Miracle-dz/UniFAR-FTUs huggingface dataset:
Variation of Anaeromyxobacter community structure and abundance in paddy soil slurry over flooding time

Cultivation-independent techniques like PCR-amplified restriction fragment length polymorphism (PCR-RFLP) of 16S rRNA genes and real-time PCR were applied to assess the abundance, diversity and phylogenetic composition of Anaeromyxobacter communities over time in flooded, unplanted paddy soil slurries. Six Anaeromyxobacter communities were sampled from anoxic slurries at 1 h, and 1, 5, 10, 20 and 30 days while the Fe (II) concentrations were measured also. Bacterial Genomic DNA was extracted and PCR-amplified to obtain 16S rDNA fragments of Anaeromyxobacter which were cloned to construct 6 16s rDNA libraries. Eventually 10 major Anaeromyxobacter types were identified by RFLP fingerprintings. Results showed that the optimal increasing phase of Fe (II) was from 1h to nearly 10 days, being correspond with the growth phase of the abundance of Anaeromyxobacter. The highest diversity appeared in slurry at 30 days and the lowest was found at 30 days. Jackknife Environment Clusters by UniFrac showed that phylogenetic compositions of Anaeromyxobacter communities in slurries at 10 and 20 days were the most similar. By evolutionary distance analysis, our 10 major Anaeromyxobacter types were diverged into Group 1 and 2 in phylogenetic tree, while Group 1 was the exclusive collection of clones from our experiment. Major type P1 was present in all slurries abundantly and P9 only existed in slurry at 5 days. The abundance of Anaeromyxobacter spp. , calculated as its proportion of 16S rDNA copies to the value of total Bacteria, was from 0.242% at 1 h to 5.135% at 10 days. We demonstrated that flooding time led to successional dynamics of major types and variable abundance of Anaeromyxobacter community. Flooding time also influenced the diversity of Anaeromyxobacter community on some extent. Canonical correspondence analysis (CCA) revealed that Anaeromyxobacteria spp. abundance had interrelation with Fe (II) content and the influenced the distribution of the slurries in the Biplot. Moreover, our study provides valuable information for the further isolation of Anaeromyxobacter strains from paddies. Key words: Anaeromyxobacter; paddy soil, community structure, abundance, PCR-RFLP, flooding time.
THE RESPONSE OF CANDIDA ALBICANS TO SIMULATED MICROGRAVITY

Microgravity presents a unique environmental stress on microorganisms that have evolved under normal gravity. Notably, an increase of virulence has been observed in several prokaryotes upon exposure to microgravity. Modeling the microgravity environment, using rotating suspension culture bioreactors, provides insights into cellular physiology in a ground-based model. Studying Candida albicans, a common eukaryotic opportunistic pathogen, allows examination of basic eukaryotic cell biology, as well as yeast pathogenicity in response to this extreme environment. The morphology of C. albicans contributes to pathogenicity—yeast forms are necessary for dissemination via the blood, hyphal forms are necessary for tissue invasion, while biofilm communities are important for environmental protection, including resistance to antimicrobials, such as Amphotericin B (Bastidas, Heitman, 2009; Odds, 1994; Ramage et al., 2009). Previous studies from our laboratory have shown that C. albicans experiences increased filamentation when exposed to simulated microgravity (SMG). Consistent with the morphological changes observed in conditions of SMG, increased expression of filamentation-specific genes, including Hwp1 was been verified by qRT-PCR (Altenburg et al., 2008). The SMG-induced genotypic and phenotypic changes that occur in C. albicans may predict a gravity-sensitive molecular pathway contributing to heightened pathogenicity.
HOW IMPORTANT IS THE THERAPEUTIC ALLIANCE IN TREATING OBSESSIVE-COMPULSIVE DISORDER WITH EXPOSURE AND RESPONSE PREVENTION? AN EMPIRICAL REPORT.

