Miracle-dz/UniFAR-FTUs huggingface dataset:
Advance Directives on Hospital Admission: A Survey of Patient Attitudes

A survey of 200 outpatients using an anonymous, self-administered questionnaire revealed that 18% had already completed an advance directive. Only 5% had received information concerning advance directives from their physicians. Eighty-seven percent stated they would not be offended if, on admission to the hospital, they were to be asked whether they had completed a living will. Eighty-eight percent would view such a policy as showing evidence of positive concern by the hospital on their behalf. Only 2% consistently gave a negative response to a proposed policy of hospitals providing information concerning advance directives on admission, and a similar percentage was totally opposed to their use. Of those who had completed an advance directive, 50% had secured the only copy in a safety deposit box. This information, as well as the Patient Self-Determination Act of 1990, which will as of December 1, 1991 require hospitals to provide patients with information about advance directives, creates a new framework for the more efficient use of these important but underused documents.
Effects of pre-partum milking of dairy cows on calcium metabolism at start of milking and at calving.

This experiment studied the effect of pre-partal milk removal on calcium metabolism at start of milking and at calving. Nine cows of the Swedish Red breed were milked for 1-7 days pre-partum. The average milk yield at the first milking was 4.8 l, and the average yield the last day prior to calving was 13.4 l. Five cows were used as control cows and were only milked post-partum. Samples of plasma and urine were taken to determine the effect of pre-partum milking and calving on levels of calcium, magnesium, parathyroid hormone and plasma C-terminal crosslinked telopeptide of type 1-collagen (CTx), used as a marker of bone resorption. Pre-partum milking resulted in a decrease in plasma calcium that was evident 2 days after the first milking. Parathyroid hormone increased at the same time, and CTx started to increase from 24 h after the first milking. There were no effects on plasma magnesium or urinary output of calcium or magnesium. The first week after calving, there were no differences between pre-partum milked cows and control cows in plasma or urine variables, or in milk yield. In conclusion, pre-partum milking activated the calcium-restoring mechanisms but did not improve calcium status at calving.
Changes in regional variation of Medicare home health care utilization and service mix for patients undergoing major orthopedic procedures in response to changes in reimbursement policy.

BACKGROUND Significant variation in regional utilization of home health (HH) services has been documented. Under Medicare's Home Health Interim and Prospective Payment Systems, reimbursement policies designed to curb expenditure growth and reduce regional variation were instituted. OBJECTIVE To examine the impact of Medicare reimbursement policy on regional variation in HH care utilization and type of HH services delivered. RESEARCH DESIGN We postulated that the reimbursement changes would reduce regional variation in HH services and that HH agencies would respond by reducing less skilled HH aide visits disproportionately compared with physical therapy or nursing visits. An interrupted time-series analysis was conducted to examine regional variation in the month-to-month probability of HH selection, and the number of and type of visits among HH users. SUBJECTS A 100 percent sample of all Medicare recipients undergoing either elective joint replacement (1.6 million hospital discharges) or surgical management of hip fracture (1.2 million hospital discharges) between January 1996 and December 2001 was selected. RESULTS Before the reimbursement changes, there was great variability in the probability of HH selection and the number of HH visits provided across regions. In response to the reimbursement changes, though there was little change in the variation of probability of HH utilization, there were marked reductions in the number and variation of HH visits, with greatest reductions in regions with highest baseline utilization. HH aide visits were the source of the baseline variation and accounted for the majority of the reductions in utilization after implementation. CONCLUSIONS The HH interim and prospective payment policies were effective in reducing regional variation in HH utilization.
[Role of mitochondrial lesion in pathogenesis of sporadic rett syndrome].

