Miracle-dz/UniFAR-FTUs huggingface dataset:
Human Erythropoietin Induces a Pro-Angiogenic Phenotype in Cultured Endothelial Cells and Stimulates Neovascularization In Vivo

Hematopoietic and endothelial cell lineages share common progenitors. Accordingly, cytokines formerly thought to be specific for the hematopoietic system have been shown to affect several functions in endothelial cells, including angiogenesis. In this study, we investigated the angiogenic potential of erythropoietin (Epo), the main hormone regulating proliferation, differentiation, and survival of erythroid cells. Epo receptors (EpoRs) have been identified in the human EA.hy926 endothelial cell line by Western blot analysis. Also, recombinant human Epo (rHuEpo) stimulates Janus Kinase-2 (JAK-2) phosphorylation, cell proliferation, and matrix metalloproteinase-2 (MMP-2) production in EA.hy926 cells and significantly enhances their differentiation into vascular structures when seeded on Matrigel. In vivo, rHuEpo induces a potent angiogenic response in the chick embryo chorioallantoic membrane (CAM). Accordingly, endothelial cells of the CAM vasculature express EpoRs, as shown by immunostaining with an anti-EpoR antibody. The angiogenic response of CAM blood vessels to rHuEpo was comparable to that elicited by the prototypic angiogenic cytokine basic fibroblast growth factor (FGF2), it occurred in the absence of a significant mononuclear cell infiltrate, and it was not mimicked by endothelin-1 (ET-1) treatment. Taken together, these data demonstrate the ability of Epo to interact directly with endothelial cells and to elicit an angiogenic response in vitro and in vivo and thus act as a bona fide direct angiogenic factor.
SUPPORT FOR THE SPONDYLITIC SPINE BY MEANS OF BURIED STEEL BARS, ATTACHED TO THE VERTEBRÆ

Fritz Lange (1864-1952) was born in Dessau and studied medicine in several of the major German universities. He finished his orthopedic training in Strassburg. In 1896 he moved into Munich, where he developed an orthopedic clinic that gained state support and became the leading orthopedic and rehabilitation center for Bavaria. His work led to the establishment of a chair of orthopedics at the University of Munich, to which he was appointed. Seven of his students gained important positions in German universities. In 1910 he was invited by Augustus Thorndike, the president, to address the annual meeting of the American Orthopaedic Association, which was held in Washington, D.C. Lange’s paper, presented on that occasion, is reproduced here.
Modelización espacial de huevos de Thrips(Thysanoptera: Frankliniella occidentalis) en tomate de cáscara por medio de técnicas geoestadísticas

El tomate de cascara ( Physalis ixocarpa Brot.) es un cultivo alimenticio de gran importancia economica en Mexico. Sin embargo, es afectado por diversas plagas y enfermedades tales como los Thrips (Thysanoptera: Frankliniella occidentalis ) y el virus de la marchitez manchada del tomate (TSWV) que llegan a causar hasta un 80% de perdidas. El objetivo del presente trabajo fue modelizar la distribucion espacial de huevos de Thrips mediante tecnicas geoestadisticas y obtener, en consecuencia, mapas de incidencia por medio del Kriging. Se georreferenciaron 121 puntos de muestreo en cada una de las parcelas comerciales de los municipios de Luvianos, Jocotitlan e Ixtlahuaca, a traves del metodo de transectos en tres etapas fenologicas del cultivo. Se contabilizo el numero de huevos de Thrips en cada punto de muestreo. Los resultados mostraron que las poblaciones de huevos de Thrips presentan una distribucion agregada, identificandose varios centros de conglomeracion a traves de los mapas obtenidos. Los semivariogramas obtenidos de la distribucion espacial se ajustaron principalmente a los modelos gaussianos y esfericos. La distribucion de huevos de Thrips se presento en centros de agregacion dentro de las parcelas estudiadas, lo cual permitira establecer estrategias y medidas de control o mitigacion en terminos de sitios especificos de infestacion de huevos de Thrips .
Does the Cerebrospinal Fluid Reflect Altered Redox State But Not Neurotrophic Support Loss in Parkinson's Disease?

