Miracle-dz/UniFAR-FTUs huggingface dataset:
Chromatin Opening and Potentiation of Transcriptional Activation by the General Regulatory Factors , ABF 1 and RAP 1 , in the Budding Yeast Saccharomyces cerevisiae

ABF1 and RAP1 are abundant, multifunctional, site specific DNA binding proteins found in the budding yeast Saccharomyces cerevisiae (Miyake et al. 2002, Morse 2000, Shore 1994) . RAP1 and ABF1 are essential for cell viability and have roles in gene activation and gene silencing, telomere structure and recombination; their multifunctional character has caused them to be termed General Regulatory Factors, or GRFs. Binding sites for ABF1 and RAP1 are found in a large number of promoters, and have been shown to be important for activation of many of the corresponding genes. Genomewide localization, or “ChIP-on-chip” experiments indicate that RAP1 and ABF1 bind to about 300 promoters each, and RAP1 has been found to bind to 122 of 137 ribosomal protein genes (Lee et al. 2002, Lieb et al. 2001). Genes containing RAP1 or ABF1 sites principally comprise ribosomal protein genes and genes encoding proteins involved in amino acid biosynthesis, regulation of carbon source, and sporulation. Many of these genes are among those most highly expressed in the yeast genome. Chromatin Opening and Potentiation of Transcriptional Activation by the General Regulatory Factors, ABF1 and RAP1, in the Budding Yeast Saccharomyces cerevisiae ARUNADEVI YARRAGUDI and RANDALL H MORSE Wadsworth Center, NY State Department of Health and SUNY at Albany School of Public Health, Department of Biomedical of Sciences, Albany, NY 12201 (USA)
Predictive Direct Torque Control of Matrix Converter fed Permanent Magnet Synchronous Machines

A predictive Direct Torque Control method for Matrix Converters fed Permanent Magnet Synchronous Machines drives is proposed in this paper. The voltage vector from the Matrix Converter which ensures minimum torque ripple is selected based on a predictive algorithm. A look-up table for Direct Torque Control using Matrix Converters has been developed which delivers a set of three vectors, at every sample period, that fulfil the torque and flux demands. The predictive algorithm computes the torque error that would be present applying each vector and selects the one that contributes with the minimum error. Simulation results, which confirm the good performance of the proposed predictive Direct Torque Control using Matrix Converters are shown.
Multisensory speech perception of young children with profound hearing loss.

The contribution of a two-channel vibrotactile aid (Trill VTA 2/3, AVR Communications LTD) to the audiovisual perception of speech was evaluated in four young children with profound hearing loss using words and speech pattern contrasts. An intensive, hierarchical, and systematic training program was provided. The results show that the addition of the tactile (T) modality to the auditory and visual (A+V) modalities enhanced speech perception performance significantly on all tests. Specifically, at the end of the training sessions, the tactile supplementation increased word recognition scores in a 44-word, closed-set task by 12 percentage points; detection of consonant in final position by 50 percentage points; detection of sibilant in final position by 30 percentage points; and detection of voicing in final position by 25 percentage points. Significant learning over time was evident for all test materials, in all modalities. As expected, fastest learning (i.e., smallest time constants) was found for the AVT condition. The results of this study provide further evidence that sensory information provided by the tactile modality can enhance speech perception in young children.
Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin*

The activity regenerating luciferin from the luminescent product oxyluciferin was found in the protein fraction of a lantern extract from Photinus pyralis. The protein, luciferin-regenerating enzyme (LRE), was purified to homogeneity by ammonium sulfate precipitation followed by successive column chromatography on Ultrogel AcA34, S-Sepharose FF, Q-Sepharose FF, TSKgel super Q 5pw and TSKgel G3000 SWXL. This enzyme was a single polypeptide with a molecular mass of 38 kDa. LRE converted oxyluciferin to 2-cyano-6-hydroxybenzothiazole and thioglycolic acid. In the presence of d-cysteine, 2-cyano-6-hydroxybenzothiazole was turned over into luciferin. The same activities were detected in the extracts from two Japanese fireflies,Luciola cruciata and Luciola lateralis. We have cloned a cDNA encoding LRE from poly(A)+ RNA of the lantern ofP. pyralis using reverse transcription-polymerase chain reaction, 5′-RACE (rapid amplification of cDNA ends) and 3′-RACE. The primary structure of LRE from P. pyralisdeduced from the nucleotide sequence was shown to consist of 308 amino acids with a molecular weight of 33,619. The cDNA was successfully expressed under the control of the tac promoter inEscherichia coli.
A 9 mW Highly-Digitized 802.15.4 Receiver Using Bandpass $\Sigma\Delta$  ADC and IF Level Detection

