Miracle-dz/UniFAR-FTUs huggingface dataset:
Deep learning–based molecular morphometrics for kidney biopsies

Morphologic examination of tissue biopsies is essential for histopathological diagnosis. However, accurate and scalable cellular quantification in human samples remains challenging. Here, we present a deep learning-based approach for antigen-specific cellular morphometrics in human kidney biopsies, which combines indirect immunofluorescence imaging with U-Net-based architectures for image-to-image translation and dual segmentation tasks, achieving human-level accuracy. In the kidney, podocyte loss represents a hallmark of glomerular injury and can be estimated in diagnostic biopsies. Thus, we profiled over 27,000 podocytes from 110 human samples, including patients with anti-neutrophil cytoplasmic antibody-associated glomerulonephritis (ANCA-GN), an immune-mediated disease with aggressive glomerular damage and irreversible loss of kidney function. Previously unknown morphometric signatures of podocyte depletion were identified in patients with ANCA-GN, which allowed patient classification and showed potential for risk stratification in combination with routine clinical tools. Together, our approach enables robust and scalable molecular morphometric analysis of human tissues, yielding deeper biological insights into the human kidney pathophysiology. Summary Deep learning enables robust and scalable molecular morphometric analysis of human tissues, yielding deeper biological insights into the human kidney pathophysiology.
Hybrid coronary revascularization versus total arterial revascularization for the treatment of left main coronary artery disease

Background Hybrid coronary revascularization (HCR) has a similar clinical outcome to coronary artery bypass grafting (CABG) in treating multivessel disease. However, the outcome of HCR in treating left main coronary artery (LM) disease is unclear. This study sought to compare the clinical outcome of HCR with total arterial revascularization (TAR) for treating LM disease. Methods Patients who underwent treatment for LM disease in our center between January 2009 and December 2019 were selected. Of these, 33 patients underwent HCR, and 70 patients underwent TAR. The primary efficacy outcome of this study was mid-term major adverse cardiac and cerebrovascular events (MACCE). The primary safety outcome was perioperative MACCE. Results The incidence of postoperative outcomes was comparable between the two groups after adjustment with inverse probability weighting (IPW) (P>0.05). The median follow-up time was 47 (interquartile range, 20 to 85) months. There was no significant difference in the incidence of all mid-term outcomes and the freedom of MACCE between the two groups after adjustment (P>0.05). The Cox proportional hazard model demonstrated that HCR was not a significant determinant for MACCE [hazard ratio (HR) =3.516, 95% confidence interval (CI): 0.835 to 14.813]. Conclusions HCR may be safe and effective for the treatment of LM disease compared with TAR.
Perioperative Complications of Surgery for Degenerative Cervical Myelopathy: A Comparison Between 3 Procedures

Study Design: Retrospective comparative study. Objectives: To compare the perioperative complications of propensity score-matched cohorts of patients with degenerative cervical myelopathy (DCM), who were treated with anterior cervical discectomy and fusion (ACDF), posterior laminectomy with fusion, or laminoplasty. Methods: The Humana PearlDiver Patient Record Database was queried using the International Classification of Diseases (ICD-9 and ICD-10) and the Current Procedural Terminology (CPT) codes. Propensity score-matched analysis was done using multiple Chi-squared tests with Bonferroni correction of the significance level. Results: Cohorts of 11,790 patients who had ACDF, 2,257 patients who had posterior laminectomy with fusion, and 477 patients who had laminoplasty, were identified. After propensity score matching, all the 3 groups included 464 patients. The incidence of dysphagia increased significantly following ACDF compared to laminoplasty, P < 0.001, and in laminectomy with fusion compared to laminoplasty, P < 0.001. The incidence of new-onset cervicalgia was higher in ACDF compared to laminoplasty, P = 0.005, and in laminectomy with fusion compared to laminoplasty, P = 0.004. The incidence of limb paralysis increased significantly in laminectomy with fusion compared to ACDF, P = 0.002. The revision rate at 1 year increased significantly in laminectomy with fusion compared to laminoplasty, P < 0.001, and in ACDF compared to laminoplasty, P < 0.001. Conclusions: The incidence of dysphagia following laminectomy with fusion was not different compared to ACDF. Postoperative new-onset cervicalgia and revisions were least common in laminoplasty. The highest rate of postoperative limb paralysis was noticed in laminectomy with fusion.
Sleep does not aid the generalisation of binocular disparity‐based learning to the other visual hemifield

