Miracle-dz/UniFAR-FTUs huggingface dataset:
Genetic fingerprinting of Pseudomonas syringae pathovars using ERIC-, REP-, and IS50-PCR.

PCR fingerprinting using primers corresponding to repetitive (ERIC and REP) and insertion sequences (IS50) was investigated as a method to distinguish the pathovars of Pseudomonas syringae. After amplification of total DNA with the ERIC-, REP-, and IS50-PCR followed by agarose gel electrophoresis, most of the tested pathovars showed specific patterns of PCR products. The differences between the fingerprints among strains within a pathovar were small, with the exception of pathovars syringae, aptata, and atrofaciens. The fingerprints of the related pathovars savastanoi, phaseolicola, glycinea, morsprunorum, tabaci, lachrymans, and mori generated with the ERIC- and REP-primers were found to be very similar, showing the potential of this technique for taxonomical studies. In contrast, the IS50-PCR fingerprints of these pathovars were clearly distinguishable. The fingerprint patterns of a strain were highly reproducible with all three tested primer sets, also when whole cells were added to the reaction mixture. Thus, the PCR technique with the ERIC-, REP-, and IS 50-primers is a rapid, simple, reproducible, and low cost method to identify and classify strains of the Pseudomonas syringae pathovars.
Emerging Technologies: Cloud implementation on Small and Medium Enterprises

Cloud computing, one of the emerging technologies is a service offering widely popular in the world of business as it provides growth of key technologies like, low cost of CPU power and memory, high speed network facilitator, availability of virtualized systems management software with high performance and extensive reception of internet practice. It has provided extraordinary opportunity to all organizations and business enterprises, small and medium in particular, to enlarge their business with the use of scalable computing resources which are available at a very low cost. The aim of this paper is the study of concept of cloud computing and its high impacts on the success of various companies specially the small and medium enterprises. There is a case study presented in the paper to understand the benefits and confronts of deploying an application in IAAS environment.
Anti-inflammation and anti-fibrosis with PEGylated, apigenin loaded PLGA nanoparticles in chronic pancreatitis disease

Chronic pancreatitis (CP) is a disease that imposes great suffering on people’s lives with symptoms such as chronic abdominal pain, diabetes, impaired digestion, high risk of pancreas cancer, etc. However, treatment of CP is limited by the lack of effective therapies. In this work, apigenin, a drug that can inhibit pancreatic stellate cell fibrosis, is loaded into PEGylated PLGA nanoparticles to treat CP. The nanoparticles have a diameter around 160 nm and loading efficiency of 96 μg apigenin per mg PLGA nanoparticles. Our studies find that apigenin loaded nanoparticles can inhibit PSC growth and promote PSC apoptosis. To investigate the mechanism of apigenin in treating CP, PSCs with pro-inflammatory and pro-fibrotic responses are treated with apigenin loaded nanoparticles and analyzed with RT–PCR. Our study finds that apigenin loaded nanoparticles can reduce the expression of mRNAs associated with PSC pro-inflammation and pro-fibrosis (e.g. collagen 1A1 mRNA, fibronectin mRNA and IL-6 and IL-8 mRNAs). An in vivo study finds that apigenin loaded in PEGylate PLGA nanoparticles has a longer circulation time in mice than apigenin in its free form. Consistent with the in vitro study, the in vivo study also confirms the anti-inflammation and anti-fibrosis effects of PEGylated, apigenin loaded PLGA nanoparticles through reducing the inflammation and fibrosis associated mRNAs.
The distribution of xylose in the cells of ballistosporous yeasts - Application of high performance liquid chromatography without derivatization to the analysis of xylose in whole cell hydrolysates.

