Miracle-dz/UniFAR-FTUs huggingface dataset:
Composition of lipopolysaccharides from Myxococcus fulvus and other fruiting and non-fruiting myxobacteria.

From vegetative cells of Myxococcus fulvus the lipopolysaccharide was isolated by the phenolwater procedure and its composition studied. Mannose, d-galactose, d-glucose, rhamnose, glucos-amine and galactosamine were found to be the main sugar constituents. Smaller amounts of ribose, xylose, arabinose, 3-O-methyl-d-xylose and 2-keto-3-deoxyoetonate were also found. Heptose was absent. Among the fatty acids iso-branched fatty acids were predominant, i.e. 13-methyl-tetradecanoic acid, 3-hydroxy-13-methyltetradecanoic acid, and 3-hydroxy-15-methyl-hexadecanoic acid. In addition non-branched fatty acids were present in small amounts, like dode-canoic, tetradecanoic, hexadecanoic and octadecanoic acid as well as 3-hydroxy-tetradecanoic and 3-hydroxy-hexadecanoic acid. The 2,3- and 3,4-unsaturated fatty acids detected in hydroly-sates were shown to be artifacts formed from 3-hydroxy fatty acids during hydrolysis. By gentle acidic treatment of the lipopolysaccharide lipid A was obtained containing glucosamine, fatty acids and phosphate as the main constituents. Ester-bound and amide-bound fatty acids were differentiated. Similar sugar and fatty acid compositions were found in the lipopolysaccharides of 5 fruiting and non-fruiting strains of myxobacteria. However, heptose which was absent in the other strains occurred in the lipopolysaccharide of Flexibacter Sp Fx 1/2.
Fast ARMA modelling of power spectral density functions

A method for real-time estimation of ARMA models from power spectral density functions is proposed. It is based upon the combination of LPC methods to estimate both numerator and denominator polynomial of the transfer function and an iterative scheme to attribute the appropriate parts of the power spectral density function to pole or zero modelling of the transfer function. This approach yields an estimate to the data which approximately corresponds to a quadratic fit on a logarithmic scale. As further distinctive features we mention that the estimation is fast compared to more traditional methods and that it allows for frequency-warped modelling.
Phytochemical fingerprinting of vegetable Brassica oleracea and Brassica napus by simultaneous identification of glucosinolates and phenolics.

INTRODUCTION Brassica vegetables have been related to the prevention of cancer and degenerative diseases, owing to their glucosinolate and phenolic content. OBJECTIVE Identification of glucosinolates, flavonoids and hydroxycinnamic acids in representative varieties of kale, cabbage and leaf rape. METHODOLOGY One local variety of each crop was evaluated in this study using a multi-purpose chromatographic method that simultaneously separates glucosinolates and phenolics. Chromatograms were recorded at 330 nm for flavonoid glycosides and acylated derivatives and 227 nm for glucosinolates. RESULTS Eight glucosinolates were identified in kale and cabbage, which exhibited the same glucosinolate profile, and 11 glucosinolates were identified in leaf rape. Furthermore, 20 flavonoids and 10 hydroxycinnamic acids were detected in kale and cabbage, while 17 flavonoids and eight hydroxycinnamic acids were found in leaf rape. CONCLUSIONS This study has provided a deeper and comprehensive identification of health-promoting compounds in kale, cabbage and leaf rape, thus showing that they are a good source of glucosinolates and phenolic antioxidants.
Conception et mise en oeuvre d'un système de base de connaissances pour l'ordonnancement dynamique de gestion de priorités, basé sur l'approche algorithmique

