Miracle-dz/UniFAR-FTUs huggingface dataset:
“Football is a boys’ game”: children’s perceptions about barriers for physical activity during recess time

ABSTRACT The aim of the study was to know the factors that influence boys and girls’ perceptions for performing physical activity during playground recess from their own perspective. Ninety-eight schoolchildren aged 8–11 years from five schools from Cuenca (Spain) participated in 22 focus groups and carried out 98 drawings following the socioecological model as a theoretical framework. A content analysis of the transcripts from the focus groups and drawings was carried out by three researchers. Results showed that, in spite of boys and girls identified same barriers, there were gender differences in their perceptions. Gender socialization was the key as central category and helped to understand these differences. Boys preferred play football and this sport had a monopoly on the recess space. Weather was a barrier for boys. Girls and boys, who did not play football, were relegated to peripheral areas and lack of materials was a barrier for them. Teachers were a barrier for all children who did not play football. Thus, in order to promote recess physical activity, researchers, teachers and educational policy makers should take into account gender socialization and promote inclusive non-curricular physical activity in schools.
Spatiotemporal Progress of Nerve Regeneration in a Tendon Autograft Used for Bridging a Peripheral Nerve Defect

We have previously shown that a tendon autograft from the rat tail can support regeneration across a gap in the continuity of the rat sciatic nerve. In this study, we characterized the spatiotemporal progress of regeneration in such a graft bridging a 10-mm defect in the sciatic nerve of the rat. Regeneration was assessed 7, 10, 14, or 18 days postoperatively, by immunocytochemistry for axons, Schwann cells, and macrophages and histochemistry for blood vessels. Axonal regrowth into the grafts showed an initial delay period of 6.8 days, whereafter axons grew at a rate of 1.0 mm/day. Schwann cells grew into the grafts from both the proximal and distal nerve segments, proximally just ahead of the axonal front. Macrophages were initially preferentially located at the periphery of the grafts, but gradually increased inside the grafts. Blood vessels entered the grafts from both the proximal and distal aspects of the severed nerve. The onset of vascularization appeared to coincide with axonal regeneration into the grafts.
Metabolic and clinical response to Escherichia coli lipopolysaccharide in layer pullets of different genetic backgrounds supplied with graded dietary L-arginine.

L-arginine (Arg) is an essential amino acid in birds that plays a decisive role in avian protein synthesis and immune response. Effects of graded dietary Arg supply on metabolic and clinical response to Escherichia coli lipopolysaccharide (LPS) were studied over 48 hours after a single intramuscular LPS injection in 18-week-old genetically diverse purebred pullets. LPS induced a genotype-specific fever response within 4 hours post injectionem. Whereas brown genotypes showed an initial hypothermia followed by longer-lasting moderate hyperthermia, white genotypes exhibited a biphasic hyperthermia without initial hypothermia. Furthermore, within 2 hours after LPS injection, sickness behavior characterized by lethargy, anorexia, intensified respiration, and ruffled feathers appeared, persisted for 3 to 5 hours and recovered 12 hours post injectionem. The varying grades of Arg did not alter the examined traits named above, whereas insufficient Arg reduced body growth and increased relative weights of liver and pancreas significantly. At 48 hours post injectionem, increased relative weights of liver and spleen were also found in LPS treated pullets, whereas LPS decreased those of pancreas, bursa, thymus, and cecal tonsils. Moreover, LPS lowered the sum of plasma amino acids and decreased plasma concentrations of Arg, citrulline, glutamate, methionine, ornithine, phenylalanine, proline, tryptophan, and tyrosine, and increased those of aspartate, glutamine, lysine, 1- and 3-methyl-histidine. Elevating concentrations of dietary Arg led to increasing plasma concentrations of Arg, citrulline, ornithine, and 3-methyl-histidine subsequently. As quantitative expression of LPS-induced anorexia, proteolysis, and the following changes in plasma amino acids, pullets showed a significant decrease of feed and nitrogen intake and catabolic metabolism characterized by negative nitrogen balance and body weight loss in the first 24 hours post injectionem. Pullets recovered from the challenge within the second 24 hours post injectionem and changed to anabolism with re-increased feed and nitrogen intake, positive nitrogen retention, and weight gain. To conclude, present results confirmed that LPS induced numerous metabolic and physiological changes in pullet's genotypes, whereas dietary Arg affected the examined traits only slightly.
In the development of a spanish metamap

