Miracle-dz/UniFAR-FTUs huggingface dataset:
THREE ESSAYS ON FOREIGN AID AND ETHNIC LEADERSHIP IN AFRICA

One major reason for the ineffectiveness of foreign aid in promoting development is recipient leaders and governments’ deviation from donors’ aid plans. In this research, I provide a theoretical explanation of leaders’ deviation from aid agreements in Africa. I argue that only by staying in power can leaders pursue other political goals. African societies share the characteristic that the national leaders stay in power by providing private goods to a small group of key supporters. These key supporters are commonly found in Africa to be those who share the leaders’ ethnicity. A leader with imminent threat to political survival would allocate all resources available to key supporters to consolidate political support, which would be reflected by higher level of ethnic favoritism in aid allocation in African countries. On the contrary, a leader with low risk of losing power could be motivated to invest in the long-term economic development of the country. With three empirical essays, I provide evidences consistent with this explanation. Essay One shows that subnational aid allocation commonly favor regions where a majority of the population share the leaders’ ethnicity. I find in Essay Two that such ethnic favoritism in aid allocation is more significant when a leader has higher risk of losing political power. As leaders increase allocation of aid to co-ethnic population for political survival, people from disadvantaged ethnic groups experience higher level of infant mortalities. Essay Three shows that high level of aid volatility threatens recipient leaders’ political survival and makes aid allocation more inefficient. My essays support my theoretical explanation that leaders are rationally motivated to influence subnational aid allocations. While under certain domestic constraints they may strategically choose to deviate from aid plans, in other scenarios they may actually share donors’ goals for long-term development. In conclusion, I call for donors to stabilize aid flow to increase aid effectiveness. I also suggest extra care to be taken by donors when determining whether some deviations from aid plans should be penalized.
Contribution of Glycosaminoglycans to the Microstructural Integrity of Fibrillar and Fiber Crimps in Tendons and Ligaments

The biomechanical roles of both tendons and ligaments are fulfilled by the extracellular matrix of these tissues. In particular, tension is mainly transmitted and resisted by protein (collagen, elastin) fibers, whereas compression is opposed by water-soluble glycosaminoglycans (GAGs). GAGs spanning the interfibrillar spaces and interacting with fibrils through the interfibrillar proteoglycans also seem to play a part in transmitting and resisting tensile stresses. Both tendons and ligaments showing similar composition, but different functional roles and collagen array, exhibit periodic undulations of collagen fibers or crimps. Each crimp is composed of many knots of each single fibril or fibrillar crimps. Fibrillar and fiber crimps play a mechanical role in absorbing the initial loading during elongation of both tendons and ligaments, and in recoiling fibrils and fibers when tissues have to return to their original length. This study investigated whether GAGs covalently attached to proteoglycan core proteins directly affect the 3D microstructural integrity of fibrillar crimp regions and fiber crimps in both tendons and ligaments. Achilles tendons and medial collateral ligaments of the knee from eight female Sprague-Dawley rats (90 days old) incubated in a chondroitinase ABC solution to remove GAGs were observed under a scanning electron microscope (SEM). In addition, isolated fibrils of these tissues obtained by mechanical disruption were analyzed by a transmission electron microscope (TEM). Both Achilles tendons and medial collateral ligaments of the rats after chemical or mechanical removal of GAGs still showed crimps and fibrillar crimps comparable to tissues with a normal GAG content. All fibrils in the fibrillar crimp region always twisted leftwards, thus changing their running plane, and then sharply bent, changing their course on a new plane. These data suggest that GAGs do not affect structural integrity or fibrillar crimp functions that seem mainly related to the local fibril leftward twisting and the alternating handedness of collagen from a molecular to a supramolecular level.
Antigen-specific immunomodulation of collagen-induced arthritis with tumor necrosis factor-stimulated dendritic cells.

