Miracle-dz/UniFAR-FTUs huggingface dataset:
β‐endorphin differentially contributes to food anticipatory activity in male and female mice undergoing activity‐based anorexia

Anorexia nervosa (AN) has a lifetime prevalence of up to 4% and a high mortality rate (~5–10%), yet little is known regarding the etiology of this disease. In an attempt to fill the gaps in knowledge, activity‐based anorexia (ABA) in rodents has been a widely used model as it mimics several key features of AN including severely restricted food intake and excessive exercise. Using this model, a role for the hypothalamic proopiomelanocortin (POMC) system has been implicated in the development of ABA as Pomc mRNA is elevated in female rats undergoing the ABA paradigm. Since the Pomc gene product α‐MSH potently inhibits food intake, it could be that elevated α‐MSH might promote ABA. However, the α‐MSH receptor antagonist SHU9119 does not protect against the development of ABA. Interestingly, it has also been shown that female mice lacking the mu opioid receptor (MOR), the primary receptor activated by the Pomc‐gene‐derived opioid β‐endorphin, display blunted food anticipatory behavior (FAA), a key feature of ABA. Thus, we hypothesized that the elevation in Pomc mRNA observed during ABA may lead to increased β‐endorphin concentrations and MOR activation to promote ABA. Further, given the known sex differences in AN and ABA, we hypothesized that MORs may contribute differentially in male and female mice. Using wild‐type and MOR knockout mice of both sexes, a MOR antagonist and careful analysis of food anticipatory behavior and β‐endorphin levels, we found 1) increased Pomc mRNA levels in both female and male mice that underwent ABA, 2) increased β‐endorphin in female mice that underwent ABA, and 3) blunted FAA in both sexes in response to MOR genetic deletion yet blunted FAA only in males in response to MOR antagonism. The results presented provide support for both hypotheses and suggest that it may be the β‐endorphin resulting from increased Pomc transcription that supports the development of some features of ABA.
Evaluation Strategies for Triple‐Drug Combinations against Carbapenemase‐Producing Klebsiella Pneumoniae in an In Vitro Hollow‐Fiber Infection Model

Mounting antimicrobial resistance to carbapenemase‐producing Klebsiella pneumoniae (CPKP) highlights the need to optimize currently available treatment options. The objective of this study was to explore alternative dosing strategies that limit the emergence of resistance to preserve the utility of last‐line antibiotics by: (i) evaluating the pharmacodynamic (PD) killing activity of simulated humanized exposures to monotherapy and two‐drug and three‐drug combinations against CPKP bacterial isolates with different resistance mechanisms; and (ii) optimizing polymyxin B (PMB) exposure simulated in the three‐drug combination regimens to maximize the killing activity. Two CPKP clinical isolates (BAA2146 (NDM‐1) and BRKP76 (KPC‐2)) were evaluated over 168 hours using a hollow‐fiber infection model simulating clinically relevant PMB, fosfomycin, and meropenem dosing regimens. PMB‐based three‐drug combinations were further optimized by varying the initial exposure (0–24 hours) or maintenance dose received over the duration of treatment. The area under the bacterial load‐versus‐time curve (AUCFU) was used to determine PD activity. Overall reductions in PMB exposure ranged from 2 to 84%. BAA2146 and BRKP76 had median (range) AUCFUs of 11.0 (10.6–11.6) log10 CFU hour/mL and 7.08 (7.04–11.9) log10 CFU hour/mL, respectively. The PMB “front loaded” 2.5 mg/kg/day + 0.5 mg/kg maintenance dose in combination with meropenem and fosfomycin was a promising regimen against BRKP76, with an overall reduction in PMB exposure of 56% while still eradicating the bacteria. Tailored triple‐combination therapy allows for the optimization of dose and treatment duration of last‐line agents like PMB to achieve adequate drug exposure and appropriate PD activity while minimizing the emergence of resistance.
Suction above cuff endotracheal tube can reduce ventilator-associated pneumonia in COVID-19 patients.