OBJECTIVE To investigate the role of mitochondrial lesion in children with Rett syndrome (RTT). METHODS The platelets from 6 cases with Rett syndrome and 9 normal controls were fused with mitochondrial DNA-lacking rho degrees cell by polyethylene glycol-mediated fusion technique. The oxygen free radical was evaluated by the polarography with substrates of vitamine C and TMPD (N, N, N', N'-tetramethyl-p-phenlene diamine). The rate of apoptosis was determinated by flow cytometry. The apoptosis was observed by electronic microscope and TUNEL method. The t test between groups was adopted in study. RESULTS In RTT patients, the anticyano-respiration significantly increased by 23% (t = 4.76, P < 0.01). After 6-hour coincubation with 100 micromol/L H(2)O(2), the rate of apoptosis is (3.6 +/- 1.1) % in normal control, and (9.9 +/- 2.7) % in RTT group with significant difference (t = 6.30, P < 0.01), the existence of apoptosis was confirmed by electronic microscope and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end-labeling (TUNEL). CONCLUSION After mitochondrial DNA transfer, the oxygen free radical increased in mitochondrial respiratory chain in cybrids of RTT children. RTT cybrid cell lines demonstrated an increased sensitivity to H(2)O(2)-induced apoptotic cell death as compared to control cybrids. The mitochondrial lesion might play a role in the pathogenesis of Rett syndrome.
Expression of matrix-degrading enzymes in pulmonary vascular remodeling in the rat

Thakker-Varia, Smita, Carol A. Tozzi, George J. Poiani, Joanne P. Babiarz, Linda Tatem, Frank J. Wilson, and David J. Riley. Expression of matrix-degrading enzymes in pulmonary vascular remodeling in the rat. Am. J. Physiol. 275 (Lung Cell. Mol. Physiol. 19): L398–L406, 1998. —Exposure of rats to hypoxia causes pulmonary arterial remodeling, which is partly reversible after return to air. We hypothesized that degradation of excess collagen in remodeled pulmonary arteries in the posthypoxic period is mediated by endogenous matrix metalloproteinases (MMPs). Total proteolytic, collagenolytic, and gelatinolytic activities, levels of stromelysin-1 and tissue inhibitor of metalloprotease-1 (TIMP-1), and immunolocalization of stromelysin-1 in main pulmonary arteries were determined after exposure of rats to 10% O2 for 10 days followed by normoxia. We observed transient increases in total proteolytic, collagenolytic, and gelatinolytic activities and expression of ,72-, 68-, and 60-kDa gelatinases by zymography within 3 days of cessation of hypoxic exposure. The level of TIMP-1 increased as the stromelysin-1 level increased. Immunoreactive stromelysin-1 was localized predominantly in the luminal region of normal and hypertensive pulmonary arteries. These results are consistent with the notion that endogenous MMPs may mediate the breakdown of excess collagen in remodeled pulmonary arteries during the early posthypoxic period.
A novel gene expressed in rat ameloblasts codes for proteins with cell binding domains

Two variants of an mRNA sequence are identified that are expressed at high levels in rat ameloblasts during the formation of the enamel matrix. The sequences contain open reading frames for 407 and 324 amino acid residues, respectively. The encoded proteins, which we call amelins, are rich in proline, glycine, leucine, and alanine residues and contain the peptide domain DGEA, an integrin recognition sequence. The sequences coding for the C‐terminal 305 amino acid residues, the 3′ nontranslated part, and a microsatellite repeat at the nontranslated 5′ region are identical in both mRNA variants. The remaining 5′ regions contain 338 nucleotides unique to the long variant, 54 common nucleotides, and 46 nucleotides present only in the short variant. Eleven nucleotides have the potential to code for 5 amino acids of both proteins in different reading frames. The reading frame of the longer variant includes codons for a typical N‐terminal signal peptide. The amelins are likely to be constituents of the enamel matrix and the only proteins that have so far been implicated in binding interactions between the ameloblast surface and its extracellular matrix. (J Bone Miner Res 1996;11:883‐891)
Homocysteic acid induces intraneuronal accumulation of neurotoxic Aβ42: Implications for the pathogenesis of Alzheimer's disease