The presence or absence of xylose in the whole cell hydrolysates from all type strains of 41 known species of the genera Bullera, Sporidiobolus, and Sporobolomyces, and three strains of Itersonilia perplexans was examined using high performance liquid chromatography (HPLC) which makes itpossible to analyze the carbohydrates without derivatization. Of 16 species of the genus Bullera, 14 had xylose in the cells. The other two species, Bullera salicina and Bullera tsugae, had no xylose in the cells. None of the 25 species of the genera Sporidiobolus and Sporobolomyces had xylose in the cells. The lack of xylose in the cells of seven Sporobolomyces species with Q-9 as the major ubiquinone proves that these species are not yeast phases of certain species of the genus Itersonilia with Q-9, since both the yeast and filamentous phases of Itersonilia perplexans had xylose in the cells. These results show that the presence or absence of xylose in the cells is very useful as one criterion for the generic differentiation of Bullera and Sporobolomyces. The lack of xylose in the cells of B. salicina and B. tsugae indicates that these two species may be closely related to the genus Sporobolomyces. Our HPLC method, by which xylose in the whole cell hydrolysates can be analyzed without derivatization, is a useful way to differentiate taxa by this criterion.
INVESTIGATING THE EFFECTS OF DEMOGRAPHICS ON CUSTOMER ACCEPTANCE OF eBANKING IN DERA ISMIL KHAN, KPK, PAKISTAN: A SURVEY OF SAMPLE CLIENTELE -

eBanking and iBanking are the buzzwords among internet-savvy population of the world. It is happening not only in the advanced countries but also in the developing states like Pakistan. This is because on one hand the Information and Communication Technologies have become accessible both in terms of availability and prices and on the other hand due to the emergence of local Information technology professionals equipped with knowledge and command over the leading-edge technologies. However, research on the implementation of Information technology in the banking sector reports that the process is not automatic rather requires multi-disciplinary treatment with keeping the issues of customer acceptance on top. The customers of every state and city are different not only because of getting different eBanking digital facilities (digital-divide) but also due to the fact that every customer is demographically different from others. This research is about the effects of demographics on Customer Acceptance in Pakistan. The literature from developed states and developing states, including Pakistan has been analyzed to understand the problem and to develop a research model for testing in
Muscular Damage and Kidney Function in Rugby Players after Daily Whole Body Cryostimulation

Muscular damage, consequent to strenuous activities, could exceed the recovery potential of muscles and determine renal failure. Whole body cryostimulation is a cold-based therapy used to improve recovery or overcome fatigue symptoms. This study aimed to evaluate the effects of repeated sessions of cryostimulation on muscle damage, renal function, and their relationship. Serum samples, from 27 elite rugby players, under training, before and after 2 sessions/day of cryotherapy over 7 days, were tested for markers of muscular (creatine kinase, lactate dehydrogenase, and aspartate aminotransferase) and renal (creatinine, cystatin C) functions. eGFR was calculated with two formulas based on either serum creatinine concentration (MDRD) or serum creatinine and cystatin C concentrations (creatinine/cystatin C CKD EPI). Pre- and posttreatment differences were determined by Wilcoxon’s test; correlations were evaluated with Spearman’s test. Cryostimulation helped muscular recovery (increased lactate dehydrogenase activity and the stabile creatine kinase and aspartate aminotransferase activities). Creatinine was unaffected while cystatin C was increased . Creatinine-based eGFR was not affected by cryostimulation, while creatinine/cystatin C-based eGFR showed a slight decrease . eGFR and muscular biomarkers were not correlated, suggesting a real recovery effect of cryotherapy. Cystatin C seemed more sensible than creatinine in evaluating the kidney function.
Intravenous Ascorbic Acid (Vitamin C) Dose Needed in Critically Ill Patients with Sepsis and Septic Shock Post Cardiac Surgeries

Introduction: Sepsis, a syndrome of physiologic, pathologic, and biochemical abnormalities induced by infection, is a major public health concern, accounting for more than $20 billion (5.2%) of total US hospital costs in 2011. Sepsis is a life-threatening systemic inflammatory response that can result in multi-organ dysfunction and intractable hypotension. In critically ill patients, severe sepsis and septic shock are known as the main causes of mortality. Adequate fluid resuscitation, infection control, cardiovascular and respiratory supports are recommended for the management of severe sepsis and septic shock. Effects of ascorbic acid on hemodynamic parameters were evaluated in nonsurgical critically ill patients in limited previous studies. High-dose ascorbic acid in patients with burn injury decreased fluid and vasopressors’ requirements. Also, in patients with severe sepsis, high-dose intravenous ascorbic acid improved hemodynamic parameters. [2] Benha University Hospitals in the academic year of 2017. Patients were divided into 3 groups: • Group 1: Control (conventional management; IV fluids + antibiotics + vasopressors • Group 2: 3 grams/day. • Group 3: 6 grams/day. Conclusion: We suggested that the early use of intravenous vitamin C may prove to be effective in preventing progressive organ dysfunction including acute kidney injury and reducing the mortality of patients with severe sepsis and septic shock. This inexpensive and readily available intervention has the potential to reduce the global mortality from sepsis. Introduction Sepsis, a syndrome of physiologic, pathologic, and biochemical abnormalities induced by infection, is a major public health concern, accounting for more than $20 billion (5.2%) of total US hospital costs in 2011. [1] Sepsis is a life-threatening systemic inflammatory response that can result in multi-organ dysfunction and intractable hypotension. In critically ill patients, severe sepsis and septic shock are known as the main causes of mortality. Adequate fluid resuscitation, infection control, cardiovascular and respiratory supports are www.jmscr. igmpublication.org Index Copernicus Value: 79.54 ISSN (e)-2347-176x ISSN (p) 2455-0450 DOI: https://dx.doi. org/10.18535/jmscr/v7i2.148
Optimizing oral health: towards a tailored, effective and cost-effective dental care

Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva. nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible.
Genetics of Ustilago hordei: Fungicide-Resistance Mutants

Spontaneous and induced mutants of Ustilago hordei were characterized for resistance to benomyl, chloroneb, thiabendazole, and baytan. Auxotrophic mutants were detected by replica plating and fungicide resistance by gradient plating and testing transfers of the mutants. Fungicide resistance may be genetically determined and stable or nongenetic and unstable. Resistance to chloroneb and thiabendazole is controlled by more than one locus and is dominant, semidominant, or recessive. Loss of resistance and inability of stable mutants in the absence of the fungicide to compete with the wild-type strain could have important implications for fungal disease control.
Load-Balanced Data Dissemination for Wireless Sensor Networks: A Nature-Inspired Approach

The traditional many-to-one transmission pattern allows all sensor nodes to propagate their packets toward a single sink in wireless sensor networks, resulting in imbalanced energy depletion in the network. In this paper, we propose a data dissemination strategy named transmission with multiple load balancing schemes (TMLBSs) which utilizes a nature-inspired approach, ant colony optimization, to construct transmission paths for nodes in different places. The distinct characteristics of TMLBS are three load balancing schemes, which help to construct transmission paths formed into a path tree. The first one is the load decentralization scheme, which creates multiple path subtrees in the early stage and scatters the whole load to such path subtrees, so as to avoid excessive load concentration. The second one is the load maintenance scheme, which adopts an appropriate pheromone update mechanism to retain previous good paths, yielding excellent next-generation solutions. The last one is the load diversion scheme, which employs the heuristic factor to transfer traffic load to paths with light traffic load to eliminate poor solutions. Finally, extensive simulations are conducted to validate the effectiveness and advantages of the new transmission strategy.
The influence of refrigeration on Salmonella.

This study aimed to analyse the influence of refrigeration on Salmonella.Two register strains have been used – S. Enteritidis (D) (ATCC 13076) and S. Typhimurium (ATCC14028), each having a known load of 28*10, respectively 40*10. The two strains have been subjected to temperature variations as such: 1. -18°C for 1 h 45 min; 2. 2-4°C for 6 h after the first stage; 3. 2°C for 10 h after the second stage.The collected samples have been analyzed according to ISO 6579:2002. The results have shown that after quick refrigeration (-18°C for 1 h 45 min), the S. enteritidis and S. typhimurium microorganisms at the load of 22 log ufc/cm, respectively 44 log ufc/cm could not be noticed. The other two variations (2-4°C for 6 h and 2°C for 10 h) did not have any effect on the two Salmonella strains, no matter the UFC number used. We can affirm that the temperature of-18°C prevent the multiplication of microorganisms only if the microbial load is reduced, and once with increasing temperature the microorganisms have been highlighted.
Biological Activities Assessment of Centella asiatica (Linn.)

