Miracle-dz/UniFAR-FTUs huggingface dataset:
Cutting Edge: The Direct Action of Type I IFN on CD4 T Cells Is Critical for Sustaining Clonal Expansion in Response to a Viral but Not a Bacterial Infection1

The action of type I IFN (IFN-I) on APCs is well studied, but their direct effect on CD4 T cells is unclear. To address this, we transferred IFN-I receptor-deficient (IFN-IR0) and -sufficient (wild-type, WT) TCR-transgenic CD4 T cells into WT mice and analyzed their response to immunization. In response to lymphocytic choriomeningitis virus immunization, WT CD4 T cells expanded ∼100-fold, whereas IFN-IR0 CD4 T cells expanded <10-fold. However, both WT and IFN-IR0 CD4 T cells expanded ∼10-fold after Listeria monocytogenes immunization. Poor expansion of IFN-IR0 CD4 T cells after lymphocytic choriomeningitis virus immunization was not due to a defect in proliferation or initial activation but to poor survival of the daughter cells. Thus, direct IFN-I signals can play either a critical or minimal role in CD4 T cell clonal expansion depending on the specific pathogen.
Metschnikowia Strains Isolated from Botrytized Grapes Antagonize Fungal and Bacterial Growth by Iron Depletion

ABSTRACT Noble-rotted grapes are colonized by complex microbial populations. I isolated pigment-producing Metschnikowia strains from noble-rotted grapes that had antagonistic activity against filamentous fungi, yeasts, and bacteria. A red-maroon pigment was formed from a diffusible colorless precursor released by the cells into the medium. The conversion of the precursor required iron and could occur both in the cells (red colonies) and in the medium (red halos around colonies). The intensity of pigmentation was correlated with the intensity of the antimicrobial activity. Mutants that did not form pigment also lacked antifungal activity. Within the pigmented halos, conidia of the sensitive fungi did not germinate, and their hyphae did not grow and frequently lysed at the tips. Supplementation of the medium with iron reduced the size of the halos and the inhibition zones, while it increased the pigment accumulation by the colonies. The iron-binding agent tropolone had a similar effect, so I hypothesize that pigmented Metschnikowia isolates inhibit the growth of the sensitive microorganisms by pigment formation, which depletes the free iron in the medium. As the pigment is a large nondiffusible complex produced in the presence of both low and high concentrations of ferric ions, the proposed mechanism is different from the mechanisms operating in microbes that release siderophores into the environment for iron acquisition.
Effect of Verapamil Enantiomers and Metabolites on Cardiac K+ Channels Expressed in Xenopus Oocytes

The effect of verapamil and its enantiomers and metabolites on cardiac action potential repolarizing potassium channels was tested. For this purpose, the potassium channels Kv1.1, Kv1.5, Kir2.1, and HERG, and the IsK subunit of the IKs-channel complex were expressed in Xenopus oocytes and two-electrode voltage-clamp experiments were performed. Verapamil induced a concentration-dependent block of Kv1.1-, Kv1.5-, IKs-, and HERG-induced currents with IC50 values of 14.0 ± 2.7 μM (n = 4), 5.1 ± 0.5 μM (n = 6), 161.0 ± 26.3 μM (n = 4), and 3.8 ± 0.2 μM (n = 5), respectively. The same potency of HERG channel inhibition was observed for the optical enantiomers (+)-verapamil (IC50 = 3.5 ± 0.4 μM, n = 5) and (–)-verapamil (IC50 = 4.0 ± 0.7 μM, n = 4), as well as the derivatives norverapamil (D591; IC50 = 3.8 ± 0.3 μM, n = 4) and D703 (IC50 = 2.2 ± 0.4 μM, n = 4). The verapamil metabolites D620 and D617 did not block HERG-induced currents at concentrations of up to 30 μM (n = 3). These results demonstrate that cardiac delayed rectifier potassium currents are sensitive targets to calcium channel blockers.
Role of a major autoepitope in forming the DNA binding site of the p70 (Ku) antigen

