Miracle-dz/UniFAR-FTUs huggingface dataset:
EclipseGavab: un entorno de desarrollo para la docencia online de la programación

En este trabajo se presenta EclipseGavab, un entorno de desarrollo integrado con caracteristicas colaborativas que permiten la implementacion del Aprendizaje Basado en Proyectos en titulaciones online de informatica. Dispone, entre otras caracteristicas, de mensajeria instantanea y edicion compartida de codigo, funcionalidades que permiten que el profesor supervise el trabajo de forma telematica y que los alumnos colaboren virtualmente. EclipseGavab soporta la programacion en varios lenguajes de programacion de distintos paradigmas y caracteristicas, entre ellos Pascal, C y Java, de forma que pueda utilizarse en diferentes asignaturas a lo largo de la titulacion.
Réponse biologique de cellules animales à des contraintes hydrodynamiques : simulation numérique, expérimentation et modélisation en bioréacteurs de laboratoire

La reponse globale de cellules animales a des contraintes hydrodynamiques lors de leur culture en suspension dans des reacteurs agites a ete etudiee grâce a une approche integrative couplant les outils du genie biochimique a ceux de la mecanique des fluides numerique. En premier lieu, la description de l’hydrodynamique moyenne et locale de deux systemes de culture agites de laboratoire, spinner et bioreacteur, a ete realisee. Puis, l'etude des cinetiques macroscopiques de cellules CHO cultivees en suspension, en milieu sans serum et sans proteine, a ete realisee avec differentes vitesses d’agitation, pour evaluer l'impact de l'agitation sur les vitesses de croissance et de mort cellulaires, ainsi que de consommation des substrats et de production des metabolites et de l'interferon-gamma recombinant. Des caracterisations supplementaires des cellules (apoptose, proteines intracellulaires) et de l'interferon ont egalement ete realisees. Les effets de l'intensification de l'agitation ont ete representes avec plusieurs correlations globales reliant : (i) en milieu contenant du pluronic, l'integrale des cellules viables au nombre de Reynolds, et la proportion de cellules lysees a la valeur moyenne de l'energie de dissipation, <[epsilon]? (ii) en milieu sans pluronic, les vitesses specifiques de croissance et de mort cellulaires a <[epsilon]. De plus, l'analyse par CFD de la distribution spatio-temporelle des contraintes indique que la lyse cellulaire, observee dans le reacteur aux conditions extremes d'agitation, serait plutot liee a des valeurs locales tres elevees de [epsilon], ainsi qu’a la frequence d'exposition des cellules dans ces zones energetiques. Un modele hydro-cinetique original, couplant l’hydrodynamique locale aux cinetiques cellulaires de croissance et de mort, et base sur l’intermittence de la turbulence permet la prediction de la lyse massive observee en reacteur sous certaines conditions. Pour confirmer le fait que les effets lies a l'intensification de l'agitation sont bien le resultat d'une augmentation des contraintes hydrodynamiques, et non d'une amelioration du transfert d'oxygene, ce dernier a ete mesure et modelise par couplage avec une simulation numerique de type Volume Of Fluid , concluant en une absence de limitation d'oxygene. Enfin, la conception, le dimensionnement et la caracterisation hydrodynamique d'un reacteur innovant de type Couette-Taylor, sont proposees pour la mise en œuvre de cultures perfusees dans un environnement hydrodynamique mieux controle
Fusobacterial brain abscess: a review of five cases and an analysis of possible pathogenesis.