Biological activities of Centella asiatica were determined as cytotoxic activity–LD50 = 1926 μg. mL−1 using Artemia salina; mild insecticidal activity (40% mortality) against Rhyzopertha dominica; no activity against Tribolium castaneum and Callosobruchus analis; phytotoxicity (70% growth regulation at 1,000 μg.mL−1 and 10% growth regulation at 10 μg.mL−1) against Lemna minor. Mild antibacterial and antifungal activities were noted against Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans, while no activity was noted against Staphylococcus aureus and E. coli. Antioxidant performance was comparable to ascorbic acid, and analgesic and anti-inflammatory activities comparable with aspirin at 100 mg.kg−1b.w.
Evaluación de dos frecuencias de colecta de apitoxina extraída de colmenas de Apis mellifera L. durante la época estival en la Región de La Araucanía

El experimento se llevo a cabo desde el 7 de enero al 9 de marzo del 2006, en el Apiario del Centro Experimental Pillanlelbun de la Universidad Catolica de Temuco, ubicado a 15 km al norte de Temuco, Region de La Araucania, Chile. En este lugar se evaluo la produccion de apitoxina extraida de colmenas Apis mellifera (Hymenoptera:Apidae). Para la realizacion del experimento se conto con extractores de apitoxina del tipo canadiense, los que funcionan a traves de electroestimulacion. Se utilizaron tres colmenas para cada tratamiento y se evaluaron dos frecuencias de extraccion diferentes, cada 20 dias (T1) y cada 30 dias (T2). Las variables analizadas fueron: efecto en el peso de las colonias, mortalidad de abejas y cantidad de apitoxina extraida. No se encontraron diferencias significativas entre las variables estudiadas (peso colonias p = 0,127; mortalidad de abejas p = 0,827; y cantidad de apitoxina p = 0,507), por lo cual se concluye que es recomendable realizar extracciones cada 30 dias en comparacion a las extracciones cada 20 dias, pues es preferible realizar esta operacion mas distanciada en el tiempo a fin de disminuir costos de operacion y molestias a las abejas
Effects of protein-free energy supplementation on blood metabolites, insulin and hepatic PEPCK gene expression in growing lambs offered rice straw-based diet.

This study was conducted to investigate the effects of increasing levels of protein-free energy supplementation on blood glucose, urea nitrogen, insulin and gene expression of hepatic phosphoenolpyruvate carboxykinase (PEPCK) in growing lambs offered rice straw-based diet. Thirty-six male Hu lambs (3.5 months old) were divided into four equal groups according to body weight. All animals were fed rice straw ad libitum and supplemented with cornstarch at levels of 0 (control), 60, 120, and 180 g/day, respectively, along with 160 g/day of rapeseed meal. The trial lasted for 60 days with the first 15 days for adaptation. Body weight change and feed intake were recorded. Blood samples were taken at different time points after feeding at the end of the trial, and analyzed for blood glucose, total protein, urea nitrogen and insulin. Liver samples were collected and analysed for the mRNA abundance of hepatic PEPCK. Increasing cornstarch showed a low effect on rice straw intake, but increased average daily gain of lambs significantly ( P 0.05). Amounts of mitochondrial PEPCK (PEPCK-M) mRNA were not affected by the supplementation of starch at any level ( P > 0.05). These results indicate that proper energy supplementation increases the expression of PEPCK-C, and consequently gluconeogenesis and blood glucose increase, while excessive energy may have an inhibitory effect on gluco - neogenesis through insulin-involved mechanisms.
Risk-Based Decision Support Model for the Optimal Operation of a Smart Energy Distribution Company for Enabling Emerging Resources

In this paper, a risk-based decision support model is developed for a smart energy distribution company, enabling emerging resources like renewable energy sources, electric vehicles and demand response programs in a holistic approach. Because of the inherent uncertainties of these emerging resources, the conditional value-at-risk (CVaR) method is adopted to restrict the distribution company’s risk. A risk aversion parameter sensitivity analysis is also provided on the optimal operation of the smart energy distribution company. The proposed model is thoroughly tested on a 15-bus distribution grid system, and the numerical results prove the effectiveness of the model in risk management.
RNA Interference and Cancer

RNA interference (RNAi) is an evolutionally conserved gene silencing mechanism present in a variety of eukaryotic species. RNAi uses short double-stranded RNA (dsRNA) to trigger degradation or translation repression of homologous RNA targets in a sequence-specific manner. Over the past decade RNA interference (RNAi) has emerged as a natural mechanism for silencing gene expression. This system can be induced effectively in vitro and in vivo by direct application of small interfering RNAs (siRNAs), or by expression of short hairpin RNA (shRNA) with non-viral and viral vectors. This review briefly describes the molecular principles underlying of RNAi phenomenon and discuss the main technical issues regarding optimization of RNAi experimental design. Keywords: RNA interference (RNAi), double strand RNA (dsRNA), short hairpin RNA (shRNA)
Residential real estate investments from the perspective of Act on Social Security Benefits

