Miracle-dz/UniFAR-FTUs huggingface dataset:
Competencia digital docente en Prácticum desde la autoevaluación

Se atiende a la competencia digital docente en el Prácticum de la Facultad de Educación de la Universidad de Murcia planteando tres objetivos: explorar el uso del aula virtual en el Prácticum, identificar las herramientas más utilizadas y analizar el impacto en la actividad. El proceso docente, de las asignaturas de Prácticum de los grados de Educación Infantil, Primaria, Educación Social y Pedagogía, actúa como sujeto muestral (n = 5) con 36 estudiantes. Se utiliza un enfoque cualitativo descriptivo con informes de autoevaluación ad hoc de la profesora. Los resultados indican que el tiempo dentro del aula virtual no alcanza en docencia, organización, evaluación, planificación y gestión el 40%. La herramienta más utilizada es “Mensajes Privados” con uso de aplicaciones externas al entorno virtual institucional. El porcentaje de días de uso con respecto al total trabajados es bajo. Se precisa de tutoría virtual incidiendo en la competencia digital docente.
Epigenetic characterization of the H19/IGF2 locus in calf clones placenta

ABSTRACT: Somatic Cell Nuclear Transfer (SCNT-Cloning) is a promising technique in many areas and is based on genetically identical individuals. However, its efficiency is low. Studies suggest that the leading cause is inadequate epigenetic reprogramming. This study aimed to characterize the methylation pattern of the exon 10 regions of the IGF2 gene and the Imprinting Control Region (ICR) of the H19 gene in the placenta of cloned calves. For this study, female and male cloned calves presenting different phenotypes were used. Genomic DNA from these animals’ placenta was isolated, then treated with sodium bisulfite and amplified to the ICR/H19 and IGF2 loci. PCR products were cloned into competent bacteria and finally sequenced. A significant difference was found between controls and clones with healthy phenotypes for the ICR/H19 region. In this region, controls showed a hemimethylated pattern, as predicted in the literature due to this region has an imprinted control, while clones were showed less methylated. For the IGF2, no significant differences were found between controls and clones. These results suggest that different genomic regions in the genome may be independently reprogrammed and that failures in reprogramming the DNA methylation patterns of imprinted genes may be one of the causes of the low efficiency of SCNT.
CABBAGE KALE – A NEW FUNCTIONAL FOOD PRODUCT: GROWING CONDITIONS AND REGULATION OF THE PHYTOCHEMICAL COMPOSITION

The purpose of this work was to evaluate the specific effects of natural growth hormones (gibberellic acid, 10-6М and brassinolide, 10-6М) and commercial growth bio-stimulator (Novosil, 5 g/ha) on the enzymatic and non-enzymatic antioxidants activities of cabbage kale plants, under different growing conditions. The treatment of growth regulators was imposed by soaking cabbage kale plant seed in various growth regulators for 24 hours. The results were recorded at 85, 115 and 145 days after planting, at +80С, 00С and -60С temperature, respectively. Among the studied growth regulators, terpenoidal compounds of Novosil and gibberellic acid improved the antioxidant activity, activating enzymatic antioxidant systems and increasing the content of carotenoids and proline in leaves of cabbage kale. The steroidal compound of brassinolide raises the content of phenolic and flavonoids compounds which increased antioxidant activities. The results obtained from the study indicated that the use of growth regulars increases the synthesis of protective compounds, which developed resistance of cabbage kale plants against adverse environmental factors and improve the biomedical properties of cabbage kale. Further, the results of the study also suggested that freezing temperatures (-60С) enhanced the protective effect of growth regulators against lipid peroxidation and their antioxidant activity.
DIFFERENT TECHNIQUES OF EXTRACTION AND ANTIVIRAL ACTIVITIES OF QUERCETIN AGAINST VIRUSES SUCH AS CORONAVIRUS, INFLUENZA VIRUS, EBOLA VIRUS, ZIKA VIRUS, AND OTHER VIRUSES