Visual perceptual learning refers to long‐lasting performance improvements on a visual skill – an ability supported by plastic changes in early visual brain areas. Visual perceptual learning has been shown to be induced by training and to benefit from consolidation during sleep, presumably via the reactivation of learning‐associated neuronal firing patterns. However, previous studies have almost exclusively relied on a single paradigm, the texture discrimination task, on which performance improvements may rely on higher‐order rather than lower‐level perceptual skills. In the present study, we tested whether sleep has beneficial effects on a visual disparity discrimination task. We confirm previous findings in showing that the ability to discriminate different disparities is unaffected by sleep during a 12‐hr retention period after training. Importantly, we extend these results by providing evidence against an effect of sleep on the generalisation of improved disparity discrimination across the vertical meridian. By relying on a between‐subject design, we further exclude carry‐over effects as a possible confound present in previous findings. These data argue against sleep as an important factor in the consolidation of a low‐level perceptual skill. This sets important constraints on models of the role of sleep and sleep‐associated neural reactivation in the consolidation of non‐declarative memories.
Facial Expression Ambiguity and Face Image Quality Affect Differently on Expression Interpretation Bias

We often show an invariant or comparable recognition performance for perceiving prototypical facial expressions, such as happiness and anger, under different viewing settings. However, it is unclear to what extent the categorisation of ambiguous expressions and associated interpretation bias are invariant in degraded viewing conditions. In this exploratory eye-tracking study, we systematically manipulated both facial expression ambiguity (via morphing happy and angry expressions in different proportions) and face image clarity/quality (via manipulating image resolution) to measure participants’ expression categorisation performance, perceived expression intensity, and associated face-viewing gaze distribution. Our analysis revealed that increasing facial expression ambiguity and decreasing face image quality induced the opposite direction of expression interpretation bias (negativity vs. positivity bias, or increased anger vs. increased happiness categorisation), the same direction of deterioration impact on rating expression intensity, and qualitatively different influence on face-viewing gaze allocation (decreased gaze at eyes but increased gaze at mouth vs. stronger central fixation bias). These novel findings suggest that in comparison with prototypical facial expressions, our visual system has less perceptual tolerance in processing ambiguous expressions which are subject to viewing condition-dependent interpretation bias.
Invasive trees rely more on mycorrhizas, countering the ideal weed hypothesis.

The ideal weed hypothesis predicts that invasive plants should be less dependent on mutualisms. However, evidence in favor or against this hypothesis comes mainly from observational studies. Here, we experimentally tested this hypothesis using a two-factor greenhouse experiment, comparing the seedling growth response of different Pinus species (varying in invasiveness) to ectomycorrhizal fungal inoculation. Most species showed no response until they were six months old, at which point inoculation increased growth between 10% and 260% among the different species. This growth response was higher for species with lower seed mass, higher dispersal ability, higher Z score (a proxy for invasiveness) and higher number of naturalized regions, all of which correspond to higher invasiveness. Our results show that timing is a crucial factor when comparing mycorrhizal dependecy of different species. Dependence on mutualistic microorganisms could be part of a strategy in which invasive species produce smaller seeds, in greater number, which can disperse further but where seedlings are more reliant on mycorrhizas to improve access to water, nutrients, and protection from pathogens. Our results suggest that reliance on mutualisms may enhance, rather than limit, non-native species in their ability to spread, establish and colonize.
Opioids and ophthalmology: review of the current literature

