Miracle-dz/UniFAR-FTUs huggingface dataset:
Multi-Modal Nano Particle Labeling of Neurons

The development of imaging methodologies for single cell measurements over extended timescales of up to weeks, in the intact animal, will depend on signal strength, stability, validity and specificity of labeling. Whereas light-microscopy can achieve these with genetically-encoded probes or dyes, this modality does not allow mesoscale imaging of entire intact tissues. Non-invasive imaging techniques, such as magnetic resonance imaging (MRI), outperform light microscopy in field of view and depth of imaging, but do not offer cellular resolution and specificity, suffer from low signal-to-noise ratio and, in some instances, low temporal resolution. In addition, the origins of the signals measured by MRI are either indirect to the process of interest or hard to validate. It is therefore highly warranted to find means to enhance MRI signals to allow increases in resolution and cellular-specificity. To this end, cell-selective bi-functional magneto-fluorescent contrast agents can provide an elegant solution. Fluorescence provides means for identification of labeled cells and particles location after MRI acquisition, and it can be used to facilitate the design of cell-selective labeling of defined targets. Here we briefly review recent available designs of magneto-fluorescent markers and elaborate on key differences between them with respect to durability and relevant cellular highlighting approaches. We further focus on the potential of intracellular labeling and basic functional sensing MRI, with assays that enable imaging cells at microscopic and mesoscopic scales. Finally, we illustrate the qualities and limitations of the available imaging markers and discuss prospects for in vivo neural imaging and large-scale brain mapping.
Circularly-Polarized Beam-Controlling Metalens

In this paper, we present a three-layer flat transmission-type circularly polarized metasurface based on Generalized Snell's Law, for beam controlling at 35GHz. Firstly, metasurface is designed to refract normally incident circularly-polarized plane wave by 27 ° and 42 °, respectively. Secondly, the electromagnetic waves can be focused at a distance of 9$\lambda$ away from the metasurface, and the focal spot radius is about 0.4$\lambda$. It is shown that the metasurface will provide a new beam control method for imaging applications to achieve nearly diffraction-limited imaging performance and miniaturized devices.
Synthèse, optimisation et prototypage d'une main robotique sous-actionnée à cinq doigts

Cette these presente les travaux de recherche doctorale en genie mecanique de Louis- Alexis Allen Demers effectues au Laboratoire de robotique de l’Universite Laval sous la supervision du professeur Clement Gosselin. Cette etude comporte la synthese, l’optimisation et le prototypage d’une main robotique sous-actionnee a cinq doigts. Le principe du sousactionnement mecanique est utilise pour developper une main robotique qui a des capacites de prehension similaires a la main humaine, tout en limitant le nombre d’actionneurs requis pour la commander. Cette main a des dimensions semblables a la main humaine de maniere a ce qu’elle soit fonctionnelle dans un milieu concu pour les humains. De plus, une adaptation de la prise de la main est possible, autant en modifiant la force de prehension que la position des doigts et du pouce. L’analyse statique d’un doigt sous-actionne est d’abord effectuee. Plus particulierement, les conditions permettant d’atteindre une prise stable pour laquelle toutes les forces de contact sont positives sont obtenues. Ainsi, les valeurs optimales pour les dimensions des poulies sont determinees en fonction des longueurs des phalanges et des distributions elementaires de forces etablies. En utilisant ces deux distributions complementaires efficacement, l’ejection de l’objet a saisir par un doigt a trois phalanges et deux reseaux de tendons est evitee. Par la suite, le probleme general de positionnement d’un axe de rotation permettant d’obtenir des poses initiale et finale imposees est resolu. Cette analyse geometrique permet de concevoir un systeme de rotation du pouce afin d’obtenir les differentes configurations typiques de la main humaine. Un systeme d’abduction des doigts soit completement plan, soit un hybride spherique-plan est ensuite couple a ce mecanisme. Ces architectures sont analysees afin de s’assurer d’obtenir les amplitudes d’abduction imposees ainsi que de minimiser l’erreur de coordination entre les metacarpiens. En dernier lieu, les differents mecanismes concus dans cette these sont fabriques, en plus d’un prototype de poignet et de systeme de sous-actionnement entre les doigts. Le prototype de main integrant toutes ces parties est finalement utilise pour prendre differents types d’objets. %%%%This thesis presents the doctoral research in Mechanical Engineering of Louis-Alexis Allen Demers completed at the Laboratoire de robotique of Universite Laval under the supervision of Prof. Clement Gosselin. This project covers the synthesis, optimization and prototyping of a five-finger robotic hand. The principles of mechanical underactuation are used to develop a robotic hand that has grasping capabilities similar to that of the human hand, while limiting the number of actuators required to control it. This hand has dimensions similar to those of the human hand in order to be functional in an environment designed for humans. In addition, an adaptation of the hand grip is possible, either by changing the grasping force or by varying the position of the fingers and thumb. A static…
Management of primary retroperitoneal synovial sarcoma: A case report and review of literature

