Miracle-dz/UniFAR-FTUs huggingface dataset:
Cloning and Characterization of a Novel Sperm-Associated Isoantigen (E-3) with Defensin- and Lectin-Like Motifs Expressed in Rat Epididymis1

Abstract In the present study we report the identification of a novel epididymis-specific secretory glycoprotein, E-3, which is a sperm-associated isoantigen containing defensin- and lectin-like motifs. E-3 was detected in rat epididymal fluid and in sperm extracts by two-dimensional (2-D) Western blotting using rat hyperimmune sera raised against rat sperm. The immunoreactive spot of approximately 28 kDa with an isoelectric point (pI) of 3.5 was cored from silver-stained gels. Microsequencing by tandem mass spectrometry and database searches revealed several peptides to be novel sequences. Degenerate deoxyinosine-containing primers corresponding to the novel peptides were used in rapid amplification of cDNA ends and polymerase chain reaction to clone E-3 from a rat epididymal cDNA library. A 449-base pair nucleotide sequence was subsequently obtained consisting of a complete open reading frame (ORF) of 111 amino acids, which showed similarity to the defensin and lectin families. The first 21 amino acids constituted a putative signal peptide, suggesting that E-3 is a secretory protein. Mature E-3 protein corresponding to amino acids 22–111 was expressed in E. coli, and chickens were immunized with recombinant E-3 (rE-3). The resulting anti-rE-3 antisera recognized the recombinant immunogen as well as a “native” protein of 28 kDa, pI 2.5–3.5 in both epididymal fluid and in sperm extracts on 2-D Western blots. Northern hybridization indicated that E-3 mRNA was present in the epididymis but not in testis or other tissues, and that E-3 mRNA was predominantly expressed in the corpus and cauda of the epididymis, but not in the initial segment or caput. Similarly, Western blots detected the E-3 protein only in the epididymal fluid and sperm from the corpus and caudal regions. Finally, indirect immunofluorescence localized E-3 on the entire tail, and with less intensity on the head of the sperm. These observations indicate that E-3 is a secreted epididymal protein that becomes associated with the sperm as it transits through the corpus and cauda. The presence of a defensin-like motif suggests that E-3 may play a role in protecting the sperm from microbial infections in the epididymis and in the female reproductive tract.
The Impact of Drama Education on Creativity Development at Preschool Children

This paper points out at the importance of creativity development in children of preschool age and analyses certain conditions and pedagogical principles which should be respected during the development of creativity in kindergartens. Research survey focuses on the development of creativity reflection at children in kindergartens at preschool age and based on a test of creativity it compares creativity of children in experimental and control groups. The goal is to find out if there are any differences among children in experimental and control classrooms in kindergartens; wherein experimental groups, there is preschool education with the use of drama education while in control groups there is not. On the basis of certain aspects, the gained data is compared through descriptive methods and correlations. Research results refer to reserves in creativity development in modern pre-primary education in the context of implemented and expected changes in didactic approach in the education of kindergartens. Keywords—Preschool child, drama in education, research, test of creativity.
Design and Analysis of Pulse width Modulator (PWM) using Low Input Impedance Current Comparator

In this paper a new design of a simple pulse width modulator is presented. The proposed scheme has 3mW average power consumption which is less than the existing PWM methods. This circuit is also suitable for IC implementation. The simulation results are carried out under the implementation of TSMC 0. 35μm CMOS process with 1.8V power supply. This method is commonly used for driving motors, heaters, or lights in varying intensities or speeds. The simulation and experimental results are shown in this paper. It is also suitable for high power applications as well as ordinary low power use, while possessing important advantages. Pulse-width (PW) modulation has been widely used to convert analog (or multibit digital) signals to binary streams.
Trajectories of Callous-Unemotional Traits in Childhood Predict Different Forms of Peer Victimization in Adolescence

