Miracle-dz/UniFAR-FTUs huggingface dataset:
Examining body dissatisfaction in college men through the exploration of appearance anxiety and internalization of the mesomorphic ideal

Abstract Although research has explained various psychological factors leading to body dissatisfaction in male, limited research has examined the potential mediating effect of the anxiety male experience to align with societal conceptions of masculine appearance. Objective and participants: In this study, conducted spring semester of 2018, we examined the relationships between internalization of the mesomorphic ideal, appearance anxiety, and body dissatisfaction, as well as the potential mediating effect of appearance anxiety on body dissatisfaction in college-aged male. Methods: In a convenience sample of college-aged male, data were collected using self-reported questionnaires measuring internalization, appearance anxiety, and body dissatisfaction. Results: Responses indicated significant associations between internalization of the mesomorphic ideal, appearance anxiety, and body dissatisfaction. Appearance anxiety was a partial mediator of internalization of the mesomorphic ideal and body dissatisfaction. Conclusions: The authors discuss implications for future research and address factors related to body dissatisfaction for professionals implementing body image programs.
Dynamic Competition of Polycomb and Trithorax in Transcriptional Programming.

Predicting regulatory potential from primary DNA sequences or transcription factor binding patterns is not possible. However, the annotation of the genome by chromatin proteins, histone modifications, and differential compaction is largely sufficient to reveal the locations of genes and their differential activity states. The Polycomb Group (PcG) and Trithorax Group (TrxG) proteins are the central players in this cell type-specific chromatin organization. PcG function was originally viewed as being solely repressive and irreversible, as observed at the homeotic loci in flies and mammals. However, it is now clear that modular and reversible PcG function is essential at most developmental genes. Focusing mainly on recent advances, we review evidence for how PcG and TrxG patterns change dynamically during cell type transitions. The ability to implement cell type-specific transcriptional programming with exquisite fidelity is essential for normal development. Expected final online publication date for the Annual Review of Biochemistry, Volume 89 is June 22, 2020. Please see http://www.annualreviews. org/page/journal/pubdates for revised estimates.
Discussion on The Relationship of Mitochondrion and Qi

To explain the connotation of Qi in TCM by modern medical theory and reveal the essence of it plays an important part in the modernization of TCM.With the knowledge of the realization of Qi by TCM and modern medicine,the article compared the theory of Qi in TCM with the energy metabolism of mitochondrion and the result shows that they have many close relations.The conclusion of the study suggests that the mitochondrion would be an important part of the Qi .
Youth Vaping and Tobacco Use in Context in the United States: Results from the 2018 National Youth Tobacco Survey.

INTRODUCTION According to the National Youth Tobacco Survey (NYTS), youth e-cigarette use (vaping) rose between 2017-2018. Frequency of vaping and concurrent past 30-day (p30d) use of e-cigarettes and tobacco products have not been reported. METHODS We analyzed the 2018 NYTS (N=20,189) for vaping among all students (middle and high school; 6-12th grades; 9-19 years old) by frequency of vaping, exclusive vaping, p30d poly-product use (vaping and use of one or more tobacco product), and any past tobacco product use. RESULTS In 2018, 81.4% of students had not used any tobacco or vapor product in the p30d, and 86.2% had not vaped in the p30d. Among all students, of the 13.8% vaped in the p30d, just over half vaped on ≤5 days (7.0%), and roughly a quarter each vaped on 6-19 days (3.2%) and on 20+ days (3.6%). Almost three quarters of p30d vapers (9.9%) reported past or concurrent tobacco use and the remainder (3.9%) were tobacco naïve. 2.8% of students were tobacco naïve and vaped on ≤5 days; 0.7% were tobacco-naïve and vaped on 6-19 days, and 0.4% were tobacco-naïve and vaped on 20+ days. CONCLUSIONS Vaping increased among US youth in 2018 over 2017. The increases are characterized by patterns of low p30d vaping frequency and high poly-product use, and a low prevalence of vaping among more frequent but tobacco naïve vapers. IMPLICATIONS Results underscore the importance of including the full context of use patterns. The majority of vapers (60.0% - 88.9% by use frequency) were concurrent p30d or ever tobacco users. About 4% of students were tobacco naïve and vaped in the p30d, but few (0.4%) vaped regularly on 20 or more days. Reporting youth vaping data with frequency and tobacco product co-use will give public health decision-makers the best possible information to protect public health.
SIRT3, PP2A and TTP protein stability in the presence of TNF‐α on vincristine‐induced apoptosis of leukaemia cells

