Miracle-dz/UniFAR-FTUs huggingface dataset:
Vancomycin production is enhanced in chemostat culture with biomass-recycle.

Production of the glycopeptide antibiotic vancomycin by Amycolatopsis orientalis ATCC 19795 was examined in phosphate-limited chemostat cultures with biomass-recycle, employing an oscillating membrane separator, at a constant dilution rate (D= 0. 14 h-1). Experiments made under low agitation conditions (600 rpm) showed that the biomass concentration could be increased 3.9-fold with vancomycin production kinetics very similar to that of chemostat culture without biomass-recycle. The specific production rate (qvancomycin) was maximal when the biomass-recycle ratio (R) was 0.13 (D= 0.087 h-1). When the dissolved oxygen tension dropped below 20% (air saturation), the biomass and vancomycin concentrations decreased and an unidentified red metabolite was released into the culture medium. Using increased agitation (850 rpm), used to maintain the dissolved oxygen tension above 20% air saturation, maximum increases in biomass concentration (7.9-fold) and vancomcyin production 1.6-fold (0.6 mg/g dry weight/h) were obtained when R was 0.44 (D= 0.056 h -1) compared to chemostat culture without biomass-recycle. Moreover, at this latter recycle ratio the volumetric vancomycin production rate was 14.7 mg/L/h (a 7-fold increase compared to chemostat culture without biomass-recycle). These observations encourage further research on biomass-recycling as a means of optimising the production of antibiotics.
Real Exchange Returns and Real Stock Price Returns in Nigeria: An Econometrics Analysis of the Direction of Causality

This paper examined the causal relationship between real exchange rate returns and real stock price returns in Nigeria from January 1985- June 2017. For the investigation the VAR/pair-wise granger causality test and Sims-causality test were applied. From the evidences shown, there exist a unidirectional causal relationship between real exchange rate returns and real stock price returns. Causality running from Real exchange rate returns to real stock price returns. Thus, the past values of REXR can influence/predict the present value of RSPR. This confirms the findings of Olugbenga (2012) and the proposition of the flow oriented model. Also, evidences from the sims-Causality test show that there is uni-directional causality running from Real exchange rate returns to real stock price returns. Thus, the present value of REXR can influence/predict the future values of RSPR. Therefore, it is important for the monetary authority of Nigeria to put into due consideration the exchange rate policy in its conduct of monetary policy internally. Investors could also use these findings as an effective tool in stock trading. As movement in the foreign exchange market (real exchange rate returns) could have a great impact on the present and future movement of stock exchange market (real stock price returns) in Nigeria.
Three telomerases with completely non‐telomeric template replacements are catalytically active

Telomerase is a reverse transcriptase minimally composed of a reverse transcriptase protein subunit and an internal RNA component that contains the templating region. Point mutations of template RNA bases can cause loss of enzymatic activity, reduced processivity and misincorporation in vitro. Here we report the first complete replacement of the nine base Tetrahymena thermophila telomerase templating region in vivo with non‐telomeric sequences. Rather than ablating telomerase activity, three such replaced telomerases (U9, AUN and AU4) were effective in polymerization in vitro. In vivo, the AU4 and AUN genes caused telomere shortening. We demonstrated the fidelity of the AUN and U9 telomerases in vitro and utilized AUN telomerase to demonstrate that 5′ end primer recognition by telomerase is independent of template base pairing. However, the mutant AUN template telomerase catalyzed an abnormal DNA cleavage reaction. For these U‐only and AU‐ substituted templates, we conclude that base‐specific interactions between the telomerase template and protein (or distant parts of the RNA) are not absolutely required for the minimal core telomerase functions of nucleotide addition and base discrimination.
Improving Basic Football Technique Skill through Small Sided Games

: The purpose of this study is to see the impact of the implementation of the form of Small Sided Games training on improving the skill of basic techniques of playing football. The basic techniques that become the focus of this research are: passing, dribbling and shooting. The research method used is experimental method and research design of one group pretest-posttest group design on football athlete’s PS UPI U-19 who were decided by purposive sampling. This stage begins with an initial test of basic engineering skills, then is treated by using the Small Sided Games and ends with a final test. Data obtained from the pre and post-test and processed by using SPSS. The result shows that there is a significant improvement in the basic soccer skills by using the form of the Small Sided Games exercise.
Interleukin‐1β and lnterleukin‐6 Stimulate Neurohypophysial Hormone Release in vitro

