Miracle-dz/UniFAR-FTUs huggingface dataset:
Slc2a5 (Glut5) Is Essential for the Absorption of Fructose in the Intestine and Generation of Fructose-induced Hypertension*

The identity of the transporter responsible for fructose absorption in the intestine in vivo and its potential role in fructose-induced hypertension remain speculative. Here we demonstrate that Glut5 (Slc2a5) deletion reduced fructose absorption by ∼75% in the jejunum and decreased the concentration of serum fructose by ∼90% relative to wild-type mice on increased dietary fructose. When fed a control (60% starch) diet, Glut5-/- mice had normal blood pressure and displayed normal weight gain. However, whereas Glut5+/+ mice showed enhanced salt absorption in their jejuna in response to luminal fructose and developed systemic hypertension when fed a high fructose (60% fructose) diet for 14 weeks, Glut5-/- mice did not display fructose-stimulated salt absorption in their jejuna, and they experienced a significant impairment of nutrient absorption in their intestine with accompanying hypotension as early as 3–5 days after the start of a high fructose diet. Examination of the intestinal tract of Glut5-/- mice fed a high fructose diet revealed massive dilatation of the caecum and colon, consistent with severe malabsorption, along with a unique adaptive up-regulation of ion transporters. In contrast to the malabsorption of fructose, Glut5-/- mice did not exhibit an absorption defect when fed a high glucose (60% glucose) diet. We conclude that Glut5 is essential for the absorption of fructose in the intestine and plays a fundamental role in the generation of fructose-induced hypertension. Deletion of Glut5 results in a serious nutrient-absorptive defect and volume depletion only when the animals are fed a high fructose diet and is associated with compensatory adaptive up-regulation of ion-absorbing transporters in the colon.
Cognitive Heat

Current digital systems are largely blind to users’ cognitive states. Systems that adapt to users’ states show great potential for augmenting cognition and for creating novel user experiences. However, most approaches for sensing cognitive states, and cognitive load specifically, involve obtrusive technologies, such as physiological sensors attached to users’ bodies. This paper present an unobtrusive indicator of the users’ cognitive load based on thermal imaging that is applicable in real-world. We use a commercial thermal camera to monitor a person’s forehead and nose temperature changes to estimate their cognitive load. To assess the effect of different levels of cognitive load on facial temperature we conducted a user study with 12 participants. The study showed that different levels of the Stroop test and the complexity of reading texts affect facial temperature patterns, thereby giving a measure of cognitive load. To validate the feasibility for real-time assessments of cognitive load, we conducted a second study with 24 participants, we analyzed the temporal latency of temperature changes. Our system detected temperature changes with an average latency of 0.7 seconds after users were exposed to a stimulus, outperforming latency in related work that used other thermal imaging techniques. We provide empirical evidence showing how to unobtrusively detect changes in cognitive load in real-time. Our exploration of exposing users to different content types gives rise to thermal-based activity tracking, which facilitates new applications in the field of cognition-aware computing.
Mobilizing Institutions – Institutionalizing Movements – An Introduction

“Mobilizing institutions – institutionalizing movements”. Articles gathered for this volume of Focaal all question, from different angles and localities, the dichotomy between movements and institutions. The title reflects the very fluidity of the processes submitted to anthropological scrutiny. The articles all take their origins in what was to be originally two workshops, finally held jointly, for the EASA 6th Conference in Krakow in 2000 1. Starting from notions such as empowerment and popular participation, civil society, citizenship and the public sphere, both workshops aimed at exploring diverse representations of politics, collective forms of organization, and potentially new forms of relationships between the State, institutions and ‘civil society' or citizens. But while one was taking as its starting point social movements and participation, the other was set to explore institutional practices in the same field. At the fertile crossing of these two approaches lie the contributions gathered here.
PKA phosphorylates the p75 receptor and regulates its localization to lipid rafts

