Miracle-dz/UniFAR-FTUs huggingface dataset:
Dual regulation of translation initiation and peptide chain elongation during BDNF‐induced LTP in vivo: evidence for compartment‐specific translation control

Protein synthesis underlying activity‐dependent synaptic plasticity is controlled at the level of mRNA translation. We examined the dynamics and spatial regulation of two key translation factors, eukaryotic initiation factor 4E (eIF4E) and elongation factor‐2 (eEF2), during long‐term potentiation (LTP) induced by local infusion of brain‐derived neurotrophic factor (BDNF) into the dentate gyrus of anesthetized rats. BDNF‐induced LTP led to rapid, transient phosphorylation of eIF4E and eEF2, and enhanced expression of eIF4E protein in dentate gyrus homogenates. Infusion of the extracellular signal‐regulated kinase (ERK) inhibitor U0126 blocked BDNF‐LTP and modulation of the translation factor activity and expression. Quantitative immunohistochemical analysis revealed enhanced staining of phospho‐eIF4E and total eIF4E in dentate granule cells. The in vitro synaptodendrosome preparation was used to isolate the synaptic effects of BDNF in the dentate gyrus. BDNF treatment of synaptodendrosomes elicited rapid, transient phosphorylation of eIF4E paralleled by enhanced expression of α‐calcium/calmodulin‐dependent protein kinase II. In contrast, BDNF had no effect on eEF2 phosphorylation state in synaptodendrosomes. The results demonstrate rapid ERK‐dependent regulation of the initiation and elongation steps of protein synthesis during BDNF‐LTP in vivo. Furthermore, the results suggest a compartment‐specific regulation in which initiation is selectively enhanced by BDNF at synapses, while both initiation and elongation are modulated at non‐synaptic sites.
Enhanced predictive current control method for the asymmetrical dual—three phase induction machine

The interest in multiphase drives has re—emerged in the last decade, being the asymmetrical dual three—phase induction motor drive one of the most popular options. Predictive control techniques have been recently implemented in these multiphase drives. Proposed schemes have demonstrated high performance at the expenses of high computational cost, unknown switching frequency and the appearance of large undesirable stator current harmonic components. In this paper, an enhanced predictive current control technique (ePCC) with fixed switching frequency is presented for the asymmetrical dual three—phase ac drive. Fast torque and current response is achieved, and stator current harmonic suppression is favored. Experimental results are provided to examine the benefits of the proposed control method.
A Justesen construction of binary concatenated codes that asymptotically meet the Zyablov bound for low rate

An explicit construction of a sequence of binary codes that asymptotically meet the Zyablov bound for rates lower than 0.30 is given by using Justesen's construction of concatenation. The outer codes are constructed from generalized Hermitian curves. These outer codes can be described without any algebraic geometry terminology, while the proofs of some properties deeply rely on algebraic geometry. >
Adolescence: psychopathological discomfort, distress or psychiatric disorder? Clinical experience, description and analysis of a sample.

BACKGROUND The aim of this study was to highlight how many psychopathological disorders emerging during adolescence are not easily detectable and interpretable. METHODS We investigated the social, emotional and demographic factors related to psychopathological distress, measuring the overall functioning of the child/adolescent and the consequent impact on academic and social functioning, along with its implications on the emergence of feelings of discomfort and anxiety. RESULTS Many psychopathological disorders are easily detectable and diagnosable in this particular stage of life, and although they are rooted in adolescent distress, they can generate real psychiatric disorders. CONCLUSIONS The data emerging from clinical practice stress the need for a comprehensive in order to identify possible subclinical symptoms for onset of mental illness, so as to be able to implement more targeted and less invasive therapeutic strategies.
Big and Open Linked Data (BOLD) in research, policy, and practice

Abstract The value of data as a new economic asset class is seldom realized on its own. With less reliance on self-administered survey, it offers new insights into behaviors and patterns. Yet, it involves a huge undertaking of bringing together multiple actors from different disciplines and diverse practices to examine the underexplored relationships between types of data. There are different inquiry systems and research cycles to make sense out of big and open data (BOLD). We argue that deploying theories from diverse disciplines, and considering using different inquiry systems and research cycles, offers a more disciplined and robust methodological approach. This allows us to break through the limits of backward induction from the evidence by moving back and forward in exploring the unknown through BOLD. As such, we call for developing a variety of rigorous approaches to counterbalance the current theory-free practice in the analysis and use of BOLD.
Repetitive nanosecond-pulse discharge in tip-grid gaps in atmospheric air

