Miracle-dz/UniFAR-FTUs huggingface dataset:
Permanent Genetic Resources added to Molecular Ecology Resources Database 1 April 2012 – 31 May 2012

This article documents the addition of 123 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Brenthis ino, Cichla orinocensis, Cichla temensis, Epinephelus striatus, Gobio gobio, Liocarcinus depurator, Macrolophus pygmaeus, Monilinia vaccinii‐corymbosi, Pelochelys cantorii, Philotrypesis josephi, Romanogobio vladykovi, Takydromus luyeanus and Takydromus viridipunctatus. These loci were cross‐tested on the following species: Cichla intermedia, Cichla ocellaris, Cichla pinima, Epinephelus acanthistius, Gobio carpathicus, Gobio obtusirostris, Gobio sp. 1, Gobio volgensis, Macrolophus costalis, Macrolophus melanotoma, Macrolophus pygmaeus, Romanogobio albipinnatus, Romanogobio banaticus, Romanogobio belingi, Romanogobio kesslerii, Romanogobio parvus, Romanogobio pentatrichus, Romanogobio uranoscopus, Takydromus formosanus, Takydromus hsuehshanesis and Takydromus stejnegeri.
EARLY AND RELIABLE DIAGNOSIS OF NON-HODGKIN LYMPHOMA in CHILDHOOD AND ADOLESCENCE: Contribution of Cytomorphology and Flow Cytometric Immunophenotyping

Non-Hodgkin lymphoma (NHL) represents one of the most rapidly growing malignancies in childhood and adolescence. About 80% of patients now are cured with adequate treatment. Serious complications at presentation due to tumor lysis syndrome or local tumor effects are commonly observed. Thus, a rapid diagnosis with the least invasive procedure enabling the initiation of early and specific therapy is necessary to diminish early fatality or persistent impairment. In 56 centrally registered patients with NHL, cytomorphologic analyses (FAB criteria) of May-Grünwald-Giemsa–stained touch imprints or malignant effusions and flow cytometric immunophenotyping (EGIL criteria) of fresh cell suspensions with a standardized panel of monoclonal antibodies were performed. The authors identified 23 patients with Burkitt lymphoma by the combination of FAB L3 morphology and a mature B-cell phenotype and 22 patients with lymphoblastic lymphoma by FAB L1/L2 morphology and a T-/B-cell precursor phenotype. They also found 11 patients with large cell lymphomas, 3 of them with anaplastic large cell lymphoma (T-cell phenotype; NPM/ALK-positive). In the remaining 8 patients diffuse large B-cell lymphoma was suspected by the combined use of cytologic and immunophenotypic findings (mature B-cell phenotype). In all cases with available solid tumor material (n = 42/56) the preliminary diagnosis was confirmed by histopathology. Burkitt lymphoma, lymphoblastic lymphoma, and, in a few cases, some large cell lymphomas could be classified reliably by cytomorphology and immunophenotyping of freshly obtained tumor cell material, enabling an early start of specific lymphoma treatment.
Inverse Faraday effect in plasmonic heterostructures

The phenomenon of the surface plasmon assisted electromagnetic field concentration in the vicinity of the metal/dielectric interface of a periodically perforated with subwavelength holes arrays plasmonic structure is considered. An increase of the local stationary magnetic field appearing due to the inverse Faraday effect while the heterostructure is illuminated by circularly polarized light is demonstrated. The stationary magnetic field induced is more than an order of magnitude larger than the one for a uniform non-plasmonic film which implies a possibility for local control of the magnetization.
Assays for apoptosis and complement-dependent cytotoxicity ( CDC ) Apoptosis assays : To investigate the antiproliferative or apoptotic effect of GnRH peptide analogs and GHR 106 Mabs

