Miracle-dz/UniFAR-FTUs huggingface dataset:
β-sitosterol, a plant sterol, induces apoptosis and activates key caspases in MDA-MB-231 human breast cancer cells

The objective of the present study was to evaluate the effect of beta-sitosterol, a plant sterol that induces apoptosis in breast cancer cells, on two pathways leading to apoptosis. These pathways are classified based on the localization of the initiated signal, extrinsic and intrinsic pathways. Extrinsic and intrinsic pathways are catalyzed by caspases 8 and 9, respectively, which leads to the activation of the executioner caspase 3. The results of the present study indicate that beta-sitosterol supplementation at 16 microM for 3 days to MDA-MB-231 cells induces 39% and 80% increases in the activities of caspases 8 and 9, respectively, compared to cholesterol supplemented cells or controls. There was also a 3-fold increase in the activity of caspase 3. Sterol treatment had no effect on the quantities of the enzymes. It is concluded that beta-sitosterol may induce apoptosis through the two pathways but was more pronounced on the intrinsic pathway.
Using Microteaching to Improve Preservice Elementary Teachers' Physical Science Content Knowledge.

This study examined the impact of microteaching experiences of preservice elementary teachers enrolled in a science teaching methods course. The microteaching lessons targeted the physical science concepts represented in the Texas Educational Agency’s exam for early childhood through sixth grade teacher certification. The results of this study demonstrate that targeting preservice elementary teachers’ specific areas of weakness in content through microteaching, feedback, and reflection can have a positive impact on the science subject teacher certification exam passing rate. Additionally, this type of intervention provides preservice teacher programs with another technique to improve science content understanding.
Spectroscopic Approaches to the Understanding of Water in Foods

This chapter reviews NMR and dielectric studies of hydration. It concentrates mainly on protein hydration as a model for foodstuffs. The relevant areas of theory are discussed and illustrative results from the literature are given. It is concluded that the notion of “bound water,” in the context of food systems where there is an excess of water, is not useful one. The dynamic perturbation of water by biopolymers is very small and cannot be used to account for properties such as water holding. Effects of biopolymers on such features as the freezing of water and vapour pressure are entirely consistent with normal thermodynamic behaviour and need no special explanation. There is clear evidence that when proteins reach an approximate water-to-protein ratio of about 0.3 to 1, there is a transition in behaviour. This is reflected across very wide range of measurements, and it seems to be associated with a point where the effects of water on the internal protein dynamics cease.
Devenir des personnes vivant avec le VIH sous une association TRIO dans la cohorte Aquitaine ANRS CO3 : vers une stratégie de simplification thérapeutique ?

Introduction : notre etude s’interesse a l’evolution des personnes vivant avec le VIH (PVVIH) ayant beneficie d’une association par darunavir booste par le ritonavir, raltegravir et etravirine (TRIO) dans la cohorte Aquitaine ANRS CO3. La tendance actuelle est a l'allegement therapeutique. Or, dans les essais cliniques, les PVVIH avec un virus multiresistant ne sont pas inclus. En effet, si la strategie TRIO a ete une alternative therapeutique de choix chez les patients en echec virologique presentant un virus multiresistant, elle peut neanmoins presenter des inconvenients a long terme sur le risque cardiovasculaire, les interactions medicamenteuses et la degradation de la qualite de vie due a une prise importante de comprimes par jour. Methode : etude descriptive non interventionnelle evaluant de maniere retrospective l’evolution virologique et therapeutique des patients traites par une association TRIO dans la cohorte Aquitaine ANRS CO3 entre Fevrier 2007 et Septembre 2018. L’objectif principal etait d’evaluer la proportion de patients en echec virologique sous TRIO, definit par deux charges virales (CV) superieures a 50 copies/ml a partir du 6eme mois. Les objectifs secondaires etaient de decrire les caracteristiques des patients en echec sous l'association TRIO, d’etudier la reponse virologique tout au long du suivi, d'evaluer la mortalite et la proportion de patient ayant ete simplifie. Resultats : cent trente-six patients ont ete inclus. La CV etait superieure a 50 copies/mL chez 92 patients (67,6 %) et inferieure a 50 copies/mL chez 44 patients (32,4 %). Le genotype de resistance cumule a l'inclusion retrouvait une resistance a l'etravirine (ETR) chez 40,8 % des patients, une resistance au darunavir/ritonavir (DRV/r) dans 16 % des cas et une resistance au raltegravir (RAL) dans 1,4 % des cas. Vingt-neuf patients (30,2 %) ont presente un echec virologique avec des mutations de resistance principalement selectionnees sur le gene de integrase. La duree mediane du suivi etait de 7,8 ans (IC95%, 4-9,6) et 27 patients (19,9 %) sont decedes, dont 19 (13,8 %) sous l'association TRIO. Lors de la derniere visite, 9 patients (8,3 %) recevaient encore l'association TRIO. En revanche, 55 patients (40,4 %) etaient sous bitherapie et un patient (0,7 %) sous monotherapie de DRV/r. Parmi ces patients alleges, 96,2 % avaient une CV indetectable. Conclusion : cette etude nous a permis d’apprecier l’evolution virologique des personnes vivant avec le VIH ayant beneficier d’une association TRIO dans la cohorte Aquitaine ANRS CO3. Cette evolution a ete favorable et nous avons constates qu'une proportion importante de patients avait ete allege sur le plan therapeutique. Ces resultats doivent etre completer par une etude de plus grande ampleur afin d’etudier l’efficacite et la securite des strategies d’allegement chez les patients multiresistants.
Replacing maize with sweet potato in diets for chicks

