Miracle-dz/UniFAR-FTUs huggingface dataset:
Low‐Temperature‐ and Phosphate Deficiency‐Responsive Elements Control DGTT3 Expression in Chlamydomonas reinhardtii

acyl‐CoA:Diacylglycerol acyltransferases (DGAT) catalyse the final step of the triacylglycerol biosynthesis. Two major gene families have been shown to encode DGATs, DGAT1, and DGAT2. Abiotic factors such as low temperatures, nitrogen, or phosphorus deficiency was reported to play important roles in the growth and development in green algae. Whether DGATs are induced by low temperatures or phosphorus deficiency, and the corresponding promoter elements are not reported yet. In this study, we found DGTT3 to have a significant response to low temperatures, phosphorus deficiency, and other stresses, such as high concentrations of NaCl, 20 μM GA, and 20 μM abscisic acid. The promoter element of DGTT3 was then studied by deletion and scanning mutagenesis method. Results revealed that the − 319/− 247 region is essential for low‐temperature and phosphate‐deficiency‐mediated induction of DGTT3 expression. The sequence from − 312 to − 299 of the CAATAGACTGCTGCT was the core sequence of the cold responsive element, which facilitated the promoter response to cold induction. Meanwhile, the sequence from − 319 to − 275 was critical to phosphate‐deficiency regulation. Furthermore, the relationship between DNA methylation and transgenic silence in −N condition was analyzed, and results showed that the DNA methylation rate of the transformed insertion region was high. This phenomenon was responsible for the decrease in ARS gene expression in the transgenic algal strain under −N conditions.
BACTERIAL DIVERSITY OF Fe / Mn & WHITE ROCK COATINGS IN KÄRKEVAGGE : A POTENTIAL MARS ANALOGUE

Introduction: Kärkevagge is a region in the Swedish lapland which is defined by a 5km valley created by glacial processes. Kärkevagge exhibits a low mean annual temperature of -2 o C, low precipitation of below 900 mm annually, an acidic environment, and a mineralogy primarily dominated by sulfate and iron [1]aspects which make the region a well-rounded analogue for the Martian landscape. Rock coatings are of particular interest as Martian biosignatures. Rock coatings provide UV protection from the harsh Martian atmosphere, would preserve biosignatures past the longevity of the organisms, and are, unlike subterranean environments, accessible by rover. The region is host to a variety of rock coatings and weathering rinds. Iron-rich weathering rinds, Fe/Mn films, silican and alumina glazes, sulfate crusts, and heavy metal skins are found in the valley [2]. The rock coatings focused on currently in this project are Fe/Mn skins, white coatings composed of alumina glaze with iron and silicon, and sulfate crusts. Microbial activity is capable of influencing geological formations [3]. Through cataloguing the microbial diversity in the rock coatings and understanding the relationship between microbes and the mineralogy on rock coatings, the potential application of rock coatings as biomarkers for life on the Martian landscape can be advanced. Methods: Rock coatings were fragmented and crushed. Microbial DNA was exctracted from crushed coating samples using PowerSoil® DNA Isolation Kit from MoBio (Carlsbad, CA) according to manufacturer protocols. Isolated DNA was mixed with GoTaq® Green Master Mix from Promega (Madison, WI) and universal forward and reverse primers for PCR amplification. To specifically amplify the bacterial 16S rDNA genes, 533-forward universal (5’-GTG CCA GCC GCC GCG GTA A-3’) and 1492-reverse universal (5’-GGT TAC CTT GTT ACG ACT T-3’) primers were used. The thermocycler was programmed for an initial denaturing of 5 min at 94 o C and 35 cycles of 1 min at 94 o C, 45 sec at 47 o C, & 1 min at 72 o C, with a final extension of 7 min at 72 o C. DNA fragments of the amplified products were purified using ethanol and sodium acetate then cloned into the pSC-A cloning vector and transformed into E. coli cells using StrataClone PCR Cloning Kit from Agilent Technologies (Santa Clara, CA) per manufacturer protocol. The transformed cells were plated on LB-ampicillin media and incubated for no more than 20 hours. A minimum of 24 positive colonies were randomly selected from plates, with DNA fragments amplified using the M13 -20 forward and M13 reverse primers. PCR products were then sent to Functional Bioscences (Madison, WI) for purification and sequencing using the T7 universal primer. Sequences were compared to the BLAST nucleotide database. The highest % matches were further analyzed for their isolate source and physiology.
Peri-operative chemotherapy for muscle-invasive bladder cancer: status-quo in 2017

