Miracle-dz/UniFAR-FTUs huggingface dataset:
Concomitant Venous Thromboembolism [VTE] with Cancer and its Effect on Survival in Indian Patients

Cancer patients concurrently diagnosed with deep venous thrombosis have a poorer survival. In summary, Concurrent deep venous thrombosis confers a worse prognosis upon cancer patients. Stage at diagnosis, treatment effects, Medical comorbidities could explain differences by cancer site and time frame between deep venous thrombosis diagnosis and cancer outcome and overall survival. Abbreviations: VTE: Venous Thromboembolism; LMWH: Low-Molecular-Weight Heparin; UFH: Unfractionated Heparin; VKA: Vitamin K Antagonists Biomedical Journal of Scientific & Technical Research Cite this article: G Lohith, Radheshyam Naik, Shekar Patil, Niti Narang Raizada, Amrit Ram et al. Concomitant Venous Thromboembolism [VTE] with Cancer and its Effect on Survival in Indian Patients. Biomed J Sci&Tech Res 10(4)-2018. BJSTR. MS.ID. 001980. DOI: 10.26717/ BJSTR.2018. 10.001980. 2/3 Volume 10Issue 4: 2018 thrombosis, lower limb thrombosis and if prior prophylaxis were received was also noted. The date of diagnosis of VTE and last date of follow-up or up to death were considered for evaluation of overall survival. An exemption from institutional review board [Ethics and scientific committee] was obtained because these data were analyzed without the use of personal identifiers. Since no patients were contacted in this study, informed patient consent was not required. Statistical Methods: In the current study, proportions of venous thromboembolic episodes were evaluated and the mean overall survival was assessed using Kaplan Meier survival graphs. Results We identified 96 patients with diagnosis of 14 common cancers ranging from head and neck cancers to melanomas of the lower limb with concomitant diagnosis of DVT. Upper limb and lower limb DVTs were seen in 17 patients [17.71%] and 76 patients [79.71%] of patients respectively. No significant co-relation was noted to Venous thromboembolic episodes to Age, Sex, Medical co-morbidities, habits [Smoking, tobacco chewing and alcohol intake] and the diagnosis but there was a significant trend showing that DVT was prominent in advanced stages and in metastatic disease [P<0.05] and patients who have undergone chemotherapy, surgery and radiation had an higher risk of both upper and lower limb venous thromboembolic episodes ,though not significant statistically[P<0.03]. Early and prompt primary prophylaxis post diagnosis of DVTs proved to improve survival in the above study. Out of the total 96 patients enrolled into the study, 24 events [Deaths] were observed with a mean survival of 4.5±0.29 years (Tables 1& 2) and (Figure 1).
Análise in silico de microRNA com perfis de expressão alterados no modelo animal de autismo induzido por exposição pré-natal ao ácido valpróico

Background Autism spectrum disorders (ASD) are a highly heterogeneous disorder with unknown etiology. Some environmental factors could be related to the onset of autism, including exposure to valproic acid (VPA). MicroRNA are approximately 19–25 nucleotides noncoding RNA that act as post-transcriptional regulators of gene expression in several cellular processes. Dysregulation of miRNA expression might be related to some features of autism. Using quantitative polymerase chain reaction (PCR), we compared the expression miRNA from blood samples in an animal model of autism induced by VPA with control group and assessed the potential roles of these small RNA by correlating these modifications with putative altered levels of ASD-related target genes and signaling pathways.
Cholinergic Changes in the Hippocampus of Stroke-Prone Spontaneously Hypertensive Rats

Background and Purpose We investigated age-related changes in the central cholinergic systems in stroke-prone spontaneously hypertensive rats (SHRSP) to examine whether the regional and progressive cholinergic changes occur and are correlated with behavioral changes in the passive avoidance task. Methods Tissue levels of choline (Ch) and acetylcholine (ACh) were determined in the cerebral regions, including the hippocampus, of SHRSP (at two ages: 15 to 20 and 30 to 40 weeks) that had been tested in a passive avoidance task and were compared with those of age-matched controls, Wistar-Kyoto rats (WKY). With the use of in vivo microdialysis, high K+-stimulated release of hippocampal ACh, a functional parameter of the cholinergic system, was also determined in 15- to 20-week-old SHRSP. Results We found that 15- to 20-week-old SHRSP demonstrated a markedly lower level of hippocampal Ch than age-matched WKY. The decrease in the Ch level in 15- to 20-week-old SHRSP was observed in all regions examined; however, .
Transformación del hongo fitopatógeno "Sclerotium cepivorum" Berk empleando fusión de protoplastos = Transformation of the phytopathogenic fungus "Sclerotium cepivorum" Berk using protoplasts fusion

