Miracle-dz/UniFAR-FTUs huggingface dataset:
A Role of the C-terminal Region of Human Rad9 (hRad9) in Nuclear Transport of the hRad9 Checkpoint Complex*

Rad9, Rad1, and Hus1 are members of the Rad family of checkpoint proteins that are required for both DNA replication and DNA damage checkpoints and are thought to function as sensors in the DNA integrity checkpoint control. These proteins can interact with each other and form a stable proliferating cell nuclear antigen-related Rad9·Rad1·Hus1 heterotrimeric complex that might encircle DNA at or near the damaged sites. In this study, we demonstrate that the human Rad9 (hRad9) protein contains a predicted nuclear localization sequence (NLS) near its C terminus, which plays an essential role in the hRad9-mediated G2 checkpoint. Deletion experiments indicate that the NLS-containing region of hRad9 is critical for the nuclear transport of not only hRad9 but also human Rad1 (hRad1) and human Hus1 (hHus1), although this region is not required for hRad9·hRad1·hHus1 complex formation. In support of the role that hRad9 NLS plays in the nuclear targeting of the hRad9·hRad1·hHus1 complex, overexpression of a deletion mutant of hRad9 lacking the NLS-containing C-terminal region can bypass the G2 checkpoint and result in cell death after ionizing radiation or hydroxyurea treatment. Moreover, knockdown of hRad9 expression by small interfering RNA (siRNA) results in hRad1 accumulation in the cytoplasm and significantly abrogates the G2 checkpoint in the presence of damaged DNA or incomplete DNA replication. Thus, the C-terminal region of human Rad9 protein is important for G2 checkpoint control by operating the transport of the hRad9·hRad1·hHus1 checkpoint complex into the nucleus.
Analysis of Territorial Accessibility to the Nodes of Health Activity in the Municipality of Pitalito, Colombia

The current population growth links different social and economic development problems that must be addressed, within these public health, which should be of high priority in order to minimize the threats of health in the citizenry; therefore, administrative entities must get strong commitments in minimum periods of time. Taking into account the above, it is proposed to evaluate the level of accessibility offered by the road infrastructure network in the municipality of Pitalito, department of Huila, as well as the level of coverage by population and area for the years 2015 (base scenario) and 2031 (intervened scenario), through the use of geostatistical methods from digital tools.
Influence of aerobic exercise at high and moderate intensities on lipid peroxidation in untrained men.

AIM The purpose of this investigation was to evaluate the oxidative stress response to aerobic exercise at high and moderate intensities. METHODS Twenty healthy untrained men were randomly allocated into two groups: moderate intensity (MI) and high intensity (HI). Subjects of group MI and HI ran on the treadmill for 30-minutes at a running speed corresponding to 60% and 75% VO(2max) respectively. Blood lactate (LA) was measured before and immediately after the exercise. The subjective rate of perceived exertion (RPE) was obtained at the end of the trail. Venous blood samples were obtained before the exercise, immediately, 2 h, and 24 h after exercise. Blood samples were analyzed for malondialdehyde (MDA), as a biomarker of lipid peroxidation; uric acid (UA) as an intrinsic antioxidant; creatinin kinase (CK) activity, as a biomarker of muscle damage as well as total and differential leucocytes, as biomarkers of inflammation. The pattern of RPE was not significantly influenced by intensity of exercise. RESULTS There was no significant difference in LA, CK, MDA, UA, and total and differential leukocytes between two groups (P>0.05). MDA was increased 2 h after exercise only in group HI (P<0.05). Plasma UA concentrations and CK activities were significantly greater than pre-exercise in immediately and 2 h after exercise in both groups (p<0.05). Exercise resulted in significant leukocytosis immediately after exercise in both groups and 2 h after exercise only in group HI (P<0.05), returning to pre-exercise levels after 24 h just in group MI. Neutrophil counts were increased 2 h after exercise in both groups and 24 h after exercise only in group HI (P<0.05). Monocyte counts were increased 2 h after exercise only in group HI (P<0.05). CONCLUSION These data suggest that exercise with moderate intensity causes less lipid peroxidation and inflammation in comparison with high intensity exercise.
Relation of Respiratory System Elastance and Expiratory Time Constant: Are They From the Same Lung?