Objective Substantial research has established exposure and response prevention (EX/RP) as an effective treatment for obsessive-compulsive disorder (OCD). Yet, the role of the therapeutic alliance as a factor in EX/RP remains a relatively understudied area. We sought to investigate this issue and explore which aspects of the alliance matter most to treatment outcome. Method Data came from 37 adult OCD patients who completed 17 sessions of manualized EX/RP as part of a randomized controlled trial of SRI augmentation. Patients rated the therapeutic alliance at the third therapy session using the Working Alliance Inventory-Short form (WAI-SF), which includes three subscales to rate alliance dimensions (Goal, Task, and Bond) as well as a total score reflecting the overall strength of the working alliance. OCD symptoms were rated at baseline and post-treatment using the Yale-Brown Obsessive-Compulsive Scale (YBOCS). In addition, therapists rated the degree to which patients adhered to between session EX/RP assignments. Results Adjusting for baseline severity, total score on the WAI-SF did not significantly predict post-treatment YBOCS. Follow-up analyses revealed that higher scores on the Task subscale were significantly associated with lower post-treatment severity. Scores on the Task subscale also predicted degree of patient adherence to between session EX/RP assignments, which mediated the relationship between task alliance and treatment outcome. Conclusions Although overall ratings of the quality of the therapeutic alliance were not related to EX/RP outcomes, the degree to which patients and therapists allied on the tasks of therapy did predict outcomes, suggesting that this particular aspect of the therapeutic alliance matters most to EX/RP outcomes. Better agreement on the tasks of therapy also related to better EX/RP adherence, which mediated treatment outcome. Limitations and clinical implications are discussed.
Ocena sprawności fizycznej dzieci i młodzieży z terenów wiejskich na przykładzie uczniów Zespołu Szkół w Ołdakach

Karolina Dąbrowska1, Mateusz Cybulski2, Anna Konopka3, Elżbieta Krajewska-Kułak2 © Pediatr Med Rodz 2017, 13 (4), p. 527–539 © Medical Communications Sp. z o.o. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (CC BY-NC-ND). Reproduction is permitted for personal, educational, non-commercial use, provided that the original article is in whole, unmodified, and properly cited. DOI: 10.15557/PiMR.2017. 0056 Received: 28.06. 2017 Accepted: 18.09. 2017 Published: 29.12. 2017 Karolina Dąbrowska, Mateusz Cybulski, Anna Konopka, Elżbieta Krajewska-Kułak
CONSIDERACIONES SOBRE LA INTERRELACION ENTRE LA GESTION DEL CONOCIMIENTO Y LA GESTION DE LA INNOVACION

Se presenta un conjunto de consideraciones que analizan la interrelacion existente entre la Gestion del Conocimiento y la Gestion de la Innovacion, dos areas de investigacion con una notable importancia a partir del aumento del impacto de los activos intangibles, del capital intelectual, de la innovacion y de un comportamiento emprendedor en la Gestion Empresarial de la actualidad; esta interrelacion se expresa en la funcion de I+D y en su principal unidad de analisis: el proyecto. En el trabajo tambien se hace referencia a los cinco grupos de practicas de la Gestion del Conocimiento dentro de los procesos de innovacion, asi como a dos herramientas decisivas en la gestion del conocimiento en el marco de los proyectos de I+D e innovacion tecnologica: la vigilancia tecnologica y el benchmarking. Se dan algunos ejemplos de la aplicacion de esta interrelacion en la Estacion Experimental de Pastos y Forrajes "Indio Hatuey".
The Impact of Cannabidiol on Psychiatric and Medical Conditions

Cannabidiol (CBD) is a substance chemically derived from Cannabis sativa and discussed to be non-psychoactive. According to the FDA, marijuana is classified as a schedule I substance; however, hemp which is defined as extracts from marijuana including cannabinoids containing less than 0.3% tetrahydrocannabinol (THC), is excluded from that controlled substance act and available at local convenience stores in the US as it is seen as an herbal supplement. CBD is purported to be used for various medical and psychiatric conditions: depression, anxiety, post-traumatic stress disorder, Alzheimer’s or other cognitive illnesses as well as pain. There is also a new trend to use CBD for the treatment of opioid use disorder. The one CBD product on the market that is FDA approved for the treatment of childhood epilepsy forms Dravet and Lennox-Gastaut syndromes is available under the name Epidiolex. There is a significant difference between this medication and the over-the-counter CBD products that contain very inconsistent strengths of CBD, if they contain it at all, and vary in percentage even from sample to sample. Frequently the so-called CBD products are not containing any CBD at all, but mostly containing THC. This article is a systematic review of literature reviewing the available clinical data on CBD, for use in various medical and psychiatric conditions with focus on a review of the pharmacology and toxicity. Resources used were ORVID, PubMed, MEDLINE, PsychINFO, EMBASE with keywords CBD, cannabidiol, hemp and cannabinoids.
Morphology and sexual selection in the barn swallow Hirundo rustica in Chernobyl, Ukraine