espanolLa pudricion blanca, una de las enfermedades mas preocupantes para los productores de ajo (Allium sativum), es causada por el hongo fitopatogeno Sclerotium cepivorum Berk. Con el fin de desarrollar procedimientos de biologia molecular para el hongo, en este trabajo se describe un metodo de transformacion empleando fusion de protoplastos en presencia de polietilenglicol 3350 (PEG). Se empleo el plasmido pAN7-1 que confiere resistencia a higromicina B, el cual contiene el gen higromicina fosfotransferasa B (hph) de Escherichia coli bajo el promotor gliceraldehido fosfato deshidrogenasa (gpdA) y el terminador antranilato sintetasa (trpC) del hongo filamentoso Aspergillus nidulans. Las transformantes recuperadas en el medio de cultivo de regeneracion papa dextrosa agar-sorbitol (PDA-S) en presencia de 200 ? gmL-1 de higromicina B se analizaron amplificando un fragmento del gen bacteriano hph mediante PCR con oligonucleotidos especificos: Hig 1, Sentido 5' GAC CTG CTG AGG TCC CTC 3' y Hig 2, antisentido 5' GCC CTC GGA CGA GTG CTG 3'. Se obtuvieron frecuencias de 12-18 transformantes de S. cepivorum por ? g de ADN vector. Los resultados descritos en el presente trabajo contribuiran en la caracterizacion molecular de genes involucrados en el fenomeno de patogenesis de S. cepivorum sobre el ajo. EnglishWhite rot, a disease of most concern to the producers of garlic (Allium sativum), is caused by the phytopathogenic fungus Sclerotium cepivorum Berk. In order to develop procedures of molecular biology to the fungus, in this paper we describe a transformation method using fusion of protoplasts in the presence of polyethylene glycol 3350 (PEG). We used the plasmid pAN7-1 which confers resistance to hygromycin B, which contains the hygromycin phosphotransferase gene B (hph) from Escherichia coli under the glyceraldehyde phosphate dehydrogenase promoter (GPDA) and the anthranilate synthase terminator (trpC) of the filamentous fungus Aspergillus nidulans. The recovered transformants in the regeneration medium potato dextrose agar-sorbitol (PDA-S) in the presence of 200 ? gmL -1 of hygromycin B were analyzed by amplifying a fragment of the bacterial gene hph by PCR with specific oligonucleotides: Hig 1, Sentido 5' GAC CTG CTG AGG TCC CTC 3' and Hig 2, antisentido 5' GCC CTC GGA CGA GTG CTG 3'. Obtaining 12-18 frequencies of transformants of S. cepivorum per ? g of vector DNA. The results described in this paper will contribute to the molecular characterization of the genes involved in the phenomenon of pathogenesis of S. cepivorum on garlic.
Spouses of First-Ever Stroke Patients

Background and Purpose—A stroke affects not only the patient but also the spouse. To better support the spouse during the acute phase of stroke, knowledge is needed about his or her experience with the situation. The aim of the present study was to study the well-being of the spouses of stroke patients during the acute state of stroke and to identify factors that may influence their well-being. Methods—Eighty-three consecutively enrolled spouses of first-ever stroke patients <75 years old participated. Their psychological well-being, measured by the Psychological General Well-Being Index 10 days after the stroke, was compared with norm values. Multiple analyses of correlation were performed to investigate the effects on psychological well-being of (1) age and sex, (2) level of impairment of the stroke patient, and (3) intrapersonal variables such as previous life satisfaction and view of the future. Results—The study group showed significantly lower psychological well-being compared with norm values excep.
Surgical therapy of medullary thyroid cancer and our clinical experiences

