Miracle-dz/UniFAR-FTUs huggingface dataset:
Enzymatic construction of shRNA library from oligonucleotide library

Background Short hairpin RNAs (shRNAs) expressed from vectors have been used as an effective means of exploiting the RNA interference (RNAi) pathway in mammalian cells. Genome-scale screening with shRNA libraries has been used to investigate the relationship between genotypes and phenotypes on a large scale. Although several methods have been developed to construct shRNA libraries, their broad application has been limited by the high cost of constructing these libraries. Objective We develop a new method that efficiently constructs a shRNA library at low cost, using treatments with several enzymes and an oligonucleotide library. Methods The library of shRNA expression cassettes, which were cloned into a lentiviral plasmid, was produced through several enzymatic reactions, starting from a library of 20,000 different short oligonucleotides produced by microarray-based oligonucleotide synthesis. Results The NGS sequence analysis of the library shows that 99.8% of them (19,956 from 20,000 sequences) were contained in the library: 63.2% of them represent the correct sequences and the rest showed one or two base pair differences from the expected sequences. Conclusion Considering the ease of our method, shRNA libraries of new genomes and of specific populations of genes can be prepared in a short period of time for genome-scale RNAi library screening.
Systemic Corticosteroids during Acute Exacerbations of Chronic Obstructive Pulmonary Disease ( COPD ) ?

The employment of systemic corticosteroids in the treatment of acute exacerbations of chronic obstructive pulmonary disease (COPD) has been shown to improve airway limitation, decrease treatment failure and risk of relapse, and may improve symptoms in addition to decreasing the length of hospital stay. Nowadays, all clinical guidelines recommend systemic corticosteroids to treat moderate or severe COPD exacerbations. However, their use is associated with potential side effects, mainly hyperglycemia. In the era of precision medicine, the possibility of employing blood eosinophil count has emerged as a potential way of optimizing therapy. Issues regarding the intra-individual variability of blood eosinophil count determination, a lack of clear data regarding the real prevalence of eosinophilic acute exacerbations, the fact that previously published studies have demonstrated the benefit of systemic corticosteroids irrespective of eosinophil levels, and especially the fact that there is only one well-designed study justifying this approach have led us to think that we are not ready to use eosinophil count to guide treatment with systemic corticosteroids during acute exacerbations of COPD.
Estado nutricional de crianças do baixo Amazonas : concordância entre três critérios de classificação Nutritional status of children from lower Amazonas : agreement between three classification criteria

Método: Estudo exploratório, epidemiológico com desenho transversal de base escolar realizado em 2014 com crianças do Ensino Infantil da cidade de Parintins. Foi avaliado o estado nutricional, pelo índice de massa corporal, segundo três critérios de avaliação: Conde e Monteiro, Cole et al. e Organização Mundial da Saúde (OMS). Aplicaram-se testes descritivos e de associação para análise do comportamento das variáveis e o coeficiente de Kappa para investigar a concordância dos resultados entre os três critérios.
Cardiac rehabilitation after myocardial infarction: a comparison between the standard and home-based cardiac rehabilitation programs

Cardiovascular diseases (CVD) represent the leading cause of death in the world, accounting for 30% of global deaths.1,2 Hospitalbased cardiac rehabilitation (CR) is well-established in the effective management of patients with acute coronary syndrome (ACS). CR has been associated with improved survival, quality of life, functional status and cardiovascular risk profile as well as reduction in hospital re-admission.3 CR is an evidence-based form of secondary prevention; where exercise-based CR is currently a class I indication in clinical practice guidelines, including those for ST segment elevation MI4,5 however; referral is suboptimal and participation rates are estimated at 10–30%6 with lowest participation in the elderly, females, ethnic groups, and low-income populations. Barriers limiting participation in traditional CR programs include cost, transport diffculties, busy work schedules, lack of perceived need and low referral rates by the
Diagnosis andmanagementofmeningococcal disease : theneed for centralized care

Meningococcal infection remains a significant health problem in children, with a significant mortality and morbidity. Prompt recognition and aggressive early treatment are the only effective measures against invasive disease. This requires immediate administration of antibiotic therapy, and the recognition and treatment of patients who may have complications of meningococcal infection such as shock, raised intracranial pressure (ICP) or both. Encouragingly, its mortality has fallen in recent years. This is the result of several factors such as the centralization of care of seriously ill children in paediatric intensive care units (PICUs), the establishment of specialized mobile intensive care teams, the development of protocols for the treatment of meningococcal infection, and the dissemination by national bodies and charities of guidance about early recognition and management. We will review the pathophysiology and management of the different presentations of meningococcal disease and examine the possible role of adjunctive therapies.
6298 Employer-Paid Parking , Mode Choice , and Suburbanization Abstract

