Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Zedoray Turmeric Oil for Ki67 in Precancerous Lesion of Rats Mammary

Objective:To research the treatment mechanism of Zedoray Turmeric Oil(ZTO) in precancerous lesion of rats mammary by measuring the expression of Ki67.Methods:275 SD rats were randomly divided into 7 groups:the blank control group(BC) ,the precancerous lesion model control group(PL) ,the kanglaite control group(KLT) ,the tamoxifen control group(TAM) ,the low dose group of ZTO(ZTO-L) ,the middle dose group of ZTO(ZTO-M) and the high dose group of ZTO(ZTO-H) . Animal model of precancerous lesion of rats mammary was induced bye DMBA and was treated by ZTO,KLT and TAM for 4 weeks.All the animals were killed from 8th to 14th week once every two weeks.Examination of histopathology was performed and measured the expression of Ki67 by immunohistochemistry.Results:The positive rate of expression of Ki67 and the positive-cell rate trend to increase in every groups except BC corresponding to normal,usual ductal hyperplasia(UDH) ,atypical ductal hyperplasia(ADH) and invasive carcinoma.In ADH,the positive rate of expression of Ki67 of ZTO,KLT and TAM was much lower than PL(P 0.05) . The positive-cell rate of Ki67 of ZTO,KLT and TAM was much lower than PL(P 0.05) ,and the positive-cell rate of Ki67 of ZTO was much lower than KLT and TAM(P 0.05) . In the case of ADH which the expression of Ki67 was positive,the positive-cell rate of Ki67 of ZTO-H was was much lower than ZTO-L(P 0.05) . Conclusion:Zedoray Turmeric Oil decrease the proliferation of breast epithelial tissue in precancerous lesion of rats mammary induced by DMBA,which could be the mechanism to prevent the breast cancer.
Historical record of Corallium rubrum and its changing carbon sequestration capacity: A meta-analysis from the North Western Mediterranean

Background There is a scarcity of long time-span and geographically wide research on the health status of Corallium rubrum, including limited research on its historical ecology and carbon sequestration capacity. Objectives To reconstruct the temporal trends of the most reported C. rubrum population parameters in the Northwestern Mediterranean Sea and to determine the changes in total carbon sequestration by this species. Data sources Quantitative and qualitative, academic and grey documents were collected from scientific web browsers, scientific libraries, and requests to scientists. Study eligibility criteria Documents with original information of basal diameter, height and/or weight per colony, with a depth limit of 60 m in the Catalan and Ligurian Seas were analyzed. Synthesis methods We calculated yearly average values of C. rubrum biometric parameters, as well as estimated total weight, carbon flux, and carbon fixation in the structures of C. rubrum’s colonies. Results In both study areas, the values of the selected morphometric parameters for C. rubrum decreased until the 1990s, then increased from the 2000s, with average values surpassing the levels of the 1960s (Ligurian Sea) or reaching levels slightly lower than those of the 1980s (Catalan Sea). The difference in carbon sequestered between the oldest (1960s: Ligurian Sea; 1970s: Catalan Sea) and the lowest (1990s) biomass value of colonies is nearly double. Limitations Quantitative data previous to the 1990s are very limited. Information on recent recovery trends in C. rubrum parameters is concentrated in a few areas and biased towards colonies in marine protected areas, with scarce quantitative information from colonies in other areas. Conclusions The halt in the C. rubrum decreasing trend coincided with the exhaustion of tree-like colonies and the first recovery response due to effective protection measures in some areas. Nevertheless, C. rubrum climate change mitigation capacity through carbon sequestration can be drastically reduced from its potential in only a few decades.
Assessment of the executive functions of moderate preterm children in preschool age

Abstract This study assesses EF and socioemotional development in 30 MPT children between 4 and 6 years, comparing them with 31 FT children. Working Memory was assessed with Digit Span and Corsi Block, verbal inhibitory control and cognitive flexibility with The Shape School Test, visuomotor inhibition with Go/No-Go and socioemotional development with SDQ for parents. In our study, MPT preschoolers had a poorer working memory, inhibitory control and verbal cognitive flexibility, and more emotional problems compared. Our results suggest that there is no safe gestational age in prematurity, for this reason, EF of preterm children should be evaluated at an early age, so early intervention plans can be implemented, preventing preterm from entering primary education in disadvantage.
Evaluation of a Novel Audit Tool for Medication Reconciliation at Hospital Discharge.