Abstract Modeling the respiratory system can aid clinical decision making during mechanical ventilation (MV) in the intensive care. However, most lung models only use airway pressure and flow data during inspiration phase of the breathing, and the data from expiration are often neglected. In this study, we hypothesized that the airway pressure and flow data during expiration have equal important information as the inspiratory data for respiratory mechanics modeling. In particular, expiratory time constant parameter, K, can provide unique information and relation with respiratory system elastance in MV patients. Two different respiratory models were investigated, with one model focusing on data from inspiratory cycle (Single compartment model) and the other focusing on expiratory cycle (Time constant model). The expiratory time constant model and single compartment model were evaluated based on 22 retrospective datasets of acute respiratory distress syndrome (ARDS) patients from two different cohorts. The expiration time constant model captured a moderate correlation with the lung elastance with R2= 0.568 in Cohort 1 and weak correlation with R2= 0.184 in Cohort 2, resulting in an overall of R2 = 0.435. Significant variations in lung resistance may lead to a poor correlation between K and lung elastance, Elung. Thus, the application of K as a surrogate to the respiratory elastance warrants further investigation.
B-cell activating factor promoter polymorphisms in egyptian patients with systemic lupus erythematosus.

BACKGROUND Systemic lupus erythematosus (SLE) is a heterogonous autoimmune disease involving most immune cells. Studies have revealed a number of cytokine pathways that play important roles in the disease process. Among these is B- cell activating factor (BAFF), which regulates B-cell maturation, survival, and function. OBJECTIVE To study the association between BAFF promoter polymorphism and systemic lupus erythematosus (SLE). METHODS Single nucleotide polymorphisms in the BAFF promoter region; -2841 (T>C), -2701 (T>A), -871 (C>T) were investigated by PCR-RFLP genotyping in fifty Egyptian SLE patients and thirty normal controls. RESULTS The frequency of mutant alleles of both -871C>T and -2701 T>A was higher among SLE patients than controls (p-value <0.001 and 0.000 respectively). There was a highly significant relationship between -871 C>T polymorphism and SLE (P<0.001), with the sensitivity and the specificity of the test being 100 %, and 70%, respectively. Patients expressing the -2701 T>A allele were seven times more prone to SLE than those with the T/T wild genotype (sensitivity of the test = 78%, specificity = 66.7%, odds ratio = 7.09, C.I at 95% = 2.29-22.64). CONCLUSION Polymorphisms in the regulatory region of the BAFF gene do contribute to the susceptibility to SLE in Egyptian patients, which indicates BAFF as a potential therapeutic target.
A 3 MHz two dimensional array based on piezocomposite for medical imaging

2-D array transducers present a major interest for ultrasound volumetric imaging as they allow data acquisition and visualization in real time. However their manufacture remains a technological challenge because of the very high number of elements with reduced lateral dimensions close to a half wavelength pitch. During this work, we developed and set up an industrial process to manufacture fully connected array through an innovative rear material, combining the backing function and the high density interconnect. This process does not affect the acoustical behavior of each elements and the connection of all elements is performed in a single operation. In addition, for the acoustical design, specific backing and matching layers structure have been implemented to reduce cross-talk and to increase the acceptance angle. A 2D fully populated array, based on this design, with a 3 MHz center frequency, 300 microns pitch, containing, 4096 elements (64/spl times/64) is presented. The evaluation of the performances over the array includes electroacoustical characterizations (pulse echo waveforms and spectrum), electrical measurements (electrical impedance and cross-talk) and acceptance angle data, obtained by hydrophone setup techniques. This design can be applied to many configurations, over a wide range of pitch and frequency, from fully populated to sparse array.
The effect of local water scarcity and drought on water conservation behaviors

