Miracle-dz/UniFAR-FTUs huggingface dataset:
Evaluation of Newly Established Cell Lines as Models to Study Growth, Invasion and Metastatic Spread in Oesophageal Cancer

Three oespohageal cell lines were established from two squamous carcinomas and one from an adenocarcinoma. Each cell line expressed both cytokeratin and vimentin intermediate filaments. They exhibited clonogenic growth in serum free media and grew in an anchorage independent fashion in soft agar. Each cell line produced both mitogenic and transforming growth factor activities. All three cell lines were invasive in the in vitro chick heart fragment invasion assay, albeit to different degrees, with OC2 cells showing the highest degree of invasiveness. In tumourigenicity studies in athymic nude mice, all three cell lines induced primary subcutaneous tumours at the site of injection. OC1 and OC3 cells gave rise to metastases, while the OC2 cells, although the most rapidly growing, failed in every case to metastasise. OC1 and OC3 cells were found to express urokinase-type plasminogen activator (uPA), while OC2 cells did not. Our findings suggest a role for uPA in conferring metastatic potential on oesophageal carcinoma cells.
The Changes induced in the Anatomical Structure of Vicia Faba by the Absence of Boron from the Nutrient Solution

THE now chemical recognized elements to be essential more numerous for the than healthy was growth originally of plants supposed, are now recognized to be m re numerous than was ori inally sup osed, and although some must be supplied in considerable quantity, of others only a trace is required. Boron is an example of the latter type of element. Experiments at Rothamsted have shown that Vicia Faba (broad bean) and several other leguminous plants fail to complete their development in water or sand culture unless a trace of this element is supplied, i part H3BO3 in 25,000-12,500,000 parts of nutrient solution being sufficient (1, 4). In its absence characteristic symptoms are exhibited in both shoot and root. In the case of the broad bean the flower buds shrivel and fall off and the stem withers and blackens at the apex, the injury gradually travelling down the plant ; the root system is stunted, the laterals being few in number and often thickened. A comparison between the anatomical structure of such abnormal shoots and roots with those of the healthy plant is the subject of the present paper.
Generation Times and Modeling of Enterotoxin-Positive and Enterotoxin-Negative Strains of Clostridium perfringens in Laboratory Media and Ground Beef.

The growth of two enterotoxin-positive (ent+) and two enterotoxin-negative (ent-) strains of Clostridium perfringens were examined in two laboratory media, fluid thioglycollate and fluid thioglycollate with beef, and in autoclaved ground beef stored at 37, 41, 43, 46, and 48°C. There was no association between enterotoxigenicity and optimum growth temperature, which varied depending on strain and medium. Experimentally obtained values for generation times at 43°C were as low as 8.7 and 6.3 min in thioglycollate both for ent+ and ent- strains respectively. In autoclaved ground beef the shortest generation times, 7.1 min and 9.2 min, were obtained at 41 and 46°C for two ent+ strains. For ent- strains in autoclaved ground beef a generation time of 6.6 min was observed at 43°C. Temperature optima obtained experimentally were the same as those obtained by the Gompertz model. The rapid growth of the ent+ strains between 41 and 46°C underlines previous recommendations for proper holding and cooling of protein foods such as meat and poultry known to support the growth of this organism.
Chapter 1. Measuring Trade-offs: A Review of Studies of Costs of Reproduction in Lizards

[Extract] The life history of an organism may be defined as a co-adapted complex of traits (Stearns 1976, 1989a; Ballinger 1983). If life-history traits are coadapted, the concept of trade-offs is central to the understanding of life-history evolution. This is because the optimization of a single aspect of reproduction cannot occur except at the expense of other, related aspects. Thus, natural selection should produce phenotypes that are compromises between the costs and benefits of changing any paiticular character.
Feeding Potential of Nesidiocoris tenuis (Reuter) on the Twospotted Spider Mite, Tetranychus urticae Koch, under Laboratory Conditions1