The contribution of vincristine (VCR)‐induced microtubule destabilization to evoke apoptosis in cancer cells remains to be resolved. Thus, we investigated the cytotoxic mechanism of VCR on U937 and HL‐60 human leukaemia cell lines. We discovered that VCR treatment resulted in the up‐regulation of TNF‐α expression and activation of the death receptor pathway, which evoked apoptosis of U937 cells. Moreover, VCR induced microtubule destabilization and mitotic arrest. VCR treatment down‐regulated SIRT3, and such down‐regulation caused mitochondrial ROS to initiate phosphorylation of p38 MAPK. p38 MAPK suppressed MID1‐modulated degradation of the protein phosphatase 2A (PP2A) catalytic subunit. The SIRT3‐ROS‐p38 MAPK‐PP2A axis inhibited tristetraprolin (TTP)‐controlled TNF‐α mRNA degradation, consequently, up‐regulating TNF‐α expression. Restoration of SIRT3 and TTP expression, or inhibition of the ROS‐p38 MAPK axis increased the survival of VCR‐treated cells and repressed TNF‐α up‐regulation. In contrast to suppression of the ROS‐p38 MAPK axis, overexpression of SIRT3 modestly inhibited the effect of VCR on microtubule destabilization and mitotic arrest in U937 cells. Apoptosis of HL‐60 cells, similarly, went through the same pathway. Collectively, our data indicate that the SIRT3‐ROS‐p38 MAPK‐PP2A‐TTP axis modulates TNF‐α expression, which triggers apoptosis of VCR‐treated U937 and HL‐60 cells. We also demonstrate that the apoptotic signalling is not affected by VCR‐elicited microtubule destabilization.
Predictive Value of Circulating SPARC-Related protein Osteonectin in Patients with Symptomatic Moderate-to-Severe Ischemic- Induced Chronic Heart Failure

Aim: To evaluate the prognostic value of circulating osteonectin for cumulative survival and hospitalization in patients with ischemic chronic heart failure (CHF). Methods: One hundred fifty four patients with ischemic symptomatic moderate-to-severe CHF were prospectively enrolled at discharge from the hospital. Observation period was up to 3 years (156 weeks). Blood samples for hematology, chemistry, and biomarker measurements were collected at baseline prior to study entry. ELISA method for measurement of circulating osteonectin (OSN) was used. Results: During a median follow-up of 2.18 years we identified 21 deaths and 106 readmissions. Medians of circulating levels of OSN in survivors patient and subjects who died were 670.96 ng/mL (95% confidence interval (CI) = 636.53- 705.35 ng/mL) and 907.84 ng/mL (95% CI = 878.02-937.60 ng/mL). Receive Operation Characteristic curve analysis has shown the best balanced cutoff point of OSN concentration for cumulative survival equal 845.15 ng/mL. A significantly divergence of Kaplan-Meier survival curves constructed for patients with high (> 845.15 ng/mL) and low (<845.15 ng/mL) concentrations of OSN was found. Circulating OSN independently predicted all-cause mortality (OR = 1.23; 95% CI = 1.10-1.36; P < 0.001), CHF-related death (OR = 1.46; 95% CI 1.22-1.80; P < 0.001), and also CHF-related readmission (OR = 1.92; 95% CI = 1.77 - 2.45; P<0.001) within 3 year of follow-up period. Conclusion: Increased circulating SPARC family member OSN associates with increased 3-year CHF-related death, all- cause mortality, and risk for readmission due to CHF.
Mechanical Irritation in Vascularized Composite Tissue Allotransplantation Triggers Localized Skin Rejection

