Miracle-dz/UniFAR-FTUs huggingface dataset:
SIRT3 inhibits cardiac hypertrophy by regulating PARP-1 activity

Sirtuin 3 (SIRT3) is a type III histone deacetylase that inhibits cardiac hypertrophy. It is mainly localized in the mitochondria and is thus implicated in mitochondrial metabolism. Recent studies have shown that SIRT3 can also accumulate in the nuclear under stressed conditions, and participated in histone deacetylation of target proteins. Poly [ADP-ribose] polymerase 1 (PARP-1) functions as an important PARP isoform that was involved in cardiac hypertrophy. Our experiments showed that SIRT3 accumulated in the nuclear of cardiomyocytes treated with isoproterenol or SIRT3 overexpression. Moreover, overexpression of SIRT3 by adenovirus inhibited the expression of cardiac hypertrophic genes-ANF and BNP, as well as abrogating PARP-1 activation induced by isoproterenol or phenylephrine. In addition, co-immunoprecipitation experiments revealed that SIRT3 could interact with PARP-1, and overexpression of SIRT3 could decrease the acetylation level of PARP-1. Our results indicate that SIRT3 exerts protective effects against cardiac hypertrophy by reducing the level of acetylation and activity of PARP-1, thus providing novel mechanistic insights into SIRT3-mediated cardiprotective actions.
The seroprevalence of brucellosis and molecular characterization of Brucella species circulating in the beef cattle herds in Albania

Background Brucellosis is a ubiquitous zoonotic disease globally. It is endemic among bovines, sheep, and goats in Albania. The national control and eradication programs for brucellosis has been applied on sheep and goat farms as well as large dairy cattle farms, i.e., those with more than ten milking cows. The current study aims at estimating the herd and average individual animal prevalence of brucellosis in the national beef cattle herds, the missing information that was essential to propose the most appropriate control measures for this sub-population. Rose Bengal Test (RBT), Fluorescence Polarization Assay (FPA), and Enzyme-Linked Immunosorbent Assay (ELISA) were used as serological tests and classical bacteriology for isolation. Results were also used to investigate the difference in sensitivity between the assays used. Methodology In total, 655 animals from 38 beef cattle herds from six southern districts of Albania were sampled. Sera were tested using RBT, FPA, and ELISA. Fifteen positive cows and a bull from eight high-prevalence positive herds were slaughtered, and particular tissue samples were collected for bacteriology. Results The overall herd seroprevalence in the tested beef cattle population was 55%, while the overall average within-herd prevalence (including only positive herds) was 38.3%, 42.7%, and 45.6% determined by the RBT, ELISA, and FPA, respectively. FPA was used for the first time in the diagnosis of bovine brucellosis in Albania, and its sensitivity was higher than RBT and ELISA. Three B. abortus strains were identified, two from the supra-mammary lymph node of two cows and one from the epididymis of a seropositive bull. Conclusion Brucellosis was highly prevalent in beef cattle in the southern part of Albania, and B. abortus was isolated from this subpopulation. To the best of our knowledge, this is the first statistically based survey of bovine brucellosis in beef herds in Albania. Using the FPA in parallel with other serological tests improved overall diagnostic sensitivity. Test and slaughter policy is not a rational approach for the control of brucellosis in beef cattle in Albania, and vaccination is only applicable, including strict control of the movement of animals.
Validity of Algorithms for Identification of Individuals Suffering from Chronic Noncancer Pain in Administrative Databases: A Systematic Review