ABSTRACT Tetranychus urticae Koch (Acari: Tetranychidae) is one of the most important insect pests of tomatoes in greenhouses as well as open cultivation throughout the world. Nesidiocoris tenuis (Reuter) (Hemiptera: Miridae) is a polyphagous predatory bug, which is commonly found in tomato ecosystems. The present study was aimed at studying the predatory potential of N. tenuis against T. urticae. Results of this study indicate that all stages of the bug are highly predacious against T. urticae. Male nymphs consumed 11.8, 55.5, 66.3, 75.3, and 78.3 mites at the rate of 5.9, 18.5, 33.2, 37.6, and 39.1 mites per day for instars 1–5, respectively. The rate of consumption by female nymphs was 15.9, 61.8, 81.1, 88.8, and 90.1 mites at the rate of 7.9, 20.6, 40.6, 44.4, and 43.0 mites per day for instars 1–5, respectively. The overall consumption by female bugs (1137–1430 mites) was also higher than that of males (858–1092 mites). Augmentative releases of later instar nymphs of N. tenuis against T. urticae might therefore assure better mite control.
Evaluation of cytotoxic effects and antimicrobial activities of Lecaniodiscus cupanioides (Planch.) leaf extract

The present study assessed the cytotoxic and antimicrobial activities of Lecaniodicus cupanioides leaf extract, evaluated its killing rate and also determined the presence of certain phytochemicals. The antimicrobial activities were assessed via a susceptibility test and by determination of minimum bacteriotastic and bactericidal concentrations. The killing rate was evaluated at regular intervals over a 2h-period under different concentrations and the cytotoxicity was measured through a brine shrimp lethality test. Zones of inhibition exhibited by the extract at 20 mg/ml ranged between 20±0.00 and 23±0.58 mm against both bacteria and yeast isolates. The minimum inhibitory concentrations (MICs) were between 1.25 and 2.50 mg/ml against bacteria and 0.63 and 1.25 mg/ml against yeasts. The minimum bactericidal concentrations (MBCs) ranged from 2.50 to 5.00 mg/ml (bacteria) and 1.25 to 2.5 mg/ml (yeasts). At a concentration of 1×MIC after 15 min of contact time, the mortality against Shigella sonnei (ATCC 29930), Staphylococcus aureus (ATCC 6538) and Candida albicans as representative isolates were 52.3, 60.0 and 40.0%, respectively. Total mortality was achieved against S. sonnei (ATCC 29930) at a concentration of 2×MIC after 120 min of contact time while that of S. aureus (ATCC 6538) was achieved after 90 min of contact time at 3×MIC. Percentage mortality against the C. albicans after 120 min under a concentration of 3×MIC was 94.4%. The lethality concentrations at LC50 and LC90 of the leaf extract were 4.255 and 36.766 µg/ml, respectively. The results obtained support the therapeutic significance of L. cupanioides leaf extract and could be a veritable source of affordable broad spectrum antimicrobial and anti-tumour agent.
Genetic regulation of tomato fruit ripening and development and implementation of associated genomics tools

Fruit ripening has direct implications to a significant component of human diets, nutrition and agriculture. While ripening brings about positive changes in fruit physiology and chemistry in terms of flavor, appearance, texture and nutrition, over-ripening leads to post-harvest loss and decreased fruit quality. Researchers have studied numerous species with the intent of identifying strategies and technologies toward improving desirable ripening attributes while minimizing those with negative consequences. Tomato has emerged as a model for fleshy fruit ripening, in. part due to simple genetics, numerous characterized mutants, cross-fertile wild germplasm to facilitate genetic studies and routine transformation technology. In recent years, the tomato system has been further complemented with dense genetic maps, large EST collections and the recently initiated genome sequencing effort. Here we summarize recent advances in understanding the genetic regulation of fruit ripening and current and developing genomics tools that will impact this model system in the coming decade.
Domain-Based Prediction and Analysis of Protein-Protein Interactions

Many methods have been proposed for inference of protein-protein interactions from protein sequence data. This chapter focuses on methods based on domain-domain interactions, where a domain is defined as a region within a protein that either performs a specific function or constitutes a stable structural unit. In these methods, the probabilities of domain-domain interactions are inferred from known protein-protein interaction data and protein domain data, and then prediction of interactions is performed based on these probabilities and contents of domains of given proteins. This chapter overviews several fundamental methods, which include association method, expectation maximization-based method, support vector machine-based method, and linear programmingbased method. This chapter also reviews a simple evolutionary model of protein domains, which yields a scalefree distribution of protein domains. By combining with a domain-based protein interaction model, a scale-free distribution of protein-protein interaction networks is also derived.
3.2 A 7.6mW 1GS/s 60dB SNDR Single-Channel SAR-Assisted Pipelined ADC with Temperature-Compensated Dynamic Gm-R-Based Amplifier

