Miracle-dz/UniFAR-FTUs huggingface dataset:
Body Composition Changes During a 24-h Winter Mountain Running Race Under Extremely Cold Conditions

Background: To date, no study has focused on body composition characteristics and on parameters associated with skeletal muscle damage and renal function in runners participating in a 24-h winter race held under extremely cold environmental conditions (average temperature of -14.3°C). Methods: Anthropometric characteristics, plasma urea (PU), plasma creatinine (Pcr), creatine kinase (CK), plasma volume (PV) and total body water (TBW) were assessed pre- and post-race in 20 finishers (14 men and 6 women). Results: In male runners, body mass (BM) (p = 0.003) and body fat (BF) (p = 0.001) decreased [-1.1 kg (-1.4%) and -1.1 kg (-13.4%), respectively]; skeletal muscle mass (SM) and TBW remained stable (p > 0.05). In female runners, BF decreased (p = 0.036) [-1.3 kg (-7.8%)] while BM, SM and TBW remained stable (p > 0.05). The change (Δ) in BM was not related to Δ BF; however, Δ BM was related to Δ SM [r = 0.58, p = 0.007] and Δ TBW (r = 0.59, p = 0.007). Δ SM correlated with Δ TBW (r = 0.51, p = 0.021). Moreover, Δ BF was negatively associated with Δ SM (r = -0.65, p = 0.002). PV (p < 0.001), CK (p < 0.001), Pcr (p = 0.004) and PU (p < 0.001) increased and creatinine clearance (CrCl) decreased (p = 0.002). The decrease in BM was negatively related to the increase in CK (r = -0.71, p < 0.001). Δ Pcr was positively related to Δ PU (r = 0.64, p = 0.002). The decrease in CrCl was negatively associated with the increase in both PU (r = -0.72, p < 0.001) and CK (r = -0.48, p = 0.032). Conclusion: The 24-h running race under extremely cold conditions led to a significant BF decrease, whereas SM and TBW remained stable in both males and females. Nevertheless, the increase in CK, Pcr and PU was related to the damage of SM with transient impaired renal function.
Brief hypoxia in late gestation sheep causes prolonged disruption of fetal electrographic, breathing behaviours and can result in early labour

Brief episodes of severe fetal hypoxia can arise in late gestation as a result of interruption of normal umbilical blood flow Systemic parameters and blood chemistry indicate complete recovery within 1–2 hours, although the long‐term effects on fetal brain functions are unknown Fetal sheep were subjected to umbilical cord occlusion (UCO) for 10 min at 131 days of gestation, and then monitored intensively until onset of labour or delivery (<145 days of gestation) Normal patterns of fetal behaviour, including breathing movements, episodes of high and low voltage electorcortical activity, eye movements and postural (neck) muscle activity, were disrupted for 3–10 days after the UCO Preterm labour and delivery occurred in a significant number of the pregnancies after UCO compared to the control (sham‐UCO) cohort.
The Association between Substance Use Disorders and Long-Term Outcome of Stroke: Results from a Population-Based Study of Stroke among 450,229 Urban Citizens

Introduction: Little is known regarding long-term stroke outcomes in patients with substance use disorder (SUD). Based on anecdotal data, some individuals use illicit drugs, particularly opioids, in an attempt to reduce stroke mortality, disability, or recurrence. This study is aimed to assess the effect of SUD on stroke outcomes. Methods: Patients were recruited from the Mashhad Stroke Incidence Study, a population-based study of stroke in Iran. For a period of 1 year, all patients with first-ever stroke (FES) were recruited and then followed up for the next 5 years. Disability and functional dependency were defined using modified Rankin Scale (>2) and Barthel Index (<60), respectively. We compared the cumulative rates of mortality in follow-up points using the log-rank test. We used multivariable logistic, Cox regression and competing risk models to assess adjusted hazard ratio (aHR) with 95% confidence interval (CI) of stroke disability, functional dependency, mortality, and recurrence among those with a history of SUD. Results: 595 FES patients (mean age of 64.6 ± 14.8 years) were recruited in this study. Eighty-one (13.6%) were current substance users, including opium (n = 68), naswar (n = 5, 6.1%), hashish (n = 1), heroin (n = 1), and (n = 7) others. The frequency of vascular risk factors was similar between the SUD and non-SUD groups, except for a higher rate of cigarette smoking in the SUD group (p < 0.001). After adjusting for various sociodemographic variables, vascular risk factors, and the severity of stroke at admission, SUD increased the 3-month (aHR: 1.60, CI: 1.01–2.49), 1-year (aHR: 1.73, CI: 1.20–2.65), and 5-year (aHR: 1.72, CI: 1.23–2.35) poststroke mortality risk. We did not observe a significant change in the risk of stroke recurrence, disability, and functional dependency in those with a history of SUD. Conclusion: SUD increased the hazard ratio of stroke mortality with no effect on the disability rate. The public should be advised about the potential harm of substance abuse.
On the estimate of music appraisal from surface EEG: a dynamic-network approach based on cross-sensor PAC measurements

