Miracle-dz/UniFAR-FTUs huggingface dataset:
The PSF·p54nrb Complex Is a Novel Mnk Substrate That Binds the mRNA for Tumor Necrosis Factor α*

To identify new potential substrates for the MAP kinase signal-integrating kinases (Mnks), we employed a proteomic approach. The Mnks are targeted to the translational machinery through their interaction with the cap-binding initiation factor complex. We tested whether proteins retained on cap resin were substrates for the Mnks in vitro, and identified one such protein as PSF (the PTB (polypyrimidine tract-binding protein)-associated splicing factor). Mnks phosphorylate PSF at two sites in vitro, and our data show that PSF is an Mnk substrate in vivo. We also demonstrate that PSF, together with its partner, p54nrb, binds RNAs that contain AU-rich elements (AREs), such as those for proinflammatory cytokines (e.g. tumor necrosis factor α (TNFα)). Indeed, PSF associates specifically with the TNFα mRNA in living cells. PSF is phosphorylated at two sites by the Mnks. Our data show that Mnk-mediated phosphorylation increases the binding of PSF to the TNFα mRNA in living cells. These findings identify a novel Mnk substrate. They also suggest that the Mnk-catalyzed phosphorylation of PSF may regulate the fate of specific mRNAs by modulating their binding to PSF·p54nrb.
Off-Label Thrombolysis for Acute Ischemic Stroke: Rate, Clinical Outcome and Safety Are Influenced by the Definition of ‘Minor Stroke’

Background: Several contraindications for intravenous thrombolysis are not based on controlled trials. Specialized stroke centers often apply less restrictive criteria. The aim of our study was to analyze how many patients at our institution receive off-label thrombolysis. In addition, clinical outcome and safety data were compared to those from patients treated on-label, and the influence of different definitions of ‘minor stroke’ were examined. Methods: Consecutive thrombolysis patients treated between January 2006 and January 2010 were included. Patients treated off-label were compared to patients given on-label therapy according to the European license. Since no specified definition for ‘minor neurological deficit’ exists in the license, two distinct definitions were considered off-label, i.e. National Institutes of Health Stroke Scale score (NIHSSS) <1 (definition 1) and NIHSSS ≤4 (definition 2). Results: Of a total of 422 patients, 232 (55%) were treated off-label. The most prevalent off-label criteria (OLCs) were the following: age >80 years (n = 113), minor stroke (definition 1, n = 3; definition 2, n = 84), elevated blood pressure necessitating aggressive treatment (n = 75), time window >3 h (n = 71) and major surgery or trauma within the preceding 3 months (n = 20). In group comparisons, off-label patients had an overall worse outcome using definition 1 for minor stroke, while there was no difference when definition 2 was applied. In multivariate analysis, off-label therapy (definition 1) in general and age >80 years were independent predictors of poor outcome. None of the contraindications were associated with an increased bleeding risk. Conclusions: Off-label therapy is frequently applied at our center and is not associated with higher complication rates. Overall outcome of off-label treatment largely depends on the definition used for minor stroke. Besides age >80 years, a known poor prognostic factor, no other specific OLC was associated with poor outcome. Our data suggest that the criteria in the European license may be too restrictive.
GRB 140606B/iPTF14bfu: Detection of shock-breakout emission from a cosmological γ -ray burst?

We present optical and near-infrared photometry of GRB 140606B (z = 0.384), and optical photometry and spectroscopy of its associated supernova (SN). The results of our modelling indicate that the bolometric properties of the SN (M_(Ni) = 0.4 ± 0.2 M_⊙, M_(ej) = 5 ± 2 M_⊙, and E_K = 2 ± 1 × 10^(52) erg) are fully consistent with the statistical averages determined for other γ-ray burst (GRB)-SNe. However, in terms of its γ-ray emission, GRB 140606B is an outlier of the Amati relation, and occupies the same region as low luminosity (ll) and short GRBs. The γ-ray emission in llGRBs is thought to arise in some or all events from a shock breakout (SBO), rather than from a jet. The measured peak photon energy (E_p ≈ 800 keV) is close to that expected for γ-rays created by an SBO (≳ 1 MeV). Moreover, based on its position in the M_(V, p)-L_(iso, γ) plane and the E_K–Γβ plane, GRB 140606B has properties similar to both SBO-GRBs and jetted-GRBs. Additionally, we searched for correlations between the isotropic γ-ray emission and the bolometric properties of a sample of GRB-SNe, finding that no statistically significant correlation is present. The average kinetic energy of the sample is E¯_K = 2.1×10^(52) erg. All of the GRB-SNe in our sample, with the exception of SN 2006aj, are within this range, which has implications for the total energy budget available to power both the relativistic and non-relativistic components in a GRB-SN event.
MicroRNA 181 Suppresses Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection by Targeting PRRSV Receptor CD163

