Miracle-dz/UniFAR-FTUs huggingface dataset:
Temperature preconditioning of isolated rat hearts – a potent cardioprotective mechanism involving a reduction in oxidative stress and inhibition of the mitochondrial permeability transition pore

We investigate whether temperature preconditioning (TP), induced by short‐term hypothermic perfusion and rewarming, may protect hearts against ischaemic/reperfusion injury like ischaemic preconditioning (IP). Isolated rat hearts were perfused for 40 min, followed by 25 min global ischaemia and 60 min reperfusion (37°C). During pre‐ischaemia, IP hearts underwent three cycles of 2 min global ischaemia and 3 min reperfusion at 37°C, whereas TP hearts received three cycles of 2 min hypothermic perfusion (26°C) interspersed by 3 min normothermic perfusion. Other hearts received a single 6 min hypothermic perfusion (SHP) before ischaemia. Both IP and TP protocols increased levels of high energy phosphates in the pre‐ischaemic heart. During reperfusion, TP improved haemodynamic recovery, decreased arrhythmias and reduced necrotic damage (lactate dehydrogenase release) more than IP or SHP. Measurements of tissue NAD+ levels and calcium‐induced swelling of mitochondria isolated at 3 min reperfusion were consistent with greater inhibition of the mitochondrial permeability transition at reperfusion by TP than IP; this correlated with decreased protein carbonylation, a surrogate marker for oxidative stress. TP increased protein kinase Cɛ (PKCɛ) translocation to the particulate fraction and pretreatment with chelerythrine (PKC inhibitor) blocked the protective effect of TP. TP also increased phosphorylation of AMP‐activated protein kinase (AMPK) after 5 min index ischaemia, but not before ischaemia. Compound C (AMPK inhibitor) partially blocked cardioprotection by TP, suggesting that both PKC and AMPK may mediate the effects of TP. The presence of N‐(2‐mercaptopropionyl) glycine during TP also abolished cardioprotection, indicating an involvement of free radicals in the signalling mechanism.
Voltage‐gated ionic currents in an identified modulatory cell type controlling molluscan feeding

An important modulatory cell type, found in all molluscan feeding networks, was investigated using two‐electrode voltage‐ and current‐clamp methods. In the cerebral giant cells of Lymnaea, a transient inward Na+ current was identified with activation at −58 ± 2 mV. It was sensitive to tetrodotoxin only in high concentrations (≈ 50% block at 100 µm), a characteristic of Na+ channels in many molluscan neurons. A much smaller low‐threshold persistent Na+ current (activation at < −90 mV) was also identified. Two purely voltage‐sensitive outward K+ currents were also found: (i) a transient A‐current type which was activated at −59 ± 4 mV and blocked by 4‐aminopyridine; (ii) a sustained tetraethylammonium‐sensitive delayed rectifier current which was activated at −47 ± 2 mV. There was also evidence that a third, Ca2+‐activated, K+ channel made a contribution to the total outward current. No inwardly rectifying currents were found. Two Ca2+ currents were characterized: (i) a transient low‐voltage (−65 ± 2 mV) activated T‐type current, which was blocked in NiCl2 (2 mm) and was completely inactivated at ≈ −50 mV; (ii) A sustained high voltage (−40 ± 1 mV) activated current, which was blocked in CdCl2 (100 µm) but not in ω‐conotoxin GVIA (10 µm), ω‐agatoxin IVA (500 nm) or nifedipine (10 µm). This current was enhanced in Ba2+ saline. Current‐clamp experiments revealed how these different current types could define the membrane potential and firing properties of the cerebral giant cells, which are important in shaping the wide‐acting modulatory influence of this neuron on the rest of the feeding network.
Universal newborn hearing screening follow-up: a university clinic perspective.