Interleukin‐1 (IL‐1) and interleukin‐6 (IL‐6) have been reported to stimulate the release of corticotrophin‐releasing hormone (CRN) in vitro, the response being antagonized by the cyclo‐oxygenase inhibitor, indomethacin. The effects of cytokines on the other major ACTH‐releasing hormone, vasopressin (AVP), and the other neurohypophysial hormone, oxytocin, have been little studied, and the published data are conflicting. We have therefore used a previously validated rat hypothalamic expiant model to evaluate whether IL‐1β and IL‐6 can directly activate the AVP and oxytocin neurosecretory system. In addition, we have also investigated the effects of inhibition of cyclo‐oxygenase (CO) and lipoxygenase (LO) activities on the stimulated release of AVP and oxytocin by means of a series of antagonists, including a specific LO pathway inhibitor. The static rat hypothalamic incubation system used involves fresh hypothalamic expiants with consecutive 20‐min incubations, and estimation of AVP and oxytocin concentrations in the medium by specific and sensitive radioimmuno‐assays. It was found that IL‐1β produced a dose‐dependent increase in the release of AVP and oxytocin at doses of 10 and 100 U/ml (P<0.005). Only at the higher dose of 100 U/ml was IL‐6 able to increase significantly AVP and oxytocin release (P<0.05). These stimulatory effects of IL‐1β and IL‐6 were blocked by cyclo‐oxygenase inhibitors, indomethacin (28 μM) and ibuprofen (100 nM), but not by the lipooxygenase inhibitor, BW A4C (10 μg/ml), suggesting that prostaglandins are involved in this process. Thus, cytokines are clearly able to modulate the neurohypophysial system in vitro, the effects probably being mediated by cyclo‐oxygenase products.
Incidence and types of adverse events and negligent care in Utah and Colorado.

BACKGROUND The ongoing debate on the incidence and types of iatrogenic injuries in American hospitals has been informed primarily by the Harvard Medical Practice Study, which analyzed hospitalizations in New York in 1984. The generalizability of these findings is unknown and has been questioned by other studies. OBJECTIVE We used methods similar to the Harvard Medical Practice Study to estimate the incidence and types of adverse events and negligent adverse events in Utah and Colorado in 1992. DESIGN AND SUBJECTS We selected a representative sample of hospitals from Utah and Colorado and then randomly sampled 15,000 nonpsychiatric 1992 discharges. Each record was screened by a trained nurse-reviewer for 1 of 18 criteria associated with adverse events. If > or =1 criteria were present, the record was reviewed by a trained physician to determine whether an adverse event or negligent adverse event occurred and to classify the type of adverse event. MEASURES The measures were adverse events and negligent adverse events. RESULTS Adverse events occurred in 2.9+/-0.2% (mean+/-SD) of hospitalizations in each state. In Utah, 32.6+/-4% of adverse events were due to negligence; in Colorado, 27.4+/-2.4%. Death occurred in 6.6+/-1.2% of adverse events and 8.8+/-2.5% of negligent adverse events. Operative adverse events comprised 44.9% of all adverse events; 16.9% were negligent, and 16.6% resulted in permanent disability. Adverse drug events were the leading cause of nonoperative adverse events (19.3% of all adverse events; 35.1% were negligent, and 9.7% caused permanent disability). Most adverse events were attributed to surgeons (46.1%, 22.3% negligent) and internists (23.2%, 44.9% negligent). CONCLUSIONS The incidence and types of adverse events in Utah and Colorado in 1992 were similar to those in New York State in 1984. Iatrogenic injury continues to be a significant public health problem. Improving systems of surgical care and drug delivery could substantially reduce the burden of iatrogenic injury.
A Note on the Proprietary and Entity Perspectives in Financial Statements: The Implications for two Current Controversial Issues

