Miracle-dz/UniFAR-FTUs huggingface dataset:
Upregulation of miR‐362‐3p Modulates Proliferation and Anchorage‐Independent Growth by Directly Targeting Tob2 in Hepatocellular Carcinoma

The dysregulation of microRNAs (miRNAs) contributes to the pathogenesis of human malignancies, and miRNA expression can be affected by genetic and epigenetic changes, such as methylation of the CpG islands of their promoters. To identify miRNAs regulated by DNA methylation, the global miRNA expression profile was analyzed in two hepatocellular carcinoma (HCC) cell lines and two normal immortalized cell lines treated with 5‐Aza‐2′‐deoxycytidine (DAC, an inhibitor of DNA methylation) plus TSA (Trichostatin A, histone deacetylase inhibitor). Results revealed that these epigenetic drugs differentially affect miRNA expression that is dependent or independent of cell type, especially miR‐362‐3p. miR‐362‐3p expression increased while methylation of its promoter significantly decreased in human HCC cells and tissues compared with normal cells and adjacent noncancerous tissues. Ectopic expression of miR‐362‐3p increased proliferation and anchorage‐independent soft agar growth and its expression inhibition had opposing effects that were associated with regulation of its direct target—Tob2 in HCC cells. Inhibition of Tob2 recapitulated the effects of miR‐362‐3p overexpression, whereas enforced Tob2 expression reversed the promoting effects of miR‐362‐3p. Tob2 expression was reduced in human primary HCCs compared to adjacent noncancerous tissues. Our findings suggest that dysregulation of miR‐362‐3p and Tob2 may contribute to HCC malignancy. J. Cell. Biochem. 116: 1563–1573, 2015. © 2015 Wiley Periodicals, Inc.
Gain and kinetics of activation in the G-protein cascade of phototransduction.

The guanine nucleotide binding protein (G protein) cascade underlying phototransduction is one of the best understood of all signaling pathways. The diffusional interactions of the proteins underlying the cascade have been analyzed, both at a macroscopic level and also in terms of the stochastic nature of the molecular contacts. In response to a single activated rhodopsin (R*) formed as a result of a single photon hit, it can be shown that molecules of the G-protein transducin will be activated approximately linearly with time. This, in turn, will cause the number of activated molecules of the effector protein (the phosphodiesterase) also to increase linearly with time. These kinetics of protein activation provide an accurate description of the time course of the rising phase of the photoreceptor's electrical response over a wide range of flash intensities. Recent estimates indicate that at room temperature each R* triggers activation of the phosphodiesterase at a rate of 1000-2000 subunits.s-1. Now that a quantitative description of the activation steps in transduction has been obtained, perhaps the greatest challenge for the future is to provide a comprehensive description of the shutoff reactions, so that a complete account of the photoreceptor's response to light can be achieved.
Participant Observation

the two is like comparing a n abacus to a computer of advanced design. The differences are far more numerous and important than are the similarities, so that studying the one, however intensively, may tell you nothing-or very little-of what you would like to know about the other. To know there are similarities and connections is not the same as being able to decide what the similarities and connections are. Chapter five (Keen) is principally a n application of Jungian psychoanalytic theories to the emotions. It is of little interest to those for whom archetype and anima are not working concepts. Chapter six (Fell) is existential philosophy applied to science in general and to psychology in particular. T o one who is not a professional philosopher it seemed no more than a curiosity.
Hormonal factors affecting glucose 6-phosphatase activity. 2. Some effects of diet and of alloxan diabetes in the rat.

A rise in liver glucose 6-phosphatase activity in rats which are made alloxan-diabetic or which are fasted has been reported (Ashmore, Hastings & Nesbett, 1954; Weber & Cantero, 1954; Langdon & Weakley, 1955). Because these and other investigations of the effects ofhypophysectomy (Harper & Young, 1959) have shown that the food intake of the animals may greatly affect the glucose 6phosphatase concentration in the liver, some effects of alterations in the diet, both in quantity and in composition, on liver glucose 6-phosphatase have been investigated. A preliminary account of some of these observations has already been published (Harper, 1956).
An 8 b 100 MSample/s CMOS pipelined folding ADC

When applied to folding ADCs, pipelining relieves the wide bandwidth requirement of the folding amplifier. A pipelined folding ADC prototyped using a 0.5 /spl mu/m CMOS process exhibits a DNL of /spl plusmn/0.4 LSB and an INL of /spl plusmn/1.3 LSB at 100 MSample/s. The chip occupies 1.4 mm/spl times/1.2 mm in active area and consumes 165 mW at 5 V.
[Exercise preconditioning attenuates pressure overload-induced pathological cardiac hypertrophy: potential role of HSF1 and NF-κB p65 signaling].