Continuous technology scaling has allowed unceasing growth of the sampling rate of a single channel ADC in past decades. Such development not only helps reduce the number of channels in massively time interleaved ADCs, but also contributes to lower their overall jitter and input capacitance, thus enabling a further push on the ADC performance boundary. Being limited by metastability, the conventional SAR architecture is not suitable when both high resolution and speed are essential. While the pipeline SAR offers a higher speed alternative, it also keeps the low and dynamic power nature of the SAR architecture through adopting dynamic or integrating residue amplifiers (RAs) in recent works [1, 2]. With an integrating characteristic, the amplification time can be short but in contrast, the linearity, extra reset time of the load, and PVT sensitivity pose significant design challenges. In this work, rather than adopting the integratingtype amplifier, we present a Gm-R based RA which has a complete-settled amplification characteristic, thus allowing us to compensate the gain variation over temperature easily with a tracking bias technique. Besides this, we use a two-stage amplification to alleviate the RA input parasitic capacitance that enables a small DAC size in all stages. The single channel prototype reaches 1GS/s with 60.02dB SNDR at a Nyquist input consuming 7.6mW from a 1V supply.
Carotenoid, chlorophyll and chlorophyll-derived compounds in pistachio kernels (Pistacia vera L.) from sicily

The composition of carotenoid, chlorophyll, and chlorophyll-derived compounds in Sicilian pistachio kernels (Pistacia vera L.) was investigated. In all, 13 compounds were identified and quantified using reversed-phase liquid chromatography with photodiode array detection with a C-30 column. Internal kernel colour is an important visual quality characteristic for the food processing industry. Chlorophyll a was the major component (54.14 ppm), followed by chlorophyll b (30.2 ppm). Lutein (29.14 ppm) and pheophytin a (25.68 ppm) were also well represented. Pheophytins a and b, neoxanthin, luteoxanthin and violaxanthin were identified in pistachio nuts for the first time. The presence of a carotenoid ester was also detected. The ratio between the two isochromic pigment fractions, namely the chlorophyll and the carotenoid fractions, was 2.7, clearly showing the prevalence of the green colour; the chlorophyll a/chlorophyll b and the lutein/ β-carotene ratios were of 1.8 and 4.1, respectively. These parameters, along with other analytical parameters, could be used as indicators of typicality in Sicilian pistachio nuts. The presence of a specific pigment profile in nuts could, in fact, be used to guarantee the typicality of the product, since the quality control of food also requires a precise knowledge of the pigment composition of the original product.
Estimating Conformity of Head Yaw to the ICAO Standard using a Convolutional Neural Network

Face images are widely used in authentication documents to recognize individuals. Face quality assessment can be used to enhance the efficiency of face recognition algorithms. One of the most important challenges in the design of face image quality algorithms is the measurement of parameters such as darkness, light conditions, head position and facial expressions. In recent years, traditional identity documents have been replaced by electronic documents with biometrics traits. International Civil Aviation Organization (ICAO) has imposed some requirements for face image features, according to them any image used for biometric applications should be conform to these requirements. In this research, a model has been developed to measure the match between orientation of the head rotation around the vertical axis (Yaw), which is one of the requirements of ICAO (ISO / IEC19794 -11). The basis of our work is the use of convolutional algorithms and supervised learning in the field of deep learning methods. Using the reconfiguration of VGG-Face deep neural network and a combination of different databases, we have trained the deep learning model. Then, to compare the proposed method with the results of other articles, we tested the learned network on the PIE database and accurately identified 96.5% of the correct diagnosis. Finally, in order to test the genericity of the proposed method, we tested it on the CSIE Robotic database, where we obtained an accuracy of 97.8%.
Изучение взаимосвязи между электрическим сопротивлением и флуктуирующей ассиметрии листовой пластины березы повислой