Volume 1 | Issue 1 Abstract A monoclonal antibody (Mab) designated as GHR106 was generated against the extracellular domain (N1-29 synthetic peptide) of human gonadotropin releasing hormone (GnRH) receptor. It is a first-in-class GnRH analog and can serve as a drug candidate for potential applications in the treatment of human cancers and/or fertility regulations. Both Mabs in murine (mGHR106) or humanized (hGHR106) forms were shown to have comparable specificity and affinity to intact GnRH receptor on cancer cells or to N1-29 synthetic peptides from humans and monkeys. Similar to decapeptide GnRH analogs, both Mabs were shown to induce apoptosis to cultured cancer cells of various tissue origins, including those from the ovary, breast, prostate, and lung. However, both Mabs were also found to induce complement-dependent cytotoxicity (CDC) reaction for lysis of cancer cells, an immune property which is not shared by peptide analogs of GnRH. By using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), both GHR106 Mabs and the GnRH decapeptide antagonist, Antide, were shown to be bioequivalent in terms of their respective effects on genes involved in the proliferation and apoptosis of cancer cells. In addition, GHR106 Mabs have a much longer circulating half-life than GnRH peptide analogs (days versus hours). Based on the results of these studies, it can be concluded that both mGHR106 and hGHR106 are bioequivalent to the GnRH decapeptide antagonist, Antide, in many biological and functional properties. Therefore, hGHR106 can serve as a long-acting alternative to the current decapeptide GnRH antagonists for therapeutic treatments in the immunotherapy of human cancers, including those of gynecologic origin.
Transfusion Rate for 500 Consecutive Liver Transplantations: Experience of One Liver Transplantation Center

Background Orthotopic liver transplantation (OLT) has been associated with major blood loss and the need for blood product transfusions. During the last decade, improved surgical and anesthetic management has reduced intraoperative blood loss and blood product transfusions. A first report from our group published in 2005 described a mean intraoperative transfusion rate of 0.3 red blood cell (RBC) unit per patient for 61 consecutive OLTs. Of these patients, 80.3% did not receive any blood product. The interventions leading to those results were a combination of fluid restriction, phlebotomy, liberal use of vasopressor medications, and avoidance of preemptive transfusions of fresh frozen plasma. This is a follow-up observational study, covering 500 consecutive OLTs. Methods Five hundred consecutive OLTs were studied. The transfusion rate of the first 61 OLTs was compared with the last 439 OLTs. Furthermore, multivariate logistic regression was used to determine the main predictors of intraoperative blood transfusion. Results A mean (SD) of 0.5 (1.3) RBC unit was transfused per patient for the 500 OLTs, and 79.6% of them did not receive any blood product. There was no intergroup difference except for the final hemoglobin (Hb) value, which was higher for the last 439 OLTs compared with the previously reported smaller study (94 [20] vs. 87 [20] g/L). Two variables, starting Hb value and phlebotomy, correlated with OLT without transfusion. Conclusions In our center, a low intraoperative transfusion rate could be maintained throughout 500 consecutive OLTs. Bleeding did not correlate with the severity of recipient’s disease. The starting Hb value showed the strongest correlation with OLT without RBC transfusion.
Risk Faktörleri ve Risk Değerlendirme Modellerinin Farklı Veri Setleri Üzerinde Gerçeklenmesi

Özet. Başarısız giden birçok proje, hatalı yönetilen süreçler, ürünlerin ve projelerin zamanında teslim edilememesi, maliyetlerde oluşan aşırı artışlar yazılım mühendisliğinde risk yönetiminin önemini günden güne arttırmaktadır. Özellikle risklerin düzgün bir şekilde ele alınamaması, projeler arası risk türlerinin ve gruplarının farklılaşması, şirket/kurum içi yapılan risk yönetimi ve risk analizi çalışmalarının ölçülememesi de bu durumu karmaşıklaştırmaktadır. Bu çalışmada risk faktörleri, sınıflandırıcı yöntemleri ve model performans yapıları incelenmekte, ayrıca 2 farklı şirketin veri setlerinin karşılaştırılmasına da yer verilmektedir. Gerçekleştirilen uygulamada, farklı firmaların veri setleri üzerinde faktör kararı verilerek gereksiz risk faktörlerinin projelerden çıkarılması sağlanmış, 32 risk faktörü kendi içinde sıralanarak hangi alt alanın en çok risk taşıdığı ortaya konulmuştur. Veri setleri içindeki risk faktörlerinin etki değerleri, risk faktörlerinin birbirleriyle kıyaslanmaları ve risk faktörü eleme yöntemlerinden bahsedilmiş olup, bu faktörler yardımıyla veriler çok kritik, kritik, koşullu hata, orta seviyeli ve ihmal edilebilir olarak sınıflandırılmıştır. Sınıflandırma işleminde Destek Vektör Makineleri, Karar Ağaçları ve Naive Bayes sınıflandırıcılarından faydalanılmıştır. Kullanılan yöntemlerin başarı oranları ve başarı yüzdeleri de değerlendirilmiştir.
Acute bacterial meningitis in children admitted to a rural Kenyan hospital: increasing antibiotic resistance and outcome

