Miracle-dz/UniFAR-FTUs huggingface dataset:
Implication of nanoparticles/microparticles in mucosal vaccine delivery

Although polymeric nanoparticles/microparticles are well established for the mucosal administration of conventional drugs, they have not yet been developed commercially for vaccine delivery. The limitation of the mucosal (particularly oral) route of delivery, including low pH, gastric enzymes, rapid transit and poor absorption of large molecules, has made mucosal vaccine delivery challenging. Nevertheless, several polymeric delivery systems for mucosal vaccine delivery are currently being evaluated. The polymer-based approaches are designed to protect the antigen in the gut, to target the antigen to the gut-associated lymphoid tissue or to increase the residence time of the antigen in the gut through bioadhesion. M-cell targeting is a potential approach for mucosal vaccine delivery, which can be achieved using M-cell-specific lectins, microbial adhesins or immunoglobulins. While many hurdles must be overcome before targeted mucosal vaccine delivery becomes a practical reality, this is a potential area of research that has important implications for future vaccine development. This review comprises various aspects that could be decisive in the development of polymer based mucosal vaccine delivery systems.
Association between exercise and pubertal BMD is modulated by estrogen receptor alpha genotype.

UNLABELLED Genetic and environmental factors contribute to bone mass, but the ways they interact remain poorly understood. This study of 245 pre- and early pubertal girls found that the PvuII polymorphism in the ER-alpha gene modulates the effect of exercise on BMD at loaded bone sites. INTRODUCTION Impaired achievement of bone mass at puberty is an important risk factor for the development of osteoporosis in later life. Genetic, as well as environmental, factors contribute to bone mass, but the ways they interact with each other remain poorly understood. MATERIALS AND METHODS We investigated the interaction between a PvuII polymorphism at the ER-alpha gene and physical activity (PA) on the modulation of bone mass and geometry in 245 10- to 13-year-old pre- and early pubertal Finnish girls. Level of PA was assessed using a questionnaire. Bone properties were measured using DXA and pQCT. The analyses were controlled for the effects of Tanner stage and body size index. RESULTS Girls with heterozygote ER-alpha genotype (Pp) and high PA had significantly higher bone mass and BMD, as well as thicker cortex, at loaded bone sites than their low-PA counterparts. No differences were found in bone properties of the distal radius, which is not a weight-bearing bone. Bone properties did not differ in either homozygote groups (PP and pp) regardless of the PA level. CONCLUSIONS These findings suggest that the PvuII polymorphism in the ER-alpha gene may modulate the effect of exercise on BMD at loaded bone sites. The heterozygotes may benefit most from the effect of exercise, whereas neither of the homozygote groups received any significant improvement from high PA. Furthermore, high PA may hide the genetic influence on bone. Indeed, it seems that one may compensate one's less favorable Pp genotype by increasing leisure PA at early puberty.
Effect of everolimus on cell viability in nonfunctioning pituitary adenomas.

CONTEXT Pituitary adenomas can cause specific syndromes due to hormone excess and/or determine sellar mass symptoms. Pituitary cell growth can sometimes be influenced by medical therapy, such as for somatotroph adenomas treated with somatostatin analogs or prolactinomas treated with dopaminergic drugs. However, nonfunctioning pituitary adenomas (NFAs) are still orphans of medical therapy. Everolimus (RAD001), a derivative of rapamycin, is a well-known immunosuppressant drug, which has been recently shown to have antineoplastic activity in several human cancers. OBJECTIVE The objective of the study was to investigate the possible antiproliferative effects of RAD001 in human NFAs. DESIGN We collected 40 NFAs that were dispersed in primary cultures, treated without or with 1 nm to 1 microm RAD001, 10 nm cabergoline, 10 nm SOM230 (a somatostatin receptor multiligand), and/or 50 nm IGF-I. Cell viability and apoptosis were evaluated after 48 h, and vascular endothelial growth factor (VEGF) secretion was assessed after an 8-h incubation. Somatostatin and dopamine subtype 2 receptor expression was investigated by quantitative PCR. RESULTS In 28 cultures (70%), Everolimus significantly reduced cell viability (by approximately 40%; P < 0.05 vs. control), promoted apoptosis (+30%; P < 0.05 vs. control), inhibited p70S6K activity (-20%), and blocked IGF-I proliferative and antiapoptotic effects. In selected tissues cotreatment with SOM230, but not cabergoline, exerted an additive effect. Everolimus did not affect VEGF secretion but blocked the stimulatory effects of IGF-I on this parameter. CONCLUSIONS Everolimus reduced NFA cell viability by inducing apoptosis, with a mechanism likely involving IGF-I signaling but not VEGF secretion, suggesting that it might represent a possible medical treatment of invasive/recurrent NFAs.
Immune responses in bovine tuberculosis.

