Miracle-dz/UniFAR-FTUs huggingface dataset:
Genomic and histological analysis of Helicobacter himalayensis sp. 1 himalayana to discovery the potential human pathogen

25 Background : Helicobacter himalayensis was isolated from Marmota himalayana in 26 the Qinghai-Tibet Plateau, China, and is a new non- H. pylori species, with unclear 27 taxonomy, phylogeny, and pathogenicity . 28 Methods and results : A comparative genomic analysis was performed between the H. 29 himalayensis type strain 80(YS1) and other the genomes of Helicobacter species 30 present in the National Center for Biotechnology Information (NCBI) database to 31 explore the molecular evolution and potential pathogenicity of H. himalayensis . We 32 also compared the lesions of gastrointestinal tissue samples from H. 33 himalayensis -positive Marmota himalayana with H. himalayensis -negative Marmota 34 himalayana using histology. H. himalayensis 80(YS1) T formed a clade with H. 35 cinaedi and H. hepaticus that was phylogenetically distant from H. pylori . The 36 genome of 80(YS1) T comprised 1,829,936 bp, with a 39.9% GC content, a predicted 37 genomic island, and 1,910 predicted proteins. Comparatively, H. himalayensis has 38 more genes for functions in “cell wall/membrane/envelope biogenesis” and 39 “nucleotide transport and metabolism” sub-branches than the other compared helicobacters, and its genome contained 42 virulence factors genes, including that 41 encoding cytolethal distending toxin (CDT). Histologically, the gastrointestinal mucosa of H. obvious lesions, implying that H. himalayensis is pathogenic to its host.
Continuous Membrane-Assisted Crystallization To Increase the Attainable Product Quality of Pharmaceuticals and Design Space for Operation

Continuous manufacturing is an important paradigm shift in pharmaceutical industries and has renewed the interest in continuous crystallization. The combination of crystallization and membranes is a promising hybrid technology for separation and purification of pharmaceuticals. The impact of membranes as an extension to conventional continuous crystallization processes on attainable product quality and design space is investigated systematically using model-based optimization. The proposed model is based on a full population balance such that all relevant crystallization phenomena can be included and is solved using a first-order discretization scheme with a hybrid grid. A case study involving continuous crystallization of paracetamol using a series of mixed suspension, mixed product removal (MSMPR) crystallizers is presented to illustrate the approach. The results show that the attainable size and design space can be enlarged significantly by extending conventional crystallization with membranes. In part.
Treatment of Early Seasonal Clogging of Sand Filters by Using Sterilized Rice Straw and Activated Carbon at EL- Fostat Water Plant

The present study was carried out in EL-Fostat water plant in order to find out the problem of early seasonal clogging of the sand filters during Autumn season and during the low level of the Nile River. This problem causes a lot of damages, e.g. losing of water during the back washing process and increasing of the used chemical doses used in the process of drinking water treatment (chlorine and aluminum sulphate). Also the study provides proper information on the phytoplankton communities, turbidity and phenol. The phenol experiment is very important to insure that the produced filtered water from the sterilized rice straw doesn't contain any phenolic compounds. Water samples were collected from stage1 (the raw water), stage 2 (after prechlorination), stage 3 (the clarifier) and stage 4 (the ordinary filters, sand only). The results revealed that the algae, especially diatoms (Synedra ulna) were the main cause of the early clogging of filters. In order to find a solution for this problem, several experiments were done by using two types of filters (activated carbon and sterilized rice straw beside the ordinary sand filters). These treatments proved the solving of this problem and helped to extend the filtration period to about 24 hours instead of 8 hours. So that this reduction of Journal of Environmental Sciences (JES) Institute of Environmental Studies and Research, Ain Shams University Mohammad, Bardees et al. Vol.(49); Iss. (8); No.(1); August 2020 ISSN 1110-0826 54 the washing time of the sand filter for one time, instead of three times leads to a provision of large quantities of water at ElFostat water plant and reduce the doses of the used chemicals.
Modeling and Spatialization of Biomass and Carbon Stock Using LiDAR Metrics in Tropical Dry Forest, Brazil

