Miracle-dz/UniFAR-FTUs huggingface dataset:
Effectiveness of Fluorescence-Based Methods to Detect in situ Demineralization and Remineralization on Smooth Surfaces

This study aimed to evaluate the effectiveness of fluorescence-based methods (DIAGNOdent, LF; DIAGNOdent pen, LFpen, and VistaProof fluorescence camera, FC) in detecting demineralization and remineralization on smooth surfaces in situ. Ten volunteers wore acrylic palatal appliances, each containing 6 enamel blocks that were demineralized for 14 days by exposure to a 20% sucrose solution and 3 of them were remineralized for 7 days with fluoride dentifrice. Sixty enamel blocks were evaluated at baseline, after demineralization and 30 blocks after remineralization by two examiners using LF, LFpen and FC. They were submitted to surface microhardness (SMH) and cross-sectional microhardness analysis. The integrated loss of surface hardness (ΔKHN) was calculated. The intraclass correlation coefficient for interexaminer reproducibility ranged from 0.21 (FC) to 0.86 (LFpen). SMH, LF and LFpen values presented significant differences among the three phases. However, FC fluorescence values showed no significant differences between the demineralization and remineralization phases. Fluorescence values for baseline, demineralized and remineralized enamel were, respectively, 5.4 ± 1.0, 9.2 ± 2.2 and 7.0 ± 1.5 for LF; 10.5 ± 2.0, 15.0 ± 3.2 and 12.5 ± 2.9 for LFpen, and 1.0 ± 0.0, 1.0 ± 0.1 and 1.0 ± 0.1 for FC. SMH and ΔKHN showed significant differences between demineralization and remineralization phases. There was a negative and significant correlation between SMH and LF and LFpen in the remineralization phase. In conclusion, LF and LFpen devices were effective in detecting demineralization and remineralization on smooth surfaces provoked in situ.
Concurrent alcoholism and social anxiety disorder: a first step toward developing effective treatments.

BACKGROUND Social anxiety disorder (also called social phobia) is an anxiety disorder in which affected individuals fear the scrutiny of others. Clinical reports suggest that individuals with social anxiety disorder often use alcohol to alleviate anxiety symptoms, a practice that leads to alcohol abuse and/or dependence in approximately 20% of affected individuals. The present study investigated whether simultaneous treatment of social phobia and alcoholism, compared with treatment of alcoholism alone, improved alcohol use and social anxiety for clients with dual diagnoses of social anxiety disorder and alcohol dependence. METHODS The design was a two-group, randomized clinical trial that used 12 weeks of individual cognitive behavioral therapy for alcoholism only (n = 44) or concurrent treatment for both alcohol and social anxiety problems (n = 49). Outcome data were collected at the end of 12 weeks of treatment and at 3 months after the end of treatment. RESULTS Results with intent-to-treat analyses showed that both groups improved on alcohol-related outcomes and social anxiety after treatment. With baseline scores covaried, there was a significant effect of treatment group on several drinking measures. Counter to the hypothesis, the group treated for both alcohol and social anxiety problems had worse outcomes on three of the four alcohol use indices. No treatment group effects were observed on social anxiety indices. CONCLUSIONS Implications for the staging of treatments for coexisting social phobia and alcoholism are discussed, as well as ways that modality of treatments might impact outcomes.
Craniometric Patterning within Ancient Peru

Numerous studies have used the Howells Peruvian sample from the province of Yauyos as the morphological representative for South America. Although Peru has a rich history of investigations of human skeletal remains, biological distance studies based on craniometrics are limited to nonexistent. This study examines the morphological variation found in Peru using the Howells sample and three additional coastal (Makatampu and Ancón) and highland (Cajamarca) samples. The between-group variation and the degree of among-group differentiation were tested using a canonical discriminant analysis and Mahalanobis D², respectively. All groups are significantly different from the Yauyos sample and results indicate a closer morphological affinity between coastal groups and the two highland groups.
Identification of Patient Venous Thromboembolism Risk Across the Continuum of Care

