Miracle-dz/UniFAR-FTUs huggingface dataset:
Autophagosomal IκBα Degradation Plays a Role in the Long Term Control of Tumor Necrosis Factor-α-induced Nuclear Factor-κB (NF-κB) Activity*

Transcription factor NF-κB is persistently activated in many chronic inflammatory diseases and cancers. The short term regulation of NF-κB is well understood, but little is known about the mechanisms of its long term activation. We studied the effect of a single application of TNF-α on NF-κB activity for up to 48 h in intestinal epithelial cells. Results show that NF-κB remained persistently activated up to 48 h after TNF-α and that the long term activation of NF-κB was accompanied by a biphasic degradation of IκBα. The first phase of IκBα degradation was proteasome-dependent, but the second was not. Further investigation showed that TNF-α stimulated formation of autophagosomes in intestinal epithelial cells and that IκBα co-localized with autophagosomal vesicles. Pharmacological or genetic blockade of autophagosome formation or the inhibition of lysosomal proteases decreased TNF-α-induced degradation of IκBα and lowered NF-κB target gene expression. Together, these findings indicate a role of autophagy in the control of long term NF-κB activity. Because abnormalities in autophagy have been linked to ineffective innate immunity, we propose that alterations in NF-κB may mediate this effect.
Estimates of the extent of morbidity and mortality due to occupational diseases in Canada.

OBJECTIVE To estimate the extent of occupational disease morbidity and mortality in Canada by comparing and contrasting four different data sources. DATA SOURCES 1) Canadian National Workers' Compensation Boards Statistics, 2) U.S. Bureau of Labor Statistics adjusted to Canadian Workforce, 3) California Physician's First Reports adjusted to the Canadian Workforce, and 4) proportionate model of overall disease incidence obtained through literature review. MAIN FINDINGS Each data source was limited in its ability to provide a true estimate of the extent of morbidity and mortality due to occupational disease in Canada. Collectively, an estimate of between 77,900 and 112,000 new cases of occupational diseases and 2,381 to 6,010 occupational disease deaths were obtained. CONCLUSIONS Occupational diseases are a significant and under-estimated cause of morbidity and mortality in Canada.
ERP Indices and Neuropsychological Performance as Predictors of Functional Outcome in Dementia

We compared the relative value of neuropsychological and event-related potentials (ERPs) obtained during both passive and active auditory oddball paradigm measures for determining functional outcome in dementia 4 years following initial assessment. Functional outcome was assessed by structured interview of family members of 29 patients with dementia, and patients' functional status was rated in seven areas: mortality, incontinence, institutionalization, ADL dependence, verbal responsiveness, recognition of family members, and capacity for social interaction. A total functional outcome score (ADLTOTAL) was obtained by summing across these individual outcome measures. Many of the neuropsychological measures correlated strongly with overall functional outcome, whereas P3 amplitude and latency on the active ERP condition were the only ERP indices to predict functional outcome. When ERP and neuropsychological measures were considered simultaneously using stepwise multiple regression analyses, the neuropsychological measures were better predictors of most functional outcomes, although P3 latency was the best predictor of mortality. However, neuropsychological performance and ERPs appear to be sensitive to different functional outcomes. Therefore, evaluation of both ERPs and neuropsychological performance may ultimately have prognostic utility in the assessment of patients with dementia.
Incidence of antimicrobial-resistant ventilator associated pneumonia: an eighteen-month survey.

