Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Epigallocatechin-3-Gallate (EGCG) on Cell Cycle Distribution and DNA Integrity of K562 Cells, A Human Chronic Myeloid Leukemia

Epigallocatechin-3-gallate (EGCG) is a naturally derived compound from green tea with high antioxidant activity and various anti-cancer properties. EGCG has been widely investigated worldwide. However, effects of EGCG on cell cycle of K562 have not been clearly stated elsewhere. This study was conducted with the aim to investigate the antiproliferative effect of EGCG on K562 human leukemic cells and its underlying mechanism of action on the cells. MTT assay was conducted to determine cytotoxicity effect of EGCG on the K562 cells. Meanwhile, cell cycle analysis and DNA damage on the cells were determined by Flow cytometry and Comet assay respectively. K562 cells were treated with EGCG at concentrations ranging from 0 to 100μg/ml for 48 hours. The results showed that EGCG effectively decreased the percentage of cell viability in a dose dependent manner. The IC10, IC25 and IC50 of EGCG on K562 cell lines were 5 ± 2.44 μg/mL, 10 ± 5.93 μg/mL and 50 ± 1.93 μg/mL, respectively. In cell cycle assay, EGCG has shown no significant effect (p>0.05) on the cell cycle of K562 cell line as compared to negative control, whereas Imatinib mesylate as the positive control showed cell cycle arrest at S phase in this cell line. Hence, EGCG can be verified as a non-cell cycle specific compound. In addition, EGCG was found to cause a significant increase (p<0.05) in tail moment value and percentage of DNA tail in K562 cell line, suggesting DNA damage as an early signal of EGCG induced cell cytotoxicity. In conclusion, by decreasing the cell viability and inducing DNA damage, EGCG showed promising potential as an alternative treatment for leukemia through non-cell cycle specific pathway and further investigation on other mechanisms of action of EGCG on the cells is recommended. DOI: http://dx.doi. org/10.17576/JSKM-2020-1801-08
Hematology and Plasma Chemistry Values for Production Tilapia (Oreochromis hybrid) Raised in a Recirculation System

Tilapia are a frequently aquacultured fish, yet little is known about their normal physiology and response to disease. To assess specific diseases in mammals, blood constituents are routinely analyzed and compared to previously determined standardized values. Research to establish hematologic values for normal healthy fish is required before blood analysis can be used for diagnostic purposes in fishes. This study determined hematology and plasma chemistry values for production tilapia (Oreochromis hybrids) raised in a recirculation system. Using standard clinical techniques, the following hematologic parameters were determined: PCV (packed cell volume), plasma protein and MCV (mean cell volume) values; and erythrocyte, leukocyte, lymphocyte (small and large), neutrophil, monocyte, eosinophil, thrombocyte-like-cell, and thrombocyte numbers. Additionally, the following plasma chemistry values were determined: total protein, albumin, globulin, creatinine, total bilirubin, ALP (alkaline phosphatase), AST (aspartate aminotransferase), sodium, potassium, chloride, calcium, phosphorus, magnesium, glucose, cholesterol, ammonia and osmolality. Analysis of blood parameters can enhance production of hybrid tilapia by providing a means for the early detection of infectious diseases, and by assisting in the identification of sub-clinical conditions affecting production performance.
Del Apego Adulto a la Infidelidad Sexual: Un Análisis de Mediación Múltiple

A large proportion of couples experience infidelity issues, which substantially impact the quality of life of its members, their families, and society. Although numerous studies have found a positive association between insecure attachment and infidelity, less is known about the variables that may mediate this link. In this context, the objective of this study was to clarify the association between adult attachment and sexual infidelity, considering 3 possible mediators: participants’ satisfaction with their couple relationships and their perception of the positive and negative consequences of infidelity. A convenience sample of 403 university students was obtained at a public university in Santo Domingo, Dominican Republic. Participants’ age ranged from 18 to 69 years (M = 28.05, SD = 8.69). Adult attachment was measured with the Experiences in Close Relationships instrument, relationship satisfaction with the Couples Satisfaction Index, and sexual infidelity and the positive and negative consequences of infidelity with the Multidimensional Inventory of Infidelity. Results indicated that both avoidant and anxious attachment were positively correlated with sexual infidelity and that this association was largely mediated –according to a path analysis model with bootstrapping– by relationship satisfaction and a positive perception of the consequences of infidelity. These results are examined upon the basis of attachment theory. Lastly, future lines of research are proposed.
Circadian rhythmicity of body temperature and metabolism