Background. Mechanical and thermal stress has been observed to trigger skin rejection in hand-transplanted patients. This study aims to investigate this phenomenon. Methods. Syngeneic and allogeneic orthotopic hindlimb transplantations were performed using male rats (Brown Norway to Lewis). Using a specially designed device, standardized mechanical skin irritation at a force of 5 N was applied to the planta pedis of the transplanted limb for 10 days, 4 times daily for 10 minutes. Biopsies, taken on day 10 and after a 5-day observational period, were assessed for macroscopic alterations using a standardized scale, by histopathology and immunohistochemistry, and for inflammatory protein expression using Luminex technology. Results. Allogeneic animals displayed significant aggravated macroscopic skin alterations compared with naive (P < 0.0001) and syngeneic controls (P = 0.0023). Histopathology showed a trend toward higher rejection/inflammation grades in allogeneic animals compared with syngeneic controls. Minor skin alterations in syngeneic limbs recovered quickly; however, in allogeneic limbs, macroscopic skin alterations were significantly more pronounced (P < 0.0001) 5 days after irritation. Interleukin-1b and interferon-γ levels were upregulated in skin of allogeneic limbs. Conclusions. Mechanical skin irritation in vascularized composite allotransplantation can trigger localized skin inflammation consistent with rejection.
Long-term ecological research in southern Brazil grasslands: Effects of grazing exclusion and deferred grazing on plant and arthropod communities

Grazing exclusion may lead to biodiversity loss and homogenization of naturally heterogeneous and species-rich grassland ecosystems, and these effects may cascade to higher trophic levels and ecosystem properties. Although grazing exclusion has been studied elsewhere, the consequences of alleviating the disturbance regime in grassland ecosystems remain unclear. In this paper, we present results of the first five years of an experiment in native grasslands of southern Brazil. Using a randomized block experimental design, we examined the effects of three grazing treatments on plant and arthropod communities: (i) deferred grazing (i.e., intermittent grazing), (ii) grazing exclusion and (iii) a control under traditional continuous grazing, which were applied to 70 x 70 m experimental plots, in six regionally distributed blocks. We evaluated plant community responses regarding taxonomic and functional diversity (life-forms) in separate spatial components: alpha (1 x 1 m subplots), beta, and gamma (70 x 70 m plots), as well as the cascading effects on arthropod high-taxa. By estimating effect sizes (treatments vs. control) by bootstrap resampling, both deferred grazing and grazing exclusion mostly increased vegetation height, plant biomass and standing dead biomass. The effect of grazing exclusion on plant taxonomic diversity was negative. Conversely, deferred grazing increased plant taxonomic diversity, but both treatments reduced plant functional diversity. Reduced grazing pressure in both treatments promoted the break of dominance by prostrate species, followed by fast homogenization of vegetation structure towards dominance of ligneous and erect species. These changes in the plant community led to increases in high-taxa richness and abundance of vegetation-dwelling arthropod groups under both treatments, but had no detectable effects on epigeic arthropods. Our results indicate that decision-making regarding the conservation of southern Brazil grasslands should include both intensive and alleviated levels of grazing management, but not complete grazing exclusion, to maximize conservation results when considering plant and arthropod communities.
Predominance of Central Memory T Cells with High T-Cell Receptor Repertoire Diversity is Associated with Response to PD-1/PD-L1 Inhibition in Merkel Cell Carcinoma

Purpose: Merkel cell carcinoma (MCC) is an aggressive neuroendocrine skin cancer, which can be effectively controlled by immunotherapy with PD-1/PD-L1 checkpoint inhibitors. However, a significant proportion of patients are characterized by primary therapy resistance. Predictive biomarkers for response to immunotherapy are lacking. Experimental Design: We applied Bayesian inference analyses on 41 patients with MCC testing various clinical and biomolecular characteristics to predict treatment response. Further, we performed a comprehensive analysis of tumor tissue–based immunologic parameters including multiplexed immunofluorescence for T-cell activation and differentiation markers, expression of immune-related genes and T-cell receptor (TCR) repertoire analyses in 18 patients, seven objective responders, and 11 nonresponders. Results: Bayesian inference analyses demonstrated that among currently discussed biomarkers only unimpaired overall performance status and absence of immunosuppression were associated with response to therapy. However, in responders, a predominance of central memory T cells and expression of genes associated with lymphocyte attraction and activation was evident. In addition, TCR repertoire usage of tumor-infiltrating lymphocytes (TILs) demonstrated low T-cell clonality, but high TCR diversity in responding patients. In nonresponders, terminally differentiated effector T cells with a constrained TCR repertoire prevailed. Sequential analyses of tumor tissue obtained during immunotherapy revealed a more pronounced and diverse clonal expansion of TILs in responders indicating an impaired proliferative capacity among TILs of nonresponders upon checkpoint blockade. Conclusions: Our explorative study identified new tumor tissue–based molecular characteristics associated with response to anti–PD-1/PD-L1 therapy in MCC. These observations warrant further investigations in larger patient cohorts to confirm their potential value as predictive markers.
Neurovascular Perfusion Study With DynaPBV During Preoperative Balloon Test Occlusion: A Feasibility Study on Aneurysm and Tumor Lesions

