Miracle-dz/UniFAR-FTUs huggingface dataset:
The Highs and Lows of Blood Pressure: Toward Meaningful Clinical Targets in Patients With Shock

Objective:Measurement of blood pressure is fundamental for the management of patients in shock, yet the physiological basis and meaning of blood pressure measurements are complex and often not well understood. This article is in two parts: part 1 deals with the mechanical and physiological aspects of blood pressure and its measurement and part 2 deals with the role of changes in regional resistances in the determination of tissue perfusion and bedside approaches to management of shock. Data Source and Selection:This review is based on physiological principles from texts and experimental studies which elucidate some of the key principles. The views expressed are the author’s synthesis of the views of others and his own opinions. Data Synthesis:Arterial blood pressure is a major determinant of regional flow and is often used as a surrogate indicator of tissue perfusion, but in reality, it is a poor indicator of blood flow. Blood pressure is determined by cardiac output (total flow) and total vascular resistance. Distribution of flow for a given blood pressure is dependent on the relative values of resistances in different vascular beds. If this distribution of resistances were known, this would be the ideal guide to therapy. Unfortunately, regional resistances cannot be assessed in the clinical setting, and so we are left with blood pressure as a guide to therapy. Conclusions:This article discusses the implications of these points and explores factors that need to be taken into account when designing empiric trials to determine appropriate blood pressure targets for patients in shock. Even if well-studied empirically developed guidelines become available, it likely still will be important to individualize patient management and approaches for this are discussed too.
Defining the Pseudomonas aeruginosa SOS Response and Its Role in the Global Response to the Antibiotic Ciprofloxacin

ABSTRACT Pseudomonas aeruginosa infections can be virtually impossible to eradicate, and the evolution of resistance during antibiotic therapy is a significant concern. In this study, we use DNA microarrays to characterize the global transcriptional response of P. aeruginosa to clinical-like doses of the antibiotic ciprofloxacin and also to determine the component that is regulated by LexA cleavage and the SOS response. We find that genes involved in virtually every facet of metabolism are down-regulated in response to ciprofloxacin. The LexA-controlled SOS regulon identified by microarray analysis includes only 15 genes but does include several genes that encode proteins involved in recombination and replication, including two inducible polymerases known to play a role in mutation and the evolution of antibiotic resistance in other organisms. The data suggest that the inhibition of LexA cleavage during therapy might help combat this pathogen by decreasing its ability to adapt and evolve resistance.
CJK-7, a Novel Flavonoid from Paulownia tomentosa Triggers Cell Death Cascades in HCT-116 Human Colon Carcinoma Cells via Redox Signaling.

BACKGROUND Colon cancer is the second most common cancer to cause death worldwide. About half of colon cancers patients require adjuvant therapy to control relapse following surgical resection. Therefore, abolition of tumor cell progression using an effective chemotherapeutic agent holds a feasible approach to treat patients suffering from colon cancer. In the present study, we evaluated the effects of geranylated flavonoid CJK-7, isolated from Paulownia tomentosa on HCT-116 human colon carcinoma cells. MATERIALS AND METHODS The effects of CJK-7 as an active component on HCT-116 cells programmed cell death and its underlying molecular mechanism were examined by using MTT assay, morphological assessment, H2DCFDA staining, Fura-2AM staining, Hoechst-33342 staining, comet assay, Acridine orange staining, mitochondrial membrane potential (ΔΨm) assay and Western blot analyses. RESULTS AND CONCLUSION The results revealed that, CJK-7 was capable of inducing caspase-dependent cell death events in cancer cells. Moreover, it was involved in up-regulation of autophagy signaling as evidenced by enhanced expression of LC3I/II. We also noticed stimulated expression of endoplasmic reticulum stress markers and phosphorylation of c-Jun NH2-terminal kinase (JNK), which was associated with up-regulated expression of p53, PUMA, Atg5 and Beclin-1, and down-regulation of Bcl-2, stressing the interaction of ROS on the aforementioned signaling. Furthermore, exposure to ROS scavengers (N-acetyl-l-cysteine (NAC), and JNK-specific inhibitor SP600125) significantly reversed the effects of CJK-7 by down-regulating apoptosis and autophagy signatures in HCT-116 cancer cells. Collectively our findings clarify the ROS-dependent regulatory effect of CJK-7 on programmed cell death signaling events in HCT-116 cancer cells while depicting its virile pro-oxidant capacity.
Gender classification by LPQ features from intensity and Monogenic images

