Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of neurobehavioral abnormalities and immunotoxicity in response to oral imidacloprid exposure in domestic chickens (Gallus gallus domesticus)

ABSTRACT Domestic chickens (Gallus gallus domesticus) were exposed to imidacloprid by gavage once daily for 7 consecutive days at 0, 0.03, 0.34, 3.42, 10.25, and 15.5 mg/kg/day (n = 20 per group; 5 6-week-old males, 5 6-week-old females, 5 9-week-old males, and 5 9-week-old females). The severity and duration of neurobehavioral abnormalities were recorded. Components of the innate and adaptive immune system were assessed with 7 standard functional assays. Temporary neurobehavioral abnormalities were observed in a dose-dependent manner, including muscle tremors, ataxia, and depressed mentation. Based upon mean clinical severity scores, the no observed adverse effect level (NOAEL) was 3.42 mg/kg/day, and the lowest observed adverse effect level (LOAEL) was 10.25 mg/kg/day. The effective dose value for the presence of any neurobehavioral abnormalities in 50% of the test group (ED50) was 4.62 ± 0.98 mg/kg/day. The ED50 for an adjusted score that included both severity and duration of neurobehavioral abnormalities was 11.24 ± 9.33 mg/kg/day. These ED50 values are equivalent to a 1 kg bird ingesting 29 or 70 imidacloprid treated soybean seeds respectively. Immunotoxicity was not documented, possible causes include the assays were insensitive, relevant immune functions were not examined, or imidacloprid is not immunotoxic at this dosing schedule in this species. Neurobehavioral abnormalities were a more sensitive indicator of the sublethal effects of imidacloprid than immunotoxicity.
Increasing Susceptibility of Drug-Resistant Candida albicans to Fluconazole and Terbinafine by 2(5H)-Furanone Derivative

The frequency of mycoses caused by drug-resistant fungal pathogen Candida albicans has increased drastically over the last two decades. The spread of drug-resistant strains, along with the limitations of currently available antifungals, complicates the management of fungal infections, thereby representing great challenges for clinical healthcare. Among various antimicrobial pharmacophores, 2(5H)-furanone derivatives have demonstrated antimicrobial, antifungal, and antibiofilm activities. In this study, we report the antifungal activity of the 2(5H)-furanone derivative F105, consisting of three pharmacophores, namely chlorinated 2(5H)-furanone, sulfonyl group, and l-menthol moiety. Although exhibiting moderate antifungal activity alone with the minimum inhibitory concentration (MIC) values of 32–256 μg/mL, F105 potentiates the activity of fluconazole and terbinafine with fractional inhibitory concentration index (FICI) values of 0.27–0.50. Thus, 16 μg/mL of F105 reduced the MICs of these antifungals against fluconazole-resistant C. albicans isolates four-fold, achieving similar values as for the intermediately susceptible phenotype. Confocal laser scanning microscopy revealed that the fluorescent 2(5H)-furanone derivative F145 was also able to penetrate through biofilms formed by C. albicans. Indeed, in the presence of F105, even sub-MIC concentrations of both fluconazole and terbinafine led to significant reduction of C. albicans CFUs in the mature biofilm. Thus, F105 appears to be a promising candidate for the development of novel antifungal agents as well as enhancers of current antifungal agents, particularly for the treatment of drug-resistant C. albicans infections.
A Farewell to Art: Aesthetics as a Topic in Psychology and Neuroscience

Empirical aesthetics and neuroaesthetics study two main issues: the valuation of sensory objects and art experience. These two issues are often treated as if they were intrinsically interrelated: Research on art experience focuses on how art elicits aesthetic pleasure, and research on valuation focuses on special categories of objects or emotional processes that determine the aesthetic experience. This entanglement hampers progress in empirical aesthetics and neuroaesthetics and limits their relevance to other domains of psychology and neuroscience. Substantial progress in these fields is possible only if research on aesthetics is disentangled from research on art. We define aesthetics as the study of how and why sensory stimuli acquire hedonic value. Under this definition, aesthetics becomes a fundamental topic for psychology and neuroscience because it links hedonics (the study of what hedonic valuation is in itself) and neuroeconomics (the study of how hedonic values are integrated into decision making and behavioral control). We also propose that this definition of aesthetics leads to concrete empirical questions, such as how perceptual information comes to engage value signals in the reward circuit or why different psychological and neurobiological factors elicit different appreciation events for identical sensory objects.
Incidence of Bacterial Chondronecrosis with Osteomyelitis (Femoral Head Necrosis) Induced by a Model of Skeletal Stress and its Correlation with Subclinical Necrotic Enteritis