ABSTRACT We previously showed that microRNA 181 (miR-181) can inhibit PRRSV replication by directly targeting its genomic RNA. Here, we report that miR-181 can downregulate the PRRSV receptor CD163 in blood monocytes and porcine alveolar macrophages (PAMs) through targeting the 3′ untranslated region (UTR) of CD163 mRNA. Downregulation of CD163 leads to the inhibition of PRRSV entry into PAMs and subsequently suppresses PRRSV infection. Our findings indicate that delivery of miR-181 can be used as antiviral therapy against PRRSV infection.
Peptide-mediated Interference of TIR Domain Dimerization in MyD88 Inhibits Interleukin-1-dependent Activation of NF-κB*

Myeloid differentiation factor 88 (MyD88) plays a crucial role in the signaling pathways triggered by interleukin (IL)-1 and Toll-like receptors in several steps of innate host defense. A crucial event in this signaling pathway is represented by dimerization of MyD88, which allows the recruitment of downstream kinases like IRAK-1 and IRAK-4. Herein, we have investigated the function of the Toll/IL-1 receptor (TIR) domain in MyD88 homodimerization in cell-free and in vitro experimental settings by using epta-peptides that mimic the BB-loop region of the conserved TIR domain of different proteins. By using a pull-down assay with purified glutathione S-transferase-MyD88 TIR or co-immunoprecipitation experiments, we found that epta-peptides derived from the TIR domain of MyD88 and IL-18R are the most effective in inhibiting homodimerization with either the isolated TIR or full-length MyD88. Moreover, we demonstrated that a cell permeable analog of MyD88 epta-peptide inhibits homodimerization of MyD88 TIR domains in an in vitro cell system and significantly reduces IL-1 signaling, as assayed by activation of the downstream transcription factor NF-κB. Our results indicate that the BB-loop in TIR domain of MyD88 is a good target for specific inhibition of MyD88-mediated signaling in vivo.
Synergism between prostaglandin E2 and isoproterenol in stimulating glucose oxidation in the heart.

The increase in cardiac contractile function following adrenergic stimulation is accompanied by increased glucose metabolism. Since prostaglandin E2 (PGE2) can internalize beta-receptors, we determined what effects PGE2 and isoproterenol have on glycolysis and glucose oxidation in the isolated working rat heart. All hearts were perfused with Krebs-Henseleit buffer containing 11 mM [5-3H, 14C(U)]glucose, 100 microU/mL insulin, and 3% albumin. In the presence of 0.4 mM palmitate and 1.25 mM free Ca2+, isoproterenol (3 x 10(-8) M) increased the heart rate x peak systolic pressure product from 27 +/- 1 to 43 +/- 1 mmHg.beats. min-1.10(3) (1 mmHg = 133.3 Pa). This was accompanied by an increase in glycolytic rates from 3564 +/- 231 to 7775 +/- 475 nmol.g-1 dry weight min-1 and an increase in glucose oxidation from 930 +/- 72 to 2591 +/- 239 nmol.g-1 dry weight.min-1. Addition of PGE2 (10(-9) M) did not affect the isoproterenol stimulation of glycolysis, but caused a further increase in glucose oxidation (to 3863 +/- 495 nmol.g-1 dry weight.min-1). In the absence of isoproterenol, 10(-9) M PGE2 had no effect on either glycolysis or glucose oxidation. When perfusate [Ca2+] was raised to 2.5 mM, a significant increase in glycolysis was seen in control hearts (from 3564 +/- 231 to 5679 +/- 374 nmol.g-1 dry weight.min-1). The effects of isoproterenol and PGE2 on glucose metabolism remained, although the synergistic effects of PGE2 on glucose oxidation were less dramatic. When 1.2 mM palmitate was present in hearts perfused with 2.5 mM Ca2+, a decrease was seen in both glycolysis (from 5679 +/- 374 to 3027 +/- 346 nmol.g-1 dry weight.min-1) and glucose oxidation (from 1056 +/- 170 to 221 +/- 29 nmol.g-1 dry weight.min-1). Even at this high concentration of fatty acid, isoproterenol stimulated glucose oxidation (from 221 +/- 29 to 859 +/- 69 nmol.g-1 dry weight min-1), and addition of PGE2 resulted in a significant further increase (1021 +/- 139 nmol.g-1 dry weight.min-1). These data demonstrate that concentrations of PGE2 that bind to the high affinity cardiac PGE2 receptor have no effect on glucose metabolism in the absence of beta-agonists. In the presence of isoproterenol, which dramatically stimulates both glycolysis and glucose oxidation, PGE2 has a synergistic effect on glucose oxidation at lower fatty acid concentrations. These findings suggest that PGE2 receptors in the heart function to potentiate rather than decrease beta-adrenergic stimulation of glucose metabolism.
Live working on electrode line of ± 800kV DC transmission project

