Miracle-dz/UniFAR-FTUs huggingface dataset:
ATGCTCGCCTACTGCGAGGCTCGACCCGAG CTCGGGTCGAGCCTCGCAGTAGGCGAGCAT GAGCCCATGATCCTGGGTACTCGGGATGAC GTCATCCCGAGTACCCAGGATCATGGGCTC CAGCACAGCCGAGAGGATGACCTGCCAACC GGTTGGCAGGTCATCCTCTCGGCTGTGCTG CCCCCTTCTGTTGAGAAGCCCAACCTGAGC GCTCAGGTTGGGCTTCTCAACAGAAGGGGG TGGGAAGGGCCAGCCAACGAGCAACCTGAC GTCAGGTTGCTCGTTGGCTGG

Article Chronology: Received 5 February 2009 Revised version received 29 May 2009 Accepted 14 June 2009 Available online 18 June 2009 Podosome formation in vascular smooth muscle cells is characterized by the recruitment of AFAP110, p190RhoGAP, and cortactin, which have specific roles in Src activation, local down-regulation of RhoA activity, and actin polymerization, respectively. However, the molecular mechanism that underlies their specific recruitment to podosomes remains unknown. The scaffold protein Tks5 is localized to podosomes in Src-transformed fibroblasts and in smooth muscle cells, and may serve as a specific recruiting adapter for various components during podosome formation.We show here that induced mislocalization of Tks5 to the surface of mitochondria leads to a major subcellular redistribution of AFAP-110, p190RhoGAP, and cortactin, and to inhibition of podosome formation. Analysis of a series of similarly mistargeted deletion mutants of Tks5 indicates that the fifth SH3 domain is essential for this recruitment. A Tks5 mutant lacking the PX domain also inhibits podosome formation and induces the redistribution of AFAP-110, p190RhoGAP, and cortactin to the perinuclear area. By expressing a catalytically inactive point mutant and by siRNA-mediated expression knock-down we also provide evidence that p190RhoGAP is required for podosome formation. Together our findings demonstrate that Tks5 plays a central role in the recruitment of AFAP-110, p190RhoGAP, and cortactin to drive podosome formation. © 2009 Elsevier Inc. All rights reserved.
Assessment of the Microbiological Quality of Vegetable from Urban Community Gardens in Korea

Many community gardens in large cities worldwide grow vegetables; however, no information regarding the levels of sanitary indicator bacteria and prevalence of foodborne pathogens in vegetables grown in urban community gardens is available. To evaluate the microbiological quality of vegetables from urban community gardens in Korea, 530 samples (nine types of vegetable, including Chinese cabbage, lettuce, radish leaves, spinach, mustard leaves, crown daisy, leek, Korean cabbage, and chicory) were collected at 11 urban community gardens in Seoul, Korea from September through October 2012. The levels of total aerobic bacteria, Escherichia coli, total coliforms, Salmonella spp. Listeria monocytogenes, and E. coli O157:H7 were evaluated quantitatively and/or qualitatively. The mean numbers of total aerobic bacteria and coliforms were 6.3 log CFU/g (range 3.8-8.1 log CFU/g) and 4.3 log CFU/g (range 2.1-6.4 log CFU/g), respectively. Total coliforms were detected on 67% of whole vegetables. Chicory showed the highest number of total aerobic bacteria and coliforms, whereas the lowest number of coliforms was detected on leeks. E. coli was detected on 2.3% of whole vegetables, including lettuce, radish leaves, mustard leaves, and chicory; however, foodborne pathogenic bacteria were not detected on any of the vegetable samples using this highly sensitive and validated procedure. Based on these findings, the presence of coliforms and E. coli demonstrates that opportunity for improvement of microbiological safety exists throughout the produce production chain, although no major foodborne pathogens were present in vegetables grown in urban community gardens.
THE CENTRALITY OF CRITICAL THINKING