Ce rapport presente un modele de systeme de base de connaissances d'ordonnancement dynamique pour l'optimisation de la production dans un environnement manufacturier de plusieurs produits. L'objectif de ce travail est de fournir a l'utilisateur un outil d'aide a la decision dans le choix de calendriers de production realisables et optimaux. Nous avons presente une recherche bibliographique pour essayer de donner un apercu sur ce qui se fait aujourd'hui dans ce domaine en plein expansion et un passage en revue de possibilites existantes. La recherche bibliographique aborde la complexite d'un systeme manufacturier "job shop". Nous presentons une classification des problemes d'ordonnancement et les algorithmes utilises pour la resolution de ces problemes en fonction des criteres economiques a optimiser. Des exemples ont ete preesentes pour faciliter la comprehension. L'arrivee de la nouvelle technologie et son utilisation pour l'automatisation des systemes de production a suscite un grand interet dans le developpement des systemes d'ordonnancement dynamique en temps reel. Comme le systeme de production change de statut imprevisiblement, alors le systeme de base de connaissance pour l'ordonnancement dynamique que nous proposons sera une solution pour la resolution des problemes d'ordonnancement complexes. L'approche que nous proposons pour la construction de ce systeme est une combinaison des heuristiques et des regles de base, afin de pourvoir l'utilisateur de conseils et d'avis sur les decisions de gestion des priorites sur les lignes de production. Cet outil permet aussi a ce dernier de maitriser la situation du changement dynamique de l'environnement manufacturier. En outre, les informations generees sont utilisees pour le controle de la performance du systeme de production. La methodologie que nous avons utilisee, est assez differente de ce qui se fait a ce jour dans l'ordonnancement des systemes de production; une tentative de montrer une voie de recherche qui pourrait constituer une alternative interessante dans l'avenir.
Coping with career boundaries and boundary-crossing in the graduate labour market

Purpose – The purpose of this paper is to explore the nature and role of career boundaries for enabling/constraining career self-management (CSM) for occupational boundary-crossing in the UK graduate labour market (GLM). Design/methodology/approach – The data are provided by career history interviews with 36 UK graduates. The analysis contrasts transitions for those who started careers in low-, intermediate-, and high-skilled segments of the labour market. Findings – Availability of development and progression opportunities were the most prominent career boundary experienced. Ease of boundary-crossing differed by career stage and educational background. Boundaries enabled CSM by acting as psychological/external push factors, but push factors only aided progression to high-skilled segments for a third of graduates who started careers in underemployment. For the rest, an adaptation of expectations to labour market realities was observed. Research limitations/implications – Although career history interviews.
CSCW—WCSC: computer-supported cooperative work—what changes for the science of computing

Computer-supported cooperative work environments change some of the underlying beliefs about solutions that have been built for distributed computing. Electronic mail and file transfers have worked efficiently and effectively by breaking the information to be transferred into packets and reassembling the packets at the destination. This is not a viable solution for handling shared real time drawing or writing. Integrity has been maintained by locking out portions of a database until an update is completed. Such lockout is not always suitable in a groupware interface. Other solutions are necessary for resolving conflicts. Client-server architectures have worked well for managing distributed processing but replicated architectures. coupled with their fragility and synchronization problems are needed for groupware products in order to preserve acceptable local response time. The addition of multi-media to the environment complicates the problem more. Control of analog video requires a client-server environment which can build in intolerable delays as distances between communicating parties increase. This paper approaches design criteria for shared software from the human side and points out profound architectural problems that need to be solved if multi-media cooperative work environments are to function effectively.
Tumor necrosis factor-alpha activates pulmonary artery endothelial protein kinase C.

We investigated the hypothesis that tumor necrosis factor-alpha (TNF) activates pulmonary endothelial protein kinase C (PKC). Confluent bovine pulmonary artery endothelial monolayers were exposed to recombinant human TNF, and the translocation of PKC, an indicator of enzyme activation, was studied using both slot immunoblotting and immunofluorescence. For slot immunoblot analysis, membrane and cytosol lysate fractions were prepared, and PKC antigen was assessed using MC5 monoclonal anti-PKC antibody. TNF (1,000 U/ml for 15 min) induced translocation of PKC into the membrane. Immunofluorescence analysis with the MC5 antibody was also used. Monolayers treated with culture medium showed diffuse cytoplasmic fluorescence. In contrast, treatment with either TNF (1,000 U/ml for 15 min) or 1,2-dioctanoylglycerol (4 x 10(-5) M for 5 min), a diacylglycerol that activates PKC, resulted in translocation of fluorescence to the cell periphery; fine, punctate PKC-associated fluorescence was localized to the margins of cells. The TNF-induced translocation of PKC was inhibited using either IP-300 polyclonal anti-TNF antibody (indicating that the TNF effect was not due to the vehicle or contaminating endotoxin) or calphostin C (10(-6) M for 15 min), which inhibits PKC activation by interacting with the regulatory diacylglycerol-binding domain. TNF treatment had no effect on either the content of PKC, or of total protein, in the membrane + cytosol, and cycloheximide (40 microM for 5 min) did not alter the translocation of PKC induced by TNF; these results indicate that the effect of TNF on PKC translocation was related to neither de novo membrane synthesis of PKC (as opposed to translocation per se) nor nonspecific augmentation of protein synthesis. (ABSTRACT TRUNCATED AT 250 WORDS)
Gate driver design considerations for silicon carbide MOSFETs including series connected devices

