Miracle-dz/UniFAR-FTUs huggingface dataset:
Rac1 Promotes Cell Motility by Controlling Cell Mechanics in Human Glioblastoma

The failure of existing therapies in treating human glioblastoma (GBM) mostly is due to the ability of GBM to infiltrate into healthy regions of the brain; however, the relationship between cell motility and cell mechanics is not well understood. Here, we used atomic force microscopy (AFM), live-cell imaging, and biochemical tools to study the connection between motility and mechanics in human GBM cells. It was found thatRac1 inactivation by genomic silencing and inhibition with EHT 1864 reduced cell motility, inhibited cell ruffles, and disrupted the dynamics of cytoskeleton organization and cell adhesion. These changes were correlated with abnormal localization of myosin IIa and a rapid suppression of the phosphorylation of Erk1/2. At the same time, AFM measurements of the GBM cells revealed a significant increase in cell elasticity and viscosity following Rac1 inhibition. These results indicate that mechanical properties profoundly affect cell motility and may play an important role in the infiltration of GBM. It is conceivable that the mechanical characters might be used as markers for further surgical and therapeutical interventions.
Xanthophyll Cycle and Its Relative Enzymes

Light is a fundamental source of energy but is also potentially harmful to organisms. Plants have evolved a variety of regulatory mechanisms to respond to the naturally varying light conditions. Xanthophyll cycle is now recognized as a key regulator and photoprotective mechanism found ubiquitously in plants. Xanthophyll cycle has multiple functions, such as thermal dissipation, protection against oxidative stress caused by light, modulation of the structure of thylakoid membrane, involving in blue light signal transduction and regulating the synthesis of ABA (Abscisic acid). VDE (Violaxanth de-epoxidase) and ZE (zeaxanth epoxidase), are involved in xanthophyll cycle. This paper outlined the functions of xanthophylls cycle and its relative enzymes. Key word: Xanthophyll cycle, function, VDE, ZE.
THE EFFECTS OF SHORT AND MIDDLE TIMES AEROBIC EXERCISE WITH HIGH INTENSITIES ON INGREDIENTS ANTIOXIDANT IN FEMALE SPRAGUE DAWLEY RATS

Introduction: Different physical activities have an effects on antioxidant system. Variety, period and intensity of activities are necessary in improvement of safety in society. The purpose of this study was to evaluate the effect of high intensity training with consumption of coenzyme Q10 (CoQ10) on antioxidant enzymes activities in female Sprague Dawley rats. Methods: A total of 50 female Sprague dawley rats were divided into 4 groups: control, CoQ10, CoQ10 + exercise and exercise. Some groups were supplemented with CoQ10 (20 mg/kg) and the others were training with exercise programs for 4 weeks. The exercise programs were performed 5 times in a week with speed (10-17 m/s) in slope range (5< slope < 15) for 15-60 minutes. After that, Blood samples were drawn from heart and Enzyme activities of SOD and CAT were measured just before, after training and 24 hours later. The activities of the enzymes were determined by using enzyme linked immune sorbent assay kit (ELISA). Data were analyzed using one-way ANOVA test. Result: The results showed that high intensity training and CoQ10 had significant effects on SOD enzyme activity in groups of rats which trained with CoQ10+ exercise or exercise. Also, average CAT enzyme activity in rats which received CoQ10, showed a significant increase compared with other groups. Conclusion: It seems that using of CoQ10 with doing regulatory aerobic exercise after training and 24 hours later can cause adaptation in antioxidant enzyme system. However, CAT activity was decreased.
Implementing harvest strategies in Australia: 5 years on

