Miracle-dz/UniFAR-FTUs huggingface dataset:
Hallmarks of basidiomycete soft- and white-rot in wood-decay -omics data of Armillaria

The genus Armillaria spp. (Fungi, Basidiomycota) includes devastating pathogens of temperate forests and saprotrophs that decay wood. Pathogenic and saprotrophic Armillaria species can efficiently colonize and decay woody substrates, however, mechanisms of wood penetration and colonization are poorly known. We assayed the colonization and decay of autoclaved spruce roots using the conifer-specialists Armillaria ostoyae and A. cepistipes using transcriptomic and proteomic data. Transcript and protein levels were altered more extensively in the saprotrophic A. cepistipes than in the pathogenic A. ostoyae and in invasive mycelia of both species compared to their rhizomorphs. Diverse suites of carbohydrate-active enzyme genes (CAZymes), in particular pectinolytic ones and expansins, were upregulated in both species, whereas ligninolytic genes were mostly downregulated. Our gene expression data, together with previous comparative genomic and decay-chemistry analyses suggest that wood decay by Armillaria differs from that of typical white-rot fungi and shows features resembling soft rot. We propose that Armillaria species have modified the ancestral white-rot machinery so that it allows for selective ligninolysis based on environmental conditions and/or host types.
A CHCHD10 variant causing ALS elicits an unfolded protein response through the IRE1/XBP1 pathway

Mutations in CHCHD10, coding for a mitochondrial intermembrane space protein, are a rare cause of autosomal dominant amyotrophic lateral sclerosis (ALS). Mutation-specific toxic gain of function or haploinsuffuciency models have been proposed to explain pathogenicity. To decipher the metabolic dysfunction associated with the haploinsufficient p.R15L variant we integrated transcriptomic, metabolomic and proteomic data sets in patient cells subjected to nutrient stress. Patient cells had a complex I deficiency resulting in an increased NADH/NAD+ ratio, downregulation of the TCA cycle, and a reorganization of one carbon metabolism. This led to phosphorylation of AMPK, activation of an endoplasmic reticulum and mitochondrial unfolded protein response (UPR), and the production of GDF15 and FGF21, which are markers of mitochondrial disease. The endoplasmic reticulum UPR was mediated through the IRE1/XBP1 pathway, and was accompanied by reduced eIF2alpha phosphorylation, dephosphorylation of both JNK isoforms, and up regulation of several dual specific phosphatases. This study demonstrates that loss of CHCHD10 function elicits a striking energy deficit that activates cellular stress pathways, which may underlie the selective vulnerability of motor neurons.
Safety and Efficacy of Ahmed Valve on Intractable Glaucoma in Saudi Population

PURPOSE: The study aims to assess the efficacy and safety of Ahmed glaucoma valve implant in eyes with intractable glaucoma and to evaluate the risk factors for failure. METHODS: A retrospective evaluation of 83 patients (117 eyes) with intractable glaucoma who underwent silicone Ahmed glaucoma valve implant was done in a tertiary care center in Riyadh, Saudi Arabia, between January 2014 and December 2016. Complete success was defined as intraocular pressure (IOP) ≤21 mmHg without medication after a minimum follow-up of 6 months. RESULTS: Eighty-three patients (117 eyes) with intractable glaucoma were evaluated. After a mean follow-up duration of 20.8 ± 3.1 (12–24) months, the overall success rate was achieved in 104 eyes (88.9%). Thirty-six eyes (30.8%) had a complete success rate, whereas 68 eyes (58.1%) had a qualified success rate. Thirteen eyes (11.1%) failed to achieve controlled IOP. The postoperative probability to fail was found to be increasing with time from 0.9% (0.9) in the 1st month to 11.1% (9.9) after 1 year. Complications included a hypertensive phase in 25 eyes (21.4%), encapsulation in 10 eyes (8.5%), hyphema in 7 eyes (6%), hypotony in 2 eyes (1.7%), and blood clots in the implanted tube in 2 eyes (1.7%). The presence of hyphema, scleral patch, and coronary heart diseases was found to increase the risk of failure after Ahmed valve implantation (P = 0.006, 0.040, and 0.014, respectively). CONCLUSIONS: Ahmed glaucoma valve implant was safe and effective in treating cases of intractable glaucoma.
A link between primary and secondary metabolism: malonyl-CoA formation in Streptomyces ambofaciens growing on ammonium ions or valine

