Miracle-dz/UniFAR-FTUs huggingface dataset:
Pollen immunotherapy reduces the development of asthma in children with seasonal rhinoconjunctivitis (the PAT-study).

BACKGROUND Children with allergic rhinitis are likely to develop asthma. OBJECTIVE The purpose of this investigation was to determine whether specific immunotherapy can prevent the development of asthma and reduce bronchial hyperresponsiveness in children with seasonal allergic rhinoconjunctivitis. METHODS From 6 pediatric allergy centers, 205 children aged 6 to 14 years (mean age, 10.7 years) with grass and/or birch pollen allergy but without any other clinically important allergy were randomized either to receive specific immunotherapy for 3 years or to an open control group. All subjects had moderate to severe hay fever symptoms, but at inclusion none reported asthma with need of daily treatment. Symptomatic treatment was limited to loratadine, levocabastine, sodium cromoglycate, and nasal budesonide. Asthma was evaluated clinically and by peak flow. Methacholine bronchial provocation tests were carried out during the season(s) and during the winter. RESULTS Before the start of immunotherapy, 20% of the children had mild asthma symptoms during the pollen season(s). Among those without asthma, the actively treated children had significantly fewer asthma symptoms after 3 years as evaluated by clinical diagnosis (odds ratio, 2.52; P <.05). Methacholine bronchial provocation test results improved significant in the active group (P <.05). CONCLUSION Immunotherapy can reduce the development of asthma in children with seasonal rhinoconjunctivitis.
Clinical and MRI findings associated with false-positive knee MR diagnoses of medial meniscal tears.

OBJECTIVE The objective of our study was to determine if false-positive MR diagnoses of a medial meniscal tear are more common with specific clinical variables, tear type or location, or MRI findings of a longitudinal tear. MATERIALS AND METHODS We reviewed the records of 559 patients who underwent knee MR examinations and arthroscopy. We compared the positive predictive values (PPVs) of an MR diagnosis of a medial meniscal tear for differences in tear location or type, delay between knee injury and MRI, delay between MRI and arthroscopy, and the presence of an anterior cruciate ligament (ACL) tear. We also retrospectively reviewed the MR examinations of 50 longitudinal tears to compare the PPVs of various MRI findings of a longitudinal tear. RESULTS There was no association between either the delay between injury and MRI or the delay between MRI and arthroscopy and false-positive diagnoses. The PPV of 64% (32/50) for longitudinal tears was lower than the values of 83% (15/18) to 100% (116/116) for other types of medial meniscal tears. False-positive diagnoses of medial meniscal tears were more common in patients who had a prior episode of acute trauma (p = 0.004) or an ACL tear (p < 0.0001). Review of longitudinal tears revealed a decreased PPV when MRI showed signal contacting only the superior surface (p = 0.016) or when MRI showed signal contacting the surface at the meniscocapsular junction (p = 0.004). Four of the 18 menisci with a false-positive diagnosis of a longitudinal tear had a healed ACL tear noted at arthroscopy. CONCLUSION False-positive MR diagnoses of medial meniscal tears are more common for longitudinal tears than other tear types and are also more common with MR abnormalities at either the superior surface or the meniscocapsular junction. Spontaneous healing of longitudinal tears accounts for some false-positive MR diagnoses.
Social Inclusion, Poverty Eradication and the 2030 Agenda for Sustainable Development

The current paper analyses the concept of social inclusion at the global level and examines various policies at the national level that have been implemented to achieve inclusive and sustainable outcomes. It argues that there are three key interrelated areas that are critical for poverty eradication and inclusive development, which include (i) universal social protection; (ii) meaningful participation; and (iii) social and solidarity economy. It concludes that development is unlikely to be sustainable unless it is inclusive. The 2030 Agenda for Sustainable Development thus needs to be an inclusive plan of action, pursuing the goal of leaving no one behind in a way that meets the needs of present generations without compromising the ability of future generations to participate in the processes that impact their lives.
Homozygously deleted gene DACH1 regulates tumor-initiating activity of glioma cells

