Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular Detection and Therapeutic Management of Exudative Epidermitis in Swine

Of 343 swine examined, 34 (9.91%) were found to be affected with exudative epidermitis from which 39 S. hyicus and 6 S. aureus isolates were recovered. Out of 39 S. hyicus, 34 S. hyicus isolates were found to be positive for virulence genes of which, 6 isolates (17.64%) were positive for exhA gene and 28 isolates (82.35%) were positive for exhD gene with an exhibited band size of 316 bp and 588 bp respectively in PCR. Early recovery of swine with exudative epidermitis was found with amoxyclav with supportive therapy than ceftriaxone and tazobactam combination with supportive therapy though the recovery rates with both the antibiotics were 100 per cent.
Pregnancy-associated Changes in the Hemostatic System

Pregnancy, from implantation to parturition, presents unique and profound challenges to a women's hemostatic system. During pregnancy, potentially catastrophic bleeding can occur during implantation and endovascular trophoblast invasion of the maternal spiral arteries. The risk of hemorrhage reaches a peak during the third stage of labor when the placenta is shorn from the decidua basalis exposing 120 spiral arteries largely denuded of their smooth muscle, and thus, their ability to constrict in response to injury. These challenges are met by dramatic changes in the local uterine, and systemic hemostatic systems. The net effect of these changes is to increase the efficiency of clotting and to impair fibrinolysis. Unfortunately, they also lead to an increase in the prevalence of venous thromboembolism, which is otherwise uncommon in reproductive age women.
Mobility independent and dependent predictive services management in wireless/mobile multimedia network

This paper focuses on the management of services independent and dependent from mobility in adaptive multimedia networking, where the bandwidth of an ongoing multimedia flow can be dynamically adjusted. Two classes of service have been considered: MIP and MDP classes, defined in integrated services for mobile wireless environments. In order to offer an adaptive QoS (soft QoS), increasing the total wireless system utilization, a rate adaptation algorithm has been considered. The algorithm is based on a user utility function and its target is to maximize this function. The host mobility can impact on the number of handoffs and on channel states (fading), so the rate adaptation accounts for the degradation of channel state conditions. An admission control algorithm has been considered too in order to regulate the access. The admission control can use the pre-reservation phase among potentially visited cells from mobile hosts for MIP services and considers only the bandwidth availability on current cells for MDP services. The performance evaluation of the system has been evaluated in terms of total bandwidth utilization, average user utility perceived by mobile users, and system outage probability.
The Relationship between Five-Factor Model and DSM-5 Personality Traits on Patients with Borderline Personality Disorder

Results: The results indicate that there is a positive significant relationship between neuroticism (N) with emotional lability (r=0.34, P<0.01), anxiousness (r=0.286, P<0.01) and impulsivity (r=0.229, P<0.05). Also, there is a significant relationship between openness (O) and emotional lability (r=0.316, P<0.01) and risk taking (r=0.193, P<0.05), and negative relation with impulsivity (r=-0.244, P<0.01), separation insecurity(r=0.194, P<0.05), and depressivity (r=0.19, P<0.05). In addition, results showed that there is positive significant relationship between FFM and DSM-5 personality traits with DSM-IV-TR BPD symptoms (P< 0.01). Conclusion: Results were in line with findings from previous studies and were explained in part by considering how facets/traits of the FFM and DSM-5 personality traits map onto the concept of BPD. Also, the present study helps understand the adequacy of dimensional approach to evaluate personality pathology, specifically on Iranian sample. A B S T R A C T Article info:
A comparison of image restoration approaches applied to three‐dimensional confocal and wide‐field fluorescence microscopy

We have compared different image restoration approaches for fluorescence microscopy. The most widely used algorithms were classified with a Bayesian theory according to the assumed noise model and the type of regularization imposed. We considered both Gaussian and Poisson models for the noise in combination with Tikhonov regularization, entropy regularization, Good's roughness and without regularization (maximum likelihood estimation). Simulations of fluorescence confocal imaging were used to examine the different noise models and regularization approaches using the mean squared error criterion. The assumption of a Gaussian noise model yielded only slightly higher errors than the Poisson model. Good's roughness was the best choice for the regularization. Furthermore, we compared simulated confocal and wide‐field data. In general, restored confocal data are superior to restored wide‐field data, but given sufficient higher signal level for the wide‐field data the restoration result may rival confocal data in quality. Finally, a visual comparison of experimental confocal and wide‐field data is presented.
Anti–Tumor Necrosis Factor-&agr; Treatment Improves Endothelial Function in Patients With Rheumatoid Arthritis

