Miracle-dz/UniFAR-FTUs huggingface dataset:
Does Obstructive Sleep Apnea Increase Cognitive Deficits in Pediatric Sickle Cell Disease?

Abstract Objectives: Although pediatric obstructive sleep apnea (OSA) is estimated to affect 2–3% of the general population, its prevalence in sickle cell disease (SCD) is much higher, with research suggesting a prevalence rate of upwards of 40%. Despite the similar underlying pathophysiological mechanisms of neurocognitive effects in pediatric OSA and SCD, there is a scarcity of information on how these two conditions interact. The aim of this study was to better understand the contribution of sleep apnea to neurocognitive deficits in children diagnosed with SCD. Method: This study assessed cognitive function in 26 children with comorbid SCD and OSA, 39 matched comparisons with SCD only, and 59 matched comparisons in children without a chronic health condition. Results: There were significant differences on measures of processing speed and reading decoding, with children without a chronic health condition scoring better than both chronic health condition groups. Additionally, the no chronic health condition group performed better on a test of quantitative knowledge and reasoning and a test of visual–spatial construction than the SCD-only group. Contrary to our hypotheses, there were no between-group differences suggesting an additive impact of OSA on cognition. Exploratory analyses revealed associations within the group that had OSA showing that more severe OSA correlated with lower performance on measures of processing speed and quantitative knowledge/reasoning. Conclusions: Children with comorbid OSA and SCD do not present with greater deficits in cognitive functioning than children with SCD alone. However, severe OSA may confer additional risk for neurocognitive impairments.
Investigating Fine-Scale Residential Segregation By Means of Local Spatial Statistics

The paper presents a GIS-based approach to estimating individual household residential segregation based on three sources of information: a detailed geo-referenced dataset of family characteristics obtained from the 1995 Israeli Census of Population and Housing, subjective data on individuals' estimates of their house's neighborhood, and detailed GIS maps of urban infrastructure. The potential of the proposed approach is illustrated by studying Jewish-Arab residential segregation in the Yaffo area of Tel Aviv. The combination of detailed objective and subjective geo-referenced data provide the basis for intensive fine-scale urban studies and local planning interventions.
Determinants of Self-Care in Patients With Heart Failure: Observations From a Developing Country in the Middle East

Introduction: Self-care is recognized as a means for improving outcomes of heart failure (HF), yet studies have not addressed what predicts successful self-care in collectivist cultures like Lebanon. Methodology: Self-care was measured, using the Arabic Self-Care of HF index, in 100 participants with HF (76% males; mean age 67.59) recruited from a tertiary medical center. Results: Self-care was suboptimal, with mean scores of 67.26, 66.96, and 69.5 for self-care maintenance, management, and confidence. Better HF knowledge, social support, and self-care confidence and lower New York Heart Association score predicted better self-care maintenance. Better knowledge, social support, and self-care maintenance, no recent hospitalization, and being unemployed predicted better self-care confidence. Better self-care confidence, maintenance, and HF knowledge predicted better self-care management. Discussion: HF self-care in Lebanon is suboptimal. Nurses need to identify facilitators of and barriers to self-care particular to this population. Interventions targeting HF knowledge, confidence, and caregiver support are expected to improve self-care in Lebanese patients.
The perspectives, barriers, and willingness of Utah dentists to engage in human papillomavirus (HPV) vaccine practices

ABSTRACT Including dental health providers in human papillomavirus (HPV) vaccination could reduce rising rates in HPV-associated oropharyngeal cancer (HPV-OPC). This study assessed Utah dentists’ perspectives on providing HPV vaccination education and services in the dental setting. A cross-sectional, 70-item self-administered survey was conducted among a convenience sample of N = 203 practicing Utah dentists. Statistical analyses included Chi Square tests of independence, scaled scores and Cronbach’s alpha coefficients. Majority of Utah dentists surveyed perceived that discussing the link between HPV and OPC and recommending the HPV vaccine is within their scope of practice, but not administration of the HPV vaccine. Dentists with >10 minutes of patient education per week were less likely to be concerned about the cultural, social norms or religious ideology of discussing HPV with their patients (p = .024). Rural dentists were more concerned about the safety and liability of the HPV vaccine (p = .011). Good internal consistency was observed survey items regarding barriers and willing to engage in HPV vaccination practices. Dental providers were interested in HPV training and patient education brochures as strategies, but less interested in administering the HPV vaccine. Dental associations support dentists’ engagement in HPV education and HPV-OPC prevention. This is the first study in Utah to examine dentists’ perspectives on HPV vaccination. Findings have implications for program planning, intervention development, and future research.
Motivators, Barriers and Other Factors Related to Bean Consumption in Older Adults