Background Discharge medication reconciliation (MedRec) is designed to reduce medication errors and inform patients and key postdischarge providers, but it has been difficult to implement routinely in Canadian hospitals. Objectives To evaluate and optimize a new discharge MedRec quality audit tool and to use it at 3 urban teaching hospitals. Methods The discharge MedRec quality audit tool, developed by the Canadian Patient Safety Institute and the Institute for Safe Medication Practices Canada, was assessed and modified to improve comprehensiveness, clarity, and quality. The modified tool was then used to evaluate the quality of the discharge MedRec process for adult patients discharged to home from the general internal medicine service at 3 academic hospitals. Postdischarge telephone interviews were conducted with consenting patients, their community pharmacists, and their family doctors. Results The audit tool required modification to include aspects of admission MedRec, high-risk medication discrepancies, and direct communication of discharge MedRec to key follow-up providers. Thirty-five patients (mean age 67.7 years, standard deviation [SD] 18.0 years; 17 [49%] women), with a mean of 8.8 (SD 4.5) prescribed medications at discharge, participated in the discharge MedRec evaluation. Documentation of any discharge MedRec was found for only 1 patient (3%), and no discharge MedRec was carried out by pharmacists. Postdischarge follow-up interviews elicited major gaps in communication with community pharmacists and with family physicians, which could lead to serious medication errors. Conclusions The modified audit tool was useful for identifying gaps in the quality of discharge MedRec.
Meta-analysis of the prognostic significance of FOXC2 in various tumors

Objective Many studies have focused on correlations between forkhead box protein C2 (FOXC2) and various tumors but discrepant results have been reported. Thus, we conducted this meta-analysis to assess the prognostic role of FOXC2 in tumors. Methods Four electronic databases (PubMed, Embase, Web of Science, and SinoMed) were screened through September 2019. Results The final analysis included 15 reports and 2115 patients; results suggested that cancer patients with FOXC2 had worse overall survival (hazard ratio 2.14, 95% confidence interval (CI) 1.74–2.64), cancer-specific survival (hazard ratio 2.65, 95% CI 1.44–4.89), and disease-free survival (hazard ratio 1.93, 95% CI 1.49–2.50) than patients lacking FOXC2. Conclusions The presence of FOXC2 was associated with poor survival in cancer patients. FOXC2 could be a promising prognostic marker in the future.
Informed choice in modern contraceptive method use: pattern and predictors among young women in India

Abstract Research on informed choice in modern contraceptive method acceptance by young married women is pertinent in the broader context of individual freedom and reproductive rights, especially in countries where women continue to have limited control over their reproductive and contraceptive choices. This study in India asked: (1) is young married women’s acceptance of specific modern contraceptive methods an informed choice? and (2) what are the enablers and barriers to informed choice? The study used data for currently married women aged 15–24 (N = 20,752) from the fourth round of the National Family Health Survey (NFHS-4) conducted in 2015–16. A Method Information Index (MII) was calculated as a measure of informed choice from the percentage of users who responded ‘yes’ to all three questions on: whether they were informed about methods other than the one they received, told about the method-specific side-effects, and advised what to do if they experienced side-effects. Binary logistic regression analysis was carried out to examine the adjusted effect of factors associated with the MII separately for female sterilization, the intrauterine device and the oral contraceptive pill, and a combined MII including all three methods plus injectables. One-fifth of the study women used any modern contraceptive method at the time of survey, of which only 36% had fully informed choice. The likelihood of being informed about the methods was significantly higher among those using the oral contraceptive pill (OR: 1.75, CI 1.58–1.94), IUD (OR: 2.23, CI 1.97–2.52) and injectables (OR: 1.37, CI 0.97–1.94) compared with those who were sterilized. Informed choice varied by region and the socioeconomic profile of the users. Inadequately informed choice violates the reproductive rights of young women and might result in higher post-use health problems, discontinuation of and unmet need for contraceptives, unintended pregnancies, induced abortions and regret, adversely affecting women’s health. Training of health/family planning workers in India about the importance of reproductive rights is urgently required to enhance informed contraceptive choice and improve the health of young married women.
Validation of the Latin American Spanish version of the face-name associative memory exam in a Colombian Sample