The role of perioperative chemotherapy associated with radical cystectomy (RC) for muscle-invasive bladder cancer has been analyzed in several landmark randomized controlled trials (RCTs) over the past decades. With regard to neoadjuvant chemotherapy (NAC), a meta-analysis of level 1 evidence and long-term results from the largest RCTs support its use, which is currently advocated as the standard of care by most of the clinical guidelines worldwide. However, with regard to the delivery of adjuvant chemotherapy (AC), evidence is more contentious. Specifically, several meta-analyses demonstrated a survival benefit associated with the use of cisplatin-based regimen but investigators identified multiple methodological limitations in most of included RCTs. Nonetheless, AC is currently considered for fit patients with adverse pathological features at RC. It is noteworthy that the delivery of such cytotoxic treatment after surgery may maintain significant anti-tumor activity even in those patients who previously received NAC. Finally, given its greater response rate, the methotrexate, vinblastine, adriamycin plus cisplatin combination remains preferentially considered in the neoadjuvant setting, while the gemcitabine plus cisplatin combination is more commonly delivered in the adjuvant setting because of its better toxicity profile. However, no prospective evidence comparing efficacy of both regimens for NAC or AC is currently available.
Neutralization of bovine papillomavirus by antibodies to L1 and L2 capsid proteins

We have generated four mouse monoclonal antibodies (MAbs) to bovine papillomavirus virions that bound type-specific, adjacent, and conformationally dependent epitopes on the L1 major capsid protein. All four MAbs were neutralizing at ratios of 1 MAb molecule per 5 to 25 L1 molecules, but only three effectively blocked binding of the virus to the cell surface. Therefore, antibodies can prevent papillomavirus infection by at least two mechanisms: inhibition of cell surface receptor binding and a subsequent step in the infectious pathway. The neutralizing epitopes of the bovine papillomavirus L2 minor capsid protein were mapped to the N-terminal half of L2 by blocking the neutralizing activity of full-length L2 antiserum with bacterially expressed peptides of L2. In addition, rabbit antiserum raised against amino acids 45 to 173 of L2 had a neutralizing titer of 1,000, confirming that at least part of the N terminus of L2 is exposed on the virion surface.
Development of Scales Based on Patterns of Drug Effects, Using the Addiction Research Center Inventory (ARCI)

A series of scales were developed on the basis of the pattern of change of responses on Addiction Research Center Inventory items produced by drugs including morphine, pentobarbital, chlorpromazine, alcohol, LSD, pyrahexyl, and amphetamine in post addicts. The pattern scales were compared with empirically developed scales that measure the effects of each drug as contrasted with placebo. It was found that the empirical scales show a greater sensitivity to general or non-specific drug effects than pattern scales, i.e., all drugs in the series produced significant elevations on empirical scales. Because of this characteristic, less differentiation between drugs is possible with empirical scales. On scales which reflect patterns of drug actions, greater differentiation between drugs was shown. Higher doses produced more specific drug effects than lower doses. This difference was produced probably by a relatively greater contribution of non-specific drug effects for lower doses. Significant reliability coefficients were obtained for all scales. Reliability of scales across conditions was related to the type of scale and similarity of conditions. As indicated by several findings, condition-similarity has implications for relating personality to drug effects.
Modelling of household electro-thermal technologies for demand response applications

With increasing penetration of renewable and low carbon energy resources and electrification of energy consumption, Demand Response (DR) is expected to play a more important role in system balancing, network capacity support, and wholesale electricity markets. However, the side effects of DR control could cause various issues that could eventually hinder its deployment. Therefore the impact of DR needs to be properly understood and modelled. A potentially significant source of DR may be found in control of electro-thermal technologies (ETT, i.e. electric heat pump and micro combined heat and power units) installed in the domestic sector, which could help to alleviate low voltage network congestion. In this respect, this paper introduces a detailed physical model which is used to simulate, with high resolution, the load shapes of different ETT in different buildings with different thermal inertia characteristics. Simulation studies are carried out to evaluate the changes in load patterns in the different cases and the impact of possible DR control strategies, also taking into account the thermal comfort level of occupants. The high resolution model developed aims at giving insights on the expected load pattern changes when applying different DR control schemes to different ETT and in different types of houses.
Understanding the key factors that control the rate of β-hairpin folding

Both turn sequence and interstrand hydrophobic side-chain–side-chain interaction have been suggested to be important determinants of β-hairpin stability. However, their roles in controlling the folding dynamics of β-hairpins have not been clearly determined. Herein, we investigated the structural stability and folding kinetics of a series of tryptophan zippers by static IR and CD spectroscopies and the IR temperature jump method. Our results support a β-hairpin folding mechanism wherein the rate-limiting event corresponds to the formation of the turn. We find that the logarithm of the folding rate depends linearly on the entropic change associated with the turn formation, where faster folding correlates with lower entropic cost. Moreover, a stronger turn-promoting sequence increases the stability of a β-hairpin primarily by increasing its folding rate, whereas a stronger hydrophobic cluster increases the stability of a β-hairpin primarily by decreasing its unfolding rate.
Effects of temperature on electrical parameters in GaAs SOI FinFET and application as digital inverter