ABSTRACT This article reviews the literature on the circadian rhythms of body temperature and whole-organism metabolism. The two rhythms are first described separately, each description preceded by a review of research methods. Both rhythms are generated endogenously but can be affected by exogenous factors. The relationship between the two rhythms is discussed next. In endothermic animals, modulation of metabolic activity can affect body temperature, but the rhythm of body temperature is not a mere side effect of the rhythm of metabolic thermogenesis associated with general activity. The circadian system modulates metabolic heat production to generate the body temperature rhythm, which challenges homeothermy but does not abolish it. Individual cells do not regulate their own temperature, but the relationship between circadian rhythms and metabolism at the cellular level is also discussed. Metabolism is both an output of and an input to the circadian clock, meaning that circadian rhythmicity and metabolism are intertwined in the cell.
Modelo de Fatores para Commodities e Cenários de Preços no Curto Prazo: o caso da soja

Resumo Este artigo analisa os cenários futuros de curto prazo para o preço da soja à vista. A abordagem é feita modelando os fatores não observáveis por processos estocásticos e inova ao incluir o componente sazonal. As variáveis de observação são os preços futuros (estrutura a termo de contratos futuros). Procede-se à estimação do modelo pelo filtro de Kalman. Os resultados mostram um cenário de curto prazo mais favorável a produtores e exportadores. Por outro lado, consumidores e importadores têm indicação de medidas de proteção necessárias para suas posições. A análise pode ser estendida a outras commodities dependentes da sazonalidade. Da mesma forma, o modelo pode ser empregado no apreçamento de derivativos, em que o ativo subjacente é o preço futuro.
Ligando Frugivoria e Dispersão de Sementes à Biologia da Conservação

Na década de 1970, Daniel H. Janzen, um dos grandes ecólogos tropicais, chamou a atenção para um problema de conservação muito mais sutil do que a extinção de espécies: a perda de interações bióticas em áreas tropicais sujeitas a perturbações de origem antrópica (Janzen, 1974). Desde então, e particularmente a partir da década de 1990, a comunidade científica passou a investigar de que maneira as várias interações bióticas que ocorrem em ambientes tropicais são afetadas pela degradação ambiental. Assim, a Biologia da Conservação deixou de enfocar apenas a preservação de áreas naturais e animais chamativos e desde então vem discutindo a conservação da integridade das interações entre espécies (Sechrest et al., 2000; Díaz et al., no prelo). Uma das grandes fronteiras dessa área de estudo é prever as conseqüências da alteração nas interações bióticas para as populações que interagem. As interações animal–planta, planta–planta e animal– animal são importantes para a produção de certos serviços à humanidade, além de serem críticas para a manutenção da integridade das comunidades onde CAPÍTULO 18
Corporate social responsibility and dynamic productivity change in the US food and beverage manufacturing industry

This study examines the relationship between corporate social responsibility (CSR) and dynamic productivity change of each input employed and investment undertaken in the US food and beverage manufacturing industry. We compute input‐ and investment‐specific dynamic Luenberger indicators and decompose them into the contributions of input‐ and investment‐specific dynamic technical inefficiency changes and dynamic technological changes. We then relate these indicators to an overall CSR measure and aspect‐specific CSR measures (governance, environment, and social). Our results confirm that the association between CSR and productivity change has different signs and effects on specific inputs or investments. We also find that only certain aspects of CSR participate in the association between CSR and dynamic productivity change and its components. [EconLit citations: C61, D24, L66, M14].
The Regulatory Framework in the Organization, Management and Production Efficiency

