Miracle-dz/UniFAR-FTUs huggingface dataset:
Wild-Type Opaque2 and Defective opaque2 Polypeptides Form Complexes in Maize Endosperm Cells and Bind the Opaque2-Zein Target Site1[OA]

The Opaque2 (O2) basic leucine (Leu)-zipper transcriptional activator controls the expression of several genes in maize (Zea mays). We investigated the phosphorylation extent of wild-type O2 and mutant-defective or mutant-truncated o2 polypeptides in endosperm cells, their subcellular localization, participation in complex formation, and involvement in functional activity. Besides wild type, four mutant alleles (o2T, o2-52, o2It, and o2-676) producing o2 polypeptides and a null transcript allele (o2R) were considered. Observing the effects of these mutations, multiphosphorylation events in O2 or o2 proteins were confirmed and further investigated, and the involvement of both the nuclear localization signal (NLS)-B and Leu-zipper domains in proper targeting to the nucleus was ascertained. The absence of these domains in the o2T and o2It-S mutant-truncated forms holds them within the cytoplasm, where they are partially phosphorylated, whereas the presence of NLS-B and a partial Leu-zipper domain in o2-52 distributes this mutant-truncated form in both cytoplasm and nucleus. Although mutated in the NLS-B domain, the o2It-L and o2-676 mutant-defective forms are, respectively, partially or completely distributed into the nucleus. Only wild-type O2 and mutant-defective o2 polypeptides bearing the Leu-zipper are able to form complexes whose components were proven to bind the O2-zein target site by in vitro analyses. The transcription of a subset of H-zein genes as well as H-zein polypeptide accumulation in several o2-mutant-defective genotypes indicate the in vivo involvement of o2-mutant-defective proteins in O2-zein target site recognition. The gathered information broadens our knowledge on O2 functional activity and our view on possible quality protein maize trait manipulation or plant transformation via the utilization of cisgenic elements.
Subarray incoherency compensation for DOA estimation of 8-element antenna array

MUSIC algorithm is one of the most widely known and used signal source estimation techniques. A presented paper deal with an experiment of direction of arrival (DOA) estimation of signal sources using two subarrays without coherency or with rough synchronization. The performance of DOA estimation improves with increasing the number of elements of antenna array. In this case, a practical measurement is performed using 8-element antenna array (each antenna subarray includes 4-element), received signals between two antenna subarrays are roughly synchronized. For estimation DOA signals we proposed an algorithm to estimate DOA signal using MUSIC algorithm with higher accuracy. Results show that the algorithm could estimate DOA signals correctly in the case of two incoming coherent signals.
KLUVER-BUCY SYNDROME IN A BOY WITH NON-HODGKIN LYMPHOMA

Kluver-Bucy syndrome is a rare neurobehavioral condition characterized by visual agnosia, excessive oral tendency, hypermetamorphosis, placidity, altered sexual behaviors, and changes in dietary habits. The authors report a case of Kluver-Bucy syndrome in a 10-year-old boy with non-Hodgkin lymphoma after intratechal methotrexate administration. He was treated by risperidone without any sequels.
Analysis of the DMBT1 gene in carcinomas of the respiratory tract

Loss of chromosome 10q is a critical step during the progression and metastasis formation of lung cancer. We recently defined 3 distinct regions of allelic imbalances and considered the DMBT1 gene at 10q25‐q26 an interesting candidate for the most telomeric region. Therefore, we investigated DMBT1 in 25 cancer cell lines and 39 primary tumors of the respiratory tract. The analysis by RT‐PCR and Northern blot hybridization revealed that the gene is expressed in all tumors and cell lines and diminished in the SCLC line H187, indicating that RT‐PCR is critical when used as the single method for the evaluation of gene expression. No mutations were found by SSCP analysis of the cDNA and the partially known genomic sequence. Similarly, Southern blot hybridization was unable to detect homozygous deletions. Allelotyping of the markers D10S587, D10S1708 and D10S1723 located near or within the DMBT1 gene did not reach the peak incidence of the 3 minimally deleted regions that we recently defined. In summary, our data do not confirm previous findings reporting frequent loss of DMBT1 expression in lung cancer. However, they strengthen the notion that the responsible gene on chromosome 10q25‐q26 mediating tumor progression and metastasis formation in respiratory tract cancer remains enigmatic. Int. J. Cancer 88:71–76, 2000. © 2000 Wiley‐Liss, Inc.
Cost-effectiveness of influenza vaccination for elderly people living in the community.

