Miracle-dz/UniFAR-FTUs huggingface dataset:
Analysis of continuous glucose monitoring data from non-diabetic and diabetic children: a tale of two algorithms.

Use of the Medtronic MiniMed Continuous Glucose Monitoring System (CGMS) in non-diabetic children has revealed many low and high sensor glucose (SG) values, suggesting that the original analytical algorithm (Solutions 2.0) might be overreading glycemic excursions. A revised algorithm (Solutions 3.0) was introduced in 2001. Our aim was to compare analyses of the same sensor profiles using both programs. Twenty-five lean, non-diabetic subjects (mean age 14 +/- 4 years) underwent continuous glucose monitoring with CGMS for up to 72 h. Sensor tracings were analyzed with both algorithms and compared. Separate analyses were performed for nocturnal readings (12-6 a.m.). Mean SG values were similar (103 +/- 24 mg/dL for version 2.0 vs. 100 +/- 14 for version 3.0), but the distribution was significantly different: 13.8% of total SG were <70 mg/dL by version 2.0 versus 8.2% by version 3.0 (p < 0.001), and 7.7% of total SG were >150 mg/dL by version 2.0 versus 4.7% by version 3.0 (p = 0.02). Of nocturnal SG values, 25.8% were <70 mg/dL by version 2.0 compared with 17.9% by version 3.0, and 9.4% were >150 mg/dL by version 2.0 compared with 4.0% by version 3.0. In lean non-diabetic children, Solutions 2.0 identified significantly more hypoglycemia and hyperglycemia than Solutions 3.0. Similar analyses in 40 children with type 1 diabetes revealed no significant differences. Solutions 3.0 may be a more useful algorithm for preventing over-reading of low and high SG readings in non-diabetic children, whereas both algorithms give similar results in children with diabetes.
Differences in healthy food supply and stocking practices between small grocery stores, gas-marts, pharmacies and dollar stores

Abstract Objective Little is known about the practices for stocking and procuring healthy food in non-traditional food retailers (e.g. gas-marts, pharmacies). The present study aimed to: (i) compare availability of healthy food items across small food store types; and (ii) examine owner/manager perceptions and stocking practices for healthy food across store types. Design Descriptive analyses were conducted among corner/small grocery stores, gas-marts, pharmacies and dollar stores. Data from store inventories were used to examine availability of twelve healthy food types and an overall healthy food supply score. Interviews with managers assessed stocking practices and profitability. Setting Small stores in Minneapolis and St. Paul, MN, USA, not participating in the Special Supplemental Nutrition Program for Women, Infants, and Children. Subjects One hundred and nineteen small food retailers and seventy-one store managers. Results Availability of specific items varied across store type. Only corner/small grocery stores commonly sold fresh vegetables (63 % v. 8 % of gas-marts, 0 % of dollar stores and 23 % of pharmacies). More than half of managers stocking produce relied on cash-and-carry practices to stock fresh fruit (53 %) and vegetables (55 %), instead of direct store delivery. Most healthy foods were perceived by managers to have at least average profitability. Conclusions Interventions to improve healthy food offerings in small stores should consider the diverse environments, stocking practices and supply mechanisms of small stores, particularly non-traditional food retailers. Improvements may require technical support, customer engagement and innovative distribution practices.
Mycobacterial lung disease in cystic fibrosis: a prospective study.

BACKGROUND Patients with cystic fibrosis (CF) may be predisposed to airway infections with unusual organisms, such as mycobacteria. The aim of the study was to determine the incidence and clinical picture of mycobacterial infection in CF children. METHODS At least 2 acid-fast bacillus (AFB) smears and mycobacterial cultures were performed on a prospective basis on 682 sputum specimens from 106 patients during a 1-year period. RESULTS Thirty-three percent of the cultures were contaminated with other bacteria. Seven children had at least one sputum culture positive for one mycobacterium. Five children had only one positive AFB culture. Their clinical status and lung function remained stable during follow-up. Two teenagers with severe lung disease had several positive AFB smears and cultures for Mycobacterium chelonae and Mycobacterium abscessus. The isolation of M. chelonae and M. abscessus was associated with a clinical and functional decline. Clarithromycin treatment resulted in temporary improvement with the disappearance of the mycobacteria after 6 months of treatment. This prospective study shows an incidence of 2.3% for positive cultures. The prevalence was 6.6% for mycobacterial colonization but only 1.9% for mycobacterial lung disease in our pediatric population. CONCLUSIONS We recommend performing AFB smears and cultures in CF children with severe lung disease and/or during a lung exacerbation. In these patients persistence of M. chelonae or M. abscessus in sputum should lead to consideration of treatment with clarithromycin.
Apuntes sobre la entonacin de la ciudad de Zaragoza

