Miracle-dz/UniFAR-FTUs huggingface dataset:
The intracisternal A-particle proximal enhancer-binding protein activates transcription and is identical to the RNA- and DNA-binding protein p54nrb/NonO

The long terminal repeats of murine intracisternal A particles (IAPs) contain an IAP proximal enhancer (IPE) element that is inactive in murine F9 embryonal carcinoma cells and active in the parietal endoderm cell line PYS-2. The element binds efficiently to a 60-kDa IPE-binding protein (IPEB) present in PYS-2 cells but poorly to F9 proteins, suggesting a role for IPEB in regulating IAP expression. We have purified calf thymus IPEB, which binds to the IPE and transactivates a reporter gene in HeLa cell extracts. Based on the peptide sequence of the purified calf IPEB, we have cloned a 420-bp cDNA and showed that the encoded protein is the homolog of human p54nrb and mouse NonO, which are characterized by the presence of two RNA recognition motifs. We show that p54nrb is an IPE-binding transcription activator with its DNA-binding and activation domains in the N- and C-terminal halves, respectively. The activation domain of p54nrb is active in HeLa, PYS-2, and F9 cells, whereas p54nrb as a whole molecule is active in HeLa and PYS-2 cells but not in F9 cells. Thus, the lack of activity of p54nrb in F9 cells is due to an ineffective DNA-binding domain. We demonstrate that p54nrb also binds to a pre-mRNA. Based on the close sequence relatedness of this protein to PSF, which is required for pre-mRNA splicing in vitro, we discuss the possibility that p54nrb has dual roles in transcription and splicing.
ABCC1, an ATP Binding Cassette Protein from Grape Berry, Transports Anthocyanidin 3-O-Glucosides[W][OA]

This work provides biochemical evidence that ABCC transporters are directly involved in anthocyanin transport into plant vacuoles. The presence of reduced glutathione is a prerequisite for the transport. Our data support that anthocyanins and glutathione are cotransported but that no glutathione anthocyanin conjugate is formed. Accumulation of anthocyanins in the exocarp of red grapevine (Vitis vinifera) cultivars is one of several events that characterize the onset of grape berry ripening (véraison). Despite our thorough understanding of anthocyanin biosynthesis and regulation, little is known about the molecular aspects of their transport. The participation of ATP binding cassette (ABC) proteins in vacuolar anthocyanin transport has long been a matter of debate. Here, we present biochemical evidence that an ABC protein, ABCC1, localizes to the tonoplast and is involved in the transport of glucosylated anthocyanidins. ABCC1 is expressed in the exocarp throughout berry development and ripening, with a significant increase at véraison (i.e., the onset of ripening). Transport experiments using microsomes isolated from ABCC1-expressing yeast cells showed that ABCC1 transports malvidin 3-O-glucoside. The transport strictly depends on the presence of GSH, which is cotransported with the anthocyanins and is sensitive to inhibitors of ABC proteins. By exposing anthocyanin-producing grapevine root cultures to buthionine sulphoximine, which reduced GSH levels, a decrease in anthocyanin concentration is observed. In conclusion, we provide evidence that ABCC1 acts as an anthocyanin transporter that depends on GSH without the formation of an anthocyanin-GSH conjugate.
Incidence of the mucopolysaccharidoses in Taiwan, 1984–2004

Previous studies on the incidence of the various types of mucopolysaccharidoses (MPS) in different populations have shown considerable variation. However, information regarding the incidence of MPS in the Asian population is lacking. An epidemiological study of the MPS disorders in Taiwan using multiple ascertainment sources was undertaken, and incidences of different types of MPS during the period of 1984–2004 were estimated. We compared our data with previous reports in different populations. The combined birth incidence for all MPS cases was 2.04 per 100,000 live births. MPS II (Hunter syndrome) had the highest calculated birth incidence of 1.07 per 100,000 live births (2.05 per 100,000 male live births), comprising 52% of all MPS cases diagnosed. The birth incidences of MPS I (Hurler syndrome), III (Sanfilippo syndrome), IV (Morquio syndrome), and VI (Maroteaux‐Lamy syndrome) were 0.11, 0.39, 0.33, and 0.14 per 100,000 live births, respectively, which accounted for 6%, 19%, 16%, and 7% of all MPS, respectively. No cases of MPS III D (Sanfilippo syndrome type D), MPS IV B (Morquio syndrome type B), MPS VII (Sly syndrome) or MPS IX were ascertained during the study period. Overall incidence of MPS in Taiwan was consistent with that reported in Western populations. However, in contrast to the higher incidence of MPS I in most Western populations, this study showed a higher incidence of MPS II in Taiwan. It remains to be investigated whether this discrepancy is attributed to the under‐diagnosis of MPS I in Taiwan or to ethnic differences. © 2009 Wiley‐Liss, Inc.
The effects of laterotactile information on lump localization through a teletaction system

