Miracle-dz/UniFAR-FTUs huggingface dataset:
Towards the development of standardized potency assays in regenerative medicine bioprocessing for the treatment of ischemic cardiac injury

The mechanisms by which mesenchymal cells can repair the infarcted myocardium are still unclear and are made more challenging by the fact that hMSCs engraft in the myocardium for only a short amount of time. This thesis first examined the ability of commercial bone marrow (BM) MSCs expanded in low serum (<5% FBS medium), umbilical cord (UC) MSCs and individual donor BM-MSCs to perform biologically relevant functions. The characterization studies showed that the differently-sourced hMSCs successfully underwent tri-lineage differentiation and displayed similar expression levels of positive MSC markers. Additionally to this, there was a tendency for cells at increased in vitro age to display reduced expression of CD105. Biologic priming of cells using sJag1 typically enhanced MSC attachment to fibronectin, although to varying degrees in the different MSC types. In addition, vascular support assays revealed that MSCs displayed pericyte-like behaviour lining the outside of the vessels and bridging in between endothelial cells during network formation. Assessment of how bioprocess parameters affect vascular tubule formation revealed that economic benefits can be derived by using lower volumes of alternative matrix substrates. In addition, automated counting tools achieved an unbiased measurement compared to manual counting processes. Finally, from a perspective of the vascular endothelial cells used in the assays, it was possible to extend their use an additional 50%, from passage 10 to passage 15 before losing functional capacity. With further work, these assays could be optimized for high-throughput screening and be used in industry as surrogate tests for quality control (QC) hence enabling the advancement of well-characterized cell therapy products.
OPTIMAL BENCH MARK DISTRIBUTION TO MONITOR SOUTHERN SEGMENT OF SUMATRAN FAULT

<p><em>The s</em><em>outhern segment of</em><em> a</em><em> Sumatran fault zone </em><em>was</em><em> one of </em><em>the </em><em>sources of earthquakes in Lampung Province. The source of hazard c</em><em>ame</em><em> from stress accumulation of crust</em><em>,</em><em> which can be derived from movement of </em><em>bench mark</em><em> in surface. </em><em> Lack of research in southern segment </em><em>was</em><em> caused by small numbers of monitoring bench mark. </em><em>This research show</em><em>n</em><em> optimal monitoring bench mark distribution by considering existing bench mark and location which is decent and representative to monitor Sumatran Fault Zone movement by considering on its position relative to Sumatran Fault Zone, earthquake history, Digital Elevation Model and land use. </em> <em>Decent location </em><em>was</em><em> determined by overlaying land use and slope processed from Digital Elevation Model</em><em>. </em> <em>Representative location was determined by taking into account the distance to Sumatran Fault Zone and earthquake history. </em> <em>Very decent location is around 10.5 percent of the total area. Very representative location is around 44.5 percent of the total area. </em> <em>There are total 15 planned bench marks location to make southern Segment of Sumatran Fault Zone monitoring bench mark more optimal. </em><em></em></p>
Care Team Utilization of the GINA Guidelines to Improve Quality of Pediatric Asthma Care

Background: Asthma is a chronic condition that affects millions of people, many of whom are children. There is a significant amount of evidence detailing best step-by-step treatments based on severity, yet many children still have uncontrolled asthma. The comprehensive management of asthma is essential to improve health outcomes for children with asthma. Methods: This project evaluated the implementation of the Global Initiative for Asthma (GINA) guidelines and assessed the barriers and facilitators present in a pediatric clinic which impact care delivery. This project involved the implementation of an educational intervention based on the GINA guidelines to facilitate a team-based approach to asthma management. Results: Eight staff members participated in the project. The most significant changes were 75% of staff reported an increase in their knowledge of the GINA guideline recommendations, staff reported a 25% increase in team member participation in asthma care, and staff reported a 12.5% increase in their feelings of support for the use of team-based implementation of the GINA guidelines from leadership. The results of this quality improvement project support the use of Roger’s Diffusion of Innovations model as a theoretical framework to support implementation of a team-based approach to the use of the GINA guidelines in a pediatric clinic. Conclusions: Education on evidence-based guidelines improves clinic staff knowledge and utilization of the guidelines. However, factors such as leadership involvement, clinic infrastructure, and standardized clinical tools are essential for successful implementation of a team-based approach to asthma management.
The European Network of Centres for Pharmacoepidemiology and Pharmacovigilance (ENCePP). Guide on Methodological Standards in Pharmacoepidemiology (Revision 1, 2012, Revision 2, 2013, Revision 3, 2014)).

