Miracle-dz/UniFAR-FTUs huggingface dataset:
EVOLUCIÓN GENÓMICA POR DISEÑO MOLECULAR DE LEVADURAS INDUSTRIALES

En esta Tesis Doctoral se propone una alternativa a la coyuntura actual de rechazo social frente al uso de OMGs en la industria agroalimentaria, mediante la demostracion y el desarrollo de un nuevo concepto sobre el uso de las tecnicas de biologia molecular en la obtencion de levaduras modificadas geneticamente, el concepto de Evolucion Genomica mediante Diseno Molecular. La idea basica de este nuevo concepto es simple y se basa en imitar a la propia naturaleza en su constante evolucion, pero acelerando y dirigiendo el proceso evolutivo con el objeto de seleccionar en un corto periodo de tiempo aquellos cambios geneticos que especificamente hayamos disenado a escala molecular. Las levaduras no son una entidad invariable en el tiempo, al contrario, como todos los organismos vivos evolucionan con el tiempo. Mediante esta nueva metodologia que hemos desarrollado, se consigue acelerar el proceso evolutivo, pues las modificaciones geneticas que hemos introducido se podrian haber efectuado espontaneamente por la levadura en su entorno natural como consecuencia de procesos de recombinacion, duplicacion o eliminacion de elementos geneticos a lo largo de su ciclo reproductivo, aunque indudablemente se habrian perdido sin la presencia de determinadas condiciones de seleccion y, dada la baja probabilidad de que esos eventos ocurrieran, habrian necesitado de un inmenso periodo de tiempo. Se ha demostrado el concepto de Evolucion Genomica mediante Diseno Molecular, mediante la construccion e integracion cromosomica de un casete de seleccion basado en la sobreexpresion del gen YAP1, que da origen a un marcador dominante que confiere resistencia a diversos compuestos toxicos para las levaduras, tales como cicloheximida y cerulenina. Para el desarrollo de dicho casete de seleccion nos hemos impuesto y respetado las siguientes condiciones: 1) Utilizacion de la capacidad de recombinacion homologa de las levaduras del genero Saccharomyces; 2) Utilizacion de material genetico procedente exclusivamente de la propia cepa de levadura a evolucionar; 3) El material genetico no ha de sufrir ningun paso intermedio de clonaje o expresion en otros sistemas biologicos. Una vez cumplidas todas estas condiciones, las cepas resultantes no deberian responder a la definicion oficial de organismos modificados geneticamente de la Union Europea: ? organismos en los que su material genetico ha sido alterado de una manera que no sucede de forma natural mediante los procesos de reproduccion sexual y/o recombinacion natural? Para alcanzar el objetivo fundamental del proyecto, hemos trabajado inicialmente con un sistema modelo mas sencillo, como es el caso de una cepa de una levadura de laboratorio, y a continuacion hemos abordado la misma aproximacion experimental pero trabajando con una levadura que se emplea en la produccion industrial de cerveza tipo ? lager? La metodologia experimental que se ha disenado ha consistido en obtener, solo mediante reacciones de PCR, una construccion …
Multiwavelength Dissipative-Soliton Generation in Yb-Fiber Laser Using Graphene-Deposited Fiber-Taper

We propose and demonstrate multiwavelength dissipative soliton (DS) generation in an all-normal-dispersion ytterbium-doped fiber laser based on a graphene-deposited tapered fiber (GDTF) device. Due to the interaction of the graphene with the evanescent field on the taper, the GDTF device possesses the characteristics of both saturable absorption and polarizing effect. Therefore, this device not only initiates the mode-locking operation based on the saturable absorption, but also induces both the special spectral filtering and nonlinear polarization evolution for shaping pulses into DSs. Simultaneous triple-wavelength DS operation around 1035 nm is thus achieved with a pulse energy of 6.4 nJ and pulse duration of 74.6 ps. Moreover, the DS operation is very stable with an RF signal-to-noise ratio of 62.5 dB.
Can Information Retrieval Techniques Effectively Support Traceability Link Recovery?

Applying information retrieval (IR) techniques to retrieve all correct links between software artefacts is in general impractical, as usually this means producing a high effort for discarding too many false positives. We show that the only way to recover traceability links using IR methods is to identify an "optimal" threshold that achieves an acceptable balance between traced links and false positives. Unfortunately, such threshold is not known a priori. For this reason we have devised the need to use an incremental traceability recovery approach to gradually identify the threshold where it is more convenient to stop the traceability recovery process, and provide evidence of this in a case study. We also report the experience of using the incremental traceability recovery during the development of software projects
Substrate-strained silicon technology: process integration [CMOS technology]

