Miracle-dz/UniFAR-FTUs huggingface dataset:
Obtaining and Genetic Stability Analysis of Chinese Cabbage(Brassica oleracea var.capitata)-Cabbage(Brassica compestris ssp.pekinensis)Translocation Lines with Fragment of Cabbage Chromosome 7~#

Translocation line is an individual formed by one-way or reciprocal translocation between chromosomes of two different species.It is significantly important for the application of excellent exogenous gene to study the genetic stability of exogenous chromosome in translocation lines.In order to obtain Chinese cabbage(Brassica oleracea var.capitata)-cabbage(Brassica compestris ssp.pekinensis) translocation lines,Chinese cabbage-cabbage alien addition line 7#(AC7) and its parents Chinese cabbage 85-1 and cabbage 11-1were used as experimental population in present study.Using rays 45 Gy60Co-γ radiating pollens of AC7,M1 plants were obtained by backcrossing irradiated pollens with Chinese cabbage 85-1,and then M2 plants were obtained by selfing M1.Using 31 specific In Del(Insertion-Deletion) markers(compared with Chinese cabbage)distributed in linkage group No.1 of cabbage,and combining with cytological observation,ten translocation lines were identified from selfing progenies of M2 plants.Two translocation lines AT7-1 and AT7-2 with different chromosome fragments of cabbage were selected and studied,for which isolated microspore culture,selfing and backcrossing were further performed.Using specific In Del markers,progeny plants from selfing,backcrossing and microspore culture of two translocation lines were identified.We also had carried on investigation of phenotypic traits in selfing progenies of AT7-1 and AT7-2.The results showed that genetic stability of two translocation lines AT7-1 and AT7-2 with different chromosome fragments of cabbage was different.The genetic stability of selfing progenies,backcross progenies and microspore culture plants in the same translocation line was also different.In AT7-1,the ratio of keeping exogenous fragment in selfing progenies,backcross progenies and microspore culture plants was 73%,28% and 50%,respectively.In AT7-2,the ratio of keeping exogenous fragment in selfing progenies,backcross progenies and microspore culture plants was 84%,62% and 25%,respectively.Head formation related traits in selfing progenies of AT7-1 were segregated,but without segregation in selfing progenies of AT7-2.Studying genetic stability of exogenous chromosomes in Chinese cabbage background could be useful in using new germplasm for Chinese cabbage breeding.
A Prospective, Split-Face, Comparative Study of Combined Treatment With Fractional Microneedle Radiofrequency and Nonablative 1927-nm Fractional Thulium Fiber Laser for Wrinkle Treatment.

BACKGROUND Fractional microneedle radiofrequency (FMR) and nonablative 1927-nm fractional thulium fiber laser (TFL) are widely used for skin rejuvenation treatment. OBJECTIVES To investigate the efficacy and safety of combined treatment with both devices for wrinkles. PATIENTS AND METHODS Twenty-five patients with wrinkles were enrolled. One side of the face was treated with FMR alone, while the other side was treated with a combination of FMR and TFL. Each treatment consisted of 3 sessions at four-week intervals and patients were followed up 12 weeks after the last treatment. Overall improvement was assessed by patient global assessment (PGA) and investigator global assessment (IGA). Depression scores for the evaluation of wrinkles were objectively assessed by Antera 3D system. RESULTS Both sides of the face led to clinical improvement in both mean PGA and IGA. Combination treatment demonstrated a greater improvement in both mean PGA and IGA compared with FMR alone. In addition, wrinkle grading scales and depression scores showed greater improvement in the combination group than in FMR alone. CONCLUSION This study demonstrated that FMR and TFL comprise a good combination treatment for the treatment of wrinkles because both treatments have a synergistic effect on wrinkle improvement.
Effects of IL-23 and IL-27 on the protection and safety of respiratory syncytial virus recombinant protein vaccine

