Miracle-dz/UniFAR-FTUs huggingface dataset:
Elastic sheet on a liquid drop reveals wrinkling and crumpling as distinct symmetry-breaking instabilities

Smooth wrinkles and sharply crumpled regions are familiar motifs in biological or synthetic sheets, such as rapidly growing plant leaves and crushed foils. Previous studies have addressed both morphological types, but the generic route whereby a featureless sheet develops a complex shape remains elusive. Here we show that this route proceeds through an unusual sequence of distinct symmetry-breaking instabilities. The object of our study is an ultrathin circular sheet stretched over a liquid drop. As the curvature is gradually increased, the surface tension stretching the sheet over the drop causes compression along circles of latitude. The compression is relieved first by a transition into a wrinkle pattern, and then into a crumpled state via a continuous transition. Our data provide conclusive evidence that wrinkle patterns in highly bendable sheets are not described by classical buckling methods, but rather by a theory which assumes that wrinkles completely relax the compressive stress. With this understanding we recognize the observed sequence of transitions as distinct symmetry breakings of the shape and the stress field. The axial symmetry of the shape is broken upon wrinkling but the underlying stress field preserves this symmetry. Thus, the wrinkle-to-crumple transition marks symmetry-breaking of the stress in highly bendable sheets. By contrast, other instabilities of sheets, such as blistering and cracking, break the homogeneity of shape and stress simultaneously. The onset of crumpling occurs when the wrinkle pattern grows to half the sheet’s radius, suggesting a geometric, material-independent origin for this transition.
Activity analysis of English premiership rugby football union refereeing

Little is known about the physiological demands placed on officials during sporting activities. The purpose of this study was to ascertain the movement activities of referees during English Premiership rugby football union matches, and to determine the frequency and duration of these activities. Nine referees who were ranked in the top 20 referees in England were videotaped during a total of 19 matches. During playback of the videotapes, a single observer coded the referees' activities into one of six distinct categories (standing, walking forwards, walking backwards, jogging, running and sprinting) using a computerized video editing system (Observer Video-Pro). The referees were timed over a 20 m distance for the modes of locomotory activity, and the average velocity of the referee for each activity was used to calculate the total distance covered in each mode of activity during matches. The total distance covered during a match was (χ ± SD) 8581 ± 668 m. The mean percentage of total playing time spent in each activity was: standing still, 37.0 ± 11.0%; walking forward, 29.5 ± 7.2%; walking backward, 9.9 ± 3.2%; jogging, 12.8 ± 3.2%; running, 9.8 ± 2.3%; and sprinting, 1.0 ± 0.4%. There were a total of 672 transitions between modes of activity during a match. The results of this study suggest that refereeing top English rugby football union matches is physically demanding. Although the major physiological load is placed on the oxygen transport system, frequent sprint bouts and the associated requirements for acceleration and deceleration impose additional metabolic demands on referees. This information may be used in the design of physical training programmes to optimize performance in referees.
Cerebrospinal fluid p24 antigen levels and intrathecal immunoglobulin G synthesis are associated with cognitive disease severity in HIV‐1

ObjectiveTo test the hypothesis that selected cerebrospinal fluid (CSF) markers [intrathecal immunoglobulin G (IgC) synthesis rate, oligoclonal IgC bands, and p24 antigen levels] are associated with the presence and severity of clinical HIV-1 neurologic disease. Design and methodsCSF and blood parameters from 142 HIV-seropositive subjects from the baseline examination of a longitudinal study were measured and analyzed in relationship with clinically derived cognitive impairment groups (none, mild, moderate) and with other neurologic and clinical classification groups. Subjects with opportunistic infections, lymphomas or neurosyphilis were excluded. ResultsThe mean intrathecal IgG synthesis rate and mean CSF p24 antigen levels both differed significantly among cognitive impairment groups; more impairment was associated with a higher rate or level. Mean CSF p24 antigen levels were significantly higher in HIV-1-seropositive subjects with any HIV-1 neurologic disease than in subjects without neurologic disease. In contrast, there were no significant differences among seropositive groups in any CSF parameter when stratified by systemic disease classification (asymptomatic HIV-seropositives, AIDS-related complex, or AIDS), independent of neurologic status. ConclusionWe conclude that there may be a relationship between the severity of HIV cognitive disease and increasing levels of intrathecal IgC synthesis and CSF p24 antigen levels.
A role for novel lipid interactions in the dynamic recruitment of SNX27 to the T-cell immune synapse

