Miracle-dz/UniFAR-FTUs huggingface dataset:
Early prenatal diagnosis of alveolar capillary dysplasia with misalignment of pulmonary veins due to a 16q24.1 deletion

First trimester ultrasound screening is an essential fetal examination performed generally at 11–13 weeks of gestation (WG). However, it does not allow for an accurate description of all fetal organs, partly due to their development in progress. Meanwhile, increased nuchal translucency (INT) is a widely used marker known to be associated with chromosomal deleterious rearrangements. We report on a 14 WG fetus with an association of INT and univentricular congenital heart malformation (CHM) leading to chorionic villous sampling (CVS). Cytogenetic investigations performed using array‐Comparative Genomic Hybridization (CGH) and fluorescence in situ hybridization (FISH) demonstrated a 1.17 Mb deletion in 16q24.1 encompassing FOXF1 arisen de novo on maternal inherited chromosome. Fetopathological study confirmed CHM with hypoplastic left heart syndrome (HLHS) associating aortic atresia, mitral stenosis, and left ventricular hypoplasia and revealed in addition specific lung lesions corresponding to alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV). This is so far the first case of first trimester prenatal diagnosis of ACDMPV due to the deletion of FOXF1 gene. An interpretation of the complex genomic data generated by ultrasound markers is facilitated considerably by the genotype–phenotype correlations on fetopathological examination.
Effect of Active-Edible Coating and Essential Oils on Lamb Patties Oxidation during Display

The use of natural products to reduce the use of synthetic additives in meat products, reducing the oxidation and improving the shelf life is a current challenge. Meat quality from lamb patties during 10 days of display on modified atmosphere packaging (MAP) and active-edible coating were tested under six treatments: uncoated patties without coating (CON); patties with alginate coating (EC) and patties with coating and 0.1 or 0.05% of essential oils (EOs) from either thyme (TH 0.1; TH 0.05) or oregano (OR 0.1; OR 0.05). Display and treatment significantly modified (P < 0.001) all the studied meat quality variables (pH, color, water holding capacity, weight losses, thiobarbituric acid reactive substances (TBARS), antioxidant activity). Display produced discoloration and lipid oxidation, however, the samples with essential oils presented lower (P < 0.001) lipid oxidation than the CON or EC groups. Coated samples with or without EOs showed better color (lower lightness but higher redness and yellowness) and lower water losses (P < 0.001) than the CON. The addition of thyme EO caused a decrease (P < 0.001) in the consumer’s overall acceptability, whereas no statistical differences appeared between CON, EC and oregano EO addition. Thus, using EOs as natural antioxidants, especially those from oregano at low dosages (0.05%), could be considered a viable strategy to enhance the shelf life and the product quality of lamb meat patties without damaging the sensory acceptability.
Adverse childhood experiences and transcriptional response in school-age children

Abstract This study evaluated whether children with higher adverse childhood experiences (ACE) scores had alterations in immune cell gene expression profiles. RNA sequencing was conducted on dried blood spot samples from 37 generally healthy English-speaking children (age 5–11) who were recruited from well-child visits at a university-affiliated pediatric practice. The Whole Child Assessment was used to assess ACE exposure. Primary analyses examined an a priori-specified composite of 19 pro-inflammatory gene transcripts. Secondary analyses examined a 34-gene composite assessing Type I interferon response, and used Transcript Origin Analyses to identify cellular mechanisms. After controlling for age, body mass index percentile, sex, race/ethnicity, current insurance status, and household smoking exposure, pro-inflammatory gene expression was elevated by 0.094 log2 RNA expression units with each Child-ACE total score point (p = .019). Type I interferon gene expression was similarly upregulated (0.103; p = .008). Transcript origin analyses implicated CD8+ T cell as the primary sources of gene transcripts upregulated, and nonclassical (CD16+) monocytes as sources of downregulated transcripts. These preliminary analyses suggest that parent-reported ACE exposures are associated with increased expression of both inflammatory and interferon gene transcripts in children's circulating blood cells.
Strategic approach to minimally invasive necrosectomy for necrotizing pancreatitis: technique, complications and predictors of outcome

