Miracle-dz/UniFAR-FTUs huggingface dataset:
Seroprevalence against COVID-19 and follow-up of suspected cases in primary health care in Spain

Background During the coronavirus disease 2019 (COVID-19) pandemic little information has been available about patients with mild or moderate symptoms attended and followed in the primary care setting, most of whom had an unknown status for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Objectives We aim to measure the seroprevalence of antibodies against SARS-CoV-2 infection in a community sample of asymptomatic individuals and among symptomatic patients (without confirmed diagnosis) followed in a primary care setting. As a secondary objective, we estimated the proportions of symptomatic patients seeing at an emergency department (ED), hospitalized or dying, and identified the most important clinical symptoms associated with a positive infection. Methods From April 21 to April 24 2020, we selected a random sample of 600 individuals stratified by age groups, from a total population of 19,899 individuals from a community area in Barcelona (study population 1). From April 29 to May 5 2020, we also invited all the patients that had been followed by general practitioners (GPs) (study population 2). We used for both populations COVID-19 Rapid lateral flow immunoassay which qualitatively assesses the presence of patient-generated IgG and IgM in approximately 10-15 minutes. The prevalence (95% confidence intervals [CI]) of infection (past and current) was defined as the proportion of individuals with antibody seropositivity. Odds ratios (ORs) for a positive test result were estimated using logistic regression analysis. Results Three hundred and eleven asymptomatic individuals from the randomly selected sample accepted to participate in the study. The overall mean age was 43.7 years (SD 21.79, range 1-94) and 55% were women. Seventeen individuals were seropositive for IgM and/or IgG, resulting an overall prevalence of 5,47% (95% CI, 3.44-8.58). Six-hundred and thirty-four symptomatic patients were followed by GPs. The overall mean age was 46.97 years (SD 20.05, range 0-92) and 57.73% were women. Of these, 244 patients (38.49%) were seropositive for IgM and/or IgG. During the follow-up period, 27.13% of symptomatic patients attended the ED, 11.83% were hospitalized and about 2% died. Results of the multivariate logistic regression analysis showed that the OR for a positive test was significantly increased in patients who had fever (>38{degrees}C), ageusia and contact with a patient diagnosed with COVID-19. Conclusions The seroprevalence of antibodies against SARS-CoV-2 among asymptomatic individuals in the general population was lower than expected. Approximately 40% of the symptomatic patients followed by GPs during the peak months of the pandemic in Barcelona, were positive. Fever (>38{degrees}C), anosmia, ageusia and contact with a patient diagnosed with COVID-19 were associated with a positive test result.
Losing Ground

Throughout much of the twentieth century, Harlem was unquestionably the symbolic capital of Black America. Though it has been half-a-century since the community lost its designation as the most populous Black urban center, Harlem remains the quintessential expression of the African American cultural experience for much of the world. The community’s significance, however, goes beyond the musicians, poets, and artists of the Harlem Renaissance. Harlem also anchored a unique political history. It was not only a place to which people of African descent from all over the world were drawn, but it was also a space where people made history through political struggle. This article will explore some of the processes that constrained Harlem’s historymaking capacity in the 1980s and 1990s. We will discuss a community whipsawed by the drug epidemic and the so-called war on drugs, and the impacts of these events on sustained political movement. We conclude with a discussion of Harlem’s potential to make history anew, whether its legacy of activism and community protest can be reinvigorated to once again push society at large toward more equitable structures.
Pre and Post-Operative Computer Tomography Findings of a patient with a Type A Aortic Dissection Extending from the Aortic Valve to Iliac Vessels

Aortic dissection is a high morbidity, high mortality, medical catastrophe. It is twice as common as ruptured abdominal aortic aneurysm and the mortality following acute aortic dissection is often quoted in hours. The presentation of Aortic Dissection is variable and can be diagnostically challenging, which can have a direct effect on mortality in often young and relatively healthy patients. We present the case of a young man who presented to the emergency departments with central chest pain and ST depression laterally on his ECG. A computer tomography (CT) scan revealed The CT scan showed a proximal Type A dissection involving the aortic valve and the coronary arteries, extending down to the iliac vessels. The patient was transferred to a cardiothoracic centre to undergo emergency surgery and had repair of the aortic dissection, he made a good post-operative recovery and has no residual neurological deficit. A further computer tomography scan was performed 8 months later which showed no change in the patient vascular anatomy.
Computational Modeling of Inhibitory Transsynaptic Signaling in Hippocampal and Cortical Neurons Expressing Intrabodies Against Gephyrin

