Miracle-dz/UniFAR-FTUs huggingface dataset:
Impaired GABAergic and glutamatergic neurometabolic activity in aged mice brain as measured by 1H‐[13C]‐NMR spectroscopy

Healthy aging is associated with a decline in cognitive function, and is a major risk factor for many neurodegenerative diseases. Although, there are several evidence that brain mitochondrial function is altered with aging its significance at the cellular level is elusive. In this study, we have investigated mitochondrial TCA cycle and neurotransmitter cycle fluxes associated with glutamatergic, GABAergic neurons and astroglia in the cerebral cortex and hippocampus of young (6 months) and aged (24 months) C57BL6 mice by using 1H‐[13C]‐NMR spectroscopy together with timed infusion of 13C‐labeled glucose and acetate. The ratio VCyc/VTCA was determined from a steady‐state [2‐13C]acetate experiment. Metabolic fluxes were obtained by fitting a three‐compartment metabolic model to 13C turnover of amino acids from glucose. Levels of glutamate, aspartate and taurine were reduced in the cerebral cortex, while glutamine and choline were elevated in the hippocampus of aged mice. Interestingly, the rate of acetate oxidation increased in the cerebral cortex, while the flux of mitochondrial TCA cycle of glutamatergic neurons decreased in the cerebral cortex (P < .0001) and hippocampus (P = .025) of aged mice. The glutamate‐glutamine neurotransmitter cycle flux was reduced in the cerebral cortex (P < .0001). The GABAergic TCA cycle flux was reduced in the cerebral cortex (P = .0008), while GABA‐glutamine neurotransmitter cycling flux was also reduced in the cerebral cortex (P = .011) and hippocampus (P = .042) of aged brain. In conclusion, the reduction in excitatory and inhibitory neurotransmitter activity of glutamatergic and GABAergic neurons in the cerebral cortex and hippocampus correlates qualitatively with declined cognitive function in aged mice.
Engineering and Assessing Cardiac Tissue Complexity

Cardiac tissue engineering is very much in a current focus of regenerative medicine research as it represents a promising strategy for cardiac disease modelling, cardiotoxicity testing and cardiovascular repair. Advances in this field over the last two decades have enabled the generation of human engineered cardiac tissue constructs with progressively increased functional capabilities. However, reproducing tissue-like properties is still a pending issue, as constructs generated to date remain immature relative to native adult heart. Moreover, there is a high degree of heterogeneity in the methodologies used to assess the functionality and cardiac maturation state of engineered cardiac tissue constructs, which further complicates the comparison of constructs generated in different ways. Here, we present an overview of the general approaches developed to generate functional cardiac tissues, discussing the different cell sources, biomaterials, and types of engineering strategies utilized to date. Moreover, we discuss the main functional assays used to evaluate the cardiac maturation state of the constructs, both at the cellular and the tissue levels. We trust that researchers interested in developing engineered cardiac tissue constructs will find the information reviewed here useful. Furthermore, we believe that providing a unified framework for comparison will further the development of human engineered cardiac tissue constructs displaying the specific properties best suited for each particular application.
Growth failure: ‘idiopathic’ only after a detailed diagnostic evaluation

The terms ‘idiopathic short stature’ (ISS) and ‘small for gestational age’ (SGA) were first used in the 1970s and 1980s. ISS described non-syndromic short children with undefined aetiology who did not have growth hormone (GH) deficiency, chromosomal defects, chronic illness, dysmorphic features or low birth weight. Despite originating in the pre-molecular era, ISS is still used as a diagnostic label today. The term ‘SGA’ was adopted by paediatric endocrinologists to describe children born with low birth weight and/or length, some of whom may experience lack of catch-up growth and present with short stature. GH treatment was approved by the FDA for short children born SGA in 2001, and by the EMA in 2003, and for the treatment of ISS in the US, but not Europe, in 2003. These approvals strengthened the terms ‘SGA’ and ‘ISS’ as clinical entities. While clinical and hormonal diagnostic techniques remain important, it is the emergence of genetic investigations that have led to numerous molecular discoveries in both ISS and SGA subjects. The primary message of this article is that the labels ISS and SGA are not definitive diagnoses. We propose that the three disciplines of clinical evaluation, hormonal investigation and genetic sequencing should have equal status in the hierarchy of short stature assessments and should complement each other to identify the true pathogenesis in poorly growing patients.
Condensed tannins in the diet of folivorous diademed sifakas and the gap between crude and available protein