97 www.journals. viamedica.pl Address for correspondence: Łukasz Szarpak, Maria Sklodowska-Curie Bialystok Oncology Center, Bialystok, Poland; e-mail: lukasz.szarpak@gmail.com DOI: 10.5603/ARM.a2021. 0025 Received: 14.11. 2020 Copyright © 2021 PTChP ISSN 2451–4934 Łukasz Szarpak, Maciej Cyran, Paweł Wieczorek, Togay Evrin Maria Sklodowska-Curie Bialystok Oncology Center, Bialystok, Poland Maria Sklodowska-Curie Medical Academy in Warsaw, Poland Polish Society of Disaster Medicine, Warsaw, Poland Department of Emergency Medicine, Ufuk University Medical Faculty, Dr Ridvan Ege Education and Research Hospital, Ankara, Turkey
Quantitative Analysis of Mobile Proteins in Normal Brain Tissue by Amide Proton Transfer Imaging: Age Dependence and Sex Differences

Purpose The aims of this study were to evaluate the relationship between age change and amide proton transfer (APT) signal in each region of the whole brain and to derive the standard value of APT signal in each brain region of normal adults. Materials and Methods Using the mDIXON 3-dimensional–APT sequence of the fast spin echo method, an APT image was obtained. In total, 60 patients (mean age, 49.8 ± 16.9 years) with no abnormal findings on magnetic resonance imaging data were included. For image analysis, registration parameters were created using the FMRIB Software Library 5.0. 11, and then a region of interest was set in the Montreal Neurological Institute structural atlas for analysis. Statistical analyses were performed using the age-dependent and sex differences in APT signals from each brain region. Results No significant correlation was seen between APT signal and age and sex in all brain regions. Conclusion Under the APT imaging parameter conditions used in this study, local brain APT signals in healthy adults are independent of age and sex.
Four years of low dose enzalutamide for metastatic castration-resistant prostate cancer.

BACKGROUND The management of metastatic castration-resistant prostate cancer involves second-generation antiandrogen therapy, including enzalutamide. The recommended dose of 160mg/day is sometimes difficult to use in elderly patients because of the burden of comorbidities. The usefulness of a low effective dose remains unclear. METHODS We report the first ever patient, now aged 87 years, who had progressing prostate cancer and was treated at 25% of the recommended dose for over 4 years. RESULTS The most striking observation is that nothing extraordinary happened. The disease responded without dramatic toxicities. His follow-up has been serene on both aspects of prostate cancer and general health. CONCLUSION Cautious dosage can have a long-lasting favorable impact in the elderly patient.
RNA tape sampling in cutaneous lupus erythematosus discriminates affected from unaffected and healthy volunteer skin

Objective Punch biopsy, a standard diagnostic procedure for patients with cutaneous lupus erythematosus (CLE) carries an infection risk, is invasive, uncomfortable and potentially scarring, and impedes patient recruitment in clinical trials. Non-invasive tape sampling is an alternative that could enable serial evaluation of specific lesions. This cross-sectional pilot research study evaluated the use of a non-invasive adhesive tape device to collect messenger RNA (mRNA) from the skin surface of participants with CLE and healthy volunteers (HVs) and investigated its feasibility to detect biologically meaningful differences between samples collected from participants with CLE and samples from HVs. Methods Affected and unaffected skin tape samples and simultaneous punch biopsies were collected from 10 participants with CLE. Unaffected skin tape and punch biopsies were collected from 10 HVs. Paired samples were tested using quantitative PCR for a candidate immune gene panel and semi-quantitative immunohistochemistry for hallmark CLE proteins. Results mRNA collected using the tape device was of sufficient quality for amplification of 94 candidate immune genes. Among these, we found an interferon (IFN)-dominant gene cluster that differentiated CLE-affected from HV (23-fold change; p<0.001) and CLE-unaffected skin (sevenfold change; p=0.002), respectively. We found a CLE-associated gene cluster that differentiated CLE-affected from HV (fourfold change; p=0.005) and CLE-unaffected skin (fourfold change; p=0.012), respectively. Spearman’s correlation between per cent area myxovirus 1 protein immunoreactivity and IFN-dominant mRNA gene cluster expression was highly significant (dermis, rho=0.86, p<0.001). In total, skin tape-derived RNA expression comprising both IFN-dominant and CLE-associated gene clusters correlated with per cent area immunoreactivity of some hallmark CLE-associated proteins in punch biopsies from the same lesions. Conclusions A non-invasive tape RNA collection technique is a potential tool for repeated skin biomarker measures throughout a clinical trial.
Associations with antibiotic prescribing for acute exacerbation of COPD in primary care: secondary analysis of a randomised controlled trial