OBJECTIVE To observe the effect of exercise preconditioning (EP) on pressure overload-induced pathological cardiac hypertrophy and explore related mechanisms. METHODS Ten-week-old male Sprague-Dawley rats (n = 80) were randomly divided into four groups via random number table method: sham, TAC, EP + sham and EP + TAC. Two EP groups were subjected to 4 weeks of treadmill training, and followed by sham and TAC operations. Eight weeks after the surgery, mean arterial pressure (MAP), cardiac morphology, mRNA expressions of the B-type natriuretic peptide (BNP) and heat shock protein (HSP) 70 and protein expression of the BNP, heat shock transcription factor 1 (HSF1), HSP70, nuclear factor κB (NF-κB) p65, and interleukin-2 (IL-2) were examined. RESULTS (1) Pathological cardiac hypertrophy index: eight weeks after TAC, MAP, heart size, HW/BW, cross-sectional area of the cardiomyocytes (CSA) and mRNA and protein expressions of BNP in the LV were all significantly higher in the TAC and EP + TAC groups than respective sham groups (all P < 0.05). HW/BW, CSA, and mRNA and protein expressions of BNP in the LV were significantly lower in EP + TAC group than in TAC group (all P < 0.05). (2) mRNA and protein expressions of HSF1 and HSP70 and nuclear HSF1 levels were significantly downregulated post TAC, however, EP treatment significantly increased the expression of HSF1 and nuclear HSF1 levels in TAC rats (all P < 0.05). (3) mRNA and protein expressions of NF-κB p65 and IL-2 were significantly increased in the TAC and EP + TAC groups compared with the respective sham groups (all P < 0.05), which were significantly downregulated in EP + TAC group compared to TAC group (all P < 0.05). CONCLUSIONS EP could effectively reduce the cardiac hypertrophic responses induced by TAC possibly through upregulating the expressions of HSF1 and HSP70 and inhibiting the expression of NF-κB p65 and its nuclear translocation.
A Genetic Programming Ensemble Method for Learning Dynamical System Models

Modelling complex dynamical systems plays a crucial role to understand several phenomena in different domains such as physics, engineering, biology and social sciences. In this paper, a genetic programming ensemble method is proposed to learn complex dynamical systems' underlying mathematical models, represented as differential equations, from systems' time series observations. The proposed method relies on decomposing the modelling space based on given variable dependencies. An ensemble of learners is then applied in this decomposed space and their output is combined to generate the final model. Two examples of complex dynamical systems are used to test the performance of the proposed methodology where the standard genetic programming method has struggled to find matching model equations. The empirical results show the effectiveness of the proposed methodology in learning closely matching structure of almost all system equations.
Media Development Models in Virtual World

This paper studies three digital media development models in virtual world. We characterize the revenue mechanisms behind each model, by incorporating the concept of horizontal and vertical supply chain integration of digital media industry. We incorporate the core value of digital media development in the first model, which considers digital media as information goods. Pricing, quality differentiation, quantity discrimination, and content design combined determine the VW popularity and the financial performance. Based on the first model, the second model incorporates the advertising revenue. It becomes essential to optimize both information goods revenue and advertising revenue, while increasing one would decrease the other due to the traffic dynamics. Based on the first two models, the third model considers both vertical and horizontal supply chain integration in the digital media industry. In this model, we add one extra dimension of network externality that leads to better understanding of consumers, suppliers, and their mutual interest. Based on these three models, we suggest several strategic implications that facilitate operations, increase revenue, and enhance consumer experience, including strategic alliance, reduction of virtual-real world barriers, and tailored advertisement.
İLKÖĞRETİM I. ve II. KADEMEDE BEDEN EĞİTİMİ DERSİNİN AMAÇLARINA GÖRE UYGULANMA DURUMUNUN İNCELENMESİ

