Miracle-dz/UniFAR-FTUs huggingface dataset:
Integrated approach to produce a recombinant, his‐tagged human α‐galactosidase a in mammalian cells

Successful production of recombinant proteins (r‐proteins) by transient gene expression (TGE) depends on several parameters (including producer cells, culture conditions, transfection procedure, or expression vector) that should be optimized when producing any recombinant product. In this work, TGE‐based production of human α‐galactosidase A (GLA) is described. Producer cells, expression vectors, and parameters influencing cell metabolism after transfection have been tested. The enzyme is secreted, has the right molecular weight, and is enzymatically active. Productivities of up to 30–40 mg/L have been achieved, with a simple, fast procedure. A 6 × His tag allows enzyme purification in a single step, rendering a highly pure product. We propose a TGE‐based protocol able to produce up to several milligrams per liter of highly pure, active GLA in a time as short as a few days. By this, enough amounts of engineered versions of the enzyme can be easily produced to be tested in vitro or in preclinical trials. © 2011 American Institute of Chemical Engineers Biotechnol. Prog. , 2011
PERFORMANCE EVALUATION OF WET SCRUBBER SYSTEM FOR INDUSTRIAL AIR POLLUTION CONTROL

The concentration of pollutants emitted from industrial production are generally toxic and hazardous, which can be a serious health risk to humans not limited to respiratory ailments (asthma, bronchitis, tuberculosis, etc) but also to the photosynthesis in plants. In this study, a pilot scrubber system for PM10 control has been designed using data obtained from cement industry. A model for the overall collection efficiency of counter current scrubber system and Langmuir’s approximations were used to predict the performance of the system by considering droplet sizes of 500µm, 1000µm, 1500µm and 2000µm. The range of liquid to gas ratio recommended by the US Environmental Protection Agency (EPA) has been used to investigate the appropriate ratio for optimum performance of the system. Due to reversed flow in the Langmuir’s approximation, negative collection efficiencies for the 1µm dust particle were obtained. For 5µm and 10µm dust particles, the maximum collection efficiencies were determined to be 99.988% and 100.000% at 500µm droplet size and 2.7 l/m 3 while the minimum was obtained to be 43.808% and 58.728% at 2000µm droplet size and 0.7 l/m 3 . The predicted performance of the scrubber system was then validated using the World Health Organization (WHO) air quality standard for PM10.
The use of mitoxantrone (Novantrone) for the treatment of multiple sclerosis

Mitoxantrone is the first drug approved for the treatment of secondary progressive multiple sclerosis (SPMS) in the United States. This assessment considers use of mitoxantrone in the treatment of MS. Mitoxantrone probably reduces the clinical attack rate and reduces attack-related MRI outcomes in patients with relapsing MS (Type B recommendation). Also, mitoxantrone may have a beneficial effect on disease progression in patients with MS whose clinical condition is worsening (Type B recommendation). The potential for serious toxicity of mitoxantrone, however, must be taken into account when considering this therapy in individual patients. Moreover, because the potential clinical benefits on disease progression appear to be only modest, the results of the single phase III trial should be replicated in another (and hopefully much larger) clinical study before this agent is widely recommended for the treatment of patients with MS.
An Empirical Study on the Interaction between Organizational Innovation and Technological Innovation in Manufacturing Companies

Innovation is necessary for manufacturing enterprises that compete in environments where change is unpredictable, pervasive, and continuous. This paper presents a framework for researching on the relationship between the organizational innovation (OI) and the technological innovation (TI). The study designs a framework that can reflect the harmonious interactive relationship between the two essential factors of the enterprise innovation system. Analyze the harmonious interaction of organizational structure innovation (S-OI) and technological innovation, organizational culture innovation(C-OI) and technological innovation, organizational process innovation (PC-OI) and technological innovation, so as organizational personnel innovation (PN-OI) and technological innovation concretely. And then analyze the relation in the empirical research. Data were obtained from the questionnaire and interviews with managers of the manufacturing enterprises and analysis of statistics and a detailed nominal list of innovation by statistics methods
Toward an Integrative Concept of Control of Total Body Sodium.

