Miracle-dz/UniFAR-FTUs huggingface dataset:
The plant–pathogen haustorial interface at a glance

ABSTRACT Many filamentous pathogens invade plant cells through specialized hyphae called haustoria. These infection structures are enveloped by a newly synthesized plant-derived membrane called the extrahaustorial membrane (EHM). This specialized membrane is the ultimate interface between the plant and pathogen, and is key to the success or failure of infection. Strikingly, the EHM is reminiscent of host-derived membrane interfaces that engulf intracellular metazoan parasites. These perimicrobial interfaces are critical sites where pathogens facilitate nutrient uptake and deploy virulence factors to disarm cellular defenses mounted by their hosts. Although the mechanisms underlying the biogenesis and functions of these host–microbe interfaces are poorly understood, recent studies have provided new insights into the cellular and molecular mechanisms involved. In this Cell Science at a Glance and the accompanying poster, we summarize these recent advances with a specific focus on the haustorial interfaces associated with filamentous plant pathogens. We highlight the progress in the field that fundamentally underpin this research topic. Furthermore, we relate our knowledge of plant–filamentous pathogen interfaces to those generated by other plant-associated organisms. Finally, we compare the similarities between host–pathogen interfaces in plants and animals, and emphasize the key questions in this research area. Summary: A discussion of the distinct host cell transport routes that underpin the biogenesis and function of plant–pathogen interfaces.
ICTV Virus Taxonomy Profile: Closteroviridae

Viruses in the family Closteroviridae have a mono-, bi- or tripartite positive-sense RNA genome of 13–19 kb, and non-enveloped, filamentous particles 650–2200 nm long and 12 nm in diameter. They infect plants, mainly dicots, many of which are fruit crops. This is a summary of the ICTV Report on the family Closteroviridae, which is available at ictv.global/report/closteroviridae.
Canonical Correlation between Behavioral-Psychological Variables and Predictors of Coronary Artery Disease Prognosis

Metabolic syndrome (MetS) and severity of coronary artery disease (CAD) are considered predictors of CAD prognosis. Unhealthy lifestyles and type-D personality are associated with MetS and are potential causes of primary and secondary CAD. In this cross-sectional descriptive study, we aimed to investigate the relationship between behavioral-psychological variables and predictors of CAD prognosis. The behavioral-psychological variable set contained six lifestyle categories and two type-D personality categories. Descriptive analyses, t-tests, analysis of variance, Pearson’s correlation, and canonical correlation were used. The behavioral-psychological variable set was related to the predictor set for CAD prognosis, with a significant canonical variate of 0.67 (45% overlapping variance). Significant pairs of canonical variates indicated that poor physical activity and weight control (−0.77), poor dietary habits (−0.78), alcohol consumption and cigarette smoking (−0.37), lack of sleep and rest (−0.40), stress (−0.64) in the lifestyle set, higher negative affectivity (0.52), and social inhibition (0.71) in the type-D personality set were associated with a high MetS score (0.59) and severity of CAD (0.91). A combination of behavioral and psychological variables was found to be important in predicting the prognosis of CAD; therefore, interventions aimed at preventing combinations of these variables may be effective in improving CAD prognosis.
Materials as Machines

Machines are systems that harness input power to extend or advance function. Fundamentally, machines are based on the integration of materials with mechanisms to accomplish tasks—such as generating motion or lifting an object. An emerging research paradigm is the design, synthesis, and integration of responsive materials within or as machines. Herein, a particular focus is the integration of responsive materials to enable robotic (machine) functions such as gripping, lifting, or motility (walking, crawling, swimming, and flying). Key functional considerations of responsive materials in machine implementations are response time, cyclability (frequency and ruggedness), sizing, payload capacity, amenability to mechanical programming, performance in extreme environments, and autonomy. This review summarizes the material transformation mechanisms, mechanical design, and robotic integration of responsive materials including shape memory alloys (SMAs), piezoelectrics, dielectric elastomer actuators (DEAs), ionic electroactive polymers (IEAPs), pneumatics and hydraulics systems, shape memory polymers (SMPs), hydrogels, and liquid crystalline elastomers (LCEs) and networks (LCNs). Structural and geometrical fabrication of these materials as wires, coils, films, tubes, cones, unimorphs, bimorphs, and printed elements enables differentiated mechanical responses and consistently enables and extends functional use.
The Use of the Nicotine Metabolite Ratio as a Biomarker to Personalize Smoking Cessation Treatment: Current Evidence and Future Directions

