Miracle-dz/UniFAR-FTUs huggingface dataset:
Supraclavicular Ultrasound-Guided Catheterization of the Subclavian Vein in Pediatric and Neonatal ICUs: A Feasibility Study

Objectives: To assess the feasibility of ultrasound-guided supraclavicular catheterization of the subclavian vein in pediatric and neonatal ICU. Design: Retrospective cohort. Setting: Ten-bed pediatric medicosurgical ICU and 15-bed neonatal ICU. Patients: Children and newborns undergoing supraclavicular ultrasound-guided subclavian vein catheterization from March 2010 to September 2010. Measurements: The placement of central venous catheter in ICU was carried out either by one of the experts in ultrasound-guided puncture of the unit or by a novice completely supervised by the expert. The success and the early complications were recorded. A comparison between novice and expert operators was also made. Results: Forty-two catheters were placed in 40 children. The median age and median weight were, respectively, 6.5 months and 6.5 kg. The success rate was 97.6% (one failure) and the early complication rate was 4.7% (one pneumothorax and one arterial puncture); 61% of children breathed spontaneously during the catheter placement. No significant difference was found between expert and novice operators. Conclusions: Supraclavicular ultrasound-guided catheterization of the subclavian vein in pediatric and neonatal ICU seems to be a promising technique in the context of emergency. It is safe, reliable, with few early complications. Furthermore, it does not compromise the airways of the patient owing to the low level of sedation needed for its placement.
Cross-mating experiments with Boophilus annulatus and B. microplus (Acarina: Ixodidae).

Female Boophilus annulatus (Say) and B. microplus (Canestrini) mated readily with males of the other species when pairs were isolated in a small cell on a calf's back. The crossmated females engorged as readily as females that mated with their own species, the size and fecundity of the females were not altered by cross-mating, the hatch of the F1 eggs equaled that of colony strains of the 2 species, and the F1 hybrid progeny appeared normal in vigor and longevity. However, the eggs resulting from F1 brother × sister matings generally did not hatch. Backcrosses indicated that the sterility of hybrid males exceeded 99% and that the fertility of hybrid females was reduced. The caudal process of the hybrid males was usually intermediate in size between that of normal B. annulatus and B. microplus males. The 2 species are apparently closely related, probably sibling species, but reproductively isolated by sterility of hybrids.
Speech rate in children with and without phonological disorder.

BACKGROUND Speech rate in phonological disorders. AIM To compare the performance of children with and without phonological disorders in different speech rate tests. METHOD Participants of this study were 20 children diagnosed with phonological disorders (PDG) and 20 with typical speech development (CG), aged 4 years to 10 years and 11 months, of both genders. Speech rate measurements (total duration time, syllables per second and phonemes per second) were analyzed in two imitation tests, being one standard and the other based on phrases taken from the child's own speech, each composed of a short and a long sentence. RESULTS The CG presented a significantly better performance than the PDG, when considering all of the measurements applied to the standard imitation test and also when considering the total duration time of the long sentence in the self-imitation test. This indicates that the length and type of sentences influenced the performance of both groups. CONCLUSION Lower values of speech rate are observed for children with PD, participants of this study, due to possible language or motor deficits, although there is evidence of speech rate control due to the length of the sentence. All of the measurements indicated this difference, specially for the standard imitation test.
Possible death of a buffalo calf ( Syncercus caffer ) due to suspected heartwater ( Ehrlichia ruminantium )

