Miracle-dz/UniFAR-FTUs huggingface dataset:
Exploring Sphingolipid Implications in Neurodegeneration

Over the past decade, it was found that relatively simple sphingolipids, such as ceramide, sphingosine, sphingosine-1-phosphate, and glucosylceramide play important roles in neuronal functions by regulating rates of neuronal growth and differentiation. Homeostasis of membrane sphingolipids in neurons and myelin is essential to prevent the loss of synaptic plasticity, cell death and neurodegeneration. In our review we summarize data about significant brain cell alterations of sphingolipids in different neurodegenerative diseases such as Alzheimer's disease, Parkinson disease, Amyotrophic Lateral Sclerosis, Gaucher's, Farber's diseases, etc. We reported results obtained in brain tissue from both animals in which diseases were induced and humans in autopsy samples. Moreover, attention was paid on sphingolipids in biofluids, liquor and blood, from patients. In Alzheimer's disease sphingolipids are involved in the processing and aggregation of β-amyloid and in the transmission of the cytotoxic signal β-amyloid and TNFα-induced. Recently, the gangliosides metabolism in transgenic animals and the relationship between blood sphingolipids changes and cognitive impairment in Alzheimer's disease patients have been intensively studied. Numerous experiments have highlighted the involvement of ceramide and monohexosylceramide metabolism in the pathophysiology of the sporadic forms of Parkinson's disease. Moreover, gene mutations of the glucocerebrosidase enzyme were considered as responsible for Parkinson's disease via transition of the monomeric form of α-synuclein to an oligomeric, aggregated toxic form. Disturbances in the metabolism of ceramides were also associated with the appearance of Lewy's bodies. Changes in sphingolipid metabolism were found as a manifestation of Amyotrophic Lateral Sclerosis, both sporadic and family forms, and affected the rate of disease development. Currently, fingolimod (FTY720), a sphingosine-1-phosphate receptor modulator, is the only drug undergoing clinical trials of phase II safety for the treatment of Amyotrophic Lateral Sclerosis. The use of sphingolipids as new diagnostic markers and as targets for innovative therapeutic strategies in different neurodegenerative disorders has been included.
Cell Proliferation to Evaluate Preliminarily the Presence of Enduring Self-Regenerative Antioxidant Activity in Cerium Doped Bioactive Glasses

(1) Background: a cell evaluation focused to verify the self-regenerative antioxidant activity is performed on cerium doped bioactive glasses. (2) Methods: the glasses based on 45S5 Bioglass®, are doped with 1.2 mol%, 3.6 mol% and 5.3 mol% of CeO2 and possess a polyhedral shape (~500 µm2). Glasses with this composition inhibit oxidative stress by mimicking catalase enzyme (CAT) and superoxide dismutase (SOD) activities; moreover, our previous cytocompatibility tests (neutral red (NR), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Bromo-2-deoxyUridine (BrdU)) reveal that the presence of cerium promotes the absorption and vitality of the cells. The same cytocompatibility tests were performed and repeated, in two different periods (named first and second use), separated from each other by four months. (3) Results: in the first and second use, NR tests indicate that the presence of cerium promotes once again cell uptake and viability, especially after 72 h. A decrease in cell proliferation it is observed after MTT and BrdU tests only in the second use. These findings are supported by statistically significant results (4) Conclusions: these glasses show enhanced proliferation, both in the short and in the long term, and for the first time such large dimensions are studied for this kind of study. A future prospective is the implantation of these bioactive glasses as bone substitute in animal models.
Nanoparticles: An Experimental Study of Zinc Nanoparticles Toxicity on Marine Crustaceans. General Overview on the Health Implications in Humans