In this study, design considerations of gate driver for silicon carbide (SiC) power devices is discussed. The work is focused in minimizing the common-mode current injection into the control circuit, thereby adapting the gate circuit to operate at higher dv/dt of fast switching transients. By reducing the common-mode interference with the control circuit, the signal integrity can be increased, spurious faults in the converter can be minimized and the reliability of the converter operation can be enhanced. The effect of the coupling capacitance of the isolation transformer in the gate driver design is taken into account. The shoot-through protection of the device is ensured based on device voltage measurement. The operation of the designed gate driver is validated through double pulse switching as well as continuous operation of various converters. All the corresponding test results are reported.
dx2-y2 symmetry and the pairing mechanism.

An important question is if the gap in the high temperature cuprates has d_{x^2-y^2} symmetry, what does that tell us about the underlying interaction responsible for pairing. Here we explore this by determining how three different types of electron-phonon interactions affect the d_{x^2-y^2} pairing found within an RPA treatment of the 2D Hubbard model. These results imply that interactions which become more positive as the momentum transfer increases favor d_{x^2-y^2} pairing in a nearly half-filled band.
Advanced Models for Target Validation & Drug Discovery in Prostate Cancer

Prostate cancer (PrCa) is one of the most prevalent malignant diseases among men in Western countries. Despite good initial treatment response is observed in the vast majority of PrCa patients, tumor relapse is observed in about 7-10% of patients undergoing standard antihormonal therapies with anti-androgens and/or GnRH antagonists. There is currently no cure for castration-resistant prostate cancer (CRPC), and median survival for these patients is only about 18 months. The high mortality rate of advanced cases is closely associated with invasive carcinomas and systemic metastasis, most frequently to the bone; and CRPC is almost always coincident with overexpression/amplification of the androgen receptor (AR) gene. CRPCs fail to respond to all currently prescribed first line anti-hormones such as flutamide, nilutamide or casodex (bicalutamide). The second line anti-tumor treatment against CRPC, frequently taxanes like docetaxel, is administered to patients after the first or second relapse. Taxanes are often supported by zoledronic acid targeting bone metastases. These drugs and drug combinations are in most cases initially effective, but rarely curative. Although taxanes do show potent anti-tumor effects, advanced PrCa patients develop resistance and only gain several months of survival time. For decades, no major improvements have been made in the therapy of advanced PrCa. However, this situation has dramatically changed over the last few years with a number of novel, promising drug concepts in the pipeline, some of which have already entered the market. It is not expected that these drugs will be curative, and relapses and therapy failures are expected to develop in these cases which may remain fatal. Therefore, the demands will remain high to develop yet better drugs, and more faithful models that reliably mimic at least some of the key aspects of advanced prostate cancers. These models are required to recapitulate the mechanisms leading to therapeutic resistance & failure also for new compounds and drug combinations entering the clinics now. There will remain a need to mimic which mechanisms either in the tumor cells or in the tumor-associated microenvironment may have contributed to the resistance. This consistent high demand in improved models will go hand in hand with a better understanding of the pros and cons of the various models available. Continued development of improved model systems for PrCa and in particular CRPC could lead to an informed ranking according to the maximal throughput in drug screens that can be achieved, balanced against their true informative value and relevance for recapitulating clinical PrCa. A systematic side-by-side comparison of available models, e.g. cell lines, organotypic three-dimensional cultures and co-cultures, xenografts, tissue
Agentes antiagregantes plaquetarios en la prevención secundaria del ictus isquémico