INTRODUCTION Cowdria ruminantium, the causative organism of heartwater, has recently been reclassified as Ehrlichia ruminantium based on genetic analysis as well as biological and protein characteristics. Ehrlichia ruminantium is transmitted by 3 host ticks belonging to the genus Amblyomma. Amblyomma hebraeum, the vector of E. ruminantium in South Africa, occurs mainly in the Limpopo, KwaZulu-Natal and in the Eastern Cape provinces of South Africa. Heartwater is one of the most important tick-borne diseases of domestic ruminants in South Africa. Mortality rates are high in susceptible cattle, sheep and goats introduced into endemic regions and peracute, acute, subacute and clinically inapparent forms of heartwater have been reported in these domestic ruminants. In wild ruminants, such as the blue wildebeest (Connochaetes taurinus), springbok (Antidorcas marsupialis), bushbuck (Tragelaphus scriptus), black wildebeest (Connochaetes gnu), blesbok (Damaliscus albifrons), steenbok (Raphicerus campestris), sitatunga (Tragelaphus spekei) and eland (Taurotragus oryx) deaths due to infection with E. ruminantium have been reported. To date, no deaths due to heartwater have been documented in the African buffalo. However, African buffalo as well as eland, kudu (Tragephalus strepsiceros strepsiceros), blue wildebeest, giraffe (Giraffa camelopardalis), sable (Hippotragus equinus) and tsessebe (Damaliscus lunatus) are known to seroconvert after artificial infection and become carriers of the parasite. One African buffalo showed signs of heartwater after artificial infection with E. ruminatium, but it recovered spontaneously. Different strains of E. ruminant ium have the abil i ty to grow in endothelial cell lines of sable antelope, buffalo and eland. These findings suggest the possibility that wild ruminants play an important role in the epidemiology and spread of heartwater. This hypothesis is strengthened by the results of a study in the Kruger National Park of South Africa where it was shown that a wildlife cycle can maintain the transmission of E. ruminantium independent of domestic ruminants. Numerous E. ruminantium isolates have been made, and named after the farm or country of origin. These strains differ in their pathogenicity for ruminants.
[Effect of continuous positive airway pressure on erectile dysfunction in patients with obstructive sleep apnea syndrome: a meta-analysis].

OBJECTIVE To evaluate the effect of continuous positive airway pressure (CPAP) on erectile dysfunction (ED) in patients with obstructive sleep apnea syndrome (OSAS). METHODS We searched Cochrane Library, PubMed, China Academic Journal Full-Text Database, Chinese Biomedical Literature Database, Wanfang Resource Database and Chinese Journal Full-Text Database for clinical trials on the effect of CPAP on ED in OSAS patients. We identified the trials according to inclusion and exclusion criteria, evaluated their quality, and then extracted valid data for meta-analysis. RESULTS We included 4 articles, 3 in English and 1 in Chinese, involving 77 cases of OSAS with ED. Meta-analysis revealed no statistically significant heterogeneity among different studies (P = 0.80; I2 = 0%), and therefore the fixed effect model was used for the analysis, which showed a significant increase in the IIEF-5 score after CPAP treatment (WMD = 4.19, 95% [3.01, 5.36], P < 0.001). CONCLUSION The existing evidence from clinical trials shows that the CPAP therapy can significantly improve ED in OSAS patients. However, its efficacy has to be verified by randomized controlled clinical trials of higher quality and larger sample size.
Visualization of satellite cells in living muscle fibres of the frog

Satellite cells were visualized in living muscle fibres of the frog. Single fibres or bundles consisting of a few fibres were isolated after treatment with collagenase, and viewed under the light microscope. Subsequent electron microscopy of identified cells confirmed that they were satellite muscle cells. Under the light microscope, satellite cells appear as fusiform cells, tapering into long fine processes usually orientated parallel to the muscle fibre axis. Horseradish peroxidase injected into the muscle fibre was not transferred to the satellite cells.
Design and characteristics measurement of a high-speed CMOS imaging system

This paper introduces the design of a high-speed 2K × 2K CMOS imaging system that is part of a Wide Field Monitor System proposed in China recent years. The CMOS sensor used in this imaging system is LUPA-4000 from Cypress corp. This CMOS camera is composed of an analogue system and a digital embedded system. The digital embedded system integrated with an NIOS II soft-core processor serves as the control and data acquisition system of the camera. In addition, research on characteristics measurement for CMOS imagers was carried out, including readout noise, linearity, quantum efficiency, pixel non-uniformity, dark current, full-well capacity, and gain. The photon transfer technique is adopted to evaluate the gain and readout noise. At last, the evaluation results of the high-speed CMOS imaging system are shown. The results indicate that this high-speed CMOS camera can meet the requirements of the whole Wide Field Monitor System.
Initiation of V(D)J recombination in vivo: role of recombination signal sequences in formation of single and paired double‐strand breaks

