Miracle-dz/UniFAR-FTUs huggingface dataset:
Immunohistochemical analysis of the S100A1, S100B, CD44 and Bcl-2 antigens and the rate of cell proliferation assessed by Ki-67 antibody in benign and malignant melanocytic tumours

Malignant melanomas usually have an unfavourable prognosis and poor response to chemotherapy. Deregulation of cell proliferation, programmed cell death and intercellular interactions are among several important mechanisms that might lead to malignant transformation of melanocytes and melanoma progression. The S100A1, S100B, Bcl-2 and CD44 antigens have all been described as being involved in different processes of melanoma progression. The expression of these antigens, as well as the rate of cell proliferation, was analyzed retrospectively in melanocytic tumours from 126 patients (32 males and 94 females, age ranging from 11 to 91 years). The series included benign (45 intradermal, 27 compound and eight displastic naevi) and malignant (39 primary and 14 metastatic) melanocytic tumours. The proliferating rate assessed by Ki-67 staining was lower in naevi than in melanomas, with a correlation coefficient of r = 0814. There was no overlap for rate of proliferation between benign and malignant tumours. The expression of S100A1 was low in benign melanocytic tumours and increased in malignant melanomas (r = 0.61). In contrast, a higher percentage of S100B antigen-positive cells were observed in benign melanocytic lesions than in melanomas (Pearson correlation coefficient, 0.627). In addition, positive immunostaining for S100B antigen in malignant melanomas corresponded with the areas with increased proliferating rate. The expression of Bcl-2 was lower in melanomas than in benign melanocytic tumours (r = −0.53). Bcl-2-negative areas within melanomas had an increased proliferating rate. The expression of CD44 showed a large variation both in benign and malignant melanocytic tumours. CD44 antigen expression was higher in melanomas with known metastases than in those without metastases, but this difference was not statistically significant.
Melanoma candidate genes CDKN2A/p16/INK4A, p14ARF, and CDK4 sequencing in patients with uveal melanoma with relative high-risk for hereditary cancer predisposition

The reported frequencies of germline mutations in the melanoma candidate genes are low in patients with uveal melanoma (UM). However, the number of families studied is limited and the majority of the published reports used low-sensitivity techniques for mutational screening. Identifying the frequency of alterations in any of the melanoma genes in patients with UM with increased hereditary cancer risk is important for proper counseling of these patients. We studied a total of 47 patients with UM including three with a family history of UM, 18 with a family and/or personal history of cutaneous melanoma (CM), three with early age at diagnosis (<30), 11 with increased risk for a known familial cancer syndrome, and 12 with a second primary tumor. Germline screening for mutations in CDKN2A, p14ARF, and exon 2 of CDK4 was carried out by direct sequencing. We identified a variant (IVS1-69 C>T) of uncertain significance in exon 1b of p14ARF in one of the patients with UM and his mother who also had UM. The variant was neither detected in any of the other patients with UM nor in 146 controls. We did not identify pathogenic mutations in CDKN2A nor exon 2 of CDK4 gene. Our study supports the low frequency of germline mutation of the CM candidate genes in patients with UM with family histories suggestive of a high risk for hereditary cancer. Germline testing for CDKN2A might be reserved for patients with UM with a family history of two or more CM.
Formation of RNA Phosphodiester Bond by Histidine‐Containing Dipeptides

A new scenario for prebiotic formation of nucleic acid oligomers is presented. Peptide catalysis is applied to achieve condensation of activated RNA monomers into short RNA chains. As catalysts, L‐dipeptides containing a histidine residue, primarily Ser‐His, were used. Reactions were carried out in selforganised environment, a water‐ice eutectic phase, with low concentrations of reactants. Incubation periods up to 30 days resulted in the formation of short oligomers of RNA. During the oligomerisation, an active intermediate (dipeptide–mononucleotide) is produced, which is the reactive species. Details of the mechanism and kinetics, which were elucidated with a set of control experiments, further establish that the imidazole side chain of a histidine at the carboxyl end of the dipeptide plays a crucial role in the catalysis. These results suggest that this oligomerisation catalysis occurs by a transamination mechanism. Because peptides are much more likely products of spontaneous condensation than nucleotide chains, their potential as catalysts for the formation of RNA is interesting from the origin‐of‐life perspective. Finally, the formation of the dipeptide–mononucleotide intermediate and its significance for catalysis might also be viewed as the tell‐tale signs of a new example of organocatalysis.
4‐Hydroxy‐3‐methyl‐6‐(1‐methyl‐2‐oxoalkyl)pyran‐2‐one Synthesis by a Type III Polyketide Synthase from Rhodospirillum centenum