Loss or reduction in function of tumor suppressor genes contributes to tumorigenesis. Here, by allelic DNA copy number analysis using single-nucleotide polymorphism genotyping array and mass spectrometry, we report homozygous deletion in glioblastoma multiformes at chromosome 13q21, where DACH1 gene is located. We found decreased cell proliferation of a series of glioma cell lines by forced expression of DACH1. We then generated U87TR-Da glioma cells, where DACH1 expression could be activated by exposure of the cells to doxycycline. Both ex vivo cellular proliferation and in vivo growth of s.c. transplanted tumors in mice are reduced in U87TR-Da cells with DACH1 expression (U87-DACH1-high), compared with DACH1-nonexpressing U87TR-Da cells (U87-DACH1-low). U87-DACH1-low cells form spheroids with CD133 and Nestin expression in serum-free medium but U87-DACH1-high cells do not. Compared with spheroid-forming U87-DACH1-low cells, adherent U87-DACH1-high cells display lower tumorigenicity, indicating DACH1 decreases the number of tumor-initiating cells. Gene expression analysis and chromatin immunoprecipitation assay reveal that fibroblast growth factor 2 (FGF2/bFGF) is transcriptionally repressed by DACH1, especially in cells cultured in serum-free medium. Exogenous bFGF rescues spheroid-forming activity and tumorigenicity of the U87-DACH1-high cells, suggesting that loss of DACH1 increases the number of tumor-initiating cells through transcriptional activation of bFGF. These results illustrate that DACH1 is a distinctive tumor suppressor, which does not only suppress growth of tumor cells but also regulates bFGF-mediated tumor-initiating activity of glioma cells.
Assessment of idiopathic normal pressure patients in neurological practice: the role of lumbar infusion testing for referral of patients to neurosurgery

Background and purpose: In neurological practice patients with tentative idiopathic normal pressure hydrocephalus (iNPH) usually are referred to neurosurgery based on clinical and radiological findings. Hydrodynamic assessment using lumbar infusion testing might be helpful in selecting patients. To retrospectively analyse lumbar infusion tests done in neurological practice in iNPH patients to see how infusion test results relate to the clinical course and shunt response.
Modulation of Collagen Synthesis by Transforming Growth Factor‐β in Keloid and Hypertrophic Scar Fibroblasts

Keloid and hypertrophic scars are fibrous growths characterized by overabundant collagen deposition. We examined the effect of transforming growth factor-β (TGF-β), a known stimulant for the production of connective tissue matrices, on the rate of collagen synthesis in keloid fibroblasts (KFs), hypertrophic scar fibroblasts (HSFs), and normal skin fibroblasts (NSFs). Fibroblasts were cultured in three-dimensional fibrin-gel matrices in the presence or absence of TGF-β (5 ng/ml) or anti–TGF-β neutralizing antibody (50 μ/ml). Secreted collagen levels, labeled with 3H-proline, were measured after 48 hours. KFs produced up to 12 times more collagen than NSFs, and up to 4 times more than HSFs. Although KFs increased their rate of collagen production by up to 2.7 times in response to TGF-β, HSFs and NSFs did not (p = 0.065). Anti–TGF-β antibody reduced the rate of collagen synthesis of KFs by 40% (p = 0.003), although it did not suppress collagen production in HSFs (p = 0.06) and NSFs (p = 0.75). We conclude that although KFs and HSFs are similar in that they both overproduce collagen, they are different in that only KFs display a marked sensitivity to TGF-β, which is abundant during the proliferative phase of wound healing.
Optimizing Heat Acclimation for Endurance Athletes: High- Versus Low-Intensity Training.

