Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Propolis on moderate persistent asthma: A phase two randomized, double blind, controlled clinical trial

Objective: The aims of this study was to determine the effect of Propolis (resinous mixture that honey bees produce by mixing saliva and beeswax) on clinical and physiological findings of moderate persistent asthma. Materials and Methods: Fifty-two subjects aged 44.6±18.5 years old with moderate asthma and Forced expiratory volume in 1 second (FEV1) 60-79% of predicted, were enrolled in this clinical trial. We randomly allocated subjects to receive either propolis (75 mg three times a day) or a matched placebo for one month. Primary outcome was Asthma control test (ACT) score and secondary outcomes included dyspnea, spirometry, fractional exhaled nitric oxide (FENO) and sputum cytology including inflammatory cell. Sputum induction was done by hypertonic saline and cytology slides were stained by Papanicolaou stain. Results: Clinical findings significantly improved after the treatment. ACT scores significantly increased by using propolis (12.8±5.5 before and 18.1±4.99 after the trial), which was significantly higher than the placebo group (14.4±6.6 after the trial). The most significant physiological improvements were significant increases in FEV1, FV1/Forced vital capacity and expiratory flows. FENO showed significant decreases in the propolis group but increases in the placebo group. Cytological examination of sputum showed that the pattern of inflammation was eosinophilic in 44% subjects with an average eosinophil of 7.2±1.01%. Eosinophilia significantly decreased (p<0.05) by using propolis (7.2±1.01 and 4.3±3.1%, before and after treatment, respectively), but it significantly increased (p<0.04) in the placebo group (5.5±2.8, and 11.1±6.6%, before and after treatment, respectively). Conclusion: Propolis improved the clinical and physiological findings of moderate persistent asthma, and it was able to suppress eosinophilic inflammation.
Study on the determination and hypoglycemic activity in vitro of Taxus chinensis var. mairei polysaccharide

The polysaccharide of Taxus chinensis var. mairei was extracted by hot water,the content of polysaccharide was determined by phenol-sulfuric acid method;the HepG2 cells and alpha-glucosidase were used as receptor model to study the hypoglycemic activity in vitro of Taxus chinensis var. mairei polysaccharide. The results showed that the average content of polysaccharide from the branches and leaves of Taxus chinensis var mairei was 1.75%. When the linear range of anhydrous glucose was 1.507 to 13. 563μg/mL,there was a good linear relationship. The average rate of recovery was 97.54%,RSD was 1.60%. Using Metformin and Acarbose as the positive drug,crude polysaccharides from Taxus chinensis var. mairei could improve the glucose consumption of insulin-resistance HepG2 cells,it(IC50 38. 61mg/L)showed higher inhibitory activity to alpha-glucosidase than Acarbose(IC50 1081.41 mg/L). And it showed dose dependent for the inhibitory activity.
Thylakoid‐associated Polyamines Adjust the UV‐B Sensitivity of the Photosynthetic Apparatus by Means of Light‐harvesting Complex II Changes ¶

The sensitivity of the photosynthetic apparatus to ultraviolet‐B (UV‐B) irradiation was studied in cultures of unicellular green alga Scenedesmus obliquus incubated in low light (low photosynthetically active radiation intensity [LL]) and high light (high photosynthetically active radiation intensity [HL]) conditions, treated or not with exogenous polyamines. Biochemical and physicochemical measurement showed that UV‐B radiation induces a decrease in the thylakoid‐associated putrescine (Put) and an increase in spermine (Spm), so that the reduction of Put/Spm ratio leads to the increase of light‐harvesting complex II (LHCII) size per active reaction center and, consequently, the amplification of UV‐B effects of the photosynthetic apparatus. The separation of oligomeric and monomeric froms of LHCII from isolated thylakoids showed that UV‐B induces an increase in the oligomeric forms of LHCII, which was more intense in LL than in HL. By manipulating the LHCII size with exogenous polyamines, the sensitivity degree of the photosynthetic apparatus to UV‐B changed significantly. Specifically, the addition of Put decreased highly the sensitivity of LL culture to UV‐B because of the inhibitory effect of Put on the LHCII size increasing, wheres the addition of Spm enhanced the UV‐B injury induced in HL culture because of the increasing of LHCII size. The ability of the photosynthetic apparatus to recover the UV‐B induced changes was also investigated.
Corrigendum