Bu arastirmanin amaci, ilkogretim I. ve II. kademede beden egitimi dersinin; daha etkin ve yaygin duruma getirilmesinde, gerek programin cocuk gelisimine etkisinin yeniden yapilandirilmasi calismalarina katkida bulunabilmesi, gerekse dersin hedeflerine daha verimli ulasabilmesi icin beden egitimi dersinin islenisinde karsilasilan problemleri ve ogretmenlerin sinif ogretmeni veya beden egitimi ogretmeni olmalari acisindan beden egitimi dersinin amaclarina gore uygulanamama nedenleri incelenerek, sinif ogretmenleri ile beden egitimi ogretmenlerinin gorusleri arasindaki farklar cesitli istatistiksel islemler ile degerlendirilmistir. Tarama modelindeki bu calismanin evrenini, Ankara ilinde faaliyet gosteren, ilkogretim okullarinin I. ve II. kademesinde gorev yapan 12.605 beden egitimi ve sinif ogretmeni, orneklemini ise evrenden tesadufi olarak secilen 1576 beden egitimi ve sinif ogretmeni olusturmaktadir. Arastirma icin gerekli olan verilerin toplanmasinda arastirmanin amacina uygun olarak gelistirilen, kapsam ve gorunus gecerliligi icin, alanin uzmanlarinin gorus ve onerileri de dikkate alinarak hazirlanan bir anket kullanilmistir. Elde edilen verilerin cozumlenmesinde, SPSS 11.0 (Statistical Package for Social Sciences) istatistik paket programindan yararlanilmis, verilerin genel dagilim ozelliklerini belirlemek icin tanimlayici istatistik turlerinden frekans ve yuzde dagilimlari kullanilmis, olceklerdeki ifadelere iliskin goruslerin ortalama degerleri (  ) verilmis, ayrica parametrik istatistik tekniklerinden t-testi ve faktor analizi kullanilmistir. Verilerin istatistiksel analizi ve yorumlarda, p<0,05 anlamlilik duzeyi dikkate alinmistir. Arastirma sonucunda ilkogretim I. ve II. kademede; beden egitimi ogretmeni sayisinin yetersiz oldugu, sinif ogretmenlerinin lisans doneminden kaynaklanan egitim formasyonu eksikligi nedeniyle kendilerini yeterli gormedikleri, dersleri yanlis ve eksik isledikleri, ayrica cocuklarin ilkogretim birinci devreden ikinci devreye geciste motor beceri ve yetenek gelisimlerinin istenilen duzeyde saglanamadigi, beden egitimi derslerinin amaclarina ulasilabilmesi icin ogretmenlerin buyuk bir cogunlugu bu dersin brans ogretmenleri tarafindan yurutulmesi gerektigi hususunda gorus belirtmislerdir. Diger taraftan beden egitimi ders saatlerinin yetersiz olmasi, siniflarin kalabalik olmasi, beden egitimi dersi ile ilgili bilimsel arastirmalarin sonuclarinin ilkogretim okullari ve yetkililerce gozardi edilmesi ile okullardaki tesis, malzeme, arac-gerec eksikligi de beden egitimi dersi ogretim programlarinda amaclanan hedeflere ulasilmasini guclestirmektedir.
The effect of Punica granatum nanoparticles in phagocytic cells in patients with Multiple Myeloma

Multiple myeloma is a cancer that forms in a type of white blood cell called a plasma cell. Plasma cells help you fight infections by making antibodies that recognize and attack germs. This study aimed to investigate effect of aqueous extract and silver nanoparticles from Punica granatum peel on the ability of phagocytic cells in patients with Multiple myeloma. Chemical components of Punica granatum were investigated. It was found that they contained most of chemical compounds such as Glycosides, Flavonoids, Alkaloids, Saponins, Tanins, Resins, Terpenes, and Steroids. Our results showed the presence of a significant decrease (p˂0.001) in ability of phagocytic cells in patients, when the results were compared to the control group which included healthy individuals. While, the results showed increase in the percentage of phagocytic cells after added Punica granatum peel silver nanoparticles by increases activity of NADPH enzyme. This increase indicates that the Punica granatum peel silver nanoparticles contain chemical components which act as immune modulators by increase of the percentage of phagocytic cells to engulf bacteria.
La phosphorylation du récepteur mGlu₂ du glutamate : mécanisme clé de son cross talk fonctionnel avec le récepteur 5-HT2A de la sérotonine

