Miracle-dz/UniFAR-FTUs huggingface dataset:
Diagnosis and Classification of Malignant Lymphoma and Related Entities in the Bone Marrow Trephine Biopsy

The trephine bone marrow (BM) biopsy is an important diagnostic tool in patients with malignant lymphoma. BM examination can serve to establish or confirm a primary diagnosis of lymphoma or to determine the extent of disease dissemination for staging purposes. BM histology renders information which cannot be gained equally from aspirate material, such as spacial distribution and extent of infiltrates, BM cellularity and fibrosis. Furthermore, cytology including flow cytometric immunophenotyping can give false-negative results in BM involvement by lymphoma due to intralesional fibrosis. In addition to morphological examination, the availability of a broad panel of antibodies suitable for paraffin-embedded tissues, in conjunction with less damaging decalcification procedures, nowadays enables us to perform complete immunophenotyping on BM trephines and allows for classification of lymphoma infiltrates according to established algorithms. Molecular determination of clonality and interphase fluorescent in situ hybridization can be employed selectively to resolve difficult cases. This review describes important diagnostic features of malignant lymphoma in the BM, relevant differential diagnoses, and the proper use of ancillary techniques.
Three consecutive related bone marrow transplants for juvenile myelomonocytic leukaemia

Abstract: Allogeneic haematopoietic stem cell transplantation (allo‐HSCT) is the only cure for juvenile myelomonocytic leukaemia (JMML), but relapse remains the major cause of failure. A second transplant may be considered a way to induce the graft vs. leukaemia effect in patients who relapse after their first HSCT. We describe a 7‐month‐old girl with JMML who relapsed after a first, related allo‐HSCT, and who again relapsed 8 months after the second transplant, despite discontinuation of immusuppressive therapy. She underwent a third allogeneic transplant from another related donor. At the time of this report the patient is in complete remission 26 months after the third transplant. We suggest that a third allo‐HSCT may be taken into consideration for JMML patients who experience relapse, even after two previous transplants.
Analysis and design of a UHF RFID tag antenna with a Split Ring Resonator

In this paper, the UHF RFID tag antenna which has a SRR as a radiating part and inductively coupled feeding method are analyzed and designed. By using a SRR, the antenna size can be reduced and using an inductively coupled feeding method, we can obtain a wide bandwidth and a simple matching technique to directly attach to a tag chip. Also equivalent circuit model of the tag antenna is presented and unknown variables are analyzed and checked. Therefore the tag antenna can be designed for various tag chips and frequency ranges.
Repetitive Transcranial Magnetic Stimulation in Chronic Pain: A Meta-analysis

Background: In this meta-analysis, we explore the role of repetitive transcranial magnetic stimulation (rTMS), a noninvasive neuromodulation technique in the treatment of chronic pain. Methods: Studies comparing rTMS and conventional treatment for chronic pain were searched. The comparison was made for decrease in the pain scores with and without (sham) the use of rTMS after a follow-up interval of 4–8 weeks. All reported pain scores were converted into a common scale ranging from “0” (no pain) to “10” (worst pain). Results: Nine trials with 183 patients in each of the groups were included in the analysis. The decrease in pain scores with rTMS was 1.12 (95% confidence interval [CI] being 1.46–0.78) (fixed effects, I2 = 0%, P < 0.001) and in sham-rTMS was 0.28 (95% CI being 0.49–0.07) (Fixed effects, I2 = 0, P = 0.01). The pooled mean drop in pain scores with rTMS therapy was higher by 0.79 (95% CI being 0.26–1.33) (fixed effects, I2 = 0, P < 0.01). The duration and frequency of rTMS were highly variable across trials. Publication bias was unlikely (Egger's test, X-intercept = 0.13, P = 0.75). Conclusions: Use of rTMS improves the efficacy of conventional medical treatment in chronic pain patients. This treatment is not associated with any direct adverse effects. However, the duration and frequency of rTMS therapy is presently highly variable and needs standardization.
VAP-A binds promiscuously to both v- and tSNAREs.

