Miracle-dz/UniFAR-FTUs huggingface dataset:
Abstract. Forkhead box L2 (FOXL2), a member of the forkhead family of transcription factors, is important in eyelid and ovary differentiation. Although the function of FOXL2 in organogen-

Forkhead box L2 (FOXL2), a member of the forkhead family of transcription factors, is important in eyelid and ovary differentiation. Although the function of FOXL2 in organogenesis has been investigated, the detailed mechanisms by which FOXL2 mediates cellular process remain to be fully elucidated. Few FOXL2-knockout cell lines have been reported, which has limited molecular mechanism investigations. cRISPR is a novel gene editing technique that has been widely used in human genetic diseases. In the present study, FOXL2 was disrupted using clustered regularly interspaced short palindromic repeats (cRISPR), and screening of a stable knockout cell line was performed in human ovarian granulosa KGN cells. Three sites (F404, F425 and F446) around the ATG start codon on the FOXL2 dNA sequence were constructed in a guide RNA lentivirus. Targeting F425 was most efficient, and western blot analysis and DNA sequencing of the resulting cells suggested that both FOXL2 alleles were fully disrupted. In addition, flow cytometry results indicated that the knockout of FOXL2 restricted cell cycle progression at the G0/G1 phase. In addition, the expression levels of cell cycle mediators cyclin d1 and cyclin-dependent kinase 4 were reduced. These results confirmed that FOXL2 disruption in KGN cells is associated with the cell cycle attenuation.
Risk factors for additional port insertion in single-port laparoscopic appendectomy

Introduction Single-port laparoscopic appendectomy (SPLA) was expected to have reduced risk of wound infection, less postoperative pain, and improved patient’s satisfaction with better cosmesis compared with conventional laparoscopic appendectomy (CLA). When SPLA is converted to CLA, the additional incision for another port insertion can lead to a decrease in the surgical advantages and clinical benefit. Aim To evaluate risk factors for conversion to CLA during SPLA. Material and methods Between August 2015 and December 2016, patients who underwent intended SPLA were retrospectively reviewed. Conversion was defined as any insertion of an additional port, and complicated appendicitis was defined as gangrenous or perforated appendicitis, abscess, or peritonitis in preoperative computed tomography. Postoperative complication was defined as any deviation in the routine postoperative course within 30 days postoperatively. Results Of 409 patients, 65 (15.9%) were treated with additional port insertion. The overall complication rate was 1.5% in each group, of which 1 patient developed superficial SSI and 4 patients developed deep surgical site infections in the SPLA group. After univariable and multivariable analysis, old age, male sex, increased serum C-reactive protein (OR = 2.944; 95% CI: 1.433–6.047; p = 0.003), and complicated appendicitis (OR = 3.330; 95% CI: 1.304–8.503; p = 0.012) were significant risk factors for conversion to CLA. Conclusions The conversion rate to CLA was 15.9%. Serum C-reactive protein level and complicated appendicitis were strong predictive factors for conversion from SPLA in acute appendicitis.
Effects of constant low-temperature storage on the performance of a commercial strain of Aphidius colemani

This study determined the effects of cold storage of mummies on the biological characteristics of Aphidius colemani Viereck (Hymenoptera Braconidae), a solitary, koinobiont endoparasitoid of more than 40 species of aphids. The effects of three constant temperatures (5, 7 and 9 °C) at five time periods (5, 10, 15, 20 and 30 days) were evaluated on eight quality control parameters of the parasitoid (adult emergence, sex ratio, time elapse before emergence, adult longevity, length of the hind tibia, flight capacity, percent parasitism and sex ratio of F1 wasps). The results showed that all parameters except the sex ratio of F1 were negatively affected by cold storage duration in comparison to control treatment (25 °C). Based on the adult emergence and sex ratio, A. colemani could be stored for 15 days at 5 and 7 °C and for 10 days at 9 °C but it is important to consider other quality control parameters (time elapse before emergence, adult longevity, adult size, flight capacity and percent parasitism). However, when the mummies of A. colemani were stored for longer than 10 days, their quality criteria were more negatively affected at all three temperatures. The results are discussed in order to facilitate the planning of A. colemani mass rearing and aphid biocontrol programs.
Approach to the construction of the genetic fuzzy clustering algorithm

When you work with a lot of data often face the task of class data mining. One of the common problems is the clustering of data. Of particular interest is the fuzzy clustering, providing more complete information about the structure of data clusters. Offers an improved fuzzy clustering algorithm, which can handle local extremes of the convergence function of the algorithm by integrating the fuzzy c-means algorithm with a genetic algorithm. The composition of the genetic algorithm and the algorithm of a fuzzy c-means clustering has also improved characteristics convergence compared to conventional combinatorial search. Thus, we propose an approach that combines both algorithms that complement each other and compensate for lack of both algorithms separately.
Pre-SMA Gray-matter Density Predicts Individual Differences in Action Selection in the Face of Conscious and Unconscious Response Conflict