AUTHOR Pierce, Gloria TITLE The Centrality of Critical Thinking in Educating for Diversity. PUB DATE Aug 93 NOTE 15p. ; Paper presented at the Annual International Conference on Critical Thinking and Educational Reform (13th, Sonoma, CA, August 1-4, 1993) and at the Annual Conference on Critical Thinking as an Educational Ideal (5th, Montclair, NJ, October 22, 1992). PUB TYPE Reports Evaluative/Feasibility (142)
Geography Matters: International Trade Patterns and the Indirect Land Use Effects of Biofuels

This article investigates the relationship between international trade patterns and the global distribution of coarse grain production responses to market developments in the United States. Our null hypothesis is that world markets are fully integrated, rendering the geographic persistence of bilateral trade flows irrelevant in the global production response to a change in U.S. prices. The alternative hypothesis allows price transmission to vary along with the intensity of competition among countries in specific markets. Using data from 1975 to 2002, we reject the null hypothesis. Our work has direct implications for the analysis of the global land use impacts of biofuel mandates. Copyright 2011, Oxford University Press.
Protein, Calcium, Zinc, and Iron Contents of Finger Millet Grain Response to Varietal Differences and Phosphorus Application in Kenya

This study was carried out to investigate the influence of phosphorus fertilizers on the concentrations of nutrients, particularly calcium, protein, zinc, and iron in finger millet grains grown in different agro-ecologies in Kenya. The on-station experiments were carried out at Kiboko (Eastern Kenya), Kakamega, and Alupe (Western Kenya) in 2015 during the short and long rainy seasons. The trials were laid out in a randomized complete block design (RCBD) in a 4 × 3 factorial arrangement with three replicates. The treatments comprised of four levels of phosphorus (0, 12.5, 25.0 and 37.5 kg ha−1 P2O5) and three finger millet varieties (U-15, P-224 and a local variety). Application of phosphorus significantly (p ≤ 0.05) increased the protein content of finger millet grain in varieties in all the three sites. Variety U-15 had the highest protein content (11.0%) at 25 kg ha−1 P2O5 with the control (zero P) on variety P-224 eliciting the lowest (4.4%) at Kiboko. At Kakamega, the 25 kg ha−1 P2O5 treatment with U-15 variety had the highest protein content (15.3%) while the same variety at 12.5 kg ha−1 P2O5 rate elicited the highest protein content (15.0%) at Alupe. Phosphorus application significantly enhanced the nutritional quality of finger millet grains specifically protein, calcium, iron, and zinc. Variety P-224 had the highest calcium content in all sites and highest iron content at Kakamega while the local varieties had the highest zinc content in all sites. The varieties responded differently to each quality component but generally, based on the protein content, the 25 kg ha−1 P2O5 is recommended.
Inventory of Ammonia Emissions from Livestock Production in Los Lagos and Los Ríos Regions, Chile

This paper presents the first inventory of ammonia emissions from livestock production in Los Lagos and Los Rios Regions of Chile. The inventory was focused in this area because is where cattle production is concentrated. This study aimed to quantify the amount of N losses due to ammonia volatilization from livestock production in these regions of the country, and to provide its spatial representation identifying their main sources. Calculations have been carried out for 1997 and 2007, and also projections to 2017 and 2027 were obtained. Calculated emissions were 6097 and 6206 t NH3-N for 1997 and 2007, respectively. Major sources of NH3-N emissions were cattle accounting for more than 85%, followed by horses and pigs. Farm management practices as grazing, housing, manure storage and land spreading of manure accounted for 87%, 9%, 3%, and 2%, respectively, of total annual emissions. Projections suggest that emissions could increase up to 6344 and 6516 t NH3-N for 2017 and 2027, respectively. Emissions are much lower than those reported for developed European countries. However, intensification of cattle production systems may lead to an increase of ammonia emissions which could impact the temperate forest of Southern Chile characterized by low nutrient fluxes.
Diverse clinical processes of 16 COVID-19 cases who concentrated infection in the same workplace in Beijing, China