The Ku antigen is a heterodimer consisting of 70- and 80-kD protein subunits that binds to termini of double-stranded DNA. DNA binding appears to be mediated partly by the 70-kD (p70) subunit, but the precise mechanism of its association with DNA is unclear. High-titer autoantibodies in sera from certain patients with systemic lupus erythematosus recognize at least eight distinct epitopes of Ku, and inhibit DNA binding. In the present studies, the binding of DNA to truncated p70 fusion proteins was determined in Southwestern blots and DNA immunoprecipitation assays. Appropriate folding of the p70 protein was crucial for efficient DNA binding. The minimal DNA binding site, amino acids 536-609, contains a major conformational autoepitope of p70 (amino acids 560-609). Deletion of amino acids 601-609, or substitution of ala-ala-ala for lys-ser-gly at positions 591-593, eliminated DNA binding as well as autoantibody binding, suggesting that the same secondary or supersecondary structure is involved in both DNA binding and autoantibody recognition. Residues within the DNA binding site/autoepitope closely resemble the helix-turn-helix motif in bacteriophage lambda Cro protein and certain other DNA binding proteins, and mutations predicted to destabilize this structure eliminated DNA binding. Adjacent to the helix-turn-helix is a highly basic domain (positions 539-559) that was also required for DNA binding. The findings suggest that the DNA binding site of p70 consists of a basic domain adjacent to a helix-turn-helix structure that also forms a major autoepitope.
On the Frequency of Price Overreactions

This paper explores the frequency of price overreactions in the US stock market by focusing on the Dow Jones Industrial Index over the period 1990-2017. It uses two different methods (static and dynamic) to detect overreactions and then carries out various statistical tests (both parametric and non-parametric) including correlation analysis, augmented Dickey–Fuller tests (ADF), Granger causality tests, and regression analysis with dummy variables. The following hypotheses are tested: whether or not the frequency of overreactions varies over time (H1), is informative about crises (H2) and/or price movements (H3), and exhibits seasonality (H4). The null cannot be rejected except for H4, i.e. no seasonality is found. On the whole it appears that the frequency of overreactions can provide useful information about market developments and for designing trading strategies.
Does quercetin and vitamin C improve exercise performance, muscle damage, and body composition in male athletes?

Background: Quercetin is a bioflavonoid occurs in many food items. Some previous studies on quercetin showed the inconsistent results on exercise performance and muscle damage in athletes. The aim of this study was to determine the effects of 8 weeks of quercetin supplementation on exercise performance and muscle damage indices in student athletes. Methods: This placebo-controlled, double-blind clinical trial was conducted on 60 male students for 8 weeks. The subjects were randomly assigned to one of the four groups: a) quercetin (500 mg/day quercetin + 200 mg/day placebo), b) quercetin+ vitamin C (500 mg/day quercetin + 200 mg/day vitamin C), vitamin C (500 mg/day placebo + 200 mg/day vitamin C), and placebo (500 mg/day placebo + 200 mg/day placebo). Time to exhaustion (TTE) for measuring performance, aspartate transaminase (AST), and creatine kinase (CK) for measuring muscle damage and body fat percent (BFP) were measured before and after intervention. Results: CK levels reduced in group 1 significantly (P=0.045) and BFP reduced in group 1, 3, and 4, significantly, too (P=0.018, P=0.013, and P=0.043, respectively). Whereas statistically significant changes between groups were not observed for TTE, AST, CK, and BFP after 8 weeks of intervention. Conclusions: Supplementation with quercetin and vitamin C for 8 weeks did not improve exercise performance but reduced muscle damage and body fat percent in healthy subjects.
High resolution imaging of normal and osteoarthritic cartilage with optical coherence tomography.

OBJECTIVE We describe optical coherence tomography (OCT), a high resolution micron scale imaging technology, for assessment of osteoarthritic articular cartilage microstructure. OCT is analogous to ultrasound, measuring the intensity of backreflected infrared light rather than acoustical waves. METHODS OCT imaging was performed on over 100 sites on 20 normal and osteoarthritic cartilage specimens in vitro. RESULTS Microstructures that were identified included fibrillations, fibrosis, cartilage thickness, and new bone growth at resolutions between 5 and 15 microm. In addition, the polarization sensitivity of imaging suggested a diagnostic role of polarization spectroscopy. CONCLUSION OCT represents an attractive new technology for intraarticular imaging due to its high resolution (greater than any available clinical technology), ability to be integrated into small arthroscopes, compact portable design, and relatively low cost.
Estimation of Single Event Transient Voltage Pulses in VLSI Circuits From Heavy-Ion-Induced Transient Currents Measured in a Single MOSFET