Total body sodium (TBSodium) is a major determinant of body water and arterial pressure. Several observations, in particular that of a "sodium memory," indicate that TBSodium is a controlled variable. Various regulatory elements are involved, e.g., the renin-angiotensin-aldosterone system, atrial receptors, and renal arterial pressure. Balance studies in dogs provide new insights into their contributions to TBSodium control.
Comprehensive paternity assignment: genotype, spatial location and social status in song sparrows, Melospiza Melodia

Comprehensive, accurate paternity assignment is critical to answering numerous questions in evolutionary ecology. Yet, most studies of species with extra‐pair paternity (EPP) fail to assign sires to all offspring. Common limitations include incomplete and biased sampling of offspring and males, particularly with respect to male location and social status, potentially biasing estimated patterns of paternity. Studies that achieve comprehensive sampling and paternity assignment are therefore required. Accordingly, we genotyped virtually all males and >99% of 6‐day‐old offspring over 16 years in a song sparrow (Melospiza melodia) population and used three complementary statistical methodologies to attempt complete paternity assignment for all 2207 offspring. Assignments were highly consistent across maximum likelihood methods that used solely genotype data, and heuristic and integrated Bayesian analyses that included data describing individual locations. Sires were assigned to >99% of all genotyped offspring with ≥95% confidence, revealing an EPP rate of c. 28%. Extra‐pair sires primarily occupied territories neighbouring their extra‐pair offspring; spatial location was therefore highly informative for paternity assignment. EPP was biased towards paired territorial males, although unpaired territorial and floater males sired c. 13% of extra‐pair offspring. Failing to sample and include unpaired males as candidate sires would therefore substantially reduce assignment rates. These analyses demonstrate the integration of genetic and ecological information to achieve comprehensive paternity assignment and direct biological insight, illustrate the potential biases that common forms of incomplete sampling could have on estimated patterns of EPP, and provide an essential basis for understanding the evolutionary causes and consequences of EPP.
Time-dependent alterations in ethanol intake in male wistar rats exposed to short and prolonged daily maternal separation in a 4-bottle free-choice paradigm.

BACKGROUND Previous studies have shown that maternal separation can be used in animal studies of early environmental influence on adult ethanol intake. These studies have shown that short daily separations result in low ethanol intake, whereas prolonged separations relate to an enhanced risk for a high ethanol intake. The aim of the present study was to further examine the long-term effects of early-life events on ethanol intake. METHODS Rat pups were exposed to 15 minutes (MS15) or 360 minutes (MS360) of daily maternal separation during postnatal days 1 to 21 or kept under normal animal facility rearing (AFR) conditions. In adulthood, male rats were given free access to 5, 10, and 20% ethanol, in addition to water, in a 4-bottle-choice paradigm. RESULTS No differences in total ethanol intake or preference between the 3 experimental groups were found. The 54-day drinking period was divided into acquisition, stabilization, and maintenance phases for analysis of time and group differences. The MS15 rats increased ethanol intake over time; they mostly consumed 5% ethanol and had a low intake of 20% ethanol throughout the experiment. MS360 rats increased ethanol intake, changed preference from 5% to 20% ethanol, and had a higher increase in intake of 20% ethanol over time. The ethanol intake and preference in the AFR rats were more similar to that of the MS360 rats. CONCLUSIONS Time-dependent changes were observed in the preferred choice of low versus high ethanol concentrations in MS15 and MS360 rats. The results support previous findings suggesting that MS15 can be used as a model for environmental protective factors and that MS360 represents a risk environment for acquisition of a high adult ethanol intake.
A tractable approximation of expectation-based stochastic posynomial programs