Abstract Since December 2019, an outbreak of COVID-19 sweeping the world. Understanding the clinical and SARS-CoV-2 dynamic changes of mild and ordinary patients of COVID-19, so as to provide basis for the prevention and control of COVID-19. On February 1st, 2020, 16 SARS-CoV-2 RNA positive patients diagnosed in the same site in Beijing. The patients symptoms, signs, medication, and SARS-CoV-2 results were recorded. Of the 16 patients, 12 were female. Although they were infected at the same time in the same workplace, their clinical processes were very different and can be roughly divided into three different types: persistent sputum positive, persistent stool positive and persistent both positive. In 7 patients with mild clinical manifestations, the median days of SARS-CoV-2 RNA negative conversion in sputum samples were significantly later than those with obvious lung injury (27 days [range: 18 to 36]; 17 days, [range 6 to 25], P = .021). The negative conversion of SARS-CoV-2 RNA in stool was significant later than in sputum. There were various clinical manifestations after SARS-CoV-2 infection, even if they were infected by the same source of infection in the same place. The presence of SARS-CoV-2 virus RNA in stool samples was longer than that in respiratory tract.
Counterfactual analysis in R: a vignette

The R package Counterfactual implements the estimation and inference methods of Chernozhukov et al. (2013) for counterfactual analysis. The counterfactual distributions considered are the result of changing either the marginal distribution of covariates related to the outcome variable of interest, or the conditional distribution of the outcome given the covariates. They can be applied to estimate quantile treatment effects and wage decompositions. This vignette serves as an introduction to the package and displays basic functionality of the commands contained within.
Energy usage and benefit-cost analysis of cotton production in Turkey

The aim of this research is to determine the energy input and output involved in cotton production in the Hatay province of Turkey. The average energy consumption of the farms investigated in this study is 19 558 MJha-1. Of the total energy, 2.87% is direct and 71.13% is indirect. Renewable energy accounts for 12.30% and energy usage efficiency is found to be 2.36. The total energy input into the production of one kilogram of average Turkish cotton is estimated to be 4.99 MJ. The dominant contribution to input is energy in the form of nitrogen fertiliser (40.28%), followed by water for irrigation (22.37%) and diesel-oil (17.04%). The cost of cotton production per hectare is found to be 2 246 $ha-1 in the region, with 79.87% of this being variable costs. It can be concluded that intensive cotton farms are being operated in the area since the variable cost ratio is quite high. As a result of benefit-cost ratio (1.24) analysis, cotton production is found to be economically efficient. Key words: Cotton, input-output analysis, energy analysis, production economics, Turkey.
All Ears: Adults' and Children's Earwitness Testimony

Many crimes are committed under conditions of darkness, by masked perpetrators or over a phone. In such cases the witnesses’ auditory observations may have a vital role in the investigative phase and in court. Nevertheless, earwitness testimony is a neglected research area. The present thesis investigated earwitnesses’ (i) identification performance for an unfamiliar voice, (ii) memory for the perpetrator’s statement, and (iii) ability to describe the voice. All four studies used the same general setup; exposure to an unfamiliar voice for 40 seconds, and an interview including a seven-voice lineup after a two week delay. High ecological validity was a specific aim across all studies. Study I explored the performance of children aged 7–9 (N = 95), 11–13 (N = 78), and adults (N = 91). Half were exposed to a Target-Present lineup (TP), and half to a Target-Absent lineup (TA). For both types of lineups the participants performed poorly. In the TP condition only the 11–13-year olds (27 % correct) performed above chance level. Furthermore, in the TA condition, all age-groups showed a high willingness to make an identification. Study II investigated the influence of presentation format (direct vs. mobile phone recorded voices) on voice recognition accuracy. The participating adults (N = 165) were assigned randomly to one of the four conditions (Initial exposure: direct vs. mobile phone recorded voice; Lineup presentation: direct vs. mobile phone recorded voices). The overall accuracy for correct identification was 13%, which is expected by chance. Further, the results did not reveal any significant effect of presentation format or lineup format. Study III compared three types of interviews intended to enhance witnesses’ voice memory, as well as content recall. Additionally, an interview protocol developed by the Swedish Security Service, for questioning people that have only heard the perpetrator, was evaluated. After exposure, 11–13-year-olds (N = 119) and adults (N = 93) were interviewed, and returned after two weeks for an additional interview and a lineup. Overall performance for correct identifications was poor (children: 20%, adults: 19%), and an interview shortly after the witnessed event did not seem to help. The Cognitive Interview (vs. the Swedish Security Service protocol) was found to be beneficial for recalling the content of a brief conversation. Study IV investigated the effect of the perpetrator’s tone of voice and time delay on voice recognition accuracy. Further, two types of voice description interviews intended to strengthen the encoding of the voice, were tested. Adults (N = 148) and 11–13-year-olds (N = 160) either heard the perpetrator speak in a normal tone both at encoding and in the lineup, or in an angry tone at encoding and in a normal tone in the lineup. Witnesses were then interviewed about the voice, either with global questions, or by rating voice characteristics. Half of the witnesses were presented with a lineup shortly after the interview and the others after two weeks. Overall, neither…
Internationalisation of Higher Education: Service Quality in Higher Education in Selected Southern Hemisphere Countries

