Miracle-dz/UniFAR-FTUs huggingface dataset:
Variability of Foveal Avascular Zone Metrics Derived From Optical Coherence Tomography Angiography Images

Purpose To characterize sources of inter- and intrasubject variability in quantitative foveal avascular zone (FAZ) metrics. Methods Two 3×3-mm optical coherence tomography angiography scans (centered on the fovea) were acquired in both eyes of 175 subjects. An image of the superficial plexus was extracted from each scan and segmented twice by a single observer. Four quantitative FAZ morphology metrics (area, axis ratio, acircularity, major horizontal axis angle) were calculated, and a variance components analysis was performed. Results Mean (±SD) age was 27.9 ± 11.9 years, and 55% were female. Area had the largest amount of variance resulting from intersubject differences (93.1%). In contrast, there was large interocular variance for axis ratio, acircularity, and major horizontal axis angle (55.0%, 53.7%, 70.7%, respectively), though only axis ratio showed significant asymmetry between fellow eyes (P < 0.05). Neither repeated images from the same eye nor repeated segmentation on the same image were significant sources of variance. Conclusions Metrics of FAZ morphology show excellent repeatability and reliability. Excluding FAZ area, there was a high amount of variance attributed to interocular differences for the other FAZ metrics; therefore, the fellow eye should not be considered a control for FAZ studies when using these metrics. Translational Relevance Vision scientists must be prudent when choosing FAZ metrics, as they display varying degrees of within-subject differences relative to between-subject differences. It seems likely that different metrics will be best suited for different tasks, such as monitoring small changes over time within a single subject or assessing whether a given FAZ is abnormal.
Raman characterizations of red blood cells with β-thalassemia using laser tweezers Raman spectroscopy

Abstract This study aimed to study the differences in Raman spectra of red blood cells (RBCs) among patients with &bgr;-thalassemia and controls using laser tweezers Raman spectroscopy (LTRS) system. A total of 33 patients with &bgr;-thalassemia major, 49 with &bgr;-thalassemia minor, and 65 controls were studied. Raman spectra of RBCs for each sample were recorded. Principal component analysis (PCA), one-way analysis of variance (ANOVA), and independent-sample t test were performed. The intensities of Raman spectra of &bgr;-thalassemia (major and minor) RBCs were lower than those of controls, especially at bands 1546, 1603, and 1619 cm–1. The intensity ratio of band 1546 cm–1 to band 1448 cm–1 demonstrated that there was a significant difference between the spectra of &bgr;-thalassemia major (mostly below 2.15) and those of controls. The spectra of controls could be well distinguished from those of &bgr;-thalassemia major using PCA. After normalization, the spectra of two different genotypes with &bgr;0/&bgr;0 mutations mainly overlapped, while those with &bgr;+/&bgr;+ mutations had lower intensity at bands 1546, 1603, and 1619 cm–1. The present study provided Raman characteristics of RBCs in patients with &bgr;-thalassemia major and supported the use of LTRS as a method for screening &bgr;-thalassemia major. The recognition rate for &bgr;-thalassemia minor needs to be further improved.
Sampling-based Estimation of In-degree Distribution with Applications to Directed Complex Networks

The focus of this work is on estimation of the in-degree distribution in directed networks from sampling network nodes or edges. A number of sampling schemes are considered, including random sampling with and without replacement, and several approaches based on random walks with possible jumps. When sampling nodes, it is assumed that only the out-edges of that node are visible, that is, the in-degree of that node is not observed. The suggested estimation of the in-degree distribution is based on two approaches. The inversion approach exploits the relation between the original and sample in-degree distributions, and can estimate the bulk of the in-degree distribution, but not the tail of the distribution. The tail of the in-degree distribution is estimated through an asymptotic approach, which itself has two versions: one assuming a power-law tail and the other for a tail of general form. The two estimation approaches are examined on synthetic and real networks, with good performance results, especially striking for the asymptotic approach.
Laitinen’s Subgenual Cingulotomy: Anatomical Location and Case Report

