Miracle-dz/UniFAR-FTUs huggingface dataset:
Computational analysis of filament polymerization dynamics in cytoskeletal networks

The polymerization–depolymerization dynamics of cytoskeletal proteins play essential roles in the self-organization of cytoskeletal structures, in eukaryotic as well as prokaryotic cells. While advances in fluorescence microscopy and in vitro reconstitution experiments have helped to study the dynamic properties of these complex systems, methods that allow to collect and analyze large quantitative datasets of the underlying polymer dynamics are still missing. Here, we present a novel image analysis workflow to study polymerization dynamics of active filaments in a non-biased, highly automated manner. Using treadmilling filaments of the bacterial tubulin FtsZ as an example, we demonstrate that our method is able to specifically detect, track and analyze growth and shrinkage of polymers, even in dense networks of filaments. We believe that this automated method can facilitate the analysis of a large variety of dynamic cytoskeletal systems, using standard time-lapse movies obtained from experiments in vitro as well as in the living cell. Moreover, we provide scripts implementing this method as supplementary material.
Application of gonadotropic releasing hormone antagonist in the prevention and treatment of ovarian hyperstimulation syndrome

Objective To investigate the effectiveness of the prevention and treatment with gonadotropic releasing hormone antagonist(GnRH ant) on OHSS patients who underwent in vitro fertilization and embryo transfer(IVF-ET). Methods In this study, fresh transplantation was cancelled(the number of oocytes retrieved≥ 20, serum E2 level4000 pg/ml on the day of HCG injected) in total 44 patients who subjected to IVF-ET. GnRH antagonist group of 21 patients were given cetrorelix acetate 0.25 mg daily. Control group of 23 patients received the same treatment protocol except the GnRH antagonist. Serum E2 level was examined and the prevalence of OHSS, moderate and severe OHSS was calculated. Results Serum E2 level in two groups were all decreased gradually. However, E2 level of the antagonist group on third, fifth, seventh day after treatment was lower than that of the control group(P0.05). The ratio of non-OHSS in antagonist group significantly higher than that in the control group(42.9% vs. 13.0%, P=0.027), while morbidity of the severe OHSS in antagonist group was significantly lower than that in the control group(23.8% vs. 69.6%, P=0.001). Conclusions Gonadotropic releasing hormone antagonists can decrease the incidence of OHSS and reduce the deterioration of OHSS. GnRH antagonist administration might represent a new approach for the effective prevention and treatment of OHSS.
Redescription of two overlooked species of the Perinereis nuntia complex and morphological delimitation of P. nuntia (Savigny in Lamarck, 1818) from the Red Sea (Annelida, Nereididae)

ABSTRACT Two poorly-known species are systematically studied in detail: Nereis (Nereis) latipalpa Schmarda, 1861 from Cape Town, South Africa, and Nereis (Neanthes) larentukana Grube in Peters, 1881 from Larantuka, Flores, Indonesia. Both are raised from synonymy and transferred to the Perinereis nuntia species complex based upon the revision of type material using characters already known, novel, or even forgotten in the literature. Perinereis latipalpa n. comb. is different from its previous senior synonym P. vallata (Grube & Kröyer in Grube, 1858) and related species. The type or topotype specimens of three other nereidid species share the same relevant features as P. latipalpa n. comb. ; these are herein regarded as junior synonyms: Neanthes latipalpa Kinberg, 1865, Neanthes latipalpa typica Willey, 1904, and Perinereis namibia Wilson & Glasby, 1993. Perinereis larentukana n. comb. is redescribed and compared to specimens of the morphologically similar species P. nuntia (Savigny in Lamarck, 1818). An improved characterization of P. nuntia using Red Sea specimens similar to the type description was also performed in order to clarify its morphology and restrict the species-complex name content. Other synonymized species of the P. nuntia complex were recognized as distinct: Perinereis brevicirris Grube, 1866, P. quatrefagesi Grube, 1878, and P. weijhouensis Wu, Sun & Yang, 1981. This was based upon features not considered in the previous revisions but pointed out in the original descriptions. An identification key to the 20 currently valid species within the Perinereis nuntia complex is also provided.
Effect of Allium cepa (Onion) Powder on the Growth and Survival in Cirrhinus mrigala Fingerlings