In this paper, a GaAs SOI (Silicon on Insulator) FinFET is proposed. Si FinFET is suitable for high speed applications, but GaAs FinFETs provides better performance than Si FinFET. The impact of temperature on electron mobility, drain current, and Ion/Ioff have been reported to realize the behaviour of the device. TCAD simulation shows that as temperature raises Ion reduces, Ioff increases, and carrier mobility decreases. Also the effect of temperature on short channel effects (SCEs) has been studied. Results show that as temperature increases SS reduces, DIBL decreases, and also Vt roll off occur. An investigation on the impacts of temperature on gate capacitance (Cgg) and intrinsic delay (τ) have been presented. The value of Cgg and intrinsic delay increases as temperature increases. We implemented a CMOS inverter through proposed FinFET and the effect of temperature on its characteristic is reported. Average delay increases as temperature increases.
Turkey ovomucoid third domain inhibits eight different serine proteinases of varied specificity on the same ...Leu18-Glu19 ... reactive site.

We show that eight different serine proteinases--bovine chymotrypsins A and B, porcine pancreatic elastase I, proteinase K, Streptomyces griseus proteinases A and B, and subtilisins BPN' and Carlsberg--interact with turkey ovomucoid third domain at the same Leu18-Glu19 peptide bond, the reactive site of the inhibitor. Turkey ovomucoid third domain was converted to modified (the reactive site peptide bond hydrolyzed) form as documented by sequencing. Complexes of all eight enzymes both with virgin and with modified inhibitor were prepared. All 16 complexes were subjected to kinetically controlled dissociation, and all 16 produced predominantly virgin (greater than 90%) inhibitor, thus proving our point. During this investigation, we found that both alpha-chymotrypsin and especially S. griseus proteinase B convert virgin to modified turkey ovomucoid third domain, even in the pH range 1-2, a much lower pH than we expected. We have also measured rate constants kon and kon* for the association of virgin and modified turkey ovomucoid third domain with several serine proteinases. The kon/kon* ratio is 4.8 X 10(6) for chymotrypsin, but it is only 1.5 for subtilisin Carlsberg. A number of generalizations concerning reactive sites of protein proteinase inhibitor are proposed and discussed.
Carbon metabolism and transport in arbuscular mycorrhizas.

Colonization of the land by plants some 400 million years ago was associated with the colonization of their primitive roots by soil-borne filamentous fungi ([Nicolson, 1975][1];[Simon et al., 1993][2]; [Taylor et al., 1995][3]). Today, 90% to 95% of land plants still maintain some type of
Neurocontrollers for Trajectory Tracking Problem of a Nonholonomic Mobile Robot

Abstract In this paper, a trajectory tracking control for a nonholonomic mobile robot by the integration of a kinematic controller and a torque controller is investigated. The proposed torque controllers (PTCs) are based on a Gaussian radial basis function neural network (RBFNN) modeling technique, which are used to compensate the mobile robot dynamics, significant uncertainties and disturbances. Also, the PTCs are not dependent of the robot dynamics neither requires the off-line training process. The stability analysis and the convergence of tracking errors to zero, as well as the learning algorithms (for weights, centers, and widths) are guaranteed with basis on Lyapunov's theory. In addition, the simulation results shows the efficiency of the PTCs.
Safety Design of Ore Transfer Facilities in Steel Works

In this paper, safety design of ore transfer facilities in steel works is studied. For ore yard of iron and steel works, it is necessary to supply required amount of iron ore via multiple numbers of conveyers. To attain the stable operation of ore transfer, the optimized usage of transfer facilities for continuous transfer. The equalized load distributions of conveyer operations are preferable for safety transfer operations preventing facility stop by fault of the specified facility. A new method of safety design of transfer facilities is developed based on a decentralized agent model. Simulation results by decentralized agent method revealed existence of the appropriate arrangement of the transfer facilities
Development and pilot testing of a text message vaccine reminder system for use during an influenza pandemic

ABSTRACT In an influenza pandemic, two vaccine doses administered 21 days apart may be needed for individuals of all ages to achieve seroprotection. Achieving dose-series completion can be challenging even for routinely recommended vaccines. To prepare for a two-dose influenza pandemic vaccine campaign and promote dose-series completion and correct dosing intervals, CDC and its partners developed a text message-based vaccine reminder system to remind persons who receive a first dose of pandemic influenza vaccine to receive the second dose. Taking advantage of the high prevalence of cell phones in the United States, the system sent second-dose text message reminders and hyperlinks to educational information. The system was pilot tested from November 2015 to April 2016 among graduate public health students enrolled at four United States universities. Universities were selected based on convenience, and each university used a different recruitment method. Among 59 volunteers who pilot tested the system and completed a survey, 57 (92%) felt the system would be helpful during a pandemic. Forty (68%) respondents felt the information included in the messages was informative. Volunteers recommended including actionable ways to stay healthy during a pandemic, though specific suggestions varied. With further development, text reminder systems could be used to promote adherence to a two-dose regimen in a future pandemic, although audience-specific messaging and other complementary systems will likely be needed. Public and private partners can adapt and implement this tool in conjunction with their routine patient information systems to improve dose-series completion and ensure optimal protection during an influenza pandemic.
Electric field in polymeric cable due to space charge accumulation under DC and temperature gradient