MetaMap is an online application that allows mapping text to UMLS Metathesaurus concepts, which is very useful interoperability among different languages and systems within the biomedical domain. MetaMap Transfer (MMTx) is a Java program that makes MetaMap available to biomedical researchers. Currently there is no Spanish version of MetaMap, which difficults the use of UMLS Metathesaurus to extract concepts from Spanish biomedical texts. Our ongoing research is mainly focused on using biomedical concepts for cross-lingual text classification and retrieval [3]. In this context the use of concepts instead of bag of words representation allows us to face text classification tasks abstracting from the language [4]. In this paper we evaluate the possibility of combining automatic translation techniques with the use of biomedical ontologies to produce an English text that can be processed by MMTx.
Orbitofrontal Cortex Function and Structure in Depression

Abstract: The orbitofrontal cortex (OFC) has been implicated in the pathophysiology of major depression by evidence obtained using neuroimaging, neuropathologic, and lesion analysis techniques. The abnormalities revealed by these techniques show a regional specificity, and suggest that some OFC regions which appear cytoarchitectonically distinct also are functionally distinct with respect to mood regulation. For example, the severity of depression correlates inversely with physiological activity in parts of the posterior lateral and medial OFC, consistent with evidence that dysfunction of the OFC associated with cerebrovascular lesions increases the vulnerability for developing the major depressive syndrome. The posterior lateral and medial OFC function may also be impaired in individuals who develop primary mood disorders, as these patients show grey‐matter volumetric reductions, histopathologic abnormalities, and altered hemodynamic responses to emotionally valenced stimuli, probabilistic reversal learning, and reward processing. In contrast, physiological activity in the anteromedial OFC situated in the ventromedial frontal polar cortex increases during the depressed versus the remitted phases of major depressive disorder to an extent that is positively correlated with the severity of depression. Effective antidepressant treatment is associated with a reduction in activity in this region. Taken together these data are compatible with evidence from studies in experimental animals indicating that some orbitofrontal and medial prefrontal cortex regions function to inhibit, while others function to enhance, emotional expression. Alterations in the functional balance between these regions and the circuits they form with anatomically related areas of the temporal lobe, striatum, thalamus, and brain stem thus may underlie the pathophysiology of mood disorders, such as major depression.
Differential expression, and genetic association, of CD58 in Swedish multiple sclerosis patients

We report additional genetic and transcriptomic evidence for the role of CD58 (LFA-3) in multiple sclerosis (MS) susceptibility using a Swedish case-control material, with a result that closely mimics that of De Jager et al. (1). This gene was originally selected for further assessment because of implications in MS susceptibility (2), and because of reduced expression in the cerebrospinal fluid (CSF) of MS patients compared to controls investigated using the Affymetrix Human Genome U133 plus 2 arrays, where 2 probe sets for CD58 detected fold changes of 0.65–0.8 (P = 3.1 × 10−3 and 4.4 × 10−2). The current study included 1,077 patients and 1,217 blood-donor controls, all residing in the Stockholm area and of Scandinavian ancestry. Markers from HapMap Phase II were selected to capture most variance around CD58, and Sequenom methodology was utilized to assess genotypes at 12 SNPs. Association was tested by using logistic regression in R, validating the association of several CD58 SNPs. Most notably, the strongest effect was seen by the correlated (r2 of 0.52) alleles rs10924103G and rs12044852A, in analogy with previous results (1), showing odds ratios of 0.73 and 0.72 in multiplicative models (P = 8.3 × 10−5 and 4.3 × 10−4) and an etiological fraction of 45%. Thus, we are able to contribute to the knowledge of CD58 in MS by, in a Swedish material, reporting lowered expression of this costimulatory molecule in the CSF of MS patients, and confirming the genetic association.
Assessing the Impact of Development Disruptions and Dependencies in Analysis of Alternatives of System-of-Systems