This paper constructs a theoretical model that facilitates analysis of the effects of employer-paid parking on mode choice, road investment and suburbanization. The model simplifies urban space by dividing it into two zones (islands), center and suburbs, which are connected by a congested road and a public-transit line. Each road commuter requires an allotment of CBD land for parking, and because the central zone’s area is fixed, parking land reduces the amount available for central residences and CBD production. The model characterizes optimal resource allocation from the perspective of a social planner. The planning solution can be decentralized, which requires employeerather than employer-paid parking, congestion tolls, and a tax (subsidy) to offset the road capacity deficit (surplus). The analysis then considers the effect of switching to employer-paid parking, with the burden of parking costs shifting from road users to employers, thus reducing the wage for all workers. This switch inefficiently increases road usage and capacity investment, while spurring an inefficient increase in suburbanization. JEL-Codes: R400.
Salt-tolerant rootstock increases yield and photosynthesis of pepper under salinity through maintenance of root strength and proline accumulation

The performance of a salt-tolerant pepper (Capsicum annuum L.) accession (A25) utilized as a rootstock was assessed in two experiments. In a first field experiment under natural salinity conditions, we observed a larger amount of marketable fruit (+75%) and lower Blossom end Root incidence (-31%) in commercial pepper cultivar Adige (A) grafted with A25 (A/A25) compared with ungrafted plants. In order to understand this behavior a second greenhouse experiment was conducted to determine growth, mineral partitioning, gas exchange and chlorophyll a fluorescence parameters, antioxidant systems and proline content in A and A/A25 plants under salinity conditions (80 mM NaCl for 14 days). Salt stress induced significantly stunted growth of A plants (-40.6% of leaf dry weight) compared to the control conditions, while no alterations were observed in A/A25 at the end of the experiment. Accumulation of Na and Cl in leaves and roots was similar in either grafted or ungrafted plants. Despite the activation of protective mechanisms (increment of superoxide dismutase, catalase, ascorbate peroxidase activity and non-photochemical quenching), A plants showed severely reduced photosynthetic CO2 assimilation (-45.6% of AN390) and substantial buildup of malondialdehyde (MDA) by-product, suggesting the inability to counteract salt-triggered damage. In contrast, A/A25 plants, which had a constitutive enhanced root apparatus and 2.6-fold higher proline content under salinity, did not show alterations in photosynthesis and growth and MDA levels increased only slightly. Our results underline that salt tolerance in A/A25 grafted plants could be mediate by (I) the maintenance of root sink strength and (II) the markedly increased proline levels that could balance cell osmotic pressure thus protecting enzymatic stability from salttriggered damage.
Title State-dependent divergences in the entanglement entropy

We show the entanglement entropy in certain quantum field theories to contain state-dependent divergences. Both perturbative and holographic examples are exhibited. However, quantities such as the relative entropy and the generalized entropy of black holes remain finite, due to cancellation of divergences. We classify all possible state-dependent divergences that can appear in both perturbatively renormalizeable and holographic covariant d ≤ 6 quantum field theories. marolf@physics.ucsb. edu, aroncwall@gmail.com ar X iv :1 60 7. 01 24 6v 1 [ he pth ] 5 J ul 2 01 6
Highlights on a Multiobjective Routing Method for Multiservice MPLS Networks with Traffic Splitting

A multiobjective routing model for Multiprotocol Label Switching networks with multiple service classes and considering traffic splitting is presented. The routing problem is formulated as a multiobjective mixed-integer program, and an exact resolution method based on the classical constraint method is outlined. Some experimental results on network performance measures, resulting from the application of the routing method in a reference test network, are presented. These results confirm the potential advantages of using a multiobjective optimization model in this routing problem, as we get a compromise solution that tries to balance the two considered objectives. Keywords—Routing models; Multiobjective optimization; Telecommunication networks; Network flow approach; Traffic splitting.
Quantitative analysis of alterations in liver ultrastructure of rainbow trout Oncorhynchus mykiss after administration of the aquaculture antibacterials oxolinic acid and flumequine

