Miracle-dz/UniFAR-FTUs huggingface dataset:
Epsin 1 is a Polyubiquitin‐Selective Clathrin‐Associated Sorting Protein

In our manuscript published in the March issue of Traffic (1), we cited a recent paper by Chen and De Camilli in the Proceedings of the National Academy of Sciences, USA (2) in support of the proposal that internalization of monoubiquitinated cargo molecules by epsin 1 utilizes a caveolin-dependent internalization pathway. We had interpreted their data, showing that siRNA silencing of clathrin did not prevent the uptake of the plasma membrane-targeted GFP-monoubiquitin reporter protein, to suggest caveolar-dependent uptake. However, it has now been brought to our attention by Pietro De Camilli that nowhere in that paper is this possibility actually suggested. Our own results presented in the Traffic paper, and the conclusions drawn therefrom, are not affected by this unfortunate error. Importantly, the main finding of our study, that the epsin 1 ubiquitin-interacting motifs (UIMs) preferentially bind poly/multiubiquitin and can do so in the context of the assembling clathrin lattice, remains unaltered.
Electrical behaviour of myenteric neurones in the gastric antrum of the guinea‐pig.

1. Intracellular microelectrodes were used to study the electrical behaviour of ganglion cells in the myenteric plexus of the antrum of the guinea‐pig stomach. In the absence of any information on antral myenteric neurones, the aim was to characterize the electrical behaviour and identify biophysical properties of the neurones that could be related to specialized organization of the neural microcircuits in this physiologically important division of the stomach. 2. Myenteric neurones in the gastric antrum were classified into four subtypes based on electrophysiological properties. These were gastric I, II, III and AH/type 2 neurones. Gastric I neurones were characterized by repetitive spike discharge during intraneuronal injection of depolarizing current, by higher input resistances and by lower resting membrane potentials than the other cell types. Gastric II neurones did not discharge repetitively. They discharged one or two spikes only at the beginning of depolarizing current pulses. Gastric III neurones did not discharge action potentials in response to depolarizing pulses. These neurones had higher membrane potentials and lower input resistances than the other types. A fourth type of neurone discharged one or more spikes during depolarizing current pulses and had long‐lasting hyperpolarizing after‐potentials associated with the spikes. The behaviour of these neurones was like AH/type 2 neurones found elsewhere in the enteric nervous system. 3. Action potentials in gastric I and II neurones were abolished by tetrodotoxin. Spikes of the AH/type 2 cells were not abolished by tetrodotoxin due to a calcium component of the inward current. Application of tetraethylammonium broadened the spikes. This was reversed by removal of Ca2+ from the bathing medium. 4. The hyperpolarizing after‐potentials of AH/type 2 neurones were suppressed by removal of Ca2+ from the bathing medium. Treatment with 4‐aminopyridine decreased the amplitude and duration of the after‐hyperpolarization, whereas tetraethylammonium increased the duration and amplitude of the after‐potentials. The hyperpolarizing after‐potentials were unaffected by apamin. 5. Elevation of cyclic 3',5'‐adenosine monophosphate by forskolin resulted in excitation of all AH/type 2 neurones and some of the gastric III cells. Gastric I and II neurones were unaffected. 6. The electrophysiological behaviour of myenteric neurones in the antrum was similar in some respects and different in others from neurones in the gastric corpus and the small and large intestine of the same animal. The differences may reflect distinct organization of the microcircuits for the specialized neural control of the effector functions which characterize the gastric antrum.
Defining the mechanisms of neutrophil exocytosis using proteomic techniques.