Knowledge of the immune responses which develop in cattle following infection with Mycobacterium bovis is essential both to the understanding of disease pathogenesis and to the logical development of immune-dependent tools, such as diagnostic tests and vaccines, which can be used to combat the disease. Studies of field cases of bovine tuberculosis (TB) and of experimental bovine models of M. bovis infection have indicated that cell-mediated immune responses (CMI) predominate within a spectrum of immunity which exists. This paper reviews aspects of recent research and indicates how knowledge of T-cell antigenic targets in bovine TB along with increasing knowledge of T-cell subpopulations and their interactions with M. bovis -infected macrophages provides opportunities for the development of better methods for disease control.
Deconvolution of Seismic Data - An Overview

It is common practice to model a seismic trace as a convolution of the reflectivity function of the earth and an energy waveform referred to as the seismic wavelet. The objective of deconvolution is to extract the reflectivity function from the seismic trace. We will describe four techniques which have been and are being used to accomplish this objective. These techniques are predictive deconvolution, homomorphic filtering, Kalman filtering, and deterministic deconvolution. In addition, we shall outline the physical effects governing the bandwidth of the seismic data such as shooting geometry, recording filters, and absorption.
Short-term change in physical function and disability: the Women's Health and Aging Study.

OBJECTIVES Although measures of physical function are predictive of future disability, little is known about the short-term impact of changes in physical function on disability. METHODS Data from 93 of the 102 women who participated in the Weekly Substudy of the Women's Health and Aging Study (WHAS) were used to explore the association of changes in physical function with disability. The WHAS Substudy included 24 weekly assessments of three standard performance tests and self-reported disability in activities of daily living (ADLs) and basic mobility. RESULTS Using random-effects models, we found small but significant (ps <.01) changes in ADL and mobility disability during weekly follow-up. Baseline performance scores were significantly associated with both ADL and mobility disability (ps <.001), accounting for 27% and 36% of the between-person variability in each type of disability, respectively. After adjustment for baseline scores, change in performance scores was significantly associated with ADL disability (beta = 0.08, p <.01) and mobility disability (beta = 0.12, p <.001), but accounted only for a small proportion (<10%) of the variability in the rate of change in disability outcomes. There was no evidence for an additional effect on either type of disability because of having a single episode of a higher or lower than usual performance score, or because of periods of at least 4 consecutive higher or lower than usual performance test scores. DISCUSSION Basic physical functions account for a substantial proportion of the heterogeneity in ADL and mobility disability among older disabled women, but have a relatively small impact on short-term changes in either type of disability. Effective prevention of disability may require attention to a wider array of risk factors than just limitations in basic physical functions.
Eating Behavior in Bulimia a