The basis for successful operation of industrial enterprises and the quality assurance of products quality management is based on a progressive regulatory framework and to ensure its respect in the production process. The role of standardization in the quality management of the products at the present stage is to strengthen the organic link with the quality standards, as well as the organizational possibilities of standardization on the combined efforts of enterprises to their targeted effects on quality at all stages of production and management levels. The Foundation of organizational-economic nature of the system of quality control is the normative-technical and methodological documentation, ensuring unity and regulating action according to the directions of the work. Assessment of product quality and process control specifies the State of the managed object and its deviations from the specified level. Building a system for the creation and production of high quality products in strict accordance with the requirements of normative documentation and meet consumer needs delivers increased efficiency of production and activities Enterprise. In order to impact on quality at all stages of production and management levels, the entire regulatory framework in the Organization, management and production efficiency is included in the standard, which is mandatory for all services and employees Enterprise. Compliance with the requirements of the standard significantly improve the quality and effectiveness of the functioning of the enterprise.
P147 Association Between Inflammatory Markers of Low Intention and Arterial Stiffness

Extracellular and biochemical changes in the vessel contribute to the stiffening, this process is determinant for the increase of the Pulse Wave Velocity (PWV) and subsequent increase of the central and brachial arterial pressures [1,2]. These vascular alterations are denominated of TOD (target organ damage) and represent a point of association between cardiovascular risk factors and cardiovascular events [2,3]. Chronic low-grade inflammation associated with endothelial dysfunction and increased number of biomarkers, such as ultra-sensitive C-reactive protein (hsCRP), cytokines such as interleukins, fibrinogen, platelets, leukocytes and hematocrit [4,5,6]. Methods A cross-sectional exploratory study on a representative population of a community in Salvador-Bahia-Brazil. The data came from a including 301 individuals. 150 were initially assessed from December 2016 to May 2019. PWV measurement for the carotid-femoral by an ATCor SphygmoCor, data not demonstrated in this poster. Blood samples were collected to biochemistry analysis, ADVIA1800® (SiemensHealthcare Japan/Canada). The committee for research on human was done. Results The data show a predominance of women (65%). Changes in leukocytes, platelets and hematocrit were more prevalent in men, as observed in Table 1. Mean values of changes in ultra-sensitive CRP values were higher in women (0.43) than in men (0, 25). Conclusion Studies correlate the markers evaluated in this study as positive predictive factors for arterial stiffening. Data from the literature show these preliminary changes present in the male population, as observed in our population. The cytokines IL-1, 6 and 18, the chemokines MCP-1 and 3 and the adhesion molecules VCAM, ICAM are being evaluated to better respond to these findings. Table 1 WBC HEMATOCRIT PLATELETS Column B vs Column A WBC vs WBC Ht WOMAN vs Ht MAN PLAT WOMAN vs PLAT MAN Unpaired t-test p-value 0.0004 0.0246 0.0337 p-value summary *** * * Significantly different (p < 0.05)? Yes Yes Yes One- or two-tailed p-value? Two-tailed Two-tailed Two-tailed t, df t = 4.840, df = 12 t = 2.980, df = 6 t = 2.397, df = 12 How big is the difference? Mean of column A 8950 −3.567 311.3 Mean of column B 5767 3.167 250.2 Difference between means (B - A) ± SEM −3183 ± 657.7 1.197 −61.09 ± 25.49 95% confidence interval −4616 to −1750 −6.496 to -0.6378 −116.6 to −5.553 R squared (eta squared) 0.6613 0.5968 0.3237
Development of NFC and IoT-enabled measurement devices for improving health care delivery of Indonesian children

In this research, a prototype of NFC and IoT-enabled medical measurement device aimed for infants between 0-5 years old is designed. The device is aimed to record data and transmit them to NFC tags on a greater system containing electronic medical record of Indonesian children, which is normally recorded in a paper card named Kartu Menuju Sehat (KMS). The device can measure five parameters: height, weight, temperature, heart rate, and blood oxygen (SpO2). The result is passed to a sensor fusion system to determine health status of the child, after which the data is transmitted to mobile KMS application owned by child’s guardian and health personnel. The system is tested on 12 infants of 12 months and below in two Indonesia’s community health centre Posyandu units. Based on the result, the system is able to measure five parameters at once and transmit it to the health personnel and child’s guardian, making it feasible to develop the prototype further into a fully functional medical device for use with electronic KMS.
Implications juridiques et éthiques des algorithmes d'intelligence artificielle dans le domaine de la santé