Growth of Streptomyces ambofaciens in the presence of valine led to a greater production of spiramycin and excretion of short-chain fatty acids compared with growth on ammonium ions as nitrogen source. We determined the activities of enzymes that lead to the formation of malonyl-CoA, a key intermediate in the biosynthesis of spiramycin. In valine-grown cultures, the activities of acetyl-CoA carboxylase and pyruvate dehydrogenase were increased during the antibiotic production phase. During this idiophase, oxaloacetate dehydrogenase activity was much higher in cultures growing on ammonium compared with valine. Two pathways are proposed concerning the mode of malonyl-CoA formation: a route via oxaloacetate dehydrogenase following growth on ammonium, and a route via acetyl-CoA carboxylase after growth on valine.
A Prospective Study on Safety, Complications and Satisfaction Analysis for Tear Trough Rejuvenation Using Hyaluronic Acid Dermal Fillers

Background: Tear trough (TT) treatment with hyaluronic acid soft tissue fillers is an increasingly popular aesthetic procedure. The traditional needle technique is cited many times in the literature with no studies looking at the results, complications and satisfaction rate with the use of the cannula device instead. The aims of this study are to describe the experience of 4 aesthetic doctors in the treatment of TT deformity and assess complications and side effects, overall satisfaction and improvement. Methods: Twenty-four patients were included (48 TTs) that fulfilled the inclusion and exclusion criteria and they were assessed over a 4-week period, looking at the complications, side effects, satisfaction rate, and others with the cannula technique for the medial TT. Results: Twenty-two women and 2 men each had the medial TT filler supra-periosteally using a cannula device. They were all reviewed at the 2-week stage +/− the 4-week stage. 100% of patients noted an overall improvement to the TTs and 75% were satisfied with their results with the other 25% requiring further filler to be satisfied. There were no major complications and only a small number of minor side effects like mild bruising and swelling that lasted up to 4 weeks. Conclusions: TT treatment, if performed using a cannula with a maximum of 1ml used in one sitting between both eyes, according to this study, is a safe treatment with a very low pain rating and with no major complications and high patient satisfaction.
Morphological and Chemical Alterations of Root Surface after Er:YAG laser, Nd:YAG Laser Irradiation: A Scanning Electron Microscopic and Infrared Spectroscopy Study

Aims and Objectives: This study aimed to evaluate the efficacy of Nd:YAG and Er:YAG lasers in removing the smear layer and to study the morphological and chemical alterations of the root surface using scanning electron microscopy (SEM) and infrared (IR) spectroscopy. Material and Methods: Fifty-five extracted upper incisor teeth were collected and 110 specimens of size 3 mm × 4 mm × 1 mm were prepared. For SEM evaluation, these samples were divided into six groups: A, B, and C. Group A comprised five samples that served as control. Groups B and C were further divided into five subgroups and each subgroup comprised five samples. All the specimens within the subgroups of B and C irradiated with 100, 200, 300, 400, and 500 mJ of Er:YAG laser and 211.66, 423.33, 635, 846.66, and 1058.33 J/cm2 of Nd:YAG laser, respectively. The morphological changes of the laser-treated sites were observed qualitatively using an arbitrary scale under SEM. The data obtained were statistically analyzed by one-way analysis of variance (ANOVA) multiple range test by Turkey’s honestly significant difference and Mann–Whitney U test. In chemical structural changes, Group D comprised five samples that served as nonirradiated control and Groups E and F were irradiated with the same aforementioned parameter and evaluated using Fourier-transform infrared spectroscopy. Results: Er:YAG laser at 100 mJ effectively removed smear layer without any crater formation. The Nd:YAG laser removed the smear layer at the energy density of 211.66 J/cm2 and 423.33J/cm2. The energy density of 1058.33 J/cm2 showed visible charring and deep crater with increased area of melted and resolidified minerals in SEM. In the chemical changes, IR spectroscopy graph showed the reduction in peak intensity beyond 846.66 J/cm2 of and new absorption band was noticed (2010cm–1 and 2017cm–1) at samples treated with 846.66 and 1058.33 J/cm2 of Nd:YAG laser. Conclusion: Er:YAG laser at lower energy density effectively removed smear layer without production of toxic substance as compared with Nd:YAG laser. Thus, Er:YAG laser can be used as an effective root biomodification agent.
Formation of laser-induced periodic surface structures on different materials: fundamentals, properties and applications