Bacterial chondronecrosis with osteomyelitis (BCO) is a septic necrosis of the skeletal system of unknown origin and an important cause of lameness in broiler chickens. Epithelial inflammation has been proposed as an avenue for bacterial translocation leading to BCO. We evaluated the effect of subclinical necrotic enteritis (SNE), an intestinal inflammatory event, with the development of BCO. In each of two experiments, chickens were divided into three treatments: (1) SNE challenge, including both dietary (wheat- and fish-based diet) and intestinal pathogenic challenges (Eimeria maxima and Clostridium perfringens), (2) dietary challenge only, and (3) control diet (corn-and soy-based diet). Floor ramps were introduced as part of an established method for increasing the frequency of BCO. The efficacy of the SNE challenge was corroborated by necropsy evaluation of a representative sample of the population. At the end of each experiment, all birds were evaluated for BCO. A high incidence of BCO was found, even in birds with no external signs of lameness. However, the incidence of BCO was not correlated with the intestinal challenge. Conclusions: under the conditions used in these studies, a treatment that is associated with severe damage to the intestinal mucosa does not change the incidence of BCO in broiler chickens.
Detection and Mitigation of DoS and DDoS Attacks in IoT-Based Stateful SDN: An Experimental Approach

The expected advent of the Internet of Things (IoT) has triggered a large demand of embedded devices, which envisions the autonomous interaction of sensors and actuators while offering all sort of smart services. However, these IoT devices are limited in computation, storage, and network capacity, which makes them easy to hack and compromise. To achieve secure development of IoT, it is necessary to engineer scalable security solutions optimized for the IoT ecosystem. To this end, Software Defined Networking (SDN) is a promising paradigm that serves as a pillar in the fifth generation of mobile systems (5G) that could help to detect and mitigate Denial of Service (DoS) and Distributed DoS (DDoS) threats. In this work, we propose to experimentally evaluate an entropy-based solution to detect and mitigate DoS and DDoS attacks in IoT scenarios using a stateful SDN data plane. The obtained results demonstrate for the first time the effectiveness of this technique targeting real IoT data traffic.
Dynamic surface tension of xylem sap lipids.

The surface tension of xylem sap has been traditionally assumed to be close to that of the pure water because decreasing surface tension is thought to increase vulnerability to air seeding and embolism. However, xylem sap contains insoluble lipid-based surfactants, which also coat vessel and pit membrane surfaces, where gas bubbles can enter xylem under negative pressure in the process known as air seeding. Because of the insolubility of amphiphilic lipids, the surface tension influencing air seeding in pit pores is not the equilibrium surface tension of extracted bulk sap but the local surface tension at gas-liquid interfaces, which depends dynamically on the local concentration of lipids per surface area. To estimate the dynamic surface tension in lipid layers that line surfaces in the xylem apoplast, we studied the time-dependent and surface area-regulated surface tensions of apoplastic lipids extracted from xylem sap of four woody angiosperm plants using constrained drop surfactometry (CDS). Xylem lipids were found to demonstrate potent surface activity, with surface tensions reaching an equilibrium at ~25 mN/m and varying between a minimum of 19 mN/m and a maximum of 68 mN/m when changing the surface area between 50 and 160% around the equilibrium surface area. It is concluded that xylem lipid films in natural conditions most likely range from nonequilibrium metastable conditions of a supersaturated compression state to an undersaturated expansion state, depending on the local surface areas of gas-liquid interfaces. Together with findings that maximum pore constrictions in angiosperm pit membranes are much smaller than previously assumed, low dynamic surface tension in xylem turns out to be entirely compatible with the cohesion-tension and air-seeding theories, as well as with the existence of lipid-coated nanobubbles in xylem sap, and with the range of vulnerabilities to embolism observed in plants.
Marine heat waves threaten kelp forests

