Miracle-dz/UniFAR-FTUs huggingface dataset:
Cell Death in Retinoblastoma: Electron Microscopic, Immunohistochemical, and DNA Fragmentation Studies

Apparent cell loss by apoptosis occurs in carcinomatous tissue. To investigate cell death in retinoblastoma (Rb), ultrastructural examination, ApopTag staining, electrophoresis to detect apoptotic DNA fragmentation, and flow cytometric studies were performed. Immunostaining for the oncogenic products bcl-2 and p53 was also carried out. Relationships between the proliferation fraction (PF), apoptotic index (AI), and the distribution of bcl-2 and p53 were investigated according to the degree of histologic differentiation of Rb. Ultrastructurally,two patterns of cell death were seen. Necrotic cells exhibited vacuolation of cytoplasmic organelles with a marked lytic change in the karyoplasm and cytoplasm. In contrast, apoptotic cells were characterized by crescentic margination of chromatin, condensation of karyoplasm and cytoplasm, and fragmentation of the nucleus. Differentiated Rb had a low AI value (< 1%),whereas undifferentiated Rb had a high AI value (> 8%). The PF of undifferentiated RB (31%) was significantly higher than that of differentiated RB (14%). Analysis of DNA fragmentation using 3'-end labeling with terminal transferase indicated that undifferentiated Rb has increased DNA cleavage. The distribution of apoptotic bodies within Rb was inversely correlated with the expression of bcl-2. A majority of tumor cells of differentiated Rb were negative for p53, whereas 20-40% of tumor cells of undifferentiated Rb showed a positive reaction for p53. These findings suggest that the degree of susceptibility to apoptosis is closely related to PF, is inversely related to the degree of differentiation of Rb, and is protected by oncogene bcl-2.
Ameloblastoma: current etiopathological concepts and management.

Ameloblastoma is a benign odontogenic tumor of epithelial origin. It is locally aggressive with unlimited growth capacity and has a high potential for malignant transformation as well as metastasis. Ameloblastoma has no established preventive measures although majority of patients are between ages 30 and 60 years. Molecular and genetic factors that promote oncogenic transformation of odontogenic epithelium to ameloblastoma are strongly linked to dysregulation of multiple genes associated with mitogen-activated protein kinase, sonic hedgehog, and WNT/β-catenin signaling pathways. Treatment of ameloblastoma is focused on surgical resection with a wide margin of normal tissue because of its high propensity for locoregional invasion; but this is often associated with significant patient morbidity. The relatively high recurrence rate of ameloblastoma is influenced by the type of molecular etiological factors, the management approach, and how early the patient presents for treatment. It is expected that further elucidation of molecular factors that orchestrate pathogenesis and recurrence of ameloblastoma will lead to new diagnostic markers and targeted drug therapies for ameloblastoma.
Type D personality predicts clinical events after myocardial infarction, above and beyond disease severity and depression.

OBJECTIVE To investigate the effect of Type D personality (high negative affectivity and social inhibition) on cardiac death and/or recurrent myocardial infarction (MI) in patients with acute MI, after adjustment for disease severity and depression. To explore the differential effect of Type D on early (< or = 6 months) versus late (> 6 months) events separately. METHOD Patients hospitalized for acute MI (N = 473) were recruited between May 2003 and May 2006. Patients were assessed on demographic and clinical variables and completed the Type D Personality Scale within the first week of hospital admission for acute MI; depression severity was assessed with the 17-item Hamilton Depression Rating Scale. The mean follow-up period was 1.8 years. RESULTS There were 44 events attributable to cardiac death (n = 16) or recurrent MI (n = 28), with 26 early and 18 late events. Type D patients were at cumulative increased risk of death/recurrent MI compared with non-Type D patients (16.3% vs 7.8%; P = .012). Cardiac history, left ventricular ejection fraction, and use of statins were predictors of total and late death/recurrent MI, with statins showing a substantial protective effect. In addition, cardiac history and use of statins were significantly associated with early death/recurrent MI. Type D patients had a 2-fold increased risk of total death/recurrent MI after adjustment for disease severity and depression (HR = 2.23; 95% CI, 1.14-4.35; P = .019) and a more than 3-fold increased risk of late death/recurrent MI (HR = 3.57; 95% CI, 1.23-10.30; P = .019). CONCLUSIONS Type D was a strong predictor of adverse cardiac outcome after acute MI, above and beyond disease severity and depression severity, and the associated risk was similar to that of traditional cardiovascular risk factors. Type D may be an important psychosocial factor to assess in patients post-MI for risk stratification purposes.
Androgen and estrogen receptor‐β distribution within spinal‐projecting and neurosecretory neurons in the paraventricular nucleus of the male rat

