Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcriptional occlusion caused by overlapping promoters

Significance Under nitrogen-limiting conditions, the master regulator RpoS protects against stress, but slowed growth is the price cells pay for this protection. Under these conditions, transcription of the gene for the RpoS activator Crl increases. Strikingly, we show that this increase in transcription actually decreases Crl protein levels, thus decreasing RpoS activity and increasing growth rate. This unique regulatory mechanism has as its basis two overlapping promoters. Transcription from the promoter used under nitrogen-limitation prevents transcription of the functional crl mRNA producing instead a long noncoding RNA that has no function. Under conditions where protein synthesis is compromised, this simple, but economical mechanism causes a near-instantaneous reduction in Crl levels and RpoS activity without the need to synthesize a regulatory molecule. RpoS (σ38) is required for cell survival under stress conditions, but it can inhibit growth if produced inappropriately and, consequently, its production and activity are elaborately regulated. Crl, a transcriptional activator that does not bind DNA, enhances RpoS activity by stimulating the interaction between RpoS and the core polymerase. The crl gene has two overlapping promoters, a housekeeping, RpoD- (σ70) dependent promoter, and an RpoN (σ54) promoter that is strongly up-regulated under nitrogen limitation. However, transcription from the RpoN promoter prevents transcription from the RpoD promoter, and the RpoN-dependent transcript lacks a ribosome-binding site. Thus, activation of the RpoN promoter produces a long noncoding RNA that silences crl gene expression simply by being made. This elegant and economical mechanism, which allows a near-instantaneous reduction in Crl synthesis without the need for transacting regulatory factors, restrains the activity of RpoS to allow faster growth under nitrogen-limiting conditions.
Preventing and Profiling Malicious Insider Attacks

Abstract : This report examines previous research on malicious insiders with particular emphasis on the social and psychological factors that may have influenced the attacker and their behaviours. This research also draws on corresponding studies into fraud and espionage in non IT scenarios. A range of preventative measures is presented that approach the problem from personnel, policy and technical perspectives. Given the relative scarcity of research into non-technical aspects of malicious insider attacks, further recommendations are also made to study the malicious insiders, involving both government and academic stakeholders. Such research has the potential to provide further preventative measures.
Center for By-Products Utilization TEMPERATURE EFFECTS ON HIGH-PERFORMANCE CONCRETE By

Based on investigations conducted at the UWM Center for By-products Utilization, two different HPC mixtures were proportioned to have the 28-day compressive strength of 85 MPa. The first mixture (Mix 15P) contained 9% Class C fly ash and 14% silica fume and the other mixture (Mix 15E) contained 25% Class C fly ash, 17% Class F fly ash, and 6% silica fume by weight of cementitious materials. Two types of curing methods, standard moist-curing and Variable Temperature Curing Environment (VTCE), were used. For the VTCE, temperature was varied from 29 ± 3oC for 12 hours each day to 41 ± 3oC for the remaining 12 hours each day, to simulate hot weather curing. Tests were conducted to study the influence of temperature on compressive strength, resistance to chloride-ion penetration, air and water permeabilities, and sulfate attack. At 28 days, compressive strengths of the mixtures were 99 MPa and 100 MPa for Mix 15P, and 84 MPa and 88 MPa for Mix 15E for the moist-cured and VTCE-cured specimens, respectively. Both HPC mixtures show excellent performance with respect to compressive strength, resistance to chloride-ion penetration, sulfate attack, and alkali-silica reaction.
Treatment of malignant pleural effusion: PleuRx catheter or talc pleurodesis? A cost-effectiveness analysis.