Despite our strong belief in the utility of laboratory studies of eating behavior, we also note several caveats on the data thereby obtained. First, it must be assumed that subjects' behavior is influenced by the laboratory environment and is not identical to eating behavior in a "normal" setting. Second, not all bulimic subjects who were screened for these studies actually participated, so that it is possible that the sample of patients from whom we obtained data differed in some ways from a general clinical population of women with bulimia. Nonetheless, we believe that our data provide compelling evidence that the disturbed eating behavior characteristic of bulimia nervosa can be profitably studied in the laboratory. Even under structured laboratory conditions, most bulimic patients rated one of their multicourse meals as typical of a binge, and, during that meal, consumed a much larger amount of food and ate more rapidly than did controls who were asked to overeat. The significant correlations between the sizes of the multicourse and single-course binge meals and between the size of laboratory binge meals and the size of the "naturally occurring" binge meals reported to the dietician suggest that a reproducible phenomenon is being examined. The results of our studies suggest that the abnormalities of eating behavior in bulimia nervosa cannot be viewed simply as a disturbance of carbohydrate consumption or even as the episodic consumption of a certain type of food. Rather, eating behavior in this syndrome appears more generally disturbed. The most striking difference between the binge and the nonbinge meals of bulimic patients and between the binge eating of patients and the overeating of normal persons is the amount of food consumed, not the macronutrient composition of the meals. In addition, for all four meal types, the patients were hungrier after the end of the meal than were the controls, even though the patients' average caloric intakes were generally larger and their average hunger ratings before the meals did not differ from those of the controls. Certainly, self-induced vomiting may contribute to this abnormality, but it was also observed after nonbinge meals when vomiting did not occur. Together, these data are consistent with the notion that the essential appetitive abnormality in bulimia nervosa lies in the control of the amount of food consumed, not in the consumption of a particular macronutrient or type of food. Patients with bulimia nervosa appear less responsive than normal to the signals that lead to the termination of a meal. (ABSTRACT TRUNCATED AT 400 WORDS)
Percentage of gestational diabetes mellitus attributable to overweight and obesity.

OBJECTIVES We calculated the percentage of gestational diabetes mellitus (GDM) attributable to overweight and obesity. METHODS We analyzed 2004 through 2006 data from 7 states using the Pregnancy Risk Assessment Monitoring System linked to revised 2003 birth certificate information. We used logistic regression to estimate the magnitude of the association between prepregnancy body mass index (BMI) and GDM and calculated the percentage of GDM attributable to overweight and obesity. RESULTS GDM prevalence rates by BMI category were as follows: underweight (13-18.4 kg/m(2)), 0.7%; normal weight (18.5-24.9 kg/m(2)), 2.3%; overweight (25-29.9 kg/m(2)), 4.8%; obese (30-34.9 kg/m(2)), 5.5%; and extremely obese (35-64.9 kg/m(2)), 11.5%. Percentages of GDM attributable to overweight, obesity, and extreme obesity were 15.4% (95% confidence interval [CI] = 8.6, 22.2), 9.7% (95% CI = 5.2, 14.3), and 21.1% (CI = 15.2, 26.9), respectively. The overall population-attributable fraction was 46.2% (95% CI = 36.1, 56.3). CONCLUSIONS If all overweight and obese women (BMI of 25 kg/m(2) or above) had a GDM risk equal to that of normal-weight women, nearly half of GDM cases could be prevented. Public health efforts to reduce prepregnancy BMI by promoting physical activity and healthy eating among women of reproductive age should be intensified.
Intermittent microclimate cooling during exercise-heat stress in US army chemical protective clothing

The effectiveness of intermittent, microclimate cooling for men who worked in US Army chemical protective clothing (modified mission-oriented protective posture level 3; MOPP 3) was examined. The hypothesis was that intermittent cooling on a 2 min on–off schedule using a liquid cooling garment (LCG) covering 72% of the body surface area would reduce heat strain comparably to constant cooling. Four male subjects completed three experiments at 30°C, 30% relative humidity wearing the LCG under the MOPP 3 during 80 min of treadmill walking at 224 ± 5 W · m−2. Water temperature to the LCG was held constant at 21°C. The experiments were; 1) constant cooling (CC); 2) intermittent cooling at 2-min intervals (IC); 3) no cooling (NC). Core temperature increased (1.6 ± 0.2°C) in NC, which was greater than IC (0.5 ± 0.2°C) and CC (0.5 ± 0.3°C) ( p < 0.05). Mean skin temperature was higher during NC (36.1 ± 0.4°C) than IC (33.7 ± 0.6°C) and CC (32.6 ± 0.6°C) and mean skin temperature was higher during IC than CC ( p < 0.05). Mean heart rate during NC (139 ± 9 b · min−1) was greater than IC (110 ± 10 b · min−1) and CC (107 ± 9 b · min−1) ( p < 0.05). Cooling by conduction (K) during NC (94 ± 4 W · m−2) was lower than IC (142 ± 7 W · m−2) and CC (146 ± 4 W · m−2) ( p < 0.05). These findings suggest that IC provided a favourable skin to LCG gradient for heat dissipation by conduction and reduced heat strain comparable to CC during exercise-heat stress in chemical protective clothing.
Suppressing Zero-Sequence Circulating Current of Modular Interleaved Three-Phase Converters Using Carrier Phase Shift PWM