Abstract Alteration in neurotrophic factors support and antioxidant defenses in the central nervous system (CNS) along with deficit of ferritin have been associated with idiopathic Parkinson's disease (PD). The objectives were to analyze in the cerebrospinal fluid (CSF) of patients with PD and controls the following: (i) the levels of the neuroprotectant factors glial cell line-derived neurotrophic factor, persephin, neurturin, and brain-derived neurotrophic factor, (ii) the levels of transforming growth factor-β1 (TGFβ1) and transforming growth factor-β2 (TGFβ2), proinflammatory factors, (iii) the activity of the antioxidant enzymes glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-S-transferase (GST), catalase, superoxide dismutases (SODs), and peroxiredoxins (PRDxs), and (iv) ferritin levels. The study revealed that, among neurotrophic factors, only TGFβ1 levels were found to be enhanced in patients with PD (early, p < 0.05; advanced, p < 0.02). Regarding antioxidant enzymes, the ac.
A two dimensional comparative study of the soft tissue profile of a group of post operative orthognathic patients to a control group

Orthognathic surgery aims to correct underlying dentofacial deformities and improve facial aesthetics. This study was designed to compare the two-dimensional (2D) lateral facial soft tissue profiles of a group of post-surgical patients (orthognathic group) to a control group of individuals recruited from the local population in the West of Scotland. The relative attractiveness of 112 volunteers (61 females, 51 males), recruited from the local population and aged 18 to 35 years, were rated by a lay panel (four males, four females) who assessed three dimensional (3D) facial images of the volunteers using a Visual Analogue Scale. 16 males and 24 females, rated as being “attractive” and “most attractive” were selected to form the control group. The orthognathic group of 33 patients (17 females, 16 males) was recruited from the Dentofacial Deformity Clinic based at the Glasgow Dental Hospital and the Southern General Hospital, Glasgow. Right lateral 2D facial profile photographs of the control group and the orthognathic group were taken, and digital identification of soft tissue facial lateral profile landmarks completed. Outcome measures were angular, linear horizontal and vertical linear measurements taken from the soft tissue landmarks. Comparison of control males to control females showed that the males had longer faces and more prominent chins than the females. The male orthognathic group had more protrusive lips and chins compared to the male control group, but overall had a similar facial morphology. The female orthognathic group had smaller nasiolabial angles, a longer mid- and lower facial heights and lips and chins which were more prominent than the female control group.
Portal hipertansiyonun intestinal bariyer üzerine etkisi Effect of portal hypertension on intestinal barrier

Background and aims: Portal hypertension leads to considerable hemodynamic changes in the splanchnic circulation. Although nitric oxide may enhance functional mucosal continuity as a potent mediator, it may produce a paradoxical influence. The aim of the study was to assess the histopathologic changes in the digestive tract and evaluate the potential influence of portal hypertension on bacterial translocation together with the role of nitric oxide. Materials and methods: Twenty male Wistar rats used for the study were randomized into two groups. Portal vein constriction was performed in the first group while the second underwent Sham operation. Twelve weeks later portal venous pressures were registered. Intestinal samples were taken for nitric oxide levels and histological examinations. Liver, spleen, and mesenteric lymph node complex were harvested for cultures. Results: Portal venous pressures (22.7±5.7 cmH2O) were significantly higher in the constriction group compared with the sham operation group (10.4±1.5 cmH2O) (p0.05). Conclusions: Increased portal venous pressure was obtained with constriction. Intestinal barrier was not significantly altered in our groups.
Pomegranate (Punica granatum)