The purple photosynthetic bacterium Rhodospirillum centenum has a putative type III polyketide synthase gene (rpsA). Although rpsA was known to be transcribed during the formation of dormant cells, the reaction catalyzed by RpsA was unknown. Thus we examined the RpsA reaction in vitro, using various fatty acyl‐CoAs with even numbers of carbons as starter substrates. RpsA produced tetraketide pyranones as major compounds from one C10–14 fatty acyl‐CoA unit, one malonyl‐CoA unit and two methylmalonyl‐CoA units. We identified these products as 4‐hydroxy‐3‐methyl‐6‐(1‐methyl‐2‐oxoalkyl)pyran‐2‐ones by NMR analysis. RpsA is the first bacterial type III PKS that prefers to incorporate two molecules of methylmalonyl‐CoA as the extender substrate. In addition, in vitro reactions with 13C‐labeled malonyl‐CoA revealed that RpsA produced tetraketide 6‐alkyl‐4‐hydroxy‐1,5‐dimethyl‐2‐oxocyclohexa‐3,5‐diene‐1‐carboxylic acids from C14–20 fatty acyl‐CoAs. This class of compounds is likely synthesized through aldol condensation induced by methine proton abstraction. No type III polyketide synthase that catalyzes this reaction has been reported so far. These two unusual features of RpsA extend the catalytic functions of the type III polyketide synthase family.
Variable Selection via Regression Trees in the Presence of Irrelevant Variables

Many tree algorithms have been developed for regression problems. Although they are regarded as good algorithms, most of them suffer from loss of prediction accuracy when there are many irrelevant variables and the number of predictors exceeds the number of observations. We propose the multistep regression tree with adaptive variable selection to handle this problem. The variable selection step and the fitting step comprise the multistep method. The multistep generalized unbiased interaction detection and estimation (GUIDE) with adaptive forward selection (fg) algorithm, as a variable selection tool, performs better than some of the well-known variable selection algorithms such as efficacy adaptive regression tube hunting (EARTH), FSR (false selection rate), LSCV (least squares cross-validation), and LASSO (least absolute shrinkage and selection operator) for the regression problem. The results based on simulation study show that fg outperforms other algorithms in terms of selection result and computation time. It generally selects the important variables correctly with relatively few irrelevant variables, which gives good prediction accuracy with less computation time.
Seizures and Epilepsy in the Elderly.

Seizures and Epilepsy in the Elderly. Edited bya james rowan and r eugene ramsey. (Pp 343). Published by Butterworth-Heinemann, Oxford, 1997. ISBN 0-7506-9622-2. The incidence of epilepsy rises sharply over the age of 60 and with an ever-increasing elderly population, it is becoming an area of increasing clinical and social importance. The limitations of current knowledge, especially in relation to treatment and directions for future clinical research, are considered in a valuable penultimate chapter of this book, that would have been well placed at the beginning. The book …
Estimation Procedure for a Multiple Time Series Model

Given a multiple time series sharing common autoregressive patterns, we estimate an additive model. The autoregressive component and the individual random effects are estimated by integrating maximum likelihood estimation and best linear unbiased predictions in a backfitting algorithm. The simulation study illustrated that the estimation procedure provides an alternative to the Arellano–Bond generalized method of moments (GMM) estimator of the panel model when T > N and the Arellano–Bond generally diverges. The estimator has high predictive ability. In cases where T ≤ N, the backfitting estimator is at least comparable to Arellano–Bond estimator.
Hereditary Spastic Paraplegia Associated With Axonal Neuropathy: A Novel Mutation of SPG3A in a Large Family

Spastic paraplegia Type 3A is an autosomal-dominant pure or uncomplicated hereditary spastic paraplegia. It is caused by mutations in SPG3A, the only gene associated with this condition. We identified a novel mutation, c.1040T>C (p. M347T), in a family with axonal neuropathy in addition to spastic paraplegia. This expands the spectrum of neurologic complications associated with SPG3A and highlights the importance of long-term follow-up and neurological surveillance in this patient population.
Driven, distracted, or both? A performance-based ex-Gaussian analysis of individual differences in anxiety.