In (1), the following error was published on page 254, left column, third paragraph. For prescribing the dose of CAPD in patients with known residual renal function, the same Dv ⁄V value, 0.304 (l ⁄24 hours) ⁄ l, should be entered in Eq. (3), but the target Kpt ⁄V ⁄Urea should be 1.70 – renal weekly Kt ⁄VUrea. The text was incorrect and should have read: For prescribing the dose of CAPD in patients with known residual renal function, the same D ⁄PUrea value, 0.8, should be entered in Eq. (3), but the targetKpt ⁄VUrea should be 1.70 – renal weekly Kt ⁄VUrea. From Eq. (3), the target Dv ⁄V decreases by 0.018 (l ⁄24 hours) ⁄ l for 0.1 increase in renal weeklyKt ⁄VUrea. We apologize for this error.
Type IV secretion system of Brucella

Brucellosis,which is caused by Brucella,is one of the most important bacterial zoonoses.It brings serious harm to stock raising,human health and animal-derived food security.Type IV secretion system of Brucella is essential for survival and multiplication within the host cells,which also play an important role in pathogenicity of Brucella.Type IV secretion system that is encoded by the virB operon is described as a family of multi-protein and crossing-envelope complex,and regulated by the same promoter.Type IV secretion system is also affected by multi-factor,which may be PH,temperature,nutrition and the type of the cells infected and it plays an important role in regulation intracellular and extracellular survival of Brucella.
Molecular cloning,characterization and expression analysis of LC3B from purse red common carp(Cyprinus carpio) exposed to cadmium

LC3(microtubule-associated protein 1light chain 3),located in autophagosome membranes after processing,serves as a major autophagy marker.In this study,we isolated its LC3Bisform from common carp(cyprinus carpio),one of the most important industrial aquatic animals in China using rapid amplification of cDNA ends(RACE). The cDNA of LC3Bgene is 2138-nt in length,containing a 378-nt open reading frame.The putative LC3Bencodes a peptide of 125amino acids,with a calculated molecular weight of 14.73kDa and an isoelectric point of 8.76. Multiple sequence alignment showed that the deduced amino acid sequence of common carp LC3Bhad the highest sequence identity(95.2%)and similarity(96.8%)to that of zebrafish.In a phylogenetic tree based on amino acid sequences from fish,mammalian,avian and amphibian LC3genes,the LC3proteins from different species were divided into three subgroups(LC3A, LC3Band LC3C). The common carp LC3Bformed a phylogenetic cluster with zebrafish,then other vertebrates.The mRNA transcript level of LC3Bwas detected in muscle,brain,gills,spleen,liver,blood,head kidney,kidney and heart tissues by RT-PCR analysis.LC3B mRNA transcript level in kidney was found significantly elevated after exposure to cadmium.This was markedly affected by exposure concentration, not by exposure time.
Decreased Levels of (6–4) Photoproduct Excision Repair in Hybrid Fish of the Genus Xiphophorus ¶

Selected hybridization in the fish genus Xiphophorus has been used for many years to study the genetics of malignant melanoma. Because DNA damage caused by ultraviolet radiation is implicated in the etiology of sunlight‐induced melanoma, the heritability of mechanisms that mitigate DNA damage is a matter of some interest. We examined nucleotide excision repair of the two major types of DNA‐damage induced by sunlight; the cyclobutane pyrimidine dimer (CPD) and the pyrimidine (6–4) pyrimidone dimer [(6–4)PD]. In most cases, removal of the (6–4) PD was more rapid than the CPD, and in many cases, the F1 hybrid showed reduced repair efficiency compared with the parental species. These data demonstrate reduced function in multienzyme hybrid systems and provide molecular support for potential reduced fitness in hybrid fish under conditions of environmental stress.
Direct Visualization of Abasic Sites on a Single DNA Molecule Using Fluorescence Microscopy ¶

