Miracle-dz/UniFAR-FTUs huggingface dataset:
Accommodating women's learning in continuing medical education

The purpose of this project was to present continuing medical education providers with a handbook that presents current perspectives on women's learning and suggests practice guidelines that can be incorporated into the planning of existing and future live continuing medical education activities. The context of the problem was to address the need to reduce gender-biased behavior in higher education classrooms. Research of the related literature led to the conclusion that, in continuing education, the educational needs of women are not being met or are inadequately addressed, because there is a general lack of understanding about women's learning in the continuing medical education community. To this end, The Continuing Medical Education Provider's Handbook for Accommodating Women's Learning1in Continuing Medical Education was developed and is recommended reading for all continuing medical education providers, their staff and faculty members. This handbook provides the tools needed to develop educational activities that accommodate women's learning.
Hispanic/Latino Patients with Gastric Adenocarcinoma Have Distinct Molecular Profiles Including a High Rate of Germline CDH1 Variants

Gastric cancer in Hispanic/Latino patients has unique genomic profiles that may contribute to the aggressive clinical phenotypes seen in these patients. Hispanic/Latino patients have a higher incidence of gastric cancer and worse cancer-related outcomes compared with patients of other backgrounds. Whether there is a molecular basis for these disparities is unknown, as very few Hispanic/Latino patients have been included in previous studies. To determine the genomic landscape of gastric cancer in Hispanic/Latino patients, we performed whole-exome sequencing (WES) and RNA sequencing on tumor samples from 57 patients; germline analysis was conducted on 83 patients. The results were compared with data from Asian and White patients published by The Cancer Genome Atlas. Hispanic/Latino patients had a significantly larger proportion of genomically stable subtype tumors compared with Asian and White patients (65% vs. 21% vs. 20%, P < 0.001). Transcriptomic analysis identified molecular signatures that were prognostic. Of the 43 Hispanic/Latino patients with diffuse-type cancer, 7 (16%) had germline variants in CDH1. Variant carriers were significantly younger than noncarriers (41 vs. 50 years, P < 0.05). In silico algorithms predicted five variants to be deleterious. For two variants that were predicted to be benign, in vitro modeling demonstrated that these mutations conferred increased migratory capability, suggesting pathogenicity. Hispanic/Latino patients with gastric cancer possess unique genomic landscapes, including a high rate of CDH1 germline variants that may partially explain their aggressive clinical phenotypes. Individualized screening, genetic counseling, and treatment protocols based on patient ethnicity and race may be necessary. Significance: Gastric cancer in Hispanic/Latino patients has unique genomic profiles that may contribute to the aggressive clinical phenotypes seen in these patients.
IκBα deficiency imposes a fetal phenotype to intestinal stem cells

The intestinal epithelium is a paradigm of adult tissue in constant regeneration that is supported by intestinal stem cells (ISCs). The mechanisms regulating ISC homeostasis after injury are poorly understood. We previously demonstrated that IκBα, the main regulator of NF‐κB, exerts alternative nuclear functions as cytokine sensor in a subset of PRC2‐regulated genes. Here, we show that nuclear IκBα is present in the ISC compartment. Mice deficient for IκBα show altered intestinal cell differentiation with persistence of a fetal‐like ISC phenotype, associated with aberrant PRC2 activity at specific loci. Moreover, IκBα‐deficient intestinal cells produce morphologically aberrant organoids carrying a PRC2‐dependent fetal‐like transcriptional signature. DSS treatment, which induces acute damage in the colonic epithelium of mice, results in a temporary loss of nuclear P‐IκBα and its subsequent accumulation in early CD44‐positive regenerating areas. Importantly, IκBα‐deficient mice show higher resistance to damage, likely due to the persistent fetal‐like ISC phenotype. These results highlight intestinal IκBα as a chromatin sensor of inflammation in the ISC compartment.
Resposta de Leucaena à inoculação comparada a diferentes tratamentos fertilizantes