The presence of products containing nanoparticles or nanofibers is rapidly growing. Nanotechnology involves a wide spectrum of industrial fields. There is a lack of information regarding the toxicity of these nanoparticles in aqueous media. The potential acute toxicity of ZnO NPs using two marine crustacean species: the copepod Tigriopus fulvus and the amphypod Corophium insidiosum was evaluated. Acute tests were conducted on adults of T. Fulvus nauplii and C. insidiosum. Both test species were exposed for 96 h to 5 increasing concentrations of ZnO NPs and ZnSO4H2O, and the endpoint was mortality. Statistical analysis revealed that the mean LC50 values of both ZnO NPs and ZnSO4H2O (ZnO NPs: F = 59.42; P < 0.0015; ZnSO4H2O: F = 25.57; P < 0.0015) were significantly lower for Tigriopus fulvus than for Corophium insidiosum. This result confirms that the toxic effect could be mainly attributed to the Zn ions, confirming that the dissolution processes play a crucial role in the toxicity of the ZnO NPs.
Evolutionary life history theory as an organising framework for cohort studies: insights from the Cebu Longitudinal Health and Nutrition Survey

ABSTRACT By tracking a group of individuals through time, cohort studies provide fundamental insights into the developmental time course and causes of health and disease. Evolutionary life history theory seeks to explain patterns of growth, development, reproduction and senescence, and inspires a range of hypotheses that are testable using the longitudinal data from cohort studies. Here we review two decades of life history theory-motivated work conducted in collaboration with the Cebu Longitudinal Health and Nutrition Survey (CLHNS), a birth cohort study that enrolled more than 3000 pregnant women in the Philippines in 1983 and has since followed these women, their offspring and grandoffspring. This work has provided evidence that reproduction carries “costs” to cellular maintenance functions, potentially speeding senescence, and revealed an unusual form of genetic plasticity in which the length of telomeres inherited across generations is influenced by reproductive timing in paternal ancestors. Men in Cebu experience hormonal and behavioural changes in conjunction with changes in relationship and fatherhood status that are consistent with predictions based upon other species that practice bi-parental care. The theoretical expectation that early life cues of mortality or environmental unpredictability will motivate a “fast” life history strategy are confirmed for behavioural components of reproductive decision making, but not for maturational tempo, while our work points to a broader capacity for early life developmental calibration of systems like immunity, reproductive biology and metabolism. Our CLHNS findings illustrate the power of life history theory as an integrative, lifecourse framework to guide longitudinal studies of human populations.
Progress towards development of biologically-based strategies for the management of apple replant disease.

Effective non-fumigant biologically-based methods for control of apple replant disease have been lacking. Studies were conducted to examine the utility of approaches which modulate resident microbial communities as a potential means to provide disease suppression. Cover cropping sequences and use of brassicaceous soil amendments were employed to elicit desired microbial transformations, as well as to provide direct disease suppression. Wheat cropping provided disease control and enhanced fruit yield, but Brassica napus seed meal (RSM) amendment in concert with a post-plant mefenoxam soil drench was the sole treatment to provide a growth and yield response equivalent to soil fumigation. RSM-induced control of Rhizoctonia solani resulted from the enhanced activity of resident soil microorganisms, specifically Streptomyces spp. , rather than biofumigation, the mechanism commonly attributed to brassicaceous residue-induced soilborne disease control.
Variation in Phenology, Growth, and Wood Anatomy of Toona sinensis and Toona ciliata in Relation to Different Environmental Conditions

Tree‐ring proxy data from subtropical to tropical Australasia are valuable though rare sources for climate reconstructions. Toona sinensis (A. Juss.) M. Roem. and Toona ciliata M. Roem. occurring naturally in this region are among the most promising tree species for future tree‐ring research. However, little is known about their phenological behaviors and the influence of environmental conditions on their intraseasonal growth and wood anatomical properties. Growth experiments were conducted on young trees of both species to investigate their responses to different treatments. The results show that phenology and growth were adjusted according to the severity of the treatments. Restricted growth conditions often caused longer leafless periods, shorter flushes of leaves, and decreased height and diameter growth increments, and they resulted in more but smaller vessels. Under optimum conditions, T. ciliata did not become leafless, had multiple leaf flushes, sustained growth throughout the experiment, and did not form a tree‐ring boundary. All other specimens of both species entering leafless or at least semileafless periods formed one tree‐ring boundary during the experiment. The growth reaction was more distinct in the latewood than in the earlywood, in extreme cases suppressing the latewood totally. Although the experiment was conducted on young trees and hence should not simply be generalized and applied to adult specimens, the results indicate that both species are well suited for reliable future dendroclimatological investigations. Such studies need to pay attention to possible missing rings in the young parts of tree‐ring series.
Biosignatures Associated with Freshwater Microbialites