A new method was developed to allow direct visualization of damaged sites on individual DNA molecules. Fluorescence in situ hybridization on extended DNA molecules was modified to detect a single abasic site. Abasic sites were specifically labeled with a biotinylated aldehyde‐reactive probe and fluorochrome‐conjugated streptavidin. The light emitted by a single fluorochrome–DNA complex was calibrated. The number of abasic sites on the DNA molecule was estimated by counting each fluorochrome–DNA complex. The present study directly visualized and characterized the abasic sites of single DNA molecules.
Light Absorption by the Chlorophyll a–b Complexes of Photosystem II in a Leaf with Special Reference to LHCII ¶

To investigate the light‐harvesting properties of the Photsystem II chlorophyll (chl) a‐b complexes (major light‐harvesting complex of Photosystem II [LHCII], CP24, CP26, CP29) in a mature leaf under natural “daylight” illumination, the absorption spectra of the isolated complexes were converted into the photon absorption spectrum (1‐T) within a leaf, using the approach of Rivadossi et al. ([1991] Photosynth. Res. 60, 209–215). In the Qy region, significant enhancement of light harvesting by the chl b electronic transitions, with respect to the absorption spectra (optical density [od]), as well as a large and generalized increase (between two‐ and four‐fold) associated with the vibrational bands of both chl a and b, was observed, which acquires an important light‐harvesting role (approximately 30–40% of total). In the Soret region, a small increase in light harvesting by chlb was indicated. To gain more detailed information on these aspects the light harvesting of LHCII in a leaf was investigated. This required describing the pigment absorption (chla and b, carotenoids) in the LHCII OD spectrum in therms of spectral subbands, which were subsequently used to estimate the relative light harvesting of each pigment type in LHCLL in leaf. When the entire visible spectral interval between 400 and 730nm is considered, the chl a light harvesting is essentially unchanged with respect to the absorption spectrum (OD) of isolated LHCII, whereas the chl b contribution is 20% higher and the carotenoids are 33% lower. The relative enhancement of chl b absorption is principally associated wiht the Qy electronic transition region, the light‐harvesting contribution of which becomes prominent in the leaf.
Arg‐72 of pharaonis Phoborhodopsin (Sensory Rhodopsin II) is Important for the Maintenance of the Protein Structure in the Solubilized State ¶

In bacteriorhodopsin (bR), Arg‐82bR has been proven to be a very important residue for functional role of this light‐driven proton pump. The arginine residue at this position is a super‐conserved residue among archaeal rhodopsins. pharaonis phoborhodopsin (ppR; or called as “pharaonis sensory rhodopsin II”) has its absorption maximum at 498 nm and acts as a sensor in the membrane of Natronobacterium pharaonis, mediating the negative phototaxis from the light of wavelength shorter than 520 nm. To investigate the role of the arginine residue (Arg‐72ppR) of ppR corresponding to Arg‐82bR, mutants whose Arg‐72ppR was replaced by alanine (R72A), lysine (R72K), glutamine (R72Q) and serine (R72S) were prepared. These mutants were unstable in low concentrations of NaCl and lost their color gradually when the proteins were solubilized with 0.1%n‐dodecyl‐β‐D‐maltoside. The order of instability was R72S > R72A > R72K > R72Q > the wild type. The rates of denaturation were reduced in a solution of high concentrations of monovalent anions.
Reduction in DNA Synthesis During Two‐photon Microscopy of Intrinsic Reduced Nicotinamide Adenine Dinucleotide Fluorescence ¶

Two‐photon laser scanning microscopy (TPLSM) of endogenous reduced nicotinamide adenine dinucleotide (NAD(P)H) provides important information regarding the cellular metabolic state. When imaging the punctate mitochondrial fluorescence originating from NAD(P)H in a rat basophilic leukemia (RBL) cell at low laser powers, no morphological changes are evident, and photobleaching is not observed when many images are taken. At higher powers, mitochondrial NAD(P)H fluorescence bleaches rapidly. To assess the limitations of this technique and to quantify the extent of photodamage, we have measured the effect of TPLSM on DNA synthesis. Although previous reports have indicated a threshold power for “safe” two‐photon imaging, we find the laser power to be an insufficient indicator of photodamage. A more meaningful metric is a two‐photon‐absorbed dose that is proportional to the number of absorbed photon paris. A temporary reduction of DNA synthesis in RBL cells occurs whenever a threshold dose of approximately 2 × 1053 photon2 cm−4 s−1 is exceeded. This threshold is independent of laser intensity when imaging with average powers ranging from 5 to 17 mW at 740 nm. Beyond this threshold, the extent of the reduction is intensity dependent. DNA synthesis returns to control levels after a recovery period of several hours.
UV Index Experimental Values During the Years 2000 and 2001 from the Spanish Broadband UV‐B Radiometric Network ¶