Pomegranate (Punica granatum) is an edible fruit originating in the Middle East, the juice of which is widely available commercially. Various parts of the fruit including the juice have been used traditionally to treat a range of ailments. The juice and other extracts have shown a wide range of bioactivity in pre-clinical studies, such as anti-inflammatory, anti-infective and anti-oxidant effects. On the basis of this research, beneficial effects are claimed in cancer, specifically in the prevention and treatment of prostate cancer. Only two trials in human have been conducted to date: one an uncontrolled trial and the other a randomised controlled trial. Both were in patients with prostate cancer and both reported increased prostate specific antigen (PSA) doubling time. Pomegranate juice has been widely consumed for many years and has been used in studies lasting up to 3 years and appears to be safe. A small number of cases of allergic reactions and possible interaction with warfarin have been reported. Limited safety data is available on extracts. There is insufficient evidence from clinical studies to draw conclusions about possible effectiveness in cancer but there do not appear to be any serious concerns as to its safety.
Estudo exploratório descritivo do emprego de coelhos brancos da Nova Zelândia (Orytolagus cuniculus) como modelo animal na avaliação dos padrões de crescimento craniofacial

Os coelhos brancos da Nova Zelândia sao frequentemente relatados como o modelo animal selecionado para experimentos visando avaliacao do crescimento craniofacial. Contudo, existe uma dificuldade de acesso as informacoes referentes aos cuidados no emprego destes especimes durante trabalhos experimentais. Este estudo exploratorio pretende rever a literatura, no que refere-se a selecao de um modelo animal e experimentos que envolvem o crescimento craniofacial bem como relatar os eventos de sucesso e insucesso vividos pelos pesquisadores dentro de um primeiro trabalho experimental, com um modelo animal, numa linha de pesquisa sobre o crescimento craniofacial. Laparos (Oryctolagus cunicullus), em tenra idade, foram manejados dentro de uma rotina de Bioterio, para um correto preparo visando a realizacao de uma cirurgia (experimento). Detalhes referentes ao transporte, criacao, fisiologia, comportamento, acomodacao, anestesia, morte e manejo de doencas sao discutidos para que futuros trabalhos com o proposito de utilizacao deste mesmo modelo animal possam ser abreviados no seu tempo inicial de contato e reconhecimento das peculiaridades desta especie. (AU)
Interferon-γ–induced membrane PAF-receptor expression confers tumor cell susceptibility to NK perforin-dependent lysis

Perforin is known to display a membranolytic activity on tumor cells. Nevertheless, perforin release during natural killer (NK)–cell activation is not sufficient to induce membrane target-cell damage. On the basis of the ability of perforin to interact with phospholipids containing a choline phosphate headgroup, we identify the platelet-activating factor (PAF) and its membrane receptor as crucial components in tumor cell killing activity of human resting NK cells. We demonstrate for the first time that upon activation, naive NK cells release the choline phosphate–containing lysolipid PAF, which binds to perforin and acts as an agonist on perforin-induced membrane damage. PAF is known to incorporate cell membranes using a specific receptor. Here we show that interferon-γ (IFN–γ) secreted from activated NK cells ends in PAF-receptor expression on perforin-sensitive K562 cells but not on perforin-resistant Daudi cells. In order to prove the capacity of PAF to interact simultaneously with its membrane PAF receptor and with perforin, we successfully co-purified the 3 components in the presence of bridging PAF molecules. The functional activity of this complex was further examined. The aim was to determine whether membrane PAF-receptor expression on tumor cells, driven to express this receptor, could render them sensitive to the perforin lytic pathway. The results confirmed that transfection of the PAF-receptor complementary DNA into major histocompatibility complex class I and Fas-receptor negative tumor cells restored susceptibility to naive NK cells and perforin attack. Failure of IFN-γ to induce membrane PAF receptor constitutes the first described mechanism for tumor cells to resist the perforin lytic pathway.
Substituição do milho por farelo de trigo ou farelo de glúten de milho na ração de bovinos de corte em terminação