PURPOSE To evaluate the referral and follow-up procedures at a university clinic to determine whether the early intervention program is achieving the goals of diagnosis of hearing loss by 3 months, amplification within 1 month of diagnosis, and intervention services by 6 months, as outlined in the Joint Committee on Infant Hearing (JCIH; 2007) position statement. METHOD Files for 142 infants were examined, and the following data were collected from each file: date of birth, birth hospital, hometown, parents' ages, ethnicity, nursery status (well baby or neonatal intensive care unit), medical history, age at initial evaluation and at diagnosis, results of evaluation(s), and age at hearing aid fitting and start of early intervention services. RESULTS Results revealed that 17% of infants were older than 3 months at the initial evaluation, and 18% of infants who needed further evaluation were lost to follow-up. None of the infants identified with hearing loss received amplification within 1 month of diagnosis or early intervention services by the age of 6 months. CONCLUSIONS The findings provide further evidence of the challenges of early intervention programs as stated by the JCIH (2007), and they emphasize the importance of communication between practitioners and implementation of monitoring systems and checks and balances to improve the efficacy of early intervention programs.
Palliative care services for ethnic groups in Leicester.

The objectives of this study were to examine current palliative care service provision and its use by the black and minority ethnic (BME) groups in Leicester. * The study comprised two stages, an initial pilot study using semistructured tape recorded interviews, followed by a structured interview schedule. It was set in the voluntary sector, a local hospice and one trust hospital in Leicester. The subjects were 33 palliative care patients and carers from BME groups. The findings identify some deficiencies in access to and provision of palliative care services to BME communities in the city of Leicester. There appears to be a low level of satisfaction among the sample. There are also differences within the group, with 50% indicating that things are unsatisfactory. The main area of need identified was information about illness and available services. The study concluded that providers of palliative care need to examine their policy and practices with regards to whether they are acceptable, accessible and culturally sensitive to the needs of BME groups. A low uptake of services is more likely to suggest inappropriate provision rather than lack of need. An extended version of this article appears in Richardson and Wilson Barnett (1995). *The term black and minority ethnic (BME) group has been used to refer to people from racial or other minorities who may be disadvantaged because of their racial bakground. There is no single accepted term, nor one that can be seen as politically correct or incorrect. It should, however, be recognised that there may be some people who do not identify themselves as black or a minority but who share a common experience of racism (Anthias, 1992; Cole, 1993).
Occupational asthma and occupational rhinitis in hairdressers.

BACKGROUND Hairdressers are at risk for occupational respiratory diseases, but the risk factors, causal agents, and underlying mechanisms are not completely defined. AIM To describe the features of a large group of hairdressers consecutively referred to our center for suspected occupational asthma (OA) over an 8-year period, the type of occupational respiratory diseases, the etiologic agents, and the diagnostic tests. RESULTS Forty-seven hairdressers (mean age, 25 years; range, 17 to 52 years) were studied. On the basis of the response to the specific inhalation challenge (SIC), 24 patients received a diagnosis of OA (51.1%), which was due to persulfate salts in 21 patients (87.5%), permanent hair dyes in 2 patients (8.3%), and latex in 1 patient (4.2%). Thirteen of these 24 patients (54.2%) also received a diagnosis of occupational rhinitis, which was due to persulfate salts in 11 patients (84.6%) and to paraphenylenediamine in two patients (15.4%). Patients with persulfate asthma had a long period of exposure to bleaching agents, a long latent period between the start of exposure and the onset of symptoms, and a prevalent eosinophilic airway inflammation in induced sputum. The skin-prick test with ammonium persulfate performed in a subset of patients gave negative results CONCLUSIONS In the present study, we confirmed that persulfate salts are the major agents involved in OA and occupational rhinitis in hairdressers. The positive response to the SIC in only a part of the population of symptomatic exposed workers, the period between the starting of exposure and the onset of symptoms, the type of response to the SIC, and the high frequency of association of asthma with other diseases such as dermatitis and rhinitis suggest an immunologic mechanism that remains to be elucidated.
DNA sequence variation and phylogenetic relationships among strains of Pseudomonas syringae pv. syringae inferred from restriction site maps and restriction fragment length polymorphism

