Miracle-dz/UniFAR-FTUs huggingface dataset:
The impact of the metal interface on the stability and quality of a therapeutic fusion protein.

Subvisible particle formation which occurs after the sterile filtration step of the fill/finish process is challenge that may occur during the development of biotherapeutics with complex molecular structures. Here, we show that a stainless-steel pump head from a rotary piston pump produces more protein aggregates, past the limit of the acceptable quality range for sub-visible particle counts, in comparison to a ceramic pump head. Quartz-crystal microbalance was used to quantify the primary layer, a protein irreversibly adsorbed at the solid-liquid interface, and the secondary diffuse gel like layer interacting on top of the primary layer. The results showed the mass of irreversibly protein adsorbed onto stainless steel sensors is greater than on an aluminum oxide surface (ceramic pump mimic). This suggests that the amount of adsorbed protein plays a role on surface-induced protein aggregation at the solid-liquid interface.
A Systematic Review of Network Meta-Analyses and Real-World Evidence Comparing Apixaban and Rivaroxaban in Nonvalvular Atrial Fibrillation

There is no direct evidence comparing the 2 most commonly prescribed direct oral anticoagulants, apixaban and rivaroxaban, used for stroke prevention in nonvalvular atrial fibrillation (NVAF). A number of network meta-analyses (NMAs) of randomized control trials and real-world evidence (RWE) studies comparing the efficacy, effectiveness, and safety of apixaban and rivaroxaban have been published; however, a comprehensive evidence review across the available body of evidence is lacking. In this study, we aimed to systematically review and evaluate the clinical outcomes of apixaban and rivaroxaban using a combination of data gleaned from both NMAs and RWE studies. The review identified 21 NMAs and 5 RWE studies. The data demonstrated that apixaban was associated with fewer major bleeding events compared to rivaroxaban. There was no difference in the efficacy/effectiveness profiles between these treatments. Bleeding is a serious complication of anticoagulation therapy for the management of NVAF, and is associated with increased rates of hospitalization, morbidity, mortality, and health-care expenditure. The majority of studies in this comprehensive evidence review suggests that apixaban has a lower risk of major bleeding events compared to rivaroxaban in patients with NVAF.
ATTENUATION OUTER RETINAL BANDS ON OPTICAL COHERENCE TOMOGRAPHY FOLLOWING MACULAR EDEMA: A Possible Manifestation of Photoreceptor Misalignment.

PURPOSE Macular edema is a common retinal disease which may leave important anatomical and functional sequelaes. Directional fundus imaging consists of comparing on- and off-axis images to reveal angle-dependent reflectance properties of fundus structures, which may be related to misaligned photoreceptors. Here, we analyzed directional optical coherence tomography (OCT) and flood-illumination adaptive optics ophthalmoscopy images to detect evidence of misaligned photoreceptors following macular edema. METHODS Transversal, observational study. Nine patients having recovered a normal macular profile after macular edema due to retinal vein occlusion were included. For each patient, a reference OCT scan (i.e., with the incident beam normal to the fovea) was acquired, and off-axis scans were then acquired by laterally shifting the entry pupil. In addition, in four of these eyes, directional adaptive optics ophthalmoscopy documented the directional variations of cone metrics. RESULTS Included patients comprised two women and seven men (age range, 19-76 years). Reference OCT scans showed patchy attenuation of the cone outer segment tips and to a lesser extent of the inner segment/outer segment lines in all, but two eyes; these. Increased intensity of the cone outer segment tips and inner segment/outer segment lines could be observed on off-axis scans. Accordingly, fusion images showed 66% average reduction of the length of cone outer segment tips attenuation. In two cases, although reference scans showed continuity of outer bands, focal attenuation was evidenced in off-axis images. Directional adaptive optics ophthalmoscopy imaging showed a strong directional variability of cone counts in these areas, ranging from near absence to roughly two-third of reference values. In each case, directional variations of cone counts paralleled those of the reflectance of outer bands. CONCLUSION After macular edema, focal attenuations of the inner segment/outer segment and of the cone outer segment tips lines may be present on OCT. These areas may show a strong directional variability by both OCT and adaptive optics ophthalmoscopy, suggesting that misaligned photoreceptor outer segments contribute to such features. The evaluation of outer retinal damage following macular edema should therefore take into account the optical Stiles-Crawford effect to disambiguate missing from misaligned cones.
Prominence in French Dual Focus