ABSTRACT This study examines the influence of observed local water scarcity on individual water conservation behaviors. We examine three distinct sets dependent variables measuring water conservation behaviors that require differing levels of individual investment. These variables measure behaviors that require no financial investment (such as shorter showers), minimal financial investment (low-flow fixtures), and larger financial investment (new appliances). We use a multilevel statistical regression to determine if a relationship exists between local levels of drought and individual water conservation behavior. Our primary finding is that individuals who live in counties that experience drought have a higher tendency to make behavioral changes to conserve water and make small financial investments to conserve water. We find no relationship between local water scarcity and larger financial investments to conserve water. Overall, results suggest that individuals’ environmental behaviors may be closely connected to their local environmental context as long as significant financial investment is not required.
Batch scheduling model for distributed systems

Scheduling of jobs in distributed environment is proven to be NP-complete and has been addressed by various heuristics and metaheuristic approaches. It is always desired from a scheduling policy to dispense the load evenly on the available resources so as to have maximum utilization of resources while meeting the scheduling objective(s). Metaheuristics for job scheduling have gained immense popularity due to their effectiveness in providing near optimal solution in a reasonable time. This paper presents the working of batch scheduling model based on bacterial foraging. The model generates the schedule minimizing the node idle time and the makespan while exhibiting a balanced load distribution.
Cholesterol independent effect of LXR agonist TO‐901317 on gamma‐secretase

The balance of intracellular cholesterol has proven to be critical to the production of β‐amyloid (Aβ). Reducing cholesterol in vitro leads to decreased production of Aβ, whereas an increase in cellular cholesterol induces Aβ production. Liver X Receptor (LXR) agonists are known to increase cholesterol efflux from cells, but there are conflicting reports as to the effects of these agonists on Aβ production. We therefore examined the effects of efflux‐inducing agents on Aβ production in vitro. We used methyl‐β‐cyclodextrin and an LXR agonist (TO‐901317) to induce cholesterol efflux and studied the resulting Aβ production in a stable amyloid precursor protein (APP) ‐transfected cell line. When cholesterol efflux was induced with methyl‐β‐cyclodextrin there was a >60% decrease in Aβ40 and Aβ42 production. However, while activation of LXR using TO‐901317‐induced cholesterol efflux in the presence of a cholesterol acceptor, no changes in Aβ levels were recorded. When cells were incubated with TO‐901317 above the concentration required for maximal cholesterol efflux, there was a 150% increase in Aβ42 levels. The absence of a cholesterol acceptor from the culture media (preventing cholesterol efflux) did not blunt this increase in Aβ42, suggesting that the effects of TO‐901317 on Aβ42 are efflux independent. These results were confirmed in APP stably transfected human H4 cells, which revealed in addition to a 200% increase in Aβ42 levels, a concomitant 80% reduction in Aβ38. A cell‐free γ‐secretase assay confirmed that TO‐901317 can directly alter γ‐secretase activity. These data demonstrate that TO‐901317 can directly modulate the site of cleavage of APP by γ‐secretase in vitro.
Business Informatization Level

IT diffusion is central to the new economy and is reflected in a process of informatization of society and businesses. Although initially coined to represent the diffusion and adoption of information technology (IT) in all levels of society, the term informatization is also employed to represent the use of information technology resources in organizations. Weissbach (2003), for instance, defines informatization as being the process of gradual and increasing application of “planned and systematic use of IT penetrating the organization’s functions”. As pointed out by Lim (2001), the evaluation of an organization’s Informatization Level (IL) is an important managerial concern. The author also points out the difficulties associated with this evaluation, stating that “this is not a simple problem because informatization includes many intangible factors such as the quality of information and the organization’s culture”. The purpose of evaluating a company’s IL is to provide information for the organization to improve precisely its informatization level. It is also a means of benchmarking the efficacy and efficiency of IT investments in order to set up the baseline for improvement. This topic depicts a measurement method for the IL of companies and shows results of its application in 830 Brazilian industries (Zwicker, Vidal, & Souza, 2005). The development of this method was based on the principle that IT results in companies are not obtained merely through investments and the implementation of systems but rather through its proper use in business processes. The proposed method extends the informatization dimensions proposed by Lim (2001), using the process-based view of the IT business value creation model proposed by Soh and Markus (1995) and the concept of “information systems coverage” proposed by Ravarini, Tagliavini, Buonanno, and Sciuto (2002). BACKGROUND
Bike Station Correlation Network Construction Geographically-Constrained Station Clustering Urban Data ... Cluster-Level Bike Rental and Return Number Estimation Cluster-Level Over-Demand Probability Prediction Context Modeling Over-Demand ClustersDynamic Clusters Bike Stations Over-Demand Cluster

