Miracle-dz/UniFAR-FTUs huggingface dataset:
Co-localization of Endogenous and Exogenous p53 Proteins in Nasopharyngeal Carcinoma Cells

Recently, we have established nine nasopharyngeal carcinoma (NPC) cell lines in which only one cell line showed the p53 mutation. For investigation of the p53 mutation in this line, immunostaining using anti-p53 antibody was applied and showed the presence of p53 protein in the cytoplasm but not in the nucleus. Single strand conformation polymorphism analysis of the p53 gene showed one normal and one additional DNA band. Cloning and sequencing of PCR-amplified DNA showed an AGA (arginine) to ACA (threonine) heterozygous point mutation at codon 280. Transfection of the p53 DNA binding sequence and chloramphenicol acetyltransferase assay revealed loss of transcriptional activation function of endogenous p53 protein. Co-localization of the endogenous and the transfected exogenous p53 protein by polyclonal antibodies to anti-p53 protein revealed strong exogenous p53 staining in the transfected nuclei and weak staining of endogenous p53 protein in the cytoplasm. We concluded that (a) a heterozygous point mutation at codon 280 was identified in the NPC-TW 06 cell line; (b) the point mutation may cause the stagnation of mutant p53 protein in the cytoplasm, and loss of its transcriptional activation function; (c) endogenous and exogenous p53 protein can be co-localized at the same time in the transfected cells; and (d) 280 mutant p53 protein in NPC cells does not cause a decrease or increase in sensitivity to chemotherapy.
A Survey of Challenges in selection of Vehicular Mobility Models in VANET

With the recent advancement of technology, a new technology named VANET (Vehicular Adhoc Network) is emerging day by day. VANET is a wireless communication between vehicles to vehicles and RSU (vehicles to road side units). It is different from MANET, so the challenges of VANET are also different from MANET. It has many challenges like safety, traffic and user application based challenges which require some particular design. The vehicular mobility model plays a vital role in examining different challenges. There are different models for different purposes and for getting better results we have to apply the correct model which is suitable for the particular situation. In this paper, a proper classification is done between different vehicular mobility models with respect to their types, sub types, usage (interaction level), evaluating purpose and example of each model is also provided.
Security-aware elasticity for NoSQL databases in multi-cloud environments

We focus on horizontally scaling NoSQL databases in a cloud environment, in order to meet performance requirements while respecting security constraints. The performance requirements refer to strict latency limits on the query response time. The security requirements are derived from the need to address two specific kinds of threats that exist in cloud databases, namely data leakage, mainly due to malicious activities of actors hosted on the same physical machine, and data loss after one or more node failures. A key feature of our approach is that we account for multiple cloud providers offering resources of different characteristics. We explain that usually there is a trade-off between performance and security requirements and we derive a model checking approach to drive runtime decisions that strike a user-defined balance between them taking into account the infrastructure heterogeneity. Finally, we evaluate our proposal using real traces to prove the effectiveness in configuring the trade-offs.
Inhibition of sulfathiazole crystal growth by polyvinylpyrrolidone.

Abstract The results of polyvinylpyrrolidone inhibition of sulfathiazole single crystal growth are presented. It was found that the minimum concentration of polyvinylpyrrolidone required to inhibit completely crystal growth was a linear function of the suprsaturation ratio with an intercept of ~1.15 exhibited on the latter axis. It was also found that the minimum concentration of polyvinylpyrrolidone required for complete inhibition of crystal growth was a function of the molecular weight of polyvinylpyrrolidone used for the inhibition study. The results of inhibition studies using molecular weights of polyvinylpyrrolidone of 10,000, 40,000, and 360,000 are presented. The data suggest that the inhibition point depends on the relative rates of transport of polyvinylpyrrolidone and sulfathiazole to the crystal surface from the bulk of the solution. A model is presented that appears to be consistent with the data.
Modeling the Impact of Trust and Distrust in Agent Networks