Although a large number of studies have been carried out on the diverse effects mediated by the common neurotrophin receptor p75NTR, little is known about the molecular mechanisms by which p75NTR initiates intracellular signal transduction. We identified a variant of the β catalytic subunit of cAMP‐dependent protein kinase (PKACβ) as a p75NTR‐interacting protein, which phosphorylates p75NTR at Ser304. Intracellular cAMP in cerebellar neurons was accumulated transiently by ligand binding to p75NTR. Activation of cAMP‐PKA is required for translocation of p75NTR to lipid rafts, and for biochemical and biological activities of p75NTR, such as inactivation of Rho and the neurite outgrowth. Proper recruitment of activated p75NTR to lipid rafts, structures that represent specialized signaling organelles, is of fundamental importance in determining p75NTR bioactivity.
Hedging Costs for Variable Annuities

A general methodology is described in which policyholder behaviour is decoupled from the pricing of a variable annuity based on the cost of hedging it, yielding two sequences of weakly coupled systems of partial differential equations (PDEs): the pricing and utility systems. The utility systems are used to generate policyholder withdrawal behaviour, which is in turn fed into the pricing systems as a means to determine the cost of hedging the contract. This approach allows us to incorporate the effects of utility-based pricing and factors such as taxation. As a case study, we consider the Guaranteed Lifelong Withdrawal and Death Benefits (GLWDB) contract. The pricing and utility systems for the GLWDB are derived under the assumption that the underlying asset follows a Markov regime-switching process. An implicit PDE method is used to solve both systems in tandem. We show that for a large class of utility functions, the two systems preserve homogeneity, allowing us to decrease the dimensionality of solutions. We also show that the associated control for the GLWDB is bang-bang, under which the work required to compute the optimal strategy is significantly reduced. We extend this result to provide the reader with sufficient conditions for a bang-bang control for a general variable annuity with a countable number of events (e.g. discontinuous withdrawals). Homogeneity and bang-bangness yield significant reductions in complexity and allow us to rapidly generate numerical solutions. Results are presented which demonstrate the sensitivity of the hedging expense to various parameters. The costly nature of the death benefit is documented. It is also shown that for a typical contract, the fee required to fund the cost of hedging calculated under the assumption that the policyholder withdraws at the contract rate is an appropriate approximation to the fee calculated assuming optimal consumption.
An evaluation of the prevalence of Bovine herpesvirus 1 abortions based on diagnostic submissions to five U.S.-based veterinary diagnostic laboratories

Bovine herpesvirus 1 (BoHV-1) is 1 of several viruses considered to be causal agents of bovine abortion. The current retrospective study examined data on bovine abortion submissions from 5 veterinary diagnostic laboratories from 2000 to 2011. The objective of the study was to describe prevalence of BoHV-1–associated abortion in submissions at veterinary diagnostic laboratories. There were 3 specific aims: 1) to examine the proportion of BoHV-1–related abortions with the introduction of new diagnostic assays such as polymerase chain reaction (PCR), 2) to evaluate the agreement of the histopathology report of the abortion submissions and the result of the assay used, and 3) to evaluate if there was an association between farm history of vaccination against BoHV-1 and BoHV-1–positive abortion submissions. An extended Mantel–Haenszel χ2 for linear trend was used to analyze the prevalence of BoHV-1 over the study period and showed that collectively there is evidence of an increase of positive BoHV-1 abortions (P < 0.001). The comparison of the proportion of BoHV-1–positive submissions pre- and postadoption of PCR was not significant (P = 0.25). Using Cohen kappa coefficient test of agreement, a kappa value of 0.81 (P < 0.001) was found, suggesting high agreement of lesions reported and assay result. It was found that using a χ2 test, a P value of 0.068 for nonmatched data (i.e., a history of vaccination against BoHV-1 in the herd) was associated with reduced detection of BoHV-1–positive abortion submissions.
Diffusion of Innovations and HIV/AIDS

As the HIV/AIDS epidemic continues its relentless spread in many parts of the world, DOI provides a useful framework for analyzing the difficulties in achieving behavior change necessary to reduce HIV rates. The DOI concepts most relevant to this question include communication channels, the innovation-decision process, homophily, the attributes of the innovation, adopter categories, and opinion leaders. The preventive measures needed to halt the transmission of HIV constitute a “preventive innovation. ” This article describes the attributes of this preventive innovation in terms of relative advantage, compatibility, complexity, trialability, and observability. It reviews studies that incorporated DOI into HIV/AIDS behavior change interventions, both in Western countries and in the developing world. Finally, it discusses possible reasons that the use of DOI has been fairly limited to date in HIV/AIDS prevention interventions in developing countries.
Efficacy and safety of entecavir versus lamivudine over 5 years of treatment: A randomized controlled trial in Korean patients with hepatitis B e antigen-negative chronic hepatitis B

