Miracle-dz/UniFAR-FTUs huggingface dataset:
Gravity‐sensitive signaling drives 3‐dimensional formation of multicellular thyroid cancer spheroids

This study focused on the effects induced by a random positioning machine (RPM) on FTC‐133 thyroid cancer cells and evaluated signaling elements involved in 3‐dimensional multicellular tumor spheroid (MCTS) formation. The cells were cultured on the RPM, a device developed to simulate microgravity, and under static 1‐g conditions. After 24 h on the RPM, MCTSs swimming in culture supernatants were found, in addition to growth of adherent (AD) cells. Cells grown on the RPM showed higher levels of NF‐κB p65 protein and apoptosis than 1‐g controls, a result also found earlier in endothelial cells. Employing microarray analysis, we found 487 significantly regulated transcripts belonging not only to the apoptosis pathway but also to other biological processes. Selected transcripts were analyzed with quantitative real‐time PCR using the same samples. Compared with 1‐g IL‐6, IL‐8, CD44, and OPN were significantly up‐regulated in AD cells but not in MCTSs, while ERK1/2, CAV2, TLN1, and CTGF were significantly down‐regulated in AD cells. Simultaneously, the expression of ERK2, IL‐6, CAV2, TLN1, and CTGF was reduced in MCTSs. IL‐6 protein expression and secretion mirrored its gene expression. Thus, we concluded that the signaling elements IL‐6, IL‐8, OPN, TLN1, and CTGF are involved with NF‐κB p65 in RPM‐dependent thyroid carcinoma cell spheroid formation. —Grosse, J., Wehland, M. , Pietsch, J., Schulz, H. , Saar, K. , Hübner, N. , Eilles, C. , Bauer, J., Abou‐El‐Ardat, K. , Baatout, S. , Ma, X. , Infanger, M. , Hemmersbach, R. , Grimm, D. Gravity‐sensitive signaling drives 3‐dimensional formation of multicellular thyroid cancer spheroids. FASEB J. 26, 5124–5140 (2012). www.fasebj. org
In vitro activation of the 20S proteasome.

The effect of chemical compounds like sodium dodecyl sulfate (SDS), fatty acid esters of glycerol, carnitine and coenzyme A, phospholipids, histones, polylysines as well as homobifunctional chemical cross-linkers on the various proteolytic activities of mammalian proteasomes have been tested. Most of the reagents enhance these activities, and some, e.g. fatty acid CoA esters, histones and the chemical cross-linkers, exert dual effects, i.e. activation and inhibition at the same time, depending on the activity measured. With optimally activating concentrations of SDS, no structural changes in proteasomes can be detected by electron microscopy. Formation of micelles at supra-optimal detergent concentrations may be a reason for irreversible denaturation of the proteasome.
value co-creation through online communities in the context of new social media sites

Companies are using online communities (OCs) as a mean of value creation for some time. Over time, much has been written both by academics and nonacademics about this subject. However, there is a need for a more systematic understanding of the processes of value creation through these online communities. First, extant literature is scattered by several sources and uses different terminologies. Second, the social web field is in constant change and evolution. Through a generic qualitative study, which entailed an iterative process between literature review, data collection and data analysis, a taxonomy was built. We have identified four main distinct processes of value creation with consumers though online communities. The first way of value creation consists of developing online community-based business models, were value is translated into direct revenue. The second involves creating consumer communities as a supplement of an existing business in order to influence consumption processes and to involve consumers in co-production. With similar objectives, the third entails connecting with already existing consumer-run online communities. Finally, the fourth involves taking advantage of the online communities created by other companies. Each of these categories is then describe and decomposed into more specific processes. We finish by presenting research and managerial implications of this study. Keywords—online community; consumer community; brand community; value creation; social web
Antimicrobials Resistance: A Call for Multi-disciplinary Action. How Can HTA Help?