Tannins, a type of plant secondary metabolite, are well‐known for their ability to precipitate proteins and thereby reduce the protein available to consumers. Most primate studies have focused on condensed tannins (CTs) as they were thought to be the most effective type of tannin at preventing protein acquisition, but there is growing recognition that other types of tannins can bind to proteins, suggesting the division among tannin types is not as clear‐cut as previously thought. Although previous studies have documented the presence of CTs in primate diets and primates' behavioral responses to them, our understanding of tannins remains limited because few researchers have used Sephadex column purification to accurately determine tannin concentrations, and few have used in vitro assays to determine available protein content and the tannins' effectiveness in binding protein. In this study, we documented diademed sifaka (Propithecus diadema) diet from June to August 2018 at Tsinjoarivo, Madagascar (in two forests with varying degrees of habitat disturbance) and quantified CT concentration and actual available protein in foods. Eleven of the fourteen top foods tested contained CTs (concentrations: 4.8%–39.3% dry matter). An in vitro assay showed available protein was strikingly low in six of the eleven top foods (e.g., little to no apparent available protein, despite high crude protein). Overall, our findings suggest sifakas acquire less protein than previously recognized and probably have adaptations to counteract tannins. Such studies of available protein are critical in understanding dietary constraints on sifaka populations and the evolution of their diet choice strategies; despite the conventional wisdom that leaves are protein‐rich, folivorous primates may indeed be protein‐limited. However, further studies are necessary to determine if sifakas have counter‐adaptations to tannins, and if they absorb more protein than our analyses suggest, perhaps receiving protein that we were unable to detect with the current techniques (e.g., pollen).
Analysis of Physicochemical Parameters of Congress Worts Prepared from Special Legume Seed Malts, Acquired with and without Use of Enzyme Preparations

This study was conducted to produce malt from legume seeds (chickpea, lentil, pea, and vetch) and test whether malting with parameters, typically barley grain, will result in well-modified legume seed malt. Analysis of malt was performed by producing congress worts from legume seed malts. Concentration of phenolic compounds, as well as antioxidant activity of legume seed malts was analysed. Acquired worts were characterised with poor technological characteristics (wort extract, wort volume, saccharification time, brewhouse efficiency); however, the malting process increased concentration of phenolic compounds and antioxidant activity of the plant material. Subsequent mashing tests with addition of different external enzymes and/or gelatinisation of legume seed malt were performed. Use of external enzymes improved saccharification time, extract content, wort volume, as well brewhouse efficiency in the case of some legume seed malts. The best brewhouse efficiencies and highest extract values were acquired by the samples prepared with 30% of gelatinised vetch malt or chickpea malt mixed with 70% of Pilsner malt. The study shows that there is possibility of creating legume seed malts, but malting and mashing characteristics need to be customised for these special malts.
Modulation of Human Sperm Mitochondrial Respiration Efficiency by Plant Polyphenols

Plant bioactives, such as polyphenols, can differentially affect (positively or negatively) sperm quality, depending on their concentration. These molecules have been proposed as natural scavengers of reactive oxygen species (ROS) for male infertility treatment. However, few data are available about their effects on the molecular mechanisms related to sperm quality and, in particular, to sperm mitochondrial function. We investigated the effects of quercetin, naringenin, genistein, apigenin, luteolin, and resveratrol at the concentration of 0.1–1000 nM on mitochondrial respiration efficiency. Upon chemical exposure, spermatozoa were swollen in a hypotonic solution and used for polarographic assays of mitochondrial respiration. All tested compounds, except for apigenin, caused a significant increase in the mitochondrial respiration efficiency at the concentration of 0.1 nM, and a significant decrease starting from concentrations of 10 nM. The analysis of oxygen consumption rate in the active and in the resting state of mitochondrial respiration suggested different mechanisms by which the tested compounds modulate mitochondrial function. Therefore, by virtue of their ability to stimulate the respiration active state, quercetin, genistein, and luteolin were found to improve mitochondrial function in asthenozoospermic samples. Our results are relevant to the debate on the promises and perils of natural antioxidants in nutraceutical supplementation.
Foam stability: Part III. Spontaneous foam destabilization resulting from gas diffusion

Bubble size frequency distributions in foamed Tee-pol solutions, oil-in-water emulsions and Hevea rubber latex have been calculated from the observed bubble sizes in the plane of contact between the foam and the glass wall of the container. The bubble size distribution in these foams can be satisfactorily described by the same mathematical expression containing one adjustable parameter. Using this expression for the distribution function, a relation is derived between the total number of bubbles per unit area of the cross-section and the age of the foam, assuming that the disappearance of the small bubbles is caused only by transfer of gas to larger bubbles. With the aid of the derived relationship the average thickness of the liquid lamellae in a number of foams is calculated from the observed rate of decrease in the number of bubbles in the front layer. The calculated values of the average film thickness are of the same order of magnitude as those calculated directly from the observed bubble size distribution and the liquid content of the foam. Additional evidence of the proposed destabilization mechanism is obtained from experiments, in which the nature of the gaseous phase was varied and from comparison of the rate of decrease in the number of bubbles with the rate of shrinking of individual bubbles.
Temporal and Spatial Blood Feeding Patterns of Urban Mosquitoes in the San Juan Metropolitan Area, Puerto Rico