Abstract : The development of a System-of-Systems (SoS) remains a highly challenging endeavor due to the complex interdependencies between systems that often times exhibit managerial and operational independence, yet, must work cohesively to achieve an overarching set of capabilities. Current guidelines set forth by the Department of Defense SoS System Engineering guide presents SoS SE as a set of seven core elements which are connected to the 16 technical and management processes in the Defense Acquisition Guidebook (DAG). This guide, however, and subsequent frameworks (such as the Wave Model), are meant to raise awareness of the key issues and products involved. A need exists to create and mature decision support tools to support the decision making process of evolving SoS architectures; this includes the need for properly assessing the impact that potential disruptions can have, and the analysis of alternatives of SoS constructs. Trades between capability and risk are essential decisions that must be addressed for SoS capability planning. Existing tools for such trades, where they exist, can be ineffective and non-intuitive when size and/or interdependency complexity is high. These features create a tradeoff space between development risk and capability potential of a system. The objective of RT-36 was to explore analytical methods to quantify the impact of system interdependencies in the context of SoS capability development and use this to guide system engineering activity for SoS. A variety of approaches are investigated to provide a means to conduct analysis of alternatives while navigating the decision space that simultaneously considers the potential positive impacts of interdependencies (e.g., SoS capability) as well as the negative impacts (e.g. consequences of disruption in development). The research in RT-36 centered on seven analytical methods that have been adapted to support SoS architecting decisions and systems engineering of constituent systems.
Induction of antisporozoite antibodies by biting of transgenic Anopheles stephensi delivering malarial antigen via blood feeding

We produced a transgenic mosquito expressing a rodent malaria vaccine candidate antigen in the salivary gland. Three tandemly repeated amino acid units from the repeat region of circumsporozoite protein of Plasmodium berghei (PbCS3R) fused to red fluorescent protein (monomeric DsRed) was chosen as a vaccine candidate antigen. Immunoblot and fluorescence microscopic analyses showed the transgene expression in the female salivary gland. The transgene product was released from the proboscis as a component of saliva. The monomeric DsRed‐fusion expression system could be suitable for transgene secretion in the saliva of female mosquitoes. Mice repeatedly bitten by transgenic mosquitoes raised antibodies against P. berghei sporozoites, and the sera had protective ability against sporozoite invasion of human hepatoma HepG2 cells. These results suggest that transgene products are immunogenically active in saliva, and induce the antibodies to malaria parasite. These findings indicate that this technology has the potential for production of a ‘flying vaccinator’ for rodent malaria parasites.
Macrolide resistance and azithromycin failure in a Mycoplasma genitalium-infected cohort and response of azithromycin failures to alternative antibiotic regimens.