The ENCePP initiative aims to strengthen post-authorisation medicines research by facilitating the conduct of multi-centre, independent and scientifically robust studies focusing on safety and the balance of benefits and risks. This includes supporting high quality research and stimulating innovation that benefits patients and public health at large. The Guide on Methodological Standards in Pharmacoepidemiology has, therefore, been developed to offer a single overview document and web resource for methodological guidance for both experienced and new researchers in pharmacoepidemiology and pharmacovigilance. For each topic covered, direct electronic access is given to internationally agreed recommendations and key points from important guidelines, published articles and textbooks after an introductory review. The focus is on scientific rather than regulatory guidance although relevant legislation and good pharmacovigilance practice are cited, where appropriate. Where relevant, gaps in guidance in areas important to collaborative pharmacoepidemiology research have been addressed with what ENCePP considers good practice. The guide has been developed by a dedicated ENCePP Working Group consisting of experts in pharmacoepidemiological and pharmacovigilance research. The original guide was published online on 17 May 2011. Both the original and the current first revision of the guide have been subject to public consultation. The guide will be updated annually by structured review to maintain its dynamic nature. It may also be amended as necessary on an ad-hoc basis in response to comments received. Researchers are therefore kindly requested to refer any additional guidance document that they consider relevant, to encepp_comments@ema.europa. eu.
A clinico-radiological and pathological profile of lung cancer patients presented to All India Institute of Medical Sciences (Patna)

Introduction: Apoptosis or programmed cell death is triggered by Fas-Fas ligand (FasL) binding. Mutation in the active sites of these genes blocks death signal transmission and ultimately causes carcinogenesis. The present case–control study was conducted to compare the relationship of age and serum prostate-specific antigen (PSA) with FAS-1377 G/A (rs2234767) polymorphism as one of the best-known polymorphisms of FAS in patients with prostate cancer and a group of controls. Materials and Methods: A total of 100 cases diagnosed with prostate cancer by a specialist and 100 healthy controls were selected from those presenting to the Urogenital Research Unit of Imam Khomeini Hospital in Tehran, Iran. The individual's blood samples were taken, and the enzyme-linked immunosorbent assay method was then used to isolate their blood serum and measure its PSA levels. The saturated salt solution method was used to extract the leukocyte DNA, and the individual's genotype was determined using the Restriction fragment length polymorphism-polymerase chain reaction technique. The data collected were analyzed in IBM SPSS-23. Results: The genotype count found genotype AA in 42% of the cancer patients and 36% of the controls (P = 0.384, odds ratio [OR] = 1.28 confidence interval [CI] 95%: 0.728–2.274), genotype GG in 57% of the cancer patients and 64% of the controls (P = 0.311, OR = 0.746, CI 95%: 0.422–1.317), and genotype AG in a single patient (1%) (P = 0.316, OR: 0.990, CI 95%: 0.971–1.010). No significant relationships were observed between the two groups in terms of these genotypes. Age and serum PSA were found to have significant relationships with both the AA and GG genotypes. Discussion: Older age and elevated serum PSA levels were found to be significantly related to the mutant genotype AA in the cancer patients. Given the lack of significant relationships between the risk allele A and prostate cancer, conducting a study with a larger sample size in different Iranian ethnic groups seems necessary, as sample size can affect the results obtained on this polymorphism.
Scientific Opinion on the state of the science on pesticide risk assessment for amphibians and reptiles

Abstract Following a request from EFSA, the Panel on Plant Protection Products and their Residues developed an opinion on the science to support the potential development of a risk assessment scheme of plant protection products for amphibians and reptiles. The coverage of the risk to amphibians and reptiles by current risk assessments for other vertebrate groups was investigated. Available test methods and exposure models were reviewed with regard to their applicability to amphibians and reptiles. Proposals were made for specific protection goals aiming to protect important ecosystem services and taking into consideration the regulatory framework and existing protection goals for other vertebrates. Uncertainties, knowledge gaps and research needs were highlighted.
New Strategies for Myocardial Infarction Treatment

Myocardial infarction is due to lasting and serious myocardial ischemic that leads to myocardial necrosis and cardiac remodeling, and can consequently results in heart failure. Traditional treatment for myocardial infarction includes drug therapy, thrombolytic therapy, percutaneous coronary intervention (PCI), and coronary artery bypass graft (CABG) surgery. Although these treatments can to some extent relieve the symptom of myocardial ischemia, they fail to repair the necrotic heart tissues. Myocardial regeneration is undoubtedly the best solution to the clinical problems of myocardial infarction treatment. The review briefly illustrated the pros and cons of the traditional treatments of myocardial infarction, and focused on the application of new strategies for myocardial infarction treatment using myocardial regeneration.
Use of simulation in teaching of anesthesiology in a school of medicine