SNX27 is a member of the sorting nexin family that plays an important role in the recycling of receptors from endosomes to the cell surface. In addition to a PX (Phox homology) domain that regulates its endosomal localization, SNX27 has a unique PDZ (Psd-95/Dlg/ZO1) domain and an atypical FERM (4.1, ezrin, radixin, moesin) domain that both function to bind short peptide sequence motifs in the cytoplasmic domains of the cargo receptors. Using the T cell immune synapse (IS) as a model for polarized protein recycling, we recently identified an additional mechanism that enhances SNX27 localization to the endosomal recycling compartment (ERC). Our study defined a phosphoinositide (PI) lipid-binding site within the SNX27 FERM domain, with a clear preference for bi- and triphosphorylated PIs, which may promote SNX27 localization to phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) and/or PtdIns(3,4,5)P3-enriched membrane domains. Using fluorescently tagged lipid-binding probes, we studied the kinetics of distinct PIs in living T cells during IS formation. Our results suggest that PtdIns(3,4,5)P3 accumulates at the contact site simultaneously with early SNX27 recruitment to the plasma membrane (PM), and this is partly controlled by by lipid binding through the FERM domain. These studies define 2 independent binding sites for PtdIns-derived lipids in SNX27, that contribute to the dynamic recruitment of SNX27 to distinct membranes during T cell activation.
Cortical thickness and subcortical structure volume abnormalities in patients with major depression with and without anxious symptoms

Anxious depression is one of the common subtypes of major depressive disorder (MDD). Clinically, patients with anxious depression exhibit more severe depressive symptoms than patients with nonanxious depression. The aim of the present study was to explore the common and differing cortical and subcortical structural changes between patients with anxious and nonanxious depression.
Digitally produced fiber-reinforced composite substructures for three-unit implant-supported fixed dental prostheses.

PURPOSE This study aimed to evaluate the probability of survival, Weibull modulus, characteristic strength, and failure modes of computer-aided design/computer-assisted manufacture (CAD/CAM) fiber-reinforced composite (FRC) substructures used for implant-supported fixed dental prostheses (ISFDPs). MATERIALS AND METHODS Three-unit ISFDPs (first molar pontic) fabricated as a monolithic composite piece or as composite veneered on a CAD/CAM FRC substructure with either a 12-mm² or 3-mm² connector area (n = 18 each) were subjected to step-stress accelerated life testing in water. Use-level probability Weibull curves and the probability of survival were calculated. Fractographic analysis was performed under polarized light and scanning electron microscopy. RESULTS Fatigue did not accelerate the failure of any group, whereas prosthesis strength was the main factor in increased failure (β < 1). The probability Weibull contour plot showed no differences between the ISFDPs with 12 mm² and the monolithic composite ISFDP in characteristic strength (η = 643.5 N and 742.7 N, respectively) or Weibull modulus (6.7 and 5.8, respectively), whereas both were significantly higher than 3 mm² (444.91 N and 9.57). The probability of survival was not statistically different between groups at 100,000 mission cycles at 300 N. Differences were observed in fatigue failures above 800 N; monolithic composite ISFDPs failed catastrophically, whereas those with CAD/CAM FRC substructures presented veneer/composite cohesive or adhesive failures. Cracks evolved from the occlusal contact toward the margins of the cohesively failed composite, and in CAD/CAM FRC prostheses, competing failure modes of cracks developing at the connector area with those at the indentation contact were observed. CONCLUSION The probability of survival did not differ between CAD/CAM FRC with either 3-mm² or 12-mm² connector areas, monolithic composite, or metal-ceramic ISFDPs previously tested under the same methodology. However, differences in failure modes were detected between groups.
Prediction of Response to Electroconvulsive Therapy

In an open study of 35 depressed patients, psychomotor disturbance, assessed by the CORE rating system, significantly predicted response to ECT in patients with a ‘retarded’ but not those with an ‘agitated’ type of illness. In multivariate analyses, psychomotor disturbance was the sole predictor of response. This preliminary study suggests that the CORE system, which has been shown to differentiate endogenous and non-endogenous depressive disorders, has predictive validity in relation to ECT response.
High-Frequency Thin-Film AlN-on-Diamond Lateral–Extensional Resonators