Background: The widespread use of deep brain stimulation (DBS) for movement disorders has renewed the interest in DBS for psychiatric disorders. Lauri Laitinen was a pioneer of stereotactic psychosurgery in the 1950s to 1970s, especially by introducing the subgenual cingulotomy. Our aim here was to verify the anatomical target used by Laitinen, to report on a patient who underwent this procedure, and to review the literature. Materials and Methods: The records of Helsinki University Hospital were searched for psychosurgical cases performed between 1970 and 1974. Alive consenting patients were interviewed and underwent a brain MRI. Results: We found 1 patient alive who underwent subgenual cingulotomy in 1971 for obsessive thoughts, anxiety, and compulsions, diagnosed at that time as “schizophrenia psychoneurotica. ” MRI showed bilateral subgenual cingulotomy lesions (254 and 160 mm3, respectively). The coordinates of the center of the lesions in relation to the midcommissural point for the right and left, respectively, were: 7.1 and 7.9 mm lateral; 0.2 mm inferior and 1.4 mm superior, and 33.0 and 33.9 anterior, confirming correct subgenual targeting. The patient reported retrospective satisfactory results. Conclusions: The lesion in this patient was found to be in the expected location, which gives some verification of the correct placement of Laitinen’s subgenus cingulotomy target.
Dietary Supplements and Cardiovascular Diseases

The market of nutritional supplements is expected to expand over 6%/year through 2018 due to growing interest in personal health, aging population, and promising personalized care products. The most used dietary supplements are fish oil, multivitamins, Vitamin D, and coenzyme Q10 (CoQ10) in this order, while probiotics is the fastest growing supplement. In the U.S., over 68% of the population use dietary supplements regularly. On the other hand, in the developed countries, cardiovascular diseases (CVDs) are the main cause of death and morbidity from the 1900s. The effects of most dietary supplements on cardiovascular risk and CVD have been studied for a long time. However, despite several studies explored the association of the various supplements to the cardiovascular risk, there is still a lack of consensus. Multivitamin supplementation has been advocated to reduce cardiovascular events; Vitamin D levels have been associated with the occurrence of coronary artery disease, heart failure, and atrial fibrillation; CoQ10 deficiency has been associated with myocardial dysfunction and with statin myopathy; probiotoics has been suggested to lower both blood pressure and circulating lipids. However, the study of the effects of dietary supplementations is not straightforward, since people assuming dietary supplements generally have a healthier diet and lifestyle, and randomized studies are rarely performed. In this review, we will summarize the findings linking dietary supplements to CVD with a special focus on novel insights.
Review of the prevalence of postnatal depression across cultures

The purpose of this review was to examine articles related to recent epidemiological evidence of the prevalence of maternal postnatal depression (PND) across different countries and cultures and to identify specific epidemiological studies that have been carried out exclusively in Malaysia on the prevalence of maternal PND. The review was undertaken in two stages, an initial review and an updated review. At both stages systematic literature searches of online databases were performed to identify articles on the prevalence of maternal PND. A total of 124 articles concerning research conducted in more than 50 countries were included in the final analysis. There were wide variations in the screening instruments and diagnostic tools used although the Edinburgh Postnatal Depression Scale (EPDS) was the most common instrument applied to identify PND. The prevalence of maternal PND ranged from 4.0% to 63.9%, with Japan and America recording the lowest and highest rates, respectively. Within continents, a wide variation in reported prevalence was also found. The reported rates of maternal PND in Malaysia were much higher than that previously documented with a range of 6.8–27.3%. This review indicated that the widely cited prevalence of maternal PND of 10–15% underestimates rates of PND worldwide. The reasons for this variability may not be fully explained by review methods. Future studies should evaluate the nature of women's PND experiences across cultures to explain these wide variations.
Is Internet overuse associated with impaired empathic ability in Korean college students?