Australian Commonwealth fisheries are managed using a formal harvest strategy policy (HSP) introduced by the federal government in 2007. At the State level, a number of commercial fisheries are also managed under formal harvest strategies, but no overarching policy currently exists to guide their consistent implementation across jurisdictions. There have been 5 years of experience with implementation of the Commonwealth policy across the highly diverse array of commercial fisheries found in Australia. The HSP has an explicit target of maximum economic yield, and an explicit limit set at half the biomass that would support maximum sustainable yield. The policy also specifies an acceptable level of risk associated with falling below the limit reference point. We discuss the experience gained from implementing the HSP in Australia, including a number of challenges faced, and attempt to summarize the benefits and costs of implementing harvest strategies. Our view is that, overall, the benefits clearly outweigh the costs.
An Examination of a Support Tool for Designing Shadow Box Art

We examine a system that supports creating shadow box art works. The creators of shadow box art carefully determine the number of layers and their shapes to cut out, considering how shadows fall and the thickness of the frame. We investigate the crafting process and design our prototype functions: (1) a user interface for designing shadow box art, (2) a simple viewer considering shadows using 3DCG, and, (3) reducing paper footprint by rearranging cut-out parts. Through creating actual artworks, we confirm that our system enables the user to make layer design smoothly and the creation of shadow box art relatively easily.
Differentiating Breast Myopathies through Color and Texture Analyses in Broiler

Wooden breast (WB), white striping (WS) and spaghetti meat (SM) are breast myopathies of the Pectoralis major that greatly affect meat quality in broilers. To differentiate color and texture characteristics with instrumental methods, some of them applied for the first time in this species, 300 carcasses were randomly chosen from an abattoir from five different flocks from the same farm, at a rate of 60 carcasses from each flock. Twenty-four hours after slaughter, both side breasts were dissected, and yields calculated. Color was measured on the surface of the breast with a spectrocolorimeter and reflectance values obtained. Texture was measured on raw meat with a modified compression test that hinders the fiber from expanding transversally and a texture profile analysis (TPA) and also on cooked meat with a Warner–Bratzler shear and a TPA. Color differs between severity degrees, increasing redness (from −1.77 to −1.32 in WB) and, especially, yellowness (from 5.00 to 6.73 in WS) and chroma (from 5.75 to 7.22 in SM) with the severity of the myopathy. The subtraction R630 minus R580 was found to be a useful index to differentiate breast myopathies degrees. The modified compression test can be considered an effective tool to assess the hardness of different structures in each myopathy. Texture differences in the myopathies are better assessed in raw than in cooked meat.
Effects of a Third Party on Rapport in Investigative Interviews

This research examined how the introduction of a third party impacts interviewee rapport in an investigative setting. It has been speculated that a “third person in the communications loop” during an investigative interview may negatively impact critical components of the information collection process, including the establishment of rapport. This exploratory research adopted an innovative content analytic approach using the Linguistic Inquiry and Word Count (LIWC) to examining the construct of rapport, a core aspect of the interview process. Based on the results of this study, rapport, as conceptualized by the three-component model of mutual attentiveness, positivity, and coordination, was not negatively impacted by the presence of a third party in an investigative context. This is practically important because many law enforcement interviews use multiple interviewers and this suggests that this practice will not have a negative effect on rapport in the interview. Additionally, the findings indicate that the anecdotal concern that “three is a crowd” and that the addition of a third party may impact rapport is unfounded.
Asymmetric lenticulostriate arteries in patients with moyamoya disease presenting with movement disorder: three new cases

ABSTRACT Objective Unilateral movement disorder associated with moyamoya disease is a rare finding and the mechanism remains to be fully elucidated. Theories postulated include contralateral cerebral ischemic or hemorrhagic lesions, and/or hypoperfusion. However, few studies have reported such patients without contralateral lesions nor hypoperfusion. This study aimed to explore the potential mechanism of those who had neither contralateral cerebral lesions nor hypoperfusion. Methods Neuroradiological features of lenticulostriate arteries in three cases with unilateral movement disorder associated with moyamoya disease who had neither contralateral lesions nor hypoperfusion were mainly analyzed. Results Angiography and 3 T black-blood T1-weighted intracranial vessel wall imaging both demonstrated a significant asymmetry between bilateral lenticulostriate arteries qualitatively and quantitatively on admission. After one-year follow-up, two patients’ vessel wall imaging indicated that the asymmetry diminished, and the symptoms spontaneously resolved. Conclusion This report demonstrated that patients with moyamoya disease with unilateral movement disorder who had neither contralateral lesions nor hypoperfusion may be related to the asymmetry between bilateral lenticulostriate arteries through basal ganglia.
Desempenho agronômico do milho para produção de silagem, cultivado em sucessão a plantas de cobertura