Callous-unemotional (CU) traits (e.g., lack of empathy and guilt) differentiate a group of children at particularly high risk for engaging in aggressive behavior, notably bullying. However, little is known about whether youths with CU traits are at risk for being victimized by their peers. We examined the associations between trajectories of CU traits in childhood (between 7 and 12 years old) and peer victimization in adolescence (14 years old). The participants were drawn from the Twins Early Development Study, a longitudinal population-based study of twins born in England and in Wales. The trajectories of CU traits (i.e., stable high, increasing, decreasing and stable low) were identified through general growth mixture modeling. Four forms of peer victimization were considered: physical victimization, verbal victimization, social manipulation, and attacks on property. We found that youths with stable high levels, increasing levels, and decreasing levels of CU traits in childhood had higher levels of physical victimization in adolescence, not explained by other predictors at age 7 (e.g., conduct problems). Youths with increasing levels of CU traits, compared with the ones with stable low levels, also had higher levels of verbal victimization, social manipulation, and attacks on property. Our findings highlight the importance of distinct trajectories of CU traits in accounting for the experience of different forms of peer victimization. Youths with CU traits may benefit from bullying prevention programs, as they are likely to be the targets of peer victimization.
University of

—This paper is focused on two major problems within the ceramics industry. The reproduction of a desired color from pigments (which is time-intensive), and the correction of colors on the production line (which is costly) are processes which still rely heavily on numerous experiments carried out by human operators. This study presents the key aspects of these two processes and suggests some mathematical and computer sciences tools, aimed at automatizing the current procedures. Data was provided by an industrial partner, where the proposed models and solutions will be tested and validated.
EAU Guidelines on testicular cancer : 2011 update 129 Table 1 Recommended pathologic classification

a Departamento de Urología, Universidad de Düsseldorf, Düsseldorf, Germany b Departamento de Urología, Rudolfstiftung, Viena, Austria c Departamento de Patología, Fundacio Puigvert, Barcelona, Spain d Departamento de Oncología Médica, Universitätskliniken Eppendorf, Hamburgo, Germany e Departamento de Oncología, Hospital Universitario Karolinska, Estocolmo, Sweden f Departamento de Medicina, Universidad de Paris XI, Villejuif, France g Unidad Académica de Radioterapia y Oncología, Royal Marsden NHS Trust & The Institute of Cancer Research, Sutton, United Kingdom h Departamento de Urología, Centro Médico de Amsterdam, Universidad de Amsterdam, Amsterdam, The Netherlands
Chloride dependence of frusemide‐ and phloretin‐sensitive passive sodium and potassium fluxes in human red cells.

1. In outdated human red blood cells, there was a Ko‐dependent, ouabain‐insensitive efflux of Na that was inhibited by frusemide and phloretin. 2. It was reduced or absent (a) in media containing Ca or Mg, (b) in fresh cells. 3. In media with Cl partly replaced by NO3, Na efflux, and its sensitivity to Ko and frusemide, were reduced. 4. The saturable component of ouabain‐insensitive K influx was dependent on Cl and inhibited by frusemide and by phloretin. 5. Bromide also supported a frusemide‐sensitive, ouabain‐insensitive K influx but acetate and SO4 (like NO3) did not.
Predictability and construct validity of oppositional defiant disorder in children and adolescents with ADHD combined type

Department of Child and Adolescent Psychiatry, University of Zurich, Switzerland MRC Social Genetic Developmental and Psychiatry Centre, Institute of Psychiatry, London, UK Department of Child and Adolescent Psychiatry and Psychotherapy, Central Institute of Mental Health, J 5, Mannheim, Germany Department of Psychiatry, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands Department of Psychology, Hebrew University, Jerusalem, Israel Department of Psychiatry, School of Medicine, Trinity College Dublin, Dublin, Ireland Geha MHC, Petach-Tikva, Israel Department of Developmental and Educational Psychology, University of Valencia, Valencia, Spain Clinic for Child and Adolescent Psychiatry and Psychotherapy, University of Duisburg-Essen, Essen, Germany Department of Experimental Clinical and Health Psychology, Ghent University, Ghent, Belgium Department of Child and Adolescent Psychiatry, University of Göttingen, Göttingen, Germany Department of Clinical Neuropsychology, Vrije Universiteit, Amsterdam, The Netherlands School of Psychology, University of Southampton, Southampton, UK Departments of Psychiatry and of Neuroscience and Physiology, SUNY Upstate Medical University, Syracuse, NY, USA Aalborg Psychiatric Clinic, Aarhus University Hospital, Denmark Child and Adolescent Clinical Psychology, University of Basel, Switzerland
The Treatment of Open Tibial Shaft Fractures Using an Interlocked Intramedullary Nail Without Reaming