The nicotine metabolite ratio (NMR), a genetically informed biomarker of rate of nicotine metabolism, has been validated as a tool to select the optimal treatment for individual smokers, thereby improving treatment outcomes. This review summarizes the evidence supporting the development of the NMR as a biomarker of individual differences in nicotine metabolism, the relationship between the NMR and smoking behavior, the clinical utility of using the NMR to personalize treatments for smoking cessation, and the potential mechanisms that underlie the relationship between NMR and smoking cessation. We conclude with a call for additional research necessary to determine the ultimate benefits of using the NMR to personalize treatments for smoking cessation. These future directions include measurement and other methodologic considerations, disseminating this approach to at-risk subpopulations, expanding the NMR to evaluate its efficacy in predicting treatment responses to e-cigarettes and other noncigarette forms of nicotine, and implementation science including cost-effectiveness analyses. See all articles in this Special Collection Honoring Paul F. Engstrom, MD, Champion of Cancer Prevention
Inactivation and Damage of Histamine-Forming Bacteria by Treatment with High Hydrostatic Pressure

The inactivation and damage of histamine-forming bacteria (HFB), Enterobacter aerogenes and Staphylococcus capitis, in a 0.1 M potassium phosphate buffer (pH 6.8) and marlin meat slurry by high hydrostatic pressure (HHP) treatments were studied using viability measurement and scanning electron microscopy (SEM). HHP treatments showed first order destruction kinetics to E. aerogenes and S. capitis during the pressure holding period. HFB in marlin meat slurry had higher D values and were more resistant to HHP treatments than in phosphate buffer. In phosphate buffer, E. aerogenes had higher D values than S. capitis at >380 MPa of pressure, whereas the reverse trend was noticed at lower pressures (<380 MPa). In marlin meat slurry, S. capitis had a higher D value than E. aerogenes at the same treatment pressure, indicating that S. capitis was more resistant to HHP treatment. To our knowledge, this is the first report to demonstrate that HHP can be used to inactivate HFB, E. aerogenes, and S. capitis, by causing disruption to bacterial cell membrane and cell wall as demonstrated by SEM micrographs.
Why Does the Halophyte Mesembryanthemum crystallinum Better Tolerate Ni Toxicity than Brassica juncea: Implication of Antioxidant Defense Systems

The implication of enzymatic and non-enzymatic antioxidative systems in response to Ni was evaluated in the halophyte Mesembryanthemum crystallinum in comparison with the metal tolerant glycophyte species Brassica juncea. Seedlings of both species were hydroponically subjected during 21 days to 0, 25, 50, and 100 µM NiCl2. Growth parameters showed that the halophyte M. crystallinum was more tolerant to Ni than B. juncea. Malondialdehyde (MDA) content increased to a higher extent in B. juncea than in M. crystallinum. Antioxidant enzymesactivities were differently affected by Ni in both species. Nickel increased shoot superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities in B. juncea, whereas these activities were reduced in M. crystallinum when exposed to metal stress. The root SOD, APX and guaiacol peroxidase (GPX) activities increased upon Ni treatments for both species. The content of non-enzymatic antioxidative molecules such as glutathione, non-protein thiols and proline increased in Ni-treated plants, except for GSH content in the shoot of B. juncea. Based on the oxidative balance, our findings confirm the higher tolerance of the halophyte M. crystallinum to Ni-induced oxidative stress comparatively to B. juncea. We suggest that M. crystallinum is able to overcome the produced ROS using the non-enzymatic system, while Ni-induced oxidative stress was more acute in B. juncea, leading this species to mainly use the enzymatic system to protect against reactive oxygen species.
Early Childhood Antibiotic Treatment for Otitis Media and Other Respiratory Tract Infections Is Associated With Risk of Type 1 Diabetes: A Nationwide Register-Based Study With Sibling Analysis