Abstract Background Secondary analysis of health administrative databases is indispensable to enriching our understanding of health trajectories, health care utilization, and real-world risks and benefits of drugs among large populations. Objectives This systematic review aimed at assessing evidence about the validity of algorithms for the identification of individuals suffering from nonarthritic chronic noncancer pain (CNCP) in administrative databases. Methods Studies reporting measures of diagnostic accuracy of such algorithms and published in English or French were searched in the Medline, Embase, CINAHL, AgeLine, PsycINFO, and Abstracts in Social Gerontology electronic databases without any dates of coverage restrictions up to March 1, 2018. Reference lists of included studies were also screened for additional publications. Results Only six studies focused on commonly studied CNCP conditions and were included in the review. Some algorithms showed a ≥60% combination of sensitivity and specificity values (back pain disorders in general, fibromyalgia, low back pain, migraine, neck/back problems studied together). Only algorithms designed to identify fibromyalgia cases reached a ≥80% combination (without replication of findings in other studies/databases). Conclusions In summary, the present investigation informs us about the limited amount of literature available to guide and support the use of administrative databases as valid sources of data for research on CNCP. Considering the added value of such data sources, the important research gaps identified in this innovative review provide important directions for future research. The review protocol was registered with PROSPERO (CRD42018086402).
MiR-509-3-5p-NONHSAT112228.2 Axis Regulates p21 and Suppresses Proliferation and Migration of Lung Cancer Cells.

BACKGROUND Although the involvement of individual microRNA and lncRNA in the regulation of p21 expression has largely been evidenced, less is known about the roles of functional interactions between miRNAs and lncRNAs in p21 expression. Our previous work demonstrated that miR-509-3-5p could block cancer cell growth. METHOD To gain an insight into the role of miR-509-3-5p in the regulation of p21 expression, we performed in silico prediction and showed that miR-509-3-5p might target the NONHSAT112228.2, a sense-overlapping lncRNA transcribed by a noncode gene overlapping with p21 gene. Mutation and luciferase reporter analysis suggested that miR-509-3-5p could target NONHSAT112228.2, thereby blocked its expression. Consistently, NONHSAT112228.2 expression was inversely correlated with both miR-509-3-5p and p21 expression in cancer cells. Ectopic expression of miR-509-3-5p and knockdown of NONHSAT112228.2 both promoted proliferation and migration of cancer cells. RESULT Interestingly, high-expression of NONHSAT112228.2 accompanied by low-expression of p21 was observed in lung cancer tissues and associated with lower overall survival. CONCLUSION Taken together, our study find a new regulatory pathway of p21, in which miR-509-3-5p functionally interacts with NONHSAT112228.2 to release p21 expression. MiR-509-3-5p-NONHSAT112228.2 regulatory axis can inhibit proliferation and migration of lung cancer cells.
Design and Simulation of Manual Rack and Pinion Steering System

AbstractManual rack and pinion steering systems are commonly used due to their simplicity in construction and compactness. The main purpose of this paper is to design and manufacturemanual rack and pinion steering system according to the requirement of the vehicle for better manoeuvrability. Quantities like turning circle radius, steering ratio, steering effort, etc. are inter-dependent on each other and therefore there are different design consideration according to the type of vehicle. The comparison of resultis shown using tables which will help to design an effective steering for the vehicle. A virtual rack and pinion assembly can be created using software like SOLIDWORKS and ANSYS.
Nature Inspired Meta-heuristic dragonfly Algorithms for Solving Optimal Power Flow Problem

This paper presents a nature inspired metaheuristic based optimization technique, Dragonfly Algorithm (DA) for handling optimal power flow (OPF) problem in electrical power system. In the proposed algorithm (DA), Dragonfly Algorithm has been implemented for finding the optimal setting of the power system control variables for the OPF problem. Minimization of fuel cost, improvement of voltage profile, power loss minimization and emission cost minimization are considered as the objectives for solving the OPF problem. The effectiveness and validity of the proposed Dragonfly Algorithm has been tested by solving OPF problem for above mentioned objectives in the standard IEEE 30-bus test system. The results obtained are compared with the results reported in the literature. The comparison clearly shows the superiority of the proposed Dragonfly Algorithm over the other heuristic techniques applied for solving optimal power flow problem.
Resolving the Spatial Profile of Figure Enhancement in Human V1 through Population Receptive Field Modeling