Since the inception of the empirical study of personality, and even before it, individual differences in anxiety and distress have been viewed as key predictors of behavioral performance. Yet such literatures have always entertained 2 perspectives, one contending that anxious individuals are "driven" and the other contending that anxious individuals are "distracted. " The present 3 studies (total N=289) sought to reconcile such discrepant views according to an ex-Gaussian parsing of reaction time performance tendencies in basic cognitive tasks. As hypothesized, a particular pattern marked by faster responding on the preponderance of trials (in terms of the ex-Gaussian μ parameter) in combination with slower responding on other trials (in terms of the ex-Gaussian τ parameter) was predictive of higher levels of anxiety. Implications for understanding neuroticism, distress, the anxiety-performance interface, and cognitive models of personality processes are discussed.
Components of the Residential Environment and Socio-Demographic Characteristics of the Elderly

Abstract This article aims to establish the components that best represent the different elements of the residential environment (home and building in which it is located, residential neighbourhood and neighbours) and analyse the relationships existing between the specific components that bring satisfaction and the characteristics of the residents aged 65 and over, with the hypothesis that better demographic and social conditions will have a positive influence on their residential perception. The data was drawn from a telephone survey, including objective and subjective items, that was conducted in 1998 with a representative sample of 1,148 people aged between 65 and 84 years who live in family housing in Madrid. A Factor Analysis was applied, giving 17 residential components, 10 related to the home/building element and 7 to the neighbourhood/neighbours element. This was followed by a Cluster Analysis of the 6 factors containing primary and specific residential satisfaction information, giving a variable of types of elderly people with 4 homogenous and self-excluding clusters. Subsequent statistical analyses revealed significant differences in the elderly population's residential satisfaction, associated to gender, age, perceived health status, social class, income and structure of the home in which they live, but not to their marital status, the number of people who live in their household nor the average age of the members of the household.
Biofilm‐Eradicating Properties of Quaternary Ammonium Amphiphiles: Simple Mimics of Antimicrobial Peptides

Bacterial biofilms are difficult to eradicate because of reduced antibiotic sensitivity and altered metabolic processes; thus, the development of new approaches to biofilm eradication is urgently needed. Antimicrobial peptides (AMPs) and quaternary ammonium cations (QACs) are distinct, yet well‐known, classes of antibacterial compounds. By mapping the general regions of charge and hydrophobicity of QACs onto AMP structures, we designed a small library of QACs to serve as simple AMP mimics. In order to explore the role that cationic charge plays in biofilm eradication, structures were varied with respect to cationic character, distribution of charge, and alkyl side chain. The reported compounds possess minimum biofilm eradication concentrations (MBEC) as low as 25 μM against Gram‐positive biofilms, making them the most active anti‐biofilm structures reported to date. These potent AMP mimics were synthesized in 1–2 steps and hint at the minimal structural requirements for biofilm destruction.
A Comparison of Two Group Classification Approaches to Fat-tailed and Skewed Data

The problem of two-group classification has implications in a number of fields, such as medicine, finance, and economics. This study aims to compare the methods of two-group classification. The minimum sum of deviations and linear programming model, linear discriminant analysis, quadratic discriminant analysis and logistic regression, multivariate analysis of variance (MANOVA) test-based classification and the unpooled T-square test-based classification methods, support vector machines and k-nearest neighbor methods, and combined classification method will be compared for data structures having fat-tail and/or skewness. The comparison has been carried out by using a simulation procedure designed for various stable distribution structures and sample sizes.
Flower-specific expression of the Agrobacterium tumefaciens isopentenyltransferase gene results in radial expansion of floral organs in Petunia hybrida.