Abstract The purpose of the present study was to determine whether Internet overuse was associated with empathic ability in college students. A total 261 participants (145 males and 116 females; mean age of 21.93 years) completed Empathy Quotient (EQ), Young's Internet Addiction Test (IAT), and questionnaires about demographic data and internet using patterns. Of these 261 subjects, 85 (32.5%) were categorized as over-users. There was no significant difference in EQ total score between the over-user group and the average user group. The over-user group had lower scores of Social Skills than the average user group in subscale profiles. The over-user group stayed longer in cyberspace than the average user group. EQ score was found to have positive correlation with time spent in Internet use and the number of close friends. Results of the present study suggest that conventional Internet use is positively associated with empathic ability in interpersonal relationships. Therefore, such positive association between Internet use and empathic ability should be considered when evaluating possible problematic internet use.
Different Originating Cells Underlie Intertumoral Heterogeneity in Lung Neuroendocrine Tumors.

Studies in genetically engineered mouse models of neuroendocrine lung cancer suggest that differences in cells of origin underlie subtype variations in this class of cancers. These findings highlight the concept that the same driver mutations introduced into different cells of origin lead to tumors with the same histology but dramatically different metastatic programs and potentially different therapeutic responses. Cancer Discov; 8(10); 1216-8. ©2018 AACR See related article by Yang et al., p. 1316.
Assessment of levels of awareness towards blood donation in Saudi Arabia

Background blood products is an interesting term as all blood and blood transfusion procedures carry risk even with advance screening of donors and donated blood. In Saudi Arabia voluntary donors are either the only source or not, the other source is paid donors and the lack of volunteers represents a major challenge. This is usually attributed to low community awareness regarding voluntary blood donation. Therefore, the objective of this study was to assess the levels of awareness towards blood donation in northern Saudi Arabia. Methodology in this descriptive cross sectional survey, data about blood donation were obtained from 717 Saudi volunteers living in the city of Hail, Saudi Arabia. A Purposeful questionnaire was designed and used for obtaining of the necessary data. Results When asking the participants to rate the level of awareness about blood donation in Saudi Society, the majority of participants categorized the levels as good followed by very good and poor, representing 48.8%, 29.3%, and 22% participants, respectively. Conclusion the broad concept of blood donation is still poor in Saudi Arabia. Further educational plans are needed to raise the level of awareness and increase the perception of blood donation among Saudi population. Efforts should be made to involve females in blood donation duties.
What Diplomacy in the Ancient near East Can Tell Us about Blockchain Technology

A blockchain is an institutional technology—a protocol—that allows for economic coordination between agents separated by boundaries of possible mistrust. Blockchains are not the only technology in history to have these characteristics. The paper looks at the role of the diplomatic protocol at the very beginning of human civilisation in the ancient near east. These two protocols—diplomatic and blockchain—have significant similarities. They were created to address to similar economic problems using similar mechanisms: a permanent record of past dealings, public and ritualistic verification of transactions, and game-theoretic mechanisms of reciprocity. The development of the diplomatic protocol allowed for the creation of the first international community and facilitated patterns of peaceful trade and exchange. Some questions about a generalised ‘protocol economics’ are drawn.
Helps from flipped classroom in learning suturing skill: The medical students’ perspective