Purpose of review The number of opioid-related overdose deaths has rapidly increased since 2000, increasing more than five-fold from 1999 to 2016. Although surgeons only write 10% of opioid prescriptions annually, with ophthalmologists writing only a fraction of this amount, all physicians need to be cognizant of the current opioid epidemic and ways to decrease unnecessary opioid prescriptions. Recent findings Previous work within ophthalmology has shown that retrobulbar anesthesia along with peri-operative intravenous or oral nonopioid analgesics can lead to decreased postoperative opioid use following ophthalmic surgery. Recent literature has shifted focus towards the use of opioid prescription guidelines in managing postoperative pain and decreasing the number of unnecessary opioids being prescribed by ophthalmologists. Overall, the frequency of opioid prescriptions may have gradually declined the past few years with such efforts, increased awareness, and new healthcare policies to monitor opioid prescriptions. However, ophthalmologists still continue to prescribe a substantial number of opioid medications, much of which may not be necessary. Summary This review serves as a tool to aid all ophthalmologists in managing postoperative pain. There is a recent trend in addressing the opioid epidemic and efforts are being made to limit the overprescribing of opioids. Continued efforts are still required by all ophthalmologists to address the current opioid epidemic.
Decreased nutrient digestibility due to viscosity is independent of the amount of dietary fibre fed to growing pigs

Abstract Fibre content and its effect on chyme viscosity are associated with changes in the digestive system of humans and pigs. It is unclear if fibre content and viscosity affect digestive function independently or interactively. We evaluated apparent ileal digestibility (AID) of nutrients and intestinal function in thirty-six ileal-cannulated barrows fed for 29 d either maize–soyabean meal (MSBM) or high-fibre MSBM + 30 % distillers dried grains with solubles (MSBM + DDGS) modified to three levels of viscosity by adding 5 % non-viscous cellulose (CEL), 6·5 % medium-viscous carboxymethylcellulose (MCMC) or 6·5 % high-viscous CMC (HCMC). Digesta were collected on days 27 and 28 and intestinal samples on day 29. Feeding CMC, regardless of fibre content, increased viscosity of whole digesta (P = 0·003) and digesta supernatant (P < 0·0001) compared with CEL. Feeding MSBM + DDGS or CMC decreased AID of DM (P = 0·003; P < 0·0001) and crude protein (P = 0·02; P < 0·0001) compared with MSBM or CEL. Feeding CMC regardless of fibre content increased jejunal crypt depth (P = 0·02) and ileal goblet cell area (P = 0·004) compared with CEL. Adding DDGS or CMC did not affect villus height and gene expression of jejunal monosaccharide and amino acid transporters. Feeding HCMC, regardless of fibre content, elevated amylase activity by 46 and 50 % in jejunal (P = 0·03) and ileal digesta (P = 0·01) compared with CEL. In summary, diets with increased viscosity decreased nutrient digestibility and induced intestinal changes that were independent of the amount of fibre fed.
Spark breakdown of liquid hydrocarbons. I. Fast current and voltage measurements of the spark breakdown in liquid n‐hexane

A fast coaxial discharge line for the study of the electric breakdown of liquid hydrocarbons is described. It allows the simultaneous measurement of discharge current (up to 5 kA) and voltage (up to 50 kV) with nanosecond time resolution. The spark gap is of tip‐plane geometry. A detailed frequency analysis of the discharge and measurement circuits has been performed. Examples for the spark breakdown of n‐hexane under a variety of conditions are presented.
Surface initiated ATRP of acrylic acid on dopamine‐functionalized AAO membranes

A facile method for surface-initiated atom transfer radical polymerization (ATRP) on the anodic alu- minum oxide (AAO) membranes has been developed. The AAO membrane was firstly functionalized by poly (dopamine), the bromoalkyl initiator was then immobi- lized on the poly(dopamine) functionalized AAO mem- brane surface in a two-step solid-phase reaction, followed by ATRP of acrylic acid in a aqueous solution. The poly (acrylic acid) (PAAc)-grafted AAO membranes were char- acterized by X-ray photoelectron spectroscopy, fourier transform infrared spectroscopy and scanning electron microscopy. The XPS and FTIR results indicated that PAAc was successfully grafted on the AAO membrane surface. The degree of grafting increases linearly with the increase of monomer concentration, and it reaches a pla- teau when the reaction time up to 4 h. The results indi- cate that the thickness of the grafted polymer inside the isocylindrical pores of AAO membranes could be well controlled by changing the reaction time and monomer concentration. V C 2010 Wiley Periodicals, Inc. J Appl Polym Sci 117: 534-541, 2010
Sigh syndrome during the COVID-19 pandemic: Is it a signal of the mental health status of Chinese children and adolescents?