Recently, the role of trust in agent networks has received a lot of attention. In his paper, we argue that an explicit consideration of distrust and its complex interaction with individual trust and confidence in the network as a whole is equally important, especially when designing hybrid networks of human and machine agents. We propose a trust-confidence-distrust (TCD) model of agent network dynamics, and present a multi-perspective methodology according to which such networks can be modeled and simulated. We are currently validating this methodology in the context of designing specialized computer support for networks of organizations, e.g. in the context of high-tech entrepreneurship or continuing education.
Making nursing work: breaking through the role confusion of advanced practice nursing.

AIM This paper reports a study to develop a research-informed model of the service parameters and an analysis framework for advanced practice nursing roles. BACKGROUND Changing patterns of health care are forcing service planners to examine new service delivery models. Apparent is the call for nursing service that incorporates expanded levels of autonomy, skill and decision-making. A number of nursing roles conform to this description under the generic title of advanced practice nurse. However, there is confusion in the health service community internationally about nomenclature, role and scope for advanced practice nursing roles. An emerging priority in response to recent developments in the nurse practitioner role is to establish service parameters for advanced practice nursing and to differentiate operationally between advanced practice and practitioner nursing roles. METHODS We conducted an interpretive, qualitative examination of the practice of a random sample of nine advanced practice nurses working in three acute care hospitals in south east Queensland, Australia in 2006. Data collection involved individual in-depth interviews, which were deductively analysed and tested against published advanced practice nursing models. RESULTS The data most comprehensively supported the Strong Model of Advanced Practice as representing the practice experiences of the research participants. This model supports definition of the service parameters and the design of an operational framework for implementation and evaluation of advanced practice nursing roles. CONCLUSION The findings differentiate advanced practice nurse and nurse practitioner roles, and offer an operational framework to identify, establish and evaluate advanced and extended nursing positions. Subject to further validation, this outcome can provide operational information for implementing innovative nursing roles appropriate to consumer needs and specific health service models.
Probiotics in Larvae and Juvenile Whiteleg Shrimp Litopenaeus vannamei

In penaeid shrimps, Vibrio spp. is the main cause of bacterial diseases, such as V. parahaemolyticus, V. alginolyticus, V. harveyi (Garriques and Arevalo, 1995) and V. penaeicida (Aguirre-Guzman and Ascencio-Valle, 2001). Possible mode of infection consists of three basic steps: (i) the bacterium penetrates the host cuticle or exoskeleton wound by means of chemotactic motility; (2) within the host tissues the bacterium deploys iron-sequestering systems; e.g., sidero-phores, to “steal” iron from the host; and (3) the bacterium eventually damages the organisms by means of extracellular products, e.g. hemolysins and proteases (Thompson et al., 2004). Containing high loads of either Vibrio parahaemolyticus or V. harveyi induced the rounding up and detachment of epithelial cells from the basal lamina of the midgut trunk. Epithelial cell detachment of epithelial was not seen in the presence of nonpathogenic bacteria (probiotics) (Chen et al., 2000; Martin et al., 2004). Pathogens like Vibrio spp. , which cause detachment of the epithelium in the midgut trunk, can affect high mortality in shrimp by eliminating 2 layers that protect the shrimp from infections: the epithelium and the peritrophic membrane it secretes. In addition, loss of the epithelium may affect the regulation of water and ion outtake into the body (Mykles 1977, Neufeld and Cameron 1994).
Model predicting survival in stage I melanoma based on tumor progression.