Proteins that bind to SNAREs may regulate their function. One such protein, VAP-33, was first discovered in Aplysia californica and has two mammalian homologues, VAP-A and VAP-B. VAP-A has been implicated in vesicle targeting to the plasma membrane based on its location in polarized cells and its ability to bind VAMP in vitro. Here, we demonstrate that VAP-A is a widely expressed resident of the ER/Golgi intermediate compartment in COS-7 cells. Moreover, we demonstrate that VAMP-binding and VAP-dimerization require both the N- and C-terminal domains of VAP-A and also that VAP-A binds to a wide range of SNAREs and fusion-related proteins including syntaxin 1A, rbet1, rsec22, alphaSNAP, and NSF. Together, these results suggest that VAP-A is not a regulator of a specific VAMP, but rather may play a more general role in SNARE-mediated vesicle traffic between the ER and Golgi in nonpolarized cells.
The synthesis of nanostructure TiO2 co‐doped with N and Fe and their application for micro‐polluted source water treatment

The doping of titanium dioxide (TiO2) with various metal or nonmetal elements has been considered as an effective strategy to extend the photoactive wavelength region to include visible light. In this paper, TiO2 nanoparticles co‐doped with N and Fe were prepared via a sol–gel route, followed by a heat treatment at elevated temperatures. The co‐doped TiO2 nanoparticles showed a strong visible light absorption and exhibited an enhanced photocatalytic activity for the degradation of methylene blue as compared with pure TiO2 nanoparticles. Polypropylene packings, coated with the prepared N–Fe co‐doped TiO2 nanoparticles, were used as a heterogeneous catalyst for the treatment of micro‐polluted source water. Under the illumination of sunlight, when the water was treated with the coated polypropylene packings, the reduction of CODMn was about 60%, whereas it was just 10% and 30% when the water was treated with pure polypropylene packings and polypropylene packings coated with pure TiO2, respectively. The NH3‐N could also be decreased dramatically on the co‐doped TiO2‐coated polypropylene packings. The results showed that N–Fe co‐doped TiO2‐coated polypropylene packings still had a strong activity after three months of operation.
Filial imprinting in domestic chicks is associated with spine pruning in the associative area, dorsocaudal neostriatum

Juvenile emotionally modulated learning events are fundamental for the normal development of socio‐emotional competence and intellectual capabilities. Filial imprinting in the domestic chick provides a suitable model to investigate the neural mechanisms underlying such juvenile learning events. The forebrain area dorsocaudal neostriatum (Ndc), a multimodal integration area and presumed equivalent to mammalian parietotemporal association cortices, has been shown to be critically involved in this learning process. We investigated whether filial imprinting is associated with changes of synaptic connectivity in the Ndc. Quantitative measurements of spine densities of a large neuron type in the Ndc revealed a massive pruning of spine synapses after filial imprinting. Compared with 7‐day‐old naive control chicks, imprinted chicks displayed significantly lower spine frequencies on all dendritic segments. Since the average length of the dendritic segments did not change during imprinting, these results can be interpreted as a reduction of the absolute number of spine synapses on this neuron type. In a control region, the primary sensory forebrain area ectostriatum, spine density and dendritic length remained unchanged. These results indicate that synaptic pruning may represent a mechanism of selective synaptic reorganization in higher associative forebrain areas as a fundamental feature of juvenile learning events.
Performance of Multi – user MIMO system in the presence of Polarization diversity

Employing Dual Polarized antenna plays a vital role for the future generation MIMO systems due to its advantages such as space effectiveness and robustness. In this paper, we provide Outage probability of Multiuser Dual Polarized antenna in an uplink environment. Specifically, we provide the simulation results of Outage Probability for different varying parameters such as an increase in a number of the user, noise variance, and Cross Polarization Discrimination. Th is parameter is investigated through Monte Carlo simulation.
End-Facet Polishing of Surface Plasmon Waveguides in Lithium Niobate

