Miracle-dz/UniFAR-FTUs huggingface dataset:
Survival of Highly Pathogenic Avian Influenza H5N1 Virus in Tissues Derived from Experimentally Infected Chickens

ABSTRACT Eurasian lineage highly pathogenic avian influenza (HPAI) H5N1 virus has been a severe threat to the poultry industry since its emergence in 1996. The carcass or tissues derived from infected birds may present the risk of the virus spreading to humans, animals, and the surrounding environment. In this study, we investigated the survival of the virus in feather, muscle, and liver tissues collected from six chickens (Gallus gallus) experimentally infected with HPAI H5N1 virus. The tissues were stored at +4°C or +20°C, and viral isolation was performed at different times for 360 days. The maximum periods for viral survival were observed in samples stored at +4°C in all tissue types and were 240 days in feather tissues, 160 days in muscle, and 20 days in liver. The viral infectivity at +20°C was maintained for a maximum of 30 days in the feather tissues, 20 days in muscle, and 3 days in liver. The viral inactivation rates partly overlapped in the feather and muscle tissues at the two temperatures. The virus was inactivated rapidly in the liver. Our experimental results indicate that the tissue type and temperature can greatly influence the survival of HPAI H5N1 virus in the tissues of infected chickens. IMPORTANCE Highly pathogenic avian influenza virus of the H5N1 subtype can cause massive losses of poultry, and people need to handle a large number of chicken carcasses contaminated with the virus at outbreak sites. This study evaluated how long the virus can keep its infectivity in the three types of tissues derived from chickens infected with the virus. Our experimental results indicate that the virus can survive in tissues for a specific period of time depending on the tissue type and temperature. Our results are valuable for better understanding of viral ecology in the environment and for reducing the risk of the virus spreading via bird tissues contaminated with the virus.
How children’s rights are constructed in family-centred care

It appears that the acceptance of children’s rights within the acute care setting is treated as a given but such a given requires a more systematic analysis. This has been undertaken here in the form of a review of the literature. The purpose of the review is to explore how children’s rights, defined by the United Nations Convention on the Rights of the Child (UNCRC) are recognized in family-centred care in the acute care paediatric setting as reported in the literature. Reports that were available from 1989 to 2010 were reviewed. Children’s rights are not mentioned frequently in the literature of interest to children’s nurses. What is revealed are the ethical tensions in the challenge to act at all times in children’s best interests (in the spirit of Article 3) while giving due weight to their views (in the spirit of Article 12) (OHCHR, 1989). The continuing failure to address these tensions undermines the spirit and practice of family-centred care.
Fabrication and Characterization of a Wavenumber-Spiral Frequency-Steerable Acoustic Transducer for Source Localization in Plate Structures

This paper reports on the fabrication and the experimental characterization of a wavenumber frequency-steerable acoustic transducer (WS-FSAT). Here, the transducer is employed for the localization of broadband acoustic events corresponding to the propagation of guided elastic waves in an isotropic plate. The WS-FSAT records the plate response and defines the source location through a time-frequency analysis of the received signal. This is achieved by exploiting the frequency selective response of the transducer which directly maps the dominant component of the received signal to the direction of arrival of the incoming wave. This feature is the result of the spatial filtering effect produced by the characteristic shape of the sensing surface, which is designed in the wavenumber domain. Experiments are performed on a prototype fabricated on a polyvinylidene fluoride substrate mounted on an aluminum test plate. Tests are conducted for various source locations, and with multiple sources activated simultaneously. The results highlight the robustness of the proposed device, its good sensitivity and angular resolution, as well as the low complexity of hardware and signal processing. This paper suggests the WS-FSAT as an attractive solution for the detection of broadband acoustic events, such as impacts on structural substrates, and its potential use as part of active structural health monitoring systems based on pitch-catch or pulse-echo operations.
An Adaptive Method for Selecting Question Pools Using C4.5