This study performed to investigate the regulatory effects of IL-23, IL-27, and CpG2216 as adjuvants on the cellular immune response induced by recombinant protein vaccine of respiratory syncytial virus(RSV). RSV was propagated and viral titer(TCID50) was measured. Balb/c mice were immunized with G1F/M2 protein(RSV vaccine) and the adjuvants with a interval of 10 days for 3 times, and was phlebotomized at day 10 post-immunization. Ten days after the third immunization, mice were challenged with RSV, and sanctified 5 days later. Samples of lung were collected, and RNA was extracted. RSV-N gene was detected by PCR and fluorescence base real time PCR to evaluate viral replication in lung; cytokines INF-γ, IL-5, Gro, eotaxin, T-bet, GATA3 and gob-5 were tested by fluorescence base real-time PCR; while ections of paraffin embedding lung tissues were stained by HE for histological analysis. The results showed that mice in every group were protected against RSV challenge except mice in PBS group, and the protective efficacy of G1F/M2 with adjuvant was better than that of G1F/M2 without adjuvant. The results of real time PCR showed that the mRNA of INF-γ/IL-5 in IL-27+G1F/M2 group, IL-23 +IL-27 +G1F/M2 group, and IL-23 +CpG2216 +G1F/M2 group were significantly higher than those in the other groups; the mRNA levels of GATA3 in IL-27+G1F/M2 group, IL-23+IL-27+G1F/M2 group, and IL-23+CpG2216 + G1F/M2 group were significantly higher than those in the other groups; the mRNA expression of eotaxin in IL-23+ IL-27+G1F/M2 group was markedly lower than those of the other groups; the mRNA levels of Gro-α in IL-27+G1F/ M2 group, IL-23+IL-27+G1F/M2 group and IL-23+ CpG2216 +G1F/M2 group were lower than that of other groups; the mRNA expression of gob-5 in IL-27+G1F/M2 group and IL-23+IL-27 +G1F/M2 group significantly decreased. Histopathology result showed that except normal control group, lung tissues in the other groups showed inflammatory cells infiltration, including most lymphocytes, a few neutrophils, eosinophils, monocytes and plasma cells, while the number of inflammatory cells in IL-23 +IL-27 +G1F/M2 group was the fewest. In conclusion, the adjuvant IL-23+IL-27 is an ideal choice for RSV recombinant vaccines.
Effect of Zishen Jiangtang Pills on Adipogenic Differentiation of Mice MSC and Its Mechanisms

OBJECTIVE:To investigate the effect of Zishen jiangtang pills on adipogenic differentiation of mice mesenchymal stem cells (MSC) and its mechanisms.METHODS:Mice were given Zishen jiangtang pill via i.g. gtt at a dose of 1.5 g·kg-1 once a day for a continuous week.Serum sample was collected 2 h after last administration.Then,effects of serum containing active components on adipogenic differentiation of MSC were investigated by means of oil red O staining.The expressions of PPARγ2 and FABP4 were determined by real-time Q-RT-PCR method during adipogenic differentiation of MSC.RESULTS:The serum containing active components inhibited the adipogenic differentiation of MSC significantly.The expressions of PPARγ2 and FABP4 were significantly decreased after 3 and 6 days of adipogenesis induction.CONCLUSION:Zishen jiangtang pills can inhibit adipogenic differentiation of MSC,which might related to the expressions inhibition of PPARγ2 and FABP4.
Evaluating Biosignatures for Life Detection

Conceptual frameworks are developed for evaluating the ability of different biosignatures to provide evidence for the presence of life in planned missions or observational studies. The focus is on intrinsic characteristics of biosignatures in space environments rather than on their detection, which depends on technology. Evaluation procedures are drawn from extensive studies in decision theory on related problems in business, engineering, medical fields, and the social arena. Three approaches are particularly useful. Two of them, Signal Detection Theory and Bayesian hypothesis testing, are based on probabilities. The third approach is based on utility theory. In all the frameworks, knowledge about a subject matter has to be translated into probabilities and/or utilities in a multistep process called elicitation. We present the first attempt to cover all steps, from acquiring knowledge about biosignatures to assigning probabilities or utilities to global quantities, such as false positives and false negatives. Since elicitation involves human judgment that is always prone to perceptual and cognitive biases, the relevant biases are discussed and illustrated in examples. We further discuss at which stage of elicitation human judgment should be involved to ensure the most reliable outcomes. An example, how evaluating biosignatures might be implemented, is given in the Supplementary Information.
new species of Otopheidomenis (Acari : Otopheidomenidae) ectoparasitic on Ascalapha odorata (Lepidoptera : Noctuidae) in the Hawaiian Islands, with a key to the species in the genus