Gender is one of the most useful facial attributes which are detected from human face images. In this work, we introduce a new gender classification system based on features extracted by Local Phase Quantization (LPQ) operators from intensity and Monogenic images. More detailed, the LPQ features are obtained from the input image (the intensity one) and from three other Monogenic components in the feature extraction stage. In the classification stage, we employ the binary SVM classifier to predict the gender of the given test images. The comparisons among our experimental results upon two public databases, LFW and Groups dataset, and those of other systems show that the proposed system is comparable with state-of-the-art approaches when it attains competing accuracy rates (97.0% on LFW and 91.58% upon Groups dataset).
Human Autonomic Responses to Actual and Simulated Weightlessness

Orthostatic dysfunction occurs after exposure to microgravity, and is not completely understood. The authors developed a device for stimulating carotid baroreceptors to test the hypothesis that exposure to microgravity leads to impairment of arterial baroreflex mechanisms. Data obtained before and after two head‐down bedrest studies and before and after brief Space Shuttle missions indicate that baroreceptor‐cardiac reflex control is impaired by simulated or actual weightlessness. The authors speculate that arterial baroreflex derangements combine with blood volume reductions and increased venous compliance to provoke orthostatic hypotension after microgravity exposure. Altered baroreflex function after missions may result from autonomic neuronal plasticity that develops during missions secondary to changes of cardiopulmonary and arterial dimensions and consequent changes of autonomic sensory input proxies.
Limited differential mRNA inactivation in the atp (unc) operon of Escherichia coli

Individual subunits of ATP synthase, encoded by the eight genes of the atp operon (atpA through atpH), have been found to be synthesized at a 10-fold range in molar amounts (D.L. Foster and R.H. Fillingame, J. Biol. Chem. 257:2009-2015, 1982; K. von Meyenburg, B.B. Jorgensen, J. Nielsen, F.G. Hansen, and O. Michelsen. Tokai J. Exp. Clin. Med. 7:23-31, 1982). We have determined the functional half-lives at 30 degrees C of mRNAs transcribed from these genes either during constitutive expression in a partial diploid strain or after induced expression from a plasmid. Accurate decay kinetics of the relative mRNA levels were determined by monitoring the rates of synthesis of the individual ATP synthase subunits by radioactive pulse labeling at different times after blocking transcription initiation with rifampin. The mRNA transcribed from the atp operon was found to be inactivated about twice as fast as the bulk mRNA in E. coli. Exceptions are the mRNA from the promoter-proximal atpB gene, which was inactivated about three times as fast as the bulk mRNA, and atpC mRNA, the inactivation rate of which was comparable to that of the bulk mRNA. These moderate differences in the kinetics of functional decay explain only a minor part of the differences in expression levels of the atp genes. We conclude, therefore, that the individual atp mRNAs must be translated with widely different efficiencies. The present analysis further revealed that mRNA degradation is sensitive to heat shock; i.e., after incubation at 39 degrees C for 5 min followed by a shift back to 30 degrees C, the decay rate of the bulk mRNA was decreased by 30%.
Aquaporins are Unlikely to be Affected in Marked Ultrafiltration Failure: Results from a Computer Simulation