Background—Rheumatoid arthritis (RA) is associated with accelerated atherosclerosis and increased cardiovascular morbidity and mortality. Striking similarities exist in the inflammatory and immunologic response in RA and atherosclerosis. Indeed, adhesion molecules and cytokines, tumor necrosis factor (TNF)-&agr; in particular, are key mediators of joint inflammation and of vascular dysfunction and progression of atherosclerotic vascular disease. Hence, the aim of the present study was to assess the effect of chronic antiinflammatory treatment with the anti-TNF-&agr; antibody infliximab on disease activity and endothelial function in patients with active RA. Methods and Results—Eleven RA patients (mean age 46±5 years; disease duration 9±2 years) with high disease activity despite treatment with stable doses of methotrexate (≤25 mg/wk) and prednisone (≤10 mg/d) were investigated. Clinical status and endothelium-dependent and -independent vasodilation of the brachial artery as assessed by high-resolution ultrasound were measured before and after 12 weeks of infliximab therapy. Flow-mediated vasodilation improved from 3.2±0.4% to 4.1±0.5% (P =0.018), whereas endothelium-independent vasodilation with nitroglycerin and baseline diameter remained unchanged (13.6±1.2% versus 12.8±1.4%, P =0.98, and 3.74±0.15 versus 3.66±0.11 mm, P =0.54, respectively). Disease activity score (DAS28) was significantly reduced, from 5.6±0.3 to 3.5±0.6 (P =0.002). Erythrocyte sedimentation rate and C-reactive protein were lowered from 34±7 to 19±5 mm/h (P =0.04) and from 38±11 to 15±10 mg/L (P =0.08), respectively. Conclusions—This is the first study to show that anti-TNF-&agr; treatment improves endothelial function in RA. The data suggest that in RA, endothelial dysfunction is part of the disease process and is mediated by TNF-&agr;.
SUPEROXIDE DISMUTASE OF MAMMALIAN NERVOUS SYSTEM

Superoxide dismutase was assayed in portions of the nervous system (a) by inhibition of tetrazolium reduction by oxygen radicals, generated enzymically, and (b) by inhibition of tetrazolium reduction by oxygen radicals generated by oxidation of NADH in presence of phenazine methosulphate. Superoxide dismutase activity was found in beef brain, retina and adrenal medulla, as well as in brain, retina and lungs of adult and of newborn rats. Preliminary experiments with rats exposed to hyperbaric oxygen showed no alteration of enzyme activities in tissues of newborn and adult animals. The possible role of superoxide dismutase in the nervous system is discussed.
Ebola Virus Selectively Inhibits Responses to Interferons, but Not to Interleukin-1β, in Endothelial Cells

ABSTRACT Ebola virus infection is highly lethal and leads to severe immunosuppression. In this study, we demonstrate that infection of human umbilical vein endothelial cells (HUVECs) with Ebola virus Zaire (EZ) suppressed basal expression of the major histocompatibility complex class I (MHC I) family of proteins and inhibited the induction of multiple genes by alpha interferon (IFN-α) and IFN-γ, including those coding for MHC I proteins, 2′-5′ oligoadenylate synthetase [2′-5′(A)N], and IFN regulatory factor 1 (IRF-1). Induction of interleukin-6 (IL-6) and ICAM-1 by IL-1β was not suppressed by infection with EZ, suggesting that the inhibition of IFN signaling is specific. Gel shift analysis demonstrated that infection with EZ blocked the induction by IFNs of nuclear proteins that bind to IFN-stimulated response elements, gamma activation sequences, and IFN regulatory factor binding site (IRF-E). In contrast, infection with EZ did not block activation of the transcription factor NF-κB by IL-1β. The events that lead to the blockage of IFN signaling may be critical for Ebola virus-induced immunosuppression and would play a role in the pathogenesis of Ebola virus infection.
Music therapy in the NICU: is there evidence to support integration for procedural support?