Background C-reactive protein (CRP) point-of-care testing can reduce antibiotic use in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) in primary care, without compromising patient care. Further safe reductions may be possible. Aim To investigate the associations between presenting features and antibiotic prescribing in patients with AECOPD in primary care. Design and setting Secondary analysis of a randomised controlled trial of participants presenting with AECOPD in primary care (the PACE trial). Method Clinicians collected participants’ demographic features, comorbid illnesses, clinical signs, and symptoms. Antibiotic prescribing decisions were made after participants were randomised to receive a point-of-care CRP measurement or usual care. Multivariable regression models were fitted to explore the association between patient and clinical features and antibiotic prescribing, and extended to further explore any interactions with CRP measurement category (CRP not measured, CRP <20 mg/l, or CRP ≥20 mg/l). Results A total of 649 participants from 86 general practices across England and Wales were included. Odds of antibiotic prescribing were higher in the presence of clinician-recorded crackles (adjusted odds ratio [AOR] = 5.22, 95% confidence interval [CI] = 3.24 to 8.41), wheeze (AOR = 1.64, 95% CI = 1.07 to 2.52), diminished vesicular breathing (AOR = 2.95, 95% CI = 1.70 to 5.10), or clinician-reported evidence of consolidation (AOR = 34.40, 95% CI = 2.84 to 417.27). Increased age was associated with lower odds of antibiotic prescribing (AOR per additional year increase = 0.98, 95% CI = 0.95 to 1.00), as was the presence of heart failure (AOR = 0.32, 95% CI = 0.12 to 0.85). Conclusion Several demographic features and clinical signs and symptoms are associated with antibiotic prescribing in AECOPD. Diagnostic and prognostic value of these features may help identify further safe reductions.
Long term follow‐up results of ablation treatment for patients with small renal mass

The aim of this study is to evaluate the outcome of ablation therapy in our clinic for the treatment of patients with a small renal mass Materials and Methods: We retrospectively evaluated the technic and follow‐up data of 30 patients with 36 tumours who underwent Radiofrequency Ablation (RFA) and Microwave Ablation (MWA) in our clinic. Demographic data, ablation type, tumour characteristics, peroperative and postoperative complications and treatment success of the patients were evaluated.
The relationship between loneliness, depression, internet and social media addiction among young Polish women.

OBJECTIVE When speaking of behavioral addictions (especially to the Internet and social media), it is emphasized that it is not the environment that is the main contributor to addiction, but rather certain behaviors and personality traits. The aim of this study was to assess the level of Internet and social media addiction on the example of Facebook with regard to psychological and social factors. SUBJECTS AND METHODS This survey-based study involved a group of women representing the female population in the West Pomeranian Voivodeship, Poland (N = 556). Research instruments were a self-developed questionnaire concerning sociodemographic data, the De Jong Gierveld Loneliness Scale, the Beck Depression Inventory, the Internet Addiction Test, and the Bergen Facebook Addiction Scale. RESULTS Age, depressive symptoms, loneliness were the variable contributing to Internet and Facebook addiction among the studied. Available studies confirm the results of their own research. CONCLUSIONS Depressive symptoms and dependence on the Internet and Facebook were more common among single women. In the employed women, we only observed higher levels of Internet and Facebook addiction. The level of dependence on the Internet and Facebook was higher among younger women. Loneliness correlated with Internet and Facebook addiction, and more severe depressive symptoms entailed higher levels of Internet and Facebook addiction.
Feasibility of Deep Learning-Guided Attenuation and Scatter Correction of Whole-Body 68Ga-PSMA PET Studies in the Image Domain