The causes of neuronal dysfunction and degeneration in Alzheimer's disease (AD) are not fully understood, but increased production of neurotoxic forms of amyloid β‐peptide‐42 (Aβ42) seems of major importance. Large extracellular deposits of aggregated Aβ42 (plaques) is a diagnostic feature of AD, but Aβ42 may be particularly cytotoxic when it accumulates inside neurons. The factors that may promote the intracellular accumulation of Aβ42 in AD are unknown, but recent findings suggest that individuals with elevated homocysteine levels are at increased risk for AD. We show that homocysteic acid (HA), an oxidized metabolite of homocysteine, induces intraneuronal accumulation of a Aβ42 that is associated with cytotoxicity. The neurotoxicity of HA can be attenuated by an inhibitor of γ‐secretase, the enzyme activity that generates Aβ42, suggesting a key role for intracellular Aβ42 accumulation in the neurotoxic action of HA. Concentrations of HA in cerebrospinal fluid (CSF) were similar in AD and control subjects. CSF homocysteine levels were elevated significantly in AD patients, however, and homocysteine exacerbated HA‐induced neurotoxicity, suggesting a role for HA in the pathogenic action of elevated homocysteine levels in AD. These findings suggest that the intracellular accumulation of Aβ42 plays a role in the neurotoxic action of HA, and suggest a potential therapeutic benefit of agents that modify the production and neurotoxic actions of HA and homocysteine. © 2005 Wiley‐Liss, Inc.
The Positivity Bias Phenomenon in Face Perception Given Different Information on Ability

The negativity bias has been shown in many fields, including in face processing. We assume that this bias stems from the potential threat inlayed in the stimuli (e.g., negative moral behaviors) in previous studies. In the present study, we conducted one behavioral and one event-related potentials (ERPs) experiments to test whether the positivity bias rather than negativity bias will arise when participants process information whose negative aspect involves no threat, i.e., the ability information. In both experiments, participants first completed a valence rating (negative-to-positive) of neutral facial expressions. Further, in the learning period, participants associated the neutral faces with high-ability, low-ability, or control sentences. Finally, participants rated these facial expressions again. Results of the behavioral experiment showed that compared with pre-learning, the expressions of the faces associated with high ability sentences were classified as more positive in the post-learning expression rating task, and the faces associated with low ability sentences were evaluated as more negative. Meanwhile, the change in the high-ability group was greater than that of the low-ability group. The ERP data showed that the faces associated with high-ability sentences elicited a larger early posterior negativity, an ERP component considered to reflect early sensory processing of the emotional stimuli, than the faces associated with control sentences. However, no such effect was found in faces associated with low-ability sentences. To conclude, high ability sentences exerted stronger influence on expression perception than did low ability ones. Thus, we found a positivity bias in this ability-related facial perceptual task. Our findings demonstrate an effect of valenced ability information on face perception, thereby adding to the evidence on the opinion that person-related knowledge can influence face processing. What’s more, the positivity bias in non-threatening surroundings increases scope for studies on processing bias.
Diagnosis & treatment of tuberculosis in HIV co-infected patients

Human immunodeficiency virus (HIV) associated tuberculosis (TB) remains a major global public health challenge, with an estimated 1.4 million patients worldwide. Co-infection with HIV leads to challenges in both the diagnosis and treatment of tuberculosis. Further, there has been an increase in rates of drug resistant tuberculosis, including multi-drug (MDR-TB) and extensively drug resistant TB (XDRTB), which are difficult to treat and contribute to increased mortality. Because of the poor performance of sputum smear microscopy in HIV-infected patients, newer diagnostic tests are urgently required that are not only sensitive and specific but easy to use in remote and resource-constrained settings. The treatment of co-infected patients requires antituberculosis and antiretroviral drugs to be administered concomitantly; challenges include pill burden and patient compliance, drug interactions, overlapping toxic effects, and immune reconstitution inflammatory syndrome. Also important questions about the duration and schedule of anti-TB drug regimens and timing of antiretroviral therapy remain unanswered. From a programmatic point of view, screening of all HIV-infected persons for TB and vice-versa requires good co-ordination and communication between the TB and AIDS control programmes. Linkage of co-infected patients to antiretroviral treatment centres is critical if early mortality is to be prevented. We present here an overview of existing diagnostic strategies, new tests in the pipeline and recommendations for treatment of patients with HIV-TB dual infection.
Expression profiling during ocular development identifies 2 Nlz genes with a critical role in optic fissure closure