Minimally invasive retroperitoneal necrosectomy has been an integral component of ‘step‐up’ approach for infected pancreatic necrosis. Even though the clinical outcome of nephroscopic necrosectomy has been studied earlier, its predictor and morbidities following surgery have not been extensively evaluated. We aimed to evaluate the clinical outcome and early and late complications after percutaneous nephroscopic necrosectomy (PCNN).
Efficacy of daily GnRH agonist for luteal phase support following GnRH agonist triggered ICSI cycles versus conventional strategy: A Randomized controlled trial

Objective: The use of gonadotropin-releasing hormone agonist (GnRHa) as an alternative for human chronic gonadotropin (hCG) trigger has potential benefits, but the optimal luteal phase support (LPS) following GnRHa trigger remains to be elucidated. We aimed to investigate a new strategy (daily GnRH agonist for LPS following GnRH agonist trigger) as an alternative for the conventional approach to the patients undergoing intracytoplasmic sperm injection (ICSI). Methods: In this randomized controlled trial study, 44 ICSI patients were randomly assigned into two groups: group 1, patients received standard strategy (hCG trigger [10000 IU] and progesterone bid [400 mg/BD] for LPS); group 2, patients received a dose of GnRHa (0.2 mg) for ovulation trigger and subcutaneous injection of GnRHa bid (0.2 mg) for LPS. Results: The pregnancy, miscarriage, and live birth rates for the patients undergoing LPS following the GnRHa trigger were similar to those of patients undergoing the standard strategy. Conclusions: We showed that a daily subcutaneous injection of GnRHa for LPS following the GnRHa trigger can be successfully performed as an alternative to the standard strategy, with comparable pregnancy and live birth rates in ICSI patients.
Understanding the Effects of Anesthesia on Cortical Electrophysiological Recordings: A Scoping Review

General anesthesia in animal experiments is an ethical must and is required for all the procedures that are likely to cause more than slight or momentary pain. As anesthetics are known to deeply affect experimental findings, including electrophysiological recordings of brain activity, understanding their mechanism of action is of paramount importance. It is widely recognized that the depth and type of anesthesia introduce significant bias in electrophysiological measurements by affecting the shape of both spontaneous and evoked signals, e.g., modifying their latency and relative amplitude. Therefore, for a given experimental protocol, it is relevant to identify the appropriate anesthetic, to minimize the impact on neuronal circuits and related signals under investigation. This review focuses on the effect of different anesthetics on cortical electrical recordings, examining their molecular mechanisms of action, their influence on neuronal microcircuits and, consequently, their impact on cortical measurements.
The 63 K phase transition of ZrTe3: a neutron diffraction study

The 63 K phase transition of ZrTe 3 has been followed through SQUID magnetisation and neutron diffraction studies. The transition is characterized by a small quenching of the magnetic susceptibility. Contrary to the expectation that the structural transition is associated with a charge density wave, the results of Rietveld refinements of high resolution neutron powder diffraction profiles indicate that below the phase transition the different bonding Te-Te contacts are more equal rather than less. The results are examined with the help of band structure calculations on structures determined at three different temperatures. The picture that emerges supports the view that anion-cation redox competition plays a crucial role in determining not only the structures of these compounds, but also the temperature dependence thereof.
Efficacy of cryoablation in idiopathic and non-idiopathic atrial fibrillation patients.