En este articulo esbozamos brevemente los elementos acusticos que hemos encontrado en un analisis de dos conversaciones espontaneas grabadas a dos informantes de la ciudad de Zaragoza. Ambos hablantes fueron escogidos intencionadamente por su marcado «acento» zaragozano. Tras estudiar los datos creemos que la peculiaridad de la entonacion de esta ciudad se encuentra caracterizada por la realizacion de un contorno de F0 en forma circunfleja. Este contorno se realiza en la silaba postonica. Ademas, los rasgos prosodicos de duracion e intensidad tambien ayudan a percibir la peculiar prosodia de la zona. La silaba con este contorno tonal suele ser particularmente larga, especialmente cuando va en final de frase. Su intensidad tambien es particularmente elevada.
Biexponential diffusion attenuation in various states of brain tissue: Implications for diffusion‐weighted imaging

Diffusion‐weighted single voxel experiments conducted at b‐values up to 1 × 104 smm−2 yielded biexponential signal attenuation curves for both normal and ischemic brain. The relative fractions of the rapidly and slowly decaying components (f1, f2)are f1 = 0.80 ± 0.02, f2 = 0.17 ± 0.02 in healthy adult rat brain and f1 = 0.90 ± 0.02, f2 = 0.11 ± 0.01 in normal neonatal rat brain, whereas the corresponding values for the postmortem situation are f1 = 0.69 ± 0.02, f2 = 0.33 ± 0.02. It is demonstrated that the changes in f1 and f2 occur simultaneously to those in the extracellular and intracellular space fractions (fex, fin) during: (i) cell swelling after total circulatory arrest, and (ii) the recovery from N‐methyl‐D‐aspartate induced excitotoxic brain edema evoked by MK‐801, as measured by changes in the electrical impedance. Possible reasons for the discrepancy between the estimated magnitude components and the physiological values are presented and evaluated. Implications of the biexponential signal attenuation curves for diffusion‐weighted imaging experiments are discussed.
Preference for social contact versus environmental enrichment in male laboratory mice

Due to their aggressive nature, male mice are less frequently used than female mice in biomedical research. When aggressive males are being used, individual housing is common practice. The question arises whether this is an acceptable housing for a social species. The present study was designed to gain more insight into the nature of inter-male social contact and into the potential of a form of environmental enrichment (nesting material) to compensate for the lack of social contact. In a series of tests, we analysed whether male mice of different ages preferred to spend time (1) near a familiar cage mate versus an empty cage, or (2) near to a familiar cage mate versus direct contact with nesting material (tissues). Dwelling time in each of the test cages and sleeping sites was recorded, as was the behaviour of the test mice. Results indicated that when other conditions were similar, male mice preferred to sleep in close proximity to their familiar cage mate. Furthermore, the need to engage in active social behaviour increased with age. Tissues were used to a large extent for sleeping and sleep-related behaviour. It is concluded that single housing in order to avoid aggression between male mice is a solution with evident negative consequences for the animals. When individual housing is inevitable due to excessive aggressive behaviour, the presence of nesting material could partly compensate for the deprivation of social contact.
An Analysis of Impact Factors for Positioning Performance in WLAN Fingerprinting Systems Using Ishikawa Diagrams and a Simulation Platform