Abstract Objective The Face-Name Associative Memory Exam (FNAME) has been used to detect subtle cognitive changes in clinically normal older adults at increased risk for Alzheimer’s disease. FNAME assesses learning and delayed recall for face-name pairs. The aim of this study is to introduce a Latin American Spanish version of the FNAME (LAS-FNAME), examine its psychometric properties, and provide preliminary normative data in a sample of clinically normal, Spanish-speaking individuals from Antioquia, Colombia. Method 59 clinically-normal individuals (71% females) were recruited by the Grupo de Neurociencias in Antioquia (Colombia). Age ranged from 27 to 82 years (M = 50.31, SD = 15.32) and years of education ranged from 2 to 17 years (M = 9.02, SD = 4.11). All participants completed the LAS-FNAME and a brief neuropsychological evaluation. We examined associations between age, education, and sex and performance on the LAS-FNAME. Internal consistency, convergent and discriminant validity were also assessed. Test-restest reliability was computed for a subset of participants (n = 32). Results LAS-FNAME exhibited moderate convergent validity with other memory measures (Free and Cued Selective Reminding Scale, r s=.465, p<.01; Wechsler Memory Scale III - Logical Memory Delayed Recall, r s=.479, p<.01). The subscales of the LAS-FNAME exhibited adequate internal consistency (α=.825). Test-retest reliability analyses demonstrated consistency of scores over time. Normative data was stratified by age (<50, 50–65, >65) and low and high educational attainment (≤8 and >8 years of education, respectively). Conclusions The LAS-FNAME is a valid and reliable measure to assess memory in clinically normal, Spanish-speaking individuals from Colombia for clinical and research purposes.
Age, Sex, and Repeated Measures Effects on NASA's "Cognition" Test Battery in STEM Educated Adults.

BACKGROUND: Cognition is a neurocognitive test battery created at the University of Pennsylvania and adapted by the National Aeronautics and Space Administration (NASA). It comprises 10 neurocognitive tests that examine multiple domains, and has been validated in a normative sample of STEM-educated adults and compared to NASA's WinSCAT battery.METHODS: The purpose of this study was to follow the original sample to assess Cognition and WinSCAT's test-retest reliability and age, sex, and test-retest interval effects on performance.RESULTS: Performance on both Cognition and WinSCAT decreased with age but improved with repeated administration due to practice effects, and men had higher scores than women on tasks that required vigilant attention, spatial reasoning, and risk-taking behaviors. Assessment of test-retest reliability showed intraclass coefficients for efficiency ranging from 0.417 to 0.810, reflecting the broad nature of constructs assessed by Cognition.DISCUSSION: Results largely matched predictions, with some counter-intuitive results for test-retest reliability interval.Lee G, Moore TM, Basner M, Nasrini J, Roalf DR, Ruparel K, Port AM, Dinges DF, Gur RC. Age, sex, and repeated measures effects on NASA's "Cognition" Test Battery in STEM educated adults. Aerosp Med Hum Perform. 2020; 91(1):18-25.
Association of Genetic Variation With Keratoconus.