AIM Microbes commonly involved in ventilator associated pneumonia (VAP) are difficult to eradicate: Pseudomonas aeruginosa and Acinetobacter baumannii are often resistant to piperacillin, aztreonam and ceftazidim while Klebsiella pneumoniae and other Enterobacteriaceae are resistant to third generation cephalosporins. Physicians need to tailor their therapeutic approach depending on individual patients and clinical setting, firmly based on local epidemiology. The aim of this study was to assess the incidence of VAP caused by multidrug-resistant bacteria in our ICU. METHODS We conducted a retrospective review of all patients mechanically ventilated for longer than 72 h, who developed pneumonia over an eighteen-month period for whom final culture data were available. One-hundred and forty-three patients admitted to a University Hospital medical surgical ICU were included in this survey. VAP was diagnosed by Centers for Disease Control and Prevention criteria and confirmed by mini- bronchoalveolar lavage. RESULTS In this study, the incidence of VAP in the period January 1, 2002-June 31, 2003 was very high, accounting for about 20% of all at-risk cases. Multiresistant agents were involved in about 57% of VAP occurring within 7 days of mechanical ventilation, and in 80% of late VAP. CONCLUSIONS This study revealed differences in VAP incidence and features among our ICU and literature data, indicating a need for caution when using hospital infection surveillance data for comparisons and choice of therapy. In our setting, with high levels of antibiotic resistance, combined approaches of non-antibiotic using strategies and education programs might be beneficial.
Decline in spindle support to alpha‐motoneurones during sustained voluntary contractions.

1. To address whether the muscle spindle support to alpha‐motoneurones is maintained during prolonged isometric voluntary contractions, the discharge of eighteen muscle spindle afferents, originating in the dorsiflexors of the ankle or toes, was recorded from the common peroneal nerve in eight subjects. Isometric contractions were generally sustained for 1 min, usually below 30% of the maximal voluntary dorsiflexion force. 2. Once the afferent had been identified, subjects were instructed to dorsiflex the foot slowly to recruit the spindle ending, to continue the ramp contraction until a predetermined target force was reached, and then to hold that force until requested to relax. 3. Five muscle spindle afferents maintained a constant discharge frequency during the hold phase of the isometric contraction. Following relaxation of the contraction two spindle afferents from tibialis anterior, exhibited a post‐contraction discharge despite the absence of detectable electromyographic activity (EMG). 4. The discharge frequency of most of the spindle afferents (72%) declined progressively during the isometric contraction. The mean firing rates had declined to two‐thirds of those at the onset of the contraction by 30 s, and to half after 1 min. The decline in spindle firing rate commenced during the ramp phase of the contraction and was statistically significant by 10 s, when force was held constant. The extent of the decline was greater for those units with the higher initial firing rates and for those units studied after many preceding contractions. 5. In the same contractions a progressive increase in EMG was required to maintain force and consequently the change in EMG was inversely related to the change in spindle discharge. While many mechanisms may contribute to the decline in spindle discharge during a sustained isometric contraction, it is argued that the result will be a progressive disfacilitation of alpha‐motoneurones, which may contribute to the decline in motor unit firing rates during a sustained contraction.
Online estimation of induction motor parameters using a modified particle swarm optimization technique

This paper addresses an application of Particle Swarm Optimization algorithm to dynamically estimate and track the changes in parameters of an induction Motor in steady-state condition. In real-time operation, the performance of the control system is influenced by various environmental and internal factors. The former studies used the data in offline condition of the motor to estimate the parameters. In this novel method however, the measured three-phase currents, voltages and the speed of the induction machine are used as inputs and the effect of the temperature rise on the motor parameters; i.e., rotor and stator resistances is investigated implementing a two-stage single-flock particle swarm optimization technique. The simulation is based on a proper model of the induction motor, including electromagnetic and mechanical elements. The parameters are calculated and the estimation errors are minimized via a normalized root mean square error measure respectively.
ROLE OF CORPORATE SOCIAL RESPONSIBILITY IN THE FINANCIAL PERFORMANCE OF HORMUUD TELECOM-MOGADISHU-SOMALIA

