Miracle-dz/UniFAR-FTUs huggingface dataset:
Dynamic Microscopy: Reconstructing a Novel Lysosomal Trafficking Pathway

The delivery of newly synthesized lysosomal proteins to endosomes and lysosomes is dependent on several functionally distinct compartments, i.e., the endoplasmic reticulum (ER), the Golgi apparatus and small carrier vesicles. These compartments also play a role in the synthesis, post-translational modification, sorting and distribution of proteins to other destinations. In fact, most cargo is sorted within, and exits from, the trans-Golgi network (TGN). Proteins delivered to the endosomal/lysosomal system include a large and diverse class of hydrolytic enzymes and non-enzymatic activator proteins that were thought to be directed to the lysosomes by their binding to mannose-6-phosphate receptors (MPRs). Surprisingly, in I-cell disease, in which the MPR pathway is disrupted, the non-enzymatic sphingolipid activator protein, prosaposin, continues to traffic to lysosomes. The use of dynamic immunoconfocal and immunoelectron microscopy along with mutational analysis and chimeric protein technology led us to the discovery of a new lysosomal sorting receptor, sortilin. We found that prosaposin is targeted to the lysosomes through an interaction with sortilin. Deletion of the Cterminus of prosaposin abolished its transport to the lysosomes, and mutational analysis revealed that the first half of the prosaposin C-terminus (aa524-540) contains a motif required for its binding to sortilin and its transport to lysosomes. Additionally, a chimeric construct consisting of albumin and a distal segment of prosaposin, which includes its Cterminus, resulted in the routing of albumin to lysosomes. In conclusion, we have identified a specific region in the Cterminus of prosaposin, as well as amino acid residues that are critical for the binding of prosaposin to sortilin and its subsequent lysosomal trafficking.
Testimonies in Exile: Sudanese Gender Politics

Such totalizing of women's activism is being subverted by the more nuanced research being done by a new generation of Sudanese women scholars and others. At the risk of continuing to overgeneralize, I would like to categorize women's activism in Sudan into at least three broad types of movements/mobilizations or associations. One is represented by the sec- ular left and consists of the WU, the Sudan People's Liberation Movement (SPLM), the Sudan Alliance Forces (SAF), and the Sudanese Women's Rights Organization, one of the splinter groups that broke away from the WU. A second type comprises the cultural nationalists/religionists, including, among others, the women's wings of the Umma Party, the
Synthesis of Ferrocene Based Organometallic Compounds & Antimicrobial Activity

The synthesis, characterisation, spectral studies, antibacterial and fugal studies of some transition metal ions like Mn ++ , Co ++ , Ni ++ , Cu ++ , Pd ++ and Pt ++ complexes of ferrocene based Organometallic compounds have been reported. The coordinate bonding of nitrogen to the central metal atom which reduces the electron density in the both azomethine link and thus lower the -HC=N- absorption. The elemental analysis data exhibit the formation of 1:1 (M: L) ratio with coordination number six in the all complexes. The complexes have with octahedral geometry and D4h symmetry. The ferrocene based organometallic compound shows a molecular ion peak at m/z 482.4 which is equal to theoretical molecular weight. The molar conductance value indicates that, they are non- electrolytic in nature. These complexes were tested with some bacteria and fungi.
COOPERAÇÃO CORPORIFICADA: UM ESTUDO DA LINGUAGEM EM USO

Cooperacao e um termo frequente em inumeras discussoes relacionadas ao universo organizacional. Mesmo quando nao e explicitamente evidenciado, e possivel presumi-lo como intrinseco a conceitos evocados por atuais modelos de gestao. Contudo, ao notar tais usos do tema, observa-se um tratamento pouco preocupado com as caracteristicas sociocognitivas envolvidas no comportamento cooperativo. Em contrapartida, a linguistica contemporânea, em interface com a psicologia cognitiva e com a filosofia da linguagem, oferece uma visao bem consolidada sobre o tema em questao: a cooperacao. Coube a pragmatica, disciplina linguistica voltada ao estudo da linguagem em uso, o papel de ponderar tanto sobre os mecanismos psicologicoscognitivos quanto sobre os dispositivos sociais envolvidos na efetivacao da significacao linguistica em atos concretos de comunicacao. Este trabalho estuda como o conceito de cooperacao e apresentado, relacionando-o com areas de conhecimento como as de gestao de pessoas, ciencias cognitivas, lingui ica teorica e filosofia da linguagem.
Algorithmes non paramétriques pour la synthèse de textures volumiques à partir d'un exemple 2D

