Miracle-dz/UniFAR-FTUs huggingface dataset:
CCR7 mediates the migration of Foxp3+ regulatory T cells to the paracortical areas of peripheral lymph nodes through high endothelial venules

Thymus‐derived forkhead box p3+ naturally occurring regulatory T cells (nTreg) are thought to circulate throughout the body to maintain peripheral immunological self‐tolerance through interactions with dendritic cells (DCs), resulting in regulation of conventional T cells. However, the chemokine receptors, which are putatively involved in the in vivo migration of nTreg, have not been fully established. Here, we demonstrated that lymph node nTreg preferentially migrated to the paracortical area of lymph nodes after adoptive transfer, where they were observed to make contact frequently with CD8α+ DCs and CD8α− CD11b− DCs. This migration of nTreg to the paracortical areas was impaired severely when cells were prepared from CCR7‐deficient mice. However, to some extent, CCR7‐independent migration of nTreg in such CCR7‐deficient mice was also observed, but this occurred mainly in the medullary high endothelial venules. Taken together, these data provide the evidence that CCR7 mediates nTreg migration to the paracortical areas of lymph nodes under steady‐state conditions; however, CCR7‐independent migration also takes place in the medulla.
Learning to be a professional football manager: a Bourdieusian perspective

ABSTRACT This paper draws on the theoretical concepts of Pierre Bourdieu to provide an insight into aspirant football managers’ perceptions of what is required and valued at different stages of their desired managerial career journey. Drawing on interviews with candidates from one cohort of the Scottish Football Association Professional Licence (n = 19), our evidence suggests that aspirant managers have responded to changes in field logic by adopting strategies which place increased emphasis on cultural capital in the form of engagement with educational discourse. While we find evidence of instrumentality in attitudes to education, we also find evidence which emphasises the importance of habitus as an unconscious process. Educational culture is absorbed and embodied by some aspirant managers, which enlightens their actions and encourages them to adopt empowering strategies through which they seek to transform their place in the field. The paper concludes by considering potential implications for governing bodies and clubs.
The pan‐Kv7 (KCNQ) Channel Opener Retigabine Inhibits Striatal Excitability by Direct Action on Striatal Neurons In Vivo

Central Kv7 (KCNQ) channels are voltage‐dependent potassium channels composed of different combinations of four Kv7 subunits, being differently expressed in the brain. Notably, striatal dopaminergic neurotransmission is strongly suppressed by systemic administration of the pan‐Kv7 channel opener retigabine. The effect of retigabine likely involves the inhibition of the activity in mesencephalic dopaminergic neurons projecting to the striatum, but whether Kv7 channels expressed in the striatum may also play a role is not resolved. We therefore assessed the effect of intrastriatal retigabine administration on striatal neuronal excitability in the rat determined by c‐Fos immunoreactivity, a marker of neuronal activation. When retigabine was applied locally in the striatum, this resulted in a marked reduction in the number of c‐Fos‐positive neurons after a strong excitatory striatal stimulus induced by acute systemic haloperidol administration in the rat. The relative mRNA levels of Kv7 subunits in the rat striatum were found to be Kv7.2 = Kv7.3 = Kv7.5 > >Kv7.4. These data suggest that intrastriatal Kv7 channels play a direct role in regulating striatal excitability in vivo.
Some Connections Between Classical Coding and Network Coding Over Erroneous Cyclic Networks

Recently, a framework was given for linear error-correcting network codes (LENCs) over cyclic networks on commutative rings. When the alphabet is considered as a rational power series ring, an LENC is referred to as a convolutional error-correcting network code (CENC). Recently, a metric was introduced for these codes based on the minimum rank distance. In this paper, a new metric is introduced for ring-based LENCs over cyclic networks based on the Hamming distance, which is referred to as the network Hamming distance. Then, some connections between maximum distance separable (MDS) LENCs and classical MDS codes are obtained. Finally, the network Hamming free distance is given for CENCs, which plays the role of the free distance for convolutional codes.
End-of-life care in the oldest old

