Miracle-dz/UniFAR-FTUs huggingface dataset:
The rat pineal gland comprises an endocannabinoid system

Abstract: In the mammalian pineal gland, the rhythm in melatonin biosynthesis depends on the norepinephrine (NE)‐driven regulation of arylalkylamine N‐acetyltransferase (AANAT), the penultimate enzyme of melatonin biosynthesis. A recent study showed that phytocannabinoids like tetrahydrocannabinol reduce AANAT activity and attenuate NE‐induced melatonin biosynthesis in rat pineal glands, raising the possibility that an endocannabinoid system is present in the pineal gland. To test this hypothesis, we analyzed cannabinoid (CB) receptors and specific enzymes for endocannabinoid biosynthesis or catabolism in rat pineal glands and cultured pinealocytes. Immunohistochemical and immunoblot analyses revealed the presence of CB1 and CB2 receptor proteins, of N‐acyl phosphatidyl ethanolamine hydrolyzing phospholipase D (NAPE‐PLD), an enzyme catalyzing endocannabinoid biosynthesis and of fatty acid amide hydrolase (FAAH), an endocannabinoid catabolizing enzyme, in pinealocytes, and in pineal sympathetic nerve fibers identified by double immunofluorescence with an antibody against tyrosine hydroxylase. The immunosignals for the CB2 receptor, NAPE‐PLD, and FAAH found in pinealocytes did not vary under a 12 hr light:12 hr dark cycle. The CB1 receptor immunoreaction in pinealocytes was significantly reduced at the end of the light phase [zeitgeber time (ZT) 12]. The immunosignal for NAPE‐PLD found in pineal sympathetic nerve fibers was reduced in the middle of the dark phase (ZT 18). Stimulation of cultured pinealocytes with NE affected neither the subcellular distribution nor the intensity of the immunosignals for the investigated CB receptors and enzymes. In summary, the pineal gland comprises indispensable compounds of the endocannabinoid system indicating that endocannabinoids may be involved in the control of pineal physiology.
The role of polyamines in somatomedin‐stimulated differentiation of L6 myoblasts

The somatomedins are potent stimulators of proliferation and differentiation of cultured myoblasts. In studies on the mechanism(s) of these actions, we have measured the activities of ornithine decarboxylase (ODC), an enzyme associated with rapid cell proliferation, and creatine kinase (CK), a biochemical marker for muscle differentiation, after treatment of L6 myoblast cultures with Multiplication Stimulating Activity (MSA), a member of the somatomedin family of insulinlike growth factors. ODC levels reached a peak 24 hours after MSA addition (before any detectable differentiation of the myoblasts) and then decreased as differentiation commenced and CK activity increased. Addition of alpha‐difluoromethylornithine (DFMO), an irreversible inhibitor of ODC, caused a dramatic decrease in differentiation. Measurement of 3H‐thymidine incorporation, DNA content, and cell number established that the effect of DFMO on differentiation was not a simple consequence of its antiproliferative actions. Cellular levels of putrescine and spermidine (but not spermine) decreased substantially following addition of DFMO to the cultures. The inhibitory effects of DFMO were abolished upon addition of exogenous polyamines to the medium. However, addition of polyamines in the absence of MSA or DFMO did not mimic the stimulation of differentiation by MSA. We conclude that polyamines play an essential role in the stimulation of L6 myoblast differentiation by somatomedins, but they are not sufficient to effect this stimulation.
Collagen glycation and its role in fracture properties of bone.