S30 D hypertonic peritoneal dialysis (PD) dwells, an initial reduction in the dialysate sodium concentration regularly occurs with dwell time, owing to sodium sieving. Minimum dialysate Na concentration is attained after approximately 80 – 120 minutes; after which, the dialysate Na again approaches the plasma Na concentration. This phenomenon is most likely a result of osmotic water transport through endothelial water channels aquaporin-1 (AQP-1), which reject solutes and thereby cause dilution of the dialysate over the first 2 hours of the dwell (1–5). In severe ultrafiltration failure (UFF), an apparent lack of sodium sieving is a frequent occurrence (6). Lack of sodium sieving has usually been taken as evidence of reduced water transport through aquaporins, conceivably due to malfunction of those molecular water channels (7,8). Multiple mechanisms underlie the phenomenon of sodium sieving (6). The provisions for the phenomenon are these:
Relationship between limb length discrepancy and load distribution across the sacroiliac joint—a finite element study

We assessed the relationship between leg length discrepancy (LLD) and the load distribution across the sacro‐iliac joint (SIJ). A finite element model of the spine–pelvis was developed with different amounts of LLD by increasing the length of the right femur in the model. Peak stresses and contact loads across the SIJ were computed for different amounts of LLD (1, 2, and 3 cm). The load and the peak stresses across the SIJ articular surfaces progressively increased with the increase in the LLD. Trying to offset the LLD surgically by lengthening of the short side, shortening or stunting the growth (epiphysiodesis) of the long side, or by shoe lifts should decrease the load across the SIJ and should theoretically decrease SIJ pain. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1577–1580, 2012
Association between B-lymphocyte membrane immunoglobulin and multiple members of the Src family of protein tyrosine kinases

Treatment of B lymphocytes with antibodies to membrane immunoglobulin (Ig) stimulates protein tyrosine phosphorylation. We have examined the phosphorylation in vitro of proteins associated with membrane Ig. The Src family protein tyrosine kinases p53/56lyn, p59fyn, and p56lck are associated with membrane Ig in spleen B cells and B-cell lines and undergo phosphorylation in vitro. The pattern of expression of Src family protein tyrosine kinases in B cells varied. Our studies suggest that multiple kinases can potentially interact with membrane Ig and that within any one B-cell type, all of the Src family kinases expressed can be found in association with membrane Ig. We also observed that the Ig-associated Ig alpha protein, multiple forms of Ig beta, and proteins of 100 and 25 kDa were tyrosine phosphorylated in vitro. The 100- and 25-kDa proteins remain unidentified.
What is clinical empathy?

Patients seek empathy from their physicians. Medical educators increasingly recognize this need. Yet in seeking to make empathy a reliable professional skill, doctors change the meaning of the term. Outside the field of medicine, empathy is a mode of understanding that specifically involves emotional resonance. In contrast, leading physician educators define empathy as a form of detached cognition. In contrast, this article argues that physicians’ emotional attunement greatly serves the cognitive goal of understanding patients’ emotions. This has important implications for teaching empathy.
Differential regulation of cardiac adrenomedullin and natriuretic peptide gene expression by AT1 receptor antagonism and ACE inhibition in normotensive and hypertensive rats.

OBJECTIVE To study the effects of long-term treatment with the type 1 angiotensin (AT1) receptor antagonist losartan and the angiotensin-converting enzyme (ACE) inhibitor enalapril, on cardiac adrenomedullin (ADM), atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) gene expression. METHODS Spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats were given losartan (15 mg/kg per day) or enalapril (4 mg/kg per day) orally for 10 weeks. The effects of drugs on systolic blood pressure, cardiac hypertrophy, ANP, BNP and ADM mRNA and immunoreactive-ANP (IR)-ANP, IR-BNP and IR-ADM levels in the left ventricle and atria were compared. RESULTS Losartan and enalapril treatments completely inhibited the increase of systolic blood pressure occurring with ageing in SHR. The ratio of heart to body weight was reduced in both losartan- and enalapril-treated SHR and WKY rats. Treatment with losartan or enalapril reduced left ventricular ANP mRNA and IR-ANP in both strains, and ventricular BNP mRNA levels in SHR rats. Inhibition of ACE, AT1 receptor antagonism, changes in blood pressure or cardiac mass had no effect on left ventricular ADM gene expression in SHR and WKY rats. In addition, atrial IR-ANP and IR-ADM levels increased in SHR whereas IR-BNP levels decreased in WKY and SHR rats in response to drug treatments. CONCLUSIONS Our results show that ventricular ADM synthesis is an insensitive marker of changes in haemodynamic load or cardiac hypertrophy. Furthermore, the expression of ADM, ANP and BNP genes is differently regulated both in the left ventricle and atria in response to AT1 receptor antagonism and ACE inhibition.
Associative memory design for the fast track processor (FTK) at ATLAS