The tree legume Leucaena leucocephala (Lam.) de Witt. , has a great potential for utilization in tropical agriculture, particularly in animal nutrition as an alternative protein source during dry periods, and in enriching the soil, both uses relying on its capacity for nitrogen fixation. With the aim of evaluating symbiotic nitrogen fixation in Leucaena an experiment was performed at the Instituto de Zootecnia, Nova Odessa, Sao Paulo, in cooperation with and following the experimental guidelines of the Niftal Project. The field trial was designed to test the effect of fertilization (lime + single super phosphate + KCl) and sources of nitrogen (inoculation, urea and control) in a 2 x 3 factorial design. The total nitrogen and dry matter production in kg.ha -1 –year -1 at the first cut were respectively: 106 ± 14 and 2416 ± 335, for fertilization + inoculation; 80 ± 14 and 1906 ± 316, for fertilization + urea; 70 ± 8 and 1798 ± 249, for fertilization; 67 ± 10 and 1902 ± 359, for inoculation; 29 ± 8 and 754 ± 193, for urea; and 50 ± 10 and 1039 ± 247 for the control. The statistical analysis showed significant responses to fertilization and/or inoculation, this latter being the best source of nitrogen in the experiment. The treatment with urea alone was the worst of all, suggesting the occurrence of acidification, which not only adversely affected the micro but also the macrosymbiont.
Clinical and biochemical profile of malaria: retrospective observational study from a tertiary centre

Background: Malaria is one of the common causes of acute febrile illness in tropical countries. Malaria presents with varied manifestations. This retrospective study carried to know the clinical profile and laboratory abnormalities seen in malaria patients. Methods: The data was collected retrospectively from 1 st January to 31 st December 2017. Inclusion criteria: all fever cases above 15 years of age of both the sexes diagnosed as malaria by peripheral smear examination and malaria card test. Exclusion criteria: combined malaria with other fevers such as dengue, chikangunya. Fever cases negative for malaria tests. Malaria cases with history of chronic kidney disease, chronic liver disease such as cirrhosis of liver, chronic viral hepatitis, liver abscess, and chronic illness such as rheumatoid arthritis, diabetes, and hypertension. The data regarding the clinical presentation of patients and laboratory values such as hemoglobin, total leukocytecount, platelet count, total bilirubin, SGOT, SGPT, albumin values collected and analyzed with tables and percentage. Results: A total of 57 malaria cases were analyzed, 71.9 % males, 28.1% were females. The commonest age group was between 15- 30 years (61.4%). 29 patients (50.9%) had P. vivax , 20 patients (35.1%) P. falciparum and 8 patients (14%) mixed infection. The most common clinical presentation was fever with chills (100%) followed by vomiting (68.4%), splenomegaly (56.1%), headache (45.6%), pain abdomen (43.9%). 19 cases (33.3%) had hemoglobin less than 10gm/dl; 42 cases (73.6%) had thrombocytopenia; 46 cases (80.7%) had urea ≥30mg/dl; 14 cases (24.6%) had creatinine ≥1.4; 26 cases (45.6%) had total bilirubin >1.2mg/dl ; 17 cases (29.8%) had SGOT >45 IU; 33 cases (57.9%) had SGPT > 45 IU and 32 cases (56.1%) had albumin level ≤3.5gm/dl. Conclusions: In the study malaria due to P. vivax was more common than P. falciparum , malaria affected young adults, males more than females. Reduced hemoglobin and platelet count, deranged liver and renal function and reduced serum albumin seen commonly in malaria.
Speciation and gene flow in two sympatric small mammals from Madagascar, Microgale fotsifotsy and M. soricoides (Mammalia: Tenrecidae)

Madagascar's shrew tenrecs (Mammalia: Tenrecidae; Microgale, Nesogale) represent an excellent system for studying speciation. Most species are endemic to the island's eastern humid forests, a region renowned for high levels of biodiversity and a high rate of in situ diversification. We set out to understand the speciation dynamics in a clade of recently described taxa: Microgale fotsifotsy and M. soricoides, which have nearly identical distributions in the moist evergreen forest, and M. nasoloi, which occurs in the western dry deciduous forest. A phylogenetic analysis using mitochondrial DNA data recovered two distinct clades of M. fotsifotsy: a south clade that is sister to, and broadly sympatric with, M. soricoides, and a north clade that is sister to the dry‐forest and distantly allopatric species M. nasoloi. To better understand this result, we analysed cranioskeletal measurements and performed demographic analyses using nuclear sequence data from ultraconserved elements. Nuclear data did not support a sister relationship between M. soricoides and the south clade of M. fotsifotsy but did demonstrate introgression between these clades, which probably explains the discordance between nuclear and mitochondrial phylogenies. Demographic analyses also revealed the absence of gene flow between the north and south clades of M. fotsifotsy. Morphometric data revealed several major differences between M. soricoides and M. fotsifotsy, as well as more subtle differences between the two clades of M. fotsifotsy. In light of these results, we treat the south clade of M. fotsifotsy as a new candidate species. Our findings demonstrate the utility of integrating multiple data types to understand complex speciation histories, and contribute to a growing body of evidence that species diversity on Madagascar is underestimated.
Effect of phosphorus application on 'Verano' Stylosanthes hamata and Centrosema pascuorum under sub-humid conditions in Nigeria