Optimizing physical and neurologic developmental goals are critical as many premature infants will survive throughout the neonatal intensive care period into childhood and adulthood. Receiving highly-technical and complex care in the neonatal intensive care unit (NICU) may cause harm through multiple factors that stress the immature physiology of the infant including exposing the infant to touch for assessments and invasive interventions often causing physiologic distress. Physical assessments and interventions are potential stressors that can change the stability of the infant leading to changes in vital signs1 that may require additional interventions to prevent further hypoxia and hypotension. If the hypoxia and hypotension continue, injury to the cerebral tissue may occur leading to alterations in the neurological system that may negatively affect short and long-term outcomes. Neuroprotective strategies to ameliorate the negative effects of stressors on the physiologic stability of the premature infant must be explored to prevent further consequences to these fragile infants.2 Music therapy is an emerging intervention that may help stabilize the negative physiologic changes during exposure to stressors in the NICU. Therefore, the purpose of this review was to determine what evidence exists to support the use of music therapy in the NICU during stressful events (e.g., endotracheal suctioning). This use of music therapy is different from music used on a short-term or continuous basis in the NICU, this review only examined the use of music therapy as adjunctive to procedures with preterm infants.
Effects of Music Composed by Mozart and Ligeti on Blood Pressure and Heart Rate Circadian Rhythms in Normotensive and Hypertensive Rats

There is continuing discussion on the effect of music (“Mozart effect”) on numerous functions in man and experimental animals. Radiotelemetry now allows one to monitor cardiovascular functions in freely‐moving unrestrained experimental animals. Radiotelemetry was used to monitor systolic and diastolic blood pressure (SBP, DBP), heart rate (HR), and motor activity (MA) in male normotensive WKY and hypertensive SHR animals. Rats were synchronized to a 12 h light (L): 12 h dark (D) regimen in an isolated, ventilated, light‐controlled, sound‐isolated animal container. Music (Mozart, Symphony # 40; Ligeti, String Quartet # 2) were played for 2 h at 75 dB in the animal cabin starting at the onset of L or D in a cross‐over design. Data were collected every 5 min for 24 h under control conditions and during and after music. In addition, plasma concentrations of norepinephrine (NE) were determined in unrestrained animals at 3 h intervals over 24 h. In both WKY and SHR, highly significant circadian rhythms were obtained in SBP, DBP, HR, and MA under control conditions; HR was lower and BP higher in SHR than in WKY. NE was circadian rhythmic in both strains with higher values in D; the increase in NE with immobilization was much more pronounced in SHR than in WKY. The music of Mozart had no effect on either parameter in WKY, neither in L nor in D. In contrast, in SHR, the music of Mozart presented in L significantly decreased HR and left BP unaffected, leading to a small decrease in cardiac output. The music of Ligeti significantly increased BP both in L and in D and reflexively reduced HR in L, the effects being long‐lasting over 24 h. Interestingly, white noise at 75 dB had no effect at all on either function in both strains. The effects of both Mozart and Ligeti cannot be attributed to a stress reaction, as stress due to cage switch increased HR and BP both in WKY and SHR. The study clearly demonstrates that music of different character (tempo, rhythm, pitch, tonality) can modify cardiovascular functions in freely‐moving rats, with SHR being more sensitive than normotensive animals. The relative contribution of the characteristics of the two pieces of music, however, needs further evaluation.
Improving Color Text Sharpness in Images with Reduced Chromatic Bandwidth