Objective This study evaluates the feasibility of direct scatter and attenuation correction of whole-body 68Ga-PSMA PET images in the image domain using deep learning. Methods Whole-body 68Ga-PSMA PET images of 399 subjects were used to train a residual deep learning model, taking PET non–attenuation-corrected images (PET-nonAC) as input and CT-based attenuation-corrected PET images (PET-CTAC) as target (reference). Forty-six whole-body 68Ga-PSMA PET images were used as an independent validation dataset. For validation, synthetic deep learning–based attenuation-corrected PET images were assessed considering the corresponding PET-CTAC images as reference. The evaluation metrics included the mean absolute error (MAE) of the SUV, peak signal-to-noise ratio, and structural similarity index (SSIM) in the whole body, as well as in different regions of the body, namely, head and neck, chest, and abdomen and pelvis. Results The deep learning–guided direct attenuation and scatter correction produced images of comparable visual quality to PET-CTAC images. It achieved an MAE, relative error (RE%), SSIM, and peak signal-to-noise ratio of 0.91 ± 0.29 (SUV), −2.46% ± 10.10%, 0.973 ± 0.034, and 48.171 ± 2.964, respectively, within whole-body images of the independent external validation dataset. The largest RE% was observed in the head and neck region (−5.62% ± 11.73%), although this region exhibited the highest value of SSIM metric (0.982 ± 0.024). The MAE (SUV) and RE% within the different regions of the body were less than 2.0% and 6%, respectively, indicating acceptable performance of the deep learning model. Conclusions This work demonstrated the feasibility of direct attenuation and scatter correction of whole-body 68Ga-PSMA PET images in the image domain using deep learning with clinically tolerable errors. The technique has the potential of performing attenuation correction on stand-alone PET or PET/MRI systems.
Positive Emotion Dysregulation Identifies Trauma-Exposed Community Individuals at Risk for Suicide and Nonsuicidal Self-Injury

Abstract Emotion dysregulation is associated with increased risk for suicidal thoughts and behaviors (STBs) and nonsuicidal self-injury (NSSI). However, research in this area has focused almost exclusively on dysregulation stemming from negative emotions. The present study aimed to address this gap in the literature by examining the associations between the specific domains of positive emotion dysregulation and both STBs and NSSI. Participants included 397 trauma-exposed community adults (Mage = 35.95; 57.7% female; 76.8% White). Results demonstrated significant associations between positive emotion dysregulation and both STBs and NSSI. In particular, higher levels of nonacceptance of positive emotions were found to be significantly related to risk for STBs (versus no risk), higher severity of STBs, and history of NSSI (versus no history). Findings suggest positive emotion dysregulation may play an important role in the etiology and treatment of both STBs and NSSI among trauma-exposed individuals.
Quantitative study showing how and why students access lecture recordings, and of the association between accessing and academic outcomes, in a biochemistry course

Many factors predict academic performance at university but accessing lecture recordings has not been established as one. Our aims were to quantify how and why biochemistry students accessed lecture recordings and to determine any association between accessing lecture recordings and academic outcomes. Lecture recording access data was collected and related to academic outcomes. On average, students accessed 46% of lecture recordings and each recording was accessed 2.3 times, but not to completion. After lecture delivery, students were slow to access lecture recordings, and the number of accesses was still increasing prior to the examinations. For the entire cohort, there were weak positive associations between accessing lecture recordings and academic outcomes for the semester, and between the access to lecture recordings in weeks 1–5 and 6–11 with the mid‐semester and final examination, respectively. For programs, the weak association for the semester was only apparent for the pharmacy and biomedical science students, and not for the medical laboratory or nutrition students. The most common reason for accessing lecture recording was to “Revise lecture concepts for assessment purposes. ” For students, who did not attend lectures, lecture recordings were accessed predominantly as “I prefer the flexibility of online recordings. ” Flexibility was also the theme of the positive additional feedback on accessing lecture recordings. In conclusion, consideration needs to be given on how to persuade students to access lecture recordings in a timelier way. As accessing lecture recordings is weakly associated with positive academic outcomes for some program cohorts, this supports their continued availability.
Hydrocyclones for De-oiling Applications—A Review

