Miracle-dz/UniFAR-FTUs huggingface dataset:
Développement d'un pipeline automatisé d'analyse de données de cytométrie de masse pour l'identification de populations immunitaires

La cytometrie en flux a ete pendant longtemps la methode de choix pour etudier le phenotype des cellules immunitaires. Aujourd’hui, la nouvelle technologie de cytometrie de masse offre une alternative puissante car elle permet de mesurer l’expression de trois fois plus de marqueurs simultanement a plus haute resolution. En augmentant le nombre de marqueurs mesures par cellules, l’analyse manuelle des donnees est devenue de plus en plus fastidieuse en plus d’etre dependante de l’analyste. Dans cet article est presente un pipeline d’analyse automatise, rapide et objectif, developpe pour les donnees de grandes dimensions de cytometrie de masse. Il se divise en plusieurs parties : le nettoyage des donnees brutes, le controle qualite, l’identification de sous-populations immunitaires presentes et leur visualisation. Deux approches differentes sont proposees pour l’identification des types-cellulaires et peuvent etre utilisees seules ou en parallele. La premiere est une methode supervisee de gating semi-automatique basee sur l’environnement OpenCyto. La seconde utilise les algorithmes de clustering non supervises FlowSOM et Consensus Clustering pour partitionner des cellules en differentes populations. Celles-ci sont ensuite visualisees grâce a l’algorithme de reduction de dimension UMAP. Ce pipeline implemente sur R sera bientot complete d'une interface graphique R Shiny pour faciliter son utilisation.
Proper observance of the principle of reciprocity as a guarantee for the sustainable development of cooperation between the subjects of cross-border territories in the field of criminal justice: the moral and legal aspect

The article discusses regulatory legal acts, bilateral treaties, agreements governing international cooperation in the field of criminal justice. Based on their analysis and theoretical positions, the concept of “reciprocity” is formulated. And also, taking into account the needs of practice, proposals for the further improvement of legal acts on state cooperation in criminal justice based on the principle of reciprocity have been developed.
Morphodynamics of river and coastal transport of sediments in mega delta basin, Niger Delta Nigeria

Niger delta is recognized as the third mega deltas in the history of mankind. Understanding the sediment dynamics and morphodynamics relationship between the morphology of the bedrocks to the hydrodynamic of its transport sediments is crucial for water bodies’ preservation, conservation, and sustainability. Sediments transportation in Niger delta involves a two-phase flow system in which one phase is considered to be fluid and the other phase is solid. The water bodies is seldom saturated with sediments occasioned by irregular solid waste dumps, erosions debris, and transported at ease without any form of management or control for many years now. Consequently, poor water resources, agriculture and fishery production are at extreme lost as sediment transport and deposition influences great magnitude of useful river dynamics. Therefore, the objective of this study is focused on whether sediment transport has an effect on the morphology of the channel in the Niger Delta basin. It is necessary to determine the relationship between these two key variables; Sediment yield and channel morphology. A correlation matrix was generated in MS Excel to determine the underlying relationship between the variables under study with a coefficient of determination of 11.23% and a positive correlation between suspended sediment yield, bed load and channel morphology of +0.345. The paper will be useful to research institutions, water resources agencies, government and environmental experts and engineers.
Estimation of work-related back pain syndrome in emergency medical services personnel