In this paper, low-impedance lateral-extensional microresonators are fabricated on a stack of aluminum nitride (AlN) directly deposited on a polished ultrananocrystalline diamond (UNCD) film. The large acoustic velocity of UNCD is utilized to extend the frequency of such resonators beyond 1 GHz while the frequency-defining features are not reduced excessively. In order to promote the growth of a c-plane piezoelectric AlN film, the surface of the UNCD film is polished after deposition. Three different UNCD films with different Young's modulus values were prepared, and frequencies up to two times that of similar devices fabricated on silicon have been achieved. The finite-element analysis is employed to evaluate the effect of various physical parameters on the performance of the thin-film piezoelectric-on-substrate resonators in order to achieve very low motional resistance (Rm). Several resonators were designed with various lateral dimensions and different numbers of support tethers to evaluate the propositions. The lowest Rm was measured from a multitethered 29th-order thin-film piezoelectric-on-diamond (TPoD) resonator (22 Ω) and f · Q product of 2.72 * 1012 at 888 MHz. The temperature coefficient of frequency of this TPoD resonator is measured to be -9.6 ppm/°C, which is much lower than that of the devices fabricated on silicon. Also, this device can withstand input powers up to +27 dBm, leading to a delivered power density per unit area of ~2.9 μW/μm2.
Clinical and immunological characteristics of elderly onset Sjögren's syndrome: a comparison with younger onset disease.

OBJECTIVE To compare the clinical and laboratory characteristics of patients with primary Sjögren's syndrome (SS) with an elderly onset to those with a younger onset. METHODS The study group comprised 85 consecutive patients (79 women and 6 men) attending the Sjögren's clinic. Primary SS was diagnosed according to the San Diego criteria. Elderly onset disease (EOD) was determined as the appearance of symptoms suggestive of SS after age 65. Clinical and laboratory variables for EOD were compared to those of a younger onset disease (YOD). Salivary and serum samples of all patients were examined for concentrations of interleukin 6 (IL-6) and hyaluronic acid (HA). RESULTS Seventeen patients with SS (20%) matched the definition of EOD and their median disease onset was 71 years (range 65-80). No significant differences were noted in the clinical disease manifestations between the 2 groups of patients. Rheumatoid factor and anti-Ro(SSA) antibodies were more common in the YOD group (p = 0.012 and p = 0.023, respectively). Significant elevations of salivary IL-6 and HA levels were detected in the YOD group compared to the EOD group with SS (17.3 +/- 3.6 vs 8.8 +/- 2.1 pg/ml and 230.2 +/- 41.1 vs 128.8 +/- 33.3 ng/ml, respectively) (p < 0.0001). CONCLUSION EOD SS has somewhat milder clinical symptoms with fewer immunological manifestations than YOD. The elevations of salivary IL-6 and HA in the younger group of SS patients support in part the differences in the inflammatory process between the 2 groups.
Characterization, Ecological Distribution, and Population Dynamics of Saccharomyces Sensu Stricto Killer Yeasts in the Spontaneous Grape Must Fermentations of Southwestern Spain

ABSTRACT Killer yeasts secrete protein toxins that are lethal to sensitive strains of the same or related yeast species. Among the four types of Saccharomyces killer yeasts already described (K1, K2, K28, and Klus), we found K2 and Klus killer yeasts in spontaneous wine fermentations from southwestern Spain. Both phenotypes were encoded by medium-size double-stranded RNA (dsRNA) viruses, Saccharomyces cerevisiae virus (ScV)-M2 and ScV-Mlus, whose genome sizes ranged from 1.3 to 1.75 kb and from 2.1 to 2.3 kb, respectively. The K2 yeasts were found in all the wine-producing subareas for all the vintages analyzed, while the Klus yeasts were found in the warmer subareas and mostly in the warmer ripening/harvest seasons. The middle-size isotypes of the M2 dsRNA were the most frequent among K2 yeasts, probably because they encoded the most intense K2 killer phenotype. However, the smallest isotype of the Mlus dsRNA was the most frequent for Klus yeasts, although it encoded the least intense Klus killer phenotype. The killer yeasts were present in most (59.5%) spontaneous fermentations. Most were K2, with Klus being the minority. The proportion of killer yeasts increased during fermentation, while the proportion of sensitive yeasts decreased. The fermentation speed, malic acid, and wine organoleptic quality decreased in those fermentations where the killer yeasts replaced at least 15% of a dominant population of sensitive yeasts, while volatile acidity and lactic acid increased, and the amount of bacteria in the tumultuous and the end fermentation stages also increased in an unusual way.
Ciliary Neurotrophic Factor Expression in Schwann Cells Is Induced by Axonal Contact