This paper investigates how French signals prominence in prosody in the post-verbal domain of sentences with two objects or two adjuncts that vary in information status and prosodic length. The information status of particular interest here is dual focus, defined as the presence of two foci in a mono-clausal sentence, but other information states are investigated as well. The controlled production experiment we report on allows for a detailed examination of prosodic prominence. High boundary tones at the end of non-final prosodic phrases are pervasive, as has been documented in many studies before the present one. An important but less documented result is the variation in different prosodic curs, in particular in the number and position of high tones, as well as the particular scaling relationship between them, providing a powerful tool for the expression of (dual) focus. We also report on a perception experiment with our data, showing a clear tendency for French listeners to select the intended context question, recognizing dual focus better than other information states. Overall, this article provides elements of answers as to why French prosody is so difficult to pin down, and why contradictory results and analyses have been proposed for this language.
Root‐tip cutting and uniconazole treatment improve the colonization rate of Tuber indicum on Pinus armandii seedlings in the greenhouse

The Chinese black truffle Tuber indicum is commercially valuable. The main factors influencing the success or failure of a truffle crop include the mycorrhizal colonization rate and host plant quality. The effects of a plant growth regulator (uniconazole) and plant growth management technique (root‐tip cutting) on T. indicum colonization rate and Pinus armandii seedling growth were assessed under greenhouse conditions. The results indicated that 10 mg l−1 uniconazole or the combination of 5 mg l−1 uniconazole and root‐tip cutting constitutes an effective method for ectomycorrhizal synthesis based on an overall evaluation of colonization rate, plant biomass, plant height, root weight, stem circumference and antioxidant enzyme activities (SOD and POD) of P. armandii. The abundance of Proteobacteria in the rhizosphere of colonized seedlings might serve as an indicator of stable mycorrhizal colonization. This research inspires the potential application of uniconazole and root‐tip cutting treatments for mycorrhizal synthesis and truffle cultivation.
Reliable estimation of brain intravoxel incoherent motion parameters using denoised diffusion‐weighted MRI

In this study, we evaluate whether diffusion‐weighted magnetic resonance imaging (DW‐MRI) data after denoising can provide a reliable estimation of brain intravoxel incoherent motion (IVIM) perfusion parameters. Brain DW‐MRI was performed in five healthy volunteers on a 3 T clinical scanner with 12 different b‐values ranging from 0 to 1000 s/mm2. DW‐MRI data denoised using the proposed method were fitted with a biexponential model to extract perfusion fraction (PF), diffusion coefficient (D) and pseudo‐diffusion coefficient (D*). To further evaluate the accuracy and precision of parameter estimation, IVIM parametric images obtained from one volunteer were used to resimulate the DW‐MRI data using the biexponential model with the same b‐values. Rician noise was added to generate DW‐MRI data with various signal‐to‐noise ratio (SNR) levels. The experimental results showed that the denoised DW‐MRI data yielded precise estimates for all IVIM parameters. We also found that IVIM parameters were significantly different between gray matter and white matter (P < 0.05), except for D* (P = 0.6). Our simulation results show that the proposed image denoising method displays good performance in estimating IVIM parameters (both bias and coefficient of variation were <12% for PF, D and D*) in the presence of different levels of simulated Rician noise (SNRb=0 = 20‐40). Simulations and experiments show that brain DW‐MRI data after denoising can provide a reliable estimation of IVIM parameters.
ACTN3 R577X Genotype in Professional and Amateur Tennis Players.