In this paper, we consider a specific type of stochastic posynomial programming, which minimizes the expectation of a stochastically perturbed posynomial subject to inequalities of expectations of stochastically perturbed posynomials. Our goal is to build a computationally tractable approximation of this (typically intractable) optimization problem. We show that, under the assumption that the random perturbations are normal and narrow, these expectation-based stochastic posynomial programs can be approximated as conventional (deterministic) posynomial programs, that interior-point methods can solve efficiently
Evidence for an increase in the ozone photochemical lifetime in the eastern United States using a regional air quality model

Measures to control surface ozone rely on quantifying production attributable to local versus regional (upwind) emissions. Here we simulate the relative contribution of local (i.e., within a particular state) and regional sources of surface ozone in the eastern United States (66–94°W longitude) for July 2002, 2011, and 2018 using the Comprehensive Air‐quality Model with Extensions (CAMx). To determine how emissions and chemistry within the domain affect the production, loss, lifetime, and transport of trace gases, we initialize our model with identical boundary conditions in each simulation. We find that the photochemical lifetime of ozone has increased as emissions have decreased. The contribution of ozone from outside the domain (boundary condition ozone, BCO3) to local surface mixing ratios increases in an absolute sense by 1–2 ppbv between 2002 and 2018 due to the longer lifetime of ozone. The photochemical lifetime of ozone lengthens because the two primary gas phase sinks for odd oxygen (Ox ≈ NO2 + O3)—attack by hydroperoxyl radicals (HO2) on ozone and formation of nitrate—weaken with decreasing pollutant emissions. The relative role of BCO3 will also increase. For example, BCO3 represents 34.5%, 38.8%, and 43.6% of surface ozone in the Baltimore, MD, region during July 2002, 2011, and 2018 means, respectively. This unintended consequence of air quality regulation impacts attainment of the National Ambient Air Quality Standard for surface ozone because the spatial and temporal scales of photochemical smog increase; the influence of pollutants transported between states and into the eastern U.S. will likely play a greater role in the future.
Responding maintained under intermittent schedules of electric-shock presentation: "Safety" or schedule effects?

Four experiments were conducted in which lever pressing by squirrel monkeys was maintained under multiple, mixed, or chained schedules of electric-shock presentation. In the first two experiments, a multiple schedule was employed in which a fixed-interval schedule of shock presentation alternated with a signaled two-minute component. Initially, no events were scheduled during the two-minute component (a safety period). In the first experiment, the safety period was "degraded" by introducing and systematically increasing the frequency of periodic shocks presented during that component. In the second experiment, the proportion of overall safe time to unsafe time was decreased by decreasing the value of the fixed-interval schedule while holding constant shock frequency during the two-minute component. In the third experiment, the overall arrangement was changed from a multiple to a mixed schedule in an attempt to determine whether fixed-interval responding would be maintained when a single exteroceptive stimulus was associated with both components. In the fourth experiment, the overall arrangement was changed from a multiple to a chained schedule in an effort to determine whether fixed-interval responding would be maintained when its consequence was presentation of a signaled "unsafe" period. Fixed-interval responding was well maintained under all experimental conditions; the varied relationships obtained lend more support to conceptualizations of shock-maintained behavior as exemplifying schedule-controlled behavior than to suggestions that such behavior may be readily accounted for by "safety theory. "
Platelet response in practice: Applying new insights and tools for testing and treatment