Abstract: In the PNS, ciliary neurotrophic factor (CNTF) is found in significant amounts in the adult sciatic nerve, localized to myelin‐related Schwann cells (SCs). Levels are undetectable in newborn but high in adult animals. After crush injury, CNTF production is reduced, recovering only as the nerve regenerates; if regeneration does not occur, CNTF levels remain low. We have examined the coupling of CNTF expression to myelination in vitro and in vivo to determine if axon‐SC contact without myelination is sufficient to induce CNTF expression. Embryonic day 15 (E15) rat dorsal root ganglion (DRG) neuron and SCs were cocultured. CNTF was not detected in the DRGs either at E15 or after 2 days in vitro (div) by western blotting. However, after 10 div, CNTF could be identified and remained at constant levels up to 30 div. Depriving the cultures of ascorbic acid prevents myelination but not axon‐SC contact. This did not affect CNTF protein production. Using reverse transcriptase‐linked PCR (RT‐PCR) techniques, no CNTF message was present in DRG from day 14 or 15 fetal rats; but by 6 days in culture, message was detected in both myelinating and nonmyelinating cultures. Isolated SC cultures, without axonal contact, failed to express CNTF protein; however, mRNA was detected by RT‐PCR. Embryonic SC can be induced to synthesize CNTF in culture by axonal contact. Active myelination is not required.
Use of plasmid profile typing for surveillance of Salmonella enteritidis phage type 4 from humans, poultry and eggs

Summary Plasmids were found in 1022 of 1089 (94%) of drug–sensitive strains of Salmonella enteritidis phage type 4 from humans (sporadic and outbreak cases), poultry (chickens) and eggs in England and Wales in the 5-year period 1988–92 and 25 plasmid profile patterns were identified. Strains characterized by a single plasmid of 38 MDa predominated ( = plasmid profile type SE 38), comprising over 90% of isolates from humans, 70% from poultry and 92% from eggs. Eleven profile types were identified in strains from humans, 21 in strains from poultry and 3 in strains from eggs. Eight of the 11 patterns identified in human isolates were found in strains from poultry and 2 in strains from eggs. In contrast 15 patterns seen in poultry were not found in strains from humans. Four percent of strains from humans and 13% from poultry did not carry the 38 MDa plasmid but all strains from eggs were found to carry this plasmid. The second most common profile type in strains isolated between 1981 and 1988 was not identified in strains isolated from 1988–92. It is concluded that plasmid profile typing is a useful method for rapid differentiation within phage type 4 of S. enteritidis but that methods which can discriminate within the predominant profile type, SE 38, are now required.
La crisis económica internacional y el papel del G-20

La segunda reunion cumbre del G-20 en Londres arrojo resultados positivos, aunque todavia modestos, con vistas a resolver la crisis y dar lugar a un crecimiento sustentable a largo plazo. Los resultados concretos se relacionan con el compromiso de los miembros de contribuir a la recuperacion de la demanda global, mediante la capitalizacion de los organismos financieros multilaterales y la ejecucion de medidas fiscales y monetarias domesticas. En esta segunda reunion se adoptaron o comprometieron medidas efectivas y se acordo avanzar en la reforma de los organismos multilaterales de credito y la reestructuracion regulatoria y de supervision del sistema financiero internacional. La cuestion de mayor importancia es de caracter institucional, ya que se dispone otorgar al G-20 y al Consejo de Estabilidad Financiera, recientemente formado, la conduccion politica de gestion de la crisis y del diseno e instrumentacion de las reformas propuestas.
Evaluation of facial attractiveness for patients with malocclusion: a machine-learning technique employing Procrustes.