Abstract Beans are nutrient-dense and can reduce risk of chronic diseases. This is relevant to older adults who can benefit from consuming beans to reduce their elevated chronic disease risk. This study explored bean consumption in older adults (≥65 years) using mixed-methods including a researcher-administered questionnaire (n = 250) and focus groups (n = 49). Prevalence of bean consumption (daily or weekly) was 51.2%. Motivators to bean consumption were significantly more likely among bean consumers with the top three including nutritional value, taste/texture and versatility, which were also predictors of consuming beans (OR = 3.54, 2.72, and 4.24, respectively). Conversely, barriers to bean consumption were significantly more likely among bean non-consumers with the top three including not part of traditional diet/do not think to include beans in meals, flatulence/abdominal discomfort and lack of knowledge about preparation/cooking, which were also predictors of not consuming beans (OR = 3.85, 2.26, and 5.08, respectively). This research will inform dietary strategies to increase bean consumption.
Lactate dehydrogenase and serum tumor markers for predicting metastatic status in geriatric patients with lung adenocarcinoma.

BACKGROUND No tumor biomarker (TM) is available for de novo metastatic lung adenocarcinoma. OBJECTIVE To examine the serum levels of carcinoembryonic antigen (CEA), cytokeratin-19 fragments (CYFRA21-1), neuron-specific enolase (NSE), carbohydrate antigen (CA) 19-9, CA125, tissue polypeptide antigen (TPA), tissue polypeptide specific antigen (TPS), and lactate dehydrogenase (LDH) to predict de novo metastatic lung adenocarcinoma. METHODS This was a retrospective study of geriatric (> 60 years of age) patients with lung cancer diagnosed at Shanxi Cancer Hospital from 02/2012 to 12/2017. CEA, CYFRA21-1, CA199, NSE, CA125, TPA, and TPS were detected by ELISA and LDH was detected by LDH kit. Their predictive value was assessed using receiver operating characteristic (ROC) curves and multivariable logistic regression. RESULTS The positive rates of LDH and TMs were higher in the metastatic group (all P< 0.05). The best single TMs were CYFRA21-1 (70.5% sensitivity) and CA199 (92.0% specificity). When using any two, the best were CYFRA21-1+TPA (77.1% sensitivity) and CA199+TPA or NSE (both 84.1% specificity). High LDH and CA125 statuses were each independently associated with brain, bone, liver, and lung metastases (all P< 0.05). CONCLUSIONS Abnormal level of LDH and TMs, alone or in combination, had predictive value for metastasis in geriatric patients with lung adenocarcinoma; these indicators were also associated with the metastatic site.
CT-defined Visceral Pleural Invasion in T1 Lung Adenocarcinoma: Lack of Relationship to Disease-Free Survival.