BACKGROUND Synovial sarcoma (SS) is a rare type of soft tissue sarcoma that is usually developed from areas where synovial tissue exists, especially at the extremities. Nevertheless, several cases of retroperitoneal SS (RSS) have been described. We herein report a case of RSS presented in our institution. CASE SUMMARY A 69-year-old female patient was admitted with a large, palpable, firm mass in the right abdominal space SS. Computerized tomography scan depicted a concentric, sharply marinated retro-peritoneal lesion which was displacing the right kidney and the lower edge of the liver. Subsequently, the patient underwent surgical excision of the mass with additional right nephrectomy and resection of the right adrenal gland and a part of the diaphragm. The final histological diagnosis of the tumour was grade II monophasic RSS. CONCLUSION RSS is encountered in the biphasic type, the monophasic fibrous, and the monophasic epithelial category as well. Relevant clinical manifestations are not always documented at early stages. Therefore, the final diagnosis is posed after complete histological examination taking into consideration the results of immunochemistry and genetic analysis. Therapeutic approach happens often late when metastases at the lungs and the liver are apparent. Thus, 5-year survival rates remain low.
Maternal One-Carbon Metabolism and Infant DNA Methylation between Contrasting Seasonal Environments: A Case Study from The Gambia

Abstract Background The periconceptional period is a time in which environmentally induced changes to the epigenome could have significant consequences for offspring health. Metastable epialleles (MEs) are genomic loci demonstrating interindividual variation in DNA methylation with intraindividual crosstissue correlation, suggesting that methylation states are established in the very early embryo before gastrulation. In our previous Gambian studies, we have shown that ME methylation states in the offspring are predicted by maternal concentrations of certain nutritional biomarkers around the time of conception. Objective We aimed to assess whether the profile of maternal biomarker predictors of offspring methylation differs between rainy and dry seasons in a population of rural Gambians, using a larger set of 50 recently identified MEs. Methods We measured 1-carbon biomarkers in maternal plasma back-extrapolated to conception, and cytosine-phosphate-guanine (CpG) methylation at 50 ME loci in their infants’ blood at a mean age of 3.3 mo (n = 120 mother-child pairs). We tested for interactions between seasonality and effects of biomarker concentrations on mean ME methylation z score. We used backward stepwise linear regression to select the profile of nutritional predictors of methylation in each season and repeated this analysis with biomarker principal components (PCs) to capture biomarker covariation. Results We found preliminary evidence of seasonal differences in biomarker-methylation associations for folate, choline, and homocysteine (interaction P values ≤0.03). Furthermore, in stratified analyses, biomarker predictors of methylation changed between seasons. In the dry season, vitamin B-2 and methionine were positive predictors. In the rainy season, however, choline and vitamin B-6 were positive predictors, and folate and vitamin B-12 were negative predictors. PC1 captured covariation in the folate metabolism cycle and predicted methylation in dry season conceptions. PC2 represented the betaine remethylation pathway and predicted rainy season methylation. Conclusions Underlying nutritional status may modify the association between nutritional biomarkers and methylation, and should be considered in future studies.
Automated Classification of Pulmonary Nodules through a Retrospective Analysis of Conventional CT and Two-phase PET Images in Patients Undergoing Biopsy