Objective. The aesthetic evaluation of music is strongly dependent on the listener and reflects manifold brain processes that go well beyond the perception of incident sound. Being a high-level cognitive reaction, it is difficult to predict merely from the acoustic features of the audio signal and this poses serious challenges to contemporary music recommendation systems. We attempted to decode music appraisal from brain activity, recorded via wearable EEG, during music listening. Approach. To comply with the dynamic nature of music stimuli, cross-frequency coupling measurements were employed in a time-evolving manner to capture the evolving interactions between distinct brain-rhythms during music listening. Brain response to music was first represented as a continuous flow of functional couplings referring to both regional and inter-regional brain dynamics and then modelled as an ensemble of time-varying (sub)networks. Dynamic graph centrality measures were derived, next, as the final feature-engineering step and, lastly, a support-vector machine was trained to decode the subjective music appraisal. A carefully designed experimental paradigm provided the labeled brain signals. Main results. Using data from 20 subjects, dynamic programming to tailor the decoder to each subject individually and cross-validation, we demonstrated highly satisfactory performance (MAE= 0.948, R 2= 0.63) that can be attributed, mostly, to interactions of left frontal gamma rhythm. In addition, our music-appraisal decoder was also employed in a part of the DEAP dataset with similar success. Finally, even a generic version of the decoder (common for all subjects) was found to perform sufficiently. Significance. A novel brain signal decoding scheme was introduced and validated empirically on suitable experimental data. It requires simple operations and leaves room for real-time implementation. Both the code and the experimental data are publicly available.
Ship detection from scratch in Synthetic Aperture Radar (SAR) images

ABSTRACT Ship detection in Synthetic Aperture Radar (SAR) images has always been a hot topic for research. The development of Deep Neural Networks (DNNs) has strongly promoted the development of computer vision. DNNs are also increasingly applied to SAR ship detection. However, SAR ship detection still faces the following problems: (i) The network used for detection needs to be pre-trained on ImageNet, but there is a large bias between SAR images and ImageNet, which leads to training bias. (ii) The sizes of ship targets vary greatly, and many DNNs do not perform well on multi-scale and small-size SAR ship detection. Therefore, we have designed a SAR ship detector that does not require pre-training. We use DetNet as the backbone network, adopting stacked convolution instead of down-sampling to solve the problem of small object detection and adopt a feature reuse strategy to improve parameter efficiency. In addition, we introduce several branches in the proposal sub-network to provide multi-scale object detection. In the detection sub-network, we use position-sensitive region of interest pooling to improve the prediction accuracy. Experiments on SAR ship dataset prove that our method performs better than some pre-trained networks for small ship detection and complex background ship detection.
Does Rottlerin or its congeners have a potential as adjuvant to antidote therapy of spider/snake venom intoxication?