Subjects who have suffered a stroke or a transient ischemic attack (TIA) are at high risk for a recurrent event. Antiplatelet agents are the first line therapy for secondary prevention of ischemic stroke. The present review summarizes the mode of action, efficacy and adverse effects of antiplatelet agents, including the results of different clinical studies in the secondary prevention of ischemic stroke. Antiplatelet agents such as aspirin, ticlopidine, clopidogrel and the combination aspirin-extended-release dipyridamole have been shown to reduce the risk of ischemic stroke after a stroke or TIA. The selection of an antiplatelet agent after suffering a stroke or TIA should be individualized in terms of their efficacy, adverse effects, cost, and patients risk
Extensive re‐modelling of the transpeptidase domain of penicillin‐binding protein 2B of a penicillin‐resistant South African isolate of Streptococcus pneumoniae

Clinical isolates of Streptococcus pneumoniae that have greatly increased levels of resistance to penicillin (>1000‐fold) have been reported from South Africa during the last ten years. Penicillin resistance in these strains is entirely due to the development of penicillin‐binding proteins (PBPs) with decreased affinity for penicillin. We have cloned and sequenced the coding region for the transpeptidase domain of penicillin‐binding protein 2B from three penicillin‐sensitive strains of S. pneumoniae and from a penicillin‐resistant South African strain. The amino acid sequences of the transpeptidase domains of PBP2B of the three penicillin‐sensitive strains were identical and there were only between one and four differences in the nucleotide sequences of their coding regions. The corresponding region of the PBP2B gene from the penicillin‐resistant strain differed by 74 nucleotide substitutions which resulted in 17 alterations in the amino acid sequence of PBP2B. The most remarkable alteration that has occurred during the development of the‘penicillin‐resistant’form of PBP2B is the substitution of seven consecutive residues in a region that is predicted to form a loop at the bottom of the penicillin‐binding site.
Simple test for distinguishing constant from time-varying amplitude in harmonic retrieval problems

In many harmonic retrieval problems, it is not known a priori whether the amplitude of the sinusoidal signals dealt with is constant or time varying. In such applications, a key problem, prior to or in conjunction with frequency estimation, is to decide between these two alternatives or hypotheses. A statistical test for this problem is derived, based on the statistics of the difference between two frequency estimates. The test is computationally efficient and yields, as a by-product, consistent frequency estimates under either hypothesis. Numerical examples are included to show the performance of the proposed test.
Serum gonadotrophin levels in pro-oestrous rats in relation to the resumption of meiosis by the oocytes.

Serum levels of lh, fsh and prolactin were determined in pro-oestrous rats by radioimmunoassay, and the time of commitment of oocytes to undergo maturation was defined by explanting follicles at hourly intervals into a hormone-free medium. All three pituitary hormones showed a rise during the afternoon of pro-oestrus. The prolactin level was still elevated on the morning of oestrus, and serum fsh showed a secondary rise at that time. Administration of pentobarbitone-sodium at 14.00 hours abolished the afternoon rise in the levels of lh and fsh, but not of prolactin, and prevented the induction of oocyte maturation. The percentage of oocytes that completed the first meiotic division when explanted within intact follicles increased during the afternoon of pro-oestrus from <5% at 14.30 to 80% at 17.30 hours. This maturation commitment preceded the dissolution in vivo of the germinal vesicle by about 1\m=1/2\hr. Its attainment closely followed the rising curve of serum
Human dermal microvascular endothelial cells express the 140-kD isoform of neural cell adhesion molecule.