Antimicrobial resistance (AMR) is in part a natural phenomenon, but its growth has been accelerated over time by different causes that now exert a cumulative effect. On the one hand, bad practice favours the spread of infections, such as overuse of antibiotics in human and veterinary care, and poor prevention and hygiene practices. On the other hand, the scarcity of new treatments, both recently launched as well as in pharmaceutical industry development pipelines, reduces the chances of treating AMR effectively in the future. This publication is a report of a symposium held at the HTAi 2017 meeting in Rome. Throughout the session, the speakers provided recommendations for the strategies and actions that should be implemented in order to prevent the spread of AMR and to incentivise the development of new effective treatments.
Actividad antioxidante y neuroprotectora in vitro del extracto acuoso del alga Bryothamnion triquetrum (Ceramiales, Rhodomelaceae)

La presente investigacion aborda el estudio de las propiedades antioxidantes y neuroprotectoras de un extracto acuoso del alga Bryothamnion triquetrum (S.G.Gmelin) Howe, asi como la relacion de tales propiedades con los compuestos fenolicos presentes en este producto natural, mediante el uso de modelos experimentales in vitro. Los resultados mostraron que el extracto acuoso de B. triquetrum contiene moleculas que pueden unir Fe, atrapar radicales O2•, OH•, DPPH•, e inhibir la peroxidacion lipidica espontanea e inducida quimicamente en homogenados de tejido cerebral de rata. Dentro de los componentes quimicos con propiedades antioxidantes conocidas, fue determinado que el extracto contiene Se, acido ascorbico, carotenos y compuestos fenolicos. Se profundizo particularmente en la composicion de compuestos fenolicos del extracto de B. triquetrum, utilizando una combinacion de extracciones liquido- liquido, Cromatografia en Capa Delgada preparativa y Cromatografia en Columna de Amberlite XAD-2. Ello permitio obtener fracciones del extracto con actividad inhibitoria sobre la peroxidacion lipidica espontanea en homogenados de tejido cerebral de rata, en las que se identificaron y cuantificaron por Cromatografia Gaseosa, los acidos ferulico, p-cumarico y trans-cinamico, que representan el 60% de los compuestos fenolicos totales del extracto. La actividad neuroprotectora del extracto y los acidos cinamicos que contiene, fue estudiada en celulas inmortalizadas de hipotalamo de raton GT1-7 expuestas a diferentes condiciones experimentales en que se indujo estres oxidativo. La citotoxicidad producida por H2O2, la combinacion de H2O2 y FeSO4, SIN-1, MeHg y la combinacion de hipoxia quimica y aglucemia en diferentes condiciones de exposicion, fue significativamente atenuada por la exposicion al extracto de B. triquetrum y ello pudo relacionarse, al menos parcialmente, con su contenido de acido ferulico. Ademas, fue demostrado que los acidos p-cumarico y trans-cinamico no son responsables de los efectos neuroprotectores observados en neuronas GT1-7 ni incrementan sinergicamente la actividad registrada para el acido ferulico. Con relacion a los posibles modos de accion involucrados, fue demostrado que el efecto neuroprotector del extracto puede o no involucrar la disminucion en la produccion de radicales libres, pero no se relaciona con el incremento en las reservas intracelulares de GSH.
On the use of MOS varactors in RF VCOs

This paper presents two 1.8 GHz CMOS voltage-controlled oscillators (VCOs), tuned by an inversion-mode MOS varactor and an accumulation-mode MOS varactor, respectively. Both VCOs show a lower power consumption and a lower phase noise than a reference VCO tuned by a more commonly used diode varactor. The best overall performance is displayed by the accumulation-mode MOS varactor VCO. The VCOs were implemented in a standard 0.6 /spl mu/m CMOS process.
Preventing the Data Over-Collection in Smart City via Secure Protection

: The smart city is meant to be the next generation of Urbanization. The most arduous challenge about the cyber security and privacy of smart city to ensure the precise data secure. The smart device not only stores users' data but also generates data. These data may consist of users' accounting numbers and passwords, emails and house addresses, photos, and other kinds of precise data. The most serious possible security hazard is that applications collect data more than enough on its original function while in permission scope, which to call it data over-collection. Cloud services are in charge of managing users' data and provide fine-grained access control and encryption/ decryption services. To solve the problem of quantifying security risk to score application mainly focusing about the data over-collection behaviors. To prove that the framework improves the security of Smartphone’s significantly.
Social Responsibility for Labor Productivity in Companies