Simple Summary Understanding the biodiversity of urban ecosystems is critical for management of invasive and pest species, conserving native species, and disease control. Mosquitoes (Culicidae) are ubiquitous and abundant in urban ecosystems, and rely on blood meals taken from vertebrates. We used DNA from freshly blood-fed mosquitoes to characterize the diversity of vertebrate host species in the San Juan Metropolitan Area, Puerto Rico. We collected two mosquito species that fed on a variety of vertebrates. Culex quinquefasciatus fed on 17 avian taxa (81.2% of blood meals), seven mammalian taxa (17.9%), and one reptilian taxon (0.85%). Aedes aegypti blood meals were from a less diverse group, with two avian taxa (11.1%) and three mammalian taxa (88.9%) identified. Domestic chickens dominated the blood meals of Cx. quinquefasciatus, both temporally and spatially, and no statistically significant shift from birds to mammals was detected. The species we detected from the mosquito blood meals provided a snapshot of the vertebrate community in the San Juan Metropolitan Area, most of which were domestic species. However, we also identified a variety of native and nonnative wild species. These results add knowledge about potential ecological factors that impact vector-borne disease management in urban habitats. Abstract Urban ecosystems are a patchwork of habitats that host a broad diversity of animal species. Insects comprise a large portion of urban biodiversity which includes many pest species, including those that transmit pathogens. Mosquitoes (Diptera: Culicidae) inhabit urban environments and rely on sympatric vertebrate species to complete their life cycles, and in this process transmit pathogens to animals and humans. Given that mosquitoes feed upon vertebrates, they can also act as efficient samplers that facilitate detection of vertebrate species that utilize urban ecosystems. In this study, we analyzed DNA extracted from mosquito blood meals collected temporally in multiple neighborhoods of the San Juan Metropolitan Area, Puerto Rico to evaluate the presence of vertebrate fauna. DNA was collected from 604 individual mosquitoes that represented two common urban species, Culex quinquefasciatus (n = 586) and Aedes aegypti (n = 18). Culex quinquefasciatus fed on 17 avian taxa (81.2% of blood meals), seven mammalian taxa (17.9%), and one reptilian taxon (0.85%). Domestic chickens dominated these blood meals both temporally and spatially, and no statistically significant shift from birds to mammals was detected. Aedes aegypti blood meals were from a less diverse group, with two avian taxa (11.1%) and three mammalian taxa (88.9%) identified. The blood meals we identified provided a snapshot of the vertebrate community in the San Juan Metropolitan Area and have potential implications for vector-borne pathogen transmission.
Stability of Proton Exchange Membranes in Phosphate Buffer for Enzymatic Fuel Cell Application: Hydration, Conductivity and Mechanical Properties

Proton-conducting ionomers are widespread materials for application in electrochemical energy storage devices. However, their properties depend strongly on operating conditions. In bio-fuel cells with a separator membrane, the swelling behavior as well as the conductivity need to be optimized with regard to the use of buffer solutions for the stability of the enzyme catalyst. This work presents a study of the hydrolytic stability, conductivity and mechanical behavior of different proton exchange membranes based on sulfonated poly(ether ether ketone) (SPEEK) and sulfonated poly(phenyl sulfone) (SPPSU) ionomers in phosphate buffer solution. The results show that the membrane stability can be adapted by changing the casting solvent (DMSO, water or ethanol) and procedures, including a crosslinking heat treatment, or by blending the two ionomers. A comparison with NafionTM shows the different behavior of this ionomer versus SPEEK membranes.
Fracture and Contact Adhesion Energies of Mica‐Mica, Silica‐Silica, and Mica‐Silica Interfaces in Dry and Moist Atmospheres

A study is made of the factors that contribute to the energy of mica-mica, silica-silica, and mica-silica interfaces in the presence of moist atmospheres. Energies are measured using brittle fracture and contact adhesion techniques. Both “virgin” and “healed” interfaces are investigated, with special attention on the latter. The fracture and adhesion data overlap, reflecting a common underlying separation process by “sharp-crack propagation. ” The study identifies several contributors to the interface adhesion energies. At virgin mica-mica and silica-silica interfaces the energy is determined by primary ionic-covalent attraction, and by the screening of this attraction by condensed moisture from the atmosphere. At healed interfaces the energy depends on both environmental interaction and “lattice” coherence. At retracted cracks in mica-mica most of the virgin ionic attraction is retained. On misorienting separated cleavage halves prior to recontact the interaction energy drops substantially: in “dry” atmosphers (relative humidity 50%) capillary forces dominate. The dissimilar mica-silica system exhibits the same dominance by capillary forces in wet atmospheres. However, in dry atmospheres the adhesion energy becomes inordinately high, from spontaneous transfer of electronic charge from one surface to the other. The implications of these observations concerning mechanical properties of brittle ceramics are discussed.
Is the Occurrence of the Sticking Region in Maximum Smith Machine Squats the Result of Diminishing Potentiation and Co-Contraction of the Prime Movers among Recreationally Resistance Trained Males?