A low power (9 mW) highly-digitized 2.4 GHz receiver for sensor network applications (IEEE 802.15.4 LR-WPAN) is realized by a 0.18 mum CMOS process. We adopted a novel receiver architecture adding an intermediate frequency (IF) level detection scheme to a low-power complex fifth-order continuous-time (CT) bandpass SigmaDelta modulator in order to digitalize the receiver. By the continuous-time bandpass architecture, the proposed SigmaDelta modulator requires no additional anti-aliasing filter in front of the modulator. Using the IF detector, the achieved dynamic range (DR) of the overall system is 95 dB at a sampling rate of 64 MHz. This modulator has a bandwidth of 2 MHz centered at 2 MHz. The power consumption of this receiver is 9.0 mW with a 1.8 V power supply.
Antimalarial dihydroisocoumarins produced by Geotrichum sp., an endophytic fungus of Crassocephalum crepidioides.

Three novel dihydroisocoumarin derivatives (1-3) with antimalarial, antituberculous, and antifungal activities have been isolated by bioassay-guided fractionation from an endophytic fungus, Geotrichum sp. , collected from Crassocephalum crepidioides. Structures were established as 7-butyl-6,8-dihydroxy-3(R)-pent-11-enylisochroman-1-one (1), 7-but-15-enyl-6,8-dihydroxy-3(R)-pent-11-enylisochroman-1-one (2), and 7-butyl-6,8-dihydroxy-3(R)-pentylisochroman-1-one (3) using spectroscopic data.
Canine Thoracolumbar Intervertebral Disk Disease : Pathophysiology , Neurologic Examination , and Emergency Medical Therapy *

Thoracolumbar intervertebral disk disease (IVDD) is a broad term, encompassing disk degeneration and clinical neurologic disease due to disk herniation. Canine IVDD has been reported to be the reason for presentation in 23 of every 1000 cases seen in 13 veterinary colleges in the United States and Canada, and it is the most common cause of thoracolumbar myelopathy with paraspinal hyperesthesia. A thorough, integrated understanding of spinal anatomy, pathophysiology, and neurologic function forms the foundation for medical decision-making and care for dogs with clinical signs of intervertebral disk herniation.
Impact of Recurrent and Chronic Pain on Child and Family Daily Functioning: A Critical Review of the Literature

&NA; The author reviewed the current status of research on the impact of recurrent and chronic pain on everyday functioning of children and families and organized the research findings around the specific life contexts (e.g., school, peers) that may be affected by pain. Although findings demonstrate that many different aspects of child and family life are affected by pain, the prevalence and severity of children's functional limitations associated with pain remain unknown. Few treatment studies for pediatric recurrent and chronic pain have focused on enhancing children's functioning. It has been shown, however, that functional outcomes can be improved by cognitive‐behavioral interventions. Recommendations for research on functional outcomes and implications for clinical practice are discussed.
Invitation à l’ivresse

Cet article propose de decrypter une vignette ethnographique produite lors d’un terrain de recherche base en France. En passant du temps avec des personnes hebergees dans un centre d’hebergement et de reinsertion sociale specialise dans l’accueil d’usagers sans abri et dependants aux drogues, le chercheur s’est retrouve face a un dilemme : accepter ou non de boire de l’alcool ? Cette epreuve de positionnement permettra d’aﬃner la comprehension du terrain et d’envisager sous un angle diﬀerent la question de la ritualite de consommations importantes d’alcool.
Local e-government Benchlearning: Impact analysis and applicability to smart cities benchmarking