OBJECT The cases of five patients with fusobacterial brain abscess are presented. The authors discuss their attempt to determine the pathogenesis. METHODS The clinical and microbiological features of five cases of fusobacterial brain abscess are reviewed. Isolates of 2031 Fusobacterium spp. and other anaerobes collected (1989-2002) at our institution were analyzed and compared for incidences and isolation sources. The findings were correlated with extensive literature on the subject. The five patients were men between 45 and 74 years of age. All experienced an insidious onset of the disease and probable hematogenous seeding of the organism(s). One patient had a monomicrobic Fusobacterium necrophorum abscess, whereas the others had polymicrobic F. nucleatum abscesses. Despite surgery and a regimen of antibiotic medications and dexamethasone, three patients experienced a paradoxical deterioration 3 days postoperatively that necessitated reevacuation of the lesion. The evacuants observed at that time contained numerous leukocytes but no microorganisms, suggesting intensified inflammation as the likely cause of deterioration. This explanation is supported by literature that fusobacteria strongly activate neutrophils. An analysis of the 2031 anaerobes from blood, wounds, and abscesses showed the considerable virulence of Fusobacterium spp. , which were able to enter and/or sustain themselves in the blood circulation. This pattern was similar to that of Clostridium spp. , but different from those of Peptostreptococcus spp. , Bacteroides spp. , and Prevotella spp. , which were less invasive but more abundant. CONCLUSIONS Some fusobacterial brain abscesses may be associated with a paradoxical postoperative deterioration, which is probably due to intensified inflammation following treatment. The blood-borne dissemination and invasive behavior of fusobacteria likely initiate such a brain abscess, and further seeding of other synergic bacteria leads to a polymicrobic abscess.
Whole gene deletion and splicing mutations expand the PINK1 genotypic spectrum

Autosomal recessive parkinsonism is a genetic condition closely resembling Parkinson disease, the only distinguishing features being an earlier age at onset and a slower disease progression. Three causative genes have been identified so far. While exon rearrangements are frequently encountered in the Parkin gene, most PINK1 mutations are represented by single nucleotide changes. We report a sporadic parkinsonian patient carrying a deletion of the entire PINK1 gene and a splice site mutation (g.15445_15467del23) which produces several aberrant mRNAs. This report expands the genotypic spectrum of PINK1 mutations, with relevant implications for molecular analysis of this gene. © 2006 Wiley‐Liss, Inc.
Self-adaptive Integer and Decimal Mutation Operators for Genetic Algorithms

Evolutionary algorithms are affected by more parameters than optimization methods typically. This is at the same time a source of their robustness as well as a source of frustration in designing them. Adaptation can be used not only for finding solutions to a given problem, but also for tuning genetic algorithms to the particular problem. Adaptation can be applied to problems as well as to evolutionary processes. In the first case adaptation modifies some components of genetic algorithms to provide an appropriate form of the algorithm, which meets the nature of the given problem. These components could be any of representation, crossover, mutation and selection. In the second case, adaptation suggests a way to tune the parameters of the changing configuration of genetic algorithms while solving the problem. In this paper two new self-adaptive mutation operators; integer and decimal mutation are proposed for implementing efficient mutation in the evolutionary process of genetic algorithm for function optimization. Experimentation with 27 test cases and 1350 runs proved the efficiency of these operators in solving optimization problems.
Indispensable role of peripheral progesterone level for the occurrence of prolactin surges in pseudopregnant rats.

Ovariectomy was performed in pseudopregnant (psp) rats on Day 1, 4 or 6 of psp (ovx-1, ovx-4 or ovx-6). Daily prolactin surges measured at 0500 h in ovx-1 rats occurred for 6 days similar to surges in chronically ovariectomized rats. However, prolactin surges at 0500 h in ovx-4 and ovx-6 rats stopped occurring immediately after ovariectomy. Progesterone supplementation by Silastic implants before the end of normal psp extended the occurrence of prolactin surges if the peripheral progesterone level was elevated to 80 ng/ml serum, but not when elevated to 20 ng/ml serum. Pseudopregnancy was extended up to 20 days by induction of ovulation on Day 6 of psp by hCG treatment. In these rats, prolactin surges lasted as long as peripheral progesterone stayed at an elevated level, namely until Day 18. These results suggest that neuroendocrine programming of cervical stimulation for the induction of prolactin surges is not retained for more than 4 days under a usual psp condition where corpora lutea start to secrete elevated amounts of progesterone from the afternoon of Day 3. After this period, the peripheral progesterone level must be solely responsible for the further maintenance of prolactin surges.
Projeto converge UFSM: rede de sensores-atuadores para controle de acesso em laboratórios usando voip e rede sem fio

E perceptivel, dentro da estrutura computacional que se tem hoje, o elevado grau de complexidade de gestao e operacao das diversas tecnologias existentes, as quais cada vez mais divergem entre si. Elas sao frequentemente utilizadas em areas como a de seguranca, onde se fazem indispensaveis os sistemas de computacao por eles oferecerem a possibilidade de automatizacao e reducao de custos. No projeto CONVERGE UFSM, o objetivo e prover uma convergencia de tecnologias como voz sobre IP e redes sem fio para o desenvolvimento de uma central telefonica digital e oferecer servicos de controle, acesso e alarme em um laboratorio. O projeto encontra-se em estado inicial de desenvolvimento e o artigo relata seus objetivos, a arquitetura da proposta, o ambiente de teste, bem como outros aspectos relevantes.
Gadolinium‐sensitive, voltage‐dependent calcium release channels in the endoplasmic reticulum of a higher plant mechanoreceptor organ.