Objective(s): Positron emission tomography/computed tomography (PET/CT) examination is commonly used for the evaluation of pulmonary nodules since it provides both anatomical and functional information. However, given the dependence of this evaluation on physician’s subjective judgment, the results could be variable. The purpose of this study was to develop an automated scheme for the classification of pulmonary nodules using early and delayed phase PET/CT and conventional CT images. Methods: We analysed 36 early and delayed phase PET/CT images in patients who underwent both PET/CT scan and lung biopsy, following bronchoscopy. In addition, conventional CT images at maximal inspiration were analysed. The images consisted of 18 malignant and 18 benign nodules. For the classification scheme, 25 types of shape and functional features were first calculated from the images. The random forest algorithm, which is a machine learning technique, was used for classification. Results: The evaluation of the characteristic features and classification accuracy was accomplished using collected images. There was a significant difference between the characteristic features of benign and malignant nodules with regard to standardised uptake value and texture. In terms of classification performance, 94.4% of the malignant nodules were identified correctly assuming that 72.2% of the benign nodules were diagnosed accurately. The accuracy rate of benign nodule detection by means of CT plus two-phase PET images was 44.4% and 11.1% higher than those obtained by CT images alone and CT plus early phase PET images, respectively. Conclusion: Based on the findings, the proposed method may be useful to improve the accuracy of malignancy analysis.
Evaluation of the electromagnetic field intensity in operating rooms and estimation of occupational exposures of personnel

Introduction Operating rooms in hospitals are facilitated with different types of electronic systems, which produce electromagnetic waves. High intensities of magnetic waves may have harmful effects on biological environments. This study aims to evaluate the electromagnetic field intensity at different parts of operating rooms at the first stage and estimate the occupational exposure to operating room personnel at the next phase. Materials and methods At this cross-sectional study, the magnetic field intensity was evaluated using teslameter at several parts of operating rooms, during operating procedures, while electrical instruments were working. Background electromagnetic field intensity was measured when all the electrical systems were idle. Statistical analysis was performed using SPSS software. The results were compared with ICNIRP standards. Results The maximum intensity of magnetic field was measured around high-voltage systems at the distance of 50 cm in the personnel’s standing area at DCR and PCNL operating procedures were 5.9 and 5.6, respectively. The number of on-mode electrical systems was inconsistent with the intensity of electromagnetic fields at the standing area of operating room personnel’s. The intensity of magnetic fields around high-voltage systems, which was about 46.75 mG at the distance of 10 cm, was the highest among measured electromagnetic fields. Conclusions The highest magnetic field intensity measured in this study was related to high-voltage systems and is lower than advised intensity by ICNIRP for occupational exposure. Based on this study, it can be concluded that there are no considerable risks of electromagnetic exposure for operating room personnels.
Complete Genome Sequence of Lactobacillus crispatus CO3MRSI1

Lactobacillus crispatus is a commonly found bacterium in vertebrate microbiota, particularly the human vagina. We report the first complete genome of a strain isolated from a human vagina, L. crispatus CO3MRSI1. ABSTRACT Lactobacillus crispatus is a commonly found bacterium in vertebrate microbiota, particularly the human vagina. We report the first complete genome of a strain isolated from a human vagina, L. crispatus CO3MRSI1.
EGFR deficiency leads to impaired self-renewal and pluripotency of mouse embryonic stem cells

