Miracle-dz/UniFAR-FTUs huggingface dataset:
AUTOINDUCTION AND ALDEHYDE CHAIN‐LENGTH EFFECTS ON THE BIOLUMINESCENT EMISSION FROM THE YELLOW PROTEIN ASSOCIATED WITH LUCIFERASE IN Vibrio fischeri STRAIN Y‐1b

Abstract— A new strain of the yellow‐light emitting bacterium Vibrio fischeri Y‐lb, possessing a higher fraction of its emission in the yellow, was isolated and characterized. The in vivo yellow to blue ratio (Y/B) increases during growth in parallel with the autoinduction of luciferase, reaching a value above 5 at 17°C. This Y/B increase is attributed to concomitant increases in the intracellular levels of both luciferase and the specific protein associated with yellow emission, the yellow fluorescent protein (YFP), and to the association of the two proteins at higher concentrations. The yellow emission is rapidly but reversibly lost by brief exposures to a temperature of 27°C (5 s), and irreversibly so at 40°C, attributed to dissociation of the two proteins. A concentrated crude extract emits almost exclusively yellow light in the in vitro reaction, and theY/B ratio decreases with dilution. The Y/B ratio in vitro is also dependent on the aldehyde chain length; experiments with aldehyde analogs suggest that the aldehyde binding site of luciferase must be occupied for YFP to stimulate light emission.
Experimental obliteration of the preprophase band alters the site of cell division, cell plate orientation and phragmoplast expansion in Adiantum protonemata

the premitotic nuclear positioning is delayed markedly and irregularly oriented cell plates are frequently observed. Re-irradiation with red light also causes an increase in cells without preprophase bands (PPBs) at prophase and the irregular expansion of the phragmoplast at late telophase, while early phragmoplast microtubule (MT) organization takes place normally. These data suggest the indirect involvement of PPBs in the guidance of phragmoplast expansion.
A web service‐based internet of things framework for mobile resource augmentation

Mobile devices are the primary communication tool in day to day life of the people. Nowadays, the enhancement of the mobile applications namely IoTApps and their exploitation in various domains like healthcare monitoring, home automation, smart farming, smart grid, and smart city are crucial. Though mobile devices are providing seamless user experience anywhere, anytime, and anyplace, their restricted resources such as limited battery capacity, constrained processor speed, inadequate storage, and memory are hindering the development of resource‐intensive mobile applications and internet of things (IoT)‐based mobile applications. To solve this resource constraint problem, a web service‐based IoT framework is proposed by exploiting fuzzy logic methodologies. This framework augments the resources of mobile devices by offloading the resource‐intensive subtasks from mobile devices to the service providing entities like Arduino, Raspberry PI controller, edge cloud, and distant cloud. Based on the recommended framework, an online Repository of Instructional Talk (RIoTalk) is successfully implemented to store and analyze the classroom lectures given by faculty in our study site. Simulation results show that there is a significant reduction in energy consumption, execution time, bandwidth utilization, and latency. The proposed research work significantly increases the resources of mobile devices by offloading the resource‐intensive subtasks from the mobile device to the service provider computing entities thereby providing Quality of Service (QoS) and Quality of Experience (QoE) to mobile users.
Microsatellite or Simple Sequence Repeat (SSR) instability depends on repeat characteristics during replication and repair

Microsatellites or simple sequence repeats (SSRs) are ubiquitous in genomes studied so far. SSR mutations or microsatellite instability (MSI) can result in either expansion or contraction of repeat units. These alterations can lead to disorders like cancer and neurodegeneration. As gleaned from studies, it appears that SSR mutations are dependent on motif size, nucleotide content and SSR length. Besides these, position of SSRs in genome (coding or non-coding sequences), presence on leading or lagging strand and distance from origin of replication also affect SSR mutations. Further, fidelity of replication and repair machinery as well as epigenetic factors may influence repeat stability. This review summarizes some of these aspects of SSR mutations that occur especially during DNA replication/repair besides epigenetic factors that influence SSR contraction or expansion.
Appropriate mathematical models for describing the complete lactation of dairy sheep