Principal applicant and corresponding author: Prof Sarah M McGhee Department of Community Medicine, The University of Hong Kong, 21 Sassoon Road, Pokfulam, Hong Kong SAR, China Tel: (852) 2819 9193 Fax: (852) 2855 9528 E-mail: smmcghee@hkucc.hku. hk CM Schooling 舒 菱 LC Wong 黃麗君 J Chau 周宗欣 A Cheung 張惠棱 A Ho 何麗儀 SM McGhee 麥潔儀 Cost-effectiveness of influenza vaccination for elderly people living in the community
Joint Study of Extracranial Arterial Occlusion as a Cause of Stroke: I. Organization of Study and Survey of Patient Population

Between July 1, 1961, and June 30, 1965, 4,748 patients with signs and symptoms of ischemic cerebrovascular disease were admitted to various institutions for prospective investigation. The mean age for the group was 60 years. Two thirds were below 65 years of age. A significantly larger proportion of nonwhites were in the age group below 55. Eighty-four percent were white, and men outnumbered women 2:1. Interinstitutional differences in clinical status of patients were noted, particularly between vascular surgical and neurologic services. Complete arteriographic studies showing intracranial and extracranial circulation were accomplished in 80%. Twenty-five percent of the patients had no lesions demonstrated or had surgically inaccessible lesions only. The remainder had surgically accessible lesions, both with or without inaccessible lesions. A higher proportion of patients with surgically accessible lesions was noted among whites.
Semantische Adaption von Komponenten

Within the field of information technology, the adaptation of software as a result of changes in the environment has always been an important research topic. The costs and efforts to change an existing software largely stem from the programming language, design patterns or the the underlying software architecture. Furthermore, it has always been interesting to develop new techniques that can enhance applications to adapt autonomously to spontaneous changes within their environment. This technique, also called context-sensitive adaption can change the structure, the functionality or the behaviour of the software at runtime. This is particularly important in the area of ubiquitous computing, in which increasingly smaller devices like smartphones help people to perform their daily activites. Context-sensitive systems can hereby adapt applications interactively according to the concrete needs of users. However, an implementation of such a functionality also bears a number of technical hurdles. Since most of the changes occur spontaneously and cannot be anticipated, it is difficult to take them into account during the software design. In addition, the adaptation should take place as transparently as possible. Developers of applications also do not have a detailed knowledge about the internals of the applications that should be adapted. In this dissertation, the Aspect-Oriented Component Infrastructure (AOCI) framework will be introduced, which helps to provide context-sensitive adaptation in ubiquitous environments. Within AOCI, the environment, applications and adaptations are described precisely by means of expressions. This information is stored within a model with well-defined semantics and is used at runtime as a kind of extended contract. This can be used to determine which situations require to perform an adaptation and where it can be applied to. The model has been implemented by means of an ontology. In addition, AOCI uses different adaptation techniques to perform the adaptation at runtime. The central aspects of this thesis are therefore the ontological model and the implementation and integration of adaptation techniques that allow to provide context-sensitive adaption as sketched above.
Pre-eclampsia, placental abruption and increased risk of atopic sensitization in male adolescent offspring.