Electric potentials in plant organisms have a close functional relationship with the physiological polarization and growth processes involved in the uptake and movement of nutrients in the plant. Of particular interest in this regard is the growth and development of leaves of Betulapendula in the conditions of technogenic pollution, in particular the dependence of the electrical resistance and fluctuating asymmetry of leaves. The measure of fluctuating asymmetry sheet is a method of confirming measurements using an electrometric method. The object served as the leaves of Betulapendula. Discusses key issues, showing the dependence of the electrical resistance of the sheet from the environmental growth conditions.The dependence of the distribution of electrical resistance and fluctuating asymmetry of leaf blade. According to a study, it was determined that the resistance of the leaf blades trees of Betulapendula depends on stressful situations, i.e. in the presence of oil pollution of soil electrical resistance of leaves increases under different hydrothermal regime of the air environment and is manifested on the anatomical and morphological characteristics of leaves of trees. It is established that with the increase in fluctuating asymmetry leaf blades increases the level of electrical resistance of the sheet.
Biodegradation of a BTX mixture by Pseudomonas strains: monitoring of two co-cultured strains by polymerase chain reaction of catabolic genes

Pseudomonas putida CM37, Pseudomonas aureofaciens CM32 and Pseudomonas putida CM23 degraded toluene through different pathways. CM37 carried xylA,M gene for xylene monooxygenase (TOL pathway), CM32 carried todC1 gene for toluene dioxygenase (TOD pathway) and CM23 carried both genes and was characterised by the contemporary presence of TOL and TOD pathways. In this study we compared the behaviour of these strains grown on a limiting concentration of a benzene, toluene and m-xylene (BTX) mixture as the carbon and energy source. Moreover, the competition between the two Pseudomonas strains CM37 and CM32 was monitored during BTX degradation when co-cultured in batch experiments by both colony culturing and molecular techniques. A polymerase chain reaction (PCR) quantitative method of enumeration was set up on xylA,M and todC1 catabolic genes. The two enumeration methods showed satisfactory agreement and revealed CM32 to be the most competitive strain when co-cultured with CM37 on the BTX mixture. Application of PCR detection of aromatic catabolic genes enabled to enumerate selectively bacterial strains preferring benzene, toluene or m-xylene.
The dual role of mannitol as osmoprotectant and photoassimilate in celery

ni n a . de us Mannitol, a six carbon noncyclic sugar alcohol, is the most a dant polyol in nature, occurring in bacteria, fungi, algae, lichens, in at least 70 species of vascular plants (Bieleski, 1982; Lewis, 1 Various roles have been postulated for mannitol in these organ including carbon storage, free radical scavenging, osmoregula and service as a compatible solute (Bieleski, 1982; Loescher, 1 Some of these roles are of obvious importance in stress toler particularly in tolerance to salt stress, which invariably cau osmostress of varying durations in plants. Mannitol is also a phlo translocated photoassimilate in some vascular plants (Bieleski, 1 Loescher, 1987). This article focuses on the enzymatic synthes degradation of mannitol in celery ( Apium graveolens L. var. dulce) plants and the role of these pathways in controlling mannitol accu lation in stressed plants.
Revision of the enigmatic crocodyliform Elosuchus felixi de Lapparent de Broin, 2002 from the Lower-Upper Cretaceous boundary of Niger: potential evidence for an early origin of the clade Dyrosauridae

The enigmatic crocodyliform ‘Elosuchus’ felixi from the Echkar Formation (upper Albian to lower Cenomanian, Early–Late Cretaceous boundary) west of In Abangharit, Agadez District, Niger, is here re-described. Our assessment of the material shows that there are at least two taxa amongst the referred material: ‘E. ’ felixi, including the holotype (an incomplete lower jaw) and two larger incomplete lower jaws; and an incomplete premaxilla, which we refer to Elosuchus sp. All other referred material is herein considered Crocodyliformes indeterminate. Based on our study of ‘E. ’ felixi we refer it to a new genus, Fortignathus. A comparative study and updated phylogenetic analyses both suggest that F. felixi comb. nov. is a non-hyposaurine dyrosaurid or a dyrosaurid sister taxon. This is supported by four characteristics, including: inferred double festooned maxillae, a large gap between the D2 and D3 alveoli, gladius-shaped anterior dentary, and enlarged D4 alveoli that have a subrectangular cross section. The paucity of material means we refrain from referring F. felixi comb. nov. to Dyrosauridae. This species and suggestive material from the Cenomanian of Sudan allows us to formulate two hypotheses, however: (1) basal dyrosaurids were either freshwater or could live in both freshwater and saltwater ecosystems; and (2) Africa was their place of origin and dispersal.
Riqueza de espécies de plantas visitadas por abelhas na Universidade