Rottlerin is a polyphenol natural product isolated from the tree Mallotus philippensis. Rottlerin displays a complex spectrum of pharmacology. Rottlerin has been shown to be an uncoupler of mitochondrialoxidative phosphorylation. It is also known to inhibit the NFAT-5/TonEBP expression. Apart from this it also inhibits thephospholipas A 2 ( PLA2) and phospholipase D enzyme (PLD). Both these enzymes are found in several venoms of the snakes and spider like the brown spider. Rottlerin has also neuroprotective and cardioprotective properties and thus is an ideal candidate for trial in the anti-dote therapy against the deleterious toxic effects of these venoms in humans. This article discusses the hypothetical possibility of its usage in toxic envenomation. Key words: Rottlerin, Phospholipase A2, Phospholipase D, TonEBP, Venom, toxic
Effects of Grazing on the Grassland Vegetation Community Characteristics in Inner Mongolia

Abstract: The continuous increase of livestock production in Inner Mongolia has caused severe degradation of the grassland ecosystems in recent years. Previous grazing experiments have shown a wide range of vegetation responses between the biome types on a global scale, but there is still a lack of sufficient studies to discern the relative responses of a given biome type. We conducted a meta-analysis of vegetation coverage (VC), plant density (PD), total biomass (TB), above-ground biomass (AGB), under-ground biomass (UGB) and Shannon–Weaver Index (SI) in different grassland types in Inner Mongolia obtained under conditions of different grazing intensities and durations. The results showed that grazing decreased VC, TB, AGB, UGB, and PD significantly. Compared to the global and national average values, the negative effects of grazing to steppe biomass in Inner Mongolia were higher than that on the global scale, while less pronounced than that in China. TB of the meadow steppe in Inner Mongolia increased by 40% under moderate grazing intensity and duration because of compensatory growth. SI of the desert and meadow steppe showed negative linear relationships with the grazing intensity in Inner Mongolia. The percentage changes in AGB, PD, and SI to grazing showed quadratic relationships with the mean annual temperature of the experimental year. With increasing mean annual precipitation, the negative effects of grazing on UGB and SI first decreased and then increased, with that of VC and grazing showing a cubic relationship.
A LIKELY INTERSECTIONAL HYBRID IN VACCINIUM (ERICACEAE) ON SAN BRUNO MOUNTAIN, SAN MATEO COUNTY, CALIFORNIA

Abstract A population of Vaccinium from San Bruno Mountain in San Mateo County, California has been confused with V. cespitosum Michx. (V. sect. Myrtillus), a species documented from the mountain, since its initial collection in 1961. These plants resemble V. cespitosum and other species in V. sect. Myrtillus in several characters, but differ most notably in their evergreen habit and well developed calyx lobes. The latter characters are shared by V. ovatum Pursh (V. sect. Pyxothamnus), the only other species of Vaccinium known from San Bruno Mountain, suggesting that the population is a hybrid between V. cespitosum and V. ovatum. We used data from gross morphology, leaf anatomy, and DNA sequences from the nuclear ribosomal internal transcribed spacer (ITS) and plastid matK and ndhF regions to test the hybrid status of this population. Hybrid status is supported by: 1) a plastid sequence profile identical to that of V. cespitosum from San Bruno Mountain and different from the profiles of all other Vaccinium samples in the study, 2) an ITS profile that differs from V. cespitosum from the mountain by only two polymorphic sites, and 3) the possession of gross morphological and anatomical characters that are either shared with one putative parent or the other, or are intermediate between them. Although ITS variation was non-additive and closely matched one of the putative parents, uniparental inheritance has been observed in other hybrids. Two novel gross morphological characters were also observed in the hybrid. The study documents a case of likely intersectional hybridization within Vaccinium, only rarely observed under natural conditions. Vaccinium cespitosum may have been outcompeted by the hybrid to the point of its extirpation from San Bruno Mountain. The population is here newly described as the nothospecies Vaccinium ×brunoense P.W. Fritsch, occurring in an area of only ca. 200 m2 with the number of individuals remaining uncertain because of clonal growth.
Relationship between a diffusion model and a transport model for softwood drying

Transfer equations in a transport model for wood drying are rearranged to give equations that have similar form to those of Luikov's drying model. In this way the transport coefficients in these equations are derived from the transport model and are functions of moisture content, temperature, wood properties (density, permeability, and bound water diffusivity), and properties of moisture (liquid density and viscosity, vapor density, and viscosity). The above-derived equations are used to examine the relationship between the transport model and the simple diffusion model. It is shown that the diffusion model can be regarded as a simplified form of the transport model when the effect of temperature gradient is neglected. The diffusion model is applicable to cases where the temperature gradient is flat or the coefficient for the temperature gradient is small compared to that for the moisture content gradient. In other cases, or where the wood temperature is of concern, the transport drying model should be employed.
GazeWheels: Recommendations for using wheel widgets for feedback during dwell-time gaze input