The presupplementary motor area (pre-SMA) is considered key in contributing to voluntary action selection during response conflict. Here we test whether individual differences in the ability to select appropriate actions in the face of strong (conscious) and weak (virtually unconscious) distracting alternatives are related to individual variability in pre-SMA anatomy. To this end, we scanned 58 participants, who performed a masked priming task in which conflicting response tendencies were elicited either consciously (through primes that were weakly masked) or virtually unconsciously (strongly masked primes), with structural magnetic resonance imaging. Voxel-based morphometry revealed that individual differences in pre-SMA gray-matter density are related to subjects' ability to voluntary select the correct action in the face of conflict, irrespective of the awareness level of conflict-inducing stimuli. These results link structural anatomy to individual differences in cognitive control ability, and provide support for the role of the pre-SMA in the selection of appropriate actions in situations of response conflict. Furthermore, these results suggest that flexible and voluntary behavior requires efficiently dealing with competing response tendencies, even those that are activated automatically and unconsciously.
Reply to reviewers and editors:

This manuscript presents down-scaling results of SO2 and NOx emissions and concentrations based on the coarse-resolution joint emission inversion results from Part 1. The downscaling approaches used information from TROPOMI NO2 observations, MIX inventories, and VIIRS nighttime light observations. The downscaling results were compared against surface in-situ observations. The impact on regional air quality forecasting is also addressed. The prosed approaches are unique and could contribute to improving regional air quality modeling. I would, however, advise the authors to revise the manuscript. These revisions should be made before the manuscript can be considered for publication. Thanks for the positive comments and constructive reviews. We’ve done our best to address the comments in the revision.
The association of maternal plant-based diets and the growth of breastfed infants

Background: Studies are needed to further understand how different plant-based dietary patterns of mothers relate to infant growth. Thus, we investigated the association between maternal plant-based diets and infant growth in breastfed infants during the first 4 months of life. Methods: This cross-sectional study included 290 Iranian mothers and infants. Maternal dietary intake was assessed using a 168-question validated semi-quantitative food frequency questionnaire (FFQ). Three plant-based diet indices (PDIs) were then created to evaluate dietary intakes. Eighteen food groups were classified in three main categories by scoring method: wholeplant diet, healthy plant diet, and animal and unhealthy plant diet. Results: Participants in the top tertile of unhealthy PDI (uPDI) had a lower intake of potassium,phosphorus, zinc, magnesium, calcium, folate and vitamin C, B1, B2, and B3. The upper tertileof uPDI was associated with stunting at 4-month in infants (uPDI: odds ratio [OR] = 3.27, 95%CI= 1.32, 8.10). There were no significant associations between plant-based diet scores and anthropometric indices, including weight, weight status and head circumference (P > 0.05). Conclusion: In conclusion, higher adherence to uPDI may be associated with stuntingamong Iranian infants. Other PDIs were not associated with anthropometric measures. Future studies are needed to further understand the association between plant-based diets and infant growth.
An online survey of Malaysian long-term e-cigarette user perceptions

INTRODUCTION The use of e-cigarettes (EC) has reached alarming proportions among Malaysians. On a national level, little is known about the profile and perceptions of Malaysian EC users. This study aimed to explore the prevalence of long-term EC usage and its associated factors among EC users in Malaysia. METHODS This nationwide online questionnaire survey was administered among 694 EC users across 13 states and 1 Federal Territory in Malaysia, between January and April 2018. A survey link was e-mailed to EC users that were recruited from an official national vape entity through their Facebook association page. We obtained information on respondents’ sociodemographic characteristics, smoking habits, long-term e-cigarette usage and perceptions of EC use. We estimated long-term EC user prevalence and fitted multivariate regression models to predict factors associated with long-term EC usage. Statistical significance was set at p<0.05. RESULTS Respondents were predominantly Malays (87.6%), aged >30 years (68.1%) and tertiary educated (71%). The majority were employed (93.1%) with a monthly household income of MYR 4000 or less (56.6%). About 84% were former smokers, while 10% were current smokers. The prevalence of long-term EC usage in this study was 82.3%. Most users believed that EC had helped them to cut down tobacco smoking (94.8%), reduced the urge to smoke (88.3%) and ultimately helped them to quit smoking (87.2%). Respondents aged >30 years and those who perceived that EC has helped them stop smoking were significantly more likely to be long-term EC users. CONCLUSIONS Most respondents engaged in EC use to quit smoking. They were more likely to be long-term EC users if they were older and perceived that EC has helped them to quit smoking. This information is valuable for targeted prevention, health promotion and policy regulations.
Acoustic stimulus influences ovipositioning in drosophila melanogaster