Quercetin (QCT) is a flavonoid, existed as a pigment in various plants. It is a bioactive compound that has a favorable effect on human health. Our work concerns the selection of different rapid techniques for QCT extraction, published on the different scientific databases, as ultrasound-assisted enzymatic extraction process, supercritical CO2 extraction, microwave-assisted extraction and consists of researching the antiviral activity of QCT and derivatives against viruses such as coronaviruses, influenza virus, human respiratory syncytial virus, Epstein-Barr virus gastric tumor, anti-hepatitis B virus, Zika virus, and Ebola. Following this research, we found that this molecule has a broad-spectrum antiviral activity. Is it effective in controlling this new form of coronavirus, this devastating virus, which currently affects thousands of people?
ASSESSMENT OF PROBIOTIC PROPERTIES OF THE STRAIN LACTOBACILLUS PARACASEI D6 AND ITS ANTICANDIDAL ACTIVITIES

Objective: The objective of the study was to determine the probiotic properties of a strain Lactobacillus paracasei D6 (GenBank Accession No. KJ867173) and to assess its antifungal activities against certain Candida strains. Methods: Antifungal activities of Lactobacillus paracasei D6 was tested against Candida tropicalis BSS7 and C. albicans MTCC 3017. The cell-free extract was tested against the formation of biofilm and germ tube of the aforementioned Candida strains. Different probiotic activities such as tolerance to simulated gastrointestinal fluid, adhesion to hydrocarbons, and Caco-2 cell line were evaluated for the strain Lactobacillus paracasei D6. Inhibition of Candida strains to Caco-2 cell line was also tested. The strains were identified using gene sequencing followed by phylogenetic tree construction. Results: The probiotic properties of Lactobacillus paracasei D6 were found to be statistically comparable with a standard Lactobacillus plantarum MTCC 1407. The biofilm and germ tube formation patterns of the food spoilage isolate C. tropicalis BSS7 were found to be similar to the strain C. albicans MTCC 3017. Cell-free extract of Lactobacillus paracasei D6 exhibited minimum biofilm inhibitory concentration of 0.438 mg/ml against C. tropicalis BSS7, which was found to be sufficient to inhibit its germ tube formation. The adhesion of C. tropicalis BSS7 to the epithelial Caco-2 cell line was also significantly reduced by the antifungal metabolites. Conclusion: This work gives insight on possible virulent nature of the food isolate C. tropicalis BSS7. Exometabolites produced by L. paracasei D6 were able to inhibit growth, biofilm and germ tube formation of both the strains. With this work, the authors envisage the beginning of an alternative strategy for anticandidal therapy.
The Birth of a New Field: Memristive Sensors. A Review

Over the last 11 years, the field of Memristive Sensors abruptly emerged in literature. The present review paper has the aim to acknowledge such an exciting new field of research as well as to critically review its most significant outcomes. As usual in science and technology, only the concept of a new sensor has been drawn and simulated in some cases, while real demonstrators have been designed, fabricated, and demonstrated in several other cases. Moreover, several new sensing methods have been proposed, all based on memristive conductivity. Again, the new sensing principle has been shown just once in some cases, while several different sensors have been demonstrated by applying a single consolidated method in other cases. Therefore, ultimate aim of this paper is to deeply and exhaustive review the most important steps in such a new field by entering the significant details about the several kinds of sensor, while critically commenting on the key aspects of the proposed ideas and applications. The most important milestones are outlined as appeared along the course of the last decade, by also showing the temporal perspective of this totally new recently emerged field.
Bacterial diversity and predicted enzymatic function in a multipurpose surface water system – from wastewater effluent discharges to drinking water production