1. Rhode Island Red chicks and chicks of the indigenous fowl of Nigeria were fed for ten weeks on diets of 227.5 g protein and 11.92 to 13.72 MJ ME/kg in which sweet potato was substituted for maize. 2. Replacing up to 344.3 g maize/kg with sweet potato did not significantly affect body weight, food intake, food conversion, nitrogen retention, mortality and relative weights of body parts at 10 weeks of age. 3. Whereas crude protein contents of liver, gizzard, heart, lung and breast were not significantly affected by the diets, fat contents were significantly decreased by the replacement of 344–3 g maize/kg with sweet potato.
Vanillin cell sensor

Our project for iGEM 2006 consisted of designing a cellular vanillin biosensor. We used an EnvZ -E. coli strain as a chassis, and constructed two different devices: a sensor and an actuator, assembled using OmpR-P as a standardised mediator. The sensor device contained a computation- ally designed vanillin receptor and a synthetic two-component signal transduction protein (Trz). The receptor protein was based on a ribose-binding protein as scaffold. The Trz was built by fusion of the periplasmic and transmembrane domains of a Trg protein with an EnvZ kinase domain. When the receptor complex binds Trg, an allosteric motion is propagated to the cyto- plasmic EnvZ kinase domain, resulting in autophosphorylation and subsequent phosphate transfer to the OmpR transcription factor, which finally induces transcription of the ompC promoter. As actuator, we used a synthetic transcriptional circuit, which implements an OmpR-P band detector having GFP and RFP as an output. We designed this circuit using a synthetic promoter working as an AND gate, which is synergistically activated by cI and CRP. Our constructed Trg-EnvZ fusion and AND promoter will be very useful to future synthetic biology projects. 1 Aims of the project The main goal of this project was to introduce students to synthetic biology through the iGEM competition (1), while trying to do an interesting project that could generate parts to be reused by many future projects in synthetic biology. We chose to design a cellular biosensor having vanillin as an input and the expression of two reporters as output. To perform this, we constructed two devices: a sensor (based on a receptor and a two-component signal transductor) and an actuator (which received the intermedi- ate signal and expressed the reporters). We used a phos- phorylation mechanism from EnvZ as transmembranal pathway using OmpR-P as a standardised mediator (Fig. 1). To avoid interference with the natural pathways and have a better control of the kinase/phosphatase domain, we chose an EnvZ - E. coli strain as a chassis. The receptor domain of the sensor was inspired on the work by the group of Dr Hellinga, in which a sensor of
Siklus Belajar PODS (Predict-Observe-Discuss-Synthesize)