Estudos de ecologia reprodutiva de plantas em ambientes urbanos sao particularmente importantes, pois podem avaliar o potencial adaptativo de uma especie presente em area urbanizada ou o processo de naturalizacao de uma especie exotica no Brasil. Estudos sobre biologia da polinizacao envolvem conhecimentos sobre fenologia e biologia floral, bem como morfologia e comportamento de visitantes as flores. Ha poucos estudos com a fenologia floral de plantas ornamentais, pois geralmente sao especies exoticas. O objetivo desse estudo foi verificar a riqueza das especies de plantas visitadas por abelhas na Universidade Metodista de Piracicaba. As observacoes das especies de plantas em flor e analises laboratoriais foram realizadas no periodo de agosto de 2009 a julho de 2010. Foram registradas 54 especies de plantas arboreas e arbustivas, sendo que 52% sao nativas. A familia Leguminosae foi a mais representativa. Houve plantas florescendo em todos os meses, mantendo os recursos, polen e nectar, disponiveis ao longo do periodo de observacao. Houve predominância das especies com flores brancas, tipo floral aberto e antera com deiscencia longitudinal. Substâncias odoriferas que sao utilizadas para atracao das abelhas estao presentes em 80% das especies. Informacoes sobre a fenologia e a biologia floral podem ser uteis na manutencao da arborizacao ornamental de ambientes urbanos, mantendo disponiveis os recursos necessarios aos visitantes. Palavras-chave : Biologia floral. Ecologia urbana. Melitofilia. Plantas ornamentais.
Enfezamentos vermelho e pálido: Doenças em milho causadas por molicutes Maize bush stunt and corn stunt: Diseases of corn caused by molicutes

Resumo: Os enfezamentos vermelho e palido sao doencas do milho causadas, respectivamente, por um fitoplasma (“maize bushy stunt phytoplasma”) e pelo Spiroplasma kunkelii, organismos restritos ao floema das plantas infectadas. Ambas sao transmitidas pela cigarrinha Dalbulus maidis. Sao doencas bastante destrutivas, podendo causar serios prejuizos aos agricultores. A importância dessas doencas aumentou muito nos ultimos anos, devido principalmente aos cultivos “safrinha”, que perpetuam o milho, os patogenos e o vetor no campo durante o ano todo. As plantas doentes exibem reducao de crescimento, avermelhamento ou amarelecimento, proliferacao excessiva de espigas pequenas e improdutivas, alem de outros sintomas. No entanto, esses sintomas dependem do agente causal, condicoes climaticas e genotipo do milho. A diagnose correta tem sido feita por testes de ELISA (“enzyme-linked immunosorbent assay”) e por PCR (“polymerase chain reaction”), devido a complexidade da sintomatologia. Nas areas onde ocorrem, o controle dessas doencas baseia-se principalmente no uso de genotipos resistentes de milho. Palavras-chave: Enfezamento, molicutes, milho, Spiroplasma kunkelii, phytoplasma.
Effect of salt stress on mitochondrial energy metabolism of Vigna unguiculata cultivars differing in NaCl tolerance

Mitochondria have been purified from hypocotyls of two cultivars of Vigna unguiculata (L.) Walp, known to be sensitive (Vita 5) and tolerant (Vita 3) to salinity. For Vita 5 mitochondria, 0.1 M NaCl treatment resulted in a lowering of the phosphorylative efficiency, while the mitochondrial ATPase activity was not modified. By contrast, stimulation of the ATPase activity in Vita 3 mitochondria under salt stress was in good agreement with the maintenance of the phosphorylative properties of the mitochondria. Under salt stress, the enzyme displays a lower affinity for the substrate (MgATP) and a higher Vmax. Consequently, under stress conditions, the two cultivars could be distinguished by the level of ATPase activity in the hypocotyl mitochondria