In V(D)J recombination, double‐strand breaks (DSBs) are introduced at recombination signal sequences (RSSs) which consist of three distinct elements: a heptamer, a 12 or 23 nucleotide spacer and a nonamer. Efficient DSB formation requires a 12/23 RSS pair and occurs at both RSS in a temporally coupled fashion (coupled cleavage). It remains unknown which RSS elements are important for coupled cleavage. Furthermore, it has not been established whether some RSS components are critical only for cleavage in cis, with others mainly promoting cleavage in trans at the partner RSS. We investigated these questions by analyzing the effects of RSS mutations on the formation of DSBs in vivo. The abundance of DSBs in cis (at the mutant RSS) and in trans (at the consensus RSS) was determined using an established ligation‐mediated PCR assay. We also developed a Southern blotting approach that allows the first direct measurement of dual and single RSS cleavage in vivo. Our results demonstrate that the heptamer, spacer and nonamer elements are all required for coupled cleavage in vivo. These studies also provide evidence for cleavage events involving a single RSS both in mutant substrates and in substrates containing a consensus 12/23 RSS pair.
FAT10 Plays a Role in the Regulation of Chromosomal Stability*

Aneuploidy is a key process in tumorigenesis. Dysfunction of the mitotic spindle checkpoint proteins has been implicated as a cause of aneuploidy in cells. We have previously reported that FAT10, a member of the ubiquitin-like modifier family of proteins, is overexpressed in several gastrointestinal and gynecological cancers. Here we show that FAT10 interacts with MAD2, a spindle checkpoint protein, during mitosis. Notably, we show that localization of MAD2 at the kinetochore during the prometaphase stage of the cell cycle was greatly reduced in FAT10-overexpressing cells. Furthermore, compared with parental HCT116 cells, fewer mitotic cells were observed after double thymidine-synchronized FAT10-overexpressing cells were released into nocodazole for more than 4 h. Nonetheless, when these double thymidine-treated cells were released into media, a similar number of G1 parental and FAT10-overexpressing HCT116 cells was observed throughout the 10-h time course. Additionally, more nocodazole-treated FAT10-overexpressing cells escape mitotic controls and are multinucleate compared with parental cells. Significantly, we observed a higher degree of variability in chromosome number in cells overexpressing FAT10. Hence, our data suggest that high levels of FAT10 protein in cells lead to increased mitotic nondisjunction and chromosome instability, and this effect is mediated by an abbreviated mitotic phase and the reduction in the kinetochore localization of MAD2 during the prometaphase stage of the cell cycle.
Bacterial cytokinesis: From Z ring to divisome

Ancestral homologues of the major eukaryotic cytoskeletal families, tubulin and actin, play critical roles in cytokinesis of bacterial cells. FtsZ is the ancestral homologue of tubulin and assembles into the Z ring that determines the division plane. FtsA, a member of the actin family, is involved in coordinating cell wall synthesis during cytokinesis. FtsA assists in the formation of the Z ring and also has a critical role in recruiting downstream division proteins to the Z ring to generate the divisome that divides the cell. Spatial regulation of cytokinesis occurs at the stage of Z ring assembly and regulation of cell size occurs at this stage or during Z ring maturation. © 2012 Wiley Periodicals, Inc
Adsorption of cubic liquid crystalline nanoparticles on model membranes

The interactions of lipid based cubic liquid crystalline nanoparticles (Cubosome®) with surface supported model membranes constituted of dioleylphosphatidylcholine (DOPC) have been studied in situ by use of ellipsometry, quartz crystal microbalance with dissipation monitoring and neutron reflectivity. The systems investigated were cubic phase dispersions of glycerol monooleate (GMO) stabilised by a non-ionic block copolymer, Pluronic®F-127. The interaction between the cubic nanoparticles and the lipid bilayer is a dynamic process where the nanoparticles initially adsorb at the bilayer surface. Interfacial lipid exchange takes place where GMO is delivered into the bilayer and DOPC is extracted into the nanoparticle (34% loss). A subsequent release of the adsorbates can be triggered when the solution concentration exceeds 0.002 mg ml−1. The release shows that the attractive interaction between the cubic nanoparticles and lipid bilayer is unstable after sufficient exchange of material takes place. This instability is indicative of a local phase separation at the interface between the bilayer and the nanoparticles, which causes desorption of nanoparticles. Some particles remain attached to the bilayer even hours after the initial interaction. The ability to trigger the release of the nanoparticles through increasing the solution concentration offers exciting potential in the design of drug delivery aids.
Biocurcumin as Radiosensitiser for Cervical Cancer Study (BRACES): A Double-Blind Randomised Placebo-Controlled Trial

