Miracle-dz/UniFAR-FTUs huggingface dataset:
Hematopoietic growth factor expression and ATRA sensitivity in acute promyelocytic blast cells

Acute promyelocytic leukemia (APL) is a homogeneous subgroup of acute myeloid leukemias (AMLs) characterized by the presence of the t(15.17) translocation and the resulting promyelocytic myeloid leukemia/retinoic acid receptor a (PML/RARa) fusion proteins. To date APL is the only AML that is sufficiently sensitive to all-trans retinoic acid’s (ATRA) differentiating effect. In vivo ATRA alone achieves complete remission in most APL patients. However, failure or partial responses are observed and the molecular basis of the absence of ATRA response in these patients has not been determined. To gain insights in the cell growth and differentiation of APL cells, expression of hematopoietic growth factors (HGF) shown to be produced by leukemic cells (interleukinlp [IL-lp], IL-6, tumor necrosis factor a (TNFa), granulocyte colony-stimulating factor [G-CSF], granulocyte-macrophage CUTE MYELOID LEUKEMIA (AML) is a malignant process that results from the accumulation in the blood and bone marrow (BM) of myeloid precursor cells characterized by abnormal growth and differentiation arrest. Both autocrine and paracrine hypotheses have been brought forward to suggest possible mechanisms for the transformation and invasive process. AML cells have been shown to express the genes or produce a variety of hematopoietic growth factors (HGFs), including granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF),’ and cytokines such as interleukin-l (IL-l), IL6,2-5 and tumor necrosis factor cu (TNFcu). ’ Possible use of these factors by the AML cells themselves (GM-CSF, GCSF, IL-6, IL-I) or by accessory cells (TNFcu, IL-l) may be responsible for both the autocrine/paracrine growth stimulation of the AML cell^,'^*^^^^ and the induction of leukemic cell differentiation. ’ Little data concerning the expression and secretion pattern of growth factors by acute promyelocytic leukemia (APL) cells have been reported in the literature. APL, the AML3 French-American-British (FAB) classification subtype,R is a rare subgroup of AML (less than 10%) characterized by
Performance loss of Corynebacterium glutamicum cultivations under scale‐down conditions using complex media

Substrate and oxygen gradients appear in industrial‐scale fed‐batch processes due to limitations in the achievable power input and concomitantly increased mixing times. In order to mimic these gradients at lab scale, scale‐down reactors are applied. Previous studies in such reactor systems suggest that Corynebacterium glutamicum is robust against oscillatory oxygen and substrate availability in relation to growth and side product accumulation. Usually, defined mineral salt media are applied contrary to the industrial case, in which complex media containing different carbon sources are used. Therefore, this study investigated the cultivation performance using complex medium based on sucrose, molasses, and corn steep liquor in a three‐compartment scale‐down reactor. The reactor consisted of a stirred tank and two plug flow reactor modules. This approach was applied based on assumptions of gradient distributions in bottom‐fed bioreactors. A drastic reduction of growth and volumetric product yield of a cadaverine producing strain was observed while several short chain fatty acids accumulated, among them l‐lactate and acetate. Growth was depleted after several hours of cultivation, while the substrate uptake rate was reduced by 20%. Hence, the main carbon source sucrose accumulated after 10 h of fed‐batch cultivation. Despite growth cessation, neither reduction of cell vitality nor increased cell lysis were observed.
Estudio de las infecciones por el hongo ichthyophonus sp. En peces de interes comercial