PROBLEM The aim of this study was to investigate maternal pre-eclampsia (PE), placental abruption (PA) and atopy among offspring. METHOD OF STUDY Obstetric data was recorded from 378 women who were included into the Caesarean delivery birth cohort during 1990-1992. When their children were 15-17 years old skin prick tests (SPTs) and inhalant allergen-specific (IAS) immunoglobulin E (IgE) were determined among offspring. RESULTS Adolescents whose mothers had either PE or PA at the time of delivery had more atopy and especially more severe atopy as measured by the incidence of SPTs positive for > or = 5 allergens (RR: 4.28, 95% CI: 1.54-11.92; P < 0.005) and elevated IAS IgE levels (> or = 1.0 kU/L) (RR: 4.27, 95% CI: 1.08-16.99; P < 0.039). This increased risk was particularly observed in male adolescents. CONCLUSION Maternal PE and PA were associated with an increased risk of severe atopy, especially in male adolescent offspring.
Two overlapping sequence motifs within the polyomavirus enhancer are independently the targets of stimulation by both the tumor promoter 12-O-tetradecanoylphorbol-13-acetate and the Ha-ras oncogene

A tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), strongly stimulates the activity of polyomavirus enhancer in a human erythroleukemia cell line, K562. The target of stimulation was the previously defined A element (from nucleotides 5107 to 5130) of the enhancer. We found that within the A element, two partly overlapping sequence motifs (one from nucleotides 5107 to 5117, the other from nucleotides 5113 to 5121) were independently the targets of TPA stimulation. The former is homologous to the enhancer core sequence of the adenovirus type 5 E1A gene, and the latter shares the consensus AP-1-binding site. In addition, transiently expressed Ha-ras oncogene also stimulated these two subelements in K562 cells, as we reported for NIH 3T3 cells previously.
A radial basis function neural network based efficiency optimization controller for induction motor with vector control

In this paper, a efficiency optimization controller is studied for a vector controlled induction motor. The optimum flux-producing current is obtained utilizing radial basis function neural network (RBFNN). Comparing with the conventional neural network, the radial basis function neural network possesses characteristic of simple structure, fast convergence and strong generalization, it is suitable for real-time control. Considering the change of iron core loss resistance due to flux and frequency, a precise dynamic model of induction motor is built, and RBFNN is trained based on this model. When induction motor is in steady state, the trained RBFNN is used to optimize flux-producing current; while when induction motor is in the transient state, the flux-producing current recovers the rated value for ensuring the fast response. The simulation is done with Matlab/Simulink and the proposed method of control is realized adopting TMS320LF2407A. Simulation and experiment results show that the efficiency optimization is significant and speed response is rapid.
High‐dose anabolic androgenic steroids modulate concentrations of nerve growth factor and expression of its low affinity receptor (p75‐NGFr) in male rat brain

The effects of treatment with a high dose of nandrolone or testosterone on nerve growth factor (NGF) levels and NGF low‐affinity receptor (p75‐NGFr) distribution in the brain were analyzed. Nandrolone, subcutaneously injected in rats for several weeks, caused an increase of NGF levels in the hippocampus and septum and a decrease in the hypothalamus. The number of p75‐NGFr‐immunoreactive neurons and the p75‐NGFr expression levels were reduced in the septum and vertical and horizontal Broca's bands. Testosterone injections caused an increase of NGF levels in the hippocampus, septum, and occipital cortex and induced an upregulation of p75‐NGFr in the forebrain NGF target regions. This testosterone effect suggests that nandrolone and testosterone affect brain NGF target cells by a different mechanism(s). Nandrolone may interfere with NGF transport and/or utilization by forebrain neurons, causing an altered p75‐NGFr expression and NGF accumulation as a consequence. Since NGF is known to maintain forebrain neurons and to regulate neurobehavioral functions, including memory, learning, and defensive behavior, it is possible to hypothesize that this neurotrophin may play a role in the mechanism of action of anabolic androgenic steroids (AAS) in the brain and be associated with endocrine and behavioral dysfunctions occurring due to AAS abuse. J. Neurosci. Res. 47:198–207, 1997. © 1997 Wiley‐Liss, Inc.
Impact of Shallow Trench Liner Oxidation Scheme on Junction Leakage