Described is an estimation technique of single event transient voltage pulses in VLSI circuits from heavy-ion-induced transient currents measured in a single MOSFET. Waveforms of the transient voltage pulses are fully estimated by using transient current data obtained in various drain voltage conditions. Modeling of the irradiated device behavior for use in device/circuit simulations is not required. The concept is validated using a 2-D mixed-mode device simulation.
Diffusion-limited Reaction of Free Nitric Oxide with Erythrocytes*

Concentration changes of nitric oxide (NO) were monitored using an NO-sensitive electrode in phosphate-buffered saline (PBS) with either free oxyhemoglobin (oxyHb) or red blood cells (RBCs). In aerated PBS, the half-life of 0.9 μm NO is greater than 4 min. NO is undetectable (<50 nm) when added to a solution of oxyHb because the reaction of NO with oxyHb is rapid. The disappearance rate of NO in PBS containing RBCs is rapid, compared with PBS, but it is much slower (by a factor of approximately 650) than with an equivalent solution of free oxyHb. The half-life of NO is inversely proportional to the concentration of RBCs, independent of oxyHb concentration inside RBCs, and the disappearance rate of NO is first order in NO concentration and first order in the concentration of RBCs. After all the oxyHb reacts with NO to form methemoglobin, the disappearance rate of NO slows greatly. These data indicate that the reaction of NO with oxyhemoglobin within RBCs is limited by the diffusion of NO into the cell, which has also been shown previously for the reaction of O2 with deoxyhemoglobin. Experimental data show that the half-life of NO in the presence of 2.1 × 106 RBCs/ml is 4.2 s. From this value, we estimate that the half-life of NO in whole blood (5 × 109RBCs/ml) will be 1.8 ms. A simple analytical expression for the half-life of NO in PBS with RBCs was derived in this study based on a spherical diffusion model. The calculated half-life of NO from the expression is in good agreement with the experimental values.
Seasonal ITCZ migration dynamically controls the location of the (sub)tropical Atlantic biogeochemical divide

Significance Low concentrations of fixed nitrogen restrict phytoplankton growth in much of the low-latitude surface oceans. Diazotrophic cyanobacteria are capable of fixing atmospheric dinitrogen, thereby replenishing the overall pool of fixed nitrogen. As a consequence, spatial–temporal variability in diazotrophy can potentially influence the global nitrogen cycle. Here we show that movement in the region of elevated iron concentrations tied to the seasonal migration of the intertropical convergence zone drives a shift in the latitudinal distribution of dinitrogen fixation and corresponding phosphate depletion in surface waters. Surface nutrient concentrations and diazotrophic activity divide the (sub)tropical Atlantic into a high-phosphate, low-iron system in the south, and a low-phosphate, high-iron system in the north. Inorganic nitrogen depletion restricts productivity in much of the low-latitude oceans, generating a selective advantage for diazotrophic organisms capable of fixing atmospheric dinitrogen (N2). However, the abundance and activity of diazotrophs can in turn be controlled by the availability of other potentially limiting nutrients, including phosphorus (P) and iron (Fe). Here we present high-resolution data (∼0.3°) for dissolved iron, aluminum, and inorganic phosphorus that confirm the existence of a sharp north–south biogeochemical boundary in the surface nutrient concentrations of the (sub)tropical Atlantic Ocean. Combining satellite-based precipitation data with results from a previous study, we here demonstrate that wet deposition in the region of the intertropical convergence zone acts as the major dissolved iron source to surface waters. Moreover, corresponding observations of N2 fixation and the distribution of diazotrophic Trichodesmium spp. indicate that movement in the region of elevated dissolved iron as a result of the seasonal migration of the intertropical convergence zone drives a shift in the latitudinal distribution of diazotrophy and corresponding dissolved inorganic phosphorus depletion. These conclusions are consistent with the results of an idealized numerical model of the system. The boundary between the distinct biogeochemical systems of the (sub)tropical Atlantic thus appears to be defined by the diazotrophic response to spatial–temporal variability in external Fe inputs. Consequently, in addition to demonstrating a unique seasonal cycle forced by atmospheric nutrient inputs, we suggest that the underlying biogeochemical mechanisms would likely characterize the response of oligotrophic systems to altered environmental forcing over longer timescales.
Focus FMCW SAR Data Using the Wavenumber Domain Algorithm