Summary: Between January 1989 and September 1991, 117 consecutive open tibial shaft fractures were treated at our institution. Of these, 64 (55%) met the inclusion criteria and were prospectively treated according to protocol using unreamed interlocking intramedullary nails as definitive fixation. Wounds were classified according to the method of Gustilo et al., and included 10 type I, 16 type II, and 38 type III (17 type IIIA, 21 type IIIB) wounds. Contraindications to intramedullary nailing included (a) fractures involving the proximal or distal one fifth of the tibia, (b) patients with open physes, and (c) an associated vascular injury (type IIIC). Proximal locking was routinely performed, whereas distal locking was used as needed for axial and/or rotational stability. Soft-tissue coverage was obtained after adequate debridement within 7 days: 26 of 64 fractures (41%) required a soft-tissue procedure (17 split-thickness skin grafts, eight free-tissue transfers, one rotational muscle flap). Patients were encouraged to bear full weight in a short leg cast or Sarmiento brace as soon as other injuries or pain permitted. Average follow-up time was 24.8 months (range 12–44) and was possible in 46 fractures (71.875%; nine of 10 type I, 12 of 16 type II, 10 of 17 type IIIA, and 15 of 21 type IIIB). Mean time to healing was as follows: type I, 4.8 months; type II, 4.7 months; type IIIA, 8.28 months; and type IIIB, 9.30 months. Twenty fractures exhibited a delay in healing (>6 months). This included two of 12 type II (16%), six of 10 type IIIA (60%), and 12 of 15 type IIIB fractures (80%). Nine received no treatment, five underwent exchange nailing with a reamed nail, and six were bone grafted. All but one went on to uneventful healing. There were no malunions in either rotation, angulation, or length. Clinically, patients complained of only occasional pain. All had full range of motion of the knee and ankle unless associated with other pathology. Complications included one bent nail and 12 broken screws in 10 fractures (15%). There were six acute infections (13%), all in type III fractures (one IIIA, five IIIB). Two of these patients developed chronic osteomyelitis requiring saucerization; both had type IIIB fractures. Our data indicate that unreamed tibial nailing is an acceptable technique for use in all open tibial shaft fractures (excluding type IIIC). Our overall chronic infection rate was 4%, with no chronic infections in types I, II, and IIIA open fractures and a 13% rate in type IIIB open fractures. In addition, this series was not associated with any malunions. Although delayed union was prevalent, it appeared to be related to the amount of soft-tissue stripping and nail stiffness. The fact that all but one fracture healed with excellent alignment and a minimal infection rate makes this technique suitable for the stabilization of open tibial shaft fractures.
Breakdown in a high power microwave antenna

In this work, a high power microwave (HPM) antenna is designed and its breakdown limits are investigated. It has been shown that the designed antenna can handle Giga Watt (GW) power without attaining breakdown. The flash-over which usually occurs at the interface of HPM antennas, where microwave leaves and enters into the air, was controlled by adjusting the field intensity at the output window of the antenna. It has been shown that by adopting Gaussian beam distribution at the aperture of horn with appropriate beam waist, the E-field intensity at the output window can be reduced significantly. It has also been demonstrated that an HPM antenna with 0.4 m aperture radius can sustain >30 GW power for a pulse duration of 1ns and the same is reduced to <;5 GW for the pulse having duration > 1μs.
Targeted disruption of Sonic Hedgehog in the mouse adrenal leads to adrenocortical hypoplasia