The detection and segmentation of meaningful figures from their background is one of the primary functions of vision. While work in nonhuman primates has implicated early visual mechanisms in this figure–ground modulation, neuroimaging in humans has instead largely ascribed the processing of figures and objects to higher stages of the visual hierarchy. The detection and segmentation of meaningful figures from their background is one of the primary functions of vision. While work in nonhuman primates has implicated early visual mechanisms in this figure–ground modulation, neuroimaging in humans has instead largely ascribed the processing of figures and objects to higher stages of the visual hierarchy. Here, we used high-field fMRI at 7 Tesla to measure BOLD responses to task-irrelevant orientation-defined figures in human early visual cortex (N = 6, four females). We used a novel population receptive field mapping-based approach to resolve the spatial profiles of two constituent mechanisms of figure–ground modulation: a local boundary response, and a further enhancement spanning the full extent of the figure region that is driven by global differences in features. Reconstructing the distinct spatial profiles of these effects reveals that figure enhancement modulates responses in human early visual cortex in a manner consistent with a mechanism of automatic, contextually driven feedback from higher visual areas. SIGNIFICANCE STATEMENT A core function of the visual system is to parse complex 2D input into meaningful figures. We do so constantly and seamlessly, both by processing information about visible edges and by analyzing large-scale differences between figure and background. While influential neurophysiology work has characterized an intriguing mechanism that enhances V1 responses to perceptual figures, we have a poor understanding of how the early visual system contributes to figure–ground processing in humans. Here, we use advanced computational analysis methods and high-field human fMRI data to resolve the distinct spatial profiles of local edge and global figure enhancement in the early visual system (V1 and LGN); the latter is distinct and consistent with a mechanism of automatic, stimulus-driven feedback from higher-level visual areas.
Galectin-3 as a Next-Generation Biomarker for Detecting Early Stage of Various Diseases

Galectin-3 is a β-galactoside-binding lectin which is important in numerous biological activities in various organs, including cell proliferation, apoptotic regulation, inflammation, fibrosis, and host defense. Galectin-3 is predominantly located in the cytoplasm and expressed on the cell surface, and then often secreted into biological fluids, like serum and urine. It is also released from injured cells and inflammatory cells under various pathological conditions. Many studies have revealed that galectin-3 plays an important role as a diagnostic or prognostic biomarker for certain types of heart disease, kidney disease, viral infection, autoimmune disease, neurodegenerative disorders, and tumor formation. In particular, it has been recognized that galectin-3 is extremely useful for detecting many of these diseases in their early stages. The purpose of this article is to review and summarize the recent literature focusing on the biomarker characteristics and long-term outcome predictions of galectin-3, in not only patients with various types of diseases, but associated animal models.
Examining factors associated with child care utilization patterns in a Canadian urban setting and understanding the association between child care ratios and child outcomes

This dissertation is comprised of three separate manuscripts, each focusing on a unique aspect of child care. Together, they examine both family and child characteristics that are associated with parental decisions regarding child care and provide a better understanding of the impact of child care on children. The first two studies use a longitudinal sibling study consisting of 716 children within 358 families to investigate betweenand within-family variables that are associated with child care use, stability, and type. A specific focus is on ‘going beyond’ typical demographic variables by including two aspects of the psychosocial family environment (i.e., maternal depression and marital conflict). Associations between stability of care and type of setting and children’s language and social-emotional behaviour are also explored. The third paper focuses exclusively on studying the effects of child-staff ratios on child development by conducting a comprehensive systematic review and meta-analysis of published research. This dissertation provides badly needed utilization data for Canada. It also highlights important differences in child care utilization patterns between the current Canadian sample and U.S. data and therefore calls into question the appropriateness of generalizing U.S. findings to the Canadian context. Inferential results indicate that child care use, stability, and type are associated
The Impact of Implant–Abutment Connection on Clinical Outcomes and Microbial Colonization: A Narrative Review

Introduction: Osseointegration are often suffering from oral conditions, especially, the micro gap at the implant–abutment connection represents a site for bacterial plaque aggregation, leading to increased inflammatory cells and causing peri-implantitis. Aim: The aim of this narrative review was to describe the different kinds of implant–abutment connections and their ability to reduce bacterial leakage and thus prevent peri-implantitis. Materials and methods: The following databases were consulted: Pubmed, Scopus, Cochrane Library, and Research gate and a total of 528 articles were found. After reading the abstract and titles, 473 items were excluded. The remaining articles (n = 55) were assessed for full-text eligibility. Thirty-three studies were included in the review. Results and Conclusions: We selected 22 clinical trials and 11 reviews, examining a total sample of 2110 implants. From the review, it was clear that there exists a relationship between the implant–abutment interface and bacterial leakage. All the connections presented an amount of micro-gap and bacterial micro-leakage, though conical and mixed connection systems seemed to behave better. Moreover, both connections seemed to have a better load distribution and the mixed system also had anti-rotational properties which are very useful during the positioning of the prosthesis.
Leakage Power Reduction in Clustered Sleep Transistors