Activation of the hypothalamic–pituitary–adrenal (HPA) axis is initiated by neurosecretory neurons residing within the medial parvicellular part of the hypothalamic paraventricular nucleus (PVN). Despite the potency by which sex steroids operate on HPA and medial parvocellular responses to stress, previous topographic and phenotypic studies suggest that gonadal steroid hormone receptors are scarcely, if at all, expressed by PVN neurons controlling anterior pituitary corticotropes. Guided by the pattern of retrograde accumulation of fluorogold, we used a direct connectional approach to define the distribution of androgen receptors (AR) and estrogen‐beta receptors (ER‐β) within populations of neurosecretory vs. nonneurosecretory neurons in the PVN. Juxtaposition of AR‐immunoreactivity (ir) and ER‐β mRNA to the pattern of intravenous fluorogold labeling showed these steroid hormone receptors to be concentrated within portions of the PVN devoid of neurosecretory neurons. Superimposing receptor profiles onto the pattern of spinal retrograde labeling confirmed a selective distribution of AR‐ir within autonomic‐related cells of the medial parvocellular division, including its dorsal, lateral, and ventral medial components. ER‐β mRNA expression was likewise concentrated within regions accumulating spinal tracer, highest within the ventral aspect of the PVN. These results indicate a direct influence of gonadal hormones on preautonomic effector neurons and remain in keeping with an indirect influence of androgens on adrenocorticotropin‐regulating neurons in the PVN. J. Comp. Neurol. 499:911–923, 2006. © 2006 Wiley‐Liss, Inc.
Immunohistochemical organization of the ventral lateral geniculate nucleus in the ground squirrel

The ventral lateral geniculate nucleus (vLGN) of the thirteen‐lined ground squirrel (Citellus tridecemlineatus) is a highly differentiated nucleus that is divisible into five major subdivisions on the basis of retinal projections and cytoarchitecture. To pursue the likelihood that these subdivisions (the dorsal cap, intergeniculate leaflet, external magnocellular lamina, internal magnocellular lamina, and parvicellular segment) correlate with the functional diversity of this complex, the present study examined the neurochemical composition of the vLGN with regard to substances that have previously proved useful in distinguishing functionally distinct subregions within nuclei (i.e., neuropeptide Y (NPY), substance P (SP), leucine and methionine enkephalins, gamma‐aminobutyric acid (GABA), cytochrome oxidase (CO), acetylcholinesterase (AChE), and NADPH‐diaphorase).
Dose-Response Study of the Analgesic Effect of Lanepitant in Patients with Painful Diabetic Neuropathy

Lanepitant is effective in the formalin analgesic model suggesting efficacy in painful neuropathy. This study was designed to evaluate the dose-response effect of lanepitant in patients with daily moderate to severe, bilateral, distal neuropathic pain. After a 1-to 3-week lead-in period, patients were randomly allocated to double-blind, parallel treatment with lanepitant 50 mg daily (n = 27), 100 mg daily (n = 27), 200 mg twice daily (n = 13), or placebo (n = 26) over 8 weeks. Patients reported average daytime pain and average nighttime pain intensity. Plasma concentrations and amount of adjunctive analgesic medication were obtained at all visits after baseline. Patient global evaluation and clinician global impression were obtained at weeks 3 and 8. Safety was assessed by adverse events, vital signs, laboratory analytes, and electrocardiogram. No dosage of lanepitant differed significantly from placebo. Efficacy did not increase with lanepitant dosage, and higher plasma concentrations were no more effective than lower plasma concentrations. The adverse event diarrhea was more frequent for lanepitant-treated patients. Although well tolerated, lanepitant was ineffective in relieving pain of diabetic neuropathy.
Hepatitis C virus infection in Iceland: a recently introduced bloodborne disease