Freshwater microbialites (i.e., lithifying microbial mats) are quite rare in northern latitudes of the North American continent, with two lakes (Pavilion and Kelly Lakes) of southeastern BC containing a morphological variety of such structures. We investigated Kelly Lake microbialites using carbon isotope systematics, phospholipid fatty acids (PLFAs) and quantitative PCR to obtain biosignatures associated with microbial metabolism. δ13CDIC values (mean δ13CDIC −4.9 ± 1.1‰, n = 8) were not in isotopic equilibrium with the atmosphere; however, they do indicate 13C-depleted inorganic carbon into Kelly Lake. The values of carbonates on microbialite surfaces (δ13C) fell within the range predicted for equilibrium precipitation from ambient lake water δ13CDIC (−2.2 to −5.3‰). Deep microbialites (26 m) had an enriched δ13Ccarb value of −0.3 ± 0.5‰, which is a signature of photoautotrophy. The deeper microbialites (>20 m) had higher biomass estimates (via PLFAs), and a greater relative abundance of cyanobacteria (measured by 16S copies via qPCR). The majority of PLFAs constituted monounsaturated and saturated PLFAs, which is consistent with gram-negative bacteria, including cyanobacteria. The central PLFA δ13C values were highly depleted (−9.3 to −15.7‰) relative to δ13C values of bulk organic matter, suggesting a predominance of photoautotrophy. A heterotrophic signature was also detected via the depleted iso- and anteiso-15:0 lipids (−3.2 to −5.2‰). Based on our carbonate isotopic biosignatures, PLFA, and qPCR measurements, photoautotrophy is enriched in the microbialites of Kelly Lake. This photoautotrophy enrichment is consistent with the microbialites of neighboring Pavilion Lake. This indication of photoautotrophy within Kelly Lake at its deepest depths raises new insights into the limits of measurable carbonate isotopic biosignatures under light and nutrient limitations.
How CEO Ethical Leadership Influences Top Management Team Creativity: Evidence From China

The creative thinking and ability of top management team (TMT) members is important in coping with rapid changes in the external environment and improving the competitive advantage of an organization. This research focuses on the CEO–TMT interface to explain how CEOs influence TMT characteristics, which in turn affects TMT outcomes. Based on social learning theory, this study examines the associations among CEO ethical leadership, TMT cohesion, and TMT creativity in a Chinese context using a total of 91 TMTs. To verify the reliability and validity of the constructs, a series of confirmatory factor analyses (CFAs) were run. The results showed that the hypothetical model captured distinct constructs and fits the data well. A multistep regression method was used to test hypotheses. The results indicated that: (a) CEO ethical leadership has a positive effect on TMT creativity; (b) TMT cohesion plays a mediating role in the relationship between CEO ethical leadership and TMT creativity; and (c) power distance plays a moderating role in the relationship between CEO ethical leadership and TMT creativity. The greater the power distance, the weaker the positive relationship between CEO ethical leadership and TMT creativity. This study demonstrates the value of CEO ethical leadership and advocates the importance of establishing team cohesion and building a psychologically safe environment to motivate top managers within an organization to share information and knowledge to improve creativity.
Airway Remodeling in Asthma

