Miracle-dz/UniFAR-FTUs huggingface dataset:
Retroviral Rem protein requires processing by signal peptidase and retrotranslocation for nuclear function

Mouse mammary tumor virus (MMTV) is a complex murine retrovirus that encodes an HIV Rev-like export protein, Rem, from a doubly spliced version of envelope (Env) mRNA. Previously, the N-terminal 98-amino acid sequence of Rem, which is identical to Env signal peptide (SP), and full-length Rem were shown to be functional in a reporter assay that measures a postexport function. Here we show that MMTV-infected cells or cells transfected with rem or env cDNAs express SP, which is the active component in the reporter assay. Uncleaved Rem was partially glycosylated, but mutations in both glycosylation sites within the C terminus prevented Rem function. Mutations that reduced Rem or Env cleavage by signal peptidase greatly reduced SP levels and functional activity in the reporter assay and allowed accumulation of the uncleaved protein. Fluorescence microscopy revealed that GFP-tagged cleavage-site mutants are unstable and lack fluorescence compared with wild-type Rem, suggesting improper folding. Proteasome inhibitors allowed accumulation of uncleaved Rem relative to SP and increased reporter activity, consistent with SP retrotranslocation and proteasome escape before nuclear entry. Expression of a dominant-negative p97 ATPase did not alter levels of unprocessed Rem and SP but decreased reporter activity, suggesting p97-facilitated retrotranslocation of SP. Our results provide an example of a SP that is processed by signal peptidase and retrotranslocated to allow nuclear localization and function.
New SEC-DED (Single Error Correcting-Double Error Detecting) Code Implementation in WSNs (Wireless Sensor Networks)

Failures will be occur inevitably because Wireless Sensor Networks (WSNs) are randomly deployed in a hostile nature environment. Therefore, considering faults in WSNs is essential when we design WSN. In this paper, a SEC-DED (Single Error Correcting-Double Error Detecting) code and the implementing method is presented. Proposed code scheme is suitable for the WSN. Also, this paper classifies fault model in the sensor node. Proposed SECDED code has improved area-overhead more than 50% compare with conventional SEC-DED codes.
Early and late life cognitive activity and cognitive systems in old age

Little is known about the relative benefits of cognitively stimulating activities at different points in the lifespan. In a cohort of 576 older persons without dementia, we assessed current and past (childhood, young adulthood, middle age) frequency of cognitive activity; availability of cognitively stimulating resources in the home in childhood and middle age; and 5 domains of cognitive function. Past cognitive activity and cognitive resources were positively correlated with both current cognitive activity and current cognitive function. The association with cognitive function was reduced after controlling for current cognitive activity, however. Current cognitive activity was associated with better cognitive function, especially semantic memory and perceptual speed, even after controlling for past activity. The results suggest that past cognitive activity contributes to current cognition principally through its association with cognitive activity in old age. (JINS, 2005, 11, 400–407.)
Effect of environmental temperature on the degree of unsaturation of depot fats of pigs given different amounts of food.

1 Measurements were made of the iodine value (IV) of fat from seven sites on pigs which had been kept at 5, 13 or 23 oC during their growth from 20 to 90 kg. At each temperature the pigs were given one of three levels of daily food intake. 2 The IV of the outer back fat exceeded that of the inner by 7.7 units at the shoulder, 6.8 units at the midback and 2.4 units at the rump. 3 Increasing daily food intake significantly depressed the IV of fat only at the shoulder sites. 4 The IV of fat at all sites fell with increasing temperature by an amount ranging from 0.22 units per 1 oC at the midback to 0.55 units per 1 oC in the inner shoulder fat. The IV of perinephric fat fell by 0.39 units per 1 oC increase in temperature; that this fat was thus affected suggests that the effect of environmental temperature on fat composition is not conditioned by its effect on local tissue temperature. 5 It was calculated that these effects arose largely by changes in the proportions of fatty acids synthesized de novo; the exogenous fat consumed could have made only a small contribution.
SERUM ANTI-ZONA PELLUCIDA ANTIBODIES IS CORRELATED WITH ESTRADIOL LEVEL IN WOMEN WITH IN VITRO FERTILIZATION FAILURE