Pembelajaran fisika yang cenderung dilakukan dengan cara guru memberikan berbagai informasi berupa fakta dan persamaan-persamaan matematis kepada siswa tidak memfasilitasi siswa untuk menemukan sendiri bagaimana hubungan-hubungan antara fakta-fakta yang ia peroleh. Hal ini tentu saja dapat mengakibatkan pembelajaran menjadi kurang menarik bagi siswa, pemahaman konsep yang kurang dan tingkat retensi siswa yang rendah. Dalam pembelajaran fisika, guru haruslah menghadirkan pembelajaran aktif. Salah satu pembelajaran aktif yang dapat digunakan dalam rangka meningkatkan pemahaman saintifik adalah siklus belajar PODS (Predict-Observe-Discuss-Synthesize) yang merupakan siklus pembelajaran sains yang menuntut siswa untuk terlibat aktif dalam pembelajaran Pembelajaran dengan siklus belajar PODS (Predict-Observe-Discuss-Synthesize) dapat meningkatkan pemahaman konsep siswa, dimana siswa dapat lebih aktif dalam mengkonstruk sendiri pemahamanya, guru tidak lagi menjadi sumber pengetahuan melatihkan siswa untuk dapat membangun pemahaman tersebut.
Functional and harmonic analysis of noncommutative Lp spaces associated to compact quantum groups

This thesis is devoted to studying the analysis on compact quantum groups. It consists of two parts. First part presents the classification of invariant quantum Markov semigroups on these quantum homogeneous spaces. The generators of these semigroups are viewed as Laplace operators on these spaces.The classical sphere, the free sphere, and the half-liberated sphere are considered as examples and the generators of Markov semigroups on these spheres are classified. We compute spectral dimensions for the three families of spheres based on the asymptotic behavior of the eigenvalues of their Laplace operator.In the second part, we study of convergence of Fourier series for non-abelian groups and quantum groups. It is well-known that a number of approximation properties of groups can be interpreted as some summation methods and mean convergence of associated noncommutative Fourier series. We establish a general criterion of maximal inequalities for approximative identities of noncommutative Fourier multipliers. As a result, we prove that for any countable discrete amenable group, there exists a sequence of finitely supported positive definite functions, so that the associated Fourier multipliers on noncommutative Lp-spaces satisfy the pointwise convergence. Our results also apply to the almost everywhere convergence of Fourier series of Lp-functions on non-abelian compact groups. On the other hand, we obtain the dimension free bounds of noncommutative Hardy-Littlewood maximal inequalities in the operator-valued Lp space associated with convex bodies.
Gelatin: The Paramount Food Additive

Abstract Of all the hydrocolloids in use today, surely none has proven as popular with the general public and found favor in as wide a range of food products as gelatin. A sparkling, clear dessert jelly has become the archetypal gel and the clean melt‐in‐the‐mouth texture is a characteristic that has yet to be duplicated by any polysaccharide. Despite its apparently unfashionable status, more gelatin is sold to the food industry than any other gelling agent. It is relatively cheap to produce in quantity, and there is a ready supply of suitable raw material. The traditional sources of gelatin include bovine and pig skins and demineralized bones and hooves. However, recent studies have shown that there are viable new sources of gelatin such as marine fish skins and bones. Researchers have further sought to develop gelatin derivatives or modified gelatins like coldwater soluble gelatin, hydrolyzed gelatin and esterified gelatin.
Study of the Quality of Website Information Related to Promotion of Pathology

Aim: The aim of this study is to evaluate the reliability of information about pathology available to people at Turkish websites. Material and Method: The terms “pathology,” “what is pathology,” and “importance of pathology” were scanned into the most commonly used search engine in Turkey of “Google” in Turkish at 2018. The terms used in the research were considered to be terms used by patients and the public. The first 30 results for each search were used. The same websites reached as a result of the search were eliminated. Each website was classified according to the author as health-related organizations, news, personal, doctors, and undetermined. The websites were assessed according to 15-question scale that was prepared to assess information related to definition, functions, and importance of pathology. Results: The search reached 90 websites. Of these, nine were the same website. As a result, the evaluation included 81 websites. The mean points for the websites were 1.432 (min: 0, max: 13, and standard deviation: 4.28). When the information is distributed according to topic headings, proportionally, the most sufficient information appeared to be about “what is pathology” at 32.1%. The most insufficient information was given about “how does a pathology laboratory work (2.5%),” “place of pathology in genetic studies (2.5%),” and “importance of preventive medicine (2.5%). ” Conclusion: According to our study, the information on “what is pathology” and “important of pathology” on the internet is not sufficient. Pathologists should be aware of this deficiency, and people should be properly informed about the importance of pathology.
Neisseria SPECIES ISOLATED FROM DOLPHINS1