Les recepteurs 5-HT2A de la serotonine et mGlu2 du glutamate suscitent un grand interet vu la deregulation des deux recepteurs observee dans la schizophrenie et leur statut de cibles des antipsychotiques dits atypiques et de nouvelle generation, respectivement. Meme si les antipsychotiques atypiques ciblant le recepteur 5-HT2A ont montre une efficacite contre les symptomes positifs, leur effet reste tres limite contre les symptomes negatifs et cognitifs, et leurs effets secondaires nombreux. Depuis les annees 1990, une nouvelle classe d’antipsychotiques ciblant le systeme glutamatergique, en particulier le recepteur mGlu2, est en developpement. Les tests cliniques n’ont montre leur efficacite que pour les patients n’ayant pas ete traites auparavant par des antipsychotiques atypiques. Ceci suggere une interaction fonctionnelle forte entre les recepteurs 5-HT2A et mGlu2 dans le mode d’action de ces deux classes d’antipsychotiques. De plus, plusieurs etudes ont demontre l’existence d’un heteromere des deux recepteurs dans le cortex prefrontal qui semble important pour la reponse aux hallucinogenes et aux antipsychotiques ciblant l’un ou l’autre recepteur. Ainsi, etant donne l’impact du profil de phosphorylation adopte par les recepteurs couples aux proteines G (RCPG) sur leur fonction, j’ai caracterise au cours de ma these l’impact de la co-expression du recepteur 5-HT2A sur le profil de phosphorylation du recepteur mGlu2 en reponse a differentes stimulations. Parmi les 5 sites de phosphorylation identifies, la phosphorylation de la Ser843 est potentialisee en reponse a la stimulation par un agoniste du recepteur mGlu2 uniquement lorsque le recepteur 5-HT2A est co-exprime. Ces resultats ont ete valides grâce a la generation d’un nouvel anticorps dirige specifiquement contre la forme phosphorylee de la Ser843 sur culture cellulaire HEK-293 et in vivo dans le cortex prefrontal de souris, region ou les deux recepteurs sont co-exprimes. Des etudes fonctionnelles ont demontre que la phosphorylation de la Ser843 est necessaire a la potentialisation de l’activite Gi/o du recepteur mGlu2 en reponse a ses agonistes et constitue un cross-talk fonctionnel entre les deux recepteurs puisque les agonistes du recepteur 5-HT2A stimulent egalement la phosphorylation de la Ser843 du recepteur mGlu2. Ainsi, mes resultats de these ont permis d’identifier la phosphorylation du recepteur mGlu2 sur la Ser843 comme un evenement moleculaire cle du cross-talk fonctionnel avec le recepteur 5-HT2A et apporte un element important dans la comprehension du mode d’action des antipsychotiques atypiques et de nouvelle generation.
Changes in Kinematic Coupling Among the Rearfoot, Midfoot, and Forefoot Segments During Running and Walking.

BACKGROUND Understanding the concept of kinematic coupling is essential when selecting the appropriate therapeutic strategy and grasping mechanisms for the occurrence of injuries. A previous study reported that kinematic coupling between the rearfoot and shank during running and walking were different. However, because foot mobility involves not only the rearfoot but also the midfoot or forefoot, kinematic coupling is likely to occur among the rearfoot, midfoot, and forefoot segments. We investigated changes in kinematic coupling among the rearfoot, midfoot, and forefoot segments during running and walking. METHODS Ten healthy young men were instructed to run (2.5 ms-1) and walk (1.3 ms-1) on a treadmill at speeds set by the examiner. The three-dimensional joint angles of the rearfoot, midfoot, and forefoot were calculated based on the Leardini foot model Kinematic coupling was evaluated with the absolute value of the cross-correlation coefficients and coupling angles obtained by using a vector coding technique. RESULTS The cross-correlation coefficient between rearfoot eversion/inversion and midfoot dorsiflexion/plantarflexion was significantly higher during running ( r = 0.79) than during walking ( r = 0.58), suggesting that running requires stronger kinematic coupling between rearfoot eversion/inversion and midfoot plantarflexion/dorsiflexion than walking. Furthermore, the coupling angle between midfoot eversion/inversion and forefoot eversion/inversion was significantly less during running (30.0°) than during walking (40.7°) ( P < .05). Hence, the magnitude of midfoot frontal plane excursion during running was greater than that during walking. CONCLUSIONS Excessive rearfoot eversion during running is likely to lead to excessive midfoot dorsiflexion, and such abnormal kinematic coupling between the rearfoot and midfoot may be associated with mechanisms for the occurrence of injuries.
Prise en charge des agénésies dentaires multiples chez l'enfant

Les agenesies multiples sont des maladies rares dont les etiologies sont variees. Elles sont dans la majorite des cas d’origine genetique : elles peuvent etre isolees ou bien faire partie d’un syndrome polymalformatif comme le plus souvent celui de la dysplasie ectodermique. Un diagnostic clinique complet devra etre realise, des le plus jeune âge du patient, afin de mettre en evidence les differentes consequences physiologiques, esthetiques et psychologiques. Il permettra d’etablir un plan de traitement adequat et evolutif durant la croissance. Les decisions therapeutiques necessitent une collaboration multidisciplinaire. Des centres de references et competences des maladies rares ont ete mis en place afin d’apporter une aide au diagnostic et a l’orientation de ces jeunes patients. La prise en charge est souvent longue et complexe. Les differentes therapeutiques conservatrices, orthodontiques, prothetiques et implantaires necessitent une mise en confiance de l’enfant et une implication de son entourage.
Immortalization of normal human embryonic fibroblasts by introduction of either the human papillomavirus type 16 E6 or E7 gene alone