This paper describes a custom polishing procedure developed to produce high quality end-facets on die having a rather unique cross section. The dies comprise two thin lithium niobate claddings on either side of a thin narrow gold stripe, supported by a silicon substrate. This heterogeneous structure operates as an optical waveguide supporting a long-range surface plasmon-polariton mode, and is fabricated via wafer-bonding and thinning. Optical insertion loss measurements were obtained on a set of diced waveguides before and after end-facet polishing. Polishing reduced the insertion loss of the waveguides by 4.7-9.1 dB, indicating that one as-diced end-facet contributes a scattering loss of 2.35-4.55 dB.
Beans (Phaseolus ssp.) as a Model for Understanding Crop Evolution

Here, we aim to provide a comprehensive and up-to-date overview of the most significant outcomes in the literature regarding the origin of Phaseolus genus, the geographical distribution of the wild species, the domestication process, and the wide spread out of the centers of origin. Phaseolus can be considered as a unique model for the study of crop evolution, and in particular, for an understanding of the convergent phenotypic evolution that occurred under domestication. The almost unique situation that characterizes the Phaseolus genus is that five of its ∼70 species have been domesticated (i.e., Phaseolus vulgaris, P. coccineus, P. dumosus, P. acutifolius, and P. lunatus), and in addition, for P. vulgaris and P. lunatus, the wild forms are distributed in both Mesoamerica and South America, where at least two independent and isolated episodes of domestication occurred. Thus, at least seven independent domestication events occurred, which provides the possibility to unravel the genetic basis of the domestication process not only among species of the same genus, but also between gene pools within the same species. Along with this, other interesting features makes Phaseolus crops very useful in the study of evolution, including: (i) their recent divergence, and the high level of collinearity and synteny among their genomes; (ii) their different breeding systems and life history traits, from annual and autogamous, to perennial and allogamous; and (iii) their adaptation to different environments, not only in their centers of origin, but also out of the Americas, following their introduction and wide spread through different countries. In particular for P. vulgaris this resulted in the breaking of the spatial isolation of the Mesoamerican and Andean gene pools, which allowed spontaneous hybridization, thus increasing of the possibility of novel genotypes and phenotypes. This knowledge that is associated to the genetic resources that have been conserved ex situ and in situ represents a crucial tool in the hands of researchers, to preserve and evaluate this diversity, and at the same time, to identify the genetic basis of adaptation and to develop new improved varieties to tackle the challenges of climate change, and food security and sustainability.
Why Do Cultured Transplanted Myoblasts Die in Vivo? DNA Quantification Shows Enhanced Survival of Donor Male Myoblasts in Host Mice Depleted of CD4+ and CD8+ Cells or NK1.1+ Cells

Overcoming the massive and rapid death of injected donor myoblasts is the primary hurdle for successful myoblast transfer therapy (MTT), designed as a treatment for the lethal childhood myopathy Duchenne muscular dystrophy. The injection of male myoblasts into female host mice and quantification of surviving male DNA using the Y-chromosome-specific (Y1) probe allows the speed and extent of death of donor myoblasts to be determined. Cultured normal C57BL/10Sn male donor myoblasts were injected into untreated normal C57BL/10Sn and dystrophic mdx female host mice and analyzed by slot blots using a 32P-labeled Y1 probe. The amount of male DNA from donor myoblasts showed a remarkable decrease within minutes and by 1 h represented only about 10–18% of the 2.5 × 105 cells originally injected (designated 100%). This declined further over 1 week to approximately 1–4%. The host environment (normal or dystrophic) as well as the extent of passaging in tissue culture (early “P3” or late “P15–20” passage) made no difference to this result. Modulation of the host response by CD4+/CD8+-depleting antibodies administered prior to injection of the cultured myoblasts dramatically enhanced donor myoblast survival in dystrophic mdx hosts (15-fold relative to untreated hosts after 1 week). NK1.1 depletion also dramatically enhanced donor myoblast survival in dystrophic mdx hosts (21-fold after 1 week) compared to untreated hosts. These results provide a strategic approach to enhance donor myoblast survival in clinical trials of MTT.
Role of the dentist in the management of orofacial pain.

