Miracle-dz/UniFAR-FTUs huggingface dataset:
MiR-613 suppresses retinoblastoma cell proliferation, invasion, and tumor formation by targeting E2F5

Retinoblastoma is a common intraocular malignancy that occurs during childhood. MicroRNAs play critical roles in the regulation of retinoblastoma initiation and progression, and aberrant expression of miR-613 had been reported in various types of cancer. However, the role and mechanism of its function in retinoblastoma are still unclear. In this study, we found that miR-613 was downregulated in retinoblastoma tissues and cell lines. Overexpression of miR-613 suppressed retinoblastoma cell proliferation, migration, and invasion and induced cell cycle arrest in vitro. Additionally, overexpressed miR-613 also inhibited tumor formation of retinoblastoma cells in vivo. We further identified E2F5 as a direct target of miR-613. Reintroduction of E2F5 without 3′-untranslated region reversed the inhibitory effects of miR-613 on cell proliferation and invasion. Our data collectively indicate that miR-613 functions as a tumor suppressor in retinoblastoma through downregulating E2F5, supporting the targeting of the novel miR-613/E2F5 axis as a potentially effective therapeutic approach for retinoblastoma.
Processing optimization and characterization of gelatin from catfish (Clarias gariepinus) skin

The extraction of catfish (Clarias gariepinus) skin gelatin was optimized by using response surface methodology (RSM) employing a central composite design (CCD). RSM with 3-factors, 5-levels CCD was carried out for the optimization. The independent variables were suggested which include NaOH concentration (0.07-0.23 mol/L), acetic acid concentration (0.04-0.14 mol/L) and extraction temperature (40-80°C) with the percentage of hydroxyproline recovery (Y) as dependant variable. A maximum Y of 65.32% for gelatin processing was obtained using a combination of 0.13 mol/L NaOH and 0.09 mol/L acetic acid for 1 h, followed by a hot-water extraction at 64.92°C for 3 h. The results indicated a high protein content (88.46 g/100 g) in the extracted gelatin with a viscosity of 3.45 mPa.s, 286.71 g gel strength and 173 residues per 1000 residues of imino acids (proline and hydroxyproline). Furthermore, the gelatin from catfish also showed a relatively good instrumental texture quality according to texture profile analysis (TPA).
[Clinical observation on governor vessel Daoqi method for treatment of dyssomnia in the patient of depression].

OBJECTIVE To explore clinical therapeutic effects of Governor Vessel Daoqi needling method combined with antidepressants on dyssomnia in the patient of depression. METHODS depression with dyssomnia were randomly divided into a treatment group (n=23) and a control group (n=22). The treatment group were treated with Governor Vessel Daoqi needling method and oral administration of antidepressants, with Shenting (GV 24), Baihui (GV 20), Dazhui (GV 14), Shendao (GV 11) and Zhiyang (GV 9) selected as main acupoints; and the control group with simple antidepressants. They were treated for 4 weeks. Changes of scores for Hamilton Depression Rating Scale (HAMD) and Pittsburgh Sleep Quality Index (PSQI) were investigated in the two groups. RESULTS Significant differences before and after treatment in the scores for HAMD (P < 0.01) in both the two groups, and a significant difference before and after treatment in the score for PSQI only in the treatment group (P < 0.01) were found; after treatment, there were significant differences between the two groups in the scores of HAMD and PSQI (P < 0.01). CONCLUSION The combined therapy of Governor Vessel Daoqi needling method and antidepressants can significantly improve dyssomnia in the patient of depression.
Sharing hardware resources in heterogeneous computer-supported collaboration scenarios