In recent years, with the growing environmental concern regarding climate change, there has been a search for efficient alternatives in indirect methods for the quantification of biomass and forest carbon stock. In this article, we seek to obtain pioneering results of biomass and carbon estimates from forest inventory data and LiDAR technology in a dry tropical forest in Brazil. We use forest inventory data in two areas together with data from the LiDAR flyby, generating estimates of local biomass and carbon levels obtained from local species. We approach three types of models for data analysis: Multiple linear regression with principal components (PCA), conventional multiple linear regression and stepwise multiple linear regression. The best fit total above ground biomass (TAGB) and total above ground carbon (TAGC) model was the stepwise multiple linear regression, concluding, then, that LiDAR data can be used to estimate biomass and total carbon in dry tropical forest, proven by an adjustment considered in the models employed, with a significant correlation between the LiDAR metrics. Our finding provides important information about the spatial distribution of TAGB and TAGC in the study area, which can be used to manage the reserve for optimal carbon sequestration.
Tuning of conditions for light-driven characterization of poly(phenylacetylene)s

: Poly(phenylacetylene)s are a family of dynamic helical polymers where the elongation and the helical sense of the double helix can be easily tuned in a controlled way using the proper external stimuli. Since there is a direct relationship between the secondary structure of the polymer and its potential applications, the structural elucidation of these polymers is nowadays a challenge in polymer chemistry. These polymers are characterised using the combination of different techniques such as CD, UV-Vis, Raman, DSC… However, the interpretation of the data is time consuming and in occasions difficult, being necessary to resort to AFM 4 . Due these obstacles, we focus on the light-driven cyclization of PPAs as a fast and easy-going alternative approach to decipher the configuration of the polyene backbone. Several parameters must be considered to achieve acceptable values of reproducibility and repeatability on this technique. On this work, we evaluate the influence of the relation between volume/concentration of sample and photoabsorption on PPAs when monitoring results by UV-Vis, resulting in and easy and controlled way to tune the light-driven characterization of PPAs.
Transferrin-targeted poly(butylene adipate)/terephthalate nanoparticles for targeted delivery of 5-fluorouracil in HT29 colorectal cancer cell line

The purpose of this study was to design 5-fluorouracil-loaded poly(butylene adipate)/terephthalate (Ecoflex®) nanoparticles for targeting colorectal cancer. The nanoparticles were prepared by emulsification–solvent evaporation method and optimized by a full factorial design. The effects of polymer and surfactant concentration, surfactant type, and stirrer rate were studied on the particle size, zeta potential, loading efficiency, and release efficiency of nanoparticles. For production of targeted nanoparticles, chitosan was conjugated to transferrin which was then coated on the surface of Ecoflex nanoparticles via electrostatic interactions. The conjugation of transferrin/chitosan was verified by Fourier transform infrared spectroscopy, ultraviolet spectroscopy, and SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis) methods and quantified by ultraviolet spectroscopy assay. The cytotoxicity of 5-fluorouracil loaded in targeted and non-targeted nanoparticles was studied on human colon adenocarcinoma cell line (HT29), Michigan Cancer Foundation-7 (MCF-7), and human umbilical vein endothelial cells using MTT (thiazolyl blue tetrazolium bromide) assay. The best results were obtained from nanoparticles prepared by 0.2% of the polymer, 2% of Tween 20, and stirrer speed of 17,500 r/min. The successful conjugation of transferrin/chitosan was confirmed by Fourier transform infrared spectrum and SDS-PAGE results and was about 80%. The targeted nanoparticles showed significantly more cytotoxic effects on HT29 cells compared to free 5-fluorouracil and non-targeted nanoparticles. Blocking transferrin receptors resulted in a significantly higher cell survival for targeted nanoparticles which confirmed receptor-mediated cellular uptake of targeted nanoparticles.
Machine-learning prediction of the d-band center for metals and bimetals

