Miracle-dz/UniFAR-FTUs huggingface dataset:
The Phenomenology of Shame: A Qualitative Study

Shame is one of the emotions that a person experiences in a variety of everyday situations and many cases it is annoying. Shame is known as a moral emotion, yet its role in psychopathology has been emphasized. This study aimed to examine the lived experience of shame in individuals. This research is a qualitative study with a phenomenological approach. This research describes in-depth what shame is and how it is experienced from the perspective of individuals. The participants included eight men and seven women who contributed to an in-depth unstructured interview. A seven-step Colaizzi method was used to analyze the data. Data were analyzed using MAXQDA (2018) software.Based on our findings, the eight themes of "physical reactions", "accompanying emotions", "making mistakes", "vicarious shame", "gaze of others", "being subject to judgment", " preoccupation" and three sub-themes of "worry", "rumination" and "blame", "existential shame" with the sub-themes of "inadequacy" and "feeling different" are the most common ones in people's experience of shame. Results are discussed regarding the existing literature. In general, the study of people's experience of shame shows that there are common themes in the description of different people from what they have experienced. The use of shame as a concept in psychotherapy may improve our understanding of the nature of some psychological problems.
Reduced mitochondrial lipid oxidation leads to fat accumulation in myosteatosis

Myosteatosis is the pathological accumulation of lipid that can occur in conjunction with atrophy and fibrosis following skeletal muscle injury. Little is known about the mechanisms by which lipid accumulates in myosteatosis, but many clinical studies have demonstrated the degree of lipid infiltration negatively correlates with muscle function and regeneration. Our objective was to determine the pathological changes that result in lipid accumulation in injured muscle fibers. We used a rat model of rotator cuff injury in this study, as the rotator cuff muscle group is particularly prone to the development of myosteatosis after injury. Muscles were collected from uninjured controls, or 10, 30, or 60 days after injury, and analyzed using a combination of muscle fiber contractility assessments, RNA sequencing, and undirected metabolomics, lipidomics and proteomics, along with bioinformatics techniques, to identify potential pathways and cellular processes that are dysregulated after rotator cuff tear. Bioinformatics analyses indicated that mitochondrial function was likely disrupted after injury. Based on these findings, and given the role that mitochondria play in lipid metabolism, we then performed targeted biochemical and imaging studies and determined that mitochondrial dysfunction and reduced fatty acid oxidation likely leads to the accumulation of lipid in myosteatosis.
Analysis of Reaction-Transport Phenomena in a Microfluidic System for the Detection of IgG*

A spatially two-dimensional mathematical model of microfluidic biosensor for immunological determination of human immunoglobulin G (IgG) with the use of Protein A (PA) immobilized on the internal walls of a microchannel is presented. Convection flow in the microdevice is induced by an imposed difference of electric potential (electroosmosis). In the model, the electroosmotic convection is described using the slip boundary conditions that can be defined by the Helmholtz—Smoluchowski equation. Incubation phase (formation of the immobile PA—IgG complex) of the immunoassay has been studied. Effects of the antibody concentration in a sample, the imposed difference of electric potential, the surface heterogeneities in the reaction zone, and other model parameters on the saturation time were determined. It was found that the surface heterogeneities could form complex velocity fields at the location of the adsorption zone: either an intensive flow at the microchannel walls or the nozzle-like flow. Generally, the local acceleration of the flow causes the decrease of the mass-transfer resistance. Further, imposed electric field of a proper orientation was able to shorten the incubation phase to 600 s, assuming the microchannel device with the diameter of 100 µm and the chosen reaction kinetics. Hence, the incubation phase could be substantially reduced enabling, e.g., fast diagnostics. Simulation of the effects of the antibody sample concentration revealed good qualitative agreement with experimental data obtained in a similar microfluidic device (published by Dodge et al., Anal. Chem. 73, 3400 (2001)).
A REVIEW ON RECENT TRENDS OF ULTRASOUND ASSISTED PROCESSING IN FOOD SEGMENT

In the food industry, ultrasound is a recent and potentially favorable technology. A number of physical and chemical changes are generated in the food by the transmission and distribution of ultrasound waves which enhance the productivity of many food processing operations. This technology is also used as a diagnostic tool in quality control of food products. Ultrasound processing falls under the category of green technology due to ecofriendly and non toxic behavior.The present review summarizes the role of this ultrasound technology along with its aids to the industry for example; improvements in cooking quality, enhancements of physicochemical properties, microbial inactivation, and also pretreatment to reduce acrylamide and to recover the valuable biomaterial from the food waste.
Role of miR156 and miR171 in drought response of tobacco