Otopheidomenis ascalaphae is described as new from specimens taken from a noctuid moth, Ascalapha odorata (Linnaeus), collected on Oahu I, Hawaiian Islands. A key to the species of Otopheidomenis is given. Treat (1955) proposed the family Otopheidomenidae and the genus Otopheidomenis to accommodate a single species, 0. zalelestes, collected from a noctuid moth. Sub­ sequently, 8 additional species of Otopheidomenis were described by Prasad (1968, 1970a, 1970b, 1972, 1973), only 1 of which, 0. treati (Prasad, 1968), was reported from a noctuid. The remaining 7 species were all described from sphingid moths. With the description of the new species below, the number of species in the genus is raised to 10 and a 3rd species is described from a noctuid. The holotype is in the collection ofthe B.P. Bishop Museum (BPBM) and paratypes are there and in the collections of the U.S. National Museum of Natural History/ Smithsonian Institution and the Acarology Laboratory, Ohio State University, Co­ lumbus. All measurements are given in micrometres. Chaetotaxy follows Evans 8c Till (1965). Otopheidomenis ascalaphae Syed 8c Goff, new species Fig. 1, 2 Diagnosis. Female and 6 with dorsal shield entire, without mediolateral incisions (other species having either mediolateral incisions or 2 dorsal shields); setae i3 absent (present in 0. oocytes Prasad, O. donahuei Prasad, and 0. indicus Prasad); 9 with 14 and 8 with 13 pairs of setae on dorsum of idiosoma, 2 pairs inserted on integument, remaining setae on shield; tritosternum reduced, setiform (absent in all other species except 0. zalelestes where it is reduced and oval; 9 with 1 pair of genital setae (absent in 0. indicus, 0. donahuei, 0. kayosiekeri Prasad, and 0. achlora Prasad); sternal shield small, convex anteriorly; 3 pairs of sternal setae inserted free on integument; 6 sternoventral shield with pointed lateral margins, bearing 4 pairs of setae; 2 with 2 spinose setae distoventrally on tarsi II-IV; femur II of 6 with a seta inserted on a distinct triangular posteroproximal protuberance (also seen in 0. indicus); tarsus IV with 4 elongate macrosetae.
MiR-483 induces senescence of human adipose-derived mesenchymal stem cells through IGF1 inhibition

Human adipose-derived mesenchymal stem cells (hADSCs) are an ideal source of seed cells for regenerative applications and tissue engineering. However, long-term in vitro culture of hADSCs reduces their quantity and quality, which lessens their value in research and clinical applications. The molecular mechanisms underlying this biological process are poorly defined. Recently identified microRNAs (miRNAs) have emerged as critical modulators of cellular senescence. In this study, we examined the changes in hADSCs undergoing senescence. Significant miR-483-3p upregulation was noted during in vitro passaging of hADSCs, which correlated with the adipogenic differentiation and cellular senescence. Knockdown of miR-483-3p retarded the adipogenic differentiation potential of hADSCs and reduced cellular senescence. Dual-luciferase reporter assays identified insulin-like growth factor-1 (IGF1) as the target gene of miR-483-3p. IGF1 inhibition confirmed its inhibitory effects on replicative senescence in hADSCs. In conclusion, our study revealed essential regulatory roles of miR-483-3p in the adipogenesis and aging of hADSCs mediated by targeting IGF1.
CRISPR interference of nucleotide biosynthesis improves production of a single‐domain antibody in Escherichia coli

Growth decoupling can be used to optimize the production of biochemicals and proteins in cell factories. Inhibition of excess biomass formation allows for carbon to be utilized efficiently for product formation instead of growth, resulting in increased product yields and titers. Here, we used CRISPR interference to increase the production of a single‐domain antibody (sdAb) by inhibiting growth during production. First, we screened 21 sgRNA targets in the purine and pyrimidine biosynthesis pathways and found that the repression of 11 pathway genes led to the increased green fluorescent protein production and decreased growth. The sgRNA targets pyrF, pyrG, and cmk were selected and further used to improve the production of two versions of an expression‐optimized sdAb. Proteomics analysis of the sdAb‐producing pyrF, pyrG, and cmk growth decoupling strains showed significantly decreased RpoS levels and an increase of ribosome‐associated proteins, indicating that the growth decoupling strains do not enter stationary phase and maintain their capacity for protein synthesis upon growth inhibition. Finally, sdAb production was scaled up to shake‐flask fermentation where the product yield was improved 2.6‐fold compared to the control strain with no sgRNA target sequence. An sdAb content of 14.6% was reached in the best‐performing pyrG growth decoupling strain.
Prevalence of and Risk Factors for Campylobacter spp. Colonization of Broiler Chicken Flocks in Greece.