Development of the mammalian adrenal gland is regulated by a diverse network of growth and transcription factors. Disruptions in these pathways often result in adrenal insufficiency because of adrenal hypoplasia. Several lines of evidence have suggested that the Hedgehog signaling pathway, which controls many aspects of tissue and organ patterning, may play a direct role in adrenal morphogenesis as well. Therefore, we examined the role of Sonic Hedgehog (Shh), a member of the Hedgehog family, in mouse adrenal development. We show that Shh and its downstream effectors Gli1, Gli2, and Gli3 are expressed in the adrenal cortex throughout development, and that Shh is required for normal adrenal organogenesis. Conditional inactivation of Shh in the adrenal cortex using a Cre‐loxP system resulted in severe hypoplasia and disorganization of the cortex. In mice carrying the targeted mutation (Shhfl/fl;SF‐1/Cre+), adrenal mass was significantly reduced and the cortex failed to encapsulate the adrenal medulla. Taken together, these results establish a direct role for Shh signaling in normal adrenal development. genesis 47:628–637, 2009. © 2009 Wiley‐Liss, Inc.
A RESTful web networking framework for vital sign monitoring

The burgeoning cost of healthcare has forced industry experts and academics to rethink current healthcare systems. There is a compelling argument that the increasing costs are primarily due to the fundamentally reactionary approach used in healthcare today, with the focus being on treatment and cure rather than on prevention. Consequently, building a sustainable healthcare system requires a paradigm shift towards a proactive prevention based approach. A critical requirement of such a system is the ubiquitous collection of and access to patient physiological data. Both real-time and historical analysis of this data is the basis of a preventive healthcare system. To this end, we are developing a pervasive real-time health monitoring framework to seamlessly connect both patients and healthy people to healthcare professionals. In this paper, we present the design of this framework, which is intended to be lightweight, agile and scalable. The design primarily utilizes a resource-oriented architecture (ROA) based RESTful HTTP to connect wireless biosensors, wireless networks and a cloud computing platform. The paper also discusses a proof-of-concept implementation of the framework that demonstrates the effectiveness of the technology choices made in achieving the required design goals.
DIAGNOSTIC VIROLOGY PRACTICES FOR RESPIRATORY SYNCYTIAL VIRUS AND INFLUENZA VIRUS AMONG CHILDREN IN THE HOSPITAL SETTING: A NATIONAL SURVEY

A survey was sent to the emergency room and laboratory directors of 400 randomly selected US hospitals to assess the diagnostic testing practices for respiratory syncytial virus and influenza virus in children. The results demonstrate that the majority of hospitals routinely perform viral testing for both viruses and use virology testing practices appropriate for the reasons reported for testing.
Synchronization of a group of mobile agents with supercritical connectivity radius and large population

This paper investigates the synchronization behavior of a class of mobile agents based on the Vicsek's model. It is well-known that the connectivity of the dynamical neighbor graphs is important for synchronization of the model, but such a connectivity is defined by the system trajectories, which, in turn, are determined by the model parameters and the initial configuration of all agents. We will carry out our analysis under the assumptions that all agents are independently and uniformly distributed in [0, 1]2 at the initial time, and that the interaction radius is taken as the supercritical connectivity radius O(√log n/n). By estimating the spectral gap of the average matrix of the initial neighbor graph with the supercritical connectivity radius, we will establish the synchronization condition, which is imposed on the moving speed of the agents only.
The Reliability of the Modified Balance Error Scoring System