The lipid bilayer technique was adapted to the functional reconstitution of ion channels from the endoplasmic reticulum of a higher plant. This was obtained at high purity from touch‐sensitive tendrils of Bryonia dioica. In this preparation, a calcium‐selective strongly rectifying channel is prevailing whose single‐channel properties have been characterized. The single‐channel conductance is 29 pS in 50 mM CaCl2. The Ca2+: K+ selectivity was determined to be approximately 6.6. The channel is voltage‐gated and, more importantly, the gating voltage is strongly shifted towards more negative voltages when a transmembrane Ca2+ gradient is applied. Thus, at physiological voltages across the endoplasmic reticulum membrane, the channel's open probability will be governed largely by the chemical potential gradient of Ca2+, generated by the Ca(2+)‐ATPase in that same membrane. The calcium release channel described here is effectively blocked by Gd3+ which also completely suppresses a tendril's reaction to touch, suggesting that this channel could be a key element of calcium signaling in higher plant mechanotransduction. Its molecular characteristics and inhibitor data show it to be the first known member of a hitherto unrecognized class of calcium channels.
Aryl Hydrocarbon Receptor Imported into the Nucleus following Ligand Binding Is Rapidly Degraded via the Cytosplasmic Proteasome following Nuclear Export*

The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that dimerizes with the AHR nuclear translocator protein to mediate gene regulation. However, the AHR protein is rapidly depleted in vitro and in vivo following exposure to ligands. The purpose of the studies in this report was to characterize the mechanism of AHR degradation and determine the consequence of blocking the degradation process. Western blot and immunological analysis of rat smooth muscle (A7), murine Hepa-1, and human HepG2 cells show that ligand-induced degradation of AHR is blocked when the proteasome is inhibited by MG-132. AHR degradation is also blocked in Hepa-1 and HepG2 cells when nuclear export is inhibited with leptomycin B. Mutation of a putative nuclear export signal present in the AHR results in the accumulation of AHR in the nucleus and reduced levels of degradation following ligand exposure. In addition, inhibition of AHR degradation results in an increase in the concentration of AHR·AHR nuclear translocator complexes associated with DNA and extends the duration that the complex resides in the nucleus. These findings show that nuclear export and degradation of the AHR protein are two additional steps in the AHR-mediated signal transduction pathway and suggest novel areas for regulatory control.
[Biomechanical modelling of the wrist joint].

BACKGROUND The hand represents one of the most complex joint mechanisms of the human body. The hand is also an important communication medium. The spectrum of today's hand injuries reaches from minor damage up to complex traumata with loss of several functional aspects. Enormous subsequent economic costs result. The therapeutic re-establishment of the equilibrium between maximum stress and the actual applied stress is the condition for a lifelong joint function. MATERIAL AND METHODS A literature review about biomechanical wrist models was realised. The previous models found in the literature were systematically analysed as well as verifying their suitability for clinical use regarding pathological changes, therapy approaches and modelling/simulation approaches, respectively, of wrist injuries. RESULTS The return of the wrist joint biomechanics to the normal condition is a key factor for a successful therapy. Furthermore, it is important for the re-establishment of an unimpaired joint function. Currently, there exist only simplified descriptions and models of the wrist joint, approximated by technical joints and furthermore, they are partially contradictory. Therefore, no uniform validated biomechanical wrist model exists as yet. CONCLUSION Regarding the arising complex clinical problems, however, a valid biomechanical wrist joint model would be necessary as assistance, in order to improve the success of systematised therapies on the basis of computer-aided model-based planning and intervention.
Soil mixing to decrease surface stratification of phosphorus in manured soils.

