Miracle-dz/UniFAR-FTUs huggingface dataset:
The effectiveness of the Madufor® feed additive in hyperthermia conditions for broiler chickens

Among the severe lightning-fast pathological processes that affect farm animals and poultry of all age categories, it is worthwhile to highlight overheating and dehydration, which are especially common in the southern regions of the country. The paper presents the results of studies on the effectiveness of Madufor® feed additives in heat stress of broiler chickens. According to the results of hematological studies, it was found that the content of red blood cells, hemoglobin, hematocrit and platelets in the blood of chickens of the control group was at the upper boundary of the physiological norm or slightly exceeded them. Thus, the level of red blood cells in the control group was 4.18·1012/l, hemoglobin 107.11g/l, hematocrit 39.32% and platelets 232.85·109/l, and in the experimental group these indicators were within the physiological norm and amounted to 3.47·1012/l, 103.89g/l, 34.48% and 168.85·109/l, respectively, which indicates the normalization of metabolic processes in the body of broiler chickens under the influence of the Madufor® feed additive. In the blood serum of broiler chickens in the control group, an increased total protein content of 5.28g/l (11.91%; P<0.01) was compared with the experimental group. The AST level in the control group also exceeded the similar values of the experimental group by 15.06 units/l at P<0.01. During the experiment, the mineral composition of the blood serum of the chickens of the experimental group improved in relation to the control. The concentration of calcium, sodium and potassium significantly exceeded the control by 14.78 (P<0.05), 1.65 (P<0.05) and 8.15% (P<0.01), and the level of phosphorus tended to increase with an unreliable difference. Throughout the cultivation, the live weight of the chickens of the experimental group exceeded the control values. During the finishing fattening of broilers of the experimental group at the age of 28 and 35 days, their live weight exceeded the same indicator in the control group by 171.7 (13.06%; P<0.001) and 288.2 g (15.42%; P<0.001 ) Accordingly, feed consumption per 1 kg of growth in the control group was significantly higher than in the experimental group by 0.26 kg.
An Updated Review of Pharmacological, Standardization Methods and Formulation Development of Rutin

1Rashid Latif College of Pharmacy, 35-kmFerozepur Road, Lahore, Pakistan. 2University College of Pharmacy, University of the Punjab, Lahore, Pakistan. 3School of Bioprocess Engineering, Universiti Malaysia Perlis (UniMAP), Kompleks Pusat Pengajian Jejawi 3 (KPPJ3), Kawasan Perindustrian Jejawi, 02600, Arau, Perlis, Malaysia. 4Department of Physics, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia. 5Saint-Petersburg State Chemical-Pharmaceutical Academy, Ministry of Healthcare of the Russian Federation, 14, ul. Prof. Popova, St. Petersburg, 197376 Russia.
Predictive role of socio-demographic and chronotype on health-related quality of life of cancer patients from southeastern India

ABSTRACT It is well known that cancer and its treatment produce marked impact on the health-related quality of life (HRQoL) of cancer patients. Research concerning impact of chronotype on HRQoL in cancer patients is almost not studied yet, but the interests are growing in several diseases. Present study was carried out to explore the impact of socio-demographics, chronotype and consumption of tobacco, alcohol and sleeping medicine on HRQoL of Indian oncology patients. Self-reported Quality-of-Life questionnaire (EORTC QLQ-C30), Hospital Anxiety and Depression Scale (HADS), and Morningness-Eveningness Questionnaire (MEQ) were administered to the cancer patients (N = 1000) in the native Hindi language. Results revealed that among the socio-demographic factors, only age exhibited significant negative association with physical, role and cognitive functioning and positive association with symptoms, namely fatigue and pain. Interestingly, chronotype was found to be positively associated with emotional functioning and negatively with nausea-vomiting, dyspnoea, diarrhoea and depression. Patients who consumed tobacco, alcohol or sleeping medicine exhibited lower functioning and higher symptoms. Further, treatment of cancer also produced effect on a few measures of HRQoL of patients. In conclusion, age, chronotype and consumption of tobacco, alcohol or sleeping medicine were found to be important determinants of HRQoL of the patients.
Anti-establishment coalition governments in Southern Europe: Greece and Italy