INTRODUCTION Malignant pleural effusions (MPEs) complicate many advanced malignancies and the median prognosis for those who develop MPEs is 6 months. These effusions lead patients to suffer from significant dyspnea, which may consequently impair mobility and lead to reduced quality of life. There are several treatment options for those with MPE. Thoracentesis may be quick and relatively easy to perform, but has a high recurrence rate; chest tube placement with talc slurry is quite effective at achieving pleurodesis, but this procedure can be quite painful and requires hospitalization. An alternative option is outpatient placement of the Pleurx catheter (Denver Biomedical Inc., Denver, CO) for home-based drainage of effusions. OBJECTIVE To determine the incremental cost effectiveness of treating MPE with talc pleurodesis versus placement of Pleurx catheter. METHODS We used decision analysis to compare treatments for the management of MPE. Cost data for Pleurx and talc treatments were obtained using Medicare reimbursement data for 2008, and outcome data (probability of treatment success and/or complication, and utility of health states) were obtained through literature review. RESULTS Under our base-case analysis, treatment with talc was less costly than Pleurx (talc, $8170.80; Pleurx, $9011.60) with similar effectiveness (talc, 0.281 quality adjusted life years [QALYs]; Pleurx, 0.276 QALYs). Pleurx became more cost effective (<$100K/QALY) when life expectancy was 6 weeks or less. CONCLUSION The treatment choice (talc pleurodesis or Pleurx catheter) for those with an MPE and a prognosis of 6 months should be based on the clinical situation and patient preferences, as well as local expertise and success rates of the procedures. A prospective study specific to the palliative care population might help to clarify which treatment is more cost effective in this population in which optimizing quality of life is essential.
On teaching always evolving wireless technologies

Teaching on wireless telecommunication networks faces a number of challenges, such as the fact that contents are dynamic in nature, the resulting framework is complex and heterogeneous, design principles can largely vary from one system to another, etc. In order to cope with these challenges, novel and advanced methodologies have to be implemented so that the expectations placed by students and industry are met. In this context, this paper will stress the relevance of exploiting the always-evolving wireless technologies to introduce concepts to students in a non-disruptive way, so that novel concepts associated to newly introduced technologies can be presented on top of previous concepts associated to previously existing technologies. In addition, the integration of theoretical contents and practical experience as key enablers of the learning process will also be stressed.
Static and dynamic ultrasonography for the early diagnosis of canine hip dysplasia.

OBJECTIVE To determine the feasibility of sonographical examination of hip joints in non-sedated puppies and to assess the value of static and dynamic ultrasonography for the early diagnosis of canine hip dysplasia (CHD). METHODS Prospective study was carried out. Five hundred and sixty-six puppies between the ages of 16 and 49 days underwent sonographical determination of the α-angle, joint laxity and distraction value (DV). Sonographical parameters were correlated to conventional radiographic CHD classification in the adult dogs. RESULTS α-Angles between 74° and 89° (X±sd=82·8°±2·31°) were measured. Despite the feasibility of sonographical hip assessment in puppies, no statistically significant correlation was found between sonographical determinations of the α-angle, joint laxity, DV and conventional radiographic hip joint classification at the age of 12 to 24 months. CLINICAL SIGNIFICANCE Results of our study suggest that static and dynamic ultrasonography of hip joints in puppies between 16 and 49 days of age is technically feasible but cannot be recommended for detecting puppies that will develop CHD between the ages of 12 and 24 months.
Effect of Vibrio cholerae neuraminidase on the phagocytosis of E. coli by macrophages in vivo and in vitro.

The mechanisms by which Vibrio cholerae neuraminidase VCN acts as an adjuvant is unclear. We have studied the effect of VCN on the phagocyotsis of E. coli 0111 K58 by macrophages in vivo and in vitro. (1) Using and in vivo intraperitoneal clearance test we have shown that prior injection of VCN (50 units/mouse) into the peritoneal cavity of mice enhances the clearance of the bacteria as efficiently as an optimal amount of opsonizing antiserum. We have also shown that the enhancement of intraperitoneal clearance by VCN is due to phagocytosis and intracellular killing of the organisms by macrophages. (2) VCN does not enhance the i.v. clearance of E. coli o111 K58. (3) VCN promotes the attachment of the organisms to and phagocytosis by peritoneal macrophages in vitro. Data are provided which suggest that the promotion of attachment and phagocytosis by VCN cannot be explained by a mere increase of passive absorption of the bacteria to the cell wall.
DNA Diagnosis in Oncology