Background Since the outbreak of the COVID-19 pandemic, Chinese children and adolescents have been greatly affected by the strict social isolation policies, which will undoubtedly cause psychological problems. We aimed to investigate the mental health status of Chinese children and adolescents, and provided some considerations of the contributing factors and the coping strategy. Method We investigated the disease composition ratio of sigh syndrome in the pediatric outpatient clinic in Chengdu after the outbreak of the COVID-19 pandemic and outbreak mitigation. We also analyzed and compared the annual changing trends in the incidence of sigh syndrome in 2020 with those from previous years. Results The composition ratio of sigh syndrome increased significantly after the COVID-19 pandemic outbreak. The highest incidence was in March 2020, which then decreased gradually over the next few months, then increased again in June 2020 (P<0.05). And the 4–7 years old was the age group with a high incidence of sigh syndrome, and 5 years old had the highest incidence. Conclusions The mental health status of children and adolescents changed significantly after the outbreak of the COVID-19 pandemic. We should also pay attention to the psychological problems caused by returning to school after the epidemic has eased.
Real-world experience with pembrolizumab in patients with advanced soft tissue sarcoma

Background The goal of this study was to retrospectively analyze the efficacy and safety of pembrolizumab in the real-world treatment of soft tissue sarcoma (STS). Methods We analyzed 38 patients who suffered from STS and received pembrolizumab treatment from July 2017 to December 2018 in our hospital. We investigated the influence of clinical characteristics, treatment timing, and treatment protocol on objective response rate (ORR). We also investigated the factors affecting overall survival (OS) and progression-free survival (PFS), as well as the occurrence of severe adverse events (SAEs). Results The overall ORR was 19.4% (7/36). The ORRs of patients who received pembrolizumab treatment as first-line, second-line, and third-line therapy were 42.9% (3/7), 25.0% (4/16), and 0% (0/13), respectively, which showed marginal significance (P=0.052). Four patients (11.1%) maintained a complete response (CR) or partial response (PR) for at least 6 months with pembrolizumab monotherapy, or after withdrawal of chemotherapy or targeted therapy regimens. The median PFS was 2.9 months [95% confidence interval (CI): 2.4–3.4 months] and the median OS was 12.0 months (95% CI: 10.2–13.8 months). Cox regression analysis showed that treatment time was an independent factor affecting PFS (P=0.041), while Eastern Cooperative Oncology Group (ECOG) performance status (PS) score was the only independent factor affecting OS (P=0.028). Conclusions In the real world, the effectiveness of pembrolizumab in the treatment of STS was low. Some subtypes showed a limited response to pembrolizumab, including alveolar soft part sarcoma (ASPS), undifferentiated pleomorphic sarcoma (UPS), exoskeletal chondrosarcoma (ESCS), and angiosarcoma (AS), while the response in leiomyosarcoma (LMS) was low. Combination therapy may increase the risk of SAEs, especially when combined with pazopanib.
Automatic segmentation of pulmonary lobes on low-dose computed tomography using deep learning

Background To develop and validate a fully automated deep learning-based segmentation algorithm to segment pulmonary lobe on low-dose computed tomography (LDCT) images. Methods This study presents an automatic segmentation of pulmonary lobes using a fully convolutional neural network named dense V-network (DenseVNet) on lung cancer screening LDCT images. A total of 160 LDCT cases for lung cancer screening (100 in the training set, 10 in the validation set, and 50 in the test set) was included in this study. Specifically, the template of pulmonary lobes (the right lung consists of three lobes, and the left lung consists of two lobes) were obtained from pixel-level annotations by semiautomatic segmentation platform. Then, the model was trained under the supervision of the LDCT training set. Finally, the trained model was used to segment the LDCT in the test set. Dice coefficient, Jaccard coefficient, and Hausdorff distance were adopted as evaluation metrics to verify the performance of our segmentation model. Results In this study, the model achieved the accurate segmentation of each pulmonary lobe in seconds without the intervention of researchers. The testing set consisted 50 LDCT cases were used to evaluate the performance of the segmentation model. The all-lobes Dice coefficient of the test set was 0.944, the Jaccard coefficient was 0.896, and the Hausdorff distance was 92.908 mm. Conclusions The segmentation model based on LDCT can automatically and robustly and efficiently segment pulmonary lobes. It will provide effective location information and contour constraints for pulmonary nodule detection on LDCT images for lung cancer screening, which may have potential clinical application.
Human genetic admixture