Abstract We present GazeWheels: a series of visual feedback methods for dwell-based gaze input in the form of a wheel that is filled gradually until target selection. We evaluate three variations: Resetting, Pause & Resume and Infinite GazeWheel, and study how dwell duration and visual feedback position (co-located vs remote) impact performance. Findings from a user study (N = 19) show that Infinite and Pause & Resume GazeWheels are error prone but significantly faster than Resetting GazeWheel even when including error correction time. We conclude with five design recommendations.
Nanopore Dwell Time Analysis Permits Sequencing and Conformational Assignment of Pseudouridine in SARS-CoV-2

Nanopore devices can directly sequence RNA, and the method has the potential to determine locations of epitranscriptomic modifications that have grown in significance because of their roles in cell regulation and stress response. Pseudouridine (Ψ), the most common modification in RNA, was sequenced with a nanopore system using a protein sensor with a helicase brake in synthetic RNAs with 100% modification at 18 known human pseudouridinylation sites. The new signals were compared to native uridine (U) control strands to characterize base calling and associated errors as well as ion current and dwell time changes. The data point to strong sequence context effects in which Ψ can easily be detected in some contexts while in others Ψ yields signals similar to U that would be false negatives in an unknown sample. We identified that the passage of Ψ through the helicase brake slowed the translocation kinetics compared to U and showed a smaller sequence bias that could permit detection of this modification in RNA. The unique signals from Ψ relative to U are proposed to reflect the syn-anti conformational flexibility of Ψ not found in U, and the difference in π stacking between these bases. This observation permitted analysis of SARS-CoV-2 nanopore sequencing data to identify five conserved Ψ sites on the 3’ end of the viral sub-genomic RNAs, and other less conserved Ψ sites. Using the helicase as a sensor protein in nanopore sequencing experiments enables detection of this modification in a greater number of relevant sequence contexts. The data are discussed concerning their analytical and biological significance.
Knowledge production and translation of science between Academia and Industry: assessing the impact of R&D in India

India is at the 3rd position worldwide in terms of publication of scientific literature. However, in terms of productivity, it has been consistently failing to transform the research knowledge into industrial output. This study compares India with the leading countries to understand its lacuna in terms of R&D policy and outputs. Although scientific publications are regarded as the output of basic research, patent applications serve as a better indicator of the applied research. This paper assesses the important determinants for patent filings of a nation. It also focuses on the role of academia and industry collaboration in R&D and the productivity of a nation. We found that the higher the GERD (total Gross Domestic Expenditure of R&D) and the R&D personnel in a nation, the higher the patent filings of the nation. Moreover, we show that academia-industrial collaboration plays a key role in transforming basic research into real-world applications, as we illustrate the government’s role in making necessary policies to make the collaboration successful. This paper highlights the significance of investing in R&D to improve the productivity of a nation, as also the need to design policies to strengthen the applied research environment by fostering solution-centric collaborations between academia and industry.
Generative perspective of the primary visual cortex

According to analysis-by-synthesis theories of perception, the primary visual cortex (V1) reconstructs visual stimuli through top-down pathway, and higher-order cortex reconstructs V1 activity. Experiments also found that neural representations are generated in a topdown cascade during visual imagination. What code does V1 provide higher-order cortex to reconstruct or simulate to improve perception or imaginative creativity? What unsupervised learning principles shape V1 for reconstructing stimuli so that V1 activity eigenspectrum is power-law with close-to-1 exponent? Using computational models, we reveal that reconstructing the activities of V1 complex cells facilitate higher-order cortex to form representations smooth to shape morphing of stimuli, improving perception and creativity. Power-law eigenspectrum with close-to-1 exponent results from the constraints of sparseness and temporal slowness when V1 is reconstructing stimuli, at a sparseness strength that best whitens V1 code and makes the exponent most insensitive to slowness strength. Our results provide fresh insights into V1 computation.
A broadly active fucosyltransferase LmjFUT1 whose mitochondrial localization and catalytic activity is essential in the parasitic protozoan Leishmania