OBJECTIVE Dendritic cells (DCs) are crucial for the initiation of T cell immunity and therefore play an important role in the initiation and regulation of immune responses in arthritis. Full mobilization of effector T cells depends on the proper maturation of DCs. Current evidence indicates that the type of T cell response induced is crucially dependent on the activation status of the DCs. In this study, we explored the immunologic effects of differentially matured DCs on the development of collagen-induced arthritis (CIA). METHODS Bone marrow-derived DCs were cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). Before immunization with bovine type II collagen (CII) protein, mice were repeatedly injected with DCs that had been pulsed with CII. Immature, semimature, or fully mature DCs were injected. Mice were boosted on day 21 after CII immunization, and the disease course was monitored. RESULTS While vaccination with immature or lipopolysaccharide-activated DCs had no significant effect on the disease course, administration of antigen-loaded, tumor necrosis factor (TNF)-modulated DCs propagated in GM-CSF with or without interleukin-4 resulted in a delayed onset of arthritis and a lower clinical score. The response was antigen-specific, since TNF-treated DCs pulsed with a control antigen did not modify the disease course. A specific decrease in the collagen-specific "Th1-associated" IgG2a response was observed, whereas IgG1 titers were unaffected. CONCLUSION CIA can be prevented through vaccination with TNF-matured DCs in an antigen-specific manner. These findings provide a rationale for immunotherapy using DCs in rheumatoid arthritis.
Effects of the oviduct and wheat germ agglutinin on enzymatic digestion of porcine zona pellucidae.

Seventy-two crossbred gilts were utilized to examine whether the oviduct rendered zona pellucidae resistant to protease digestion, whether the uterus reversed this resistance and whether such a uterine reversal was necessary for hatching. Oocytes were aspirated from follicles 22 to 28 h after onset of estrus (d 0); oviductal and uterine oocytes or embryos were collected on d 1 to 6. These oocytes and embryos were subjected to a solution containing . 1% trypsin and . 1% pronase (37 degrees C) for observation of zona pellucidae digestion. Zonae of oviductal oocytes were more (P less than .001) resistant to digestion than were follicular oocytes. Placement of follicular oocytes in oviducts for 30 min rendered zona pellucidae more (P less than .001) resistant to protease digestion than oocytes not exposed to oviductal secretions. Resistance of zona pellucidae to proteases, however, decreased (P less than .001) after entry into the uterus. Zonae of morulae retained in oviducts took longer (P less than .001) to digest than those recovered from the uterus. Blastocysts also were treated with wheat germ agglutinin (WGA; 50 micrograms/ml) for 40 min to determine whether artificial induction of zona resistance to enzymatic digestion affected the ability of embryos to hatch. Though WGA treatment delayed (P less than .001) enzymatic digestion of zona pellucidae, time from collection to hatching was not affected. This experiment indicated that the oviduct delayed enzymatic digestion of the zona pellucidae, whereas the uterus reversed this delay. The re-establishment of enzyme susceptibility after uterine entry, however, appeared to be unrelated to the subsequent ability of blastocysts to hatch.
Myristylation of pp60c-src is not required for complex formation with polyomavirus middle-T antigen

Middle-T antigen (middle-T), the transforming gene product of polyomavirus, associates with several cellular tyrosine kinases, such as pp60c-src. Complex formation leads to kinase activation and is essential for cell transformation. Middle-T-associated as well as uncomplexed pp60c-src is predominantly found in the plasma membrane. We transfected mouse 3T3 fibroblasts with a mutated c-src gene (2Ac-src), allowing the expression of a protein containing alanine instead of glycine in position 2 of the primary translation product. Contrary to the wild-type c-src gene product, pp60c-src(2A) was not myristylated and accumulated in the cytoplasm instead of being transferred to cellular membranes. The mutant protein was able to associate with middle-T and was activated similarly to the wild-type c-src gene product. Both wild-type and 2A mutant protein were membrane associated upon complex formation with middle-T. This finding suggests that the putative carboxy-terminal membrane anchor sequence of middle-T is sufficient to hold middle-T-associated pp60c-src(2A) in the plasma membrane.
Green space is associated with walking and moderate-to-vigorous physical activity (MVPA) in middle-to-older-aged adults: findings from 203 883 Australians in the 45 and Up Study

