Miracle-dz/UniFAR-FTUs huggingface dataset:
Induced Hippocampal Neuron Protection in an Optimized Gerbil Ischemia Model: Insult Thresholds for Tolerance Induction and Altered Gene Expression Defined by Ischemic Depolarization

Preconditioning of hippocampal CA1 neurons was evaluated in a gerbil model of transient global ischemia using extracellular recording of DC potential shifts characteristic of ischemic depolarization to precisely define the duration of both priming and test insults. Brief ischemia resulting in depolarizations of 2.5 to 3.5 minutes consistently induced maximal tolerance (95% protection) against subsequent challenges 2 days later with an approximate doubling of the insult duration required for complete CA1 neuron loss from 6 to 12 minutes depolarization when evaluated 1 week after the test insult. Significant protection persisted at 2 months survival, although the apparent injury threshold regressed to approximately 8 minutes, indicating delayed progression of injury after longer test insults. In situ hybridization was used to evaluate depolarization thresholds for induction of mRNAs encoding the 70 kDa heat shock/stress protein, hsp72, as well as several immediate-early genes (c-fos, c-jun, junB, and junD). Immediate-early genes were prominently expressed after short insults inducing tolerance, whereas appreciable hsp72 induction only occurred after insults approaching the threshold for neuron injury. These results establish an ischemic preconditioning model with the predictability needed for mechanistic studies and demonstrate that prior transcriptional activation of the postischemic heat shock response is not required for expression of delayed tolerance.
Studying transmission of fuel storage bank to NGV cylinder in CNG fast filling station

The exact modeling of the fast-fill process of Compressed Natural Gas (CNG) fueled storage bank occurring to Compressed Natural Gas Vehicle (NGV) cylinders is an unintelligible process, and should be thoroughly studied. In this paper, a theoretical model based on mass balance and thermodynamic laws has been developed to study dynamic fast filling process of CNG storage bank to vehicle's (NGV) cylinder. Because Methane occupies a large percentage (between 70% to 99%) of natural gas, for the sake of simplicity it is assumed that Methane is the only substance in Natural gas and thermodynamic properties table has been employed for case of real gas model based on methane. For modeling the heat transfer, the system has been treated as an adiabatic lump one. The result shows the initial pressure of storage bank has a big effect on the storage bank volume for bringing up the NGV cylinder to its target pressure (20 MPa). The storage bank volumes required for bringing up the NGV cylinder to its final (target) pressure (20 MPa) for various initial storage bank pressure, like 20.8 MPa (RPS = 1.04), 23 MPa (RPS = 1.15) and 25 MPa (RPS = 1.25) are respectively 22, 6 and 4 times the NGV cylinder volume. It is noted that RPS is the ratio of storage bank pressure (PS) to target pressure (PT) (In this research is 20 MPa). The results also showed that ambient temperature has a big effect on refueling process, chiefly on final NGV cylinder and storage bank conditions.
Varied effects of atypical neuroleptics on P50 auditory gating in schizophrenia patients.

OBJECTIVE Sensory gating deficits found in schizophrenia can be assessed by using a paired auditory stimulus paradigm to measure auditory evoked response. The ratio of the P50 response amplitude of the second or test stimulus to that of the first or conditioning stimulus is expressed as a percentage. Normal subjects generally suppress the second response and typically have ratios of less than 40%. Subjects with schizophrenia and half their first-degree relatives have deficits in sensory gating, with P50 ratios that are generally greater than 50%. Treatment with typical neuroleptics does not reverse this deficit. However, previous studies have shown that treatment with clozapine, an atypical neuroleptic, ameliorates this deficit in clinically responsive patients. This study sought to determine whether other atypical neuroleptics improve P50 ratios. METHOD P50 evoked potential recordings were obtained from 132 patients with schizophrenia and 177 healthy comparison subjects. Eighty-eight patients were being treated with atypical neuroleptics (clozapine [N=26], olanzapine [N=31], risperidone [N=22], and quetiapine [N=9]). Thirty-four patients were taking typical neuroleptics, and 10 were unmedicated. RESULTS Healthy subjects exhibited P50 suppression that was significantly better than the schizophrenia patients receiving typical neuroleptics (mean=19.8% [SD=21.0%] versus 110.1% [SD=87.9%]). Patients receiving atypical neuroleptics had a mean P50 ratio that fell between these two means (mean=70.4%, SD=53.7%). When patients treated with different atypical neuroleptics were compared, only the clozapine group had mean P50 ratios that were in the normal range. All other groups exhibited auditory P50 response inhibition that was significantly poorer than that of the healthy subjects. CONCLUSIONS Improvement in P50 gating appears to be greatest in patients treated with clozapine.
A study of the nutritive value of Toprina G in the diet of two hybrid strains of caged laying hens.

