Miracle-dz/UniFAR-FTUs huggingface dataset:
Protective capacity and epitope specificity of CD8+ T cells responding to lethal West Nile virus infection

West Nile virus (WNV) is a small, positive‐strand RNA virus belonging to the Flaviviridae genus, which causes lethal encephalitis in a subset of infected birds and mammals. In humans, WNV exhibits pronounced age‐related morbidity and mortality, but the basis of this effect is unclear, and the molecular and cellular parameters of the host‐WNV infection are just beginning to be elucidated. Indeed, numerous mechanisms were implicated in protection in vivo against WNV (IFN‐I and IFN‐γ, antibody, C’, CD8 and CD4 T cells), but the individual importance of each one of these remains unclear. Here, we show that transfer of highly enriched naïve CD8+ T cells protects the majority of alymphoid mice against lethal WNV infection. To substantiate and expand this finding, we defined the peptide specificity of the CD8 response in H‐2b mice and used a panel of identified peptides to map one dominant (NS4b 2248–2256) and several subdominant epitopes. The hierarchy of these epitopes was stably maintained in the memory responses. Most importantly, CTL lines directed against these peptides conferred protection against lethal WNV infection in direct proportion to the epitope immunodominance. These results provide a springboard for future characterization of T cell responses against WNV and demonstrate, for the first time, that CD8 T cells can single‐handedly protect from this disease.
Effects of a lubricin-containing compound on the results of flexor tendon repair in a canine model in vivo.

BACKGROUND Tendon surface modification with a synthetic biopolymer, carbodiimide-derivatized hyaluronic acid and gelatin with the addition of lubricin (CHL), has been shown to reduce gliding resistance after tendon repair in an in vitro model. The purpose of the study was to investigate whether CHL would reduce adhesion formation and improve digital function after flexor tendon repair in a canine model in vivo. METHODS Sixty dogs were randomly assigned to either a biopolymer-treated group (n = 30) or an untreated control group (n = 30). The second and fifth flexor digitorum profundus tendons from each dog were lacerated fully at the zone-II area and then repaired. Passive synergistic motion therapy was started on the fifth postoperative day and continued until the dogs were killed on day 10, day 21, or day 42. The repaired tendons were evaluated for adhesions, normalized work of flexion, gliding resistance, repair strength, stiffness, and histological characteristics. RESULTS The normalized work of flexion of the repaired tendons treated with CHL was significantly lower than that of the non-CHL-treated repaired tendons at all time points (p < 0.05), and the prevalence of severe adhesions was also significantly decreased in the CHL-treated tendons at day 42 (p < 0.05). However, the repair failure strength and stiffness of the CHL-treated group were also significantly reduced compared with those of the control group at days 21 and 42 (p < 0.05) and the rate of tendon rupture was significantly higher in the treated group than in the control group at day 42 (p < 0.05). CONCLUSIONS Treatment with the lubricin-containing gel CHL appears to be an effective means of decreasing postoperative flexor tendon adhesions, but it is also associated with some impairment of tendon healing. Future studies will be necessary to determine if the positive effects of CHL on adhesion formation can be maintained while reducing its adverse effect on the structural integrity of the repaired tendon.
Biogas Potential of Coffee Processing Waste in Ethiopia

Primary coffee processing is performed following the dry method or wet method. The dry method generates husk as a by-product, while the wet method generates pulp, parchment, mucilage, and waste water. In this study, characterization, as well as the potential of husk, pulp, parchment, and mucilage for methane production were examined in biochemical methane potential assays performed at 37 °C. Pulp, husk, and mucilage had similar cellulose contents (32%). The lignin contents in pulp and husk were 15.5% and 17.5%, respectively. Mucilage had the lowest hemicellulose (0.8%) and lignin (5%) contents. The parchment showed substantially higher lignin (32%) and neutral detergent fiber (96%) contents. The mean specific methane yields from husk, pulp, parchment, and mucilage were 159.4 ± 1.8, 244.7 ± 6.4, 31.1 ± 2.0, and 294.5 ± 9.6 L kg−1 VS, respectively. The anaerobic performance of parchment was very low, and therefore was found not to be suitable for anaerobic fermentation. It was estimated that, in Ethiopia, anaerobic digestion of husk, pulp, and mucilage could generate as much as 68 × 106 m3 methane per year, which could be converted to 238,000 MWh of electricity and 273,000 MWh of thermal energy in combined heat and power units. Coffee processing facilities can utilize both electricity and thermal energy for their own productive purposes.
Prothrombotic Changes in Diabetes Mellitus

