Miracle-dz/UniFAR-FTUs huggingface dataset:
Differentiation of Ovarian Cancers From Borderline Ovarian Tumors on the Basis of Evaluation of Tumor Vascularity in Multi–Row Detector Computed Tomography—Comparison With Histopathology

Objective The aim of this study was to evaluate the feasibility of multi–detector row computed tomography (MDCT) in the differentiation between borderline ovarian tumors and ovarian cancer on the basis of tumor morphology and specific features of tumor vascularity in correlation with the results at pathology. Methods A triphasic MDCT protocol was used for the analysis of tumor vascularity. The following features were taken into account: (1) The number of vessels in papillary projections, solid-tissue component, and septa (2 vs >2), (2) serpentine and chaotic configuration of vessels, (3) presence of microaneurysms, and (4) presence of arteriovenous microfistulas. Masses with at least 3 of 4 features were considered ovarian cancer (group A) and masses with 2 features or less as borderline tumor (group B). Radiological findings were compared with results of postoperative pathology. Results Pathologic vessels were found in all 56 patients. Thirty-two patients were included in group A and 24 in group B. The results of pathology were as follows: in group A: 31 malignant tumors, including 31 ovarian carcinomas and 1 benign cystadenoma; in group B: 22 borderline ovarian tumors, 1 benign cystadenoma, and 1 ovarian cancer. Conclusions Morphological evaluation of tumor vascularity in MDCT seems to be an efficient method of differentiating between borderline ovarian tumors and ovarian carcinomas. Because of a small number of cases in the current study, a further research seems justified to confirm our results. The presented MDCT-angiographic criteria showed high sensitivity (97%) and specificity (96%) in differentiation of borderline ovarian tumors and ovarian cancers as compared with pathology. The presented CT-angiographic criteria of malignancy showed an excellent interobserver agreement.
Triple Arthrodesis With Lateral Column Lengthening for the Treatment of Planovalgus Deformity

Background: The rigid planovalgus foot has historically been difficult to correct and maintain in a corrected position with triple arthrodesis (TA). The lateral column lengthening (LCL) is a procedure that corrects the position of the planovalgus foot. Combining the TA with LCL at the calcaneocuboid joint may improve ultimate position after fusion for patients with rigid planovalgus foot deformities. Methods: A retrospective review of all patients who underwent TA with LCL through the calcaneocuboid joint for rigid planovalgus foot deformity was performed. Preoperative and postoperative radiographs were compared for foot alignment by measuring the talo-first metatarsal angle in the anterior-posterior and lateral planes, calcaneal pitch, talo-horizontal angle, metatarsal stacking angle, and medial/lateral column ratio. Clinical outcomes were evaluated for correlation with preoperative and postoperative deformity and surgical indications. Results were evaluated using radiographic and clinic outcome measures developed for TA and LCL. Results: Twenty-nine surgeries were identified with solid fusions occurring in 27 patients by 12 weeks postoperatively. Two patients with cerebral palsy had persistent hindfoot valgus. At an average follow-up of 32 months after surgical intervention, correction of the talo-first metatarsal angle in the AP and lateral planes, calcaneal pitch, and talo-horizontal angles were statistically significant. There were 25 good clinical results with minimal or no pain with activity (86.2%) and 4 poor or fair results with moderate or severe pain (13.8%). There were 26 radiographic successes (89.7%) and 3 radiographic failures (10.3%). Cerebral palsy was associated with a higher rate of radiographic failures (P=0.01). There were 15 total complications in 11 feet (37.9%). These included 4 related to hardware, 3 involving neurological symptoms, 2 related to soft tissues, development of a symptomatic bony prominence in 2 patients, 1 forefoot deformity, 2 nonunions, and 1 case of Achilles tendonitis. Conclusion: Good correction can be obtained and maintained with LCL and TA for rigid planovalgus foot deformity. The procedure is associated with good short-term clinical and radiographic outcomes and improves the position of the foot with diminished risk of recurrent or continued deformity as compared with historical controls. Level of Evidence: Level IV (case series).
Total and prolonged filling of the lungs with Ringer’s solution under extracorporeal lung assist (ECLA) in dogs