Bike sharing is booming globally as a green transportation mode, but the occurrence of over-demand stations that have no bikes or docks available greatly affects user experiences. Directly predicting individual over-demand stations to carry out preventive measures is difficult, since the bike usage pattern of a station is highly dynamic and context dependent. In addition, the fact that bike usage pattern is affected not only by common contextual factors (e.g., time and weather) but also by opportunistic contextual factors (e.g., social and traffic events) poses a great challenge. To address these issues, we propose a dynamic cluster-based framework for over-demand prediction. Depending on the context, we construct a weighted correlation network to model the relationship among bike stations, and dynamically group neighboring stations with similar bike usage patterns into clusters. We then adopt Monte Carlo simulation to predict the over-demand probability of each cluster. Evaluation results using real-world data from New York City and Washington, D.C. show that our framework accurately predicts over-demand clusters and outperforms the baseline methods significantly. ACM Classification
Depression Treatment Survey of Psychiatrists : Outcome and Side Effects

Objective: To explore psychiatrists’ opinions on the effectiveness and side-effects of current pharmacological antidepressant treatments in Australia. Method: A postal survey was sent to all consultant psychiatrists in Australia. Results: A total of 412 psychiatrists replied. The mean remission rate for depression reported by the sample was 54%. Following a partial but inadequate response to an optimal dose of either a Selective Serotonin Reuptake Inhibitors (SSRI) or Selective Serotonin/Noradrenaline Reuptake Inhibitors (SNRI), 42% (n=172) of respondents reported they would wash out the initial antidepressant and then start another antidepressant, 26% (n=106) would crosstaper antidepressants and 14% (n=59) would add a second antidepressant. Sexual problems, withdrawals, weight gain, perspiration and emotional blunting were reported as significant side-effects. Conclusion: The psychiatrists surveyed believed that depression in psychiatric practice had a guarded prognosis. Pharmacological agents commonly used for depression are perceived to have inadequate efficacy and a high side-effect burden. With regard to the effectiveness of treatment by family doctors, easier access to psychiatrists and psychologists, and better use of medication were suggested by psychiatrists as likely to improve outcomes for patients.
Evidence for a Neuroendocrinological Foundation of Human Affiliation

Although research on the neurobiological foundation of social affiliation has implicated the neuropeptide oxytocin in processes of maternal bonding in mammals, there is little evidence to support such links in humans. Plasma oxytocin and cortisol of 62 pregnant women were sampled during the first trimester, last trimester, and first postpartum month. Oxytocin was assayed using enzyme immunoassay, and free cortisol was calculated. After the infants were born, their interactions with their mothers were observed, and the mothers were interviewed regarding their infant-related thoughts and behaviors. Oxytocin was stable across time, and oxytocin levels at early pregnancy and the postpartum period were related to a clearly defined set of maternal bonding behaviors, including gaze, vocalizations, positive affect, and affectionate touch; to attachment-related thoughts; and to frequent checking of the infant. Across pregnancy and the postpartum period, oxytocin may play a role in the emergence of behaviors and mental representations typical of bonding in the human mother.
Pathological Changes in the Small Intestine of Neonatal Calves with Enteric Colibacillosis

Three neonatal calves, protected from colisepticaemia by intravenously administered immunoglobulin M, were infected orally with Escherichia coli type 0101K? (A). All calves developed severe enteric colibacillosis. When they were 4 days old stunting and fusion of villi were seen in the distal half of the small intestine and were associated with adhesion of the challenge organism to the mucosa. Two uninfected control calves kept under similar conditions, did not develop diarrhoea and had no lesions.
Make sure you understood your strategic partner in your buyer-supplier relationship