Many factors influence the positioning performance in WLAN RSSI fingerprinting systems, and summary of these factors is an important but challenging job. Moreover, impact analysis on nonalgorithm factors is significant to system application and quality control but little research has been conducted. This paper analyzes and summarizes the potential impact factors by using an Ishikawa diagram considering radio signal transmitting, propagating, receiving, and processing. A simulation platform was developed to facilitate the analysis experiment, and the paper classifies the potential factors into controllable, uncontrollable, nuisance, and held-constant factors considering simulation feasibility. It takes five nonalgorithm controllable factors including APs density, APs distribution, radio signal propagating attenuation factor, radio signal propagating noise, and RPs density into consideration and adopted the OFAT analysis method in experiment. The positioning result was achieved by using the deterministic and probabilistic algorithms, and the error was presented by RMSE and CDF. The results indicate that the high APs density, signal propagating attenuation factor, and RPs density, with the low signal propagating noise level, are favorable to better performance, while APs distribution has no particular impact pattern on the positioning error. Overall, this paper has made great potential contribution to the quality control of WLAN fingerprinting solutions.
Lung dynamic MRI deblurring using low-rank decomposition and dictionary learning.

PURPOSE Lung dynamic MRI (dMRI) has emerged to be an appealing tool to quantify lung motion for both planning and treatment guidance purposes. However, this modality can result in blurry images due to intrinsically low signal-to-noise ratio in the lung and spatial/temporal interpolation. The image blurring could adversely affect the image processing that depends on the availability of fine landmarks. The purpose of this study is to reduce dMRI blurring using image postprocessing. METHODS To enhance the image quality and exploit the spatiotemporal continuity of dMRI sequences, a low-rank decomposition and dictionary learning (LDDL) method was employed to deblur lung dMRI and enhance the conspicuity of lung blood vessels. Fifty frames of continuous 2D coronal dMRI frames using a steady state free precession sequence were obtained from five subjects including two healthy volunteer and three lung cancer patients. In LDDL, the lung dMRI was decomposed into sparse and low-rank components. Dictionary learning was employed to estimate the blurring kernel based on the whole image, low-rank or sparse component of the first image in the lung MRI sequence. Deblurring was performed on the whole image sequences using deconvolution based on the estimated blur kernel. The deblurring results were quantified using an automated blood vessel extraction method based on the classification of Hessian matrix filtered images. Accuracy of automated extraction was calculated using manual segmentation of the blood vessels as the ground truth. RESULTS In the pilot study, LDDL based on the blurring kernel estimated from the sparse component led to performance superior to the other ways of kernel estimation. LDDL consistently improved image contrast and fine feature conspicuity of the original MRI without introducing artifacts. The accuracy of automated blood vessel extraction was on average increased by 16% using manual segmentation as the ground truth. CONCLUSIONS Image blurring in dMRI images can be effectively reduced using a low-rank decomposition and dictionary learning method using kernels estimated by the sparse component.
Suppression of Survivin Expression by Short Hairpin RNA Induces Apoptosis in Human Laryngeal Carcinoma Cells

Purpose: The aim of this study was to evaluate the suppression effect of survivin shRNA on the expression of the survivin gene in the human laryngeal cancer cell line Hep-2. Procedures: 60 cases of laryngeal squamous-cell carcinoma (LSCC) and 10 cases of normal laryngeal mucosa were examined using immunohistochemistry to determine whether the expression of survivin correlated with tumorigenesis. Three plasmid vectors of short hairpin RNA (shRNA) specific for survivin were designed and generated. Western blot and real-time PCR analysis of survivin expression in Hep-2 cells was performed 48 h after transfection. The growth curve was used to determine the cell proliferation. Propidium iodide (PI) single staining was applied to detect the cell cycle. The apoptosis of the cells was analyzed by flow cytometry with the FITC-annexin-V/PI double staining and PI single staining. Results: 68.33% (41 out of 60) of tumors were positive for survivin expression and significantly associated with lymph node metastasis and advanced stage. In contrast, no expression of survivin in normal mucosa was detected. Transfection of Hep-2 cells with survivin shRNA significantly inhibited survivin expression at both the mRNA and the protein level in Hep-2 cells. Downregulation of survivin resulted in increasing the apoptosis index, but the results showed no obvious influence on cell cycle. Conclusions: This study demonstrates that survivin shRNA effectively inhibits survivin gene expression in Hep-2 cells leading to growth suppression and apoptotic induction in Hep-2 cells.
Characterization of the Pac25I restriction-modification genes isolated from the endogenous pRA2 plasmid of Pseudomonas alcaligenes NCIB 9867.

