Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of fertilization outcome between microdrop and open insemination methods in non-male factor IVF patients

Abstract Both microdrop and open methods are commonly used for in vitro fertilization (IVF) protocols for embryo culture as well as oocyte insemination. However, few comparative studies evaluating the microdrop or open method of insemination on the fertilization outcome and subsequent embryo development have been performed. A randomized study was conducted to compare microdrop and open fertilization with respect to fertilization rate and embryo development among non-male factor patients undergoing in vitro fertilization and embryo transfer (IVF-ET). The results presented in this study demonstrate that the fertilization failure rate [total fertilization failure rate (TFF) plus low fertilization rate (<25% oocytes fertilized)] in the microdrop insemination group was higher than in the open insemination group (11.9% versus 3.3%, p < 0.001), while the good quality embryo rate and pregnancy rate did not differ significantly between the groups. As a highly complicated process involving many extrinsic and intrinsic factors, further studies are needed to confirm the effects of these insemination methods on the rate of fertilization failure.
Al2O3 doped apatite-wollastonite containing glass ceramic provokes osteogenic differentiation of marrow stromal stem cells.

Fresh marrow cells were obtained from femora of Fischer rats and cultured in a medium containing 15% fetal calf serum (FCS) until confluence. After trypsinization, cells were subcultured at a cell density of 100 x 10(3)/35-mm well in the presence of FCS, beta-glycerophosphate, and ascorbic acid phosphate on four different culture substrata. The period of subculture was 2 weeks; the substrata used were the culture dish, apatite-wollastonite containing glass ceramic (AW), hydroxyapatite coated AW (HA/AW), and Al2O3 doped AW (Al/AW). The HA coating was attained by the incubation of AW in simulated physiological solution. The glass matrix of AW and HA/AW contained MgO, CaO, P2O5, and SiO2; Al/AW contained Al2O3 in addition to these components. The subculture on Al/AW substratum showed many alkaline phosphatase (ALP) positive nodules and the highest ALP activity. On a Northern blot analysis the housekeeping gene of beta-actin mRNA was evenly detected from the cells cultured on all substrata; however, bone-specific osteocalcin mRNA was only detected from the cells on Al/AW. These results indicate that Al/AW provokes the osteoblastic differentiation of marrow stromal stem cells.
The consequences of trade union power erosion

The micro- and macroeconomic effects of the declining power of trade unions have been hotly debated by economists and policymakers. Nevertheless, the empirical evidence shows that the impact of the decline on economic aggregates and firm performance is not an overwhelming cause for concern. However, the association of declining union power with rising earnings inequality and a loss of direct communication between workers and firms is potentially more worrisome. This in turn raises the questions of how supportive contemporary unionism is of wage solidarity, and whether the depiction of the nonunion workplace as an authoritarian “bleak house” is more caricature than reality.
Separating induction Motor Current Signature for stator winding faults from that due to supply voltage unbalances

Statistical spreads of the surveys suggest that stator winding faults are one of the most prevailing faults in induction motor. Most of the methods for stator winding inter-turn fault diagnosis are based on Motor Current Signature Analysis (MCSA) combined with signal-and-data processing tools. Fault diagnosis using MCSA becomes more challenging when stator current signatures due to winding short circuit fault and supply voltage unbalance appear identical. The present paper proposes a method through analysis of Park's Vector Modulus (PVM) to discriminate stator winding inter-turn fault cases, from supply voltage unbalance conditions where both cases exhibit apparently similar kind of current signatures. A series of experiments have been performed on a motor with different degrees of stator winding inter-turn faults. The same motor under healthy condition was also tested while operating under unbalanced supply voltages that caused similar current unbalances as in the case of inter-turn short circuit faults. This work aims at identification of the motor voltage unbalance conditions separately from inter-turn fault cases through detection of high frequency signals present in different PVM profiles. Signal processing tools such as Fast Fourier Transform (FFT), Discrete Wavelet Transform (DWT) and Power Spectral Density (PSD) calculation have been employed to discriminate inter-turn short circuit faults from supply voltage unbalance conditions of the motor at different load levels. Entire analysis presented in this paper is based on experimentally obtained motor current signatures.
Free Breathing Real-Time Cardiac Cine Imaging With Improved Spatial Resolution at 3 T