Anti-establishment parties have been entering contemporary European Democracies with sizeable vote shares. During the Great Recession, the Greek and the Italian party system could be perceived as convergent case-studies for the formation and breakthrough of anti-establishment parties. Given the fact that ideologically diverging anti-establishment parties – the Coalition of the Radical Left - Social Unionist Front (SYRIZA) and the Independent Greeks (ANEL) in the Greek case, as well as the Five Star Movement (M5S) and the League in the Italian one – came to power and formed coalition governments, the primary goal of this article is to enquire into supply-side parameters, exploring potential associations along a range of programmatic stances and policy dimensions that effectuated these governing alliances. Using the Comparative Manifesto Project dataset, our findings confirm the existence of expected programmatic differences as well as a converging policymaking basis between the anti-establishment coalition partners of both governing alliances.
Teaching with Digital 3D Models of Minerals and Rocks

We use a small photography set-up consisting of a 30 cm-across, square, white, plastic Lazy-Susan turntable, a 50 cm-across, cubic, fabric lightbox with three LED ring lights, and an 18.4 MP Sony DSC-HX80 compact digital camera mounted on a simple tripod. We use a matte black background in the lightbox with pastel white filters on the LED lights. The turntable is marked-up with pairs of colored dots in the corners and approximately halfway towards the center; these are used to assist manual registration of images. Samples up to 40 cm-diameter can be accommodated but we generally restrict ourselves to samples less than 25 cm-diameter to enable use of the registration marks on the turntable.
Flood Prediction Using IoT Enabled Sensor Network and Machine Learning

Floods are one of the most common natural disasters that affects humanity. Unless we take the proper precautions, it will endanger human life itself. Flood prediction using IoT enabled sensor network and machine learning outlines a very efficient way to properly understand and manage floods. This technology can be used to evaluate the risk of flooding, there by speeding up precautionary measures. IoT is a network of computing devices with unique identifiers which helps transferring data across the devices without interaction between human and computers. By using different sensors, information about various environmental conditions were collected and transfer it to google sheet with the help of IoT technology. The client can analyse the data in the form of dataset from a remote device and predict the risk of flood.
KONSENTRASI EFEKTIF DAYA ANTIBIOFILM KITOSAN CANGKANG UDANG TERHADAP STREPTOCOCCUS VIRIDANS

Background.Periapical tooth infection is one of infection problems which often happens such as abscess periapical which is caused by bacteria. The bacteria which can form biofilms is named streptococcus viridans. It is more resistant than an antibacterial agent. Chitosan made of shrimp shells is used as a natural antibiofilms agent for streptococcus viridans. Purpose. To determine the effective concentration of antibiofilms capacity from shrimp shells chitosan towards streptococcus viridans. Method.The research method used in this research is laboratory experimental research. The research design is post-test only control design. Streptococcus viridans is given vortex until it becomes homogeneous with standard turbidity McFarland of 0.5, consequently, it is planted inside a microtitter plate using TSB Glu for 5x24 hours. At last, Streptococcus viridans is colored using crystal violet and the picture of biofilms is observed using inverted microscope. Chitosan liquid diluted through various concentration 0.195%, 0.39%, 0.78%, 1.56%, 3.125%, 6.25%, 12.5%, 25%, 50%, and 100% are going to be added to the microtitter plate and being incubated for 24 hours. The interpretation of the result on the longitude of the wave through optical density is 570nm. Result.There is a significant difference between the concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78%, and 0.39% and the control group. Chitosan’s effective concentration in resisting the biofilms is 50%. The result is determined by statistical analysis. Conclusion.The effective concentration to resist the formation of Streptococcus viridans biofilms using shrimp shells chitosan is 50%.
Analog Signalling with ‘Digital’ Molecular Switches