Despite milk being an important product from sheep, there are very few reports of milk production from the complete lactation of dairy sheep. The Improved Awassi in Israel is kept under an intensive system of management with lambs being weaned soon after birth. Records from one such flock were analysed to investigate the suitability of various mathematical functions for describing milk yield from the complete lactations of dairy sheep. This included a consideration of whether the functions could cope with short lactations, a characteristic of dairy sheep, and a limited number of test-day records per lactation. Four non-linear mathematical functions were investigated (Wood, Morant, Grossman and Pollott), two of which could also be fitted in a linear and a linear weighted form (Wood and Morant). These functions were fitted to weekly data from a ‘typical Awassi lactation curve’, represented by least squares means of daily milk yield from each week of a 40-week lactation derived from an analysis of 25605 test day records. Characteristics of the lactation were calculated from the functions, such as total milk yield, day and level of peak yield and persistency. These functions were also fitted to 1416 individual lactation records of up to 10 test-day records per lactation. The value of the functions was investigated using the residual mean square (RMS) of the fitted curve as an indicator of how well each function described the lactation. Forms of these functions with a reduced number of parameters were also investigated. The non-linear functions always fitted the data with a lower RMS than their linear equivalent and the weighted form of the linear functions always had a lower RMS than the unweighted form. Of the linear functions, Morant fitted better than Wood. Of the non-linear functions Grossman, Morant and Pollott (additive and multiplicative) fitted the data equally as well but better than Wood. The various functions predicted characteristics of the lactation curve differently; the Wood functions tended to overestimate yield in early lactation and the Morant functions underestimated peak yield. No function was better suited to short lactations than another. However the three-parameter function of Morant, Pollott multiplicative and Pollott additive were considered to be the most suitable for describing the complete lactation of dairy sheep.
VARIAÇÕES SAZONAIS E ONTOGÊNICAS NA DIETA NATURAL DE Callinectes danae SMITH, 1869 (DECAPODA, PORTUNIDAE) NA LAGOA DA CONCEIÇÃO, FLORIANÓPOLIS, SC.

SEASONAL AND ONTOGENETIC VARIATIONS ON THE FEEDING NATURAL OF Callinectes danae SMITH, 1869 (DECAPODA, PORTUNIDAE) FROM LAGOA DA CONCEICAO, FLORIANOPOLIS, SC, BRAZIL. The diet of Callinectes danae from Lagoa da Conceicao were examined for seasonal and ontogenetic variations. A total of 456 males and 527 females were caught. The diet of the species was not sex related. The trophic spectrum of C. danae is composed by 35 items were assembled in 14 categories. Seasonal and ontogenetic variations in the species was determined by the availability and capacity of capturing prey in the area.
α‐Amylase adsorption on starch crystallites

The goal of this work was to characterize the adsorption of Bacillus subtills α‐amylase onto crystalline starchy materials of the B‐type polymorph. Monodisperse spherulitic particles (R ż6; 5.0 μm), essentially resistant to α‐amylolysis at 25°C were prepared from short amylose chains (DPn ≈ 15). The α‐amylase adsorbed specifically onto the spherulites, and adsorption was found to be a prerequisite step for hydrolysis. Adsorption was inhibited by the presence of maltose and maltotriose in the reaction mixture. Adsorption isotherm of the enzyme on the particles showed a well developed plateau of 1.62 μg/cm2 at 25°C corresponding to a monolayer adsorption process. The binding free energy calculated from the initial slope of the isotherm was ΔG ≈ −20.7 kJ/mol. This is smaller than published values for the binding of α‐amylase to soluble amylosic chains (ΔG < −30 kJ/mol).
Sp1 and Sp3 Are Important Regulators of AP-2γ Gene Transcription1