ObjectivesThe aim of this study was to evaluate free-breathing single-shot real-time cine imaging for functional cardiac imaging at 3 T with increased spatial resolution. Special emphasis of this study was placed on the influence of parallel imaging techniques. Materials and MethodsGradient echo phantom images were acquired with GRAPPA and modified SENSE reconstruction using both integrated and separate reference scans as well as TGRAPPA and TSENSE. In vivo measurements were performed for GRAPPA reconstruction with an integrated and a separate reference scan, as well as TGRAPPA using balanced steady-state free precession protocols. Three clinical protocols, rtLRInt (Tres = 51.3 milliseconds; voxel, 2.5 × 5.0 × 10 mm3), rtMRSep (Tres = 48.8 milliseconds; voxel, 1.9 × 3.1 × 10 mm3), and rtHRSep (Tres = 48.3 milliseconds; voxel, 1.6 × 2.6 × 10 mm3), were investigated on 20 volunteers using GRAPPA reconstruction with internal as well as separate reference scans. End-diastolic volume, end-systolic volume, ejection fraction, peak ejection rate, peak filling rate, and myocardial mass were evaluated for the left ventricle and compared with an electrocardiogram-triggered segmented readout cine protocol used as standard of reference. All studies were performed at 3 T. ResultsPhantom and in vivo data demonstrate that the combination of GRAPPA reconstruction with a separate reference scan provides an optimal compromise of image quality as well as spatial and temporal resolution. Functional values (P values) for the standard of reference, rtLRInt, rtMRSep, and rtHRSep end-diastolic volume were 141 ± 24 mL, 138 ± 21 mL, 138 ± 19 mL, and 128 ± 33 mL, respectively (P = 0.7, 0.7, 0.4); end-systolic volume, 55 ± 15 mL, 61 ± 14 mL, 58 ± 12 mL, and 55 ± 20 mL, respectively (P = 0.23, 0.43, 0.62); ejection fraction, 61% ± 5%, 57% ± 5%, 58% ± 4%, and 56% ± 8%, respectively (P = 0.01, 0.11, 0.06); peak ejection rate, 481 ± 73 mL/s, 425 ± 62 mL/s, 434 ± 67 mL/s, and 381 ± 86 mL/s, respectively (P = 0.03, 0.04, 0.01); peak filling rate, 555 ± 80 mL/s, 480 ± 70 mL/s, 500 ± 70 mL/s, and 438 ± 108 mL/s, respectively (P= 0.007, 0.05, 0.004); and myocardial mass, 137 ± 26 g, 141 ± 25 g, 141 ± 23 g, and 130 ± 31 g, respectively (P = 0.62, 0.54, 0.99). ConclusionsUsing a separate reference scan and high acceleration factors up to R = 6, single-shot real-time cardiac imaging offers adequate temporal and spatial resolution for accurate assessment of global left ventricular function in free breathing with short examination times.
Search for anomalously heavy hydrogen in deep sea water at 4000 m.

A search was made with a sea water sample taken at a depth of 4000 m for anomalously heavy hydrogen dating from the early Universe. A technique of accelerator mass spectrometry involving a time-of-flight spectrometer was used. A new upper limit for the concentration of heavy particles in hydrogen is set around 4[times]10[sup [minus]17] in the mass range of 5--1600 u at a 95% confidence level.
SPECKLE INTERFEROMETRY AT THE BLANCO AND SOAR TELESCOPES IN 2008 AND 2009