The field of corporate social responsibility (CSR) has grown exponentially in the last decade. Nevertheless, there remains a protracted debate about the legitimacy and value of corporate responses to CSR concerns. There are different views of the role of the firm in society and disagreement as to whether wealth maximization should be the sole goal of a corporation. The relationship is tested by using empirical methods. The Corporate Social Responsibilities of Hormuud Telecom goes far beyond the old model of philanthropy of donating money for a good cause at the end of the financial year. The main objective of the study was to find out the role of Corporate Social Responsibilities on the financial performance. The research design followed was descriptive and a survey study was adopted. A sample of 316 out of over 1500 employees of the Hormuud Telecommunication were interviewed. These were accessed by use of questionnaires regarding the variables of financial performance factors. The obtained data from the survey was analyzed using SPSS. From the analysis, it was found out that, Social Networking (SNW) play a major role in organizational financial performance through increased revenue, customer loyalty and profits. Philanthropic activities were also agreed that they are relevant to financial performance. From the regression analysis prediction, philanthropic activities would negatively contribute to a -. 297 unit per activity, and lastly physical environment is to be taken care of but its contribution to financial performance was relatively low to their counterpart variables. The study concluded that, CSR activities should be supported by organizations in order for them to realize the earmarked financial benefits but also to avoid government penalties in fines. The study recommends that, organizations should uphold CSR activities including all SNW, philanthropy and Physical environment support. The study also recommends that further studies be done on the subject of CSR and its role on financial performance of organization and to take this study to other entities as well. Key Terms: Corporate Social Networking, Corporate Social Philanthropy, Corporate Social Environment
The Mg2+ block and intrinsic gating underlying inward rectification of the K+ current in guinea‐pig cardiac myocytes.

1. The blockade by Mg2+ and intrinsic gating of the channel, which underlie the rectification of the inward rectifier K+ current, was investigated using the oil‐gap voltage clamp method in isolated guinea‐pig ventricular cells. 2. The inward rectifier K+ current was isolated by subtracting trans‐gap currents recorded at an extracellular K+ concentration ([K+]o) of 0 mM from those obtained at 14 mM [K+]o in the presence of a given concentration of intracellular Mg2+ ([Mg2+]i). The reversal potential (V0) of the difference current was near the equilibrium potential for K+ (EK). 3. On repolarization across EK, the inward rectifier K+ current showed a rapid exponential increase. The time constant decreased with increasing hyperpolarization, but it was independent of both [Mg2+]i and the preceding depolarization. 4. When the pre‐pulse potential was made progressively positive between V0‐20 and V0 + 30 mV, the amplitude of the time‐dependent component became larger and the preceding current jump decreased at any [Mg2+]i. With pre‐pulses more positive than V0 + 40 mV, the time‐dependent component started from almost the zero current level at 2 microM [Mg2+]i. At higher [Mg2+]i (350, 500 and 3000 microM), however, the time‐dependent component became smaller as the pre‐pulse potential was made more positive than V0 + 40 mV. 5. When the membrane was depolarized from a potential of full activation at 2 microM [Mg2+]i, the initial jump in the outward current was ohmic and was followed by an exponential decay. The time‐dependent component of the inward current, recorded on repolarization after increasing durations of the preceding depolarization, developed as the outward current decayed. The time constants of both processes were in good agreement. 6. At 500 microM [Mg2+]i, the outward current on depolarization was instantaneously rectified. The time‐dependent component recorded on repolarization developed with prolongation of the pre‐pulse with a time course slower than at 2 microM [Mg2+]i. The envelope time course became slower as the potential of the depolarization became more positive. 7. Lowering the temperature from 23 to 15 degrees C slowed the time‐dependent current with an apparent Q10 of about 3.5 at V0. 8. Based on the experimental data, kinetic parameters were estimated for a model of Mg2+ block, which well simulated the inward‐going rectification of the K+ current. 9. It is concluded that the instantaneous inward rectification on depolarization is due to the Mg2+ block at physiological [Mg2+]i. (ABSTRACT TRUNCATED AT 400 WORDS)
Behavioral and Single-Neuron Sensitivity to Millisecond Variations in Temporally Patterned Communication Signals