GABAergic transmission regulates neuronal excitability, dendritic integration of synaptic signals and oscillatory activity, thought to be involved in high cognitive functions. By anchoring synaptic receptors just opposite to release sites, the scaffold protein gephyrin plays a key role in these tasks. In addition, by regulating GABAA receptor trafficking, gephyrin contributes to maintain, at the network level, an appropriate balance between Excitation (E) and Inhibition (I), crucial for information processing. An E/I imbalance leads to neuropsychiatric disorders such as epilepsy, schizophrenia and autism. In this article, we exploit a previously published computational method to fit spontaneous synaptic events, using a simplified model of the subcellular pathways involving gephyrin at inhibitory synapses. The model was used to analyze experimental data recorded under different conditions, with the main goal to gain insights on the possible consequences of gephyrin block on IPSCs. The same approach can be useful, in general, to analyze experiments designed to block a single protein. The results suggested possible ways to correlate the changes observed in the amplitude and time course of individual events recorded after different experimental protocols with the changes that may occur in the main subcellular pathways involved in gephyrin-dependent transsynaptic signaling.
How Are Mechanical, Physiological, and Perceptual Variables Affected by the Rest Interval Between Sets During a Flywheel Resistance Session?

The use of flywheel devices has increased in popularity during the last few years. Flywheel training is an attractive alternative to traditional resistance exercise because it allows for the loading stimulus to be manipulated. Some of the benefits associated with flywheel training include increases in muscle hypertrophy, muscular strength and reductions in injury risk. Nevertheless, there is a lack of research about how basic training variables [i.e., rest intervals (RI) between sets], or variables associated with manipulation of the loading stimulus (i.e., different inertial loads), influence the acute responses during a flywheel session. Thus, the aim of this study was to assess the influence of three different RI between sets (1, 2, or 3 min), during a flywheel squat session with two different inertial loads: light (0.025 kg⋅m2) and high (0.075 kg⋅m2). Twenty-three participants performed six exercise sessions (two inertial loads × three RI) consisting of four sets of 11 repetitions. Concentric and eccentric power, lactate concentration ([La]) and ratings of perceived exertion (RPE) were measured during the exercise session, and delayed onset muscular soreness (DOMS) were recorded 24 h post-exercise. Both concentric (9.1 and 22.1% at light and high load respectively; p = 0.022 and 0.005) and eccentric peak power (17.5% at high load; p = 0.02) decreased across sets when the 1 min RI was used. Concentric peak power was decreased (11.1%, p = 0.041) from the 2 min RI only with the high inertial load. RPE was higher during the 1 min compared with the 3 min RI protocol when using the high inertial load (p = 0.028). [La] was higher when using the 2 min RI compared with the 3 min RI at light load (p = 0.03). In conclusion, during flywheel training sessions, a short RI (1 min) was insufficient to maintain power output across all four sets and was linked to greater perceptual variables. A 2 min RI allowed for power to be maintained, but only when training with light inertial loads. Based on these results, coaches should consider prescribing 3 min RI’s when performing flywheel squat exercises regardless the inertial load. In contrast, when using 2 min RI’s the inertial load should be light.
Feasibility, Acceptability, and Clinical Implementation of an Immersive Virtual Reality Intervention to Address Psychological Well-Being in Children and Adolescents With Cancer

Objective: Virtual reality (VR), a novel and highly immersive technology, offers promise in addressing potential psychological impacts of cancer treatments and hospitalization. The primary aim of this study was to examine multiple key user perspectives on the acceptability and feasibility of an Immersive VR therapeutic intervention for use with hospitalized patients with cancer. Secondary aims were to identify issues and opportunities related to the adoption and clinical implementation of VR in pediatric oncology settings. Method: The study was conducted at The Royal Children’s Hospital (RCH), Melbourne, Australia. Thirty multidisciplinary oncology health care professionals participated in an initial test of VR intervention usability (Stage 1). Ninety oncology inpatients (7-19 years) and their parent caregivers participated in a pilot randomized controlled study to examine the effectiveness of an Immersive VR therapeutic intervention (Stage 2). This mixed methods study reports Stages 1 and 2 quantitative and qualitative data related to VR feasibility and acceptability. Results: Results indicate favorable perceptions from health care professionals with respect to ease of use and usefulness of VR, and had positive intentions to use it in the future. Parent caregivers reported high acceptability of VR for their hospitalized child. Patients reported high satisfaction of the VR intervention within minimal adverse effects. Barriers and facilitators to VR use with seriously ill children and specific recommendations for content development were elicited. Conclusion: This study shows that there are several potential clinical uses for Immersive VR intervention, beyond medical procedural distraction, to support psychological adjustment to hospitalization and patient quality of life.
Tomato Diseases and Pests Detection Based on Improved Yolo V3 Convolutional Neural Network