The prevalence and risk factors for Campylobacter spp. colonization of broiler flocks and broiler carcass contamination in Greek slaughterhouses were investigated. Over a 14-month period, a pool of 10 ceca and 5 neck skin samples from chicken carcasses were collected from each of 142 batches of broiler flocks slaughtered in 3 different slaughterhouses. Information on potential risk factors for Campylobacter infection in broilers was collected by an on-farm interview and linked according to the Campylobacter contamination status of broiler flocks and differences in farm characteristics and management practices identified from questionnaires. Campylobacter spp. was isolated from 73.94% and 70.42% of ceca (95% CI 65.92-80.94) and carcasses (95% CI 62.19-77.78), respectively. A significant correlation (p < 0.001) between the presence of Campylobacter spp. in broiler ceca and contamination of carcasses was found, suggesting the spread of the microorganism on the skin of carcasses during the slaughtering procedure. A multiple logistic regression showed the disinfection of the poultry house being conducted by unskilled personnel (odds ratio [OR] ¼ = 3.983) as a significant risk factor (p < 0.05) and the use of straw litter as bedding material (OR ¼ = 0.170) and closure of windows during the intervals of production cycles (OR ¼ = 0.396) as significant protective factors (p < 0.05) for broiler flock contamination. These results are important and help further the understanding of the epidemiology of Campylobacter spp. derived from poultry in Greece.
The Body Temperature Rhythm of the Thirteen-Lined Ground Squirrel, Spermophilus tridecemlineatus

The circadian rhythm of body temperature of the thirteen-lined ground squirrel, Spermophilus tridecemlineatus, was studied by telemetry. The amplitude of the daily variation of body temperature in squirrels maintained under a long photoperiod (14L:10D) at a neutral ambient temperature (24°C) was found to be much larger than that of most endotherms (range ~34° - 39°C). Four out of nine animals entered daily (or multidaily) torpor with body temperatures approaching ambient temperature. The body temperature of eight out of nine animals was severely reduced by exposure to lower environmental temperatures (down to 5°C). In free-running conditions, as well as in the entrained state, the body temperature rhythm remained synchronized to the rhythm of locomotor activity.
Comportamiento térmico-energético de tipologías representativas pertenecientes a mosaicos urbanos de la ciudad de La Plata

RESUMEN: Se plantea profundizar el estudio del comportamient o de áreas urbanas homogéneas del partido de La Pla ta a partir de sectores urbanos reducidos entendidos com o Mosaicos Urbanos, los cuales presentan caracterís ticas homogéneas respecto al uso y ocupación del suelo, a la densida d poblacional, los servicios básicos de infraestruc tura y a la diversidad tipológica. Como ejemplo se plantea el estudio del c omportamiento térmico-energético de tres tipologías residenciales representativas: vivienda compacta; Vivienda compac ta en “Duplex”; y Vivienda poco compacta en “L”. Es ta representan respectivamente el 67 %, el 9,5 % y el 12,7 % de la s tipologías encontradas en un Mosaico Urbano de Co nsolidación Media. En esta etapa, se plantea analizar las siguientes v ariables de comportamiento térmico-energético i. Al cance de niveles de confort utilizando energía convencional; ii. Alcanc e de niveles de confort sin incorporación de energ ía auxiliar; y iii. Consumo Energético. Los resultados obtenidos permiti rán construir indicadores de referencia para toda e l área de análisis, aportando a las propuestas para su mejoramiento.
The Middle Triassic Megafossil Flora of the Basin Creek Formation, Nymboida Coal Measures, New South Wales, Australia. Part 3. Fern-like Foliage