The combination of frequency-modulation continuous-wave (FMCW) technology and synthetic aperture radar (SAR) promises a lightweight, cost-effective, and high-quality imaging sensor for remote sensing. However, the long signal duration time leads to the failure of the conventional start/stop approximation of the pulsed SAR. In this paper, a signal model is proposed to address the effects of the continuous motion during the transmit time on the echoed signal. Based on the model, an analytical point target reference spectrum is derived. From the spectrum, it will be seen that the continuous motion introduces an additional range-azimuth coupling term and a range walk term compared with the conventional pulsed SAR. The range walk term is well known, whereas the foregoing range-azimuth coupling term is formulated for the first time in the FMCW SAR community. For the squint and spotlight modes, these range walk and range-azimuth coupling terms might significantly degrade the image quality. In this paper, based on the proposed analytical signal model, we further discuss the application of the wavenumber domain algorithm for the FMCW SAR data. In addition, different approximations of the Stolt mapping are made to highlight the effect of the range-dependent higher-order range-azimuth coupling terms on the 2-D impulse responses. Finally, X-band simulated experiments and Ka-band real FMCW SAR data are used to validate the signal model and the processing method.
Unsecured Credit Supply, Credit Cycles, and Regulation

This paper explores the dynamics of unsecured credit supply over the recent credit cycle and around the passage of the CARD Act. We examine a unique data set of over 200,000 credit card mail solicitations to a representative sample of households and introduce credit card offers as a direct, informative measure of supply of such credit. Contrasting personal credit card offer dynamics before and after the passage of the CARD Act with those of personal loans, auto loans, and corporate credit cards, we find that lenders reduced credit supply of personal credit cards to nonprime borrowers in response to the CARD Act. Our analysis highlights the importance of separately examining supply and demand responses to assess the unintended consequences of regulation. Received January 30, 2016; editorial decision August 9, 2017 by Editor Philip Strahan.
Heterogeneity between women who received prenatal care in the third trimester and those who received no prenatal care.

OBJECTIVE To determine whether women who received prenatal care in the third trimester differed from those who received no prenatal care. METHODS We analyzed US birth certificates from 1990 through 1992, computing the distribution of live births for women who received prenatal care in the third trimester and for those who received no prenatal care according to eight demographic and pregnancy-related characteristics (age, race, marital status, residence, country of birth, education, interbirth interval, and parity). We used the Cochran-Mantel-Haenszel statistic to test the significance of the differences between the distributions for each characteristic, adjusting simultaneously for the other seven characteristics. RESULTS Women who received no prenatal care differed from women who received prenatal care in the third trimester for each of the demographic and pregnancy-related characteristics we examined. Among black and unmarried women, the two categories of prenatal care differed by more than 10%. CONCLUSIONS The characteristics of women who received no prenatal care and those of women who received prenatal care in the third trimester were heterogeneous. Strategies to promote earlier prenatal care should be specific and sensitive to women at risk for each category of late entry to prenatal care.
Range resolution improvement of airborne SAR images

This letter proposes an algorithm to improve the range resolution in airborne synthetic aperture radar (SAR) data by coherently combining an interferometric image pair, i.e., two images acquired with slightly different viewing angles. This algorithm is based on the wavenumber shift principle. In contrast to other methods, developed for application to spaceborne SAR data, the proposed algorithm takes the nonlinear effects due the strong variations in incidence angle in airborne SAR data into account. The proposed method is applied to SAR data of German Aerospace Center (DLR)'s E-SAR sensor. Quantitative verification results are obtained by measuring the resolution of several corner reflectors placed in the area under study, as well as the resolution of speckle of different areas. It is demonstrated that a resolution improvement of almost a factor of two can be achieved by incorporating a second interferometric image, which can be acquired easily with an airborne sensor.
Density-dependent dispersal and spatial population dynamics