There currently are many mobile computing devices with various properties and capabilities. These devices may need to collaborate among them to allow nomad workers to perform a common activity. Unfortunately software developers in charge of creating infrastructures or applications allowing these devices to cooperate among them, do not count with clear guidelines to design such software components; particularly when these components must work in a scenario involving heterogeneous devices. This paper presents a study that tries to understand how to address collaboration among heterogeneous mobile devices, by exploring several variables affecting the process. In particular, this study explores various strategies to borrow CPU slots from peer mobile computing devices and return the favor back later on. The study outcomes indicate there is a short list of computing and network variables affecting the collaboration capability of the mobile devices. These findings have been verified using data mining techniques. Based on these findings and the lessons learned, the article presents a simulation method of computing scenarios that can help developers to determine which computing configuration would be suitable to be used in each particular work scenario. Software designers can take advantage of this simulation method and the design guidelines reported in this paper in order to develop applications able to work appropriately in heterogeneous computing scenarios.
Analysis of individualized education programs to quantify long-term educational needs following surgical intervention for single-suture craniosynostosis.

BACKGROUND Single-suture craniosynostosis (SSC) is a common craniofacial condition with potential neurocognitive sequelae. OBJECTIVE To quantify any long-term functional academic and behavioural difficulties of children with SSC as indicated by the need for individualized education programs (IEPs), despite having undergone surgical treatment. METHODS Records of all school-age patients from 1992 to 2011 who underwent operative intervention for SSC were identified. Fifty-nine patients' guardians were contacted by telephone to provide informed consent for completion of a mailed standardized questionnaire querying demographic information as well as information regarding the patient's health, family and educational history; specifically whether the patient had ever been provided educational support as delineated in an IEP. The primary outcome measure was the history of the patient being assigned educational support as delineated in an IEP. RESULTS Thirty-seven consenting guardians completed and returned the standardized questionnaire (response rate 62.7%). Twenty-one patients were male and 16 were female, with an age range of five to 14 years (mean age 10.2 years). Eleven (29.7%) patients had a previous history of or currently were receiving educational support delineated in an IEP. CONCLUSIONS A higher proportion of school-age patients with a history of SSC (status postsurgical intervention) in the present study received educational support delineated in an IEP than the proportion of IEPs in the general student population of the United States (11.3%).
The Kaposi's Sarcoma-Associated Herpesvirus K5 E3 Ubiquitin Ligase Modulates Targets by Multiple Molecular Mechanisms

ABSTRACT Kaposi's sarcoma-associated herpesvirus encodes two highly related membrane-associated, RING-CH-containing (MARCH) family E3 ubiquitin ligases, K3 and K5, that can down regulate a variety of cell surface proteins through enhancement of their endocytosis and degradation. In this report we present data that while K5 modulation of major histocompatibility complex class I (MHC-I) closely mirrors the mechanisms used by K3, alternative molecular pathways are utilized by this E3 ligase in the down regulation of intercellular adhesion molecule 1 (ICAM-1) and B7.2. Internalization assays demonstrate that down regulation of each target can occur through increased endocytosis from the cell surface. However, mutation of a conserved tyrosine-based endocytosis motif in K5 resulted in a protein lacking the ability to direct an increased rate of MHC-I or ICAM-1 internalization but still able to down regulate B7.2 in a ubiquitin-dependent but endocytosis-independent manner. Further, mutation of two acidic clusters abolished K5-mediated MHC-I degradation while only slightly decreasing ICAM-1 or B7.2 protein destruction. This same mutant abolished detectable ubiquitylation of all targets. These data indicate that while K5 can act as an E3 ubiquitin ligase to directly mediate cell surface molecule destruction, regulation of its targets occurs through multiple pathways, including ubiquitin-independent mechanisms.
Generalized Coupling Coefficient for Filters With Nonresonant Nodes

In this letter, we introduce a generalization of the coupling coefficient definition that applies to filters, including nonresonating nodes. The novel coefficients are evaluated from the synthesized low-pass prototype (suitable formulas are given); they allow an easier prototype de-normalization because no complicated network transformation is requested. Several different solutions with preassigned network parameters can be easily obtained by exploiting the degree of freedom offered by the de-normalized network. Some examples illustrating the use of the new coefficient are given.
Statistical reliability assessment of software-based systems