L'Intelligence Artificielle (IA) envahit nos quotidiens et le domaine de la sante notamment pour aider au diagnostic, faire des choix therapeutiques ou encore viser une medecine predictive de precision. Absente de la loi francaise de bioethique du 7 juillet 2011, l'IA fut tres presente lors des Etats Generaux accompagnant la revision de la loi en 2018. La profusion de guides ou recommandations ethiques sur l'IA (soft law), motivees par la necessite de conquerir la confiance des usagers, incite prealablement a se preoccuper de leur vigueur normative, en lien avec les textes juridiques promulgues depuis l'entree en vigueur le 25 mai 2018 du RGPD (reglement 2016/679/UE reglement general de protection des donnees personnelles). Une analyse conjointe de ces textes, des algorithmes d'IA deployes et d'applications concretes en sante permet de poser les principales questions ethiques et legales soulevees dans ce domaine : principe du consentement libre et eclaire du patient face a l'opacite des algorithmes, risques potentiels de discrimination dans l'acces au soin, interet public ou bien commun attendu de la recherche en comparaison des risques encourus par l'ouverture de l'acces aux donnees personnelles. Les reponses conduisent a des recommandations deontologiques ou reglementaires indispensables a la transparence des ces outils : protection drastique des donnees de sante, notamment genetiques, et de leurs utilisations, rigueur des pratiques de recherche pour produire des resultats reproductibles donc scientifiques, detection des biais avant certification des dispositifs de sante et explicitation du protocole d'information des patients.
Unpacking the Emotions behind TripAdvisor Travel Reviews: The Case Study of Gatlinburg, Tennessee

ABSTRACT This study investigated how tourists’ emotions about their travel experiences were presented through TripAdvisor travel reviews targeting Gatlinburg, Tennessee due to its historical and geographical importance as a tourist destination. After selecting five Gatlinburg tourist attractions based on their review quantity, we conducted a polarity analysis of each tourist attraction and performed sentiment analyses based on the classical emotion criteria of psychology (i.e., six basic emotions – anger, disgust, fear, joy, sadness, and surprise). The findings revealed that visitors tended to have favorable emotional responses toward the five attractions, and the results of polarity and sentiment analyses of respective attractions corresponded to their TripAdvisor ratings.
Gut Microbiota Composition and Metabolites as the new Determinants of Cardiovascular Pathology Development

Chronic noncommunicable diseases represent one of the key medical problems of the XXI century. In this group cardiovascular diseases (CVD) are known to be the leading cause of death which pathogenesis still has the potential to be more profoundly revealed in order to discover its yet unknown but essential factors. The last decades are marked by the active investigation into the gut bacterial role in the initiation and progression of CVD. The result of this investigation has been the appreciation of microbiome as the potentially new cardiovascular risk factor. The development of sequencing techniques, together with bioinformatics analysis allowed the scientists to intensively broaden the understanding of the gut microbiota composition and functions of its metabolites in maintaining the health and the development of atherosclerosis, arterial hypertension and heart failure. The interaction between macro- and microorganisms is mediated through the variety of pathways, among which the key players are thought to be trimethylamine-N-oxide (TMAO), short chain fatty acids (SCFA) and secondary bile acids. TMAO is known due to its role in atherosclerosis development and the increase in major cardiovascular events. In the majority of research SCFA and secondary bile acids have demonstrated protective role in CVD. The great attention is being paid to the role of lipopolysaccharide of gram negative bacteria in the development of systemic low-grade inflammation due to the metabolic endotoxemia which contributes to the progression of CVD. The described interactions draw attention to the opportunity to influence on the certain mechanisms of CVD pathogenesis through the modulation of microbiota composition and function. The review is aimed at highlighting the current data about the mechanisms by which the gut microbiota and its metabolites may increase cardiovascular risk and events rate as well as discussing the existing results and future perspective of bacterial systemic effects modulation.
Temporal and Spatial Expression of a Gene for the Nuclear Protein Hgv2 in Embryos and Adults of the Ascidian Halocynthia roretzi

