Miracle-dz/UniFAR-FTUs huggingface dataset:
verexpression of miR-378 is frequent and may affect treatment outcomes in atients with acute myeloid leukemia

MicroRNA miR-378 plays important roles in tumorigenesis by enhancing cell survival, reducing apoptosis, promoting tumor growth, angiogenesis and promoting cell migration and invasion. Abnormal expression of miR-378 has been observed in various types of cancers. The aim of this study was to investigate the expression status of miR-378 and its clinical significance in patients with acute myeloid leukemia (AML) using real-time quantitative PCR. miR-378 overexpression was identified in 26 of 84 (31%) AML patients. The patients with miR-378 overexpression had lower hemoglobin level than those without miR-378 overexpression (66 versus 78 g/L, respectively, P = 0.010). The frequency of miR-378 overexpression in iR-378 cute myeloid leukemia FAB-M2 subtype was higher than other subtypes (44% versus 20%, P = 0.032). Moreover, the frequency of miR-378 overexpression was higher in patients with t(8;21) than in others (64% versus 24%, P = 0.012). The status of miR-378 expression was not correlated with the mutations of eight genes (FLT3-ITD, NPM1, C-KIT, IDH1/IDH2, DNMT3A, C/EBPA and U2AF1). The difference in relapse-free survival was observed between patients with and without miR-378 overexpression (P = 0.049). These findings suggest that miR378 up-regulation is a common event and might have an adverse impact on prognosis in AML.
Roles of metabotropic glutamate receptors in glial function and glial‐neuronal communication

The amino acid glutamate plays a key role in brain function. One of the major roles of glutamate is to mediate fast excitatory neurotransmission via activation of ionotropic glutamate receptors (iGluRs). More recently, however, it has become clear that glutamate also serves a regulatory function through activation of receptors coupled to modulation of second messenger systems [metabotropic glutamate receptors (mGluRs)]. A body of evidence suggests that mGluRs regulate neuronal function through modulation of ion channels and enzymes to modulate cellular excitability and synaptic transmission. Interestingly, it has become clear that in addition to activation of neuronal receptors, glutamate can activate both iGluRs and mGluRs on glia. A growing body of evidence suggests that the mGluRs on glia play important roles in both glial function and mediation of intercellular signaling. © 1996 Wiley‐Liss, Inc.
Liver biopsy in evaluation of complications following liver transplantation.

AIM To analyze the role of liver biopsies in differential diagnosis after liver transplantation. METHODS A total of 50 biopsies from 27 patients with liver dysfunction out of 52 liver transplantation cases were included. Biopsies were obtained 0-330 d after operation, in which, 44 were fine needle biopsies, another 6 were wedge biopsies during surgery. All tissues were stained with haemotoxylin-eosin. Histochemical or immunohistochemical stain was done. RESULTS The rate of acute rejection in detected cases and total transplantation cases was 48.2% and 25.0%, chronic rejection rate in detected cases and total transplantation cases was 14.8% and 7.7%, preservation-reperfusion injury in detected cases and total transplantation cases was 25.9% and 13.5%, hepatic artery thrombosis rate in detected cases and total transplantation cases was 11.1% and 5.8%, intrahepatic biliary injury rate in detected cases and total transplantation cases was 7.4 % and 3.8%, CMV infection rate in detected cases and total transplantation cases was 3.7% and 1.9%, hepatitis B recurrence rate in detected cases and total transplantation cases was 3.7% and 1.9%, the ratio of suspicious drug-induced hepatic injury in detected cases and total transplantation cases was 11.1% and 5.8%. CONCLUSION Acute rejection and preservation-reperfusion injury are the major factors in early liver dysfunction after liver transplantation. Hepatic artery thrombosis and prolonged cold preservation may result in intrahepatic biliary injury. Acute rejection and viral infection may involve in the pathogenesis of chronic rejection. Since there are no specific lesions in drug-induced hepatic injury, the diagnosis must closely combine clinical history and rule out other possible complications.
Fortalecimiento de la relación universidad con las empresas como una necesidad para la inserción laboral de los estudiantes: Experiencias en la FIME