Background Self-renewal and pluripotency are considered as unwavering features of embryonic stem cells (ESCs). How ESCs regulate the self-renewal and differentiation is a central question in development and regenerative medicine research. Epidermal growth factor receptor (EGFR) was identified as a critical regulator in embryonic development, but its role in the maintenance of ESCs is poorly understood. Methods Here, EGFR was disrupted by its specific inhibitor AG1478 in mouse ESCs (mESCs), and its self-renewal and pluripotency were characterized according to their proliferation, expression of pluripotency markers, embryoid body (EB) formation, and mRNA expression patterns. We also used another EGFR inhibitor (gefitinib) and RNA interference assay to rule out the possibility of non-specific effects of AG1478. Results EGFR inhibition by AG1478 treatment in mESCs markedly reduced cell proliferation, caused cell cycle arrest at G0/G1 phase, and altered protein expressions of the cell cycle regulatory genes (CDK2 (decreased 11.3%) and proliferating cell nuclear antigen (decreased 25.2%)). The immunoreactivities and protein expression of pluripotency factors (OCT4 (decreased 26.9%)) also dramatically decreased, while the differentiation related genes (GATA4 (increased 1.6-fold)) were up-regulated in mESCs after EGFR inhibition. Meanwhile, EGFR inhibition in mESCs disrupted EB formation, indicating its impaired pluripotency. Additionally, the effects observed by EGFR inhibition with another inhibitor gefitinib and siRNA were consistent with those observed by AG1478 treatment in mESCs. These effects were manifested in the decreased expression of proliferative and pluripotency-related genes and the increased expression of genes involved in differentiation. Moreover, RNA-seq analysis displayed that transcript profiling was changed significantly after EGFR inhibition by AG1478. A large number of differentially expressed genes were involved in cell cycle, apoptotic process, epigenetic modification, and metabolic process, which were related to self-renewal and pluripotency, confirming that EGFR deficiency impaired self-renewal and pluripotency in mESCs. Conclusions Taken together, our results demonstrated the importance of EGFR in guarding the stemness of mESCs.
Les supraconducteurs : des propriétés aux applications électrotechniques

Les supraconducteurs constituent des materiaux d'avenir pour l'electrotechnique. Les benefices apportes dans les applications, ajoutes au gain de volume et de poids sont des atouts suffisants pour que l'on ne puisse pas faire d'impasse sur les etudes qui les concernent. L'article, loin de donner un panorama complet des applications actuelles et encore moins de celles de demain, souhaite simplement attirer l'attention sur un champ d'etude important pour les laboratoires et avec des developpements industriels tout a fait acceptables. des materiaux nouveaux et une cryogenie de plus en plus simple rendent de nombreuses applications possibles.
Multifaced Roles of the Urokinase System in the Regulation of Stem Cell Niches

Proliferation, subsequent migration to the damaged area, differentiation into appropriate cell types, and/or secretion of biologically active molecules and extracellular vesicles are important processes that underlie the involvement of stem/progenitor cells in the repair and regeneration of tissues and organs. All these functions are regulated through the interaction between stem cells and the microenvironment in the tissue cell niches that control these processes through direct cell-cell interactions, production of the extracellular matrix, release of extracellular vesicles, and secretion of growth factors, cytokines, chemokines, and proteases. One of the most important proteolytic systems involved in the regulation of cell migration and proliferation is the urokinase system represented by the urokinase plasminogen activator (uPA, urokinase), its receptor (uPAR), and inhibitors. This review addresses the issues of urokinase system involvement in the regulation of stem cell niches in various tissues and analyzes the possible effects of this system on the signaling pathways responsible for the proliferation, programmed cell death, phenotype modulation, and migration properties of stem cells.
Design, optimization and validation of genes commonly used in expression studies on DMH/AOM rat colon carcinogenesis model