The results of speckle interferometric measurements of binary and multiple stars conducted in 2008 and 2009 at the Blanco and SOAR 4 m telescopes in Chile are presented. A total of 1898 measurements of 1189 resolved pairs or sub-systems and 394 observations of 285 un-resolved targets are listed. We resolved for the first time 48 new pairs, 21 of which are new sub-systems in close visual multiple stars. Typical internal measurement precision is 0.3 mas in both coordinates, typical companion detection capability is Δm ∼ 4.2 at 0. ″15 separation. These data were obtained with a new electron-multiplication CCD camera; data processing is described in detail, including estimation of magnitude difference, observational errors, detection limits, and analysis of artifacts. We comment on some newly discovered pairs and objects of special interest.
Brain biopsy in children with primary small‐vessel central nervous system vasculitis

Primary angiitis of the central nervous system in childhood (cPACNS) is an immune‐mediated inflammatory process directed toward blood vessels in the central nervous system. It has been associated with variable clinical and radiological presentations, and devastating consequences without treatment. Brain biopsy is required for definitive diagnosis. The objective of this study was to characterize the clinical and histopathological features of brain biopsies in small‐vessel cPACNS (SVcPACNS).
Limited development capacity of the earliest embryonic murine thymus

Previous studies have demonstrated that murine thymus separates from the pharynx during 11.5–12 days of gestation, and that the proliferation of thymic cells starts at this age. We characterized embryonic day 12 thymus in terms of the surface phenotype of the thymus cells, the function of the lobe in supporting T cell development in organ culture, and the precursor activity of the thymus cells in a mixed culture with deoxyguanosine‐treated lobes.
Exposure to a high concentration of mite allergen in early infancy is a risk factor for developing atopic dermatitis: A 3‐year follow‐up study

The cause of allergy is multi‐factorial, and the development of an allergic disease seems to be the result of an interaction between genetic and environmental factors. The goal for preventing the development of allergic diseases is to avoid sensitization to allergens. The aim of this work was to study whether or not exposure to environmental allergens early in infancy would influence the occurence of various allergic diseases in later life. On an annual basis, a total of 931 healthy newborns were followed‐up until they reached 3 years of age. The occurence of allergic diseases was recorded by trained medical students during visits. Measurement of Dermatophagoides pteronyssinus (Der p 1) concentration in house dust was performed when each baby was 18 and 36 months old. Total and specific immunoglobulin E (IgE) antibodies against Der p 1, cow's milk, and egg white were evaluated at birth and at 18 months of age. The following results were obtained: at 3 years of age, 10.4% had bronchial asthma (BA), 21.4% atopic dermatitis (AD), 7.0% urticaria, and 46.8% had experienced wheezing; higher family allergy scores led to a higher incidence of AD (p=0.0012); exposure to a mite allergen concentration of 1 µg/g of dust may be associated with a higher incidence of AD (p=0.0156); the presence of Der p 1 IgE antibody at 18 months of age was associated with a higher incidence of BA (p=0.0001); and children sensitized to egg whites at 18 months of age had an increased risk of developing AD at 3 years of age (p=0.0187). Hence, early exposure to mite allergen is a risk factor for the development of atopic dermatitis, but seems not to be related to the development of bronchial asthma. Early sensitization to egg whites increases the risk of developing AD. The early detection of serum Der p 1 IgE antibody is associated with a higher incidence of bronchial asthma.
A solution processed top emission OLED with transparent carbon nanotube electrodes

Top emission organic light emitting diodes (OLEDs) with carbon nanotubes (CNTs) as top electrodes were fabricated and characterized. Devices were fabricated on glass substrates with evaporated bottom Al/LiF cathodes, a spin coated organic emissive layer and a PEDOT-PSS hole injection layer. Transparent thin CNT films were deposited on top of the emission layer to form the anode by micro-contact printing with a polydimethylsiloxane stamp. A very good device performance was obtained, with a peak luminance of 3588 cd m − 2 and a maximum current efficiency of 1.24 cd A − 1. This work shows the possibility of using CNTs as transparent electrodes to replace ITO in organic semiconductor devices. Furthermore, the top emission nature of such devices offers a broader range of applications of CNTs on any type of substrate. By combining with solution processed organic materials, it is anticipated that lower cost fabrication will be possible through roll-to-roll manufacture.
The Effects of Retinoic Acid on Ciliary Function of Regenerated Sinus Mucosa