Abstract The use of ultra-short pulsed lasers enables the fabrication of laser-induced periodic surface structures (LIPSS) on various materials following a single-step, direct-writing technique. These specific, well-ordered nanostructures with periodicities in the order of the utilised laser wavelength facilitate the engineering of surfaces with functional properties. This review paper discusses the physical background of LIPSS formation on substrates with different material properties. Using the examples of structural colours, specific wetting states and the reduction of friction and wear, this work presents experimental approaches that allow to deliberately influence the LIPSS formation process and thus tailor the surface properties. Finally, the review concludes with some future developments and perspectives related to forthcoming applications of LIPSS-based surfaces are discussed.
The influence of the family on the formation of eating and weight disorders

Abstract The аim is to study family influence on formation of eating and weight disorders. The concept of an “alimentary family” is defined as a family with dysfunctional, disharmonious relationships, which is a prerequisite for emergence and support of distorted patterns of eating behaviour, leading in the future to children’s eating and weight disorders. Methods: The research was carried out using the method of a thematic retrospective analysis (MTRA)-food, which is a variant of the narrative method, the questionnaire “Parental convictions and control tactics as for eating behaviour of their children during food taking”. The data was processed by the content analysis method; Fisher’s φ-criterion was used to compare differences between the groups. Results: The research has allowed us to clarify eating behavioural characteristics and to identify the “roots” of eating disorders. Various forms of forcing at eating, direct and indirect ways of making children to eat or blocking of eating are manifested in ignoring of children’s taste preferences, their desire and readiness to eat. Parents often use manipulative techniques influencing children’s eating behaviour (encouragement, inducement, reward promises, approval, recognition, warning, or switching attention), direct means of influence (coercion: prohibition, restriction, rejection, destructive criticism, intimidation, deprivation from various pleasures). There is the statistical confirmation that parents’ use of manipulative means and / or direct coercion towards their children during eating predetermines formation of pathological processes of corporeality, attitudes and psychological mechanisms stipulating eating disorders. Conclusions: The research results indicate necessity to develop psychotherapeutic programs for people with eating disorders, as well as programs to help parents improve family relationships and, accordingly, to apply correctional effects on their children.
Overcoming the Effects of Restrictive Fiscal Policy: The Case of Argentina and Serbia

Abstract The paper analyses one of the most important economic issues relevant to most countries. The issue involves how to as painless as possible overcome the problems of high budget deficits and excessive accumulated public debt. Argentina and Serbia are used as an example. Argentina implemented rigorous saving measures in 2002 and Serbia began to implement restrictive budgetary measures in 2014. The effects of such a policy can be designed for the future. Results indicate that the key to Argentina’s success lies in the transition to a floating exchange rate and the high level of correlation between the growth of the foreign exchange rate and growth in exports. When comparing strict fiscal policy in Argentina and Serbia, it should be emphasised that the measures in Serbia are far less stringent than those that were established in Argentina. But it also means that the effect of reducing budget expenditures should have less of an impact on GDP reduction than in the case of Argentina.
The relationship between species richness and biomass changes from boreal to subtropical forests in China