Moreno-Pérez, V, Machar, R, Sanz-Rivas, D, and Del Coso, J. ACTN3 R577X genotype in professional and amateur tennis players. J Strength Cond Res XX(X): 000-000, 2019-Several investigations have concluded that the RR genotype in the ACTN3 R577X polymorphism is overrepresented in elite athletes of strength- and power-based sports when compared with nonelite populations, suggesting a positive role of this genotype on physical performance. However, no investigation has been geared to determine the distribution of this polymorphism in elite tennis players. The aim of this study was to compare the frequency distribution of the ACTN3 R577X genotype in professional and nonprofessional tennis players to determine whether this polymorphism has an association with tennis performance. A total of 128 tennis players volunteered to participate in this study. From the total, 56 were professional tennis players (16 were top 10 in Women Tennis Association/Association of Tennis Professionals [WTA/ATP] rankings, 22 were top 100 in WTA/ATP rankings, and 18 were ranked > top 100 in the WTA/ATP rankings), and the remaining 72 were categorized as nonprofessional. Chi-squared tests were used to assess differences in the distribution of RR, RX, and XX genotypes between the different performance categories. The distribution of the RR, RX, and XX genotypes was similar in professional (33.9, 48.2, and 17.9%) and nonprofessional tennis players (37.5, 40.3, and 22.2%; p = 0.650). Furthermore, the distribution of the ACTN3 R577X polymorphism was not different in top 10 (25.0, 50.0, and 25.0%), top 100 (31.8, 50.0, and 18.2%), and WTA/ATP-ranked players (44.4, 44.4, and 11.2%; p = 0.847). These results indicate that the distribution of the ACTN3 R577X genotype is similar in tennis players of very different performance levels. This outcome suggests that the ACTN3 genotype was not associated with elite tennis performance.
Draft Genome Sequence of Bacillus thuringiensis Strain UNMSM10RA, Isolated from Potato Crop Soil in Peru

The 5.5-Mb genome sequence of Bacillus thuringiensis strain UNMSM10RA, isolated from potato crop soil, is reported in this study. The strain UNMSM10RA contains 5,347 protein-coding sequences, 105 tRNA genes, 15 rRNA genes, and 5 noncoding RNA (ncRNA) genes, with an average G+C content of 35.1%. ABSTRACT The 5.5-Mb genome sequence of Bacillus thuringiensis strain UNMSM10RA, isolated from potato crop soil, is reported in this study. The strain UNMSM10RA contains 5,347 protein-coding sequences, 105 tRNA genes, 15 rRNA genes, and 5 noncoding RNA (ncRNA) genes, with an average G+C content of 35.1%.
Associations Between Sexual Violence and Women’s Sexual Attitudes, Sexual Self-Consciousness, and Sexual Self-Efficacy

Sexual violence, a public health problem, can be seen in all societies and among individuals from all levels of educational background. It is important to be aware of the social and individual factors associated with sexual violence to prevent and fight sexual violence. In this study, it was aimed to determine the associations between sexual violence and women’s sexual attitudes, sexual self-consciousness, and sexual self-efficacy. This is a descriptive study. The data were collected from 469 women admitted to three family health centers in Turkey between May and September 2018. Data were collected using a data collection form and three scales (the Sexual Self-Consciousness Scale, the Sexual Self-efficacy Scale, and the Hendrick Brief Sexual Attitudes Scale). The data were analyzed using descriptive statistics as well as the independent-sample t test and logistic regression analysis. It was found that 37.7% of the women were exposed to sexual violence. The most frequent response of the women against sexual violence was “crying” with 61%. The first reason why the women maintained the marriage despite sexual violence was to avoid their children growing up without a father (43.3%). There was a statistically significant difference between sexual violence and sexual embarrassment, sexual self-focus, sexual self-consciousness, and sexual self-efficacy scores of the women (p < .001). No significant difference between the means of the women’s sexual attitude scores and their sexual violence experiences was observed (p = .526). There was an association between sexual violence and sexual self-efficacy (p = .036; odds ratio [OR] = 1.030, 95% confidence interval [CI] = [1.002, 1.059]). Health professionals may provide sexuality education programs at family health centers, especially for couples preparing for marriage to increase sexual efficacy. Moreover, education may be provided identifying sexual violence acts in marriage and informative awareness programs may be offered regarding sexual violence.
Deficiency syndromes in top predators associated with large-scale changes in the Baltic Sea ecosystem