Abstract AP-2γ is a member of the AP-2 transcription factor family, is highly enriched in the trophoblast cell lineage, and is essential for placental development. In an effort to identify factors regulating AP-2γ gene expression, we isolated and characterized the promoter and 5′-flanking region of the mouse and human AP-2γ genes. The transcription start site of the mouse AP-2γ gene was mapped by primer extension and 5′ rapid amplification of cDNA ends. Deletion analysis of the 5′-flanking region revealed a 704-base pair (bp) sequence located approximately 6 kilobases (kb) upstream of the transcription start site that is required for enhanced expression in trophoblast cells. Additional gene transfer studies showed that basal promoter activity resides within a highly conserved, approximately 200-bp DNA sequence located immediately upstream of the transcription start site. The conserved region is highly GC-rich and lacks typical TATA or CCAAT boxes. Multiple potential Sp- and AP-2-binding sites are clustered within this region. Electrophoretic mobility shift assays demonstrated that Sp1 and Sp3 bind to three sites in the promoter region of the mouse AP-2γ gene. Combined mutation of the three putative Sp sites reduced promoter activity by 80% in trophoblast and nontrophoblast cells, demonstrating the functional importance of these sites in regulating AP-2γ gene expression. In summary, we have identified a potential trophoblast cell-specific regulatory element located approximately 6 kb upstream of the murine AP-2γ gene transcription start site, and we have shown that Sp1 and Sp3 bind to cis-regulatory elements located in the promoter proximal region and contribute to basal promoter activity.
Endocrine disruptors: a gender affair

Introduction The increase in non-communicable diseases in humans and wildlife over the past 40 years indicates an important role of the environment in disease aetiology. In particular, a huge amount of literature demonstrates the role of environmental endocrine disrupting chemicals in the pathogenesis of several diseases. Nowadays, it is clear that fundamental principles of endocrinology must be applied to the design and execution of studies on endocrine disrupting chemicals to be cognizant that the specific actions of individual hormones often change with development and ageing, they may be different in males and females, and may be mediated by different receptor isoforms expressed in different tissues or at different life stages. These statements are particularly true when assessing the hazard of endocrine disrupting chemicals against oestrogen and androgen actions in that these hormones are crucial determinants of sex-related differences in anatomical, physiological, and behavioural traits which characterise male and female physiology. This review aims to dissect the role exerted by endocrine disrupting chemicals in modulating androgen and oestrogen receptor activities to define the possible sex-related responses to these compounds. Data discussed here lead to a paradigm shift that challenges the concept that female organs are sensitive only to oestrogens (and anti-oestrogens), and male organs are sensitive only to androgens (and anti-androgens) because the pathways of sex steroid hormone biosynthesis as well as androgen and oestrogen response elements are found in both sexes. Thus, an overall estrogenic effect would occur in males exposed to endocrine disrupting chemicals, which could change the male hormonal milieu assured by a characteristic high ratio of androgen signals with respect to oestrogen signals. The opposite could also be envisioned in females. Conclusion Data on endocrine disrupting chemical action mechanisms are still unclear, confused and sometimes contrasting. Molecular studies in vitro and with in vivo animal models of both sexes are needed to identify pathways for endocrine disrupting chemical influence on sex steroid target tissues. In addition, studies on endocrine disrupting chemicals on several of these systems are much underrepresented, and these fields need to be expanded. These experimental approaches can surely highlight endocrine disrupting chemical disrupting action mechanism(s) in the future.
Do Linguistic Features Help Deep Learning? The Case of Aggressiveness in Mexican Tweets

In the last years, the control of online user generated content is becoming a priority, because of the increase of online aggressiveness and hate speech legal cases. Considering the complexity and the importance of this issue, this paper presents an approach that combines the deep learning framework with linguistic features for the recognition of aggressiveness in Mexican tweets. This approach has been evaluated relying ona collection of tweets released by the organizers of the shared task about aggressiveness detection in the context of the Ibereval 2018 evaluation campaign. The use of a benchmark corpus allows to compare the results with those obtained by Ibereval 2018 participant systems. However, looking at the achieved results, linguistic features seem not to help the deep learning classification for this task.
TGF-β increases cholesterol efflux and ABC-1 expression in macrophage-derived foam cells: opposing the effects of IFN-γ