Entre los aspectos claves del exito en una empresa se encuentra el recurso humano. La universidad como principal generador de recursos humanos, debe considerar para la formacion de tales recursos las necesidades de la industria asi como tambien los medios que propicien su rapida insercion laboral. En este trabajo se exponen opiniones de la industria hacia los egresados y el entorno en relacion a los medios que favorecen la insercion laboral en los estudiantes de la Facultad de Ingenieria Mecanica y Electrica de la Universidad Autonoma de Nuevo Leon.
Concerns on “Dissection of Regulatory Elements During Direct Conversion of Somatic Cells Into Neurons” Paper

Direct Conversion of Somatic Cells Into Neurons” Paper Moein Yaqubi,* Abdulshakour Mohammadnia ,** and Ping Wee Department of Psychiatry, Sackler Program for Epigenetics and Psychobiology at McGill University, Ludmer Centre for Neuroinformatics and Mental Health, Douglas Mental Health University Institute, McGill University, Montreal, Quebec, Canada Department of Human Genetics, Faculty of Medicine, McGill University, Montreal, Quebec, Canada Department of Medical Genetics and Signal Transduction Research Group, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada
A VIRTUAL REALITY ENVIRONMENT FOR TESTING SOMATOSENSORY NEURO-PROSTHESES

INTRODUCTION The sense of touch plays a crucial role in our everyday lives. Touch allows us fine motor control, adds to our sense of self, and helps with our perception of the world around us [3]. Without it, accomplishing simple everyday tasks would become overwhelmingly difficult. Such is the life of approximately two million amputees in the United States today [2]. In order to improve the lives of amputees, advancements have been made allowing the prosthetics to interface with the subject’s brain. This neural link allows the subject to move the prosthetic device through the sheer power of their mind [3]. As a result the prosthetic begins to feel more natural to the amputee. But without sensory feedback, the subject still experiences difficulties in fine motor control and pain which appears to emanate from the missing limb [3]. During recent experiments at the Human Rehabilitation and Neural Engineering Laboratory (hRNEL), we have demonstrated it is possible to generate sensations which appear to emanate from a missing limb. In order to create these sensations, a subject must be implanted with an array of electrodes along their cervical spinal nerves. By electrically stimulating these electrodes, the subject begins to feel sensations originating from the location of their missing limb. Ideally if these sensations are incorporated with the prosthetic, the amputee’s will see improvements in fine motor control, and a reduction in pain. But these sensation are not ideal. In fact more often than not, they are accompanied by an unnatural buzzing feeling known as paresthesia [3]. Another issue is that the location of the produced sensations is not always exactly where it was intended to be. Combining both problems causes an unnatural feel which takes away from the benefits of sensory feedback restoration. Therefore in order to help amputees, we need to discover a way to improve the naturalness of the sensations generated through electrical stimulation.
Arf-like Protein 3 (ARL3) Regulates Protein Trafficking and Ciliogenesis in Mouse Photoreceptors*

Arf-like protein 3 (ARL3) is a ubiquitous small GTPase expressed in ciliated cells of plants and animals. Germline deletion of Arl3 in mice causes multiorgan ciliopathy reminiscent of Bardet-Biedl or Joubert syndromes. As photoreceptors are elegantly compartmentalized and have cilia, we probed the function of ARL3 (ADP-ribosylation factor (Arf)-like 3 protein) by generating rod photoreceptor-specific (prefix rod) and retina-specific (prefix ret) Arl3 deletions. In predegenerate rodArl3−/− mice, lipidated phototransduction proteins showed trafficking deficiencies, consistent with the role of ARL3 as a cargo displacement factor for lipid-binding proteins. By contrast, retArl3−/− rods and cones expressing Cre recombinase during embryonic development formed neither connecting cilia nor outer segments and degenerated rapidly. Absence of cilia infers participation of ARL3 in ciliogenesis and axoneme formation. Ciliogenesis was rescued, and degeneration was reversed in part by subretinal injection of adeno-associated virus particles expressing ARL3-EGFP. The conditional knock-out phenotypes permitted identification of two ARL3 functions, both in the GTP-bound form as follows: one as a regulator of intraflagellar transport participating in photoreceptor ciliogenesis and the other as a cargo displacement factor transporting lipidated protein to the outer segment. Surprisingly, a farnesylated inositol polyphosphate phosphatase only trafficked from the endoplasmic reticulum to the Golgi, thereby excluding it from a role in photoreceptor cilia physiology.
4:1 bandwidth digital five bit MMIC phase shifters