We used the lesional steps in tumor progression and multivariable logistic regression to develop a prognostic model for primary, clinical stage I cutaneous melanoma. This model is 89% accurate in predicting survival. Using histologic criteria, we assigned melanomas to tumor progression steps by ascertaining their particular growth phase. These phases were the in situ and invasive radial growth phase and the vertical growth phase (the focal formation of a dermal tumor nodule or dermal tumor plaque within the radial growth phase or such dermal growth without an evident radial growth phase). After a minimum follow-up of 100.6 months and a median follow-up of 150.2 months, 122 invasive radial-growth-phase tumors were found to be without metastases. Eight-year survival among the 264 patients whose tumors had entered the vertical growth phase was 71.2%. Survival prediction in these patients was enhanced by the use of a multivariable logistic regression model. Twenty-three attributes were tested for entry into this model. Six had independently predictive prognostic information: (a) mitotic rate per square millimeter, (b) tumor-infiltrating lymphocytes, (c) tumor thickness, (d) anatomic site of primary melanoma, (e) sex of the patient, and (f) histologic regression. When mitotic rate per square millimeter, tumor-infiltrating lymphocytes, primary site, sex, and histologic regression are added to a logistic regression model containing tumor thickness alone, they are independent predictors of 8-year survival (P less than .0005).
Dual-band coupling and feed structure for microstrip filter design

A new configuration of dual-band filter design is presented in this paper. Stepped-impedance transmission-line sections are used as the tunable dual-band resonators. New coupling structures and matching networks at input and output are also proposed to achieve a dual-band characteristics. Three filters with their second passband at 1.5, 2, and 2.5 times the fundamental frequency are designed as examples, and they are all verified by experimental results.
Reduction of Dexamethasone-Induced Oxidative Stress and Lipid Peroxidation in Laying Hens by Dietary Vitamin E Supplementation

The objective of the present study is to research the effect of dietary vitamin E (Vit E) on laying performance, lipid peroxidation and antioxidative status in laying hens under experimentally induced oxidative stress conditions via Dexamethasone (DEX) administration. A total of 60 Egyptian local strain (Gimmizah) laying hens at 36 weeks of age were housed in individual cages in an open-sided building under a 16 hr light: 8 hr dark lighting schedule. The birds were randomly divided into four experimentally treatments: DEX (4 mg/hen/day); Vit. E (200 mg/kg diet); DEX+Vit. E (4 mg/hen/day+ 200 mg/kg diet respectively); and control (n= 15). The birds were provided with commercial feed and water ad libitum. All treatments lasted for 7 successive days. Oxidative stress induced by DEX injections significantly reduced egg production and egg weight. Vit. E alleviated this reduction when supplemented to the stress-induced hens, compared to DEX treatment. A high level of yolk lipid oxidation was associated with oxidative stress treatment and it was reduced by Vit E supplementation. It could be concluded that super- nutritional levels of Vit E (200 mg/kg diet) may contribute in enhancing laying performance and antioxidative status of laying hens under stress conditions.
From minichaperone to GroEL 3: properties of an active single-ring mutant of GroEL.

The next step in our reductional analysis of GroEL was to study the activity of an isolated single seven-membered ring of the 14-mer. A known single-ring mutant, GroEL(SR1), contains four point mutations that prevent the formation of double-rings. That heptameric complex is functionally inactive because it is unable to release GroES. We found that the mutation E191G, which is responsible for the temperature sensitive (ts) Escherichia coli allele groEL44 and is located in the hinge region between the intermediate and apical domains of GroEL, appears to function by weakening the binding of GroES, without destabilizing the overall structure of GroEL44 mutant. We introduced, therefore, the mutation E191G into GroEL(SR1) in order to generate a single-ring mutant that may have weaker binding of GroES and hence be active. The new single-ring mutant, GroEL(SR44), was indeed effective in refolding both heat and dithiothreitol-denatured mitochondrial malate dehydrogenase with great efficiency. Further, unlike all smaller constructs of GroEL, the expression of GroEL(SR44) in E. coli that contained no endogenous GroEL restored biological viability, but not as efficiently as does wild-type GroEL. We envisage the notional evolution of the structure and properties of GroEL. The minichaperone core acts as a primitive chaperone by providing a binding surface for denatured states that prevents their self-aggregation. The assembly of seven minichaperones into a ring then enhances substrate binding by introducing avidity. The acquisition of binding sites for ATP then allows the modulation of substrate binding by introducing the allosteric mechanism that causes cycling between strong and weak binding sites. This is accompanied by the acquisition by the heptamer of the binding of GroES, which functions as a lid to the central cavity and competes for peptide binding sites. Finally, dimerization of the heptamer enhances its biological activity.
The histological diagnosis of leprosy type 1 reactions: identification of key variables and an analysis of the process of histological diagnosis