Cet article traite de synthese non parametrique de textures volumiques anisotropes a partir d'une observation 2D unique. Nous proposons differentes variantes originales d'un algorithme non parametrique et multiechelle de synthese de textures. L'originalite reside d'une part dans le fait que le processus de synthese 3D s'appuie sur l'echantillonnage d'une seule image 2D d'entree en garantissant la coherence selon deux des trois vues de la texture 3D de sortie et, d'autre part, dans les strategies de parcours 3D et d'optimisation utilisees. Les differentes variantes algorithmiques proposees sont appliquees a la synthese de textures volumiques de materiaux carbones, a partir d'une observation 2D unique obtenue par Microscopie Electronique en Transmission (MET). Nous developpons une etude qualitative et quantitative des resultats de synthese qui permet d'une part d'identifier les strategies les plus pertinentes pour la synthese et d'autre part de les comparer de facon objective a certains algorithmes de la litterature.
The HLA-DRB1 shared epitope in Caucasians with rheumatoid arthritis: a lesson learned from tic-tac-toe.

The association of rheumatoid arthritis (RA) with the major histocompatibility complex (MHC) was first reported before our current appreciation of the complexity of the MHC genetic locus. Stastny (1) reported in 1978 that the B-cell alloantigen HLA-DRw4 was found in 70% of 54 RA white patients with erosive, rheumatoid-factor-positive rheumatoid arthritis, compared to 28% of 68 normal controls (P <0.00001). Gregersen et al. (2) recognized that despite the fact that DR4 haplotypes contain at least four closely linked genes, only HLA-DRB1 exhibited a difference between the various subtypes of DR4, which was restricted to the codons surrounding amino acid position 70 of the molecule. The stretch of amino acids from amino acid residues 70-74, which corresponds to the third hypervariable region, has since been referred to as the HLA-DRB1 shared epitope (SE) (Figure 1). The HLA-DRB1 alleles with this motif that are most common in Caucasian populations are: *0401; *0404; *0405; *0408; *0101; *0102; and *1001 (3). Weyand et al. (4) reported that among RA patients with chronic, rheumatoid factor positive erosive RA, the presence of two SE-containing alleles was associated with nodular disease and joint surgery.
Hemorheological Disorders in Diabetes Mellitus

The objective of the present study is to review hemorheological disorders in diabetes mellitus. Several key hemorheological parameters, such as whole blood viscosity, erythrocyte deformability, and aggregation, are examined in the context of elevated blood glucose level in diabetes. The erythrocyte deformability is reduced, whereas its aggregation increases, both of which make whole blood more viscous compared to healthy individuals. The present paper explains how the increased blood viscosity adversely affects the microcirculation in diabetes, leading to microangiopathy.
Introductory Mathematics of the Analytic Hierarchy Process 1. Observations on the Limits to Human Capacity to Process Information

1. Observations on the Limits to Human Capacity to Process Information General limitations on human performance are very familiar in the literature of psychology [1,4] and are often classed together as cognitive spans. Such limits are widely known as “memory span”, “attention span”, apprehension span”, “perceptual span”, “span of absolute judgment”, “central computing space” and “channel capacity”. They are limits on the number of sensations, impressions or distinctions that can be held in mind briefly and grasped at once, or used as a basis for making judgments. Absolute judgment is the identification of the magnitude of some simple stimulus –for instance, the brightness of a light, the loudness of a tone, or the curvature of a linein terms of standards in memory about similar stimuli. This is in contrast with relative judgment that is the identification of some relation between two stimuli both present to the observer. In experiments on absolute judgment, an observer is considered to be a communication channel. There is an amount of information in the stimuli, and there is another amount of information in the observer’s responses. The overlap is the stimulus-response correlation as measured by the amount of transmitted information. One increases the amount of information received and then measures the amount transmitted after processing. If the observer’s absolute judgments are sufficiently “accurate” or consistent, the response would transmit most of the input information. However with increased information there is also the likelihood of more errors. The goal then is to test the limits of the observer’s absolute judgments when the information is increased. One would also expect that when the amount of input information is increased, the transmitted information would increase at first and then level off at some value. This value is the channel capacity of the observer. Channel capacity is the greatest amount of information about the stimulus that can be transmitted as an absolute judgment by the observer. It is an upper limit on the observer’s ability to respond to the stimuli received.
Nerve Growth Factor Increases in Pancreatic β Cells After Streptozotocin-Induced Damage in Rats