BackgroundPathologic visceral pleural invasion (pVPI) leads to upstaging from T1 to T2. However, it is unclear whether the CT features for pVPI can be reliably used as a clinical T2 descriptor for preoperative staging.PurposeTo validate the diagnostic accuracy and analyze the prognostic value of CT findings for the prediction of pVPI in patients with resected node-negative lung adenocarcinoma.Materials and MethodsThis retrospective cohort study included clinical T1N0M0 adenocarcinomas resected between 2009 and 2015. The diagnostic CT findings suggestive of pVPI were evaluated by a thoracic radiologist. The accuracy of diagnostic CT findings in relation to pVPI and accuracy for disease-free survival (DFS) were evaluated by using test performance metrics and multivariable Cox regression analysis, respectively.ResultsThe authors analyzed 695 patients (median age, 63 years; 411 women). Data for pVPI were not available in six patients. The accuracy of CT features for pVPI ranged from 62.7% (432 of 689 patients) to 72.3% (498 of 689 patients). Positive predictive values ranged from 44.1% (173 of 392 patients) to 56.4% (88 of 156 patients), which indicated that about half of the CT-based predictions were false-positive. Multivariable Cox regression models showed that none of the combinations of CT findings were independent predictors of DFS (adjusted hazard ratios, 1.40, 1.48, 1.06, and 1.21 for each combination; P > .05 for all). In addition, pVPI was not an independent prognostic factor (adjusted hazard ratio, 1.27; P = .26), whereas age and clinical T category were independent prognostic factors in all Cox models (P < .05 for all). ConclusionCT features of pathologic visceral pleural invasion (pVPI) have an accuracy of 62.7%-72.3%. CT features of pVPI were not independent prognostic factors for disease-free survival in clinical T1 lung adenocarcinomas. This argues against the use of CT features of visceral pleural invasion as T2 descriptors in the clinical staging of lung cancer. © RSNA, 2019Online supplemental material is available for this article.See also the editorial by Nishino in this issue.
Prior antibiotic use and acquisition of multidrug-resistant organisms in hospitalized children: A systematic review

Abstract Objective: Multidrug-resistant organisms (MDROs) cause ~5%–10% of infections in hospitalized children, leading to an increased risk of death, prolonged hospitalization, and additional costs. Antibiotic exposure is considered a driving factor of MDRO acquisition; however, consensus regarding the impact of antibiotic factors, especially in children, is lacking. We conducted a systematic review to examine the relationship between antibiotic use and subsequent healthcare-associated infection or colonization with an MDRO in children. Design: Systematic review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guideline. Methods: We searched PubMed and Embase for all English, peer-reviewed original research studies published before September 2018. Included studies evaluated hospitalized children, antibiotic use as an exposure, and bacterial MDRO as an outcome. Results: Of the 535 studies initially identified, 29 met the inclusion criteria. Overall, a positive association was identified in most studies evaluating a specific antibiotic exposure (17 of 21, 81%), duration of antibiotics (9 of 12, 75%), and number of antibiotics received (2 of 3, 67%). Those studies that evaluated any antibiotic exposure had mixed results (5 of 10, 50%). Study sites, populations, and definitions of antibiotic use and MDROs varied widely. Conclusions: Published studies evaluating this relationship are limited and are of mixed quality. Limitations include observation bias in recall of antibiotic exposure, variations in case definitions, and lack of evaluation of antibiotic dosing and appropriateness. Additional studies exploring the impact of antibiotic use and MDRO acquisition may be needed to develop effective antibiotic stewardship programs for hospitalized children.
How to get away with nonsense: Mechanisms and consequences of escape from nonsense‐mediated RNA decay

Nonsense‐mediated RNA decay (NMD) is an evolutionarily conserved RNA quality control process that serves both as a mechanism to eliminate aberrant transcripts carrying premature stop codons, and to regulate expression of some normal transcripts. For a quality control process, NMD exhibits surprising variability in its efficiency across transcripts, cells, tissues, and individuals in both physiological and pathological contexts. Whether an aberrant RNA is spared or degraded, and by what mechanism, could determine the phenotypic outcome of a disease‐causing mutation. Hence, understanding the variability in NMD is not only important for clinical interpretation of genetic variants but also may provide clues to identify novel therapeutic approaches to counter genetic disorders caused by nonsense mutations. Here, we discuss the current knowledge of NMD variability and the mechanisms that allow certain transcripts to escape NMD despite the presence of NMD‐inducing features.
Scale-up production, characterization and toxicity of a freeze-dried lipid-stabilized microbubble formulation for ultrasound imaging and therapy