Recently, the generation of volume diffuse discharge under ultra-fast pulsed excitation attracted significant attention. In this paper, the discharge is generated in tip-grid gaps in air at atmospheric pressure using a solid-state pulse generator whose output pulse has a rise time of about 15ns and a full width at half maximum of 30–40ns. Both the characteristic and discharge mode are studied by the measurement of voltage-current waveforms and discharge images. The results show that the discharge during repetitive nanosecond-pulse excitation is inconsecutive, and the amplitude and shape of the measured discharge currents are not the same. The corresponding discharge images show that there are three discharge modes, i.e. diffuse, filamentary and diffuse — to — filamentary modes. In addition, the relationships between the discharge modes, applied voltage and the discharge current are obtained and discussed.
A Complete Solution of a Problem of Bondy Concerning Multipartite Tournaments

Ann-partite tournament is an orientation of a completen-partite graph. In 1976, Bondy raised the following problem: does every strongn-partite (n? 5) tournament, in which each partite set has at least two vertices, contain an (n+1)-cycle? It is the purpose of this paper to give a characterization of all suchn-partite tournaments without (n+1)-cycle.
SOD3 R231G polymorphism associated with coronary artery disease and myocardial infarction. The Ludwigshafen Risk and Cardiovascular Health (LURIC) study

This study examined the superoxide dismutase 3 (SOD3) R231G polymorphism in relation to the severity of coronary artery disease (CAD) and the risk of myocardial infarction (MI) in 3211 individuals; 94.4% of study participants were homozygous for SOD3 231RR and 5.5% were heterozygous for SOD3 231RG. The odds ratios of the RG and GG genotype (adjusted for age, gender and for conventional cardiovascular risk factors) were 2.02 (95% CI, 1.23–3.33, p=0.005) for the highest vs the lowest Friesinger coronary score and 1.40 (95% CI, 1.02–1.92, p=0.037) for MI, respectively. Further the SOD3 RG and GG genotype was associated with lower alpha-tocopherol levels than the wild type SOD3 RR genotype. It is concluded that the SOD3 231RG and GG genotype is associated with lower alpha-tocopherol levels and the severity of CAD and the risk of MI.
Structure modelling of the human body using FGMM

Human motion analysis and posture recognition have received increasing attention in the area of computer vision, for example, human-machine interaction. Structure modelling and analysis of human body is important for human motion analysis and posture recognition. In this paper, we propose a novel method based on fuzzy Gaussian mixture models (FGMM) to handle the task of structure modelling. To be more specific, Microsoft Kinect Sensor firstly captures raw 3D data of human body, followed by some data preprocessing steps, which include separating human body from the background and projection from 3D to 2D, etc. Then FGMM is applied to approximate the distribution of the processed 2D points. Experiments have been conducted to verify the effectiveness of proposed method on body structure modelling.
Vocalization based Individual Classification of Humpback Whales using Support Vector Machine

Visual surveys and DNA analysis hold primary importance in marine mammal observation. Especially, recognition of whales and their population density estimation rely heavily on these costly and tedious methods. Acoustic survey of vocalizing cetaceans, in contrast, is emerging as a promising technique for efficient, automatic, non-invasive and convenient observation and analysis of such vocally active organisms. However, it is, yet, too premature to replace the conventional methods altogether. In this paper, we have presented our results on recognition of individual humpback whales based on their vocalization data. Cepstral coefficients from song units extracted from audio records of seven humpback whales, re-sampled at 8KHz, were subjected to cepstral analysis. The extracted coefficients were used to develop multi-class support vector machine (SVM) classifier model. The test phase results indicated classification accuracy as high as 99% as compared to earlier best results of 88%, achieved by Gaussian mixture model (GMM) trained on cepstral coefficients. Furthermore, this improvement was attained with a highly reduced training dataset size. It was also verified that the song duration of approximately 128 seconds was sufficient for reliable identification in test phase for given dataset.
Fruit dry matter concentration: a new quality metric for apples.