Abstract To report feasibility, safety, and technical advantages of flat-detector computed tomography perfusion (FD-CTP) during balloon test occlusion (BTO) angiography studies, 10 patients patients scheduled for BTO were evaluated. Cerebral blood volume maps were extracted from FD-CTP images acquired during the test. The FD-CTP perfusion combined with BTO is feasible and safe in intracranial tumor, and aneurysm cases in which vessel sacrifice should be considered.
Assessing the Risk for Gout With Sodium–Glucose Cotransporter-2 Inhibitors in Patients With Type 2 Diabetes

Background Hyperuricemia is common in patients with type 2 diabetes mellitus and is known to cause gout. Sodium-glucose cotransporter-2 (SGLT2) inhibitors prevent glucose reabsorption and lower serum uric acid levels. Objective To compare the rate of gout between adults prescribed an SGLT2 inhibitor and those prescribed a glucagon-like peptide-1 (GLP1) receptor agonist. Design Population-based new-user cohort study. Setting A U.S. nationwide commercial insurance database from March 2013 to December 2017. Patients Persons with type 2 diabetes newly prescribed an SGLT2 inhibitor were 1:1 propensity score matched to patients newly prescribed a GLP1 agonist. Persons were excluded if they had a history of gout or had received gout-specific treatment previously. Measurements The primary outcome was a new diagnosis of gout. Cox proportional hazards regression was used to estimate hazard ratios (HRs) of the primary outcome and 95% CIs. Results The study identified 295 907 adults with type 2 diabetes mellitus who were newly prescribed an SGLT2 inhibitor or a GLP1 agonist. The gout incidence rate was lower among patients prescribed an SGLT2 inhibitor (4.9 events per 1000 person-years) than those prescribed a GLP1 agonist (7.8 events per 1000 person-years), with an HR of 0.64 (95% CI, 0.57 to 0.72) and a rate difference of -2.9 (CI, -3.6 to -2.1) per 1000 person-years. Limitation Unmeasured confounding, missing data (namely incomplete laboratory data), and low baseline risk for gout. Conclusion Adults with type 2 diabetes prescribed an SGLT2 inhibitor had a lower rate of gout than those prescribed a GLP1 agonist. Sodium-glucose cotransporter-2 inhibitors may reduce the risk for gout among adults with type 2 diabetes mellitus, although future studies are necessary to confirm this observation. Primary Funding Source Brigham and Women's Hospital.
Characteristics of intestinal microflora involved insacral nerve stimulation affecting visceral hypersensitivity.