Agronomic performance of corn for silage grown after cover crops This study aimed to evaluate the agronomic performance of corn for silage, grown after cover crops. The experimental design was a randomized block design with four replications and seven treatments. The treatments were corn grown in the straw of 1) oat; 2) oat + oilseed radish; 3) oat + white lupine; 4) white lupine; 5) crambe; 6) spontaneous vegetation (fallow ground) and 7) spontaneous vegetation (fallow ground) + nitrogen fertilizer in the V6 phase in the amount of 100 kg ha -1 of N. It was evaluated: plant height, ear placement height, stalk diameter below and above the ear placement, number of leaves below and above the ear placement, ear diameter and length, ear count and yield of green mass (biomass). Corn plants were harvested between stages R5. Biomass production of maize grown under different straw covering plants is not influenced significantly, regardless of the species used. The application of nitrogen did not influence the production of biomass for silage.
Behaviour of Dunaliella Salina in microfluidic contraction devices

The importance of microorganisms and their fundamental functions in different contexts is well-documented; they constitute a major part of the world's biomass and play a vital biological role in ecosystems. Microorganisms can be found in almost every part of the biosphere and are utilised in a wide range of applications that encompass food and beverage preparation, genetic engineering and renewable energy. [1, 2]
Global Burden of Thyroid Cancer From 1990 to 2017

Key Points Question What were the epidemiologic patterns and variation in the trends of thyroid cancer worldwide from 1990 to 2017? Findings In this cross-sectional study covering data on incidence, deaths, and disability-adjusted life-years and their temporal trends from 195 countries and 21 regions, increasing trends of thyroid cancer burden were observed, with significant differences by sex, region, country, age, and sociodemographic index. Almost half of the thyroid cancer burden was noted in Southern and Eastern Asia, and a third of patients with thyroid cancer resided in countries with a high sociodemographic index. Meaning This study suggests an increasing global burden of thyroid cancer; the geographic disparities may provide support for cancer health care planning and resource allocation.
Dual Relations Network for Collaborative Filtering

Collaborative filtering (CF) is one of the most effective and popular recommendation methods. Deep learning has achieved great success in various tasks and extensive work has applied deep learning into collaborative filtering. However, existing deep learning based CF methods only capture either user-item relations (users’ affection for target items) or item-item relations (association between target items and users’ historical items), which may lead to sub-optimal recommendation accuracy. In this paper, we propose deep dual relations network, a neural network based approach that can model user-item relations and item-item relations simultaneously. Furthermore, considering the fact that a historical item a user prefers will have a more positive impact on his decision on a new item, user-item relations should play an important role in item-item relations modeling, which is not investigated by previous work. To solve this problem, we propose a special architecture, named affection-based attention network to capture the impact of user-item relations on item-item relations modeling. Comprehensive experimental results on four real-world datasets demonstrate the effectiveness of our proposed model.
Simulation & Analysis of Protection for Power System Networks by Non Super Conducting Fault Current Limiter

Fault current protection is a major issues in power quality. To mitigatethe increasing fault current issues, various techniques had been developed. The most straight forward way would be upgrading all the conductors, switchgear and protection devices in existing power systems to raise their fault current ratings and interrupting speed but this is expensive. Another is to replace all damage equipment, which is complicated and time consuming. Fault current level is confined to lower level of acceptable limit by using Fault Current Limiters (FCLs). This paper utilizes Non-Super Conducting Fault Current Limiter (NSFCL) with a control strategy to maintain the fault current. This gives an added advantage of improved voltage profile in faulty phase. The proposed technique, NSFCL has been studied and simulated in virtual environment provided by MATLAB Simulink.
Quantitative Evaluation of Vehicle Seat Driving Comfort During Short and Long Term Driving