SUMMARY Biotechnology has the potential to modify commercially important traits of crops, such as fruit size and stress tolerance. To date, the floricultural industry has not profited significantly from these possibilities to manipulate, for example, flower size. Cytokinins are known to be involved in many aspects of plant development, including cell division. Increasing the amount of cytokinins has the potential to increase the size of an organ, such as the flower or the fruit. The Agrobacterium tumefaciens cytokinin biosynthesis gene isopentenyltransferase (ipt) has been shown to increase cytokinin levels when introduced into plants. Moreover, it has a dramatic effect on the vegetative development of plants. The expression of the ipt gene under the control of the flower-specific Arabidopsis APETALA3 promoter in petunia (Petunia hybrida) increases the flower size dramatically, but with no effect on vegetative development. The resulting transgenic plants produced flowers with larger corolla diameter and greater total floral fresh weight. This strategy has the potential for use in the production of ornamental crops with large flowers and crop species with larger fruit.
CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES

Preimmunization of either guinea pigs or rabbits to bovine gamma globulin (BGG) prepares the animals for markedly enhanced antibody responses to 2,4-dinitrophenyl-BGG (DNP-BGG). This phenomenon is observed both in the primary anti-DNP antibody response to DNP-BGG and in the secondary anti-DNP antibody response to DNP-BGG in animals primed with DNP-ovalbumin (DNP-OVA). The BGG preimmunization is most effective if the antigen is administered as a complete Freund's adjuvant emulsion; in rabbits, a dose of 1 µg of BGG is more effective than a dose of 50 µg, whereas the reverse is true in guinea pigs. Transfusion of homologous anti-BGG sera fails to replace active immunization with BGG in the preparation of animals for these enhanced anti-DNP antibody responses. Both the immunoglobulin class and the average association constant for ε-DNP-L-lysine of the anti-DNP antibody produced in these enhanced responses is determined by the mode and time of immunization with haptenic conjugates and is not appreciably influenced by the nature of the carrier preimmunization. These studies indicate that the carrier specificity of hapten-specific anamnestic antibody responses is largely due to the interaction of two independent cell associated recognition units, one specialized for carrier and the other specific for haptenic determinants.
5,5′‐Methylenedisalicylic Acid (MDSA) Modulates SarA/MgrA Phosphorylation by Targeting Ser/Thr Phosphatase Stp1

SarA (staphylococcal accessory protein A), MgrA (MarR family of global transcriptional regulator A), and SarZ (a paralogue of SarA) play critical roles in modulating the virulence, drug resistance and autolysis of Staphylococcus aureus. Recently, eukaryotic‐like Ser/Thr kinase/phosphatases (Stk1/Stp1) were found to modulate phosphorylation of these transcriptional regulators as well as staphylococcal virulence. Importantly, an stp1‐deficient strain showed significant virulence reduction in mice, indicative of Stp1 as a potential drug target. Here, we report that MDSA, an inhibitor of MgrA, enhances phosphorylation of SarA/MgrA by inhibiting Stp1 in S. aureus. MDSA is a more‐potent inhibitor (IC50=9.68±0.52 μM) of Stp1 than commonly used phosphatase inhibitors. We anticipate that MDSA could be a lead compound to develop new approaches for reducing staph virulence by targeting Stp1.
Two‐Step Protein Labeling by Using Lipoic Acid Ligase with Norbornene Substrates and Subsequent Inverse‐Electron Demand Diels–Alder Reaction

Inverse‐electron‐demand Diels–Alder cycloaddition (DAinv) between strained alkenes and tetrazines is a highly bio‐orthogonal reaction that has been applied in the specific labeling of biomolecules. In this work we present a two‐step labeling protocol for the site‐specific labeling of proteins based on attachment of a highly stable norbornene derivative to a specific peptide sequence by using a mutant of the enzyme lipoic acid ligase A (LplAW37V), followed by the covalent attachment of tetrazine‐modified fluorophores to the norbornene moiety through the bio‐orthogonal DAinv . We investigated 15 different norbornene derivatives for their selective enzymatic attachment to a 13‐residue lipoic acid acceptor peptide (LAP) by using a standardized HPLC protocol. Finally, we used this two‐step labeling strategy to label proteins in cell lysates in a site‐specific manner and performed cell‐surface labeling on living cells.
Compassion Satisfaction, Burnout, and Secondary Traumatic Stress in Heart and Vascular Nurses