PURPOSE To determine the effect of high- versus low-intensity training in the heat and ensuing taper period in the heat on endurance performance. METHODS In total, 19 well-trained triathletes undertook 5 days of normal training and a 1-wk taper including either low- (heat acclimation [HA-L], n = 10) or high-intensity (HA-H, n = 9) training sessions in the heat (30°C, 50% relative humidity). A control group (n = 10) reproduced their usual training in thermoneutral conditions. Indoor 20-km cycling time trials (35°C, 50% relative humidity) were performed before (Pre) and after the main heat exposure (Mid) and after the taper (Post). RESULTS Power output remained stable in the control group from Pre to Mid (effect size: -0.10 [0.26]) and increased from Mid to Post (0.18 [0.22]). The HA-L group demonstrated a progressive increase in performance from Pre to Mid (0.62 [0.33]) and from Mid to Post (0.53 [0.30]), alongside typical physiological signs of HA (reduced core temperature and heart rate and increased body-mass loss). While the HA-H group presented similar adaptations, increased perceived fatigue and decreased performance at Mid (-0.35 [0.26]) were evidenced and reversed at Post (0.50 [0.20]). No difference in power output was reported at Post between the HA-H and control groups. CONCLUSION HA-H can quickly induce functional overreaching in nonacclimatized endurance athletes. As it was associated with a weak subsequent performance supercompensation, coaches and athletes should pay particular attention to training monitoring during a final preparation in the heat and reduce training intensity when early signs of functional overreaching are identified.
Increasing UV-B Induces Biphasic Leaf Cell Expansion in Phaseolus vulgaris, Suggesting Multiple Mechanisms for Controlling Plant Growth

Abstract Leaf expansion, comprising cell division and cell enlargement, is controlled by light quality and quantity. The role of UV-B irradiance on leaf cell enlargement has not been determined. We studied the effect of a wide range of UV-B irradiances on the cell-enlargement-driven expansion of Phaseolus vulgaris L. , cv. Contender (bush bean) leaf discs. Our growth method allowed separation of the cell enlargement phase of leaf expansion from the cell division phase. In two series of experiments with different types of UV-B screening filters, the effect of increasing levels of UV-B on the area of excised P. vulgaris leaf discs was investigated. One set of experiments utilized polyester (UV-B-absorbing) and cellulose acetate (UV-B-transmitting) filters. The other set utilized UV-B-absorbing and UV-B-transmitting acrylic filters. Regardless of which type of filter was used for screening, high (above summer solstice) levels of supplemental UV-B inhibited cell enlargement in a linear, dose-dependent manner, resulting in smaller leaf discs than treatment with UV-B-absorbing filters. Conversely, low levels of supplemental UV-B enhanced cell enlargement in a linear, dose-dependent manner, resulting in larger leaf discs than did treatment with UV-B-absorbing filters. The results suggest a biphasic response to UV-B, and that there is an optimum UV-B level that results in maximum leaf expansion by cell enlargement.
Automating human intuition for protein design

In the design of new enzymes and binding proteins, human intuition is often used to modify computationally designed amino acid sequences prior to experimental characterization. The manual sequence changes involve both reversions of amino acid mutations back to the identity present in the parent scaffold and the introduction of residues making additional interactions with the binding partner or backing up first shell interactions. Automation of this manual sequence refinement process would allow more systematic evaluation and considerably reduce the amount of human designer effort involved. Here we introduce a benchmark for evaluating the ability of automated methods to recapitulate the sequence changes made to computer‐generated models by human designers, and use it to assess alternative computational methods. We find the best performance for a greedy one‐position‐at‐a‐time optimization protocol that utilizes metrics (such as shape complementarity) and local refinement methods too computationally expensive for global Monte Carlo (MC) sequence optimization. This protocol should be broadly useful for improving the stability and function of designed binding proteins. Proteins 2014; 82:858–866. © 2013 Wiley Periodicals, Inc.
Contraceptive vaccines targeting sperm