Background/Aims Long-term data on antiviral therapy in Korean patients with hepatitis B e antigen (HBeAg)-negative chronic hepatitis B (CHB) are limited. This study evaluated the efficacy and safety of entecavir (ETV) and lamivudine (LAM) over 240 weeks. Methods Treatment-naive patients with HBeAg-negative CHB were randomized to receive ETV 0.5 mg/day or LAM 100 mg/day during the 96 week double-blind phase, followed by open-label treatment through week 240. The primary endpoint was the proportion of patients with virologic response (VR; hepatitis B virus [HBV] DNA<300 copies/mL) at week 24. Secondary objectives included alanine aminotransferase (ALT) normalization and emergence of ETV resistance (week 96), VR and log reduction in HBV DNA levels (week 240), and safety evaluation. Results In total, 120 patients (>16 years old) were included (ETV, n=56; LAM, n=64). Baseline characteristics were comparable between the two groups. A significantly higher proportion of ETV-treated patients achieved VR compared to LAM at week 24 (92.9% vs. 67.2%, P=0.0006), week 96 (94.6% vs. 48.4%, P<0.0001), and week 240 (95.0% vs. 47.6%, P<0.0001). At week 96, ALT normalization was observed in 87.5% and 51.6% of ETV and LAM patients, respectively (P<0.0001). Virologic breakthrough occurred in one patient (1.8%) receiving ETV and 26 patients (42.6%) receiving LAM (P<0.0001) up to week 96. Emergence of resistance to ETV was not detected. The incidence of serious adverse events was low and unrelated to the study medications. Conclusions Long-term ETV treatment was superior to LAM, with a significantly higher proportion of patients achieving VR. Both treatments were well tolerated.
Optimizing nanoparticle selection and drive field properties for simultaneous MPI and hyperthermia

This paper investigates the properties of superparamagnetic iron oxide (SPIO) nanoparticles for simultaneous MPI imaging and hyperthermia therapy. For that purpose, FeraSpin SPIO nanoparticles of varying sizes (FeraSpin XS to FeraSpin XL, with hydrodynamic diameters ranging between 10 nm to 70 nm) with different concentrations (~ μM to mM) are utilized to investigate their MPI and heating efficiencies (i.e., specific absorption rate - SAR) as a function of applied magnetic field. Once the setup is fully functional, our results will provide a proof-of-concept for a potential application of MPI: simultaneous hyperthermia and imaging.
The effect of volleyball playing on the knee extensor mechanism

The knee extensor mechanism was examined in 32 male competitive volleyball players (Group V) and in a control group of 49 young adult males (Group C) to evaluate the effects of previous jumping activity on the knee extensor mechanism. Several variables were re corded by means of a structured questionnaire, and by clinical and radiographic examination. The amount of physical activity from the age of 7 years onward was significantly greater in Group V than in Group C. The incidence of anterior knee pain during the year preceding the examination was higher in Group V (31%) than in Group C (6%;P < 0.01). The most common reason for anterior knee pain in Group V was so-called "jumper's knee. " No significant difference be tween the groups was found in the incidence of clinical symptoms and signs of patellar chondromalacia. There were radiologically detectable soft tissue calcifications at the upper or lower pole of the patella or anterior to the patella in 38% of the subjects in Group V, but no such calcifications were seen in Group C (P < 0.001). However, persistent symptoms did not correlate with the calcifications. Group V subjects had a slight but significant trend to patella alta when compared to Group C (P < 0.05) according to the Blackburne and Peel index.
Evaluation of Enoxaparin Dosing as a Risk Factor for Bleeding in Lung Transplant Recipients