The regulation of ATP-binding cassette transporter 1 (ABC-1) expression by cytokines present within the micro- environment of the atheroma may play an important role in de- termining the impact of reverse cholesterol transport on the atherosclerotic lesion. We recently reported that the macro- phage-activating cytokine interferon (IFN)- � inhibited both cholesterol efflux and ABC-1 expression. In the present study, we investigated the effects of transforming growth factor (TGF)- � , a cytokine also apparent within the atheroma, on cho- lesterol efflux, ABC-1 expression, and its ability to antagonize the inhibitory effects of IFN- � . TGF- � significantly increased cholesterol efflux in macrophage-derived foam cells from apo- lipoprotein E (apoE) knockout mice, with maximal effects appar- ent at 300 pg/ml. The increases in efflux occurred without any effect on the passive diffusion component of efflux mediated by � -cyclodextrin. Furthermore, the increase in cholesterol ef- flux occurred without any changes in free or esterified choles- terol pools and was consistent with an increase in both ABC-1 message and protein. Finally, TGF- � was also demonstrated to inhibit the IFN- � -mediated down-regulation of ABC-1. These results further demonstrate the importance of cytokine cross- talk to impact the process of reverse cholesterol transport through a multitude of processes including the regulation of ABC-1. — Panousis, C. G. , G. Evans, and S. H. Zuckerman. TGF- � increases cholesterol efflux and ABC-1 expression in macrophage-derived foam cells: opposing the effects of IFN- � . J. Lipid Res. 2001. 42: 856-863.
COMPARAÇÃO DA SÍNTESE DE CELULASES PELOS FUNGOS Trichoderma reesei E O FSDE15

RESUMO – Celulases são enzimas que constituem um complexo capaz de atuar sobre materiais celulósicos, promovendo sua hidrólise. A produção eficaz de celulases é importante para a definição de biorrefinaria, na utilização de materiais lignocelulósicos renováveis para produção de combustível e produtos químicos com alto valor agregado. Este trabalho avaliou a síntese de celulases por dois fungos, o Trichoderma reesei, conhecido na literatura como bom produtor de celulases, cedido pela Fundação André Tosello, e o FSDE15, isolado do solo de uma usina sucroalcooleira. Os cultivos foram realizados utilizando farelo de trigo como substrato, 90 gramas com umidade de 50%, dispostos em frascos erlenmeyer de 1000 mL e esterilizados. Após o resfriamento, o meio foi inoculado com uma concentração de 10 6 esporos/g e incubados sob condições estáticas em estufa a 37°C, durante 6 dias com análises de atividade de CMCase realizadas a cada 24 horas de cultivo. O pico de atividade de CMCase para os fungos avaliados foram com 4 dias de cultivo.
Investigation of genetic divergence in local sunflower hybrids and inbred lines by applying morphological markers

Knowledge of genetic diversity is the basic requisite for any plant breeding program. Seventeen sunflower hybrids and fifteen inbred lines including ten Cytoplasmic male sterile lines and five restorer lines were evaluated at the experimental area of National Agriculture Research centre, Islamabad, Pakistan during autumn-2011. The objective of study was to investigate the genetic diversity, categorize the proficient cross combiners and approximate characters association among sunflower hybrids and inbred lines. Data was estimated on i.e. flower initiation days, full flowering days, full developmental days, height of plant, disk diameter, stem thickness, leaves per plant, hundred achenes weight, achenes yield and oil content percentage. The maximum achenes yield was contributed by Hysun-33 2119 kg ha-1followed by SMH-0924 and SMH-0925, SMH-1028 and SMH-0926 were suggested as potential significant hybrids for future breeding plans to incorporate maximum achenes yield and oil content percentage. The CMS-11, CMS-25 and CMS-10 were long statured with vigorous stem and all the restorers were early maturing recommended for including in hybridization program to generate high heterotic factions. Cluster diagram based on euclidean dissimilarity matrix grouped hybrids as well as inbred lines in four groups. The group I and IV consist of four whereas group II and group III have three and five hybrids respectively. All the restores were clustered in III and IV and CMS in I and II groups respectively. According to principle component analysis the first two components contribute 64% and 89% in total variation in hybrids as well as in inbred lines respectively.
Effects of Tasco in Alleviation of Heat Stress in Beef Cattle