A number of adaptive mechanisms for asking questions have been proposed. This paper presents a novel adaptive method for selecting questions from various pools of questions. The method uses a state-diagram to represent various question pools where transitions between the states are dynamically modified to generate the optimal pool of questions for the next question. C4.5 is used to close the loop on adaptive selection of questions. A case study showing how the method was implemented for an online environmental game is presented and evaluated.
Prevalence of ColE1-Like Plasmids and Kanamycin Resistance Genes in Salmonella enterica Serovars

ABSTRACT Multi-antimicrobial-resistant Salmonella enterica strains frequently carry resistance genes on plasmids. Recent studies focus heavily on large conjugative plasmids, and the role that small plasmids play in resistance gene transfer is largely unknown. To expand our previous studies in assessing the prevalence of the isolates harboring ColE1-like plasmids carrying the aph gene responsible for kanamycin resistance (Kanr) phenotypes, 102 KanrSalmonella isolates collected through the National Antimicrobial Resistance Monitoring System (NARMS) in 2005 were screened by PCR using ColE1 primer sets. Thirty isolates were found to be positive for ColE1-like replicon. Plasmids from 23 isolates were able to propagate in Escherichia coli and were subjected to further characterization. Restriction mapping revealed three major plasmid groups found in three or more isolates, with each group consisting of two to three subtypes. The aph genes from the KanrSalmonella isolates were amplified by PCR, sequenced, and showed four different aph(3′)-I genes. The distribution of the ColE1 plasmid groups in association with the aph gene, Salmonella serovar, and isolate source demonstrated a strong linkage of the plasmid with S. enterica serovar Typhimurium DT104. Due to their high copy number and mobility, the ColE1-like plasmids may play a critical role in transmission of antibiotic resistance genes among enteric pathogens, and these findings warrant a close monitoring of this plasmid incompatibility group.
Evidence for Coexistence of Distinct Escherichia coli Populations in Various Aquatic Environments and Their Survival in Estuary Water

ABSTRACT Escherichia coli, a commensal bacterium from the intestinal tracts of humans and vertebrate animals, has been used as one of two bacterial indicators of fecal contamination, along with intestinal enterococci, to monitor the microbiological quality of water. However, water environments are now recognized as a secondary habitat where some strains can survive. We investigated the survival of E. coli isolates collected from bodies of water in France exhibiting distinct profiles of contamination, defined according to the following criteria: vicinity of the point sources of contamination, land use, hydrology, and physicochemical characteristics of the receiving water. We selected 88 E. coli strains among a collection of 352 strains to carry out a microcosm experiment in filtered estuarine water for 14 days at 10°C. The relationship between the survival of E. coli strains and genotypic and phenotypic characteristics was analyzed. This work showed that distinct E. coli survival types, able to survive from between 7 and 14 days to less than 2 days, coexisted in the water. E. coli isolates that rapidly lost their culturability were more frequently isolated in water recently contaminated by fecal bacteria of human origin, and most were multiresistant to antibiotics and harbored several virulence factors. In contrast, persistent strains able to survive from 4 to 14 days were more often found in water with low levels of fecal bacteria, belonged mainly to the B1 phylogroup, often harbored only one virulence factor, kspE or ompT, and were able to grow at 7°C.
Genome Sequence of Thermotolerant Bacillus methanolicus: Features and Regulation Related to Methylotrophy and Production of l-Lysine and l-Glutamate from Methanol

