relaxation dynamics of perovskite adsorbed on mesoporous thin films of Al2O3 and NiO upon excitation at 450 nm were investigated with femtosecond optical gating of photoluminescence (PL) via up-conversion. The temporal profiles of emission observed in spectral region 670-810 nm were described satisfactorily with a composite consecutive kinetic model and three transient components representing one hot and two cold excitonic relaxations. All observed relaxation dynamics depend on the emission wavelength, showing a systematic time-amplitude correlation for all three components. When the NiO film was employed, we observed an extent of relaxation proceeding through the non-emissive surface state larger than through the direct electronic relaxation channel, which quenches the PL intensity more effectively than on the Al2O3 film. We conclude that perovskite is an effective hole carrier in a p-type electrode for NiO-based perovskite solar cells showing great performance.
Platelet-derived Growth Factor-specific Regulation of theJE Promoter in Rat Aortic Smooth Muscle Cells*

JE is a member of the family of “immediate early” genes induced by growth factors and cytokines.JE encodes a low molecular weight secretory glycoprotein analogous to the human monocyte chemoattractant protein, MCP-1. JE and MCP-1 proteins are thought to play an important role in inflammation and in the recruitment of monocyte/macrophages to the vessel wall during the development of atherosclerosis. We have previously reported that the induction of JE in rat aortic smooth muscle cells (SMC) was specific to platelet-derived growth factor (PDGF) and was not seen with other growth agonists. Using a luciferase reporter system and transient transfection assays of rat aortic SMC, we now report the identification of a region in the proximal rat JE promoter that is responsive to PDGF but not to other growth factors (angiotensin II and α-thrombin) or cytokines (interleukin 1-β and tumor necrosis factor-α). The full response to PDGF (∼6-fold) requires the cooperative activity of two potentially novel cis-acting elements, at positions −146 to −128 and −84 to −59. While each element produces a different pattern in electrophoretic mobility shift assays, they appear to bind the same PDGF-responsive species. Further analysis of these regions should provide important insights into PDGF-specific responses in vascular SMC.
AN INVESTIGATION OF APPROACHES TO COMPUTERIZING THE GRE® SUBJECT TESTS

In this project, we explored the application of two common, computer-based test (CBT) designs, computerized adaptive testing (CAT) and linear-on-the-fly testing (LOFT) to two Graduate Record Examination (GRE) Subject Tests chosen to represent the range of different test structures: the GRE Mathematics Test and the GRE Biology Test. The results were mixed. Some variations proved adequate in meeting minimum psychometric requirements. However, no variation proved adequate in the implementation of a complete test blueprint, nor did any variation provide strong support for meeting test security requirements in an environment of unrestricted continuous testing. The conversion of the GRE Subject Tests from paper-and-pencil administration to a computer-based testing format seems viable only if pretesting is implemented, item writing is expanded, test length is reduced, classification schemes are improved, and the roles of test specialists and the Committees of Examiners are substantially revised.
ANTIMICROBIAL THERAPY OF RATBITE FEVER. A REVIEW.

RATBITE fever is an acute illness caused by Streptobacillus moniliformis or Spirillum minus , and characterized by chills, rash, and intermittent or relapsing fever. Arthritis or a local lesion at the bite-site, usually associated with regional lymphadenopathy, may also occur with the onset of other symptoms, depending upon the causative organism involved. Although penicillin is accepted as the treatment of choice for the disease, no agreement can be found in the literature concerning the most desirable dosage and duration of therapy. This paper reports a case of streptobacillary-ratbite fever successfully treated with penicillin. Previous American, Canadian, and British experience with cases in which the causative microorganisms were clearly demonstrated are reviewed, and the therapeutic programs used have been compared with the results achieved in an attempt to determine optimal management with antimicrobials now available. Report of a Case A 38-year-old white male radioisotope laboratory technician was admitted to the medical service
FIVE‐YEAR FOLLOW‐UP OF FUNCTIONAL SITTING POSITION IN CHILDREN WITH CEREBRAL PALSY