Background: Massive alveolar lavage has been used clinically to remove materials accumulated in the alveoli. Recently, filling the lungs with oxygenated perfluorochemical (total liquid ventilation) has been investigated. However, effects of complete and prolonged filling of bilateral lungs with aqueous fluid, such as saline or Ringer’s solution, has not been evaluated, although it is possible to sustain gas exchange without the natural lung by using extracorporeal circulation and an artificial lung (extracorporeal lung assist: ECLA). It is also not known whether the lung can recover gas exchange ability after prolonged fluid filling.
The Pea TCP Transcription Factor PsBRC1 Acts Downstream of Strigolactones to Control Shoot Branching1[W]

The function of PsBRC1, the pea (Pisum sativum) homolog of the maize (Zea mays) TEOSINTE BRANCHED1 and the Arabidopsis (Arabidopsis thaliana) BRANCHED1 (AtBRC1) genes, was investigated. The pea Psbrc1 mutant displays an increased shoot-branching phenotype, is able to synthesize strigolactone (SL), and does not respond to SL application. The level of pleiotropy of the SL-deficient ramosus1 (rms1) mutant is higher than in the Psbrc1 mutant, rms1 exhibiting a relatively dwarf phenotype and more extensive branching at upper nodes. The PsBRC1 gene is mostly expressed in the axillary bud and is transcriptionally up-regulated by direct application of the synthetic SL GR24 and down-regulated by the cytokinin (CK) 6-benzylaminopurine. The results suggest that PsBRC1 may have a role in integrating SL and CK signals and that SLs act directly within the bud to regulate its outgrowth. However, the Psbrc1 mutant responds to 6-benzylaminopurine application and decapitation by increasing axillary bud length, implicating a PsBRC1-independent component of the CK response in sustained bud growth. In contrast to other SL-related mutants, the Psbrc1 mutation does not cause a decrease in the CK zeatin riboside in the xylem sap or a strong increase in RMS1 transcript levels, suggesting that the RMS2-dependent feedback is not activated in this mutant. Surprisingly, the double rms1 Psbrc1 mutant displays a strong increase in numbers of branches at cotyledonary nodes, whereas branching at upper nodes is not significantly higher than the branching in rms1. This phenotype indicates a localized regulation of branching at these nodes specific to pea.
Real-time analysis of RAG complex activity in V(D)J recombination

Significance Recombination-activating genes 1 and 2 (RAG1/2) and high mobility group box 1 (HMGB1) create dsDNA breaks at recombination signal sequences (RSSs), initiating the variable, diversity, and joining [V(D)J] recombination pathway for antigen–receptor gene assembly. We have discovered that RAG complex binds and bends RSS DNA at the heptamer, with different binding modes for 12RSS and 23RSS substrates. Our experimentally derived binding kinetics have established that binding, bending, and synapsis precede catalysis. We have also directly observed the dynamics of the synaptic complex, revealing that RAG1/2 bending places the nonamers at nearly perpendicular orientations and that the RAG:12RSS:23RSS synaptic complex is very stable. We provide a kinetic model that integrates physical binding, bending, and synapsis steps of the RAG1/2 pathway. Single-molecule FRET (smFRET) and single-molecule colocalization (smCL) assays have allowed us to observe the recombination-activating gene (RAG) complex reaction mechanism in real time. Our smFRET data have revealed distinct bending modes at recombination signal sequence (RSS)-conserved regions before nicking and synapsis. We show that high mobility group box 1 (HMGB1) acts as a cofactor in stabilizing conformational changes at the 12RSS heptamer and increasing RAG1/2 binding affinity for 23RSS. Using smCL analysis, we have quantitatively measured RAG1/2 dwell time on 12RSS, 23RSS, and non-RSS DNA, confirming a strict RSS molecular specificity that was enhanced in the presence of a partner RSS in solution. Our studies also provide single-molecule determination of rate constants that were previously only possible by indirect methods, allowing us to conclude that RAG binding, bending, and synapsis precede catalysis. Our real-time analysis offers insight into the requirements for RSS–RSS pairing, architecture of the synaptic complex, and dynamics of the paired RSS substrates. We show that the synaptic complex is extremely stable and that heptamer regions of the 12RSS and 23RSS substrates in the synaptic complex are closely associated in a stable conformational state, whereas nonamer regions are perpendicular. Our data provide an enhanced and comprehensive mechanistic description of the structural dynamics and associated enzyme kinetics of variable, diversity, and joining [V(D)J] recombination.
A Redundant Role of the CD3γ-Immunoreceptor Tyrosine-Based Activation Motif in Mature T Cell Function1