Cervical cancer is a leading cause of death among women worldwide, particularly in Indonesia. The main treatment of advanced-stage cervical cancer is radiation; however, the outcomes do not meet the required expectations. [1,7-Bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5dione] has been reported in several studies for its potency in cancer therapy. This study aims to investigate the clinical and molecular [(malondialdehyde (MDA) and NF-κB levels] effects, apoptotic index, and safety of Biocurcumin (BCM-95) as a radiosensitiser in cervical cancer. In this double-blind placebo randomised-controlled trial, we randomised 121 patients into 2 groups (BCM-95 or placebo). MDA and their NF-κB levels and apoptotic index were measured before and after administering 24 Gy of radiation. MDA was identified using Wills' method, whereas NF-κB was identified via ELISA. The apoptotic index was identified using TUNEL and DAPI staining. The clinical response was classified based on the RECIST. MDA levels before radiation were similar between both groups in per protocol and intention-to-treat (ITT) analyses (p = 0.53 and p = 0.16, respectively). After radiation, MDA levels increased in both groups with no significant differences in per protocol and ITT analyses (p = 0.52 and p = 0.18, respectively). NF-κB levels before radiation were similar between the two groups in per protocol and ITT analyses (p = 0.92 and p = 0.98, respectively). After radiation, the BCM-95 group showed an increase in the NF-κB levels compared with the placebo group in per protocol analysis but not in ITT analysis (p = 0.018 and p = 0.42, respectively). The BCM-95 group had a higher apoptotic index before radiation in per protocol analysis but not in ITT analysis (p = 0.01 and p = 0.61, respectively). After radiation, the apoptotic index remained higher in the BCM-95 group in per protocol analysis but not in ITT analysis (p = 0.04 and p = 0.91, respectively). There was no significant difference in complete response between the groups (per protocol, p = 0.61; ITT analysis, p = 0.90). Although BCM-95 can regulate ROS, NF-κB, and apoptosis in human cervical cancer, it is not significant. Therefore, BCM-95 does not improve clinical response to radiation treatment.
Targeting the ‘Janus face' of the B2-bradykinin receptor

Introduction: Kinins are main active mediators of the kallikrein–kinin system (KKS) via bradykinin type 1 inducible (B1R) and type 2 constitutive (B2R) receptors. B2R mediates most physiological bradykinin (BK) responses, including vasodilation, natriuresis, NO, prostaglandins release. Areas covered: The article summarizes knowledge on kinins, B2R signaling and biological functions; highlights crosstalks between B2R and renin–angiotensin system (RAS). The double role (Janus face) in physiopathology, namely the beneficial protection of the endothelium, which forms the basis for the therapeutical utilization of B2 receptor agonists, on the one side, and the involvement of B2R in inflammation or infection diseases and in pain mechanisms, which justifies the use of B2R antagonists, on the other side, is extensively analyzed. Expert opinion: For decades, the B2R has been unconsciously activated during angiotensin-converting enzyme inhibitor (ACEI) or angiotensin receptor blocker (ARB) treatments. Whether direct B2R targeting with stable agonists could bring additional therapeutic benefit to RAS inhibition should be investigated. Efficacy, established in experimental models, should be confirmed by translational studies in cardiovascular pathologies, glaucoma, Duchenne cardiopathy and during brain cancer therapy. The other face of B2R is targeted by antagonists already approved to treat hereditary angioedema. The use of antagonists could be extended to other angioedema and efficacy tested against acute pain and inflammatory diseases.
WUJUD KESANTUNAN BERBAHASA GURU: STUDI KASUS DI SD IMMERSION PONOROGO