Ten children with cerebral palsy (CP) were videotaped and photographed after the introduction to a functional sitting position (FSP–defined as a position in which children with CP could gain head‐, trunk‐ and foot‐control and the maximum degree of independent function when performing arm and hand movements during tasks) and again five years later. Head‐, trunk‐ and foot‐control, and arm‐and hand‐function were assessed from video‐tapes by the Sitting Assessment Scale. The eight children who had been using the l‐'SP throughout this period showed slight but significant improvement; the remaining two had deteriorated. Since all 10 children had undergone similar rehabilitation programmes, it is concluded that sitting in an FSP contributed to their improvement in the ability to use the hand and arm.
Actions of the novel neuroprotective agent, lifarizine (RS‐87476), on voltage‐dependent sodium currents in the neuroblastoma cell line, N1E‐115

1 The actions of the neuroprotective agent, lifarizine (RS‐87476‐190), on voltage‐dependent Na+ currents have been examined in the neuroblastoma cell line, N1E‐115, using the whole‐cell variant of the patch clamp technique. 2 At a holding potential of −80 mV, lifarizine reduced the peak Na+ current evoked by a 10 ms depolarizing step with an IC50 of 1. 3μm. At holding potentials of −100 and −60mV the IC50 concentrations of lifarizine were 7.3 μm and 0. 3μm, respectively. 3 At a holding potential of −100 mV, most channels were in the resting state and the IC50 value for inhibition of Na+ current should correspond to the dissociation constant of lifarizine for resting channels (KR). Kr was therefore estimated to be 7.3 μm. 4 In the absence of lifarizine, recovery from inactivation following a 20 s depolarization from −100 mV to 0 mV was complete within 2 s. However, in the presence of 3 μm lifarizine recovery took place in a biexponential fashion with time constants of 7 s and 79 s. 5 Lifarizine (1 μm) had no effect on steady‐state inactivation curves when conditioning pre‐pulses of 1 s duration were used. However, when pre‐pulse durations of 1 min were used the curves were shifted to the left by lifarizine by about 10 mV. Analysis of the shifts induced by a range of lifarizine concentrations revealed that the apparent affinity of lifarizine for the inactivated state of the channel (KI) was 0.19 μm. 6 Lifarizine (1 μm) had no effect on chloramine‐T‐modified Na+ currents, suggesting no significant open channel interaction. 7 Taken together, these data show that lifarizine is a potent voltage‐dependent inhibitor of Na+ currents in N1E‐115 cells and that the voltage‐dependence arises from an interaction of the compound with the inactivated state of the channel. The possible contribution of Na+ current inhibition to the neuroprotective actions of lifarizine is discussed.
Prediction of heart disease at early stage using data mining and big data analytics: A survey

In this paper, the various technologies of data mining (DM) models for forecast of heart disease are discussed. Data mining plays an important role in building an intelligent model for medical systems to detect heart disease (HD) using data sets of the patients, which involves risk factor associated with heart disease. Medical practitioners can help the patients by predicting the heart disease before occurring. The large data available from medical diagnosis is analyzed by using data mining tools and useful information known as knowledge is extracted. Mining is a method of exploring massive sets of data to take out patterns which are hidden and previously unknown relationships and knowledge detection to help the better understanding of medical data to prevent heart disease. There are many DM techniques available namely Classification techniques involving Naïve Bayes (NB), Decision tree (DT), Neural network (NN), Genetic algorithm (GA), Artificial intelligence (AI) and Clustering algorithms like K-NN, and Support vector machine (SVM). Several studies have been carried out for developing prediction model using individual technique and also by combining two or more techniques. This paper provides a quick and easy review and understanding of available prediction models using data mining from 2004 to 2016. The comparison shows the accuracy level of each model given by different researchers.