Abstract Although our understanding of vascular pathology has greatly improved in recent years, the cellular and molecular mechanisms underlying the enhanced thrombotic propensity in type 2 diabetes mellitus (T2DM) remain incompletely characterized. Detrimental interactions between activated vascular cells (i.e., platelets, leukocytes, endothelial cells) and the vulnerable atheromatous plaque are a major determinant of the increased atherothrombotic burden in T2DM patients. Endothelial damage and accelerated senescence, impairment of the endothelial progenitor cell repair system, plaque neovascularization and inflammation, decreased clearance of detrimental molecules within the plaque, and increased expression of matrix metalloproteinases may collectively contribute to intraplaque hemorrhage and subsequent rupture. Notably, recent data demonstrates the central importance of the tissue factor-microparticle–mediated pathway in diabetic thrombophilia and cardiovascular complications. Acting as detrimental amplifiers of various biological responses (including thrombogenicity and plaque remodeling), microparticles have also emerged as a key marker of global vascular damage in T2DM patients. Available evidence suggests that targeting the tissue factor-microparticle pathway may be a promising approach for reducing the burden of the atherosclerotic complications of diabetes.
An immunochemical, ultrastructural, and developmental characterization of the horizontal basal cells of rat olfactory epithelium

The olfactory epithelium, which retains a capacity for neurogenesis throughout life, contains two categories of basal cells, dark/horizontal and light/globose, neither of which is fully characterized with respect to their function during the processes of neurogenesis and epithelial reconstitution after injury. The aim of this study was to define the potential biological role(s) of dark/horizontal basal cells (D/HBCs) in the epithelium by performing immunochemical, electron microscopic, and developmental analyses of this cell population. The D/HBCs express several specific immunochemical characteristics, which include the rat homologues of human cytokeratins 5 and 14, which were identified on the basis of staining with subunit‐ specific monoclonal antibodies and two‐dimensional immunoblot analysis of the immunoreactive proteins. Indeed, the D/HBCs are the only cells in the olfactory mucosa that express these specific cytokeratins. The D/HBCs also express an α‐galactose or α‐N galactosamine moiety to which the Iß4 isolectin from Bandeiraea simplicifolia binds. Moreover, the D/HBCs are heavily labeled by two different antibodies against the EGF receptor and by a monoclonal antibody that binds to phosphotyrosine. These characteristics are also common to the basal cells of respiratory epithelium. The electron microscopic analysis of the basal region of the olfactory epithelium and the light microscopic immunofluorescence observations demonstrate that the D/HBCs provide a bridge between the basal processes of some sustentacular cells and the basal lamina. The most striking ultrastructural feature of the D/HBCa is their enfolding of virtually all bundles of olfactory axons within tunnels formed where D/HBCs arch over the basal lamina. The intimacy of the arrangement between D/HBCs and olfactory axons suggests that signals may pass from axons to D/HBCs or vice‐versa. With respect to the development of D/HBCs, cells that express cytokeratins 5 and 14 and the EGF receptor first appear near the boundary with respiratory epithelium late in development, but do not extend throughout the olfactory epithelium until the middle of the first postnatal week. Taken together, the present findings and previously published data suggest that D/HBCs help to maintain the structural integrity of the olfactory epithelium, participate in its recovery from injury, and may also function to signal the status of the neuronal population of the epithelium. © 1995 Wiley‐Liss, Inc.
Two species of human CRK cDNA encode proteins with distinct biological activities