The 4-quinolone antibacterials oxolinic acid and flumequine are routinely and successfully used in commercial aquaculture. In order to investigate the effects of these antibiotics on fish liver ultrastructure, rainbow trout were fed diets containing oxolinic acid or flumequine at concentrations of 1.0 and 1.2 g kg-' food, respect~vely, at a ration of 1 % body weight d-' for 10 d. F ~ s h were sampled for examination on Days 1 and 10 after a return to normal diet and hepatic ultrastructural alterations were quantified by stereological analysis. Both drugs induced an almost 2.5-fold increase in the volume of the rough endoplasmic reticulum, which displayed phenomena of dilation, vesiculation and fragmentation. Glycogen deposits were depleted to approximately 50 % of their control volume accompanied by the formation of clusters of lipid droplets. Additional structural alterations included the intralysosomal accumulation of stacked membranes and the presence of elaborated myelin figures interspersed in the cytoplasm. No drug-specific responses were observed. In general, compensatory rather than degenerative changes followed the antibiotic administration. Further signs supporting a stimulation in the hepatocellular metabolic activity were mitochondrial hyperplasia after flumequine administration and high nuclear-cytoplasmic ratios in individuals treated with oxolinic acid.
Spatial Statistics 2011 Incorporating Spatial Variability Measures in Landcover Classification using Random Forest

The spatial variability of remotely sensed image values provides important information about the arrangement of objects and their spatial relationships within the image. The characterisation of spatial variability in such images, for example, to measure of texture, is of great utility for the discrimination of land cover classes. To this end, the variogram, a function commonly applied in geostatistics, has been used widely to extract image texture for remotely sensed data classification. The aim of this study was to assess the increase in accuracy that can be achieved by incorporating univariate and multivariate textural measures of Landsat TM imagery in classification models applied to large heterogeneous landscapes. Such landscapes which difficult to classify due to the large number of land cover categories and low inter-class separability. Madogram, rodogram and direct variogram for the univariate case, and crossand pseudocross variograms for the multivariate one, together with multi-seasonal spectral information were used in a Random Forest classifier to map land cover types. The addition of spatial variability into multi-seasonal Random Forest models leads to an increase in the overall accuracy of 8%, and to an increase in the Kappa index of 9%, respectively. The increase in per categories Kappa for the textural Random Forest model reached 30% for certain categories. This study demonstrates that the use of information on spatial variability produces a fundamental increase in per class classification accuracy of complex land-cover categories. © 2010 Published by Elsevier Ltd. Selection and/or peer-review under responsibility of [name organizer] "
Relationships Between Care Management Results by Public Health Nurses and Psychological Factors of Care Givers

The purpose of this study was performed to investigate the relationships between the users' evaluation of the care management by public health nurses and. the psychological factors of the care givers. The subjects of the study were care givers for whom care management was provided by public health nurses. The' subjects (care givers) were requested to answer a questionnaire sent by mail. The questionnaire was collected from 413 of 621 subjects (return rate: 66.5%). Among the 413 responses, 356 were usable for data analyses. The results were that the care givers with higher self-efficacy, the care givers tended to feel that "the care management service was introduced and modified in a user-oriented manner," "reliable talk and actions were always available," and "the care manager service allowed us to live independent lives in our own way. " And the care giv ers who obtained higher subjective satisfaction were more likely to feel that "the care manager service allowed us to live independent lives in our own way. " It will be necessary to more deeply understand the care users' psychological charac teristics related with care management to provide more specialized care manage ment.
Heterogeneity in earnings quality between different classes of companies after IFRS adoption : evidence from Brazil

This paper aims to investigate the existence of heterogeneity in earnings quality between different classes of companies after the adoption of the International Financial Reporting Standards (IFRS). IFRS adoption is generally associated with an increase in the quality of financial statements. However, companies within the same country are likely to have different economic incentives regarding the disclosure of information. Thus, treating companies equally, without considering the related economic incentives, could contaminate earnings quality investigations. The case of Brazil is analyzed, which is a country classified as code-law, in which tax laws determined accounting practice and in which IFRS adoption is mandatory. First, Brazilian companies listed on the São Paulo Stock, Commodities, and Futures Exchange (BM&FBOVESPA) were separated into two classes: companies issuing American Depositary Receipts (ADRs) before IFRS adoption and companies that did not issue ADRs until the adoption of IFRS. Then, this second class of companies was grouped, using cluster analysis, into two different subclasses according to economic incentives. Based on the groups identified, the quality of accounting earnings is tested for each class of the companies before and after IFRS adoption. This paper uses timely recognition of economic events, value relevance of net income, and earnings management as proxies for the quality of accounting earnings. The results indicate that a particular class of companies began showing conditional conservatism, value relevance of net income, and lower earnings management after IFRS adoption. On the other hand, these results were not found for the two other classes of companies.
eview ormones in synergy : Regulation of the pituitary gonadotropin genes

