Miracle-dz/UniFAR-FTUs huggingface dataset:
Age-Related Decline in Vertical Jumping Performance in Masters Track and Field Athletes: Concomitant Influence of Body Composition

Vertical jumping power declines with advancing age, which is theoretically explicable by loss of muscle mass and increases in body fat. However, the results of previous cross-sectional studies remain inconsistent on these relationships. The present study included 256 masters athletes who competed at the 2018 track and field world championships in Málaga, Spain. We assessed body composition with bioelectrical impedance (Inbody S10) and vertical jumping power with a Leonardo ground reaction force platform. Relationships between age, jumping power, and body composition were analyzed by correlation and regression analyses. Hierarchical multiple regression analysis was used to evaluate effects of each factor on vertical jumping power. Age-related rates of decreases in maximal power and jump height were similar between male and female athletes. Percent fat-free mass and percent body fat were negatively and positively, respectively, associated with age in masters athletes and were comparable to those previously observed in the general population. Moreover, these effects in body composition can, to a great extent, explain the age-related decline in jumping power, an effect that seems at least partly independent of age. Finally, the multiple regression model to determine independent predictors of vertical jump performance yielded an overall R2 value of 0.75 with the inclusion of (1) athletic specialization in power events, (2) percent fat-free mass, and (3) phase angle. However, partial regression yielded significant effects of age, but not gender, on peak power, even when adjusting for athletic specialization, percent fat-free mass, and phase angle. We concluded that loss of skeletal muscle mass and changes in bio-impedance phase angle are important contributors to the age-related reduction in anaerobic power, even in adults who maintain high levels of physical activity into old age. However, age per se remains a significant predictor of vertical jump performance, further demonstrating deteriorated muscle quality at old age (sarcosthenia).
Photosynthetic hydrogen production: Novel protocols, promising engineering approaches and application of semi-synthetic hydrogenases.

﻿Photosynthetic production of molecular hydrogen (H2 ) by cyanobacteria and green algae is a potential source of renewable energy. These organisms are capable of water biophotolysis by taking advantage of photosynthetic apparatus that links water oxidation at photosystem II and reduction of protons to H2 downstream of photosystem I. Although the process has a theoretical potential to displace fossil fuels, photosynthetic ﻿H2 production in its current state is not yet efficient enough for industrial applications due to a number of physiological, biochemical, and engineering barriers. This article presents a short overview of the metabolic pathways and enzymes involved in H2 photoproduction in cyanobacteria and green algae and our present understanding of the mechanisms of this process. We also summarize recent advances in engineering photosynthetic cell factories capable of overcoming the major barriers to efficient and sustainable H2 production.
MYCOBACTERIA IN SKIN LESIONS AND THE HABITAT OF THE ENDANGERED HOUSTON TOAD (ANAXYRUS HOUSTONENSIS)

Abstract: Head-starting of the federally endangered Houston toad (Anaxyrus houstonensis), that is, the release of egg strands, tadpoles, and metamorphic juveniles produced in captivity into the original breeding ponds, requires assessment of potential threats for the transmission of pathogens from captive to free-ranging toads. We used Illumina-based 16S rRNA V3 amplicon sequencing to investigate the community structure of bacteria from skin lesions of captive Houston toad and habitat (pond) samples. Proteobacteria, alone or together with Actinobacteria and, in some samples, Cyanobacteria represented virtually all reads in tissue lesion samples, whereas pond samples were much more diverse, with Acidobacteria, Actinobacteria, Bacteriodetes, Chloroflexi, Cyanobacteria, Firmicutes, Planctomycetes, Proteobacteria, and Verrucomicrobia present with little variation between samples. If present in lesions, Actinobacteria were largely represented by Mycobacteriaceae, and here mainly by one sequence identical to sequences of members of the Mycobacterium chelonae–abscessus complex. In pond samples, mycobacteria represented only a small portion of the actinobacteria, although at higher diversity with six distinct reads. Sequences for reads obtained from pond samples were identical to those representing the M. chelonae–abscessus complex, a group with Mycobacterium marinum, Mycobacterium kansasii, Mycobacterium avium, a group with Mycobacterium vaccae, Mycobacterium fortuitum, Mycobacterium poriferae, and a group with Mycobacterium elephantis and Mycobacterium celeriflavum, whereas sequences of high similarity were detected for reads related to those of Mycobacterium holsaticum, Mycobacterium pallens, and Mycobacterium obuense, and Mycobacterium goodii. Our results indicated that lesions observed on the Houston toad in captivity are not the result of mycobacteria in every case, and that the presence of mycobacteria in the captive colony does not represent a novel pathogen threat to the wild populations because such bacteria are also seen in the natural pond habitats for the Houston toad.
Single START-domain protein Mtsp17 is involved in transcriptional regulation in Mycobacterium smegmatis