Abstract The de-oiling hydrocyclone is a device for liquid–liquid separation and production water cleanup. Significant progress has been made in the development of such a device since its first introduction and use in the late 1970s. The present article is a literature review of development and research work performed so far on de-oiling hydrocyclones. It reviews the performance parameters affecting the de-oiling hydrocyclone operation; namely, the inlet oil concentration and drop size distribution, the turn-down ratio, the pressure drop ratio, the flow split, and the geometrical parameters. The article addresses work done to elucidate the internal flow structure and performance of de-oiling hydrocylones in both experimental and computational fluid dynamics areas. Challenges and remaining research issues are also identified.
Non–line-of-sight imaging over 1.43 km

Significance Non–line-of-sight (NLOS) imaging can recover details of a hidden scene from the indirect light that has scattered multiple times. Despite recent advances, NLOS imaging has remained at short-range verifications. Here, both experimental and conceptual innovations yield hardware and software solutions to increase NLOS imaging from meter to kilometer range. This range is about three orders of magnitude longer than previous experiments. The results will open avenues for the development of NLOS imaging techniques and relevant applications to real-world conditions. Non–line-of-sight (NLOS) imaging has the ability to reconstruct hidden objects from indirect light paths that scatter multiple times in the surrounding environment, which is of considerable interest in a wide range of applications. Whereas conventional imaging involves direct line-of-sight light transport to recover the visible objects, NLOS imaging aims to reconstruct the hidden objects from the indirect light paths that scatter multiple times, typically using the information encoded in the time-of-flight of scattered photons. Despite recent advances, NLOS imaging has remained at short-range realizations, limited by the heavy loss and the spatial mixing due to the multiple diffuse reflections. Here, both experimental and conceptual innovations yield hardware and software solutions to increase the standoff distance of NLOS imaging from meter to kilometer range, which is about three orders of magnitude longer than previous experiments. In hardware, we develop a high-efficiency, low-noise NLOS imaging system at near-infrared wavelength based on a dual-telescope confocal optical design. In software, we adopt a convex optimizer, equipped with a tailored spatial–temporal kernel expressed using three-dimensional matrix, to mitigate the effect of the spatial–temporal broadening over long standoffs. Together, these enable our demonstration of NLOS imaging and real-time tracking of hidden objects over a distance of 1.43 km. The results will open venues for the development of NLOS imaging techniques and relevant applications to real-world conditions.
An investigation into the quality of wine: Evidence from piedmont

Abstract This paper studies a combination of four quality ratings as dependent variables, regressing them on a large number of explanatory variables. In particular, the focus is on Piedmont, which is the largest and the most important Italian wine region; it produces prestigious wines such as great Barolo and Barbaresco as well as lower-quality products. The data gathered enable us to test the effect on different measures of quality of a series of wine- and winery-dependent factors. In particular, a wide range of factors, which include classification, appellation, individual reputation, ownership structure and owner preferences, have been tested.
Understanding the Barriers of HIV Testing Services for Men Who Have Sex With Men and Transgender Women in Bangladesh: A Qualitative Study

Coverage of HIV testing services (HTS) is generally low among men who have sex with men (MSM) and transgender women (hijra) in Bangladesh, thus impeding the national goal of attaining the 90-90-90 target. In this context, this article delineates HTS uptake barriers among these populations. This qualitative study entailed 30 in-depth interviews, six focus groups and seven key-informant interviews with purposively selected MSM and hijra, alongside service providers. Participants cited individual and interpersonal barriers such as low risk perception and misconceptions about HIV testing, programmatic barriers such as knowledge gaps among peer service providers, as well as community and structural barriers such as the criminalization and stigmatization of male-to-male sex. Considering these contexts, it is essential for stakeholders to improve the HTS modality using multipronged approaches to address the multifaceted barriers of HTS uptake.
Broad band tuning of the plasmonic resonance of gold nanoparticles hosted in self-organized soft materials

The extraordinary properties of reconfigurable soft materials are used to drive the resonance properties of noble metal nanoparticles, using an approach that puts a bridge between soft matter and plasmonics. Gold nanoparticles have been dissolved in a cholesteric liquid crystal and then infiltrated in a micro-periodic polymeric structure, realized by combining a holographic step and a microfluidic etching process. The spectral behavior of the nano-composite soft-structure has been investigated in the UV-Vis range for two different polarization directions of the impinging probe light. Correlation between the optical response and external perturbations (electric field, temperature variation) gives an outstanding example of broadband tuning of an “active” plasmon resonance.