We propose a new generation of VLSI processors for pattern recognition, based on associative memory architecture, optimized for online track finding in high-energy physics experiments. We describe the architecture, the technology studies and the prototype design of a new associative memory project: it maximizes the pattern density on the ASIC, minimizes the power consumption and improves the functionality for the fast tracker processor proposed to upgrade the ATLAS trigger at LHC.
Inclusion theorems of double Deferred Cesàro means II

Abstract In 1932 R. P. Agnew present a definition for Deferred Cesàro mean. Using this definition R. P. Agnew present inclusion theorems for the deferred and none Deferred Cesàro means. This paper is part 2 of a series of papers that present extensions to the notion of double Deferred Cesàro means. Similar to part 1 this paper uses this definition and the notion of regularity for four dimensional matrices, to present extensions and variations of the inclusion theorems presented by R. P. Agnew in [2].
Partial nephrectomy in mice with milliwatt carbon dioxide laser and its influence on experimental metastasis

We have developed a surgical model to perform partial nephrectomy in mice using the milliwatt CO2 laser and have used this model for studying the influence of the sequel of surgery on experimental tumor metastasis. Strain A mice were subjected to partial nephrectomy using the milliwatt CO2 laser. The surgical procedure was time efficient, the blood loss was minimal, and the postoperative mortality was 6%. Immediately after surgery, the wound consisted of a superficial layer of charring and a deeper layer of thermal damage (coagulative necrosis). The wound healing was completed within 30 days and was accompanied by fibroblast infiltration and tubular regeneration but minimal inflammatory response. Seventy surgical mice were injected I.V. with TA3Ha murine mammary adeno‐carcinoma cells at different intervals (immediately to 30 days) after surgery. Among 38 mice inoculated with tumor cells immediately or up to 3 days after surgery, 18 (47%) showed histologically confirmed tumors at the site of surgical trauma. None of the 38 unoperated kidneys showed any evidence of tumor. This difference is statistically significant at a P value of <0.001. As the interval between surgery and tumor inoculation was increased to 7, 15, and 30 days, the frequency of tumor formation at the site of surgery decreased to 20% (2/10), 14% (2/14), and 0% (0/8), respectively. The results demonstrate that a) partial nephrectomy in mice is feasible with minimal mortality or apparent morbidity, b) the laser‐induced surgical trauma favors implantation and growth of tumors, c) the frequency of tumor formation is related to the stage of wound healing, and d) the tumors are anatomically related to the healing wound but do not invade into the parenchymal tissue.
Conformational studies by 1H nuclear magnetic resonance of the basic pancreatic trypsin inhibitor after reduction of the disulfide bond between Cys-14 and Cys-38. Influence of charged protecting groups on the stability of the protein.

The disulfide bond Cys-14–Cys-38 in the basic pancreatic trypsin inhibitor was reduced and the cysteinyl side chains were protected with iodoacetamide, iodoacetic acid or ethyleneimine. At pH values near 5, the first of these protecting groups is neutral, the second negatively charged and the third positively charged. High-resolution 1H nuclear magnetic resonance showed that the average spatial structures of the three modified proteins are essentially identical. The influence of the different electrostatic charges on the stability and the internal flexibility of the proteins could thus be studied in a fixed spatial arrangement of the polypeptide chain. In 2H2O solution at p2H 5.3, the denaturation temperatures are 79°C for the carboxymethylated, 76°C for the carboxyamidomethylated and 70°C for the aminoethylated reduced inhibitor. Comparison of the three species indicates that stabilizing and destabilizing effects of the charged groups are mainly due to local interactions with nearby charged amino acid side chains, rather than to the variations of the overall charge of the proteins. The stabilizing or destabilizing effects per charged group were estimated to be of the order of 1.0–2.0 kJ mol-1. The different stabilities of the three proteins are also manifested in the amide proton exchange rates, whereas the internal mobilities of corresponding aromatic rings were found to be identical in the three species.
Morphometric study of the interproximal unit in the esthetic region to correlate anatomic variables affecting the aspect of soft tissue embrasure space.