En esta tesis se ha realizado un estudio de las caracteristicas morfologicas, fisiologicas y bioquimicas de ichthyophonus sp. Y un estudio de la epidemiologia, evolucion y patologia de la ictiofonosis en diversos hospedadores. Tambien se realizo un ensayo de tratamiento con ketoconazol. La fase de ichthyophonus sp. Que predomina en los tejidos de los diferentes hospedadores estudiados es el conidio multinucleado de pared gruesa que evoluciona segun las condiciones del medio en el que se encuentra. Este hongo puede ser cultivado en distintos medios, pero los mejores resultados se obtuvieron con mem con un 1% de l-glutamina, un 10% de suero fetal bovino, 0,5% de cloruro sodico, ph neutro y una ta de incubacion de 14 c. Ichthyophonus sp. Es un hongo con un amplio espectro de hospedadores y distribucion geografica en nuestro pais. La prevalencia de infeccion esta relacionada con la edad de los peces tanto en rodaballo, como en doradas y lubinas. En todas las especies estudiadas la respuesta frente a este hongo es inflamatoria y se caracteriza por la formacion de granulomas que en lubina tienen una fuerte pigmentacion anaranjada. En el ensayo preliminar del tratamiento con ketoconazol no se obtuvieron resultados concluyentes. Sin embargo, los buenos resultados obtenidos con ketoconazol liposomado "in vitro", aconsejan la realizacion de nuevas pruebas con dosis mayores a las ensayadas.
Hairy cell interactions with extracellular matrix: expression of specific integrin receptors and their role in the cell's response to specific adhesive proteins

Integrin/extracellular-matrix interactions are central to the migration, localization, and subsequent function of lymphocytes within tissues. In hairy cell leukemia (HCL) the malignant cells display a highly characteristic tissue distribution in which interactions with extracellular matrix (ECM) are often prominent. Therefore, we used HCL as a model in which to investigate the poorly understood integrin/ECM interactions that underlie the migratory behavior of malignant B lymphocytes. Using a combined approach involving immunocytochemistry, flow cytometry, and immunoprecipitation analysis, hairy cells (HCs) were shown to have a consistent and distinctive phenotype (mainly alpha 4 beta 1, alpha 5 beta 1, alpha v beta 1, and alpha v beta 3). Furthermore, functional studies utilising adhesion assays, time-lapse video-microscopy and image analysis showed that the HCs displayed very specific adhesive behaviour in response to relevant adhesive protein ligands. HCs were able to adhere to different extents on all the adhesive proteins examined, but, on laminin and collagen, binding was weak with little cytoplasmic spreading. In contrast, the cells showed strong adhesion both to fibronectin (FN) and to vitronectin (VN). On FN, the cells spread extensively with nonpolarized cytoplasmic projections, whereas on VN cytoplasmic projections were markedly polarized. This polarized morphology was shown to reflect cell motility. Investigation of the role of individual integrin receptors in the cell movement response suggested that alpha v beta 3 is the major integrin responsible for this motile behavior. These results are discussed in relation to the limited previous data on leukemic and activated B-cell integrins, and we suggest that the HC integrins play a significant role in the characteristic behavior of HCs within tissues.
Vers un élargissement des indications des antidépresseurs

Résumé Les données neurochimiques directes ou indirectes obtenues dans différentes affections du système nerveux central (SNC) constituent la base rationnelle de I'utilisation des médicaments dans des indications précises. Les antidépresseurs (AD) tricycliques agissant sur les principales amines du SNC trouvent ainsi des applications thérapeutiques variées en dehors de la dépression. L'arrivée de nouveaux AD au mécanisme d'action, soit plus cibé (la sérotonine), soit différent (IMAO) pose de nouveau cette question des indications psychiatriques et neurologiques, qui ne peut être résolue que par des essais méthodologiquement irréprochables. Une telle approche évitera d'ignorer pendant des années des effets thérapeutiques incontestables d'une molécule ancienne, tel que ce fut le cas de la clomipramine dans les troubles obsessifs-compulsifs.
Do Nod-factor Levels Play a Role in Host-Specificity ?

Rhizobial Nod-factors are N-acylated oligomers of N-acetyl-D-glucosamine carrying a variety of substituents. At pico- to nano-molar concentrations, they provoke deformation and curling of nodulation competent root-hairs. Higher concentrations (10⁻⁷ to 10⁻⁶ M) elicit preinfection structures, allow Nod⁻ rhizobia to enter legume roots and to fix nitrogen within nodules. Evidence is accumulating that nodulation capacity is controlled both by specific Nod-factors as well as by Nod-factor concentrations. As an example, root-hairs of Macroptilium atropurpureum show identical "shepherd's crook" type curling when challenged with optimal (≈ 10⁻⁹ M) concentrations of Nod-factors of Bradyrhizobium japonicum and Rhizobium sp. NGR234 (which are Nod⁺) or with Nod-factors of R. leguminosarum and R. meliloti (both bacteria are incapable of nodulating Macroptilium). Similarly, conjugation of the nodD1 gene of NGR234 into R. meliloti allows nodulation of M. atropurpureum. We have used alkanisation of the tomato cell growth medium as a quantitative bioassay for Nod-factors. Upon induction with flavonoids, wild-type Rhizobium sp. NGR234 produces abundant Nod-factors, which may partly explain its extremely broad host-range. Nod-factors are produced throughout the log-phase of growth, while mutations in nodSU drastically reduce Nod-factor synthesis. Good correlations exist between the levels of Nod-factors and the ability to nodulate specific legumes.
EFFECT OF PAPRIKA EXTRACT SUPPLEMENT ON EGG YOLK COLOUR OF LAYER FED WHEAT-BASED DIETS