Introduction. Emergency medical services personnel is an occupational group exposed to the occurrence of musculoskeletal disorders in relation to the work performed, including back pain syndrome (BPS). The most frequent causes of BPS are unfavourable environmental factors, repeated patient handling during the day, and forced body positions at work. Objectives. The aim of the study was to assess the occurrence of BPS in relation to the work performed and its impact on functional disability in paramedics. Materials and method. The study included 70 emergency medical services personnel aged 24–56 years working in shift mode in Greater Poland. The survey included questions about socio-demographic data, characteristics of the work of paramedics, intensity of back pain according to the Visual Analogue Scale (VAS) scale and level of disability on the Oswestry Questionnaire. Results. Every paramedic declared the occurrence of BPS. The majority (61%) felt pain in the spine a few times a month, most often (65%) in the lumbosacral segment. BPS in more than half (56%) of the respondents caused moderate pain on the VAS scale. The level of disability in 70% of respondents was minimal. Intensity of back pain had the greatest impact on functional limitations in the daily life of the subjects (p <0.05). Conclusions. In the occupational group of paramedics, the problem of the prevalence of BPS is common. In order to reduce back strains during work, it is recommended to use Adres do korespondencji: Monika Kowalczyk, Pracownia Elektrodiagnostyki Medycznej, Katedria i Zakład Profilaktyki Zdrowotnej, Uniwersytet Medyczny w Poznaniu, Przybyszewskiego 49, 60-355, Poznań, Polska E-mail: monikakowalczyk@ump.edu. pl Nadesłano: 04.09. 2019; zaakceptowano do druku: 20.11. 2019; publikacja online: 13.12. 2019 Medycyna Ogólna i Nauki o Zdrowiu 2020, Tom 26, Nr 1, 66–71
Low-Enthalpy Geothermal Springs for Power Generation—An Alternative Approach

The Earth is under increasing stress owing to carbon emissions. Clean energy forms constitute an area of increasing technological interest. Geothermal energy is a clean alternative energy form that can be harnessed from the Earth’s core. The use of geothermal energy resources is gaining substantial interest in many countries, as an integral part of 21st century clean energy solutions and meeting stringent emission requirements. The objective of this research is to investigate an alternative approach to clean and renewable geothermal energy utilization through geothermal ground water pressure applied within a water turbine. The groundwater pressure from the candidate geothermal sites considered is applied to drive a water turbine for the generation of electrical energy. The geothermal candidate sites are low-temperature (i.e. e) than when using low geothermal temperature (0.005 MWe).
Ex ante evaluation of societal impact in research: Towards a conceptually-based reflection

The idea of creating societal impact from publicly funded research has grown in the last decade from a relatively fringe concern related to high-technology entrepreneurship and spin-off companies to an increasingly dominant element of public policy demands for research. This has in turn stimulated a growth in academic science policy research looking at research impact and seeking to conceptualise the ways in which knowledge creation in academic contexts can be coupled to real world outcomes. The bulk of this research has focused on ex post impact, the impacts created by individual research activities or streams after their completion, and there has been much research on ex post impact evaluation. However, evaluating impact ex ante – in research proposals – is becoming increasingly important to funding decisions, and there is very little knowledge relating to what constitutes good impact in a planning proposal. Achieving effect ex ante impact evaluation is necessary to ensure that resources flow to researchers who are planning to create impactful research, and thereby encouraging all researchers to deliver impactful research. In this paper, we develop a conceptual framework for evaluating research impact ex ante, noting that a research proposal constitutes a promise to create future impact. We argue there are two dimensions by which proposals make credible promises, by proposing activities that couple their knowledge with users, and also by making those coupling elements dependencies within the overall project proposal. On this basis, we propose an overall conceptual framework for evaluating research proposal impact ex ante, and suggest an analytic framework for exploring this empirically.
The effects of a passive exoskeleton on muscle activity and metabolic cost of energy

ABSTRACT Nowadays, low back pain has a high incidence during the crowd who work under a long time and repeated stoop condition. Exoskeletons may form a new strategy to reduce the risk of developing low back pain in stressful jobs. A passive exoskeleton device was introduced in this article to apply to the static maintenance of forward bending work, so as to reduce the load of back muscles when wearers work, which plays the role of prevention and subsequent rehabilitation of low back pain. The hypothesis is that these systems would decrease the severity and number of work-related back injuries, while enhancing workers’ safety. Through a preliminary analysis and a substantial number of experiments, we have demonstrated the feasibility of our approach to substantially decrease the forces and muscle activities at the L5/S1 location during bending. In the static holding experiment, we find lower muscle activity (by 35%∼61%) and lower metabolic cost of energy (by 22%) when wearing the exoskeleton. The results demonstrate good potential of this passive exoskeleton to reduce muscle activities at the L5/S1 location and metabolic cost of energy. GRAPHICAL ABSTRACT
Effects of Strenuous Physical Exercises on Blood Redox Status and Muscle Damages among Trained and Untrained Individuals