The d-band center for metals has been widely used in order to understand activity trends in metal-surface-catalyzed reactions in terms of the linear Bronsted–Evans–Polanyi relation and Hammer–Norskov d-band model. In this paper, the d-band centers for eleven metals (Fe, Co, Ni, Cu, Ru, Rh, Pd, Ag, Ir, Pt, Au) and their pairwise bimetals for two different structures (1% metal doped- or overlayer-covered metal surfaces) are statistically predicted using machine learning methods from readily available values as descriptors for the target metals (such as the density and the enthalpy of fusion of each metal). The predictive accuracy of four regression methods with different numbers of descriptors and different test-set/training-set ratios are quantitatively evaluated using statistical cross validations. It is shown that the d-band centers are reasonably well predicted by the gradient boosting regression (GBR) method with only six descriptors, even when we predict 75% of the data from only 25% given for training (average root mean square error (RMSE) < 0.5 eV). This demonstrates a potential use of machine learning methods for predicting the activity trends of metal surfaces with a negligible CPU time compared to first-principles methods.
Vortex Induced Vibrations Analysis of a Cantilevered Blunt Plate by Proper Orthogonal Decomposition of TR-PIV and Structural Modal Analysis

The present work focuses on the experimental characterization of the vortex shedding and on the induced vibrations of a cantilevered blunt rectangular aluminum plate of chord to thickness ratio 16, immersed in a uniform water flow in the hydrodynamic tunnel of the French Naval Academy Research Institute. Experiences have been conducted for Reynolds numbers Re (based on chord length) ranging from 2.5 × 105 to 10.5 × 105 at zero degrees incidence. Special attention has been paid to the interaction of the structural response and the flow dynamics at the twisting resonance. For this purpose, wake structures have been analyzed by Time Resolved Particle Image Velocimetry (TR-PIV) and the structural response of the plate has been examined by laser vibrometry. The von Karman vortex street has been characterized by statistical analysis and Proper Orthogonal Decomposition of PIV velocity fields and the structure is analyzed through modal analysis. The near-wake’s structure has been examined for three different Reynolds numbers: (i) at Re = 3.0 × 105, corresponding to vortex induced structural response at constant Strouhal number; (ii) at Re = 4.5 × 105, corresponding to mechanical resonance but dissociated vortex shedding and (iii) at Re = 5.4 × 105, corresponding to lock-in of the vortex shedding at the mechanical resonance. At Re = 4.5 × 105, at mechanical resonance, it reveals the occurrence of an energy transfer between the shear layer and the bubble wake vortex which cancels synchronization of the structural vibration with the von-Karman vortex street.
Prediction of the crowd behavior in campus based on time series model

Recognizing and understanding the behavior of the campus crowd is of great significance for improving the comprehensive management capabilities of the campus. The increasingly abundant of mobile terminal WIFI signals provide a unique data source for studying the behavior of the campus crowd. A Wifi-based personnel activity monitoring system is developed in this paper. Through monitoring and analyzing the MAC address (Media Access Control Address), RSSI (Received Signal Strength Indication) and Wireless AP (Access Point) in and around the Science Museum, Huaizhou building of Yunnan University, the association between WIFI signal and the crowd behavior is studied. Time series analysis is used to make predictions, and some valuable information about the gathering and activity status of campus crowd behavior is obtained. It provides an experimental platform for studying the dynamic behavior of people in campus or other crowd areas.
THE CORRELATION BETWEEN TROPONIN I LEVEL WITH CORRECTED THROMBOLYSIS IN MYOCARDIAL INFARCTION FRAME COUNT IN PATIENTS WHOSE UNDERWENT PRIMARY PERCUTANEOUS CORONARY INTERVENTION: A SINGLE CENTER STUDY

Objective: There were studies reported about the correlation between troponin I with scale of myocardial infarction (MI) area. However, the effect of revascularization into the coronary flow has not been reported. We aim to determine the correlation between high sensitivity troponin I and the successfulness of revascularization primary percutaneous coronary intervention (PPCI) in ST-elevation MI (STEMI) patients. The successfulness of revascularization was assessed by calculating Corrected thrombolysis in MI frame count (CTFC). Methods: This is a retrospective analysis including all consecutive patients undergoing PPCI over 1-year period from December 2018 to 2019 at the Integrated Heart Service Installation Dr. M. Djamil Padang Hospital, West Sumatra. Exclusion criteria included patients with incomplete medical record data, incomplete coronary angiography data, prior MI, and prior PCI and coronary artery bypass graft. Patients were put into two groups with normal (CTFC <30) and high (CTFC ≥30) to define the baseline characteristics. Results: A retrospective analysis was performed of patients undergoing primary PCI. Ninety-six patients underwent PPCI between December 2018 and 2019 were enrolled. For the baseline characteristic, patient was divided into two groups with normal CTFC (<30) and high (CTFC ≥30). There was negative weak correlation between troponin I level and CTFC with Spearman’s correlation value was 0.371 and correlation coefficient −0.092. Conclusion: Troponin I level at first presentation before revascularization did not have a significant correlation with coronary flow result after revascularization in STEMI patients.
Safe anesthesia, analgesic and sedation in the surgery of head and neck tumors