Abstract The tertiary sector has over the last two decades developed from a predominantly inwards focussed industry serving public interest to an internationalised and commercially competitive industry. This fundamental change resulted in amongst others a drive to better understand the most prominent dimensions that impact on internationalisation, more particularly the expectations and experiences of students. Although some of these changes can be argued to be country specific and thus differentiated between markets, others are universal and impact on the overall industry. One of the latter changes is a pressure to consider and treat students as clients, introducing all the dynamics of service delivery and management. This paper considers the importance of service delivery related to the market positioning of academic institutions in Australia and South Africa with a particular focus on non-academic internationalisation activities. The main findings reveal that the most important variable in the non-academic category is an effective induction program.
Adrenal dysfunction Postoperative Hyperthyroidism Visceral Cataménial headache Ischemic Neuropathic Nociceptive Sleep disorders Miscellaneous Sleep apnea Dermatologic pruritus Restless leg syndrome Chronic fatigue syndrome Nocturnal myoclonus syndrome Lyme disease Obesity hypoventilation syndrome

The treatment of primary insomnia may be complex and clinically challenging. A comprehensive multidimensional evaluation with a thorough history and physical examination coupled with appropriate testing/imaging will facilitate development of a working diagnosis. Optimal treatment strategies of challenging cases typically involve interdisciplinary team approaches (including a sleep medicine specialist) providing multimodal approaches to treatment, including nonpharmacologic and pharmacologic strategies. A stepped care approach to treatment may serve as a useful guide for health care providers unfamiliar with sleep disturbance issues. Treatment plans based on sound medical judgment, clinical insight, and a thorough and global understanding of particular patient’s comorbid conditions may lead to optimal patient-specific/patient-focused/patient centered personalized care.
Ytterbium oxide has the same accuracy as chromic oxide for estimating variations of faecal dry matter output in dairy cows fed a total mixed ration at two feeding levels

Daily herbage intake by ruminants at grazing can be estimated from faecal output and herbage digestibility. Chromic oxide has long been used as an external marker to estimate faecal dry matter (DM) output. The aim of this experiment was to determine the performance of ytterbium oxide compared to chromic oxide as an external marker for estimating variations of faecal DM output in dairy cows. Both markers were compared on eight Prim’Holstein lactating dairy cows, of which four were fistulated. Cows were offered a total mixed diet based on maize silage either at 100% or 70% of ad libitum DM intake, according to a single 2 × 2 reversal design with 2 experimental periods of 3 weeks. Faecal recovery of markers was determined over the last five days of each period by total faecal collection or by faecal sampling only during milking times. The between-day and the within-day variability of faecal marker concentrations were also investigated, as well as the between-day variability of faecal marker concentrations during the transition between periods 1 and 2. Both markers over-estimated the actual faecal DM output due to an incomplete faecal recovery. The average faecal recovery was slightly greater for Yb than for Cr (0.93 vs. 0.89). Faecal recovery of Yb and Cr was unaffected by feeding level irrespective of the faecal collection method. Ytterbium showed similar between-day and within-day variability of faecal concentration as Cr during the experiment (steady-state period), and similar between-day variability of faecal concentration as Cr during the transition period (non steady-state period). We conclude that ytterbium oxide used as a digestive marker seems to have the same accuracy as chromic oxide for estimating daily faecal DM output variations in dairy cows fed a total mixed ration, at low and high feeding level. © 2010 Elsevier B.V. All rights reserved.
Cladia aggregata (lichen) from Brazilian northeast: chemical characterization and antimicrobial activity