SUMMARY This study demonstrates a very high prevalence of antibodies to hepatitis C virus among Icelandic intravenous (i.v.) drug users. Of 152 identified i.v. drug users 95 (63%) were shown to have antibodies to the hepatitis C virus. In contrast the seroprevalence in the general Icelandic population is low, (0·2%). Almost all cases of hepatitis C virus infection in Iceland are due to i.v. drug use or to use of infected blood or blood products. Sporadic cases with unexplained modes of transmission, a significant portion of hepatitis C infections elsewhere, are virtually non-existent in Iceland. The results of this study are consistent with the hypothesis that the sporadic community-acquired cases could be caused by blood transfer due to bites from insect vectors such as mosquitoes which are not found in Iceland.
Molecular systematic studies of eubacteria, using sigma70-type sigma factors of group 1 and group 2

Sigma factors of the sigma70 family were used as a phylogenetic tool to compare evolutionary relationships among eubacteria. Several new sigma factor genes were cloned and sequenced to increase the variety of available sequences. Forty-two group 1 sigma factor sequences of various species were analyzed with the help of a distance matrix method to establish a phylogenetic tree. The tree derived by using sigma factors yielded subdivisions, including low-G+C and high-G+C gram-positive bacteria, cyanobacteria, and the alpha, beta, gamma, and delta subdivisions of proteobacteria, consistent with major bacterial groups found in trees derived from analyses with other molecules. However, some groupings (e.g., the chlamydiae, mycoplasmas, and green sulfur bacteria) are found in different positions than for trees obtained by using other molecular markers. A direct comparison to the most extensively used molecule in systematic studies, small-subunit rRNA, was made by deriving trees from essentially the same species set and using similar phylogenetic methods. Differences and similarities based on the two markers are discussed. Additionally, 31 group 2 sigma factors were analyzed in combination with the group 1 proteins in order to detect functional groupings of these alternative sigma factors. The data suggest that promoters recognized by the major vegetative sigma factors of eubacteria will contain sequence motifs and spacing very similar to those for the sigma70 sigma factors of Escherichia coli.
RECONSIDERING SOME ASPECTS OF THE TWO-TRIALS PARADIGM

A common standard for the demonstration of efficacy in a clinical submission is a statistically significant outcome in at least two pivotal trials (“two-trials convention”). When the data structures in different trials are sufficiently similar to allow pooling of the data across trials for a combined analysis, we argue here that such an analysis is a more logical and efficient basis for a judgment regarding efficacy. Criteria for combined analyses may be established, which ensure the same false positive rate protection as the two-trials convention. A combined analysis will generally have much more power than the corresponding application of the two-trials approach that has the same false positive rate protection. In addition, we describe the behavior of modified versions of pure combined analysis, which incorporate a formal standard for reproducibility of trial results by limiting the larger of the individual trial p-values. These modifications are shown to maintain the desirable behavior of the pure combined analysis, namely, higher power compared to the two-trials convention.
Production of an Amylase-Sensitive Bacteriocin by an Atypical Leuconostoc paramesenteroides Strain

An atypical Leuconostoc paramesenteroides strain isolated from retail lamb produced a bacteriocin, leuconocin S, that was inactivated by α-amylase, trypsin, α-chymotrypsin, protease, and proteinase K but not by lipase or heat treatment at 60°C for 30 min. Supernatants from culture broths produced two glycoprotein bands on sodium dodecyl sulfate-polyacrylamide gels; these had molecular weights of 2,000 and 10,000 and activity against Lactobacillus sake ATCC 15521. The crude bacteriocin preparation was bacteriostatic and dissipated proton motive force. Bacteriocin activity was produced over a wide pH range (5.2 to 7.9) on buffered agar medium, with an optimum pH of pH 6.15. The optimum pH for production in broth was 6.5 to 7.0.
Psychosocial Factors Affecting Adherence to Medical Regimens in a Group of Hemodialysis Patients