Pain is defined by the International Association for the Study of Pain as an unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms as such [1]. Although acute pain has the benefit of protection and healing, chronic pain has less value and can become one of the most powerful distressing experiences of the human spirit. Chronic pain is a major healthcare problem costing the USA approximately US$635 billion each year in medical treatment and lost productivity [2]. In a cross-sectional internet-based survey of more than 35,000 individuals, the prevalence of chronic pain, defined as chronic, recurrent or long-lasting pain, lasting for at least 6 months, was 30.7%. The prevalence was higher for females (34.3%) than males (26.7%) and increased with age. Half of respondents with chronic pain experienced daily pain and 32% of them reported their average pain intensity as severe (≥7 on a scale ranging from 0 to 10) [3]. In a screening interview in 15 European countries and Israel, 19% of 46,394 respondents reported suffering with pain for 6 months or longer and had experienced pain in the last month and several times during the last week. Of these respondents, 46% reported their pain as constant [4]. In another study, 90% of the individuals with chronic pain localized their pain to the musculoskeletal structures [5]. Similar findings are reported when only the orofacial regions are considered. Macfarlane et al. [6] found the overall prevalence of orofacial pain (OFP) in the general population to be 26%. According to specific anatomic sites, this study found the prevalence of pain complaints as follows: 6% pain in the jaw joints, 6% in front of the ears, 10% in and around the temples and 12% in the periorbital region. Professional consultation with a dentist or physician was only sought by 46% of the participants with OFP. Furthermore, 17% reported work absenteeism or were disabled from undertaking normal activities because of pain. In another survey of 45,711 American households, 22% of the respondents reported having experienced at least one of five types of OFP in the past 6 months. The most common was tooth pain at 12.2% followed by t emporomandibular joint pain in 5.3% [7].
EVALUATING AND INTEGRATING THE MCKNIGHT’S TRUST RELATED MODELS

This paper primarily aims to develop a comprehensive model of initial trust by evaluating and integrating antecedents of interpersonal trust within the McKnight’s trust related models. We have firstly identified antecedents of interpersonal trustby reviewed the literature and subsequently classified them by evaluating the properties of the constructs. Based on the review, seven constructsrelated to antecedents of interpersonal trust have partly been identified toincorporatein and extend the McKnight’s trust models. They are: (1) situation normality, (2) structural assurance, (3) disposition to trust, (4) perceived site quality, (5) perceived vendor reputation, (6) co-brand image, (7) co-brand awareness. This study contributes academically by providing a comprehensive conceptual model, where these essential constructs are assembled together. Future empirical validation is suggested at the end of this paper.
A micromirror device with post-fabrication re-adjustable pull-in parameters

In prevalent torsion actuators the angular travel range and pull-in instability are set and cannot be changed once the device is fabricated. The current work presents a novel adjustable micromirror device. By using multiple electrodes, the novel design allows continuous and reversible adjustment of the electromechanical response and pull-in instability of a fabricated device. This response adjustment is demonstrated on a torsion micromirror device and it is used for analog adjustment of the stable angular travel range of micromirror. In addition, the inherent properties of the device enable simple duty cycle modulation of the dynamic response that can be used for gray-scale control.
H2A.X. a histone isoprotein with a conserved C-terminal sequence, is encoded by a novel mRNA with both DNA replication type and polyA 3' processing signals.