We investigated short-term in vivo and in vitro effects of streptozotocin (STZ) on pancreatic β cells. Male Wistar rats were treated with 75 mg/kg STZ, and, after 4 hrs blood glucose and insulin were measured and islet cells were isolated, cultured for 16 hrs, and challenged with 5.6 and 15.6 mM glucose. Treated rats showed hyperglycemia (14 mM) and a 70% decrease in serum insulin levels as compared with controls. Although insulin secretion by isolated β cells from STZ-treated rats was reduced by more than 80%, in both glucose concentrations, nerve growth factor (NGF) secretion by the same cells increased 10-fold. Moreover, NGF messenger RNA (mRNA) expression increased by 30% as compared with controls. Similar results were obtained in an in vitro model of islet cells, in which cells were exposed directly to STZ for 1, 2, and 4 hrs and then challenged for 3 hrs with the same glucose concentrations. Our data strongly suggest that an early increase in NGF production and secretion by β cells could be an endogenous protective response to maintain cell survival and that diabetes mellitus may occur when this mechanism is surpassed.
[Effect of epidermal growth factor receptor on airway remodeling in asthmatic mice and its mechanism].

OBJECTIVE To explore the relationship of airway remodeling with epidermal growth factor receptor (EGFR) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) levels in asthmatic mice and the effect of EGFR tyrosine kinase inhibitor (AG1478) on airway remodeling. METHODS Twenty-four male BALB/c mice were randomly divided into three groups: normal control, asthma, AG1478-treated. Mice were sensitized and challenged with ovalbumin (OVA) and a mouse mode1 of asthma was prepared. Collagen deposition was determined in Masson-stained lung sections. Periodic acid Schiff (PAS) staining was used to observe the proliferation of goblet cells. Immunohistochemistry was used to determine the protein expression of HB-EGF. RT-PCR was used to determine the mRNA expression of HB-EGF and EGFR. RESULTS The characteristic changes of airway remodeling occurred in the asthma group. The expression of HB-EGF and EGFR in the epithelial cells of bronchi in the asthma group was significantly higher than that in the normal control group. Compared with the asthma group, the AG1478-treated group had decreased inflammation reactions, decreased collagen deposition and proliferation of goblet cells and lower expression of EGFR and HB-EGF. CONCLUSIONS EGFR tyrosine kinase inhibitor (AG1478) ameliorates the progression of airway remodeling in mice with asthma by inhibitions of EGFR and HB-EGF expression and EGFR signal pathway.
Un modèle de génération de dilemmes de prohibition et d'obligation en environnement virtuel

Dans le cadre du projet Maccoy Critical, nous souhaitons former les apprenants a la gestion des situations critiques telles que les dilemmes. Un dilemme correspond a une situation ou il n’existe pas de bonne solution, c’est-a-dire a une situation qui mene a des consequences negatives dans tous les cas. Notre objectif est d’utiliser des modeles de connaissances pour en extraire les proprietes necessaires a l’emergence de dilemmes. Notre approche consiste pour cela a developper un systeme de scenarisation capable de generer des dilemmes sans avoir a les ecrire a l’avance. Dans cet article nous presentons cette approche et exposons une preuve de concept appliquee a la conduite automobile.
Pour le plaisir des femmes : boire et grignoter pendant le travail obstétrical, rêve ou réalité ?

Le jeune au cours du travail est une question en suspens. En pesant, le pour et le contre, les arguments suivants ont ete exposes selon des etudes et des avis: l'incidence du syndrome de Mendelson est rare, sa mortalite et sa morbidite maternelles sont graves, la physiologie de la grossesse et d'autres facteurs tel l'obesite favorisent des complications anesthesiques, les effets du jeune sont plutot nefastes, les besoins caloriques peuvent etre apportes par les boissons isotoniques, et les parturientes se sentent en securite de pouvoir boire et grignoter. Nous avons realise une enquete sur les attitudes et les perceptions des sages-femmes et des anesthesistes a l'egard du jeune. 52 questionnaires ont ete remplis par les sages-femmes et 9 par les anesthesistes. L'etude revele, tout d'abord, une forte heterogeneite dans les pratiques et dans le raisonnement du jeune. Ce dernier se revele parfois en contradiction avec la pratique du professionnel. Ainsi, 2/3 des anesthesistes autorisent la prise orale et 2/3 des sages-femmes accedent au souhait de la patiente, cependant 5 anesthesistes ont des raisons de pratiquer le jeune. La connaissance des modalites de la prise orale, la coordination des sages-femmes et des anesthesistes et, l'information aux patientes, paraissent insuffisantes. Nous avons donc formule une proposition de procedure pour ameliorer le confort des parturientes. Celle-ci inclut : de permettre a boire tout au long du travail et dans le postpartum immediat et, de grignoter dans la premiere phase du travail, et ce a partir d'une selection de patiente a bas risques; de prevoir la pose de catheter de peridurale dans des situations a risques; d'informer la patiente au cours de la consultation d'anesthesie.
SOCIJALNO POŽELJNO ODGOVARANJE I STRUKTURA LIČNOSTI – DIMENZIONALNA I TIPOLOŠKA PERSPEKTIVA