Abstract Objective: The aim of this study was to describe the last year of life of a sample of the oldest old, focusing on care trajectories, health, social networks, and function in daily life activities. Method: Data originated from the NONA study, a longitudinal study of 193 individuals among the oldest old living in a Swedish municipality. During this longitudinal study, 109 participants died. Approximately one month after their death, a relative was asked to participate in a telephone interview concerning their relative's last year of life. One hundred two relatives agreed to participate. Results: Most of the elderly in this sample of the oldest old (74.5%) died at an institution and the relatives were mostly satisfied with the end-of-life care. The oldest old relatives estimated that the health steadily declined during the last year of life, and that there was a decline in performing of daily life activities. They also estimated that those dying in institutions had fewer social contacts than those dying in a hospital or at home. Significance of results: Care at end of life for the oldest old is challenged by problems with progressive declines in ability to perform activities of daily living and health. The findings also highlight the need to support social networks at eldercare institutions.
Cutoff Scores of the Cognitive Abilities Screening Instrument, Chinese Version in Screening of Dementia

The purpose of this study of dementia screening was to obtain different cutoff scores of the Cognitive Abilities Screening Instrument, Chinese versions (CASI C-2.0) for subjects with different educational backgrounds. The diagnosis of dementia was based on the Diagnostic and Statistical Manual of Mental Disorders, ed 3 revised or ed 4 criteria. To diagnose Alzheimer’s disease, the guidelines of the National Institute of Neurological and Communicative Disorders and Stroke and Alzheimer’s Disease and Related Disorders Association was followed. The severity of dementia was determined on the Clinical Dementia Rating scale. Altogether 2,096 subjects, aged 65 years and more, were included. Of them, 1,178 were normal and 918 were demented. Their performance on CASI C-2.0 was influenced by their education and age. Gender difference on CASI C-2.0 scores was only significant in the illiterate, but not in the literate group. We recommend that the population be divided into three levels, namely those who (1) had no formal education (Edu = 0); (2) received 1–5 years of schooling (Edu = 1–5), and (3) received 6 or more years of education (Edu ≧6). The cutoff scores of CASI C-2.0 in the diagnosis of dementia in these three educational groups were as follows: Edu = 0: 49/50 (sensitivity = 0.83; specificity = 0.85); Edu = 1–5: 67/68 (sensitivity = 0.83; specificity = 0.91), and Edu ≧6: 79/80 (sensitivity = 0.89; specificity = 0.90).
Sickle cell–haemoglobin E (HbSE) compound heterozygosity: a clinical and haematological study

The paucity of clinical reports in the world literature suggests that, as a disease entity, haemoglobin SE compound heterozygosity is of negligible importance. In view of the significant community prevalence of this haemoglobinopathy in the Sultanate of Oman where it is the second most prevalent sickling disorder, a hospital study of 12 SE compound heterozygotes from six unrelated Arab families was undertaken to determine their clinico‐haematological features. Our findings were compared with those reviewed in the literature. Clinical and haematological evaluation was carried out by conventional methods including chromatographic haemoglobin analysis. At least 50% of those studied were asymptomatic throughout the study period but sickling‐related complications occurred in the rest and included the acute chest syndrome (1/12), severe vaso‐occlusive skeletal pain (2/12), frontal bossing (1/12) possibly indicative of significant chronic haemolysis and recurrent infections of the urinary tract (1/12). Steady‐state haemoglobin levels fell within the reference range while MCV and MCH values were, as expected, reduced in most cases; nevertheless, concomitant inheritance of α‐thalassaemia trait was also likely. Red cell morphology was striking by the absence or rarity of pseudo‐sickled cells in the blood films of many patients during the steady state and in crises. Bearing in mind the prevalence of 0.05% of SE compound heterozygosity in Oman, the findings in this single study of the largest number of SE patients and their relatives confirm the predominantly asymptomatic nature of this sickling disorder in individuals in the community at large. HbF levels do not appear to explain the heterogeneous nature of this haemoglobinopathy. Correlation of the variable clinical and haematological features of SE cases with their α‐globin gene status and β‐cluster haplotypes (linked to the βs‐ and βe‐genes) merits a separate investigation, which is being currently organized.
QT Dispersion in Renal Transplant Recipients