An experiment is reported in which the hydrocarbon-grown yeast Toprina G was used at levels of 0, 20, 40, 60, 80, 100 and 120 g kg−1 of diet to replace mainly soybean meal in a fishmeal-free control diet fed to two lightweight hybrid strains of caged layers. The Toprina G had a very low Se content (14 μg kg-−1) and a high Mn content (325 mg kg−1). Replacement of the soy-bean meal of the control diet and also, at the highest level of Toprina G addition, the meat and bone meal by the hydrocarbon-grown yeast did not statistically affect total egg weight or mean egg weight significantly (total egg weight/total number of eggs laid). Total food intake tended to be higher for the birds fed the diets containing Toprina G although for the full experimental period this effect was not statistically significant. For one of the hybrid strains the food conversion efficiency was poorer for all the treatments receiving Toprina G than for the control. This effect was statistically significant at the 60 and 100 g Toprina G inclusion per kg of diet. The results indicate that Toprina G may be used as the major protein source in the diet of caged layers up to a level of 12 %.
DRUG-DRUG INTERACTION BETWEEN PITAVASTATIN AND VARIOUS DRUGS VIA OATP1B1

It has already been demonstrated that pitavastatin, a novel potent HMG-coenzyme A reductase inhibitor, is taken up into human hepatocytes mainly by organic anion transporting polypeptide (OATP) 1B1. Because OATP2B1 is also localized in the basolateral membrane of human liver, we took two approaches to further confirm the minor contribution of OATP2B1 to the hepatic uptake of pitavastatin. Western blot analysis revealed that the ratio of the band density of OATP2B1 in human hepatocytes to that in our expression system is at least 6-fold lower compared with OATP1B1 and OATP1B3. The uptake of pitavastatin in human hepatocytes could be inhibited by both estrone-3-sulfate (OATP1B1/OATP2B1 inhibitor) and estradiol-17β-d-glucuronide (OATP1B1/OATP1B3 inhibitor). These results further supported the idea that OATP1B1 is a predominant transporter for the hepatic uptake of pitavastatin. Then, to explore the possibility of OATP1B1-mediated drug-drug interaction, we checked the inhibitory effects of various drugs on the pitavastatin uptake in OATP1B1-expressing cells and evaluated whether the in vitro inhibition was clinically significant or not. As we previously reported, we used the methodology for estimating the maximum unbound concentration of inhibitors at the inlet to the liver (Iu,in,max). Judging from Iu,in,max and inhibition constant (Ki) for OATP1B1, several drugs (especially cyclosporin A, rifampicin, rifamycin SV, clarithromycin, and indinavir) have potentials for interacting with OATP1B1-mediated uptake of pitavastatin. The in vitro experiments could support the clinically observed drug-drug interaction between pitavastatin and cyclosporin A. These results suggest that we should pay attention to the concomitant use of some drugs with pitavastatin.
Operational experience with state estimation at Hydro-Québec

This paper first relates the experience gained with the state estimation function at Hydro-Quebec, experience that started with an in house development in the early 70psilas and was pursued with the acquisition of two commercial products, the latest one commissioned in early 2007. Subjects such as model building and conversion, system integration and commissioning, are addressed here. Next, the paper describes the Hydro-Quebecpsilas state estimation model and presents some of the practical problems encountered with the function. Finally, the authors conclude on the more important role that the state estimation function will have to play at Hydro-Quebec in the future. Topology errors, model inaccuracies, measurement system, solution quality and new advanced optimization functions are among the issues discussed.
Antiserum‐mediated demyelination: Relationship between remyelination and functional recovery