Genes for the class II Pseudomonas alcaligenes NCIB 9867 restriction-modification (R-M) system, Pac25I, have been cloned from its 33-kb endogenous plasmid, pRA2. The Pac25I endonuclease and methylase genes were found to be aligned in a head-to-tail orientation with the methylase gene preceding and overlapping the endonuclease gene by 1 bp. The deduced amino acid sequence of the Pac25I methylase revealed significant similarity with the XcyI, XmaI, Cfr9I, and SmaI methylases. High sequence similarity was displayed between the Pac25I endonuclease and the XcyI, XmaI, and Cfr9I endonucleases which cleave between the external cytosines of the recognition sequence (i.e., 5'-C CCGGG-3') and are thus perfect isoschizomers. However, no sequence similarity was detected between the Pac25I endonuclease and the SmaI endonuclease which cleaves between the internal CpG of the recognition sequence (i.e., 5'-CCCGGG-3'). Both the Pac25I methylase and endonuclease were expressed in Escherichia coli. An open reading frame encoding a protein which shows significant similarity to invertases and resolvases was located immediately upstream of the Pac25I R-M operon. In addition, a transposon designated Tn5563 was located 1531 bp downstream of the R-M genes. The location on a self-transmissible plasmid as well as the close association with genes involved in DNA mobility suggests horizontal transfer as a possible mode of distribution of this family of R-M genes in various bacteria.
Controlling Data Congestion by Efficient Backpressure Routing Algorithm in V2V Communication for Autonomous Vehicles

As Automated cars are gaining more and more interest among the public, the importance of Vehicle-to-Vehicle(V2V) communication has also increased. As the number of V2V vehicles increase there is a condition that occurs called congestion that occurs due to too many vehicles trying to communicate with each other. Thus, we require some protocols that can instruct when the vehicles can perform a V2V to avoid congestion. These congestion control protocols are important in dense vehicular networks. The traditional backpressure routing algorithm investigates every attainable way between source and goal. The strategy is effective when the system is intensely loaded but the most important issue emerges when the communication routes are moderately blocked which increases throughput. To defeat this issue, we are proposing Efficient Backpressure Routing (EBR) algorithm that enhances average throughput what’s more, exhausts a normal power. It chooses many ideal courses in view of the brief way data. When contrasted with the Traditional Backpressure Algorithm, we can accomplish greatest organize throughput because of which information can transmit unreservedly immediately.
Heterosis and recombination effects in Hampshire and Landrace swine: II. Performance and carcass traits.

Twelve different mating types among the Hampshire and Landrace breeds were used to determine direct, maternal, heterosis, and recombination effects for performance and carcass traits. Mating types used were two purebred, two F1, two F2, two F3, and four backcross. Carcass data were collected on 238 barrows and 262 gilts over four replications. Traits measured were length (LENG), 10th rib off midline backfat (BF10), longissimus muscle area (LMA), and dressing percentage (DRS%). Average backfat (AVBF) was calculated as the mean of three midline fat depths measured opposite the first rib, last rib, and last lumbar vertebra. The model used to evaluate the carcass traits included main effects of mating type, farrowing season, and sex and included slaughter weight as a covariate. The performance traits of ADG, feed efficiency (FE), daily feed consumption (DFC), lean gain per day (LNGN), and lean efficiency (LNEF) were measured on a pen basis. Comparisons of reciprocal F1 crosses showed that carcasses from pigs sired by Hampshire boars were leaner and had more LMA than those sired by Landrace boars. Heterosis percentages were significant for AVBF (7.2%; P less than .01), BF10 (8.8%; P less than .01), DRS% (1.5%; P less than .01), ADG (11.5%; P less than .01), DFC (10.2%; P less than .01), LNGN (10.6%; P less than .01), and LNEF (6.0%; P less than .05). Epistatic recombination losses in the offspring were significant for LENG (3.6 cm; P less than .05) and approached significance for BF10 (6.1 mm; P less than .10).
Neuromuscular Specializations within Human Pharyngeal Constrictor Muscles