This experiment was conducted to test the effect of the paprika pigment ing additive on egg yolk colour of layer fed wheat-based diet.480 Lohman layers at 53 weeks were allotted to five dietary treatments randomly(three replicates o f 32 layers per replicate). The dietary treatments were:wheat diet(C),C+0.1%,C+0. 2%,C+0.4% and C+0.8% paprika extract,respectively.The results showed that:(1)The RCF of yolk in the trial groups increased significantly,the higher of paprika e xtract level,the more RCF was (P0.01). (2)With the increas of paprika extract le vel from 0 to 0.8%,a value increased quickly(P0.01),and L value decreased(P0.0 5),while b value had no significantly change(P0.05). (3)Yolks from the higher le vels of paprika extract showed a proportionally higher content of total caroteno id deposition,and there was significant difference among all levels(P0.01). Howo ver,there was no significant influence of paprika extract on egg quality and per formance of the layers.
Patterns of brown-headed cowbird parasitism in a recently invaded area and potential mechanisms limiting cowbird reproduction

of Dissertation Presented to the Graduate School of the University of Florida in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy PATTERNS OF BROWN-HEADED COWBIRD PARASITISM IN A RECENTLY INVADED AREA AND POTENTIAL MECHANISMS LIMITING COWBIRD REPRODUCTION By Matthew J. Reetz May 2008 Chair: Kathryn E. Sieving Cochair: Scott K. Robinson Major: Wildlife Ecology and Conservation Despite conservation implications surrounding recent Brown-headed Cowbird (Molothrus ater) expansion into the southeastern United States, there are few ecological data on cowbirds in Florida. Objectives for this project were to (1) quantify parasitism rates to determine the extent of brood parasitism and identity of host species and (2) test hypotheses explaining patterns of parasitism and cowbird distribution in Florida. I surveyed parasitism of 35 host species breeding in Florida through nest monitoring and observations of family groups with fledglings. Although cowbirds are moderately abundant in the study area, only 1.6% of 1117 nests and 6.1% of 278 family groups with fledglings were parasitized. While rates of parasitism are extremely low across the community and for individual hosts, my results suggest that cowbirds may preferentially parasitize a few host species nesting in the midstory and canopy. To help explain observed low cowbird parasitism rates, I tested egg rejection behavior of four common Florida songbird species known to reject parasitism elsewhere. I experimentally parasitized 78 nests using eggs obtained from a captive cowbird colony. Three of four species
Regular ArticleSexual selection and the temporal separation of reproductive events: sperm storage data from reptiles, birds and mammals