A study was conducted to evaluate the influence of Ascophyllum nodosum (Tasco) on rectal temperature, respiration rates, and in situ NDF disappearance in cattle exposed to elevated ambient temperature. Twenty-four Angus crossbred steers (average BW = 300.6 kg) were randomly assigned to treatments using a 2 × 2 factorial arrangement: 1% Tasco vs. no Tasco (control), and thermoneutral (TN) vs. heat load (HL) conditions. Three steers in each room were assigned to the Tasco vs. control treatment. In period 1 (10 d), steers were acclimated to chambers at TN (19°C) conditions. Subsequently, for periods 2 and 3, HL was maintained at a daytime high of 36°C and a nighttime low of 19 and 31°C, respectively. For period 4, temperature conditions (TN and HL treatments) were reversed and period 2 HL conditions were imposed. In periods 2 (P < 0.09) and 4 (P < 0.05), Tasco lowered DMI. In period 2, the control steers had greater (P < 0.0001) differences between maximum and minimum rectal temperatures than steers in the Tasco treatment; this difference occurred between d 4 and 8 for the HL group. In periods 2 and 3, the maximum and minimum differences in respiration rate values were greater (P < 0.05) under HL conditions for the Tasco vs. control treatment. In period 4, the maximum respiration rate response to HL tended to be greater (P = 0.07) under HL conditions for the control vs. Tasco treatment. In period 2, a trend existed for steers in the Tasco treatment to have a greater (P < 0.1) rate of in situ NDF disappearance than control steers. In conclusion, Tasco appeared to reduce rectal temperature for 3 to 4 d.
Superhydrophilic surfaces from short and medium chain solvo-surfactants

Pure monoglycerides (GM-Cs) and glycerol carbonate esters (GCE-Cs) are two families of oleochemical molecules composed of a polar part, glycerol for GM-Cs, glycerol carbonate for GCE-Cs, and a fatty acid lipophilic part.From a chemical point of view, GM-Cs include two free oxygen atoms in the hydroxyl functions and one ester function between the fatty acid and the glycerol parts. GCE-Cs contain two blocked oxygen atoms in the cyclic carbonate backbone and three esters functions: two endocyclic in the five-membered cyclic carbonate function, one exocyclic between the fatty acid and glycerol carbonate parts. At the physico-chemical level, GMCs and GCE-Cs are multifunctional molecules with amphiphilic structures: a common hydrophobic chain to the both families and a polar head, glycerol for GMs and glycerol carbonate for GCE-Cs. Physicochemical properties depend on chain lengths, odd or even carbon numbers on the chain, and glyceryl or cyclocarbonic polar heads.The solvo-surfactant character of GM-Cs and overall GCE-Cs were discussed through the measurements of critical micellar concentration (CMC) or critical aggregation concentration (CAC). These surface active glycerol esters/glycerol carbonate esters were classified following their hydrophilic/hydrophobic character correlated to their chain length (LogPoctanol/water = f(atom carbon number)). Differential scanning calorimetry and optical polarized light microscopy allow us to highlight the selfassembling properties of the glycerol carbonate esters alone and in presence of water. We studied by thermal analysis the polymorphic behaviour of GCE-Cs, and the correlation between their melting points versus the chain lengths.Coupling the self-aggregation and crystallization properties, superhydrophilic surfaces were obtained by formulating GM-Cs and GCE-Cs. An efficient durable water-repellent coating of various metallic and polymeric surfaces was allowed. Such surfaces coated by self-assembled fatty acid esters in a stable coagel state present a novel solution for the water-repellent coating of surfaces.
Epidemiological Study on the Impact of Vaccination Programs on Antigenic Relatedness, Genetic Characterization of Highly pathogenic Avian Influenza H5N1

Two HPAI H5N1 viruses were isolated from vaccinated layer and broiler commercial poultry farms in Egypt at years 2011 and 2013; respectively. By phylogenetic analysis, the viruses fall into two genetically diverse clades: (i) A/chicken/Egypt/VRLCU67/2011 classified as a variant virus, clade 2.2. 1.1; and (ii) A/chicken/Egypt/13VIR3729-4/2013 classified as a classic virus, clade 2.2. 1. Cross HI-test confirmed that the reaction between the two viruses is weak; furthermore, it showed the antigenic diversity between viruses belong to different clades and antigenic groups. Antigenic relatedness was calculated between six AI antigens and their antisera representing the different clades and antigenic groups circulated in Egyptian field; including the A/chicken/Egypt/VRLCU67/2011 strain which showed very low R-values with the other viruses’ groups; ranging from 17 % to zero. Results demonstrated the genetic and antigenic diversity of the variant viruses and how can the vaccine seed be a weak point in the vaccination program that could be broken by the drifted viruses antigenically distant from the vaccine strain.