DEFINING THE MECHANISMS OF NEUTROPHIL EXOCYTOSIS USING PROTEOMIC TECHNIQUES George Lominadze June 1,2005 Exocytosis of intracellular granules is critical for conversion of inactive, circulating neutrophils to fully activated cells. The p38 MAPK pathway plays a central role in neutrophil exocytosis, although its mechanism of action is unknown. We used several proteomic approaches to identify granule proteins and find targets of the p38 MAPK and its downstream kinase, MK2, on granules. Our analysis identified 286 proteins on neutrophil granules, four of which were known MK2 substrates. Known p38 substrates were not detected. However, MRP-14 was identified as a novel p38 MAPK substrate. MRP-14 was phosphorylated by p38 MAPK in neutrophils upon cell stimulation and translocated to plasma membranes and gelatinase granules, as well as to Triton X-IOO-insoluble structures at the base of lamellipodia. Phosphorylation of the MRP-14 by p38 MAPK increased binding to actin in vitro. These results suggest that MRP-14 is a potential mediator of p38 MAPK-dependent exocytosis in human neutrophils.
Towards Hybrid Model for Human-Inguna

Human-computer interaction, especially in form of dialogue systems and chatbots, has become extremely popular during the last decade. The dominant approach in the recent development of practical virtual assistants is the application of deep learning techniques. However, in case of less resourced language (or domain), the application of deep learning could be very complicated due to the lack of necessary training data. In this paper, we discuss possibility to apply hybrid approach to dialogue modelling by combining data-driven approach with the knowledge-based approach. Our hypothesis is that by combining different agents (general domain chatbot, frequently asked questions module and goal oriented virtual assistant) into single virtual assistant we can facilitate adequacy and fluency of the conversation. We investigate suitability of different widely used techniques in less resourced settings. We demonstrate feasibility of our approach for morphologically rich less resourced language Latvian through initial virtual assistant prototype for the student service of the University of Latvia.
A Lignan from Alnus japonica Activates Myogenesis and Alleviates Dexamethasone-induced Myotube Atrophy

Abstract To find inhibitors against skeletal muscle loss, we isolated a lignan compound ((−)-(2 R ,3 R -1,4-O-diferuloylsecoisolarciresinol, DFS) from the stem of Alnus japonica . C2C12 myoblasts were treated with DFS during differentiation. To induce an in vitro atrophic condition, differentiated myotubes were treated with dexamethasone (a synthetic glucocorticoid). DFS (10 nM) increased expression levels of myogenic factors and the number of multi-nucleated myotubes expressing myosin heavy chain (MHC). The myogenic potential of DFS could be attributed to p38 MAPK activation. DFS also protected against dexamethasone-induced damage, showing increased expression of MHC and mammalian target of rapamycin (mTOR), a major anabolic factor. Under atrophic condition, the anti-myopathy effect of DFS was associated with inactivation of NF- κ B signaling pathway and the subsequent suppression of muscle degradative E3 ligases and myostatin. DFS treatment also restored fast muscle fiber (type II a, II b, and II x), known to be susceptible to dexamethasone. These results indicate that DFS isolated from A. japonica can stimulate myogenesis via p38 MAPK activation and alleviate muscle atrophy by modulating the expression of genes associated with muscle protein anabolism/catabolism. Thus, we propose that DFS can be used as a pharmacological and nutraceutical agent for increasing muscle strength or protecting muscle loss.
A Review of Android Security System

-Android operating system uses the permission-based model which allows Android applications to access user information, system information, device information and external resources of Smartphone. The developer needs to declare the permissions for the Android application. The user needs to accept these permissions for successful installation of an Android application. These permissions are declarations. At the time of installation, if the permissions are allowed by the user, the app can access resources and information anytime. It need not request for permissions again. Android OS is susceptible to various security attacks due to its weakness in security. This paper tells about the misuse of app permissions using Shared User ID, how two factor authentications fail due to inappropriate and improper usage of app permissions using spyware, data theft in Android applications, security breaches or attacks in Android and analysis of Android, IOS and Windows operating system regarding its security.
Tourists’ understanding of sustainable tourism: An analysis in eight countries Version1.1 PRELIMINARY VERSION Authors

The goal of this study is to learn more about tourists’ understanding of sustainable tourism. The empirical survey with 6,000 respondents in eight countries identifies the most relevant aspects of sustainable tourism from a tourists’ perspective. Overall the perception is balanced over the different dimensions. Furthermore, five different types regarding tourists’ understanding of sustainable tourism are identified in a cluster analysis and a potential market size of sustainable tourism of 22% of all tourists can be identified.
Uranium-lead geochronology of eastern Meta Incognita Peninsula, southern Baffin Island, Nunavut