Neisseria species have been identified as part of the normal microbial flora of several domestic and experimental animals,’ but they have not however, been observed in marine mammals.3 We are presently studying on a long-term basis, the total microbial profile (bacteria, viruses, and fungi) of marine mammals in their natural habitat and the interand intra-species changes which occur when the animals are brought into contact with man, other animals, and when placed in isolation under husbandry conditions.
Sampling methods for rumen microbial counts by Real-Time PCR techniques

Abstract Fresh rumen samples were withdrawn from 4 cannulated buffalo females fed a fibrous diets in order to quantify bacteria concentration in the rumen by Real-Time PCR techniques. To obtain DNA of a good quality from whole rumen fluid, eight (M1-M8) different pre-filtration methods (cheese cloths, glass-fibre and nylon filter) in combination with various centrifugation speeds (1000, 5000 and 14,000 rpm) were tested. Genomic DNA extraction was performed either on fresh or frozen samples (-20°C). The quantitative bacteria analysis was realized according to Real-Time PCR procedure for Butyrivibrio fibrisolvens reported in literature. M5 resulted the best sampling procedure allowing to obtain a suitable genomic DNA. No differences were revealed between fresh and frozen samples.
Molecular prediction of rat liver triglycerides by high performance liquid chromatography

Abstract Triglyceride composition of rat liver was analyzed by HPLC with a non-linear elution gradient of acetone in acetonitrile using a light-scattering detector. Triglyceride molecular species were predicted by means of simple and multiple linear regression analysis between the log k' of the HPLC peaks and molecular variables, equivalent carbon number (ECN) of the possible triglycerides, and chain length (CL) and number of double bounds (DB) of each of the fatty acids in the glycerol molecule. The triglycerides presenting high random percentages were considered the principal molecular species in each HPLC peak. The main molecular species of rat liver triglycerides were palmitoyl-oleoyl-linoleoyl-glycerol, triolein, palmitoyl-dilinoleoyl-glycerol, dipalmitoyl-oleoyl-glycerol and oleoyl-dilinoleoyl-glycerol. The systematics predicted molecular species with arachidonic acid, such as dioleoyl-arachidonoyl-glycerol, dipalmitoyl-arachidonoyl-glycerol, and oleoyl-linoleoyl-arachidonoyl-glycerol, and with doco.
Commercial oyster stocks as a potential source of larvae in the regeneration of Ostrea edulis in Strangford Lough, Northern Ireland

Ostrea edulis was extremely rare in the wild in Strangford Lough from the early 1900s until renewed spatfall was observed at a number of sites in the 1990s. A monitoring programme was undertaken to investigate the presence and distribution of planktonic oyster larvae at nine sites around the lough between June and September in 1997 and 1998 as a precursor to studies of spatfall patterns. Larval densities at sites in the northern basin of the lough were significantly higher than those in the southern basin where larvae were lacking or in low numbers. Densities and sizes of oyster larvae showed significant temporal variation suggesting pulsed larval release. Larval densities also showed significant spatial variation with higher densities at sites closer to commercial stocks pointing to these as the main source of oyster larvae. This hypothesis was supported during a larval flux study over a complete tidal cycle which indicated a 90% net tidal movement of O. edulis larvae from the entrance of the bay where commercial stocks were held to the main body of the lough. Thus the maintenance of dense commercial stocks of flat oysters may provide the key to the redevelopment of native oyster beds in Strangford Lough and elsewhere by providing an initial broodstock nucleus from which larvae can be exported.
Adaptations of birds for life in deserts with particular reference to Larks (ALAUDIDAE)

Birds inhabit all deserts, an unusual observation because they are mainly diurnal, non-fossorial, and have the highest mass-specific metabolism of all vertebrates. Many of the “adaptations” of birds for life in deserts are thought by some to be intrinsic in all birds, and thus have been considered to be pre-adaptations. Nevertheless, recent evidence suggests that desert birds do differ from birds of more mesic habitats, and physiological adaptations include reductions in basal metabolism and cutaneous water loss. Behavioural mechanisms that permit a desert existence include nomadism, enabling them to move away from low resource patches to more productive patches, and the ability to use features of their environment to escape from high temperatures. Desert birds can prevent overheating by using rodent burrows and other shelters during the heat of the day. Birds in deserts include a high proportion of ants in their diet, a resource not often exploited by birds of more temperate environments, and they also show seasonal or opportunistic shifts in their diet, feeding on seeds when available, and on more succulent green plant matter, such as leaf bases of grasses, after rain. Certain taxa, for example sandgrouse (Pteroclidae) and larks (Alaudidae), are mainly found in deserts. The adaptations in some species of larks are discussed in detail.
Evolution of larval food plant associations in Delias Hübner butterflies (Lepidoptera: Pieridae)