BACKGROUND Our aim was to determine the efficacy of 1 g azithromycin and alternative antibiotic regimens in a prospective cohort of Mycoplasma genitalium-infected participants, and factors associated with azithromycin failure. METHODS Consecutive eligible M. genitalium-infected men and women attending the Melbourne Sexual Health Centre between July 2012 and June 2013 were treated with 1 g of azithromycin and retested by polymerase chain reaction (PCR) on days 14 and 28. Cure was defined as PCR negative on day 28. Cases failing azithromycin were treated with moxifloxacin, and those failing moxifloxacin were treated with pristinamycin. Pre- and posttreatment samples were assessed for macrolide resistance mutations (MRMs) by high-resolution melt analysis. Mycoplasma genitalium samples from cases failing moxifloxacin were sequenced for fluoroquinolone resistance mutations. Multivariable analysis was used to examine associations with azithromycin failure. RESULTS Of 155 participants treated with 1 g azithromycin, 95 (61% [95% confidence interval {CI}, 53%-69%]) were cured. Pretreatment MRM was detected in 56 (36% [95% CI, 28%-43%]) participants, and strongly associated with treatment failure (87% [95% CI, 76%-94%]; adjusted odds ratio, 47.0 [95% CI, 17.1-129.0]). All 11 participants who had MRM detected in posttreatment samples failed azithromycin. Moxifloxacin was effective in 53(88% [95% CI, 78%-94%]) of 60 cases failing azithromycin; all failures had gyrA and parC mutations detected in pretreatment samples. Six of 7 patients failing moxifloxacin treatment received pristinamycin, and all were PCR negative 28 days after pristinamycin treatment. CONCLUSIONS We report a high azithromycin failure rate (39%) in an M. genitalium-infected cohort in association with high levels of pretreatment macrolide resistance. Moxifloxacin failure occurred in 12% of patients who received moxifloxacin; all had pretreatment fluoroquinolone mutations detected. Pristinamycin was highly effective in treating macrolide- and quinolone-resistant strains.
Efficacy of decompression and fixation for metastatic spinal cord compression: analysis of factors prognostic for survival and postoperative ambulation.

OBJECTIVE The goals of surgical intervention for metastatic spinal cord compression (MSCC) are prolonging survival and improving quality of life. Non-ambulatory paraplegic patients, either at presentation or after treatment, have a much shorter life expectancy than ambulatory patients. We therefore analyzed prognostic factors for survival and postoperative ambulation in patients surgically treated for MSCC. METHODS We assessed 103 patients with surgically treated MSCC who presented with lower extremity weakness between January 2001 and December 2008. Factors prognostic for overall survival (OS) and postoperative ambulation, including surgical method, age, sex, primary tumor site, metastatic spinal site, surgical levels, Tokuhashi score, and treatment with chemo- or radiation therapy, were analyzed retrospectively. RESULTS Median OS was significantly longer in the postoperatively ambulatory group [11.0 months; 95% confidence interval (CI), 9.29-12.71 months] than in the non-ambulatory group (5.0 months; 95% CI, 1.80-8.20 months) (p=0.035). When we compared median OS in patients with high (9-11) and low (0-8) Tokuhashi scores, they were significantly longer in the former (15.0 months; 95% CI, 9.29-20.71 months vs. 9.0 months; 95% CI, 7.48-10.52 months; p=0.003). Multivariate logistic regression analysis showed that preoperative ambulation with or without aid [odds ratio (OR) 5.35; 95% CI 1.57-18.17; p=0.007] and hip flexion power greater than grade III (OR 6.23; 95% CI, 1.29-7.35; p=0.038) were prognostic of postoperative ambulation. CONCLUSION We found that postoperative ambulation and preoperative high Tokuhashi score were significantly associated with longer patient survival. In addition, preoperative hip flexion power greater than grade III was critical for postoperative ambulation.
The Gregarious Lipid Droplet*

Cytoplasmic lipid droplets were considered until recently to be in the same category as glycogen granules, simple storage sites for energy, waxing and waning as metabolic energy needs dictated, but otherwise inert particles. It has become clear, however, that droplets are much more than isolated storage depots in the cell and that they can skate around on the cytoskeleton, physically interact with several organelles over short or long durations, and be beasts of burden, storing important molecules unrelated to lipids for later use. Although important clues to the panoply of droplet functions have come to light as a result of several proteomics studies, some behavioral qualities of this organelle were apparent from older morphological studies. Droplets are indeed gregarious. In this Minireview, after first considering the basic biochemical properties of lipid droplets, I shall focus on their interactions with other organelles, as manifest by morphological and dynamic studies and hinted at by proteomics. The important functions of droplets in storing and chaperoning proteins are well covered in a recent review (1) and will not be discussed here.
Supervised Factorial Learning