The human finger pad is known to be highly sensitive to lateral skin deformation, which is present during the palpation of soft objects containing hard lumps. This study investigates the effects of this lateral information on the accuracy and reliability of lump localization through a teletaction system. The results are expected to benefit future telesurgical robots for remote palpation. A previously proven deformation-based tactile feedback system was modified to include laterotactile feedback using a new version of a biologically-inspired fingertip sensor (TACTIP) and a remotely-actuated electromechanical shape display. These novel devices were mounted on to a newly developed teleoperated system equipped with visual and force feedback, through which test subjects palpated a number of soft artificial tissue samples. The level of lateral feedback was altered between trials, with constant visual, force, and normal-direction tactile feedback provided throughout. The surprising results show that the addition of lateral feedback offered no benefit to the subjects' ability to detect and localise embedded objects. Reasons for this observation are discussed. It is concluded that normal-direction tactile feedback is likely to be sufficient for lump detection even when lateral motions are made, although feedback from the subjects indicates that the addition benefited their perception of interactions with the system.
Real exchange rate and economic complexity in a North-South structuralist BoPG model

The main objective of this work is to analyze theoretically and empirically the interrelationships between the real exchange rate (RER) and the economic complexity level in a Keynesian-Structuralist framework for two regions, the (developed) North and the (developing) South. In the analysis presented here, the RER level matters for influencing the industrial (manufacturing) share of GDP as well as the GDP growth rate compatible with the balance-of-payments equilibrium. Higher levels of economic complexity influence the South growth rate, depending on the effects of innovation, knowledge stock, and human capital on international trade. The empirical evidence in panel data suggests that undervalued RER and a higher manufacturing share in the developing-countries sample exhibit positive and significant effects on the economic complexity level. JEL codes : O10, O33, L16 Keywords : manufacturing, technological frontier, real exchange rate
Positional cloning of silkworm white egg 2 (w‐2) locus shows functional conservation and diversification of ABC transporters for pigmentation in insects

The white, scarlet and brown genes of Drosophila melanogaster encode three half‐type ATP‐binding cassette (ABC) transporters. In Drosophila, precursors of ommochromes and pteridines are transported by White/Scarlet and White/Brown heterodimers, respectively. The white egg 2 (w‐2) mutant of the silkworm, Bombyx mori, has white eggs and eyes because of lack of ommochrome granules in the serosa and eyes. Here, we report that the silkworm w‐2 locus encodes an ortholog of Drosophila scarlet. Our results indicate that Bombyx Scarlet forms a heterodimer with Bombyx White to transport ommochrome precursors, suggesting that formation of a White/Scarlet heterodimer and its involvement in the transport of ommochrome precursors are evolutionarily ancient and widely conserved traits in insects. Contrary to dipteran insects, white and scarlet were juxtaposed in a head‐to‐tail orientation in the silkworm genome, suggesting that the origin of white and scarlet was a tandem duplication of their ancestral transporter gene. In Bombyx, White is also essential for the transport of uric acid in larval epidermis. However, our results suggest that a Bombyx White/Scarlet heterodimer is not involved in this process. Our results emphasize the functional conservation and diversification of half‐type ABC transporter families in insects, which may contribute to their extremely diverse color patterns.
Knowledge management: an integrative approach literature review

This paper is primarily a review and analysis of the literature associated with the field of knowledge management. In particular, it focuses on investigating how authors are measuring and addressing the implementation of KM in organisations. Existing KM models lack the integrative viewpoints. This paper is covering this gap by providing the integrative approach. That focuses on the integration of two essential approaches; KM as process and KM as infrastructure. A total of 250 papers published in peer-reviewed journals are carefully reviewed. Based on the content analysis the theoretical, and empirical antecedents in the field of KM had been explored. By integrating insights drawn from the previous studies as well as adding some new factors, the author proposed an integrative approach for the comprehensive understanding of KM. For academics, the proposed model provides a guide for future applied research and offers a critical review of significant knowledge measurement studies.
ScholarBase: Towards a Cross-Domain Knowledgebase for Linked Scholarly Data

Linked Data has gained a significant momentum over the last years through various initiatives embraced by academia and industry as well. In this paper, we introduce ScholarBase, a research project in progress that is aimed to serve as a Linked Data repository for cross-domain scholarly data. ScholarBase can be conceived as a knowledgebase that weaves links among scholars, institutions, research areas, publications, and geographical locations in a Linked Data fashion. Initially, the primary source of data stems from Google Scholar, specifically the profile pages of scholars. Subsequently, the collected dataset is transformed into a semantic-based format (i.e. RDF). The semantified dataset will enable a machine-readable expression of entity relationships, and can in turn be linked to external knowledge bases (e.g. DBpedia). Through the paper, we discuss the architecture and implementation of the project. ScholarBase can play an important role in the Linked Data web by opening extended opportunities for interesting applications, and data exploration of scholarly data.
Prophylaxis against wound infection following herniorrhaphy or breast surgery.

