Miracle-dz/UniFAR-FTUs huggingface dataset:
Resistance and tolerance of ten carrot cultivars to the hawthorn-carrot aphid, Dysaphis crataegi Kalt., in Poland

Damage caused to cultivated carrots by the hawthorn-carrot aphid, Dysaphis crataegi Kalt. (Hemiptera: Aphididae) is one of the factors limiting carrot production in Poland. Planting resistant and tolerant cultivars could reduce yield losses due to the damage caused by this pest. This study was conducted to evaluate the resistance and/or tolerance of 10 carrot genotypes to hawthorn-carrot aphid. Their field resistance was determined under field conditions based on five indicators, namely, mean number of alates (migrants) per plant and mean percentage of plants colonized by them, mean seasonal number of aphids per plant, mean number of aphids per plant and mean percentage of infested plants at peak abundance. Antibiosis experiments were conducted under laboratory conditions and pre-reproductive, reproductive time, fertility, and demographic parameters, represented by the net reproduction rate (Ro), intrinsic rate of increase (rm) and mean generation time (T), were calculated. Five cultivars, Afro F1, Nipomo F1, Samba F1, White Satin F1, and Yellowstone showed field resistance. Antibiosis experiments revealed significant differences among the carrot cultivars in the length of the reproductive period, female fecundity in the time equal to the pre-reproduction time, and total progeny of hawthorn-carrot aphid. The intrinsic rate of natural increase (rm) for apterous aphids varied significantly, ranging between 0.181 (Nipomo F1) and 0.343 females/female/day (White Satin F1). Additionally, the estimated net reproductive rate (R0) was the lowest on Nipomo F1, and this genotype was determined to be resistant. Our results suggest that a very high density of trichomes on the leaf petioles (71.94 trichomes/cm2) could adversely affect the feeding, bionomy, and demographic parameters of hawthorn-carrot aphid on the cultivar Nipomo F1. In addition, Napa F1 and Kongo F1 demonstrated high tolerance. Considering all the results collectively, four genotypes, Afro F1, Kongo F1, Napa F1 and Nipomo F1, were relatively resistant/tolerant to the hawthorn-carrot aphid.
Control over the nucleation process determines the framework topology of porous coordination polymers

In this contribution, we present a method to selectively synthesize crystal polymorphs of [Zn2(bdc)2(dabco)]n (1) and [Zn2(bdc)2(bpy)]n (2), either the pillared 2D square-grid nets (1sql or 2sql) or the pillared 2D Kagome nets (1kgm or 2kgm) by simply changing the crystallization temperature.
From Theory to Practice: Bone Health in Women with Early Breast Cancer Treated with Aromatase Inhibitors

Aromatase inhibitors (AI) are extensively used as adjuvant endocrine therapy in post-menopausal women with hormone receptor-positive early breast cancer (HR+ EBC), but their impact on bone health is not negligible. This work aimed to assess bone loss, fracture incidence, and risk factors associated with these events, as well as the prognostic influence of fractures. We have conducted a retrospective cohort study of women with HR+ EBC under adjuvant therapy with AI, during a 3-year period. Four-hundred-and-fifty-one eligible women were reviewed (median age 68 years). Median time under AI was 40 months. A fracture event occurred in 8.4%, mostly in the radium and femoral neck and in older women (mean 74 vs. 68 years, p = 0.006). Age (OR 1.01, 95% CI 1.01–1.07, p = 0.024) and time under AI (OR 1.02, 95% CI 1.00–1.04, p = 0.037) were independent predictors of fracture, with a fair discrimination (AUC 0.71). Analysis of disease-free survival according to fracture event varied between groups, disfavoring the fracture cohort (at 73 months, survival 78.6%, 95% CI, 47.6–92.4 vs. 95.6%, 95% CI, 91.2–97.8, p = 0.027). The multivariate model confirmed the prognostic impact of fracture occurrence (adjusted HR of 3.17, 95% CI 1.10–9.11; p = 0.032). Bone health is often forgotten, despite its great impact in survivorship. Our results validate the pathophysiologic link between EBC and bone metabolism, which translates into EBC recurrence. Further research in this area may help refine these findings. Moreover, early identification of women at higher risk for fractures is warranted.
Significance of ocean carbonate budgets for the global carbon cycle