An increased QT dispersion (QTd) is associated with a variety of cardiac diseases and predicts sudden death. Although chronic renal failure patients and patients on hemodialysis are shown to have an increased QTd, evidence of increased QTd in renal transplant patients is scarce. In this study, renal transplant patients were evaluated to find out if they had an increased QTd. Thirty-four renal transplant recipients aged 35 ± 8 years and 34 healthy control subjects aged 34 ± 8 years were included in the study. The mean time after transplantation was 51.8 ± 40.4 (range 5–154) months. The QT interval was measured by 12-lead electrocardiogram, and the QTd was defined as the difference between the maximum and minimum QT interval. Bazett’s formula was used to correct for the heart rate (QTc). Both QTd and QTc dispersion (QTcd) in renal transplant patients were compared with those of control subjects. All patients underwent transthoracic echocardiographic assessment and 24-hour ambulatory blood pressure monitoring. Renal transplant recipients had similar QTd (37 ± 15 vs. 39 ± 17 ms) and QTcd (50 ± 18 vs. 55 ± 20 ms) compared to control subjects. QTd and QTcd were similar in patients with and without left ventricular hypertrophy (QTd 37 ± 14 vs. 36 ± 17 ms and QTcd 50 ± 14 vs. 49 ± 21 ms, respectively). No association was found between QTd and left ventricular mass index or blood pressure measurements. The QTd was not found to be increased in renal transplant recipients as compared with that of healthy controls in this study. Normalization of the QTd after renal transplantation may be through the correction of several factors responsible for increased QTd in uremic patients.
Viruses in sewage waters during and after a poliomyelitis outbreak and subsequent nationwide oral poliovirus vaccination campaign in Finland

During an outbreak of paralytic poliomyelitis in Finland in 1984 and 1985 the widespread circulation of the causative wild-type serotype 3 poliovirus in the population was documented by demonstrating the virus in sewage water specimens in 13 different locations in the greater Helsinki district and in 13 other cities or towns all over the country. After the nationwide campaign with oral poliovirus vaccine in 1985, poliovirus serotypes 2 and 3 were readily isolated from sewage waters for up to 2 months, whereas type 1 poliovirus seemed to disappear from the sewage more rapidly. All of these isolates were temperature sensitive and therefore most likely vaccine related. The efficacy of the vaccination campaign in regard to elimination of the epidemic type 3 strain was evaluated by a follow-up study on viruses in sewage waters continued for 12 months through the subsequent expected season of poliomyelitis. Several types of enteroviruses, including five vaccine-related poliovirus strains, were identified in the 72 virus-positive specimens out of 93 studied. No wild-type polioviruses were found, indicating the success of the campaign.
The regeneration of electroreceptors in kryptopterus

The regeneration of ampullary electroreceptors was studied in the living catfish, Kryptopterus, by differential interference contrast optics. Electroreceptors in this transparent catfish are found, among other places, along the proximal portion of each anal fin ray, while the distal portion does not contain electroreceptors. Upon interruption of the sensory innervation, the electroreceptors disappear but regenerate when the skin is reinnervated. In this study, we tested the role of the skin and nerve in receptor regeneration with the following two experiments. First, a plug of fin containing electroreceptors was removed to determine whether electroreceptors could form in regenerated skin after the complete removal of all of the receptors within an interradial zone of the anal fin. Second, a portion of anal fin that contained electroreceptors was excised and a graft of electro receptor‐free (EF) fin was sutured in its place to determine whether epidermis that does not normally contain these receptors can be induced to form them. These grafts were compared to control grafts taken from proximal electroreceptor‐containing (EC) fin. By 2 weeks following surgery, receptors were found in regenerated fin tissue and within the EC grafts. Electroreceptors also formed within most of the EF grafts. As electroreceptor regeneration does not require the presence of degenerated organs, and as electroreceptors can form in fin that normally does hot contain receptors, we suggest that the formation of electroreceptors does not require old target sites and that epidermal cells can be induced to form receptors upon contact by regenerating axons. We discuss as well the factors that influence the pattern of receptor reappearance.
Mild Traumatic Brain Injury (Concussion) During Combat: Lack of Association of Blast Mechanism With Persistent Postconcussive Symptoms