Background: Lung transplant recipients commonly develop complications that lead to anticoagulation. Standard FDA-approved enoxaparin dosing in this population results in a high incidence of above-goal anti-Xa levels, but its association with bleeding remains unclear. Objective: To evaluate the association between enoxaparin dosing and bleeding in lung transplant recipients and assess the relationship between dosing and anti-Xa levels. Methods: We conducted a single-center retrospective cohort study of adult lung transplant recipients who received therapeutic enoxaparin between 2000 and 2012 at a tertiary academic center. We dichotomized enoxaparin dosing regimens into standard dose (FDA-approved doses with a 10% rounding margin) and reduced dose. Clinicians ordered anti-Xa levels as deemed clinically appropriate. The primary outcome was major bleeding or clinically relevant nonmajor bleeding. Results: Of 222 patients treated with enoxaparin, 33 (14.9%) had bleeding events, of which half (17/33) were major. Bleeding occurred in 25/146 (17.1%) patients who received standard-dose enoxaparin versus 8/76 (10.5%) patients who received reduced-dose enoxaparin (P = 0.190). Multiple logistic regression demonstrated an independent association between standard-dose enoxaparin and bleeding, after adjusting for confounders (adjusted odds ratio = 3.04; 95% CI = 1.14-8.10). The median enoxaparin dose in patients with above-goal versus at-goal anti-Xa levels was 0.89 versus 0.76 mg/kg every 12 hours; P = 0.006. However, doses yielding at-goal anti-Xa levels had an interquartile range of 0.67 to 0.90 mg/kg, which overlapped with doses yielding above- and below-goal anti-Xa levels. Conclusions: Enoxaparin dose reduction and anti-Xa level monitoring can improve drug safety and facilitate individualized dose optimization in lung transplant recipients.
Detection of chromosomal aneuploidy by interphase fluorescence in situ hybridization in bronchoscopically gained cells from lung cancer patients.

BACKGROUND Development and progression of human malignancies involve multiple genetic changes. New techniques to distinguish neoplastic from benign diseases unequivocally with small amounts of cells as gained by bronchoscopy are needed to come closer to the goal of an early diagnosis in lung cancer. STUDY OBJECTIVE The aim of this study was to determine whether interphase fluorescence in situ hybridization (FISH) can be used to visualize chromosomal aberrations in bronchoscopically gained cells from lung cancer patients and could eventually become a complementary technique to conventional cytology. METHODS We examined 20 cancerous specimens (10 primary tumors, 10 malignant effusions) of 18 lung cancer patients by FISH with DNA probes specific for chromosomes 3, 8, 11, 12, 17, and 18. From five additional patients, endobronchial brushings and/or forceps biopsy specimens were subjected to interphase FISH analysis. RESULTS In all primary tumors and malignant effusions, highly aneuploid cells were detectable by FISH. Chromosomal aberrations always consisted of gains of chromosomal signal numbers, and all chromosomes were found to be aneuploid to a similar extent. Using chromosomal aneuploidy as a marker of malignancy, material obtained by bronchoscopy was then examined for the presence of malignant cells. In all specimens, evidence for malignancy was obtained by FISH, including three specimens in which cells appeared to be normal or reactively changed by cytologic criteria. CONCLUSION We conclude that interphase FISH is useful in detecting aneuploidy associated with malignancy in bronchoscopically gained cells that do not clearly meet the criteria of malignancy by conventional cytologic study.
Development of the PEARLS Model (Particulate Exposure from Ambient to Regional Lung by Subgroup) and Use of Monte Carlo Simulation to Predict Internal Exposure to PM2.5 in Toronto 1

Air pollution is a current and growing concern for Canadians, and there is evidence that ambient levels that meet current exposure standards may be associated with mortality and morbidity in Toronto, Canada. Evaluating exposure is an important step in understanding the relationship between particulate matter (PM) exposure and health outcomes. This report describes the PEARLS model (Particulate Exposure from Ambient to Regional Lung by Subgroup), which predicts exposure distributions for 11 age‐gender population subgroups in Toronto to PM2.5 (PM with a median aerodynamic diameter of 2.5 μm or less) using Monte Carlo simulation techniques. The model uses physiological and activity pattern characteristics of each subgroup to determine region‐specific lung exposure to PM2.5, which is defined as the mass of PM2.5 deposited per unit time to each of five lung regions (two extrathoracic, bronchial, bronchiolar, and alveolar). The modeling results predict that children, toddlers, and infants have the broadest distributions of exposure, and the greatest chance of experiencing extreme exposures in the alveolar region of the lung. Importance analysis indicates that the most influential model variables are air exchange rate into indoor environments, time spent outdoors, and time spent at high activity levels. Additionally, a “critical point” was defined and introduced to the PEARLS to investigate the effects of possible threshold‐pathogenic phenomena on subgroup exposure patterns. The analysis indicates that the subgroups initially predicted to be most highly exposed were likely to have the highest proportion of their population exposed above the critical point. Substantial exposures above the critical point were predicted in all subgroups for ambient concentrations of PM2.5 commonly observed in Toronto after continuous exposure of 24 hours or more.
Overexpression of PrfA Leads to Growth Inhibition of Listeria monocytogenes in Glucose-Containing Culture Media by Interfering with Glucose Uptake