Molecular switches, such as the protein kinase CaMKII, play a fundamental role in cell signalling by decoding inputs into either high or low states of activity; because the high activation state can be turned on and persist after the input ceases, these switches have earned a reputation as ‘digital’. Although this on/off, binary perspective has been valuable for understanding long timescale synaptic plasticity, accumulating experimental evidence suggests that the CaMKII switch can also control plasticity on short timescales. To investigate this idea further, a non-autonomous, nonlinear ordinary differential equation, representative of a general bistable molecular switch, is analyzed. The results suggest that switch activity in regions surrounding either the high- or low-stable states of activation could act as a reliable analog signal, whose short timescale fluctuations relative to equilibrium track instantaneous input frequency. The model makes intriguing predictions and is validated against previous work demonstrating its suitability as a minimal representation of switch dynamics; in combination with existing experimental evidence, the theory suggests a multiplexed encoding of instantaneous frequency information over short timescales, with integration of total activity over long timescales. Author Summary Bistable molecular switches can decode cellular inputs into distinct high- or low-states of persistent enzymatic activity. Although this on-off, ‘digital’ perspective is valuable for long timescales, I suggest that short timescale fluctuations of switch activity around either stable state acts as an analog signal that reliably encodes instantaneous input frequency. A minimal model and theory make predictions about the molecular switch CaMKII, synaptic plasticity and burst detection.
The experimental program at IHEP of CAS

The Institute of High Energy Physics (IHEP) is a comprehensive research center for the study of particle physics in China. It hosts or manages a number of key China based projects in operation or in preparation, and plays a major role in planning future research in the field. The rearch program at IHEP covers from the underground reactor neutrino experiments, collider experiments, high mountain air shower experiments, to space missions. In this note a few selected experiments are introduced.
Cerebellar granule cell precursors can extend processes, undergo short migratory movements and express postmitotic markers before mitosis in the chick EGL

Cerebellar granule cell precursors (GCPs) form a secondary germinative epithelium, the external germinal layer (EGL) where they proliferate extensively to produce the most numerous cell type in the brain. The morphological sequence of events that characterizes the differentiation of GCPs in the EGL is well established. However, morphologies of individual GCP and their differentiation status have never been correlated. Here, we examine the morphological features and transitions of GCPs in the chicken cerebellum by labelling a subset of GCPs with a stable genomic expression of a GFP transgene and following their development within the EGL in fixed tissue and using time-lapse imaging. We use immunohistochemistry to observe cellular morphologies of mitotic and differentiating GCPs to better understand their differentiation dynamics. Results reveal that mitotic activities of GCPs are more complex and dynamic than currently appreciated. While most GCPs divide in the outer and middle EGL, some are capable of division in the inner EGL. Some GCPs remain mitotically active during process extension and tangential migration and retract their processes prior to each cell division. The mitotically active precursors can also express differentiation markers such as TAG1 and NeuroD1. Further, we explore the result of misexpression of NeuroD1 on granule cell development. When misexpressed in GCPs, NeuroD1 leads to premature differentiation, defects in migration and reduced cerebellar size and foliation. Overall, we provide the first characterisation of individual morphologies of mitotically active cerebellar GCPs in ovo and reaffirm the role of NeuroD1 as a differentiation factor in the development of cerebellar granule cells.
Strange Stable Replicators Generated From Mumps Virus cDNA Clones

In reverse genetic experiments we have isolated recombinant mumps viruses (rMuV) based on a recent clinical isolate that carry large numbers of mutations clustered in small parts of their genome and which are not caused by biased hyper-mutation. In two separate experiments we obtained such rMuV: one virus had 19 mutations in the V/P region of the genome; the other, which also contained an extra transcription unit encoding green fluorescent protein (EGFP), had 32 mutations in the N gene. These specific constellations of mutations have not been observed in naturally occurring MuV isolates. The vast majority of the mutations (48/51) are synonymous. On passage in Vero cells and human B-LCL cells, a B lymphocyte-like cell Line, these mutations appear stable as no reversal occurs to the original consensus sequences, though mutations in other genes occur and change in frequency during passage. Defective Interfering RNAs accumulate in passage in Vero cells but not in B-LCL cells. Interestingly, in all passaged samples the level of variation in the EGFP gene is the same as in the viral genes, though it is unlikely that this gene is under any functionality constraint. The stability in repeated high multiplicity passage indicates that the constellation of mutations is placing the virus on a fitness peak from which it cannot escape. What mechanism gave rise to these mutant viruses and their stability remain open questions of interest to a wider field than mumps reverse genetics alone.
c-kit D816V Provides a Strong Signal for Myelomastocytic Differentiation and Cluster Formation in Murine Ba/F3 Cells.