An analysis is made of experimental ultraviolet erythemal solar radiation data measured during the years 2000 and 2001 by the Spanish UV‐B radiation evaluation and prediction network. This network consists of 16 Robertson–Berger type pyranometers for evaluating solar erythemal radiation and five Brewer spectroradiometers for evaluating the stratospheric ozone. On the basis of these data the Ultraviolet Index (UVI) was evaluated for the measuring stations that are located either in coastal regions or in the more densely populated regions inland on the Iberian Peninsula. It has been checked that in most cases the maximum irradiance values corresponded to solar noon, although there were exceptions that could be explained by cloudiness. The maximum experimental values of the UVI were around 9 during the summer, though frequently passing this value at the inland measurement stations. The annual accumulated dose of irradiation on a horizontal plane has also been studied, as well as the evolution through the year in units of energy, standard erythemal doses and minimum erythemal doses, according to different phototypes.
Retrospective Study in Primary Central Nervous System Lymphoma: The Impact of Treatment Strategies of the Last 20 Years

The most important risk factor for primary central nervous system lymphoma (PCNSL) development is immunodeficiency [1-7]. PCNSL was traditionally treated with cranial irradiation, i.e., whole-brain radiotherapy (WBRT). WBRT may become complicated by the development of chronic and late neurotoxicity. Treatment with chemotherapy alone was suggested to avoid such complications. The ideal treatment of PCNSL is poorly demarcated. The use of wide variety of methotrexate (MTX) based treatment regimens resulted in excellent survival rates. Nevertheless, disease control with these regimens is unpredictable.
Study on the inhibitory action on CVB3 replication in vitro by the extracts from Selaginella moelledorfii Hieron

OBJECTIVE To investigate the anti-Coxsackie virus B3(CVB3) activity of different extracts from Selaginella moelledorfii Hieron,and their antiviral mechanism.METHODS Hep-2 cells infected by CVB3 were cultured with different extracts for 72 hours and their inhibitory effect on CVB3 replication were evaluated by cell survival rates according to MTT assay. RESULTS In the Hep-2 cell system,some of the extracts could inhibit cytopathic effect(CPE) of cells infected by CVB3,and increase cells,survival rates. The active sites were lipid soluble biflavone and hydro-soluble flavonoid glycoside. CONCLUSION Selaginella moelledorfii Hieron can be taken into account as new potential resource plants with anti-CVB3 action.
Can the quantity of cell‐free fetal DNA predict preeclampsia: a systematic review

In Martin et al., the first name and family name of the third author were incorrectly identified. The correct name is: <first name>Martina <family name>Badell The correct author listing is: Angela Martin, Iris Krishna, Martina Badell and Amber Samuel We apologize for this error. Reference Martin A, Krishna I, Martina B, Samuel A. Can the quantity of cell-free fetal DNA predict preeclampsia: a systematic review. Prenat Diagn 2014;34:685–91.
Reference Scenarios and Key Performance Indicators for 5G Ultra-dense Networks

The so-called 5G will revolutionize the way we live, and work. In order to demonstrate the profound changes, we can expect to experience within the next 5 to 10 years, we present use cases for the planned research within the TeamUp5G project. Some use cases are strongly linked to the network layer and aim at developing solutions capable of optimizing the main promising benefits of 5G: extremely low latency and extremely high bandwidth (e.g., handle video streams, traffic congestion, user profiles), in the most efficient way possible. Other use cases focus on commercial applications that make use of middleware applications to enhance their performance. The latter fall into two main areas: real-time virtual reality and live video streaming, which are extremely demanding in terms of latency and bandwidth to provide an acceptable QoE/QoS to multiple users. The use cases presented are built assuming that 5G is essential for their support with appropriate QoE/QoS. Key performance indicators and their range of variation are also identified.
A Paern-based Approach for an Early Detection of Popular Twier Accounts