AIMS: To report the experience with teaching of anesthesiology to medical students in a practical and interactive way, using simulators in clinical scenarios of anesthesia with predetermined tasks.EXPERIENCE REPORT: In the Medical School of the Pontifical Catholic University of Rio Grande do Sul simulated clinical settings are used in general anesthesia and anesthetic blockage teaching. Complete equipment and drugs specific to each simulation are available. Students have direct performance in the simulator to perform in the presented situations. For each conduct, choice of drugs and doses required, filming are performed with the purpose of fixing and improve knowledge.CONCLUSIONS: Medical teaching of anesthesiology with the use of simulators tends to be increasingly used, since it inserts the student in simulated situations close to the reality of the anesthetic process, in an interactive and attractive way without the real risk. Clinical situations of anesthesia or complications can be repeated until the fixation of knowledge and skills, before facing a situation with real patients.
Informe evaluación de impacto AMTEC

Este informe presenta los resultados de la evaluacion de impacto cuantitativa y cualitativa del programa de Adopcion Masiva de Tecnologia (AMTEC) en la productividad de las fincas arroceras en Colombia. Este programa surge de la necesidad de implementar un modelo de transferencia para difundir entre los productores las mejores practicas derivadas de procesos investigativos, asi como de la experiencia de transferencia existente, con el objetivo de aumentar los rendimientos y reducir los costos de produccion del cultivo del arroz. AMTEC fue concebido y disenado por FEDEARROZ- Fondo Nacional del Arroz, desde 2012 en el contexto de un conjunto de factores que indicen sobre el sector arrocero, como son la variabilidad y el cambio climatico, y los Tratados de Libre Comercio, y especialmente el suscrito con los Estados Unidos que tiene un periodo de desgravacion a 19 anos (Diagrama 1). Este ultimo, en particular, implica grandes desafios para mejorar la competitividad de la produccion nacional en un periodo de tiempo que va siendo cada vez mas estrecho dado que ya ha transcurrido cerca de la mitad del periodo total de desgravacion.
Registered Nurses’ understanding of the nursing standard requirement to provide professional development to nursing students on clinical placements: The theory of Doing the Right Thing

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Abundant expression of maternal siRNAs is a conserved feature of seed development

Significance Gene expression is regulated not only by DNA sequence, but also chemical modifications such as methylation. DNA methylation in plants is induced at thousands of genomic loci by 24-nucleotide (nt) small interfering RNAs (siRNAs). We show that during seed development, however, over 90% of 24-nt siRNAs are produced from fewer than 200 loci; this inequality of expression is conserved among plants. Rarely, chromosomal position of these loci can be conserved despite sequence evolution. siRNAs from these loci are abundant in seed coat and endosperm but not in leaves. Only maternal siRNAs are detected from these loci in the endosperm, suggesting that these siRNAs might move from maternal seed coat into filial tissues to establish DNA methylation in the next generation. Small RNAs are abundant in plant reproductive tissues, especially 24-nucleotide (nt) small interfering RNAs (siRNAs). Most 24-nt siRNAs are dependent on RNA Pol IV and RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) and establish DNA methylation at thousands of genomic loci in a process called RNA-directed DNA methylation (RdDM). In Brassica rapa, RdDM is required in the maternal sporophyte for successful seed development. Here, we demonstrate that a small number of siRNA loci account for over 90% of siRNA expression during B. rapa seed development. These loci exhibit unique characteristics with regard to their copy number and association with genomic features, but they resemble canonical 24-nt siRNA loci in their dependence on RNA Pol IV/RDR2 and role in RdDM. These loci are expressed in ovules before fertilization and in the seed coat, embryo, and endosperm following fertilization. We observed a similar pattern of 24-nt siRNA expression in diverse angiosperms despite rapid sequence evolution at siren loci. In the endosperm, siren siRNAs show a marked maternal bias, and siren expression in maternal sporophytic tissues is required for siren siRNA accumulation. Together, these results demonstrate that seed development occurs under the influence of abundant maternal siRNAs that might be transported to, and function in, filial tissues.
Neoarchean arc‐back arc subduction system in the Indian Peninsula: Evidence from mafic magmatism in the Shimoga greenstone belt, western Dharwar Craton