The experiments were conducted using feed prepared with supplementation of onion powder to know their effect on growth performance and survival rate in Cirrhinus mrigala fingerlings (2.65 ± 0.02g). Fingerlings were stocked at a density of 10 nos./experimental tank. Experimental diets were formulated with 35% protein level. T0 diet was considered as control. Onion were added at the rate of 5g, 10g, 20g and 50g per kg of feed in T1, T2, T3 and T4 respectively. The experimental feed was given at the rate of 10% of body weight of the fish twice daily. After 10 days, feeding was decreased to 4% of body weight. After 60 days, Results showed that mean weight gain (%), SGR, PER and survival were significantly higher in T2. The significantly lowest FCR was recorded in T2 (2.65± 0.03) respectively. The survival rate was higher in T2 (97± 0.70), but it was not significantly different among treatments. The results of the present investigation revealed that the supplementation of onion in the diet of C. mrigala fingerlings significantly affected the mean weight gain (%), SGR, PER and survival rate. However, there was significant difference in the survival rate of C. mrigala fingerling among the treatments.
Oct2, BCL6, IRF8, OCAB and PU.1 in the Assessment of Prognosis in Diffuse Large B cell Lymphoma Patients

Background: Diffuse Large B-Cell Lymphoma (DLBCL) is the most common type of Non-Hodgkin Lymphoma in adults. This germinal center derived B cell lymphoma is a heterogeneous disease with a highly variable clinical course, currently treated with immune-chemotherapy. The International Prognosis Index (IPI) remains the main prognosis indicator. This highlights the absence of biomarkers suitable to provide molecular biology information to more accurately establish prognosis and predict treatment response in DLBCL patients. Methods: We determined the Oct2, BCL6, IRF8, OCAB and PU.1 transcription factors expression by immunohistochemistry in 73 DLBCL lymph node biopsies to address their potential as prognosis biomarkers in DLBCL patients. These molecules exhibit well-known key roles in the germinal center development. Results: A large number of cases showed high Oct2 (64/73), BCL6 (40/73) and/or IRF8 (44/73) percentage of positive tumor cell nuclei. In contrast, a significant number of analyzed biopsies, showed a low OCAB and/or PU.1 percentage of positive tumor cells. The expression of each factor was not associated with any of the relevant clinical-pathological features including the DLBCL molecular subtype and the IPI. Oct2, BCL6 and IRF8 high expression (more than 70% of positive tumor cells) correlated with poor prognosis in terms of shorter overall survival. Particularly, high BCL6 and IRF8 expression maintained their prognostic value in a multivariate analysis stratified for the IPI score. Interestingly, IRF8 emerged as a novel prognosis indicator among the free bone marrow disease patients at diagnosis, subjected to a specific multivariate analysis named classification tree. Patients with free-bone marrow disease, which normally have a better outcome, showed a worse prognosis when they expressed high IRF8 at diagnosis. Conclusions: The assessment of these factors expression would provide novel cellular and molecular insights to more efficiently predict DLBCL patient prognosis.
Rabies virus: A mini review

Rabies is the disease caused by the rabies virus belong to the family of rhabdoviridae that is mostly transmitted by the bite of dog and show different dangerous symptoms like hydrophobias, malaise, irregular breathing and consequently death. It is bullet shaped virus with small RNA genome that encode five proteins that are responsible for the pathogenicity. G protein have main role in pathogenicity. It attack the central nervous system by using network of nerves cells, hijack it and starts transcribing itself. There is no cure for rabies but it will be avert by preventive measures. The vaccination of dogs are first step to prevent rabies virus.
Evaluation of Biochemical and Heamatological Changes in Plasmodium-berghei-Infected Mice Administered With Aqueous Extract of Tithonia diversifolia