ObjectiveTo determine whether screening for a blast mechanism of concussion identifies individuals at higher risk of persistent postconcussive symptoms (PCS). SettingUnited States Army post. Participants3952 US Army infantry soldiers were administered anonymous surveys 3 to 6 months after returning from a yearlong deployment to Iraq. Main Outcome MeasuresSelf-reported concussion (defined as an injury that resulted in being “dazed, confused, or ‘seeing stars’”; “not remembering the injury”; or “losing consciousness [knocked out]): Patient Health Questionnaire 15-item scale for physical symptoms and PCS; Posttraumatic Stress Disorder Checklist; and Patient Health Questionnaire depression module. ResultsOf the 587 soldiers (14.9% of the total sample) who met criteria for concussion, 201 (34.2%) reported loss of consciousness, and 373 (63.5%) reported only an alteration of consciousness without loss of consciousness; 424 (72.2%) reported a blast mechanism, and 150 (25.6%) reported a nonblast mechanism. Among soldiers who lost consciousness, blast mechanism was significantly associated with headaches and tinnitus 3 to 6 months postdeployment compared with a nonblast mechanism. However, among the larger group of soldiers reporting concussions without loss of consciousness, blast was not associated with adverse health outcomes. ConclusionsBlast mechanism of concussion was inconsistently associated with PCS, depending on the definition of concussion utilized. A self-reported history of blast mechanism was not associated with persistent PCS for the majority of US soldiers with concussions.
Wireless permanent magnet temperature & field distribution measurement system for IPMSMs

Permanent magnet (PM) temperature measurement/estimation in permanent magnet synchronous machines (PMSMs) is of great importance for torque control and to prevent PM demagnetization. PM temperature estimation methods can be divided into thermal models, BEMF and pulse/high-frequency signal injection based methods. All of them provide a lumped PM temperature estimation. However, the temperature distribution along the magnet is not uniform, also asymmetries among poles can exist. Analyzing the impact of the temperature variations on the magnet field distribution requires simultaneous measurement of the PM temperature and field distributions. However, this is not easy due to the large amount of temperature and field sensors required, which places significant challenges regarding the sensors integration as well as the signal acquisition and transmission. Most of temperature measurement systems using contact type sensors that have been reported use a reduced amount of sensors and relatively low sampling rates. Furthermore, they do not include field sensors. This significantly limits the analysis that can be realized. This paper presents a wireless PM temperature and field measurement system for PMSMs. The developed system provides PM temperature and field distribution maps for all the poles with high spatial resolution and bandwidth. Uses of the developed system include analysis of the surface and internal PM temperature distribution, PM field distribution, assessment of local demagnetization risks due to hot spots in PMs, as well as development and assessment of new temperature and field distribution estimation methods for PMSMs.
Correlation between Putative Inhibitory Molecules at the Dorsal Root Entry Zone and Failure of Dorsal Root Axonal Regeneration

The molecular mechanisms involved in preventing regenerating dorsal root axons from entering the spinal cord at the dorsal root entry zone (DREZ) are obscure. We used immunohistochemistry, in situ hybridization, and electron microscopy to study axonal regeneration after dorsal rhizotomy in adult rats and its relationship to cellular changes and the distribution of putative growth inhibitory molecules in this region. Astrocyte processes, ending as bulb-shaped expansions, grew up to 700 microm into the basal lamina tubes of injured roots, where regenerating axons were also present. Some of these axons approached or reached the DREZ but grew no further; others turned back toward the ganglion, suggesting the presence of repulsive cues in or near the DREZ. Tenascin-C mRNA and protein and CSPG stub immunoreactivity were strongly upregulated in the roots after rhizotomy, but were only weakly expressed in the DREZ. Tenascin-R immunoreactivity was confined to CNS tissue, and unaffected by rhizotomy. Large, rounded GFAP-negative, NG2-immunoreactive cells, a few of which were OX42 positive, were found in the DREZ following rhizotomy. Astrocyte processes projecting into the roots were tenascin-R and NG2 negative. Hence, only NG2-expressing cells and tenascin-R were appropriately situated to inhibit regeneration through the DREZ.
Implementation of Cooperation for Recycling Vehicle Routing Optimization in Two-Echelon Reverse Logistics Networks