In many sensory pathways, central neurons serve as temporal filters for timing patterns in communication signals. However, how a population of neurons with diverse temporal filtering properties codes for natural variation in communication signals is unknown. Here we addressed this question in the weakly electric fish Brienomyrus brachyistius, which varies the time intervals between successive electric organ discharges to communicate. These fish produce an individually stereotyped signal called a scallop, which consists of a distinctive temporal pattern of ∼8–12 electric pulses. We manipulated the temporal structure of natural scallops during behavioral playback and in vivo electrophysiology experiments to probe the temporal sensitivity of scallop encoding and recognition. We found that presenting time-reversed, randomized, or jittered scallops increased behavioral response thresholds, demonstrating that fish's electric signaling behavior was sensitive to the precise temporal structure of scallops. Next, using in vivo intracellular recordings and discriminant function analysis, we found that the responses of interval-selective midbrain neurons were also sensitive to the precise temporal structure of scallops. Subthreshold changes in membrane potential recorded from single neurons discriminated natural scallops from time-reversed, randomized, and jittered sequences. Pooling the responses of multiple neurons improved the discriminability of natural sequences from temporally manipulated sequences. Finally, we found that single-neuron responses were sensitive to interindividual variation in scallop sequences, raising the question of whether fish may analyze scallop structure to gain information about the sender. Collectively, these results demonstrate that a population of interval-selective neurons can encode behaviorally relevant temporal patterns with millisecond precision. SIGNIFICANCE STATEMENT The timing patterns of action potentials, or spikes, play important roles in representing information in the nervous system. However, how these temporal patterns are recognized by downstream neurons is not well understood. Here we use the electrosensory system of mormyrid weakly electric fish to investigate how a population of neurons with diverse temporal filtering properties encodes behaviorally relevant input timing patterns, and how this relates to behavioral sensitivity. We show that fish are behaviorally sensitive to millisecond variations in natural, temporally patterned communication signals, and that the responses of individual midbrain neurons are also sensitive to variation in these patterns. In fact, the output of single neurons contains enough information to discriminate stereotyped communication signals produced by different individuals.
Predictive factors of postoperative mortality after junctional and gastric adenocarcinoma resection.

IMPORTANCE Postoperative mortality after junctional and gastric adenocarcinoma resection remains a significant issue. OBJECTIVE To identify factors predictive of mortality within 30 days of junctional and gastric adenocarcinoma resection in a large national multicenter cohort. DESIGN A retrospective study collecting data from a multicenter database of patients who underwent resection for junctional and gastric adenocarcinoma from January 1, 1997, through January 31, 2010. A stepwise logistic regression model was built to identify, by multivariate analysis, variables independently predictive of 30-day postoperative mortality (POM). SETTING Nineteen university teaching hospitals in France. PARTICIPANTS Two thousand six hundred seventy patients with available data. MAIN OUTCOME MEASURES The primary end point was POM. Secondary end points included (1) late mortality (30-90 days after resection) and (2) postoperative morbidity. RESULTS One thousand eight hundred ninety-six patients (71.01%) had gastric adenocarcinoma and 774 (28.99%) had junctional tumors. Neoadjuvant treatment was given to 655 patients (24.53%), and 114 patients (4.27%) died within 30 days of surgery. Postoperative mortality was higher in patients who experienced grades III and IV toxic effects during neoadjuvant treatment compared with those who did not (8.7% vs 2.9%, respectively; P = .007). Multivariate analysis revealed metastatic disease at diagnosis (odds ratio, 9.13 [95% CI, 3.29-25.35]; P < .001) and poor tolerance of neoadjuvant treatment (3.33 [1.25-8.85]; P = .02) as being independently predictive of POM. Centers performing at least 10 resections per year were found to be protective against POM (odds ratio, 0.29 [95% CI, 0.12-0.72]; P = .008). CONCLUSIONS AND RELEVANCE This large national cohort study confirms that advanced disease heightens the risk of POM; centralization of junctional and gastric adenocarcinoma resection is warranted. The novel finding that grades III to IV toxic effects during neoadjuvant therapy increase POM has significant implications for decision making in this subgroup of patients. TRIAL REGISTRATION clinicaltrials.gov Identifier: NCT01249859.
Spinal cord and trigeminal projections to the pontine parabrachial region in the rat as demonstrated with Phaseolus vulgaris leucoagglutinin