Vitamin B1 (thiamin) deficiency is an issue periodically affecting a wide range of taxa worldwide. In aquatic pelagic systems, thiamin is mainly produced by bacteria and phytoplankton and is transferred to fish and birds via zooplankton, but there is no general consensus on when or why this transfer is disrupted. We focus on the occurrence in salmon (Salmo salar) of a thiamin deficiency syndrome (M74), the incidence of which is highly correlated among populations derived from different spawning rivers. Here, we show that M74 in salmon is associated with certain large-scale abiotic changes in the main common feeding area of salmon in the southern Baltic Sea. Years with high M74 incidence were characterized by stagnant periods with relatively low salinity and phosphate and silicate concentrations but high total nitrogen. Consequently, there were major changes in phytoplankton and zooplankton, with, e.g., increased abundances of Cryptophyceae, Dinophyceae, Diatomophyceae and Euglenophyceae and Acartia spp. during high M74 incidence years. The prey fish communities also had increased stocks of both herring and sprat in these years. Overall, this suggests important changes in the entire food web structure and nutritional pathways in the common feeding period during high M74 incidence years. Previous research has emphasized the importance of the abundance of planktivorous fish for the occurrence of M74. By using this 27-year time series, we expand this analysis to the entire ecosystem and discuss potential mechanisms inducing thiamin deficiency in salmon.
Rituximab in refractory chronic inflammatory demyelinating polyneuropathy

Chronic inflammatory demyelinating polyneuropathy (CIDP) is a disorder in which early effective treatment is important to minimize disability from axonal degeneration. It has been suggested that some patients with CIDP may benefit from rituximab therapy, but there is no definitive evidence for this.
The effect of psyllium consumption on weight, body mass index, lipid profile, and glucose metabolism in diabetic patients: A systematic review and dose‐response meta‐analysis of randomized controlled trials

Water‐soluble dietary fibers have been shown to improve lipid profile and glucose metabolism in diabetes. The aim of this study was to review the effects of psyllium consumption on weight, body mass index, lipid profiles, and glucose metabolism in diabetic patients in randomized controlled trials. A comprehensive systematic search was performed in PubMed/MEDLINE, Web of Sciences, Cochrane, and Scopus by two independent researchers up to August 2019 without any time and language restrictions. The DerSimonian and Laird random‐effects model method performed to calculate the pooled results. Inclusion criteria were randomized controlled trial design, adult subjects, and studies reporting the mean differences with the 95% confidence interval for outcome. Eight studies containing nine arms with 395 participants were identified and included in final analysis. Combined results found a significant reduction in triglycerides, low‐density lipoprotein, fasting blood sugar, and hemoglobin A1c following psyllium consumption (weighted mean differences [WMD]: −19.18 mg/dl, 95% CI [−31.76, −6.60], I2 = 98%), (WMD: −8.96 mg/dl, 95% CI [−13.39, −4.52], I2 = 97%), (WMD: −31.71 ml/dl, 95% CI [−50.04, −13.38], I2 = 97%), and (WMD: −0.91%, 95% CI [−1.31, −0.51], I2 = 99%), respectively. There was no significant change in high‐density lipoprotein, body mass index, cholesterol, and weight. In conclusion, the results demonstrated a significant reduction in triglycerides, low‐density lipoprotein, fasting blood sugar, and hemoglobin A1c by psyllium intervention among diabetic patients.
The use of biophysical approaches to understand ciliary beating