The formation of a cooperative alliance is an effective means of approaching the vehicle routing optimization in two-echelon reverse logistics networks. Cooperative mechanisms can contribute to avoiding the inefficient assignment of resources for the recycling logistics operations and reducing long distance transportation. With regard to the relatively low performance of waste collection, this paper proposes a three-phase methodology to properly address the corresponding vehicle routing problem on two echelons. First, a bi-objective programming model is established to minimize the total cost and the number of vehicles considering semitrailers and vehicles sharing. Furthermore, the Clarke–Wright (CW) savings method and the Non-dominated Sorting Genetic Algorithm-II (NSGA-II) are combined to design a hybrid routing optimization heuristic, which is denoted CW_NSGA-II. Routes on the first and second echelons are obtained on the basis of sub-optimal solutions provided by CW algorithm. Compared to other intelligent algorithms, CW_NSGA-II reduces the complexity of the multi-objective solutions search and mostly converges to optimality. The profit generated by cooperation among retail stores and the recycling hub in the reverse logistics network is fairly and reasonably distributed to the participants by applying the Minimum Costs-Remaining Savings (MCRS) method. Finally, an empirical study in Chengdu City, China, reveals the superiority of CW_NSGA over the multi-objective particle swarm optimization and the multi objective genetic algorithms in terms of solutions quality and convergence. Meanwhile, the comparison of MCRS method with the Shapley value model, equal profit method and cost gap allocation proves that MCRS method is more conducive to the stability of the cooperative alliance. In general, the implementation of cooperation in the optimization of the reverse logistics network effectively leads to the sustainable development of urban and sub-urban areas. Through the reasonable reorganization of the entire network, recycling companies can provide more reliable services, contribute to the reduction of environmental pollution, and guarantee significant profits. Thus, this paper provides manufacturing companies, logistics operators and local governments with tools to protect the environment, while still making profits.
Analysis of New Step-up and Step-down Direct Symmetric 18-pulse Topologies for Aircraft Autotransformer-Rectifier Units

Among possible 18-pulse autotransformer-rectifier-unit (ATRU) topologies, direct symmetric circuit (DS-ATRU) demonstrates relatively low complexity, it is insensitive to impedance mismatch and distortions of input voltage and has low common mode voltage. This paper proposes new step-up and step-down direct symmetric 18-pulse ATRU topologies for aircraft application. Serious study of proposed topologies was performed. The authors analyzed the kVA ratings, effects of leakage inductance on compliance with input current harmonic requirements, power sharing between diode bridges, common mode voltage and output ripple. The comparison is carried out for the full range of the input line frequency from 400 to 800 Hz. Key analyses and results obtained with Saber simulations are presented for validation of the presented work. Some experimental results are shown
JAK-2 V617F Mutational Analysis in Primary Idiopathic Myelofibrosis: Experience from Southern Pakistan.

BACKGROUND Primary idiopathic myelofibrosis (PMF) is a clonal Ph-chromosome negative myeloproliferative neoplasm characterized by dysregulated kinase signaling and release of abnormal cytokines. In the recent past, following JAK2 V617F mutation invention, important revolution has been made in the molecular diagnostic biology of this disease. The rational of this study was to determine the mutational status of JAK2 V617F in Pakistan patients with PMF. MATERIALS AND METHODS In this cross sectional study, 20 patients with PMF were enrolled from January 2011 to December 2014. Diagnosis was based on WHO criteria for PMF. All patients were screened for G-T point mutation (V617F) in the JAK2 gene on chromosome 9 by allele specific PCR. RESULTS The mean age was 57.9 ± 16.5 years. The male to female ratio was 3:1. The frequency of JAK2 V617F positivity in our PMF patients was found to be 55%. Positive correlations of JAK2 V617F mutation were established with high TLC count, raised LDH and marked splenomegaly (P<0.05). No correlation of JAK2 V617F could be established with age and gender (P>0.05). CONCLUSIONS The JAK2 V617F mutation frequency in our PMF patients was similar to those reported previously. In our hands JAK2 V617F mutated patients expressed an aggressive disease phenotype. Screening for the mutation in all suspected PMF cases could be beneficial in differentiating patients with reactive and clonal marrow fibrosis.