Two distinct human CRK cDNAs, designated CRK-I and CRK-II, were isolated from human embryonic lung cells by polymerase chain reaction and by screening of a human placenta cDNA library, respectively. CRK-I differed from CRK-II in that it lacked a 170-nucleotide sequence, suggesting that CRK-I and CRK-II were the products of alternative splicing. The amino acid sequences deduced from these two cDNAs differed in the carboxyl termini and contained one SH2 and either one or two SH3 domains. RNAse protection analysis demonstrated both CRK-I and CRK-II mRNAs in various human cells. Three CRK proteins, of 42, 40, and 28 kDa, were identified in human embryonic lung cells by means of antibodies against the SH2 region and the SH3 region of the bacterially expressed CRK-I protein. Transient expression of CRK-I and CRK-II cDNAs in COS7 cells showed that the former encoded the 28-kDa protein and the latter encoded the 40- and 42-kDa proteins. All human cell lines so far examined expressed the 40-kDa protein; however, expression of the 28- and the 42-kDa proteins was variable. In a comparison of the biological activity of the two human CRK proteins, both proteins were stably expressed in rat 3Y1 cells. All cell lines expressing CRK-I protein showed altered morphology, proliferated in soft agar, and grew as massive tumors in nude mice. Although CRK-II-expressing cells showed a slight morphologic change, they did not make colonies in soft agar or grow in nude mice. These results demonstrate that the two species of human CRK cDNA encode proteins which differ in their biological activities.
Monensin controlled-release intraruminal capsule for control of bloat in pastured dairy cows.

Monensin, a polyether ionophore antibiotic, is potentially an important agent for bloat relief in dairy cows grazing temperate legume-based pasture. A series of studies was undertaken to determine the effect of monensin, when delivered continuously in the rumen of lactating dairy cows by means of controlled-release capsules (monensin CRC). Such devices release approximately 300 mg/head/day for 100 d. A short-term pilot study made at Ruakura, New Zealand, tested monensin CRC in cows selected for high susceptibility to bloat and grazing lucerne (Medicago sativa) or red clover (Trifolium pratense). Treatment significantly reduced the incidence of bloat, while milk yield and protein yield were increased. There was no effect on fat yield. Following the pilot study, 6 large-scale field experiments involving a total of 368 lactating dairy cows, were made in Australia and New Zealand to confirm the effectiveness of monensin CRC for bloat control and to measure the effect of such treatment on milk production and composition. A severe bloat problem occurred in 2 experiments, mild bloat occurred in 2 others, while no visual signs of bloat were observed in the remaining 2 experiments. Bloat was significantly (P less than 0.05) reduced by monensin CRC treatment when data was pooled over the 4 experiments in which bloat occurred. Daily milk yield was increased in all experiments from a mean of 17.7 in untreated groups to 18.8 kg/head/day (P less than 0.05) in monensin CRC-treated cows. Protein percentage was not affected by treatment, while there was a decrease from 4.29 to 4.10% fat, although total fat yield was not affected. (ABSTRACT TRUNCATED AT 250 WORDS)
Stochastic calculus and derivatives pricing in theNigerian stock market