The objective of this work was to evaluate the substitution of finely ground (GC) corn by wheat middlings (WM) or dried corn gluten feed (CGF) in finishing feedlot diets. Thirty-six yearling bulls (382 kg average initial live weight; 14 months old) were fed during 87 days. The diets contained 24% grass silage and 76% concentrate, formulated with dried citrus pulp, soybean meal, urea, mineral-vitamin mix, sodium bicarbonate, sodium monensin, and one of the energy sources tested. The substitution of wheat middlings for corn increased NDF intake (p<0.05). However, no effect was observed on other measured parameters. Substitution of corn gluten feed for ground corn reduced dry matter intake (p<0.05) and average daily gain (p<0.10), increased NDF intake (p<0.05), but had no effect on feed efficiency. These diets showed no differences in the observed values of net energy of maintenance or gain. The ratio observed/expected net energy for the diet differs from the other treatments. For this type of diet, GC may be completely replaced by WM or CGF with no depression on feed efficiency or on diet net energy. NRC (2001) underestimates TDN values and net energy estimates for WM and CGF.
Secreted phospholipase A2 induces vascular endothelial cell migration

Secreted phospholipase A2 (sPLA2) regulates a variety of cellular functions. The present investigation was undertaken to elucidate the potential role of sPLA2 in endothelial cell (EC) migration. Bovine aortic endothelial cells (BAECs) exposed to sPLA2 placed in the lower compartment of a modified Boyden chamber displayed increased migration compared to cells exposed to vehicle. The effect of sPLA2 on EC migration was time and dose dependent. Migration of BAECs was observed at 30 minutes, increased over 1 to 2 hours, and declined thereafter. At 2 hours of stimulation, sPLA2 (0.01-2 μmol/L) induced 1.2- to 3-fold increased cell migration compared with media alone. Among the different sPLA2s tested, bee venom, Naja naja, and porcine and human pancreatic PLA2s all evoked a migratory response in ECs. Moreover, human synovial fluid, obtained from patients with arthritis and containing sPLA2 activity, induced EC migration. Migration of ECs was significantly reduced after exposure to a catalytic site mutant of pancreatic sPLA2with decreased lipolytic activity as compared to wild-type sPLA2. Similarly, pretreatment of human synovial fluid with p -bromophenacyl bromide, an irreversible inhibitor of sPLA2, markedly decreased the ability of human synovial fluid to stimulate EC migration. Moreover, migration of ECs was stimulated on exposure to hydrolytic products of sPLA2activity including arachidonic acid, lysophosphatidic acid, and lysophosphatidylcholine. These findings suggest that sPLA2plays a physiologic role in induction of EC migration. Moreover, the effects of sPLA2 on EC migration are mediated, at least in part, by its catalytic activity.
ESTABILIDADE DA FITASE DE Aspergillus niger 11T53A9 AO ARMAZENAMENTO E SUA APLICAÇÃO NA HIDRÓLISE DO ÁCIDO FÍTICO NA FARINHA DE SORGO

O acido fitico ou fitato e considerado fator antinutricional por complexar-se aos outros minerais tais como ferro, zinco, calcio, magnesio, no trato gastrointestinal, tornando- os menos disponiveis para a absorcao. O presente trabalho teve por objetivos, avaliar a estabilidade da fitase de Aspergillus niger 11T53A9, submetida as temperaturas de aplicacao industrial (50° C) e, durante o armazenamento sob refrigeracao (3,6°C) e congelamento (-16° Alem disso, a acao da C). fitase fungica na hidrolise do acido fitico presente na farinha de sorgo foi comparada a atuacao de uma fitase comercial de A. niger. O extrato bruto foi obtido a partir da fermentacao em estado solido por A. niger 11T53A9, em condicoes previamente padronizadas. A fitase se manteve estavel a 3,6° C e -16° C durante 6 meses. Na temperatura de 50° manteve 86% de sua C, atividade apos 24 horas. O tratamento enzimatico da farinha de sorgo com 400 e 800U/kg da fitase de A. niger 11T53A9 foi capaz de hidrolisar 88 e 93% do acido fitico presente na farinha de sorgo, reduzindo-o drasticamente nos tempos de 4 e 3 horas, respectivamente, enquanto que o efeito da enzima comercial, nas mesmas concentracoes, foi de 57,6 e 59,4% em 6 horas de hidrolise. Nas condicoes estudadas, a fitase de A. niger 11T53A9, obtida neste trabalho, teve maior desempenho que a fitase comercial (EC 3.1.3.8) para a farinha de sorgo.
5-lipoxygenase expression in dendritic cells generated from CD34+ hematopoietic progenitors and in lymphoid organs