OBJECTIVE The effect of early-life antibiotic treatment on the risk of type 1 diabetes is debated. This study assessed this question, applying a register-based design in children up to age 10 years including a large sibling-control analysis. RESEARCH DESIGN AND METHODS All singleton children (n = 797,318) born in Sweden between 1 July 2005 and 30 September 2013 were included and monitored to 31 December 2014. Cox proportional hazards models, adjusted for parental and perinatal characteristics, were applied, and stratified models were used to account for unmeasured confounders shared by siblings. RESULTS Type 1 diabetes developed in 1,297 children during the follow-up (median 4.0 years [range 0–8.3]). Prescribed antibiotics in the 1st year of life (23.8%) were associated with an increased risk of type 1 diabetes (adjusted hazard ratio [HR] 1.19 [95% CI 1.05–1.36]), with larger effect estimates among children delivered by cesarean section (P for interaction = 0.016). The association was driven by exposure to antibiotics primarily used for acute otitis media and respiratory tract infections. Further, we found an association of antibiotic prescriptions in pregnancy (22.5%) with type 1 diabetes (adjusted HR 1.15 [95% CI 1.00–1.32]). In general, sibling analysis supported these results, albeit often with statistically nonsignificant associations. CONCLUSIONS Dispensed prescription of antibiotics, mainly for acute otitis media and respiratory tract infections, in the 1st year of life is associated with an increased risk of type 1 diabetes before age 10 years, most prominently in children delivered by cesarean section.
Completing Linnaeus’s inventory of the Swedish insect fauna: Only 5,000 species left?

Despite more than 250 years of taxonomic research, we still have only a vague idea about the true size and composition of the faunas and floras of the planet [1–4]. Many biodiversity inventories provide limited insight because they focus on a small taxonomic subsample or a tiny geographic area [5, 6]. Here, we report on the size and composition of the Swedish insect fauna, representing roughly half the macroscopic diversity of one of the largest European countries, based on more than a decade of data from the Swedish Taxonomy Initiative and a massive inventory of the country’s insect fauna [7, 8]. The fauna is considered one of the best known in the world, but the inventory has nevertheless revealed a surprising amount of hidden diversity: more than 3,000 new species (301 new to science) have been documented so far. We show that three independent extrapolation methods converge on similar estimates of the true size and composition of the true fauna, suggesting that it comprises around 33,000 species. Of those, 8,600 (26%) were unknown at the start of the inventory and 5,500 (17%) still await discovery. Most of the new species belong to Hymenoptera and Diptera groups that are decomposers or parasitoids. Thus, current knowledge of the Swedish insect fauna is strongly biased taxonomically and ecologically, and we argue that this is likely true for most insect faunas. Addressing these biases is critical in understanding insect biomes and the ecosystem services they provide.
Distillation of an End-to-End Oracle for Face Verification and Recognition Sensors †

Face recognition functions are today exploited through biometric sensors in many applications, from extended security systems to inclusion devices; deep neural network methods are reaching in this field stunning performances. The main limitation of the deep learning approach is an inconvenient relation between the accuracy of the results and the needed computing power. When a personal device is employed, in particular, many algorithms require a cloud computing approach to achieve the expected performances; other algorithms adopt models that are simple by design. A third viable option consists of model (oracle) distillation. This is the most intriguing among the compression techniques since it permits to devise of the minimal structure that will enforce the same I/O relation as the original model. In this paper, a distillation technique is applied to a complex model, enabling the introduction of fast state-of-the-art recognition capabilities on a low-end hardware face recognition sensor module. Two distilled models are presented in this contribution: the former can be directly used in place of the original oracle, while the latter incarnates better the end-to-end approach, removing the need for a separate alignment procedure. The presented biometric systems are examined on the two problems of face verification and face recognition in an open set by using well-agreed training/testing methodologies and datasets.
Is bimanual interference affected in the case of a central proprioceptive loss? New insight from a left-brain-damaged single-case study.