Secondary sexual characters are large structures of great intricacy of design, and there are many ways in which their development can be disrupted. Both somatic and germ-line mutations affecting the expression of secondary sexual characters are likely to be biased and most frequently reduce performance because of the great complexity of characters. I investigated the degree of fluctuating asymmetry and the incidence of aberrant secondary sexual characters in the monogamous barn swallow Hirundo rustica in two areas in Ukraine, one near Chernobyl, and the other near Kanev. I predicted that developmental disorders should be more frequent in a recent sample of birds from the Chernobyl area than in samples from other areas, and than in museum samples pre-dating the 1986 contamination event. There were apparently no consistent effects of area or time period on the morphology of barn swallows. The level of fluctuating asymmetry in male tail length was considerably elevated in the Chernobyl area in the recent sample, but not in females, and three other morphological variables were unaffected in both sexes. Males with high degrees of asymmetry in their tails bred later than symmetric males. The incidence of aberrant feather morphology in the Chernobyl area was higher for tail ornaments of males than for other morphological characters in males, and than for the homologous character of females, and there were no cases of aberrant feathers in the control area or in the museum samples. Males with aberrant tail feathers bred later than other males.
Twisted compactifications of 3d N = 4 theories and conformal blocks

Three-dimensional N = 4 supersymmetric quantum field theories admit two topological twists, the Rozansky-Witten twist and its mirror. Either twist can be used to define a supersymmetric compactification on a Riemann surface and a corre- sponding space of supersymmetric ground states. These spaces of ground states can play an interesting role in the Geometric Langlands program. We propose a description of these spaces as conformal blocks for certain non-unitary Vertex Operator Algebras and test our conjecture in some important examples. The two VOAs can be constructed respectively from a UV Lagrangian description of the N = 4 theory or of its mirror. We further conjecture that the VOAs associated to an N = 4 SQFT inherit properties of the theory which only emerge in the IR, such as enhanced global symmetries. Thus knowledge of the VOAs should allow one to compute the spaces of supersymmetric ground states for a theory coupled to supersymmetric background connections for the full symmetry group of the IR SCFT. In particular, we propose a conformal field theory description of the spaces of ground states for the T[SU(N)] theories. These theories play a role of S-duality kernel in maximally supersymmetric SU(N) gauge theory and thus the corresponding spaces of supersymmetric ground states should provide a kernel for the Geometric Langlands duality for special unitary groups.
Readministration of Cancer Drugs in a Patient with Chemorefractory Metastatic Colorectal Cancer

A 63-year-old woman was admitted to our institution for severe pain in her right lower abdomen caused by the perforation of cecal cancer. She underwent emergency surgery, from which she was diagnosed with cecal carcinoma with liver, lung, and lymph node metastases. As she was taking aspirin to prevent cerebral infarction, anti-vascular endothelial growth factor (receptor) antibody and regorafenib therapy were not used. Thus, we started a modified FOLFOX 6+cetuximab regimen. This first-line treatment initially achieved a partial response (PR), but she then developed progressive disease (PD) after 14 months. We changed the regimen to FOLFIRI, followed by trifluridine/tipiracil, but her progression-free survival periods were 2.7 months and 1 month, respectively. Although we cycled through the available array of standard cancer drugs, the patient showed a good performance status, and some benefit from treatment still seemed plausible. We readministered the 5-fluorouracil oral preparation S-1, which maintained stable disease (SD) for 7 months. After PD emerged, we readministered the anti-epidermal growth factor receptor (EGFR) antibody panitumumab for 7.5 months of SD. Finally, 39 months after her diagnosis, she died from rapidly progressing disease. However, her relatively long survival implies that readministering drugs similar to those used in previous regimens might benefit patients with metastatic colorectal cancer.
Optimization of conditions for production of neutral and alkaline protease from species of Bacillus and Pseudomonas