ABSTRACT Listeria monocytogenes strains expressing high levels of the virulence regulator PrfA (mutant PrfA* or wild-type PrfA) show strong growth inhibition in minimal media when they are supplemented with glucose but not when they are supplemented with glucose-6-phosphate compared to the growth of isogenic strains expressing low levels of PrfA. A significantly reduced rate of glucose uptake was observed in a PrfA*-overexpressing strain growing in LB supplemented with glucose. Comparative transcriptome analyses were performed with RNA isolated from a prfA mutant and an isogenic strain carrying multiple copies of prfA or prfA* on a plasmid. These analyses revealed that in addition to high transcriptional up-regulation of the known PrfA-regulated virulence genes (group I), there was less pronounced up-regulation of the expression of several phage and metabolic genes (group II) and there was strong down-regulation of several genes involved mainly in carbon and nitrogen metabolism in the PrfA*-overexpressing strain (group III). Among the latter genes are the nrgAB, gltAB, and glnRA operons (involved in nitrogen metabolism), the ilvB operon (involved in biosynthesis of the branched-chain amino acids), and genes for some ABC transporters. Most of the down-regulated genes have been shown previously to belong to a class of genes in Bacillus subtilis whose expression is negatively affected by impaired glucose uptake. Our results lead to the conclusion that excess PrfA (or PrfA*) interferes with a component(s) essential for phosphotransferase system-mediated glucose transport.
Strategic Leadership and Strategy Implementation: the Case of KCA University

xi CHAPTER ONE: INTRODUCTION . 1 1.1 Background the Study . 1 1.1.1 Concept of Strategy . 3 1.1.2 Strategic Leadership . 4 1.1.3 Strategy Implementation . 5 1.1.4 KCA University in Kenya . 6 1.2 Research Problem.7 1.3 Research Objectives . 9 1.4 Value of the Study . 10 CHAPTER TWO: LITERATURE REVIEW . 11 2.
Negative Regulation of AMP-activated Protein Kinase (AMPK) Activity by Macrophage Migration Inhibitory Factor (MIF) Family Members in Non-small Cell Lung Carcinomas*

Background: Macrophage migration inhibitory factor (MIF) family members promote AMP-activated protein kinase (AMPK) activation in nontransformed cells. Results: MIF family members cooperatively inhibit AMPK in lung adenocarcinoma cell lines. Conclusion: Inhibition of AMPK activation in lung adenocarcinoma cells by MIF family members promotes efficient mTOR activation. Significance: Therapeutic targeting of MIF family members may benefit lung cancer patients. AMP-activated protein kinase (AMPK) is a nutrient- and metabolic stress-sensing enzyme activated by the tumor suppressor kinase, LKB1. Because macrophage migration inhibitory factor (MIF) and its functional homolog, d-dopachrome tautomerase (d-DT), have protumorigenic functions in non-small cell lung carcinomas (NSCLCs) but have AMPK-activating properties in nonmalignant cell types, we set out to investigate this apparent paradox. Our data now suggest that, in contrast to MIF and d-DTs AMPK-activating properties in nontransformed cells, MIF and d-DT act cooperatively to inhibit steady-state phosphorylation and activation of AMPK in LKB1 wild type and LKB1 mutant human NSCLC cell lines. Our data further indicate that MIF and d-DT, acting through their shared cell surface receptor, CD74, antagonize NSCLC AMPK activation by maintaining glucose uptake, ATP production, and redox balance, resulting in reduced Ca2+/calmodulin-dependent kinase kinase β-dependent AMPK activation. Combined, these studies indicate that MIF and d-DT cooperate to inhibit AMPK activation in an LKB1-independent manner.