Venous thromboembolism (VTE) complications are the leading cause of preventable in-hospital mortality and morbidity in the United States. Initiatives by the National Quality Forum, the Joint Commission, and the Surgical Care Improvement Project aim to improve the prevention of VTE and emphasize the need to recognize the risk of the condition in hospitalized patients. In clinical practice, individual risk assessment using a validated scoring system provides patients with the best care in the prevention of VTE. This is accomplished by a weighted scoring of risk factors, selection of the most appropriate prevention strategy for patients at risk, and regular risk review across the continuum of care. All hospitals should have a local, written, care pathway which assesses inpatient risk of VTE as early as possible upon admission and identifies members of the health care team responsible for applying risk assessment. Venous thromboembolism risk should be regularly reassessed for any changes in the level of risk, with extended out-of-hospital prophylaxis considered for patients with continued risk factors, such as prolonged immobility or illness, treated at home, or in a long-term care facility. Finally, a mandatory alert system requiring the clinician to address the issue of prophylaxis before any orders are carried out by the nursing staff is one way to protect all hospitalized patients.
Sequence Variation within Botulinum Neurotoxin Serotypes Impacts Antibody Binding and Neutralization

ABSTRACT The botulinum neurotoxins (BoNTs) are category A biothreat agents which have been the focus of intensive efforts to develop vaccines and antibody-based prophylaxis and treatment. Such approaches must take into account the extensive BoNT sequence variability; the seven BoNT serotypes differ by up to 70% at the amino acid level. Here, we have analyzed 49 complete published sequences of BoNTs and show that all toxins also exhibit variability within serotypes ranging between 2.6 and 31.6%. To determine the impact of such sequence differences on immune recognition, we studied the binding and neutralization capacity of six BoNT serotype A (BoNT/A) monoclonal antibodies (MAbs) to BoNT/A1 and BoNT/A2, which differ by 10% at the amino acid level. While all six MAbs bound BoNT/A1 with high affinity, three of the six MAbs showed a marked reduction in binding affinity of 500- to more than 1,000-fold to BoNT/A2 toxin. Binding results predicted in vivo toxin neutralization; MAbs or MAb combinations that potently neutralized A1 toxin but did not bind A2 toxin had minimal neutralizing capacity for A2 toxin. This was most striking for a combination of three binding domain MAbs which together neutralized >40,000 mouse 50% lethal doses (LD50s) of A1 toxin but less than 500 LD50s of A2 toxin. Combining three MAbs which bound both A1 and A2 toxins potently neutralized both toxins. We conclude that sequence variability exists within all toxin serotypes, and this impacts monoclonal antibody binding and neutralization. Such subtype sequence variability must be accounted for when generating and evaluating diagnostic and therapeutic antibodies.
Lyophilization monophase solution technique for preparation of amorphous flutamide dispersions

Flutamide (FLT) is a poorly soluble anticancer drug. Therefore, lyophilized dispersions (LDs) of FLT with polyvinylpyrrolidone (PVP) K30, polyethylene glycol (PEG) 6000, and pluronic F127 were prepared via lyophilization monophase solution technique with the aim of increasing its dissolution rate. FLT showed an AL-type phase solubility diagrams with PVP and PEG, whereas AN-type diagram was obtained with pluronic. The amount of residual tertiary butyl alcohol, determined by gas chromatography, was 0.015–0.021% w/w. Differential scanning calorimetry and X-ray diffractometry revealed that FLT–polymer 1:1 LDs were partially amorphous, whereas the 1:3 and 1:5 LDs were completely amorphous. After 6 months storage, polymers under study inhibited FLT recrystallization maintaining its amorphous form. The particle size of FLT–polymer LDs was between 0.81 and 2.13 μm, with a high surface area (268.43–510.82 m2/g) and porosity (354.01–676.23 e−3 mL/g). Also, the poor flow properties of FLT could be improved but to a limited extent. FLT dissolution was significantly enhanced with the fastest dissolution that was achieved using pluronic. After 30 min, about 66.52%, 78.23%, and 81.64% of FLT was dissolved from 1:5 FLT–PVP, PEG, and pluronic LDs, respectively, compared with only 13.45% of FLT. These data suggest that these polymers might be useful adjuncts in preparation and stabilization of amorphous immediate-release FLT LDs.
Motility and infectivity of Plasmodium berghei sporozoites expressing avian Plasmodium gallinaceum circumsporozoite protein