Zero-sequence circulating current (ZSCC) has been a major concern for the operation of paralleled converters. Existing ZSCC reduction methods in the literature either suffer poor performances or are too complicated for modular implementation in an arbitrary number of interleaved converters. This paper investigates the application of interleaved carrier phase-shift (ICPS) pulse-width modulation (PWM) in modular interleaved converter system for ZSCC peak value reduction. The generalized study based on the derived ZSCC analytical expression reveals that the ICPS PWM can achieve superior ZSCC peak value reduction performance regardless the number of interleaved converters. Moreover, the impacts of ICPS PWM on the output voltage and current quality are also analyzed based on double Fourier integral. Compared with the conventional interleaved sinusoidal PWM (ISPWM), the output quality with the ICPS PWM can be comparable or even better. Simulation and experimental results show good agreement with the theoretical analysis, verifying the performance of the ICPS PWM.
New measurement method to characterize piezoelectric saw substrates at very high temperature

In this paper we present a new experimental set-up leading to characterize SAW sensor properties in high temperature up to 900degC. The characterization method consists in hanging a small piece of self-warming piezoelectric SAW device in a vacuum chamber. The device is made of the piezoelectric material to be tested equipped with its IDT plus a heating resistance, both in Platinum. The whole system is suspended from a PCB by mean of classical bonding wires. It is therefore thermally isolated from the rest of the experimental set-up. This allows using standard low-cost circuitry, to connect the SAW device to the measurement apparatus (standard coaxial feed-lines and SMA connectors). The warming being localised on the piezoelectric substrate, it also becomes possible to reach very high temperature, quickly and at low energy cost. This allows easy making of temperature cycles to test the aging of materials. In a first step, TCF values for Quartz ST and LiNbO3 Y-128deg were measured in the range [20-500degC], then compared to calculated ones in order to validate the method. In a second step, one LGS Y-X SAW Delay-Line with Pt/Ta IDT was characterized using this test method in the range [20-900degC].
Cooperative Control of Underwater Glider Fleets by Fault Tolerant Decentralized MPC

Abstract A fleet of Autonomous Underwater Vehicles (AUVs) moving together in a prescribed pattern can form an efficient data acquisition network. The problem is to control several AUVs such that after transients, they form a required formation and move along a desired trajectory. The capability to accomplish the mission even in case of faults, is a fundamental requirement for these kind of missions. A completely decentralized predictive control and FDI strategy is here proposed for allowing cooperation. Trough an underwater communication channel, each vehicle broadcasts its position, its future behavior and its actuator/sensor fault situation. Based both on the local and the received information, each vehicle selects the desired formation to keep and plans its future actions. Simulation results are provided to validate the approach.
Development of Isotopic and Non‐Isotopic Microwell Based Immunoassays for hCG Using 125I and Biotin Labeled hCG

Abstract Isotopic and non‐isotopic immunoassays of hCG, based on the principle of competitive inhibition, using micro‐well as solid support and 125I and biotin as labels for hCG, have been developed. In both the assays, rabbit polyclonal antibody was immobilized onto micro‐wells. In the non‐isotopic assay, to the hCG antibody coated micro‐wells, 50 µL of standard or samples along with 100 µL of biotinylated hCG were incubated for 1 hour at 37°C. After incubation, wells were washed and 100 µL of streptavidin‐HRP conjugate was added to each well and incubated again for a half hour at 37°C. Bound enzyme activity was measured using tertramethyl benzidine/hydrogen peroxide (TMB/H2O2) as substrate. In the isotopic assay, to the hCG antibody coated micro‐wells, 50 µL of standard or samples along with 100 µL of 125I‐hCG were incubated for 1 hour at 37°C. The bound radioactivity was measured using a gamma counter. The sensitivities of the non‐isotopic and isotopic assays were 0.12 IU/mL and 0.1 IU/mL, respectively. The intra‐ and inter‐assay CVs for both the assays were less than 12.3%. There was a good correlation between the developed non‐isotopic and isotopic immunoassays (r=0.97, n=20).
A Rice HAL2-like Gene Encodes a Ca2+-sensitive 3′(2′),5′-Diphosphonucleoside 3′(2′)-Phosphohydrolase and Complements Yeast met22 and Escherichia coli cysQ Mutations (*)