BACKGROUND In the fresh apple market fruit must be crisp and juicy to attract buyers to purchase again. However, recent studies have shown that consumer acceptability could be further enhanced by improving taste. This study evaluates the use of fruit dry matter concentration (DMC) as a new fruit quality metric for apple. RESULTS Fruit samples collected at harvest, in the two main fruit growing regions of New Zealand, showed a variation in mean fruit DMC from 130 to 156 g kg(-1) with 'Royal Gala' and with 'Scifresh' from 152 to 176 g kg(-1). Individual fruit DMC showed a larger range, from 108 to 189 g kg(-1) with 'Royal Gala' and from 125 to 201 g kg(-1) with 'Scifresh'. Fruit DMC proved a more reliable predictor of total soluble solids after 12 weeks of air storage at 0.5 °C than TSS at harvest for both 'Royal Gala' and 'Scifresh'. Fruit DMC was also positively related to flesh firmness, although this relationship was not as strong as that seen with soluble solids and was more dependent on cultivar. Consumer studies showed that consumer preference was positively related to fruit DMC of 'Royal Gala' apples. CONCLUSION Fruit DMC can therefore be measured before or at harvest, and be used to predict the sensory potential for the fruit after storage.
On the Gap Between Restricted Isometry Properties and Sparse Recovery Conditions

We consider the problem of recovering sparse vectors from underdetermined linear measurements via <inline-formula> <tex-math notation="LaTeX">$\ell _{p}$ </tex-math></inline-formula>-constrained basis pursuit. Previous analyses of this problem based on generalized restricted isometry properties have suggested that two phenomena occur if <inline-formula> <tex-math notation="LaTeX">$p\neq 2$ </tex-math></inline-formula>. First, one may need substantially more than <inline-formula> <tex-math notation="LaTeX">$s \log (en/s)$ </tex-math></inline-formula> measurements (optimal for <inline-formula> <tex-math notation="LaTeX">$p=2$ </tex-math></inline-formula>) for uniform recovery of all <inline-formula> <tex-math notation="LaTeX">$s$ </tex-math></inline-formula>-sparse vectors. Second, the matrix that achieves recovery with the optimal number of measurements may not be Gaussian (as for <inline-formula> <tex-math notation="LaTeX">$p=2$ </tex-math></inline-formula>). We present a new, direct analysis, which shows that in fact neither of these phenomena occur. Via a suitable version of the null space property, we show that a standard Gaussian matrix provides <inline-formula> <tex-math notation="LaTeX">$\ell _{q}/\ell _{1}$ </tex-math></inline-formula>-recovery guarantees for <inline-formula> <tex-math notation="LaTeX">$\ell _{p}$ </tex-math></inline-formula>-constrained basis pursuit in the optimal measurement regime. Our result extends to several heavier-tailed measurement matrices. As an application, we show that one can obtain a consistent reconstruction from uniform scalar quantized measurements in the optimal measurement regime.
A Six-Oxidase Cascade for Tandem C-H Bond Activation Revealed by Reconstitution of Bicyclomycin Biosynthesis.

As a commercial antibiotic, bicyclomycin (BCM) is currently the only known natural product targeting the transcription termination factor rho. It belongs to a family of highly functionalized diketopiperazine (DKP) alkaloids and bears a unique O-bridged bicyclo[4.2. 2]piperazinedione ring system, a C1 triol, and terminal exo-methylene groups. We have identified and characterized the BCM biosynthetic pathway by heterologous biotransformations, in vitro biochemical assays, and one-pot enzymatic synthesis. A tRNA-dependent cyclodipeptide synthase guides the heterodimerization of leucine and isoleucine to afford the DKP precursor; subsequently, six redox enzymes, including five α-ketoglutarate/Fe2+ -dependent dioxygenases and one cytochrome P450 monooxygenase, regio- and stereoselectively install four hydroxy groups (primary, secondary, and two tertiary), an exo-methylene moiety, and a medium-sized bridged ring through the functionalization of eight unactivated C-H bonds.
Synthesis and optimization of high-level stream programs