A study was conducted to evaluate diverse effects of phosphorus application on 2 forage legumes, viz 'Verano' Stylosanthes hamata and Centrosema pascuorum Mart. ex. Benth on an Alfisol under sub-humid conditions in northern Nigeria.The effect on dry-matter production and seed yield were significant.Applying P at 40 kg/ha was best for optimum production of Centrosema pascuornm. In fact, increasing P beyond this level reduced dry-matter yield of Centrosema pascuorum. Seed production was best at 60 kg P/ha for the legumes and increasing P above this leve reduced seed yield of 'Verano' Stylosanthes hamata. The application of P led to an increase in crude protein and P concentration of herbage,Crude protein and mineral composition of the forages declined with age and varied with changes in season. The highest value of the various nutrients were obtained at 8 weeks after sowing and during the wet season.
Identificação e caracterização de bactérias do ácido láctico isoladas de um produto cárneo fermentado tradicional e do ambiente fabril

Thirty six strains of L. plantarum, L. curvatus and L. sakei isolated from a traditional fermented meat product (chouriço) and workshop environment of two Portuguese production units, previously identified by biochemical tests, were genotypically identified using PCR and PCR Fingerprinting techniques. All strains were submitted to Antibiotic Sensibility Tests by disk diffusion method, using nine different antimicrobial substances. It was verified that a high percentage of strains were resistant to the antibiotic effects. All strains have not demonstrated lipolitic or proteolitic abilities. Lactic Acid Bacteria (LAB) are known by the production of inhibitory substances. The identified strains were evaluated for their ability to inhibit a sensitive indicator agent (E. avium EA5), cohabitant microorganisms (E. coli and S. xylosus) and pathogens (L. monocytogenes and S. aureus). Several strains have shown inhibitory capabilities to some of these agents. Two of them, L. plantarum PO5-15 and PO5-51, kept that skill even after centrifugation, liofilization and pH adjustment to 7,0, fact that enhances the possibility of production of bacteriocin-like substances or bacteriocins. These strains survived under pH 3,0 and Oxgall (1%) conditions, that reveals favorable characteristics for use as probiotic agents , with increased capability to pass through the stomach and intestine. Growth demonstrated by some strains when submitted to different temperature conditions (10oC, 25oC, 30oC and 44,5oC), allows their application in technological processes with different temperatures.
Gestão adequada de resíduos sólidos como fator de proteção na ocorrência da dengue

RESUMO Objetivo. Verificar a existência de associação de indicadores de gestão de resíduos sólidos e socioeconômicos municipais com índices de incidência de dengue, Zika e Chikungunya nos municípios do estado brasileiro de Minas Gerais. Métodos. Este estudo de caráter exploratório, quantitativo e transversal abrangeu os 853 municípios do estado de Minas Gerais. Todos os dados utilizados foram secundários, coletados e agrupados por regionais de planejamento. Como variáveis independentes, foram consideradas a cobertura de coleta de resíduos sólidos urbanos, cobertura de coleta seletiva e massa de resíduos sólidos urbanos, além de um indicador da qualidade da destinação final de resídulos, índices de desenvolvimento humano municipal e de Gini, renda mensal per capita e porcentagem de vulneráveis à pobreza. Os fatores potencialmente associados aos desfechos – incidências municipais de dengue, Chikungunya e Zika – foram selecionados inicialmente através de análises univariadas. Posteriormente, os modelos de regressão linear para as incidências de dengue, Chikungunya ou Zika foram gerados considerando os preditores selecionados pela análise univariada. Resultados. Não foi observada associação entre gestão de resíduos sólidos e incidência de Chikungunya e Zika. Por sua vez, a incidência de dengue associou-se à gestão de resíduos sólidos e apresentou relação inversa significativa com o percentual de vulneráveis à pobreza. Houve também associação direta o índice de Gini, sugerindo que quanto maiores os registros de incidência de dengue de 2007 a 2016, maiores os valores de Gini dos municípios – ou seja, maior a desigualdade social. A cobertura da coleta seletiva apresentou relação inversa e significativa com os casos de dengue, sugerindo que quanto menor a cobertura da coleta de seletiva, maiores foram os casos registrados de dengue. Conclusões. A gestão de resíduos sólidos pode influenciar os casos de dengue e, por isso, deve ser considerada nas ações de saúde pública.
Genetic and molecular analyses of mitochondrial inheritance and cytoplasmic male sterility in alfalfa.

