Miracle-dz/UniFAR-FTUs huggingface dataset:
Trypanosoma cruz/‐induced decrease in the level of interferon‐7 receptor expression by resting and activated human blood lymphocytes

A substantial proportion of human peripheral blood mononuclear cells (PBMC) manifested a decreased capacity to express membrane interferon‐γ receptors (IFN‐γR) when co‐cultured with Trypanosoma cruzi. Among the lymphocytes, B cells accounted for the bulk of this effect, evidenced by a marked drop in the proportion of CD19+ or CD20+ cells expressing IFN‐γR. Decreased IFN‐γR expression by B lymphocytes was seen as early as 3 h after co‐culture with T. cruzi and persisted for at least 24 h. The parasite had no detectable effect on CD19, CD20 or DR antigen expression by B lymphocytes. Neither the proportion ofB cells expressing these markers nor the membrane density of these molecules varied significantly in the presence of T. cruzi. In PBMC cultures stimulated with Staphlyococcus aureus Cowan I (SACI), T. cruzi decreased the percentages of both IFN‐γR+ and IFN‐R +bnght (cells expressing above‐normal levels of surface IFN‐γR) B lymphocytes. Cell‐free filtrates of T. cruzi suspensions reproduced the suppressive effects of living parasites on IFN‐γR expression by B cells. When T. cruzi was present, the intracellular levels of IFN‐γR molecules in resting or SACI‐activated B lymphocytes, represented by fluorescence intensity, were well below control values, suggesting that decreased surface expression resulted from suppressed IFN‐γR synthesis. Among T (CD3+) cells, 10–8% to 39–6% (7 donors) expressed surface IFN‐γR and did so at a very low level. These percentages were also reduced by T. cruzi. If occurring in the host, downregulated expression of IFN‐γR could curtail the utilization of IFN‐γ, known to play a critical role in host defence against T. cruzi infection.
Evaluation of Matrix Issues in the Applicability of the Neuro-2a Cell Based Assay on the Detection of CTX in Fish Samples

Ciguatoxins (CTXs) are a group of neurotoxins responsible for the syndrome ciguatera fish poisoning (CFP) as a result of the consumption of contaminated fish. The presence of these toxins has been detected around the Pacific, Caribbean and Indian coasts. Recent reports indicate the emergence of CFP in other geographic areas, in particular in European coasts, of the Canary Islands (Spain) and Madeira (Portugal). A neuroblastoma cell line of murine origin (N2a) has been applied to assay different groups of neurotoxins, acting on voltage-gated sodium channel (VGSC) of excitable cells, N2a-MTT. The great potential of N2a-MTT as a sensitive tool for the CTXs screening is clearly recognized, notably because it allows the detection of these toxins at levels below recommended as security levels. However, the complexity of the matrix is a critical point on the application of N2a-MTT, which needs to be evaluated. The aim of this work is to provide recommendations for an implemented N2a-MTT method for CTXs determination in fish that avoids matrix effects, particularly those related to high lipid content.
The Area and Number of Intraretinal Cystoid Spaces Predict the Visual Outcome after Ranibizumab Monotherapy in Diabetic Macular Edema

Visual outcomes in diabetic macular edema (DME) after anti-vascular endothelial growth factor therapy vary across individuals. We retrospectively reviewed the clinical records for 46 treatment-naive eyes of 46 patients with DME who underwent intravitreal ranibizumab (IVR) monotherapy with a pro re nata regimen for 12 months. Overall, mean best-corrected visual acuity (BCVA) improved. Multivariate analyses adjusted for age and baseline BCVA showed that the area ratio, compared with the retinal area, and the number of intraretinal cystoid spaces evaluated on OCT (optical coherence tomography) images at baseline positively correlated with LogMAR BCVA and the extents of ellipsoid zone and external limiting membrane disruption at 12 months, and negatively correlated with central retinal thickness at the time of edema resolution. Therefore, a high area ratio and large number of intraretinal cystoid spaces resulted in a disorganized outer retinal structure at 12 months, a thin and atrophic retina after edema resolution, and a worse visual outcome. The area ratio and number of intraretinal cystoid spaces on initial OCT images were predictors of the visual outcome after IVR therapy in DME irrespective of baseline age and BCVA. The factors were related to retinal neurodegenerative changes in DME and could help in obtaining proper informed consent before treatment.
Dental prophylaxis influence in tooth color assessment-Clinical study.