Background Retinoic acid (RA) has been shown to enhance ciliary ultrastructure in regenerated sinus mucosa compared with controls. However, the functional status of the regenerated cilia has not been studied. Our objective was to evaluate the ciliary beat frequency (CBF) of regenerated sinus mucosa treated with topical RA in a rabbit model. Methods Twelve rabbits underwent bilateral surgical stripping of the maxillary sinuses, followed by treatment with RA gel in the right side and an inert gel control in the left side. The rabbits were then killed at either 2 or 4 weeks, and CBF analysis of the regenerated mucosa was performed. Three unoperated rabbits were used to establish normative CBF data. Results Functional cilia were recovered from 11/12 RA-treated sinuses and 12/12 gel control sinuses. At 2 weeks postoperatively, the RA-treated sinuses showed an average CBF of 19.78 Hz, which was statistically comparable with the normal unoperated controls (p < 0.26). The inert gel-treated mucosa showed a CBF of 29.24 Hz, which was significantly elevated compared with normals (p < 0.05). At 4 weeks, ciliary activity persisted, but both RA-treated sinuses and gel controls showed elevated CBF compared with normals (p < 0.03). Conclusion Topical RA placed in a demucosalized maxillary sinus yields functional cilia. RA appears to have a normalizing effect on CBF early in the mucosal wound healing process compared with control. This effect appears to be mitigated in later stages of wound healing. RA may be beneficial in enhancing morphological and functional aspects of regenerating cilia.
The Medicago Species A 2-Type Cyclin Is Auxin Regulated and Involved in Meristem Formation But Dispensable for Endoreduplication-Associated Developmental Programs 1

Phytohormones as well as temporal and spatial regulation of the cell cycle play a key role in plant development. Here, we investigated the function and regulation of an alfalfa (Medicago sativa) A2-type cyclin in three distinct root developmental programs: in primary and secondary root development, nodule development, and nematode-elicited gall formation. Using transgenic plants carrying the Medsa;cycA2;2 promoter-glucuronidase gene fusion, in combination with other techniques, cycA2;2 expression was localized in meristems and proliferating cells in the lateral root and nodule primordia. Rapid induction of cycA2;2 by Nod factors demonstrated that this gene is implicated in cell cycle activation of differentiated cells developing to nodule primordia. Surprisingly, cycA2;2 was repressed in the endoreduplicating, division-arrested cells both during nodule development and formation of giant cells in nematode-induced galls, indicating that CycA2;2 was dispensable for S-phase in endoreduplication cycles. Overexpression of cycA2;2 in transgenic plants corresponded to wild type protein levels and had no apparent phenotype. In contrast, antisense expression of cycA2;2 halted regeneration of somatic embryos, suggesting a role for CycA2;2 in the formation or activity of apical meristems. Expression of cycA2;2 was up-regulated by auxins, as expected from the presence of auxin response elements in the promoter. Moreover, auxin also affected the spatial expression pattern of this cyclin by shifting the cycA2;2 expression from the phloem to the xylem poles.
Male dispersal in a provisioned multilevel group of Rhinopithecus roxellana in Shennongjia Nature Reserve, China