The objective of the research was to determine the relationship of social responsibility with labor productivity in companies. This allows establishing a link that facilitates the identification of flaws in the organization with respect to work performance and its commitment to the welfare of society. For this, questionnaires were applied to 85 workers of the company Quala S.A. of Colombia and the Distribuidora de Consumo Masivo Rave C. A of Venezuela, which were analyzed with the Pearson coefficient. This resulted in a coefficient of r = 0.88, denoting a strong positive relationship for the variables Social responsibility and Labor production, However, there are problems for companies regarding aspects such as workers' compensation and job stability, and they do not comply with economic, legal, ethical and environmental levels of responsibility. Keyword Labor performance, Production, Management, Environment, Society
Exploring the Individual's Behavior on Self-Disclosure Online

Even though the ubiquity of technology can make many aspects of life more convenient, it may simultaneously raise some concerns about the privacy issues related to personal information. This research focused on the individual's behavior toward self-disclosure online and his or her cognition on personal privacy that was affected by not only the personal psychological factors but also the individual's computer knowledge and associated skills. To study how technological and psychological factors can simultaneously affect one's privacy concern and restrain behavior on disclosure, this study applied both perceived privacy and privacy concern to assess such factors as personal subjective attitude, computer self-efficacy, and perceived behavioral control to investigate the technological influence. A hypothesized model was proposed to describe the relationships among these constructs. After structural equation modeling is utilized to analyze the proposed hypotheses, the research findings showed that, although the perceived behavioral control had a negative effect on self-disclosure online, all the other hypotheses indicated a positive casual effect.
Cardiac Power Index, Mean Arterial Pressure, and Simplified Acute Physiology Score II Are Strong Predictors of Survival and Response to Revascularization in Cardiogenic Shock

ABSTRACT Short-term prognostic factors in patients with cardiogenic shock (CS) have previously been established using only hemodynamic parameters without taking into account classic intensive care unit (ICU) severity score or organ failure/support. The aim of this study was to assess early predictors of in-hospital mortality of a monocentric cohort of patients with ST-elevation myocardial infarction complicated by early CS. We retrospectively studied 85 consecutive patients with CS complicating acute myocardial infarction and Thrombolysis in Myocardial Infarction flow grade 3 after percutaneous coronary revascularization. All patients were managed according to the following algorithm: initial resuscitation by a mobile medical unit or in-hospital critical care physician unit followed by percutaneous coronary revascularization and CS management in the ICU. Prehospital CS was diagnosed in 69% of cases, initially complicated by an out-of-hospital cardiac arrest in 64% of cases. All patients were treated with vasopressors, 82% were ventilated, and 22% underwent extrarenal epuration. The 28-day mortality rate was 39%. Under multivariate analysis, initial cardiac power index, mean arterial pressure of less than 75 mmHg at hour 6 of ICU management, and Simplified Acute Physiology Score II were independent predictive factors of in-hospital mortality. In conclusion, parameters directly related to cardiac performance and vascular response to vasopressors and admission Simplified Acute Physiology Score II are strong predictors of in-hospital mortality.
Sequential neuromotor examination in children with intrauterine cocaine/ polydrug exposure

Data are presented on 157 newborn infants followed sequentially in a randomized home‐based nursing‐intervention trial for drug‐exposed infants with follow up at 3 (N=118), 6 (N=124), and 12 months (N=77). The objectives of this study were to describe the longitudinal neurodevelopmental status of a cohort of children with intrauterine exposure to illicit drugs during their gestation, characterize the evolution of early tone abnormalities in a polydrug‐exposed cohort, and determine whether neuromotor outcome is associated with drug‐exposure patterns. For analysis, infants were grouped based on maternal drug‐use pattern and the presence of drug metabolites in the neonatal drug screen. The sequential neuromotor examination was used at each age to define the neuromotor status of six domains and define categorical classifications as either normal, suspect, or abnormal. Multiple patterns of neuromotor abnormalities were observed during the neonatal period; most resolved over time. Axial hypotonia was a prominent finding in the neonatal period; however, it was infrequent in abnormal examinations at 12 months. Increased lower‐extremity tone was a less frequent finding during the neonatal period. Infants whose neonatal urine drug screen was positive for both cocaine and opiates, were more likely than infants with negative urine drug screens, cocaine only, or opiate only drug screen results to have abnormal neuromotor examinations; while positive maternal drug screens for concurrent cocaine and opiate use were associated with peripheral hypertonia. Persistence of increased leg‐extensor tone was found in 67% of the abnormal examinations at 12 months. Acquisition of rolling and walking was delayed in the drug‐exposed cohort.
Fibrinogen Levels in Hypertensive and Normotensive: A Cross-Sectional Study from El-Obied City, Sudan