The pHgv20 cDNA clone encodes an ascidian embryonic nuclear protein, Hgv2, that is closely related to the amphibian histone‐binding protein N1. Genomic Southern blot analysis revealed the presence of two or more genes that hybridize with the Hgv2 probe under high‐stringency conditions, although it remains to be determined whether or not each of them is actively expressed. On Northern blots prepared from embryos, a single, 2.3‐kb Hgv2 mRNA was detectable during early stages of embryogenesis. The amount of Hgv2 mRNA gradually decreased after the 64‐cell stages. Northern, Western and immunohistochemical analyses showed that the Hgv2 protein was not expressed exclusively in the oocyte: small amounts of the 2.3‐kb mRNA and of the 83‐kDa Hgv2 protein were detectable in the branchial sac of adult organisms. Weak but specific immunohistochemical staining was observed in the spermatocytes and/or spermatogonia. An Hgv2‐specific antiserum reacted specifically with the 83‐kDa protein on the Western blot of the testis. These results suggest that Hgv2 functions not only in embryonic cells but also in sperm precursor cells and some somatic cells.
Can The Social Mission Model Improve the Students’ Interest?

This study aims to determine the effectiveness of entrepreneurship learning models based on social missions to advance entrepreneurial interest in vocational high school students. Experimental research use as a method, with 72 students participating in Class XII of SMK Negeri 1 Kudus. Data collection using questionnaires and learning outcomes. Data analysis using ANOVA and Scheffe test. The results showed that student learning outcomes with social mission learning models and conventional learning models have differences, where the social mission learning model is more useful to improve student learning outcomes and entrepreneurial interest. More than that, the social mission-based entrepreneurship learning model is needed in the curriculum. Entrepreneurship training through social mission will prepare students to become entrepreneurs, in addition to being more concerned about the environment and society. Future research needs to focus more on studying entrepreneurship learning curricula in vocational high schools.
Optical and geometrical aerosol particle properties over the United Arab Emirates

Abstract. One year of ground-based night-time Raman lidar observations has been analysed under the Optimization of Aerosol Seeding In rain enhancement Strategies (OASIS) project, in order to characterize the aerosol particle properties over a rural site in the United Arab Emirates. In total, 1130 aerosol particle layers were detected during the 1-year measurement campaign which took place between March 2018 and February 2019. Several subsequent aerosol layers could be observed simultaneously in the atmosphere up to 11 km. The observations indicate that the measurement site is a receptor of frequent dust events, but predominantly the dust is mixed with aerosols of anthropogenic and/or marine origin. The mean aerosol optical depth over the measurement site ranged at 0.37 ± 0.12 and 0.21 ± 0.11 for 355 and 532 nm, respectively. Moreover, mean lidar ratios of 43 ± 11 sr at a wavelength of 355 nm and 39 ± 10 sr at 532 nm were found. The average linear particle depolarization ratio measured over the course of the campaign was 15 ± 6 % and 19 ± 7 % at the 355 and 532 nm wavelengths, respectively. Since the region is both a source and a receptor of mineral dust, we have also explored the properties of Arabian mineral dust of the greater area of the United Arab of Emirates and the Arabian Peninsula. The observed Arabian dust particle properties were 45 ± 5 (42 ± 5) sr at 355 (532) nm for the lidar ratio, 25 ± 2 % (31 ± 2 %) for the linear particle depolarization ratio at 355 (532) nm, and 0.3 ± 0.2 (0.2 ± 0.2) for the extinction-related Ångström exponent (backscatter-related Ångström exponent) between 355 and 532 nm. This study is the first to report comprehensive optical properties of the Arabian dust particles based on 1-year long observations, using to their fullest the capabilities of a multi-wavelength Raman lidar instrument. The results suggest that the mineral dust properties over the Middle East and western Asia, including the observation site, are comparable to those of African mineral dust with regard to the particle depolarization ratios, but not for lidar ratios. The smaller lidar ratio values in this study compared to the reference studies are attributed to the difference in the geochemical characteristics of the soil originating in the study region compared to northern Africa.