Avian and rodent malaria sporozoites selectively invade different vertebrate cell types, namely macrophages and hepatocytes, and develop in distantly related vector species. To investigate the role of the circumsporozoite (CS) protein in determining parasite survival in different vector species and vertebrate host cell types, we replaced the endogenous CS protein gene of the rodent malaria parasite Plasmodium berghei with that of the avian parasite P. gallinaceum and control rodent parasite P. yoelii. In anopheline mosquitoes, P. berghei parasites carrying P. gallinaceum and rodent parasite P. yoelii CS protein gene developed into oocysts and sporozoites. Plasmodium gallinaceum CS expressing transgenic sporozoites, although motile, failed to invade mosquito salivary glands and to infect mice, which suggests that motility alone is not sufficient for invasion. Notably, a percentage of infected Anopheles stephensi mosquitoes showed melanotic encapsulation of late stage oocysts. This was not observed in control infections or in A. gambiae infections. These findings shed new light on the role of the CS protein in the interaction of the parasite with both the mosquito vector and the rodent host.
Synthesis, Degradation, and Antimicrobial Properties of Targeted Macromolecular Prodrugs of Norfloxacin

ABSTRACT Long-term antibiotic treatment is required to cure tuberculosis. Targeted antibiotics should improve the efficacy of treatment by concentrating the drugs close to the bacteria. The aim of the present study was to synthesize targeted conjugates. For this purpose, we used mannose as a homing device to direct norfloxacin into macrophages. Dextran was used as the polymer bearing both mannose and norfloxacin. Using different peptide spacer arms to link norfloxacin to dextran, we demonstrated that norfloxacin acts as an antibiotic only when it is released in its native form. Also, targeting by using mannose as a homing device is required to achieve antimycobacterial activity in vivo. Thus, norfloxacin, which is inactive against mycobacteria in its native form in vivo, can be transformed into an active drug by targeting.
Altered Mitochondrial Sensitivity for ADP and Maintenance of Creatine-stimulated Respiration in Oxidative Striated Muscles from VDAC1-deficient Mice*

Voltage-dependent anion channels (VDACs) form the main pathway for metabolites across the mitochondrial outer membrane. The mouse vdac1 gene has been disrupted by gene targeting, and the resulting mutant mice have been examined for defects in muscle physiology. To test the hypothesis that VDAC1 constitutes a pathway for ADP translocation into mitochondria, the apparent mitochondrial sensitivity for ADP (K m (ADP)) and the calculated rate of respiration in the presence of the maximal ADP concentration (V max) have been assessed using skinned fibers prepared from two oxidative muscles (ventricle and soleus) and a glycolytic muscle (gastrocnemius) in control andvdac1 − /− mice. We observed a significant increase in the apparentK m (ADP) in heart and gastrocnemius, whereas the V max remained unchanged in both muscles. In contrast, a significant decrease in both the apparentK m (ADP) andV max was observed in soleus. To test whether VDAC1 is required for creatine stimulation of mitochondrial respiration in oxidative muscles, the apparentK m (ADP) andV max were determined in the presence of 25 mm creatine. The creatine effect on mitochondrial respiration was unchanged in both heart and soleus. These data, together with the significant increase in citrate synthase activity in heart, but not in soleus and gastrocnemius, suggest that distinct metabolic responses to altered mitochondrial outer membrane permeability occur in these different striated muscle types.
Johann Sebastian Bach: Life and Work

This major study of the composer's life and work follows the course of Bach's career in rich detail - from his humble beginnings as an organ tuner and self-taught musician, to his role as Kapellmeister and cantor of St. Thomas' Church in Leipzig. It explores Bach's relations with the German aristocracy, the Church and contemporary theological debates, his perfectionism, and his role as the devoted head of a large family. The author also carefully analyses Bach's innovations in harmony and counterpoint, placing them in the context of European musical and social history.
Controversies in the Assessment of HER-2: More Questions Than Answers

Human epidermal growth factor receptor 2 (HER-2) is over-expressed in 15% to 30% of breast cancers and is a poor prognostic marker in node-positive patients. HER-2 expression is an indicator of greater sensitivity to anthracycline-based chemotherapy and is the major criterion for selection for treatment with the anti–HER-2 antibody trastuzumab (Herceptin). Fluorescence in situ hybridization and immunohistochemistry (IHC) are the 2 most commonly used methods for detection of the gene and protein, respectively. Criticisms have been levied at the IHC method of identifying HER-2 overexpression but convenience and costs of this technique cannot be overlooked. Modifications to the IHC technique and scoring accommodate for many of the problems that derive from variables in preanalytical and analytic factors that influence results but standardization is currently impossible to attain. Deficiencies in fluorescence in situ hybridization assay also exist and alternative molecular methods of assay are explored in this review.
Psychiatric Severity and Spirituality, Helping, and Participation in Alcoholics Anonymous During Recovery