Plant vendors nowadays propose software-based systems even for the most critical safety functions. The reliability estimation of safety critical software-based systems is difficult since the conventional modeling techniques do not necessarily apply to the analysis of these systems, and the quantification seems to be impossible. Due to lack of operational experience and due to the nature of software faults, the conventional reliability estimation methods can not be applied. New methods are therefore needed for the safety assessment of software-based systems. In the research project "Programmable automation systems in nuclear power plants (OHA)", financed together by the Finnish Centre for Radiation and Nuclear Safety, the Ministry of Trade and Industry and the Technical Research Centre of Finland, various safety assessment methods and tools for software based systems are developed and evaluated. This volume in the OHA-report series deals with the statistical reliability assessment of software based systems on the basis of dynamic test results and qualitative evidence from the system design process. Other reports to be published later on in OHA-report series will handle the diversity requirements in safety critical software-based systems, generation of test data from operational profiles and handling of programmable automation in plant PSA-studies.
A population of caudally migrating cranial neural crest cells: functional and evolutionary implications.

The deployment of the cranial neural crest is central to the patterning of the skeletomuscular elements of the vertebrate head, with cranial muscles invariably attaching to skeletal elements formed by crest from the same axial level. Here we demonstrate, through gene expression analysis, ablation studies and fate-mapping, the existence of a population of caudally migrating cranial crest that arise from the postotic neural tube. As with the rest of the postotic crest, these cells express the transcription factor Mafb, and this marker can be used to highlight their posterior migration. They pass out between the anterior somite and the otic vesicle, before turning caudally and running along the base of the somites. With long-term fate mapping, we show that these cells migrate to the clavicle and settle at the site of formation of the attachment point for the cleidohyoid muscle. As such, the influence of the cranial neural crest in organising skeletomuscular connectivity seems to extend beyond the head into the trunk. These results are of further importance as they help explain how, even though the pectoral girdle and the skull became physically dissociated during tetrapod evolution, skeletomuscular connectivity has been maintained.
cis-acting translational effects of the 5' noncoding region of c-myc mRNA

We have previously shown that the 5' noncoding region of mouse c-myc mRNA has a negative effect on translational efficiency in a rabbit reticulocyte lysate (A. Darveau, J. Pelletier, and N. Sonenberg, Proc. Natl. Acad. Sci. USA 82:2315-2319, 1985). We wanted to localize and characterize the inhibitory translational element(s) in the mRNA and to study its effect in other in vitro and in vivo systems. Here we report that the restrictive element is confined to a 240-nucleotide sequence of the 5' noncoding region of mouse c-myc mRNA and that this sequence acts in cis to inhibit the translation of a heterologous mRNA. In addition, we report that the cis-inhibitory effect is also exhibited in microinjected Xenopus oocytes and wheat-germ extracts but not in HeLa cell extracts. Transfection of corresponding plasmid DNA constructs into several established cell lines did not produce the cis-inhibitory effect. A model to explain these results is presented.
Speed Up Learning based Descriptor for Face Verification

Many state of the art face recognition algorithms use local feature descriptors known as Local Binary Pattern (LBP). Many extensions of LBP exist, but the performance is still limited. Recently Learning Based Descriptor was introduced for face verification, it showed high discrimination power, but compared with LBP, it’s expensive to compute. In this paper, we propose a novel coding approach for Learning Based Descriptor (LE) descriptor which can keep the most discriminative LBP like feature as well as significantly shorten the feature extraction time. Since the proposed method speed up the LE descriptor’s feature extraction time, we call it Speeded Up Learning Descriptor or SULE for short. Tests on LFW standard benchmark show the superiority of SULE with respect of several state of the art feature descriptors regularly used in face verification
Enhanced recovery after surgery: Pain management

Effective pain management is fundamental to enhanced recovery after surgery. Selection of strategies should be tailored to patient and operation. As well as improving the quality of recovery, effective analgesia reduces the host stress response, facilitates mobilization and allows resumption of oral intake. Multi‐modal regimens combining paracetamol, non‐steroidal anti‐inflammatory agents where indicated, a potent opioid and a local anaesthetic technique achieve effective analgesia while limiting the dose and thereby side effects of any one agent.
Hox11‐family genes XHox11 and XHox11L2 in Xenopus: XHox11L2 expression is restricted to a subset of the primary sensory neurons