Biparental inheritance of plastids has been demonstrated in approximately onethird of angiosperm species examined, although the species studied may not be representative of angiosperms in general. Biparental inheritance of mitochondria in angiosperms has been observed in only one case. Since essential cellular functions, as well as several important agronomic traits, are encoded by plant extranuclear genomes, biparental inheritance of organelles has important implications for plant genetics and breeding. Evidence of biparental inheritance of plastids in alfalfa (Medicago sativa L.) suggests the possibility that mitochondria may likewise be inherited biparentally. The objectives of this dissertation were to determine: 1) the inheritance of mitochondria in alfalfa (uniparental or biparental), and 2) the inheritance of cytoplasmic male sterility and the extent of male fertility restoration in single and population crosses of alfalfa. Several large RNA molecules observed in alfalfa mitochondrial preparations were inherited biparentally. These molecules were unaffected by RNase A added to preparations of intact mitochondria indicating that the RNA's were contained within an RNase impermeable compartment. Linear sucrose gradient purification failed to separate the RNA's from mitochondria and examination of sucrose gradient fractions using transmission electron microscopy (performed by J.K. Brown, Dept. of Plant Pathology, Univ. of Arizona) revealed that mitochondrial preparations were free of contamination by virus-like particles and other organelles. These results indicated that the large RNA's were contained within the mitochondrion. The inheritance of large mitochondrial RNA's in alfalfa provided evidence that mitochondria are inherited biparentally in this species. 9 Association of cytoplasmic male sterility with a particular organelle has not been determined in alfalfa, although the mitochondrion has been implicated in several other species. Analysis of progeny from single and population crosses provided evidence of biparental inheritance of cytoplasmic male sterility. Biparental cytoplasmic inheritance as well as nuclear inheritance may influence male fertility
21 Translational Control of Ferritin Synthesis

REGULATION OF IRON HOMEOSTASIS Iron is an important nutrient in mammalian cells, and the processes of uptake and sequestration of iron are highly regulated to ensure that supplies are adequate and toxicity is controlled. A major mediator of iron uptake is the transferrin receptor, a plasma membrane protein that binds serum diferric transferrin at the cell surface. Upon transferrin binding, the transferrin receptor– diferric transferrin complex is internalized in endosomes; within endosomes, iron is released from transferrin and transported into the cytosol and other compartments of the cell such as the mitochondria. When iron levels in the cell are high, toxic products generated by the reaction of iron with oxygen species can oxidize and damage cellular proteins, lipids, and nucleic acids (Halliwell and Gutteridge 1992). To protect against iron toxicity, cells synthesize ferritin, a 24-subunit spherical protein that sequesters as many as 4,500 iron atoms within precipitates in its interior (Theil 1987). Although ferritin serves a useful purpose in cells with high cytosolic iron levels, excess production of ferritin can be deleterious in iron-depleted cells because it may compete with other proteins for the limited amounts of available iron (Picard et al. 1998). Translational regulation of ferritin expression is the mechanism utilized by mammalian cells to ensure that ferritin is expressed primarily when it is needed for sequestration of excess iron (Fig. 1). Binding of cytosolic iron-sensing proteins known as iron regulatory proteins (IRPs) to a conserved RNA stem-loop element in the ferritin transcript interferes with ferritin translation in iron-depleted.
Animal-Assisted Interactions to Alleviate Psychological Symptoms in Patients Receiving Mechanical Ventilation

Mechanical ventilation is a common life-support intervention for critically ill patients that can cause stressful psychological symptoms. Animal-assisted interactions have been used in a variety of inpatient settings to reduce symptom burden and promote overall well-being. Because of the severity of illness associated with critical care, use of highly technological equipment, and heightened concern for infection control and patient safety, animal-assisted interaction has not been widely adopted in the intensive care unit. This case study of the therapeutic interaction between a canine and a mechanically ventilated patient provides support for the promotion of animal-assisted interactions as an innovative symptom management strategy in the intensive care unit.
Criopreservación postmortem de material espermático e inseminación artificial en el ciervo ibérico