In order to determine the regions within the parabrachial nucleus that receive synaptic input from nociceptive regions of the spinal cord and medulla in the rat, we analyzed the “Golgi‐like” labeling produced by anterograde transport of Phaseolus vulgaris leucoagglutinin (PHA‐L) from discrete iontophoretic injections confined to either the superficial dorsal horn of the lumbar spinal cord or to the superficial dorsal horn of the trigeminal nucleus at the level of the obex. Labeled fibers from both the spinal cord and the medulla ascended through the ventral lateral pons and coursed with the ventral spinocerebellar tract toward the parabrachial nuclei. Spinal cord injections led to labeling of fine caliber fibers and en passant and terminal enlargements in the rostral part of the contralateral lateral parabrachial nucleus (PBL), mostly in the central lateral and dorsal lateral subnuclei. Medullary injections revealed fiber and enlargement labeling primarily in the ipsilateral caudal PBL, mostly in the central lateral, external lateral, and medial subnuclei. Injections in both regions resulted in labeled terminations in the Kö;lliker‐Fuse nucleus. These results indicate that the nociceptive regions of the spinal cord and medulla terminate in regions of the parabrachial nucleus that have been associated with autonomic functions because of their interconnections with the hypothalamus, brainstem cardiovascular and respiratory control centers, and the amygdala. © 1994 Wiley‐Liss, Inc.
Patients' Perceptions of the Cosmetic Impact of Melanoma Resection

This study investigated the relationship between primary versus grafted closure for melanoma and the cosmetic impact on the patient of the resulting scar. A total of 176 patients with melanoma completed a multiple-choice questionnaire designed for this study. Two factors were significantly related to negative cosmetic impact: severity of scar indentation (but not scar length) and the extent to which patients were unprepared for the actual size of their scars. Patients whose scars were deeply indented as well as those whose scars were larger than anticipated displayed greatest distress about their appearance. Cosmetic impact was greater for women than for men. These observations suggest that primary closures, rather than closures requiring skin grafts, have important psychological benefits for patients and that physicians can assist postoperative adjustment by giving patients accurate information about the expected appearance of their scars.
Full polarimetric radar backscattering measurement of oil spilling indoor experiment

This paper reports on an experiment conducted at the wind-wave tank in UESTC microwave chamber to characterize the C- and X-band radar return from water surfaces covering oil films when observed at different incidences. The measurements of Normalized Radar Cross Section (NRCS) with bi-objective calibration technique were carried out for full polarization and various wind speeds. Comparisons are performed with clean sea water and diverse oil spills. From this data set we validate the Two-Scale Model (TSM) as a calculating method to simulate the backscatter coefficients of oil spill surface. The applicability of experimental results to SAR image extraction is discussed.
Employing Critical Reflexivity to Democratize Refugee Governance

This dissertation considers how refugees and internally displaced persons (IDPs) are conceived in international relations, and how they are understood in relation to the global refugee regime complex. This research explores how cognitive frames are impeding fair/democratic governance of IDPs/refugees and employs two case studies to investigate how the practice of critical reflexivity can lead to the creation of democratic spaces for refugees/IDPs to enact protection preferences. The first case analysis argues that Sarvodaya Shramadana’s Deshodaya initiative in Sri Lanka has enabled IDPs in that nation to embrace critical reflexivity to re-constitute/reimagine themselves as governing agents who can redefine state and international organization-based definitions of their protection. The second case examines asylum adjudications at the Department of Homeland Security and is an exploration of how that agency’s responsible officials can employ critical reflexivity to recognize seemingly hidden governance structures that condition their decision-making and limit refugee choices. Finally, this inquiry offers a new, organic model for conceptualizing both refugee/IDP governance and strategies for democratization of refugee/IDP governance institutions and systems. ***The views expressed here are those of the author and do not represent those of the United States Government. ***