Tuberculosis caused by the pathogen Mycobacterium tuberculosis (MTB), remains a significant threat to global health. Elucidating the mechanisms of essential MTB genes provides an important theoretical basis for drug exploitation. Gene mtsp17 is essential and is conserved in the Mycobacterium genus. Although Mtsp17 has a structure closely resembling typical steroidogenic acute regulatory protein-related lipid transfer (START) family proteins, its biological function is different. This study characterizes the transcriptomes of Mycobacterium smegmatis to explore the consequences of mtsp17 downregulation on gene expression. Suppression of the mtsp17 gene resulted in significant down-regulation of 3% and upregulation of 1% of all protein-coding genes. Expression of desA1, an essential gene involved in mycolic acid synthesis, and the anti-SigF antagonist MSMEG_0586 were down-regulated in the conditional Mtsp17 knockout mutant and up-regulated in the Mtsp17 over-expression strain. Trends in the changes of 70 of the 79 differentially expressed genes (Log2 fold change > 1.5) in the conditional Mtsp17 knockout strain were the same as in the SigF knockout strain. Our data suggest that Mtsp17 is likely an activator of desA1 and Mtsp17 regulates the SigF regulon by SigF regulatory pathways through the anti-SigF antagonist MSMEG_0586. Our findings indicate the role of Mtsp17 may be in transcriptional regulation, provide new insights into the molecular mechanisms of START family proteins, and uncover a new node in the regulatory network of mycobacteria.
GoAmazon 2014/15 Thermal Desorption Chemical Ionization Mass Spectrometer (TDCIMS) Field Campaign Report

The Thermal Desorption Chemical Ionization Mass Spectrometer (TDCIMS) deployment to the U.S. Department of Energy (DOE)’s Atmospheric Radiation Measurement (ARM) Climate Research Facility T3 site in Manacapuru, Brazil, was motivated by two main scientific objectives of the Green Ocean Amazon (GoAmazon) 2014/15 field campaign. 1) Study the interactions between anthropogenic and biogenic emissions by determining important molecular species in ambient nanoparticles. To address this, TDCIMS data will be combined with coincident measurements such as gas-phase sulfuric acid to determine the contribution of sulfuric acid condensation to nucleation and growth. We can then compare that result to TDCIMS-derived nanoparticle composition to determine the fraction of growth that can be attributed to the uptake of organic compounds. The molecular composition of sampled particles will also be used to attribute specific chemical species and mechanisms to growth, such as the condensation of low-volatility species or the oligomerization of α-dicarbonyl compounds. 2) Determine the source of new ambient nanoparticles in the Amazon. The hypothesis prior to measurements was that potassium salts formed from the evaporation of primary particles emitted by fungal spores can provide a unique and important pathway for new particle production in the Amazon basin. To explore this hypothesis, the TDCIMS recordedmore » the mass spectra of sampled ambient particles using a protonated water cluster Chemical Ionization Mass Spectrometer (CIMS). Laboratory tests performed using potassium salts show that the TDCIMS can detect potassium with high sensitivity with this technique. « less
Metagenomic insights into mixotrophic denitrification facilitated nitrogen removal in a full-scale A2/O wastewater treatment plant