Regarding changes of subjective and objective parameters of comfort during short and long-term driving, the arbitrariness of traditional subjective evaluation methods, as well as the defects of objective method where issues can be qualitatively but not quantitatively analyzed. By measuring pressure distribution and electromyography during short and long-term driving respectively, the article obtained pressure indicators, electromyography characteristic parameters and the corresponding subjective evaluations of drivers. The variation of subjective and objective parameters of comfort, the difference in terms of comfort and the fatigable body parts were tested. By means of correlation analysis completed the indicators screen. Proposed a new comprehensive weighting method-AHP to limit entropy method, established the mapping relations between subjective comfort and objective indicators, which based on pressure distribution and physiological information during short-term and long-term driving. Obtained a quantitative evaluation method that applies pressure distribution, physiological information and subjective evaluation to effectively evaluate driving comfort, and thus provided a theoretical basis for evaluating driving comfort.
Contribution of Monocarboxylate Transporter 6 to the Pharmacokinetics and Pharmacodynamics of Bumetanide in Mice

Bumetanide, a sulfamyl loop diuretic, is used for the treatment of edema in association with congestive heart failure. Being a polar, anionic compound at physiologic pH, bumetanide uptake and efflux into different tissues is largely transporter-mediated. Of note, organic anion transporters (SLC22A) have been extensively studied in terms of their importance in transporting bumetanide to its primary site of action in the kidney. The contribution of one of the less-studied bumetanide transporters, monocarboxylate transporter 6 (MCT6; SLC16A5), to bumetanide pharmacokinetics (PK) and pharmacodynamics (PD) has yet to be characterized. The affinity of bumetanide for murine Mct6 was evaluated using Mct6-transfected Xenopus laevis oocytes. Furthermore, bumetanide was intravenously and orally administered to wild-type mice (Mct6+/+) and homozygous Mct6 knockout mice (Mct6−/−) to elucidate the contribution of Mct6 to bumetanide PK/PD in vivo. We demonstrated that murine Mct6 transports bumetanide at a similar affinity compared with human MCT6 (78 and 84 μM, respectively, at pH 7.4). After bumetanide administration, there were no significant differences in plasma PK. Additionally, diuresis was significantly decreased by ∼55% after intravenous bumetanide administration in Mct6−/− mice. Kidney cortex concentrations of bumetanide were decreased, suggesting decreased Mct6-mediated bumetanide transport to its site of action in the kidney. Overall, these results suggest that Mct6 does not play a major role in the plasma PK of bumetanide in mice; however, it significantly contributes to bumetanide’s pharmacodynamics due to changes in kidney concentrations. SIGNIFICANCE STATEMENT Previous evidence suggested that MCT6 transports bumetanide in vitro; however, no studies to date have evaluated the in vivo contribution of this transporter. In vitro studies indicated that mouse and human MCT6 transport bumetanide with similar affinities. Using Mct6 knockout mice, we demonstrated that murine Mct6 does not play a major role in the plasma pharmacokinetics of bumetanide; however, the pharmacodynamic effect of diuresis was attenuated in the knockout mice, likely because of the decreased bumetanide concentrations in the kidney.
CYTOGENETIC COMPARISON OF SOME PINNIPEDS (MAMMALIA: EUTHERIA)