Background Today, flipped classroom (FC) has been widely used in medical education. However, the effectiveness of FC remains controversial. The variation may cause by different subjects or different course design. Moreover, those studies did not explain how the association among different domains of learning objective was in FCs. The purpose of this study was to explore the help of learning domains from a FC of suturing skill in year-5 medical students. Design This study determined sample size according to statistical power. A minimum number of 77 participants for regression analysis are needed. Therefore, this study enrolled 78 medical students in a 2-hour suturing course, which consisted of pre-class video and in-class instruction. Both simple and mattress suturing were taught. The students received an anonymous survey with questionnaire of Help from Instruction Questionnaire for Clinical Skills (HIQ-CS) after the course. The HIQ-CS was developed by medical education team according to Bloom's taxonomy, and its reliability was favorable (Cronbach's ^l = 0.839). Factor loadings among all items in the HIQ-CS was also favorable (0.790 to 0.849). This study determined consensus of students' perspective by median (Me) and interquartile range (IQR), and tested mediation among different learning domains by regression. Results The results showed medical students agreed FC can help them in learning suturing (Me = 4, IQR = 1). The cognitive help (β = .526, p < .001) was completely mediated by psychomotor help (β = .399, p < .001) and affective help (β = .413, p < .001) to overall helps in FC. The affective help (β = .617, p < .001) was partially mediated by psychomotor help to overall helps in FC. Conclusions FC may help students in learning suturing skill in different domains. Our model explains the cognitive help from FC provides an important foundation for the helps of other domains. Although the model should be examined by different curricula and measurements in future, the model of help from instruction in our study provided an innovated concept and framework in medical education.
Growth and feeding of deep-sea coral Lophelia pertusa from the California margin under simulated ocean acidification conditions

The global decrease in seawater pH known as ocean acidification has important ecological consequences and is an imminent threat for numerous marine organisms. Even though the deep sea is generally considered to be a stable environment, it can be dynamic and vulnerable to anthropogenic disturbances including increasing temperature, deoxygenation, ocean acidification and pollution. Lophelia pertusa is among the better-studied cold-water corals but was only recently documented along the US West Coast, growing in acidified conditions. In the present study, coral fragments were collected at ∼300 m depth along the southern California margin and kept in recirculating tanks simulating conditions normally found in the natural environment for this species. At the collection site, waters exhibited persistently low pH and aragonite saturation states (Ωarag) with average values for pH of 7.66 ± 0.01 and Ωarag of 0.81 ± 0.07. In the laboratory, fragments were grown for three weeks in “favorable” pH/Ωarag of 7.9/1.47 (aragonite saturated) and “unfavorable” pH/Ωarag of 7.6/0.84 (aragonite undersaturated) conditions. There was a highly significant treatment effect (P < 0.001) with an average% net calcification for favorable conditions of 0.023 ± 0.009% d−1 and net dissolution of −0.010 ± 0.014% d-1 for unfavorable conditions. We did not find any treatment effect on feeding rates, which suggests that corals did not depress feeding in low pH/ Ωarag in an attempt to conserve energy. However, these results suggest that the suboptimal conditions for L. pertusa from the California margin could potentially threaten the persistence of this cold-water coral with negative consequences for the future stability of this already fragile ecosystem.
MYC‐driven malignant transformation of mature murine B cells requires inhibition of both intrinsic apoptosis and p53 activity

Increased expression of the oncogene MYC is a common feature of many B‐cell malignancies, however MYC overexpression by itself is not sufficient for transformation, and additional genetic events are required, although the exact nature of these remains unknown. In patients and in transgenic mouse models, oncogenic transformation may occur in B cells at various differentiation stages interacting with complex microenvironments. B‐cell oncogenesis often occurs after prolonged periods of time, making it difficult to accurately identify the genetic events required for transformation. An in vitro system, where malignant transformation of primary B cells could be analyzed, would facilitate the identification of genetic events required for transformation. Here, we describe such a system and show that primary murine B cells rapidly become transformed upon forced expression of MYC, in conjunction with simultaneous inhibition of the ARF/p53 axis via overexpression of BMI1, as well as through downregulation of p19ARF or expression of a dominant‐negative p53 and suppression of intrinsic apoptosis through overexpression of BCLXL or MCL1. Established tumor cells remained addicted to expression of the lymphoma‐inducing genes. In mice, transformed cells rapidly established fatal B‐cell lymphomas.
Color Processing in Zebrafish Retina