Current digital imaging systems often reduce the chromatic bandwidth to speed transmission or save storage. While this has become routine for most video applications, it is also becoming true of digital camera systems. Chromatic bandwidth is usually reduced by converting the RGB image into a single luminance and two color difference images, and then subsampling the color difference images. The motivation lies in the reduced bandwidths of the visual system for the color signals, as evidenced by contrast sensitivity function (CSF) data. Systems using chromatic subsampling can exhibit visual equivalence to otherwise identical non-subsampled systems, despite the physical color blur introduced by subsampling. While this equivalence holds true for images of real scenes and optically captured scenes, problems arise with synthetically generated imagery, such as color geometrics and type fonts. The possible sources of these problems include: 1) deviation from natural image chromatic power spectra, 2) nonlinear masking effects between the luminance and chrominance visual mechanisms, and 3) filtering processes in the luminance-chrominance domain create signals outside of the RGB gamut. By taking advantage of nonlinear perceptual effects and the gamut clipping issues, we have developed a simple point-processing algorithm that accomplishes a reduction in the visibility of chromatic blur.
DNA repair on the brain

Xeroderma pigmentosum (XP), Cockayne syndrome (CS), and trichothiodystrophy (TTD) constitute a family of sun-sensitive human diseases (1). They are caused by mutations in the components of the nucleotide excision repair (NER) system and in the postreplication repair system involving the low-fidelity polymerase H (also known as XP variant or XPV). XP was initially recognized as a disease involving a genetic predisposition to extremely high levels of skin carcinogenesis from solar exposure. The relationship between DNA damage from solar UVB exposure and deficiencies in repair, leading to mutagenesis, genetic instability, and eventual carcinogenesis, provides a deceptively simple explanation for the major clinical features of XP and has provided a paradigm for environmentally induced human cancer. These diseases are, however, much more complex at all levels (1, 2), as the new report in this issue by Murai et al. (3) indicates (Fig. 1). NER has two main pathways: GGR and TCR. Global repair involves the genes XPC and XPE and is generally slower and modulated by p53 (4), which also modulates the pol H pathway (5, 6). TCR results in a more rapid repair of the transcribed strand of expressed genes, and involves the two CS genes (CSA, CSB), several of the XP genes (especially XPA, -B, and -D), and the mismatch repair system (1). Some of the genes are involved in both pathways, especially XPA. The XPG endonuclease is also a cofactor for a glycosylase (endonuclease III, encoded by nth) that acts on oxidative damage (7). Mutations in XPB and XPD also can give rise to diseases that combine one or more of the three main disorders, XP, CS, and TTD, depending in part on the precise site of the mutations in the genes.
Influence of Lactobacillus spp. from an Inoculant and of Weissella and Leuconostoc spp. from Forage Crops on Silage Fermentation

ABSTRACT Lactobacillus spp. from an inoculant andWeissella and Leuconostoc spp. from forage crops were characterized, and their influence on silage fermentation was studied. Forty-two lactic acid-producing cocci were obtained from forage crops and grasses. All isolates were gram-positive, catalase-negative cocci that produced gas from glucose, and produced more than 90% of their lactate in the d-isomer form. These isolates were divided into groups A and B by sugar fermentation patterns. Two representative strains from the two groups, FG 5 and FG 13, were assigned to the species Weissella paramesenteroides and Leuconostoc pseudomesenteroides, respectively, on the basis of DNA-DNA relatedness. Strains FG 5, FG 13, and SL 1 (Lactobacillus casei), isolated from a commercial inoculant, were used as additives to alfalfa and Italian ryegrass silage preparations. Lactic acid bacterium counts were higher in all additive-treated silages than in the control silage at an early stage of ensiling. During silage fermentation, inoculation with SL 1 more effectively inhibited the growth of aerobic bacteria and clostridia than inoculation with strain FG 5 or FG 13. SL 1-treated silages stored well. However, the control and FG 5- and FG 13-treated silages had a significantly (P < 0.05) higher pH and butyric acid and ammonia nitrogen contents and significantly (P < 0.05) lower lactate content than SL 1-treated silage. Compared with the control silage, SL 1 treatments reduced the proportion ofd-(−)-lactic acid, gas production, and dry matter loss in two kinds of silage, but the FG 5 and FG 13 treatments gave similar values in alfalfa silages and higher values (P < 0.05) in Italian ryegrass silage. The results confirmed that heterofermentative strains of W. paramesenteroides FG 5 andL. pseudomesenteroides FG 13 did not improve silage quality and may cause some fermentation loss.
Implant design and its effects on osseointegration over time within cortical and trabecular bone.