Continual applications of fertilizer and manure to permanent grassland or no-till soils can lead to an accumulation of P at the surface, which in turn increases the potential for P loss in overland flow. To investigate the feasibility of redistributing surface stratified P within the soil profile by plowing, Mehlich-3 P rich surface soils (128-961 mg kg(-) in 0-5 cm) were incubated with lower-P subsoil (16-119 mg kg(-1) in 5-20 cm) for 18 manured soils from Oklahoma and Pennsylvania that had received long-term manure applications (60-150 kg P ha(-1) yr(-1) as dairy, poultry, or swine manure for up to 20 yr). After incubating a mixture of 5 g surface soil (0- to 5-cm depth) and 15 g subsoil (5- to 20-cm depth) for 28 d, Mehlich-3 P decreased 66 to 90% as a function of the weighted mean Mehlich-3 P of surface and subsoil (i.e. 1:3 ratio) (r2 = 0.87). At Klingerstown, Northumberland County, south central Pennsylvania, a P-stratified Berks soil (Typic Dystrochrept) (495 mg kg(-1) Mehlich-3 P in 0- to 5-cm depth) was chisel plowed to about 25 cm and orchardgrass (Dactylis glomerata L.) planted. Once grass was established and erosion minimized (about 20 wk after plowing and planting), total P concentration in overland flow during a 30-min rainfall (6.5 cm h(-1)) was 1.79 mg L(-1) compared with 3.4 mg L(-1) before plowing, with dissolved P reduced from 2.9 to 0.3 mg L(-1). Plowing P-stratified soils has the potential to decrease P loss in overland flow, as long as plowing-induced erosion is minimized.
Risk Measurement and Disclosure in Islamic Finance and the Implications of Profit Sharing Investment Accounts

The paper discusses key issues in the measurement and control of risks in Islamic Financial Services Institutions, particularly the implications of profit sharing investment accounts (PSIA) for risk measurement, risk management, capital adequacy and supervision. Cross country data on a sample of banks reveal a considerable smoothing of returns paid to PSIA, despite wide divergences in risk. This suggests that the sharing of risks with PSIA is fairly limited in practice, although, in principle, well-designed risk (and return) sharing arrangements with PSIA can serve as a powerful risk mitigant in Islamic finance. Supervisory authorities can provide strong incentives for effective and transparent risk sharing and the associated product innovations, by linking the extent of capital relief on account of PSIA with appropriate supervisory review of the risks borne by the PSIA (equivalently the extent of displaced commercial risk assumed by the shareholders), and by requiring adequate disclosure of these risks. A value-at risk (VAR) methodology is proposed for measuring these risks.
Power efficient carry propagate adder

Here we describe the design details and performance of proposed Carry Propagate Adder based on GDI technique. GDI technique is power efficient technique for designing digital circuit that consumes less power as compare to most commonly used CMOS technique. GDI also has an advantage of minimum propagation delay, minimum area required and less complexity for designing any digital circuit. We designed Carry Propagate Adder using GDI technique and compared its performance with CMOS technique in terms of area, delay and power dissipation. Circuit designed using CADENCE EDA tool and simulated using SPECTRE VIRTUOSO tool at 0.18m technology. Comparative performance result shows that Carry Propagate Adder using GDI technique dissipated 55.6% less power as compare to Carry Propagate Adder using CMOS technique.
Synthesis of 5‐(1,2,3‐Triazol‐4‐yl)‐2′‐deoxyuridines by a Click Chemistry Approach: Stacking of Triazoles in the Major Groove Gives Increased Nucleic Acid Duplex Stability

A general protocol for converting alkyl and aryl halides into azides and for converting these in situ into 1,4‐disubstituted triazoles was applied with 5‐ethynyl‐2′‐deoxyuridine. This afforded three modified 2′‐deoxyuridine analogues with either unsubstituted or 1‐phenyl‐/1‐benzyl‐substituted triazoles in their 5‐positions. Modelling demonstrates coplanarity of the two heteroaromatic rings, and UV spectroscopy showed the uracil pKa values to be almost unchanged. The three nucleosides were introduced into nonamer oligonucleotides by phosphoramidite chemistry. The heteroaromatic triazoles became positioned in the major grooves of the short dsDNA and DNA–RNA duplexes. While single modifications led to decreased duplex stability, the stacking of four consecutive modifications led to enhanced duplex stability, especially for DNA–RNA duplexes. The duplex structures were studied by CD spectroscopy and molecular dynamics simulations, which supported the conjecture that the duplex stabilizing effect is due to efficient stacking of the heteroaromatic triazoles.
Right Hemisphere Activation during Indirect Semantic Priming: Evidence from Event-Related Potentials