We claim that local e-services benchmarking studies summarized in indexes do little to enhance city managers’ and academics’ understanding of actual e-government performance, or to improve the e-services offered by cities. We undertook a different benchmarking approach, focused on learning best practices among cities, in late 2008 and early 2009. A benchlearning methodology (BLM) was developed, and a pilot study with 15 European cities was carried out. In this paper, we present the actual impact of the benchmarking study with respect to improvements in services, as the effectiveness of e-government benchmarking has rarely been evaluated. We discuss and analyse the results of a survey carried out in the same 15 cities four years after the pilot study. This paper presents evidence that BLM helped cities to identify good practices that they could learn from, and that some e-services were subsequently improved. The survey reveals that some changes are needed in the benchmarking methodology. The main one is the updating of the BLM bottom-up e-services catalogue, which is deeply discussed within the changing context of Smart Cities, especially the enlargement of the ecosystem of e-services to include citizens, the third sector, entrepreneurs, companies and other actors. A second one is the measurement of the adoption of e-services by citizens, also rarely assessed.
Détection de messages abusifs au moyen de réseaux conversationnels

Le nombre et la taille des communautes en ligne ne cessent de s'accroitre depuis l'ap-parition du Web. Cependant, la moderation du contenu produit par leurs utilisateurs est toujours essentiellement realisee manuellement. L'automatisation de cette tâche permettrait de reduire son cout financier, mais la majorite des approches utilisees en production s'appuient unique-ment sur le contenu des messages et apparaissent tres sensibles aux methodes d'obfuscation intentionnelles. Dans cet article, nous proposons une methode pour l'extraction de reseaux conversationnels a partir des logs de conversations (chat), et nous etudions le pouvoir discri-minant d'un ensemble de mesures topologiques pour un probleme de classification consistant a identifier les messages abusifs. Notre approche atteint un niveau de performance inattendu, comparable a celui obtenu precedemment sur les memes donnees avec une approche unique-ment basee sur le contenu.
Antigenic analysis of the major outer membrane protein of Chlamydia trachomatis with murine monoclonal antibodies

We prepared monoclonal antibodies against prototype strains of the 15 serovars of Chlamydia trachomatis and identified a subset of reagents that reacted with the major outer membrane protein(s) (MOMPs) of one or more serovars. We then determined the specificities of these anti-MOMP monoclonal antibodies by radioimmunoassay and immunoblot assays against the 15 serovars of C. trachomatis and a C. psittaci strain. We identified 14 different anti-MOMP antibody specificities, including serovar-, several orders of subspecies-, and species-specific determinants. In addition, one antibody reacted with all C. trachomatis serovars and a C. psittaci strain, indicating the presence of a genus-specific epitope on MOMP. Many of the cross-reactions of the subspecies-specific antibodies were similar to those previously reported by use of the microimmunofluorescence technique. We also observed a number of cross-reactions that were unexpected but consistent with data derived by the microimmunofluorescence test. All antibodies, except the genus-specific antibodies, reacted with whole elementary bodies in a radioimmunoassay, suggesting surface exposure of the epitopes. These data confirm and extend previous observations that MOMPs among C. trachomatis serovars are antigenically complex and diverse. In addition, these data indicate that the cross-reaction patterns of some monoclonal antibodies directed against MOMP are similar to those detected by the microimmunofluorescence test and are consistent with the hypothesis that such determinants are contained within MOMPs.
Fiscal Federalism and Foreign Transfers: Does Inter-Jurisdictional Competition Increase Foreign Aid Effectiveness?