The present research was designed to identify psychosocial correlates of adherence among patients receiving hemodialysis at two outpatient clinics. The 116 participants were interviewed concerning their knowledge of treatment, health beliefs, treatment history, social support, personal characteristics, and adherence to the medication, diet and fluid-intake aspects of the regimen. In addition, medical record data were obtained on serum phosphorus and serum potassium levels, and on between-dialysis weight gains. The magnitude of relationships between predictors and adherence measures varied, depending on the method used to measure adherence. For the self-report measures, beliefs concerning the efficacy of the behavior and barriers to the behavior, along with reported family problems, proved to be consistent predictors. Other beliefs and characteristics did not contribute significantly. For the medical chart information, however, the predictive factors were less consistent. In general, situational factors seem to be the major contributors to patient adherence, and adherence itself is seen as a complex and multidimensional phenomenon.
SPECIFICITY OF RUSSIAN WORKERS' ATTITUDES AND BEHAVIOR IN TERMS OF THEIR PERSONAL FINANCES

В статье на материалах общероссийского исследования Института социологии РАН 2014 г. анализируются особенности установок и реального поведения в отношении своих финансовых средств российских рабочих. Проведено их сравнение с установками и поведе-нием населения страны в целом и лицами высококвалифицированного умственного труда в частности. Показано, что «запас прочности» рабочего класса очень мал как из-за от-носительно более низких доходов, так и потому, что свои свободные средства его пред-ставители, в отличие от лиц высококвалифицированного умственного труда, даже при равных доходах предпочитают тратить на текущее потребление, а не сберегать. Как это обстоятельство, так и низкий уровень доверия всех россиян и рабочих в особенности к любым организованным способам сбережений и инвестиций снижают внутренний инве-стиционный потенциал страны. Зафиксировано, что в России слабо применимы сегодня традиционные концепции, объясняющие финансовое поведение населения. Продемонстри-ровано, что высококвалифицированные рабочие, рабочие средней и низкой квалификации, а также рядовые работники торговли и сферы услуг очень близки между собой в отноше-нии их реального финансового поведения и доминирующих у них в этой сфере установок, и противостоят в этом отношении работникам, занимающим профессиональные позиции, традиционно относимые к среднему классу.
Non-pharmacological options may have role in postmenopausal osteoporosis

EDITOR—Cranney's editorial thoroughly covered the pharmacological options available for treating postmenopausal osteoporosis.1 Non-pharmacological treatments may also have a role, given the uncertainties in managing osteoporosis. Exercise is a simple intervention that can be advised for postmenopausal women with osteoporosis and is beneficial for other health …
Bdellovibrio and the intestinal flora of vertebrates

Bdellovibrio strain MS7 force-fed to fish and frogs via an intragastric tube did not become an integral component of the intestinal microflora. Strain MS7 fed to mice in drinking water was not recovered from the intestinal tract of mice. However, in vitro, the organism multiplied in intestinal contents of frogs and mice. Bdellovibrio inoculated into rabbit ileal loops was greatly reduced in number within 24 h. It was concluded that strains MS7 could be considered nonpathogenic to animals, at least when introduced into the digestive tract, and that it is not feasible at the present time to lyse pathogenic, gram-negative bacteria in the alimentary tract with Bdellovibrio.
Study of a new schizophrenomimetic drug; sernyl.

Introduction The production of model psychoses has been employed as a technique for testing hypotheses relevant to the causes, correlates, and treatment of schizophrenia since 1921, when De Jong demonstrated experimental catatonia by giving bulbocapnine to animals.5This method of study has received considerable impetus with the discovery of the hallucinogenic properties of lysergic acid diethylamide (LSD-25)22and other psychotomimetic agents. Objections have since been raised to the drawing of premature analogies between the psychotic-like states produced by drugs and the psychopathology of schizophrenia. Ebaugh has pointed out that "the difference between these pseudopsychoses and schizophrenia is so striking that such experimentation seems to have only tenuous relevance to the problem of schizophrenia. "7On the other hand, Wikler has asserted that "from the standpoint of the development of dynamic (causal) concepts about human behavior, it matters little whether or not model psychoses resemble schizophrenia. Drugs which modify
Optimal timing of invasive angiography in stable non-ST-elevation myocardial infarction: the Leipzig Immediate versus early and late PercutaneouS coronary Intervention triAl in NSTEMI (LIPSIA-NSTEMI Trial).