Highly traumatic surgical interventions for head and neck tumors were performed in 62 adult patients under conditions of multimodal opioid-free general anesthesia, which included dexmedetomidine, lidocaine, nefopam, and sevoflurane. In 18 cases, due to trismus II – IV degree, fibro optic nasotracheal intubation was used, in 10 patients with laryngeal stenosis, the operation was started with tracheotomy under local anesthesia. In all 28 cases, local anesthesia was supplemented by sedation with intravenous (IV) administration of dexmedetomidine and lidocaine, which was enhanced by the addition of subdrug doses (10–20 mg) of ketamine. Consciousness and spontaneous breathing were preserved in all observations. Before intubation, propofol and rocuronium were added, and at the stage of the main operation, against the background of intravenous infusion of dexmedetomidine and lidocaine, inhalation of sevoflurane vapors (1–1.5 MAC). In 62 cases, the course of the operation and anesthesia was smooth, awakening and restoration of spontaneous breathing occurred after the end of the operation. Postoperative pain relief for the first two days was in the form of a continuous iv infusion of a 3-component mixture of 1% lidocaine and two non-opioid analgesics (nefopam and tenoxicam) using a disposable elastomeric pump. In the next 3-4 days, they switched to intramuscular administration of nefopam and tenoxicam. The quality of pain relief was high, without clinically significant complications. Only in three cases, at the beginning of mastering the technique, it was necessary to resort to an additional single injection of promedol or tramadol.
Screening of some rice genotypes for aerobic condition in Northeast India

In an experiment conducted during 2015-2016, about 150 rice genotypes from different ecotypes were collected and screened for drought response. Out of 150 genotypes, some genotypes showed tolerance under drought condition. The genotypes were grouped into three numbers viz. highly tolerant (HT, survive 25-37 DAS), medium tolerant (MT, 20-24 DAS), susceptible (ST, 10-19 DAS) based on germination, survival and growth from days of sowing. After screening, five genotypes viz. Banglami, Inglongkiri, Boga ahu, Ronga ahu, and Bengungutia ahu were selected and evaluated to understand the mechanism of tolerance under aerobic and normal condition by comparing with aerobic rice variety CR Dhan. Some genotypes viz. Ronga ahu, Inglongkiri and Banglami recorded higher seedling vigour index (SVI), therefore seed of those genotypes easily survived for even 30-35 days under aerobic condition. Thus identification of long-coleoptile trait in rice may improve seedling establishment and yield stability of the rice under aerobic condition in farmers’ field. Such genotypes may be used for future breeding material for moisture stress condition.
The Physical and Social Environments as Determinants of Health: Implications for Substance and Behavioral Addictions

Whether unintentional or by design, built, social, and perceived environments influence the human experience. Behavior is not solely the product of a rational motivated actor, operating independently from his or her environment; rather, it is also a function of edifices, neighborhoods, and public spaces, as well as the inhabitants, community norms, and the social capital they generate. Likewise, addictive behaviors have as much to do with the environmental contexts surrounding individuals as with their unique biological factors, specific brain mechanisms, and psychogenic causes. Any attempt to address addiction at either individual or population levels would benefit from careful consideration of the social and contextual influences on cognitions, opportunities, motivations, and behaviors. Interventions informed by this understanding are more likely to be efficacious than those solely targeted toward individual biology, motivations, or attitudes. In this chapter, we discuss the relationship between physical and social environments (PSE), health, and the behavior of humans. We then focus on the influential role of the PSE on the consumption of alcohol, tobacco, and other substances; food, eating behaviors, and addictions contributing to the current obesity epidemic; and a selection of other behavioral addictions. The chapter closes by discussing methodological considerations and implications for professional practice.
The Speech Acts Recognition of ESP Students with and without Body Language