Changes in the trace gas composition of the atmosphere over glacial–interglacial cycles are linked to changes in the oceanic carbon cycle. This paper examines the role of biologically driven fluxes of organic and inorganic carbon in modifying the carbon dioxide chemistry of the oceans, and the corresponding implications for the partitioning of CO2 between the atmosphere and ocean. Relevant details of the marine carbon system are presented together with an assessment of the significance of remineralization and dissolution processes. Recent estimates of the marine carbonate fluxes show significant uncertainties and inconsistencies which must be resolved in order to assess fully the role of the oceans' biota in the marine carbon system. Various types of ocean carbon cycle models have been developed in order to interpret the changes in past atmospheric carbon dioxide. Some take account of the role of the oceans' biota, focussing in the main on the cycling of organic matter. Relatively few have considered the role of the carbonate pump and the subtle interactions between organic and inorganic carbon cycling. The significance of carbonate formation and dissolution, and of the effects of global change on the marine carbonate system, for air–sea fluxes of CO2 are discussed. Finally some recommendations for future research are made in order to improve our understanding of how spatial and temporal variation in marine carbonate fluxes, in conjunction with processes determining the oxidation and burial of organic matter in the oceans, affect levels of CO2 in the atmosphere.
Posttraumatic Stress Disorder Subsequent to Apparent Mild Traumatic Brain Injury

Posttraumatic stress disorder (PTSD) is prevalent among veterans with a history of traumatic brain injury (TBI); however, the relationship between TBI and PTSD is not well understood. We present the case of a 31-year-old male veteran with PTSD who reported TBI before entering the military. The reported injury appeared to be mild: He was struck on the head by a baseball, losing consciousness for ∼10 seconds. Years later, he developed severe PTSD after combat exposure. He was not receiving clinical services for these issues but was encountered in the context of a research study. We conducted cognitive, autonomic, and MRI assessments to assess brain function, structure, and neurophysiology. Next, we compared amygdala volume, uncinate fasciculus diffusion, functional connectivity, facial affect recognition, and baroreceptor coherence with those of a control group of combat veterans (n = 23). Our veteran’s MRI revealed a large right medial–orbital prefrontal lesion with surrounding atrophy, which the study neuroradiologist interpreted as likely caused by traumatic injury. Comparison with controls indicated disrupted structural and functional connectivity of prefrontal–limbic structures and impaired emotional, cognitive, and autonomic responses. Detection of this injury before combat would have been unlikely in a clinical context because our veteran had reported a phenomenologically mild injury, and PTSD is a simple explanation for substance abuse, sleep impairment, and psychosocial distress. However, it may be that right prefrontal–limbic disruption imparted vulnerability for the development of PTSD and exacerbated our veteran’s emotional response to, and recovery from, PTSD.
Pathogenic LRRK2 regulates ciliation probability upstream of tau tubulin kinase 2 via Rab10 and RILPL1 proteins