Several studies suggest that unaccustomed and exhaustive physical exercises could increase the production of reactive oxygen and nitrogen species (RONS) that cause several damages such as lipid, DNA and protein oxidation in blood as well as other cells. High levels of RONS could also play a major role in cardiovascular dysfunction, impair the immune system, reduce immune-surveillance and increase the risk of some cancers.
Longitudinal research on EFL teacher professional development in (Japanese) contexts: Collaborative action research projects

This study examined how secondary teachers (junior and senior high school teachers) of English as a foreign language (EFL) in Japan participated in collaborative action research and engaged in their professional development over four years. In particular, little is known as to how EFL teachers challenge their beliefs and implement innovative practices based on communicative language teaching (CLT) through teacher learning processes. Using a mixed methods design, the study identified three developmental stages regardless of years of teaching experience: (1) Challenging teacher beliefs through TESOL classes and modeling teachers who have done similar action research (AR) through trial and error teaching as they practice communicative activities; (2) Making sense of teaching through adapting and modifying theories of CLT; and (3) Building confidence in teaching by actually seeing students change. These stages overlap and are not mutually exclusive, depending on teacher and teaching context. The study reveals teachers’ dynamic learning processes and professional development.
SANEAMENTO BÁSICO versus DOENÇAS DE VEICULAÇÃO HÍDRICA NO MUNICÍPIO DE PELOTAS/RS

Apesar do avanco do Brasil nos ultimos anos, ainda ha serios problemas com a deficiencia do servico de saneamento basico no pais, principalmente em relacao a coleta e tratamento de esgoto. Diante isto, este estudo teve como objetivo realizar um levantamento das principais doencas de veiculacao hidrica relacionadas a falta de saneamento basico encontradas no municipio de Pelotas/RS entre os anos de 2007 e 2017. Os dados para o presente trabalho foram obtidos junto ao Departamento de Vigilância Epidemiologica de Pelotas e o site do DATASUS para o periodo de 2007 a 2015, para a diarreia e dengue, e 2007 a 2017 para hepatite A e leptospirose. A analise estatistica foi realizada atraves dos valores de medidas de posicao central, medidas de dispersao e das medidas da forma de dispersao. Os valores de Coeficiente de Variacao foram analisados conforme proposto por Wilding; Drees em 1983. Onde foi possivel observar que a diarreia foi a doenca que mais ocorreu em Pelotas durante os anos de 2007 a 2015, seguido da hepatite A, leptospirose e dengue. Conclui-se com este trabalho, a necessidade de um maior investimento por parte dos orgaos responsaveis, em propiciar um saneamento ambiental de boa qualidade a populacao, pois e uma das principais maneiras de evitar contrair as doencas de veiculacao hidrica que foram apresentadas neste estudo. Alem disso, a populacao em geral deve estar atenta e colaborar com a infraestrutura e instalacoes operacionais de abastecimento de agua, esgotamento sanitario, limpeza urbana, drenagem urbana, manejos de residuos solidos e de aguas pluviais, pois as precipitacoes podem interferir negativamente nas ocorrencias das doencas de veiculacao hidrica no municipio de Pelotas, decorrentes da falta de saneamento.
Complex State Variables as Analytical Tool for Control System Design of Medium-Voltage Drives

Complex state variables are used to analyze the dynamics of medium-voltage drives. These operate at low switching frequency to reduce the dynamic losses of the power semiconductor devices. It results in a low sampling rate of the digital signal processing system, causing signal delays that deteriorate the dynamic behavior. Undesired cross-coupling results between the current components id and iq. The effect is shown to be even more adverse than described by the conventional control theory. The solution is modeling the system by single-complex state variables. These permit designing a current controller of high dynamic performance, which exhibits zero cross-coupling. Improved performance at very low switching frequency is demonstrated by experimental results.
Unrelated Cord Blood Transplantation (CBT) for Thalassemia.