Background. Acute bacterial meningitis (ABM) is an important cause of mortality in Africa, but most studies are based in urban referral hospitals. Poor laboratory facilities make diagnosis difficult, and treatment is limited to inexpensive antibiotics. Methods. We retrospectively reviewed data from children admitted with ABM to a Kenyan district hospital from 1994 through 2000. We calculated the minimum incidence in children admitted from a defined area. We also examined the antibiotic susceptibility patterns. Results. We identified 390 cases (1.3% of all admissions) of whom 88% were <5 years old. The apparent minimum annual incidence in children younger than 5 years of age increased from 120 to 202 per 100 000 between 1995 and 2000 (P < 0.001). Increasing the lumbar punctures performed by including prostrated or convulsing children significantly increased the number of cases detected (P < 0.005). The most common organisms in infants <3 months were streptococci and Enterobacteriaceae . Streptococcus pneumoniae (43.1%) and Haemophilus influenzae (41.9%) were predominant in the postneonatal period. The overall mortality was 30.1%, and 23.5% of survivors developed neurologic sequelae. Chloramphenicol resistance of H. influenzae rose from 8% in 1994 to 80% in 2000 (P < 0.0001) accompanied by an apparent increase in mortality. A short history, impaired consciousness and hypoglycemia were associated with death. Prolonged coma and low cerebrospinal fluid glucose were associated with neurologic sequelae. Conclusion. ABM in rural Kenya is a severe illness with substantial mortality and morbidity. Prognosis could be improved by broadening the criteria for lumbar puncture and use of appropriate antibiotics.
Occurrence and Antibiotic Sensitivity of Listeria monocytogenes Strains Isolated from Oysters, Fish, and Estuarine Water

ABSTRACT We analyzed the presence of Listeria spp. in oyster, fish, and seawater samples and tested isolates for antibiotic sensitivity. Listeria monocytogenes was found in 4.5% of fish samples and 8.3% of seawater samples and was not recovered from oysters. Multiresistant environmental strains were found, representing a potential threat to human health.
Anterior intervertebral disc herniations in children: unrecognised chronic trauma to the spine.

SUMMARY Five new cases of Anterior Intervertebral Disc Herniations are reported. Anterior intervertebral disc hemiation is a common complication of chronic spinal trauma. It is often accompanied by vertebral fractures which present as localised, mild platyspondyly or as frank vertebral body fractures. Two groups of children are prone to develop the disease. I-Healthy boys active in sport and II -Children with osteoporosis. The differential diagnosis includes discitis, tuberculosis, eosinophilic granuloma and specifically Scheuermann's disease.
Design considerations for electroporation reactors

An electroporation reactor comprises a treatment area for the application of pulsed electric fields to biological tissue. A homogeneous opening of cells within the whole treatment area can be achieved by applying a homogeneously distributed electric field of sufficient field strength and also sufficient total energy to the tissue. However, such an ideal homogeneous electric field can be achieved in a closed volume only. Inlet and outlet of an electroporation reactor designed for a continuous flow of material involve inhomogeneities of the electric field. Such inhomogeneities cause additional losses due to current flowing also in low-field regions, which are ineffective for electroporation. Depending on the design of the electrode system also the electric field in the center of the electroporation area might be to some extend inhomogeneous. So the specific energy required for the treatment of a defined volume of material depends on the electrode design. When comparing this specific energy to the specific energy required in the ideal case of a treatment area with a homogeneous field, a factor describing the energy efficiency of the electrode design can be defined. The paper describes this approach in more detail.
The Spatial Structure of Observation Errors in Atmospheric Motion Vectors from Geostationary Satellite Data