El presente trabajo se planteo con un doble objetivo. En primer lugar, para evaluar de forma comparativa el efecto crioprotector de tres diluyentes sobre las caracteristicas a la postdescongelacion de las muestras espermaticas de alta (ACI) y baja calidad (BCI) inicial obtenidas postmortem en el ciervo. Y en segundo lugar, para determinar las posibilidades fecundantes reales de las dosis espermaticas asi congeladas mediante la realizacion de una prueba in vivo de inseminacion artificial. Los tres diluyentes utilizados en el primer experimento fueron: Citrato sodico-fructosa, Tris-lactosa y Triladil®. Las muestras espermaticas fueron recogidas del epididimo despues de la muerte de los venados. Para evaluar los efectos de estos tres diluyentes sobre las caracteristicas espermaticas se determinaron el porcentaje de movilidad individual (MI), la tasa de acrosomas intactos (AI) y el porcentaje de espermatozoides con endosmosis positiva (E+). La evaluacion de las muestras espermaticas reflejo diferencias estadisticamente significativas (p<0,001) en funcion del diluyente empleado y de la interaccion diluyente- calidad inicial de las mismas. Asi, para las dosis ACI los mayores valores (p<0,001) de MI, AI y E+ a la descongelacion se obtuvieron para el diluyente a partir de lactosa. Por el contrario, para las dosis BCI, fue el diluyente Triladil® el que mejores valores de calidad espermatica postdescongelacion reporto. En el segundo experimento, un grupo de 17 ciervas ibericas fue inseminado con dosis congeladas con Triladil® debido a que eran muestras BCI. Para ello, inicialmente se sincronizo la ovulacion mediante la aplicacion intravaginal de 2 CIDRs (0,33 g de Progesterona cada uno), inyectandose 300 UI de eCG en el momento de la retirada de los dispositivos. Los animales se inseminaron dos veces (a las 44 y 66 h de la retirada de los CIDRs) por via vaginal. Los resultados de fertilidad se determinaron sobre la base de los datos de los partos. De las 17 ciervas inseminadas, 4 (23,5 p.100) quedaron gestantes y parieron a los 235±4 dias de la inseminacion artificial.
Privacy-preserving Metrics for an mHealth App in the Context of Neuropsychological Studies

: The potential of smart devices as smartphones, smart watches and wearables in healthcare and rehabilitation, so-called mHealth applications, is considerable. It is especially interesting, that these devices accompany patients during their normal life. Hence they are able to track activities and support users in activities of daily life. But beside the beneﬁts for patients, mHealth applications also constitute a considerable privacy and security risk. The central question investigated here is how data about the usage of mobile applications in empirical studies with mHealth technologies can be collected in a privacy-friendly way based on the ideas of Privacy by Design. The context for the proposed approach are neuropsychological studies where a mobile application for Goal Management Training, a therapy for executive dysfunctions, is investigated. There a privacy-friendly concept for collecting data about the usage of the app based on metrics which are derived from research questions is proposed. The main ideas underlying the proposed concept are a decentralized architecture, where only aggregated data is gathered for the study, and a consequent data minimization approach.
Esfenvalerate および fenvalerate の妊娠ラットにおける胎盤透過性

酸側14C標識 esfenvalerate [(S)-α-cyano-3-phenoxybenzyl (S)-2-(4-chlorophenyl) isovalerate] と fenvalerate [(RS)-α-cyano-3-phenoxybenzyl (RS)-2-(4-chlorophenyl) isovalerate] をそれぞれ妊娠13日後のラットに2. 5または10mg/kg/dayの割合で1回経口または5日間連続経口投与を行なった. 両化合物ともに, 母獣血, 胎盤が胎仔, 羊水に比べて高い14Cレベルを示した. 両化合物および代謝物の胎仔への移行性は低く, 胎仔に移行した14C量は投与14C量の0. 07%以下であった. 両化合物間で胎仔14Cレベルおよび14C組織移行性 (14C組織レベル/14C母獣血レベル) には顕著な差は認められなかった. 胎仔, 母獣血, 胎盤中での主要14C化合物は両化合物ともに親化合物, 2-(4-chlorophenyl) isovaleric acid およびその水酸化体であり, fenvalerate からのみ cholesteryl (2R)-2-(4-chlorophenyl) isovalerate (CPIA-cholesterol ester) が母獣血と胎盤で痕跡量検出されたこと以外は両化合物間で差異はなかった. CPIA-cholesterol ester は母獣血から胎仔へは移行しないと考えられた. 以上より esfenvalerate と fenvalerate は胎盤透過性に関して同様な挙動をとることが判明した.