Abstract Microbubble formulations have a long history for enhancement of ultrasound (US) imaging and recently also for therapeutic applications. Previously, a series of freeze-dried bubble formulations based on the lipids DSPC and DSPG were developed. Here, we have attempted to scale-up the production process for future more extensive studies. Bubbles were prepared by homogenization of a lipid dispersion in a perfluoropropane atmosphere in a medium size (300–500 mL) homogenizer and then freeze-dried for better storage stability. In total, 300 freeze-dried vials were prepared. The properties of the bubbles were similar to those previously prepared on a lab scale with the difference that they were slightly larger and also had a better stability. The re-entrapped gas concentration after re-constituted freeze-dried bubbles was 9.4 µL/µmol lipid. The re-entrapped rate was 72.3% of fresh bubble before freeze-drying (13.0 µL/µmol lipid). The half-life of US imaging signal of the re-constituted freeze-dried bubbles in water in vitro was shorter than that of the fresh bubbles (2.7 min vs. 3.3 min). A leak of Evans Blue, that binds to albumin, from mouse ear blood vessel was observed after combination of bubble and US irradiation of 1 MHz for 1 min. As a result of bubble vibration by US irradiation, vascular endothelial cell bond opened and Evans Blue leaked. Toxicity of bubble was tested in rats. No toxicity was found after a single injection in the dose range tested. No serious toxicity was seen after repeated injections (one daily injection during 15 days).
Children’s and Mothers’ Perspectives of Problematic Eating Behaviours in Young Children and Adolescents: An Exploratory Study

The aim of this study was to (a) compare children’s perspectives of problematic eating behaviours with those of mothers and (b) check if there are differences in the level of these problematic eating behaviours between girls and boys in different age groups (young children: 8–11 years old vs. adolescents: 12–16 years old). The study involved 203 children (50.74% girls) and 203 mothers. The average age of children was 11.06 years (SD = 2.31), and the average BMI was 18.27 kg/m2 (SD = 2.29). Two questionnaires were used to assess children’s perspectives of problematic eating behaviours: The Three-Factor Eating Questionnaire (TFEQ-R13) and the Dutch Eating Behaviour Questionnaire for Children (DEBQ-C). One questionnaire was used to evaluate mothers’ perspectives: The Child Eating Behaviour Questionnaire (CEBQ). The main results in the study indicate the following: (a) the children’s perspective based on the DEBQ-C is the most effective at predicting their BMI (this model of problematic eating behaviours explains 29% of the variance in the child’s BMI); and (b) for almost all problematic eating behaviours, older girls have the highest levels. From the current study, it can be concluded that the type of questionnaire (TFEQ-R13 vs. DEBQ-C vs. CEBQ) and the perspective (child vs. mother) differentiate the results obtained regarding the assessment of children’s problematic eating behaviours and their relation to BMI.
Comparison of Clinical Outcomes After Anatomic Total Shoulder Arthroplasty and Reverse Shoulder Arthroplasty in Patients 70 Years and Older With Glenohumeral Osteoarthritis and an Intact Rotator Cuff.