Tomato is affected by various diseases and pests during its growth process. If the control is not timely, it will lead to yield reduction or even crop failure. How to control the diseases and pests effectively and help the vegetable farmers to improve the yield of tomato is very important, and the most important thing is to accurately identify the diseases and insect pests. Compared with the traditional pattern recognition method, the diseases and pests recognition method based on deep learning can directly input the original image. Instead of the tedious steps such as image preprocessing, feature extraction and feature classification in the traditional method, the end-to-end structure is adopted to simplify the recognition process and solve the problem that the feature extractor designed manually is difficult to obtain the feature expression closest to the natural attribute of the object. Based on the application of deep learning object detection, not only can save time and effort, but also can achieve real-time judgment, greatly reduce the huge loss caused by diseases and pests, which has important research value and significance. Based on the latest research results of detection theory based on deep learning object detection and the characteristics of tomato diseases and pests images, this study will build the dataset of tomato diseases and pests under the real natural environment, optimize the feature layer of Yolo V3 model by using image pyramid to achieve multi-scale feature detection, improve the detection accuracy and speed of Yolo V3 model, and detect the location and category of diseases and pests of tomato accurately and quickly. Through the above research, the key technology of tomato pest image recognition in natural environment is broken through, which provides reference for intelligent recognition and engineering application of plant diseases and pests detection.
Long Non-coding RNA and mRNA Profile of Liver Tissue During Four Developmental Stages in the Chicken

The liver is the major organ of lipid biosynthesis in the chicken. In laying hens, the liver synthesizes most of the yolk precursors and transports them to developing follicles to produce eggs. However, a systematic investigation of the long non-coding RNA (lncRNA) and mRNA transcriptome in liver across developmental stages is needed. Here, we constructed 12 RNA libraries from liver tissue during four developmental stages: juvenile (day 60), sexual maturity (day 133), peak laying (day 220), and broodiness (day 400). A total of 16,930 putative lncRNAs and 18,260 mRNAs were identified. More than half (53.70%) of the lncRNAs were intergenic lncRNAs. The temporal expression pattern showed that lncRNAs were more restricted than mRNAs. We identified numerous differentially expressed lncRNAs and mRNAs by pairwise comparison between the four developmental stages and found that VTG2, RBP, and a novel protein-coding gene were differentially expressed in all stages. Time-series analysis showed that the modules with upregulated genes were involved in lipid metabolism processes. Co-expression networks suggested functional relatedness between mRNAs and lncRNAs; the DE-lncRNAs were mainly involved in lipid biosynthesis and metabolism processes. We showed that the liver transcriptome varies across different developmental stages. Our results improve our understanding of the molecular mechanisms underlying liver development in chickens.
“Anti-microbial activity of roots of Rauwolfia serpentina (Apocynaceae) collected from different geographical regions of India”

Antimicrobials are the natural killers of micro-organisms that invade them. The main benefits of these agents are their ability to cause selective toxicity on the target without hampering the natural equilibrium. Rauwolfia serpentina (Sarpagandha) is a glabrous perennial undershrub. It is given in many disorders like worm infestation, insomnia, epilepsy, fever, obesity, mental ailments, influence of evil spirit etc. It is reported that the plant also has antimicrobial activity. This article deals with the effect of geographical variation on the anti-microbial property of plant. The roots are collected from different zones of India. The water as well as ethanol extracts were studied against the pathogen Escherichia coli in Mueller-Hinton agar culture media. The results calculated from the inhibition zone revealed that the aqueous extract of five samples of roots of Rauwolfia serpentina shows the highest zone inhibition against the Escherichia coli. The highest zone inhibition is in the sample collected from North region in both ethanolic and aqueous extracts.
LAPPEENRANNAN-LAHDEN TEKNILLINEN YLIOPISTO LUT School of Business and Management Kauppatiede