Dr. Deepak Bhatt: We have taken in a wealth of terrifi c information from the three preceding talks in this symposium. Let’s now share some questions from the audience and explore some of the points raised in the preceding talks in a bit more practical detail for clinicians. Our three prior speakers are joined in this panel discussion by Cleveland Clinic’s Dr. Frank Peacock, who brings an emergency medicine perspective. Let’s begin with a case-based question supplied from the audience. The patient is a 42-year-old morbidly obese man without diabetes who had a non-ST-elevation myocardial infarction (MI) less than 1 year ago. A drug-eluting stent was placed at the time of his MI, and now restenosis has occurred. He is on aspirin and clopidogrel 75 mg/day. Do you recommend running a vasodilator-stimulated phosphoprotein (VASP) test and possibly increasing the clopidogrel dose to 150 mg/ day, or should the patient just be switched to prasugrel (assuming it is commercially available) without running the VASP test? I’ll take a quick initial stab at this question. Studies of antiplatelet therapies to prevent in-stent restenosis have been a mixed bag. Some of the trials with glycoprotein IIb/IIIa inhibitors have shown an effect on restenosis, but most have not. Similarly, some of the analyses of the thienopyridines ticlopodine and clopidogrel have shown an effect on restenosis, but most have not. For the most part, restenosis does not appear to be heavily mediated by platelets, at least not in a way that we can infl uence by therapy. On the other hand, stent thrombosis is highly platelet mediated, so I would alter the case to one in which stent thrombosis is the clinical problem. Assuming that the patient has been adherent to his antiplatelet regimen, which tests would you perform, and how would you act on the information from those tests? Dr. Kandice Kottke-Marchant: The 2007 guidelines on acute coronary syndrome (ACS) management from the American College of Cardiology and American Heart Association (ACC/AHA)1 do not address platelet function testing, and almost none of the clinical trials of antiplatelet agents had an arm that included testing and dose adjustment based on platelet function studies. Platelet testing is available at some centers; at Cleveland Clinic, we use platelet aggregation testing. One can do platelet aggregation testing on a patient-by-patient basis; if inhibition appears to be suboptimal, a treatment decision should be made, but there is little guidance from the literature to steer that decision. I have seen clinicians increase the dose of clopidogrel or aspirin in response to platelet function tests, which occasionally triggers a confi rmatory call from the pharmacy department.
Correlations between clinical illness, respiratory virus infections and climate factors in a tropical paediatric population

SUMMARY Weekly (August 2003–December 2008) numbers of five common paediatric diseases and the incidence of respiratory viruses were obtained from a children's hospital in Singapore and correlated with climate data using multivariate time-series techniques. Upper respiratory tract infections were positively correlated with the incidences of influenza A, B, respiratory syncytial virus (RSV) and parainfluenza viruses (types 1–3 combined). Lower respiratory tract infections were positively correlated with only the incidence of RSV. Both upper and lower respiratory tract infections were negatively correlated with relative humidity. Asthma admissions were negatively correlated with maximum temperature and positively correlated with the incidence of influenza B and increasing hours of sunshine. Although sporadic cases of adenovirus infection were identified, not enough cases were available for a more detailed analysis. Gastroenteritis and urinary tract infections, included as control diseases, were not correlated significantly with any climate parameters. These correlations are compatible with current understanding of respiratory virus survival under certain climate conditions and may assist the prediction of disease burdens and hospital resource planning in such tropical environments.
ERECTA controls low light intensity-induced differential petiole growth independent of Phytochrome B and Cryptochrome 2 action in Arabidopsis thaliana

Plants can respond quickly and profoundly to changes in their environment. Several species, including Arabidopsis thaliana, are capable of differential petiole growth driven upward leaf movement (hyponastic growth) to escape from detrimental environmental conditions. Recently, we demonstrated that the leucine-rich repeat receptor-like Ser/Thr kinase gene ERECTA, explains a major effect Quantitative Trait Locus (QTL) for ethylene-induced hyponastic growth in Arabidopsis. Here, we demonstrate that ERECTA controls the hyponastic growth response to low light intensity treatment in a genetic background dependent manner. Moreover, we show that ERECTA affects low light-induced hyponastic growth independent of Phytochrome B and Cryptochrome 2 signaling, despite that these photoreceptors are positive regulators of low light-induced hyponastic growth.
The novel method for Adaptive Image Enhancement Using Histogram Equalization