At least four different CD3 polypeptide chains are contained within the mature TCR complex, each encompassing one (CD3γ, CD3δ, and CD3ε) or three (CD3ζ) immunoreceptor tyrosine-based activation motifs (ITAMs) within their cytoplasmic domains. Why so many ITAMs are required is unresolved: it has been speculated that the different ITAMs function in signal specification, but they may also serve in signal amplification. Because the CD3ζ chains do not contribute unique signaling functions to the TCR, and because the ITAMs of the CD3-γδε module alone can endow the TCR with normal signaling capacity, it thus becomes important to examine how the CD3γ-, δ-, and ε-ITAMs regulate TCR signaling. We here report on the role of the CD3γ chain and the CD3γ-ITAM in peripheral T cell activation and differentiation to effector function. All T cell responses were reduced or abrogated in T cells derived from CD3γ null-mutant mice, probably because of decreased expression levels of the mature TCR complex lacking CD3γ. Consistent with this explanation, T cell responses proceed undisturbed in the absence of a functional CD3γ-ITAM. Loss of integrity of the CD3γ-ITAM only slightly impaired the regulation of expression of activation markers, suggesting a quantitative contribution of the CD3γ-ITAM in this process. Nevertheless, the induction of an in vivo T cell response in influenza A virus-infected CD3γ-ITAM-deficient mice proceeds normally. Therefore, if ITAMs can function in signal specification, it is likely that either the CD3δ and/or the CD3ε chains endow the TCR with qualitatively unique signaling functions.
Parent Ratings of ADHD Symptoms in Chinese Urban Schoolchildren

Objective: The objective of this study was to assess the psychometric properties of the Chinese ADHD Rating Scale–IV (ADHD RS-IV): Home Version and to explore parent ratings of ADHD symptoms in a large sample of urban schoolchildren in China. Method: Parents of a representative sample of 1,616 schoolchildren (aged 6-17) in 12 Chinese cities completed the ADHD RS-IV: Home Version. Results: The Chinese ADHD RS-IV: Home Version demonstrated satisfactory internal consistency, test–retest reliability, parent–teacher correlation, discriminant validity, and convergent validity. Factor analysis revealed the DSM-IV two-factor model with “inattention” and “hyperactivity-impulsivity” dimensions, accounting for equal variances. Parent ratings revealed lower/similar scores for Chinese schoolchildren compared with the U.S. study. Conclusion: The ADHD RS-IV: Home Version is a reliable and valid ADHD rating scale in China. The factor structure is similar but not identical to the U.S. study. Normative data reveal cultural differences in some aspects of the parent ratings of ADHD.
Improving Factor Models

Factor-mimicking portfolios typically identify and weight well-diversified basis portfolios. Improving weightings of the basis portfolios so that they are more closely related to the optimal portfolio’s weights enhances the pricing accuracy of parsimonious factor models. In this article, the authors—one of whom was a student of Professor Stephen Ross at the Yale School of Management and later a Ross coauthor—show that this can be achieved with known ex post efficiency criteria, applied with common sense that recognizes when statistical biases are minimal. These alternatives to traditional weightings, such as equal long–short portfolios, help explain the returns of popular anomaly portfolios.
A secure communication architecture in the smart grid

In smart grid, how to establish a communication paradigm among the customers, smart meter and utilities is an important challenge, which requires access control, data encryption, and authentication to ensure message integrity and confidentiality. In this paper, we present a communication architecture based on Attribute Based Cryptosystem to secure the communications in smart grids. In this paper, we present a communication architecture based on Attribute Based Cryptosystem to secure the communications in smart grids. A user can decrypt a ciphertext if and only if it possesses the attributes required by the access structure of the data.
Fast DC component suppression method for phase locked loop