Aim: Medullary thyroid cancer (MTC) is a rare neuroendocrine tumor that originates from the thyroid parafollicular C cells and produces calcitonin. It is a quite aggressive disease with a potential to cause serious morbidity and mortality. In this study we aimed to report treatment outcomes of MTC, which has a bad prognosis and is difficult to manage. Material and Methods: The medical records of 1287 patients who were operated on for thyroid cancer between 2009 and 2018 were retrospectively assessed. Twenty-one patients (1.6%) were diagnosed with MTC. Results: Eleven (52.4%) patients were females. The age range of the patients was 54(14-85) years. Sixteen (76.2%) cases were sporadic and 5 (23.8%) were familial. Twelve patients underwent bilateral total thyroidectomy + central and unilateral neck dissection, 5(23.8%) bilateral total thyroidectomy + central and bilateral neck dissection, 4(19%) bilateral total thyroidectomy. Pathology examination revealed lymph node metastasis in 13(61.9%) patients. Three (14%) patients had simultaneous papillary thyroid cancer. Mean duration of follow-up was 52(3-96) months. Five (23.8%) patients suffered recurrence cervical lymph nodes (6 months later), lungs and bone metastasis (at 12th and 18th months), lungs (at 12thmonth), mediastinal lymph nodes (at 15th months), liver metastasis (at 6th months). Seven (33%) patients underwent chemo-radiotherapy. Conclusion: Surgery is the gold standard to control loco-regional disease and the only curative method among the available therapies in MTC treatment. Despite having a low incidence, MTC may still lead to serious mortality and morbidity in delayed cases and/or when loco-regional control cannot be achieved.
Evaluation of the interventricular systolic relationship in patients with right ventricular apical pacing with ventricular inhibited pacing mode by echocardiography using mitral and tricuspid annular plane systolic excursions.

The right (RV) and left (LV) ventricles differ markedly in shape and myocardial contractile element density. Despite these differences, the synchronous electrical excitation and mechanical contraction of both the ventricles and the ventricular interdependence maintains the biventricular function in balance 13. This is known as ventricular synchrony. Certain disease states like ischemic heart disease (IHD), acute pulmonary embolism, conduction disorders like left bundle branch block (LBBB), preexcitation, and ventricular pacing alters this balance resulting in ventricular dyssynchrony. RV apical permanent pacemaker implantation (PPI) with ventricular inhibited pacing (VVI) mode is known to produce ventricular dyssynchrony by abnormal electrical and mechanical activation of the ventricles. Long-term RV apical pacing is also associated with detrimental effects on cardiac structure and left ventricular (LV) function15. Echocardiographic evaluation of LV and RV systolic function by measuring mitral annular plane systolic excursion (MAPSE) 8 and tricuspid annular plane systolic excursion (TAPSE)9 and their ratio (MAPSE/TAPSE ratio)12 has been shown to correlate with ventricular synchrony in normal healthy individuals. M-mode echocardiographic evaluation of MAPSE, TAPSE, and their ratio can provide information regarding the degree of ventricular dyssynchrony in patients with VVI pacing. AIMS AND OBJECTIVES: 1. To assess the systolic function of left and right ventricle by mitral and tricuspid annular plane systolic excursions in patients with VVI pacing. 2. To analyse the interventricular systolic relationship using MAPSE/TAPSE ratio in patients with VVI pacemaker implantation. CONCLUSIONS: 1. Chronic VVI pacing from RV apex is associated with varying degrees of LV and RV dysfunction. 2. The degree of prolongation of the paced QRS duration is a powerful determinant of worsening ventricular function. 3. Advanced age is associated with both a marked prolongation of the paced QRS duration and progressive ventricular dysfunction in patients with VVI pacing. 4. MAPSE and TAPSE are useful parameters for quickly assessing LV and RV functions in VVI pacing. 5. LVEF and MAPSE/TAPSE ratio have linear correlation for LV dysfunction in VVI pacing. 6. MAPSE/TAPSE ratio is a novel indicator of ventricular dysfunction and lower levels are suggestive of associated interventricular dyssynchrony. However, this ratio needs further validation by larger randomized studies.
The crucial roles played by HNF1β during kidney development