Hypertension (HTN) has been defined as a blood pressure level at which an otherwise healthy person would have an increased risk of cardiovascular disease that could be mitigated through blood pressure-lowering treatment. Worldwide, hypertension is one of the most common causes of death. The prevalence of hypertension and cardiovascular disease increases with age, and has been found to be higher in those of South Asian and African ancestry, and in Aboriginal populations. Hypertension is not only one of the most important risk factors for cardiovascular disease, but also the number one modifiable risk factor for stroke. Alterations in blood coagulation system have been reported in patients of hypertension. Fibrinogen has been identified as a major independent risk factor for cardiovascular diseases. This was a descriptive cross-sectional study, conducted in Elobied teaching hospital during period from March to June 2014. The aim of this study is to determine the level of fibrinogen among hypertensive patients. Seventy percent of the patients (about 35 patients) had high fibrinogen level above 400 mg/dl compared with the control and about 30% (15 patients) were within the normal range of 200 mg - 400 mg/dl. This study has concluded that there was a significant increase in fibrinogen level in hypertensive patients compared to control, while the gender has no effect on the level of fibrinogen.
Red cell deformability and its relevance to blood flow.

The resistance to blood flow is a function of the rheological properties of blood as well as the geometric features of blood vessels. While circulatory investigations generally aim at studying the functions of the heart and blood vessels, there is an increasing realization that blood rheology can also play a significant role in circulatory regulation in health and disease (10, 11). The principal factors determining blood viscosity are hematocrit, plasma viscos­ ity, cell aggregation, and cell deformability (8). This review focuses on the role of the deformability of the red blood cell (RBC) in circulatory regulation under physiological and pathophysiological conditions. 1 Deformability is a relatively general term that describes the ability of a body (RBCs in this case) to change its shape in response to a deforming force. RBC deformation (or strain) can involve a change in cell curvature, a uniaxial deformation, or area expansion (3, 33). The deforming stress (force per unit area) is usually applied from outside the cell, e.g. by fluid shear stress or local membrane aspiration, but it can also originate from within the cell, e.g. RBC swelling in hypotonic medium or fiber formation in deoxygenated sickle cells. The extent, rate, and mode of deformation depend on the magnitude, rate, and direction of the stress. Therefore, the stress-strain (and stress-strain rate) relationships are complex and cannot be described by a single deformability parameter.
Use of alkaline sucrose gradients in a zonal rotor to detect integrated and unintegrated avian sarcoma virus-specific DNA in cells

We have attempted to distinguish integrated and unintegrated forms of avian sarcoma virus-specific DNA in cells by sedimentaton through an alkaline sucrose gradient in a slowly reorienting zonal rotor. Results obtained with this procedure are similar to those obtained by the more convenient analysis of networks of high-molecular-weight cell DNA. Most, if not all, viral DNA appears completely integrated into the host cell genome in an avian sarcoma virus-transformed mammalian cell and in normal chicken cells (in which viral DNA is genetically transmitted). Fully transformed duck cells and duck embryo fibroblasts infected for 20 to 72 h contain both integrated and unintegrated viral DNA; up to one copy per cell is integrated within 20 h after infection, and four to eight copies are integrated in fully transformed cells. The amount of unintegrated DNA varies but may comprise over 75% of the viral DNA in acutely infected cells and from 20 to 70% of the viral DNA in fully transformed cells. The unintegrated DNA in either case consists principally of duplexes with "minus" strands the length of a subunit of the viral genome (2.5 X 10(6) to 3 X 10(6) daltons) and relatively short "plus" strands (0.5 X 10(6) to 1.0 X 10(6) daltons).
Flow identification for supporting per-flow queueing