A focal demyelinative lesion of peripheral nerve was produced by intraneural injection of either antiserum from rabbits with experimental allergic neuritis or experimental allergic encephalomyelitis or antiserum to galactocerebroside. We studied the relationship between clinical and electrophysiological recovery from this lesion and the morphological pattern of remyelination. Foot muscles on the injected side weakened within an hour of injection and remained paralyzed for 7 days; strength gradually returned to normal by 16 days after injection. Electrophysiological conduction block, apparent within a few hours of injection, persisted for about 7 days. At 8 days we detected dispersed, very low amplitude muscle action potentials with long latency. Morphologically, demyelinated axons were surrounded by Schwann cells at 7 days after injection, but compacted myelin was not present. After 8 days, remyelinating axons became surrounded by thickening compacted myelin. The time of onset of remyelination and the rate of remyelination up to 14 days following the injection were independent of axon size. The onset of clinical and electrophysiological recovery from the lesion corresponded to the appearance of 2 to 8 myelin lamellae around each remyelinating axon. At 37 days after injection, when conduction velocities had returned to preinjection values, myelin thickness of remyelinating fibers had increased to approximately one‐third that of control nerves.
Unexpected consequences of increasing CO2 and ocean acidity on marine production of DMS and CH2ClI: Potential climate impacts

Increasing atmospheric mixing ratios of CO2 have already lowered surface ocean pH by 0.1 units compared to preindustrial values and pH is expected to decrease an additional 0.3 units by the end of this century. Pronounced physiological changes in some phytoplankton have been observed during previous CO2 perturbation experiments. Marine microorganisms are known to consume and produce climate‐relevant organic gases. Concentrations of (CH3)2S (DMS) and CH2ClI were quantified during the Third Pelagic Ecosystem CO2 Enrichment Study. Positive feedbacks were observed between control mesocosms and those simulating future CO2. Dimethyl sulfide was 26% (±10%) greater than the controls in the 2x ambient CO2 treatments, and 18% (±10%) higher in the 3xCO2 mesocosms. For CH2ClI the 2xCO2 treatments were 46% (±4%) greater than the controls and the 3xCO2 mesocosms were 131% (±11%) higher. These processes may help contribute to the homeostasis of the planet.
Performance of an HMM speech recognizer using a real-time tracking microphone array as input

This correspondence reports results for a tracking, real-time microphone array as an input to a hidden Markov model based (HMM-based) connected alpha-digits speech recognizer. For a talker in the near field of the array (within 0.5 m), performance approaches that of a close-talking microphone input device.
PATIENTS WITH MODERATE HEAD INJURY: A PROSPECTIVE MULTICENTER STUDY OF 315 PATIENTS

OBJECTIVETo analyze the risk factors of worst outcome associated with moderate head injury. METHODSData on patients with moderate head injury were collected prospectively in 11 Italian neurosurgical units over a period of 18 months. Patients older than 18 years with blunt head injury and at least one Glasgow Coma Scale (GCS) score between 9 and 13 were enrolled. The outcome was determined at 6 months using the Glasgow Outcome Scale. RESULTSWe analyzed 315 patients. Initial computed tomographic scans showed a diffuse injury type I or II in 63%, a mass lesion in 35%, and traumatic subarachnoid hemorrhage in 42% of the patients. The risk of progression toward a mass lesion was 23% when the admission computed tomographic scan showed diffuse injury type I or II. An emergency craniotomy was performed in 22% of the patients, delayed surgery was performed in 14%, and both were performed in 25%. A favorable outcome was obtained in 74% of the patients. When the GCS score was 9 or 10, the predictor of worst outcome was a motor GCS score of 4 or lower (odds ratio [OR], 8.08; 95% confidence interval [CI], 1.22–67.35; P = 0.008), but when the GCS score was 11 to 13, the factors associated with worst outcome were neuroworsening (OR, 3.43; 95% CI, 1.45–8.17; P = 0.002), seizures (OR, 7.94; 95% CI, 1.18–64.48; P = 0.02), and medical complications (OR, 4.24; 95% CI, 1.74–10.33; P = 0.0006). CONCLUSIONThere is a high percentage of surgery and worsening on computed tomographic scans in patients with moderate head injury. Neuroworsening, seizures, and medical complications as outcome predictors were more strongly associated with a GCS score of 11 to 13, whereas a low motor GCS score was more outcome-related in patients with GCS scores of 9 and 10.
Improved method for the simultaneous determination of d4T, 3TC and ddl intracellular phosphorylated anabolites in human peripheral-blood mononuclear cells using high-performance liquid chromatography/tandem mass spectrometry.