Background Green space is widely hypothesised to promote physical activity. Few studies, however, examine whether this is the case for walking and moderate-to-vigorous physical activity (MVPA). We investigated to what extent neighbourhood green space was associated with weekly participation and frequency of walking and MVPA in a large cross-sectional survey of Australian adults 45 years and older. Methods Logit and negative binomial regression were used to estimate the degree of association between walking, MVPA and neighbourhood green space in a sample of 203 883 adults from the Australian 45 and Up Study. Walking and MVPA were measured using the Active Australia Survey. Green space was measured as a percentage of the total land-use within 1 km radius of residence. We controlled for a range of individual and neighbourhood characteristics. Results 86.6% of the sample walked and 85.8% participated in MVPA at least once a week. These rates fell steeply with age. Compared with residents of neighbourhoods containing 0–20% green space, those in greener areas were significantly more likely to walk and participate in MVPAs at least once a week (trend for both p<0.001). Among those participating at least once a week, residents of neighbourhoods containing 80%+ green space participated with a greater frequency of walking (incidence rate ratio (IRR) 1.09, 95% CI 1.05 to 1.13) and MVPA (IRR 1.10, 95% CI 1.05 to 1.15). Conclusions Our findings suggest that the amount of green space available to adults in middle-to-older age within their neighbourhood environments could help to promote walking and MVPA.
Transmission of force sensation by environment quarrier based on multilateral control

In recent years, realization of a haptic system is desired strongly in the fields of medical treatment and expert's skill acquisition. The bandwidth of force sensing and friction compensation are very important problems for reproduction of vivid force sensation. In this paper, an environment quarrier is proposed for bilateral teleoperation instead of force sensors. The environment quarrier is a novel force sensing method which consists of a twin robot system. Two same types of robots are required and they are controlled in the same position, velocity and acceleration by bilateral acceleration control based on a disturbance observer. One robot is in contact motion and the other is unconstrained. The purity of external force is obtained by subtracting the disturbance torque in the unconstrained robot from the constrained one. The environment quarrier can observe the external force with high bandwidth and friction compensation. In order to implement the environment quarrier to a bilateral control system, four robots are required. In this paper, the idea of multilateral control is introduced for attainment of simultaneity. Furthermore, this paper shows that the controller design of the multilateral control in the modal space. The experimental results showed the viability of the proposed method.
What is the Best Route to the Meckel Cave? Anatomical Comparison between the Endoscopic Endonasal Approach and a Lateral Approach

Abstract Background Traditionally, a pterional approach is utilized to access the Meckel cave. Depending on the tumor location, extradural dissection of the Gasserian ganglion can be performed. An endoscopic endonasal access could potentially avoid a craniotomy in these cases. Methods We performed an endoscopic endonasal approach as well as a lateral approach to the Meckel cave on six anatomic specimens. To access the Meckel cave endoscopically, a complete sphenoethmoidectomy and maxillary antrostomy followed by a transpterygoid approach was performed. For lateral access, a pterional craniotomy with extradural dissection was performed. Results The endoscopic endonasal approach allowed adequate access to the Gasserian ganglion. All the relevant anatomy was identified without difficulty. Both approaches allowed for a similar exposure, but the endonasal approach avoided brain retraction and improved anteromedial exposure of the Gasserian ganglion. The lateral approach provided improved access posterolaterally and to the superior portion. Conclusion The endoscopic endonasal approach to the Meckel cave is anatomically feasible. The morbidity associated with brain retraction from the open approaches can be avoided. Further understanding of the endoscopic anatomy within this region can facilitate continued advancement in endoscopic endonasal surgery and improvement in the safety and efficacy of these procedures.
Recent developments in PET detector technology