The duration of sperm storage by females differs markedly between reptiles (maximum: 2555 d [7 years] and birds (maximum: 117 d), with mammals showing both very short (< 1 d) and relatively long periods (maximum: 198 days). The females of many reptiles, probably all birds and some mammals have specialized structures for storing sperm, suggesting that selection for sperm storage has operated on females. Sperm storage, together with delayed implantation and delayed development, separates copulation from fertilization, and hence, copulation from the timing of birth. All three types of separation mechanism occur in mammals. Delayed implantation cannot occur in reptiles and birds because their eggs do not implant, but delayed development occurs in some reptiles. Comparisons among vertebrate classes suggest that sperm storage in the female tract may have an upper limit of a little over 100 days in endotherms: non-hibernating mammals have circumvented this limit by using delayed implantation as an uncoupling mechanism. A long reproductive cycle (up to four years) probably makes sperm storage obligatory for some reptiles. We consider existing hypotheses for prolonged sperm storage and the other reproductive delays, and propose a new hypothesis. Our hypothesis extends Sandell's (1190) hypothesis which states that in mammals delayed implantation has evolved to allow females to time both their copulation and birth seasons optimally. We propose that the other separation mechanisms, namely sperm storage and delayed development, have also evolved for this reason. Sandell suggested that the optimal time for females to copulate is when the opportunities to obtain the best quality male exist, and thus the dislocation of copulation and birth seasons has occurred through sexual selection. We propose that the other two separation mechanisms may also have evolved through sexual selection, in part at least. Of the three separation mechanisms, sperm storage has additional advantages for females in that it also allows them to modify their choice of male after copulation has occurred, through sperm competition. Thus we propose that: (1) all separation mechanisms have evolved when the optimal time for copulation is not compatible with either the optimal time for fertilization (birds) or the optimal time for birth, given a constant gestation period (reptiles, birds and mammals); (2) separation mechanisms have evolved through sexual selection enabling females to improve the quality of the male that fertilizes their eggs, either through pre-copulatory male-male competition (via any of the separation mechanisms) or through post-copulatory sperm competition (via sperm storage).
Investigation of the Effects of Zebularine on Caspase-3 and Caspase-9 Involved in Anticancer and Apoptotic Mechanisms in SKBR3 Breast Cancer Cell Line

The aim of this study is to investigate the anti-proliferative effects of Zebularine on caspase-3 and 9 genes by methylation and protein expression on SKBR3 cells. The SKBR3 cells were treated with Zebularine at different concentrations (0 - 140 μM) for 24 - 96 hours and the effects on the cell viability were shown. The effects on the cell migration and cell transformation were also demonstrated at the specified dose (IC50 = 40 μM). At the same time, the HRM method and western-blot analysis were used to understand the effects in the apoptotic mechanism. According to the obtained results, it was observed that Zebularine significantly decreased the cell proliferation, cell migration and the cell growth (p it was observed that Zebularine caused a decrease in both caspase-3 and 9 methylation rates at the 72nd hour. However, both reductions in the methylation levels didn’t cause a significant change (p > 0.05). At the same time, it was detected by the western-blot analyses that there was a time dependent increase in caspase-3 genes while a decrease was detected in the caspase-9 gene in progress of time. The obtained results showed that the methylation changes occurring in the caspase genes weren’t related to the protein levels. According to these results, supportive results are obtained showing that the Zebularine can be used in chemotherapy and that this study is unique since it is the first study in the literature intending to investigate the effects of Zebularine on the SKBR3 cells.
Assessing the trophic ecology of three sympatric squid in the marine ecosystem off the Patagonian Shelf by combining stomach content and stable isotopic analyses

ABSTRACT Squid species are important components of the Southern Atlantic Ocean ecosystems, as they prey on a wide range of crustaceans, fish and cephalopods. As a result of this trophic interaction and their high abundance, they are considered reliable indicators of energy transfer and biomass in the food web. We identified Illex argentinus, Doryteuthis gahi and Onykia ingens as the most important squid species interacting on the Patagonian shelf, and used isotope analysis and stomach content identification to assess the feeding ecology and interaction of these squids in the ecosystem. Our results describe trophic interactions by direct predation of O. ingens and I. argentinus on D. gahi, and a trophic overlap of the three squid, and indicate a higher trophic level and differences in the foraging areas for mature and maturing D. gahi inferred through δ15N and δ13C concentrations. These differences were related to the segregation and different habitat of large mature D. gahi and suggest a food enrichment of C and N based on feeding sources other than those used by small maturing D. gahi and I. argentinus and O. ingens.
International Ultraviolet Explorer atlas of B-type spectra from 1200 to 1900 Å.