Healthy subjects performed a lexical decision task in a semantic priming paradigm while event-related potentials (ERPs) were recorded from 64 channels. Semantic distance between prime and target was varied by including directly, indirectly, and nonrelated word pairs. At centro-parietal electrodes an N400 to nonrelated pairs was elicited bilaterally which was sensitive only to direct, but not to indirect semantic priming. These N400 priming effects were mirrored by the RT data. At inferior fronto-temporal sites directly related words showed ERP priming effects over both hemispheres. However, indirectly related words only elicited ERP priming effects over the right hemisphere. These results support the hypothesis that the right hemisphere semantic system is involved in processing of remote semantic information.
Contribution of linoleic acid to the formation of advanced glycation end products in model systems during heat treatment

Zhao X. , Yue L. , Liu X. , Liu L. (2017): Contribution of linoleic acid to the formation of advanced glycation end products in model systems during heat treatment. Czech J. Food Sci., 35: 367–375. Advanced glycation end products (AGEs) are glycosylated metabolic products generated in vivo and are associated with aging-related diseases. They are also formed during heat treatment in food processing. In this work, we investigated the contribution of linoleic acid (LA) to AGE formation using a protein/glucose model. An electronic tongue, denaturing polyacrylamide gel electrophoresis, electron spin resonance spectroscopy, circular dichroism, and ultraperformance liquid chromatography-tandem mass spectrometry were used to analyse reaction intermediates and reactive radical formation. The results show that LA is the key factor responsible for the change in flavour including the rapid triggering of glycation reactions. The amount of lipid-induced reactive radicals was significantly higher than in the non-fat system, radical generation in the non-fat system was gradually quenched after a robust radical-yielding reaction in the first 25 minutes. Subsequent unsaturated lipid oxidation, and AGE accumulation surpass Maillard reaction-only outcomes. Initial LA-induced changes in protein structure are followed by glycation and are enhanced by hydrophobic interactions and increased carbonyl levels resulting from lipid oxidation. These findings implicate lipids and lipid oxidation as the main factors responsible for AGE formation during the processing of fat-rich unsaturated fatty acid-containing foods.
Non‐parametric methods for doubly robust estimation of continuous treatment effects

Continuous treatments (e.g. doses) arise often in practice, but many available causal effect estimators are limited by either requiring parametric models for the effect curve, or by not allowing doubly robust covariate adjustment. We develop a novel kernel smoothing approach that requires only mild smoothness assumptions on the effect curve and still allows for misspecification of either the treatment density or outcome regression. We derive asymptotic properties and give a procedure for data‐driven bandwidth selection. The methods are illustrated via simulation and in a study of the effect of nurse staffing on hospital readmissions penalties.
Cue1p Is an Activator of Ubc7p E2 Activity in Vitro and in Vivo*

Ubc7p is a ubiquitin-conjugating enzyme (E2) that functions with endoplasmic reticulum (ER)-resident ubiquitin ligases (E3s) to promote endoplasmic reticulum-associated degradation (ERAD). Ubc7p only functions in ERAD if bound to the ER surface by Cue1p, a membrane-anchored ER protein. The role of Cue1p was thought to involve passive concentration of Ubc7p at the surface of the ER. However, our biochemical studies of Ubc7p suggested that Cue1p may, in addition, stimulate Ubc7p E2 activity. We have tested this idea and found it to be true both in vitro and in vivo. Ubc7p bound to the soluble domain of Cue1p showed strongly enhanced in vitro ubiquitination activity, both in the presence and absence of E3. Cue1p also enhanced Ubc7p function in vivo, and this activation was separable from the established ER-anchoring role of Cue1p. Finally, we tested in vivo activation of Ubc7p by Cue1p in an assay independent of the ER membrane and ERAD. A chimeric E2 linking Ubc7p to the Cdc34p/Ubc3p localization domain complemented the cdc34-2 TS phenotype, and co-expression of the soluble Cue1p domain enhanced complementation by this chimeric Ubc7p E2. These studies reveal a previously unobserved stimulation of Ubc7p E2 activity by Cue1p that is critical for full ERAD and that functions independently of the well known Cue1p anchoring function. Moreover, it suggests a previously unappreciated mode for regulation of E2s by Cue1p-like interacting partners.