BACKGROUND The presence of a normal papilla is crucial to avoid the unpleasant esthetic defects that are of major concern to periodontists, restorative dentists, and patients. During the course of progressive periodontitis and following periodontal treatment, it is not uncommon to have a partial loss of the interdental papilla. This loss can lead to an unesthetic gingival appearance. This study evaluated different anatomic variables in an effort to determine their role in the papillary appearance of maxillary incisors. METHODS A total of 178 interdental embrasures in 58 patients were selected randomly for examination. For each patient, a digital photograph and a modified periapical radiograph of the interdental embrasure of the four maxillary incisors were taken by using a special metric device fixed to a centrator as a reference marker. Clinical and radiographic data were obtained for the distance from the contact point to the alveolar crest and for the interradicular distance. We used a classification system with regard to peri-implant soft tissue based on esthetic assessments related to the space between reference lines through the highest gingival curvature of the crown-tooth margin and the contact point. RESULTS In the group of interdental sites with an interradicular distance of less than approximately 2.4 mm, an increase in the distance between the contact point and the bone crest corresponded to a marked increase in the interdental black triangle's dimensions and, therefore, a less esthetic smile. In particular, when the interradicular distance was >2.4 mm, we statistically estimated that the other anatomic variable considered, the distance from the contact point to the alveolar crest, lost its influence on whether the interdental papilla would be present or absent. CONCLUSION The interradicular distance and the distance between the contact point and the alveolar crest have independent and combined effects on the presence or absence of the interdental papilla.
Bacterial Growth in Mixed Cultures on Dissolved Organic Carbon from Humic and Clear Waters

Interactions between bacterial assemblages and dissolved organic carbon (DOC) from different sources were investigated. Mixed batch cultures were set up with water from a humic and a clear-water lake by a 1:20 dilution of the bacterial assemblage (1.0 μm of prefiltered lake water) with natural medium (sterile filtered lake water) in all four possible combinations of the two waters and their bacterial assemblages. Bacterial numbers and biomass, DOC, thymidine incorporation, ATP, and uptake of glucose and phenol were followed in these cultures. Growth curves and exponential growth rates were similar in all cultures, regardless of inoculum or medium. However, bacterial biomass produced was double in cultures based on water from the humic lake. The fraction of DOC consumed by heterotrophic bacteria during growth was in the same range, 15 to 22% of the total DOC pool, in all cultures. Bacterial growth efficiency, calculated from bacterial biomass produced and DOC consumed, was in the order of 20%. Glucose uptake reached a peak during exponential growth in all cultures. Phenol uptake was insignificant in the cultures based on the clear-water medium, but occurred in humic medium cultures after exponential growth. The similarity in the carbon budgets of all cultures indicated that the source of the bacterial assemblage did not have a significant effect on the overall carbon flux. However, fluxes of specific organic compounds differed, as reflected by glucose and phenol uptake, depending on the nature of the DOC and the bacterial assemblage.
Curve analog control of maximum torque per ampere for permanent magnet synchronous motor used in electric vehicles

This paper proposes a curve analog control of maximum torque per ampere control (MTPAC) for permanent magnet synchronous motor (PMSM) used in electric vehicles. Iterative delivery and the least square method are employed to derive the d-q-axis current component and the curve simulation of the data. Then the whole curve is divided into three sections whose highest index is two. Compared to the traditional MTPAC, the new one is more simple and practical. The advantages of the improved scheme are analyzed and verified by computer simulations. The experimental results show that the proposed scheme can guarantee the high efficient robust for the interior permanent magnet synchronous motor (TPMSM).
[The child and his family].

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