Overpopulation is a global problem of significant magnitude, with grave implications for the future. Development of new contraceptives is necessary, as existing forms of birth control are unavailable, impractical and/or too expensive for many individuals due to sociological, financial or educational limitations. Immunocontraception and, in particular, the targeting of an-tibodies to sperm-specific antigens implicated in sperm–egg binding and fertilisation offers an attractive approach to control fertility. Sperm-specific antibodies may impair fertility by inhibiting sperm motility, by reducing pe-netration of the cervical mucus by sperm, or by interfering in sperm capacitation or the acrosome reaction; alternatively, antisperm antibodies may invoke the complement cascade, resulting in sperm lysis. The antibodies raised against sperm-specific antigens have proved to be extremely effective at reducing sperm–egg interactions invitro; fertility trials in subhuman primates will eventually be needed to prove the effectiveness of the sperm antigens in terms of contraceptive efficacy before trials in humans can be justified. In addition, existing and emerging strategies (such as sperm proteomics, the determination of molecular and structural details of sperm proteins, and the modelling of protein–ligand interactions using X-ray and/or NMR structures to name a few) are expected to provide the experimental foundation for the design of small molecule inhibitors with antifertility effects. The technology underpinning vaccine development is constantly being developed and the introduction of DNA/RNA vaccines is certain to impact upon the field of immunocontraception.
Effect of Photonic Band Gap structure on Planar Antenna configuration

This paper describes the effect of Photonic Band Gap (PBG) structure as ground plane coupled with defected ground plane on Planar Antenna using coplanar waveguide with improvement of bandwidth and return loss value of 50% (in case of array of 3 × 5 UC-PBG) and 6.45 dB at resonant frequency of 3.6 GHz and is used for WIMax band (3.3 GHz to 3.8GHz) for Wireless Local Area Network (WLAN) applications.
MICRO-OPTO-FLUIDIC-SYSTEM ( MOFS ) FOR LABEL-FREE DETECTION OF WATERBORNE PATHOGENS IN DRINKING WATER

This paper reports a micro-opto-fluidic-system (MOFS) for real-time, high throughput and label-free detection of single particles in drinking water. The proposed system is based on a high flow rate achievable microfluidic chip and on chip multiple angle scattering (MALS) detection, which can detect the size and refractive index of single particle at flow rate as high as 10L/h. In the demonstrated experiment, polystyrene microspheres with different sizes under different solutions are detected and the size and RI measurements have precision of 0.1 μm and 0.005 respectively. Besides, real waterborne pathogens and particles such as Cryptosporidium parvum occysts, Giardia lamblia cysts, Escherichia Coli, sands and silts in drinking water are also detected by the system. The scattering patterns of different samples are distinctive and these results suggest that the waterborne pathogens can be easily detected and identified by the MOFS.
Identification of Target Genes of the p16INK4A-pRB-E2F Pathway*

Deregulation of the retinoblastoma protein (pRB) pathway is a hallmark of human cancer. The core members of this pathway include the tumor suppressor protein, pRB, which through binding to a number of cellular proteins, most notably members of the E2F transcription factor family, regulates progression through the cell division cycle. With the aim of identifying transcriptional changes provoked by deregulation of the pRB pathway, we have used cell lines that conditionally express a constitutively active phosphorylation site mutant of pRB (pRBΔCDK) or p16INK4A (p16). The expression of pRBΔCDK and p16 resulted in significant repression and activation of a large number of genes as measured by high density oligonucleotide array analysis. Transcriptional changes were found in genes that are essential for DNA replication and cell proliferation. In agreement with previous results, we found a high degree of overlap between genes regulated by p16 and pRB. Data we have obtained previously for E2F family members showed that 74 of the genes repressed by pRB and p16 were induced by the E2Fs and 23 genes that were induced by pRB and p16 were repressed by the E2Fs. Thus, we have identified 97 genes as physiological targets of the pRB pathway, and the further characterization of these genes should provide insights into how this pathway controls proliferation. We show that Gibbs sampling detects enrichment of several sequence motifs, including E2F consensus binding sites, in the upstream regions of these genes and use this enrichment in an in silico filtering process to refine microarray derived gene lists.
Robust dynamic risk prediction with longitudinal studies

ABSTRACT Providing accurate and dynamic age-specific risk prediction is a crucial step in precision medicine. In this manuscript, we introduce an approach for estimating the τ-year age-specific absolute risk directly via a flexible varying coefficient model. The approach facilitates the utilisation of predictors varying over an individual's lifetime. By using a nonparametric inverse probability weighted kernel estimating equation, the age-specific effects of risk factors are estimated without requiring the specification of the functional form. The approach allows borrowing information across individuals of similar ages, and therefore provides a practical solution for situations where the longitudinal information is only measured sparsely. We evaluate the performance of the proposed estimation and inference procedures with numerical studies, and make comparisons with existing methods in the literature. We illustrate the performance of our proposed approach by developing a dynamic prediction model using data from the Framingham Study.
Corporate Environmental Accounting: the issue, its practices and challenges: A study on Indian corporate accounting practices.