Marine kelp forests, among the most productive ecosystems on our planet, are in danger. The increase in the frequency and intensity of extreme climatic events such as marine heat waves is compromising kelp forests' capacity to produce goods and services (such as biomass of commercial fisheries, coastal protection, nutrient cycling, carbon sequestration, and recreational opportunities) that are worth billions of dollars to humanity. However, despite increasing climate-change advocacy and the overwhelming evidence demonstrating social and ecological impacts of climate change, political denial and inaction are jeopardizing society's ability to respond adequately to the multifaceted consequences of the accelerating pace of climate-driven loss of marine forests.
Knocking out barriers to engineered cell activity

CRISPR-Cas9 gene-edited T cells show safety and long-term engraftment in humans Engineered T cell therapies are revolutionizing cancer treatment by achieving long-lasting remission in blood-related cancers, such as leukemia and lymphoma. These therapies involve removal of patient T cells, “reprogramming” them to attack cancer cells, and then transferring them back into the patient. Targeted gene inactivation (knockout) using CRISPR-Cas9 can enhance T cell activity (1, 2) and has the potential to expand cell therapy applications. Until now, it has been unknown whether CRISPR-Cas9–edited T cells would be tolerated and thrive once reinfused into a human. On page 1001 of this issue, Stadtmauer et al. (3) present data from a phase 1 clinical trial (designed to test safety and feasibility) on the first cancer patients treated with CRISPR-Cas9–modified T cells. The findings represent an important advance in the therapeutic application of gene editing and highlight the potential to accelerate development of cell-based therapies.
Changes in Gait Parameters and Gait Variability in Young Adults during a Cognitive Task While Slope and Flat Walking

This study investigates the effects of a cognitive task while walking on a slope or a flat surface on gait parameters and gait variability in young adults. The participants consisted of thirty healthy young subjects. They were instructed to walk on a slope or on a flat surface while performing or not performing a cognitive task, which involved speaking a four-syllable word in reverse. A wearable inertia measurement unit (IMU) system was used to measure spatiotemporal parameters and gait variability. Flat gait (FG) while performing the cognitive task (FGC) and uphill gait (UG) while performing the cognitive task (UGC) significantly altered stride times, gait speeds, and cadence as compared with FG and UG, respectively. Downhill gait (DG) while performing the cognitive task (DGC) caused no significant difference as compared with DG. Gait variability comparisons showed no significant difference between UGC and UG or between FGC and FG, respectively. On the other hand, variabilities of stride times and gait speeds were significantly greater for DGC than DG. FGC and UGC induce natural changes in spatiotemporal gait parameters that enable the cognitive task to be performed safely. DGC should be regarded as high complexity tasks involving greater gait variability to reduce fall risk.
Growth differentiation factor 15 is increased in stable MS

Objective To assess whether serum concentrations of the anti-inflammatory cytokine growth differentiation factor 15 (GDF-15) differ in patients with highly active multiple sclerosis (MS) vs patients with stable MS and healthy controls (HCs). Methods GDF-15 concentrations were measured by ELISA in serum and CSF in a cross-sectional cohort of patients with MS, patients with other inflammatory neurologic diseases (OIND), patients with noninflammatory neurologic diseases (NIND), and healthy controls (HC). Serum GDF-15 concentrations were measured in a longitudinally sampled cohort of clinically and radiologically well-characterized patients with MS and corresponding controls. Results Cross-sectionally measured median serum GDF-15 concentrations were significantly higher in patients with OIND (n = 42) (600 pg/mL, interquartile range [IQR] = 320–907 pg/mL) compared with HCs (n = 29) (325 pg/mL, IQR = 275–419 pg/mL; p = 0.0007), patients with NIND (n = 46) (304 pg/mL, IQR = 245–493 pg/mL; p = 0.0002), or relapsing MS (n = 42) (356 pg/mL, IQR = 246–460 pg/mL; p = 0.0002). CSF and serum concentrations of GDF-15 were correlated (r = 0.41, 95% CI = 0.25–0.56, p < 0.0001). In a longitudinally sampled cohort of patients with MS (n = 48), deeply phenotyped with quantitative clinical and MRI assessments, mean GDF-15 concentrations were significantly higher in patients with a stable disease course (405 pg/mL, SD = 202) than in patients with intermittent MRI activity (333 pg/mL, SD = 116; p = 0.02). Conclusions Serum GDF-15 concentrations are increased in patients with MS with a stable disease course. These data suggest that GDF-15 may serve as a biomarker for disease stability in MS.
Dentistry, e-health and digitalisation: A critical narrative review of the dental literature on digital technologies with insights from health and technology studies.