OBJECTIVES AND BACKGROUND Studies assessing the unicentric and prospective comparison of efficacy of cryoballoon pulmonary vein isolation in idiopathic and non-idiopathic atrial fibrillation (AF) patients are missing. The aim of this study was to compare a single procedural outcome in these subgroups of AF patients. METHODS A total of 208 patients with drug resistant AF were included in this study. Among them, 36 patients had idiopathic AF and 172 patients had non-idiopathic AF. The efficacy endpoint was freedom from any atrial arrhythmia lasting >30 s after a 3-months blanking period. RESULTS The freedom from atrial arrhythmias without antiarrhythmic drugs after 1 and 3 years of follow-up, respectively, was 85.2 % and 70.4 % in patients with idiopathic AF; and 64.6 % and 39.9 % in patients with non-idiopathic AF (p=0.021). Moreover, the success rate when analysing only paroxysmal AF patients after 1 and 3 years of follow-up, respectively, was 95.7 % and 82 % in patients with idiopathic AF; and 72.6 % and 47 % in patients with non-idiopathic AF (p=0.022). CONCLUSIONS A single cryoballoon pulmonary vein isolation was more effective in preventing atrial arrhythmias relapses in idiopathic AF patients compared to non-idiopathic AF patients. The best efficacy outcomes were observed in patients with paroxysmal idiopathic AF (Tab. 5, Fig. 2, Ref. 25).
Towards Detecting Biceps Muscle Fatigue in Gym Activity Using Wearables

Fatigue is a naturally occurring phenomenon during human activities, but it poses a bigger risk for injuries during physically demanding activities, such as gym activities and athletics. Several studies show that bicep muscle fatigue can lead to various injuries that may require up to 22 weeks of treatment. In this work, we adopt a wearable approach to detect biceps muscle fatigue during a bicep concentration curl exercise as an example of a gym activity. Our dataset consists of 3000 bicep curls from twenty middle-aged volunteers at ages between 27 to 30 and Body Mass Index (BMI) ranging between 18 to 28. All volunteers have been gym-goers for at least 1 year with no records of chronic diseases, muscle, or bone surgeries. We encountered two main challenges while collecting our dataset. The first challenge was the dumbbell’s suitability, where we found that a dumbbell weight (4.5 kg) provides the best tradeoff between longer recording sessions and the occurrence of fatigue on exercises. The second challenge is the subjectivity of RPE, where we average the reported RPE with the measured heart rate converted to RPE. We observed from our data that fatigue reduces the biceps’ angular velocity; therefore, it increases the completion time for later sets. We extracted a total of 33 features from our dataset, which have been reduced to 16 features. These features are the most overall representative and correlated with bicep curl movement, yet they are fatigue-specific features. We utilized these features in five machine learning models, which are Generalized Linear Models (GLM), Logistic Regression (LR), Random Forests (RF), Decision Trees (DT), and Feedforward Neural Networks (FNN). We found that using a two-layer FNN achieves an accuracy of 98% and 88% for subject-specific and cross-subject models, respectively. The results presented in this work are useful and represent a solid start for moving into a real-world application for detecting the fatigue level in bicep muscles using wearable sensors as we advise athletes to take fatigue into consideration to avoid fatigue-induced injuries.
A modifier screen identifies regulators of cytoskeletal architecture as mediators of Shroom-dependent changes in tissue morphology

ABSTRACT Regulation of cell architecture is critical in the formation of tissues during animal development. The mechanisms that control cell shape must be both dynamic and stable in order to establish and maintain the correct cellular organization. Previous work has identified Shroom family proteins as essential regulators of cell morphology during vertebrate development. Shroom proteins regulate cell architecture by directing the subcellular distribution and activation of Rho-kinase, which results in the localized activation of non-muscle myosin II. Because the Shroom-Rock-myosin II module is conserved in most animal model systems, we have utilized Drosophila melanogaster to further investigate the pathways and components that are required for Shroom to define cell shape and tissue architecture. Using a phenotype-based heterozygous F1 genetic screen for modifiers of Shroom activity, we identified several cytoskeletal and signaling protein that may cooperate with Shroom. We show that two of these proteins, Enabled and Short stop, are required for ShroomA-induced changes in tissue morphology and are apically enriched in response to Shroom expression. While the recruitment of Ena is necessary, it is not sufficient to redefine cell morphology. Additionally, this requirement for Ena appears to be context dependent, as a variant of Shroom that is apically localized, binds to Rock, but lacks the Ena binding site, is still capable of inducing changes in tissue architecture. These data point to important cellular pathways that may regulate contractility or facilitate Shroom-mediated changes in cell and tissue morphology. Summary: Using Drosophila as a model system, we identify F-actin and microtubules as important determinants of how cells and tissues respond to Shroom induced contractility.
Clinical Outcomes and Patient-Matched Molecular Composition of Relapsed Medulloblastoma