The 5-lipoxygenase (5-LO) pathway in human CD34+ hematopoietic progenitor cells, which were induced to differentiate into dendritic cells (DCs) by cytokines in vitro and in DCs of lymphoid tissues in situ, was examined. Extracts prepared from HPCs contained low levels of 5-LO or 5-LO–activating protein. Granulocyte-macrophage colony-stimulating factor (GM-CSF) plus tumor necrosis factor–α (TNF-α) promoted DC differentiation and induced a strong rise in 5-LO and FLAP expression. Fluorescence-activated cell sorter (FACS) analyses identified a major DC population coexpressing human leukocyte antigen (HLA)-DR/CD80 and monocytic or Langerhans cell markers. Transforming growth factor–β1 (TGF-β–1), added to support DC maturation, strongly promoted the appearance of CD1a+/Lag+ Langerhans-type cells as well as mature CD83+ DCs. TGF-β–1 further increased 5-LO and FLAP expression, recruited additional cells into the 5-LO+DC population, and promoted production of 5-hydroxyeicosatetraenoic acid and leukotriene B4 in response to calcium (Ca++) ionophore [A23187][1]. These in vitro findings were corroborated by 5-LO expression in distinct DC phenotypes in vivo. Scattered 5-LO and FLAP in situ hybridization signals were recorded in cells of paracortical T-lymphocyte–rich areas and germinal centers (GCs) of lymph nodes (LNs) and tonsil and in cells of mucosae overlying the Waldeyer tonsillar ring. 5-LO protein localized to both CD1a+ immature DCs and to CD83+ mature interdigitating DCs of T-lymphocyte–rich areas of LNs and tonsil. As DCs have the unique ability to initiate naive lymphocyte activation, our data support the hypothesis that leukotrienes act at proximal steps of adaptive immune responses. [1]: /lookup/external-ref? link_type=GEN&access_num=A23187&atom=%2Fbloodjournal%2F96%2F12%2F3857.atom
Transcriptional activators in hepatocytes.

A longstanding quest of developmental biology has been to understand the molecular switches that determine which sets of genes are expressed in selected differentiated cell types. Since regulation of gene expression is predominantly at the level of mRNA synthesis (reviewed in Ref. 1), the problem initially becomes one of defining the protein factors that establish and maintain a gene in a transcriptionally active state within the appropriate cell lineage. In mammalian systems, dissection of gene regulatory pathways using the power of genetic selections to identify regulatory proteins remains a largely elusive goal. Consequently, much of our current knowledge of such processes in complex eukaryotes is derived from biochemical characterization of transcriptional activator proteins, especially those that target specific genes by complexing with defined DNA elements located in the vicinity of the transcription unit (2). The mammalian hepatocyte represents a facile and widely exploited system for the investigation of tissuespecific transcription, owing mainly to the large diversity of genes whose expression is restricted to the liver, but also due to the amenability of liver tissue to biochemical experimentation. Liver gene transcription, as with other tissue-specific genes, depends on regulatory sequences situated close to the mRNA start site (i.e., within several hundred bp2 upstream), as well as those found in more distant locations (so-called enhancers). Both types of elements can contribute to the hepatocyte-restricted properties of gene transcription (see, for example, Refs. 3-8). Thus far, the proximal sequences and their binding proteins have been the more thoroughly investigated, a fact that is reflected in the subject matter for this review. This bias should not be interpreted to indicate that the long-range enhancers are less important than proximal signals in establishing histotypic patterns of gene transcription. The intent of this article is to summarize some of the emergent information on liver-specific transcriptional activator proteins. Due to limitations of space, no attempt has been made to prepare a comprehensive
ANTICORPOS CONTRA Leptospira spp EM CERVOS-DO-PANTANAL (Blastocerus dichotomus) NA BACIA DO RIO PARANÁ, ESTADOS DE SÃO PAULO E MATOGROSSO DO SUL, BRASIL