This paper empirically studies the impact of decentralization and inter-jurisdictional competition on foreign aid effectiveness. For this purpose we examine a commonly used empirical growth model, considering different measures of fiscal decentralization. Our panel estimations reveal that expenditure decentralization and inter-jurisdictional competition - reflected by the degree of tax revenue decentralization - negatively impact aid effectiveness. We therefore conclude that donor countries should carefully consider how both anti-poverty instruments - foreign assistance and decentralization - work together.
On secondary representations of the second

The emergence of microwave and optical frequency standards with accuracies close to those of caesium primary frequency standards has led to the concept of secondary representations of the second. The recently-formed Joint Working Group of the CCL / CCTF has now recommended as secondary representations to the CCTF / CIPM one microwave radiation in <sup>87</sup>Rb and three optical radiations in <sup>199</sup>Hg<sup>+</sup>, <sup>88</sup>Sr<sup>+</sup> and <sup>171</sup>Yb<sup>+</sup>. This paper describes the rationale, evolution and development of criteria for acceptance of a radiation as a secondary representation, together with a review of the JWG assessment and recommendation of the four radiations already proposed.
Universal arc representation using EMTP

A preliminary description of a new arc representation has been introduced in . In which, the Modified Mayr (P-/spl tau/ model) arc equations are solved in the transient analysis control system (TACS) field of the electromagnetic transient program (EMTP) and represented in the power network by TACS controlled voltage source. In this paper, substantial improvements of this representation are carried out. A performance comparison with the EMTP built-in Avdonin model is incorporated to validate the proposed representation effectiveness. SF6 breakers with experimentally defined arc parameters are used to perform this comparison. The thermal limiting curve is computed by the proposed and the built-in models and compared with the measured characteristic. Also, universality of representation is verified via carrying out different arc model examples of circuit breaker and transmission-line arcing faults. The study results validated the proposed representation regarding accuracy and universality.
Practice Software Maintenance-an Industrial Experience

This paper gives an overview of the software maintenance process of Hitachi Software Engineering (HSK) Co., Ltd in Japarr-iocludmg its success and failure cases. It discusses HSK's software processes, issues, use of specific tools and techniques such as IMOZU diagrams, and approaches in solving problems. These include the requirement capturing process, reverse-engineering techniques, environmental changes, change management, and project management. By discussing actual project experiences, we point to certain lessons and their implications for research in software maintenance. We also present the latest re-engineering CASE tools, such as RE-ENGINEERING and SEWB3, used at HSK.
Phosphorylation sites in ligand-induced and ligand-independent activation of the progesterone receptor.

Steroid hormone receptors are phosphoproteins that undergo hyperphosphorylation upon binding of hormone. The mechanism and the role of this reaction remain poorly understood. Two-dimensional analysis of ligand-free progesterone receptor (PR) tryptic digests showed the existence of seven main phosphopeptides. Incubation of the cells with the progestin R5020 led to a global increase in the levels of PR phosphorylation. However, the same phosphopeptides were seen, and their levels of labeling relative to each other were unchanged. A similar result was observed after incubation of cells with the antiprogestin RU486. The antiprogestin ZK98299 demonstrated only half of the activity of RU486 in terms of receptor hyperphosphorylation, but the same phosphopeptides, proportionally labeled to the same extent, were observed by chromatography electrophoresis. Ligand-induced DNA binding did not play a role in receptor hyperphosphorylation since the mutant delta 547-592, which is devoid of the first zinc finger region, exhibited the same phosphopeptides, labeled to the same extent, as did wild-type receptor after incubation of cells with hormone. These results suggest that the same kinase(s) act in vivo on ligand-free and on agonist or antagonist-bound progesterone receptor. Binding of different ligands produces different conformational changes in the ligand binding domain of the receptor which enhance, to varying extents, affinity of the receptor for the kinase(s). The DNA binding region also plays a role in the interaction with the kinase(s), although binding to DNA per se is not necessary for the hyperphosphorylation of the receptor to take place. (ABSTRACT TRUNCATED AT 250 WORDS)
Screening for the Early Detection of Ovarian Cancer