HNF1β is a transcription factor expressed during nephrogenesis in the ureteric bud and in the tubular epithelia derived from the metanephric mesenchyme. Mutations in HNF1B/MODY5 represent one of the most prevalent human genetic defects responsible for Congenital Abnormalities of Kidney and Urinary Tract (CAKUT). To investigate the role played by HNF1beta during kidney development, I made use of a CRE-LoxP strategy to inactivate Hnf1b in different compartments and at different time points during mouse kidney morphogenesis. My results showed that Hnf1b is required for the expression of several key genes including Wnt9b, Emx2, Pax2 and Lhx. These genes are normally expressed in the ureteric bud and their absence leads to the formation of rudimentary kidneys, characterized by abnormal ureteric bud branching and lack of mesenchymal to epithelial transition. On the other hand, the specific inactivation of Hnf1b in the metanephric mesenchyme does not affect the early steps of epithelisation but leads to severe malformation of nephron precursors. In the absence of HNF1β, S-Shaped bodies lack the formation of a bulge of epithelial cells that normally give rise to Henle’s loops and proximal tubules. At the molecular level, these defects are associated with a decreased expression of Dll1, a Notch ligand that is directly controlled by HNF1β. This downregulation leads to a defective activation of the Notch pathway in the prospective tubular compartment of comma and S-shaped bodies. Preliminary results using a highly chimeric inactivation of Hnf1b in the mesenchyme showed that the spotted absence of Hnf1b does not prevent nephrogenesis. However, tubules are shorter and partially cystic. My results showed that murine models for Hnf1b deficiency recapitulates many of the malformations described in MODY5 patients. All together, my results improved our understanding of the genetic program controlled by HNF1β during kidney development whose dysfunction may lead to CAKUT in patients
Effects of Saccharomyces cerevisiae, Thepax and their combination on blood enzymes and performance of Japanese quails (Coturnix japonica).

The present study was conducted to investigate the influence of probiotic, prebiotic and their combination as synbiotic on broiler performance, organs weight and blood enzymes of Japanese Quails. Total of 192 one-day old Japanese quail chicks were randomly assigned to 4 treatments and 4 replicates. The experimental diets consisted of a basal diet (A) without additive (Control), (B) 2 g/kg Saccharomyces cerevisiae(probiotic), (C) 1 g/kg Thepax (prebiotic) and (D) 1 g/kg Saccharomyces cerevisiae+ 0.5 g/kg Thepax (synbiotic) added to the basal diet. Birds fed probiotic and prebiotic showed elevation in body weight compared to other groups (P<0.05). Feed intake of birds fed probiotic and prebiotic were higher than control group (P<0.05). Birds fed on probiotic and prebiotic exhibited a better feed conversion ratio compared to synbiotic fed and control groups (P<0.05). Dressing percentage of birds fed probiotic (74.9±2), prebiotic (76.2±3) and synbiotic (73.7±1) were more than control group (70.1±2) (P<0.05). The both male and female birds fed additives showed decrease in liver weight (P<0.05). The relative weight of heart was decreased in the males fed pr obiotic and prebiotic compared to synbiotic fed and control groups (P<0.05). The females fed prebiotic showed decrease in heart weight (P<0.05). The activity of ALP in females depressed by prebiotic consumption (P<0.05) whereas in males it was lower in probiotic fed group (P<0.05). AST activity in males fed prebiotic increased (P<0.05). In both genders CPK activity was higher in prebiotic and synbiotic fe d groups (P<0.05). Results indicated using probiotic (Saccharomyces cerevisiae) has positive effects on performance and normal activity of enzymes. Prebiotic ( Thepax) has positive effects on performance and reduction weight of heart and liver in Japanese quails. Synbiotic (Saccharomyces cerevisiae + Thepax) has positive effects on reduction weight of liver in both genders of Japanese quails.
Arbuscular mycorrhizal fungi

The potential disease suppressiveness of arbuscular mycorrhizal (AM) fungi of various origins on Bipolaris sorokiniana in barley has been investigated. Firstly, a survey considering the occurrence of AM fungi in arable fields in Sweden were conducted with the aim to exploit site specific genetic resources in relation to disease suppressiveness. Arbuscular mycorrhizal fungi were present at all 45 sampling sites surveyed all over Sweden at densities ranging from 3 up to 44 spores per gram air dried soil. The highest spore density was found in a semi-natural grassland and the lowest were found in a cereal monoculture. The AM fungi were then multiplied in trap cultures in the greenhouse with the aim to use these for studying potential disease suppressiveness. Thus, the effects of the AM fungi trap cultures on the transmission of seed-borne B. sorokiniana in barley were investigated, using the trap culture inocula, but also including inocula consisting on spore mixtures. The arbuscular mycorrhizal fungi were able to suppress the transmission of B. sorokiniana in aerial parts of barley plants. The degree of suppression varied with the origin of the AM fungal trap cultures. The trap culture inoculum with the highest suppression of the B. sorokiniana transmission originated from an organically managed barley field with undersown ley. The two spore-inocula with the best suppression of the pathogen originated from fields with winter wheat and spring barley, respectively. Eventually, an in vitro method was developed for studying the effect of AM fungal colonisation of roots on the development of foliar diseases and the reaction of the actual host plant of the disease causing organism. Using the developed method, it was indicated that AM fungal colonisation of barley plant suppressed the development of leaf necroses due to B. sorokiniana. Further in vitro studies on the interaction between B. sorokiniana and arbuscular mycorrhizal fungi showed that B. sorokiniana decrease the germination of the AM fungal spores. In conclusion, AM fungi suppress the development of B. sorokiniana in barley. My data suggest that for biocontrol of B. sorokiniana AM fungi should be considered.
Association of Type 2 Diabetes With Cyclooxygenase-Mediated Inflammation and Oxidative Stress in an Elderly Population