OBJECTIVE To evaluate dental prophylaxis influence in tooth color assessment with the use of different methods. MATERIALS AND METHODS Volunteers were consecutively recruited and screened according to appropriate inclusion/exclusion criteria. Each participant's upper right central incisive and canine color was measured before and after dental prophylaxis with a one-week interval. Tooth color assessment was performed by calibrated operators and the patient using a VITA Classical shade guide and by spectrophotometric methods with a proper device (SpectroShade). Color was reported as mean with SD of Commission Internationale De l'Eclairage (CIE) L*a*b* values, ΔEab and ΔE00 . One-way analysis of variance and Tukey post hoc were performed to assess differences between methods and paired t test for assessing differences in tooth color coordinates after dental prophylaxis. Values of P < . 05 were taken as significant. RESULTS Fifty patients were included. Self-assessed dental prophylaxis effects equated to a significantly different mean ΔE00 value of 2.3 ± 1.7 (P < .01), when compared to the calibrated operator reported 0.8 ± 1.1 and the spectrophotometer 1.0 ± 0.5. Half of volunteer's ΔE00 values surpassed the acceptability threshold, when compared to 28% from investigator and 10% from spectrophotometer. CONCLUSION Performing a dental prophylaxis did have an influence in tooth color perception with a higher self-perceived effect in the patient assessment. CLINICAL SIGNIFICANCE The results of this study suggest that performing professional dental prophylaxis presented a perceived effect in tooth color regardless of the method used being that patients precepted whiter and less yellowish teeth. Since extrinsic stain is considered as one of the factors that could influence tooth color assessment, performing professional dental prophylaxis prior to composite or ceramic color selection in anterior teeth restorations could consequently increase treatment predictability.
Restriction fragment length polymorphisms in mitochondrial DNA and ribosomal RNA gene complexes as an aid to the characterization of species and sub-species populations in the genus Verticillium

Genomic DNA was extracted from seven species of Verticillium and digested with the restriction endonucleases EcoRI or HaeIII. Hybridization with an homologous V. albo-atrum ribosomal RNA gene probe revealed restriction fragment length polymorphisms (RFLPs) which could differentiate V. lateritium, V. lecanii, V. nigrescens, V. nubilum and V. tricorpus. Digestion with EcoRI did not provide RFLPs which could distinguish between V. albo-atrum and V. dahliae. Digestion of genomic and mitochondrial DNA with HaeIII showed distinctive patterns on ethidium bromide gels which allowed each species to be distinguished. Some intra-species variation in patterns occurred and a combination of mitochondrial and ribosomal RNA gene complex RFLPs has potential as an aid for the characterization of species and sub-species populations in the genes Verticillium.
Fossil Ericales from the Upper Cretaceous of New Jersey

Premise of research. Fossil flowers and other related organs of Turonian age from Cretaceous Atlantic Coastal Plain deposits in northern New Jersey are remarkable in their degree of preservation and document important events in the radiation of eudicot angiosperms relatively early in their history. Methodology. Fossils isolated from silty clays have been examined with light microscopy, TEM, SEM, and micro-CT scan to illuminate their structural details. Fossil characters were placed in data matrices that included morphological and molecular genetic characters to place them phylogenetically in order to test the hypothesis that they are extinct representatives of the modern Ericales. Pivotal results. These fossils represent a clade of three species and include flowers, inflorescences, and leaves, constituting a genus that is unique relative to existing angiosperm genera but that nonetheless shares a significant number of characters with living families placed in the broader Ericales (APG III) including Ericaceae. Fossils representing this taxon include dispersed individual flowers, inflorescences, fruits, and leaves. Conclusions. These fossils are interpreted as representing a complex of at least three closely related ericalean species because of common morphological characters, including those shared by the flowers and fruits. These include five-merous, haplostemonous, hypogynous flowers; staminodes opposite the clawed petals; distinctive sessile abaxial sepal glands; papillate ovaries; five-lobed capitate stigmas; and numerous reticulate seeds in valvate loculicidal capsules. Variation among the three species as delimited here includes trichome form and distribution. Based on morphological cladistic analyses, the fossils are placed within Ericales near modern Clethraceae and Cyrillaceae, outside of but close to the clade of extant (core) Ericaceae. The flower fossils have some interesting characteristics suggestive of well-developed relationships with pollinating insects, including, in one of the species, pollen grains united by fine threads (interpreted as viscin threads) and anther spurs often associated with hymenopteran pollination in modern taxa.
Homocysteine induces melanocytes apoptosis via PERK-eIF2α-CHOP pathway in vitiligo.