Abstract This study investigates and quantifies in detail the spatial correlations of random errors in atmospheric motion vectors (AMVs) derived by tracking structures in imagery from geostationary satellites. A good specification of the observation error is essential to assimilate any kind of observation for numerical weather prediction in a near-optimal way. For AMVs, height assignment, tracking of similar cloud structures, or quality control procedures may introduce spatially correlated errors. The spatial structure of the error correlations is investigated based on a 1-yr dataset of pairs of collocations between AMVs and radiosonde observations. Assuming spatially uncorrelated sonde errors, the spatial AMV error correlations are obtained over dense sonde networks. Results for operational infrared and water vapor wind datasets from Meteosat-5 and -7, Geostationary Operational Environmental Satellite-8 and -10 (GOES-8 and -10), and Geostationary Meteorological Satellite-5 (GMS-5) are presented. Winds fr.
Molecular Analysis of Nipple Fluid for Breast Cancer Screening

Lack of sensitivity and specificity of image-based breast cancer screening has urged the exploration of alternate screening modalities. Nipple fluid, which contains breast epithelial cells, is produced in small amounts in the breast ducts of nonlactating women and can be collected by noninvasive vacuum aspiration. After administration of nasal oxytocin, nipple aspiration yields sufficient material for molecular analysis in the large majority of women. Whereas nipple fluid cytology appears to have only a moderate correlation with breast cancer development, methylation holds promise as a more appropriate biomarker, since methylation aberrations occur as an early and frequent event during carcinogenesis. Using quantitative multiplex methylation-specific PCR, methylation can be detected in minute amounts of DNA extracted from nipple aspirates, precluding the need for more invasive intraductal approaches such as ductal lavage and random periareolar fine needle aspiration. The application of genomic and proteomic diagnostics to nipple aspirates therefore provides unprecedented opportunities for early breast cancer diagnosis amendable to population-based screening.
Magnetoconductance oscillations in graphene antidot arrays

Epitaxial graphene films have been formed on the C-face of semi-insulating 4H-SiC substrates by a high temperature sublimation process. Nanoscale square antidot arrays have been fabricated on these graphene films. At low temperatures, magnetoconductance in these films exhibits pronounced Aharonov–Bohm oscillations with the period corresponding to magnetic flux quanta added to the area of a single antidot. At low fields, weak localization is observed and its visibility is enhanced by intervalley scattering on antidot edges. At high fields, we observe two distinctive minima in magnetoconductance, which can be attributed to commensurability oscillations between classical cyclotron orbits and antidot array. All mesoscopic features, surviving up to 70K, reveal the unique electronic properties of graphene.
Molecular interactions between Anopheles stephensi midgut cells and Plasmodium berghei: the time bomb theory of ookinete invasion of mosquitoes

We present a detailed analysis of the interactions between Anopheles stephensi midgut epithelial cells and Plasmodium berghei ookinetes during invasion of the mosquito by the parasite. In this mosquito, P.berghei ookinetes invade polarized columnar epithelial cells with microvilli, which do not express high levels of vesicular ATPase. The invaded cells are damaged, protrude towards the midgut lumen and suffer other characteristic changes, including induction of nitric oxide synthase (NOS) expression, a substantial loss of microvilli and genomic DNA fragmentation. Our results indicate that the parasite inflicts extensive damage leading to subsequent death of the invaded cell. Ookinetes were found to be remarkably plastic, to secrete a subtilisin‐like serine protease and the GPI‐anchored surface protein Pbs21 into the cytoplasm of invaded cells, and to be capable of extensive lateral movement between cells. The epithelial damage inflicted is repaired efficiently by an actin purse‐string‐mediated restitution mechanism, which allows the epithelium to ‘bud off’ the damaged cells without losing its integrity. A new model, the time bomb theory of ookinete invasion, is proposed and its implications are discussed.
Family Polyvictimization and Elevated Levels of Addiction and Psychopathology Among Parents in a Chinese Household Sample