In this paper we address the problem of translating high-level stream programs, such as those written in MPEG's RVC-CAL dataflow language, into implementations in programmable hardware. Our focus is on two aspects: sufficient language coverage to make synthesis available for a large class of programs, and methodology and tool support providing analysis and guidance to improve and optimize an initial implementation. Our main results are (1) a synthesis tool that for the first time translates a complete and unmodified MPEG reference implementation into a working hardware description, and (2) a suite of profiling and analysis tools that analyze the structure of computation weighted by data obtained from the synthesis process, and accurately pinpoint parts of the program that are targets for optimization.
Differential responsiveness among “high risk” pediatric brain tumors in a pilot study of dose‐intensive induction chemotherapy

These factors have been predictive for progressive disease on therapy (PDOT) among pediatric brain tumors: >1.5 cm2 unresectable tumor, glioblastoma, supratentorial primitive neuroectodermal tumor, and metastatic medulloblastoma (MBL). This pilot study sought to correlate cytoreductive response with progression free survival.
The need for close monitoring of early psychosis and co-occurring substance misuse

Substance misuse is widespread among individuals with early-phase psychotic disorders and is associated with a worse illness course. Thorough assessment of patterns of substance misuse at admission for psychiatric care is often lacking and can compromise the accuracy of a diagnostic assessment that distinguishes between a primary psychosis and one that is substance induced. Given the risk to recovery from psychosis posed by substance misuse, close monitoring of the course and treatment of early-phase psychosis that is accompanied by substance misuse is indicated and could inform the development of more effective dual-diagnosis treatments.
An experiment and preliminary results for narrow-and wideband land mobile satellite propagation at K-band

With increasing utilization of satellite mobile services capacity shortage in respectively common frequency bands (L band) is to be expected. To enhance understanding of propagation channel characteristics at higher frequency bands (Ku/Ka band) an experiment with a mobile receiver, using narrow- and wideband test signals has been carried out. An airborne test platform has allowed to consider real satellite incidence angles. A comprehensive database has been obtained on channel behaviour and — by means of auxiliary instruments -documenting the details of four typical scenarios measured. Preliminary results indicate excellent qualitative plausibility, such as recognition of electricity poles. Ongoing quantitative analyses include comparisons to literature, e.g. specific attenuation in vegetation. Focus is a statistical analysis to set up a channel simulator predicting the propagation channel characteristics under the measured scenarios' conditions. It is planned to submit results for standardization.
Soluble fms-like tyrosine kinase-1 antibody for diagnosis purposes (WO2010075475)

Background: The application (WO2010075475) is involved in the diagnosis of angiogenesis-dependent diseases related with the soluble FMS-like tyrosine kinase-1 (sFlt-1) biomarker. Objective: It aims to identify, characterize and produce antibodies raised against sFlt-1 for the diagnosis of preeclampsia as well as other cardiovascular diseases. Methods: The diagnostic kit is based on a double mAb sandwich assay, comprising of a capture anti-sFlt-1 mAb, grafted onto paramagnetic particles that are able to bind sFlt-1 both in bound and free forms. An acridinium conjugated anti-sFlt-1 antibody, which binds to s-Flt1 on another epitope, serves as a chemioluminescent label. Results: Using this assay, the total amount of sFlt-1 could be estimated in various biological samples. Conclusion: Antibodies against the free and bound forms of sFlt-1 offer new opportunities in the diagnostics of preeclampsia and other angiogenesis-dependent disorders. Furthermore, as demonstrated in this patent, the immunoassay could be automated and is fast and reliable.
A nonsubstituted primary hydroxyl group in position 6' of free lipid A is required for binding of lipid A monoclonal antibodies