Colorectal cancer (CRC), also known as colon cancer, is the third most common form of cancer worldwide in men and the second in women and is characterized by several genetic alterations, among them the expression of several genes. 1,2-dimethylhydrazine (DMH) and its metabolite azoxymethane (AOM) are procarcinogens commonly used to induce colon cancer in rats (DMH/AOM rat model). This rat model has been used to study changes in mRNA expression in genes involved in this pathological condition. However, a lack of proper detailed PCR primer design in the literature limits the reproducibility of the published data. The present study aims to design, optimize and validate the qPCR, in accordance with the MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines, for seventeen genes commonly used in the DMH/AOM rat model of CRC (Apc, Aurka, Bax, Bcl2, β-catenin, Ccnd1, Cdnk-1A, Cox2, Gsk3beta, IL-33, iNOs, Nrf2, p53, RelA, Smad4, Tnfa and Vegfa) and two reference genes (Actb or β-actin and B2m). The specificity of all primer pairs was empirically validated on agarose gel, and furthermore, the melting curve inspection was checked as was their efficiency (%) ranging from 90 to 110 with a correlation coefficient of r2 > 0.980. Finally, a pilot study was performed to compare the robustness of two candidate reference genes.
Gamma-Carbolines Derivatives As Promising Agents for the Development of Pathogenic Therapy for Proteinopathy

Uncontrolled protein aggregation, accompanied by the formation of specific inclusions, is a major component of the pathogenesis of many common neurodegenerative diseases known as proteinopathies. The intermediate products of this aggregation are toxic to neurons and may be lethal. The development strategy of pathogenic therapy for proteinopathy is based on the design of drugs capable of both inhibiting proteinopathy progression and increasing the survival of affected neurons. The results of a decade-long research effort at leading Russian and international laboratories have demonstrated that Dimebon (Latrepirdine), as well as a number of its derivatives from a gamma-carboline group, show a strong neuroprotective effect and can modulate the course of a neurodegenerative process in both in vitro and in vivo model systems. The accumulated data indicate that gamma-carbolines are promising compounds for the development of pathogenic therapy for proteinopathies.
Treatment of de novo femoro-popliteal lesions with a new Drug Coated Balloon: early experience of a single Center in the first 50 patients

Angioplasty with drug-coated balloon (DCB) is an emerging and reliable method for the treatment of femoro-popliteal lesions. We report our experience with the Stellarex™ DCB in the first 50 patients. Methods From July 2015 to November 2017, 50 patients (41 M, 9F), medium age (64 ± 7.4 year) were subject to 33 angioplasties (PTAs) for femoro-popliteal lesions with a paclitaxel-coated balloon (Stellarex™). Based upon clinical data sixteen patients had severe claudication (56% - Rutherford class 3); ten patients suffered from ischemic rest pain (34% - Rutherford class 4); and five presented minor tissue loss (10% - Rutherford class 5). 42% of patients showed femoro-popliteal lesion TASC-II B, and 58% presented lesions pertaining to TASC-II C. Results Immediate technical success was 100% without perioperative complications. Primary patency rate was 94% at twelve months. In three cases restenosis (6%) was detected within a year from procedure, and a further PTA DCB was performed with primary assisted patency rates of 100% at twelve months. Two patients underwent major lower limb amputation. Three patients died during follow-up and one patient was lost at follow-up. Conclusion DCB angioplasty with Stellarex™ is a viable alternative to traditional endovascular procedures proving satisfactory primary patency rates at twelve months. Based on our experience, treatment with DCB is a first choice technique for non-complex de novo lesions of the femoro – popliteal tract.
A Rare Case of Liver Metastasis from Prostate Cancer Mimicking Hepatocellular Carcinoma on Immunohistochemistry: Role of F-18 Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography in Diagnosis

We present a case with space-occupying lesion in cirrhotic liver, diagnosed as hepatocellular carcinoma on immunohistochemistry, who underwent F-18 fluorodeoxyglucose positron emission tomography/computed tomography (FDG PET/CT) and showed FDG-avid lesions in liver as well as in the prostate. These findings guided in establishing the diagnosis of prostate cancer, metastasizing to liver by performing additional immunohistochemical markers. PET/CT was also useful in identifying coexisting non-Hodgkin's lymphoma.