Abstract Financial statements can portray the financial position and performance of an entity from different perspectives. Two dominant perspectives are the proprietary and entity perspectives. These perspectives also feature in recent discussions by the IASB and the FASB in relation to their conceptual framework project. The adopted perspective will yield different presentations for a number of issues. This paper illustrates the implications for two controversial issues currently under discussion by the IASB and the FASB: accounting for changes in a reporting entity’s own credit risk when liabilities are measured at fair value, and the classification of certain obligations as either equity or liabilities. The paper explains why the adoption and consistent application of one perspective are important for standard setting and financial reporting to ensure the consistent presentation of an entity’s performance and financial position that can be correctly interpreted by users of financial statements against the background of the chosen perspective.
Munc18-1 binds directly to the neuronal SNARE complex

Both SM proteins (for Sec1/Munc18-like proteins) and SNARE proteins (for soluble NSF-attachment protein receptors) are essential for intracellular membrane fusion, but the general mechanism of coupling between their functions is unclear, in part because diverse SM protein/SNARE binding modes have been described. During synaptic vesicle exocytosis, the SM protein Munc18-1 is known to bind tightly to the SNARE protein syntaxin-1, but only when syntaxin-1 is in a closed conformation that is incompatible with SNARE complex formation. We now show that Munc18-1 also binds tightly to assembled SNARE complexes containing syntaxin-1. The newly discovered Munc18-1/SNARE complex interaction involves contacts of Munc18-1 with the N-terminal Habc domain of syntaxin-1 and the four-helical bundle of the assembled SNARE complex. Together with earlier studies, our results suggest that binding of Munc18-1 to closed syntaxin-1 is a specialization that evolved to meet the strict regulatory requirements of neuronal exocytosis, whereas binding of Munc18-1 to assembled SNARE complexes reflects a general function of SM proteins involved in executing membrane fusion.
An Empirical Study on the Relationship between Financial Development, Economic Growth and Income Disparity in China

Urban and rural development and reduce urban-rural income gap is a hot topic of economic research, interaction between financial development, economic growth and income inequality mechanism of great significance for the study of the problem. In this paper, by constructing the vector error correction model(VEC), and makes an empirical study on the relationship between financial development, economic growth and income gap in China from 1990 to 2016. The study found that financial development, economic growth and income gap have the long-term equilibrium relationship; financial development will expand the income gap, and financial development is resulted widening income gap of Granger reasons, the expansion of the income gap to a certain extent, inhibited the financial development, however, this inhibition is lagging; economic growth has widened the income gap, and the income gap is the reason for the economic growth of Granger, Indicating that the income gap contributed to economic growth; economic growth in the early inhibition of financial development, but in the long run, economic growth to promote financial development, and economic growth is the financial development of Granger reasons.
The tourist experience of domestic VFR travellers: the case of Hungary

Visiting friends and relatives (VFR) is the main driver of domestic travel in many countries. This study is one of the first to analyse VFR travel from a perspective of tourist experience. This study aims to investigate the complex dynamics behind VFR-related travel, especially in aspects of trip activities and tourist experience formation. Based on a survey (N = 879) examining domestic VFR travellers’ trip activities in Hungary, the paper discusses the formation of a tourist experience in context of short-haul domestic VFR travel, especially the role of influencing factors. The results reveal differences between the segments with trip motivation of visiting friends (VF) and visiting relatives (VR) – the two main types of VFR tourists. It was found that independent variables such as VF, staying for longer and participating in leisure and tourism-type activities have a positive effect on tourist experience evolvement; however, factors such as VR, being rather passive during such a visit and focusing on social acts and bonding negatively affect the formulation of a tourist experience. The paper’s novelty and uniqueness lies in applying a new perspective of analysing and discussing VFR travel: the theoretical concept of tourist experience formation.
Comparison of PBDE congeners as inducers of oxidative stress in zebrafish