Objetivou-se verificar a ocorrencia de anticorpos contra Leptospira e avaliar espacialmente a dispersao de cervos-do-pantanal (Blastocerus dichotomus) de vida livre, que fossem reagentes, na bacia do Rio Parana, estados de Sao Paulo e Mato Grosso do Sul. Foram examinadas, por meio do teste de soroaglutinacao microscopica (SAM), 217 amostras de soro sanguineo (77 machos e 140 femeas) de cervos-do-pantanal capturados em seis localidades na bacia do rio Parana, entre os estados de Sao Paulo e Mato Grosso do Sul. Desse total, 130 (59,91%) amostras foram reagentes contra 12 diferentes sorovariedades de leptospiras patogenicas e 87 (40,09%) foram nao reagentes. As sorovariedades mais encontradas foram: Autumnalis (20,28% dos animais examinados), Castellonis (13,36%) e Hardjo (11,98%). Os titulos sorologicos obtidos nas amostras reagentes variaram de 100 a 800. Com relacao ao sexo, 52 (67,5%) machos e 78 (55,7%) femeas foram reagentes, nao sendo observada diferenca estatisticamente significativa (P>0,05). A comparacao entre os animais jovens e adultos sororreagentes revelou que houve diferenca significativa (P<0,01) com relacao a idade; 65,1% dos adultos e 34,9% dos jovens foram reagentes. Quanto ao local de captura, observou-se que as frequencias de reagentes diferiram significativamente. A dispersao espacial, obtida por imagens de satelite aliadas as informacoes do geoposicionamento de cada cervo-do-pantanal capturado, mostrou distribuicao de cervos-do-pantanal reagentes na area estudada.
THE EFFICACY OF RHEUM RIBES L. STALK EXTRACT ON LIPID PROFILE IN HYPERCHOLESTEROLEMIC TYPE II DIABETIC PATIENTS: A RANDOMIZED, DOUBLE-BLIND, PLACEBO - CONTROLLED, CLINICAL TRIAL

1 رايداتسا هورگ ،شهوژپ ،يسانشوراد يهاگشنادداهج ييوراد ناهايگ هدكشهوژپ 2 رايداتسا ، نارهت يكشزپ مولع هاگشناد ،مسيلوباتم و ددغ تاقيقحت زكرم 3 داتسا ،راي يكشزپ مولع هاگشناد شترا 4 رايداتسا ، آ و نامرد ،تشادهب ترازو يوراد و اذغ يهاگشيامزآ تاقيقحت زكرم هدكشهوژپ و يكشزپ شزوم يهاگشنادداهج ييوراد ناهايگ 5 نارهت يكشزپ مولع هاگشناد ،ييوراد ناهايگ تاقيقحت زكرم ،يزاسوراد هدكشناد ،يزونگوكامراف هورگ ،رايداتسا 6 مويرابره ،شهوژپ يبرم يهاگشنادداهج ييوراد ناهايگ هدكشهوژپ 7 داتسا ، ناد ،مسيلوباتم و ددغ تاقيقحت زكرم نارهت يكشزپ مولع هاگش * هبتاكم سردآ : ،مجنپ هقبط ،يتعيرش رتكد ناتسراميب ،يلامش رگراك نابايخ هاگشناد مسيلوباتم و ددغ تاقيقحت زكرم نارهت يكشزپ مولع . نفلت : 88220037 كينورتكلا تسپ : rheshmat@razi.tums. ac.ir