Wastewater treatment plants (WWTPs) are important for pollutant removal from wastewater, elimination of point discharges of nutrients into the environment and water resource protection. The anaerobic/anoxic/oxic (A2/O) process is widely used in WWTPs for nitrogen removal, but the requirement for additional organics to ensure a suitable nitrogen removal efficiency makes this process costly and energy consuming. In this study, we report mixotrophic denitrification at a low COD (chemical oxygen demand)/TN (total nitrogen) ratio in a full-scale A2/O WWTP with relatively high sulfate in the inlet. Nitrogen and sulfur species analysis in different units of this A2/O WWTP showed that the internal sulfur cycle of sulfate reduction and reoxidation occurred and that the reduced sulfur species might contribute to denitrification. Microbial community analysis revealed that Thiobacillus, an autotrophic sulfur-oxidizing denitrifier, dominated the activated sludge bacterial community. Metagenomics data also supported the potential of sulfur-based denitrification when high levels of denitrification occurred, and sulfur oxidation and sulfate reduction genes coexisted in the activated sludge. Although most of the denitrification genes were affiliated with heterotrophic denitrifiers with high abundance, the narG and napA genes were mainly associated with autotrophic sulfur-oxidizing denitrifiers. The functional genes related to nitrogen removal were actively expressed even in the unit containing relatively highly reduced sulfur species, indicating that the mixotrophic denitrification process in A2/O could overcome not only a shortage of carbon sources but also the inhibition by reduced sulfur of nitrification and denitrification. Our results indicate that a mixotrophic denitrification process could be developed in full-scale WWTPs and reduce the requirement for additional carbon sources, which could endow WWTPs with more flexible and adaptable nitrogen removal.
Stability and adsorption of multiphase foam system with high temperature resistance

ABSTRACT The multiphase foam system with high temperature resistance mainly consisted of the foaming agent (0.3% disodium monoester succinate (DMS)) and the foam stabilizer (0.2% PS4 and 0.5% hydrophilic SiO2). The synergy between polysaccharide (PS4) and SiO2 was determined by the foam composite index (Fc), which remained at a higher level after aging for 30 days at 115 °C. Macro and micro structure of foam in the presence or absence of SiO2 was observed with the naked eye and a scanning electron microscope (SEM), respectively; the result confirmed that SiO2 could adsorb on a liquid film. In addition, the effects of crude oil components and pressure on foam properties were investigated; the latter was monitored by a visualized reactor, and the results showed that asphaltene and high pressure boosted foam while it was opposite to aromatic hydrocarbon. Moreover, the static and dynamic adsorption of DMS under different conditions were determined, and the results could be concluded that besides external factors, PS4 could reduce the adsorption of DMS effectively. GRAPHICAL ABSTRACT
Socrates Online: Creating a Sense of Community

Abstract If Socrates taught online, he would enhance learning using questioning, guided inquiry, and problem- and discovery-based learning. He would focus energy on building a sense of community, engaging students with analogies, metaphors, storytelling, and sharing of personal and professional experiences. This article provides nurse educators with examples of the application of the Socratic method to enhance learning and build a sense of belonging to a learning community in online education.
Association between early viral LRTI and subsequent wheezing development, a meta-analysis and sensitivity analyses for studies comparable for confounding factors

Introduction Consideration of confounding factors about the association between Lower Respiratory Tract Infections (LRTI) in childhood and the development of subsequent wheezing has been incompletely described. We determined the association between viral LRTI at ≤ 5 years of age and the development of wheezing in adolescence or adulthood by a meta-analysis and a sensitivity analysis including comparable studies for major confounding factors. Methods We performed searches through Pubmed and Global Index Medicus databases. We selected cohort studies comparing the frequency of subsequent wheezing in children with and without LRTI in childhood regardless of the associated virus. We extracted the publication data, clinical and socio-demographic characteristics of the children, and confounding factors. We analyzed data using random effect model. Results The meta-analysis included 18 publications (22 studies) that met the inclusion criteria. These studies showed that viral LRTI in children ≤ 3 years was associated with an increased risk of subsequent development of wheezing (OR = 3.1, 95% CI = 2.4–3.9). The risk of developing subsequent wheezing was conserved when considering studies with comparable groups for socio-demographic and clinical confounders. Conclusions When considering studies with comparable groups for most confounding factors, our results provided strong evidence for the association between neonatal viral LRTI and the subsequent wheezing development. Further studies, particularly from lower-middle income countries, are needed to investigate the role of non-bronchiolitis and non-HRSV LRTI in the association between viral LRTI in childhood and the wheezing development later. In addition, more studies are needed to investigate the causal effect between childhood viral LRTI and the wheezing development later. Trial registration Review registration: PROSPERO, CRD42018116955; https://www.crd. york.ac. uk/prospero/display_record. php? ID=CRD42018116955.
Stress-responses to ostracism: Examining cortisol and affective reactivity to in-person and online exclusion