Zebrafish (Danio rerio) is a model organism for vertebrate developmental processes and, through a variety of mutant and transgenic lines, various diseases and their complications. Some of these diseases relate to proper function of the visual system. In the US, the National Eye Institute indicates >140 million people over the age of 40 have some form of visual impairment. The causes of the impairments range from refractive error to cataract, diabetic retinopathy and glaucoma, plus heritable diseases such as retinitis pigmentosa and color vision deficits. Most impairments directly affect the retina, the nervous tissue at the back of the eye. Zebrafish with long or short-wavelength color blindness, altered retinal anatomy due to hyperglycemia, high intraocular pressure, and reduced pigment epithelium are all used, and directly applicable, to study how these symptoms affect visual function. However, many published reports describe only molecular/anatomical/structural changes or behavioral deficits. Recent work in zebrafish has documented physiological responses of the different cell types to colored (spectral) light stimuli, indicating a complex level of information processing and color vision in this species. The purpose of this review article is to consolidate published morphological and physiological data from different cells to describe how zebrafish retina is capable of complex visual processing. This information is compared to findings in other vertebrates and relevance to disorders affecting color processing is discussed.
Anterior Fixation of Floating Facet Fractures in the Cervical Spine: A Prospective Case Series and Biomechanical Analysis

Abstract Background Unilateral fractures involving complete separation of the lateral mass from the vertebra and lamina (floating lateral mass fractures) are a unique subset of cervical spine fractures. These injuries are at significant risk for displacement without operative fixation. Posterior fixation has proven to facilitate adequate fusion. However, there are few data supporting the clinical success of single-level anterior fixation. Methods Biomechanical evaluation of floating lateral mass fractures and a consecutive case series of patients with rotationally unstable floating lateral mass fractures treated with anterior fixation using an integrated cage-screw device with anterior plating (ICSD) was performed. The study comprised 7 fresh human cadaver cervical spines (C2-C7), and 11 patients with floating lateral mass fractures. Segmental flexibility testing evaluating axial rotation, flexion/extension, and lateral bending was performed in a cadaveric model after 2 types of single-level anterior fixation and 1 type of 2-level posterior fixation. Eleven patients with a floating lateral mass fracture of the cervical spine underwent anterior fixation with an ICSD. Radiographs and clinical outcomes were retrospectively reviewed. Results Compared with the intact condition, posterior instrumentation significantly (P < .05) reduced range of motion (ROM) in all 3 planes; anterior fixation with cervical plate and interbody spacer significantly reduced ROM in lateral bending only; and the ICSD significantly reduced ROM in flexion/extension and lateral bending. In the clinical arm, there were no long-term complications, subsidence >2 mm, failure of fixation, reoperation, pseudoarthrosis, or listhesis at final follow-up. Conclusions The addition of 2 screws placed through a cervical cage can improve anterior fixation in a human cadaveric model of floating lateral mass fractures. Early clinical results demonstrate a low complication rate and a high rate of healing with single-level anterior fixation using this technique.
Roof model recommendation for complex buildings based on combination rules and symmetry features in footprints

ABSTRACT Currently, very few roof shape information for complex buildings is available on OSM. Moreover, additional data requirements (e.g. 3D point clouds) limit the applicability of many roof reconstruction approaches. To mitigate this issue, we propose an approach to roof shape recommendations for complex buildings by exploring the inherited characteristics of building footprints: the disclosure of rectangles combinations in a partition of footprints and the symmetrical features of footprints. First, it decomposes a complex footprint into rectangles by using an advanced minimal non-overlapping cover algorithm. Second, a graph-based symmetry detection algorithm is proposed to identify all the symmetrical sub-clusters in partitions. Then, a set of selection rules are defined to rank partitions, and the best ones are chosen for roof shape recommendation. Finally, a set of combination rules and a symmetry rule are defined. It enables to evaluate the probability of a footprint being a certain combination of roof shapes. Experimental results show the growth of the probability of correctly recommending roof shapes for single rectangles and buildings from a prior probability of 17–45% and from a prior probability of 0.29–14.3%, removing 60% and 93% of the incorrect roof shape options, respectively.