Objectives: At present it is believed that the pharyngeal constrictor (PC) muscles are innervated by the vagus (X) nerve and are homogeneous in muscle fiber content. This study tested the hypothesis that adult human PCs are divided into 2 distinct and specialized layers: A slow inner layer (SIL), innervated by the glossopharyngeal (IX) nerve, and a fast outer layer (FOL), innervated by nerve X. Methods: Eight normal adult human pharynges (16 sides) obtained from autopsies were studied to determine 1) their gross motor innervation by use of Sihler's stain; 2) their terminal axonal branching by use of acetylcholinesterase (AChE) and silver stain; and 3) their myosin heavy chain (MHC) expression in PC muscle fibers by use of immunocytochemical and immunoblotting techniques. In addition, the specialized nature of the 2 PC layers was also studied in developmental (newborn, neonate, and senescent humans), pathological (adult humans with idiopathic Parkinson's disease [IPD]), and comparative (nonhuman primate [adult macaque monkey]) specimens. Results: When nerves IX and X were traced from their cranial roots to their intramuscular termination in Sihler's-stained specimens, it was seen that nerve IX supplied the SIL, whereas branches of nerve X innervated the FOL in the adult human PCs. Use of AChE and silver stain confirmed that nerve IX branches supplying the SIL contained motor axons and innervated motor end plates. In addition to distinct motor innervation, the SIL contained muscle fibers expressing slow-tonic and α-cardiac MHC isoforms, whereas the FOL contained muscle fibers expressing developmental MHC isoforms. In contrast, the FOL became obscured in the elderly and in the adult humans with IPD because of an increased proportion of slow muscle fibers. Notably, distinct muscle fiber layers were not found in the human newborn and nonhuman primate (monkey), but were identified in the 2-year-old human. Conclusions: Human PCs appear to be organized into functional fiber layers, as indicated by distinct motor innervation and specialized muscle fibers. The SIL appears to be a specialized layer unique to normal humans. The presence of the highly specialized slow-tonic and α-cardiac MHC isoforms, together with their absence in human newborns and nonhuman primates, suggests that the specialization of the SIL may be related to speech and respiration. This specialization may reflect the sustained contraction needed in humans to maintain stiffness of the pharyngeal walls during respiration and to shape the walls for speech articulation. In contrast, the FOL is adapted for rapid movement as seen during swallowing. Senescent humans and patients with IPD are known to be susceptible to dysphagia, and this susceptibility may be related to the observed shift in muscle fiber content.
Adenocarcinoma in situ of the uterine cervix: an experience with 100 cases.

OBJECTIVE The aim of this study was to ascertain whether cold knife conization alone for cervical adenocarcinoma in situ is safe. METHODS One hundred consecutive patients with a histologically proven adenocarcinoma in situ (AIS) of the cervix were studied from 1970 to 1992. RESULTS Ninety-two women presented with abnormal smears, and of these 56% contained abnormal glandular cells. Sixty-seven (74%) of 90 women who underwent colposcopy had an abnormal examination, but a glandular abnormality was suspected in only 19 (28%). In all, 80 cold knife conizations were performed. In 7, no abnormality was found following punch biopsy. The margins were free of disease in 55 (75%). The most commonly involved margin in the remainder was the apical. Conization was followed by hysterectomy in 20 women: in 8 of these the cone margins were free and residual disease was found in 2 of the extirpated uteri: as these were extramural cases, inadequate sampling could not be excluded. Of the 12 women where hysterectomy followed conization with diseased margins, 9 had residual disease in the hysterectomy specimen. The definitive therapy was cold knife conization in 56 patients, hysterectomy in 38, and electrocoagulation diathermy in 6. Follow-up of the 53 patients treated by conization alone ranging from 1 to 16 years, with a mean of 8 years (3 have been lost to follow-up) revealed no recurrence of AIS or adenocarcinoma to date. CONCLUSION It is concluded that cold knife conization is a safe therapeutic modality, provided that the cone biopsy has been adequately sampled and the margins are free.