Abstract Our understanding of the genetic changes that transform normal cells into malignant cells is increasing at a remarkable pace. Some of the changes involve detectable cytogenetic aberrations, whereas others are identifiable only at the molecular level. Many of the structural cytogenetic changes have also been defined molecularly because the genes involved have been cloned. Thus, we are approaching the stage in which the DNA obtained from tumor tissue can be analyzed to detect the presence of a series of chromosomal and molecular alterations. These studies provide important information regarding the precise diagnosis of the tumor type as well as prognostic information about the likely response to therapy and survival.
PENGARUH LEADER MEMBER EXCHANGE (LMX) DAN KEADILAN ORGANISASI TERHADAP KOMITMEN ORGANISASIONAL (Studi empiris pada karyawan Rumah Sakit Bhakti Asih Brebes)

The large number of existing hospitals are at the core of the community, making the community has many options to do health care. There fore the leadership of the hospital and the soldiers must work together providing excellent service to it is maximum patient. Good relations between superiors and subordinate in the hospital an justice, then it would be increase organizational commitment of employees to the hospital. This research to find out how the influence of leader member exchange (LMX) and the organizational justice of organizational commitment. The research was carried out using multiple linear regression analysis wih the help of SPS program 21. The sample in this research are permanent employees of 63 respondent by the census method. Method of data collection in this research is through aqustionnaire distributed to 63 employees remain Bhakti Asih hospital in Brebess. Result of the analysis show that variable leader member exchange (LMX) influential organizational commitment to wards positif and influential organization justice positive variables of organizational commitment with the coefficient of determination Adjusted R Square shows the value of 0,552. The results of the determination of the contribution of leader member exchange (LMX) and justice organizationals in influencing organizational commitment 55,2% unted to 44,8% while the restis affected by other variables
Freight Transportation Derivatives Contracts: State of the Art and Future Developments

In lean and demand-responsive logistics systems, orders need to be delivered rapidly, accurately, and reliably, even under demand uncertainty. Increasing burdens on the industry motivate the introduction of new methods to manage transportation service contracts. One way to hedge transportation capacity and cost volatility is to create derivative contracts. To date, ocean transportation is the only mode of transportation where this type of contract has been applied. The purpose of this article is to provide an overview of freight transportation derivatives and to discuss some recent relevant research. We start by introducing the theory underlying derivative contracts, before reviewing the development of derivatives markets in the maritime industry. We then investigate the adoption of derivatives contracts in trucking (the dominant mode of freight transportation), which we call truckload options. A numerical example follows to illustrate how derivatives could help financially both shippers and carriers. Finally, this article discusses the conditions necessary for the emergence of a market for truckload options.
Novel peptide pancreastatin: Its occurrence and codistribution with chromogranin a in the central nervous system of the pig

The distribution of pancreastatin immunoreactivity was investigated in porcine brain, spinal cord, dorsal root ganglia, and pituitary. In the brain, immunoreactive cell bodies were present in many areas including the cortex, basal ganglia, hippocampus, thalamus, hypothalamus, mesencephalic reticular formation, cerebellum, and medulla oblongata. Immunoreactive fibres were most abundant in the globus pallidus, stria terminalis, entopeduncular nucleus, hippocampus, and in the substantia nigra. In the spinal cord, immunoreactive cells were found in laminae IV±IX. Immunoreactive fibres were concentrated in the dorsal horn. Pancreastatin immunoreactivity was localised to fibres and small cells (5–10% of the total) in the dorsal root ganglia. In the posterior pituitary, many immunoreactive fibres were present and in the anterior lobe subsets of gonadotrophs and thyrotrophs were pancreastatin‐immunoreactive. The localisation of pancreastatin showed a parallel distribution with chromogranin A. Coexistence of pancreastatin with calcitonin generelated peptide (CGRP) immunoreactivity in cell bodies in the spinal cord, including motoneurones, and with CGRP or galanin immunoreactivities in dorsal root ganglion cells was also noted. The differential pattern of pancreastatin immunostaining was reflected in the extractable levels of peptide with highest concentrations in the cortex (55.8 ± 6.0 pmol/g wet weight, mean ± S.E.M.), thalamus (60.0 ± 5.0 pmol/g), hypothalamus (54.4 ± 6.5 pmol/g), and anterior pituitary (2,714 ± 380 pmol/g). Characterisation of pancreastatin immunoreactivity in the hypothalamus and pituitary by gel permeation and high‐pressure liquid chromatography revealed multiple molecular forms, one of which was indistinguishable from natural porcine pancreastatin. The widespread distribution of pancreastatin immunoreactivity suggests this peptide may play a part in several neuroendocrine, autonomic, somatic, and sensory functions, and its colocalisation with chromogranin A is consistent with a precursor‐product relationship.
Polyamine oxidase‐mediated killing of African trypanosomes