A proposed primary pathway through which polybrominated diphenyl ethers (PBDEs) disrupt normal biological functions is oxidative stress. In the present study, 4 PBDE congeners were evaluated for their potential to initiate oxidative stress in zebrafish during development: BDE 28, BDE 47, BDE 99, and BDE 100. N‐acetylcysteine (NAC) was used to increase intracellular glutathione concentrations and only decreased the effects of BDE 28 at 10 ppm and 20 ppm and BDE 47 at 20 ppm. N‐acetylcysteine coexposure did not alter the rates of mortality or curved body axis compared with PBDE exposure alone. The activity of glutathione‐S‐transferase (GST) was not altered at 24 h postfertilization (hpf), but increased following 10 ppm BDE 28 exposure at 120 hpf. Transcription of several genes associated with stress was also evaluated. At 24 hpf, cytochrome c oxidase subunit 6a (COX6a) transcription was up‐regulated in embryos exposed to BDE 99, and BDE 28 exposure up‐regulated the transcription of Glutathione‐S‐transferase‐pi (GSTpi). At 24 hpf, glutamate‐cysteine ligase (GCLC) was slightly down‐regulated by all congeners evaluated. At 120 hpf, TNF receptor‐associated protein 1 (TRAP1) and COX6A were up‐regulated by all congeners, however GSTpi was down‐regulated by all congeners. The results of quantitative real‐time transcription polymerase chain reaction are mixed and do not strongly support a transcriptional response to oxidative stress. According to the authors' data, PBDEs do not induce oxidative stress. Oxidative stress may occur at high exposure concentrations; however, this does not appear to be a primary mechanism of action for the PBDE congeners tested. Environ Toxicol Chem 2015;34:1154–1160. © 2015 SETAC
Hostile, Hypoxia–A2-Adenosinergic Tumor Biology as the Next Barrier to Overcome for Tumor Immunologists

Hypoxia-driven, A2A adenosine receptor (A2AR)–mediated (hypoxia–A2-adenosinergic), T-cell–autonomous immunosuppression was first recognized as critical and nonredundant in protecting normal tissues from inflammatory damage and autoimmunity. However, this immunosuppressive mechanism can be highjacked by bacteria and tumors to provide misguided protection for pathogens and cancerous tissues. Inhibitors of the hypoxia–A2-adenosinergic pathway represent a conceptually novel type of immunologic coadjuvants that could be combined with cancer vaccines, adoptive cell transfer, and/or blockade of negative immunologic regulators to further prolong patient survival and to minimize treatment-related side effects. In support of this approach are preclinical studies and findings that some human cancers are resistant to chemotherapies and immunotherapies due to the tumor-generated extracellular adenosine and A2AR on antitumor T and natural killer (NK) cells. Among the coadjuvants are (i) antagonists of A2AR, (ii) extracellular adenosine-degrading drugs, (iii) inhibitors of adenosine generation by CD39/CD73 ectoenzymes, and (iv) inhibitors of hypoxia–HIF-1α signaling. Combining these coadjuvants with CTLA-4 and/or PD-1 blockade is expected to have additive or even synergistic effects of targeting two different antitumor protective mechanisms. It is expected that even after multicombinatorial blockade of negative immunologic regulators, the antitumor T and NK cells would still be vulnerable to inhibition by hypoxia and A2AR. Yet to be tested is the potential capacity of coadjuvants to minimize the side effects of CTLA-4 and/or PD-1 blockade by decreasing the dose of blocking antibodies or by eliminating the need for dual blockade. Cancer Immunol Res; 2(7); 598–605. ©2014 AACR.
RhoB is required to mediate apoptosis in neoplastically transformed cells after DNA damage

The effect of neoplastic transformation on the response to genotoxic stress is of significant clinical interest. In this study, we offer genetic evidence that the apoptotic response of neoplastically transformed cells to DNA damage requires RhoB, a member of the Rho family of actin cytoskeletal regulators. Targeted deletion of the rhoB gene did not affect cell cycle arrest in either normal or transformed cells after exposure to doxorubicin or gamma irradiation, but rendered transformed cells resistant to apoptosis. This effect was specific insofar as rhoB deletion did not affect apoptotic susceptibility to agents that do not damage DNA. However, rhoB deletion also affected apoptotic susceptibility to Taxol, an agent that disrupts microtubule dynamics. We have demonstrated that RhoB alteration mediates the proapoptotic and antineoplastic effects of farnesyltransferase inhibitors, and we show here that RhoB alteration is also crucial for farnesyltransferase inhibitors to sensitize neoplastic cells to DNA damage-induced cell death. We found RhoB to be an important determinant of long-term survival in vitro and tumor response in vivo after gamma irradiation. Our findings identify a pivotal role for RhoB in the apoptotic response of neoplastic cells to DNA damage at a novel regulatory point that may involve the actin cytoskeleton.
Aggressive Behavior of Stage I Ovarian Mucinous Tumors Lacking Extensive Infiltrative Invasion: A Report of Four Cases and Review of the Literature

