Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of plant cation/H+ antiporters and how they can impact human nutrition

Characterization of Plant Cation/H Antiporters and How They Can Impact Human Nutrition. (December 2007) James Larry Morris, Jr., B.S., Texas Tech University; M.S., Texas Tech University Chair of Advisory Committee: Dr. Kendal Hirschi Calcium transporters regulate calcium fluxes within cells. Plants, like all organisms, contain channels, pumps and exchangers to carefully modulate intracellular calcium levels. Numerous Arabidopsis proteins have been characterized which can transport calcium. However, there are numerous genes whose products have not been fully characterized. One method I used to infer function was to analyze various promoter lengths of 17 putative cation transporters fused to reporter and to observe changes in the reporter expression in response to various stimuli. Using a more in depth approach I set out to characterize the function of AtCCX3. Here I show Arabidopsis AtCCX3 can suppress yeast mutants defective in vacuolar Na and K transport. AtCCX3-expressing yeast cells conferred Mn sensitivity when highly expressed. Functional epitope tagged AtCCX3 fusion proteins were localized to endomembranes in plants and yeast. Expression of AtCCX3 increased in plants treated with NaCl, KCl and MnCl2 and caused increased Na accumulation and increased K transport. Ectopic expression of AtCCX3 in tobacco produced lesions in the leaves, stunted growth, resulted in the accumulation of higher levels of numerous cations and increased protein
Cationic peptides obtained by reversible disaggregation of antibacterial proteins of bovine seminal plasma

Abstract A series of experiments was conducted to investigate the aggregating and disaggregating properties of anti-bacterial proteins. in seminal plasma. Little of the antibactenal activity of bovme seminal plasma diffused through dialysis membrane of retentivity 10 kDa at pH 7, but at pH 3 or with 0.1 mol/litre citrate at pH 7 most of the activity diffused through. Some of this activity passed at pH 3 through an ultrafilter of retentivity 2 kDa. Gel filtration chromatography of the ultrafiltrate showed some reaggregation of material in this ultrafiltrate at pH 3, and reaggregation of all of it at pH 7. A fast-migrating cationic antibacterial peptide was found by gel electrophoresis of both the acid and citrate dialysis diffusates of semmal plasma. On gel electrophoresis the same pattern of five cationic bands was found in the diffusate from acid dialysis of seminal plasma using membranes of retentivity 3.5, 8, and 12 kDa. One extra band was found with the 12 kDa membrane. All the bands were antibacteria.
The Design of Marine Remote Sensing Image Fusion Model Based on Dynamic Load Balancing Strategy

ABSTRACT Feng, X. ; Wan, M. , and Li, S. , 2020. The design of marine remote sensing image fusion model based on dynamic load balancing strategy. In: Yang, Y. ; Mi, C. ; Zhao, L. , and Lam, S. (eds.), Global Topics and New Trends in Coastal Research: Port, Coastal and Ocean Engineering. Journal of Coastal Research, Special Issue No. 103, pp. 943–946. Coconut Creek (Florida), ISSN 0749-0208. In this work, a model of marine remote sensing image fusion based on dynamic load balancing is proposed. The remote sensing image acquisition system based on Android intelligent device obtains the marine remote sensing image, and enhances and registers the image based on the fuzzy theory and template matching, respectively, to realize the image preprocessing. Based on the preprocessing results, considering the small number of objects in the marine remote sensing image, the target area is divided by the idea of quad tree structure. At the same time, the task allocation strategy of dynamic load balancing and the idea of parallel computing are used to propose the task allocation algorithm processing model of the cluster system for the fusion processing of the image in the target area, so as to achieve the fusion of marine remote sensing image. It can be known that the proposed model takes less time and has superior average gradient and clarity of image.
Spectral Irradiance Distribution in Underwater Light Field Generated by an LED Light Source