We demonstrate a 60 nm gate length substrate-strained Si CMOS technology and the fastest reported ring oscillator speed of 6.5 ps at 1.2 V operation. The largest enhancement (15%) in I/sub on/-I/sub off/ characteristics without correction for self-heating effects is also reported. The substrate-strained Si process is optimized to enhance manufacturability and circumvent difficulties associated with the integration of the strained Si/SiGe heterostructure. We also report a phenomenon responsible for increased the off state leakage in strained Si devices and a way to suppress it. Surmounting key integration challenges faced by the Si/SiGe heterostructure is critical for its introduction as a manufacturable process.
Germiston virus transcriptase requires active 40S ribosomal subunits and utilizes capped cellular RNAs

The transcriptase associated with Germiston virus was assayed in an in vitro reaction in which transcription was coupled to translation by adding reticulocyte lysate under the appropriate salt conditions. When analyzed in polyacrylamide gels, the major transcripts migrated like authentic S mRNAs and possessed 12- to 18-base-long nontemplated 5' extensions similar to the 5' end of viral mRNAs. These transcripts were functional for the synthesis of at least proteins N and NSS. When translation was inhibited by adding protein synthesis inhibitors such as puromycin, cycloheximide, and anisomycin, a drastic inhibitory effect was observed on the synthesis of the complete S mRNA transcripts. However, initiation and part of the elongation process were still active, since short and incomplete RNA molecules with RNA primers at their 5' ends were synthesized. On the other hand, we found that edeine, another inhibitor of protein synthesis, stimulated not only synthesis of S mRNAs but also that of the full-length S cRNAs. Taking into account the mode of action of this antibiotic, we discuss the results, which emphasize the crucial role of active ribosomes during bunyavirus transcription and confirm the observations reported on La Crosse virions. Moreover, we showed that the RNA transcripts synthesized in a transcription-translation reaction were capped and that most of them have acquired the 5' terminal sequences of the alpha- or beta-globin mRNA.
Performance evaluation of single ended primary inductance converter for photo voltaic applications

This paper aims at the discussion of the performance parameters evaluation of Non isolated Single Ended Primary Inductance DC-DC Converter over Buck Boost and Cuk type converter, whose primary role in photovoltaic power generation is to maintain the output voltage constant irrespective of changes in the input, with improved efficiency and less input current ripple. The paper includes dynamic modeling and simulation of a photovoltaic cell under varying insolation and changes in temperature along with the modeling of the three topologies mentioned above taking the unregulated voltage from the pv cell and their performance parameters are being compared to choose the better converter topology for solar power generation system. For modeling the pv cell the single diode equivalent diagram is considered simulated using MATLAB / Simulink platform to obtain PV and IV curves of solar cell with variable temperature and variable irradiance. Also the dc-dc converters are modeled in MATLAB, and the simulation results are compared.
Is multicomponent T2 a good measure of myelin content in peripheral nerve?

Multicomponent T2 relaxation of normal and injured rat sciatic nerve was measured. The T2 relaxation was multiexponential, indicating the multicompartmental nature of T2 decay in nerve tissue. The size of the short, observed T2 component correlated very well with quantitative assessment of myelin using computer‐assisted histopathological image analysis of myelin. Specifically, the size of the short T2 component reflected the processes of myelin loss and remyelination accompanying Wallerian degeneration and regeneration following trauma. However, it represented all myelin present in the sample and did not distinguish between intact myelin and myelin debris. Other changes in T2 spectra were also observed and could be correlated with axonal loss and inflammation. The study also questions the validity of previously offered interpretations of T2 spectra of nerve. Magn Reson Med 49:638–645, 2003. © 2003 Wiley‐Liss, Inc.
Identifying correlates of young adults' weight behavior: survey development.

OBJECTIVE To describe the development and psychometric properties of survey measures relevant to eating, physical activity, and weight-related behaviors among young adults. METHODS Focus groups and reliability testing guided the development of the Project EAT-III survey. The final survey was completed by 2287 young adults. RESULTS The systematic process employed led to a psychometrically sound and developmentally appropriate survey. Test-retest reliabilities for items included on the final survey were mostly moderate to good, and Cronbach alphas were >0.7 for 83% of developed scales. CONCLUSIONS Future studies may find the systematic process used to be helpful in creating other weight-related surveys.
Goodness‐of‐fit tests for high dimensional linear models

We propose a framework for constructing goodness‐of‐fit tests in both low and high dimensional linear models. We advocate applying regression methods to the scaled residuals following either an ordinary least squares or lasso fit to the data, and using some proxy for prediction error as the final test statistic. We call this family residual prediction tests. We show that simulation can be used to obtain the critical values for such tests in the low dimensional setting and demonstrate using both theoretical results and extensive numerical studies that some form of the parametric bootstrap can do the same when the high dimensional linear model is under consideration. We show that residual prediction tests can be used to test for significance of groups or individual variables as special cases, and here they compare favourably with state of the art methods, but we also argue that they can be designed to test for as diverse model misspecifications as heteroscedasticity and non‐linearity.
Quantitation of human herpesvirus 6 DNA in infant with exanthem subitum by microplate PCR‐hybridization assay

