Miracle-dz/UniFAR-FTUs huggingface dataset:
Clarification of the roles of the telomere binding protein TPP1 and telomerase RNA in telomerase trafficking

Telomerase is recruited to telomeres during DNA replication to catalyze the extension of telomeres. The intranuclear mobilization of telomerase is critical for the activity of telomerase and the elongation of telomeres in human cells. Two proteins in the shelterin complex that are found at telomeres have been implicated in recruitmet of telomerase to telomeres. Here we used two distinct protein-tethering systems to clarify the individual roles of TPP1 and TIN2 in telomerase trafficking. We found that tethering the OB domain of TPP1 (known to mediate interacton with telomerase) to telomeres via TRF2 (independently of TIN2) resulted in recruitment of telomerase to telomeres. Expression of the TPP1-OB-TRF2 fusion protein also resulted in increased hTR-telomere association in cells depleted of TIN2. Using a second tethering system we also found that telomerase can be recruited to an internal site in chromosome 7 when TPP1 is tethered there via lacO-lacR interaction. Importantly, the accumulation of telomerase at this non-telomeric site is independent of TIN2. Together, our results suggest that TPP1, not TIN2, mediates the recruitment of telomerase to telomeres by the shelterin complex, and that the OB fold of TPP1 is sufficient for this process.
1 CONSUMER BEHAVIOUR ANALYSIS : II . CONSUMER BRAND CHOICE

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Embedding Small Business and Entrepreneurship Training within the Rural Context

Rural small business owners face challenges that are not generally present in urban locations. As a result, they need training programmes that are specific to this context. This paper presents a framework for such a programme. First, the programme is based on the perspective that rural business owners are strongly motivated, but not necessarily by financial goals alone. Second, the owners must approach the issue of business development with the realization that multiple income sources may be required for business sustainability. Third, the primary resources are the owners themselves, along with their family, their business and their community – all available locally. Finally, the curriculum is delivered using examples of successful rural entrepreneurs.
Job Satisfaction of Employees Undergoing Public Sector Reform in Fiji

This study analyses job satisfaction in the Maritime Safety Authority of Fiji (MSAF), a public sector entity undergoing reform. Results of this study determine that the level of MSAF employee job satisfaction by dimension was predominantly low; employees had a low level of intrinsic satisfaction, an average level of extrinsic satisfaction, and a low level of general satisfaction; and employees’ level of job satisfaction was significantly lower than the reference group. This research makes its theoretical contribution primarily to the literature on public service job satisfaction, and to the scarce theoretical strands relating to public service safety organisations. A practical outcome of this research is its contribution towards ongoing public sector reforms in Fiji in general and MSAF and the maritime industry in particular.
Repellency of Petroleum Ether Extract of Some Indigenous Plants Against Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)

Petroleum ether extracts of V. officianalis , P. harmala , S. lappa and A. indica oil were studied for their repellent effect against T. castaneum . Out of these plants, V. officianalis showed highest (80.83%) repellency during the first week at 1000 µg/cm 2 , which was significantly higher than 58.83% in A. indica oil (used as standard). Plant extracts showed highest repellency during the first week which significantly decreased in the second and fourth weeks and completely exhausted after the eighth week. Application rate of 1000 µg/cm 2 was the most effective followed by 500 and 250 µg/cm 2 . Petroleum ether extract of V. officianalis having 49.25 and 42.25% at 1000 and 500 µg/cm 2 and S. lappa having 47.75% average repellency were promising repellents against T. castaneum adults over eight weeks period, respectively, as against 56.63% in A. indica oil.
THE INFLUENCE OF STRAIN AND AGE ON SOME EGG QUALITY PARAMETERS OF COMMERCIAL LAYING HENS

Аbstract: The experiment was conducted to evaluate the influence of strain and age on some egg quality parameters (egg weight and egg shell strength) of commercial laying hens. Layers of lines ISA Brown and DeKalb White were examined in period of nine months. Both lines were at same age (28 weeks) and were confined in common facility in battery cages. Laying hens were reared and fed according to standard breeding technology. Once a month 25 eggs of each line were taken to be examined for eggshell strength and egg weight, or in other words, total 450 table eggs were tested. Analises were done in Laboratory for testing egg quality by gaudges Egg Multi Tester EMT 5200 and Egg shell Gauge (Robotmation Co. Ltd., Tokyo, Japan) which have computerised equipment to examine quality and physical characteristics of eggs. Statistic analyses on results were done by computer sub-programme ANOVA and Fisher's LSD test to determine the level of statistical significant difference between examined factors. Egg weight was under significant impact of age (P ≤ 0,05), but not under the influence of strain, although eggs of ISA Brown line were insignificantly heavier than eggs of DeKalb White line. Eggs were heaviest at layers with older age, while they were lightest at younger birds. The results have shown significant differences (P ≤ 0,05) in eggshell strength compared to line influence (genotype) and months of age. Eggs from ISA Brown laying hens had much better and eggshell strength than those eggs from DeKalb White. Correlations between eggshell strength and egg weight were with significant (Р < 0,05) negative value, which indicating that with increasing egg weight decreases of eggshell strength.
Wage Discrimination Measurement: In Defense of a Simple but Informative Statistical Tool