ABSTRACT Yang, P.; Wu, Z. ; Chen, Y. ; Guo, Y. ; Zhang, P., and Song, H. , 2020. Spectral irradiance distribution in underwater light field generated by an LED light source. In: Yang, Y. ; Mi, C. ; Zhao, L. , and Lam, S. (eds.), Global Topics and New Trends in Coastal Research: Port, Coastal and Ocean Engineering. Journal of Coastal Research, Special Issue No. 103, pp. 453–457. Coconut Creek (Florida), ISSN 0749-0208. Artificial underwater illumination has a wide range of applications in coastal zone research, marine engineering, marine resource exploration and development, and marine ecological environment monitoring. In this article, the distribution of spectral irradiance has been discussed for an underwater light field generated by an underwater LED light source. Theoretical representations of the spectral irradiance have been derived according to physical principles and underwater spectral irradiance measurement have been accrued out along the optical axis of the light source and in the vertical plane perpendicular to the optical axis. Experimental results show that the spectral irradiance along the optical axis of the light source decays with respect to the distance, following the law of square attenuation and exponential attenuation. The irradiance in the vertical plane approximately matches the Gaussian distribution.
Doubling the Mechanical Properties of Spider Silk by C60 Supersonic Molecular Beam Epitaxy

Spider silk is one of the most fascinating natural materials, owing to its outstanding mechanical properties. In fact, it is able to combine usually self-excluding properties, like strength and toughness that synthetic fibers fail to replicate. Here, we report a method to further enhance the already excellent mechanical properties of spider's silk, producing nanocomposite fibers where the matrix of spider silk is reinforced with C60 molecules. These are deposited by Supersonic Molecular Beam Epitaxy (SuMBE) and are able to efficiently interact with silk, as evidenced by XPS analysis. As a consequence, upon proper adjustment of the fullerene kinetic energy, the treated fibers show improved strength, Young's modulus and toughness.
Performance Evaluation Model of Economic Compensation Policy for Cultivated Land Protection in Coastal Areas Based on Propensity Value Matching Method

ABSTRACT Yang, B. , 2020. Performance evaluation model of economic compensation policy for cultivated land protection in coastal areas based on propensity value matching method. In: Yang, Y. ; Mi, C. ; Zhao, L. , and Lam, S. (eds.), Global Topics and New Trends in Coastal Research: Port, Coastal and Ocean Engineering. Journal of Coastal Research, Special Issue No. 103, pp. 19–23. Coconut Creek (Florida), ISSN 0749-0208. In order to promote the rapid economic growth of cultivated land protection in coastal areas a performance evaluation model of economic compensation policy for cultivated land protection in coastal areas was designed based on the propensity value matching method. According to the principle of remolding the economic protection value of cultivated land resources in coastal areas, the compensation model of cultivated land protection economic policy is established based on the propensity value matching method, and then the market value of cultivated land protection economy in coastal areas is calculated based on the performance evaluation elements, so as to realize the performance tendency value calculation of economic compensation policy. The empirical results show that, with the support of the propensity value matching method, the economic compensation policy of cultivated land protection in coastal areas shows an obvious trend of rapid growth within a given period of time, and the overall performance evaluation environment in the region is gradually improved.
Craniosynostosis in a Painting by Hans Suess Kulmbach.

FIGURE 1. A. Triptych of the Rosary byt Hanse Suess Kulmbach ca 1510, Museo Thyssen–Bornemisza, Madrid, Spain. B. Detail of Triptych of the Rosary by Hanse Suess Kulmbach ca 1510, Museo Thyssen–Bornemisza, Madrid, Spain. non-verbal pattern, usually visual. Its main determinants are a good clinical knowledge, an extensive clinical experience and good observation skills. Indeed, iconodiagnosis is recognized as a valid way to training the skill of observation, and to improving empathy, and resilience among medical students. Here, iconodiagnosis was applied to a work by the German artist Hans Suess Kulmbach (1480–1522). Trained by Italian Renaissance artist Jacopo de’ Barbari, and German renaissance painter and printmaker Albrecht Dürer, he became famous for his paintings and his stained-glass windows. About 1510, Kulmbach depicted an oil on panel titled ‘‘Triptych of Rosary’’ (Fig. 1A) that is currently exposed at the Thyssen-Bornemisza museum in Madrid. It consists in three panels: the left panel represents the presentation of the Virgin in the Temple; on the right panel depicts the meeting at the golden gate; in the central panel there is a circular floral motif with the crosses indicating the 5 mysteries. Inside this rosary is the crucified Christ together with the Virgin, angels, the Blessed, and other figures. This painting points out the importance to reciting the rosary to obtain indulgences and salvation. Interestingly, the first figure, located under Christ’s left harm and representing Saint Peter, has a cranial malformation (Fig. 1B). His forehead presented with frontal bossing, and his skull has a long longitudinal diameter. These features may be compatible with a diagnosis of scaphocephaly, the most common form of craniosynostosis. More frequent in males, it is due to a premature fusion of the cranial sagittal suture, and results in a compensatory increasing of skull’s anteroposterior diameter, and sagittal suture’s enlargement. Based on the premature fusion’s extension, frontal bossing and occipital bulging may be visible. Intelligence and physical development are usually normal in nonsyndromic craniosynostosis. Moreover, the depicted man seems to have an auricle’s malformation and low set ears, maybe due to abnormal size of neurocranial compared with splanchnocranium. The ears’ low position leads to consider Muenke syndrome in differential diagnosis. However, Muenke syndrome is usually characterized by bilateral or unilateral coronal synostosis with consequent brachycephaly or plagiocephaly (although all sutures may be involved), widely spaced eyes, ptosis
A Feasible Laboratory-Strengthening Intervention Yielding a Sustainable Clinical Bacteriology Sector to Support Antimicrobial Stewardship in a Large Referral Hospital in Ethiopia