Positron emission tomography (PET) is a tool for metabolic imaging that has been utilized since the earliest days of nuclear medicine. A key component of such imaging systems is the detector modules—an area of research and development with a long, rich history. Development of detectors for PET has often seen the migration of technologies, originally developed for high energy physics experiments, into prototype PET detectors. Of the many areas explored, some detector designs go on to be incorporated into prototype scanner systems and a few of these may go on to be seen in commercial scanners. There has been a steady, often very diverse development of prototype detectors, and the pace has accelerated with the increased use of PET in clinical studies (currently driven by PET/CT scanners) and the rapid proliferation of pre-clinical PET scanners for academic and commercial research applications. Most of these efforts are focused on scintillator-based detectors, although various alternatives continue to be considered. For example, wire chambers have been investigated many times over the years and more recently various solid-state devices have appeared in PET detector designs for very high spatial resolution applications. But even with scintillators, there have been a wide variety of designs and solutions investigated as developers search for solutions that offer very high spatial resolution, fast timing, high sensitivity and are yet cost effective. In this review, we will explore some of the recent developments in the quest for better PET detector technology.
Pathology of experimental inhalation anthrax in the rhesus monkey.

BACKGROUND The inhalation form of anthrax, although rare, is nearly always fatal because of the rapid progression of the disease with little host response until the terminal stages of the disease. The Gulf War heightened the concern that anthrax could be used as a biologic weapon. Past studies modeling pathologic changes in human inhalation anthrax have used the rhesus monkey. EXPERIMENTAL DESIGN We studied pathologic changes in the rhesus monkey model of inhalation anthrax. Gross examination as well as light and electron microscopy were used to define pathologic alterations. Immunolabeling techniques were used to identify the anthrax bacillus by light and electron microscopy. RESULTS Gross changes included hemorrhage in mesenteric (54%) and tracheobronchial (46%) lymph nodes, meninges (38%), lungs (31%), and small intestinal serosa (31%). Histopathologic changes included suppurative meningitis (77%); hemorrhages in the meninges (54%), neuropil (31%), and pulmonary alveoli (31%); and pneumonia (15%). Spleens and various lymph nodes from all monkeys had one or more of the following changes: hemorrhage, acute inflammation, extracellular bacilli, lymphocytic depletion, and histiocytosis. Spleens of two monkeys were devoid of extracellular bacilli, but degraded intrahistiocytic bacilli reacted with Ab to Bacillus anthracis cell wall polysaccharide. CONCLUSIONS In our study, compared with previous reports, meningitis and mesenteric lymph node hemorrhages were more common, whereas mediastinal and tracheobronchial lymph node hemorrhages were less common. Immunostaining highlighted intracellular bacilli that would have been otherwise missed by light microscopic examination.
Background severity of asthma in children discharged from the emergency department

Objective: Attendance at an Emergency Department (ED) with an acute attack of asthma may be indicative of undertreatment of persistent disease. However, many presentations are in children with infrequent episodic asthma. The aim of this study was to characterize the pattern of asthma of children discharged from ED to determine whether there was potential to improve underlying disease control.
The power of ideas : the state-sector relationship in policy and practice.

This thesis explores the relationship between the state and the voluntary sector as expressed within national documents and individual actor accounts. It is a response to the tendency within existing studies to cast voluntary sector organisations as passive in their context, responding to ideas from the central state rather than playing an active role in how and why particular ideas take hold. By first considering how the voluntary sector emerged as an object of policy, this thesis moves on to explore how both national texts and individual actors within organisations discursively present the role of the voluntary sector, the role of the state, and the relationship between the two. In order to explore this topic, this study uses a discursive methodology to consider the ideas within national documents and narratives of actors from Local Infrastructure Organisations (LIOs). This study drew on discursive approaches, such as discourse analysis, and sought to consider how and why ideas were constructed and used within different texts. This thesis contributes to knowledge in a number of ways. First, within the study of national policy documents, this thesis proposes an approach to bridging empirical studies of the societal and the micro levels of analysis, drawing on concepts of institutional logics and institutional message to demonstrate how national documents construct and convey institutional identity-cues for voluntary sector organisations. Second, within the study of the narrative of LIO actors, this study makes a series of contributions relating to the way we conceptualise what is happening at the organisational field level, linking the shifts in ideas at the societal level with the actor accounts of the implications of, and responses, to these shifting institutional logics. Third, the discursive analysis of actor accounts enables a consideration of the ways in which LIO actors are engaging in forms of identity work in order to align with, and distance from, voluntary sector identity-cues presented within national documents. These contributions are significant, particularly in light of debates surrounding the deepening austerity agenda, and associated cuts to services across communities in England. Such cuts have, at times, been presented within a narrative of the voluntary sector emerging to 'fill the gaps' and it is therefore important to start to understand how voluntary sector actors might be responding to such cues. This study therefore has implications for both sectors but also beneficiaries of both state and voluntary sector services, as it illuminates the way in which ideas are developing, and the way in which these ideas are reworked by actors at the local level.
Tombusvirus Y-Shaped Translational Enhancer Forms a Complex with eIF4F and Can Be Functionally Replaced by Heterologous Translational Enhancers