Healing chambers present at the interface between implant and bone have become a target for improving osseointegration. The objective of the present study was to compare osseointegration of several implant healing chamber configurations at early time points and regions of interest within bone using an in vivo animal femur model. Six implants, each with a different healing chamber configuration, were surgically implanted into each femur of six skeletally mature beagle dogs (n = 12 implants per dog, total n = 72). The implants were harvested at 3 and 5 weeks post-implantation, non-decalcified processed to slides, and underwent histomorphometry with measurement of bone-to-implant contact (BIC) and bone area fraction occupied (BAFO) within healing chambers at both cortical and trabecular bone sites. Microscopy demonstrated predominantly woven bone at 3 weeks and initial replacement of woven bone by lamellar bone by 5 weeks. BIC and BAFO were both significantly increased by 5 weeks (p < 0.001), and significantly higher in cortical than trabecular bone (p < 0.001). The trapezoidal healing chamber design demonstrated a higher BIC than other configurations. Overall, a strong temporal and region-specific dependence of implant osseointegration in femurs was noted. Moreover, the findings suggest that a trapezoidal healing chamber configuration may facilitate the best osseointegration. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1091-1097, 2016.
Ultrahigh‐Throughput Screening to Identify E. coli Cells Expressing Functionally Active Enzymes on their Surface

We show here that E. coli bacteria that display esterases or lipases on their cell surface together with horseradish peroxidase (HRP) are capable of hydrolysing carboxylic acid esters of biotin tyramide. The tyramide radicals generated by the coupled lipase–peroxidase reaction were short‐lived and therefore became covalently attached to reactive tyrosine residues that were located in close vicinity on the surface of a bacterial cell that displayed lipase activity. Up to 120 000 biotinylated tyramide derivatives could be covalently coupled through HRP activation to the surface of a single living E. coli cell. Differences in cellular esterase activity were found to correlate with the amount of biotin tyramide deposited on the cell surface. Selective biotin tyramide labelling of cells that had lipase activity allowed their isolation by magnetic cell sorting from a 1:106 mixture of control cells. This strategy of covalently attaching a biotin label to esterase‐proficient bacteria might open new avenues to ultrahigh‐throughput screening of enzyme libraries for hydrolytic enzymes with enhanced activities or enantioselectivities.
Phenotypic and functional characterization of the hematopoietic stem cell

The purpose of this report is to demonstrate the phenotypic and functional characteristics of a primitive hematopoietic stem cell (HSC). We present evidence that an isolated murine HSC can repopulate the hematopoietic tissues of lethally irradiated recipient animals long term. By limiting dilution, as few as ten isolated stem cells can reconstitute mice for their lifetime. The stem cell which we have isolated does not copurify with colony forming unit‐spleen or radioprotect recipients from lethal radiation. The phenotypic characterization of this rare cell, which represents 0.005% of total bone marrow, includes either the absence or very low expression of markers associated with long‐term repopulating cells described by other groups. We believe this stem cell represents a very early self‐renewing stem cell in the mouse. Stem Cells 1997; 15(suppl 1): 41–45
RIDGE AUGMENTATION UTILIZING GUIDED TISSUE REGENERATION, TITANIUM SCREWS, FREEZE‐DRIED BONE, AND TRICALCIUM PHOSPHATE: CLINICAL REPORT

A mixture of demineralized freeze-dried human cortical bone and resorbable tricalcium phosphate was used in conjunction with an expanded polytetrafluoroethylene membrane to promote deposition of bone for ridge augmentation. A titanium screw was used to prevent collapse of the regenerative materials. A clinical report is presented in which an atrophic ridge was reconstructed buccolingually, permitting the placement of a root form implant in a previously untenable site. (Implant Dent 1994;3:35–37)
A Role of TCP1 in the Longitudinal Elongation of Leaves in Arabidopsis

We clarified important roles of Arabidopsis TCP1, a member of the TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) transcription factors, in the longitudinal elongation of petioles, rosette leaves and inflorescent stems. We found that the promoter of TCP1 was active in the cotyledonary petioles and the distal part of the expanding leaves, as well as the midrib region and the petiole.