Prvi cilj ovog rada jeste da ispita odnos socijalno poželjnog odgovaranja (SPO) sa ARC tipologijom (Asendorpf-Robins-Caspi), koja polazi od pretpostavke o tri tipa licnosti: rezilijentnom, hiperkontrolisanom i hipokontrolisanom i testira hipotezu o povisenim skorovima na SPO kod tipa rezilijentnih. Drugi cilj ovog rada je da ispita relacije SPO sa sedam bazicnih dimenzija licnosti. U istraživanju je ucestvovalo 1322 ispitanika (51.7% ženskog pola), starosti od 18 do 77 godina (AS = 33.63). Primenjena je IPIP simulacija skale Malo verovatnih vrlina (Mvv), kao upitnicka operacionalizacija SPO, i skracena verzija upitnika Velikih Pet plus dva, koji operacionalizuje sedam leksickih dimenzija licnosti u srpskom jeziku. Analiza latentnih klasa je sprovedena u zajednickom prostoru dimenzija VP+2 i Mvv. Analizom su identifikovana tri tipa licnosti: neadaptirani, rezilijentni i suzdržani. Rezultati idu u prilog pretpostavkama o relacijama SPO i tipova licnosti, pri cemu tip rezilijentnih postiže umereno povisene skorove na SPO, tip suzdržanih prosecne skorove, dok tip neadaptiranih postiže umereno snižene skorove na SPO. Kako bi se ispitale relacije SPO sa dimenzijama licnosti primenjena je univarijatna analiza kovarijanse, uz kontrolu pola, starosti i obrazovanja. Rezultati potvđuju nalaze prethodnih istraživanja o relacijama SPO sa dimenzijama licnosti iz perspektive Petofaktorskog modela, ali takođe ukazuju na veliki znacaj evaluativnih dimenzija za razumevanje SPO, koje su uz Savesnost i Ekstraverziju najbolji prediktori SPO.
Julgamento ético-ideológico dos estudantes de contabilidade de São Paulo

Este trabalho buscou estudar a etica profissional no âmbito da contabilidade, sob o ponto de vista dos estudantes, visto que muito embora seja uma tematica relevante para se identificar o comportamento etico dos futuros profissionais da area, ainda e pouco explorado no Brasil. Se por um lado a pratica de atitudes eticas por parte dos contadores seja algo esperado, por outro lado, a importância do estudo formal da etica nos cursos de graduacao ainda e pouco pesquisado se for comparado com outras areas da profissao contabil. Nesse cenario, entendese que seja relevante para a classe contabil, e para a sociedade no geral, estudar-se o julgamento etico dos futuros contadores durante o processo de sua formacao. Para cumprir com os objetivos desta pesquisa descritiva, foi utilizada uma abordagem quantitativa, com questionarios contendo perguntas fechadas, nos quais se buscava observar tanto o perfil quanto o julgamento etico dos estudantes de contabilidade. Abrangeu uma amostra composta por 348 alunos de 6 cursos de graduacao da grande Sao Paulo. Para a analise dos dados foram utilizadas tecnicas estatisticas, tais como estatistica descritiva, regressoes multiplas e analise multivariada de variância, que permitiu o cruzamento das diversas informacoes coletadas. Um dos resultados identificou que o fator sexo e determinante no perfil etico dos estudantes, em que as mulheres apresentaram um maior rigor etico, enquanto que os homens alem de apresentaram-se menos rigorosos, mostram-se mais dispostos a cometerem acoes antieticas quando os beneficios destas acoes refletirem ganhos financeiros imediatos. Os perfis idealistas e relativistas identificados na pesquisa, tambem apresentaram influencia significativa nos julgamentos eticos dos alunos, mediante situacoes de dilema profissional. Por fim, e talvez o resultado mais importante, e por que nao dizer mais surpreendente, refere-se a media geral do julgamentos pessoal dos participantes da pesquisa, que demonstra uma tendencia a uma atitude mais rigorosamente etica na rotina do exercicio da profissao. Objetivamente, entende-se que os resultados deste estudo contribuem para que as IES revejam o conteudo e a metodologia adotados na disciplina de Etica Geral e Profissional do Contabilista.
First Description of Onychomycosis Caused by Chrysosporium keratinophilum in Captive Bennett's Wallabies (Macropus rufogriseus rufogriseus)