Significance Mutations that activate LRRK2 protein kinase cause Parkinson's disease. LRRK2 phosphorylates a subset of Rab GTPases, in particular Rab8 and Rab10. We show here details related to the mechanism by which Rab10 phosphorylation blocks initiation of cilia formation, fundamental information related to how pathogenic LRRK2 interferes with normal cell physiology. Mutations that activate LRRK2 protein kinase cause Parkinson's disease. We showed previously that Rab10 phosphorylation by LRRK2 enhances its binding to RILPL1, and together, these proteins block cilia formation in a variety of cell types, including patient derived iPS cells. We have used live-cell fluorescence microscopy to identify, more precisely, the effect of LRRK2 kinase activity on both the formation of cilia triggered by serum starvation and the loss of cilia seen upon serum readdition. LRRK2 activity decreases the overall probability of ciliation without changing the rates of cilia formation in R1441C LRRK2 MEF cells. Cilia loss in these cells is accompanied by ciliary decapitation, and kinase activity does not change the timing or frequency of decapitation or the rate of cilia loss but increases the percent of cilia that are lost upon serum addition. LRRK2 activity, or overexpression of RILPL1 protein, blocks release of CP110 from the mother centriole, a step normally required for early ciliogenesis; LRRK2 blockade of CP110 uncapping requires Rab10 and RILPL1 proteins and is due to failure to recruit TTBK2, a kinase needed for CP110 release. In contrast, deciliation probability does not change in cells lacking Rab10 or RILPL1 and relies on a distinct LRRK2 pathway. These experiments provide critical detail to our understanding of the cellular consequences of pathogenic LRRK2 mutation and indicate that LRRK2 blocks ciliogenesis upstream of TTBK2 and enhances the deciliation process in response to serum addition.
Conversion of Racemic Unnatural Amino Acids to Optically Pure Forms by a Coupled Enzymatic Reaction

Genetic code expansion (GCE) technology is a useful tool for the site-specific modification of proteins. An unnatural amino acid (UAA) is one of the essential components of this technique, typically required at high concentration (1 mM or higher) in growth medium. The supply of UAAs is an important limitation to the application of GCE technology, as many UAAs are either expansive or commercially unavailable. In this study, two UAAs in a racemic mixture were converted into optically pure forms using two enzymes, the d-amino acid oxidase (RgDAAO) from Rhodotorula gracilis and the aminotransferase (TtAT) from Thermus thermophilus. In the coupled enzyme system, RgDAAO oxidizes the d-form of UAAs in a stereospecific manner and produces the corresponding α-keto acids, which are then converted into the l-form of UAAs by TtAT, resulting in the quantitative and stereospecific conversion of racemic UAAs to optically pure forms. The genetic incorporation of the optically pure UAAs into a target protein produced a better protein yield than the same experiments using the racemic mixtures of the UAAs. This method could not only be used for the preparation of optically pure UAAs from racemic mixtures, but also the broad substrate specificity of both enzymes would allow for its expansion to structurally diverse UAAs.
Individual and Joint Associations of Genetic Risk and Healthy Lifestyle Score with Colorectal Neoplasms Among Participants of Screening Colonoscopy

Genetic factors have strong impact on the risk of colorectal neoplasms, which may be reduced by healthy lifestyle. Similarly strong associations in relative terms across all levels of genetic risk imply that a healthy lifestylemay be beneficial due to higher absolute risk reduction in those at highest genetic risk. Genetic and lifestyle factors contribute to colorectal cancer risk. We investigated their individual and joint associations with various stages of colorectal carcinogenesis. We assessed associations of a polygenic risk score (PRS) and a healthy lifestyle score (HLS) with presence of nonadvanced adenomas and advanced neoplasms among 2,585 participants of screening colonoscopy from Germany. The PRS and HLS individually showed only weak associations with presence of nonadvanced adenomas; stronger associations were observed with advanced neoplasms (ORs, 95% CI, for highest vs. lowest risk tertile: PRS 2.27, 1.78–2.88; HLS 1.96, 1.53–2.51). The PRS was associated with higher odds of advanced neoplasms among carriers of any neoplasms (1.65, 1.23–2.22). Subjects in the highest risk tertile (vs. lowest tertile) of both scores had higher risks for nonadvanced adenomas (1.77, 1.09–2.86), for advanced neoplasms (3.95, 2.53–6.16) and, among carriers of any neoplasms, for advanced versus nonadvanced neoplasms (2.26, 1.31–3.92). Both scores were individually associated with increased risk of nonadvanced adenomas and, much more pronounced, advanced neoplasms. The similarly strong association in relative terms across all levels of genetic risk implies that a healthy lifestyle may be particularly beneficial in those at highest genetic risk, given that the same relative risk reduction in this group would imply a stronger absolute risk reduction. Genetic factors may be of particular relevance for the transition of nonadvanced to advanced adenomas. Prevention Relevance: Genetic factors have strong impact on the risk of colorectal neoplasms, which may be reduced by healthy lifestyle. Similarly strong associations in relative terms across all levels of genetic risk imply that a healthy lifestyle may be beneficial due to higher absolute risk reduction in those at highest genetic risk.
Understanding the impact of professional motivation on the workforce crisis in medicine: a rapid review