We examine several functional and numerical measures of wage discrimination in the spirit of the Lorenz curve. The First Order discrimination curve is based on the comparison of the wage CDF of two subpopulations. Three dierent Second Order discrimination curves are derived to re…ne the …rst one by taking into account inequality and/or e¢ ciency in the wage distribution across the two groups. We explore the relationship between these curves and some � Butler and McDonald (1987) developed about two decades ago the similar concept of interdistributional Lorenz curve and the main idea of our paper was already implicit in the work of some other scholars. However, our paper seems to constitute the …rst systematic study of these curves as a …rst step towards the derivation of partial dominance criteria in the analysis of discrimination data. y We thank Alain Trannoy for useful comments. The usual disclaimer applies. z Manufacture des Tabacs, Aile Jean-Jacques Laont 21, allee de Brienne, F-31000 Toulouse. E-mail: lebre-
On the assessment of aridity with changes in atmospheric CO2

A recent interpretation of climate model projections concluded that “warmer is more arid. ” In contrast, dust records and other evidence have led the geoscience community to conclude that “warmer is less arid” leading to an aridity paradox. The “warmer is more arid” interpretation is based on a projected increase in the vapour pressure deficit (∼ 7–9% K−1) that results in a projected increase in potential evaporation that greatly exceeds the projected increase in precipitation. However, the increase in potential evaporation does not result in an increase in (actual) evaporation which remains more or less constant in the model output. Projected changes in the long‐term aridity can be assessed by directly interrogating the climate model output. To that end, we equate lack of precipitation with meteorological aridity and lack of runoff with hydrologic aridity. A third perspective, agro‐ecological aridity, is not directly related to the water lost but rather to the carbon gain and is equated with the reduction in photosynthetic uptake of CO2. We reexamine the same climate model output and conclude that “warmer is less arid” from all perspectives and in agreement with the geological records. Future research will need to add the critical regional and seasonal perspectives to the aridity assessments described here.
Phase change with voids and bubbles

We investigate phase transition at the macroscopic level. The main novelty of our approach is the possibility of having voids during the phase change. This aspect is described in the model by the mass balance equation which results in a pressure which has a paramount importance. The state variables are the temperature, the strain tensor, and the volume fractions of the two phases (whose evolutions are described by a vectorial equation coming from the principle of virtual power and related to microscopic motion responsible for the phase change). The theory accounts for the main physical phenomenons, for instance, for the cavitation phenomenon and for pressure dependance of the phase change temperature. The scope of the predictive theory is illustrated with uni-dimensional examples.
Numerical Simulation of Recycled Concrete Using Convex Aggregate Model and Base Force Element Method

By using the Base Force Element Method (BFEM) on potential energy principle, a new numerical concrete model, random convex aggregate model, is presented in this paper to simulate the experiment under uniaxial compression for recycled aggregate concrete (RAC) which can also be referred to as recycled concrete. This model is considered as a heterogeneous composite which is composed of five mediums, including natural coarse aggregate, old mortar, new mortar, new interfacial transition zone (ITZ), and old ITZ. In order to simulate the damage processes of RAC, a curve damage model was adopted as the damage constitutive model and the strength theory of maximum tensile strain was used as the failure criterion in the BFEM on mesomechanics. The numerical results obtained in this paper which contained the uniaxial compressive strengths, size effects on strength, and damage processes of RAC are in agreement with experimental observations. The research works show that the random convex aggregate model and the BFEM with the curve damage model can be used for simulating the relationship between microstructure and mechanical properties of RAC.
A Review on Different Median based Filtering Techniques

This paper presents an evaluation on digital image filtering methods. The main objective is on median filtering and its improvements like hybrid median filtering, relaxed median filtering etc. It has been found that the still much research is required in median filtering demands certain sort of improvements as it is preeminent for salt and pepper noise only. The survey has shown the different short comings of earlier work.
Volunteering at the extensive margins in Developing Countries: Extrinsic or Intrinsic Motives?