Background: Access to clinical bacteriology in low resource settings (LRS) is a key bottleneck preventing individual patient management of treatable severe infections, detection of antimicrobial resistance (AMR), and implementation of effective stewardship interventions. We sought to demonstrate the feasibility of a practical bundle of interventions aimed at implementing sustainable clinical bacteriology services at Tikur Anbessa Specialized Hospital in Addis Ababa, Ethiopia, and report on cost and intensity of supervision. Methods: Starting in Dec 2015, an intervention based on the CLSI QMS01-A guideline was established, consisting of (i) an initial needs assessment, (ii) development of key standard operating procedures, (iii) adaptation of processes for LRS, (iv) training and supervision of laboratory staff via consultant visits and existing online resources, and (v) implementation of a practical quality systems approach. A guiding principle of the bundle was sustainability of all interventions post implementation. Outcomes and challenges: An initial investment of ~US$ 26,200 for laboratory reagents, and a total of 50 visit-days per year from three Canadian and Norwegian microbiologists were committed. Twelve SOPs, including antimicrobial susceptibility testing, were adapted, and an automated blood culture platform was donated (bioMerieux). In the first 18 months of implementation of the intervention, the average volume of specimens analyzed in the lab went from 15/day to 75/day. The number of blood cultures tested increased from an average of 2/day to over 45/day. Antimicrobial susceptibility testing was introduced and cumulative antibiograms were generated for the institution. Quality control was implemented for all procedures and quality assurance tools implemented included external quality assurance and proficiency testing of six technologists with longitudinal follow-up. The laboratory is on the path toward SLIPTA accreditation by the African Society for Laboratory Medicine. Reagent costs, staff training and retention, and engagement of clinical personnel with the lab proved to be manageable challenges. Key external challenges include in-country supply-chain management issues, lack of competition among distributors, and foreign-currency exchange distortions. Conclusions: Using a relatively low-intensity intervention based on existing training tools and accreditation schemes, we demonstrate that establishment of reasonable-quality clinical bacteriology is not only within reach but also a critical step toward assessing the burden of AMR in settings like this one and implementing effective stewardship strategies.
Transition Bimetal Based MOF Nanosheets for Robust Aqueous Zn Battery

High-performance, good stability, low-cost, and environmentally friendly batteries are important for multifunctional electronics and electric vehicles. Compared with the high-energy density lithium-ion batteries, aqueous rechargeable battery has been extensively researched due to high safety, low cost and much better rate performance. Here, we report a one-step approach to fabricate porous Ni-Cu metal–organic framework (MOF) nanosheet arrays structures for stable energy storage with a high-energy (71.2 mWh cm−3) and high-stability (capacity retention of ≈91% after 2,500 cycles) performance. Furthermore, we synthesized various porous homogeneous Ni-Co, Ni-Zn, Ni-Fe and Ni-Mn bimetal MOF structures with high surface area and conductivity utilizing this rational design. This work provides a simple efficient strategy for constructing porous homogeneous bimetal MOF nanosheet arrays with high energy and stability performance, holding a great potential for future portable electronics.
Social impacts of living with chronic hepatitis B in South Sudanese community in Australia