It is necessary to research live working on UHV electrode line, so as to support DC UHV maintenance. As the electrode line of Zhengzhou converter station for example, the ground faults overvoltage on BP and GR modes were calculated. The maximum switching overvoltage was 288kV under the condition of live working. It was determined by the grounding fault of high end YY converter valves. The switching impulse flashover was carried in the live working air gaps of simulation tower. The results showed that the discharge voltages between worker's back and tower were lowest, and Discharge voltage of arcing horn was obviously higher than that between equipotent personnel and tower. The live working safety distance was calculated in usage. As the safety margin is 1.2, the safety distance is 0.65m. The electric field strength of live workers is less than 240kV/m, so the workers do not need to protect the electric field. And the maximum operating temperature of the conductor is less than 120 V, live working worker must avoid direct contact with high temperature wire, should use insulation tools or wear thermal insulation protective tools. Barehand method, insulating method and gloving method can be charged for electrode line. Above-mentioned results are available for reference to live working carried on other electrode lines of 800kV DC transmission project.
A 90-nm CMOS Broadband and Miniature Q-band Balanced Medium Power Amplifier

This paper presents a Q-band balanced medium power amplifier fabricated using standard 90-nm 1P9M CMOS technology. The balanced amplifier, which is constructed with two broadband amplifiers and two broadside couplers using thin-film microstrip (TFMS) line technique, has a compact chip size of 0.78 x 0.92 mm2. The MMIC demonstrates a measured gain of 14.5 dB at 48 GHz. With the feature of the balanced amplifier, the MMIC has a 3-dB bandwidth up to 37.2 % from 35 to 51 GHz with flat gain and return loss frequency response. Furthermore, the balanced amplifier delivers a saturation output power of 10.6 dBm with 8% PAE and OPldB is 7.5 dBm.
Da modelagem de plantas a dinamica de multidões : um modelo de animação comportamental bio-inspirado

Este trabalho apresenta um metodo para simulacao de multidoes baseado no algoritmo de colonizacao do espaco. Este algoritmo foi originalmente proposto para modelar padroes de nervuras em folhas vegetais e de ramificacoes em arvores. A tecnica baseia-se na competicao por espaco entre nervuras ou ramificacoes durante o crescimento vegetal. Adaptado a simulacao de multidoes, o algoritmo de colonizacao do espaco visa simular a competicao por espaco durante o movimento dos pedestres. Varios comportamentos observados em multidoes reais, tais como evitar colisoes, variar a velocidade de deslocamento do pedestre em funcao da densidade populacional e formar vias (lanes) de pedestres, nas quais o pedestre seguira aquele imediatamente a sua frente, cuja direcao e sentido sao similares, sao propriedades do algoritmo. O modelo de simulacao de multidoes proposto tambem caracteriza-se pela simplicidade de implementacao, robustez e eficencia computacional, permitindo, de acordo com o ambiente de simulacao adotado, o controle interativo da multidao simulada Abstract
Mécanismes neuronaux sous-tendant l'apprentissage perceptif olfactif chez la souris adulte