Objective:Study 1 investigated the intraclass reliability and percent variance associated with each component within the traditional Balance Error Scoring System (BESS) protocol. Study 2 investigated the reliability of subsequent modifications of the BESS. Design:Prospective cross-sectional examination of the traditional and modified BESS protocols. Setting:Schools participating in Georgia High School Athletics Association. Intervention:The modified BESS consisted of 2 surfaces (firm and foam) and 2 stances (single-leg and tandem-leg stance) repeated for a total of three 20-second trials. Participants:Participants consisted of 2 independent samples of high school athletes aged 13 to 19 years. Main Outcome Measures:Percent variance for each condition of the BESS was obtained using GENOVA 3.1. An intraclass reliability coefficient and repeated measures analysis of variance were calculated using SPSS 13.0. Results:Study 1 obtained an intraclass correlation coefficient (r = 0.60) with stance accounting for 55% of the total variance. Removing the double-leg stance increased the intraclass correlation coefficient (r = 0.71). Study 2 found a statistically significant difference between trials 1 and 2 (F(1.65,286) = 4.890, P = 0.013) and intraclass reliability coefficient of r = 0.88 for 3 trials of 4 conditions. Conclusions:The variance associated with the double-leg stance was very small, and when removed, the intraclass reliability coefficient of the BESS increased. Removal of the double-leg stance and addition of 3 trials of 4 conditions provided an easily administered, cost-effective, time-efficient tool that provides reliable objective information for clinicians to base clinical decisions upon.
Reliability of on-call radiology residents’ interpretation of 64-slice CT pulmonary angiography for the detection of pulmonary embolism: methodological error

We were interested to read the paper by Joshi and colleagues (1) published online in Acta Radiologica in October 2013. In many teaching hospitals, radiology residents are the first to review a case and to make an initial interpretation of the images – therefore accurate diagnosis of pulmonary embolism (PE) is crucial, especially in an emergency care setting. The authors aimed to evaluate the discrepancies (inter-observer reliability) between resident and staff interpretations of 64-slice computed tomography (CT) angiogram for PE. It is crucial to mention that reliability (precision) and validity (accuracy) are two completely different methodological issues which are being assessed by different statistical tests and should not be confused with each other (2–4). As the authors point out in their results, discrepancies were noted in 25 of the 215 studies (11.6%) (1). Why did the authors report their results descriptively and not use well-known statistical tests for reliability analysis (2–5)? They concluded that it is reasonable to have on-call radiology residents perform the preliminary interpretations of 64-slice CT for PE studies. Such a conclusion is a mistake due to misinterpretation of the results and also the inappropriate use of statistical tests for reliability and validity analysis, and should really be avoided by researchers (2–5). Briefly, for quantitative variable intraclass correlation coefficient (ICC) agreement and for qualitative variables, weighted kappa should be used with caution because kappa also has its own limitation in reliability analysis (2–5). Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio, and negative likelihood ratio as well as odds ratio (true/false results – preferably >50) are among the tests to evaluate the validity (accuracy) of a test compared to a gold standard (2–5). As a take home message, for reliability and validity analysis, appropriate tests should be applied by clinical researchers. Otherwise, misdiagnosis and mismanagement of the patients in routine clinical care cannot be avoided.
CORRELATION BETWEEN POROSITY OF POROUS SILICON AND OPTOELECTRONIC PROPERTIES

We calculate the optical constants and absorption coefficient of n-dopped porous silicon (PS), by using Effective media approximation (EMA) analysis. Refractive index,n, decreases with increasing porosity in visible region. However, absorption coefficient, α, increases with increasing porosity in this region. Also energy band gap of PS has been determined using absorption spectra. Our results show that band gap of PS is quasidirect.
Design and Implementation of Middleware Based on ID and IP Address for Actuator Networks

Recently, the intelligent services demands interactions with the sensor networks, actuator networks, and context aware applications. Actuator network supports an infrastructure to have an immediate access to information for controlling the physical world and its objects using sensor networks and context aware applications. Actuator network middleware supports the command message transfer for controlling the behavior of the environment or physical systems. In this paper, we design and implement the actuator network middleware based on ID(IDentification) and IP(Internet Protocol) address for control the environment using multiple actuator devices. Also, this middleware assigns ID for identifying each actuator nodes, and uses an IP address for transferring control messages between application and actuator networks. And, the presented middleware provides to connect between actuator ID and IP address using the mapping table. We design the middleware using sequence diagram and state diagram, and implement it based on . Net framework. The middleware supports to transfer command messages between application and actuator networks.