Karyotypes of the harbor seal, Phoca vitulina (2N = 32), the bearded seal, Erignathus barbatus (2N = 34), the Weddell seal, Leptonychotes weddelli (2N = 34), the walrus, Odobenus rosmarus (2N = 32), the Steller sea lion, Eumetopias jubata (2N = 36), and the northern fur seal, Callorhinus ursinus (2N = 36), are described and compared with those of the ringed seal, Pusa hispida, and California sea lion, Zalophus californianus, reported by other workers. These are discussed with reference to current theories of pinniped phylogeny and systematics. The number and morphology of chromosomes in these eight species, representing each family and subfamily of pinnipeds, were found to be remarkably similar. This is interpreted as evidence of close interrelationship, commensurate with the theory of monophyletic origin. Karyograms of the harbor seal and ringed seal are virtually identical, as are those of the bearded and Weddell seals and those of the Steller and California sea lions. The karyotype of the fur seal is s.
Teleconsultation at a tertiary care government medical university during COVID-19 Lockdown in India – A pilot study

Purpose: COVID-19 related pan- India lockdown brought teleophthalmology to the forefront. The study ventures to understand the relevance of this modality in a government setup. The objective is to understand the feasibility, clinical profile and addressability of patients using teleconsultation in ophthalmology at a tertiary care government medical university during the COVID-19 Lockdown in India. Methods: An online survey targeting faculty members and resident doctors in a tertiary eye center in a government medical university in north India was conducted. Various aspects of teleconsultation were analyzed including the number and preferential mode of consultations, commonest complaints and diagnoses made. Frequency and factors mandating physical examination of patients was also analyzed. Results: The questionnaire was sent to 40 ophthalmologists of whom 38 responded. A total of 4880 teleconsultations were given. The commonest mode of communication was by WhatsApp messages (65.6%) and E-mail was the least preferred medium. More than 80% consultations were from previously seen patients. Red eye was the commonest presenting complaint (22.8%), followed by watering (18.7%) and foreign body sensation (14.5%). Computer vision syndrome was the commonest diagnosis (25.9%) followed by conjunctivitis (17.7%) and refractive error (17.7%). About 40% required physical examination, mostly due to uncertain diagnosis (22%) or inadequate response to prescribed treatment (19%). Conclusion: Teleconsultation was feasible in a government medical university for providing ophthalmic services during lockdown. WhatsApp was the preferred communication modality, computer vision syndrome was the most frequent tentative diagnosis and approximately 60% did not require in-person physical examination.
Multiple Salmonella-pathogenicity island 2 effectors are required to facilitate bacterial establishment of its intracellular niche and virulence

The pathogenesis of Salmonella Typhimurium depends on the bacterium’s ability to survive and replicate within host cells. The formation and maintenance of a unique membrane-bound compartment, termed the Salmonella-containing vacuole (SCV), is essential for S. Typhimurium pathogenesis. SCV-bound S. Typhimurium induces formation of filamentous tubules that radiate outwards from the SCV, termed Salmonella-induced filaments (SIFs). SIF formation is concomitant with the onset of replication within host epithelial cells. SIF biogenesis, formation and maintenance of the SCV, and the intracellular positioning of the SCV within the host cell requires translocation of bacterial proteins (effectors) into the host cell. Effectors secreted by the type III secretion system encoded on Salmonella pathogenicity island 2 (T3SS2) function to interfere with host cellular processes and promote both intracellular survival and replication of S. Typhimurium. Seven T3SS2-secreted effectors, SifA, SopD2, PipB2, SteA, SseJ, SseF, and SseG have previously been implicated to play complementary, redundant, and/or antagonistic roles with respect to SIF biogenesis, intracellular positioning of the SCV, and SCV membrane dynamics modulation during infection. We undertook a systematic study to delineate the contribution of each effector to these processes by (i) deleting all seven of these effectors in a single S. Typhimurium strain; and (ii) deleting combinations of multiple effectors based on putative effector function. Using this deletion mutant library, we show that each of SIF biogenesis, intracellular SCV localization, intramacrophage replication, colonization, and virulence depends on the activities of multiple effectors. Together, our data demonstrates the complex interplay between these seven effectors and highlights the necessity to study T3SS2-secreted effectors as groups, rather than studies of individual effectors.