Evidence indicates that Drosophila melanogaster Meigen (Diptera Drosophilidae) utilize numerous environmental factors to assess the available resources while simultaneously evaluating protection for their offspring in determining ovipositioning. While a number of physical stimuli have been examined, auditory stimuli has not. If D. melanogaster incorporate sound into their determining schema, it is unknown if such a tone would be perceived as beneficial or threatening. In order to assess their tone preference and to confirm previous findings, 120 female D. melanogaster were tested. Their ovipositioning preferences were measured by counting the amount of eggs laid in areas categorized as On, Near, and Off relative to tone location. A 250 Hz tone was presented either to one side of a forced choice chamber, presented on both sides, or there was no tone. Also within the study, substrates (sucrose, caffeine, and none) were varied. It was found that D. melanogaster laid significantly more eggs under the tone (p < 0.001) compared to controls. When given the option to lay their eggs under tone or on sucrose (a previously determined preference), they chose tone at a significant higher rate compared to sucrose (p < 0.001). This study demonstrates that female D. melanogaster may positively associate 250 Hz tone. The evolutionary implications of such behavior are investigated.
Enzyme Exporlation in Different Types of Muscular Dystrophies

Article History Received: 02.10. 2019 Accepted: 20.10. 2019 Published: 26.10. 2019 Abstract: Muscular dystrophy is a genetic disorder leading to progressive weakness of muscles caused due to dysfunction in or lack of protein in muscle cells. The prevalence of muscular dystrophy has been observed globally and is becoming a critical area of study for better health services. The purpose of the study is to analyze the research strength of muscular dystrophy using bibliographic literature. A quantitative literature analysis was carried out on muscular dystrophy from 1991 to 2015 for assessing the global research trends. This literature-based study was conducted using the documents retrieved from the Science Citation Index using the keywords: Duchenne Muscular Dystrophy (DMD), Becker Muscular Dystrophy (BMD), Congenital Muscular Dystrophy (CMD), Myotonic Dystrophy, Emery-Dreifuss Muscular Dystrophy, Facioscapulohumeral Muscular Dystrophy, Oculopharyngeal Muscular Dystrophy, and Limb-Girdle Muscular Dystrophy. Analysis was done for annual productivity of publication, authorship, collaboration, country performance, citation frequency, characteristics of most cited document, journal productivity, etc.
The Relationship Between Positive Psychological Capital and Job Burnout: A Study of Thai Airways International Flight Attendants

This study investigates the relationship between levels of positive psychological capital (PsyCap) and burnout among Thai flight attendants who are currently working for Thai Airways International. The sample included 305 flight attendants. The results indicate that the perceptions of their burnout level is low (mean = 2.10), and degree of their PsyCap is moderate (mean = 3.73); the aggregate of PsyCapand the burnout levels are found to be significantly negative (r = -0.42). All four components of PsyCap are negatively related to the level of burnout. The paper discusses the future research agenda. The implications of these findings and benefits of developing PsyCap for organisations are also discussed in the light of these findings.
Review and Analysis of mental health reforms in several countries: Implementation, comparison and future challenges

Mental health disorders affect people in all societies. In the past thirty years, countries have launched mental health program reforms to care for people affected by mental disorders, mental well-being and protection of human rights. This review presents a wide range of mental health reforms conducted in different countries, compares barriers, present trends for the future and highlight what can be learned from them. A literature review using Pubmed, Ebsco, world bank.org, OECD, Statistic Times and the WHO Mental Health Atlases for 2001, 2005, 2011, and 2017 databases was conducted. The results are presented in tables highlighting key elements comparing demographic information, healthcare professionals working in the mental health sector, policies, legislation and site of treatment in 19 countries. Crucial information is presented in four main themes: Legislation and Regulations; Mental health policies, plans and programs; Eliminating the custodial approach and stigma; and Deinstitutionalization/priority on community care and networks of care. Most mental health reforms began with deinstitutionalization without fully considering the infrastructure needed for community care, financing and the number of healthcare workers in the mental health sector. When initiating mental health reform policy makers should consider legislation, financial ability and establishing intermediate community services to facilitate rehabilitation.
The evolution of altruism as a sexually selected handicap