Background The NHS is facing a workforce crisis. Responses to date have focused on improving recruitment of staff, but less attention has been paid to retention. Aim To conduct a rapid review using Rosabeth Moss Kanter’s three Ms model of workforce motivation as a sensitising framework to examine the current medical workforce crisis. The work considers how insights from research in other professions offers new thinking for understanding what motivates doctors to continue working. Design & setting Rapid literature review with secondary analysis of existing research examining reasons for leaving medicine. Method A systematic search strategy was developed with the aid of an information specialist. The search terms used were: medical professionals, retention, and NHS. The exclusions were: commentaries, non-medical professionals, non-English language, and it was limited to post-1990. The search was applied to three electronic databases, MEDLINE, Embase, and Healthcare Management Information Consortium (HMIC). This produced a dataset describing study design, and factors related to motivation for leaving the medical profession. Comparative thematic analysis distilled core themes explaining the reasons for leaving and their relation to the three Ms model. Results Of 3389 abstracts identified, screening and assessment produced 82 articles included in the final analysis. Thematic analysis identified four key themes: low morale, disconnect, unmanageable change, and lack of personal and professional support. The themes of mastery, membership, and meaning were substantially present within the dataset. Conclusion Kanter's three Ms model of motivation can be applied to the medical workforce to understand retention issues. This work supports the development of targeted solutions to tackle the worsening workforce crisis.
Studies in catalytic steam gasification of petroleum coke with special reference to the effect of particle size

The object of this work is to study the effect of particle size, porosity, and thickness of a pellet on the gasification of very low ash (<0.5%) petroleum coke with steam both in the presence and absence of catalyst. Isothermal runs were carried out in the temperature range 695-880/sup 0/C using different sizes of particles (0.037-0.59 mm) and also different thicknesses of flat plates (0-7 mm) prepared from these particles. The experimental results showed some unusual behavior. The rate constant was observed to increase with an increase in particle size as well as with a decrease in thickness of the flat plates.
Complications of reverse shoulder arthroplasty: a concise review

Reverse shoulder arthroplasty is an ideal treatment for glenohumeral dysfunction due to cuff tear arthropathy. As the number of patients treated with reverse shoulder arthroplasty is increasing, the incidence of complications after this procedure also is increasing. The rate of complications in reverse shoulder arthroplasty was reported to be 15%–24%. Recently, the following complications have been reported in order of frequency: periprosthetic infection, dislocation, periprosthetic fracture, neurologic injury, scapular notching, acromion or scapular spine fracture, and aseptic loosening of prosthesis. However, the overall complication rate has varied across studies because of different prosthesis used, improvement of implant and surgical skills, and different definitions of complications. Some authors included complications that affect the clinical outcomes of the surgery, while others reported minor complications that do not affect the clinical outcomes such as minor reversible neurologic deficit or minimal scapular notching. This review article summarizes the processes related to diagnosis and treatment of complications after reverse shoulder arthroplasty with the aim of helping clinicians reduce complications and perform appropriate procedures if/when complications occur.
Tinnitus and associations with chronic pain: The population-based Tromsø Study (2015–2016)