AIMS The optimal timing of intervention in non-ST-elevation myocardial infarction (NSTEMI) remains uncertain. The aim of this multicentre trial was to assess whether an immediate invasive approach is superior to an early invasive or a selective invasive approach with respect to reduction of large infarction. METHODS AND RESULTS Patients with NSTEMI were randomized to either an immediate (<2 h after randomization; n= 201), an early (10-48 h after randomization; n= 200), or a selective invasive approach with high invasive percentage (n= 201). The primary outcome was the peak creatine kinase (CK)-myocardial band (MB) activity during index hospitalization; key secondary clinical endpoints were the composite of (i) death and non-fatal infarction; (ii) death, non-fatal infarction, and refractory ischaemia; (iii) death, non-fatal infarction, refractory ischaemia, and rehospitalization for unstable angina within 6 months. The median time from randomization to angiography was 1.1 h in the immediate vs. 18.6 h in the early and 67.2 h in the selective invasive group (P< 0.001). There was no significant difference in the peak CK-MB activity between groups. The key secondary clinical endpoints were similar between groups at 6-month follow-up: death and infarction: 21.0 vs. 16.0 vs. 14.5%; P= 0.17; death, infarction, refractory ischaemia: 20.9 vs. 21.5 vs. 22.0%; P= 0.98; death, infarction, refractory ischaemia, rehospitalization: 26.0 vs. 26.5 vs. 24.5%; P= 0.91, respectively. CONCLUSIONS In NSTEMI patients, an immediate invasive approach does not offer an advantage over an early or a selective invasive approach with respect to large myocardial infarctions as defined by peak CK-MB levels, which is supported by similar clinical outcomes. ClinicalTrials.gov NCT00402675.
Gray matter abnormalities in autism spectrum disorder revealed by T2 relaxation

To the Editor: Branch vessel occlusion by snow plowing effect and jailing has been a concern in intracranial stenting. Jiang et al.1 reported a low rate of perforator stroke (3.0%) by coronary balloon-mounted stents. However, the figure could be an underestimate as CT rather than MRI was used for diagnosis. The editorial2 in the same issue described the current trend of using selfexpanding stent (Wingspan) in intracranial vasculature. The system allows submaximal balloon dilatation, which may reduce the risk of acute perforator occlusion. It is unclear whether the subsequent luminal gain of the parent artery through the sustained radial force of the self-expanding stent will lead to subacute jailing or occlusion of the perforators. In our center, paired MRI (preand post-stenting) was obtained to look for new infarcts in the perforator zone of the treated artery in patients who had angioplasty by Wingspan system. The post-stenting MRI was performed 4 months after the procedure. Among the six patients who had stenting at the perforator-rich arteries (M1 middle cerebral artery or basilar artery), none had clinical stroke or new infarcts evident on T2-weighted images in the immediate perforator zone.
A new PrfA‐regulated gene of Listeria monocytogenes encoding a small, secreted protein which belongs to the family of internalins

A mutant of Listeria monocytogenes EGD was constructed that carries an extended deletion removing the entire PrfA‐regulated gene cluster from plcA to plcB and a second deletion inactivating the inlA gene. Upon supplementation of this mutant with multiple gene copies of prfA, a protein of 30 kDa was detected in the supernatant of the mutant strain. The gene encoding this protein was obtained by direct and inverse polymerase chain reaction using oligonucleotide primers that were deduced from partial amino acid sequences of the purified 30 kDa protein. The amino acid sequence of the gene product revealed a protein of 297 amino acids that carried eight repeat units with high homology to those of the two known internalin proteins A and B. This secretory protein, termed internalin C, is much smaller than InlA or InlB and its complete sequence is related to the two known internalins. The gene inlC is transcribed into a monocistronic mRNA from a single promoter which shows a typical consensus sequence for PrfA‐binding at the position −40. In contrast to the transcription of the inlAB operon, which is downregulated after shift of an L. monocytogenes EGD culture from brain–heart infusion into minimum essential medium (MEM), transcription of inlC is induced in MEM like most of the other known PrfA‐regulated virulence genes. In addition, inlC is strongly transcribed in the cytoplasm of phagocytic J774 cells whereas inlA is poorly transcribed under these conditions, suggesting that internalin C may play a role in a late stage of L. monocytogenes infection rather than in the uptake of L. monocytogenes by non‐professional phagocytic cells. An inlC deletion mutant shows reduced virulence when tested in an intravenous mouse model, but intracellular replication of the mutant in Caco‐2 and J774 cells appears to be comparable with that of the wild‐type strain.