Le bulbe olfactif est le siege d'une neurogenese adulte pouvant jouer un role dans les apprentissages olfactifs. Nous nous sommes interesses a son role dans un type d'apprentissage olfactif : l'apprentissage perceptif. Tout d'abord, l'enrichissement permet d'ameliorer la discrimination d'odeurs proches : c'est l'apprentissage perceptif. En etudiant les effets de la suppression de la neurogenese pendant l'enrichissement nous avons montre que la neurogenese etait necessaire a l'apprentissage perceptif en modulant l'inhibition des cellules mitrales par les cellules granulaires. Ensuite, nous avons etudie le role de la noradrenaline dans cet apprentissage. La noradrenaline est necessaire a l'apprentissage perceptif et suffisante pour en mimer les effets. De plus, nous avons montre que la neurogenese bulbaire etait essentielle a l'action de la noradrenaline pour permettre l'apprentissage perceptif. Enfin, nous nous sommes interesses a l'effet du vieillissement sur l'apprentissage perceptif. Nous avons trouve qu'il existait un defaut d'apprentissage perceptif chez la souris âgee en lien avec une diminution de la neurogenese. En revanche, une stimulation noradrenergique permet de restaurer l'apprentissage perceptif sans modulation de la neurogenese bulbaire suggerant l'existence de mecanismes compensatoires. L'apprentissage perceptif est sous-tendu par la neurogenese bulbaire, via le systeme noradrenergique, pour permettre une hausse d'inhibition des cellules mitrales par les cellules granulaires ameliorant la discrimination des odeurs proches. Avec l'âge, l'apprentissage perceptif peut etre restaure suggerant une plasticite toujours presente dans un systeme olfactif vieillissant
Manipulation of the spatial grazing behaviour of cattle in extensive and mountainous rangelands

Spatial behaviour of livestock is a critical factor in rangeland management. Ongoing research suggests that new approaches can be used to manipulate where beef cattle graze in extensive or rugged rangeland pastures. The combination of strategic supplement placement and low-stress herding can be used to target cattle grazing and potentially may be useful for managing fine fuels. A phenotype to genotype association study of cattle grazing distribution suggests that use of rugged terrain and areas far from water is inherited. Although more research is needed, selection for animals specifically adapted for mountainous terrain or extensive pastures may be an option for improving grazing management in the near future.
A prospective randomized, double-blind, controlled clinical trial comparing epidural butorphanol plus corticosteroid with corticosteroid alone for sciatica due to herniated nucleus pulposus

Objective: To compare the efficacy of up to 3 epidural butorphanol plus corticosteroid with corticosteroid alone for sciatica due to herniated nucleus pulposus. Materials and Methods: In a randomized, double-blind controlled clinical trial, we administered up to 3 epidural injections of either 80 mg (2 mL) of methylprednisolone acetate and 1 mg (1 mL) of butorphanol diluted with 7 mL of isotonic saline or 80 mg (2 mL) of methylprednisolone acetate diluted with 8 mL of isotonic saline by a lumbar interlaminar approach under fluoroscopic guidance to 120 patients (60 patients in each group) with sciatica due to a herniated nucleus pulposus lasting for 4 weeks to 1 year. All patients had scores higher than 30 mm on visual analog scale (VAS). Information on the use of paracetamol, intensity of pain on a VAS ranging from 0 (no pain) to 100 mm (worst pain possible), Schober's test (cm), Straight Leg Raising test, neurologic examination assessing sensory deficits, motor deficits and reflex changes, and Oswestry Low Back Pain Disability Questionnaire were evaluated at 3 weeks, 6 weeks, and 3 months after the first injection. Results: There were no significant differences between the 2 groups with regard to baseline characteristics, withdrawals, and complication rate. Three weeks, 6 weeks, and 3 months after the first injection, all the outcome measures in the butorphanol plus corticosteroid group were significantly different from that of the corticosteroid group. Conclusions: Epidural butorphanol plus corticosteroid injections, as compared with corticosteroid alone injections, offered marked improvement in pain, reflex, motor and sensory deficits, and functional status and reduced the need for analgesics. Level of Evidence: Therapeutic Level I.
Strategic alignment: a practitioner's perspective

Purpose – Organizations are becoming increasingly aware of the importance of aligning information systems with organizational processes, goals and strategies. One way of representing and analysing strategic alignment is through the creation of a causal‐loop diagram, a subject which this paper seeks to examine.Design/methodology/approach – The exploratory research presented here involved six senior IS/IT managers during three two‐hour focus group sessions, which led to the development of such a diagram. The focus group sessions were recorded, transcribed and analysed using content analysis.Findings – The diagram presents a systemic view of IS/business alignment within organizations, as seen through the lens of these practitioners. The research suggests that, although practitioners understand that a high level of connection between IS and business planning processes may be dependent on the level of integration between the IS group and other sections of the organization, they are still unable to develop the .
Implications of 15N‐metabolic labeling for automated peptide identification in Arabidopsis thaliana