Tinnitus and pain have many similarities. Both are subjective sensations that may turn chronic, they are often accompanied by hypersensitivity in their respective sensory system, and overlapping brain changes have been observed. Since no population study has examined the empirical association between chronic pain and tinnitus, the present study aimed to explore the relationship in a general adult population. We used data from the seventh survey of the Tromsø Study (2015–2016). Participants (aged ≥40) responded to questions about pain and tinnitus. Using multiple logistic regression, we analysed the adjusted relationship between chronic pain and tinnitus in the full sample (n = 19,039), using several tinnitus definitions ranging from tinnitus >5 minutes within the past 12 months (broadest definition) to at least weekly and highly bothersome tinnitus (strictest definition). We also analysed relationships between number of body regions with pain, pain intensity and bothering, and tinnitus >5 minutes, among participants with chronic pain (n = 11,589). We found an association between chronic pain and tinnitus that was present irrespective of tinnitus definition, but was stronger with more bothersome tinnitus. With chronic pain, the odds of tinnitus >5 minutes was 64% higher, while odds of at least weekly, highly bothersome tinnitus was 144% higher than without chronic pain. Among participants with chronic pain, the number of pain regions was the pain variable most strongly associated with tinnitus >5 minutes (OR = 1.17 (95% CI: 1.14–1.20) for an increase of one region), whereas the other pain variables (intensity and bothering) showed weaker associations. All chronic pain variables had significant interactions with age, with the strongest associations for the youngest individuals (40–54 years). Our findings support the existence of an association between chronic pain and tinnitus and emphasises the importance of examining for comorbid pain in tinnitus patients to provide a more comprehensive treatment of tinnitus.
The effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on ischemic stroke and the possible underlying mechanisms

Abstract Purpose: As of November 28, 2020, COVID-19 has been reported in 220 countries with 61,036,793 confirmed cases and 1,433,316 confirmed deaths; countries became vigilant around the world. In addition to SARS-CoV-2 causing pneumonia, many studies have reported ischemic stroke in patients with COVID-19. This article describes the effects and possible underlying mechanisms of SARS-CoV-2 on ischemic stroke. Materials and methods: A literature search was performed using PubMed, Web of Science, and other COVID-dedicated databases and the combination of the keywords ‘SARS-CoV-2’, ‘COVID-19’ and ‘ischemic stroke’ up to November 28, 2020. Results: SARS-CoV-2 invades the host through angiotensin converting enzyme 2 (ACE2). ACE2 is expressed not only in the lungs, but also in the brain and vascular endothelial cells. SARS-CoV-2 infection might cause direct vascular disease or enhance the immunogenic thrombosis environment through several mechanisms. SARS-CoV-2 infection can modulate the host immune response and can cause inflammation, coagulation disorders, renin angiotensin system disorders, hypoxia, and stress disorders, which may lead to the occurrence of ischemic stroke. Conclusions: Some patients with COVID-19 can develop ischemic stroke. Ischemic stroke has a high risk of causing disability and is associated with a high mortality rate. It is hoped that when medical staff treat patients with COVID-19, they would pay attention to the occurrence of ischemic stroke to improve the prognosis of patients with COVID-19.
Assessment of toxic effects of pesticide extracts on different green algal species by using chlorophyll a fluorescence

In this study, the toxicity of pesticides extracted from the Yamaska River watershed surface waters were tested on three species of green algae (Chlorella vulgaris, Scenedesmus obliquus and Pseudokirchneriella subcapitata) by using slow and fast fluorescence kinetics (Maxi-Imaging-Pulse-Amplitude-Modulated and Plant Efficiency Analysis). Two types of extractions for a variety of herbicides and insecticides (organosphosphorous-type (OP) and phenoxyacid-type (PA)) were conducted on the water samples of three sites having different pesticide concentrations (‘Deborah Stairs’, ‘Rivière Noire’ and ‘Rivière à la Barbue’). Our results showed that of the three species of green algae studied, S. obliquus was the most sensitive to both pesticide extracts. Moreover, PA pesticide extracts from ‘Rivière à la Barbue’ were the most toxic since the algae exposed to those extracts had the lowest photosynthetic activity when evaluated by the PSII quantum yields (ΦM and ), the photochemical quenching (qP(REL)) and the electron transport rate per reaction center (ET0/RC). The fast fluorescence kinetic data also indicate that the site of action of the different extracts was at the level of the primary photochemical events, probably electron flow blockage at the Q B binding site. This study demonstrated the advantages of using chlorophyll fluorometry in assessing toxic effect of pesticide mixtures.