The mouse genome contains a small family of homeobox genes related to Hox11, but relatively little is known about the expression of these genes during early development. Hox11 itself is expressed in the embryonic spleen, among other tissues, and is required for its formation. No description of Hox11L2 expression has been presented previously. We have isolated the Xenopus orthologs of Hox11 and Hox11L2 and have carefully compared their expression patterns during embryogenesis. The localization of Xhox11 transcripts in the branchial arches, cranial sensory ganglia and spinal cord is similar, but not identical, to that of mouse Hox11. Xhox11 expression is not detected in the developing spleen. XHox11L2 is expressed exclusively in a portion of the primary sensory system in the frog embryo, including the cranial sensory ganglia and the Rohon‐Beard sensory neurons. There is significant overlap in the patterns of Xhox11 and XHox11L2 expression in the spinal cord and cranial sensory ganglia during early development, suggesting that they may function redundantly in these tissues. The timing of Xhox11 and Xhox11L2 expression indicates that Hox11‐family members may participate in the final stages of the differentiation process. Dev Dyn 1999;214:34–43. © 1999 Wiley‐Liss, Inc.
Prognostic implications of type 2 myocardial infarctions

Purpose: A type 2 myocardial infarction (MI) is due to ischemia from increased oxygen demand or decreased supply. It is not an infrequent complication following non-cardiac surgery or in the setting of acute medical illness. The purpose of this study was to determine whether type 2 MIs had a worse prognosis than a type 1 MI. Methods: The hospital database of a large terti-ary-level academic medical center was queried for all patients with a troponin I ≥ 1.6 ng/mL during a one-year period. The outcomes of patients with a type 2 MI were compared to those with a type 1 MI. Results: This retrospective study found that a type 2 MI is more lethal than the usual type 1 MI, particularly among women and the elderly. There was no statistical difference in outcomes between those that occurred in the post-operative setting compared to those that occurred in the setting of acute medical illness. Conclusions: Type 2 MIs are common and are associated with increased mortality, as compared with type 1 MIs. To date, no guidelines exist for the management of this type of MI. Further research into underlying mechanisms resulting in type 2 MI as well as potential treatment strategies is needed.
Study of novel Schmitt-PPI circuit structure

A kind of Schmitt-PPI circuit including Schmitt trigger and push-pull inverter with active resistor load is presented in this paper. It can get over the effect of Gauss white noise in the CMOS pulse circuit, and avoid the mistrigger to get relatively stable pulse. Through the simulation by EDA and the verification with the actual circuit, the structure gains ideal result.
The sociogenesis of psychological disorder: reexamining the causal issues with longitudinal data.

Although there is some consistency to the finding that socioeconomic status is related inversely to various measures and concepts of psychological disorder, the causal interpretation of this relationship remains uncertain. As usually stated, the issue is whether this inverse relationship arises from the fact and that low social status leads to disorder symptomatology (referred to as social causation) or a high degree of symptomatology impairs the individual's ability to be upwardly mobile (social selection). Research on this issue is far from definitive, especially for less severe types of symptomatology. The causal question is reformulated here in terms of the possible outcomes of a causal analysis of panel data. Based on data from two studies which each measure SES and psychological disorder symptomatology at multiple points in time, results from estimating a series of panel models incorporating unobserved variable specifications of both SES and disorder favor a social causation interpretation. However, differences in results across samples suggest that some modification of the simple social causation position may be necessary.
Information Behavior – Ein zentrales Forschungsthema der Informationswissenschaft

In diesem Beitrag wird die Beobachtung des Informationsverhaltens als zentraler methodischer Ansatz der informationswissenschaftlicher Forschung identifiziert, der auch unter Anerkennung terminologischer, evtl. sogar ontologischer Grundannahmen eine Kooperation über die Grenzen etablierter Paradigmata hinaus erlaubt. In this article we argue that information behavior can be seen as a central methodological approach to information science research, allowing cooperation beyond the scope of established paradigms in our discipline. In making our argument we introduce important terminological and ontological assumptions. Moreover, the text provides a frame of reference for the articles in the remainder of this special edition. Dans cet article, l’observation du comportement face à l’information est identifié comme une approche méthodologique centrale à la recherche dans le domaine des sciences de l’information, qui permet, même en reconnaissant les hypotheses terminologiques, voire ontologiques, la coopération au-delà des frontières des paradigmes établis.
Chain'width order and disorder in biopyriboles