Shallow trench liner oxidation scheme is shown to have direct bearing on the reverse biased junction leakage. Experiments reducing liner oxide thickness and nitride undercut while increasing effective active area actually reduced the leakage currents by 40%. Silicided and unsilicided area, corner and perimeter intensive diodes all exhibited this behaviour. The perimeter component of leakage was most affected by liner oxidation scheme. Simulations of the oxidation process indicate a correlation between stress induced during the liner options and junction leakage.
Improving accuracy in the grading of renal cell carcinoma by combining the quantitative description of chromatin pattern with the quantitative determination of cell kinetic parameters.

The determination of grade and stage in renal cell carcinomas (RCCs) often fails to predict the actual clinical outcome for individual patients. The aim of the present work was to investigate whether it is possible to significantly improve the prognostic accuracy of the grading system by using the combination of two independent computer-assisted microscopy techniques. The first technique relates to the quantitative description of morphonuclear and nuclear DNA content features by means of the image analysis of Feulgen-stained cell nuclei, and the second quantitatively characterizes tumor growth by means of different cell kinetic parameters. These parameters consist of a duplication of a time-related parameter determined by means of the technique of using silver-stained proteins in interphase nucleolar organizer regions (AgNOR), a proliferation index determined by means of MIB-1 immunohistochemistry, and an apoptotic index determined by means of the terminal dUTP nick end labeling technique. The prognostic value of these quantitative features was investigated in a series of 60 RCCs. The quantitative analysis of Feulgen-stained nuclei made it possible to identify subgroups of patients with significantly different prognoses in both grade II and grade III RCCs. We labeled the RCCs associated with the most favorable prognoses as grade II- and III- and those with the least favorable ones as grade II+ and III+. The two most important kinetic variables to identify patients with different clinical outcomes were the MIB-1 index and the mean AgNOR area in the MIB-1-positive cells. Three significantly different survival curves were obtained for the 53 grade II and III RCC patients. Our results show that conventional RCC grading can be significantly improved by the quantitative analysis of Feulgen-stained nuclei, by cell kinetic parameter determination, and, more importantly, by combining the proliferation index with the mean AgNOR area parameter.
The pre-launch Planck Sky Model: a model of sky emission at submillimetre to centimetre wavelengths

We present the Planck Sky Model (PSM), a parametric model for generating all-sky, few arcminute resolution maps of sky emission at submillimetre to centimetre wavelengths, in both intensity and polarisation. Several options are implemented to model the cosmic microwave background, Galactic diffuse emission (synchrotron, free-free, thermal and spinning dust, CO lines), Galactic HII regions, extragalactic radio sources, dusty galaxies, and thermal and kinetic Sunyaev-Zeldovich signals from clusters of galaxies. Each component is simulated by means of educated interpolations/extrapolations of data sets available at the time of the launch of the Planck mission, complemented by state-of-the-art models of the emission. Distinctive features of the simulations are spatially varying spectral properties of synchrotron and dust; different spectral parameters for each point source; modelling of the clustering properties of extragalactic sources and of the power spectrum of fluctuations in the cosmic infrared background. The PSM enables the production of random realisations of the sky emission, constrained to match observational data within their uncertainties. It is implemented in a software package that is regularly updated with incoming information from observations. The model is expected to serve as a useful tool for optimising planned microwave and sub-millimetre surveys and testing data processing and analysis pipelines. It is, in particular, used to develop and validate data analysis pipelines within the Planck collaboration. A version of the software that can be used for simulating the observations for a variety of experiments is made available on a dedicated website.
The MTHFR C677T Polymorphism and Colorectal Cancer: The Multiethnic Cohort Study