Author: Matti Oskari Koivula Title: Entry mode decisions and changes of Finnish food cooperatives: Real options reasoning-based view Faculty: School of Business Major: International marketing management Year: 2019 Master’s thesis: Lappeenranta University of Technology Examiners: Sanna-Katriina Asikainen
Position of macula lutea and presence of proliferative vitreoretinopathy affect vitreous cytokine expression in rhegmatogenous retinal detachment

Our purpose was to evaluate the concentrations of vitreous cytokines in patients with rhegmatogenous retinal detachment (RRD). We hypothesized that patients with macula on RRD have lower levels of cytokines compared to patients with macula off RRD and proliferative vitreoretinopathy (PVR). Vitreous fluids were collected during 23G pars plana vitrectomy from 58 eyes of 58 patients. Indication for vitrectomy included macula off and macula on RRD, PVR, and idiopathic epiretinal membrane (ERM). A multiplex chemiluminescent immunoassay was performed to measure the concentrations of 48 cytokines, chemokines, and growth factors. Levels of HGF, IL-6, IL-8, IL-16, IFN-gamma, MCP-1, and MIF were significantly higher in all groups of retinal detachment compared to ERM. Levels of CTACK, eotaxin, G-CSF, IP-10, MIG, SCF, SCGF-beta, SDF-1alpha were significantly higher in PVR compared to macula on RRD and ERM. Levels of IL-1ra, IL-5, IL-9, M-CSF, MIP-1alpha, and TRIAL were significantly higher in PVR compared to macula on RRD. Our results indicate that the position of macula lutea and the presence of PVR significantly influence vitreous cytokine expression. The detected proteins may serve as biomarkers to estimate the possibility of PVR formation and may help to invent personalized therapeutic strategies to slow down or prevent PVR.
伴环状铁粒幼红细胞增多骨髓增生异常综合征基因突变特征及临床意义

目的 研究伴环状铁粒幼红细胞增多（RS）的骨髓增生异常综合征（MDS-RS）患者的基因突变特征及其临床意义。 方法 收集2001年1月至2019年6月于中国医学科学院血液病医院新诊断的255例原发性MDS-RS患者资料。 129例采用一代测序、126例采用包含112个血液肿瘤相关基因的二代测序（NGS）进行基因突变分析。 结果 共193例（75.7%）检出SF3B1突变，其中SF3B1 K700E突变147例（76.2%）。 非SF3B1基因突变较常见的有TET2（16.7%）、ASXL1（14.3%）、U2AF1（11.1%）、TP53（7.9%）、SETBP1（6.3%）和RUNX1（6.3%）。 RS 5%～<15%患者SETBP1突变率显著高于RS≥15%患者（21.4%对4. 5%，P＝0.044），其余基因突变率差异无统计学意义（P值均>0.05）。 在114例NGS检出SF3B1突变的患者中，RS 5%～<15%患者SF3B1等位基因突变频率（VAF）与骨髓RS比例呈正相关（r＝0.486，P＝0.078）；RS≥15%组SF3B1突变型患者骨髓RS比例显著高于野生型患者［40.0%（15.0%～80.0%）对25. 5%（15.0%～82.0%），P<0.001］，且SF3B1 VAF与骨髓RS比例呈正相关（P＝0.009，rs＝0.261）。 全部患者SF3B1突变型与野生型年龄、ANC、PLT、平均红细胞体积、RS比例、IPSS-R染色体核型及IPSS-R预后分组等方面差异有统计学意义（P值均<0.05）。 多因素分析显示，SF3B1突变是影响总生存（OS）时间的独立良好预后因素（HR＝0.265，95% CI 0.077～0.917，P＝0.036），TP53突变是独立不良预后因素（HR＝6.272，95% CI 1.725～22.809，P＝0.005）。 根据SF3B1和TP53突变状态将MDS-RS患者分为四组：SF3B1和TP53均突变组、SF3B1和TP53均野生组、SF3B1野生伴TP53突变组及SF3B1突变伴TP53野生组，四组患者OS时间差异显著（P<0.001）。 组间两两比较显示SF3B1突变伴TP53野生组OS时间显著长于SF3B1野生伴TP53突变组、SF3B1和TP53野生组，而与SF3B1和TP53均突变组比较差异无统计学意义。 结论 SF3B1突变在MDS-RS中的发生率较高，几乎均是错义突变，以K700E突变最为常见。 SF3B1突变是MDS-RS患者生存的独立良好预后因素，TP53突变是独立不良预后因素，二者联合可更精细地指导MDS-RS患者预后分层。
Structure of the RECK CC domain, an evolutionary anomaly