This work was done to investigate the biochemical and haematological parameters of aqueous extract of Tithonia diversifolia plant, in mice induced with Plasmodium berghei a plant that is used locally to treat malaria. A total of 25 mice were used in this study and were divided into 5 groups. Group A (control), group B group C, group D and group E were infected with Plasmodium bergei and treated with normal saline, artesunate (50mg/kg), 300mg/kg and 600mg/kg body weight of the extract respectively. MCH – mean cell hemoglobin; MCHC – mean cell hemoglobin concentration; MCV– mean cell volume; PCV – packed cell volume; PLT – platelet count; RBC – red blood cells; WBC – white blood cell count/leukocyte count , N – Neutrophil Count, L-Lymphocyte Count, HbHemoglobin count, Mmonocyte count and AST –Aspartate Aminotransferase , ALTAlanine Aminotransferase and ALP Alkaline phosphatase were evaluated and analysed statistically. The results revealed that groups treated with 300mg/kg, 600mg/kg Tithonia diversifolia leave extract and artesunate had reduced parasitaemia level of 76.69%, 83.02% and 84.72% respectively. The administration of (300mg/kg) dose of the extract and artesunate (50mg/kg) caused a decrease in the activities of AST, ALT and ALP that may indicate safety while administration of 600mg/kg dose of the extract caused an increase, this may suggest that 600mg/kg dose exposure of the extract could pose a risk resulting to organ damage. The administration 300mg/kg cause an increase in Hb, PCV, WBC , N with decrease in PLT while a decrease was observed for the Hb, PCV with significantly increase in WBC of group administered with the 600mg/kg dose of the extract when compared with the negative control. It was concluded that although T. diversifolia is reported to have health benefit as it offer treatment and protection against malaria, the observed changes in biochemical and heamatological parameters suggest that it may have some clearly definable safety and toxicity properties especially at 300mg/kg and 600mg/kg dose in Plasmodium berghei infected mice.
Comparison of fine-scale spatial distribution and nest-site selection in great crested grebe ( Podiceps cristatus ) and coot ( Fulica atra )

Ecologists suggest that organization of animal community is determined by the interaction among species in a given area (MacArthur, 1970; May, MacArthur, 1972; Roughgarden, 1974; Toft , 1982; Begon et al., 1989). Such approach is inevitably related to the problem of resource partitioning. Th e patterns of such partitioning can be recognized as non-random diff erences in the use of space and food (Schoener, 1974; Werner, 1977; Toft , 1980; Toft , 1982; Dunham, 1980; Arlettaz et al., 1997; Karlson et al., 2007). With a large overlap in the breeding timing and nesting sites and no competition for food resources, as in the case of great crested grebe and coot, an ecologically meaningful and delicate task is to fi nd out how “nesting habitat resources” are shared. However, we failed to fi nd comparative data on nest-site selection by great crested grebe and coot. It seems that the behavioral aspect of spatial interaction between coot and great crested grebe is a more popular object of investigation (Блум, 1973; Кошелев, 1984; Goc, 1986; Konter, 2002; Stanevičius, Švažas, 2005) than comparison of their habitat characteristics. Ecological separation and microhabitat selection of American Coot and some American native grebe species were compared by Nudds (1982). In our paper, we explored diff erences in nest-site choice and spatial distribution between great crested grebe and coot on wetlands where both species are common. To achieve this goal, we investigated: (i) whether the patterns of nest distribution over plant patches diff er between the species, (ii) what is the degree of spatial isolation and overlap in the use of plant patches by the species, (iii) diff erences in some habitat characteristics between patches occupied by great crested grebe only, by Coot only and by both species, (iv) diff erences in nest-site characteristics between the species. MATERIALS AND METHODS
The production performance of Holstein and Fleckvieh x Holstein cows in an intensive feeding system