INTRODUCTION As the indications for reverse shoulder arthroplasty (RSA) expand beyond traditional cuff tear arthropathy, the role of RSA in elderly patients with glenohumeral arthritis and an intact rotator cuff remains unclear. METHODS This retrospective cohort study included 135 patients who underwent RSA or total shoulder arthroplasty (TSA) at a single tertiary orthopedic center between 2005 and 2015 and were 70 years of age or older at the time of surgery. All patients had preoperative advanced imaging confirming an intact rotator cuff but active forward elevation less than 90°. Complications, reoperations, and patient survival were recorded from the medical record. Patient-reported outcomes (Pain visual analog scale, Satisfaction Score, American Shoulder and Elbow Surgeons [ASES], and Western Ontario Osteoarthritis of the Shoulder [WOOS]) and patient-reported range of motion were collected at a minimum of 2 years after procedure. RESULTS There was no significant difference in complication rate or revision surgery rate between patients undergoing TSA and RSA (complications 13.7% versus 12.1%, P = 0.810; reoperations 6.9% vs 3.0%, P = 0.418). There were no differences in patient-reported outcome measures between the two groups. Mean pain visual analog scale scores were low in both groups (0.72, SD 1.93 for TSA and 0.31, SD 0.72 for RSA). Satisfaction scores were high (86.1, SD 23.3 for TSA and 91.8, SD 9.0 for RSA, P = 0.286). Mean ASES and WOOS scores were also high in both groups (86 [SD 15.6] for TSA and 83 [SD 12.6] for RSA for ASES [P = 0.400] and 86 [SD 18.3] for TSA and 89 [SD 10.2] for RSA for WOOS [P = 0.400]). One hundred percent of subjects following RSA and 98% of subjects following TSA rated their forward elevation as full or nearly full (>135°) (P = 0.516). DISCUSSION Given the good clinical outcomes after both TSA and RSA, there may be an increased role for RSA in this elderly cohort to provide effective treatment of glenohumeral osteoarthritis. LEVEL OF EVIDENCE Level III, retrospective comparative study.
A Short Report on the Polymerization of Pyrrole and Its Copolymers by Sonochemical Synthesis of Fluorescent Carbon Dots

In polymer chemistry, polymerization constitutes the process of the conversion of monomers into polymers using an initiator to form polymeric chains. There are many polymerization techniques and different systems exist by which the polymers are classified. Recently, our group has reported the synthesis of polymers using both carbon dots (CDs) and UV light as initiators. In these reports, the carbon dots were used with or without UV light. The CDs produce free radicals in the presence of UV light, indicating their role as initiators. The CD surface has many unshared or unpaired electrons, making it negatively charged. The present study focuses on the use of CDs for the formation of polymers from monomers containing various functional group. The properties of the synthesized CDs and the polymers obtained from the various monomers were characterized by various analytical techniques, including Fourier-Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD), Thermogravimetric Analysis (TGA) and Solid-State NMR spectroscopy. This polymerization technique is of interest both from the scientific aspect and for its applicative potential. The synthesized polymers were investigated for their various applications.
The Effect of Chitosan Derivatives on the Compaction and Tension Generation of the Fibroblast-populated Collagen Matrix

Fibrotic diseases, such as Dupuytren’s contracture (DC), involve excess scar tissue formation. The differentiation of fibroblasts into myofibroblasts is a significant mechanism in DC, as it generates tissue contraction in areas without wound openings, leading to the deposition of scar tissue, and eventually flexing one or more fingers in a restrictive fashion. Additionally, DC has a high recurrence rate. Previously, we showed that N-dihydrogalactochitosan (GC), an immunostimulant, inhibited myofibroblast differentiation in a DC fibroblast culture. Our goal of this study was to expand our previous study to include other DC and normal cell lines and other chitosan derivatives (GC and single-walled carbon nanotube-conjugated GC) to determine the specific mechanism of inhibition. Derivative-incorporated and vehicle control (water) anchored fibroblast-populated collagen matrices (aFPCM) were used to monitor compaction (anchored matrix height reduction) using microscopy and optical coherence tomography (OCT) for six days. Fibroblasts were unable to compact chitosan derivative aFPCM to the same extent as vehicle control aFPCM in repeated experiments. Similarly, chitosan derivative aFPCM contracted less than control aFPCM when released from anchorage. Proliferative myofibroblasts were identified by the presence of alpha smooth muscle actin via myofibroblast proliferative assay. In all tested conditions, a small percentage of myofibroblasts and proliferative cells were present. However, when aFPCM were treated with transforming growth factor-beta 1 (TGF-β1), all tested samples demonstrated increased myofibroblasts, proliferation, compaction, and contraction. Although compaction and contraction were reduced, there was sufficient tension present in the chitosan derivative aFPCM to allow exogenous stimulation of the myofibroblast phenotype.
Direct fluorescent glycan labeling with recombinant sialyltransferases

Abstract Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and detection of N- and O-glycans on glycoproteins. The method allows detection of specific glycans without the laborious gel blotting and chemiluminescence reactions used in Western blotting. The method can also be used with a variety of fluorescent dyes.