Slovenska populacija se pospeseno stara. z rastjo pricakova-ne življenjske dobe postaja delež socialnih transferjev v javnem proracunu nevzdržen. Da bi lahko družba se naprej vzdrževala vitalno skupnost starostnikov, ponosnih na svojo dolgoživost, je potrebno razviti ekonomske in družbene institucije za izboljsanje nastalega stanja v družbi, ki je posledica njenega staranja. v Evrop-ski uniji so države clanice odgovorne za planiranje, financiranje in administracijo sistemov socialne varnosti za starajoce se prebival-stvo. Evropska unija podpira države clanice pri pripravi reform in priporoca paralelno zasledovanje treh dolgorocnih ciljev (a) do-stopnost do kvalitetne zdravstvene oskrbe in socialnih storitev (b) izboljsanje kvalitete dolgotrajne oskrbe (c) zagotovitev vzdržnosti financiranja teh storitev. v tem kontekstu je financiranje starajoce se populacije kljucno vprasanje. Slovenska vlada se je zaradi narascajocih stroskov zagotavljanja socialne varnosti odlocila, da samo starostnikom, ki niso lastniki nepremicnin in nimajo zadostnih sredstev za financiranje osnov-nih življenjskih stroskov, pripada nepovratna socialna pomoc.Ta zakon doloca, da so socialno varstveni prejemki oblika posojila lastnikom nepremicnin, pri cemer ima država pravico, da se ob izdaji odlocbe vpise v zemljisko knjigo kot hipotekarni upnik in vanjo zabeleži prepoved odtujitve nepremicnine. v tem clanku bomo predstavili nepravicnost zakona do lastnikov nepremicnin, saj socialno varstveni prejemki niso aktuarsko posteno odmerjeni, ker ne upostevajo vrednosti nepremicnine in starosti prejemnika. zakon o socialno varstvenih prejemkih zmanjsuje mobilnost pre-jemnikov in izpostavlja premoženje prejemnikov tveganju padca vrednosti zaradi prisilne prodaje na nelikvidnem slovenskem trgu nepremicnin ob smrti upravicenca. v clanku bomo pokazali, kako uvedba obratne hipoteke kot financnega instrumenta lahko v slo-venskem pravnem sistemu izboljsa socialno varnost starajocega se prebivalstva.
The influence of food particle size on gastric emptying and growth rates of fingerling African catfish, Clarias gariepinus Burchell, 1822

This study investigates the effect of feed particle size on gastric evacuation and growth of fingerling African catfish, Clarias gariepinus. Fish (0.97 ± 0.07 g) held in 40-cm diameter tanks at a stocking density of 5 fish L–1 (25 fish per tank) were presented with one of four sizes of food particles (1, 1.5, 2 and 3 mm diameter). Gastric evacuation rate could be described by an exponential function with small feed particles being evacuated more rapidly: values of 0.076, 0.054, 0.047 and 0.029 were observed for fish fed 1 mm, 1.5 mm, 2 mm and 3 mm food particles, respectively. Growth rates were highest for fish fed the 1.5 and 2 mm pellets. Based on these findings, recommendations are made on the optimum food particle size for C. gariepinus fingerlings.
The Potential Role of Nutraceuticals in Inflammation and Oxidative Stress

Nutraceuticals are defined as a food or food ingredients that prevent and treat diseases. They contain dietary supplements like proteins, vitamins and minerals, compound derived from natural sources. They have functions about delaying, preventing and treating chronic inflammatory diseases due to the presence of the phytochemicals. They have anti-inflammatory effects by inhibiting of the activation of NF- κ B, blocking the overexpression of tumor necrosis factor and interleukin-1, downregulation of the overexpression of cell adhesion molecules and inhibiting phospholipase A2, COX-2, lipoxygenease, iNOS, myeloperoxidase and inhibiting reactive oxygen species (ROS) generating enzyme activity and increasing ability to scavenge ROS. They have antioxidative role that can reduce the level of ROS and free radicals. They have effects on the process of lipid oxidation that inhibit or slow the formation of free alky radicals and cut off the free radical chain reactions.