Background Rivers and lakes are used for multiple purposes such as for drinking water (DW) production, recreation, and as recipients of wastewater from various sources. The deterioration of surface water quality with wastewater is well-known, but less is known about the bacterial community dynamics in the affected surface waters. Understanding the bacterial community characteristics —from the source of contamination, through the watershed to the DW production process—may help safeguard human health and the environment. Results The spatial and seasonal dynamics of bacterial communities, their predicted functions, and potential health-related bacterial (PHRB) reads within the Kokemäenjoki River watershed in southwest Finland were analyzed with the 16S rRNA-gene amplicon sequencing method. Water samples were collected from various sampling points of the watershed, from its major pollution sources (sewage influent and effluent, industrial effluent, mine runoff) and different stages of the DW treatment process (pre-treatment, groundwater observation well, DW production well) by using the river water as raw water with an artificial groundwater recharge (AGR). The beta-diversity analysis revealed that bacterial communities were highly varied among sample groups (R = 0.92, p < 0.001, ANOSIM). The species richness and evenness indices were highest in surface water (Chao1; 920 ± 10) among sample groups and gradually decreased during the DW treatment process (DW production well; Chao1: 320 ± 20). Although the phylum Proteobacteria was omnipresent, its relative abundance was higher in sewage and industrial effluents (66–80%) than in surface water (55%). Phyla Firmicutes and Fusobacteria were only detected in sewage samples. Actinobacteria was more abundant in the surface water (≥13%) than in other groups (≤3%). Acidobacteria was more abundant in the DW treatment process (≥13%) than in others (≤2%). In total, the share of PHRB reads was higher in sewage and surface water than in the DW treatment samples. The seasonal effect in bacterial communities was observed only on surface water samples, with the lowest diversity during summer. Conclusions The low bacterial diversity and absence of PHRB read in the DW samples indicate AGR can produce biologically stable and microbiologically safe drinking water. Furthermore, the significantly different bacterial communities at the pollution sources compared to surface water and DW samples highlight the importance of effective wastewater treatment for protecting the environment and human health.
Understanding Autonomous Driving as Institutional Activity: Opening New Ways to React to Discriminatory Concerns in Autonomous Driving

We will elaborate on why it is appropriate to be concerned about structural discrimination induced by future autonomous vehicles—not only in the context of crash-optimization. In order to understand discrimination effects and potential strategies to reduce them, a novel conceptual perspective on how to frame autonomous driving is introduced. According to it, autonomous driving is considered an institutional activity. This perspective enables us to connect to theories about institutional justice—and open new ways to react to discriminatory concerns. To illustrate this direction, we sketch how to apply institutional justice to an autonomous highway driving system.
Negative Impact of Obesity on Dialysis Efficiency: A Prospective Cohort on Lebanese CKD Patients

Background: Obesity is considered as a risk factor in chronic kidney disease (CKD), as it may worsen renal complications and progress to advanced stages of the disease. Aims: To investigate whether the efficacy of dialysis is affected in obese CKD patients. Methods: we followed the main biological factors in dialysis patients for six months. Patients were grouped based on their sexes and body mass indexes (obese, or normal-weighted) and blood samples measurements were performed before and after dialysis. Results: The elimination of creatinine and urea by dialysis is more significant in normal-weighted than obese women. Elimination of potassium and sodium have tendency to be also more effective in normal-weighted women. No significant differences were found between normal-weighted and obese men regarding creatinine, urea, potassium, and sodium elimination. However, parathyroid hormone levels were found to be higher in obese subjects of both sexes. Conclusion: Together these findings suggest that obesity affect dialysis efficacy. phosphate, and calcium levels for 6 months, we show a decrease in the effectiveness of dialysis in obese patients.
Computer-Aided Detection (CAD) for COVID-19 based on Chest X-Ray Images using Convolutional Neural Network

Covid-19 has spread throughout the world and has been declared as a pandemic by the World Health Organization (WHO). The disease was discovered by the end of 2019 in Wuhan- China. The number of deaths continues to surge sharply and spread to many countries. Covid-19 has sent billions of people on earth into lock-down when health services struggle to cope. A swift and reliable Covid-19 diagnosis system is needed, to direct the patient to the appropriate treatment and prevent the disease dissemination. During this time, we are familiar with rapid tests and Real-Time Polymerase Chain Reaction (RT-PCR) as the procedure of Covid-19 detection. Both of these procedures tend to be impractical and require specialized laboratories that are arranged in such away. It can also take several hours to wait for the amplification process until the results are known. In this study, we introduce a Covid-19 detection system based on Chest C-Ray images using Convolutional Neural Network (CNN). The dataset consists of 1000 images, 500 images each for positive Covid-19, and Pneumonia. The CNN model that was designed consisted of three hidden layers, a fully connected layer with sigmoid activation. The evaluation was conducted to determine the performance of the proposed model using matrices of precision, recall, F1, and accuracy. The experimental results show that the proposed method provides precision, recall, F1 was 1 and 100% accuracy, respectively. This research is expected to be tested in field validation, to help the medical authorities for clinical diagnosis.
Antimicrobial and In Vivo Antifungal Potentials of Crude and Degummed Citrullus lanatus Seed Oil