Importance Keratoconus is a condition in which the cornea progressively thins and protrudes in a conical shape, severely affecting refraction and vision. It is a major indication for corneal transplant. To discover new genetic loci associated with keratoconus and better understand the causative mechanism of this disease, we performed a genome-wide association study on patients with keratoconus. Objective To identify genetic susceptibility regions for keratoconus in the human genome. Design, Setting, and Participants This study was conducted with data from eye clinics in Australia, the United States, and Northern Ireland. The discovery cohort of individuals with keratoconus and control participants from Australia was genotyped using the Illumina HumanCoreExome single-nucleotide polymorphism array. After quality control and data cleaning, genotypes were imputed against the 1000 Genomes Project reference panel (phase III; version 5), and association analyses were completed using PLINK version 1.90. Single-nucleotide polymorphisms with P < 1.00 × 10-6 were assessed for replication in 3 additional cohorts. Control participants were drawn from the cohorts of the Blue Mountains Eye Study and a previous study of glaucoma. Replication cohorts were from a previous keratoconus genome-wide association study data set from the United States, a cohort of affected and control participants from Australia and Northern Ireland, and a case-control cohort from Victoria, Australia. Data were collected from January 2006 to March 2019. Main Outcomes and Measures Associations between keratoconus and 6 252 612 genetic variants were estimated using logistic regression after adjusting for ancestry using the first 3 principal components. Results The discovery cohort included 522 affected individuals and 655 control participants, while the replication cohorts included 818 affected individuals (222 from the United States, 331 from Australia and Northern Ireland, and 265 from Victoria, Australia) and 3858 control participants (2927 from the United States, 229 from Australia and Northern Ireland, and 702 from Victoria, Australia). Two novel loci reached genome-wide significance (defined as P < 5.00 × 10-8), with a P value of 7.46 × 10-9 at rs61876744 in patatin-like phospholipase domain-containing 2 gene (PNPLA2) on chromosome 11 and a P value of 6.35 × 10-12 at rs138380, 2.2 kb upstream of casein kinase I isoform epsilon gene (CSNK1E) on chromosome 22. One additional locus was identified with a P value less than 1.00 × 10-6 in mastermind-like transcriptional coactivator 2 (MAML2) on chromosome 11 (P = 3.91 × 10-7). The novel locus in PNPLA2 reached genome-wide significance in an analysis of all 4 cohorts (P = 2.45 × 10-8). Conclusions and Relevance In this relatively large keratoconus genome-wide association study, we identified a genome-wide significant locus for keratoconus in the region of PNPLA2 on chromosome 11.
Islamic fasting leads to an increased abundance of Akkermansia muciniphila and Bacteroides fragilis group: A preliminary study on intermittent fasting.

BACKGROUND/AIMS It has been largely accepted that dietary habits affect intestinal microbiota composition. In this pilot study, we hypothesized that time-restricted feeding, which can be regarded as a type of intermittent fasting, may have a distinct effect on intestinal microbiota. Ramadan fasting is an excellent model to understand how time-restricted feeding affect the microbiota. MATERIALS AND METHODS A total of nine subjects were included in this study during Ramadan, consisting of 17 h of fasting/day during a 29-day period. Stool samples were collected at baseline and the day of the end of Ramadan. 16S rRNA qPCR assay has been performed for quantification of Akkermansia muciniphila, Faecalibacterium prausnitzii, Bifidobacterium spp. , Lactobacillus spp. , Bacteroides fragilis group, and Enterobacteriaceae. Blood samples were also collected to test for metabolic and nutritional parameters. RESULTS A significantly increased abundance of A. muciniphila and B. fragilis group was observed in all subjects after Islamic fasting when compared with the baseline levels (p=0.004 and 0.008, respectively). Serum fasting glucose and total cholesterol levels were also significantly reduced in all of the subjects (p<0.01 and p=0.009, respectively). CONCLUSION Islamic fasting, which represents intermittent fasting, leads to an increase in A. muciniphila and B. fragilis group, which were considered as healthy gut microbiota members. Although this is a pilot study, which should be tested with larger sample size, there are a very limited number of studies in the literature on fasting and microbiota in human subjects. Thus, our present findings may contribute to the understanding of fasting-gut microbiota interaction.
The diversity and abundance of fungi and bacteria on the healthy and dandruff affected human scalp