It is claimed that sexual selection may favor altruism as a handicap. Here, we challenge these claims by developing a model for sexual selection acting on an altruistic handicap trait. By means of simulations we investigate if altruism can evolve as a sexually selected handicap trait despite the fact that competition for mating opportunities may cause altruists to benefit their competitors. We study two scenarios in which individuals with different fitness benefit from altruistic acts. In the first scenario, the benefits from altruism accrue to individuals with high fitness, whereas in the second they are the same for all individuals independent of their fitness. Simulations show that the evolution of altruism is hampered when all recipients benefit equally, but promoted when fitter recipients benefit more. Thus, dependent on how altruism benefits recipients with different fitness, sexual selection can promote altruism as a handicap.
Categories and Dangers of Social Networking among Nigerian In-school Adolescents

The advent of different Information and Communication Technological (ICT) software has attracted global argument regarding associated consequences. It is due to the perceived effects that this descriptive study ascertained categories and dangers of social media networking among adolescents. A sample size of 607 in-school adolescents was randomly selected. Questionnaire was used as an instrument for data collection. The data collected were analyzed using descriptive and inferential statistics (Mean, Standard deviation and t-test). The major findings revealed that adolescent students are exposed to various categories of social networks such as facebook, twitter, eskimi, myspace, 2go, blogs, email, WhatsApp and youtube. It was revealed that poor study habits, identity theft, sexual solicitation are some of the dangers associated with the use of social media networking among the study sample. Based on these findings, some counselling implications were highlighted and recommendations made.
A CONCEPTUAL MODEL OF THE SCS RUNOFF METHOD

The SCS (or NRCS) method, arising in the 1950’s out of analysis of real data from large numbers of catchments across the USA, is one of the most commonly applied flood estimation methods. It is used in several regions within New Zealand and is the preferred method for the Auckland Region. The original approach was empirical and this paper presents a concept of the physical rainfall-runoff processes for the SCS method. It is based on the variable source area concept for promoting runoff. The variable source concept is based on part of the catchment contributing to runoff at an increasing rate with increasing rainfall. It can be demonstrated that a unique storage function can be defined across a catchment representing the catchment type (land use, soil type etc.). The concept provides a physical meaning for the runoff number (CN), and demonstrates that the storage (S) in the SCS method can be taken to represent the average storage across the catchment. It is anticipated that this concept will aid an understanding of SCS applications and perhaps its use in continuous rainfall-runoff modelling.
Information Sharing in a Competitive Microcredit Market

We analyze contract-level data on approved and rejected microloans to assess the impact of a new credit registry in Bosnia and Herzegovina, a country with a competitive microcredit market. Our findings are threefold. First, information sharing reduces defaults, especially among new borrowers, and increases the return on lending. Second, lending tightens at the extensive margin as loan officers, using the new registry, reject more applications. Third, lending also tightens at the intensive margin: microloans become smaller, shorter and more expensive. This affects both new borrowers and lending relationships established before the registry. In contrast, repeat borrowers whose lending relationship started after the registry introduction begin to benefit from larger loans at lower interest rates.
KNOWLEDGE AND INTELLECTUAL CAPITAL MANAGEMENT: THE COMPETITIVE EDGE IN TODAY'S GLOBAL ECONOMY Akaso, A. A

This study examined the agility, absorptive and resilience capabilities of organizations in accelerating their abilities to create, transfer, and deploy knowledge in order to meet organizational current and future performance capacity. The report deployed a descriptive research method. The research finding revealed that management of organizational change is a challenging one that firms must study continually in order to identify the skills set and needed to cope with today's ever-accelerating pace; and that speed, flexibility, adaptability to change are among the greatest concerns of firms in the new economy. It also indicated that organizations that are able to deploy Knowledge Management (KM) to meet current and future performance capacity are those organizations that build the right kinds of change management capacity into their operating systems. The study conclude that superior change-management practices that build an organization's adaptive capacity by considering the individual employees, team, organization, and industry, will support sustainable high performance now and in the future.
Robust PID Design for Parameter Uncertain SOPTD Processes Based on Reference Model Approximation

To design robust PID controllers for second-order plus time delay (SOPTD) processes with parameter uncertainty, a reference model approximation method is proposed in this study. The central idea is to enable the frequency response of the PID controller to approximate that of a user-specified reference model. A convex hull is utilized to approximate the frequency template of the parameter uncertain process, and the maximum approximation error of the reference model among all candidate processes is bounded. To guarantee that the PID controller can well shape the closed-loop response of each candidate model to the reference model, a convex optimization problem is formulated to compute the PID parameters by minimizing the upper bound of the approximation error. Constraints on the closed-loop maximum sensitivity peak are imposed on the reference-model approximation problem for loop robustness. The proposed method is able to ensure balanced tracking performance and * Corresponding author at: Department of Automation, Tsinghua University, E-mail address: huangdx@tsinghua.edu. cn. 2 disturbance rejection performance through a proper specification of the reference model, and illustrative examples are presented to demonstrate the applicability.