Diversity-manipulation experiments suggest a positive effect of biodiversity on ecosystem properties (EPs), but variable relationships between species richness and EPs have been reported in natural communities. An explanation for this discrepancy is that observed richness–EPs relationships in natural communities change with environment and species functional identities. But how the relationships change along broad-scale climatic gradients has rarely been examined. In this paper, we sampled 848 plots of 20 × 30 m2 from boreal to tropical forests across China. We examined plot biomass with respect to environmental factors, tree species richness and functional group identity (FGI, i.e. evergreen vs deciduous, and coniferous vs broadleaf). Variation partitioning was used to evaluate the relative effects of the three classes of factors. We found that, most of the ‘effects’ (percentage of variation explained) of richness and FGI on forest biomass were shared with environmental factors, but species richness and FGI still revealed significant effects in addition to environment for plots across China. Richness and FGI explained biomass mainly through their shared effects instead of independent effects, suggesting that the positive biodiversity effect is closely associated with a sampling effect. The relative effects of richness, FGI and environment varied latitudinally: the independent effects of environment and richness decreased from boreal to subtropical forests, whereas the total effect of FGI increased. We also found that the slope of richness–biomass relationship decreased monotonically from boreal to subtropical forests, possibly because of decreasing complementarity and increasing competition with increasing productivity. Our results suggest that while species richness does have significant effects on forest biomass it is less important than environmental gradients and other biotic factors in shaping large-scale biomass patterns. We suggest that understanding how and why the diversity–EPs relationships change along climatic gradient would be helpful for a better understanding of real biodiversity effects in natural communities.
Physiological Response to Non-Traditional High-Intensity Interval Training

Summary High intensity interval training (HIIT) is an exercise program not only for professional athletes, but also for the general population. Usually, one-dimensional modalities such as running or a cycling simulator are used. There also exist protocols that use the HIIT principles but apply full-body exercises (HWT). The purpose of the study was to verify the response to unconventional loads based on HIIT and HWT protocols using the near infrared spectroscopy (NIRS) and spiroergometry: wall ball (WB); SKI ergometer, toes to bar (TTB) and assault air bike (AB) in a selected proband. Working interval was 60 s resp. 30 s, the rest between sites was progressively reduced from 60 s to 30 s. The proband completed a total of 3 laps. The results showed that the load applied had been similar to that of HWT or HIIT, where the effect on cardiorespiratory and metabolic functions was confirmed. Acute changes in the observed parameters of SmO2 in m. vastus lateralis indicate a possible effect on the development of strength capabilities. It has also been confirmed that the application of variable types of load can be applied simultaneously with the adjustment of rest time and thus using conditions that can reflect current options (material, environment, time) and specific goals. The measured values of muscle tissue oxidation, carbon dioxide output, heart rate confirm that a similar type of load can be a suitable means of affecting cardiovascular and metabolic functions.
Environmental Changes Affecting the Migratory Timing of American Shad and Sockeye Salmon