ABSTRACT Bacillus methanolicus can utilize methanol as its sole carbon and energy source, and the scientific interest in this thermotolerant bacterium has focused largely on exploring its potential as a biocatalyst for the conversion of methanol into l-lysine and l-glutamate. We present here the genome sequences of the important B. methanolicus model strain MGA3 (ATCC 53907) and the alternative wild-type strain PB1 (NCIMB13113). The physiological diversity of these two strains was demonstrated by a comparative fed-batch methanol cultivation displaying highly different methanol consumption and respiration profiles, as well as major differences in their l-glutamate production levels (406 mmol liter−1 and 11 mmol liter−1, respectively). Both genomes are small (ca 3.4 Mbp) compared to those of other related bacilli, and MGA3 has two plasmids (pBM19 and pBM69), while PB1 has only one (pBM20). In particular, we focus here on genes representing biochemical pathways for methanol oxidation and concomitant formaldehyde assimilation and dissimilation, the important phosphoenol pyruvate/pyruvate anaplerotic node, the tricarboxylic acid cycle including the glyoxylate pathway, and the biosynthetic pathways for l-lysine and l-glutamate. Several unique findings were made, including the discovery of three different methanol dehydrogenase genes in each of the two B. methanolicus strains, and the genomic analyses were accompanied by gene expression studies. Our results provide new insight into a number of peculiar physiological and metabolic traits of B. methanolicus and open up possibilities for system-level metabolic engineering of this bacterium for the production of amino acids and other useful compounds from methanol.
The effect of temperature on mortality in Stockholm 1998—2003: A study of lag structures and heatwave effects

Aims: To describe seasonal patterns of natural mortality in Stockholm as well as the temperature—mortality relationship and the lag structure for effects of high and low temperatures; to describe the impact of high temperatures on cardiovascular and respiratory mortality, and the general effect of high temperatures in different age groups; and to investigate whether there is any indication of an additional heatwave or cold spell effect. Methods: Generalized additive Poisson regression models were fitted to mortality and temperature data from Stockholm from the period 1998—2003, controlling for influenza, season, time trends, week day, and holidays. Results: The mortality in Stockholm followed a seasonal pattern, with a peak in the winter season. The ``optimal temperature'' was around 11—12°C. Above this temperature, the cumulative general relative risk (RR) corresponded to a 1.4% (95% confidence interval (CI)=0.8—2.0) increase per °C, and below this temperature the cumulative RR corresponded to a 0.7% (95% CI=0.5—0.9) decrease per °C. Age-specific RRS were estimated above the threshold for age <65 years, age 65—74 years, and age >74 years, with estimated increases of 0.5% (not significant), 1.5% (not significant) and 1.6% (95% CI=0.9—2.3) per °C, respectively. The RRs for cardiovascular and respiratory causes were studied above the breakpoint, and estimated to be 1.1% (95% CI=0.3—2.0) and 4.3% (95% CI=2.2—6.5) per °C, respectively. The lag structures from moving averages and polynomial distributed lag models coincided with a rather direct effect during summer (lag 0 and 1) and a more prolonged effect during winter, covering about a week. The inclusion of an indicator of heatwaves added an increase in daily mortality of 3.1—7.7%, depending on the threshold. Conclusions: These results show that the predicted increase in heat events must also be taken seriously in Scandinavia, whatever the extent of the decreasing cold related mortality. The relative risks associated with heat and heatwaves seem stronger than the cold effects and thus a larger public health threat, since northern populations have not yet adapted to heat as have been done over a long time for the cold periods. The pressure on the healthcare sector will probably increase in the warm season, periodically it may become even greater than the pressure due to cold weather, which will be a new phenomenon for the healthcare sector to cope with. We need to be prepared for these kind of events by developing adaptation and education strategies to handle the consequences that a warmer climate will have for public health and the healthcare sector.
Job stress, sickness absence and sickness presenteeism in Nordic elderly care