Abstract Background : Quantitative analysis of human herpesvirus 6 (HHV‐6) genome is important for monitoring active virus infection. The purpose of our study is to evaluate the reliability of a hybridization‐based microtiter plate assay (polymerase chain reaction enzyme‐linked immunosorbent assay (PCR ELISA)) for quantifying the virus genome.
Three-part passive constitutive laws for the aorta in simple elongation

A variety of uniaxial constitutive laws have been proposed for the characterization of the aorta's nonlinear passive response, but a detailed comparison of them appears to be lacking. In this study, a systematic presentation of all available phenomenological formulations is undertaken and explicit formulae of constitutive laws are provided for simple elongation tests performed on healthy aortic strips. Common to all derived laws is the use of three analytical functions to approximate the low, physiologic, and high-stress parts of the aortic response, and the very close and essentially equally accurate fits that they give to the experimental data over the full range of stresses. Another feature of the three-part laws is their compatibility with the biphasic nature of the aortic tissue under passive conditions, allowing direct microstructural interpretation of their parameters. Importantly, although it is found that the aorta displays strain softening, i.e. its passive response is dependent on the highest previously experienced stress, the three-part character of the laws seems to be unaffected by the preconditioning procedure.
Appearance (Ethnic Origin) of Requester Affects the Foot-In-The-Door Technique

A study was designed to examine the effect of the appearance of the requester within one of the variants of the foot-in-the-door paradigm, that is, the foot-in-the-door with implicit demand described by Uranowitz in 1975. A confederate (Black vs Blanc vs Beur2) approached the participant in a park and presented a small request. Three steps further, the confederate “accidentally” dropped 30 sheets of paper. Whether or not the participant helped the confederate in retrieving the dropped pamphlets was recorded as the implicit dependent variable. The foot-in-the-door effect was observed solely when the requester was Blanc. This result shows that the foot-in-the-door effect is not as strong as the literature suggests and undermines the usual interpretations of the foot-in-the-door effect in terms of self-perception and commitment.
Patterns of Spontaneous Purkinje Cell Complex Spike Activity in the Awake Rat

The olivocerebellar system is known to generate periodic synchronous discharges that result in synchronous (to within 1 msec) climbing fiber activation of Purkinje cells (complex spikes) organized in parasagittally oriented strips. These results have been obtained primarily in anesthetized animals, and so the question remains whether the olivocerebellar system generates such patterns in the awake animal. To this end, multiple electrode recordings of crus 2a complex spike activity were obtained in awake rats conditioned to execute tongue movements in response to a tone. After removal of all movement- and tone-related activity, the remaining data were examined to characterize spontaneous complex spike activity in the alert animal. Spontaneous complex spikes occurred at an average firing rate of 1 Hz and a clear ≈10 Hz rhythmicity. Analysis of the autocorrelograms using a rhythm index indicated that the large majority of Purkinje cells displayed rhythmicity, similar to that in the anesthetized preparation. In addition, the patterns of synchronous complex spike activity were also similar to those observed in the anesthetized preparation (i.e., simultaneous activity was found predominantly among Purkinje cells located within the same parasagittally oriented strip of cortex). The results provide unequivocal evidence that the olivocerebellar system is capable of generating periodic patterns of synchronous activity in the awake animal. These findings support the extrapolation of previous results obtained in the anesthetized preparation to the waking state and are consistent with the timing hypothesis concerning the role of the olivocerebellar system in motor coordination.
Big data y Psicología: ¿una oportunidad para el Internet de las personas?

En este trabajo se reflexiona sobre el concepto big data y su relacion con la Psicologia en ambitos como la evaluacion, la intervencion, la investigacion y la docencia. El concepto big data hace referencia literal al uso de grandes bases de datos, pero tambien a las tecnologias y metodologias que permiten recogerlas a traves de dispositivos de diversos tipos, entre los que destaca el telefono inteligente o smartphone. La Psicologia no puede ser ajena al desarrollo de esta tecnologia, y debe trabajar a varios niveles para hacer posible que los profesionales accedan lo antes posible a estas herramientas, y se pase de la mera fascinacion a la concrecion de estudios y a la investigacion para que contribuyan al avance de la disciplina.
U.S. Beef Demand Drivers and Enhancement Opportunities: A Research Summary