A full length cDNA clone that directs the in vitro synthesis of human histone H2A isoprotein H2A.X has been isolated and sequenced. H2A.X contains 142 amino acid residues, 13 more than human H2A.1. The sequence of the first 120 residues of H2A.X is almost identical to that of human H2A.1. The sequence of the carboxy-terminal 22 residues of H2A.X is unrelated to any known sequence in vertebrate histone H2A; however, it contains a sequence homologous with those of several lower eukaryotes. This homology centers on the carboxy-terminal tetrapeptide which in H2A.X is SerGlnGluTyr. Homologous sequences are found in H2As of three types of yeasts, in Tetrahymena and Drosophila. Seven of the nine carboxy-terminal amino acids of H2A.X are identical with those of S. cerevisiae H2A.1. It is suggested that this H2A carboxy-terminal motif may be present in all eukaryotes. The H2A.X cDNA is 1585 bases long followed by a polyA tail. There are 73 nucleotides in the 5' UTR, 432 in the coding region, and 1080 in the 3' UTR. Even though H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail, its mRNA also contains the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. Two forms of H2A.X mRNA, consistent with the two sets of processing signals were found in proliferating cell cultures. One, about 1600 bases long, contains polyA; the other, about 575 bases long, lacks polyA. The short form behaves as a replication type histone mRNA, decreasing in amount when cell cultures are incubated with inhibitors of DNA synthesis, while the longer behaves as a basal type histone mRNA.
Dual-band RFID tag antenna with EBG for glass objects

The dual-band tag antenna designed for glass objects of HF-RFID (13.56 MHz) and UHF-RFID (920-925 MHz) applications along national standard of Thailand is proposed. The tag antenna has the same size with a credit card. The overall dimension of the tag antenna with EBG is 147 mm × 73.5 mm × 8.3 mm. The purpose of this antenna design is to implement with the RFID for the highway tracking and tolls systems. The tag antenna and EBG are designed on FR-4 substrate with thickness of 0.3 mm and 3 mm, respectively. To achieve the dual band operation, the designed tag antenna with EBG structure is based on the loop (spiral coil), the meander-line structure with double T-matching and mushroom-like EBG structure for HF-, UHF-RFID and EBG, respectively.
Engineering intracellular active transport systems as in vivo biomolecular tools.

Active transport systems provide essential functions in terms of cell physiology and metastasis. These systems, however, are also co-opted by invading viruses, enabling directed transport of the virus to and from the cell's nucleus (i.e., the site of virus replication). Based on this concept, fundamentally new approaches for interrogating and manipulating the inner workings of living cells may be achievable by co-opting Nature's active transport systems as an in vivo biomolecular tool. The overall goal of this project was to investigate the ability to engineer kinesin-based transport systems for in vivo applications, specifically the collection of effector proteins (e.g., transcriptional regulators) within single cells. In the first part of this project, a chimeric fusion protein consisting of kinesin and a single chain variable fragment (scFv) of an antibody was successfully produced through a recombinant expression system. The kinesin-scFv retained both catalytic and antigenic functionality, enabling selective capture and transport of target antigens. The incorporation of a rabbit IgG-specific scFv into the kinesin established a generalized system for functionalizing kinesin with a wide range of target-selective antibodies raised in rabbits. The second objective was to develop methods of isolating the intact microtubule network from live cells as a platform for evaluatingmore » kinesin-based transport within the cytoskeletal architecture of a cell. Successful isolation of intact microtubule networks from two distinct cell types was demonstrated using glutaraldehyde and methanol fixation methods. This work provides a platform for inferring the ability of kinesin-scFv to function in vivo, and may also serve as a three-dimensional scaffold for evaluating and exploiting kinesin-based transport for nanotechnological applications. Overall, the technology developed in this project represents a first-step in engineering active transport system for in vivo applications. Further development could potentially enable selective capture of intracellular antigens, targeted delivery of therapeutic agents, or disruption of the transport systems and consequently the infection and pathogenesis cycle of biothreat agents. « less