Methylenetetrahydrofolate reductase (MTHFR) is a key regulatory enzyme in the metabolism of folate, a nutrient that has been inversely related to colorectal cancer risk. The common C677T variant in the MTHFR gene results in a reduced activity of this enzyme, thereby increasing the availability of folate for the production of thymidylate and purine for DNA synthesis and repair. We investigated the association of the 677TT genotype with colorectal cancer in a case-control study of 822 cases and 2,021 controls nested within the Multiethnic Cohort Study. The Multiethnic Cohort Study is a large prospective study of men and women of Japanese, White, African American, Latino, and Native Hawaiian origin, residing in Hawaii and Los Angeles. After adjusting for covariates, we found an inverse association between colorectal cancer risk and the TT genotype, with odds ratios (OR; and 95% confidence intervals) for the CC, CT, and TT genotypes of 1.00, 1.01 (0.84-1.21), and 0.77 (0.58-1.03), respectively. This association was similar in both sexes, stronger at high levels of folate intake, and limited to light and nondrinkers (P for interaction with ethanol = 0.02). An analysis by subsite (rectum versus colon) and stage (regional/distant versus in situ/localized) showed that the inverse association with the TT genotype was limited to colon tumors, especially those diagnosed at an advanced stage. The OR for the TT versus CC genotype for early- and late-stage colon cancer was 0.88 (0.58-1.33) and 0.52 (0.32-0.85), respectively (P for difference in OR = 0.04). The frequency of the T allele was relatively low in African Americans (0.13) and Native Hawaiians (0.22), consistent with their greater likelihood of presenting at a late stage when diagnosed with colorectal cancer. This study corroborates previous findings of an inverse association of the MTHFR 677TT genotype with colorectal cancer, especially at high levels of folate and low levels of ethanol intake. It also suggests that this effect may be specific to advanced colon cancer.
Adaptive Control for Stochastic Systems with Unknown Parameters and Input Delay

The adaptive dual control problem of a single-in single-out (SISO) stochastic system with unknown parameters and input delay is studied in this paper. The unknown parameters are assumed to be constant but belong to a known finite set. For each group of possible model parameters, a Kalman filter is used to estimate the system state online and the posterior probability of this model can also be calculated accordingly. The optimal control of each model is designed, based on an M steps ahead loss function. The final dual controller is obtained by a weighted sum of all possible optimal control outputs considering the posterior probabilities of each model. Numerical simulations were performed to verify the effectiveness of the proposed controller.
Transgenic mice and oncogenesis.

The creation of transgenic mice carrying specific cancer-promoting genes has opened an exciting new era in oncology. The biological effects of an individual oncogene on diverse cell types can now be assessed directly within the living animal. While transgenic animals bear the introduced oncogene in every tissue, expression of that gene may either be widespread or directed to a particular cell lineage, depending upon the regulatory sequences chosen. The transgene should behave identically in every animal of an established lineage and, indeed, perhaps in every cell of a given type. Thus, a well-characterized transgenic line becomes a permanent resource. Perhaps the most significant opportunity provided by these transgenic animals is the possibility of exploring the pre-neoplastic state. One can attempt to assess whether an oncogene has perturbed differentiation within particular lineages. The perturbations may help to delineate early matu­ ration stages and to clarify how differentiation is controlled. Thus, new insights may emerge regarding the normal biological functions of proto­ oncogenes. The rules for oncogene cooperativity can also be evaluated within diverse cell types. For example, one can isolate the relevant cells from a pre-neoplastic animal bearing an oncogene and attempt to trans­ form them fully in vitro with retroviruses carrying other oncogenes. Alter­ natively, the second oncogene could be introduced simply by breeding mice of two independent transgenic lines. While the study of transgenic oncogenes is still in its infancy, major new insights have already been gained. This chapter briefly summarizes how transgenic animals are produced, and then considers our current state of