Objectives for this project were to determine the prevalence of compassion satisfaction (CS), burnout, and secondary traumatic stress (STS) in heart and vascular nurses to confirm whether differences exist between intensive care and intermediate care nurses. The Professional Quality of Life Scale Compassion Satisfaction and Compassion Fatigue: Version 5 developed by Stamm (2009) was used. Results showed that nurses who work in the heart and vascular intermediate care unit had average to high scores of CS, low to average levels of burnout, and low to average levels of STS. Nurses who work in the heart and vascular intensive care unit had average to high levels of CS, low to average levels of burnout, and low to average levels of STS. These findings suggest that leadership should be aware of the prevalence of STS and burnout in heart and vascular nurses. Raising awareness of STS and burnout in intensive care and intermediate care nurses can help in targeting more specific strategies that may prevent the onset of developing these symptoms.
The effectiveness of standard pediatric daycare surgery discharge communication: A quality improvement gap analysis

Abstract The aim of this manuscript is to identify for the reader how communication gaps during handovers from hospital personnel to parents after pediatric day surgery can endanger patient safety. Variables analyzed include parent-reported quality of communication and hospital safety culture, medication safety, surgical wound care, and nausea and vomiting knowledge. Data are obtained via a 21-item questionnaire. The reader is informed of a technique for communication quality measurement that combines naturalistic observation of the discharge process, a theoretical model of discharge communication and safety domain modeling and analysis. The reader is informed via the discharge communication and safety domain model of the areas posing the most risk to patient safety in the post-discharge period.
M-Estimation in the partially linear model with Bernstein polynomials under shape constrains

ABSTRACT We develope an M-estimator for partially linear models in which the nonparametric component is subject to various shape constraints. Bernstein polynomials are used to approximate the unknown nonparametric function, and shape constraints are imposed on the coefficients. Asymptotic normality of regression parameters and the optimal rate of convergence of the shape-restricted nonparametric function estimator are established under very mild conditions. Some simulation studies and a real data analysis are conducted to evaluate the finite sample performance of the proposed method.
Do the Brain Perfusion SPECT Findings Reflect the Outcome of Rehabilitation in Stroke Patients?

The purpose of this study is to investigate the association between brain perfusion single-photon emission computed tomography (SPECT) changes and the observed improvement in the clinical parameters, and therefore evaluate whether or not SPECT can predict the outcome of the rehabilitation of patients. Nineteen stroke patients were included in the prospective study. Patients were hospitalized within the first month after their stroke and were in the rehabilitation program for 2 months. A clinical evaluation and a brain perfusion SPECT was conducted during the admission, and on the completion of the rehabilitation program. The outcome measurements included Brunnstrom stage, Modified Ashworth Scale, functional independence measurement, functional ambulation categories, and mini-mental state examination. The brain perfusion SPECT was conducted by the evaluation of the regional cerebral blood flow on 12 different regions on both of the hemispheres. The comparison of the first and the third month clinical scores showed a statistically significant difference. However, the brain perfusion SPECT values' first and third month comparisons showed no difference on any of the measured parameters. Furthermore, there was no correlation between the clinical scores and SPECT values, for both the first month measures and the third month measures. The observed clinical improvement observed during the rehabilitation process of the stroke patients has no association to brain perfusion SPECT evaluations. Brain perfusion SPECT could not be a very good predictor for measuring clinical outcomes for stroke patients.
A Case of 17 Years Old Male with Short Stature and Delayed Adolescence due to Distorted Body Image

We report a 17 yr old male with short stature and delayed adolescence due to distorted body image. He was admitted to our hospital because of growth failure at the age of 15 yr 7 mo. His growth had been normal, but after entering a junior high school, he began to take an interest in physical exercise and protein diet. His endocrine data and psychopathological profiles such as eating, training pattern and extreme diet and failure to make expected growth and puberty were compatible with DSM-IV and ICD-10 criteria for the diagnosis of anorexia nervosa (AN). The symptoms of male AN are different from female AN and it is difficult to diagnose. We emphasize the necessity of considering AN in the differential diagnosis of growth retardation and delayed adolescence in young males.