OBJECTIVE To establish an objective method for evaluating facial attractiveness from a set of orthodontic photographs. MATERIALS AND METHODS One hundred eight malocclusion patients randomly selected from six universities in China were randomly divided into nine groups, with each group containing an equal number of patients with Class I, II, and III malocclusions. Sixty-nine expert Chinese orthodontists ranked photographs of the patients (frontal, lateral, and frontal smiling photos) before and after orthodontic treatment from "most attractive" to "least attractive" in each group. A weighted mean ranking was then calculated for each patient, based on which a three-point scale was created. Procrustes superimposition was conducted on 101 landmarks identified on the photographs. A support vector regression (SVR) function was set up according to the coordinate values of identified landmarks of each photographic set and its corresponding grading. Its predictive ability was tested for each group in turn. RESULTS The average coincidence rate obtained for comparisons of the subjective ratings with the SVR evaluation was 71.8% according to 18 verification tests. CONCLUSIONS Geometric morphometrics combined with SVR may be a prospective method for objective comprehensive evaluation of facial attractiveness in the near future.
Rotterdam computed tomography score as a prognosticator in head-injured patients undergoing decompressive craniectomy.

BACKGROUND The Rotterdam computed tomography (CT) score was developed for prognostic purposes in traumatic brain injury (TBI). OBJECTIVE To examine the prognostic discrimination and prediction of the Rotterdam CT score in the case of patients undergoing decompressive craniectomy (DC) for TBI. METHODS The CT scans with the worst findings before DC were scored according to the Rotterdam CT classification. Mortality and Glasgow Outcome Scale score at the end of follow-up were used as outcome measures. Unfavorable and favorable outcomes were defined by a Glasgow Outcome Scale score of 1 to 3 and 4 to 5, respectively. We used binary logistic and proportional odds regression for prognostic analyses. RESULTS The relationship between the Rotterdam CT score and prognosis was quantified, and higher scores indicated worse patient outcomes. Univariate analysis showed that the Rotterdam CT score was significantly associated with mortality (odds ratio: 3.117, 95% confidence interval: 1.867-5.386; P < .001) and unfavorable outcomes (odds ratio: 2.612, 95% confidence interval: 1.733-3.939; P < .001). After adjustment for published outcome predictors of TBI in multivariate regression, the Rotterdam CT score remained an independent predictor of unfavorable outcomes (odds ratio: 1.830, 95% confidence interval: 1.043-3.212; P = .035). CONCLUSION For head-injured patients undergoing DC, the Rotterdam CT score provides great prognostic discrimination and is an independent predictor of unfavorable outcomes. We suggest that the Rotterdam CT score be included as a prognosticator in the overall assessment of clinical condition of TBI patients before DC.
The effect of type and concentration of vehicles on the dissolution rate of a poorly soluble drug (indomethacin) from liquisolid compacts.

PURPOSE For poorly soluble, highly permeable (Class II) drugs, such as indomethacin, the rate of oral absorption is often controlled by the dissolution rate in the gastrointestinal tract. Therefore together with the permeability, the solubility and dissolution behaviour of a drug are key determinants of its oral bioavailability. The object of the present study is to increase dissolution rate of indomethacin using liquisolid compacts. METHODS Several formulations of liquisolid compacts containing various ratios of drug: propylene glycol (ranging from 1:1 to 1:4) was prepared. In this study the ratio of microcrystalline cellulose (carrier) to silica (coating powder material) was 20 in all formulations. The dissolution behaviour of indomethacin from liquisolid compacts and conventional formulations was investigated at different pHs (1.2 and 7.2). RESULTS The results showed that liquisolid compacts demonstrated considerably higher drug dissolution rates than those of conventionally made capsules and directly compressed tablets containing indomethacin. This was due to increased wetting properties and surface of drug available for dissolution. Also it has been shown that the fraction of molecularly dispersed drug (FM) in the liquid medication of liquisolid systems was directly proportional to their indomethacin dissolution rates (DR). An attempt was made to correlate the percentage drug dissolved in 10-min with the solubility of indomethacin in different vehicles. A plot of the percentage drug dissolved against the solubility of indomethacin showed that the amount of drug dissolved increased linearly (correlation coefficient of 0.9994 and 0.996 at pH 7.2 and 1.2 respectively) with an increase in solubility of indomethacin in the vehicles. CONCLUSION The liquisolid compacts technique can be a promising alternative for the formulation of water insoluble drugs, such as indomethacin into rapid release tablets.