Five-bit monolithic microwave integrated circuits (MMIC) phase shifters that cover over two octaves of bandwidth are developed for functions such as electronic beamsteering and frequency translation. The authors describe two digitally controlled MMIC phase shifters optimized for different operating bandwidths. A lowband version of the phase shifter is designed for the 2.0-6.0-GHz frequency range, and a highband version is designed for the 4.5-18.0-GHz frequency range. Both versions maintain useful performance levels significantly beyond their design bandwidths. The typical measured circuit performance of a highband phase shifter chip in the 4.5-18-GHz band shows a maximum phase error of less than 20 degrees and a +or-1.15-dB maximum change in insertion loss with phase state. The measured circuit performance of a lowband phase shifter chip in the 2.0-6.0-GHz band shows a maximum phase error of less than 15 degrees and less than +or-0.6-dB insertion loss variation with phase state. <<ETX>>
UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL - UFRGS FACULDADE DE CIÊNCIAS ECONÔMICAS PROGRAMA DE PÓS-GRADUAÇÃO EM ECONOMIA CLUSTERS INDUSTRIAIS: VANTAGEM COMPETITIVA E DESENVOLVIMENTO REGIONAL

Technology transformation, both in computer science and telecommunications, has facilitated access to information, and also reduced access costs. This has caused the relation network among economical agents to become faster and have a wider geographical reach, narrowing the interaction between local and global. Thus, the organization on the search for insertion in the international market has focused on a regional process, where the cluster concept becomes a useful tool to answer different questions. These questions arise from the business cycle and company administration to the use of resources such as space, personnel, input and, most of all, knowledge dissemination. Industrial clusters remain idle in this new environment created by technological advancements, but they work as facilitators in the design of innovations that are introduced as positive external factors in generating regional economical development. They even encourage government participation, which becomes very active in some situations, seeking to promote and sustain the industrial clustering. The purpose of this paper is to examine the cluster, its determining role in obtaining competitive advantages in industry and its relation with regional development. Within this proposal, an analysis of the shoe industry cluster in Rio Grande do Sul Sinos Valley is also presented by means of the shift share analysis, with data supplied by the Annual Social Information Report (RAIS) from 1990 to 2001.
Changing the phospholipid composition of Staphylococcus aureus causes distinct changes in membrane proteome and membrane‐sensory regulators

The dynamic lipid composition of bacterial cytoplasmic membranes has a profound impact on vital bacterial fitness and susceptibility to membrane‐damaging agents, temperature, or osmotic stress. However, it has remained largely unknown how changes in lipid patterns affect the abundance and expression of membrane proteins. Using recently developed gel‐free proteomics technology, we explored the membrane proteome of the important human pathogen Staphylococcus aureus in the presence or absence of the cationic phospholipid lysyl‐phosphatidylglycerol (Lys‐PG). We were able to detect almost half of all theoretical integral membrane proteins and could reliably quantify more than 35% of them. It is worth noting that the deletion of the Lys‐PG synthase MprF did not lead to a massive alteration but a very distinct up‐ or down‐regulation of only 1.5 or 3.5% of the quantified proteins. Lys‐PG deficiency had no major impact on the abundance of lipid‐biosynthetic enzymes but significantly affected the amounts of the cell envelope stress‐sensing regulatory proteins such as SaeS and MsrR, and of the SaeS‐regulated proteins Sbi, Efb, and SaeP. These data indicate very critical interactions of membrane‐sensory proteins with phospholipids and they demonstrate the power of membrane proteomics for the characterization of bacterial physiology and pathogenicity.
Progesterone Receptor Loss Correlates with Human Epidermal Growth Factor Receptor 2 Overexpression in Estrogen Receptor–Positive Breast Cancer