The synchronization of the dynamics of spatially subdivided populations is of both fundamental and applied interest in population biology. Based on theoretical studies, dispersal movements have been inferred to be one of the most general causes of population synchrony, yet no empirical study has mapped distance-dependent estimates of movement rates on the actual pattern of synchrony in species that are known to exhibit population synchrony. Northern vole and lemming species are particularly well-known for their spatially synchronized population dynamics. Here, we use results from an experimental study to demonstrate that tundra vole dispersal movements did not act to synchronize population dynamics in fragmented habitats. In contrast to the constant dispersal rate assumed in earlier theoretical studies, the tundra vole, and many other species, exhibit negative density-dependent dispersal. Simulations of a simple mathematical model, parametrized on the basis of our experimental data, verify the empirical results, namely that the observed negative density-dependent dispersal did not have a significant synchronizing effect.
A Comment on Servaas Storm’s “The New Normal”

Abstract Accepting Professor Storm’s demolition of total factor productivity growth and production function-based reasoning, this article argues that to understand the great financial crisis and its aftermath requires an evolutionary perspective that goes beyond hysteresis and secular stagnation, that escapes the reification of potential output, and that recognizes how structural forces and not merely deficient demand have affected actual economic growth. It also requires a commonsense understanding of actual American social conditions—no, the middle class has not vanished—and an appreciation that raising living standards under present conditions requires social balance—a new emphasis on collective economic security and public consumption goods.
The impact of adding weight-bearing exercise versus nonweight bearing programs to the medical treatment of elderly patients with osteoporosis

Background: Osteoporosis is a major public health problem affecting the elderly population, particularly women. The objective of the study was to evaluate the effects of adding weight-bearing exercise as opposed to nonweight-bearing programs to the medical treatment of bone mineral density (BMD) and health-related quality of life (HRQoL) of elderly patients with osteoporosis. Materials and Methods: Participating in the study were 40 elderly osteoporotic patients (27 females and 13 males), with ages ranging from 60 to 67 years, who were receiving medical treatment for osteoporosis. They were assigned randomly into two groups: Group-I: Twenty patients practiced weight-bearing exercises. Group-II: Twenty patients did nonweight-bearing exercises. All patients trained for 45-60 min/session, two sessions/week for 6 months. BMD of the lumbar spine, right neck of femur, and right distal radial head of all patients were measured by dual-energy X-ray absorptiometry before and after both treatment programs. In addition, the QoL was measured by means of the HRQoL “ECOS-16” questionnaire. Results: T-tests proved that mean values of BMD of the lumbar spine, right neck of femur and right distal radial head were significantly increased in both groups with greater improvement in the weight-bearing group. The QoL was significantly improved in both groups, but the difference between them was not significant. Conclusion: Addition of weight-bearing exercise program to medical treatment increases BMD more than nonweight-bearing exercise in elderly subjects with osteoporosis. Furthermore, both weight-bearing and nonweight-bearing exercise programs significantly improved the QoL of patients with osteoporosis.
Albuminuria among Alaska Natives – Findings from the Genetics of Coronary Artery Disease in Alaska Natives (GOCADAN) Study

Background: The prevalence and associated risk factors for albuminuria and low-grade albuminuria in Alaska Natives is not known. Methods: Cross-sectional analysis of the Genetics of Coronary Artery Disease in Alaska Natives Study. We included 1,026 individuals, who represent 85% of the study participants for whom complete data were available. Risk factors examined were age, sex, education, diabetes, hypertension, obesity, lipids, C-reactive protein, angiotensin-converting enzyme inhibitor use, and smoking status. Urine albumin excretion was estimated by the albumin/creatinine ratio measured from a single random morning urine sample. Albuminuria was defined as an albumin/creatinine ratio of ≧30 mg/g. Low-grade albuminuria was defined as an albumin/creatinine ratio of 10 to <30 mg/g. Results: The mean age was 42 years and over half were female. Diabetes prevalence was low at 3% and the prevalence of hypertension was 20%. The prevalence of albuminuria was 6%; the prevalence of low-grade albuminuria was 12%. Individuals with diabetes or hypertension were 3 times more likely to have albuminuria than those without these conditions [odd ratios: diabetes 3.0 (1.2–7.9) and hypertension 3.0 (1.2–7.3)]. Conclusions: The burden of albuminuria is low. Comprehensive programs and policies are important given the rise in diabetes and hypertension among Alaska Natives.