ABSTRACT Certain plus-strand RNA plant viruses that are uncapped and nonpolyadenylated rely on RNA elements in their 3′ untranslated region, termed 3′-cap-independent translational enhancers (3′CITEs), for efficient translation of their proteins. Here, we have investigated the properties of the Y-shaped class of 3′CITE present in the tombusvirus Carnation Italian ringspot virus (CIRV). While some types of 3′CITE have been found to function through recruitment of translation initiation factors to the viral genome, no trans-acting translation-related factors have yet been identified for the Y-shaped 3′CITE. Our results indicate that the CIRV 3′CITE complexes with eIF4F and eIFiso4F, with the former mediating translation more efficiently than the latter. In nature, some classes of 3′CITE are present in several different viral genera, suggesting that these elements hold a high degree of modularity. Here, we test this concept by engineering chimeric viruses containing heterologous 3′CITEs and show that the Y-shaped class of 3′CITE in CIRV can be replaced by two alternative types of 3′CITE, i.e., a Panicum mosaic virus-like 3′CITE or an I-shaped 3′CITE, without any major loss in in vitro translation or replication efficiency in protoplasts. The heterologous 3′CITEs also mediated whole-plant infections of Nicotiana benthamiana, where distinct symptoms were observed for each of the alternative 3′CITEs and 3′CITE evolution occurred during serial passaging. Our results supply new information on Y-shaped 3′CITE function and provide insights into 3′CITE virus-host compatibilities.
HIF1α is a central regulator of collagen hydroxylation and secretion under hypoxia during bone development

Collagen production is fundamental for the ontogeny and the phylogeny of all multicellular organisms. It depends on hydroxylation of proline residues, a reaction that uses molecular oxygen as a substrate. This dependency is expected to limit collagen production to oxygenated cells. However, during embryogenesis, cells in different tissues that develop under low oxygen levels must produce this essential protein. In this study, using the growth plate of developing bones as a model system, we identify the transcription factor hypoxia-inducible factor 1 α (HIF1α) as a central component in a mechanism that underlies collagen hydroxylation and secretion by hypoxic cells. We show that Hif1a loss of function in growth plate chondrocytes arrests the secretion of extracellular matrix proteins, including collagen type II. Reduced collagen hydroxylation and endoplasmic reticulum stress induction in Hif1a-depleted cells suggests that HIF1α regulates collagen secretion by mediating its hydroxylation and consequently its folding. We demonstrate in vivo the ability of Hif1α to drive the transcription of collagen prolyl 4-hydroxylase, which catalyzes collagen hydroxylation. We also show that, concurrently, HIF1α maintains cellular levels of oxygen, most likely by controlling the expression of pyruvate dehydrogenase kinase 1, an inhibitor of the tricarboxylic acid cycle. Through this two-armed mechanism, HIF1α acts as a central regulator of collagen production that allows chondrocytes to maintain their function as professional secretory cells in the hypoxic growth plate. As hypoxic conditions occur also during pathological conditions such as cancer, our findings may promote the understanding not only of embryogenesis, but also of pathological processes.