Citrullus lanatus (watermelon) seed oil is one of the neglected and underutilized seed oils in Nigeria. This study was aimed at evaluating the therapeutic efficacy of crude and degummed Citrullus lanatus seed oil (CLSO) in the treatment of experimental oral candidiasis induced by Candida albicans in immunosuppressed female albino rats. In vivo antifungal activity of the extracts was analyzed by microbiological and histopathological analyses, along with renal function tests at days 7 and 14 post infection treatments. The observations in the test animal groups were compared with that of control group treated with fluconazole. Treatments with CLSO resulted in significant body weight loss. Also, there was a significant reduction in the kidney fungal burden (cfu/ml/g) of rats treated with CLSO after fourteen days post infection treatment, compared to group 3 (untreated control) rats. Histologically, group 3 (untreated) showed multifocal aggregation and widespread distribution of fungal blastospores appearing singly or in small clusters within the renal parenchyma when compared with CLSO-treated groups, which had minimal fungal blastospores that appeared singly in the renal tissues. Thus, histological data were corroborated with microbiological tests for crude and degummed CLSO, but not for the fluconazole-treated group which showed multifocal and widespread distribution of blastospores at day 14. Furthermore, within the CLSO treatment group, a significant increase in the (plasma) levels of creatinine was observed, while no significant difference in blood urea values was recorded after day 14 post infection study. Histopathological alterations in the kidney was restored to normal. CLSO could be considered as a potential antifungal agent especially the degummed CLSO and could also be employed in the management of weight loss.
Analysis of Merchandise Inventory Accounting System at Bhuana Utama Department Stores

The purpose of this study is to analyse the merchandise inventory accounting system at Bhuana Utama department store. Bhuana Utama department store is a trading business unit owned by Village-Owned Enterprises (Badan Usaha Milik Desa—BUMDes) Panji. The merchandise inventory accounting system that has been implemented by the management of Bhuana Utama department store is still manual, so it often produces unreliable and untimely information. This research is a case study research. Data collection was carried out through a process of observation, interviews, and documentation study. The results showed that the implementation of the merchandise inventory accounting system at Bhuana Utama department store had several weaknesses. First, there are concurrent duties in the cash and the accounting functions. Second, the Bhuana Utama department store does not yet have an accounting function separated from the BUMDes accounting function. Third, the employees assigned to the Bhuana Utama department store do not yet have adequate knowledge and skills on accounting, both manually and computerized.
Active Edge Sites Engineering in Nickel Cobalt Selenide Solid Solutions for Highly Efficient Hydrogen Evolution

An effective multifaceted strategy is demonstrated to increase active edge site concentration in Ni0.33Co0. 67Se2 solid solutions prepared by in situ selenization process of nickel cobalt precursor. The simultaneous control of surface, phase, and morphology result in as‐prepared ternary solid solution with extremely high electrochemically active surface area (Cdl = 197 mF cm−2), suggesting significant exposure of active sites in this ternary compound. Coupled with metallic‐like electrical conductivity and lower free energy for atomic hydrogen adsorption in Ni0.33Co0. 67Se2, identified by temperature‐dependent conductivities and density functional theory calculations, the authors have achieved unprecedented fast hydrogen evolution kinetics, approaching that of Pt. Specifically, the Ni0.33Co0. 67Se2 solid solutions show a low overpotential of 65 mV at −10 mV cm−2, with onset potential of mere 18 mV, an impressive small Tafel slope of 35 mV dec−1, and a large exchange current density of 184 µA cm−2 in acidic electrolyte. Further, it is shown that the as‐prepared Ni0.33Co0. 67Se2 solid solution not only works very well in acidic electrolyte but also delivers exceptional hydrogen evolution reaction (HER) performance in alkaline media. The outstanding HER performance makes this solid solution a promising candidate for mass hydrogen production.
NLRP3 Inflammasome Promotes the Progression of Acute Myeloid Leukemia via IL-1β Pathway