This paper mainly deals with recognition of speech acts of body language by suggesting various types of body language in order to achieve the study objectives which aim at identifying the difference between speech act recognition of the ESP students with and without body language, once by listening to the speaker then by listening and watching the speaker body language of the speaker. This study is limited to 1st year students at Computer Science department \ College of Science for Women at University of Baghdad. Two listening tests are designed which consist of various types of speech acts in the audio that suit ESP university students’ level of English language. In the first test, the students listened to the audio voice of the speaker talked about English proverbs and the students are asked to tick the speech acts items each time they appear, while in the second test, the same students listen and watch videos of body language of the same speaker talking about the same subject and they are also asked to tick the items of speech acts that they are ticked in the first test each time they appear. Results show that there is a significant difference between the speech acts tests by listening to the audio recording of the presenter on one hand, and watching the video recording on the other hand, and in favour of watching the video that used body language. The results validated listening and watching the video since the computed value of the t- test (1.98) is higher than the tabulated t- value (1.69) at (29) degree of freedom and (0.05) level of significance.
Construction of artificial miRNA expression cassettes targeting hTeRT and their inhibitory effects on telomerase activity in HepG2 cells

Objective: To construct the artificial miRNA expression cassettes targeting hTERT gene and validate their inhibitory effect on telomerase activity in HepG2 cells. Methods: Three artificial miRNA expression cassettes targeting different sites of hTERT gene were designed and constructed by overlap extension PCR method. After identification, the cassettes were transfected alone or cotransfected into HepG2 cells, and then the telomerase activities in HepG2 cells were detected by TRAP-silver staining and TRAP-duplex scorpion probe fluorescence quantitative PCR. Results: The three artificial miRNA expression cassettes targeting hTERT gene were all successfully constructed, and after they were transfected alone or co-transfected into the HepG2 cells, the telomerase activities in HepG2 cells were inhibited to different degrees, and the inhibitory effect of co-transfection with two miRNA expression ·基础研究· 基金项目:中南大学本科生自由探索计划项目资助(2282014bks193)。 收稿日期:2014-10-30; 修订日期:2014-11-25。 作者简介:徐慧,中南大学湘雅医学院硕士研究生,主要从事生物化学与分子生物学诊断方面的研究。 通信作者:彭剑雄, Email: jxpeng@csu.edu. cn doi:10.7659/j.issn. 1005-6947.2014. 12.009 http://www.zpwz. net/CN/abstract/abstract4131.shtml 中国普通外科杂志 第 23 卷 1648 ©版权归中国普通外科杂志所有 http://www.zpwz. net 端 粒 酶 与 恶 性 肿 瘤 的 发 生、 发 展 关 系 密 切, 通过端粒酶途径,端粒 DNA 能得以保持,使细胞 永 生 。 人 端 粒 酶 逆 转 录 酶(human telomerase reverse transcriptase,hTERT) 是 催 化 这 一 反 应 的限速酶,抑制 hTERT 基因的表达可以降低端粒 酶活性,从而抑制肿瘤细胞的分裂和增殖 , 已成为 肿瘤基因治疗的理想靶点 。 针对 hTERT 基因, 本实验室首先利用体外合成的方法制备 shRNA, 接 着 利 用 融 合 PCR 技 术 构 建 了 以 双 H1 启 动 子 和 U6/H1 双启动子驱动的 siRNA 表达框架 ,结果 均显示端粒酶活性明显降低。 人 工 miRNA 表 达 框 架 是 一 种 由 PCR 得 到 的 miRNA 表达模版,该模板以内源 miRNA 前体作为 骨 干, 将 pre-miRNA 置 换 为 设 计 好 的 序 列, 利 用 miRNA 的内源性作用机制来抑制特定的靶基因 。 本 实 验 应 用 构 建 靶 向 hTERT 基 因 的 人 工 miRNA 表达框架转染 HepG2 细胞,研究其对端粒酶活性 的抑制作用,为肿瘤抑制探索新的途径。