There is still a need for direct determination (i.e. without dephosphorylation) of nucleoside reverse transcriptase inhibitor (NRTI) triphosphorylated nucleotides in the peripheral-blood mononuclear cells (PBMCs) of HIV-positive patients. The objective of this paper was first to improve our previously described direct liquid chromatography/tandem mass spectrometry (LC/MS/MS) assay for stavudine triphosphate (d4T-TP). Preparation of PBMCs was modified to reduce degradation of d4T-TP during cell preparation and to simplify this step for routine use in clinical units. The performance of several HPLC columns was compared in order to improve the stability of peak shape over time. The SMT C(18) column was replaced by a Supelcogel ODP-50, thereby reducing two-fold the concentration of the first standard. Various internal standards were compared to optimize peak shape and remove an interfering peak in LC. 2-Chloroadenosine 5prime prime or minute-triphosphate was chosen as the most appropriate internal standard. Substitution of the narrowbore column by a microbore column (150 x 0.32 mm) is also presented and discussed. Secondly, this improved method was successfully applied to the simultaneous determination of d4T-TP, dideoxyadenosine triphosphate (ddA-TP) and lamivudine triphosphate (3TC-TP) in PBMCs, which is useful in view of the common use of NRTI combinations. The method was subsequently applied to clinical samples from HIV-positive patients receiving antiretroviral therapy containing d4T, ddl and/or 3TC. This method can be used simply and routinely on approximately 200 samples per week, using commercially available instruments and with a simple cell lysis as sample treatment.
Greedy Optimization Algorithm for the Power/Ground Network Design to Satisfy the Voltage Drop Constraint

With the process technological progress in recent years, low voltage power supplies have become quite predominant. With this, the voltage margin has decreased and therefore the power/ground design that satisfies the voltage drop constraint becomes more important. In addition, the reduction of the power/ground total wiring area and the number of layers will reduce manufacturing and designing costs. So, we propose an algorithm that satisfies the voltage drop constraint and at the same time, minimizes the power/ground total wiring area. The proposed algorithm uses the idea of a network algorithm [1] where the edge which has the most influence on voltage drop is found. Voltage drop is improved by changing the resistance of the edge. The proposed algorithm is efficient and effectively updates the edge with the greatest influence on the voltage drop. From experimental results, compared with the conventional algorithm, we confirmed that the total wiring area of the power/ground was reducible by about 1/3. Also, the experimental data shows that the proposed algorithm satisfies the voltage drop constraint in the data whereas the conventional algorithm cannot.
EM channel characteristics and their impact on MAC layer performance in underwater surveillance networks

Wireless communications and multihop networking based on electromagnetic EM radios have been considered as an alternative to acoustic communications in seawater because in typical applications for networked underwater sensing, EM waves are much less susceptible to multipath distortion and environmental noise. In this paper, we discuss the characteristics of EM channels in seawater and derive a novel EM signal propagation model. Based on the propagation model, we implement in the QualNet network simulator an EM underwater surveillance network for studying the impact of these unique characteristics of underwater EM channels on the media access control MAC layer performance. Both a single-hop network model and a multihop network model are simulated. Simulation results show that the carrier sense multiple access without or with acknowledgement CSMAWithoutACK or CSMAWithACK, respectively has advantages over ALOHA and multiple access with collision avoidance in terms of packet average delay, packet delivery ratio, and MAC scheme overhead. In the multihop network model, the use of CSMAWithoutACK significantly reduces the packet average delay and the MAC scheme overhead, and both CSMAWithoutACK and CSMAWithACK achieve more than 90% packet delivery ratio. Therefore, CSMAWithoutACK with no handshaking via request-to-send and clear-to-send control packets is the most appropriate MAC protocol to be used in multihop EM-based underwater surveillance networks. © Her Majesty the Queen in Right of Canada 2014. Reproduced with the permission of the Minister of Industry Canada
Acute lymphoblastic leukaemia: correlation between morphological/immunohistochemical and molecular biological findings in bone marrow biopsy specimens