African trypanosomes, Trypanosoma brucei brucei, T. vivax and T. congolense, were killed when incubated in vitro with ruminant sera in the presence of exogeneous spermidine, and were non‐infective for mice. Purified polyamine oxidase in the presence of spermidine‐mediated similar killing of trypanosomes. The abundance of polyamine oxidase activity in ruminant sera which can react with polyamines to produce products with cytotoxic properties may explain the trypanosome killing. This system may contribute to non‐specific parasite killing in vivo.
The Determinants of Malaysian Land Taxpayers’ Compliance Attitudes

This article analyzes the determinants of Malaysian land taxpayers’ compliance attitudes. While income taxpayers often have the structural opportunity to underreport income/overstate deductions, it is more difficult to hide land ownership. Despite this, there are high levels of uncollected land tax revenue in Malaysia. We document the factors associated with land taxpayers’ compliance attitudes and our results should be useful to policy makers in Malaysia and elsewhere, as we find that independent variables significant in prior income tax compliance research also extend to the field of property and land tax compliance.
AUDIT DELAY AND THE TIMELINESS OF CORPORATE REPORTING: MALAYSIAN EVIDENCE

This paper investigates the determinants of audit delay in Malaysia. The sample comprises 100 companies listed in Kuala Lumpur Stock Exchange during the period 1996-2000. Descriptive statistics indicates the audit delay is more 100 days for the five years under study with a minimum standard deviation of 36 days. Eight hypotheses, relating audit delay to company size, industry classification, sign of income, extraordinary item, audit opinion, auditor, year-end and risk are tested in this study. Result from t-test of differences, chi-square test of independent and ordinary leas square regression (OLS) largely support the alternate hypotheses put forward except for extraordinary items and company size. The primary findings are that audit delay is significantly longer for company that (1) non-financial industry, (2) receive other that unqualified audit opinions, (3) have other than 31 December as financial year end, (4) audited by non bigfive, (5) incurred negative earnings and (6) have higher risk. It is hoped that this study, which is conducted in an economically and culturally different context from all existing studies, can contribute toward the current literature on audit delay.
Adaptive decision feedback detection with parallel interference cancellation and constellation constraints for multiuser multi-input-multi-output systems

In this study, a novel low-complexity adaptive decision feedback (DF) detection with parallel DF (P-DF) and P-DF constellation constraints (P-DFCC) is proposed for multiuser multi-input–multi-output (MIMO) systems. The authors propose a constrained constellation map which introduces a number of selected points served as the feedback candidates for interference cancellation. By introducing a reliability checking, a higher degree of freedom is introduced to refine the unreliable estimates. The P-DFCC is followed by an adaptive receive filter to estimate the transmitted symbol. To reduce the complexity of computing the filters with time-varying MIMO channels, an adaptive recursive least squares algorithm is employed in the proposed P-DFCC scheme. An iterative detection and decoding (Turbo) scheme is considered with the proposed P-DFCC algorithm. Simulations show that the proposed technique has a complexity comparable to the conventional P-DF detector while it obtains a performance close to the maximum-likelihood detector at a low to medium signal-to-noise ratio range.
Monoamine oxidase A (MAOA) and antisocial behaviors in the presence of childhood and adolescent maltreatment