Objective: In vitro fertilization (IVF) is one of the several assisted reproductive technologies (ART) used to aid infertile couples to conceive a child. Many factors affecting the rate of success of IVF cycle. The aim of the present study was to assess the correlation of the presence of anti-zona pellucida antibodies (AZPA) and the outcome of IVF. AZPA also was correlated with hormone levels in women with IVF failure. Methods: Sixty women undergo IVF were participated in the study. Women group that had conceived from IVF are expressed as "pregnant group" while women who hadn’t are expressed as "failure group". All groups had treatment regimen depending on parameters. Women with IVF failure were devided into subgroups according to the total gonadotrophin dosage, duration of treatment, and oocyte yield. Results: Results revealed that there is the insignificant difference between pregnant and failure groups in serum AZPA level. The results showed a significant higher estradiol (E2) (p=0.024), anti-mullerian (AMH) (p=0.006), and lower prolactin (PRL) (p=0.002) in pregnant in comparing with failure group. Correlations studies indicated a significant correlation between AZPA and AMH in pregnant group, and there is a significant correlation between AZPA and E2 hormone in the failure group. Conclusion : It can be concluded that serum AZPA level does not differ between women who had success or failure IVF. However, in women with failure pregnancy in IVF, AZPA is positively correlated with E2 and its negatively correlated with PRL. In pregnant group, AZPA is positively correlated with AMH. Keywords: In vitro fertilization, Estradiol, Anti-zonapellucida antibodies
Transferring qualities from horseback riding to design

We see more and more attempts to design for bodily experiences with digital technology, but it is a notably challenging design task. What are the possible bodily experiences we may aim to design for, and how can we characterise them? By analysing a horseback riding experience, we came to identify the following themes: (1) how certain kinds of bodily experiences are best understood through experiencing them yourself -- the bodily ways of knowing, (2) how rhythm and balance create for particularly strong physical experiences of this kind, (3) how movement and emotion coincide in these experiences, (4) how the movement between seeing our own bodies as objects vs experiencing in and through our bodies is one of the ways we come to learn the language of expressing and understanding bodily action, and (5) how this in turn lets us describe the sensitive and delicate relationship of wordless signs and signals that represent, in the case described, two bodily agents -- a human and a horse. When the human-horse relationship is really successful, it can be described as rare moments of becoming a centaur. We translate these themes into design considerations for bodily interactions.
Experimental Validation of a SAR-Based RFID Localization Technique Exploiting an Automated Handling System

The synthetic aperture radar (SAR) approach has attracted a considerable interest in the context of phase-based techniques for the localization of UHF-RFID passive tags. In this letter, the results of an extensive experimental activity are presented, when the reader antenna is moved in front of a set of static passive UHF-RFID tags by means of a planar handling system. Measured performance is evaluated with respect to several system parameters: tag number and typology, tag reciprocal distance and orientation, trajectory and speed of the moving reader antenna. It is shown that the SAR processing can guarantee an accurate two-dimensional localization of multiple tags, with a localization error comparable to the size of typical commercial tags, by using a single reader antenna and without the need for any reference tag.
Cellular proteins associated with the E5 oncoprotein of human papillomavirus type 16.

Human papillomavirus type 16 (HPV-16) is strongly implicated in the aetiology of premalignant and malignant disease of the cervix. The mechanisms by which HPV-16 induces cell transformation have been the subject of intense investigation for several years. The viral genome encodes three oncoproteins, E5, E6 and E7, but until recently only E6 and E7 were subjected to such scrutiny. The papillomaviruses exhibit strongly conserved genetic organisation [ 11 and the genes of bovine papillomavirus (BPV) have comparable counterparts in HPV-16. Much of our understanding of the action of HPV-16 E5 has come from studies of BPV E5. However, the properties of these analogous proteins do differ. BPV E5 is responsible for the major transforming activity of the virus whereas the major transforming activity of HPV-16 occurs through the action of the E6 and E7 proteins [2,
Depression, anxiety, and social disability show synchrony of change in primary care patients.

OBJECTIVES The purposes of this study were to (1) characterize the social disability associated with the common psychiatric illnesses of primary care patients in terms of role dysfunction (self-care, family role, social role, occupational role) and (2) establish whether severity of psychiatric illness and disability level show synchrony of change. METHODS A two-stage sample design was employed. In the first stage, 1994 consecutive attenders of 25 general practitioners were screened on psychiatric illness by their physicians and with the General Health Questionnaire. A stratified random sample (n = 285) with differing probabilities was selected for a second-stage interview. Patients with psychiatric symptoms were reinterviewed 1 and 3.5 years later (n = 143). RESULTS (1) Disability level among patients was increased (moderately for depression, mildly for anxiety) and was associated with severity of psychiatric illness. (2) Most disability was found in occupational and social roles. (3) Change in severity of psychiatric illness was concordant with change in level of disability and was largely invariant across diagnosis (depression, anxiety, mixed anxiety/depression). At follow-up, disability among improved patients had returned to normal levels. CONCLUSIONS Psychiatric illness in primary care patients is associated with mild to moderate disability, and severity of psychiatric illness and disability show synchrony of change.
Plaque Regression and Stability Evaluated by Intravascular Ultrasound and Coronary Angioscopy: A Review