PURPOSE We sought to investigate clinical outcomes of relapsed medulloblastoma and to compare molecular features between patient-matched diagnostic and relapsed tumors. METHODS Children and infants enrolled on either SJMB03 (NCT00085202) or SJYC07 (NCT00602667) trials who experienced medulloblastoma relapse were analyzed for clinical outcomes, including anatomic and temporal patterns of relapse and postrelapse survival. A largely independent, paired molecular cohort was analyzed by DNA methylation array and next-generation sequencing. RESULTS A total of 72 of 329 (22%) SJMB03 and 52 of 79 (66%) SJYC07 patients experienced relapse with significant representation of Group 3 and wingless tumors. Although most patients exhibited some distal disease (79%), 38% of patients with sonic hedgehog tumors experienced isolated local relapse. Time to relapse and postrelapse survival varied by molecular subgroup with longer latencies for patients with Group 4 tumors. Postrelapse radiation therapy among previously nonirradiated SJYC07 patients was associated with long-term survival. Reirradiation was only temporizing for SJMB03 patients. Among 127 patients with patient-matched tumor pairs, 9 (7%) experienced subsequent nonmedulloblastoma CNS malignancies. Subgroup (96%) and subtype (80%) stabilities were largely maintained among the remainder. Rare subgroup divergence was observed from Group 4 to Group 3 tumors, which is coincident with genetic alterations involving MYC, MYCN, and FBXW7. Subgroup-specific patterns of alteration were identified for driver genes and chromosome arms. CONCLUSION Clinical behavior of relapsed medulloblastoma must be contextualized in terms of up-front therapies and molecular classifications. Group 4 tumors exhibit slower biological progression. Utility of radiation at relapse is dependent on patient age and prior treatments. Degree and patterns of molecular conservation at relapse vary by subgroup. Relapse tissue enables verification of molecular targets and identification of occult secondary malignancies.
Single Cell Analysis of Bistable Expression of Pathogenicity Island 1 and the Flagellar Regulon in Salmonella enterica

Bistable expression of the Salmonella enterica pathogenicity island 1 (SPI-1) and the flagellar network (Flag) has been described previously. In this study, simultaneous monitoring of OFF and ON states in SPI-1 and in the flagellar regulon reveals independent switching, with concomitant formation of four subpopulations: SPI-1OFF FlagOFF, SPI-1OFF FlagON, SPI-1ON FlagOFF, and SPI-1ON FlagON. Invasion assays upon cell sorting show that none of the four subpopulations is highly invasive, thus raising the possibility that FlagOFF cells might contribute to optimal invasion as previously proposed for SPI-1OFF cells. Time lapse microscopy observation indicates that expression of the flagellar regulon contributes to the growth impairment previously described in SPI-1ON cells. As a consequence, growth resumption in SPI-1ON FlagON cells requires switching to both SPI-1OFF and FlagOFF states.
Phenolic Compounds Impact on Rheumatoid Arthritis, Inflammatory Bowel Disease and Microbiota Modulation

Non-communicable chronic diseases (NCDs) are nowadays the principal cause of death, especially in most industrialized nations. These illnesses have increased exponentially with the consumption of diets very high in fat and sugar, not to mention stress and physical inactivity among other factors. The potential impact of suboptimal diets on NCDs’ morbidity and mortality rates brings to the forefront the necessity for a new way of improving dietary habits. The literature provides extensive scientific work that presents evidence that phenolic compounds from diets have antioxidant, anti-inflammatory and antiproliferative activities that impact human health. Gut microbiota modulation by some phenolic compounds leads to favorable changes in abundance, diversity, and in the immune system. However, polyphenol’s limited bioavailability needs to be overcome, highlighting their application in new delivery systems and providing their health benefits in well-established ways such as health maintenance, treatment or adjuvant to conventional pharmacological treatments. In this context, novel dietary approaches, including new food supplements, have emerged to prevent diseases and preserve health.
Can We Use Grip Strength to Predict Other Types of Hand Exertions? An Example of Manufacturing Industry Workers