The effect of perioperative antibiotic prophylaxis on definite wound infections was assessed for 3202 herniorrhaphies or selected breast surgery procedures. Patients were identified preoperatively and monitored for greater than or equal to 4 weeks. Thirty-four percent of patients (1077/3202) received prophylaxis at the discretion of the surgeon; 86 definite wound infections (2.7%) were identified. Prophylaxis recipients were at higher risk for infection, with a higher proportion of mastectomies, longer procedures, and other factors. Patients who received prophylaxis experienced 41% fewer definite wound infections (odds ratio [OR], 0.59; 95% confidence interval [CI], 0.35-0.99; P = .04) and 65% fewer definite wound infections requiring parenteral antibiotic therapy (OR, 0.35; 95% CI, 0.15-0.88; P = .02) after adjustment for duration of surgery and type of procedure. Additional adjustment for age, body mass index, the presence of drains, diabetes, and exposure to corticosteroids did not change the magnitude of this effect meaningfully. The effect of prophylaxis was similar for all procedures studied. In the absence of formal guidelines, surgeons at these institutions administered prophylaxis preferentially to patients at highest risk.
Re-setting the biologic rationale for thermal therapy

This review takes a retrospective look at how hyperthermia biology, as defined from studies emerging from the late 1970s and into the 1980s, mis-directed the clinical field of hyperthermia, by placing too much emphasis on the necessity of killing cells with hyperthermia in order to define success. The requirement that cell killing be achieved led to sub-optimal hyperthermia fractionation goals for combinations with radiotherapy, inappropriate sequencing between radiation and hyperthermia and goals for hyperthermia equipment performance that were neither achievable nor necessary. The review then considers the importance of the biologic effects of hyperthermia that occur in the temperature range that lies between that necessary to kill substantial proportions of cells and normothermia (e.g. 39–42°C for 1 h). The effects that occur in this temperature range are compelling including—inhibition of radiation-induced damage repair, changes in perfusion, re-oxygenation, effects on macromolecular and nanoparticle delivery, induction of the heat shock response and immunological stimulation, all of which can be exploited to improve tumour response to radiation and chemotherapy. This new knowledge about the biology of hyperthermia compels one to continue to move the field forward, but with thermal goals that are eminently achievable and tolerable by patients. The fact that lower temperatures are incorporated into thermal goals does not lessen the need for non-invasive thermometry or more sophisticated hyperthermia delivery systems, however. If anything, it further compels one to move the field forward on an integrated biological, engineering and clinical level.
Does financial and agriculture sector development reduce unemployment rates? Evidence from Southern African countries

The paper examines empirically the impacts of agricultural sector value added and financial development on unemployment, using yearly data from 1995–2015. Eleven developing Southern African Development Community countries were selected for the study. The empirical analysis was carried out using second-generation econometric methods. The regression results revealed that both agricultural value added and financial development are important determinants of unemployment within the region. The results specifically show that agricultural value added is negatively associated with unemployment in both the short and long-run, although the long-run effect is many times bigger than the short-run impact. The results also show that in the long-run, both financial depth and financial efficiency are negatively associated with unemployment. Interactions between agricultural value added financial development and unemployment were further tested via panel bootstrap causality tests. The causality test results revealed the existence of significant one-way causality from agricultural value added to unemployment and from financial depth to unemployment for the region. It also showed that causality varies across individual countries within the region with different conditions, indicating the heterogeneous nature of the countries that make up the regional bloc.
Industrial Relations: Worker Codetermination and Collective Wage Bargaining

Trade unions and employers’ associations, collective bargaining, and employee representation at the workplace are the cornerstones of industrial relations systems in many developed countries. Germany stands out as a country with powerful works councils and a high coverage rate of collective bargaining agreements, supported by encompassing interest groups of employees and employers and by the state. The German case and the perceived stability of its industrial relations regime have attracted considerable attention among researchers and politicians, which also has to do with the country’s high productivity, comparably few strikes, and relatively minor employment problems. However, in recent years industrial relations in many countries have come under pressure. Increasing global competition, technological, demographic and organisational change, new forms of work, and other developments have challenged the traditional systems of industrial relations. Union membership and density have fallen in most developed countries (Schnabel 2013), whereas employers’ associations have remained relatively stable (OECD 2017). Across OECD countries, the share of workers covered by a collective agreement has shrunk from 45 percent in 1985 to 33 percent in 2015 (OECD 2017: 126). What is more, in the EU more than one third of employees has no formal representation at the workplace in the form of a trade union, works council or similar committee and/or a health and safety committee (Eurofound 2016: 74). At the same time, industrial relations systems have tried to adapt and become more flexible, and there has been some kind of decentralization in many countries.
For Sustainable Benefits and Legacies of Mega-Events: A Case Study of the 2018 PyeongChang Winter Olympics from the Perspective of the Volunteer Co-Creators