The reasons of measured grid voltage signals including dc components in the grid-tied applications could be the original input signal with dc component, the zero drift of detecting elements and converters, or the grid system fault. When the measured grid voltage has the undesired dc component, the low frequency oscillation would appear in the traditional phase locked loop (PLL) and in consequence induce the inaccuracy of calculated phase angle and amplitude. A traditional method to eliminate the DC component in the measured AC signal is to convert the AC signals to DQ components in the synchronous reference frame and then assume a low-pass filter with the low cut off frequency to filter out the low order harmonics added in the converted dc signals, which however would unavoidably prolong the system response time and is unsatisfied in those applications need fast reaction function. This paper proposes a fast dc component suppression method for the phase locked loop, which can fast filter out the dc component appeared in the sampled AC signal. Moreover, the proposed PLL can keep the fast and accurate calculation of fundamental voltage amplitude and phase angle even though the harmonic distortion occurs. Simulation and experimental results verified the effectiveness of the proposed method.
A New Creative Culture Example: The Case of the ‘What will be Happen News Bulletin’ in North Cyprus

The aim of the current study is to explore the conceptual development of trolling behavior and creative culture on new media in the North Cyprus especially on the 2015 Presidential Election. Cyprus presents a unique opportunity for the investigation of creative language and related issues due to its vibrant and unevolved media systems. Currently, there is no comprehensive study that incorporates the conceptual development of trolling culture in North Cyprus political communication in general. This study is also significant for application of the trolling culture in North Cyprus political communication as an election campaign strategy during the horse-race periods. In total, 23 videos were coded according to codebook with regard to creative language and the characteristics of trolling behavior. In order to provide a conceptual definition of the meaning of troll culture for Turkish Cypriots small survey was conducted by 100 Cypriots. The present data suggests that What Will Be Happen News Bulletin 2015 Presidential Election videos in YouTube channel demonstrated higher creative culture examples, which represent classical trolling behavior. The analysis of small survey and content-analysis suggest that the Mehmet Ekin Vaiz was unique due to being the first application of Turkish Cypriot political communication is adopting a classical trolling behavior in 2015 Presidential Election.
Three essays on waste economic : individuals behavior and publics policies

The observation of a positive trend in the amount of waste in France and in the world has called for studies explaining household sorting behavior. This thesis lies in this perspective and aims at determining how to lead consumers to reduce their waste. We first present a review of the literature analyzing the portfolio of waste management public policies. We discuss the limits of the traditional approach stating that individuals adopt a rational behavior, seeking utility gains. Instead we support the idea that addressing behavioral factors is required for public policies supporting recycling behavior to succeed. In a second step, we investigate the the determinants of sorting behavior by building an original survey on 694 individuals in the PACA region. Our study combines and tests hypotheses first developed by sociologists and psychologists with concepts from behavioral economics. We use a probit model to estimate the probability to adopt a selective sorting behavior. Our empirical analysis shows that social influence negatively impacts recycling. Finally, we complete this study with an agent-based model which seeks to explain the sorting of waste as well as how such behavior is impacted by public policies. Our model considers heterogeneous households whose recycling decision is affected by four elements: individual environmental preferences and self-image, the opportunity cost of a tax on sorting, and the cost of sorting. Three public policies are tested: information, tax and "nudges".
[Membrane-bound carbonic anhydrase (CA IV) in human corneal epithelium and endothelium].

PURPOSE Active HCO3- transport through the corneal endothelial cell layer causes a dehydration of the corneal stroma and is thought to be driven by Na/K- and HCO3(-)-dependent ATPase as well as an electro-genic Na/HCO3- cotransport. Transmembrane bicarbonate transport has also been associated with the recently characterised membrane-anchored isoform of carbonic anhydrase (CA IV) in various tissues. We investigated the localisation of CA IV in human fresh and cultured epi- and endothelium at the light- (LM) and electron-microscopic (EM) level. METHODS Postmortem corneas were obtained within 12 hours of death, stored in formaldehyde and sectioned in paraffin. LM immunohisto-chemistry was performed using the purified gamma-globulin fraction of a polyclonal chicken antibody against CA IV isolated from human kidneys. Epi- and endothelial cell cultures were grown in uncoated flasks under standard conditions and processed both for LM and EM immunohistochemistry using the same antibody. RESULTS Lightmicroscopy of fresh tissue showed membrane staining for CA IV in the whole circumference of the endothelium. Little staining was also observed in some cells of the basal cell layer of the epithelium. Immunohistochemical staining at the EM level was confined to the cell surface of confluent cultures of both epi- and endothelial cells. CONCLUSION The localisation of CA IV to the cell surface of fresh and cultured corneal endothelium suggests the presence of a membrane-bound ion exchange mechanism which may be important for HCO3- transport and corneal hydration. Compromising this mechanism by treatment with local carbonicanhydrase inhibitors may be of clinical importance in selected endothelial disease.
Comparison of the prevalence of KRAS-LCS6 polymorphism (rs61764370) within different tumour types (colorectal, breast, non-small cell lung cancer and brain tumours). A study of the Czech population.