There is a robust relationship between the experience of maltreatment in childhood and later antisocial behaviors amongst adolescents and adults. Animal and human studies suggest that variation in monoamine oxidase A (MAOA) genotype may moderate the effects of maltreatment. Self‐reported conduct problems and criminal convictions amongst sibling‐pairs from the National Longitudinal Study of Adolescent Health were tested for association with reports of maltreatment before and after the age of 12. MAOA promoter polymorphisms were tested for possible moderation effects. Maltreatment predicted conduct problems and criminal convictions. MAOA genotype did not have a significant moderating effect in any of the six analyses that were conducted. We did not replicate a previous report that MAOA polymorphisms moderated the relationship between maltreatment and conduct problems. There was, however, a non‐significant trend in the predicted direction. Additional studies will be needed before firm conclusions can be drawn about this hypothesized genotype–environment interaction. © 2005 Wiley‐Liss, Inc.
A framework for cloud adoption from Enterprise Architecture and Business Perspective. Validation case study: The implementation of cloud-based contact distribution in Philips Consumer Lifestyle.

The gap between business expectations and actual information technology (IT) delivery is not uncommon. There is an increasingly perceived vision that cloud computing will be able to solve this gap by addressing business demands with faster, easier, and more flexible IT services. However, to achieve these high expectations, firms usually face significant obstacles while creating a holistic cloud strategy. This situation gives the motivation for this research to define a framework that will help the successful implementation of cloud solutions. This thesis aims to propose a framework for cloud adoption which is reusable and addresses the full cycle, starting from the initiation planning until the project delivery from both the enterprise architecture and business point of view.
Curative effect of emergency care for patients with cerebral haemorrhage in Emergency Department

Objective: To discuss the nursing effect of patents with cerebral haemorrhage in emergency department. Methods: One hundred and twenty patients with cerebral haemorrhage were selected from July 2014 to July 2015 and randomly divided into a control group and an observation group according to case number. Patients in the control group were given conventional nursing, while patients in the observation group were treated by predictive nursing intervention measures besides conventional nursing. The clinical effect, incidence of complications, nursing satisfaction and prognosis were compared between the two groups. Results: Compared to that of the control group, the incidence of complications of the observation group was much lower, and the difference had statistical significance (P<0.05); the nursing satisfaction degree of the observation group was higher than that of the control group, and the difference had statistical significance (P<0.05). Fugl-Meyer Assessment scale (FMA) score, Barthel Index (BI) and National Institutes of Health Stroke Scale (NIHSS) score of patients in the observation group were superior to those of patients in the control group, and the differences were statistically significant (P<0.05); the prognosis of the observation group was also better than that of the control group, and the difference was remarkably significant (P<0.05). Conclusions: In the process of emergency treatment for patients with cerebral haemorrhage, the application of predictive nursing intervention is of great significance in lowering the incidence of complications, improving prognosis and enhancing living quality.
Weight Gain in Zollinger-Ellison Syndrome After Acid Suppression

Objectives Zollinger-Ellison syndrome (ZES) is characterized by hypergastrinemia and gastric acid hypersecretion resulting in peptic ulcer disease, diarrhea, and weight loss. Acid secretion can be controlled with medication, and biochemical cure is possible with surgery. Data on how these interventions affect patients’ weight are lacking. We aimed to determine how medical and surgical acid control affects weight over time. Methods We performed a retrospective cohort study on 60 ZES patients. Acid control was achieved with appropriate-dose proton pump inhibitor (PPI) therapy. Surgery was performed for curative intent when appropriate. Weight change was assessed versus pre–acid control or immediate preoperative weights and expressed as absolute and percent change from baseline at 6, 12, 18, and 24 months. Results A total of 30 PPI-controlled patients and 20 surgery-controlled patients were analyzed. Weight gain was noted at all time points while on appropriate-dose PPI therapy (P < 0.005). Of patients who had surgery with curative intent, weight gain was noted at 12 months (7.9%, P = 0.013) and 18 months (7.1%, P = 0.007). There was a trend toward weight gain seen at all time points in the patients who were surgically cured. Conclusions These data represent a novel description of weight gain after acid suppression in ZES.