Previous studies have indicated that mucinous carcinomas of the ovary associated with extraovarian spread at the time of presentation or follow-up almost always have extensive infiltrative invasion within the primary tumor. We present four cases of stage I ovarian mucinous tumors that lacked extensive infiltrative invasion but were associated with an unexpectedly aggressive behavior. The patients were 18, 20, 41, and 45 years of age at presentation. All four of them presented with an abdominal mass or increased abdominal girth. The tumors were all stage Ia, 17 to 37 centimeters in maximal dimension, and typically multicystic with solid areas. The number of blocks per centimeter of tumor diameter was 0.65, 0.88, 0.92, and 1.0 in the four tumors, respectively, a degree of sampling within that recommended in previous studies. Clinical findings supported that they were primary tumors rather than metastatic from an occult primary tumor. On microscopic examination, the tumors all contained foci of intraepithelial carcinoma and foci of invasion as follows: expansile invasion only (two cases), expansile invasion and microinvasive carcinoma (one case), and microinvasive carcinoma only (one case). The expansile invasion was extensive in each of the three cases in which it was present. On follow-up, each patient experienced recurrent disease 7 months to 4.5 years after diagnosis, including hematogenous spread to lung and/or bone and liver in three patients. Three of four patients developed intraperitoneal spread. Three of four patients died of disease, and one patient is alive with persistent disease. Although ovarian mucinous tumors with only expansile invasion or only microinvasive carcinoma are usually associated with an excellent prognosis, this study indicates that these tumors can rarely behave in an aggressive fashion with hematogenous spread and a fatal outcome. Some of these tumors may have contained unsampled foci of infiltrative invasion. Although the optimum level of sampling in mucinous tumors remains to be determined, we recommend additional sampling of tumors in which the initial sections reveal intraepithelial carcinoma, microinvasive carcinoma, expansile invasion, or combinations thereof.
Latvia tax review

Latvia's Ministry of Finance requested the World Bank to collaborate on a review of the country's tax system as input for the design a medium-term tax strategy. The motivation behind the tax review is to find options to increase tax revenues by three percentage points of GDP to reach a target tax-to-GDP ratio of 33 percent in the medium term. In Latvia, tax revenues are lower than predicted for its income level and institutional development. The additional revenues would be used to cover growing spending needs in the following areas: Defense pending. The Government aims to increase defense spending to the NATO guideline target of 2 percent of GDP by 2018 (from 1 percent of GDP in 2015 and an estimated 1.5 percent of GDP in 2016). Investment in human capital. Health, education and social protection are additional areas that the Government has identified as requiring further investments. In particular, public health spending is low and health outcomes are lagging compared with much of the European Union (EU).
Test-Retest Reliability of the Eurofit Test Battery Administered to University Students

The purpose of this study was to examine the rest-retest reliability of the Eurofit motor fitness tests performed by university students. A total of 98 undergraduate students who were enrolled in physical education departments in Greece participated (29 men aged 19.5 ± 2.7 hr. and 66 women aged 19, 4 ± 2.7 yr.). ALL Eurofit motor fitness tests and anthropometric measurements were obtained twice with one week between the two measurements, Intraclass correlation coefficient indicated satisfactory coefficients above . 70 for most tests. The only exception was the plate-tapping test which yielded a low value (R = .57). Further, the majority of the Eurofit test battery fitted well within the 95% confidence interval, and only three Eurofit motor fitness test items (flamingo balance, plate tapping, and sit-ups) presented a confidence limit below the value of . 70, These findings indicated that the Eurofit test battery yielded reliable data for undergraduate students. However, modifications should be considered to improve the reliability of certain test items, for application to undergraduates.