High-resolution c-axis images of the biopyribole minerals anthophyllite [(Mg,Fe)' Si8Orr(OH)rl, jimthompsonite [(Mg,Fe),oSi,rOrr(OH)o], and chesterite [(Mg'Fe),rSiro O'4(OH)61 have been produced in the transmission electron microscope. Interpretation of the images has been confirmed by dynamical electron di-ffraction and imaging calculations. In material from Chester, Vermont, the chain width of areas corresponding to more than one million amphibole chains in the b direction has been imaged and examined. In addition to perfectly ordered phases, parts of some crystals exhibit chain-width disorder ranging from minor faulting of otherwise ordered material to extreme disorder in the chain sequence. Individual silicate chains with widths corresponding to as many as 333 pyroxene chains have been observed. Several new ordered mixed-chain pyriboles have been found to occur in limited but statistically signifrcant amounts. These new ordered structures have primitive unitcell chain sequences (2233), (233), (232233), (222333), (2332323), (2333), (433323), (2234), and (43332343332423). A method for distinguishing statistically-significant ordered structures from those that arise simply from random combination of structural elements is described. If the minerals chesterite and jimthompsonite are stable, their stability ranges are probably between those of anthophyllite and talc. The ordered minerals may, however, be produced metastably during reaction, as the result of constraints imposed by the reaction mechanisms. The occurrence of chesterite appears to be controlled by the chemical potentials of Fe, Ca, and Mg. The newly observed long-range ordered structures are probably the result of some non-random reaction or growth mechanism, rather than being thermodynamically stabilized.
Effects of Amoxicillin Subinhibitory Concentrations on the Cross-Protection Developed by Pneumococcal Antibodies in Mouse Sepsis Caused by an Amoxicillin-Resistant Serotype 6B Streptococcus pneumoniae Strain

ABSTRACT A model of mouse sepsis caused by a serotype 6B Streptococcus pneumoniae strain (amoxicillin MIC of 8 μg/ml) was developed to investigate the therapeutic effect of an amoxicillin dose (3.12 mg/kg of body weight three times daily for 48 h) producing, over the whole treatment period, subinhibitory concentrations in serum (peak concentration [Cmax]: 6.1 μg/ml) in animals that prior to infection had been passively immunized with a 6B or 23F hyperimmune serum (obtained by immunization with a whole-cell heat-inactivated inoculum and diluted to produce no protective effect by itself). Mortality in nonimmunized animals treated with antibiotic (3.12 mg/kg) was 90%, and mortality in animals immunized but not treated with the antibiotic was 100%. Antibiotic treatment in immunized animals produced mortality rates ≤20% when the hyperimmune serum was used, thus showing cross-protection and synergism (defined as the situation in which there is no response to the single agents [no differences versus placebo] while the combination exhibits significant activity) with subinhibitory concentrations of the antibiotic. The presence of antipneumococcal antibodies allowed antibiotic efficacy with negligible values of pharmacodynamic parameters (Cmax/MIC ratio of <1 and thus a null value for the time that serum levels exceed the MIC). This in vivo synergism offers a potential therapeutic strategy against resistant strains.
A vehicle-logo location approach based on edge detection and projection

Vehicle-logo location is an essential part of vehicle-logo recognition system. In this paper, a new method of vehicle-logo location is proposed. Firstly, the approximate region of vehicle-logo is obtained through the position of vehicle-plate. Then, we judge the texture of vehicle-logo background either horizontal or vertical with the edge detection and projection approach. Finally, with the known texture, position of the vehicle-logo can be located correctly by morphological filter and projection information. Experimental results demonstrate the proposed method is effective.