Age-related non-traumatic fractures are a major public health problem. Although lower bone mass is the most commonly implicated variable for the age-related loss of bone strength and increased fracture incidence, recent evidence shows that the resistance of bone material against fracture (toughness) diminishes with age. The mechanisms for the age-related loss of toughness are, however, unknown. Bone derives its resistance against fracture from collagen deformation and its ability to form microcracks and uncracked ligament bridges during crack propagation. Collagen deformation, microcracking and uncracked ligaments are the primary toughening mechanisms in bone and any alteration in these toughening mechanisms will affect bone toughness. Using an in vitro ribosylation procedure, we demonstrate that the non-enzymatically (NEG) mediated accumulation of collagen cross-links stiffens the organic network reducing collagen deformation and measures of microcracking and crack bridging in bone. More significantly, NEG-induced collagen cross-links were highly correlated with the stiffness of the organic matrix in bone and the fracture properties of mineralized bone. Thus, NEGmediated stiffening of the organic matrix causes loss of bone toughness. Furthermore, as post-yield and damage behavior of cancellous bone are independent of bone volume fraction and similar to cortical bone, the organic matrix-mediated loss of toughness may be common to both cortical and cancellous bone. Consistent with this concept, the experiments conducted in our laboratory demonstrate that NEG induced cancellous bone fragility and that agerelated loss of toughness properties are similar in both cortical and cancellous bone. Acknowledgements
Region of Difference 4 in Alpine Mycobacterium caprae Isolates Indicates Three Variants

ABSTRACT The lack of complete genome sequence information for Mycobacterium caprae complicates a robust differentiation of M. caprae and Mycobacterium bovis. In this study, the presence or absence of M. caprae-specific single nucleotide polymorphisms in lepA and gyrB genes was assessed. The region of difference 4 (RD4) was analyzed for the identification and characterization of M. caprae. Molecular characteristics were evaluated in 12 recent M. caprae isolates from livestock and wildlife collected over a 3-year period in Bavaria, Germany. Conventional PCR strategies, sequence analysis of PCR fragments, and data from a next-generation sequencing approach together with variable-number tandem-repeat genotyping were utilized. Single nucleotide polymorphisms in the lepA and gyrB genes indicating the presence of M. caprae were detected in all the isolates. At least three different RD4 variants were found for Alpine M. caprae isolates. The results demonstrate that the RD4 region is rather heterogeneous in M. caprae genomes. As assumed by others, the presence of RD4 is critical for PCR-based differentiation of M. caprae from M. bovis, but in addition, the observed variability of RD4 allows the identification of M. caprae genotypes and may be indicative of a geographical-type appearance.
Free surface flow past topography: A beyond-all-orders approach

The problem of steady subcritical free surface flow past a submerged inclined step is considered. The asymptotic limit of small Froude number is treated, with particular emphasis on the effect that changing the angle of the step face has on the surface waves. As demonstrated by Chapman & Vanden-Broeck, (2006) Exponential asymptotics and gravity waves. J. Fluid Mech.567, 299–326, the divergence of a power series expansion in powers of the square of the Froude number is caused by singularities in the analytic continuation of the free surface; for an inclined step, these singularities may correspond to either the corners or stagnation points of the step, or both, depending on the angle of inclination. Stokes lines emanate from these singularities, and exponentially small waves are switched on at the point the Stokes lines intersect with the free surface. Our results suggest that for a certain range of step angles, two wavetrains are switched on, but the exponentially subdominant one is switched on first, leading to an intermediate wavetrain not previously noted. We extend these ideas to the problem of flow over a submerged bump or trench, again with inclined sides. This time there may be two, three or four active Stokes lines, depending on the inclination angles. We demonstrate how to construct a base topography such that wave contributions from separate Stokes lines are of equal magnitude but opposite phase, thus cancelling out. Our asymptotic results are complemented by numerical solutions to the fully nonlinear equations.
Differential yield of pathogens from stool testing of nosocomial versus community-acquired paediatric diarrhea.