Buyer-supplier relationships have always been an important component in the long-term success of supply chains. This paper constructs a non-parametric Hoteling's T2 control chart to ensure that supply chain partners in dyadic relationships have the same level of understanding about the relationship. The proposed method is helpful to identify any unusual behavior in the relationship before it becomes a real problem.
Tuberculosis case finding in Rafsanjan : An epidemiologic study in Iran

1. MD. Rafsanjan University of Medical Sciences, Rafsanjan, Iran 2. MD. Assistant prof of Otolaryngology, Rafsanjan University of Medical Sciences, Rafsanjan, Iran 3. Medical student. Rafsanjan University of Medical Sciences, Rafsanjan, Iran 4. MD. Rafsanjan University of Medical Sciences, Rafsanjan, Iran 5. MD. Assistant Professor of psychiatry. Rafsanjan University of Medical Sciences, Rafsanjan, Iran 6. Bsc of Health policy. Rafsanjan University of Medical Sciences, Rafsanjan, Iran 7. MD. Rafsanjan University of Medical Sciences, Rafsanjan, Iran 8. Bsc of Microbiology. Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Atypical presentation of a Lumbar Intradural Breast-Cancer Metastasis: A Case report with Literature review*

I spinal tumors are usually primary tumors, and schwannoma, ependymoma and meningioma are most common ones at lumbar localization. Intradural metastasis accounts for very low percent of spinal metastases (7,15). Approximately 6% of spinal metastases are intradural. Most metastases are solitary lesions in configuration. Adenocarcinoma metastases are frequently originating from lung and breast. Intradural metastases reported in the literature are usually solitary renal cell carcinoma metastases (13-15,18). In current study, we present a case with intradural adenocarcinoma metastasis, which originated from lower thoracal region and involves entire lumbar spine, along with literature discussion.
The Stimulation Model for the Criterial Decision-Making at the Agricultural Enterprise

According to the general theory of systems, agricultural enterprises are from the category of closed systems in interaction with the external environment and they are close to the category of open systems. The decision maker (DM) needs to have information about the current state of the external environment with statistical analysis of the level achieved and forecasts for the future. Therefore, the task of improving the methods substantiating managerial decisions, using the criterion approach with simulation modeling of agricultural enterprises, is relevant. In the course of building the required model, three subtasks were solved: a conceptual model was built, which was transformed into a machine model, calculations were carried out, and the results were interpreted. Simulation modeling of the agricultural enterprise using a computer should study the performance of the agricultural enterprise before it is designed, at the stage of its setting up and in the course of its operation.
Injury mortality among children and teenagers in New Zealand compared with the United States of America.

OBJECTIVES: New Zealand (NZ) has an unenviable track record in childhood injury mortality. We sought to describe this burden and to compare it with the United States of America (USA), with a view to taking the first step in identifying potential areas in which NZ might benefit from injury control as practiced in the USA. METHODS: We identified all children and teenagers who had died of injury for the period 1984-93 from the NZ Health Information Service mortality data files. We compared their rates of injury with previously published rates for USA. RESULTS: The age specific rates follow a J shaped distribution, with high rates in the first year of life followed by a decline to the lowest rate, among 5-9 year olds, a marginally higher rate among 10-14 year olds, and a dramatic rise among those in the 15-19 age group. The specific causes of death vary considerably by age group. NZ's overall rate of child and adolescent injury is not substantially different from that of the USA, but marked differences are apparent when examining cause specific rates. CONCLUSIONS: In terms of the theoretical potential to reduce the total injury mortality rate, priority must be given to 15-19 year olds who account for 61% of all NZ injury deaths. Priorities for this age group are: motor vehicle traffic crashes (especially those involving occupants and motorcyclists), and suicide. Among the children, priorities are: pedestrian and occupant deaths, and drownings. Among infants, the priority is suffocation.