Two mesophilic bacterial isolates, S4 and S22 producing extracellular protease were Isolated from soil and identified as Bacillus sp. and Pseudomonas sp. Isolate S4 produced neutral protease, whereas, isolate S22 producedalkaline protease. Optimization studies showed wheat bran and soybean meal to be the best carbon and nitrogen source for production of proteases. Alkaline protease from Pseudomonas sp. S22 was partially purified by ammonium sulfate precipitation and gel filtration.The molecular mass of the enzyme determined by SDS-PAGE was approximately 46 kD.
Establishing links between alcohol intake, cognitive functions and type 2 diabetes

In the present report, we studied the associations that might exist between alcohol consumption, cognitive func- tions and diabetic pathology in patients with type 2 diabetes (T2D). The alcohol intake of 219 patients diagnosed with diabetes was classified into 6 groups: nondrinkers, 0.1-9.9, 10.0-14.9, 15.0-29.9, 30.0-49.9 and ≥ 50.0, according to the total amount (grams/day) of alcohol consumption. Our results mainly confirm that moderate alcohol consumption can reduce some of the neuropathological aspects of T2D, as demonstrated by the decrease in glycemic levels in patients that con- sumed higher levels of alcohol (30.0-49.9 g/day), when compared to non-drinkers (p=0.04) or groups in which individuals consumed 0.1-9.9 g/day (p=0.01) and 10.0-14.9 g/day (p=0.02). Regarding the results of cognitive testing, we noticed a significant increase in the values of the MMSE score a lower dose of alcohol intake (0.1-9.9 g/day) was compared with higher doses: 30.0-49.9 g/day (p=0.008) and ≥ 50.0 g/day (p=0.047).
Different apparent CO2 compensation points in nitrate- and ammonium-grown Phaseolus vulgaris and the relationship to non-photorespiratory CO2 evolution

The classical theory of the relationship between gas fluxes and photosynthetic electron fluxes was extended by two additional terms: J L describing flux to electron sinks other than the Calvin cycle, and R L accounting for light-induced changes in non-photorespiratory CO 2 evolution. R L comprises two main components, R r resulting from light-induced decrease in tricarboxylic acid activity, and R S related to extra CO 2 evolution resulting from citrate-to-2-oxoglutarate conversion for N-assimilation in NO 3 - grown leaves. This extended theory was applied to two experiments. First, A-C i curves (dependence of CO 2 flux on stomatal CO 2 concentration) revealed a higher apparent CO 2 compensation point (Γ* app ) in NO 3 - -grown plants than in NH 4 + -grown plants. Secondly, photosynthetic electron fluxes at different light intensities were determined by means of the Genty parameter of chlorophyll fluorescence and compared with those calculated from measured CO 2 uptake. Curve-fitting based on the extended theory provided a coincidence of these two measurements and resulted in higher R S in NO 3 - -grown than in NH4 + -grown plants. This difference in R S (about 15% of the CO 2 flux bound by carboxylation) is the same as that obtained from the analysis of Γ* app . Further, the analysis suggests that J L related to the extra electron flux used for N-assimilation in NO 3 - -grown plants is diverted to other sinks in NH4 + -grown plants. SHAM decreased photosynthetic electron flow and O 2 evolution in NH 4 + -grown plants, anti-mycin A in NO 3 - -grown plants. The effect of oligomycin was small. The results are discussed in terms of different mechanisms of chloroplast/mitochondrion interaction in NO 3 - - and NH 4 + -grown plants, their effects on non-photorespiratory CO 2 evolution and on Γ* app .
The genome of the butternut canker pathogen, Ophiognomonia clavigignenti-juglandacearum shows an elevated number of genes associated with secondary metabolism and protection from host resistance responses

Ophiognomonia clavigignenti-juglandacearum (Oc-j) is a plant pathogenic fungus that causes canker and branch dieback diseases in the hardwood tree butternut, Juglans cinerea. Oc-j is a member of the order of Diaporthales, which includes many other plant pathogenic species, several of which also infect hardwood tree species. In this study, we sequenced the genome of Oc-j and achieved a high-quality assembly and delineated its phylogeny within the Diaporthales order using a genome-wide multi-gene approach. We also further examined multiple gene families that might be involved in plant pathogenicity and degradation of complex biomass, which are relevant to a pathogenic life-style in a tree host. We found that the Oc-j genome contains a greater number of genes in these gene families compared to other species in the Diaporthales. These gene families include secreted CAZymes, kinases, cytochrome P450, efflux pumps, and secondary metabolism gene clusters. The large numbers of these genes provide Oc-j with an arsenal to cope with the specific ecological niche as a pathogen of the butternut tree.
Hemocyanin a Most Likely Inducer of Black Spots in Kuruma Prawn Penaeus japonicus During Storage