Vitiligo is a depigmentation disorder that develops as a result of the progressive disappearance of epidermal melanocytes. The elevated level of amino acid metabolite homocysteine (Hcy) has been identified as circulating marker of oxidative stress and known as a risk factor for vitiligo. However, the mechanism underlying Hcy-regulated melanocytic destruction is currently unknown. This study aims to elucidate the effect of Hcy on melanocytic destruction and its involvement in the pathogenesis of vitiligo. Our results showed that Hcy level was significantly elevated in the serum of progressive vitiligo patients. Notably, Hcy induced cell apoptosis in melanocytes via activating reactive oxygen species (ROS) and endoplasmic reticulum stress protein kinase RNA-like ER kinase (PERK)-eukaryotic translation initiation factor 2α (eIF2α)-C/EBP homologous protein (CHOP) pathway. More importantly, folic acid, functioning in the transformation of Hcy, could lower the intracellular Hcy level and further reverse the apoptotic effect of Hcy on melanocytes. Additionally, Hcy disrupted melanogenesis whereas folic acid supplementation could reverse the melanogenesis defect induced by Hcy in melanocytes. Taken together, Hcy is highly increased in vitiligo patients at progressive stage, and our in vitro studies revealed that folic acid could protect melanocytes from Hcy-induced apoptosis and melanin synthesis inhibition, indicating folic acid as a potential benefit agent for patients with progressive vitiligo.
Neural Responses to Infant Emotions and Emotional Self-Awareness in Mothers and Fathers during Pregnancy

Neuroscientific research has largely investigated the neurobiological correlates of maternal and (to a much lesser extent) paternal responsiveness in the post-partum period. In contrast, much less is known about the neural processing of infant emotions during pregnancy. Twenty mothers and 19 fathers were recruited independently during the third trimester of pregnancy. High-density electroencephalography (hdEEG) was recorded while expectant parents passively viewed images representing distressed, ambiguous, happy, and neutral faces of unknown infants. Correlational analyses were performed to detect a link between neural responses to infant facial expressions and emotional self-awareness. In response to infant emotions, mothers and fathers showed similar cerebral activity in regions involved in high-order socio-affective processes. Mothers and fathers also showed different brain activity in premotor regions implicated in high-order motor control, in occipital regions involved in visuo-spatial information processing and visual mental imagery, as well as in inferior parietal regions involved in attention allocation. Low emotional self-awareness negatively correlated with activity in parietal regions subserving empathy in mothers, while it positively correlated with activity in temporal and occipital areas implicated in mentalizing and visual mental imagery in fathers. This study may enlarge knowledge on the neural response to infant emotions during pregnancy.
Discordant Dose-Dependent Metabolic Effects of Eicosapentanoic Acid in Diet-Induced Obese Mice

Obesity is a widespread epidemic that increases the risk for several metabolic diseases. Despite several beneficial health effects of eicosapentaenoic acid (C20:5n-3, EPA), previous studies have used very high doses of EPA. In this study, dose-dependent effects of EPA on metabolic outcomes were determined in diet-induced obese mice. We used B6 male mice, fed high-fat diet (HF, 45% kcal fat) or HF diet supplemented with 9, 18, and 36 g/kg of EPA-enriched fish oil for 14 weeks. We conducted metabolic phenotyping during the feeding period, and harvested tissues and blood at termination. Only mice fed 36 g/kg of EPA significantly (p < 0.05) lowered body weight, fat content and epididymal fat pad weight, compared to HF. Both 18 and 36 g/kg doses of EPA significantly increased glucose clearance and insulin sensitivity, compared to HF or 9 g/kg of EPA. Locomotor activity was significantly increased with both 18 and 36 g/kg doses of EPA. Interestingly, all doses of EPA compared to HF, significantly increased energy expenditure and oxygen consumption and significantly reduced serum insulin, leptin, and triglycerides levels. These results demonstrate weight- and adiposity-independent metabolic benefits of EPA, at doses comparable to those currently used to treat hypertriglyceridemia.
Liver transplant for neurologic Wilson disease