Social networks (SN) are omnipresent in our lives today. Not all users have the same behaviour on these networks. If some have a low activity, rarely posting messages and following few users, some others at the other extreme have a signi � cant activity, with many followers and regularly posts. The important role of these popular SN users makes them the target of many applications for example for content monitoring or advertising. It is therefore relevant to be able to predict as soon as possible which SN users will become popular. Inthis work, we propose a technique for early detection of such users based on the identi � cation of characteristic patterns. We present an index, � 2 " , which allows a scaling up of our approach to large social networks. We also describe our � rst experiments that con � rm the validity of our approach.
Numerical and Experimental Investigations on a Dimpled Savonius Vertical Axis Wind Turbine

This research deals with the design and fabrication of novel designs as a mean to harness wind energy using a ‘Savonius’ Turbine. It is generally employed to harvest the low to very low wind speed potentials. They can particularly be of high use in the regions where the wind speeds are not high enough for installing conventional horizontal axis wind turbines. The paper introduces a novel concept of testing a Savonius wind turbine with dimple structures on its blades and investigating its effect on the turbine’s efficiency. The study also deals with the experimental validation of the effect of using a converging ducted structure with a single stage and double stage configurations of Savonius wind turbine, apart from comparing the turbine’s performance with and without endplates. The results of the study proves that power coefficient increases with the addition of dimple structures on the blades and it can be further augmented by using a ducted structure with the Savonius turbine.
Non-local linear-response functions for thermal transport computed with equilibrium molecular-dynamics simulation

We establish an approach to compute linear-response functions to elucidate heat waves and non-local thermal transport. The theory is able to describe the response of a system to external heat sources that are nonuniform in space and time. The response functions are computed using equilibrium molecular-dynamics simulations of an Ar crystal modeled using the standard Lennard-Jones potential. It is shown that for low temperatures and short length scales, transport can be partially or even completely ballistic, with the response primarily limited by the group velocity of lattice waves. By contrast, at longer length scales and higher temperatures, the response functions correspond more closely to diffusive transport characteristic of Fourier's law. It is also shown how the effective thermal conductivity can be determined in a partially-ballistic regime. The results demonstrate the known reduction in the effective thermal conductivity observed when system dimensions are smaller than the mean-free path for lattice waves. Finally, we show how determination of the relevant response functions can be used to model heating of a crystal without requiring additional atomic-scale simulations. Differences between computed results and predictions from Fourier's law represent wave-like, partially-ballistic transport.
Referral Bias in Coronary Micro vascular Dysfunction: a Retrospective Study

Obstructive coronary artery disease (CAD) is a well-defned condition and a signifcant cause of myocardial ischemia. Patients with angina typically undergo non-invasive cardiac investigations initially as a means of risk-stratifying. Coronary angiography is the diagnostic test of choice for patients with chest pain (angina) and signs of myocardial ischemia on noninvasive testing. Typically CAD is diagnosed based on the patency of the epicardial coronary arteries [1]. Approximately 30% of patients with angina have non-obstructive arteries on coronary angiogram and area diagnostic and management challenge for clinicians, contributing to signifcant social and economic burden [2]. They undergo multiple angiograms, experience a hospitalization rate 80% greater than patients with single vessel CAD and up to 50% have persistent symptoms leading to functional disability [3]. Historically, this population was given reassurance as long-term prognosis was believed to be benign [4]. We now know that a subset of these patients sui¬ÂÂer from coronary micro vascular dysfunction (MVD) [1] and are at increased risk for adverse cardiac events including congestive heart failure (CHF), myocardial infarction (MI), left ventricular (LV) dysfunction, and death [1,5]. The Cardiovascular Integrated Physiology (CVIP) program at Southlake Regional Health Centre (SRHC) investigates and manages patients with MVD. CVIP has received referrals for only a small fraction of patients with angina and non-obstructive angiograms at SRHC. It is unclear what factors promote referral. We hypothesized that referrals were not random and that the clinical characteristics of the referred patients dii¬ÂÂered from the larger population of patients with angina and nonobstructive CAD. The primary objective of this study was to determine whether the patients referred to CVIP are an accurate representation of the overall population of patients with angina and non-obstructive coronary arteries.