The ability of the human papillomavirus type 16 (HPV‐16) E6 or E7 gene to induce immortalization of normal human embryonic fibroblast WHE‐7 cells was examined. WHE‐7 cells at 9 population doublings (PD) were infected with retrovirus vectors encoding either HPV‐16 E6 or E7 alone or both E6 and E7 (E6/E7). One of 4 isolated clones carrying E6 alone became immortal and is currently at >445 PD. Four of 4 isolated clones carrying E7 alone escaped from crisis and are currently at >330 PD. Three of 5 isolated clones carrying E6/E7 were also immortalized and are currently at >268 PD. The immortal clone carrying E6 only and 2 of the 3 immortal clones carrying E6/E7 expressed a high level of E6 protein, and all the immortal clones carrying E7 alone and the other immortal clone carrying E6/E7 expressed a high level of E7 protein when compared to their mortal or precrisis clones. The immortal clones expressing a high level of E6 or E7 protein were positive for telomerase activity or an alternative mechanism of telomere maintenance, respectively, known as ALT (alternative lengthening of telomeres). All the mortal or precrisis clones were negative for both phenotypes. All the immortal clones exhibited abrogation of G1 arrest after DNA damage by X‐ray irradiation. The expression of INK4a protein (p16INK4a) was undetectable in the E6‐infected mortal and immortal clones, whereas Rb protein (pRb) was hyperphosphorylated only in the immortal clone. The p16INK4a protein was overexpressed in all the E7‐infected immortal clones and their clones in the pre‐crisis period as well as all the E6/E7‐infected mortal and immortal clones, but the pRb expression was downregulated in all of these clones. These results demonstrate for the first time to our knowledge that HPV‐16 E6 or E7 alone can induce immortalization of normal human embryonic fibroblasts. Inactivation of p16INK4a/pRb pathways in combination with activation of a telomere maintenance mechanism is suggested to be necessary for immortalization of normal human embryonic fibroblasts by these viral oncogenes. The susceptibility of human cells to immortalization may be related to the state of differentiation of the cells. © 2003 Wiley‐Liss, Inc.
Cluster analysis in retail segmentation for credit scoring

The aim of this paper is to segment retail clients by using adaptive Mahalanobis clustering in a way that each segment can be suitable for separate credit scoring development such that a better risk assessment of retail clients could be accomplished. A real data set on retail clients from a Croatian bank was used in the paper. Grouping of the data point set is carried out by using the adaptive Mahalanobis partitioning algorithm (see, e.g., [20]). It is an incremental algorithm, which recognizes ellipsoidal clusters with the main axes in the directions of eigenvectors of the corresponding covariance matrix of the data set. On the basis of the given data set, by using the well-known DIRECT algorithm for global optimization it is possible to search successively for an optimal partition with k=2,3,. clusters. After that, a partition with the most appropriate number of clusters is determined by using various validity indexes. Based on the description of each cluster, banks could decide to develop a separate credit scoring model for each cluster as well as to create a business strategy customized to each cluster.
ATPG aspects of FSM verification

Algorithms are presented for finite state machine (FSM) verification and image computation which improve on the results of O. Coudert et al (1989), giving 1-4 orders of magnitude speedup. Novel features include primary input splitting-this PODEM feature enlarges the search space but shortens the search due to implications. Another new feature, identical subtree recombination, is shown to be effective for iterative networks (eg, serial multipliers). The free-variable recognition feature prevents unbalanced bipartitioning trees in tautological subspaces. Finally, reached set pruning is significant when the image contains large numbers of previously reached states. <<ETX>>
Immunization with Modified Vaccinia Virus Ankara-Based Recombinant Vaccine against Severe Acute Respiratory Syndrome Is Associated with Enhanced Hepatitis in Ferrets

ABSTRACT Severe acute respiratory syndrome (SARS) caused by a newly identified coronavirus (SARS-CoV) is a serious emerging human infectious disease. In this report, we immunized ferrets (Mustela putorius furo) with recombinant modified vaccinia virus Ankara (rMVA) expressing the SARS-CoV spike (S) protein. Immunized ferrets developed a more rapid and vigorous neutralizing antibody response than control animals after challenge with SARS-CoV; however, they also exhibited strong inflammatory responses in liver tissue. Inflammation in control animals exposed to SARS-CoV was relatively mild. Thus, our data suggest that vaccination with rMVA expressing SARS-CoV S protein is associated with enhanced hepatitis.