The gene networks underlying closure of the optic fissure during vertebrate eye development are poorly understood. Here, we profile global gene expression during optic fissure closure using laser capture microdissected (LCM) tissue from the margins of the fissure. From these data, we identify a unique role for the C2H2 zinc finger proteins Nlz1 and Nlz2 in normal fissure closure. Gene knockdown of nlz1 and/or nlz2 in zebrafish leads to a failure of the optic fissure to close, a phenotype which closely resembles that seen in human uveal coloboma. We also identify misregulation of pax2 in the developing eye of morphant fish, suggesting that Nlz1 and Nlz2 act upstream of the Pax2 pathway in directing proper closure of the optic fissure.
Infectious Bursal Disease Virus Capsid Protein VP3 Interacts both with VP1, the RNA-Dependent RNA Polymerase, and with Viral Double-Stranded RNA

ABSTRACT Infectious bursal disease virus (IBDV) is a double-stranded RNA (dsRNA) virus of the Birnaviridae family. Its two genome segments are encapsidated together with multiple copies of the viral RNA-dependent RNA polymerase, VP1, in a single-shell capsid that is composed of VP2 and VP3. In this study we identified the domains responsible for the interaction between VP3 and VP1. Using the yeast two-hybrid system we found that VP1 binds to VP3 through an internal domain, while VP3 interacts with VP1 solely by its carboxy-terminal 10 amino acids. These results were confirmed by using a reverse-genetics system that allowed us to analyze the interaction of carboxy-terminally truncated VP3 molecules with VP1 in infected cells. Coimmunoprecipitations with VP1- and VP3-specific antibodies revealed that the interaction is extremely sensitive to truncation of VP3. The mere deletion of the C-terminal residue reduced coprecipitation almost completely and also fully abolished production of infectious virions. Surprisingly, these experiments additionally revealed that VP3 also binds to RNA. RNase treatments and reverse transcription-PCR analyses of the immunoprecipitates demonstrated that VP3 interacts with dsRNA of both viral genome segments. This interaction is not mediated by the carboxy-terminal domain of VP3 since C-terminal truncations of 1, 5, or 10 residues did not prevent formation of the VP3-dsRNA complexes. VP3 seems to be the key organizer of birnavirus structure, as it maintains critical interactions with all components of the viral particle: itself, VP2, VP1, and the two genomic dsRNAs.
Women and Aids in Zimbabwe: The Making of an Epidemic

As the AIDS epidemic in Africa assumes major proportions, the need to understand the social context in which heterosexual transmission occurs takes on urgent importance. In this article we explore how the intersection of traditional culture with the colonial legacy and present-day political economy has influenced family structure and sexual relations, and particularly the social position of women. Drawing on Zimbabwe's historical experience, we show how land expropriation, rural impoverishment, and the forcible introduction of male migrant labor fostered new patterns of sexual relations, characterized by multiple partners. Traditional patriarchal values reinterpreted in European law resulted in further subjugation of women as even limited rights to ownership were withdrawn. For many women, sexual relations with men, either within marriage (for the majority) or outside, become inextricably linked to economic and social survival. In this setting, all sexually transmitted diseases became rampant, including genital ulcer, which facilitates transmission of the human immunodeficiency virus (HIV). Intervention programs to halt the spread of AIDS need to take into the account the epidemic's historical roots and social nature. For example, efforts to reduce risk of HIV transmission should seek to expand women's limited options, both technically (e.g., by providing alternatives to condoms) and socially (e.g., by promoting employment).
Surgical Management Of Varicose Veins: Meta-analysis

Open surgery remains the gold standard by which endovascular treatment of superficial chronic venous insufficiency is measured. This meta-analysis of randomized controlled trials reviews the current evidence base, comparing open and endovascular treatment of varicose veins. Systematic review of studies reporting duplex scan follow-up after open surgical, laser (endovenous laser therapy [EVLT]), or radiofrequency (VNUS Closure device, VNUS Medical Technologies, San Jose, CA) treatment of refluxing great saphenous veins was completed. Primary outcome measures were occlusion and complication rates and time taken to resume work. No significant difference in recurrence rates at 3 months between open surgery and EVLT (RR 2.19, 95% CI 0.99–4.85, p = .05) or VNUS device (RR 7.57; 95% CI 0.42–136.02) were found. Return to work is significantly faster following VNUS (by 8.24 days; 95% CI 10.50–5.97) or EVLT (by 5.02 days; 95% CI 6.52–3.52). Endovascular treatment of varicose veins is safe and effective and offers the significant advantage of rapid recovery.
A silicone‐based controlled‐release device for accelerated proteolytic debridement of wounds

