Expressão do interferon-γ em biópsias gengivais de pacientes com periodontite crônica severa

O objetivo desse estudo foi analisar a expressao do interferon-gamma (INF-) em biopsias gengivais de sitios rasos e profundos de pacientes com periodontite cronica severa. O objetivo secundario foi correlacionar a expressao do INF- no fluido gengival com os sitios onde foram coletadas as biopsias gengivais. Foram coletadas biopsias de 22 pacientes portadores de periodontite cronica generalizada ou localizada severas (idade media 45,5  DP 8,9 anos), sendo 22 sitios profundos e 18 sitios rasos. O grupo controle foi composto por 14 pacientes clinicamente saudaveis (idade media 39,35  DP 16,5 anos). No total, foram 54 biopsias coletadas de 36 pacientes. As amostras do fluido gengival foram coletadas de alguns dos mesmos sitios de onde foram realizadas as biopsias, totalizando 12 sitios profundos, 8 sitios rasos e 4 sitios controle. Foram utilizados os parâmetros clinicos de avaliacao de profundidade de bolsa a sondagem (PB); nivel de insercao clinica (NIC); indice de placa visivel (IPV) e indice de sangramento gengival (ISG). O tecido foi removido com punch de 2 mm de diâmetro, na area cirurgica (grupo controle) ou na consulta para raspagem subgengival com ou sem acesso cirurgico (grupo teste) e armazenados em Eppendorffs com 1 ml de solucao de formaldeido a 10% para posterior analise morfologica e imuno-histoquimica. A intensidade da marcacao do INF- foi avaliada semiquantitativamente nas celulas epiteliais, plasmocitos, macrofagos, fibroblastos e celulas endoteliais, considerando-se marcacao forte (escore 2), marcacao fraca (escore 1) ou ausencia de marcacao (escore 0). O teste de Kruskal-Wallis foi utilizado para comparar a expressao do INF- nos tecidos epitelial e conjuntivo, entre os tres grupos (sitios profundos e rasos da periodontite e sitios controle). Observamos uma tendencia a um padrao de marcacao similar nos sitios rasos e profundos, com predominio de marcacao fraca nos sitios profundos. Nos sitios controle a marcacao do. (AU) The aim of this study was to analyze the expression of interferon-gamma (INF-γ) in gingival biopsies from shallow and deep sites in patients with severe chronic periodontitis. The secondary objective was to correlate the expression of INF-γ in the gingival crevicular fluid (GCF) with sites where the gingival biopsies were collected. Biopsies from 22 patients with chronic generalized or localized severe periodontitis (mean age 45.5 ± SD 8.9 years), 22 deep and 18 shallow sites were collected. The control group consisted of 14 patients clinically healthy (mean age 39.35 ± SD 16.5 years). In total, 54 biopsies were collected from 36 patients. The GCF samples were collected from some of the same sites where biopsies were performed, totaling 12 deep sites, 8 shallow sites and 4 control sites. Clinical evaluation parameters including probing pocket depth (PPD), clinical attachment level (CAL), visible plaque index (VPI) and gingival bleeding index (GBI) were used. The tissue was removed with a 2 mm of diameter punch, in the surgery area (control group) or in the subgingival scaling with or without surgical access (test group) and stored in an Eppendorff with 1 ml of 10% formaldehyde for subsequent morphological and immunohistochemical analysis. The intensity of staining was evaluated semi - quantitatively in the epithelial cells, plasma cells, macrophages, fibroblasts and endothelial cells, considering strong staining (score 2), weak staining (score 1) or absence of staining (score 0). The Kruskal-Wallis test was used to compare the expression of INF-γ in epithelial and connective tissues between the three groups (shallow and deep sites of periodontitis and control sites). We observed a trend for a similar pattern of staining in the shallow and deep sites, with a predominance of weak staining in deep sites. In control sites the staining of the epithelium was predominant. However, we could not demonstrate statistically significant differences between. (AU)