Over the last several years there has been considerable interest in multimedia communications over the Internet. This paper investigates one of the key technical issues in supporting per-flow queueing-flow identification. We present several hashing-based flow identification approaches and examine their performance and scalability limits with a quantitative analysis and a simulation study with backbone traffic traces. Of the hashing schemes we studied, 32-bit CRC and XOR-folding of the five-tuple demonstrate excellent performance, both on the memory requirement for a collision rate target, and on the number of collided flows on average and in the worst-case. Our findings show that, with hashing-based schemes, it is feasible to implement flow identification at high speeds to support hundreds of thousands of flows with resource reservation.
The Effect of Quality of Institutions on Sector Level Foreign Direct Investment

This study addresses the issue whether institutional quality affects the sector-wise FDI both in short run and long run in Pakistan. By employing ARDL co-integration technique, we analyze the impact of institutional quality on primary, manufacturing and services sectors FDI in Pakistan. The findings suggest that institutional quality matters in attracting FDI in manufacturing and services sectors in the long run while institutions do not have a significant impact on FDI in the primary sector. Moreover, results show that the impact of institutional quality on these sectors is not apparent in short run.
Title Expression profiling in transgenic FVB / N embryonic stem cellsoverexpressing STAT 3 . Author ( s )

The transcription factor STAT3 is a downstream target of the LIF signalling cascade. LIF signalling or activation is sufficient to maintain embryonic stem (ES) cells in an undifferentiated pluripotent state. To further investigate the importance of STAT3 in maintaining the pluripotency of ES cells we have generated transgenic FVB mice expressing a conditional tamoxifen dependent STAT3-MER fusion protein. Transgenic blastocysts have yielded pluripotent germ line competent ES cells at high frequency when established in a tamoxifen-containing medium in the absence of LIF. The expression profiling of tamoxifen induced transgenic FVB ES cell lines when compared with wild type cells has revealed a set of genes that were markedly upor down-regulated. The expression of four of the up-regulated genes (Hexokinase II, Lefty2, Pramel7, PP1rs15B) was shown to be restricted to the inner cell mass (ICM) of blastocysts. These differentially expressed genes represent potential candidates for the maintenance of pluripotency of ES cells. We finally overexpressed two of the identified genes, Pem and Pramel7, in embryonic stem cells and demonstrated that their overexpression is sufficient for maintaining the expression of markers of the undifferentiated, pluripotent state and typical morphology of pluripotent ES cells in absence of LIF.
Prostate cancer screening attitudes and continuing education needs of primary care physicians.

BACKGROUND In May 2003, a survey questionnaire was distributed to all licensed primary care physicians in Newfoundland and Labrador. The objective was to examine the attitudes, self-reported practices, and continuing medical education (CME) preferences of primary care physicians as they pertain to prostate cancer screening. METHODS Data was obtained from 485 primary care physicians using self-reports of prostate cancer screening practices, attitudes towards prostate cancer screening, and CME preferences. Respondents' characteristics were also collected (eg, gender, years of experience). RESULTS A majority of respondents screen asymptomatic male patients for prostate cancer. Screening behaviour was related to high volume practice settings, fee-for-service and increased with patient age. Most common reasons for screening were family history, age of patient, and patient request. Majority of physicians agreed that prostate screening should be routinely performed on all men beginning at age 50, however half of physicians believe there is lack of evidence to support digital rectal examination (DRE) and one-third of physicians do not believe the prostate-specific antigen (PSA) nor DRE are accurate screening tests. Areas of greatest interest for CME included topics related to prostate cancer screening effectiveness, strategies for prevention, sexual dysfunction, available treatments and their side effects, and management options. CONCLUSION Physicians are supportive of the value of screening, however the reliability of and evidence to support DRE and PSA as prostate cancer screening tests are in question. CME which addresses issues surrounding prostate screening and areas related to patient education and counselling are of greatest need.