60 Abstract Low-power design has changed the design and verification paradigm forever. The industry is in dire need of a systematic approach to low-power verification. Power consumption of Very Large Scale Integrated (VLSI) circuits has been growing at an alarmingly rapid rate. This increase in power consumption, coupled with the increasing demand for portable/hand-held electronics, has made power consumption a dominant concern in the design of VLSI circuits today. Both leakage and dynamic power are increasing with every switch to smaller process geometries. However, due to process scaling trends, leakage power has become a major component of the total power consumption in VLSI circuits. Here in this paper we aimed to discuss some leakage power reduction techniques. One of the technique to reduce the leakage of a circuit is MTCMOS (Multi-threshold CMOS) approach is discussed.
Are marine n-3 fatty acids protective towards insulin resistance? From cell to human

Marine n-3 fatty acids improve most of the biochemical alterations associated with insulin resistance (IR). Experimental models of dietary-induced IR in rodents have shown their ability (often at a very high dose) to prevent IR, but with sometimes a tissue specific effect. However, in a high sucrose diet-induced IR rat model, they are unable to reverse IR once installed; in other rodent models (dexamethasone, Zucker rats), they are inefficacious perhaps because of the severity of IR. The very low incidence of type-2 diabetes (T2D) in Inuits in the 1960s, which largely increased over the following decades in parallel to the replacement of their traditional marine food for a western diet strongly suggests a protective effect of marine n-3 towards the risk of T2D; this was confirmed by reversal of its incidence in intervention studies reintroducing their traditional food. In healthy subjects and insulin-resistant non-diabetic patients, most trials and meta-analyses conclude to an insulin-sensitising effect and to a very probable preventive or alleviating effect towards IR. Concerning the risk of T2D, concordant data allow us to conclude the protective effect of marine n-3 in Asians while suspicion exists of an aggravation of risk in Westerners, but with the possibility that it could be explained by a high heterogeneity of studies performed in this population. Some longitudinal cohorts in US/European people showed no association or a decreased risk. Further studies using more homogeneous doses, sources of n-3 and assessment of insulin sensitivity methods are required to better delineate their effects in Westerners.
Reduced RANBP9 expression is associated with poor prognosis in colorectal cancer patients

Background Colorectal cancer (CRC) is the third most common malignancy worldwide. RANBP9 is a RAN-binding protein that has been reported to be a reliable predictor for prognosis in some human cancers. The mechanism of RANBP9 involvement in CRC carcinogenesis is unknown. This study measured RANBP9 expression levels in CRC to determine its association with clinicopathological parameters. Methods This study included 228 CRC patients who underwent radical resection. RANBP9 expression was determined using immunohistochemistry. Based on follow-up data, the correlation of RANBP9 expression levels with clinicopathological parameters, including disease free survival (DFS) and overall survival (OS) was evaluated. Results Reduced RANBP9 expression was correlated with tumor location (P=0.014), vascular invasion (P=0.057) and normal serum carcinoembryonic antigen levels (P=0.001). Patients with reduced RANBP9 expression had a 5-year DFS rate of 63.0% compared to 78.9% for patients with high expression levels of RANBP9 (P=0.015). Subgroup analysis demonstrated that reduced RANBP9 expression was significantly correlated with a worse DFS rate (P=0.037) for patients with left-sided colon cancer. RANBP9 was found to be an independent predictive factor for estimating DFS rate (P=0.029, hazard ratio: 0.580, 95% confidence interval: 0.356–0.946) and OS. Conclusions RANBP9 expression levels is a potential prognostic factor for estimating CRC survival rates in patients after surgery.
血友病A患儿凝血因子Ⅷ抑制物相关临床研究