This study examined the outcome of the 2018 PyeongChang Winter Olympics from multiple perspectives. Tourism and sport researchers have focused on the legacy of mega-events, due to the opportunity they provide to transform a city positively, including through the development of the tourism industry. However, outcomes are not always positive, and the effects differ for every event. The effects of an event can be short-term or long-term. An event, to be truly successful, should aim to have a long-lasting and sustainable positive effect for everyone involved in hosting the event. Additionally, the outcome effects are perceived to be different by each stakeholder of any event. While the roles of volunteers are critical to the success of an event, their perspectives of the event outcomes have been relatively overlooked. Therefore, this study examined the legacy of the 2018 Winter Olympics, as the most recent and unique Olympics (also known as the Peace Olympics), from the perspectives of the volunteers as co-creators. Specifically, volunteers were asked to explain their perceptions of the success of the Olympics. Furthermore, they were asked to identify the legacy of volunteering and the legacy of the Olympics, in order to examine whether volunteers can distinguish the difference between the legacies of volunteering and the Olympics (i.e., event legacy). Using a modified version of content analysis, the results of the volunteer interviews indicated that volunteers identified the legacies of volunteering on a more personal level, emphasizing personal experience, while they identified the legacies of the Olympics on a community and national level.
Opinion mining and opinion spam: A literature review focusing on product reviews

Sharing the opinion is a very common task in the World Wide Web. They express their feelings in social networks, blogs forums and so on. They can write their reviews about products that they have purchased. These reviews are used by potential users to decide whether to buy the product or select another one. They are also used by product manufacturers to identify product popularity, estimate brand reputation and to find competitive information about their competitors. So it is not very strange that spammers target this area and post spam reviews beneath the products to achieve their goals. This kind of spam is called product review spam. This papers aims to represent a literature review of what have done on review spam recognition.
Alexithymia and Empathy Predict Changes in Autonomic Arousal During Affective Stimulation

Background:Alexithymia, the inability to describe one’s own emotions, is linked to deficits in empathy, manifesting as a diminished capacity to recognize or understand the emotions and mental states of others. Several brain centers of autonomic control and interoception that are activated in empathy are thought to misfunction in alexithymia. We hypothesized that individual differences in autonomic changes under affective stimulation might be associated with differences in alexithymia and empathy. Methods:We studied 21 healthy volunteers, comparing their alexithymia and empathy scores with changes in their sympathetic autonomic arousal, indexed by the palmar skin potential level, during 3 tasks: playing a computer game, performing mental arithmetic, and watching a negative emotional valence video. Results:Both autonomic and subjective sense of arousal increased at the beginning of each task and then gradually subsided over the course of the task. Higher autonomic arousal at the onset of the computer game was associated with higher empathy scores, and at the onset of the negative video with higher scores for both empathy and alexithymia. Alexithymia delayed the habituation of autonomic arousal during the computer game, while the empathy score was related to a faster decline in arousal during the negative video task. Conclusions:High alexithymia and high empathy scores were linked to increased autonomic arousal at the onset of emotional stimulation, but were distinguishable in the rates of habituation of the evoked arousal. Our data provide insight into the relationships among interacting psychological traits, physiologic regulation, and the arousal dimension of emotional experience.
Molecular Background of Estrogen Receptor Gene Expression in Endometriotic Cells

The molecular background of estrogen receptor (ER) expression is important to understand the pathophysiology of the high estrogen environment in endometriosis. However, the molecular details have not been fully understood. The objective of this study is to evaluate the molecular background of ERα and ERβ messenger RNA (mRNA) expression in endometriotic cells. The following summarizes our observations: (1) ERα mRNA expression in endometriotic cells was estimated to be approximately one-tenth of that in endometrial cells. (2) Three mRNAs, which include 3 different 5′-untranslated exons tagged to an open reading frame of wild-type ERα, were detected. (3) Expression of ERβ mRNA depends mostly on 0N promoter and includes 2 open reading frames: one for a wild-type ERβ1 and another for a splice variant ERβ2. (4) Expression of ERβ1 mRNA was approximately 40-fold higher than that in endometrial cells. (5) Expression of ERβ2 mRNA was almost at a comparable level of the ERβ1. 9 (6) ERα and ERβ mRNAs are equivalently expressed in endometriotic cells. These observations show the molecular background of ER mRNA expression in endometriotic cells and provide a clue to further understanding the estrogen-dependent pathophysiology leading to clinical application in endometriosis.