The pathologic hallmark of systemic mastocytosis (SM) is differentiation and cluster formation of mast cells (MC) in hematopoietic tissues. The somatic c-kit mutation D816V is detectable in a majority of all SM patients independent of the proliferation-status of MC or subtype (indolent or aggressive) of disease. To investigate the role of c-kit D816V in the pathogenesis of SM, we established a Ba/F3 cell line with doxycycline-inducible expression of c-kit D816V. We found that c-kit D816V provides a strong signal for mast cell differentiation and cluster formation in Ba/F3 hematopoietic progenitor cells without enhancing their growth thereby resembling the clinical presentation of indolent SM (ISM). As assessed by gene chip analysis, induction of c-kit D816V resulted in expression of various differentiation antigens including mouse mast cell protease 5, mi transcription factor, histidine decarboxylase (HDC), secretory granule proteoglycan, IL-4 receptor, ICAM-1, and CD63 consistent with an early phase of mastopoiesis. By contrast, c-kit D816V did neither induce expression of granulo-monocytic antigens such as myeloperoxidase, IL-3 receptor, or GM-CSF receptor, nor expression of ‘late stage’ mast cell antigens such as FceRI. The c-kit D816V-induced synthesis of histamine in Ba/F3 cells was confirmed by RIA. To examine the role of c-kit D816V in the pathogenesis of mastocytosis, we extended our analysis to bone marrow biopsy sections obtained from patients with ISM. In these experiments, the D816V-mutated form of c-kit was detected more frequently in micro-dissected tryptase-positive MC obtained from dense compact infiltrates (44.2%) than in diffusely spread MC in these patients (22.6%) (p
Validation of molecular marker AVRDC-PP12 linked to male sterility gene ms10 of chilli

The newly identified SSR marker AVRDC-PP12 linked to the male sterility gene ms10 distinguished the heterozygous (Ms10ms10) fertile from the homozygous (Ms10Ms10) fertile plants in chilli segregating populations. To assess efficiency of AVRDC-PP12 in marker-assisted backcrossing for transfer of ms10 gene; the marker was screened in four backcross progenies. The marker co-segregated with ms10 gene in three progenies derived from the crosses ‘MS-12 × VR-16’, ‘MS-12 × S-217621’ and ‘MS-12 × Selection Dev’ with recombination frequency of 3.22, 4.16 and 3.27%, respectively. The marker failed to differentiate the parents in the cross ‘MS-12 × DCL-524’and hence was not used for further genotyping of the population.
Modulation of Ã-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors by a novel organic nitrate ester