OBJECTIVE It was suggested that the bimanual coupling effect might be linked to motor intentionality and planning, which are the top-down components of motor execution. However, previous results in pathological and healthy individuals have also underlined the pivotal role of bottom-up sensorimotor information. METHOD In this single-case study, the Circles-Lines Coupling Task was administered to a left-parietal-brain-damaged individual. The cerebral lesion caused a central proprioceptive loss, relative to the impaired right hand, when out of the visual control. For the 1st time in literature, we sought to investigate whether the movement of the unaffected hand induced an efficient coupling effect on the movement of the affected one. The bimanual task was performed in the presence and absence of visual input. The patient's performance was compared with that of healthy controls. RESULTS We observed the traditional bimanual coupling effect in healthy controls. Moreover, we also replicated the effect when they performed the task blindfolded. In the case of the patient, both hands showed the typical ovalization of the line trajectory when the task was performed in visual modality. It is interesting that when the patient performed the task blindfolded, the trajectories of the impaired right hand seemed to be not influenced by the concomitant circular movement of the spared left hand. CONCLUSIONS The movement of the unaffected hand induced a bimanual coupling effect on the movement of the affected one only when the visual input was available. In absence of a visual feedback, the aberrant proprioceptive information might preclude the emerging of bimanual coupling, even in the case of a preserved motor intentionality and planning. (PsycINFO Database Record (c) 2020 APA, all rights reserved).
Mulher trabalhadora e fatores que interferem na amamentação: revisão integrativa

Pesquisas mostram que as mulheres consideram o trabalho e continuação do aleitamento materno como práticas incompatíveis. Este trabalho apresenta como objetivo analisar as evidências científicas acerca das barreiras e facilitadores no aleitamento materno após retorno da mulher ao trabalho. A busca bibliográfica deu-se nas bases de dados SCOPUS, LILACS, PUBMED e CINAHL. A amostra foi composta de 20 artigos publicados no período de 2001 a 2011. Os artigos foram agrupados e os resultados divididos em cinco categorias temáticas: experiências maternas frente à amamentação; fatores relacionados à duração da amamentação; trabalho e amamentação; políticas de trabalho e amamentação; programas de amamentação; mulher trabalhadora e fatores biológicos. Este estudo é fundamental para profissionais da saúde de diversas áreas, uma vez que se configura como ferramenta primordial para identificação de estratégias específicas para a melhoria da assistência à mulher trabalhadora.
Effects of Scanning Strategy and Scanner Type on the Accuracy of Intraoral Scans: A New Approach for Assessing the Accuracy of Scanned Data.

PURPOSE This study aimed to determine the most reliable scanning strategy and scanner type, using a new protocol for assessing the accuracy (trueness and precision) of intraoral scan data. MATERIALS AND METHODS Five different maxillary and mandibular typodont pairs (n = 10) and 2 intraoral scanners were used for the study. A reference scan for each arch was obtained with an industrial scanner. Scanning strategies were classified into 2 continuous methods-continuous scan in horizontal direction (CH group) and continuous scan with vertical rotation in anterior region (CV group)-and 1 segmental method (S group). In the CH group, the scanner head was maintained mostly in a horizontal position. In the CV group, the scanners were rotated 180° around the anterior tooth region to allow smooth scanning through the area. The intraoral scan data were individually superimposed over their corresponding reference scan data. Raw data of the distances between paired surface points were extracted from the superimposed pairs of datasets, with (original distance values) or without consideration (absolute distance values) of the value signs. Trueness values were calculated using absolute distance values, while precision values were obtained from original distance values. Data were analyzed with a 2-way repeated-measures analysis of variance using α = 0.05 as the level of significance. RESULTS The CV group produced significantly inferior outcomes compared to the CH and S groups in terms of trueness (p < 0.001, F = 24.67), whereas no significant differences were observed among the 3 scanning strategies with respect to precision (p = 0.451, F = 0.83). Scanner type did not produce significant differences in terms of either trueness (p = 0.058, F = 4.72) or precision (p = 0.742, F = 0.12). CONCLUSIONS The segmental approach for scanning the region of interest first and continuous scanning with the scanner head held mostly in a horizontal position are both acceptable as full-arch scanning strategies. However, vertical rotation of intraoral scanners should be minimized. This article is protected by copyright. All rights reserved.
Avelumab (MSB0010718C; anti-PD-L1) vs platinum-based doublet as first-line treatment for metastatic or recurrent PD-L1-positive non-small-cell lung cancer: The phase 3 JAVELIN Lung 100 trial.