A composicao quimica e a atividade antimicrobiana da Cladia aggregata (Sw.) Nyl. foram avaliadas. O acido barbatico, um depsideo obtido da C. aggregata, foi espectroscopicamente analisado e testado, bem como o extrato orgânico. o extrato e a substância purificada foram testadas contra 4 linhagens de Staphylococcus aureus multi resistentes. A estrutura do acido barbatico foi confirmada atraves de ensaios de CCD, CLAE, IV, RMN (1H;13C), UV e analise elementar (r). O biocromatograma mostrou a acao deste composto junto com outras substâncias contidas no extrato orgânico, sugerindo a acao sinergica, a CMI do acido barbatico mostrou o mesmo nivel de inibicao de outras substâncias liquenicas.
The soil effects on the grapevine root system in several vineyards of the Loire valley (France)

The effects of different soil types on grapevine root distribution are presented and discussed for Loire Valley (France) conditions. Field studies with Cabernet franc/SO 4 root-stock vines showed four main rooting patterns in different soil types. A statistical analysis was used to determine the influence of several physical soil factors on root distribution. The soil water supply appears to have a beneficial effect on the root system. Conversely the penetrometer soil strength, bulk density and hydromorphic conditions are unfavourable for root development
implications of different brood pouch structures in syngnathid reproduction

the evolutionary radiation of the family syngnathidae was accompanied by a diversification of structures involved in male parental care whose anatomical variations may signal differences in reproductive strategies, with increasing egg protection possibly affecting female investment in offspring or larvae quality at the end of the embryonic development phase. an analysis of egg numbers showed significant differences between syngnathids with and without marsupium, suggesting that the brood pouch, besides increasing protection to the male and its eggs, also introduced the ability to carry an additional number of offspring per pregnancy, from one or more females. curiously, even though larvae sizes do not significantly differ between the considered brooding structures (controlling for male length), seahorse larvae were smaller than expected, given the large volume of the pear-shaped eggs. these observations suggest that the sealed seahorse pouch, although allowing the transport of a great number of offspring and capable of multiple consecutive pregnancies, may impose serious functional constraints, namely those related with oxygenation of the developing larvae, thus explaining the increased egg surface together with the presence of specific salinity control mechanisms inside the marsupium.
On the evolutionary state of massive stars in transition phases in M33

The advanced stages of several high-mass stars are characterized by episodic mass loss shed during phases of instability. Key for assigning these stars a proper evolutionary state is to assess the composition and geometry of their ejecta alongside the stellar properties. We selected five hot LBV candidates in M33 to refine their classification, investigate their circumstellar environments and explore their evolutionary properties. Being accessible targets in the near-infrared, we conducted medium-resolution spectroscopy with GNIRS/GEMINI in the $K-$band to investigate their molecular circumstellar environments. Two stars were found to display CO emission, which was modeled to emerge from a circumstellar or circumbinary Keplerian disk/ring. The identification of the carbon isotope $^{13}$C and, for one of the two stars, a significantly low $^{12}$CO/$^{13}$CO ratio, implies an evolved stellar state. As both CO emission stars are highly luminous and hence do not undergo a red supergiant phase, we suggest that stripping processes and equatorial high-density ejecta due to fast rotation are responsible for the enrichment of the stellar surface with processed material from the core. A candidate B[e]SG displays an absorption CO profile, which may be attributed to a jet or stellar pulsations. The featureless infrared spectra of two stars suggest a low-density gas shell or dissipation of the molecule due to the ionizing temperature of the star. We propose spectroscopic monitoring of our targets to evaluate the stability of the CO molecule and assess the time-dependent dynamics of the circumstellar gas structures.