We evaluated the restriction fragment length polymorphism of genomic DNA among 53 strains of the phytopathogenic bacterium Pseudomonas syringae pv. syringae. Twenty-nine strains were isolated from beans, and the rest were isolated from 11 other hosts. Southern blots of DNA digested with EcoRI or HindIII were hybridized to two random probes from a cosmid library of P. syringae pv. syringae and a hrp (hypersensitive reaction and pathogenicity) cluster cloned from P. syringae pv. syringae. The size of hybridizing fragments was determined, and a similarity matrix was constructed by comparing strains on a pairwise basis for the presence or absence of fragments. The proportion of shared fragments was then used to estimate sequence divergence. Dendrograms were produced by using the unweighted pair group method with averages and the neighbor-joining method. For the hrp region, BamHI, EcoRI, EcoRV, and HindIII restriction sites were mapped for six representative bean strains and used to construct EcoRI and HindIII restriction maps for all 30 strains pathogenic on beans. Restriction mapping revealed the presence of a 3-kb insertion in nine bean strains and a probable second insertion or deletion event on the left-hand side of the hrp cluster that biased estimates of nucleotide sequence divergence from fragment comparisons. This demonstrated that the determination of phylogenetic relationships among bacteria by using restriction fragment length polymorphism data requires mapping restriction sites to remove the effect of insertion or deletion events on the analysis. (ABSTRACT TRUNCATED AT 250 WORDS)
Sensorless control of a synchronous reluctance motor by finite elements model results

This paper concerns the development of a sensorless controller for synchronous reluctance motors based on finite elements model results. A hybrid solution is used, which includes an adaptive observer for not-zero speed operation and signal injection for standstill. The observer detects the flux linkages components in the two-phase stationary reference frame by the voltage model integration and closed loop correction feedback. This last is provided by a non-linear model of the rotor-fixed frame flux components achieved by finite elements computations. The rotor speed and position are identified by a phase-locked-loop algorithm. To improve the observer performance, the mechanical model is considered, which accounts for the finite elements mapping of the motor torque. The study is applied to a prototype of synchronous reluctance motor with flux barriers rotor, designed to have the same stator core of a commercial three-phase 3kW induction motor. Both realistic simulations (accounting for the non-linear machine behavior) and experimental test are presented, showing the performance of the proposed solution.
Effects of Trauma and Religiosity on Self-Esteem

Self-esteem is often lower among persons who have experienced trauma, but religiosity may ameliorate these psychological effects. The purpose of this paper was to examine the relationships among religiosity, self-esteem, and childhood exposure to trauma, utilizing data from the National Comorbidity Survey, a large (N = 8,098) nationally representative population survey in the 48 contiguous states of the USA that assessed religious practices, self-esteem, and exposure to trauma. Exposure to trauma in childhood was assessed through self-report of presence or absence of childhood physical abuse, sexual abuse, or neglect. Religiosity was assessed as the sum of responses to 4 self-report items (religious service attendance, use of religion for comfort and guidance, and importance of religion). Self-esteem was assessed on 9 self-report items adapted from the Rosenberg Self-Esteem Scale. Analysis of variance compared scores for persons who reported exposure to childhood abuse and differed in the value they placed on various religious practices on self-esteem. Persons who reported physical abuse, sexual abuse, or neglect in childhood had significantly lower mean self-esteem than those who did not report these events. There was also a main effect for religiosity in a comparison of persons who reported childhood sexual abuse with those who reported none. The High Religiosity group had higher mean self-esteem than the Medium and Low Religiosity groups. There was a significant interaction as those who reported childhood sexual abuse had lower mean self-esteem than peers who reported none in the Low and Medium Religiosity groups. Mean self-esteem for those who reported childhood sexual abuse was comparable to that of those who reported none in the High Religiosity group.
Knowledge-Driven Multi-Locus Analysis Reveals Gene-Gene Interactions Influencing HDL Cholesterol Level in Two Independent EMR-Linked Biobanks