Wilson disease (WD) is a monogenic, autosomal recessively inherited copper storage disorder due to biallelic mutations in the copper-transporting protein ATP7B. The 2 most common presentations are the hepatic presentation (typically in childhood) and the neurologic presentation (typically in early adulthood). 1 However, later onset, especially for patients with the neurologic presentation, is well recognized, with some patients presenting in their 70s.2 Recent genetic studies suggest that the previously accepted and widely published prevalence figure of 1:30,000 may be an underestimate, but this is still a matter of debate.3,4 The observation of Kayser-Fleischer (KFR) rings can quickly establish the clinical diagnosis in patients with the neurology textbook presentation of wing-beating tremor combined with dysarthria. However, many other patients will present with a more complex movement disorder.5
Anti-Inflammatory Strategies Targeting Metaflammation in Type 2 Diabetes

One of the concepts explaining the coincidence of obesity and type 2 diabetes (T2D) is the metaflammation theory. This chronic, low-grade inflammatory state originating from metabolic cells in response to excess nutrients, contributes to the development of T2D by increasing insulin resistance in peripheral tissues (mainly in the liver, muscles, and adipose tissue) and by targeting pancreatic islets and in this way impairing insulin secretion. Given the role of this not related to infection inflammation in the development of both: insulin resistance and insulitis, anti-inflammatory strategies could be helpful not only to control T2D symptoms but also to treat its causes. This review presents current concepts regarding the role of metaflammation in the development of T2D in obese individuals as well as data concerning possible application of different anti-inflammatory strategies (including lifestyle interventions, the extra-glycemic potential of classical antidiabetic compounds, nonsteroidal anti-inflammatory drugs, immunomodulatory therapies, and bariatric surgery) in the management of T2D.
Reconfiguring Nature’s Cholesterol Accepting Lipoproteins as Nanoparticle Platforms for Transport and Delivery of Therapeutic and Imaging Agents

Apolipoproteins are critical structural and functional components of lipoproteins, which are large supramolecular assemblies composed predominantly of lipids and proteins, and other biomolecules such as nucleic acids. A signature feature of apolipoproteins is the preponderance of amphipathic α-helical motifs that dictate their ability to make extensive non-covalent inter- or intra-molecular helix–helix interactions in lipid-free states or helix–lipid interactions with hydrophobic biomolecules in lipid-associated states. This review focuses on the latter ability of apolipoproteins, which has been capitalized on to reconstitute synthetic nanoscale binary/ternary lipoprotein complexes composed of apolipoproteins/peptides and lipids that mimic native high-density lipoproteins (HDLs) with the goal to transport drugs. It traces the historical development of our understanding of these nanostructures and how the cholesterol accepting property of HDL has been reconfigured to develop them as drug-loading platforms. The review provides the structural perspective of these platforms with different types of apolipoproteins and an overview of their synthesis. It also examines the cargo that have been loaded into the core for therapeutic and imaging purposes. Finally, it lays out the merits and challenges associated with apolipoprotein-based nanostructures with a future perspective calling for a need to develop “zip-code”-based delivery for therapeutic and diagnostic applications.
The structures of cationic gold clusters probed by far-infrared spectroscopy.

Determining the precise structures of small gold clusters is an essential step towards understanding their chemical and physical properties. Due to the relativistic nature of gold, its clusters remain planar (2D) up to appreciable sizes. Ion mobility experiments have suggested that positively charged gold clusters adopt three-dimensional (3D) structures from n = 8 onward. Computations predict, depending on the level of theory, 2D or 3D structures as putative energy-minimum for n = 8. In this work, far-infrared multiple photon dissociation spectroscopy, using Ar as tagging element, is combined with density-functional theory calculations to determine the structures of Aun+ (n ≤ 9) clusters formed by laser ablation. While the Au frameworks in Au6Arm+ and Au7Arm+ complexes are confirmed to be planar and that in Au9Arm+ three-dimensional, we demonstrate the coexistence of 3D and planar Au8Arm+ (m = 1-3) isomers. Thus, it is revealed that at finite temperatures, the formal 2D to 3D transition takes place at n = 8 but is not sharp.
Mode II Interfacial Fracture Toughness of Multi-Walled Carbon Nanotubes Reinforced Nanocomposite Film on Aluminum Substrate