Asthma is an inflammatory disease of the airways that may result from exposure to allergens or other environmental irritants, resulting in bronchoconstriction, wheezing, and shortness of breath. The structural changes of the airways associated with asthma, broadly referred to as airway remodeling, is a pathological feature of chronic asthma that contributes to the clinical manifestations of the disease. Airway remodeling in asthma constitutes cellular and extracellular matrix changes in the large and small airways, epithelial cell apoptosis, airway smooth muscle cell proliferation, and fibroblast activation. These pathological changes in the airway are orchestrated by crosstalk of different cell types within the airway wall and submucosa. Environmental exposures to dust, chemicals, and cigarette smoke can initiate the cascade of pro-inflammatory responses that trigger airway remodeling through paracrine signaling and mechanostimulatory cues that drive airway remodeling. In this review, we explore three integrated and dynamic processes in airway remodeling: (1) initiation by epithelial cells; (2) amplification by immune cells; and (3) mesenchymal effector functions. Furthermore, we explore the role of inflammaging in the dysregulated and persistent inflammatory response that perpetuates airway remodeling in elderly asthmatics.
Prevalence of facemask use among general public when visiting wet market during Covid-19 pandemic: An observational study.

Background In late December 2019, an outbreak of a novel coronavirus disease (COVID-19; previously known as 2019-nCoV) was epidemiologically linked to seafood and wet animal wholesale market in Wuhan, Hubei, China. This has instigated stigma among the general population as the wet market is viewed as a high-risk location for getting infected with coronavirus. Objective This study investigated the prevalence of facemask use among general population visiting the wet market. This study also investigated the demographic factors contributing to unacceptable facemask practice. Setting This prospective observational study was done among visitor to a district wet market selling range of live or freshly slaughtered animals during COVID-19 pandemic outbreak. Methods Individuals entering through dedicated entry point were observed for the type, category and practice of wearing personal protective equipment. Inclusion criteria for this study were any individuals entering the wet market. Subjects were categorized into two groups of acceptable and unacceptable facemask practice. The Pearson chi-square was used to test for differences in investigated variables in the univariate setting and Binary Logistic regression model was used in the multivariate setting. Main outcome measure Prevalence, acceptance practice and odds ratio of unacceptance of facemask use. Results Among 1697 individuals included in the final analysis, 1687 (99.7%) was observed wearing facemask with 1338 (78.8%) using medical-grade facemask. Among them, 1615 (95.7%) individuals facemask practice was acceptable while the reaming 72 (4.3%) individuals were observed with unacceptable facemask practice. Individuals using medical-grade facemask and high-risk age group are 6.4 times (OR=6.40; 95% CI, 2.00-20.43; p=.002) and 2.06 times practice (OR=2.06; 95% CI, 1.08-3.94; p=.028). More likely to practice unacceptable facemask use respectively. Conclusion High saturation of facemask among the general population is an adequate indicator of public hygiene measures strategy which can help to mitigate the COVID-19 epidemic impact. Alarmingly, the unacceptable facemask practice among high-risk population raises the need for a targeted approach by healthcare authorities to ensure satisfactory facemask use.
The Influence of Small Intestinal Bacterial Overgrowth in Digestive and Extra-Intestinal Disorders

Small intestinal bacterial overgrowth (SIBO) is a condition hallmarked by an increase in the concentration of colonic-type bacteria in the small bowel. Watery diarrhea, bloating, abdominal pain and distension are the most common clinical manifestations. Additionally, malnutrition and vitamin (B12, D, A, and E) as well as minerals (iron and calcium) deficiency may be present. SIBO may mask or worsen the history of some diseases (celiac disease, irritable bowel disease), may be more common in some extra-intestinal disorders (scleroderma, obesity), or could even represent a pathogenetic link with some diseases, in which a perturbation of intestinal microbiota may be involved. On these bases, we performed a review to explore the multiple links between SIBO and digestive and extra-intestinal diseases.
Studies on the life history of some species of Protocalliphora (Diptera: Calliphoridae)