OBJECTIVES To evaluate the role of routine stool examination for all pathogens in paediatric nosocomial diarrhea (NAD) and community-acquired diarrhea (CAD) over a two-year period at Alberta Children's Hospital and current practices in other Canadian hospitals. A secondary objective was to characterize features that may predict NAD or CAD etiology. STUDY DESIGN Retrospective cohort study and telephone survey. SETTING Alberta Children's Hospital (retrospective review) and Canadian tertiary care paediatric centres (telephone survey). METHODS The health and microbiological records of all children with an admission or discharge diagnosis of diarrhea were reviewed using a standardized data collection form. In addition, a telephone survey of laboratories serving all paediatric hospitals in Canada was conducted using a standard questionnaire to obtain information about practices for screening for pathogens related to NAD. RESULTS Four hundred and thirty-four CAD episodes and 89 NAD episodes were identified. Overall, rotavirus and Clostridium difficile were the most commonly identified pathogens. Bacterial culture was positive in 10.6% CAD episodes tested, with Escherichia coli O157:H7 identified as the most common non-C difficile organism. In NAD, no bacteria were identified other than C difficile (toxin). Screening for ova and parasites had negligible yield. Viruses were more frequent in the winter months, while bacterial pathogens were more common in the summer and fall months. Over 50% of Canadian paediatric hospitals still routinely process NAD specimens similarly to CAD specimens. CONCLUSIONS There is a need for the re-evaluation of routine ova and parasite screening, and bacterial culture in nonoutbreak episodes of NAD in children.
Coronaviruses: structure and genome expression.

Introduction. Progress in coronavirology is illustrated by the number of workshops convened and reviews written. International meetings have been held in Germany (1980), the Netherlands (1983) and the U.S.A. (1986), and the Fourth Coronavirus Symposium will be organized by one of us (D.C.) in Cambridge, U.K. in July 1989. In addition, reviews have appeared which highlighted particularly interesting characteristics of the family, e.g. the replication strategy (Lai, 1986) and the glycoproteins (Sturman & Holmes, 1985). As the last general accounts were published some 5 years ago (Siddell et al., 1983; Sturman & Holmes, 1983) an update is timely. The present article is based on the large amount of sequence data accumulated in these years and focuses on the viral nucleic acids and proteins and their function. Coronaviruses cause infections in man, other mammals and birds. Most experimental data have been obtained from studies of mouse hepatitis virus (MHV) and infectious bronchitis virus of chickens (IBV).
Review Paper: The Basis for Using the Internet to Support the Information Needs of Primary Care

Synthesizing the state of the art from the published literature, this review assesses the basis for employing the Internet to support the information needs of primary care. The authors survey what has been published about the information needs of clinical practice, including primary care, and discuss currently available information resources potentially relevant to primary care. Potential methods of linking information needs with appropriate information resources are described in the context of previous classifications of clinical information needs. Also described is the role that existing terminology mapping systems, such as the National Library of Medicine's Unified Medical Language System, may play in representing and linking information needs to answers.
MAC protocols design and a cross-layered QoS framework for next generation wireless networks. (Conception de protocoles de la couche MAC et modélisation des réseaux hétérogènes de nouvelle génération)

The present dissertation deals with the problem of under-utilization of collision channels and other related problems in wireless networks. It deals with the design of random access protocols for wireless systems and provides a mathematical framework for performance evaluation of multihop based heterogeneous wireless networks. This thesis is divided into three parts. In the first part, we propose new versions of slotted aloha incorporating power control, priority and hierarchy. Our simulations were important to understand the behaviour of such a system and the real impact of involved parameters (transmit power, transmit rate, arrival rate, hierarchy order). Both team problem (common objective function is maximized) and game problem (each user maximizes its own objective) were discussed. Introducing hierarchy seems to provide many promising improvement without/or with a low amount of external information. We also proposed two distributed algorithms to learn the desired throughput. Next, we developed in the second part an analytical Framework to evaluate performances of multihop based heterogeneous Wireless networks. We built a cross-layer model and derived expression of stability, end-to-end throughput and end-to-end delay. Furthermore, we provided an accurate approximation for the distribution of end-to-end delay in multihop ad hoc networks (operating with slotted aloha protocol). As a direct application, we highlighted how streaming and conversational flows could be supported in this class of ubiquitous networks. The third part of this thesis is devoted to understanding and modelling of IEEE 802.11e DCF/EDCF-operated multihop ad hoc networks. We indeed built a complete and simple APPLICATION/NETWORK/MAC/PHY cross-layered model with finite retries per packet per flow. We analyzed the stability of forwarding queues and derived expression of end-to-end throughput. We finally proposed a Fountain code-based MAC layer to improve the throughput/fairness over the network
Epitope spreading: protection from pathogens, but propagation of autoimmunity?