The goal of this paper was to identify the reasons behind the supply for free of labor in a world where rationality requires people to avoid resource misallocation and to care mainly for their own welfare. We based the identification strategy on simple consumption and investment models with a focus on developing countries for the empirical part. Using some probability models and estimation techniques , we found an ambiguous coexistence of both intrinsic and extrinsic motives for volunteering.
Indian Ocean Rim Association for Regional Cooperation (IOR-ARC): Impact on Australia's Trade

Estimates of the gravity equation explaining trade flows between Australia and other member countries of IOR-ARC indicate that the signal of market-driven economic cooperation within IOR-ARC is strong due to the presence of APEC members. The intra-industry trade analysis between Australia and other IOR-ARC countries further confirms that the market-driven economic cooperation between them is feasible.
Effect of Polyzyme in Broilers Fed with Corn (Zea mays L.) Bran-Based Diets

One hundred and fifty broilers were randomly assigned to 5 dietary treatments of 3 replicates each. Five diets (Diet 1: basal, corn-based, with no Polyzyme enzyme; Diet 2: 20% corn bran with Polyzyme; Diet 3: 20% corn bran without Polyzyme; Diet 4: 40% corn bran with Polyzyme and Diet 5: 40% corn bran without Polyzyme) were formulated and offered to the birds. The aim was to compare the performance of the birds on the enzyme-supplemented diets and the un-supplemented diets with those on the corn-based diets. Feed intake and weight gain were significantly (P<0.05) higher in birds on the enzyme-supplemented diets compared to those on the basal diets or diets without enzyme supplementation. There was no significant difference in the apparent nutrient digestibility among the 5 dietary treatments. The results indicated that corn bran can be incorporated in broiler feed at 40% with supplementation with enzyme without any deleterious effect on the birds.
Matrix Resin Effects on Selected Physicochemical Properties of Amorphous Calcium Phosphate Composites

Amorphous calcium phosphate (ACP)-filled bioactive composites show promise in a number of dental applications. This study focused on polymer-filler bonding as a way to improve physicochemical properties of these composites. Photoactivated resins were formulated from ethoxylated bisphenol A dimethacrylate (EBPADMA), triethylene glycol dimethacrylate (TEGDMA), 2-hydroxyethyl methacrylate (HEMA) and methacryloxyethyl phthalate (MEP) with four different EBPADMA/TEGDMA mass ratios (1.0, 0.67, 0.5 and 0.25) and a constant HEMA/MEP mass ratio of 2.0. Composites were prepared with the zirconia-ACP (mass fraction 40%). Composites and copolymers were evaluated for their biaxial flexure strength (BFS), degree of vinyl conversion (DC) and water sorption (WS). Composites were also assessed for mineral ion release and polymerization shrinkage (PS). Independent of resin composition, the average BFS was 130±24MPa for both the dry and aqueous-immersed copolymers, but was 70±11MPa for the dry composites and 47±7MPa for the wet composites. The average DC of all of the composites was 84±5% and showed little dependence on resin composition. The WS of both copolymers and composites was 3.2±0.6% with resin composition being a factor in only one formulation EBPADMA/TEGDMA 0.67. Overall ion release was significantly above the theoretical minimum necessary for remineralization. However, Ca ion levels were lower than that observed previously for similar composites based on resins without MEP. This is most likely due to their binding with the carboxylic acid groups of MEP. Since the DC of the copolymers and the PS of the composites both increased with decreasing EBPADMA/TEGDMA ratio in the resin, elevating the EBPADMA/TEGDMA ratio in the resin seems to be an obvious way to improve the PS of composites without adversely affecting the BFS, WS and/or ion release, while only marginally reducing the DC.
SCDC: bulk gene expression deconvolution by multiple single-cell RNA sequencing references

Recent advances in single-cell RNA sequencing (scRNA-seq) enable characterization of transcriptomic profiles with single-cell resolution and circumvent averaging artifacts associated with traditional bulk RNA sequencing (RNA-seq) data. Here, we propose SCDC, a deconvolution method for bulk RNA-seq that leverages cell-type specific gene expression profiles from multiple scRNA-seq reference datasets. SCDC adopts an ENSEMBLE method to integrate deconvolution results from different scRNA-seq datasets that are produced in different laboratories and at different times, implicitly addressing the problem of batch-effect confounding. SCDC is benchmarked against existing methods using both in silico generated pseudo-bulk samples and experimentally mixed cell lines, whose known cell-type compositions serve as ground truths. We show that SCDC outperforms existing methods with improved accuracy of cell-type decomposition under both settings. To illustrate how the ENSEMBLE framework performs in complex tissues under different scenarios, we further apply our method to a human pancreatic islet dataset and a mouse mammary gland dataset. SCDC returns results that are more consistent with experimental designs and that reproduce more significant associations between cell-type proportions and measured phenotypes.
Simulating individually targeted transcranial electric stimulation for experimental application