We report the first metabolic labeling of Arabidopsis thaliana for proteomic investigation, demonstrating efficient and complete labeling of intact plants. Using a reversed‐database strategy, we evaluate the performance of the MASCOT search engine in the analysis of combined natural abundance and 15N‐labeled samples. We find that 15N‐metabolic labeling appears to increase the ambiguity associated with peptide identifications due in part to changes in the number of isobaric amino acids when the isotopic label is introduced. This is reflected by changes in the distributions of false positive identifications with respect to MASCOT score. However, by determining the nitrogen count from each pair of labeled and unlabeled peptides we may improve our confidence in both heavy and light identifications.
Finding Oneself in the Other

This is the second of three volumes of posthumously collected writings of G. A. Cohen, who was one of the leading, and most progressive, figures in contemporary political philosophy. This volume brings together some of Cohen's most personal philosophical and nonphilosophical essays, many of them previously unpublished. Rich in first-person narration, insight, and humor, these pieces vividly demonstrate why Thomas Nagel described Cohen as a "wonderful raconteur. " The nonphilosophical highlight of the book is Cohen's remarkable account of his first trip to India, which includes unforgettable vignettes of encounters with strangers and reflections on poverty and begging. Other biographical pieces include his valedictory lecture at Oxford, in which he describes his philosophical development and offers his impressions of other philosophers, and "Isaiah's Marx, and Mine," a tribute to his mentor Isaiah Berlin. Other essays address such topics as the truth in "small-c conservatism," who can and can't condemn terrorists, and the essence of bullshit. A recurring theme is finding completion in relation to the world of other human beings. Engaging, perceptive, and empathetic, these writings reveal a more personal side of one of the most influential philosophers of our time.
The X/Open Internationalization Model

Software internationalization standards allow developers to create applications that are neutral with respect to language and cultural information. X/Open adopted a model for internationalization and has revised the model several times to expand the range of support. The latest version of the X/Open internationalization model, which supports multibyte code sets, provides a set of interfaces that enables users in most of Europe and Asia to develop portable applications independent of the language and code set. One implementation of this model, the internationalized DEC OSF/1 AXP version 1.2 (based on OSF/1 release 1.2) supports complex Asian languages such as Chinese and Japanese.
Algunos problemas de control y diseño óptimo en sistemas gobernados por ecuaciones diferenciales parciales

Este articulo contiene una breve descripcion de cuatro problemas diferentes relacionadas con el control y el Diseno Optimo en sistemas gobernados por Ecuaciones Diferenciales Parciales. El primer problema que se presenta consiste en el analisis y control de sistemas hibridos compuestos por cuerdas y vigas acopladas con masas de Dirac. El segundo problema se refiere al estudio de la propagacion de ondas en medios muy heterogeneos. El tercer problema es una adaptacion del metodo de la homogeneizacion al disen optimo de un conocido problema en ingenieria nuclear. Por ultimo, describimos una forma optima de estabilizar las vibraciones de una cuerda vibrante. Palabras clave: Control, Sistemas Hibridos, Homogeneizacion, Diseno Optimo Clasificacion por materias AMS: 35B37, 35B27, 35L05, 35L30, 35P15
PARTÍCULA MODAL VS. PARTICULA EXPRESIVA: CONTRIBUCIÓN AL ESTUDIO DE TIPOLOGÍA PARTICULAR Sobre el material de la lengua rusa

RESUMEN. Las particulas son unidades linguisticas muy complejas, ya que pueden desempenar distintos valores semanticos, funciones sintacticas, objetivos perlocutivos, etc. Las particulas pueden englobarse dentro de las palabras auxiliares, pero debido a su caracter complejo habra que partir de la funcion que desempena una particula dada para poder realizar una clasificacion exacta y establecer los tipos de relaciones entre diferentes particulas. Es asi como en nuestro trabajo llegamos a la conclusion de que la nocion de particula modal debe diferenciarse de la de particula expresiva (no seria una hiperonimo de otra) debido a las diferencias entre los conceptos mismos de modalidad y expresividad. PALABRAS CLAVE. Particulas modales, particulas expresivas, modalidad, expresividad.