Response to endocrine therapy in breast cancer correlates with estrogen receptor (ER) and progesterone receptor (PR) status. It was originally hypothesized that the ability of PR to predict response to endocrine therapy was due to the fact that PR is an estrogen-regulated gene and that its levels represented a marker of functional ER activity. However, it is now known that loss of PR can occur via multiple mechanisms, many of which do not include ER function, e.g., hypermethylation of the PR promoter and loss of heterozygosity of the PR gene. We have shown that growth factor signaling pathways can directly down-regulate PR levels via the phosphatidylinositol 3′-kinase (PI3K)/Akt/mTOR pathway, and that this can occur independent of ER. For example, overexpression of myr-Akt in MCF-7 cells causes complete loss of PR protein and mRNA but does not reduce ER levels or activity, thus generating ER+/PR− MCF-7 cells. Therefore, the absence of PR may not simply reflect a lack of ER activity but rather may reflect hyperactive cross-talk between ER and growth factor signaling pathways. Consistent with this hypothesis, several recent clinical studies have found that ER+/PR− breast cancers overexpress human epidermal growth factor receptor (HER) 1 and HER2 compared with ER+/PR+ breast cancers. Although HER receptors can lower ER levels, one study showed that loss of PR correlated with high HER2 levels in a multivariate analysis. Furthermore, loss of PTEN, a negative regulator of the PI3K/Akt signaling pathway, has been shown to be associated with specific loss of PR and no change in ER levels. Given the well-recognized resistance of ER+/PR− breast cancer to antiestrogens, more studies are needed to better understand the etiology of ER+/PR− breast cancer, particularly the analysis of other growth factor receptors and their downstream signaling intermediates with respect to PR status.
Summary of evidence pointing to a role of the dorsal cochlear nucleus in the etiology of tinnitus

Evidence has accumulated in the last decade that the dorsal cochlear nucleus (DCN) may be an important site in the etiology of tinnitus. This evidence comes from a combination of studies conducted in animals and humans. This paper will review the key findings, as follows. 1) Direct electrical stimulation of the DCN leads to changes in the loudness of tinnitus. This suggests that the loudness of tinnitus may be linked to changes in the level of neural activity in the DCN. 2) Exposure to tinnitus inducers, such as intense sound or cisplatin, causes neural activity in the DCN to become chronically elevated, a condition known as neuronal hyperactivity. 3) This hyperactivity is very similar to the activity that is evoked in the DCN by sound stimulation, suggesting that the hyperactivity represents a code that signals the presence of sound, even when there is no longer any sound stimulus. 4) Noise-induced hyperactivity in the DCN is correlated with tinnitus. Behavioral studies have demonstrated that animals exposed to the same intense sound that causes hyperactivity in the DCN develop tinnitus-like percepts. The correlation between the level of hyperactivity and the behavioral index of tinnitus was found to be statistically significant. 5) The DCN is a polysensory integration center, and electrophysiological studies have shown that both spontaneous activity and hyperactivity of neurons in the DCN can be modulated by stimulation of certain ipsilateral cranial nerves, such as the sensory branch of the trigeminal nerve. This ipsilateral modulation of DCN activity offers a plausible explanation of how tinnitus, when perceived on one side, can be modulated by certain manipulations of the head and neck on the side ipsilateral to the tinnitus, but rarely on the contralateral side. 6) The DCN exhibits various forms of neuronal plasticity that parallel the various forms of plasticity that characterize tinnitus. These findings collectively strengthen the view that the DCN may be a key structure that should be included as a target of anti-tinnitus treatment.
The major sigma factor (RpoD) from Helicobacter pylori and other gram-negative bacteria shows an enhanced rate of divergence