Background: Type 1 leprosy reactions (T1R) are a major inflammatory complication of leprosy affecting 30% of patients with borderline leprosy, but there has been no diagnostic evaluation of the histological diagnosis of this entity. Methods: In a prospective study based in India, skin biopsies were taken from 99 patients with clinically diagnosed T1R and 52 non-reactional controls. These were assessed histologically by four histopathologists whose assessments were then compared. Results: Reactions were under-diagnosed, with 32–62% of clinically diagnosed reactions being given a histological diagnosis. The pathologists showed good specificities (range 72% to 93%) but much poorer sensitivities (range 42% to 78%). The most commonly reported histological features of TIR were cell maturity, oedema and giant cells. Five key variables were identified that the pathologists used in diagnosing a reaction: intra-granuloma oedema, giant cell size, giant cell numbers, dermal oedema and HLA-DR expression. A predictive model for the diagnosis of T1R was developed using stepwise logistic regression analysis, with clinical diagnosis of reaction as an outcome, and then identification of the key variables that each pathologist used in making the diagnosis of T1R. 34–53% of the variation between pathologists could be accounted for. The four pathologists used a similar diagnostic model and for all of them their estimations of epithelioid cell granuloma oedema, dermal oedema, plasma cells and granuloma fraction were significant variables in the diagnosis of T1R. Each pathologist then added in variables that were specific to themselves. Conclusions: This study has identified T1R as being under-diagnosed in comparison with clinical assessments. Key variables for diagnosing T1R were established. This comparative masked study highlights the need for such studies in other inflammatory conditions.
Modelling and Control Design for a Hydraulic Forestry

In the industry of forestry cranes, the design of automated machines that improve productivity has been a challenge for many researchers and engineers in the field of Control Systems and Robotics. An appropriate control design requires a detailed knowledge of the physical phenomena involved in the real system. At this point it is needed to perform simulation tests more rapid than experimental ones. In order to run those simulations, a model of the real machine is required. In this project the MATLAB/Simulink package for both modelling and simulation has been used. Basic mathematical models of components involved in the system as well as the final block diagrams are presented in this report. In heavy-duty machinery, friction may reduce the performance significantly and negative side effects such as tracking errors, large settling times or limit cycles are usually introduced. In todays high-performance motion systems such effects have to be taken into account in the control design in order to gain accuracy. For this reason certain types of tests must be performed to understand and interpret the friction phenomena hidden in the system. The identification of friction parameters based on experiments is explained in this report and a corresponding model for the system is presented. The derived control system is based on an empirical tuned PID controller with an additional feedforward term for friction compensation. Generally, friction models require the velocity as input. Due to the fact that only positions are measured, an elaborate estimation algorithm is proposed. As result of this report, two examples of an autonomous motion are presented and an outlook on further improvements is given. A comparison of the derived simulation model with the behaviour of the real machine is also shown.
A comparison of two ART-based neural networks for hierarchical clustering

The paper compares two modular neural network architectures, built up of adaptive resonance theory (ART) networks, that can develop stable two-level hierarchical clusterings of arbitrary sequences of binary input patterns. In particular, it contrasts the typical class hierarchies that the networks found on a machine learning benchmark database. It is proposed that the main difference between the two clusterings are the direct consequence of the existence or absence of an internal feedback mechanism and explicit associative links between a higher-level class and its sub-classes.
An Unusual Case of Cephalocele Associated with Lipoma of Corpus Callosum