Led by the Central Bank of Nigeria (CBN) and the Nigerian Stock Exchange (NSE), policy makers, investors and other stakeholders in the Nigerian Stock Market consider the introduction of derivative products in Nigerian capital markets essential for their investment and risk management needs. This research foregrounds these interests through detailed theoretical and empirical review of derivative pricing models. The specific objectives of the research include: 1) To explore the key stochastic calculus models used in pricing and trading financial derivatives (e.g. the Black-Scholes model and its extensions); 2) To examine the investment objectives fulfilled by derivatives; 3) To investigate the links between the stylized facts in the Nigerian Stock Market (NSM), the risk management techniques to be adopted, and the workings of the pricing models; and 4) To apply the research results to the NSM, by comparing the investment performance of selected derivative pricing models under different market scenarios, represented by the stylized facts of the underlying assets and market characteristics of the NSM. The foundational concepts that underpin the research include: stochastic calculus models of derivative pricing, especially the Black-Scholes (1973) model; its extensions; the practitioners’ Ad-Hoc Black Scholes models, which directly support proposed derivative products in the NSM; and Random Matric Theory (RMT). RMT correlates market data from the NSM and Johannesburg Stock Exchange (JSE) and facilitates possible simulation of non-existing derivative prices in the NSM, from those in the JSE. Furthermore, the research explores in detail the workings of different derivative pricing models, for example various structures for the Ad-Hoc Black Scholes models, using selected underlying asset prices, to determine the applicability of the models in the NSM. The key research findings include: 1) ways to estimate the parameters of the stochastic calculus models; 2) exploring the benefits of introducing pioneer derivative products in the NSM, including risk hedging, arbitrage, and price speculation; 3) using NSM stylized facts to calibrate selected derivative pricing models; and 4) explaining how the results could be used in future experimental modelling to compare the investment performance of selected models. By way of contributions to knowledge, this is the first study known to the researcher that provides in-depth review of the theoretical and empirical underpinnings of derivative pricing possible in the NSM. This forms the basis for the Black Scholes approach to asset pricing of European option contract, which is the kind of call/put option contract that is being adopted in the NSM. The research provides the initial foundations for effective derivatives trading in the NSM. By explaining the heuristics for developing derivative products in the NSM from JSE information, the research will support future work in this important area of study.
Analysis of the Newsboy Problem subject to price dependent demand and multiple discounts

Existing papers on the Newsboy Problem that deal with price dependent demand and multiple discounts often analyze those two problems separately. This paper considers a setting where price dependence and multiple discounts are observed simultaneously, as is the case of the apparel industry. Henceforth, we analyze the optimal order quantity, initial selling price and discount scheme in the News-Vendor Problem context. The term of discount scheme is often used to specify the number of discounts as well as the discount percentages. We present a solution procedure of the problem with general demand distributions and two types of price-dependent demand: additive case and multiplicative case. We provide interesting insights based on a numerical study. An approximation method is proposed which confirms our numerical results.
Can parasites use predators to spread between primary hosts?

SUMMARY Parasites typically have low reproductive fitness on paratenic hosts. Such hosts offer other significant inclusive fitness benefits to parasites, however, such as increased mobility and migration potential. The parasite fauna of the guppy (Poecilia reticulata) is dominated by the directly transmitted ectoparasites Gyrodactylus bullatarudis and Gyrodactylus turnbulli. In the wild, close predatory and competitive interactions occur between the guppy and the killifish Rivulus hartii. Previous observations suggest that these fish can share gyrodactylids, so we tested experimentally whether these parasites can use R. hartii as an alternative host. In aquaria, G. bullatarudis was the only species able to transmit from prey to predator. Both parasite species transferred equally well to prey when the predator was experimentally infected. However, in semi-natural conditions, G. bullatarudis transmitted more successfully to the prey fish. Importantly, G. bullatarudis also survived significantly longer on R. hartii out of water. As R. hartii can migrate overland between isolated guppy populations, G. bullatarudis may have an enhanced ability to disperse and colonize new host populations, consistent with its wider distribution in the wild. To our knowledge, this is the first empirical study demonstrating a predator acting as a paratenic host for the parasites of its prey.
Reclaiming Accountability in Teacher Education

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License Reclaiming Accountability in Teacher Education is the winner of both the 2019 Society of Professors of Education Outstanding Book Award and the 2018 AESA Critic’s Choice Award. After reading this book, it is obvious why Reclaiming Accountability in Teacher Education was honored with these prestigious awards. The book is an exemplary blend of history in the era of education reform, current accountability measures, and hopeful possibilities for reimagining accountability in teacher education.
Color stability of glass-ionomers and polyacid-modified resin-based composites in various environmental solutions.