The precise interplay of hormonal influences that governs gonadotropin hormone production by the pituitary includes endocrine, paracrine and autocrine actions of hypothalamic gonadotropin-releasing hormone (GnRH), activin and steroids. However, most studies of hormonal regulation of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in the pituitary gonadotrope have been limited to analyses of the isolated actions of individual hormones. LH and FSH subunits have distinct pateywords:
End-to-end training of deep probabilistic CCA for joint modeling of paired biomedical observations

Many diseases are diagnosed by pathologists using key morphological features in imaging data; but the genes that capture the internal state of cells and are therefore associated with specific morphologies are typically unknown. To address this question, the GTEx Consortium [8; 7] has collected data from over 948 autopsy research subjects, including standardized whole tissue slides and RNA sequencing gene expression levels from approximately 50 different human tissues. These multi-subject, multiview data provide an opportunity to develop computational tools that quantify how genome-level associations affect morphological features observable in histology slides.
The effect of conservatism on accrual and stock return

According to representation theory, hope of future good news stimulates the managers to postepone bad economic news in order to gain extra return. But in fact conservatism in accounting, as an influential control mechanism in identifying the good versus bad economic news, prevent the managers from over-optimism and provides a framework for identifying economic affairs. Therefore, it is the intention of this research to study the aforementioned challenge through analyzing the relation between conservatism, accrual persistence asymmetry and stock return. Conservatism is considered as independent variable of the research and accrual persistence asymmetry and stock return as dependent variables. Moreover, Basu’s model its used for measuring conservatism and to control risk, stock return is moderated in extent. Admitted firms in Tehran’s Bond Exchange from 2009 to 2015 constitude the date of the present research from which a sum of 66 firms (462 year-firm) are placed within the sample data of this research. The assumption of the research are tested via analyzing coefficient variables? regression modeling findings of the research suggest that there exists no meaningful relation between resistance of accrual items and stock return and another analysis rejected the existence of such relation has been rejected which suggested that law makings and standards are weak in a way that provide the ground for manipulating the real activities altimately leading to increased operational risks.
The Sustainable Development of Oued Noun Oases through the Integration in the Biosphere Reserve Oasis of Southern Morocco

The Moroccan government has adopted a new approach to development that integrates environmental, renewal of natural resources, measures for the reduction of poverty at the social and spatial distribution of wealth at an advanced regionalization plan. In 2000, UNESCO in collaboration with the Moroccan State creates Biosphere Reserve Oasis of Southern Morocco (BROSM) in the Provinces of "Errachidia, Ouarzazate and Zagora" to support the strategies of preserving the environment. The project to integrate the Oasis of Oued Noun in the program of the biosphere reserve Oasis of southern Morocco (BROSM) will have a positive impact on the region. This direction aims to strengthen the efforts of the government and ONG programs working in the sector. Through this institutional status, it becomes appropriate to rethink about the preservation and enhancement of the natural heritage and local culture.
Conducting online research and evaluation.

Web-based evaluation/surveys and research about the Internet are becoming increasingly widespread. The apparent ease of conducting these activities may be blinding researchers to potential methodological flaws that threaten the validity of the data and any interpretations that may be drawn from them. Additionally, some online research and survey methods threaten the central tenets of human research: privacy, confidentiality and informed consent. This paper outlines three case studies at Griffith University as a point of departure for discussing these emerging issues.
Understanding cardiac electrical phenotypes in the genomic era

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Robust Optimal Control with Adjustable Uncertainty Sets 1

In this paper, we develop a unified framework for studying constrained robust optimal control problems with adjustable uncertainty sets. In contrast to standard constrained robust optimal control problems with known uncertainty sets, we treat the uncertainty sets in our problems as additional decision variables. In particular, given a finite prediction horizon and a metric for adjusting the uncertainty sets, we address the question of determining the optimal size and shape of the uncertainty sets, while simultaneously ensuring the existence of a control policy that will keep the system within its constraints for all possible disturbance realizations inside the adjusted uncertainty set. Since our problem subsumes the classical constrained robust optimal control design problem, it is computationally intractable in general. We demonstrate that by restricting the families of admissible uncertainty sets and control policies, the problem can be formulated as a tractable convex optimization problem. We show that our framework captures several families of (convex) uncertainty sets of practical interest, and illustrate our approach on a demand response problem of providing control reserves for a power system.