This paper presents new U-Pb geochronology data from three sedimentary rocks and three plutonic rocks collected during recent bedrock geological mapping of eastern Meta Incognita Peninsula, Baffin Island, Nunavut. A quartzite (sample 14SAB-S013A), contiguous with the type area of the Lake Harbour Group near Kimmirut, is characterized by an exclusively Archean detrital zircon profile, dominated by ca. 2.82 Ga detritus. Calcareous quartzite from the central part of the peninsula (sample 14SAB-O103B) includes detrital ages of 2.7 Ga, 2.6–2.5 Ga, 2.35 Ga, 2.1 Ga and 1.93–1.91 Ga. The maximum age of deposition of the calcareous quartzite is 1913 ±17 Ma. A grey, sandy quartzite horizon (sample 14SABR108B), from an outcrop of psammite/leucogranite, is dominated by Orosirian age (2050–1800 Ma) zircon. A component of ca. 1.85 Ga zircon is interpreted to reflect partial melting and emplacement of nearby leucogranite. Given their proximity to the type area, and similar lithological association and geological setting, the metasedimentary rocks on eastern Meta Incognita Peninsula are correlated with the Lake Harbour Group. A megacrystic biotite-orthopyroxene monzogranite (sample 14SAB-O071A) is interpreted to have crystallized at 1845 ±19 Ma. A megacrystic biotite-magnetite monzogranite (sample 14SAB-R62A) is interpreted to have a crystallization age of 1865 ±10 Ma, with an older 1896 Ma component representing an inherited or possibly autocrystic phase. A pink, sugarytextured biotite-magnetite monzogranite (sample 14SAB-O049A) is cautiously interpreted to have crystallized at 1871 ±4 Ma followed by metamorphism that resulted in resetting of zircon U-Pb systematics. Despite their relatively simple and homogeneous appearance in the field, complex geochronological results made interpretation of the crystallization ages of plutonic phases from Meta Incognita Peninsula challenging. The highest grade, most deformed samples discussed in this paper yielded the youngest ages. This emphasizes the need for careful field observations of crosscutting relationships when establishing a field chronology for plutonic rocks.
Determination of individual teleroentgenographic characteristics of the lower medial incisors position in Ukrainian young men and young women with orthognathic bite

Ethnic, sexual and age features of the incisors positions point to the need for the development of techniques that allow to determine their individual characteristics, since commonly accepted standards recommended for optimal positioning of incisors can only be used as indicative. The purpose of the study by studying teleroentgenographic indices and conducting direct stepwise regression analysis in young men and young women of Ukraine with orthognathic bite, to develop and analyze the mathematical models of individual characteristics of the position of the lower medial incisors. With the Veraviewepocs 3D device, Morita (Japan) in 38 young men (17 to 21 years of age) and 55 young women (aged from 16 to 20 years) with occlusion close to orthognathic bite and balanced faces received side teleroentgenograms. Cephalometric analysis was performed using OnyxCephTM software. Cephalometric points and measurements were performed according to the recommendations of A.M. Schwarz, J. McNamara, W.B. Downs, R.A. Holdway, P.F. Schmuth, C.C. Steiner and C.H. Tweed. According to the above methods, in the licensed package "Statistica 6.0" using direct straight line regression analysis, the teleroentgenographic characteristics of the position of the lower medial incisors (distance 1l_NB, distance 1l_APog, angle 1l_DOP, IMPA angle, Mand1_NB angle, FMIA angle and Mand1_MeIm angle) were performed. In young men with orthognathic bite of 7 possible models of teleroentgenographic characteristics of the lower medial incisors, 5 were constructed with determination coefficient R from 0.694 to 0.849, and in young women, all 7 possible models with determination coefficient R from 0.595 to 0.794. In young men most often the regression equations included the angle ANB and facial vertical index GL_SN_S (by 11.5%); lower face height ANS_ME, face angle NBA_PTGN and distance S_E (by 7.7%). In young women most often the regression equations included the angle of N_POG (16.7%); Wits indicator (13.9%); inclination angle I (8.3%); H-angle, maxillo-mandibular angle MM and angle of facial axis NBA_PTGN (by 5.6%). Thus, in the work with the help of the method of stepwise regression with inclusion, among Ukrainians of juvenileage, on the basis of features teleroentgenographic indicators, the analysis of reliable models of individual teleroentgenographic characteristics of the position of the lower medial incisors was developed and carried out.
Knockdown of KCNQ1OT1 attenuates cardiac hypertrophy through modulation of the miR-2054/AKT3 axis