Ostracism is associated with poor outcomes, but it is unclear if online versus in-person ostracism elicits divergent psychological and physiological responses. Participants (N = 54) were randomly assigned to online or in-person ostracism, and provided pre- and post-ostracism measures of affect, self-esteem, self-feelings, and salivary cortisol. No significant changes in negative affect, self-esteem, or self-feelings emerged, nor were there differences by ostracism condition. Both ostracism conditions decreased positive affect (stronger online) and lowered cortisol. Extending prior work, ostracism appears to reliably decrease positive affect (especially online) but may not be threatening to the self; moreover, ostracism may reliably elicit acute cortisol declines.
Effectiveness of hepatitis B vaccination in chronic HCV patients after successful generic direct acting antiviral therapy: significance of isolated hepatitis B core antibodies

Previous reports show conflicting results regarding hepatitis B virus (HBV) vaccine efficacy in Hepatitis C virus (HCV) infected individuals and in those with isolated hepatitis B core antibodies (HBcAb). We aimed to evaluate the effectiveness of HBV vaccine and identify possible factors that may contribute to hyporesponsivness in HCV‐treated patients, including those with isolated HBcAb.
Small-angle neutron scattering spectrometer Suanni equipped with ultra-thin biconcave focusing lenses

The small-angle neutron scattering (SANS) spectrometer Suanni at the liquid hydrogen cold neutron source of the 20 MW China Mianyang Research Reactor has recently been upgraded. Ultra-thin biconcave MgF2 lenses with a central thickness down to 0.2 mm have been installed between the collimator chamber and the sample stage. The lenses are able to improve the flux without too excessive an increase in the neutron beam size on the detector. A smaller minimum Q (Qmin) can be obtained by decreasing the beam size without changing the total length of the spectrometer. By testing the central beam profiles under different neutron wavelengths (∼0.56–1 nm) with both traditional pinhole SANS (PSANS) and focusing SANS (FSANS) geometries, the gain factor thanks to the neutron lenses is about one order of magnitude. Given the loss of intensity due to the absorption of neutrons by the lenses, the benefits of the focusing can only be realized if it is possible to increase the aperture size. With an identical source aperture, FSANS can minimize the nominal Qmin from 7.20 × 10−3 nm−1 (for PSANS) to 5.55 × 10−3 nm−1 at a neutron wavelength of 1 nm. The practical benefit provided by the lenses is verified with a solution of poly(methyl methacrylate) nanospheres, which yields a scattering intensity one order of magnitude higher and a better resolution with the FSANS geometry than with that of PSANS.
Attenuated β-adrenergic response in calcium/calmodulin-dependent protein kinase IV-knockout mice

In the present study, we examined the importance of Ca2+/calmodulin-dependent protein kinase IV (CaMKIV) in the regulation of cardiac function using genetically modified CaMKIV-null mice. RT-PCR analysis revealed decreased expression of voltage-dependent calcium channels in the cardiac myocytes of CaMKIV-null mice compared with wild-type mice. CaMKIV-null mice showed shortened QT time on electrocardiograms. Pharmacological analysis revealed decreased responsiveness to the β-adrenergic blocker propranolol in CaMKIV-null mice, whereas the plasma norepinephrine level was not affected. CaMKIV-null mice showed decreased baroreflex on electrocardiograms. Heart rate variability analysis showed unstable R-R intervals, a decreased low frequency power/high frequency power (LF/HF) ratio, and increased standard deviation of the normal to normal R-R intervals (SDNN) in CaMKIV-null mice, suggesting decreased responsiveness to β-adrenergic stimulation in CaMKIV-null mice. Atrial contraction analysis and cardiac action potential recording showed a decreased response to the β-adrenoceptor agonist isoproterenol in CaMKIV-null mice. Furthermore, fluorescence imaging in a CRE-hrGFP assay revealed a decreased response to isoproterenol in CaMKIV-null cardiac myocytes. Taken together, our data strongly suggest a significant effect of CaMKIV gene ablation on cardiac β-adrenergic signal transduction.
Naturally occurring and synthetic peptides: Efficient tyrosinase inhibitors