Abstract Seven Bennett's wallabies (Macropus rufogriseus rufogriseus) presented within a period of several months with onychodystrophy, onychomadesis, and severe digital tumefaction. Histopathologic findings included a pseudocarcinomatous hyperplasia of the claw matrix surrounding a cavity filled with keratin and septate hyphae stained with periodic acid Schiff reagent. The fungal species Chrysosporium keratinophilum was identified on cultures. The wallabies were orally treated with ketoconazole (15 mg/kg s.i.d.) for 20 wk. Material and enclosures were cleaned and sprayed with 0.2% enilconazole solution once a month over a period of 4 mo. No improvement of advanced cases was observed, but no new case appeared for the next 6 mo. The positive mycological culture and the invasion of tissues on histopathologic examination suggested that the fungal species C. keratinophilum was implicated in this claw disease. This is the first report of onychomycosis caused by C. keratinophilum in animals.
Interaction of Plasminogen Activator Inhibitor Type-1 (PAI-1) with Vitronectin (Vn): Mapping the Binding Sites on PAI-1 and Vn

Abstract The serpin plasminogen activator inhibitor type-1 (PAI-1), as the primary physiological inhibitor of both urokinasetype (uPA) and tissuetype (tPA) plasminogen activator, plays an important role in the regulation of the fibrinolytic system as well as in extracellular remodeling in both physiological and pathophysiological processes. In plasma as well as in the extracellular matrix PAI-1 binds to vitronectin (Vn), an interaction that affects the function of both proteins. As PAI-1/Vn interaction has a significant regulatory function in fibrinolysis, thrombolysis, and cell adhesion in cancer spread, there is a strong interest in defining the binding sites on PAI-1 and Vn as the basis of a rational design of novel drugs that may modulate PAI 1/Vnmediated effects. In this minireview, we give an overview on the approaches to define the Vn binding site of PAI-1 and vice versa. Although in the case of PAI-1 the region around αhelix E and αhelix F of PAI 1 has been demonstrated to be important for its interaction with Vn, the precise location of the Vnbinding region has not completely been resolved. The major highaffinity PAI-1 binding region of Vn is localized within the Nterminal somatomedin B (SMB) domain of Vn. There are indications for at least one other lowaffinity PAI-1 binding site in the Cterminal region of Vn, which seems to be involved in the formation of larger PAI-1/Vn complexes.
Visualization methods for sustainable planning

In urban planning, both measuring and communicating sustainability are among the most recent concerns. Therefore, the primary emphasis of this thesis concerns establishing metrics and visualization techniques in order to deal with indicators of sustainability. First, this thesis provides a novel approach for measuring and monitoring two indicators of sustainability - urban sprawl and carbon footprints – at the urban neighborhood scale. By designating different sectors of relevant carbon emissions as well as different household categories, this thesis provides detailed information about carbon emissions in order to estimate impacts of daily consumption decisions and travel behavior by household type. Regarding urban sprawl, a novel gridcell-based indicator model is established, based on different dimensions of urban sprawl. Second, this thesis presents a three-step-based visualization method, addressing predefined requirements for geovisualizations and visualizing those indicator results, introduced above. This surface-visualization combines advantages from both common GIS representation and three-dimensional representation techniques within the field of urban planning, and is assisted by a web-based graphical user interface which allows for accessing the results by the public. In addition, by focusing on local neighborhoods, this thesis provides an alternative approach in measuring and visualizing both indicators by utilizing a Neighborhood Relation Diagram (NRD), based on weighted Voronoi diagrams. Thus, the user is able to a) utilize original census data, b) compare direct impacts of indicator results on the neighboring cells, and c) compare both indicators of sustainability visually.