The rate of development of the larvae, postfeeding larvae, puparia, and adults of 10 species of Protocalliphora were studied and differences noted in the rates of development and (or) survival. Larvae of Protocalliphora were photonegative and strongly influenced by thigmotactic stimuli. The method of feeding of the larva is described. Metamorphosis was completed more quickly at higher temperatures than at lower temperatures. Survival of purparia at temperatures of 7 °C or lower was poor and indicated that this stage was not adapted to withstand low temperatures and probably did not survive the winter. Adult Protocalliphora females were longer lived than males, surviving for an average of 70–100 days, with some individuals surviving for over 250 days under laboratory conditions. Adults fed readily on a sugar–protein diet, crushed berries, and certain species of flowers, but most species did not feed on any form of carrion. Adults mated readily in captivity, but none of the seven species tested crossbred. S.
Guidelines for the Use of Antiretroviral Agents in Pediatric HIV Infection, January 7, 2000

Abstract These guidelines were developed by the Working Group on Antiretroviral Therapy and Medical Management of HIV Infected Children convened by the National Pediatric and Family HIV Resource Center (NPHRC), the Health Resources and Services Administration (HRSA), and the National Institutes of Health (NIH). The Co-Chairs of the Working Group were James Oleske, MD, MPH, University of Medicine and Dentistry of New Jersey (UMDNJ)-New Jersey Medical School, Newark, New Jersey, and Gwendolyn B. Scott, MD, University of Miami School of Medicine, Miami, Florida.
The expression of opsins in the human skin and its implications for photobiomodulation: A Systematic Review

Skin is the organ most extensively exposed to light of a broad range of wavelengths. Several studies have reported that skin expresses photoreceptive molecules called opsins. However, the identity and functional role of opsins in the human skin remain elusive. We aim to summarize current scientific evidence on the types of opsins expressed in the skin and their biological functions.
Benchmarking Best NPD Practices—I

OVERVIEW: This first of three articles reports the results of the most recent American Productivity and Quality Center study on performance and best practices in new product development. Performance results achieved on a number of NPD metrics are reported first, followed by a set of Best Performing businesses, what these businesses have in common and—most important—what differentiates them from the rest. This first article focuses on the “people side” of NPD—on how project teams are organized and on other winning characteristics of teams. The climate and culture for innovation within the business proves to be one of the strongest drivers of NPD performance, and the specifics of what the Best Performers have done to achieve this positive climate are outlined. Finally the role of senior management in setting the stage for the business's NPD effort is examined.
A seasonal cycle in the carbon‐isotope composition of organic carbon in the Sanaga River, Cameroon

We report measurements of the carbon‐isotope composition (δ13C value) of particulate organic carbon (POC) in the suspended sediment load of the Sanaga and Mbam Rivers, Cameroon. The results demonstrate that there is a seasonal cycle in δ13C value of 3.3%0 (−26.6 to −23.3%0) in the Sanaga River and 5.9%0 (−27.1 to −21.2%0) in the Mbam River that is directly related to seasonal changes in the discharge of the rivers. During periods of high discharge, δ13C values of POC are high, reflecting an increase in the proportion of POC derived from C, plants, including C4‐derived carbon transported overland by wet season rains from savanna regions in the catchment hinterland. During periods of low discharge, the δ13C values of POC are low, reflecting derivation of the carbon primarily from C4 plants growing close to the riverbanks. Results suggest that the prime determinant of POC δ13C values in tropical rivers that carry a significant suspended sediment load is the δ13C value of the catchment vegetation.
Reduction of aflatoxin in fermented milks during production and storage

Aflatoxins are a group of secondary fungal metabolites produced by toxigenic strains of Aspergillus flavus, A. parasiticus and A. nomius. They are one of the most common food contamination problems. Aflatoxins M1 and M2 are the hydroxylated metabolites of aflatoxins B1 and B2 and emerge in raw milk when lactating animals ingest feed contaminated with these mycotoxins. They may contaminate other dairy products including fermented milks. Aflatoxins are fairly stable in many foods and are rather resistant to degradation. Its stability can be affected by different factors. This article mainly reviews the impact of process, formulation and storage factors on the amount of aflatoxins in fermented milks while consider related mechanisms.