Hemocyanin (Hc) from plasma of kuruma prawn was characterized as a potent inducer of black spots during storage. An oxygen transporter, hemocyanin was converted into phenoloxidase (PO)-like enzyme (HdPO) with SDS treatment as well as prophenoloxidase isolated from hemocytes. Both enzymes showed similar biochemical properties. The Isolated PO, however, was so unstable that its activity was drastically reduced when frozen and thawed at -25 °C for less than a week, while HdPO completely retained its activity for more than a month, suggesting that HdPO is a key factor in black spot development in freeze-thawed prawn. Le developpement de taches noires sur les crevettes Penaeus japonicus resulte de l'oxydation enzymatique des phenols et de leur derives par une phenoloxydase. Dans cette etude, l'hemocyanine du plasma des crevettes est caracterisee en tant qu'inducteur potentiel de ce phenomene. Les resultats indiquent que le developpement des taches noires apres un entreposage en congelation serait attribue a une phenoloxydase derivee de l'hemocyanine.
Intimate partner violence among postpartum women at a teaching hospital in Nigeria’s Federal Capital City: pattern and materno-fetal outcomes

Background: Intimate partner violence is an important public health and human rights issue. Previous studies have considered intimate partner violence in pregnancy mainly among pregnant women attending antenatal clinics thereby missing out a few who may encounter this problem in late pregnancy or just before delivery. This study had the objective of ascertaining the prevalence, pattern of intimate partner violence, and associated materno-fetal outcomes. Method: This was a cross-sectional study conducted between January 2017 and June 2017 among postpartum mothers at a Nigerian Teaching Hospital just before being discharged home. The abuse assessment score was adapted and used to interview women regarding possible intimate partner violence experiences within the past 1 year and during the pregnancy after obtaining written consent. Results: Out of 349 postpartum women interviewed, 102/349 (29.2%) experienced intimate partner violence in the past 1 year, while 18/349 (5.2%) of intimate partner violence occurred in the index pregnancy. Sexual partners were the main perpetuators of intimate partner violence, 67/102 (65.7%), while 35/102 (34.3%) were by someone else other than their sexual partners. Among those abused in the current pregnancy, 10/18 (55.6%) were abused once and the remaining 8/18 (44.4%) were abused more than once. Intimate partner violence was associated with higher chances of cesarean section (p = 0.001), increased risk of lesser birth weight babies (p = 0.014), and maternal complications in pregnancy (p = 0.030). Conclusion: The prevalence of intimate partner violence in pregnancy in Abuja is high with associated poor materno-fetal outcomes. Enforcing existing legislations and screening for intimate partner violence during routine antenatal care may help reduce its prevalence and ensure a positive pregnancy experience for Nigerian women.
Rapid, Low-Cost Dielectrophoretic Diagnosis of Bladder Cancer in a Clinical Setting

Bladder cancer is the 9th most common cancer worldwide. Diagnosing bladder cancer typically involves highly invasive cystoscopy, with followup monitored using uteroscopy. Molecular methods have been developed as an adjunct to this, but tend to be expensive or require expert operator input. Here we present a study of the use of dielectrophoresis (DEP) of voided cells from eight cancer-presenting patients and eight healthy controls as an alternative low-cost and operator-independent method of bladder cancer detection. This study suggests that there are statistically significant differences ( $p=0.034$ ) between characteristics of the DEP spectrum of clinical samples, and that using this marker we were able to obtain sensitivity of 75% and specificity of 88%, in line with many molecular methods; exclusion of samples where a DEP spectrum is not present (due to low cell counts) increased sensitivity to 100%, showing this can be improved by increasing the cell collection rate. As samples were analyzed a day after collection, we suggest that the method may be amenable to a centralized mail-in analysis service.