Background Persistent cardiac hypertrophy threatens health worldwide. Long non-coding RNAs (lncRNAs) attracted lots of attention in cardiac diseases such as cardiac hypertrophy. In this study, we aimed to study the function of KCNQ1OT1 in cardiac hypertrophy. Methods We first used qRT-PCR to detect the expression of KCNQ1OT1 in Ang-II-induced cardiomyocytes and mouse cardiac hypertrophy models. The function of KCNQ1OT1 was investigated by a loss-of-function test. Analysis of the luciferase reporter gene and RNA pulldown confirmed the interaction between KCNQ1OT1 and miR-2054. The target gene of miR-2054 was predicted by bioinformatics analysis and confirmed by luciferase reporter gene detection. Rescue experiments were performed to evaluate the role of miR-2054/AKT3 in the function of KCNQ1OT1. Results Our results suggested that KCNQ1OT1 was up-regulated in Ang-II-induced cardiomyocytes and transverse aortic constriction (TAC) mice. Knocking down of KCNQ1OT1 can reduce cell size and downregulate the expression of ANF, BNP and α-MHC in response to Ang-II. KCNQ1OT1 has been shown to compete competitively with miR-2054 and has a negative correlation with its expression. The combination of miR-2054 can reverse the effect of the KCNQ1OT1 combination in Ang-II-induced cardiomyocytes. In addition, AKT3 is a target of miR-2054 and mediates its effect on Ang-II-induced cardiomyocytes. Conclusions Knockdown of KCNQ1OT1 could attenuate cardiac hypertrophy through modulation of the miR-2054/AKT3 axis.
INFLUENCE OF BENTONITE , DOLOMITE , NATURAL ZEOLITE AND MANURE ON HEAVY METAL IMMOBILIZATION IN A CONTAMINATED SOIL

Using organic or inorganic amendments to immobilize the heavy metals in soils is a cost-effective remediation technique for contaminated soils. The aim of study was to assess the efficiency of 4 amendments to immobilize cadmium (Cd), copper (Cu), lead (Pb) and zinc (Zn) in soil and reduce their phyto availabilty. An experimental field was set-up in Copșa Mica area and four amendments were applied: Na-bentonite, dolomite, natural zeolite and manure. Following the application of the amendments, a mix of perennial grasses and straw cereals, belonging to the family of Gramineae, were sown. The efficiency of in situ immobilization of heavy metals was assessed using DTPA extraction of metals and plant uptake. All treatments produced significant increases of pH values in soil. The mean soil pH of the control plots was 5.18. Comparing the effects of treatments on soil reaction, the best results were observed after applications of bentonite or dolomite. The highest mean value of pH was measured in plots treated with Na-bentonite (pH 7.16). The bioavailability of metals is related to soil pH. Therefore the effects of treatments on pH are reflected on metals bioavailability assessed by DTPA-extractable metal fractions. All amendments caused a reduction of heavy metal (Cu, Cd, Pb or Zn) mobility compared to the control but the magnitude of effects depends on metal and amendment. For copper and zinc, addition of Na-bentonite reduces statistically significant the mobile forms in soil from 13.6 mg·kg Cu (control) to 9.4 mg·kg Cu and from 327 mg·kg Zn (control) to 143 mg·kg Zn. By using the natural zeolite the values of heavy metal contents in mobile forms in soil decreased significantly comparing with control. The results demonstrate the high potential of studied amendments for immobilization of heavy metals in contaminated agricultural soils.
Evaluation of Satisfaction and Trust based on Service Quality for Flight Passenger, Indonesia