microRNAs (miRNAs) are genetic regulator RNA molecules belonging to small RNA family. Functions of miRNAs in plants range in a broad spectrum, one of which is the drought stress that is a major environmental stress factor globally affecting plant growth and development. Overexpression of a gene indicates elevated expression of a certain gene beyond its normal expression level. Studies seeking for function of plant miRNAs in response to overexpression carries significant role for agricultural improvement. Among wide variety of plant miRNAs with diverse functions, miR156 and miR171 have critical roles on plant development and stress tolerance. In the first part of our study, different Turkish tobacco cultivars were subjected to drought stress and RNA from leaf tissue of these plants was utilized for changes of gene expression levels in miR156 and miR171. In the second part of project, leaf explants of grown tobacco cultivars were transformed with Agrobacterium tumefaciens containing MIR156 and MIR171 genes of tobacco cultivars and successful overexpressed putative transgenic plants were selected. Callus tissue formed from putative transgenic and control leaf explants groups were also subjected to drought stress and drought stress response of miR156 and miR171 expression levels were analyzed by quantitative real time PCR. Scope of this study could be utilized for a better understanding of molecular mechanisms of drought stress and the roles of miRNAs.
RMI-chip: a microfluidics setup for functional imaging of microbial interactions with tree roots

Coupling microfludics with microscopy has emerged as a powerful approach to study dynamics in plant physiology at cellular resolution and at higher throughput than with conventional methods. Most devices have been designed to study the model plant Arabidopsis thaliana, including root-microbe interactions. However, there is a need for microfluidic devices which enable in vivo studies of root development and root-microbe interactions in woody plants. Here, we developed the RMI-chip, a microfluidic setup that allows for continuous microscopic scale observation of real-time interactions between live Populus tremuloides (aspen tree) seedling roots and the rhizobacterium Pseudomonas fluorescens over an extended period longer than a month. Colonization dynamics in the RMI-chip are consistent with previous observations in a different experimental set-up. Also, we find that biosensors based on the rhizobacterium Bacillus subtilis can be used to monitor compositional changes in root exudates but that their application is limited by their inefficient colonization of aspen roots. Our results indicate that functional imaging of dynamic root-bacteria interactions in the RMI-chip requires careful matching between the host plant and the bacterial root colonizer.
Glicerol na dieta de tilápias do Nilo (Oreochromis niloticus)

O maior custo de producao em um cultivo de peixes esta relacionado a nutricao dos animais que passa dos 50%, por isso, foram realizados dois experimentos baseados na digestibilidade e inclusao do glicerol para tilapias do Nilo (Oreochromis niloticus). O objetivo deste trabalho foi avaliar a digestibilidade aparente de tres fontes de glicerois, bruto oleo vegetal (GBV), glicerol semi-purificado misto (GSM) e glicerol semi-purificado vegetal (GSV) (experimento 1) e, posteriormente determinar ate que nivel de inclusao esse alimento pode ser utilizado em dietas para tilapias e se o mesmo provoca alteracoes nas caracteristicas bioquimicas do sangue e na composicao centesimal dos animais (experimento 2). No experimento 1, utilizou-se 40 peixes com peso medio de 343,12 ± 77,71 g, distribuidos em quatro tanques de 180 litros com fundo conico, num delineamento experimental em quadrado latino. Foram confeccionadas quatro dietas experimentais, sendo uma referencia e tres testes, compostas com a inclusao de 20% do alimento teste e 80% da dieta referencia. Como indicador foi utilizado o oxido de cromioIII (0,1% da dieta). Foram obtidos valores de energia digestivel do glicerol bruto oleo vegetal (GBV), glicerol semi-purificado misto (GSM) e glicerol semi-purificado vegetal (GSV), de 3058,55; 2610,55; 1754,70 kcal.kg-1, respectivamente. Para o experimento 2, utilizou-se o glicerol que possuia maior quantidade de energia digestivel (GBV) e confeccionou-se cinco racoes, contendo niveis crescentes de glicerol (0,0; 2,5; 5,0; 7,5 e 10%), por um periodo de 79 dias e fornecidas a 300 juvenis de tilapia do Nilo com peso medio de 29,15 ± 8,40 g e 11,55 ± 0,87 cm de comprimento. Apos esse periodo foi avaliado o desempenho dos animais atraves do peso final (PF), comprimento final (CF), sobrevivencia (SO), ganho de peso (GP), conversao alimentar (CA), taxa de crescimento especifico (TCE) fator de condicao (FC), indice hepatossomatico (IHS) e gordura visceral (GV). Para as avaliacoes dos parâmetros bioquimicos do sangue foram avaliados proteina (PROT), triglicerideos (TRIG), colesterol (COLE), lipoproteina HDL, glicose (GLIC) e lipoproteinas VLDL e LDL. Para a determinacao da composicao centesimal foram avaliadas a umidade, lipideos, proteinas e cinzas. Nao houve diferenca estatistica entre os diferentes tratamentos sobre o desempenho zootecnico dos animais. Para os parâmetros bioquimicos apenas a lipoproteina HDL teve diferenca estatistica (P<0,05), sendo superior no grupo de peixes alimentados com 0,0 e 7,5%, nao diferindo dos animais que foram alimentados com 5,0 e 10,0%. Para a composicao centesimal dos juvenis de tilapia foi verificada diferenca na quantidade de lipideos (P<0,05), que tiveram os maiores valores nos peixes alimentados com 5,0% de glicerol. Pode ser incluso 10% do alimento que nao ocorre prejuizos zootecnicos nem qualquer dano a saude dos animais.
Red de comunicación para mensajería sin cobertura de Internet o red móvil a través de dispositivos móviles