Guru, digugu lan ditiru ungkapan tersebut menjadi salah satu latarbelakang dalam penelitian ini, sikap dan prilaku guru dapat menjadi contoh untuk siswanya termasuk dalam penggunaan bahasa. Seorang guru hendaknya menerapkan prinsip kesantunan berbahasa karena merupakan salah satu ciri khas seorang pendidik. Selain itu juga kesuksesan dalam berkomunikasi juga bergantung pola kesantunan dalam penggunaan bahasa. Tujuan dari penelitian ini (1) mendekripsikan bentuk kepatuhan dan pelanggaran maksim kesantunan berbahasa (2) mendeskripsikan alasan dan tujuan dari tuturan yang mengandung bentuk kepatuhan dan pelanggaran maksim kesantunan berbahasa. Penelitian ini menggunakan metode deskriptif dengan pendekatan kualitatif. Partisipan yang terlibat dalam penelitian ini adalah lima guru SD Immersion, Ponorogo. Data dikumpulkan melalui metode simak, yang terdiri dari tiga teknik, yaitu teknik simak, teknik rekam, dan teknik catat. Data dianalisis dengan langkah berikut: (1) mentranskripsikan data hasil rekaman ke dalam bentuk tulisan, (2) mengklasifikasikan bentuk kesantunan berbahasa menurut Leech, (3) menganalisis bentuk bentuk kesantunan berbahasa, (4) menganalisis faktor terjadinya kepatuhan dan pelanggaran kesantunan berbahasa, dan (5) menyimpulkan hasil analisis. Dari hasil penelitian yang telah dilakukan terdapat bentuk kepatuhan dan pelanggaran maksim kesantunan berbahasa oleh guru. Setiap pelanggaran kesantunan berbahasa oleh guru tidak memunyai tujuan yang kurang baik akan tetapi sebagai bentuk peringatan dan bentuk motivasi terhadap siswa.
A New Electromagnetic Launcher by Sextupole Rails: Electromagnetic Propulsion and Shielding Numerical Validation

A novel sextupole rails electromagnetic launcher has been designed and validated with numerical simulations of electromagnetic propulsion and shielding. The sextupole rails and club-shaped projectile are proposed as that the club-shaped projectile carrying the sextupole toroidal currents is surrounded by the sextupole toroidal field created from the sextupole rails. The interaction between the toroidal magnetic field and the orthogonal toroidal currents results in the generation of axial acceleration force. The sextupole rails electromagnetic launcher can solve the problems of magnetic shielding without shielding coils or materials. The theoretical assessment of the propulsion force and magnetic shielding is performed by corresponding numerical validation based on finite-element simulations. The numerical simulation results demonstrate that the sextupole rails electromagnetic launcher represents high thrust/current ratios and productive magnetic shielding effect.
Large deformation of corrugated sandwich panels under three-point bending

Corrugated sandwich panels are widely used in engineering applications for their excellent energy absorption and lightweight. In this research, the mechanical response of aluminum corrugated sandwich panels subjected to three-point bending is investigated experimentally, numerically, and theoretically. In the experiments, the sandwich panels were loaded under two conditions, namely base indentation and node indentation. A parametric study is conducted by ABAQUS/explicit to investigate the effects of geometric configurations (corrugation angle, core height, and core thickness) on the deformation mode, peak force, and energy absorption. Both peak force and specific energy absorption vary with the geometric parameters. Theoretical models are further developed to predict the force–displacement curves of the panels under the two loading conditions. The theoretically predicted crushing force is in good agreement with both the experimental and simulated results. Finally, the non-dominated sorting genetic algorithm II is adopted to optimize the geometric configuration to improve the specific energy absorption and reduce the weight of corrugated sandwich panels.
Thought outside the box: Intensive care unit freakonomics and decision making in the intensive care unit

Despite concerted efforts to improve the quality of care provided in the intensive care unit, inconsistency continues to characterize physician decision making. The resulting variations in care compromise outcomes and impose unnecessary decisional regret on clinicians and patients alike. Critical care is not the only arena where decisions fail to conform to the dictates of logic. Behavioral psychology uses scientific methods to analyze the influence of social, cognitive, and emotional factors on decisions. The overarching hypothesis underlying this “thought outside the box” is that the application of behavioral psychology to physician decision making in the intensive care unit will demonstrate the existence of cognitive biases associated with classic intensive care unit decisions; provide insight into novel strategies to train intensive care unit clinicians to better use data; and improve the quality of decision making in the intensive care unit as characterized by more consistent, patient-centered decisions with reduced decisional regret and work-related stress experienced by physicians.