CBT is a curative therapy for thalassemia, with cell dosage is a critical factor for CBT. We previously showed that by combining strategies that maximize cell dose-using non-red cell reduced plasma depleted cord blood (PD CB), forego post-thaw wash, and double cord transplantation - promising results may be achieved with unrelated CBT in selected patients. Between 7/91 and 4/06, 45 unrelated CBT were performed after myeloablative therapy (Bu/Cy/ATG in all 33 patients with known regimen) in 36 pediatric patients with transfusion-dependent thalassemia and 2 SCD patients at 14 transplant centers (TC) using 80% PD CB (6 double cords & 1 re-transplant). For the thalassemics, 19 were Lucarelli class 1, 4 class 2, 1 class 3, and 14 status unknown. Median age was 6 yo (range 0.3–20 yo) and median weight was 19 kg (range 8–76 kg). Data was audited. No significant adverse events were observed despite major ABO incompatibility in 9 cases. Unadjusted ANC500, platelet 20K and 50K engraftment with donor chimerism were achieved in 81±7%, 79±8% and 76±8% of the cases, and median times to ANC500 engraftment, platelet 20K and 50K engraftment were at +16.5 (range 11–33), +37 (range 16–133) and +55 (range 26–144 days) days, respectively. 8 patients died including 2 early deaths prior to day +20 and one accidental trauma caused cerebral hemorrhage. All remaining 30 patients are alive, with 25 disease-free and 5 autologous recovery for an 1-Yr OS of 77±7% and DFS at 65±8%. With a mean and median follow up time of 392 and 257 days respectively (range 7–1,760 days), the median day to hospital discharge was day +58 (range 22–137 days). The two experienced TC (>5 cases of CBT for hemoglobinopathy) were compared with less experienced centers, and CBU that were post-thaw washed (14%) were compared with CB that were not washed (86%). Results show potential benefits with TC experience and no post-thaw wash; however, since both experienced centers do not practice post-thaw wash, these two factors cannot be distinguished. Unadjusted ANC500, platelet 20K and 50K engraftment were 87±7%, 86±8% & 86±8% and 1-Yr OS of 87±7% and DFS at 77±9% were achieved for experienced centers not performing post-thaw wash. The role of plasma depletion vs. RBC depletion was also studied by matching age, weight, TNC dose, #HLA matches, conditioning regimen, thalassemia diagnosis, and post-thaw wash between the groups at the 2 experienced TC. Outcome trended towards improvements in engraftment and thalassemia-free survival for PD CB; however, larger numbers and rigorous matched pair analysis are needed for definitive conclusions. Overall, this is the largest known study of unrelated cord blood transplantation for thalassemia and preliminary results show that when cell dose (PD CBU, double cord, and no wash) and other conditions (TC experience and judicious patient selection) are optimal, CBT may be a promising approach for the curative therapy of thalassemia major with unadjusted ANC500, platelet 20K and 50K engraftment rates of 93±7%, 91±8% & 82±8% respectively and 1-Yr OS of 86±9% and DFS at 86±9%.
Knowledge of Cardiovascular Medications in a Culturally Diverse Elderly Community: Health Assessment Outcomes by Nursing Students

Lack of knowledge in medication use appears a major hindrance in managing cardiovascular diseases. The cross-sectional study examined the determinants of knowledge of cardiovascular medications in elderly community, using the survey questionnaire and structured interviews to collect data from 99 culturally-diverse elderly people at independent-living facilities in California. Results indicate that the majority of participants was women (82.8%), living alone with an educational level of high-school or higher. Sixty-six participants took at least one cardiovascular medication, and the average number of cardiovascular medications taken was 2.02 (±1.10). The most frequently used cardiovascular medications were lipid-lowering agents and aspirin. Thirtyeight participants demonstrated a lack of knowledge of cardiovascular medication use. After adjusting for age, gender, education, and living status, it was found that having a BMI higher than 25 (OR: 5.46; 95% CI; 1.12, 26.52), drinking alcohol beverages (OR: 0.075; 95% CI: 0.01, 0.83), and having a history of ever-smoking (OR: 54.90; 95% CI: 4.39, 686.29) were statistically significant, independent predictors of a lack of knowledge about cardiovascular medications.
Why Enjoying Your Fun Matters: The Role of Participation in Fun Activities, Positive Affect, and Citizenship Pressure on Knowledge Management Behaviors