NLRP3 inflammasome has been reported to be associated with the pathogenesis of multiple solid tumors. However, the role of NLRP3 inflammasome in acute myeloid leukemia (AML) remains unclear. We showed that NLRP3 inflammasome is over-expressed and highly activated in AML bone marrow leukemia cells, which is correlated with poor prognosis. The activation of NLRP3 inflammasome in AML cells promotes leukemia cells proliferation, inhibits apoptosis and increases resistance to chemotherapy, while inactivation of NLRP3 by caspase-1 or NF-κB inhibitor shows leukemia-suppressing effects. Bayesian networks analysis and cell co-culture tests further suggest that NLRP3 inflammasome acts through IL-1β but not IL-18 in AML. Knocking down endogenous IL-1β or anti-IL-1β antibody inhibits leukemia cells whereas IL-1β cytokine enhances leukemia proliferation. In AML murine model, up-regulation of NLRP3 increases the leukemia burden in bone marrow, spleen and liver, and shortens the survival time; furthermore, knocking out NLRP3 inhibits leukemia progression. Collectively, all these evidences demonstrate that NLRP3 inflammasome promotes AML progression in an IL-1β dependent manner, and targeting NLRP3 inflammasome may provide a novel therapeutic option for AML.
Influências Regionais na Mobilidade de Turistas e Residentes Usando Dados de Mídia Social

As redes sociais baseadas em localização (LBSNs) disponibilizam uma nova variedade de possibilidades para obtenção de dados em larga escala, especialmente com o significativo aumento de usuários de mídias sociais. Nesse sentido, a proposta do trabalho atual é explorar dados de LBSNs para estudar as influências regionais no comportamento de turistas e residentes no âmbito de mobilidade, levando em consideração não somente as coordenadas geográficas, mas também o tipo de local visitado. Os resultados apresentados sugerem que residentes de uma mesma região geográfica tendem a ter hábitos mais similares entre si. Observou-se também que o comportamento entre turistas e residentes é diferente. Além disso, os experimentos indicaram que o país de origem de residência do usuário pode influenciar em suas escolhas enquanto turista em outros países. As análises realizadas permitiram observar também a existência de dois grupos distintos de turistas, sendo um deles mais previsível em termos de coordenadas geográficas e tipo de local visitado. A possibilidade de explorar as características culturais de cada nacionalidade em diferentes destinos mostra-se um caminho promissor para melhorar sistemas de recomendação de locais e serviços oferecidos para grupos de turistas distintos.
Keputusan Pembelian Konsumen pada Yogya Grand Majalengka Dilihat dari Perspektif Iklan dan Komunikasi Word Of Mouth

Penelitian ini bertujuan untuk mengetahui atau menganalisa pengaruh iklan dan word of mouth (WOM) terhadap keputusan pembelian konsumen di Yogya Grand Majalengka. Metode yang digunakan adalah deskriptif kuantitatif dengan pendekatan eksplanatori survey. Survey dilakukan dengan menyebarkan kuesioner yang dibuat melalui google form dan didapat respon sebanyak 167 feedback untuk dianalisa. Data dianalisa melalui uji asumsi klasik, uji hipotesis, uji regresi linear berganda, dan koefisien determinasi menggunakan aplikasi SPSS. Iklan berpengaruh negative terhadap keputusan pembelian, sedangkan WOM baik secara parsial maupun simultan bersama iklan berpengaruh positif terhadap keputusan pembelian. Variabel bebas tidak dapat menyediakan penjelasan secara menyeluruh dalam menjawab permasalahan keputusan pembelian konsumen di Yogya Grand Majalengka. Temuan penelitian dapat digunakan sebagai informasi bagi pihak Yogya Grand Majalengka dalam menentukan strategi pemasaran dikemudian hari dan sebagai bukti empiris yang mungkin berguna bagi penelitian selanjutnya pada topik manajemen pemasaran.