PURPOSE To evaluate the degree of color stability of glass-ionomers (GIs) and polyacid modified resin-based composites (PMRBCs) in various environmental solutions. MATERIALS AND METHODS Seven polyacid-based esthetic restorative materials were used: three chemical-cured GIs, one resin-modified GI and three polyacid-modified RBCs. A light-cured resin-based composite (RBC) (Z 100) was used as a control. Disk type specimens were prepared and were aged in four different solutions (deionized water, 0.1 mole acetic acid solution, 75% ethanol, and 10% hydrogen peroxide solution) for 1, 7, 14, 21, 28, and 56 days. The specimens were kept at 37 degrees C throughout the study. Color coefficients (CIE L*a*b*) were measured by a reflection spectrophotometer with SCE mode, and the surface of specimens was examined by a stereo zoom microscope. RESULTS In deionized water, all specimens showed an acceptable color stability. All of the GIs and PMRBCs showed significant color change in 0.1 mole acetic acid solution. The light-cured resin-modified GI showed a significant color change in 75% ethanol solutions. 10% hydrogen peroxide solution resulted in degradation and a high degree of color change for chemical-cured GIs. The light-cured resin-modified GI and PMRBCs showed high color change in 10% hydrogen peroxide solution. The light-cured RBC (Control), showed excellent color stability in all experimental solutions.
The criteria of dissolved gas analysis in natural ester fluid

In this paper, dissolved gasses of natural ester fluid were analyzed to suggest DGA criteria for transformer filled with natural ester fluid. Samples of mineral oil and natural ester fluid made to correspond to the ratios of the iron core, winding, insulating materials and insulating oil in a transformer were deteriorated for 1,600 hours in a 150°C thermostat and samples were collected every two hundred hours to analyze changes in the contents of dissolved gases. As the results, H2 and C2H2 gases were hardly detected and C2H6 gas detected from natural ester fluid was two times larger in quantity than mineral oil. CH4 gas detected from natural ester fluid was 1/4 in quantity of the gas detected from mineral oil. Amount of other dissolved gasses were similar to each other. Based on these results, DGA criteria for transformers applied with natural ester fluid were presented.
Beginning SBAE Teachers' Metal Fabrication Knowledge Needs: Implications for Teacher Preparation.

While agricultural mechanics, which includes metal fabrication, continues to be popular among school-based agricultural education (SBAE) students (Hubert & Leising, 2000), the number of required courses in agricultural mechanics for teaching certification in agricultural education is relatively low (Blackburn, Robinson, & Field, 2015). Despite the minimal required coursework, Saucier and McKim (2010) argued that all SBAE teachers who instruct agricultural mechanics should be technically competent. To ensure preservice teachers are technically prepared to enter the profession, this study, guided by the model for teacher preparation (Whittington, 2005), sought to determine the essential metal fabrication knowledge and skills which beginning SBAE teachers should possess prior to beginning a career in agricultural education. Results from this study identified eight essential knowledge and skill areas categorized into four categories, metal fabrication equipment, metal fabrication production, student assessment, and laboratory management. Preservice teacher programs should be evaluated to determine if they are effectively preparing teachers in the curriculum area of metal fabrication. Teacher educators and professional development staff should plan professional education programs for in-service teachers in this agricultural mechanics area.
Tyl6, a novel Ty3/gypsy‐like retrotransposon in the genome of the dimorphic fungus Yarrowia lipolytica

The novel LTR retrotransposon Tyl6 was detected in the genome of the dimorphic fungus Yarrowia lipolytica. Sequence analysis revealed that this element is related to the well‐known Ty3 element of Saccharomyces cerevisiae and, especially, to the recently described Tse3 retrotransposon of Saccharomyces exiguus and to the del1‐like plant retrotransposons. Tyl6 is 5108 bp long, is flanked by two identical long terminal repeats (LTR), each of 276 bp, and its ORFs are separated by a −1 frameshift. Both ORFs are intact and deduced translation products display a significant similarity with those of previously described Ty3/gypsy retrotransposons. Distribution of Tyl6 among Y. lipolytica strains of different origins was also analysed. A single copy of the novel retrotransposon is present in some commonly used laboratory strains, which are derivatives of the wild‐type isolate YB423‐12, whereas other strains of independent origin are devoid of Ty16. No solo LTR of Tyl6 was detected in the analysed strains. The complete sequence of Tyl6 was submitted to the EMBL nucleotide sequence database under Accession No. AJ746250. Copyright © 2005 John Wiley & Sons, Ltd.