It has only recently been appreciated that the level of gene expression of cell surface markers can be different in endothelial cells derived from different anatomical sites, and that these differences can persist in vitro. In this study, we identify an immunoglobulin gene superfamily member, neural cell adhesion molecule (NCAM), that is expressed on the cell surface of human dermal microvascular endothelial celis but not on umbilical vein, pulmonary vein, aorta, or pulmonary artery derived endothelial cells. By western blot analysis, we identified the 140 kD isoform of NCAM on the surface of human dermal microvascular endothelial cells (HDMEC) derived from dermis. Isolates of HDMEC from human foreskin reproducibly expressed high levels of cell surface immunoreactive protein. In contrast, endothelial cells from large vessels never expressed NCAM constitutively and could not be induced to express NCAM by three proinflammatory cytokines. Western blot analysis of membrane preparations of HDMEC indicated that NCAM protein migrated as a single species with a molecular mass of 140 kD. RT-PCR identified NCAM mRNA in HDMEC cells. The potential for expression of NCAM on small vessels in skin can be interpreted in different ways. Members of the immunoglobulin gene family, including ICAM-1, ICAM-2, and VCAM-1, can be expressed on the cell surface of all endothelial cells and serve as adhesion molecules for leukocytes. It is also possible that, by analogy, NCAM serves as a ligand for a receptor on leukocytes, particularly those that also express NCAM (e.g., natural killer cells). Alternatively, it is possible that NCAM expression permits endothelial cell-cell adhesion, enhancing the structural integrity of microvessels or facilitates neural interactions with microvascular endothelium.
Atención prequirúrgica del hipertenso eventual

Se investigaron pacientes con antecedentes de haber elevado sus cifras tensionales ante situaciones estresantes, pero que habitualmente eran normotensos (hipertensos eventuales). Este tipo de paciente constituye una causa frecuente de suspension del acto quirurgico por hipertension arterial. Se propuso utilizar la prueba de estimulacion por frio, ante la necesidad de identificar el comportamiento tensional operatorio de estos pacientes y por su probada sensibilidad y especificidad como prueba de provocacion de hipertension arterial y equivalente al estres quirurgico, para formular un pronostico de comportamiento tensional transoperatorio. Se trabajo sobre una muestra de 30 sujetos, a todos se les realizo la prueba, 24 tuvieron resultados positivos y se les indico tratamiento individualizado, 6 fueron negativas, no se prescribio tratamiento por no expresar hiperreactividad. No se produjo hipertension perioperatoria, ni suspensiones; se concluyo que la prueba es un valioso instrumento para la prediccion tensional transoperatoria y auxiliar en la desicion de prescribir hipotensores.
Prevalence of Clinical Signs Within Reference Ranges Among Hospitalized Patients Prescribed Antibiotics for Pneumonia

Key Points Question What is the prevalence of antibiotic therapy for possible pneumonia in hospitalized patients despite clinical signs within the reference range? Findings In this cohort study of 12 273 patients treated for possible pneumonia in 4 hospitals, all cardinal signs for pneumonia were within reference ranges in 18.6% of patients with possible community-acquired pneumonia and 13.5% of patients with possible hospital-acquired pneumonia. Antibiotics were continued for 3 days or longer after all clinical signs were normal in 34.8% of patients treated for community-acquired pneumonia and 38.4% treated for hospital-acquired pneumonia. Meaning Findings of this study suggest that antibiotics are prescribed frequently for suspected pneumonia in patients with clinical signs within reference ranges and continued for 3 days or longer after clinical signs normalize; these findings suggest potential targets to improve prescribing.
A Longitudinal Study of the Impact of Group Decision Support Systems on Group Development

Abstract:This paper reports on a study aimed at integrating an important but neglected behavioral issue-group development-into group decision support systems (gdss) research. Group development is based on the fact that most groups have a past and a future, and this affects group outcomes. However, most gdss studies have investigated only a single group session. This study examined the behavior of groups using a gdss over multiple sessions.The study reported in this paper specifically examined the following two questions: (1) does computer support affect the development of decision-making groups? and (2) do the patterns of development differ over time between computer-supported and manual groups? These two questions were studied using a two-factorial repeated measures research design. Results showed significant differences in development patterns between computer-supported groups and manual groups in terms of conflict management and degree of cohesiveness. Mter adapting to gdss, computer-supported groups d.