ABSTRACT Introduction: Chronic hepatitis B presents significant issues to public health and clinical practices. The infection requires lifelong clinical management and is a leading risk factor for liver cancer and liver cirrhosis. Limited studies currently exist on the social impacts of living with chronic hepatitis B, especially for people from Sub-Saharan Africa. The current study explored the experiences of South Sudanese people living with chronic hepatitis B in Australia. Methods: A qualitative inquiry using face-to-face interviews with fifteen South Sudanese people with chronic hepatitis B in Australia explored the social impacts of living with chronic hepatitis B. The study purposively sampled participants who self-identified as South Sudanese with hepatitis B and over 18 years of age. Interviews were coded and analysed using thematic analysis. Results: The study identified three themes relating to the experiences of living with chronic hepatitis B, and were grouped into psychological, interpersonal and healthcare system levels. Psychological level experiences related to the uncertainty of living with chronic hepatitis B and liver cancer risk, where participants expressed persistent fear of disease progression and anxiety around death related to the disease. Interpersonal level experiences involved the disclosure of hepatitis B and its social impacts including stigma, social distancing and isolation. Healthcare system level experiences included receiving mixed messages about hepatitis B, challenges with regular testing and difficulty receiving hepatitis B treatment. Conclusion: The findings provide valuable insights into the social impacts of hepatitis B. It underlines the importance of integrated public health interventions at the community level to improve knowledge and awareness of hepatitis B which address stigma in the South Sudanese community in Australia.
Interactions of α2β1 integrin and its ligands, type I collagen and echovirus 1

Dadu, Elina Interactions of 2 1 integrin and its ligands, type I collagen and echovirus 1 Jyväskylä: University of Jyväskylä, 2015, 87 p. (Jyväskylä Studies in Biological and Environmental Science ISSN 1456-9701; 306) ISBN 978-951-39-6287-6 (nid.) ISBN 978-951-39-6288-3 (PDF) Yhteenveto: Kollageenireseptori 2 1-integriinin ja sen ligandien, tyypin I kollageenin ja echovirus 1:n, vuorovaikutukset Diss. Integrins are a large family of cell membrane receptors that attach cells to their surroundings. A subgroup of the integrins bind collagens, major constituents of extracellular matrix. Type I collagen molecules are the most abundant of them, forming large fibrils in the extracellular matrix. Here, the interactions of collagen receptor integrins and their ligands were investigated. The major type I collagen receptor, 2 1 integrin, was seen to be a functional receptor for type I collagen fibrils. In addition, the effect of integrins on collagen fibril formation was studied in vitro. The results suggest that integrins might participate in guiding collagen fibrillogenesis in vivo. In addition to collagen, echovirus 1 (EV1) also uses 2 1 integrin as a receptor. EV1 is a human pathogen and belongs to the Enterovirus B species. It enters cells via endocytosis and must release its genome and escape the vesicular endocytic structure before it can start the propagation of new virus particles. These early steps of EV1 life cycle were studied. Particularly, interactions of EV1 with lipid membranes and the effect 2 1 integrin has on these interactions were studied. Lipid membrane composition affected the affinity of virus particles to membranes. Intrestingly, flattening of EV1 particles was seen with the particles bound to membranes. The EV1 particles spread along the membranes and the spreading effect was lipid type specific. Presence of integrin enhanced the effect, suggesting that interactions of EV1 and integrin lead to changes in virus structure. These changes could include increased hydrophobicity of the virus surface and greater elasticity of the virus particle. In addition to lipid interaction studies, uncoating or genome release from EV1 particles was investigated using Raman spectroscopy. The observations suggest that uncoating leads to increased porosity of virus particles, and involves a reduction in the -helical content of the capsid proteins. In conclusion, this thesis reveals new information on interactions of collagen receptor integrins and their ligands. Novel features of virus-lipid interactions were discovered, and the Raman spectroscopic study of EV1 promotes method development in virology.
Interactive Gene Expression Patterns of Susceptible and Resistant Lens ervoides Recombinant Inbred Lines and the Necrotroph Ascochyta lentis