An epitope is an antigenic determinant, or a site on the surface of an antigenic molecule, to which a single antibody binds. Epitope spreading (ES) refers to the development of an immune response to epitopes distinct from, and noncross‐reactive with, the disease‐causing epitope. Diversification, or the ability of the immune system to attack multiple targets on a pathogen has obvious advantages. Here we review some of the evidence regarding its role in autoimmunity, in humans and in animal disease models. We consider the implications of ES on the development of highly specific therapies for autoimmune disease. We stress that pathogenic ES probably occurs in the context of inherent abnormalities in control mechanisms for the prevention of autoimmunity or other genetic predisposing factors.
Enteral Nutrient Supply for Preterm Infants: Commentary From the European Society of Paediatric Gastroenterology, Hepatology and Nutrition Committee on Nutrition

The number of surviving children born prematurely has increased substantially during the last 2 decades. The major goal of enteral nutrient supply to these infants is to achieve growth similar to foetal growth coupled with satisfactory functional development. The accumulation of knowledge since the previous guideline on nutrition of preterm infants from the Committee on Nutrition of the European Society of Paediatric Gastroenterology and Nutrition in 1987 has made a new guideline necessary. Thus, an ad hoc expert panel was convened by the Committee on Nutrition of the European Society of Paediatric Gastroenterology, Hepatology, and Nutrition in 2007 to make appropriate recommendations. The present guideline, of which the major recommendations are summarised here (for the full report, see http://links.lww.com/A1480), is consistent with, but not identical to, recent guidelines from the Life Sciences Research Office of the American Society for Nutritional Sciences published in 2002 and recommendations from the handbook Nutrition of the Preterm Infant. Scientific Basis and Practical Guidelines, 2nd ed, edited by Tsang et al, and published in 2005. The preferred food for premature infants is fortified human milk from the infant's own mother, or, alternatively, formula designed for premature infants. This guideline aims to provide proposed advisable ranges for nutrient intakes for stable-growing preterm infants up to a weight of approximately 1800 g, because most data are available for these infants. These recommendations are based on a considered review of available scientific reports on the subject, and on expert consensus for which the available scientific data are considered inadequate.
Reflections on the Abuse of Power, Control and Status in Group Therapy and Group Therapy Training

It is not by chance that recent papers by Rachman (2003a, b), Harwood (2003) and Moses (2003) refer to sociocultural, religious, political, and historical perspectives on power and its misuse. Issues of power and the allied concept of leadership, and the potential abuse of power and leadership, are central aspects of human life. We attend to them no less today than at any other point in our history. The critical questions raised by these kinds of papers are: What are the determinants of, the effects of, and the safeguards against the misuse of power by those who would practice and teach group therapy? Central to Rachman’s paper is Sandor Ferenczi’s (1949) conceptualization of the traumatogenic effects of the “confusion of tongues,” a formulation echoed in the papers by Harwood and Moses. Ferenczi describes the two languages that are spoken in the development of the child—the child’s and the parent’s—just
p204-Initiated Innate Antiviral Response in Mouse Leydig Cells1

ABSTRACT The mammalian testis is an immunoprivileged organ where local tissue-specific cells acquire an effective innate immune function against invading microbial pathogens. The present study demonstrated that mouse Leydig cells had innate antiviral activities in response to viral DNA challenge through p204 activation. The DNA sensor p204 and its signaling adaptor stimulator of interferon (IFN) genes (STING) were constitutively expressed in Leydig cells. Synthetic herpes simplex virus DNA analog (HSV60), a p204 agonist, induced the expression of type I IFNs and various antiviral proteins, including IFN-stimulating gene 15, 2′5′-oligoadenylate synthetase, and Mx glutamyl transpeptidase 1, in Leydig cells. The HSV60-induced innate antiviral response in Leydig cells was significantly reduced by inhibiting p204 signaling using specific small interfering RNAs targeting p204 and Sting. The antiviral response did not affect steroidogenesis in Leydig cells. These results indicated a novel mechanism underlying the testicular innate antiviral response.
Epigenetic Reprogramming by Somatic Cell Nuclear Transfer in Primates