Aims: Nordic elderly care has been restructured to obtain more efficiency. Among workers caring for the elderly, levels of perceived job stress could vary, due to understaffing and resource scarcity. This study examines how sickness absence and sickness presenteeism are associated with perceived job stress. Methods: Data were obtained by posting questionnaires to lower-level care staff in Sweden (n=483), Denmark (n=704), Finland (n=597), and Norway (n=663). Self-reports about job stress (four items), sickness absence and sickness presenteeism were analysed by cross-tabulations and logistic regression. Results: Each item of job stress was associated with sickness absence and sickness presenteeism in the samples from all four countries. With low levels of job stress, levels of reported sickness absence and sickness presenteeism were relatively moderate. With increasing levels of job stress, the level of sickness presenteeism rose more sharply than that of sickness absence. Conclusions: The results can be interpreted in the light of features inherent in care work. Owing to professional norms and moral obligations, care workers could lower their thresholds for taking sick leave when care organizations are understaffed, because absences will be particularly critical for care recipients in such circumstances. Thus, while increasing job stress tends to be accompanied both by more sickness absence and by more sickness presenteeism, sickness presenteeism rises particularly in cases of high levels of job stress. Owing to cross-sectional data and self-reported information, conclusions are tentative.
Unusual Butane- and Pentanetriol-Based Tetraether Lipids in Methanomassiliicoccus luminyensis, a Representative of the Seventh Order of Methanogens

ABSTRACT A new clade of archaea has recently been proposed to constitute the seventh methanogenic order, the Methanomassiliicoccales, which is related to the Thermoplasmatales and the uncultivated archaeal clades deep-sea hydrothermal vent Euryarchaeota group 2 and marine group II Euryarchaeota but only distantly related to other methanogens. In this study, we investigated the membrane lipid composition of Methanomassiliicoccus luminyensis, the sole cultured representative of this seventh order. The lipid inventory of M. luminyensis comprises a unique assemblage of novel lipids as well as lipids otherwise typical for thermophilic, methanogenic, or halophilic archaea. For instance, glycerol sesterpanyl-phytanyl diether core lipids found mainly in halophilic archaea were detected, and so were compounds bearing either heptose or methoxylated glycosidic head groups, neither of which have been reported so far for other archaea. The absence of quinones or methanophenazines is consistent with a biochemistry of methanogenesis different from that of the methanophenazine-containing methylotrophic methanogens. The most distinctive characteristic of the membrane lipid composition of M. luminyensis, however, is the presence of tetraether lipids in which one glycerol backbone is replaced by either butane- or pentanetriol, i.e., lipids recently discovered in marine sediments. Butanetriol dibiphytanyl glycerol tetraether (BDGT) constitutes the most abundant core lipid type (>50% relative abundance) in M. luminyensis. We have thus identified a source for these unusual orphan lipids. The complementary analysis of diverse marine sediment samples showed that BDGTs are widespread in anoxic layers, suggesting an environmental significance of Methanomassiliicoccales and/or related BDGT producers beyond gastrointestinal tracts. IMPORTANCE Cellular membranes of members of all three domains of life, Archaea, Bacteria, and Eukarya, are largely formed by lipids in which glycerol serves as backbone for the hydrophobic alkyl chains. Recently, however, archaeal tetraether lipids with either butanetriol or pentanetriol as a backbone were identified in marine sediments and attributed to uncultured sediment-dwelling archaea. Here we show that the butanetriol-based dibiphytanyl tetraethers constitute the major lipids in Methanomassiliicoccus luminyensis, currently the only isolate of the novel seventh order of methanogens. Given the absence of these lipids in a large set of archaeal isolates, these compounds may be diagnostic for the Methanomassiliicoccales and/or closely related archaea.
Quantifying Vibrio cholerae Enterotoxicity in a Zebrafish Infection Model