TPS9105Background: Programmed death-1 receptor ligand (PD-L1) is a key therapeutic target in the reactivation of the immune response against multiple cancers. Avelumab* is a fully human anti-PD-L1 IgG1 antibody that has shown promising efficacy and an acceptable safety profile in multiple tumor types, including non-small-cell lung cancer (NSCLC). This open-label phase 3 trial (NCT02576574) compares single-agent avelumab vs platinum-based doublet chemotherapy as first-line treatment for patients (pts) with PD-L1-positive (+) recurrent or stage IV NSCLC negative for activating EGFR mutation and ALK translocation. Methods: The primary objective of this global, multicenter, randomized trial is to demonstrate superiority of avelumab in improving progression-free survival (PFS) according to RECIST 1.1 and as assessed by an Independent Review Committee (IRC) vs platinum-based doublet chemotherapy. Approximately 420 pts with PD-L1+ tumors, as determined by companion diagnostic assay, will be randomized. Eligibili.
Study on Quantitative Analysis of Carbon and Nitrogen in Stoichiometric θ-Fe3C and γ′-Fe4N by Atom Probe Tomography

Abstract The quantitative analysis performance of carbon and nitrogen was investigated using stoichiometric θ-Fe3C (25 at% C) and γ′-Fe4N (~20 at% N) precipitates in pulsed voltage and pulsed laser atom probes. The dependencies of specimen temperature, pulse fraction, and laser pulse energy on the apparent concentrations of carbon and nitrogen were measured. Good coincidence with 25 at% carbon concentration in θ-Fe3C was obtained for the pulsed voltage atom probe by considering the mean number of carbon atoms per ion at 24 Da and the detection loss of iron, while better coincidence was obtained for the pulsed laser atom probe by considering only the mean number of carbon at 24 Da. On the other hand, a lack of nitrogen concentration in γ′-Fe4N was observed for the two atom probes. In particular, the pulsed laser atom probe showed a significant lack of nitrogen concentration. This implies that a large amount of 14N2+ was obscured by the main iron peak of 56Fe2+ at 28 Da in the mass-to-charge spectrum. Regarding preferential evaporation or retention, carbon in θ-Fe3C exhibited little of either, but nitrogen in γ′-Fe4N exhibited definite preferential retention. This result can be explained by the large difference in ionization energy between carbon and nitrogen.
Cannabis Microbiome and the Role of Endophytes in Modulating the Production of Secondary Metabolites: An Overview

Plants, including cannabis (Cannabis sativa subsp. sativa), host distinct beneficial microbial communities on and inside their tissues and organs, including seeds. They contribute to plant growth, facilitating mineral nutrient uptake, inducing defence resistance against pathogens, and modulating the production of plant secondary metabolites. Understanding the microbial partnerships with cannabis has the potential to affect the agricultural practices by improving plant fitness and the yield of cannabinoids. Little is known about this beneficial cannabis-microbe partnership, and the complex relationship between the endogenous microbes associated with various tissues of the plant, and the role that cannabis may play in supporting or enhancing them. This review will consider cannabis microbiota studies and the effects of endophytes on the elicitation of secondary metabolite production in cannabis plants. The review aims to shed light on the importance of the cannabis microbiome and how cannabinoid compound concentrations can be stimulated through symbiotic and/or mutualistic relationships with endophytes.