AIMS A germline SNP (rs61764370) is located in a let-7 complementary site (LCS6) in the 3'UTR of KRAS oncogene, and it was found to alter the binding capability of the mature let-7 microRNA to the KRAS mRNA. The aim of the study was to evaluate the frequency of the KRAS-LCS6 variant allele in different cancer types that included patients with colorectal cancer (CRC), breast cancer (BC), non-small cell lung cancer (NSCLC) and brain tumour patient subgroups from the Czech Republic. The occurrence of this genetic variant was correlated with the presence of selected somatic mutations representing predictive biomarkers in the respective tumours. METHODS DNA of tumour tissues was isolated from 428 colorectal cancer samples, 311 non-small cell lung cancer samples, 195 breast cancer samples and 151 samples with brain tumour. Analysis of SNP (rs61764370) was performed by the PCR+RFLP method and direct sequencing. KRAS, BRAF and EGFR mutation status was assessed using real-time PCR. The status of the HER2 gene was assessed using the FISH method. RESULTS The KRAS-LCS6 TG genotype has been detected in 16.4% (32/195) of breast cancer cases (in HER2 positive breast cancer 3.3%, in HER2 negative breast cancer 20.1%), in 12.4% (53/428) of CRC cases (KRAS/BRAF wild type CRC in 10.6%, KRAS mutant CRC in 10.1%, BRAF V600E mutant CRC in 18.5%), in 13.2% (41/311) of NSCLC samples, (EGFR mutant NSCLC patients in 8%, EGFR wild type NSCLC in 12.9%), and 17.9% (27/151) of brain tumour cases. The KRAS-LCS6 TG genotype was not significantly different across the studied tumours. In our study, the GG genotype has not been found among the cancer samples. CONCLUSIONS Based on the findings, it is concluded that the occurrence of the KRAS-LCS6 TG genotype was statistically significantly different in association with status of the HER2 gene in breast cancer. Furthermore, significant association between the mutation status of analysed somatic variants in genes of the EGFR signalling pathway (KRAS, BRAF, EGFR) and the KRAS-LCS6 genotype in colorectal cancer and NSCLC has not been established.
Mesenchymal fibroblast growth factor receptor signaling regulates palatal shelf elevation during secondary palate formation

Background: Palatal shelf elevation is an essential morphogenetic process during secondary palate closure and failure or delay of palatal shelf elevation is a common cause of cleft palate, one of the most common birth defects in humans. Here, we studied the role of mesenchymal fibroblast growth factor receptor (FGFR) signaling during palate development by conditional inactivation of Fgfrs using a mesenchyme‐specific Dermo1‐Cre driver. Results: We showed that Fgfr1 is expressed throughout the palatal mesenchyme and Fgfr2 is expressed in the medial aspect of the posterior palatal mesenchyme overlapping with Fgfr1. Mesenchyme‐specific disruption of Fgfr1 and Fgfr2 affected palatal shelf elevation and resulted in cleft palate. We further showed that both Fgfr1 and Fgfr2 are expressed in mesenchymal tissues of the mandibular process but display distinct expression patterns. Loss of mesenchymal FGFR signaling reduced mandibular ossification and lower jaw growth resulting in abnormal tongue insertion in the oral‐nasal cavity. Conclusions: We propose a model to explain how redundant Fgfr1 and Fgfr2 expression in the palatal and mandibular mesenchyme regulates shelf medial wall protrusion and growth of the mandible to coordinate the craniofacial tissue movements that are required for palatal shelf elevation. Developmental Dynamics 244:1427–1438, 2015. © 2015 Wiley Periodicals, Inc.