Tyrosinase is a copper‐containing enzyme involved in the biosynthesis of melanin pigment, which is the most important photo protective agent against skin photo carcinogenesis. Excess production of melanin causes hyperpigmentation leading to undesired browning in human skin, fruits, and vegetable as well as plant‐derived foods. Moreover, the role of tyrosinase in the onset and progression of various diseases such as cancers, Alzheimer's, and Parkinson diseases has been well documented in the literature. In this respect, tyrosinase inhibitors have been in the center of attention particularly as the efficient skin whitening agents. Among a wide range of compounds possessing anti‐tyrosinase activity, peptides both natural and synthetic derivatives have attracted attention due to high potency and safety.
An Adaptive Multi-Scale Retinex Algorithm Realizing High Color Quality and High-Speed Processing

With the popularity of digital still cameras (DSCs), improvement of image quality in dark areas of the image is needed. There are two typical techniques for this: gamma correction and histogram equalization. However, suchtechniques are not always sufficient to improve scene detail in dark areas. Recently, examinations of Retinex theory taking into account the human vision model proposed by Land are being given attention. This algorithm, which utilizes spatial information between surrounding pixels, gives good image detail. Single-scale Retinex (SSR) and Multi-scale Retinex (MSR) are typical Retinex algorithms. However, they raise several practical use issues concerning color images reproduced by printers. In order to address such issues, Adaptive Multi-scale Retinex (AMSR) synthesis of images originally processed by MSR to the original image has been proposed. This algorithm consists of two processes, linear computation and synthesis of both the original image and the image processed by MSR. AMSR can be compared to histogram equalization and MSR for DSC images. AMSR shows that visibility in dark areas can be improved compared to other techniques. Moreover, NEW-AMSR has the following two features: suppression of chromatic unbalance in R, G and B channels, and a high speed processing technique to compute a weighted average.
Response: Genome-Wide Association Study Identifies Two Novel Loci with Sex-Specific Effects for Type 2 Diabetes Mellitus and Glycemic Traits in a Korean Population (Diabetes Metab J 2014;38:375-87)

We greatly appreciate your thoughtful comments and suggestions on our article entitled "Genome-Wide Association Study Identifies Two Novel Loci with Sex-Specific Effects for Type 2 Diabetes Mellitus and Glycemic Traits in a Korean Population," which was published in the Diabetes & Metabolism Journal [1]. Chronic alcohol intake is associated with type 2 diabetes mellitus (T2DM) as an independent risk factor, and it has also been considered to be related with increased adipogenesis, impaired glucose tolerance, and insulin resistance [2]. However, the underlying molecular mechanisms behind physiological and pathological entities have not been fully explored. In our study, we conducted the approximate conditional analysis on multiple diverse effects. We confirmed that the associated signals of two variants (rs11065756 and rs2074356) in CCDC63 and C12orf51 were only slightly diminished after adjustment for alcohol consumptionin T2DM, fasting plasma glucose, and homeostatic model assessment-B traits, indicating that there was no evidence for the substantial attenuation in statistical causal inference. These results suggested that the newly identified T2DM loci were not simply secondary to the alcohol effects. Indeed, a previous genome-wide study showed that the two loci were predominantly associated with the amount of alcohol consumed in two population-based cohorts including heavy drinkers (~40%) [3]. Several studies have reported that moderate alcohol intake is associated with decreased risk of T2DM [4,5,6]. Recent studies have demonstrated that alcohol intake is associated with widespread changes in DNA methylation as an epigenetic factor [7,8]. Given the phenotypic and genotypic heterogeneity between T2DM and alcohol intake, further integrated multianalyses will be needed to determine influence of metastable epialleles on T2DM risk [9]. Taken together, our findings suggest that the two novel variants influencing T2DM have impacts on alcohol-independent T2DM risk. The identification of these loci provides additional clues regarding the pleiotropic effects associated with variation in the 12q24 region of the human genome.