Se describe el proceso para el intercambio de informacion entre Bluetooth y LoRa para la creacion de un canal que permita la comunicacion desde un dispositivo movil sin la cobertura de red celular. Se muestra el diseno del circuito electronico y de una carcasa para la portabilidad del prototipo, asi como su montaje final. Se analiza la teoria sobre LoRa por medio de experimentos que llevan a definir los alcances que tiene al ser expuesto a distintos ambientes. Se exponen resultados de pruebas de cobertura, realizadas en un escenario real con caracteristicas del protocolo especificas, incluyendo un analisis de perdidas de mensajes con el cual se estima la confiabilidad de la red.
Early Brain Imaging can Predict Autism: Application of Machine Learning to a Clinical Imaging Archive

A comprehensive investigation of early brain alterations in Autism Spectrum Disorder (ASD) is critical for understanding the neuroanatomical underpinnings of autism and its early diagnosis. Most previous brain imaging studies in ASD, however, are based on children older than 6 years – well after the average age of ASD diagnosis (~46 months). In this study, we use brain magnetic resonance images that were collected as part of clinical routine from patients who were later diagnosed with ASD. Using 15 ASD subjects of age three to four years and 18 age-matched non-ASD subjects as controls, we perform comprehensive comparison of different brain morphometric features and ASD vs. non-ASD classification by Random Forest machine learning method. We find that, although total intracranial volume (TIV) of ASD was 5.5 % larger than in non-ASD, brain volumes of many other brain areas (as a percentage of TIV) were smaller in ASD and can be partly attributed to larger (>10 %) ventricles in ASD. The larger TIV in ASD was correlated to larger surface area and increased amount of cortical folding but not to cortical thickness. The white matter regions in ASD had less image intensity (predominantly in the frontal and temporal regions) suggesting myelination deficit. We achieved 95 % area under the ROC curve (AUC) for ASD vs. non-ASD classification using all brain features. When classification was performed separately for each feature type, image intensity yielded the highest predictive power (95 % AUC), followed by cortical folding index (69 %), cortical and subcortical volume (69 %), and surface area (68 %). The most important feature for classification was white matter intensity surrounding the rostral middle frontal gyrus and was lower in ASD (d = 0.77, p = 0.04). The high degree of classification success indicates that the application of machine learning methods on brain features holds promise for earlier identification of ASD. To our knowledge this is the first study to leverage a clinical imaging archive to investigate early brain markers in ASD.
Mécanismes d'infection de l'ectoparasite de poissons d'eau douce Tracheliastes polycolpus

Quels mecanismes ecologiques et evolutifs rendent les interactions entre especes possibles ? J'ai aborde cette question en considerant l'interaction entre un parasite de poisson d'eau douce (Tracheliastes polycolpus) et plusieurs de ses hotes cyprinides. J'ai d'abord mis en avant le role de l'environnement a une echelle spatiale tres fine (quelques centaines de metres) sur le taux de rencontre entre ce parasite et ses hotes. A plus large echelle spatiale (la France) j'ai illustre le role limitant de la resistance des populations hotes sur la distribution de T. polycolpus. Par ailleurs j'ai montre que T. polycolpus parvient a parasiter differentes especes hotes en s'appuyant sur une expression plastique de ses genes. Enfin, j'ai montre que les communautes bacteriennes associees a l'hote changent consecutivement a l'infection parasitaire dans une dynamique de co-infection avec le parasite. Par le deploiement d'approches variees et complementaires, il apparait que des facteurs environnementaux, lies a l'hote, au parasite ou encore a leur cortege de bacteries contribuent a la realisation d'une interaction etroite et durable tel que le parasitisme.
Immune profiling in M. tuberculosis infection enables stratification of patients with active disease

Tuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb) infection and is a major public health problem with an estimated 1.7 billion persons infected worldwide. Clinical challenges in TB include the lack of a blood-based test for active disease, and the absence of prognostic biomarkers for early treatment response. Current blood based tests, such as QuantiFERON-TB Gold (QFT), are based on an IFNγ readout following Mtb antigen stimulation. However, they do not distinguish active TB disease from asymptomatic Mtb infection. We hypothesized that the use of TruCulture, an improved immunomonitoring method for whole blood collection and immune stimulation, could improve the discrimination of active disease from latent Mtb infection. To test our hypothesis, we stimulated whole blood from active TB patients (before and after successful treatment), comparing them to asymptomatic latently infected individuals. Mtb-specific antigens (ESAT-6, CFP-10, TB7.7) and live bacillus Calmette-Guerin (BCG) were used for TruCulture stimulation conditions, with direct comparison to QFT. Protein analyses were performed on the culture supernatants using ELISA and Luminex multi-analyte profiling. TruCulture showed an ability to discriminate active TB cases from latent controls (p < 0.0001, AUC = 0.81, 95% CI: 0.69-0.93) as compared to QFT (p = 0.47 AUC = 0.56, 95% CI: 0.40-0.72), based on an IFNγ readout after Mtb antigen stimulation. The stratification of the two groups could be further improved by using the Mtb Ag/BCG IFNγ ratio response (p < 0.0001, AUC = 0.918, 95% CI: 0.84-0.98). We also identified additional cytokines that distinguished latent infection from TB disease; and show that the primary differences between the TruCulture and QFT systems were a result of higher levels of non-specific innate immune activation in QFT tubes, due to the lack of a buffering solution in the latter. We conclude that TruCulture offers a next-generation solution for whole blood stimulation and immunomonitoring with the possibility to discriminate active and latently infected persons.
Incompleteness for stably sound Turing machines

We first partly develop a mathematical notion of stable soundness intended to reflect the actual soundness property of human beings. Then we show that given an abstract query machine $M$ (a function) the following cannot hold simultaneously: $M$ is stably sound, $M$ is computable, $M$ can decide the truth of any arithmetic statement. This can be understood as an extension of the G\"odel incompleteness theorem to stably sound setting. This is a non-trivial extension as a stably sound Turing machine can decide the halting problem. In practice such an $M$ could be meant to represent a weakly idealized human being so that the above gives an obstruction to computability of intelligence, and this gives a formal extension of a famous disjunction of G\"odel.
Method of social network analysis and visualisation based on reviews

With the development of e-commerce platforms, the huge amount of transaction volume produces a lot of commentary information. To study the relationship between users and user comments and corresponding products, a social network analysis method based on user comments is proposed. The authors regard the network connection between goods and user comments as a problem on the network and use the spectral clustering algorithm to maintain the structural characteristics of the network. This method can visualise the network structure features such as user clustering and distribution, which can help to understand the network from a macro perspective and be directly applied to the visualisation of other comment-based networks. It is significant for analysing the relationship between users and user comments and corresponding products. The comparative visualisation experiments are also discussed, to validate the effectiveness of the proposed method.
Raffia palm (Raphia hookeri) wine extenuates redox imbalance and modulates activities of glycolytic and cholinergic enzymes in hyperglycemia-induced testicular injury in type 2 diabetic rats.

This study investigated the effects of Raffia palm wine (RPW) on redox imbalance, glycolytic and cholinergic enzymes, and ATPase activities in hyperglycemia-induced oxidative testicular injury. Type 2 diabetes (T2D) was induced in male albino rats (Sprague-Dawley) by first administering 10% fructose solution for 14 days, before injecting with an intraperitoneal injection (40 mg/kg body weight) of streptozotocin. Raffia palm wine was administered to two diabetic groups at 150 and 300 mg/kg body weight (bw), when untreated diabetic group was used as a negative control, and metformin-fed group was served as a positive control. The rats were sacrificed after 5 weeks of treatment, and testes were harvested. Treatment with RPW led to lower levels of nitric oxide, malondialdehyde and myeloperoxidase activity, with concomitant elevation of reduced glutathione level, superoxide dismutase, catalase and ATPase activities. Raffia palm wine also inhibited glycogen phosphorylase, glucose-6-phosphatase, fructose-1,6-bisphosphatase, and acetylcholinesterase, while restoring the altered testicular morphology to near-normal. The results of this study suggest the therapeutic potentials of RPW against the effects of T2D on testicular functions and morphology. PRACTICAL APPLICATIONS: Diabetes mellitus is one of the fastest growing global epidemy, with most developing countries being at the receiving end owing to the cost of treatment. Testicular damage has been recognized as one of the complications of diabetes, and if left untreated will lead to erectile dysfunction followed by infertility. Raffia palm wine is among the common natural beverage in West Africa, which is utilized for both social and medicinal purposes. In this study, for the first time we showed its ability to protect diabetes-induced testicular injury in type 2 diabetic rats. This may be of great benefit in managing diabetes associated erectly dysfunction and male infertility, as Raffia palm wine is readily available in all seasons. This study will also improve the medicinal use of this wine, which may also indirectly improve its commercial benefit.