When studying the co-occurring victimization within a family, current literature often focuses on individual violence and fails to cover more than two forms of violence. This study fills the research gap by using families, instead of individuals, as units and investigating the prevalence of family polyvictimization. Family polyvictimization is defined as the co-occurrence of child victimization, intimate partner violence (IPV) between parents, and elder abuse within a family. This study analyzed a set of data from 7,466 households, with at least a child under 18 years of age, in six regions in China collected during 2009 and 2010. Descriptive analyses and ordinal logistic regressions were performed to explore the prevalence of family polyvictimization, as well as its associations with parents’ addictive behaviors and negative health factors. The lifetime prevalence and the past-year prevalence of family polyvictimization was 2.53% and 1.09%, respectively. Parents from a polyvictimized family were more likely to report addictive behaviors, and to show poorer mental health and more posttraumatic stress disorder (PTSD) and depressive symptoms than those who were less exposed to violence. Findings show the importance of the whole-family approach to screening multiple types of violence within a family when one type is detected, as well as the potential usefulness of identifying at-risk families among parents with addictive behaviors and poor mental health.
A Theory-Driven Design Framework for Social Recommender Systems

Social recommender systems utilize data regarding users’ social relationships in filtering relevant information to users. To date, results show that incorporating social relationship data – beyond consumption profile similarity – is beneficial only in a very limited set of cases. The main conjecture of this study is that the inconclusive results are, at least to some extent, due to an under-specification of the nature of the social relations. To date, there exist no clear guidelines for using behavioral theory to guide systems design. Our primary objective is to propose a methodology for theory-driven design. We enhance Walls et al.’s (1992) IS Design Theory by introducing the notion of “applied behavioral theory,” as a means of better linking theory and system design. Our second objective is to apply our theory-driven design methodology to social recommender systems, with the aim of improving prediction accuracy. A behavioral study found that some social relationships (e.g., competence, benevolence) are most likely to affect a recipient’s advice-taking decision. We designed, developed, and tested a recommender system based on these principles, and found that the same types of relationships yield the best recommendation accuracy. This striking correspondence highlights the importance of behavioral theory in guiding system design. We discuss implications for design science and for research on recommender systems.
Characterization of Di-myo-Inositol-1,1(prm1)-Phosphate in the Hyperthermophilic Bacterium Thermotoga maritima

Di-myo-inositol-1,1(prm1)-phosphate (DIP) is present at a significant concentration ((symbl)160 nmol/mg of protein) in the cytoplasm of the hyperthermophilic bacterium Thermotoga maritima. The concentration of DIP was independent of the pH of the growth medium or the cell growth phase but increased with increasing concentrations of NaCl in the growth medium, reaching a maximum ((symbl)450 nmol/mg of protein) at 0.4 to 0.6 M NaCl. A large-scale purification procedure for DIP which yields approximately 18 g of DIP per kg of cells (wet weight) is described. Purified DIP was stable at 90(deg)C for at least 5 h. The presence of DIP (50 mM) did not increase the stability at 90(deg)C of pure forms of the hydrogenase or pyruvate ferredoxin oxidoreductase of T. maritima, suggesting that DIP is not a general thermoprotectant.
Creating an environmental justice framework for policy change in childhood asthma: a grassroots to treetops approach.

OBJECTIVES The Community Action to Fight Asthma Initiative, a network of coalitions and technical assistance providers in California, employed an environmental justice approach to reduce risk factors for asthma in school-aged children. Policy advocacy focused on housing, schools, and outdoor air quality. Technical assistance partners from environmental science, policy advocacy, asthma prevention, and media assisted in advocacy. An evaluation team assessed progress and outcomes. METHODS A theory of change and corresponding logic model were used to document coalition development and successes. Site visits, surveys, policymaker interviews, and participation in meetings documented the processes and outcomes. Quantitative and qualitative data were analyzed to assess strategies, successes, and challenges. RESULTS Coalitions, working with community residents and technical assistance experts, successfully advocated for policies to reduce children's exposures to environmental triggers, particularly in low-income communities and communities of color. Policies were implemented at various levels. CONCLUSIONS Environmental justice approaches to policy advocacy could be an effective strategy to address inequities across communities. Strong technical assistance, close community involvement, and multilevel strategies were all essential to effective policies to reduce environmental inequities.