ABSTRACT Vibrio cholerae is the etiological agent of cholera, an acute intestinal infection in humans characterized by voluminous watery diarrhea. Cholera is spread through ingestion of contaminated food or water, primarily in developing countries that lack the proper infrastructure for proper water and sewage treatment. Vibrio cholerae is an aquatic bacterium that inhabits coastal and estuarine areas, and it is known to have several environmental reservoirs, including fish. Our laboratory has recently described the use of the zebrafish as a new animal model for the study of V. cholerae intestinal colonization, pathogenesis, and transmission. As early as 6 h after exposure to V. cholerae, zebrafish develop diarrhea. Prior work in our laboratory has shown that this is not due to the action of cholera toxin. We hypothesize that accessory toxins produced by V. cholerae are the cause of diarrhea in infected zebrafish. In order to assess the effects of accessory toxins in the zebrafish, it was necessary to develop a method of quantifying diarrheal volume as a measure of pathogenesis. Here, we have adapted cell density, protein, and mucin assays, along with enumeration of V. cholerae in the zebrafish intestinal tract and in the infection water, to achieve this goal. Combined, these assays should help us determine which toxins have the greatest diarrheagenic effect in fish and, consequently, which toxins may play a role in environmental transmission. IMPORTANCE Identification of the accessory toxins that cause diarrhea in zebrafish can help us understand more about the role of fish in the wild as aquatic reservoirs for V. cholerae. It is plausible that accessory toxins can act to prolong colonization and subsequent shedding of V. cholerae back into the environment, thus perpetuating and facilitating transmission during an outbreak. It is also possible that accessory toxins help to maintain low levels of intestinal colonization in fish, giving V. cholerae an advantage when environmental conditions are not optimal for survival in the water. Studies such as this one are critical because fish could be an overlooked source of cholera transmission in the environment.
L’expectoration induite, outil de diagnostic de l’asthme professionnel

Le diagnostic de l’asthme professionnel (AP) repose essentiellement sur la mise en evidence de changements fonctionnels respiratoires consecutifs a une exposition a des agents presents en milieu de travail. L’expectoration induite permet d’evaluer de facon repetee l’inflammation bronchique de la plupart des sujets victimes d’un AP. Ceux-ci presentent, pour la plupart, une eosinophilie bronchique lors de l’exposition a l’agent causal de leur AP. Cette eosinophilie disparait ou diminue quand les sujets ne sont plus exposes a l’agent qui induit l’AP. Elle se manifeste egalement lorsque ces patients sont exposes en laboratoire a l’agent professionnel en cause. La persistance d’une inflammation bronchique chez les sujets atteints d’AP, que l’on a soustraits a l’agent a l’origine de leur maladie, pourrait etre un facteur de mauvais pronostic. Le recours a l’expectoration induite est, a notre point de vue, un outil utile dans le diagnostic et le suivi de l’AP. Son role dans la surveillance de travailleurs a risque d’etre victime de l’AP reste cependant a etre determine.
An active filter for Delta-Sigma modulated Josephson waveforms

We present an active low pass filter constructed for Delta-Sigma modulated Josephson waveforms. The filter has a fifth order Butterworth characteristic with high input impedance and stable passband performance, including dc. We present results from frequency response, input impedance characteristics, passband ac-dc measurements and spectra of a Delta-Sigma modulated sine wave from a programmable Josephson voltage standard.
DDS-Based Signal-Generation Architecture Comparison for an Imaging Radar at 300 GHz

Submillimeter-wave imaging radars for standoff detection require broadband and fast-switch signal-generation architectures in order to achieve a high-range-resolution and near-video-rate imaging system. The signal generation has a strong influence on the radar image quality, primarily due to the transmitted phase noise. Two direct digital synthesis-based architectures for continuous-wave linear-frequency-modulated signal generation have been fabricated and tested for a high-range-resolution imaging radar at 300 GHz for standoff sensing of person-borne concealed threats. In order to select the signal-generation architecture that ensures the proper operation of the imaging radar with a cost-saving objective, three figures of merit have been used: 1) radar image quality; 2) power consumption; and 3) cost. The impact of the signal-generation architectures on the imaging radar indicates that both architectures present a similar radar performance in terms of radar image quality, although the narrowband direct-digital-synthesis/phase-locked loop scheme is a cost-effective solution compared with the broadband direct-digital-synthesis scheme in order to develop an affordable and energy-efficient high-performance preindustrial radar prototype.