Significance Five small and homologous domains—referred to as CC domains—in the cell surface protein RECK play essential roles in signaling via the WNT7A and Wnt7B ligands in the context of central nervous system vascular development. We have determined the three-dimensional structure of one of these domains and find that it consists of four short α-helices stabilized by three disulfide bonds, a structure that has not been seen previously in the extracellular domain of any vertebrate cell surface or secreted protein. Homology searches of genome sequences show that the CC domain arose early in the evolution of multicellular animals, but, unlike many other small folded domains, it is only detected in a single gene (RECK). Five small protein domains, the CC-domains, at the N terminus of the RECK protein, play essential roles in signaling by WNT7A and WNT7B in the context of central nervous system angiogenesis and blood–brain barrier formation and maintenance. We have determined the structure of CC domain 4 (CC4) at 1.65-Å resolution and find that it folds into a compact four-helix bundle with three disulfide bonds. The CC4 structure, together with homology modeling of CC1, reveals the surface locations of critical residues that were shown in previous mutagenesis studies to mediate GPR124 binding and WNT7A/WNT7B recognition and signaling. Surprisingly, sequence and structural homology searches reveal no other cell-surface or secreted domains in vertebrates that resemble the CC domain, a pattern that is in striking contrast to other ancient and similarly sized domains, such as Epidermal Growth Factor, Fibronectin Type 3, Immunoglobulin, and Thrombospondin type 1 domains, which are collectively present in hundreds of proteins.
The Investigation of Simultaneous EEG and Eye Tracking Characteristics During Fixation Task in Mild Alzheimer’s Disease

Alzheimer’s disease (AD) is a neurodegenerative disorder that occurs many years before the first clinical symptoms. Finding more exact, significant, and valuable criteria or indices for the diagnosis of the mild form of Alzheimer’s disease is very important for clinical and research purposes. Electroencephalography (EEG) and eye tracking biomarkers would provide noninvasive tools for the early detection of AD. Due to the advantages of EEG and eye tracking, in this study, we employed them simultaneously to conduct research on the mild AD. For this purpose, 19 patients with mild AD were compared with 19 gender- and age-matched normal subjects who did not have any history of cognitive or neurological disorders. EEG and eye-tracking data were concurrently collected in both groups in a fixation task. Our results revealed that the total fixation duration was significantly shorter for the AD patients, but their fixation frequency was more than that of the controls. In addition, increased theta power and decreased alpha power were observed in the AD group. Interestingly, there was a statistically significant correlation between fixation frequency and alpha power in the parietal area in the control group. However, this connection was not statistically significant in the AD group. The findings also indicated an elevated coherence in the AD patients in the parieto-occipital area. It is assumed that the AD patients might use the neural compensational processes for the fixation state. This study provides evidence for the simultaneously EEG and eye-tracking changes in the areas, which are involved in the control of the fixational eye movements.
Loss of association between HbA1c and vascular disease in older adults with type 1 diabetes

Aims Robust evidence supports intensive glucose control in those with recently diagnosed type 1 diabetes to reduce the risk of developing micro- and macrovascular complications. Data to support longitudinal glycaemic targets is lacking. We aimed to explore if longer duration of diabetes and greater age might reduce the impact of glycaemia on the risk of vascular complications. Research and design methods Data for adults age 20 years or more, was extracted from a clinical database of people with type 1 diabetes cared for at a London teaching hospital. The presence or absence of micro- and macro-vascular complications was recorded. Multivariable logistic regression analysis was performed using HbA1c as independent variable, diabetes duration and age as continuous variable and obesity, hypertension, hypercholesterolaemia, low HDL cholesterol and hypertriglyceridaemia as categorical variables. Results Data from 495 patients was used. HbA1c above 60 mmol/mol (7.6%) was associated with increased microvascular complications in patients aged 20–44 years, independent of age and duration of diabetes. In older people with T1DM duration of diabetes was the major risk factor. Conclusions Our study suggests that increased age and greater duration of diabetes reduce the impact of glycaemia on the risk of vascular complications. Intensive blood glucose management in patients aged ≥45 years may have limited benefits in terms of reducing the risk of complications although this does not dismiss the benefits of good glycaemic control in older people with T1DM.