Cephalocele is a part of the family of neural tube defects. Although the frequency has ranged from 1 per 2,400 to 1 per 12,500 live births, the true incidence has been hidden in stillborns, underreferral of massive defects and early pregnancy losses. It has been suggested that the size, the content of the sac and associated hydrocephalus were unfavorable factors for the prognosis. Cephaloceles may occur as isolated malformations or together with other anomalies, and associated abnormalities are present in up to 50% of the cases. We report an unusual case of encephalocele associated with lipoma of corpus callosum, and the features of magnetic resonance imaging are discussed.
Nevus count associations with pigmentary phenotype, histopathological melanoma characteristics and survival from melanoma

Although nevus count is an established risk factor for melanoma, relationships between nevus number and patient and tumor characteristics have not been well studied and the influence of nevus count on melanoma‐specific survival is equivocal. Using data from the Genes, Environment and Melanoma (GEM) study, a large population‐based study of primary cutaneous melanoma, we evaluated associations between number of nevi and patient features, including sun‐sensitivity summarized in a phenotypic index, and tumor characteristics. We also assessed the association of nevus count with melanoma‐specific survival. Higher nevus counts were independently and positively associated with male gender and younger age at diagnosis, and they were inversely associated with lentigo maligna histology. We observed a borderline significant trend of poorer melanoma‐specific survival with increasing quartile of nevus count, but little or no association between number of nevi and pigmentary phenotypic characteristics or prognostic tumor features.
A novel phospholipase C delta4 (PLCdelta4) splice variant as a negative regulator of PLC.

It has been reported that there are two alternatively spliced variants of phospholipase C-delta4 (PLCdelta4), termed ALT I and II, that contain an additional 32 and 14 amino acids in their respective sequences in the linker region between the catalytic X and Y domains (Lee, S. B., and Rhee, S. G. (1996) J. Biol. Chem. 271, 25-31). We report here the isolation and characterization of a novel alternative splicing isoform of PLCdelta4, termed ALT III, as a negative regulator of PLC. In ALT III, alternative splicing occurred in the catalytic X domain, i.e. 63 amino acids (residues 424-486) containing the C-terminal of the X domain and linker region were substituted for 32 amino acids corresponding to the insert sequence of ALT I. Although the expression level of ALT III was found to be much lower in most tissues and cells compared with that of PLCdelta4, it was significantly higher in some neural cells, such as NIE-115 cells and p19 cells differentiated to neural cells by retinoic acid. Interestingly, recombinant ALT III protein did not retain enzymatic activity, and the activity of PLCdelta4 overexpressed in COS7 cells was markedly decreased by the co-expression of ALT III but not by ALT I or II. Moreover, N-terminal pleckstrin homology domain (PH domain) of ALT III alone could inhibit the increase of inositol-1,4, 5-trisphosphate levels in PLCdelta4-overexpressing NIH3T3 cells, whereas a PH domain deletion mutant could not, indicating that the PH domain is necessary and sufficient for its inhibitory effect. The ALT III PH domain specifically bound to phosphatidylinositol (PtdIns)-4,5-P2 and PtdIns-3,4,5-P3 but not PtdIns, PtdIns-4-P, or inositol phosphates, and the mutant R36G, which retained only weak affinity for PtdIns-4,5-P2, could not inhibit the activity of PLCdelta4. These results indicate that PtdIns-4,5-P2 binding to PH domain is essential for the inhibitory effect of ALT III. ALT III also inhibited PLCdelta1 activity and partially suppressed PLCgamma1 activity, but not PLCbeta1 in vitro; it did inhibit all types of isozymes tested in vivo. Taken together, our results indicate that ALT III is a negative regulator of PLC that is most effective against the PLC delta-type isozymes, and its PH domain is essential for its function.