Although atherosclerosis seems to be continuously progressive and irreversible, it has been being demonstrated that substantial regression or stabilization of atherosclerotic lesions can occur by some interventions improving its determinants. However, the concepts of plaque regression or stabilization are not so new. It has been reviewed that the first reported observation of plaque regression could be found in the 1920s (Wissler, 1976), in which switching cholesterol-fed rabbits to low-fat chow over 2–3 years resulted in arterial lesions becoming more fibrous with a reduced lipid content. Later on, as the earliest prospective studies, a couple of previous studies in the 1950s have shown distinctive facts of plaque shrinkage or favorable change of its tissue components due to special diet therapy or some specific medications. In 1957 Friedman M et al. documented a result from prospective, interventional study demonstrating substantial shrinkage of atherosclerotic lesions performed in cholesterol-fed rabbits (Friedman, 1957). The dietary regimen raised total plasma cholesterol to around 1,000 mg/dl, and then animals received intravenous injections of phosphatidylcholine. They reported that after less than a week or so the size of the plaques as well as cholesterol stores within the arterial wall were significantly reduced. This striking report with some following supportive studies and reviews (Wissler, 1976; Armstrong, 1976; Malinow, 1983) has been surprisingly ignored for long years because of some beliefs regarding distinctive persistent characteristics of atherosclerosis, the negative history of which was reviewed by Stein Y, et al (Stein , 2001). However, various additional facts of plaque regression by some interventions have been demonstrated in other type of animal studies (Maruffo , 1968; Armstrong , 1970) since then without strong interests among general cardiologists. The first prospective study demonstrating plaque regression in humans might be the one documented by Ost CR, et al. in 1967(Ost, 1967). In the study, approximately 10% of patients treated with niacin showed improved femoral angiograms. Numerous additional larger trials of lipid lowering have then shown angiographic evidence of regression. However, though statistically significant, the resulted effects were very small (Brown, 1993), in which the improvement of percent stenosis of lumen were at most 2%. Accumulations of clinical evidences demonstrating the benefits of lipid-lowering in clinical outcome therefore yield
The Importance of Illness Perception in End‐Stage Renal Disease: Associations with Psychosocial and Clinical Outcomes

Illness perceptions refer to organized beliefs surrounding the symptoms, consequences, time course, controllability, and causes of an illness. Illness perceptions have been shown to predict a range of psychosocial and clinical outcomes in patients with ESRD including depression, nonadherence, and even survival. Accordingly, personal illness beliefs are novel yet potentially modifiable prognostic factors. Studies are required to assert whether illness perception‐based interventions deliver the promise of improved subjective and clinical well‐being in patients with ESRD.
The prevalence of occupational stress as a non-auditory effect of noise among palm oil mill workers in 7 sections of two selected mills

Palm Oil Mills are among the nosiest industrial workplaces, whereby excessive noise poses a threat to the health of organization and cause serious consequences. Excessive noise exposure gradually affects auditory and non-auditory aspects of health. The set symptoms are referred to as occupational stress, having a direct impact on concentration, thereby reducing the efficiency and productivity levels of organization. Therefore the objective of this study was to determine and the prevalence of occupational stress among Oil Palm Mill Workers in seven sections of two selected mills The study’s scope was on the non-auditory effects of excessive noise while . The participants were 62 workers of two selected Palm Oil mills. They were chosen through proportional stratified sampling. Based on the objective of this study the environmental sound levels were measured in seven different section while on the other hand O’Donnell inventory was utilized as a tool to determine the stress level. Evidence shows that Physiological and performance effects are the two most important body reflexes affected by high exposure risky noise levels. Noise as an occupational factor contributes to high occupational stress levels
Functional Genomics Reveals Extended Roles of the Mycobacterium tuberculosis Stress Response Factor σH

ABSTRACT Mycobacterium tuberculosis is one of the most successful pathogens of humankind. During infection, M. tuberculosis must cope with and survive against a variety of different environmental conditions. Sigma factors likely facilitate the modulation of the pathogen's gene expression in response to changes in its extracellular milieu during infection. σH, an alternate sigma factor encoded by the M. tuberculosis genome, is induced by thiol-oxidative stress, heat shock, and phagocytosis. In response to these conditions, σH induces the expression of σB, σE, and the thioredoxin regulon. In order to more effectively characterize the transcriptome controlled by σH, we studied the long-term effects of the induction of σH on global transcription in M. tuberculosis. The M. tuberculosis isogenic mutant of σH (Δ-σH) is more susceptible to diamide stress than wild-type M. tuberculosis. To study the long-term effects of σH induction, we exposed both strains to diamide, rapidly washed it away, and resumed culturing in diamide-free medium (post-diamide stress culturing). Analysis of the effects of σH induction in this experiment revealed a massive temporal programming of the M. tuberculosis transcriptome. Immediately after the induction of σH, genes belonging to the functional categories “virulence/detoxification” and “regulatory proteins” were induced in large numbers. Fewer genes belonging to the “lipid metabolism” category were induced, while a larger number of genes belonging to this category were downregulated. σH caused the induction of the ATP-dependent clp proteolysis regulon, likely mediated by a transcription factor encoded by Rv2745c, several members of the mce1 virulence regulon, and the sulfate acquisition/transport network.
Effectiveness of an alternating pressure air mattress for the prevention of pressure ulcers.