Most Old World monkeys show male‐biased dispersal. We present the first systematic data on male dispersal in a provisioned multilevel group of Rhinopithecus roxellana, based on 4.5 years of field observations in Shennongjia National Nature Reserve, China. We evaluated both ultimate (inbreeding avoidance and male mating competition) and proximate (food availability and predation risk) factors influencing male dispersal. The focal group contained 34–53 individuals, in 3–4 one‐male units (OMUs) and 1 all‐male unit (AMU). We observed 37 dispersal events involving 10 of 11 adults, 7 of 8 subadults, and 7 of 15 juveniles. Most interunit transfers within the focal group occurred around the months of mating season. Adult males competed for the leader positions of OMUs mainly through aggressive takeovers, and young males transferred from the OMUs to the AMU at the median age of 41 months, forced out by leader males. No young males older than 4 years remained in natal or non‐natal OMUs. The male mating competition hypothesis was supported. The young males emigrated voluntarily from the focal group at the average age of 58.6 months, and no young emigrating male was observed to return, suggesting inbreeding avoidance also played a role in the dispersal of young males. Most emigration/immigration events were parallel dispersal and occurred during intergroup encounters, suggesting increased predation risk during the dispersal period. Males were more likely to emigrate/immigrate during the months when preferred foods were most available. We compared the dispersal patterns in R. roxellana with those in gelada baboons and hamadryas baboons, both living in multilevel societies. Similar to R. roxellana, young male geladas disperse at puberty, but they may return and breed in their natal groups. Males in hamadryas also disperse, but much less commonly than in R. roxellana. Provisioning may have influenced results, and confirming studies on unprovisioned groups would be valuable. Am. J. Primatol. 73:1280–1288, 2011. © 2011 Wiley Periodicals, Inc.
Screening for antibodies against gliadin in patients with osteoporosis

Abstract. Intestinal disease might contribute to osteopenia. Measurements of IgA antibodies to gliadin have been established as an accepted screening procedure for detection of coeliac disease. When we applied these measurements to 92 patients with verified osteoporosis, 11 subjects (12%) were found to have elevated levels. This is markedly higher than the incidence in healthy subjects (3%). However, the patients with raised levels of IgA antibodies displayed no clinical symptoms and no laboratory evidence of calcium malabsorption. Thus their values for serum calcium, phosphate, parathyroid hormone (PTH), alkaline phosphatase and osteocalcin, as well as the fasting urinary excretion of hydroxyproline and calcium, were similar to those found in other patients with osteoporosis. Intestinal biopsy verified coeliac disease in three patients and was normal in another three. This gives an incidence of verified coeliac disease in this patient group that is approximately tenfold higher than that in the healthy population. Subclinical coeliac disease appears to be unusually over‐represented among patients with idiopathic osteoporosis, and screening for gliadin antibodies might therefore be a valuable addition to the routine assessment of the osteopenic patient. The mechanisms underlying the relationship are not clear, but calcium malabsorption is not evident.
Sustained release of bioactive protein from a lyophilized tissue‐engineered construct promotes the osteogenic potential of mesenchymal stem cells

Tissue‐engineered constructs (TECs) seeded with mesenchymal stem cells (MSCs) represent a therapy for large bone defects. However, massive cell death in TECs in the early postimplantation period prompted us to investigate the osteoinductive mechanism of TECs. Previous studies demonstrated that stem cell extracts retained equivalent levels of bioactive proteins and exhibited an osteoinductive nature similar to that of intact cells. These data led us to hypothesize that despite the massive cell death in TECs, devitalized MSC‐derived proteins remain on the scaffolds and are released to improve cell function. Here, TECs were prepared using demineralized bone matrix seeded with human umbilical cord Wharton's jelly‐derived MSCs (hWJMSCs), and the cells seeded in TECs were devitalized by lyophilizing the TECs. Scanning electron microscopy, BCA protein assays, quantitative cytokine array analysis and immunofluorescent staining indicated that approximately 3 mg/cm3 of total protein and 49 types of cytokines derived from hWJMSCs were preserved in the lyophilized TECs (LTECs). The sustainable release of total protein and cytokines from LTECs lasted for more than 2 weeks. The released protein improved the osteogenic behavior of and gene expression in MSCs. Furthermore, the lyophilized hWJMSC‐derived proteins had immunoregulatory properties similar to those of live MSCs in mixed lymphocyte reactions. Collectively, we present a novel perspective on the osteoinductive mechanism of TECs and introduce LTECs as new systems for delivering multiple cytokines to enhance MSC behavior. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:386–394, 2016.