The rate of evolution and the scope for phenotypic plasticity can be assessed by studying the adaptation of an introduced population to a new habitat and the response of established populations to progressive environmental change. Adult American shad (Alo- sa sapidissima), an introduced species, and sockeye salmon (Oncorhynchus nerka), a native species, migrate up the Columbia River (northwestern United States) in late spring and early summer to spawn. Based on records from Bonneville Dam, the river's spring warming has occurred progressively earlier since :1950, coinciding with a reduction in spring discharge. The date when 50% of the shad migrated past the dam is correlated with this shift in thermal and flow regimes; they now ascend the river -38 d earlier than they did in 1938. However, the mean temperature that they experience has actually decreased by 1.80 C in 45 yr, indicating that the change in their migratory timing has outstripped the rate of environmental change. The upriver migration of sockeye salmon is also earlier than in past years, but their change in timing (-6 d since 1949) lags behind the rate of envi- ronmental change, and they are now experiencing ~2.50C warmer temperatures than in past years. We hypothesize that the differences in response to changing environmental conditions between these species arise from differences in their migration patterns and early life histories. Shad spawn soon after they enter the river in its main stem where environ- mental conditions of the larvae will closely mirror those experienced by upstream-migrating adults. They may therefore have evolved a migratory pattern that allows greater behavioral response to environmental fluctuations than sockeye salmon, which spawn in distant lo- cations many months after their upriver migration. Sockeye salmon migration may be more strongly controlled by innate responses to photoperiod, migrating at the time of year which is best on average because conditions in the lower river will not be indicative of those to be experienced by incubating embryos and juveniles.
Explosive Strength of Lower Limbs in 10 – 12 Years Old Athletes of Individual Sports

Summary The aim of the study was to determine the levels of lower limb explosive strength of girls in different sport specialization. The sample consisted of 24 girls in aerobic gymnastics (n = 12) and athletic (n = 12) aged 10 to 12 years. To assess the levels of girls’ lower limbs explosive strength, we administered the following tests: countermovement jump test, countermovement jump with free arms test, squat jump test, 10-seconds repetitive jumping test. Testing sessions took place in September 2019. To obtain data about the levels of lower limbs explosive strength, we recorded step height (cm) and duration of the flight phase (s). Data about the lower limbs explosive strength were collected using the Optogait system for optical detection. We applied basic statistical characteristics, namely Student’s t-test and multivariate linear regression. There were no significant differences between girls in aerobic gymnastics and athletics. We recorded better test results in the group of girl’s athletes in the countermovement jump, countermovement jump with free arms and squat jump. In the group of girl’s involved in aerobic gymnastics, we recorded better results in the 10-second repetitive vertical jumping and vertical jump strength. According to the collected data, we may conclude that the training process of the selected girls in terms of explosive strength development is significantly different. Girls involved in aerobic gymnastics showed a higher level of performance in the 10-second vertical jump, which results from the structure of sport specificity.
Disassembling Papaver: a restriction site analysis of chloroplast DNA

The results from a chloroplast DNA restriction site analysis of the genera Papaver and Roemeria of subf. Papaveroideae (Papaveraceae) and five outgroup taxa are presented. Papaver is represented by 14 species of eight of the 11 sections recognized, Roemeria by two of its three species. Hunnemannia fumariifolia (subf. Eschscholzioideae), Chelidonium majus (subf. Chelidonioideae), Romneya coulteri, Argemone munita and Stylomecon heterophyllum (all subf. Papaveroideae) were chosen as outgroups. DNAs were digested with 24 restriction enzymes. The major results from this analysis are: 1. Papaver, Roemeria and Stylomecon form a monophyletic group supported by at least 17 restriction site mutations and a 150 to 300 base pair deletion in the inverted repeat of the chloroplast genome. 2. Both Stylomecon and Roemeria are nested within Papaver in its traditional circumscription. 3. Of these, Roemeria is nested within P. sect. Argemonidium and is the sister group to P. pavoninum. 4. Dependent on the mode of character weighting chosen, P. sect. Meconella either is the sister group to P. sect. Argemonidium + Roemeria or of P. sect. Argemonidium + Roemeria + Stylomecon+ the remainder of Papaver (Papaver s. str.). 5. Stylomecon is the sister group to Papaver s. str. (excluding sects Meconella and Argemonidium). These results are discussed on the background of other available systematic evidence.
On the Chalcidoid Parasites of Psyllids (Hemiptera, Homoptera).

While engaged in working out some small lots of Psyllid parasites from various parts of the world, I have found it necessary to examine, as far as possible, the records of Chalcids which up to the present have been bred from these hosts. The literature has proved to be more extensive than had been anticipated, and it has therefore seemed well to bring together and list these scattered references when describing the forms that have been considered as new.