Genome-wide association studies (GWAS) are routinely being used to examine the genetic contribution to complex human traits, such as high-density lipoprotein cholesterol (HDL-C). Although HDL-C levels are highly heritable (h2∼0.7), the genetic determinants identified through GWAS contribute to a small fraction of the variance in this trait. Reasons for this discrepancy may include rare variants, structural variants, gene-environment (GxE) interactions, and gene-gene (GxG) interactions. Clinical practice-based biobanks now allow investigators to address these challenges by conducting GWAS in the context of comprehensive electronic medical records (EMRs). Here we apply an EMR-based phenotyping approach, within the context of routine care, to replicate several known associations between HDL-C and previously characterized genetic variants: CETP (rs3764261, p = 1.22e-25), LIPC (rs11855284, p = 3.92e-14), LPL (rs12678919, p = 1.99e-7), and the APOA1/C3/A4/A5 locus (rs964184, p = 1.06e-5), all adjusted for age, gender, body mass index (BMI), and smoking status. By using a novel approach which censors data based on relevant co-morbidities and lipid modifying medications to construct a more rigorous HDL-C phenotype, we identified an association between HDL-C and TRIB1, a gene which previously resisted identification in studies with larger sample sizes. Through the application of additional analytical strategies incorporating biological knowledge, we further identified 11 significant GxG interaction models in our discovery cohort, 8 of which show evidence of replication in a second biobank cohort. The strongest predictive model included a pairwise interaction between LPL (which modulates the incorporation of triglyceride into HDL) and ABCA1 (which modulates the incorporation of free cholesterol into HDL). These results demonstrate that gene-gene interactions modulate complex human traits, including HDL cholesterol.
Macrophage permissiveness for Legionella pneumophila growth modulated by iron

We have investigated the modulation of iron in two populations of macrophages which differ in susceptibility to Legionella pneumophila intracellular proliferation. Previously, we reported that thioglycolate-elicited peritoneal macrophages obtained from the inbred A/J mouse strain readily support the intracellular growth of L. pneumophila, while resident macrophages from the same strain do not. In this study, we show that A/J elicited macrophages exhibit markedly higher expression of transferrin receptor and intracellular iron content than A/J resident macrophages. Furthermore, apotransferrin and desferrioxamine inhibited the intracellular proliferation of L. pneumophila in elicited macrophages, and this suppression was reversed by the additions of Fe-transferrin or ferric nitrilotriacetate. Fe-transferrin and ferric nitrilotriacetate did not further increase the intracellular proliferation of L. pneumophila in thioglycolate-elicited macrophages. However, ferric citrate and ferric nitrilotriacetate stimulated in a dose-dependent manner the growth of L. pneumophila in resident macrophages. Furthermore, equimolar concentrations of desferrioxamine reversed the stimulatory effect of iron in these resident cells. These data provide evidence supporting the hypothesis that differences in susceptibility to L. pneumophila growth between permissive elicited macrophages and nonpermissive resident macrophages from the A/J mouse strain are due to intracellular availability of iron.
ECP technique based capacitor-less LDO with high PSRR at low frequencies, −89dB PSRR at 1MHz and enhanced transient response

An external capacitor-less low dropout (LDO) voltage regulator with high PSRR and enhanced transient response is presented. The novel idea is applying a replica circuit which can pull excessive current. Excessive Current Pulling (ECP) technique decreases the equivalent output impedance to enhance transient response. The proposed LDO has been simulated in 0.18 μm CMOS Technology. Its regulated output voltage is 1.6 V with the power supply of 1.8 V. The proposed technique has the advantages of simulated PSRR of −88.7 dB at 1 MHz and −67 dB at 100 KHz. Overshoots and undershoots are less than 45.2 mV and 30 mV under the load varies from 50 mA to 0 mA with rise/fall time of 100ns, respectively. Simulation results show that the achieved line regulation is 28.3 mV/V and the load regulation is 0.33 %/mA.
Mechanisms of iron regulation in mycobacteria: role in physiology and virulence