Throughout human history, large-scale migrations have facilitated the formation of populations with ancestry from multiple previously separated populations. This process leads to subsequent shuffling of genetic ancestry through recombination, producing variation in ancestry between populations, among individuals in a population, and along the genome within an individual. Recent methodological and empirical developments have elucidated the genomic signatures of this admixture process, bringing previously understudied admixed populations to the forefront of population and medical genetics. Under this theme, we present a collection of recent PLOS Genetics publications that exemplify recent progress in human genetic admixture studies, and we discuss potential areas for future work.
Human papillomavirus vaccination for adults aged 30 to 45 years in the United States: A cost-effectiveness analysis

Background A nonavalent human papillomavirus (HPV) vaccine has been licensed for use in women and men up to age 45 years in the United States. The cost-effectiveness of HPV vaccination for women and men aged 30 to 45 years in the context of cervical cancer screening practice was evaluated to inform national guidelines. Methods and findings We utilized 2 independent HPV microsimulation models to evaluate the cost-effectiveness of extending the upper age limit of HPV vaccination in women (from age 26 years) and men (from age 21 years) up to age 30, 35, 40, or 45 years. The models were empirically calibrated to reflect the burden of HPV and related cancers in the US population and used standardized inputs regarding historical and future vaccination uptake, vaccine efficacy, cervical cancer screening, and costs. Disease outcomes included cervical, anal, oropharyngeal, vulvar, vaginal, and penile cancers, as well as genital warts. Both models projected higher costs and greater health benefits as the upper age limit of HPV vaccination increased. Strategies of vaccinating females and males up to ages 30, 35, and 40 years were found to be less cost-effective than vaccinating up to age 45 years, which had an incremental cost-effectiveness ratio (ICER) greater than a commonly accepted upper threshold of $200,000 per quality-adjusted life year (QALY) gained. When including all HPV-related outcomes, the ICER for vaccinating up to age 45 years ranged from $315,700 to $440,600 per QALY gained. Assumptions regarding cervical screening compliance, vaccine costs, and the natural history of noncervical HPV-related cancers had major impacts on the cost-effectiveness of the vaccination strategies. Key limitations of the study were related to uncertainties in the data used to inform the models, including the timing of vaccine impact on noncervical cancers and vaccine efficacy at older ages. Conclusions Our results from 2 independent models suggest that HPV vaccination for adult women and men aged 30 to 45 years is unlikely to represent good value for money in the US.
Antinociceptive effects of minor cannabinoids, terpenes and flavonoids in Cannabis

Cannabis has been used for centuries for its medicinal properties. Given the dangerous and unpleasant side effects of existing analgesics, the chemical constituents of Cannabis have garnered significant interest for their antinociceptive, anti-inflammatory and neuroprotective effects. To date, Δ9-tetrahydrocannabinol (THC) and cannabidiol (CBD) remain the two most widely studied constituents of Cannabis in animals. These studies have led to formulations of THC and CBD for human use; however, chronic pain patients also use different strains of Cannabis (sativa, indica and ruderalis) to alleviate their pain. These strains contain major cannabinoids, such as THC and CBD, but they also contain a wide variety of cannabinoid and noncannabinoid constituents. Although the analgesic effects of Cannabis are attributed to major cannabinoids, evidence indicates other constituents such as minor cannabinoids, terpenes and flavonoids also produce antinociception against animal models of acute, inflammatory, neuropathic, muscle and orofacial pain. In some cases, these constituents produce antinociception that is equivalent or greater compared to that produced by traditional analgesics. Thus, a better understanding of the extent to which these constituents produce antinociception alone in animals is necessary. The purposes of this review are to (1) introduce the different minor cannabinoids, terpenes, and flavonoids found in Cannabis and (2) discuss evidence of their antinociceptive properties in animals.