When power cables are loaded under high voltage direct current (HVDC), an accumulation of space charge and a radial distribution of temperature gradient are developed across the insulation material. Such existence and accumulation of space charge within the insulating material poses a threat to the reliability of the operation of dc power cables. The electric field of a practical dc power cable is affected by the conductivity of the material, which is a function of both temperature and electric field. This causes difficulties in identifying the electric field distribution. In this paper, a method of determining the electric field distribution in dc power cables was proposed by considering the influence of space charge on the conductivity of the insulating material under different temperatures. Commercial 11 kV ac cross-linked polyethylene (XLPE) power cables were used and the space charge in these cables under dc conditions was measured using a modified pulsed electroacoustic (PEA) system with an attached current transformer. Therefore, a replica of a power cable under load conditions is obtained, which allows an investigation of the formation, migration and accumulation of space charge in a power cable with and without temperature gradients across the insulating material. COMSOL Multiphysics software package was used to accurately determine the electric field distribution in the dc power cable with consideration of the influence of electric field on the conductivity of the insulating material. The numerical modelling is based on the hopping conduction mechanism and its parameters were obtained from experiments carried out on the XLPE insulation material.
Real-time context-aware and personalized media streaming environments for large scale broadcasting applications My-e-Director 2012

This paper presents the architecture of the My-e-Director 2012, an FP7 call-1 European funded project in the objective area of Networked Media. My-e-Director 2012, is a unique interactive broadcasting platform, which enables end-users to have access on innovative services, like selection of focal actors/scenes and points of interest within real-time broadcasted streams.
Theoretical Model of Multipurpose Land Information Systems Development

Historically, multipurpose land information systems have often been designed for use as community‐wide enterprises. Before becoming useful to the community, however, many of these systems encounter prohibitive factors delaying or preventing the satisfaction of the ultimate goals for which they were designed. A criticism of current models is that system developers seeking to build publicly accessible systems do not use them. Since most systems are developed without the guidance of a formal model, system developers often do not fully anticipate likely system issues. Many systems, designed on an ad hoc basis, fail to achieve their full potential, because they have not used an appropriate model to determine a final system outcome. This paper presents a theoretical model describing the stages systems experience during development. These stages are: no development, system initiation, database development, recordkeeping, analysis, and democratization. Additionally, the factors promoting or prohibiting movement from one stage to the next, and the indicators or outcomes of each stage are described. Not only are the individual elements described but also their relationships are described as part of the model. The Multipurpose Land Information System (MPLIS) development model is designed both as a guide for system developers and a tool for discussing and comparing systems.
The Nursing Couple

M.D. Hamish Hamilton, Ltd. Fp. iyx 7s. 6d. This book is quite unique in its approach to the subject of breast feeding. The title shows first the emphasis that the author places on the mutual influence both partners in breast feeding exercise on one another, indeed she shows throughout the book that breast feeding, if successful, is always the result of a mutual adaptation of mother and babe to one another.
Affordance Feature Reasoning in Some Home Appliances Products

Interactions between human and objects are made through specific features of the object, which are adequate to the task context. During interactions, the perceived features vary according to the context. Affordances are the messages that products provide and users perceive in such a way that user actions are naturally induced with the help of such messages. In this research, we define affordance features as structural elements providing affordances. We classify affordance features into functional, ergonomics, and informative aspects. For some home appliances, common affordances and affordance features are identified so that they can be used in designing other products.
Water equivalent thickness values of materials used in beams of protons, helium, carbon and iron ions

Heavy charged particle beam radiotherapy for cancer is of increasing interest because it delivers a highly conformal radiation dose to the target volume. Accurate knowledge of the range of a heavy charged particle beam after it penetrates a patient's body or other materials in the beam line is very important and is usually stated in terms of the water equivalent thickness (WET). However, methods of calculating WET for heavy charged particle beams are lacking. Our objective was to test several simple analytical formulas previously developed for proton beams for their ability to calculate WET values for materials exposed to beams of protons, helium, carbon and iron ions. Experimentally measured heavy charged particle beam ranges and WET values from an iterative numerical method were compared with the WET values calculated by the analytical formulas. In most cases, the deviations were within 1 mm. We conclude that the analytical formulas originally developed for proton beams can also be used to calculate WET values for helium, carbon and iron ion beams with good accuracy.