Background— Involvement of cyclooxygenase (COX)-mediated inflammation in type 2 diabetes has not been studied, and the association between cytokine-mediated inflammation and diabetes is not fully clarified. Methods and Results— 15-Keto-dihydro-prostaglandin F2α (a metabolite of prostaglandin F2α and an indicator of COX-mediated inflammation), high-sensitivity C-reactive protein (CRP), serum amyloid protein A (SAA), 8-iso-PGF2α (a nonenzymatic, free radical product of arachidonic acid and an indicator of oxidative stress), and α-tocopherol were measured in a population-based sample of 77-year-old men (n=765), in which 112 men had type 2 diabetes. The inflammatory indicators were increased in men with diabetes (urinary 15-keto-dihydro-PGF2α, P<0.001, CRP and SAA, P<0.05). However, when adjusted for body mass index, waist circumference, or fasting insulin, no association was found between diabetes and CRP or SAA. The oxidative stress indicator 8-iso-PGF2α in urine was increased (P<0.01) in men with diabetes.
Comparing Mean Platelet Volume Values in Patients with Recurrent Aphthous Stomatitis and Patients with Behcet’s Disease

Recurrent oral aphthae (ROA) are among the most common diseases of the oral mucosa and 20% of the population is affected. It is an inflammatory condition whose etiology is not clearly known and appears in the oral mucosa as painful, recurrent, single or multiple ulcers (1, 2). It is thought to occur due to immunemediated epithelial damage as a result of the disruption of the immune system. The lesions usually appear as white membranes on the ground and as erythematous halo in the periphery. It is mostly seen in non-keratinized oral mucosa surfaces (3). There are 3 types as minor, major and herpetiform. While minor ulcers are less than 1 cm and heal without a scar, major aphthae are over 1 cm and the healing period can take weeks or months, and they heal by scarring. Herpetiform aphthae consist of a number of small minor aphthae in group (4).
Epstein-Barr virus and cellular DNA damage response

EBV is an oncogenic human gammaherpesvirus which infects B lymphocytes and epithelial cells. New virus replication occurs in both cell types and EBV encodes proteins to replicate its own DNA. Selective activation of the DNA damage response (DDR) is thought to occur in order for the virus to efficiently conserve its DNA sequence. Components of the double strand break homologous repair pathway are known to be recruited to viral replication compartments and loaded onto viral DNA to achieve efficient and faithful replication of the viral DNA. Using an epithelial cell line latently infected with EBV, we demonstrated interactions between the phosphorylated replication protein A (RPA) and the viral lytic proteins BALF2 (DNA binding protein) and BMRF1 (polymerase accessory factor). However, despite its presence at replication centres, we failed to identify its direct interaction with the viral proteins by co-immunoprecipitation and mass spectrometry. Analysis of viral gene expression after ATM inhibition suggests that ATM does not promote the expression EBV lytic genes and is perhaps inhibitory. However, we did identify a novel interaction between BALF2 and the mitochondrial H+-Ca2+ exchanger LETM1, suggesting a new role for BALF2 possibly in inhibition of apoptosis during the viral lytic cycle.
Long term complications of Polycystic ovary syndrome

Polycystic ovary syndrome (PCOS) is emerging as most common endocrine disorder in women during reproductive years with a prevalence ranging from 6 to 10%. 1,2 Clinically, it presents with ovulatory dysfunction, hyper androgenism and/or polycystic ovarian morphology with exclusion of other etiologies [1,2]. It is a heterogenous condition with multiple clinical phenol types widely depending on age, genotype, ethnicity, bodyweight, lifestyle and environmental factors differently affecting the early and long-term risks.
Role of Immunosensors for Improved Animal Health Management

*1Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities, GBPUA and T, Pantnagar, Uttarakhand, India 2Department of Mechanical Engineering, School of Automobile, Mechanical and Mechatronics Engineering, Manipal University, Jaipur, Rajasthan, India 3Department of Veterinary Pathology, College of Veterinary and Animal Sciences, GBPUA and T, Pantnagar, Uttarakhand, India