Dairy producers are finding dairy farming challenging due to high input costs and low farm gate milk prices. A major constraint is the longevity of dairy cows mainly because of poor fertility. Crossbreeding is currently being considered as an option to overcome poor fertility in dairy cows. The Fleckvieh dual-purpose breed is a Simmental derived breed from Germany with medium to high milk and fertility characteristics. In this study the milk production performance of 150 Holstein (H) and 139 Fleckvieh x Holstein (FxH) cows in a total mixed ration feeding system was compared. Cows were fed a total mixed ration in dry lots and were milked twice a day. Milk, fat and protein production, fat and protein percentages were determined over consecutive lactation periods according to standard milk recording procedures. Production parameters corrected for a 305-day lactation period did not differ (P>0.05) between genotypes, being 6330±117 vs. 6108±97 kg milk, 252±4.7 vs. 251±3.9 kg fat, 202±3.5 vs. 200±2.9 kg protein for H and FxH cows, respectively. Fat and protein percentages differed (P<0.05) between genotypes, being 3.98±0.03 vs. 4.13±0.02% and 3.20±0.02 vs. 3.30±0.02 for H and FxH cows, respectively. Using a dual-purpose breed in a crossbreeding programme on Holsteins did not reduce the milk yield of crossbred cows. Generally results agree with research conducted in the USA also showing no differences in milk yield parameters between Montbéliarde x Holstein in comparison to Holstein cows.
Effects of tongxinluo on C-reactive protein and clinical prognosis in patients after coronary stenting

Objective To investigate the effects of Tongxinluo on C reactive protein(CRP)and clinical prognosis in patients after coronary stenting. Methods From January 2003 to December 2004, 132 patients in our department diagnosed as coronary artery disease(including acute myocardial infarction, unstable angina pectoris and stable angina pectoris)were divided into two groups: Tongxinluo group and control group. The control group received routine treatment, while Tongxinluo group based on routine treatment was administrated with Tongxinluo capsule in combination for 6 months. Results CRP level was much lower in Tongxinluo group together with apparent decrease of the major adverse cardiac event(MACE)rate in 6 months' follow-up than those of the control group, but showing no difference in coronary arterial restenosis between the two groups at 6 months after coronary stenting. Conclusion Tongxinluo has favorable effects to decrease the CRP level and improve clinical efficiency together with prognosis for patients after coronary stenting.
Akut pulmoner emboli

SUMMARy Acute pulmonary embolism Pulmonary embolism (PE) is a common and life-threatening emergency cardiovascular disorder. PE does not occur with a specific clinical picture, so the diagnosis can be difficult. Early diagnosis and treatment is crucial to save lives. PE an deep vein thrombosis (DVT) is associated with venous thromboembolism (VTE) and have common predisposing factors. In most cases, Cause of PE commonly is DVT. Among the factors that cause PE include age, history of VTE and such as cancer, neurological disease, acute respiratory failure or heart disease that requires long hospitalization diseases, oral contraceptive use and congenital or acquired thrombophilia. Smokers, obese people, patients with hypertension or metabolic syndrome has been reported that a high risk of PE. Increases plasma level of D-dimer that fibrin degradation product. The venous ultrasound of lower extremity is first step tests in patients with suspected PE. Because it's noninvasive, easy to apply and has a high specificity. Normal ventilationperfusion (V/Q) scintigraphy is a highly secure PE outside. Single-detector spiral CT, is sufficient in showing the presence of segmental or proximal level clinically apparent PE. Pulmonary angiography is extremely reliable, but is an invasive test. Critical condition in which the patients with suspected PE echocardiography at the beginning of beds, especially the granting of emergency treatment is useful for decision.
Northward migration of trembling aspen will increase growth but reduce resistance to drought-induced xylem cavitation

Tree migration to higher latitudes may occur in response to future changes in climate, exposing the trees to higher concentrations of carbon dioxide ([CO2]), new photoperiods, different levels of soil moisture, and other new conditions. These new conditions can influence the physiology, survival, and growth of trees. This study examined the interactive effects of [CO2], photoperiod, and soil moisture on the morphology and resistance to xylem cavitation in trembling aspen (Populus tremuloides Michx.). One-year-old seedlings, in greenhouses, were exposed to two [CO2] (ambient [CO2] 400 μmol·mol−1 or an elevated [CO2] 1000 μmol·mol−1), four photoperiod regimes corresponding to latitudes 48°N (seed origin), 52°N, 55°N, and 58°N, and two levels of soil moisture (60%–75% and 13%–20% of field capacity) for one growing season. Seedling growth, leaf size, specific leaf area, biomass allocation, and xylem resistance to cavitation (water potentials for 20%, 50%, and 80% loss of hydraulic conductivity) were assessed. The seedlings under the longest photoperiod regime (58°N latitude) had greatest height and biomass but smallest specific leaf area. Under the elevated [CO2], however, the longest photoperiod regime significantly reduced xylem resistance to drought-induced cavitation compared with the photoperiod corresponding to 48°N. These results suggest that when migrating to higher latitudes, trembling aspen may grow faster but could become less resistant to drought and more prone to hydraulic failure during a drought spell.
A structure-function analysis of Drosophila Tolloid