OBJECTIVE To overview current developments in e-health and digitalisation in dentistry and identify gaps in the dental literature on this topic; Basic research design: a critical narrative review of published articles and relevant online materials; Results: Four themes are identified as characterising the current dental literature on e-health and digitalisation: 1) the impact of digitalisation on dental surgeries, 2) digital technology and practice management, 3) digitalisation beyond the dental surgery and in dentist-patient communication, and 4) digital technology and education. However, gaps remain in our understanding of the impact of digital technology on dental practice, particularly in relation to its ethical considerations. Following the example of the wider medical literature, the review introduces the field of critical digital health studies and identifies areas for future investigation and exploration based on its four characteristics: devices and software, data materialisation, data practices and data mobilities; Conclusion and Clinical significance: Digital technology is changing clinical practice and patient care. Dentistry needs to expand its understanding of how dental apps, digital workflow models and digital health information are transforming and disrupting dental practice in order to anticipate how this digital shift will impact on dentistry. The emerging field of critical digital health studies can signpost ways to improve research and practice on the topic in the future.
Multivariate analysis reveals activation-primed fibroblast geometric states in engineered 3D tumor microenvironments

Fibroblasts are a heterogeneous group of cells comprising subpopulations that have been found to be activated in the stromal microenvironment that regulates tumor initiation and growth. The underlying mechanisms of such selective activation of fibroblasts are not understood. We propose that the intrinsic geometric heterogeneity of fibroblasts modulates the nuclear mechanotransduction of signals from the microenvironment, resulting in their selective activation. To test this, we developed an engineered 3D fibroblast tumor coculture system and used high resolution images to quantify multiple cell geometry sensitive nuclear morphological and chromatin organizational features. These features were then mapped to activation levels as measured by the nuclear abundance of transcription cofactor, megakaryoblastic leukemia, and protein levels of its target, αSMA. Importantly, our results indicate the presence of activation-“primed” cell geometries that present higher activation levels, which are further enhanced in the presence of stimuli from cancer cells. Further, we show that by enriching the population of activation-primed cell geometric states by either increasing matrix rigidity or micropatterning primed cell shapes, fibroblast activation levels can be increased. Collectively, our results reveal important cellular geometric states that select for fibroblast activation within the heterogenous tumor microenvironment.
Extensive Genomic Rearrangements along with Distinct Mobilome and TALome are Associated with Extreme Pathotypes of a Rice Pathogen

Abstract Xanthomonas oryzae pv. oryzae (Xoo) is a serious pathogen of rice which displays tremendous interstrain variation. The emergence of highly-virulent strains of Xoo is a major threat to rice cultivation. Evolutionary insights into genome dynamics of highly virulent strains as compared with the less-virulent ones are crucial for understanding the molecular basis of exceptional success of Xoo as a highly evolved plant pathogen. In the present study, we report complete genome sequence of Xoo strains with extreme-virulent pathotypes (XVPs) characterized based on their reaction toward ten resistance (Xa) genes. One strain, IXO1088, can overcome resistance mediated by all the ten resistance genes while the other strain IXO704 cannot overcome any of them. Interestingly, our investigation revealed that XVPs display dramatic variation in the genome structure with numerous rearrangements/inversions. Moreover, XVPs also possess distinct transposon content and prophage elements that may provide genomic flux required for the acquisition of novel gene cassettes and structural changes in the genome. Interestingly, analysis of transcription activator-like effector proteins, which are major virulence determinants of Xanthomonas pathogen show marked variation in the transcription activator-like effector content and DNA binding domain of tal genes. Overall, the present study indicates the possible role of mobilomes and repetitive elements in major structural and sequence alterations, which may be leading to the emergence of novel and extreme pathotypes. The knowledge and resource of XVPs will be invaluable in the further systematic understanding of evolution and management of variant pathotypes of Xoo.
ICENET: A Semantic Segmentation Deep Network for River Ice by Fusing Positional and Channel-Wise Attentive Features