Two quarries in the Basin Creek Formation of the Middle Triassic Nymboida Coal Measures have yielded numerous examples of fern-like foliage. No affiliated fertile material is available to place the fronds in a natural classification. Twenty three species in twelve genera are described as morpho-taxa in Order and Family Incertae Sedis. Plants described in this paper are:- Cladophlebis conferta sp. nov., C. octonerva sp. nov., C. paucinerva sp. nov., C. retallackii sp. nov., C. sinuata sp. nov., C. tenuipinnula sp. nov., Dictyonymba sparnosa gen. et sp. nov., Gouldianum alethopteroides gen. et sp. nov., Leconama stachyophylla gen. et sp. nov., Micronymbopteris repens gen. et sp. nov., Nymbiella lacerata gen. et sp. nov., Nymboidiantum glossophyllum (Tenison-Woods) gen. et comb. nov., N. multilobatum gen. et sp. nov., N. elegans gen. et sp. nov., N. fractiflexum gen. et sp. nov., N. robustum gen. et sp. nov., Nymbophlebis polymorpha gen. et sp. nov., Nymbopteron dejerseyi (Retallack) gen. et comb. nov., N. foleyi gen. et sp. nov., N. uncinatum gen. et sp. nov., Nymborhipteris radiata gen. et sp. nov., Ptilotonymba curvinervia gen. et sp. nov. and Sphenopteris speciosa sp. nov. The diversity of this new material demonstrates the remarkable recovery of Gondwana vegetation following the end-Permian extinction event.
Determination of the sensitivity and specificity of PCR by using different gene primers to detect Helicobacter pylori in gastric biopsy specimens

Introduction Almost half of the world's population undergoes from the Helicobacter pylori (H. pylori) infection. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma. several invasive and noninvasive tests are currently available for diagnosis of H.pylori. Methods based on molecular biology are considered highly specific and sensitive tests, and many PCR-based assays have been developed to detect H.pylori DNA in gastric biopsies. However, this technique is able to detects specific fragments but not viable bacteria, and its sensitivity also depends on several factors. The aim of this study is to determine the sensitivity and specificity of PCR primers to diagnose Helicobacter pylori infection. Method One hundred H. pylori strains will isolate from patients with different gastrointestinal disease who referred to Tabriz Emam Reza hospital. Any sample positive on histological examination as well as rapid urease test was considered as the gold standard for determination of the sensitivity and specificity of the PCR methods. ResultsPreliminary results are provided and the final results will be presented in Congress. Genomic DNAs were extracted from all strains . 20 sample was examined by different PCRs. ConclusionsDiscussion after obtaining the final results will be presented in Congress.
Pathogenic Bacteria Causing Ascites Disease of Cultured Yellow Catfish(Pelteobagrus fulvidraco)

In recent years,a great number of yellow catfish (Pelteobagrus fulvidraco)were sick and finally died,in the fishery ponds of Jiangsu province,with the distinctness symptom of belly tumidity,blood in the liver and body surface,no food intake,and soliquoid.In this paper,a dominant bacterial was purified from the ascites,liver,gill and canker skin of the sick yellow fish from several farms.With the help of light microscope,TEM,biochemical characteristics of the bacterial,and the molecular systematic (phylogenetic tree based on 16S rDNA sequences),the bacterial was identified.It was concluded that the bacterial isolated from the sick yellow fish above,was Edwardsiella tarda,which induced the serious Hemorrhagic Sapticemia disease in the fishery ponds in Jiangsu before,with the homology of 99%,and together with Edwardsiella tarda in phylogenetic tree,what's more,with the same biochemical characteristics as Edwardsiella tarda.It was the first report about the prevalence of Edwardsiella tarda in yellow catfish.
EFEITO DA ESTAÇÃO DE NASCIMENTO SOBRE A FREQUÊNCIA DE BEZERRAS SOROPOSITIVAS PARA Anaplasma marginale E Babesia bovis NA REGIÃO SUL DE MINAS GERAIS, BRASIL

The objective of this study was to determine the frequency of natural infections by Anaplasma marginale and Babesia bovis in female dairy calves been in the dry (April the August) and rainy seasons (September the March), in two dairy farms (Limeira and Bocaina) located in the Lavras municipality, Southern Region of the Minas Gerais State, Brazil. Were performed blood smears and serology by mean of the test of indirect fluorescent antibody test (IFAT). In the determination of the primary infection was observed a average of 60.8 days of age for A. marginale. In the Limeira farm there was no significant influence of birth season on the average of age in calves infected for A. marginale (p>0.05). For the Bocaina farm the average of age for of the primary infection for A. marginale was higher in the calves born in the dry season (p KEY WORDS: Anaplasmosis, babesiosis, cattle, indirect fluorescent antibody test.