The purpose of this study is to examine the relationship between service quality to satisfaction and trust, also to examine influence of reciprocal between satisfaction and trust. The uniqueness of this study is to test the quality of service to trust for the service sector. No previous research has been found that examines the direct effect of service quality and trust, particularly in the goods care services sector. Research location at Adisutjipto Airport Yogyakarta. Research respondents are passengers of Garuda Indonesia Airlines who utilize luggage storage services handled by a lost and fund unit is 99 respondents as research sample. Questionnaire as a tool to collect primary data. The research design is quantitative with regression techniques to test the modeling. The results indicate that service quality is stronger leads to trust than satisfaction for service users. There is a reciprocal influence between satisfaction and trust. The key finding of this study is the guarantee of passenger luggage for no damage and loss of positive impact on passenger trust.
Study on protein and amino acid requirements in different crossbreeds of swine

Hybrids and different breeds of swine have distinct capacity for productivity and therefore have different nutrient requirements to satisfy their needs. Contemporary swine breeds had high genetic potential for gain and deposition of more meat which made them sensitive to the lack of protein and amino acids. Determination of protein and amino acid requirements in recently created crossbreeds was an important factor contributing to the determination of their capacity for optimal gain and utilization of feed. When establishing the requirements for new origins, the defining factors in the feeding strategy included genotype, type of used feed, protein levels in rations providing a reduction of nitrogen pollution in the environment, protein nutrition, content of intramuscular fats in carcasses and meat quality.
Comparison Between the Contents of Phenolic and Flavonoid Compounds and Aerial Part Antioxidant Activity in Scutellaria pinnatifida in Two NorthIranian Populations

Background and Objective: Phenolic acids, polyphenols and flavonoids are antioxidant compounds, which act as cell protective factors against oxidative stress by trapping and inhibition of free radicals, play an important role in plants and animals health. In this research, in order to optimize extraction of antioxidant compounds, phenolic and flavonoid contents and antioxidant activity of Scutellaria pinnatifida aerial part were determined. Materials and Methods: Dried powder of aerial part from two populations, Dizin and Kojur, was extracted with ethanol 80%, methanol 80%, water and ethyl acetate, by two soaking and sonication methods. Then, the contents of total phenolic and flavonoid compounds were assayed by spectrophotometric methods. Antioxidant activity of the extracts were analyzed by two tests: free radical scavenging activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and bleaching β -carotene in linoleic acid system. Statistical analysis of the data was performed by One-way and two-way ANOVA to compare the means. Results: The highest content of total phenol and flavonoid (8 and 24 mg/g Shoot dry weight, respectively) was obtained by soaking method and ethanolic extraction in Kojur population. While in Dizin population with the same extraction techniques, the contents of the mentioned compounds were at very low levels (5.6 and 1.6 mg/g DW, respectively). IC 50 value of the ethanolic, methanolic and aqueous extracts were estimated about 0.1- 1.2 mg/ml in the Kojur population. The phenolic and flavonoid contents of these extracts were significantly higher than ethyl acetate extracts. With the exception of aqueous extract prepared by soaking, scavenging capacity of DPPH free radicals by different extracts of Dizin population was considerably lower than the Kojour population. The antioxidant assay results obtained from beta-carotene-linoleic acid test showed that the highest inhibition percentage of lipid peroxidation resulted from aqueous extracts prepared by ultrasonic waves (respectively 93% and 50% inhibition by extracts of the Kojour and Dizin populations). Conclusion: The means of total phenolic and flavonoid contents of the extracts were significantly different under the influence of three factors: population, extraction method and solvent. The highest antioxidant activity was observed in Kojur population: by soaking methanolic extraction method in DPPH test or by sonicate aqueous extraction method in the inhibition of lipid peroxidation test.