Drawing from social exchange theory, this research develops a mediated-moderation model that examines the direct and indirect effects of participation in fun activities on three knowledge management behaviors (i.e., knowledge sharing, knowledge hiding, knowledge manipulating) and investigates the mediating role of positive affect and the moderating role of citizenship pressure on these relationships. A three-wave, two-source sample (n = 163) of employees belonging to a high-tech start-up in Canada is used to test this model. Results highlight the importance of positive affect by showing the effects of participation in fun activities on knowledge management behaviors is dependent on whether or not participation in fun activities leads to positive affect. Data also shows citizenship pressure moderates the direct relationship between participation in fun activities and knowledge manipulating, as well as the indirect relationship between participation in fun activities and both knowledge sharing and hiding. These results highlight the theoretical and practical importance of both positive affect and citizenship pressure in understanding the dynamic relationship between workplace fun and knowledge management.
From HMI to HMIs: Towards an HMI Framework for Automated Driving

During automated driving, there is a need for interaction between the automated vehicle (AV) and the passengers inside the vehicle and between the AV and the surrounding road users outside of the car. For this purpose, different types of human machine interfaces (HMIs) are implemented. This paper introduces an HMI framework and describes the different HMI types and the factors influencing their selection and content. The relationship between these HMI types and their influencing factors is also presented in the framework. Moreover, the interrelations of the HMI types are analyzed. Furthermore, we describe how the framework can be used in academia and industry to coordinate research and development activities. With the help of the HMI framework, we identify research gaps in the field of HMI for automated driving to be explored in the future.
A Case of Radical Laparoscopic Central Bisegmentectomy with S3 Subsegmentectomy for Multiple Liver Metastases After Chemotherapy

Hepatic resection remains the only potentially curative treatment for patients with colorectal liver metastasis (CRLM) (1). Moreover, only15%-20% of patients with CRLM are suitable for surgical resection (2). Herein wepresent a video case-report of a radical laparoscopic central bisegmentectomy plus S3 subsegmentectomy for multiple liver metastasis after chemotherapy. The patient was a 39-year-old man who was shown to have metachronous multiple liver metastases from sigmoid colon cancer. The size of the tumor in S3 was 7cm, and the size of the tumor in S4/S5/S8 was 13cm. At this point, it was judged that there was no indication for surgery, thuschemotherapy was started. ThemFOLFOX+panitumumabwas administered for 8 courses and FOLFILI+panitumumab was administered for 10 courses. The metastatic lesions showed a partial clinical response to the chemotherapy. Because the tumors were limited in S3 and S4/5/8, a radical hepatectomy was thought to bepossible. A CT scan revealed that the tumor in S3was located near the root of G3, while the tumor in S4/S5/S8 was located near the anterior Glissonean pedicle and partially infiltrated G4. To assure resection of the lesion, weperformed a laparoscopic central bisegmentectomy plus S3 subsegmentectomy. Then, volumetry was performed based on VINCENT CT. The volume of the remnant liver was 41% (Krem=0.077). We confirmed that the operation couldbe performed safely. Because it was necessary to resect a very large area of the liver in this operation, the degree of difficultywas deemed to be very high.To perform this operation successfully, it wasnecessary to secure a good surgical view and make good use of the magnified view afforded via the laparoscope. Thus, acranial-to-caudal direction hepatectomy was performed. The merit of the procedure is that the amount of bleeding is reduced by resecting the liver parenchyma without reversing the direction of the venous branch (3). Even such a difficult operation can be safely performed by devising a thoughtful approach.
Pallister–Killian syndrome with fibular hypoplasia: a novel clinical manifestation

Pallister–Killian syndrome (PKS) is a unique disorder in which affected individuals have tissue mosaics for four copies of the short arm of chromosome 12. We report a case with phenotypic features of PKS despite normal karyotype obtained prenatally. Tetrasomy 12p was identified using fluorescence in-situ hybridization. Among the frequently seen features of PKS in our case, bilateral fibular hypoplasia was found. It is worth mentioning that previous reported cases with PKS did not point to the presence of fibular hypoplasia. This case expands the spectrum of PKS phenotype.