Large shifts in domestic beef demand have had substantial impacts on the beef industry. Before the late 1970s, growth in the U.S. economy and rising consumer incomes contributed to beef demand increasing for a sustained period. In response to growing product demand, the beef industry increased in size. However, starting about 1980, domestic retail beef demand weak-ened and subsequently declined every year through 1998. The long-run decline in retail beef demand contributed to a reduction in cattle industry size, particularly in rela-tion to competing meat sectors such as poultry and pork. In 1999, following nearly 20 consecutive years of decline, domestic beef demand began to strengthen. From the late 1990s through 2004, the all fresh domestic retail beef demand index increased from a low of 76 to a peak of 92, before weakening again from 2005 through 2008.Designing programs to increase domestic retail beef demand requires a comprehensive understanding of the many underlying factors that caused beef demand to decline precipitously during the 1980s and 1990s. Likewise, it is important to determine what caused recent beef demand increases from the late 1990s through the middle part of this decade. Increasing consumer demand for beef requires concerted effort by all vertical segments of the production, processing, and marketing chain as there are myriad opportunities to improve product quality, food safety, and diversity of product offerings. How the industry collectively responds to these challenges will ultimately determine the success or failure of demand enhancement initiatives. The purpose of this study was to provide a compre-hensive and updated assessment of factors influencing U.S. consumer demand for beef. To assess the relative impact various factors have on beef demand, a demand model was built to estimate the impacts of beef, competing meat, and other goods prices; consumer expenditures; published information on food safety, health and nutrition information related to meat consumption; female labor force participation; and expenditures on food consumed away from home. Quarterly data from 1982 through 2007 were used to estimate the model. Estimates obtained from the model provide measures of expected impacts from changes in each of the demand drivers. This fact sheet focuses on the impact of key demand drivers on U.S. consumer demand for beef and what these results mean for the U.S. beef industry.
Human chorioretinal layer thicknesses measured in macula-wide, high-resolution histologic sections.

PURPOSE To provide a comprehensive description of chorioretinal layer thicknesses in the normal human macula, including two-layer pairs that can produce a combined signal in some optical coherence tomography (OCT) devices (ganglion cell [GCL] and inner plexiform [IPL] layers and outer plexiform [OPL] and outer nuclear [ONL] layers). METHODS In 0.8-μm-thick, macula-wide sections through the foveola of 18 donors (age range, 40-92 years), 21 layers were measured at 25 locations by a trained observer and validated by a second observer. Tissue volume changes were assessed by comparing total retinal thickness in ex vivo OCT and in sections. RESULTS Median tissue shrinkage was 14.5% overall and 29% in the fovea. Histologic laminar boundaries resembled those in SD-OCT scans, but the shapes of the foveolar OPL and ONL differed. Histologic GCL, IPL, and OPLHenle were thickest at 0.8. to 1, 1.5, and 0.4 mm eccentricity, respectively. ONL was thickest in an inward bulge at the foveal center. At 1 mm eccentricity, GCL, INL, and OPLHenle represented 17.3% to 21.1%, 18.0% to 18.5%, and 14.2% to 16.6% of total retinal thickness, respectively. In donors ≥ 70 years of age, the RPE and choroid were 17.1% and 29.6% thinner and OPLHenle was 20.8% thicker than in donors <70 years. CONCLUSIONS In this study, the first graphic representation and thickness database of chorioretinal layers in normal macula were generated. Newer OCT systems can separate GCL from IPL and OPLHenle from ONL, with good agreement for the proportion of retinal thickness occupied by OPLHenle in OCT and histology. The thickening of OPLHenle in older eyes may reflect Müller cell hypertrophy associated with rod loss.
Unusual Cardiac Metastasis of Uterine Leiomyosarcoma: Case Report and Literature Review

Background Uterine leiomyosarcoma (LMS) is a rare malignancy of mesenchymal tissues and in advanced stages its prognosis is very poor. Surgery followed by radiotherapy and/or chemotherapy is the treatment of choice for advanced disease. Cardiac metastases are very uncommon and only a few cases have been described to date. Case A 55-year-old woman was referred to our center for a uterine LMS with lung metastases at diagnosis. After 3 lines of chemotherapy for persistent lung disease, CT scan showed suspected thrombosis in the right pulmonary vein, along with disease progression in the lungs. The patient started treatment with low-molecular-weight heparin and a fourth line of chemotherapy. After 3 months of therapy, a new CT scan showed a larger thrombus and she underwent a cardiology visit that revealed an intracardiac mass. Submitting the patient to palliative surgery or radiation therapy was not possible because of the aggressiveness of the lung metastases, so she continued chemotherapy, resulting in disease stabilization. Conclusions Surgery is the best option for intracardiac dissemination of uterine LMS, but when this is not possible based on the performance status of the patient and spread of the disease, the combination of chemotherapy and radiotherapy seems to be the best option according to the literature. In our case we treated the patient only with chemotherapy.