This study compared the kinetics, barbell, and joint kinematics and muscle activation patterns between a one-repetition maximum (1-RM) Smith machine squat and isometric squats performed at 10 different heights from the lowest barbell height. The aim was to investigate if force output is lowest in the sticking region, indicating that this is a poor biomechanical region. Twelve resistance trained males (age: 22 ± 5 years, mass: 83.5 ± 39 kg, height: 1.81 ± 0.20 m) were tested. A repeated two-way analysis of variance showed that Force output decreased in the sticking region for the 1-RM trial, while for the isometric trials, force output was lowest between 0–15 cm from the lowest barbell height, data that support the sticking region is a poor biomechanical region. Almost all muscles showed higher activity at 1-RM compared with isometric attempts (p < 0.05). The quadriceps activity decreased, and the gluteus maximus and shank muscle activity increased with increasing height (p ≤ 0.024). Moreover, the vastus muscles decreased only for the 1-RM trial while remaining stable at the same positions in the isometric trials (p = 0.04), indicating that potentiation occurs. Our findings suggest that a co-contraction between the hip and knee extensors, together with potentiation from the vastus muscles during ascent, creates a poor biomechanical region for force output, and thereby the sticking region among recreationally resistance trained males during 1-RM Smith machine squats.
Immunity in Space: Prokaryote Adaptations and Immune Response in Microgravity

Immune dysfunction has long been reported by medical professionals regarding astronauts suffering from opportunistic infections both during their time in space and a short period afterwards once back on Earth. Various species of prokaryotes onboard these space missions or cultured in a microgravity analogue exhibit increased virulence, enhanced formation of biofilms, and in some cases develop specific resistance for specific antibiotics. This poses a substantial health hazard to the astronauts confined in constant proximity to any present bacterial pathogens on long space missions with a finite number of resources including antibiotics. Furthermore, some bacteria cultured in microgravity develop phenotypes not seen in Earth gravity conditions, providing novel insights into bacterial evolution and avenues for research. Immune dysfunction caused by exposure to microgravity may increase the chance of bacterial infection. Immune cell stimulation, toll-like receptors and pathogen-associated molecular patterns can all be altered in microgravity and affect immunological crosstalk and response. Production of interleukins and other cytokines can also be altered leading to immune dysfunction when responding to bacterial infection. Stem cell differentiation and immune cell activation and proliferation can also be impaired and altered by the microgravity environment once more adding to immune dysfunction in microgravity. This review elaborates on and contextualises these findings relating to how bacteria can adapt to microgravity and how the immune system subsequently responds to infection.
Wearable 3D Machine Knitting: Automatic Generation of Shaped Knit Sheets to Cover Real-World Objects

Knitting can efficiently fabricate stretchable and durable soft surfaces. These surfaces are often designed to be worn on solid objects as covers, garments, and accessories. Given a 3D model, we consider a knit for it wearable if the knit not only reproduces the shape of the 3D model but also can be put on and taken off from the model without deforming the model. This “wearability” places additional constraints on surface design and fabrication, which existing machine knitting approaches do not take into account. We introduce the first practical automatic pipeline to generate knit designs that are both wearable and machine knittable. Our pipeline handles knittability and wearability with two separate modules that run in parallel. Specifically, given a 3D object and its corresponding 3D garment surface, our approach first converts the garment surface into a topological disc by introducing a set of cuts. The resulting cut surface is then fed into a physically-based unclothing simulation module to ensure the garment’s wearability over the object. The unclothing simulation determines which of the previously introduced cuts could be sewn permanently without impacting wearability. Concurrently, the cut surface is converted into an anisotropic stitch mesh. Then, our novel, stochastic, any-time flat-knitting scheduler generates fabrication instructions for an industrial knitting machine. Finally, we fabricate the garment and manually assemble it into one complete covering worn by the target object. We demonstrate our method’s robustness and knitting efficiency by fabricating models with various topological and geometric complexities. Further, we show that our method can be incorporated into a knitting design tool for creating knitted garments with customized patterns.