The role of iron in mycobacteria as in other bacteria goes beyond the need for this essential cofactor. Limitation of this metal triggers an extensive response aimed at increasing iron acquisition while coping with iron deficiency. In contrast, iron‐rich environments prompt these prokaryotes to induce synthesis of iron storage molecules and to increase mechanisms of protection against iron‐mediated oxidative damage. The response to changes in iron availability is strictly regulated in order to maintain sufficient but not excessive and potentially toxic levels of iron in the cell. This response is also linked to other important processes such as protection against oxidative stress and virulence. In bacteria, iron metabolism is regulated by controlling transcription of genes involved in iron uptake, transport and storage. In mycobacteria, this role is fulfilled by the iron‐dependent regulator IdeR. IdeR is an essential protein in Mycobacterium tuberculosis, the causative agent of human tuberculosis. It functions as a repressor of iron acquisition genes, but is also an activator of iron storage genes and a positive regulator of oxidative stress responses.
The productivity performance of enterprise IT investment and the moderating effect of staff - based on the evidence of manufacturing listed companies

Aiming at the problem of the effect of information technology and its impact on the performance of the manufacturing industry. Based on the existing research, we sets information hardware and software investment as explanatory variables, the number of highly educated employees as the moderator variables, the enterprise performance as the explanatory variables. This paper makes an empirical study of listed companies in manufacturing industry, the result shows that informationization hardware investment has a positive impact on enterprise performance, informationization software investment has a negative effect on enterprise performance. Besides, high degree employees will play a positive role in moderating the relationship between informatization hardware capital and enterprise performance, promote the positive effect on performance. On the contrary, the high degree employees plays a negative moderating role in the relationship between capital and enterprise information software performance, reduce the negative impact on the company's performance, so the highly educated staff affects the enterprise performance from the two path, and eventually promote to improve the performance of the company.
Effect of rifampicin-based antitubercular therapy on nevirapine plasma concentrations in South African adults with HIV-associated tuberculosis.

BACKGROUND AND OBJECTIVES Nevirapine-containing antiretroviral therapy (ART) and rifampicin-based antitubercular therapy are commonly co-administered in Africa, where nevirapine is often the only available non-nucleoside reverse transcriptase inhibitor. Rifampicin induces the metabolism of nevirapine, but the extent of the reduction in nevirapine concentrations has varied widely in previous studies. We describe the steady-state pharmacokinetics of nevirapine during and after antitubercular therapy in South African patients. METHODS Sixteen patients receiving ART including standard doses of nevirapine were admitted twice for intensive pharmacokinetic sampling: during and after rifampicin-based antitubercular therapy. RESULTS Geometric mean ratios for nevirapine pharmacokinetic parameters during versus after antitubercular therapy were 0.61 [90% confidence interval (CI) 0.49-0.79] for Cmax, 0.64 (90% CI 0.52-0.80) for area under the curve up to 12 h (AUC(0-12)) and 0.68 (90% CI 0.53-0.86) for Cmin. Nevirapine Cmin was subtherapeutic (<3 mg/L) in six patients during antitubercular therapy (one of whom developed virological failure) and in none afterwards. There was no correlation between rifampicin concentrations and the degree of nevirapine induction assessed by the proportional change in nevirapine concentrations between the two admissions. The ratio of nevirapine AUC(0-12) to the AUC(0-12) of its 12-hydroxy metabolite was significantly lower in the presence of antitubercular therapy, consistent with induced metabolism. CONCLUSIONS Nevirapine concentrations were significantly decreased by concomitant rifampicin-based antitubercular therapy and a high proportion of patients had subtherapeutic plasma concentrations. Further study in African patients is required to determine the implications for treatment outcomes.
Smiling faces and other rewards: using the Behavioral Assessment and Research System (BARS) with unique populations.

Concern about the exposure of children and adolescents to occupational and environmental hazards has increased, and so has the need to develop testing methods that can adequately assess the effects of exposure in children. A computerized testing system, the Behavioral Assessment and Research System (BARS), was successfully modified to test both younger populations and populations which do not speak English, the original language of the battery. These adaptations were modifications of the existing features of the BARS system which was designed to assess the broadest possible audience: simple language instructions broken down into basic concepts (step-by-step training with competency testing at each instruction step); a token dispenser along with a "smiling face" stimulus that reinforced appropriate performance; and adjustable parameter settings (e.g., number of trials, difficulty). Data from four groups demonstrate the feasibility of using BARS with children as young as preschool age and for non-English speaking children.