Transcranial electric stimulation (tES) induces electric fields that are subject to a complex interaction with individual anatomical properties, such as the low-conducting human skull, the distribution of cerebrospinal fluid or the sulcal depth, as well as stimulation target location and orientation. This complex interaction might contribute to the heterogenous results that are commonly observed in applications of tES in humans. Targeted tES, on the other hand, might be able to account for some of these individual factors. In the present study, we used the finite-element method (FEM) and head models of twenty-one participants to evaluate the effect of individually targeted tES on simulated intracranial current densities. Head models were based on an automated segmentation algorithm to facilitate processing in experimental sample sizes. We compared a standard stimulation montage to two individually optimized tES montages using an Alternating Direction Method of Multipliers (ADMM) and a Constrained Maximum Intensity (CMI) approach. A right parietal target was defined with three different orientations. Individual current densities showed varying intensity and spatial extent near the lower limit at which physiological efficacy of electric fields can be assumed. Both individually optimized targeting algorithms were able to control the electric field properties, with respect to intensities and/or spatial extent of the electric fields. Still, across head models, intensity in the stimulation target was constrained by individual anatomical properties. Thus, our results underline the importance of targeted tES in enhancing the effectiveness of future tES applications and in elucidating the underlying mechanisms.
Cytotoxic effect of hydroalcoholic extract from Thymus daenesis Celak on MCF-7 cancer cells line

Cancer is one of the non-communicable chronic diseases which involves broad group of diseases. Like other chronic diseases, this disease is occurred in any person, age groups and all races and is considered as a major hygiene problem which is effective on the heath of society. Cancer is the second most common cause of death after cardiovascular diseases and accidents in less developed countries (Siegel et al., 2011). Breast cancer is the second most common cancer after lung cancer and the most common type of cancer among women around the world (Hamta & Parvini, 2011). This cancer is responsible for 33 percent of all cancers in women and 20 percent of deaths from cancer, incidence of breast cancer is increasing in developing countries and in many parts of the world, it Journal of Herbal Drug
Acute Toxicity of Xylene on the African Catfish Clarias gariepinus

Acute toxicity of xylene on an African catfish ( Clarias gariepinus ) was carried out. A total of 210 catfish C. gariepinus juveniles with mean length 15.20±2.3cm, and mean weight of 10.23±2.6g were obtained from the University of Port Harcourt Demonstration Farm. The test was determined for 96hour median lethal concentration using concentrations of 250ml/l, 200ml/l, 150ml/l, 100ml/l, 50ml/l, 25ml/l and 0.0ml/l (control) which gave a LC 50 value of 63.965ml/l with upper and lower confidence limits at 106.53 ml/l and 37.82ml/l respectively. The median lethal time LT 50 recorded was 55.7 hours. There was a strong correlation between (%) mortality in Probits and the Log 10 Dose (R²=0.9772). There was statistical significance (P>0.05) in the number of mortality observed in the six concentrations from 24 hours to 96 hours of exposure and high percentage mortalities were recorded as the concentration of the toxicant increased. No mortalities were recorded in the control. The cumulative mortality recorded after exposure of C. gariepinus to xylene was time dependent. Based on this the high percentage mortalities of the fish species it is therefore recommended that the use of this chemical be minimized and proper contingency plans be carried out before discharging this toxicants into the aquatic environment. Waste from this chemical and spill incidences should be detoxified to a less toxic level before disposing into the aquatic environment. The results obtained may provide valuable information for formulation of environmental policies and serve as a model for bio-monitoring of the aquatic environment. Keywords : Acute toxicity, Clarias gariepinus , and Xylene
HIV/AIDS Prevention Knowledge among Youth in Cape Town, South Africa

HIV/AIDS knowledge is an important component of HIV/AIDS risk prevention strategies that may influence engagement in high risk behavior. This paper examines HIV/AIDS prevention knowledge among a representative sample of 4,174 youth living in Cape Town, South Africa. Data come from the Cape Area Panel Study (CAPS), and include black, coloured, and white respondents ages 14-22. Using an open-ended question, respondents were asked to name ways people can protect themselves from HIV/AIDS infection. Nearly everyone could name at least one method of preventing HIV infection, and respondents named two methods on average. Condoms, abstinence, and limiting the number of sexual partners/having only one sexual partner were the most frequently named prevention methods. Multivariate analysis was used to analyze correlates of specific forms of HIV/AIDS prevention, as well as the total number of prevention methods named by each respondent. Having had sex, highest grade completed, and race were the most commonly significant correlates across models. Race interaction terms were also significant, suggesting that the significance of HIV/AIDS knowledge correlates varies across racial groups. Overall, the results suggest that more depth of knowledge about HIV/AIDS is needed among South African youth to ensure proper protection from the disease, and that HIV/AIDS education might be more successful if tailored to specific racial/ethnic groups.