OBJECTIVE This study was to investigate the effect of intestinal flora-intestinal-brain on the visceral sensitivity of normal rat and visceral hypersensitivity model rats, and to explore the effect of intestinal flora-intestinal-brain axis on visceral hypersensitivity in rats. METHODS Sixty SD male newborn rats were randomly divided into 4 groups according to the random number table: group A, group B, group C, and group D, with 15 rats in each group. 15 sterile SD newborn rats, numbered E group. Group A: normal model group; group B, group C, group D, group E, all used acetogen enema sensitization method to establish a visceral hypersensitivity model. Group C was given the vancomycin antibiotic before the model group and in group D sacral nerve stimulation (SNS) was given after modeling. At the end of the treatment period, the visceral sensitivity, intestinal flora expression, expression of NGF, TrKA, NF-κB, TRPV1, pTRPV1, IL-1β, IL-10, IL-22, TNF-α, 5-HT, and γ-GABA were measured in each group. RESULTS (1) The VMR values of the sterile rat model group were significantly different from those of the model group and the SNS stimulation model group (P<0.01). The VMR values of the antibiotic model group were statistically significant compared with the SNS stimulation model group (P<0.01). (2) There was no bacterial growth in the sterile rat model group. The expression levels of the four bacterial groups were significantly different between the antibiotic model group and the SNS stimulation model group (P<0.01). (3) The expression of NGF and TrKA in the SNS stimulation model was higher than that in the antibiotic model group (P<0.05). The expression of NF-κB and pTRPV1 was lower than that in the model group (P<0.05). The NGF, TrKA, NF-κB, and pTRPV1 were hardly expressed, which was significantly lower than the other groups (P<0.05). (4) There was no significant difference in the content of each index between the normal model group and the antibiotic model group (P > 0.05), IL-10 and 5-HT levels in the normal model group, the sterile rat model group, and the antibiotic model. There was no significant difference between the group and the sterile rat model group (P > 0.05). The difference was statistically significant (P<0.01). CONCLUSION The neurotransmitter produced by the intestinal flora can bind to the receptor TrkA and the translocation channel TRPV1 of intestinal tissue and CNS tissue, causing intestinal sensitivity changes.
The piRNA pathway in Drosophila ovarian germ and somatic cells

RNA silencing refers to gene silencing pathways mediated by small non-coding RNAs, including microRNAs. Piwi-interacting RNAs (piRNAs) constitute the largest class of small non-coding RNAs in animal gonads, which repress transposons to protect the germline genome from the selfish invasion of transposons. Deterioration of the system causes DNA damage, leading to severe defects in gametogenesis and infertility. Studies using Drosophila ovaries show that piRNAs originate from specific genomic loci, termed piRNA clusters, and that in piRNA biogenesis, cluster transcripts are processed into mature piRNAs via three distinct pathways: initiator or responder for ping-pong piRNAs and trailing for phased piRNAs. piRNAs then assemble with PIWI members of the Argonaute family of proteins to form piRNA-induced RNA silencing complexes (piRISCs), the core engine of the piRNA-mediated silencing pathway. Upon piRISC assembly, the PIWI member, Piwi, is translocated to the nucleus and represses transposons co-transcriptionally by inducing local heterochromatin formation at target transposon loci.
Single-Atom Enzyme Functionalized Solution-Gated Graphene Transistor for Real-Time Detection of Mercury Ion.

Mercury ion (Hg2+), a bioaccumulating and toxic heavy metal, can cause severe damages to the environment and human health. Therefore, development of high-performance Hg2+ sensors is highly desirable. Herein, we construct a uniform dodecahedral shaped N-doped carbon decorated by single Fe sites enzyme (Fe-N-C SAE), which exhibits a good performance for Hg2+ detection. The N atom on Fe-N-C SAE can specifically recognize Hg2+ through the chelation between Hg2+ and N atom, while the catalytic site on the single-atom enzyme acts as a signal amplifier. The Fe-N-C SAE functionalized solution-gated graphene transistor exhibits a dramatic improvement in the selectivity and sensitivity of the devices. The sensor can rapidly detect Hg2+ down to 1 nM within 2 seconds. Besides, a relatively good repeatability and reproducibility for the detection of Hg2+ has also been found in our sensor platform. Our findings expand the application of single atom catalyst in the field of food safety and environmental monitoring.
The Future of Parkinson Care and Research is Here.

The field of Parkinson’s disease research is increasingly dynamic. These are promising times for affected patients and their families, with improved healthcare services being in practice already, and with new potentially disease-modifying treatments on the horizon. It is also a gratifying time for clinicians, who now can provide more hope when conveying the diagnosis to patients, and who can offer them new evidence-based treatments. And these are certainly also exciting times for scientists, who can capitalise on new technologies which are providing fast-rising insights in fundamental disease mechanisms, who can develop innovative interventions tailored to these novel concepts, and build a robust evidence base for them. This new, rich and versatile issue of the Journal of Parkinson’s Disease is a good illustration of these many promising developments in the field, and we hope you will enjoy reading as much we did. Meanwhile, the journal itself is taking a big step forward. One important new development for this coming year is the arrival of Bas Bloem as the new co-Editor-in-Chief, who will replace Bill Langston