Ascochyta lentis is a foliar pathogen of Lens species and is of worldwide importance in cultivated lentil production. High levels of resistance were identified in the wild species Lens ervoides. This resistance was explored through histopathology, qPCR estimation of fungal biomass and transcriptome sequencing in a susceptible and a resistant recombinant inbred line (RIL) of L. ervoides infected with an aggressive isolate of A. lentis. Necrotrophic growth was delayed in the resistant RIL compared to accelerated necrotrophy of A. lentis in the susceptible RIL. Analysis of the fungal secretome indicated that the early activation of cell wall-degrading enzymes contributed to increased virulence of A. lentis. On the host side, gene co-expression analysis revealed that the invasion by A. lentis caused mRNA, DNA and protein decay in infected plants regardless of the level of resistance in the host. The resistant RIL exhibited a stronger gene co-expression in lipid localization and sulfur processes, and cellular responses to nutrients and stimuli than the susceptible RIL. In addition, differential gene analysis revealed that the repression of both, gibberellin signaling and cell death associated with the hypersensitive response (HR), were associated with enhanced A. lentis resistance.
Nanostructural Analysis of Enzymatic and Non-enzymatic Brown Rot Fungal Deconstruction of the Lignocellulose Cell Wall†

Brown rot (BR) decay mechanisms employ carbohydrate-active enzymes (CAZymes) as well as a unique non-enzymatic chelator-mediated Fenton (CMF) chemistry to deconstruct lignocellulosic materials. Unlike white rot fungi, BR fungi lack peroxidases for lignin deconstruction, and also lack some endoglucanase/cellobiohydrolase activities. The role that the CMF mechanism plays in “opening up” the wood cell wall structure in advance of enzymatic action, and any interaction between CMF constituents and the selective CAZyme suite that BRs possess, is still unclear. Expression patterns for CMF redox metabolites and lytic polysaccharide monooxygenase (LPMO–AA9 family) genes showed that some LPMO isozymes were upregulated with genes associated with CMF at early stages of brown rot by Gloeophyllum trabeum. In the structural studies, wood decayed by the G. trabeum was compared to CMF-treated wood, or CMF-treated wood followed by treatment with either the early-upregulated LPMO or a commercial CAZyme cocktail. Structural modification of decayed/treated wood was characterized using small angle neutron scattering. CMF treatment produced neutron scattering patterns similar to that of the BR decay indicating that both systems enlarged the nanopore structure of wood cell walls to permit enzyme access. Enzymatic deconstruction of cellulose or lignin in raw wood samples was not achieved via CAZyme cocktail or LPMO enzyme action alone. CMF treatment resulted in depolymerization of crystalline cellulose as attack progressed from the outer regions of individual crystallites. Multiple pulses of CMF treatment on raw wood showed a progressive increase in the spacing between the cellulose elementary fibrils (EFs), indicating the CMF eroded the matrix outside the EF bundles, leading to less tightly packed EFs. Peracetic acid delignification treatment enhanced subsequent CMF treatment effects, and allowed both enzyme systems to further increase spacing of the EFs. Moreover, even after a single pulse of CMF treatment, both enzymes were apparently able to penetrate the cell wall to further increase EF spacing. The data suggest the potential for the early-upregulated LPMO enzyme to work in association with CMF chemistry, suggesting that G. trabeum may have adopted mechanisms to integrate non-enzymatic and enzymatic chemistries together during early stages of brown rot decay.
The Impact of Specialized Corpora for Word Embeddings in Natural Langage Understanding

Recent studies in the biomedical domain suggest that learning statistical word representations (static or contextualized word embeddings) on large corpora of specialized data improve the results on downstream natural language processing (NLP) tasks. In this paper, we explore the impact of the data source of word representations on a natural language understanding task. We compared embeddings learned with Fasttext (static embedding) and ELMo (contextualized embedding) representations, learned either on the general domain (Wikipedia) or on specialized data (electronic health records, EHR). The best results were obtained with ELMo representations learned on EHR data for the two sub-tasks (+7% and +4% of gain in F1-score). Moreover, ELMo representations were trained with only a fraction of the data used for Fasttext.