目的 探讨血友病A（HA）患儿凝血因子Ⅷ（FⅧ）抑制物产生的相关因素及抑制物产生前后出血与关节病表现的差异。 方法 对2015年1月至2018年8月河南省血友病管理中心登记收治的381例16岁以下HA患儿进行回顾性分析。 结果 381例HA患儿中，轻型116例（30.4%），中间型196例（51.4%），重型69例（18.1%）。 FⅧ抑制物阳性患者54例（14.2%），高滴度、低滴度分别为22、32例。 血友病家族史与FⅧ抑制物阳性相关[P<0.001，OR=3.299（95%CI 1.743~5.983）]；高强度暴露与FⅧ抑制物的产生相关[P=0.002，OR=2.587（95%CI 1.414~4.731）]。 高强度暴露与高滴度FⅧ抑制物产生相关[P=0.001，OR=8.689（95%CI 2.464~30.638）]。 54例HA患者产生抑制物后，总体关节年出血率、创伤性年出血率增加（z=−3.440，P=0.001；z=−2.232，P=0.026），而非关节年出血率、自发性年出血率与抑制物产生前比较差异无统计学意义（z=−1.342，P=0.180；z=−1.414，P=0.157）。 关节超声评分较产生抑制物前差异无统计学意义（z=−0.632，P=0.527）。 结论 血友病家族史、高强度暴露可增加HA患儿发生FⅧ抑制物的风险，且高强度暴露可增加HA患者出现高滴度抑制物的风险。
AUDIOLOGICAL EVALUATION OF PATIENTS WITH DILATED CARDIOMYOPATHY

A decrease in the blood supply to the cochlea as a result of cardiovascular disease may result in cochlear degeneration and will disrupt the physical and chemical processes in the cochlea. This, in turn, may lead to hearing impairment. So this study was designed to evaluate auditory disorders in patients with dilated cardiomyopathy and to correlate the degree and configuration of hearing loss with the degree and duration of dilated cardiomyopathy. This study was carried out on 20 subjects 10 control subjects and 10 with dilated cardiomyopathy. Full history, clinical examination and audiological evaluation including basic tests and DPOAE and neuro otological ABR was done. Eighty percent of 10 patients with DCM had bilateral SNHL ranged from mild to moderately severe; affecting all frequencies to the greatest degree at high frequencies and 20% (2 patients) had bilateral normal hearing sensitivity. DPOAE were present in all cases of control group and 2 cases with normal hearing from the study group, the remaining cases of study group partially pass indicating impaired cochlear function in study group. Results of (ABR) measurement showed that normal absolute and inter peak latencies in control and study groups. The duration of the disease, have no effect on degree of hearing loss, absolute and inter peak latencies of ABR.
Protective Effect of Curcumin Supplementation and 8 Weeks of Endurance Training on the Antioxidant Index of the Liver of Rats

Background and Objectives : Curcumin as a natural antioxidant is used in some studies. On the other hand, regarding the limited number of studies related to consumption of curcumin in exercise, and according to the role of the liver as the tissue involved in oxidative stress in athletes, in the present study, the effect of 8 weeks of endurance training and curcumin supplementation of antioxidants (superoxide dismutase, glutathione peroxidase and catalase) on the liver of rats was studied. Materials and Methods : Thirteen male Wistar rats were randomly divided into four groups as control (n=6), training (n=9), curcumin (n=9), and curcumin-training (n=9). The training groups underwent an endurance training program for 8 weeks. The exercise program running on a treadmill with no incline for 5 days per week for 30-70 minutes was conducted. The rats in curcumin and curcumin-training groups received 30 mg per kg of body weight of the injected curcumin solution for 3 days a week. 48 hours after the last training session, samples of tissues were collected for the GPX, CAT and SOD measurements. Data were analyzed using Two- way ANOVA test at α≤0.05 . Results : Eight weeks consumption of curcumin caused a significant increase on the level of superoxide dismutase and glutathione peroxidase enzymes in the curcumin group (p ≤0.003). Also there was a significant increase on glutathione peroxidase enzyme in the curcumin-training group (p ≤0.001). However, effect of curcumin supplementation along with endurance training on catalase and superoxide dismutase was not significant. Conclusion : The findings suggest that Curcumin intake along with endurance training can maintain or increase enzymatic antioxidant defense in the liver of rats.