Positive modulators of the excitatory amino acid receptor a-Amino-3hydroxy-5-methyl-4-isoxazole Propionate (AMPA) has been shown to enhance memory and learning tasks performed by rodents and by human subjects. A new class of AMPA receptor modulators has been recently synthesized. These compounds belong to the class of organic nitrate ester of the nitroglycerine family and are designated GT-2 1-005, GT-11-013, GT-21-038, and GT-21-022. GT-2 1 005 was found to enhance AMPA receptor-mediated responses. The structure of this drug is simple compared to the various positive modulators of the AMPA receptor that have already characterized. GT-21-005 en hanced kainate-evoked currents in cultured and acutely isolated hippocampal neurons. It also enhanced glutamate-evoked currents by reducing desensitization at the receptor and increasing the rate of recovery from desensitization. The presence of GT-21-005 also prolonged the decay of spontaneous miniature excitatory postsynaptic currents (mEPSCs). This shows that GT-21-005 can enhance synaptic responses that may be responsible for encoding rnemory and learning in the hippocampus. GT-21-005 also has inhibitory effects on N-rnethyl-D-aspartate (NMDA) and y-am inobutryic acid (GABA) receptors in the same concentrations that enhance AMPA receptors. This thesis reports the characterization of the effects of GT-21-005 on AMPA receptors using electrophysiological techniques on cultured and acutely isolated neurons from the hippocampus. Acknowledaments I would like to express rny sincere thanks to Dr. John F. MacDonald for his patience and support in rny project. His insightful wisdom and knowledge has been rewarding in my learning experience with this lab. To Dn. Greg Thatcher and James Reynolds, I thank them for their collaboration and support in ! his project. I would also like to thank Drs. Zhigang Xiong and Donglin Bai for their friendship and contributions to this project. I also thank Dr. Peter Pennefather for his support as my Graduate Advisor and for enlightening me in kinetic modeling. For Drs. Beverley Oner, WeiYang Lu, Stefanie Roder, and Mike Jackson, I am grateful for their discussions and friendship. As well, I would like to thank my Examination Cornmittee (Drs. J.F. MacDonald, A. Okey, B. Orser, P. Pennefather, and H. Van Tol) for their attendance at my defense, the day snow actually shut the University down. I would also like to thank my parents from the bottom of my heart for al1 their love and support during al1 my years o f study. TABLE OF CON1 EN1 S Abstract . i . Acknowledgments . 11 . Table Of
Wet bulb and Conilon coffee root distribution under drip irrigation

Knowledge of the wet bulb and the root system of the Conilon coffee plant is highly important for the correct management of irrigation. Therefore, the aim of this work was to characterize the wet bulb and Conilon coffee root distribution under drip irrigation. The experiment was conducted in the city of São Mateus, ES, Brazil with five replications of a completely randomized design of a 4 x 6 split-plot scheme, which represents four points located according to plant location and six depths. Two points were located in the plant line and two points between lines. For row spacing, we used a split-plot scheme 5 x 6 with five points in relation with plant location and six depths with five replications. The coffee roots were analysed by volume, superficial area, length and diameter. The wet bulb was measured with tubes located in six points near the plants with two points in the plant row (between two plants) and five points between rows. The measurements were conducted at four depth ranges with three replications. The wet bulb reached a depth of between 0.40 and 0.50 m, providing an excess of water in depth, evidencing the importance of this characterization for the irrigation management of drip-irrigated Conilon coffee. The depth of the radicular system for better irrigation management efficiency of drip-irrigated coffee is 0.30 m, exhibiting 67.4% of root volume and 68.0% of surface area.
Evolution of anisogamy in the early diverging fungus, Allomyces

Gamete size dimorphism between sexes (anisogamy) is predicted to have evolved from an isogamous system in which sexes have equal-sized, monomorphic gametes. Although adaptive explanations for the evolution of anisogamy abound, we lack comparable insights into molecular changes that bring about the transition from monomorphism to dimorphism. The basal fungal clade Allomyces provides unique opportunities to investigate genomic changes that are associated with this transition in closely related species that show either isogamous or anisogamous mating systems. The anisogamous species show sexual dimorphism in gamete size, number, pigmentation and motility. We sequenced transcriptomes of five Allomyces isolates representing the two mating systems, including both male and female phenotypes in the anisogamous species. Maximum likelihood ancestral character state reconstruction performed in MESQUITE using the de-novo assembled transcriptomes indicated that anisogamy likely evolved once in Allomyces, and is a derived character as predicted in theory. We found that sexual stages of Allomyces express homologs of several genes known to be involved in sex determination in model organisms including Drosophila and humans. Furthermore, expression of CatSper homologs in male- and female-biased samples in our analysis support the hypothesis that gamete interaction in the anisogamous species of Allomyces may involve similar molecular events as the egg-sperm interaction in animals, including humans. Although the strains representing either mating system shared much of the transcriptome, supporting recent common ancestry, the analysis of rate of evolution using individual gene trees indicates high substitution rates and divergence between the strains. In summary, we find that anisogamy likely evolved once in Allomyces, using convergent mechanisms to those in other taxa.