The article adaptively improves the fingerprint image quality, reduces noise and enhances it. The term adaptive means that the measuring factors of the method are automatically adjusted based on the input fingerprint image. It is a faster means to enhance the fingerprint image for many purposes like matching, orientation estimation etc. Mainly four steps comprise this method, in which there are two forms based on histogram equalization and other based on order of anisotropic filters are applied and a nonlinear dynamic range adjustment method is also used. Keywords— Look Up Table (LUT); anti-symmetric product coding (APC); odd-multiple-storage (OMS; distributed arithmetic (DA);
Approche numérique par éléments discrets 3D de la sollicitation d'un écoulement granulaire sur un obstacle

L'analyse de la pression exercee par un ecoulement granulaire sur un obstacle necessite la connaissance du coefficient de trainee. Neanmoins, les etudes bibliographiques experimentales ne fournissent pas les donnees internes de l'ecoulement intervenant dans son calcul. C'est pourquoi, nous proposons la determination du coefficient de trainee par l'application d'outils numeriques tridimensionnels, bases sur la methode des elements discrets (MED), et valides par des etudes experimentales. L'experience est un canal d'ecoulement de laboratoire, integralement modelise par la MED. Les lois locales de contact integrent un comportement normal elastique, hysteretique associe a un critere de glissement tangentiel. La validation du modele integral repose sur la similarite entre les resultats experimentaux et numeriques des parametres physiques de l'ecoulement et de l'effort d'impact sur l'obstacle. Cette validation est obtenue sans calibration importante des parametres numeriques. Le modele integral est ensuite optimise, en termes de temps de calcul et de possibilites d'etudes, sous la forme d'un canon granulaire, dans lequel n'est simulee que la zone d'interaction entre l'ecoulement et l'obstacle. Les proprietes de l'ecoulement : vitesse, epaisseur, densite, sont controlees car integrees en tant que parametres initiaux numeriques. L'influence de caracteristiques associees a l'ecoulement, comme le nombre de Froude, a la taille des grains, ou a la forme et la taille de l'obstacle, sur le coefficient de trainee, est alors analysee. Enfin, une extension micromecanique est proposee dans l'analyse de l'influence d'une adhesion et d'une cohesion integrees aux lois locales de contact.
Comparative analysis and evaluation of high voltage power generation architectures

This paper introduces the high voltage generation architectures derivation methodology and comparative evaluation of high voltage power generation architectures based on the key performance items such as efficiency, power density, high voltage pulse speed, high voltage pulse ripple, HV insulation and scalability for different output voltage and output power ratings. Based on comparative evaluation of high voltage generation architectures with single inverter configuration, high voltage generation architecture with single inverter, multiple high voltage transformers and multiple stage voltage multiplication circuits overall outperforms other high voltage generation architectures based on architecture performance comparative analysis and evaluation at 100kV/10kW output rating as case study.
Dialogue Summary: Improving Pain and Symptom Management in Cancer Care in Ontario

Participants generally agreed with the challenges presented in the pre-circulated evidence brief, but suggested that there were two overarching challenges that were more important to acknowledge: 1) the current ‘paradigm’ underpinning cancer care in the province is not conducive to comprehensive, patient-centred pain and symptom management across the entire continuum of care; and 2) a persuasive business case for focusing on improving pain and symptom management across the entire continuum of care has not been developed. Four additional, more specific challenges were also discussed: 1) existing pain and symptom management guidelines have not been tailored to meet the diverse needs of patients and their informal/family caregivers, healthcare providers and teams, and the full scope of system settings in which pain and symptom management could be provided; 2) limited attention has been paid to ensuring culturally sensitive engagement of patients and their families; 3) nonphysician healthcare providers have been underutilized, and physicians have not been trained to understand how their role can be complemented by other providers; and 4) funding arrangements are not optimally designed to support the changes needed to underpin improvements. Topic Overview