Sequence analysis of the Helicobacter pylori major sigma factor (RpoD) shows that it is highly divergent, which may be related to the marked diversity of the H. pylori chromosome. Furthermore, the rate of divergence of RpoD among other gram-negative bacteria is much greater than that among gram-positive bacteria. This suggests that RpoD from gram-negative bacteria is functionally less constrained than that from gram-positive bacteria.
Cold‐shock induction of a family of TIP1‐related proteins associated with the membrane in Saccharomyces cerevisiae

TIP1 is the first known cold‐shock‐and heat‐shock‐induced gene in Saccharomyces cerevisiae. Here it is demonstrated that a TIP1 homologue, TIR1, which had been previously cloned as SRP1 (serine‐rich protein), is strongly induced by a downshift in growth temperature from 30 to 10°C. We further cloned TIR2, which is transcribed at a low basal level but is increased strongly by cold shock and, to a lesser extent, by heat shock. The predicted protein sequence of TIR2 demonstrates remarkable homology to T1R1 (72.2%) and is also homologous with TIP1 (49%). TIP1, TIR1 and TIR2 are rich in both serine and alanine residues and each contains serine‐rich tandem repeats. The proteins contain putative N‐terminal signal peptides as well as hydro‐phobic C‐terminal sequences, indicating that the proteins may be membrane bound. The predicted protein sequences are also consistent with extensive O‐mannosylation as well as glycosyl‐phosphatidyl inositol (GPI) membrane anchoring. Cell fractionation analysis as well as studies using a yeast strain that is conditionally deficient in glycosylation demonstrate that TIP1 is a heavily modified membrane‐associated protein. Single, double combinations and triple mutants were created and none demonstrated any obvious phenotype, indicating that this family of genes is not essential for normal growth.
An Algebraic Approach to Quantum Systems Using Finite Group Representation

UNIVERSITY TEACHING Summer Instructor, Boston University • Sole instructor for 6-week intensive courses with typically 30 students. • Directed graduate student or undergraduate graders. • Courses taught: – MA113 Elementary Statistics I Summer 2015 – MA115 Statistics I Summer 2015 – MA123 Calculus I Summer 2016 – MA226 Differential Equations Summer 2017 Teaching Fellow, Boston University Sept. 2014 present • Courses taught: – EK102 Linear Algebra for Engineers – MA121 Calculus I for Social Sciences – MA122 Calculus II for Social Sciences – MA123 Calculus I – MA226 Differential Equations – MA411 Advanced Calculus – MA511 Analysis I – MA569 Operations Research
Attendance management system

In today's era regardless of the field of study or preference for defining data (quantitative, qualitative), accurate data collection is essential for maintaining the integrity of research. Selection of appropriate method and the device used for data acquisition reduces chances of errors occurring. Proposed system has a small handy hardware, a remote server and software components for acquisition of data manually or from sensors electronically. It could be used in doing survey's, closed loop control monitoring systems in industries, hospitals, attendance management system of schools and colleges etc. This paper presents a design and framework for taking attendance in schools and colleges, for making troublesome process of taking and compiling of attendance simple and efficient. As its targeted users are educational institutes where there is a requirement of affordable, user friendly, portable, energy efficient and secure automated system. Hence this prototype provides an amalgamated solution for replacing existing conventional attendance system with embedded attendance system. Main advantages are its very low cost, small size, efficient with low energy consumption.
Denial, anosodiaphoria, and emotional reactivity in anosognosia

Abstract A central flaw in models of anosognosia is to consider metacognitive awareness and affective regulation and responsiveness as separate functional modular subsystems. This line of reasoning leads to an “either or” conceptualization of the probable causes of implicit awareness in anosognosic patients. Neuroscience research and clinical observations of patients suggest that anosognosia is often associated with a change in the affective status of the individual as well as a change in their explicit verbal descriptions of themselves. Studying anosognosic patients over time and including measures of psychological denial and anosodiaphoria are necessary when interpreting markers of implicit awareness.