Background—Although numerous antibodies suitable for use on paraffin wax embedded sections are available for the subtyping of acute leukaemia (acute myelogenous leukaemia (AML) and acute lymphoblastic leukaemia (ALL)) in bone marrow biopsy sections, unequivocal identification of the cell line involved is sometimes impossible. Methods—Forty eight formalin fixed, paraffin wax embedded bone marrow biopsy specimens that had been decalcified in EDTA were investigated, including 42 thought to exhibit ALL on the basis of bone marrow smears. Five specimens exhibited AML and one biphenotypic leukaemia, as diagnosed immunohistochemically in bone marrow biopsies. Immunostaining was performed with antibodies against relatively specific B and T cell antigens. The blasts were investigated for rearrangements of the immunoglobulin heavy chain (IgH) and the T cell antigen receptor (TCR) genes. Results—Amplifiable DNA was obtained from all 48 specimens. An IgH gene rearrangement was detected in 20 of 23 c-ALL specimens. Four of seven T cell ALL (T-ALL) specimens had a TCR-γ gene rearrangement, and the one B cell ALL (B-ALL) specimen exhibited a clonal IgH gene. Three of four cases of unclassifiable ALL could be assigned to the B cell lineage on the basis of gene rearrangement analysis. Seven cases originally diagnosed in smears as ALL were rediagnosed as AML (n = 5) or biphenotypic leukaemia (n = 2) because of immunohistochemical reactivity for myeloperoxidase or lysozyme. Two of these AML cases and two of three cases of biphenotypic leukaemia exhibited a monoclonal IgH gene rearrangement. Conclusions—Acute leukaemia can be subtyped in bone marrow sections with a limited panel of antibodies suitable for use on paraffin wax embedded sections (against CD3, CD10, CD20, CD79a, myeloperoxidase, and lysozyme). In patients with ALL and a diagnostically equivocal immunophenotype, gene rearrangement analysis might indicate whether the B or T cell lineage is involved.
Current knowledge on playing football in hot environments

Schulthess Klinik and F-MARC, FIFA Medical Assessment and Research Centre, Zurich,SwitzerlandCorresponding author: Justin Grantham, ASPETAR, Research and Education Centre, Qatar Orthopaedic and SportsMedicine Hospital, PO Box 29222, Doha, Qatar. Tel: 1974 4413 2570, Fax: 1974 4413 2034, E-mail: justin.grantham@aspetar.comAccepted for publication 22 July 2010
Early programming of adult diseases in resource poor countries

Considerable evidence now exists to suggest that early exposure to nutritional deprivation can have long term consequences to health, with low birth weight now considered a risk factor for later health outcomes such as coronary heart disease, stroke, type 2 diabetes, and the metabolic syndrome. Of importance, such effects are most exaggerated when faced with over-nutrition in later life, forming the basis for the “thrifty phenotype” hypothesis. The evidence in support of these associations comes largely from retrospective cohort studies in which adult outcomes were correlated with birth weight records. Relatively little data is available from developing countries, where long term record keeping of birth weight data has not been a high priority. Arguably however, such countries are at the greatest risk from the mismatch of early nutritional deprivation and later nutritional affluence. This paper explores the importance of the “developmental origins of health and disease” hypothesis in resource poor countries.
Deregulation of Electron Flow within Photosystem II in the Absence of the PsbJ Protein*

The photosystem II (PSII) complex of photosynthetic oxygen evolving membranes comprises a number of small proteins whose functions remain unknown. Here we report that the low molecular weight protein encoded by the psbJ gene is an intrinsic component of the PSII complex. Fluorescence kinetics, oxygen flash yield, and thermoluminescence measurements indicate that inactivation of the psbJ gene in Synechocystis6803 cells and tobacco chloroplasts lowers PSII-mediated oxygen evolution activity and increases the lifetime of the reduced primary acceptor Q A − (more than a 100-fold in the tobacco ΔpsbJ mutant). The decay of the oxidized S2,3 states of the oxygen-evolving complex is considerably accelerated, and the oscillations of the Q B − /S2,3 recombination with the number of exciting flashes are damped. Thus, PSII can be assembled in the absence of PsbJ. However, the forward electron flow from Q A − to plastoquinone and back electron flow to the oxidized Mn cluster of the donor side are deregulated in the absence of PsbJ, thereby affecting the efficiency of PSII electron flow following the charge separation process.