Evaluation of: Menon U, Gentry-Maharaj A, Hallett R et al.: Sensitivity and specificity of multimodal and ultrasound screening for ovarian cancer, and stage distribution of detected cancers: results of the prevalence screen of the UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS). Lancet Oncol. 10, 327–340 (2009). A randomized, controlled trial was undertaken to assess the use of serum CA125 and ovarian ultrasound as diagnostic markers in the early detection of ovarian cancer. The trial consisted of 202,638 postmenopausal women who were assessed annually between 2001 and 2005. A nonscreen group and two screening groups were investigated. The screening groups included serum CA125, which was based on a rate-of-change algorithm, plus transvaginal ultrasound (in at-risk women); and an ovarian ultrasound group only, prior to clinical review and surgery. The sensitivity, specificity and positive predictive values for all primary ovarian and tubal cancers for the combined test group was 89.4, 99.8 and 43.3%, respectively, and for the ultrasound group 84.9, 98.2 and 5.3%, respectively, with a significant difference in specificity between groups. The combined test provides the requisite specificity for a screening test for ovarian cancer in postmenopausal women.
Enumeration by frame group and range groups

Wider extension is here given to the theory developed in an earlier paper, in which the enumeration of certain configurations or combinatorial entities was made to depend on two or more groups (range groups). The present paper recognizes for the first time the essential part played by an additional group, the frame group, which includes all the range groups as subgroups, and which was implicit in the earlier theory as the symmetric group of the degree of the range groups. The use of frame groups which are not symmetric greatly extends the range of application of the theory, and allows its development in terms of abstract groups. By the use of the conjugate sets of the subgroups (both cyclic and noncyclic) of the frame group, instead of merely the conjugate sets of its operations, a complete solution is obtained for the problem (solved only for special cases in the earlier paper) of determining the invariance groups admitted by the various configurations enumerated.
Expression of Connective Tissue Growth Factor and Bone Morphogenetic Protein-7 in Pseudomonas aeruginosa-induced Chronic Obstructive Pulmonary Disease in Rats

Abstract Repeated intratracheal injection of Pseudomonas aeruginosa (PA) in male Wistar rats was used to investigate the role of chronic infection in the development of chronic obstructive pulmonary disease (COPD) and the possible involvement of connective tissue growth factor (CTGF) and bone morphogenetic protein-7 (BMP-7) in this process. Injections of PA or normal saline solution were given for 8 weeks and the rats observed for a further 8 weeks. In addition to arterial blood gas, lung function and lung pathology measurement during this time period, protein and mRNA expression of CTGF and BMP-7 were measured, and the correlation of expression of CTGF and BMP-7 with pathological changes in the lung was evaluated. Repeated intratracheal PA infection in rats caused reduction in body weight, hypoxia, carbon dioxide retention, compromised lung function, chronic inflammation, thickening of the tracheal and arterial walls, and emphysema, changes consistent with those of COPD. Rats with PA infection also had increased CTGF and decreased BMP-7 expression, suggesting that both CTGF and BMP-7 are involved in the occurrence and development of airway remodeling. Our findings suggest that repeated airway infection is not only a factor resulting in deterioration of COPD, but is also a risk factor for its development, and that CTGF and BMP-7 are involved in the pathogenesis of this condition.
An intrinsic treatment of ubiquity phenomena in higher-order computability models ( Part I , draft version )

We introduce some simple conditions on typed partial combinatory algebras (viewed as models of higher-order computability) which suffice for an axiomatic development of some non-trivial computability theory, including various forms of the Kreisel-LacombeShoenfield theorem, along with the author’s earlier ‘ubiquity’ theorems which say that a wide range of computability models all give rise to the same ‘hereditarily total’ functionals. This results in a cleaner, more ‘intrinsic’ formulation of the latter theorems, which we are moreover able to generalize to cover certain computability models of a non-deterministic character. Within our axiomatic framework, we also investigate extensions of the ubiquity results to a much wider class of types, arising from simple types via subset and quotient constructions. This enables us to cover many types of interest that arise naturally in mathematical practice (e.g. in real or complex analysis), and establishes the existence of robust computability notions at such types. In the present Part I, we introduce our axioms and the conceptual framework, and carry through the required proofs for firstand second-order types. The focus here is on the Kreisel-Lacombe-Shoenfield theorem, which we adapt to our axiomatic setting in various ways. In Part II, we will extend our results to type 3 and above, using the construction of Normann which we exploited in our earlier work on ubiquity.