Factorial learning, finding a statistically independent representation of a sensory imagea factorial codeis applied here to solve multilayer supervised learning problems that have traditionally required backpropagation. This lends support to Barlow's argument for factorial sensory processing, by demonstrating how it can solve actual pattern recognition problems. Two techniques for supervised factorial learning are explored, one of which gives a novel distributed solution requiring only positive examples. Also, a new nonlinear technique for factorial learning is introduced that uses neural networks based on almost reversible cellular automata. Due to the special functional connectivity of these networkswhich resemble some biological microcircuitslearning requires only simple local algorithms. Also, supervised factorial learning is shown to be a viable alternative to backpropagation. One significant advantage is the existence of a measure for the performance of intermediate learning stages.
Genetic engineering for virus resistance

Plant virus diseases cause severe constraints on the productivity of a wide range of economically important crops worldwide. In India the Green Revolution ushered in intensive agricultural practices and reduced varietal diversity, resulting in the emergence of viral diseases at an alarming pace in the cultivated crops. Some such diseases, which are especially relevant to India, along with their yield losses, are listed in Table 1. Strategies for the management of viral diseases normally include control of vector population using insecticides, use of virus-free propagating material, appropriate cultural practices and use of resistant cultivars. However, each of the above methods has its own drawback. Rapid advances in the techniques of molecular biology have resulted in the cloning and sequence analysis of the genomic components of a number of plant viruses. A majority of plant viruses have a single-stranded positivesense RNA as the genome. However, some of the most important viruses in tropical countries like India have single-stranded and double-stranded DNA genomes and RNA genomes of ambisence polarity, i.e. genes oriented in both directions. An excellent book is now available on the organization of plant viral genomes. Genome organization, electron-microscopic structures and symptoms caused by some of the viruses, referred to in this review, are briefly illustrated in Figure 1. Concomitantly, tremendous advances have taken place in our understanding of plant–virus interaction in the process of pathogenesis and resistance. This, along with associated advances in the genetic transformation of a number of crop plants, have opened up the possibility of an entirely new approach of genetic engineering towards controlling plant virus diseases. There are mainly two approaches for developing genetically engineered resistance depending on the source of the genes used. The genes can be either from the pathogenic virus itself or from any other source. The former approach is based on the concept of pathogen-derived resistance (PDR). For PDR, a part, or a complete viral gene is introduced into the plant, which, subsequently, interferes with one or more essential steps in the life cycle of the virus. This was first illustrated in tobacco by the group of Roger Beachy, who introduced the coat protein (CP) of tobacco mosaic virus (TMV) into tobacco and observed TMV resistance in the transgenic plants. The concept of PDR has generated lot of interest and today there are several host–virus systems in which it has been fully established. Non-pathogen-derived resistance, on the other hand, is based on utilizing host resistance genes and other genes responsible for adaptive host processes, elicited in response to pathogen attack, to obtain transgenics resistant to the virus. The use of non-PDR type of resistance, even though reported much less in the literature in comparison to PDR-based approaches, holds a better promise to achieve durable resistance. Various aspects of the above topics have been reviewed extensively.
GJI Geomagnetism, rock magnetism and palaeomagnetism Coseismic and postseismic deformation associated with the 2003 Chengkung, Taiwan, earthquake

SUMMARY We use GPS-derived coseismic and postseismic displacements of the 2003M w 6.8 Chengkung, Taiwan, earthquake to examine seismogenic behaviour of the southern Longitudinal Valley. We invert for fault slip on the Chihshang fault, a segment of the Longitudinal Valley fault, based on a simplified layered earth model and a listric-shape fault geometry. Our model infers that maximum coseismic slip of about 0.72 m occurred at a depth of 15 km. Conversely, postseismic slip of about 0.1 m over a 157 d period mainly occurred at depths less than 10 km. We find an anticorrelation between coseismic and postseismic slip distributions. However, the depth profiles of seismicity before and after the main shock are very similar and both show the peak seismicity at 15–25 km depth, consistent with the depth range of large coseismic slip. The potency distribution at depth reveals a coseismic slip deficit at shallow depths, which is compensated by postseismic and interseismic creep associated with the Lichi M´ elange.