Although helping others is a critical part of Alcoholics Anonymous (AA) and many treatment programs, measures for assessing helping and describing its relationship with sobriety are lacking. A sample of 200 subjects completed a Helper Therapy Scale including three subscales: Recovery Helping (alpha = 0.78), Life Helping (alpha = 0.62), and Community Helping (alpha = 0.60). A previous analysis using structural equation modeling found that length of sobriety predicted measures of spirituality, helping, and AA participation. The analysis reported here examined whether psychiatric severity was associated with these variables. Results indicated significant relationships between psychiatric severity and measures of spirituality (Self Transcendence, Forgiveness, Positive Coping, and Negative Coping) and AA Achievement (defined as completing the 12 steps and serving as a sponsor). However, no relationships were found between psychiatric severity and length of sobriety, the three Helper Therapy subscales, or AA involvement. The findings suggest that individuals with higher psychiatric severity may need assistance from their peers or professional service providers to develop a spiritual life, serve as a sponsor for others, or complete the steps of AA.
Effects of Operating Frequency on Luminance Characteristics of Wide-Gap AC Plasma Display Panel

The three-electrode microdischarge characteristics of ac plasma display panels are analyzed with a wide-sustain-discharge gap of 180 mum. In particular, the luminance and consumed-power variations are examined as parameters of the operating frequency. It is found that the luminance per unit pulse decreases, and simultaneously, the consumed power per unit pulse increases with an increase in the operating frequency. For the wide-gap structure, the sustain voltage increases when increasing the operating frequency, thus causing the power consumption per unit pulse to be increased. However, the luminance per unit pulse decreases when increasing the sustain voltage, even though the power consumption per unit pulse increases with the sustain-voltage increase. As a result, it is observed that the self-erasing discharge between the sustain and address electrodes caused by the short pulsewidth under high frequency affects the luminance and consumed power. Due to the self-erasing discharge, the sustain voltage increases, and the discharge shrinkage phenomenon affecting the luminance characteristics occurs under high frequency that is up to 200 kHz with a wide-sustain-discharge gap of 180 mum.
Distinct Patterns of Neuronal Loss and Alzheimer's Disease Lesion Distribution in Elderly Individuals Older than 90 Years

To explore the characteristics of brain aging in very old individuals, we performed a quantitative analysis of neurofibrillary tangle (NFT) and senile plaque (SP) distribution and neuron densities in 13 nondemented patients, 15 patients with very mild cognitive impairment, and 22 patients with Alzheimer's disease (AD), all older than 96 years of age. Non-demented cases displayed substantial NFT formation in the CA1 field and entorhinal cortex only. Very mild cognitive impairment cases were characterized by the presence of high NFT densities in layers V and VI of area 20, and AD cases had very high NFT densities in the CA1 field compared to nondemented cases. Moreover, high SP densities were found in areas 9 and 20 in AD, but not in cases with very mild cognitive impairment and nondemented cases. In contrast to previous reports concerning younger demented patients, neuron densities were preserved in the CA1 field, dentate hilus, and subiculum in centenarians with AD. In these cases, there was a marked neuronal loss in layers II and V of the entorhinal cortex, and in areas 9 and 20. In the present series, no correlation was found between neurofibrillary tangle and neuron densities in the areas studied, whereas there was a negative correlation between senile plaque and neuron densities in area 20. The comparison between the present data and those reported previously concerning younger cohorts suggests that there is a differential cortical vulnerability to the degenerative process near the upper age-limit of.
HLA-E surface expression depends on binding of TAP-dependent peptides derived from certain HLA class I signal sequences.