Environment is becoming a much more urgent economic, social and political problem all over the world. World is facing the twin problem of promoting economic development and protecting the environment. In this juncture the proper accounting of environmental effects on economic development is pre-requisite to have a sustainable development. It is observed that many efforts have been done to develop a mechanism of incorporating environmental data with national income to compute economic development. Moreover, all such attempts do not address the micro economic aspect of environmental data as such interaction between a specific firm and environment. The accounting of interaction between firm and environment is a pre-requisite to find out sustainability gap in micro level. This study attempts to address the development of corporate level environmental accounting and the problems associated with that. The study is under taken considering the environmental accounting and reporting adopted by sample companies in India . Key word: Environment, Accounting and reporting, environmental cost, liability and Assets
Epidural Hydromorphone for Postcesarean Analgesia

The efficacy of epidurally administered hydromorphone for postcesarean analgesia was evaluated in a prospective, randomized, double-blind study. Patients in group H (N = 26) received 1.0 mg of hydromorphone in preservative-free saline (total volume = 10 mL), administered epidurally. Patients in group B (N = 26) received 10 mL of 0.25% bupivacaine, administered epidurally. Both groups subsequently received intramuscular injections of hydromorphone as needed. There were significant differences between the two groups in pain score, patient assessment of analgesia quality, time to first analgesic intervention, and total dosage of hydromorphone during the first 24 hours. Nausea/vomiting and pruritis occurred more frequently in group H. No patient had a respiratory rate less than or equal to 10. There were no statistically significant differences between groups in mean times to first ambulation, first void, first passage of flatus, or hospital discharge.
Impulsivity: a discussion of clinical and experimental findings

Impulsivity can often be an important clinical problem in psychiatry and neurology. In psychiatry, the manifestation of impulsive behaviour in syndromes such as personality disorders, attention deficit hyperactivity disorder and in substance abuse may be different, and this has led to conflicting definitions. There has also been a tendency to concentrate on the nature of the behavioural manifestation (problems with the law, aggression, drug use, behavioural problems in school) rather than shared psychological processes, and to ignore the fact that impulsivity can also have positive aspects. In a normal population, the personality trait of impulsivity has been analysed using personality inventory questionnaires. Analysis of these data lead to the suggestion that impulsivity as commonly defined and understood may be made up of several independent factors, which may have separate biological bases. These self-rating questionnaires have been complemented by objective tests that are now often computerized, and which have been used in man (e.g. with criminal offenders, children, or patients who have undergone brain surgery). Some of these tests, such as the differential reinforcement of low rates procedure or the delay of reinforcement procedure, have also been used to study impulsivity in animals. Analysis of the behavioural principles of these tests suggests that they too may reflect different aspects of impulsivity. Many different biological systems have been proposed to contribute to the neurobiological basis of impulsivity. The serotonergic neurotransmitter system has recently received the most attention, with evidence of its involvement coming from animal studies as well as from studies in psychiatric patients. The frontal lobes have been proposed to play an important role in regulating impulsivity, although it unclear how specific this is. None of this biological knowledge has yet led to reliable pharmacotherapy for excessive impulsivity and, as yet, there is little understanding of the mechanisms by which those drugs, which have been found empirically to have some efficacy (e.g. the psychomotor stimulants in attention deficit hyperactivity disorder), exert their therapeutic effect. By bringing together knowledge from different areas of research it is hoped that a cross fertilization will be achieved, which will lead to a sharpening of concepts, an improvement in methodology and the stimulation of biological studies.