Dandruff is a skin condition that affects the scalp of up to half the world’s population, it is characterised by an itchy, flaky scalp and is associated with colonisation of the skin by Malassezia spp. Management of this condition is typically via antifungal therapies, however the precise role of microbes in the aggravation of the condition are incompletely characterised. Here, a combination of 454 sequencing and qPCR techniques were used to compare the scalp microbiota of dandruff and non-dandruff affected Chinese subjects. Based on 454 sequencing of the scalp microbiome, the two most abundant bacterial genera found on the scalp surface were Cutibacterium (formerly Propionibacterium) and Staphylococcus, while Malassezia was the main fungal inhabitant. Quantitative PCR (qPCR) analysis of four scalp taxa (M. restricta, M. globosa, C. acnes and Staphylococcus spp.) believed to represent the bulk of the overall population was additionally carried out. Metataxonomic and qPCR analyses were performed on healthy and lesional buffer scrub samples to facilitate assessment of whether the scalp condition is associated with differential microbial communities on the sampled skin. Dandruff was associated with greater frequencies of M. restricta and Staphylococcus spp. compared with the healthy population (p<0.05). Analysis also revealed the presence of an unclassified fungal taxon that could represent a novel Malassezia species.
A high-throughput, seedling screen for plant immunity.

An understanding of how biological diversity impacts plant-microbe interactions is becoming increasingly important, particularly with respect to components of the pathogen effector arsenal and the plant immune system. While technological improvements have greatly advanced our ability to examine molecular sequences and interactions, relatively few advances have been made that facilitate high-throughput, in vivo, pathology screens. Here we present a high-throughput, microplate-based, non-destructive, seedling pathology assay, and apply it to identify Arabidopsis thaliana effector-triggered immune (ETI) responses against Pseudomonas syringae type III secreted effectors (T3SEs). The assay is carried out in a 48-well microplate format with spray inoculation, and disease symptoms are quantitatively recorded in a semi-automated manner; thereby, greatly reducing both time and costs. The assay requires only slight modifications of common labware and uses no proprietary software. We validated the assay by recapitulating known ETI responses induced by P. syringae in Arabidopsis. We also demonstrate that we can quantitatively differentiate responses from a diversity of plant genotypes grown in the same microplate. Finally, we show that the results obtained from our assay can be used to perform genome-wide association studies (GWAS) to identify host immunity genes, recapitulating results that have been independently obtained with mature plants.
A future of automated image contouring with machine learning in radiation therapy

Automated image contouring is showing improvements in efficiency for a number of clinical tasks in radiotherapy. While atlas segmentation has proven moderately beneficial, the next generation of algorithms based on convolutional neural networks is already pointing to improvements in precision and efficiency. This work provides a broad overview of the benefits of machine learning when applied to these tasks.
Republished: DPP-4 inhibitor (sitagliptin)-induced seronegative rheumatoid arthritis

In conjunction with BMJ Case Reports , DTB will feature occasional drug-related cases that are likely to be of interest to readers. These will include cases that involve recently marketed drugs for which there is limited knowledge of adverse effects and cases that highlight unusual reactions to drugs that have been marketed for several years. Sitagliptin is a dipeptidyl peptidase-4 inhibitor commonly used in the treatment of type 2 diabetes mellitus for glycaemic control. Concerns have arisen regarding adverse events caused by this drug, particularly concerning arthralgias. Here, we report on a 56-year-old man being treated with sitagliptin who developed inflammatory arthritis after taking the drug for 6 months. The patient presented with pain, swelling and erythema in multiple joints and was eventually diagnosed with seronegative rheumatoid arthritis (RA) under the 2010 American College of Rheumatology/European League Against Rheumatism classification criteria. His symptoms continued for several months after stopping sitagliptin and eventually went into remission after a tapered course of steroids, hydroxychloroquine and methotrexate. Furthermore, the patient is HLA-DRB3 positive, a genetic marker that is still being investigated for its role in the pathogenesis of RA and that may have been a predisposing factor in the development of this patient’s inflammatory arthropathy. Like most diabetes medications, sitagliptin can disturb inflammatory and immune pathways.1 Although most therapies for diabetes target and inhibit the inflammatory system, there have been multiple studies, as well as a Food and Drug Administration (FDA)-issued warning attributing the development of arthralgia to the use of sitagliptin.1–7 In particular, the activity of dipeptidyl peptidase (DPP)-4 is thought to be inversely related to rheumatoid arthritis (RA), and hindering DPP-4 may contribute to RA development.3 6 Furthermore, the link between certain human leucocyte antigens (HLAs) and adverse drug reactions, such as arthralgias, is an ongoing area of research. Here, …