Lipid A monoclonal antibodies, which require for binding the presence of the bisphosphorylated D-glucosamine disaccharide lipid A backbone, were tested against synthetic lipid A precursor Ia and compound B 1047 by enzyme immunoassay. The last-named compound is a precursor Ia analog with a methoxy instead of a hydroxy group at C6' and was chosen to determine why these antibodies failed to recognize the bound lipid A present in lipopolysaccharide (LPS). Whereas all antibodies tested bound to precursor Ia, none of them bound to compound B 1047 or Escherichia coli Re LPS to a significant extent. Compared to the natural substituent at C6', i.e., 3-deoxy-D-manno-octulosonic acid (Kdo), the methoxy group is neither bulky nor charged. Thus, the data suggest that it is not hindrance by Kdo but rather the generation of a neoantigen that endows lipid A with immunoreactivity upon liberation from LPS by acid hydrolysis.
Enhanced Ultraviolet Response of  ${\rm p}\hbox{-}{\rm Si}/{\rm SiO}_{x}/{\rm i}\hbox{-}{\rm ZnO}/{\rm n}\hbox{-}{\rm ZnO}$  Photodetectors

Ultraviolet (UV) response of p-Si/i-ZnO/n-ZnO (p-i-n) heterojunction photodetectors (HPDs) was dramatically enhanced by inserting a thin SiOx layer to form a p-Si/SiOx/ i-ZnO/n-ZnO structure. It was found that the leakage current of p-i-n HPDs is largely reduced by about three orders compared with that of conventional p-n HPDs. Photocurrent measurements show that the UV-generated carriers are first accelerated by the electric field in i-ZnO and then tunnel through the thin SiOx layer instead of being trapped by the interface states at the p-Si/ i-ZnO interface. This increases the UV response and consequently, the UV-to-visible rejection ratio is enhanced from 7 for the p-i-n HPDs without SiOx layer to 112 for the HPDs with SiOx insertion.
Vascular endothelial growth factor-A-induced chemotaxis of monocytes is attenuated in patients with diabetes mellitus: A potential predictor for the individual capacity to develop collaterals.

BACKGROUND Vascular endothelial growth factor-A (VEGF-A) acts on endothelial cells and monocytes, 2 cell types that participate in the angiogenic and arteriogenic process in vivo. Thus far, it has not been possible to identify differences in individual responses to VEGF-A stimulation because of the lack of an ex vivo assay. METHODS AND RESULTS We report a chemotaxis assay using isolated monocytes from individual diabetic patients and from healthy, age-matched volunteers. The chemotactic response of individual monocyte preparations to VEGF-A, as mediated via Flt-1, was quantitatively assessed using a modified Boyden chamber. Although the migration of monocytes from healthy volunteers could be stimulated with VEGF-A (1 ng/mL) to a median of 148.4% of the control value (25th and 75th percentiles, 136% and 170%), monocytes from diabetic patients could not be stimulated with VEGF-A (median, 91.1% of unstimulated controls; 25th and 75th percentiles, 83% and 98%; P<0.0001). In contrast, the response of monocytes to the chemoattractant formylMetLeuPhe remained intact in diabetic patients. The VEGF-A-inducible kinase activity of Flt-1, as assessed by in vitro kinase assays, remained intact in monocytes from diabetic patients. Moreover, the serum level of VEGF-A, as assessed by immunoradiometric assay, was significantly elevated in diabetic patients. CONCLUSIONS The cellular response of monocytes to VEGF-A is attenuated in diabetic patients because of a downstream signal transduction defect. These data suggest that monocytes are important in arteriogenesis and that their ability to migrate might be critical to the arteriogenic response. Thus, we resolved a fundamental mechanism involved in the problem of impaired collateral formation in diabetic patients.
Value of a shortened questionnaire in the description of asthma in 10–12‐year‐old pupils

Asthma is a common and severe chronic disease in children influencing their quality of life and functioning at school. A 5‐item asthma‐screening instrument was developed and tested in 1052 children aged 10–12 years. Questionnaires were completed by parents and children separately and data were compared. Children reported less to be diagnosed by a medical doctor as having asthma compared with their parents, although children reported more to have certain asthma symptoms. No difference in prevalence of asthma was found between children and parents’ answers. The absolute agreement for the scale was 92% and a good kappa agreement was found. Recoding the ‘don't know’‐answers in ‘no’‐answers resulted in a 4% misclassification. The short 5‐item asthma screening tool can be valuable in the categorization of a subgroup of children likely to suffer from asthma in a survey. Recoding ‘don't know’‐answers to ‘no’‐answers is justified in large samples.