A review of larval food plants of the genus Delias is presented. Larvae specialize primarily on aerial‐stem and root hemiparasites (“mistletoes”) in the order Santalales. Although butterfly food plant associations have been recorded for only a small proportion of the genus (28 species or ∼11%, representing 12/24 species‐groups), available data suggest that the family Loranthaceae is used most frequently (77%), followed by the Santalaceae sensu stricto (14%) and Viscaceae (8%). With the possible exception of Euphorbiaceae (1%), almost all non‐mistletoe records are considered to be erroneous and, in most cases, probably represent the mistletoe host tree on which the larvae sometimes pupate. Of the eight major clades recognized in Delias, food plants have been recorded for six of these, although the majority of records (89%) are for three clades (hyparete, belladonna, nigrina). Optimization of the larval food plant data in the context of recent phylogenetic hypotheses for both butterflies and plants revealed little evidence of cospeciation at the higher systematic levels. The most parsimonious reconstruction was an origin of larval feeding on Loranthaceae, followed by at least six independent colonizations to Santalaceae + Viscaceae. In contrast to related pierids in the Aporiina associated with mistletoes in which further shifts from aerial‐stem mistletoes to distantly related plants (e.g. host trees parasitized by mistletoes) have facilitated differentiation at the generic level, there is no firm evidence to indicate that such secondary, monomorphic shifts have evolved in Delias. However, larvae of D. henningia (pasithoe group of belladonna clade) from Palawan and Luzon, the Philippines, appear to be polymorphic, feeding on both Loranthaceae and Euphorbiaceae.
Redo Percutaneous Mitral Valvuloplasty (Redo PMV) in Patients With Recurrent Mitral Valve Stenosis: Immediate and Early Outcomes

Background: Symptomatic recurrent mitral valve stenosis develops in some patients after Percutaneous Mitral Valvuloplasty (PMV). This study assessed the immediate and early outcomes of redo PMV in patients with recurrent mitral valve stenosis after prior PMV.Methods: Fifty-four patients (40 women and 14 men, mean age of 38±8.2 years) underwent a redo (second) PMV for symptomatic restenosis of mitral valve [with Mitral Valve Area (MVA) < 1.5 cm2]. Redo PMV was performed at 5.8±1.2 years after the initial PMV.Results: In this study, 48 hours after the procedure, there was a substantial increase in MVA by 2-dimensional Echocardiography (GE, Vivid 7) from 1.0±0.2 to 2.2±0.4 cm2 (p<0.001) and a decrease in mean left atrial pressure from 27 ± 5 to 15 ± 4 mmHg (p<0.001) and in mean transmitral valve gradient from 15±4 to 2±1 mmHg (p Conclusion: Redo PMV can be performed successfully in patients with recurrent mitral valve stenosis following previous percutaneous valvuloplasty.
The histology and cytology of the electric organ of the electric EEL (Electrophorus electricus, L.)

This paper concerns the structure of the electric organ of Electrophorus electricus, L. , investigated by several histological methods. While many of the observations are confirmatory of earlier German work, particularly that of Ballowitz (1897), some new findings are presented regarding the mitochondria and the rodlets or '' Stabchen" associated with the organ. Moreover, a description will be given and figures presented of the electric organ as observed by phase contrast microscopy. These observations provide a background for a further investigation of this unusual tissue with the electron microscope. In the discussion an attempt Will be made to correlate the present morphological observations with recent physiological and biochemical data on the electric organ.
Lyme disease: A tick-borne spirochetosis

A treponema-like spirochete was detected in and isolated from adult Ixodes dammini, the incriminated tick vector of Lyme disease. Causally related to the spirochetes may be long-lasting cutaneous lesions that appeared on New Zealand White rabbits 10 to 12 weeks after infected ticks fed on them. Samples of serum from patients with Lyme disease were shown by indirect immunofluorescence to contain antibodies to this agent. It is suggested that the newly discovered spirochete is involved in the etiology of Lyme disease.