We recently demonstrated that somatic cells from adult primates could be reprogrammed into a pluripotent state by somatic cell nuclear transfer. However, the low efficiency with donor cells from one monkey necessitated the need for large oocyte numbers. Here, we demonstrate nearly threefold higher blastocyst development and embryonic stem (ES) cell derivation rates with different nuclear donor cells. Two ES cell lines were isolated using adult female rhesus macaque skin fibroblasts as nuclear donors and oocytes retrieved from one female, following a single controlled ovarian stimulation. In addition to routine pluripotency tests involving in vitro and in vivo differentiation into various somatic cell types, primate ES cells derived from reprogrammed somatic cells were also capable of contributing to cells expressing markers of germ cells. Moreover, imprinted gene expression, methylation, telomere length, and X‐inactivation analyses were consistent with accurate and extensive epigenetic reprogramming of somatic cells by oocyte‐specific factors. STEM CELLS 2009;27:1255–1264
Genetic Basis of Antibiotic Resistance in Streptococcus agalactiae Strains Isolated in a French Hospital

ABSTRACT The genetic basis of antibiotic resistance in 113 unrelated group B streptococci was studied by PCR. Ninety-four strains were resistant to tetracycline-minocycline, and tet(M) was detected in 85% of these isolates. Seventeen erythromycin-resistant strains contained the erm(B), erm(TR), or mef(A) gene. Eleven strains exhibited high-level resistance to kanamycin due to the presence of the aphA3 gene; eight of these strains were also highly resistant to streptomycin; aad-6-related sequences were detected in seven strains.
The Hospital Discharge: A Review of a High Risk Care Transition With Highlights of a Reengineered Discharge Process

The hospital discharge is a handoff, ripe embedded structural risks and hazards that can result in passive or active failures among "sharp end" providers. These failures can result in medical errors and an array of postdischarge adverse events. There are now emerging data to suggest that postdischarge-related adverse events and rehospitalizations can be reduced through interventions at the time of hospital discharge. This article reviews the modifiable components of the hospital discharge process related to adverse events and rehospitalizations, including those relating to the characteristics of the hospital, patient, and clinician. Using multimethod analysis, our group described the principles thought to be important to the discharge process and delineated what we now call the reengineered discharge, a set of 11 discrete and mutually reinforcing components that we believe should be consistently part of every hospital discharge. Finally, we discuss the work or the National Quality Forum Consensus Standards Maintenance committee who, in 2006, added the hospital discharge as one of its "safe practices for better healthcare. "
Sleep Habits and Sleep Disturbance in Elementary School‐Aged Children

&NA; Relatively little is known about sleep habits, sleep disturbances, and the consequences of disordered sleep in school‐aged children. This descriptive study examined a variety of common sleep behaviors in a group of 494 elementary school children, grades kindergarten through fourth, using a battery of sleep questionnaires that included parent, teacher, and self‐report surveys. The prevalence of parent‐defined sleep problems ranged from 3.7% (Sleep‐Disordered Breathing) to 15.1% (Bedtime Resistance), with 37% of the overall sample described as having significant sleep problems in at least one sleep domain. Younger children were more likely than older children to have sleep problems noted by parents (particularly bedtime struggles and night wakings), as well as by teacher and self‐report. Children tended to identify more sleep problems by self‐report, particularly sleep‐onset delay and night wakings, than did their parents. Overall, approximately 10% of the sample was identified by all three measures as having significant problems with daytime sleepiness. The results of this study emphasize the importance of screening for sleep disorders in this age group in the clinical setting. The need for consensus regarding the use of sleep screening instruments and the definition of “problem” sleep in school‐aged children is also discussed.