River ice monitoring is of great significance for river management, ship navigation and ice hazard forecasting in cold-regions. Accurate ice segmentation is one most important pieces of technology in ice monitoring research. It can provide the prerequisite information for the calculation of ice cover density, drift ice speed, ice cover distribution, change detection and so on. Unmanned aerial vehicle (UAV) aerial photography has the advantages of higher spatial and temporal resolution. As UAV technology has become more popular and cheaper, it has been widely used in ice monitoring. So, we focused on river ice segmentation based on UAV remote sensing images. In this study, the NWPU_YRCC dataset was built for river ice segmentation, in which all images were captured by different UAVs in the region of the Yellow River, the most difficult river to manage in the world. To the best of our knowledge, this is the first public UAV image dataset for river ice segmentation. Meanwhile, a semantic segmentation deep convolution neural network by fusing positional and channel-wise attentive features is proposed for river ice semantic segmentation, named ICENET. Experiments demonstrated that the proposed ICENET outperforms the state-of-the-art methods, achieving a superior result on the NWPU_YRCC dataset.
The helpfulness of online reviews

Online reviews are increasingly available for a wide range of products and services in e-commerce. Most consumers rely heavily on online reviews when making purchase decisions, so an important topic is that of understanding what makes some online reviews helpful in the eyes of consumers. Researchers have demonstrated the benefits of the presence of customer reviews to an online retailer, however, few studies have investigated how images in review content and the facial expressions of reviewers’ avatars influence the judgment of online review helpfulness. This study draws on self-construal theory, attribution theory and affect-as-information theory to empirically test a model of the interaction effects of images in review content and the facial expressions of reviewers’ avatars on online review helpfulness. Furthermore, the purpose of this paper is to identify an underlying mechanism of causal attribution toward store performance on the above effects. ,This study conducted two online experiments. Study 1 is a 2 (images in review content: one person with a product vs a group of people with a product) ×2 (facial expression of the reviewer’s avatar: happy vs angry) between-subjects design. Study 2 is a 3 (image: product alone vs one person with a product vs a group of people with a product) ×2 (facial expression of the reviewer’s avatar: happy vs angry) ×3 (valence of the review: positive vs negative vs neutral) between-subjects design. ,The results indicate that when consumers were exposed to a happy-looking avatar, they were likely to express higher perceptions of online review helpfulness in response to an image showing a group of people in a restaurant than they would for an image of one person in the same situation. In contrast, when consumers were exposed to an angry-looking avatar, their perceptions of online review helpfulness did not differ in response to images of either a group of people or of one person. Furthermore, cause attribution toward store performance mediated the interaction between images in content of reviews and the facial expression of a reviewer’s avatar on the perceptions of online review helpfulness. ,The authors provide insights into how to develop guidelines on how online reviews should be written so that readers perceive them to be helpful, and how to design effective reward mechanisms for customer feedback. ,Compared with previous studies, this study provides further contributions in three ways. First, it contributes to the literature on review content by showing which images in reviews are deemed to be helpful. Second, it extends previous findings from the literature relating to online peer reviews by demonstrating the importance of facial expressions in reviewers’ avatars (i.e. happy vs angry) when explaining helpfulness, rather than the strength of purchase intent. Third, this study contributes by further highlighting a novel mechanism which shows that a causal attribution toward store performance motivates the perceptions of online review helpfulness.