Glycoconjugates play major roles in the infectious cycle of the trypanosomatid parasite Leishmania. While GDP-Fucose synthesis is essential (Guo et al 2017), fucosylated glycoconjugates have not been reported in Leishmania major. Four predicted fucosyltransferases appear conventionally targeted to the secretory pathway; SCA1/2 play a role in side-chain modifications of lipophosphoglycan, while gene deletion studies here showed that FUT2 and SCAL were not essential. Unlike most eukaryotic glycosyltransferases, the predicted α 1-2 fucosyltransferase encoded by FUT1 localized to the mitochondrion. A quantitative ‘plasmid segregation’ assay, expressing FUT1 from the multicopy episomal pXNG vector in a chromosomal null Δfut1- background, established that FUT1 is essential. Similarly “plasmid shuffling” confirmed that both enzymatic activity and mitochondrial localization were required for viability, comparing import-blocked or catalytically inactive enzymes respectively. Enzymatic assays of tagged proteins expressed in vivo or of purified recombinant FUT1 showed it had a broad fucosyltransferase activity including glycan and peptide substrates. Unexpectedly a single rare Δfut1-s segregant (Δfut1s) was obtained in rich media, which showed severe growth defects accompanied by mitochondrial dysfunction and loss, all of which were restored upon FUT1 re-expression. Thus, FUT1 along with the similar Trypanosoma brucei enzyme TbFUT1 (Bandini et al 2021) joins the eukaryotic O-GlcNAc transferase isoform as one of the few glycosyltransferases acting within the mitochondrion. Trypanosomatid mitochondrial FUT1s may offer a facile system for probing mitochondrial glycosylation in a simple setting and their essentiality renders it an attractive target for chemotherapy of these serious human pathogens. Significance Statement Abundant surface glycoconjugates play key roles in the infectious cycle of protozoan parasites including Leishmania. Through defining biosynthetic pathways we identified a fucosyltransferase FUT1 that was localized to the parasite mitochondrion, an atypical compartment for glycosyltransferases. FUT1 was essential for normal growth, requiring both mitochondrial localization and enzymatic activity. Loss of FUT1 in a unique segregant showed extensive mitochondrial defects. Enzymatic tests showed FUT1 could fucosylate glycan and peptide substrates in vitro, although as yet the native substrate is unknown. Trypanosomatid mitochondrial FUT1s may offer a facile system in the future for probing mitochondrial glycosylation in a setting uncomplicated by multiple isoforms targeted to diverse compartments, and its essentiality renders it an attractive target for chemotherapy of these deadly parasites.
Information transfer in mammalian glycan-based communication

Glycan-binding proteins, so-called lectins, are exposed on mammalian cell surfaces and decipher the information encoded within glycans translating it into biochemical signal transduction pathways in the cell. These glycan-lectin communication pathways are complex and difficult to analyse. However, quantitative data with single cell resolution provides means to disentangle the associated signalling cascades. We chose C-Type lectin receptors (CLRs) expressed on immune cells as a model system to study their capacity to transmit information encoded in glycans of incoming particles. Lectin receptor NFκB-reporter cell lines expressing DC-SIGN, MCL, dectin-1, dectin-2, and mincle, as well as TNFαR and TLR-1&2 in monocytic cell lines were characterized by comparing their efficiency to transmit glycan-encoded information. The information content was measured by following NFκB dependent GFP expression. While most receptors did transmit information to NFκB efficiently, we found dectin-2 to be an inefficient signalling receptor. Yet upon closer analysis we show that the sensitivity of the dectin-2 signal transduction pathway (EC50) can be enhanced by overexpression of its co-receptor FcRγ, while its transmitted information cannot. In this context, we expanded our investigation towards the integration of multiple signal transduction pathways, which is crucial during pathogen recognition. We show how lectin receptors using a similar signal transduction pathway (dectin-1 and dectin-2) are being integrated; by striking a compromise between the lectins. By using dectin-2 and other lectins as example we demonstrate how cellular heterogeneity and the receptor itself determine the efficiency and therefore outcome of the signal transduction pathways.