A plant homolog of yeast HAL2 gene (RHL) was cloned from rice (Orizya sativa L.). The RHL cDNA complemented an Escherichia coli cysteine auxotrophic mutant, cysQ, and the yeast HAL2 mutant, met22. The latter is a methionine auxotroph and cannot use sulfate, sulfite, or sulfide as sulfur sources but exhibits wild-type activities of the enzymes necessary to assimilate sulfate and has normal sulfur uptake system. These results demonstrated that HAL2, cysQ, and RHL genes encode proteins with similar function in sulfur assimilatory pathway. The RHL cDNA expressed a 40-kDa protein that was shown to catalyze the conversion of adenosine 3′-phosphate 5′-phosphosulfate (PAPS) to adenosine 5′-phosphosulfate (APS) and 3′(2′)-phosphoadenosine 5′-phosphate (PAP) to AMP. The enzyme activity is Mg2+-dependent, sensitive to Ca2+, Li+, and Na+ and activated by K+. The inhibition by Ca2+ depends on the Mg2+/Ca2+ ratio and is reversible by high Mg2+ concentration. The substrate specificity and kinetics of RHL enzyme are very similar to the Chlorella 3′(2′),5′-diphosphonucleoside 3′(2′)-phosphohydrolase (DPNPase). Our evidence suggests that this enzyme regulates the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. Several residues that are essential for the activity of this enzyme were identified by site-directed mutagenesis, and the possible role of DPNPase in salt tolerance is discussed.
Denoising of Rain Streaks Patterns in Images by Decomposition on Morphological Component Analysis: A Review

-Rain produces intensity fluctuations in images and videos. Rain streaks removing method works on video. There are very few methods which address the problem of ran streaks removal by image decomposition based on MCA solved by performing dictionary learning and sparse coding. Instead of applying traditional MCA method, the proposed method first decomposes an image into the low and high frequency parts using smoothing filter. The HF part is then decomposed into rain component and non-rain component by performing dictionary learning and sparse coding. The experimental results exposes the rain component can be successfully removed from an image. repared. In the end future access has been predicted for a particular webpage by a particular IP address. This approach can be used for that webpages where there are similar patterns of access. extraction technique is also been discussed.
Cell death in the endocardial cushions of the developing heart.

The incidence of apoptotic cells in the hearts of chick embryos between days 4 and 8 of development was examined using an in situ technique for the detection of DNA fragmentation. Using this method it was possible to demonstrate foci of apoptotic cells primarily in two locations: the outflow tract cushions and the atrioventricular cushions. Both occurred only during narrow time windows: between embryonic days 4.5 and 6.5 in the outflow tract, and between embryonic days 5.5 and 7.5 in the atrioventricular canal. This is a much more restricted distribution of dying cells than previously thought, with reproducible cell death notably absent from the atrial and ventricular walls. Dying cells were also unexpectedly absent from the fusion seam of apposed cushions. In a complementary study, cell proliferation in these tissues was examined over the same time period using the expression of proliferating cell nuclear antigen as a marker for dividing cells. Cell proliferation occurred throughout the region of the cushions at these stages, including the myocardium and the fusion points of the apposed cushions. It is concluded that cells undergoing programmed cell death at this time in the developing chick heart are abundant in, and largely restricted to, the cushion tissue, and that cushion morphogenesis is regulated by the co-ordination of cell transformation, cell proliferation and, during a narrow time window, cell death.
Three-point correlation functions in uniformly and randomly driven diffusive systems.

Driven far away from equilibrium by both uniform and random external fields, a system of diffusing particles with short-range attractive forces displays many singular thermodynamic properties. Surprisingly, measuring pair correlations in lattice-gas models with saturation drives, we find little difference between the uniform and random cases, even though the underlying symmetries are quite distinct. Motivated by this puzzle, we study three-point correlations using both field-theoretic and simulation techniques. The continuum theory predicts the following: (a) The three-point function is nonzero only for the uniformly driven system; (b) it is odd under a parity transformation; and (c) there exists an infinite discontinuity singularity at the origin in momentum space. Simulation results are clearly consistent with these predictions. Based on these findings, we suggest several avenues for future investigations.