The Neoarchean Shimoga greenstone belt (SGB) of western Dharwar Craton is dominated by ultramafic‐mafic‐felsic magmatic suite and sedimentary rocks. Here, we investigate the mineral chemistry and whole‐rock geochemistry of the Koppa–Herambapura mafic magmatic rocks (tholeiitic metagabbro and metabasalt) from the southern SGB in order to understand their petrogenesis and geodynamic implication. The geochemical features classify the Shimoga rocks as basaltic‐basaltic andesite with a sub‐alkaline tholeiitic signature. Both the rock types display uniform depletion in HFSE relative to LILE‐LREE, higher LILE/HFSE, and LREE/HFSE ratios (La/Nb = 1.30–8.07, Nb/Ta = 11.58–16.19, Zr/Sm = 10.24–29.77) and negative Nb–Ta, Zr–Hf, and Ti anomalies in multi‐element patterns, corresponding to magmatic arc rocks generated within intraoceanic subduction environment. Their Nb/Th (2–15), Zr/Sm (10–29), and Zr/Hf (44–233) ratios indicate a depleted to enriched mantle source, and the parental magma of the mafic rock suite was generated by low to medium degrees (~8–30%) of mantle melting at depths corresponding to spinel to garnet peridotite fields of arc‐back arc setting. Our field and analytical data suggest that the tholeiitic metagabbros were derived from depleted mantle source generated in an arc environment, whereas the metabasalts had a mantle source attributed to (a) development of juvenile back‐arc rift; (b) upwelling of MORB‐like mantle; and (c) flux‐induced melting in the compositional domain of spinel‐garnet peridotite. Based on these results, we conclude that the mafic rock suite from the southern SGB possibly marks one of the earliest Neoarchean arc‐back arc subduction in the western Dharwar Craton.
Circulating microRNAs: are they indicators for the prediction of radiotherapy-induced normal tissue injury

A large number of patients develop normal tissue injury following radiotherapy and this problem is becoming an important survivorship issue as the rate of curing cancer is increasing. Although there is no biomarker in clinical use for identification of patients that would develop adverse effects following radiotherapy, two proteins including TGF-β1 and IL-6 have been shown to be involved in radiation-induced normal tissue toxicity and have been used as biomarkers for the prediction of radiotherapy-induced adverse effects in several independent studies. Different expressions of these proteins may partly explain the individual differences in radiotherapy toxicity observed in patients.
The distribution pattern and feeding behaviour of malaria vectors in two selected communities of Karu Local Government Area of Nasarawa State

: An entomological survey was conducted to determine the malaria vectors density and their distribution. The study was aimed at identifying the distribution and feeding behaviour of major malaria vectors. Mosquitoes were sampled using Pyrethroid Spray Collection (PSC) and CDC light trap methods to collect indoors and outdoors resting mosquitoes for a period of three months (April, May and June) and were identified morphologically. A total of 462(100%) mosquitoes were collected in the study areas, 238(51.5%) were collected in Gitata and 224(48.5%) were collected in Panda. Of the 462 (100%) mosquitoes collected, 433(93.7%) were Anopheline while 29(6.3%) were Culicine. Statistically, there was a statistical difference (p<0.05) in the distribution of malaria vectors encountered in the study areas. Out of the 433(100%) Anopheles mosquitoes collected, 279(64.4%) feed indoor while 154(35.6%) feed outdoor. The indoor resting density of the mosquitoes were also calculated, the result showed that Panda had the highest indoor resting density of 7.5 than Gitata 7.0. The human biting rate (HBR) of mosquitoes collected in each month at the study areas were also determine and the result showed a high biting rate of 2.515 in Panda compared to 2.321 in Gitata. The parity rate (PR) of the mosquitoes collected in Gitata and Panda for the three months were also analyzed, and the result showed that, out of the 433 Anopheles mosquitoes collected, 146(100%) were dissected, 85(58.2%) were parous and 61(41.8%) were nulliparous. This study therefore, will be used as a baseline data to help in designing strategies for the control of malaria vector diseases in Karu Local Government of Nasarawa State and its environs.
Numerical Study on Hydrodynamic Responses of Floating Rope Enclosure in Waves and Currents

Enclosure aquaculture is a healthy and ecological aquaculture pattern developed in recent years to relieve the pressure due to the wild fish stock decline and water pollution. The object of this paper was a floating rope enclosure, which mainly consisted of floaters, mooring lines, sinkers and a net. In order to optimize mooring design factors, the hydrodynamic responses of the floating rope enclosure with different mooring systems in combined wave-current were investigated by experimental and numerical methods. Physical model experiments with a model scale of 1:50 were performed to investigate the hydrodynamic characteristics of a floating rope enclosure with 12 mooring lines. Based on the lumped mass method, the numerical model was established to investigate the effects of mooring design factors on the mooring line tension, force acting on the bottom, and the volume retention of the floating rope enclosure. Through the analysis of numerical and experimental results, it was found that the maximum mooring line tension of the floating rope enclosure occurs on both sides of the windward. Increasing the number of mooring lines on the windward side is helpful to reduce the maximum mooring line tension. Waves and current both have an influence on the mooring line tension; in contrast, currents have a more obvious effect on the mooring line tension than waves. However, the influence of the wave period on the maximum mooring line tension is small. The force endured by the bottom of the floating rope enclosure also changes periodically with the wave period. Yet, the maximum force endured by the bottom of floating rope enclosure occurred at the windward and leeward of the structure. The volume retention of the floating rope enclosure increased with the increasing amount of mooring lines.