Background: There are different types of hand motions in people’s daily lives and working environments. However, testing duration increases as the types of hand motions increase to build a normative database. Long testing duration decreases the motivation of study participants. The purpose of this study is to propose models to predict pinch and press strength using grip strength. Methods: One hundred ninety-eight healthy volunteers were recruited from the manufacturing industries in Central Taiwan. The five types of hand motions were grip, lateral pinch, palmar pinch, thumb press, and ball of thumb press. Stepwise multiple linear regression was used to explore the relationship between force type, gender, height, weight, age, and muscle strength. Results: The prediction models developed according to the variable of the strength of the opposite hand are good for explaining variance (76.9–93.1%). Gender is the key demographic variable in the predicting models. Grip strength is not a good predictor of palmar pinch (adjusted-R2: 0.572–0.609), nor of thumb press and ball of thumb (adjusted-R2: 0.279–0.443). Conclusions: We recommend measuring the palmar pinch and ball of thumb strength and using them to predict the other two hand motions for convenience and time saving.
Implementasi Algoritma Load Balancing PLBA Komputasi Grid pada Lab Environment Menggunakan PVM3

Load balancing is one of the main parts of scheduling Grid resources. One of the load balancing models on Grid resources is the hierarchical model. This model has the advantage that it requires minimal communication costs between one resource and another. The PLBA load balancing algorithm uses a hierarchical model with dynamically obtained threshold values, so that it can adjust conditions at a time, both the state of the resource, the state of the computer network, and the state of the recipient or client. PVM3 is a software system capable of optimizing heterogeneous resources, so that resources can work in parallel. Resources can also complete tasks well, even though they are very large and complex tasks. This research has implemented the PLBA load balancing algorithm, with the aim of optimizing Grid resources. This research has also developed the PLBA load balancing algorithm by changing the arguments for NPEList, so that resources can be grouped more optimally. The PLBA load balancing algorithm has been successfully developed by modifying the arguments for NPEList, so that the running time required to complete the given tasks is shorter, because resources can be grouped more optimally. This has been shown by the shorter average running time when using the modified NPEList argument (0.75 * threshold1 <= ALCi <= 1.25 * threshold1) is shorter, than using the NPEList argument in previous research (ALCi = threshold1). Comparison of the average running time has been obtained as follows : (82513.63740 : 67837.71720); (63869.92450 : 50722.17210); (858,96710 : 207,33680); (321.88000 : 126.89100); (768.54560 : 468.27190); (780.22770 : 279.43730).
MOLECULAR DETECTION OF EIMERIA SPP. IN DEER AT MIDDLE PARTS OF IRAQ

Eimeria is an apicomplexan protozoa which affect many species of wild and domestic animals and birds including deer. The study was designed for investigation Eimeria spp. in deer by Molecular methods for the first time in Iraq and Globally. One hundred fecal samples of deer were examined by Conventional PCR from different provinces in the middle part of Iraq during December 2018, to end of September 2019. A total rate of infection were 77% (77/100). Male and female recorded 63.6 % (21/33) and 83.5 % (56/67) rate of infection respectively significant differences existed (p≥ 0.05). There were significant differences in the rate of infection between age groups of deer were recorded in Fawn deer (<3-11 months) which 82.6% (19/14), while the lowest recorded in adult deer at age group (11-20 months) which 72.2% (26/36). The rate of infection by Conventional PCR were recorded at four provinces. Highest rate was recorded in Baghdad 80.5% (30/36) and lowest rate was in Babylon 72% (8/11). The rate of infection with Eimeria spp. deer relation to months of study. The results showed that the highest infection rate recorded during March 90.9% (10/11) and the lowest rate recorded during December 64 %(7/11) with the significant differences. Nine species of Eimeria (E. abenovi, E. crandalis, E. elegans, E. gazella, E. idmii, E. intricate, E. rheemi, E. parva and E. sordida) by sequencing of 40 deer fecal samples isolate for the first time in Iraq and global.