Previous studies showed that HLA-E was expressed in lymphoblastoid cell line (LCL) 721.221 cells, but surface expression was lacking. To determine the signals controlling surface expression, we constructed a series of hybrid genes using complementary portions derived from the HLA-E and HLA-A2 genes. In this manner, a hybrid of HLA-E was identified, designated AEH, which differed from HLA-E by having the HLA-A2 signal sequence substituting for the HLA-E leader peptide. Transfection of LCL 721.221 cells with AEH induced HLA-E surface expression. Analysis of peptides bound to HLA-E revealed that a nonamer peptide derived from the A2 signal sequence was the predominant peptide bound. LCL 721.221 cells transfected with certain class I genes, including HLA-G, were also sufficient to promote peptide binding and HLA-E surface expression without increasing the level of HLA-E heavy chain synthesis. Peptides bound to HLA-E consisted of nine amino acids, with methionine at position 2 and leucine in the carboxyl-terminal position, and were nearly identical to the leader sequence-derived peptide previously shown to be a predominant peptide bound to the murine Qa-1 Ag. Signal peptides derived from certain HLA-B proteins with threonine in position 2 only marginally up-regulated HLA-E surface expression in . 221 cells. An examination of HLA-E peptide binding in the TAP negative cell line . 134 indicated that peptide binding to HLA-E was dependent on a functional TAP heterodimer regardless of whether peptide was available in cis, as in the AEH construct, or in trans, as in the class I transfectants of . 221 cells.
Gastric protein breakdown and pancreatic enzyme activities in response to two different dietary protein sources in newly weaned pigs.

Seventy pigs were weaned at 25 d of age and fed diets based on either skim-milk powder (SMP) or soybean protein concentrate (SOY). At 0, 3, 6, and 10 d after weaning, pigs were anesthetized, their pancreases were removed, and digesta were collected from different sections of the digestive tract. The ratio between trichloroacetic acid (TCA)-precipitable protein and total (crude) protein (pp:cp) in gastric digesta was higher with SOY feed than with SMP feed. In the jejunum, no difference was found; hence, the degree of protein breakdown in jejunal chyme did not differ between protein sources. Trypsin activities in jejunal chyme and in pancreatic tissue increased (P < .01) after weaning. Chymotrypsin activity in pancreatic tissue tended to decrease after weaning and did not reach "weaning levels" for at least 10 d. Pancreatic trypsin developed more rapidly than chymotrypsin after weaning. Chymotrypsin activities in jejunal digesta were higher (P < .05) for the pigs fed SMP than for those fed SOY. Protease activities in the jejunum at d 6 after weaning were clearly affected (P < .05) by feed intake after weaning. The ratio between trypsin and chymotrypsin activity in jejunal chyme was higher (P < .05) for SOY-fed pigs than for SMP-fed pigs. It was concluded that the stomach plays an important role in the digestion of milk protein and that the development of pancreatic proteases after weaning (synthesis, secretion, breakdown) depends on feed intake and on dietary protein source.
Remembering the Holocaust in Germany, 1945-2000 German Strategies and Jewish Responses

Ten authors from five countries present a variety of fresh analyses of the strategies Germans have adopted in coping with the Nazi past. Through historical, sociological, educational, and cultural approaches the unresolved tensions existing in German society – between the will to be accepted as an integral part of western civilization and to put the Nazi chapter in general and the Holocaust in particular behind, on the one hand, and an awareness of responsibility combined with recurring, sometimes sudden, manifestations of long-term results and implications of the past, on the other – are analyzed. Through its multifaceted approach, this book contributes to a better understanding of present-day German society and of Germany’s delicate relationships with both the United States and Israel. Contents: Dan Michman: Introduction – Jeffrey Herf: The Holocaust and the Competition of Memories in Germany, 1945-1999 – Gilad Margalit: Divided Memory? Expressions of a United German Memory – Y. Michal Bodemann: The Uncanny Clatter: The Holocaust in Germany before Its Mass Commemoration – Inge Marszolek: Memory and Amnesia: A Comment on the Lectures by Gilad Margalit and Michal Bodemann – Chris Lorenz: Border-crossings: Some Reflections on the Role of German Historians in Recent Public Debates on Nazi History – Dan Diner: The Irreconcilability of an Event: Integrating the Holocaust into the Narrative of the Century – Michael Brenner: The Changing Role of the Holocaust in the German-Jewish Public Voice – Shlomo Shafir: Constantly Disturbing the German Conscience: The Impact of American Jewry – Yehuda Ben-Avner: Ambivalent Cooperation: The German-Israeli Joint Committee on Schoolbooks – Yfaat Weiss: The Vague Echoes of German Discourse in Israel.