Motile cilia are a striking example of the functional cellular organelle, conserved across all the eukaryotic species. Motile cilia allow the swimming of cells and small organisms and transport of liquids across epithelial tissues. Whilst the molecular structure is now very well understood, the dynamics of cilia is not well established either at the single cilium level nor at the level of collective beating. Indeed, a full understanding of this requires connecting together behaviour across various lengthscales, from the molecular to the organelle, then at the cellular level and up to the tissue scale. Aside from the fundamental interest in this system, understanding beating is important to elucidate aspects of embryonic development and a variety of health conditions from fertility to genetic and infectious diseases of the airways.
Effect of Vitamin D on Blood Pressure and Hypertension in the General Population: An Update Meta-Analysis of Cohort Studies and Randomized Controlled Trials

Background The effect of vitamin D supplementation on blood pressure has been explored in previous meta-analyses, but whether the association is causal in the general population is still unknown. We evaluated the association comprehensively and quantitatively. Methods We searched PubMed and Embase for relevant cohort studies and randomized controlled trials (RCTs). We used a 2-step generalized least-squares method to assess the dose–response association of circulating 25-hydroxyvitamin D (25[OH]D) and hypertension and a fixed-effects model to pool the weighted mean differences (WMDs) and corresponding 95% confidence intervals (95% CIs) of blood pressure across RCTs. Results We identified 11 cohort studies and 27 RCTs, with 43,320 and 3,810 participants, respectively. The dose–response relationship between circulating 25(OH)D levels and hypertension risk was approximately L-shaped (P nonlinearity = .04), suggesting that the risk of hypertension increased substantially below 75 nmol/L as 25(OH)D decreased, but it remained significant over the range of 75–130 nmol/L. However, pooled results of RCTs showed that there was no significant reduction in systolic blood pressure (WMD, −0.00 mm Hg; 95% CI, −0.71 to 0.71) or diastolic blood pressure (WMD, 0.19 mm Hg; 95% CI, −0.29 to 0.67) after vitamin D intervention. Conclusions The results of this meta-analysis indicate that supplementation with vitamin D does not lower blood pressure in the general population. RCTs with long-term interventions and a sufficient number of participants who have low levels of vitamin D are needed to validate these findings.
Rapid hamstrings to quadriceps ratio at long muscle lengths in professional football players with previous hamstring strain injury

Abstract Hamstring strain injuries (HSI) are the most common injury in male professional football and are potentially a primary risk factor to re-injury. Although the isokinetic strength ratios have often been used to identify strength imbalances that can augment the risk of injury in football players, the rate of torque development hamstring to quadriceps ratio (RTD H/Q) has rarely been considered in previous reports. Furthermore, little consideration has been given to selective hamstring lengths (30° of knee flexion) and its influence on torque production. The aim of this study was to investigate the RTD H/Q at long hamstring lengths, conventional (concentric/concentric) and functional (eccentric/concentric) H/Q ratios in football players with and without previous HSI. Twenty-four professional male football players (12 and 12 without previous HSI) performed maximal voluntary isometric contractions at long hamstring lengths (knee and hip flexed at 30° and 85°, respectively) and isokinetic concentric and eccentric contractions at 180°. s−1 and 60°. s−1. Conventional and functional H/Q ratios based on peak torque throughout the entire isokinetic range of motion and at long hamstring lengths were calculated. The RTD H/Q was extracted at long hamstring lengths in incrementing time periods of 50 milliseconds (ms) from the onset of contraction (50–250 ms). No significant differences were found between groups in any H/Q ratios studied. However, small effects (d = 0.4) were found in previously injured hamstrings to lower RTD H/Q at 50 ms and flexor eccentric torque. Previous HSI group showed small to moderate (0.4 > d < 0.6) higher RTD H/Q in late time intervals (>100 ms).