The IUE archives provide an unprecedented sample of uniform, high-quality ultraviolet stellar spectra. In particular, they contain high-resolution SWP data for nearly 200 different O stars. We have undertaken a survey of the 1200-1900 A region in about 120 of them having homogeneous optical spectral classifications to investigate systematically the behavior of the ultraviolet features, incuding the prominent stellar wind profiles and the degree to which they correlate with the optical types. The standard extracted spectrograms have been rebinned to a constant wavelength resolution of 0.25A and uniformly normalized (not dereddened) at the GSFC RDAF. They are then plotted at 10A/cm, with reseau, photometric quality and echelle order junction flags available. This atlas contains such plots for about 100 stars, arranged in spectral-type, luminosity and peculiar object sequences. The results show a high degree of correlation between the ultraviolet features, both photospheric and stellar-wind, and the optical classifications for the majority of the O-type stars.
An experiment in mnemonic imagery in adult basic education science instruction

AN EXPERIMENT IN MNEMONIC IMAGERY IN ADULT BASIC EDUCATION SCIENCE INSTRUCTION This study was designed to investigate the effects of instructor provided mnemonic imagery on the learning of science content and problem solving. The experiment was conducted with adult basic education students(N=40). A number of visual anagrams were constructed which provided both the content and application of science rules. Mnemonic imagery instruction resulted in significantly higher performance on both formula recognition and multi-step problem solving tests. ACKNOWLEDGMENTS ABSTRACT TABLE OF CONTENTS CHAPTER
Preliminary Research on Arbuscular Mycorrhizal Fungi of Dipterocarpaceae in Xishuangbanna,Southern Yunnan

In order to investigate the status of arbuscular mycorrhizae (AM) in tropical dipterocarpaceae forest, rhizospheric soil with roots of seventeen species of dipterocarpaceae plants grown in both native forests and plantations in tropical region Xishuangbanna, Yunnan Province, were sampled to determine their mycorrhizal colonization, spore density, isolation frequency, relative abundance and species richness of AM fungi. The mycorrhizal colonization rate of host plants were measured under a BX50 Olympus Microscope with Automatic Photo Micrographic System (320_640×) after staining with 0.5% acid fuchsin. Spores of AM fungi in aliquots (20 ml) of each soil were isolated by wet_sieving decanting and cane sugar centrifugal method, which were used to identify according to the newest taxonomic system described by Morton and Redecker (2001). Species richness was measured as the number of species compared with the number of individuals in the community. Results showed that most roots collected were colonized by AM fungi, typical arbuscules and vesicles were formed with various colonization rates (up to 40%, grade VIII), and 0_1. 86 vesicles per mm root length. Species richness on Parashorea chinensis was the highest (4.31), while Shorea assamica showed the lowest (1.63). Hopea mollissima gave the maximum spore density (7.76), Vatica astrotricha the minimal (3.09). Thirty_two AM fungal species belonging to the genera Acaulospora, Glomus, Paraglomus, Achaeospora and Scutellospora were identified. The frequencies and relative abundances of AM fungi varied. Acaulospora and Glomus were likely the dominant proportion in the rhizospheric soil of dipterocarpus plants in the tropical region. It was suggested that most dipterocarpus plants can form typical AM and there was high species diversity of AM fungi in tropical dipterocarpaceae forest. The functional role of these AM fungi in the physiology of dipterocarpus plants needs further research.
Pestivirus seroprevalence in sheep populations from inland and coastal zones of Turkey

Au cours de cette etude, la seroprevalence des pestivirus chez le mouton a ete etablie dans 11 provinces turques situees en zone cotiere (6 provinces) ou en zone interieure (5 provinces) a partir de 2444 animaux par un test de sero-neutralisation utilisant la souche NADL du BVDV (Bovine Viral Diarrhoea Virus). La seroprevalence globale a ete de 18.94 % (463/2444) et la valeur moyenne de la seroprevalence du BVDV observee dans la region interieure (caracterisee par un climat froid) a ete significativement plus elevee que celle observee dans la region cotiere (avec un climat chaud) (p < 0.05). La distribution des titres en anticorps a differe significativement entre les populations ovines de ces 2 regions (p < 0.05): les proportions des titres intermediaires (1:20 - 1:40) ont ete nettement augmentees au sein de la population cotiere alors que les faibles titres (1:5 - 1:10) ont ete plus souvent observees chez les ovins de la region interieure. Par consequent, les infections par les pestivirus pourraient etre enzootiques dans cette zone et le climat, particulierement un climat froid, serait un element preponderant dans le developpement du virus.