In this investigation, various loadings of multi-walled carbon nanotubes (MWCNTs) ranging from 0.3–1.0 wt % were incorporated into the epoxy to fabricate the nanocomposites. Nanocomposite film with a thickness of 0.2 mm was deposited on an aluminum substrate through a hot-pressing process. Theoretical expression of the model II strain energy release rate for the film/substrate composite structure was derived. End-notched flexure (ENF) tests were performed to characterize the mode II fracture energy of the composite structure. Experimental results indicate that the elastic modulus, ultimate strength, and mode II fracture energy increase as the MWCNT loading in the nanocomposite increases. In the case of nanocomposite film with 1.0 wt % of MWCNTs, the elastic modulus, ultimate strength, and mode II interfacial fracture toughness are increased by 20.6%, 21.1%, and 54.4%, respectively in comparison with neat epoxy. In addition, the dispersion of MWCNTs in the epoxy-based matrix was investigated using scanning electron microscope (SEM). The SEM images depict that MWCNTs are well dispersed leading to the enhancement of the mechanical properties of the nanocomposite.
Exercise Strategies to Prevent Hypoglycemia in Patients with Diabetes

The importance of adopting healthy exercise routines has been repeatedly emphasized to individuals with diabetes mellitus (DM). However, knowledge about the risk of exercise-induced hypoglycemia is limited. Regular exercise reduces and delays the onset of DM-related complications particularly in individuals who already have DM. However, an excessive exercise can lead to hypoglycemia. Excessive exercise in the evening can cause hypoglycemia while sleeping. Furthermore, if individuals with DM want to have a greater amount of exercise, the exercise duration rather than intensity must be increased. In weight resistance exercises, it is beneficial to first increase the number of repetitions, followed by the number of sets and gradually the weight of resistance. When performing intermittent high-intensity training within a short time period, hypoglycemia may develop for an extended period after exercise. In addition to adjusting exercise regimens, the medication doses must be modified accordingly. Delaying exercise, adjusting the number of snacks consumed prior to exercise, reducing insulin dose before exercise, and injecting insulin into the abdomen rather than the limbs prevent exercise-induced hypoglycemia prior to a spontaneous exercise. Ultimately, with personal knowledge on how to prevent hypoglycemia, the effects of exercise can be maximized in individuals with DM, and a healthy lifestyle can prevent future complications.
Erbin Enhances Voltage-Dependent Facilitation of Cav1.3 Ca2+ Channels through Relief of an Autoinhibitory Domain in the Cav1.3 α1 Subunit

Cav1.3 (L-type) voltage-gated Ca2+ channels have emerged as key players controlling Ca2+ signals at excitatory synapses. Compared with the more widely expressed Cav1.2 L-type channel, relatively little is known about the mechanisms that regulate Cav1.3 channels. Here, we describe a new role for the PSD-95 (postsynaptic density-95)/Discs large/ZO-1 (zona occludens-1) (PDZ) domain-containing protein, erbin, in directly potentiating Cav1.3. Erbin specifically forms a complex with Cav1.3, but not Cav1.2, in transfected cells. The significance of erbin/Cav1.3 interactions is supported by colocalization in somatodendritic domains of cortical neurons in culture and coimmunoprecipitation from rat brain lysates. In electrophysiological recordings, erbin augments facilitation of Cav1.3 currents by a conditioning prepulse, a process known as voltage-dependent facilitation (VDF). This effect requires a direct interaction of the erbin PDZ domain with a PDZ recognition site in the C-terminal domain (CT) of the long variant of the Cav1.3 α1 subunit (α11.3). Compared with Cav1.3, the Cav1.3b splice variant, which lacks a large fraction of the α11. 3 CT, shows robust VDF that is not further affected by erbin. When coexpressed as an independent entity with Cav1.3b or Cav1.3 plus erbin, the α11. 3 CT strongly suppresses VDF, signifying an autoinhibitory function of this part of the channel. These modulatory effects of erbin, but not α11. 3 CT, depend on the identity of the auxiliary Ca2+ channel β subunit. Our findings reveal a novel mechanism by which PDZ interactions and alternative splicing of α11. 3 may influence activity-dependent regulation of Cav1.3 channels at the synapse.