BACKGROUND studies of the effectiveness of alternating pressure air mattresses (APAMs) for the prevention of pressure ulcers are scarce and in conflict. OBJECTIVE evaluating whether an APAM is more or equally effective as the standard prevention. DESIGN randomised controlled trial. SETTING AND SUBJECTS patients admitted to 19 surgical, internal, or geriatric wards in seven Belgian hospitals were included if they were in need of prevention of pressure ulcers. To define this need, two methods were used randomly: the Braden Scale or the presence of non-blanchable erythema (NBE). METHODS 447 patients were randomised into either an experimental or a control group. In the experimental group, 222 patients were lying on an APAM (Alpha-X-Cell, Huntleigh Healthcare, UK). In the control group, 225 patients were lying on a visco-elastic foam mattress (Tempur, Tempur-World Inc., USA) in combination with turning every 4 hours. Both groups had identical sitting protocols. RESULTS there was no significant difference in incidence of pressure ulcers (grade 2-4) between the experimental (15.6%) and control group (15.3%) (P = 1). There were significantly more heel pressure ulcers in the control group (P = 0.006). There was an interaction effect between the risk assessment method and preventive measures for the development of all pressure ulcers and sacral pressure ulcers. CONCLUSION fewer patients developed heel pressure ulcers on an APAM. Patients identified as being in need of prevention based on the presence of NBE had a tendency to develop fewer pressure ulcers on an APAM. Patients identified as being in need of prevention, based on the Braden Scale, appeared to develop more sacral pressure ulcers on an APAM.
Development of audio-visual speech corpus toward speaker-independent Japanese LVCSR

In the speech recognition literature, building corpora for Large Vocabulary Continuous Speech Recognition (LVCSR) is quite important. In addition, in order to overcome performance decrease caused by noise, using visual information such as lip images is effective. In this paper, therefore, we focus on collecting speech and lip-image data for audio-visual LVCSR. Audio-visual speech data were obtained from 12 speakers, each who uttered ATR503 phonetically-balanced sentences. These data were recorded in acoustically and visually clean environments. Using the data, we conducted recognition experiments. Mel Frequency Cepstral Coefficients (MFCCs) and eigenlip features were obtained, and multi-stream Hidden Markov Models (HMMs) were built. We compared the performance in clean condition to those in noisy environments. It is found that visual information is able to compensate the performance. In addition, it turns out that we should improve visual speech recognition for high-performance audio-visual LVCSR.
Enumerating Vertices of $0/1$-Polyhedra associated with $0/1$-Totally Unimodular Matrices

We give an incremental polynomial time algorithm for enumerating the vertices of any polyhedron $\mathcal{P}(A,\mathbf{1})=\{x\in\RR^n \mid Ax\geq \b1,~x\geq \b0\}$, when $A$ is a totally unimodular matrix. Our algorithm is based on decomposing the hypergraph transversal problem for unimodular hypergraphs using Seymour's decomposition of totally unimodular matrices, and may be of independent interest.
Cancer stem cell theory: pathologists' considerations and ruminations about wasting time and wrong evaluations.

The genomic revolution has changed the role of the pathologist. In daily practice, our work is no longer limited to reaching a correct diagnosis and we are asked to answer questions about the patient’s prognosis and treatment options through the evaluation of selected molecular targets (such as erbB2 for breast cancer) in tumour specimens. Thus, we have acquired a major role in the translation of novel gene findings from experimental model systems to their clinical application. There is overwhelming evidence that only a subset of cells within a tumour clone, …
A system and method for transmission of traffic sign board information to vehicles and relevance determination

The system described in this paper makes use of Bluetooth Low Energy (BLE) to broadcast traffic sign board information to vehicles. In the vehicle multiple sign board information is mapped to its display relative to each other. Further these signs are classified, as per the local traffic rules to help the driver. The paper describes algorithm to triangulate the position of a particular traffic sign board relatively based on message received by four Bluetooth radios in the vehicle from multiple sign boards. In addition paper has multi-level alerting step to classify the signs identified by the vehicle.