A new device for rapid enzymatic debridement of cutaneous wounds has been developed using a controlled‐release, silicone‐based, dried emulsion. A dehydrated serine protease of the subtilisin family, previously untested for wound debridement, was incorporated into the emulsion. This device exhibited excellent storage stability. Moisture from the wound triggered an even, reproducible, and complete release of the enzyme within the first 8 hours. The device maintains a moist wound environment that allows the enzyme to achieve nearly complete digestion of the hardened eschar of full‐thickness burns in a porcine model after an exposure period of 24 hours. Debridement was faster than in untreated wounds or wounds treated with a currently available enzyme ointment. Following rapid enzymatic debridement, healing appeared to progress normally, with no histological evidence of damage to adjacent healthy tissue.
Polygenic risk, rapid childhood growth, and the development of obesity: evidence from a 4-decade longitudinal study.

OBJECTIVE To test how genomic loci identified in genome-wide association studies influence the development of obesity. DESIGN A 38-year prospective longitudinal study of a representative birth cohort. SETTING The Dunedin Multidisciplinary Health and Development Study, Dunedin, New Zealand. PARTICIPANTS One thousand thirty-seven male and female study members. MAIN EXPOSURES We assessed genetic risk with a multilocus genetic risk score. The genetic risk score was composed of single-nucleotide polymorphisms identified in genome-wide association studies of obesity-related phenotypes. We assessed family history from parent body mass index data collected when study members were 11 years of age. MAIN OUTCOME MEASURES Body mass index growth curves, developmental phenotypes of obesity, and adult obesity outcomes were defined from anthropometric assessments at birth and at 12 subsequent in-person interviews through 38 years of age. RESULTS Individuals with higher genetic risk scores were more likely to be chronically obese in adulthood. Genetic risk first manifested as rapid growth during early childhood. Genetic risk was unrelated to birth weight. After birth, children at higher genetic risk gained weight more rapidly and reached adiposity rebound earlier and at a higher body mass index. In turn, these developmental phenotypes predicted adult obesity, mediating about half the genetic effect on adult obesity risk. Genetic associations with growth and obesity risk were independent of family history, indicating that the genetic risk score could provide novel information to clinicians. CONCLUSIONS Genetic variation linked with obesity risk operates, in part, through accelerating growth in the early childhood years after birth. Etiological research and prevention strategies should target early childhood to address the obesity epidemic.
Do Natural Disasters Open a Window of Opportunity for Corruption?

Abstract This study explores the link between natural disasters and corruption at the local government level. We examine whether a natural disaster affects official households more favourably than non-official households. We find that natural disasters decrease nonofficial household expenditures significantly, however, they have negligible effect on official household expenditures. Meanwhile, both kinds of households experience similar reduction in incomes, and have much the same disaster coping strategies. Together, the results imply that local officials may receive unobserved monetary compensation – we define as corruption – in the aftermath of natural disasters.
Adaptive control of a class of nonlinear discrete-time systems using hybrid neural networks

In this paper, an indirect adaptive controller based on hybrid neural networks, which are composed of two-layered neural networks and radial basis function (RBF) neural networks, is derived for controlling a class of unknown nonlinear discrete-time systems. A hybrid-neural-network-based estimator is used to characterize the input-output behavior of the unknown systems. An adaptation law which adjusts the connection weights of the neural network is used to minimize the error signal which is difference between the actual response and that of the neural network. The indirect adaptive control law is generated on-line using another hybrid neural network related to the estimator, so that the plant results in a bounded tracking error with respect to a desired reference signal. It is proved that the control objective is achieved by the closed-loop system and that the system remains closed-loop stability. The effectiveness of the proposed control scheme is also demonstrated by a simulation example.