9 Declaration . 10 Copyright statement . 11 Acknowledgements . 12 Chapter 1 : Introduction . 13 1.1 The Tolloid metalloproteinase family . 13 1.2 Tolloid metalloproteinase roles . 15 1.3 Tolloid function in ECM formation . 18 1.3. 1 Collagens . 18 1.3. 2 Non-collagenous matrix proteins . 18 1.4 Tolloid function in growth factor regulation . 19 1.4. 1 Transforming growth factor ligands . 19 1.4. 2 Insulin-like growth factors . 20 1.4. 3 Bone morphogenetic proteins . 20 1.5 Using Drosophila embryonic DV patterning as a model to study Tolloid function. 21 1.5. 1 Dorsal-ventral patterning in Drosophila . 21 1.5. 2 The core Dpp signalling pathway. 24 1.6 Extracellular modulators in BMP gradient formation . 26 1.6. 1 Short Gastrulation (Sog) . 26 1.6. 2 Tolloid (Tld) . 28 1.6. 3 Twisted Gastrulation (Tsg) . 29 1.7 Step-wise gradient formation by extracellular modulators . 29 1.8 Extracellular BMP gradient formation in Drosophila wing vein patterning . 31 1.9 Interplay between the extracellular matrix and BMP gradient formation . 33 1.10 BMP regulation during DV axis patterning in Vertebrates . 36 1.10. 1 Chordin . 36 1.10. 2 Vertebrate Tsg . 37
Andrographolide alleviates Parkinsonism in MPTP‐PD mice via targeting mitochondrial fission mediated by dynamin‐related protein 1

Accumulating evidence indicates that mitochondrial dynamics play an important role in the progressive deterioration of dopaminergic neurons. Andrographolide has been found to exert neuroprotective effects in several models of neurological diseases. However, the mechanism of how andrographolide protects neurons in Parkinson's disease (PD) remains not fully understood.
Value of Sinogram-Affirmed Iterative Reconstruction for Evaluating Coronary Calcified Plaques

Background: Coronary computed tomography angiography (cCTA) technology as a kind of non-traumatic examination has been widely used in clinical practice. There are major issues that need to be considered. One is how to obtain high quality images and at the same time effectively reduce the radiation dose. The second is coronary artery calcified plaque artifacts that seriously affect the depiction of plaque morphology and luminal stenosis. In case of dose reduction, these artifacts are more outstanding. Objectives: This study determined the value of sinogram-affirmed iterative reconstruction (SAFIRE) technology to assess coronary-calcified plaques. This value was compared with filtered back-projection (FBP) reconstruction. Patients and Methods: Sixty-three cases with calcified plaques diagnosed via coronary CT examination were selected. The mean CT-number, noise, signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), number of calcium plaques, edges, lumen situation, and the subjective image quality ratings of the cases using FBP and SAFIRE1–SAFIRE5 (six groups) were analyzed and compared. Results: The subjective ratings of image quality using SAFIRE1–SAFIRE5 reconstructions were significantly higher than those using FBP, with SAFIRE3 achieving the highest rating. Compared with FBP reconstruction, the differences in noise, SNR, and CNR using SAFIRE1-SAFIRE5 were statistically significant (P 0.05). Conclusion: SAFIRE reconstruction provided better coronary image quality and displayed the number, morphology, and surrounding lumen of calcium plaques more accurately than traditional FBP reconstruction, with SAFIRE3 achieving the best results.