Miracle-dz/UniFAR-FTUs huggingface dataset:
Implementation Lessons from a Multifaceted National Newborn Program in Solomon Islands: A Mixed-Methods Study.

Multifaceted interventions are important in improving neonatal quality of care and health outcomes. This study describes the implementation of an intervention to improve the quality of newborn care in the Solomon Islands, a small island developing state and lower middle-income country in the Western Pacific. Inputs included training, equipment provision, and healthcare system organizational changes. For evaluation, we used a mixed-methods design, using quantitative (audits of health facility equipment, structure, and organization) and qualitative (semi-structured interviews and focused group discussions with healthcare workers) methods. Participants highlighted the practical, interactive, coaching style of training and its short duration as positive features in establishing skills. Training had indirect impacts through improving culture of the workplace, and the evaluation provided a valuable opportunity for reflection of the implementation process for healthcare workers. Facility limitations from equipment deficits and poor condition of clinical areas had implications by limiting the provision of quality care, as well as contributing to healthcare workers feeling undervalued. Resuscitation of a nonbreathing baby was a stressful experience for many health workers, compounded by geographic isolation and feeling unsupported. Our findings highlight the importance of training methodology, impact from structural limitations, and experience of resuscitation for the healthcare worker. Attention to these factors may help the design and implementation of newborn care programs in similar contexts.
Interactive Abstract Painting by Augmented Reality: Scenarios and Architecture Solutions

This article overviews services and platforms applied for application of technology of augmented reality (AR) to improve human-machine and paint-ing-human interaction during observing abstract paintings. Besides, scenarios of AR application for fluid abstract paintings are systemized and discussed. The service based on project Artivive implementing AR for paintings is analysed. The approaches and architecture patterns for service, based on different scenarios are discussed. The developed architecture designs can be used to make the galleries of abstract paintings more interactive and engage more visitors. The suggested architecture solution (the project AR for Smart Abstract Art, ARSmArt) for the selected scenario “AR by Individual Visitor” is presented in case study. The ARSmArt architecture is compared to the existing Artivive based service. Future steps of research and development are described
Tightly Coupled GNSS/INS Integration with Robust Sequential Kalman Filter for Accurate Vehicular Navigation

With the development of multi-constellation multi-frequency Global Navigation Satellite Systems (GNSS), more and more observations are available for tightly coupled GNSS/Inertial Navigation System (INS) integration. Concerning the accuracy, robustness, and computational burden issues in the integration, we proposed a robust and computationally efficient implementation. The new tight integration model uses pseudorange, Doppler and carrier phase simultaneously, to achieve the maximum possible navigation accuracy for a single receiver. The resultant high-dimensional observation vector is then processed by a sequential Kalman Filter (KF) to improve the computational efficiency in the measurement update step. Based on the innovation of the sequential KF, a robust estimation method with Gaussian test is further devised to detect and adapt the faults in individual GNSS channels. Two field vehicular tests are conducted to evaluate the performance improvements of the proposed method, compared with loose coupling and conventional tight coupling. Test results in favorable environments indicate that the proposed method can significantly improve the velocity and attitude accuracy by 69.42% and 47.16% over loose coupling and by 64.75% and 30.88% over conventional tight coupling, respectively. Moreover, the computational efficiency is also improved by about 53.09% for the proposed method, compared with batch KF processing. In GNSS challenging environments, the proposed method also shows superiority in terms of velocity and attitude accuracy, and better bridging capability during the GNSS partial or complete outages. These results demonstrate that the proposed method is able to provide a more robust and accurate solution in real-time vehicular navigation.
Selecting Training-Load Measures to Explain Variability in Football Training Games

The purpose of this study was to investigate the structure of interrelationships among external (eT) and internal (iT) training intensity metrics and how these vary depending on game format in soccer. The variables were collected from 16 semi-professional players in seven types of small, medium, large-sided, and simulated games (SG). The eT variables were (per min): peak velocity (Vmax), total distance (DTmin), distance covered at velocities less than 60% (D < 60%min), between 60 and 80% (D > 60%min), and more than 80% (D > 80%min) of the maximal velocity, player load (PLmin), and distance covered accelerating at more than 2 m⋅s-2 (Daccmin) and decelerating at less than −2 m⋅s-2 (Ddecmin). The iT variables were: Edwards arbitrary units (EDWmin) and time spent at more than 80% of the maximal heart rate (T > 80% HRmin). All game formats were represented by three principal components (PC), explaining from 66.9 to 76.0% of the variance. The structure of the interrelationships among variables involved similar distributions in the PCs that are related to energetic production systems, such as the strength/neuromuscular dimension (PLmin and/or Daccmin and Ddecmin, complemented by DTmin and D < 60%min), the endurance/cardiovascular dimension (EDWmin), and the velocity/locomotion dimension (Vmax, D > 60%min, or D > 80%min). A particular combination of external and internal intensity measures is required to describe the training load of game formats.
Multiregional origins of the domesticated tetraploid wheats

We used genotyping-by-sequencing (GBS) to investigate the evolutionary history of domesticated tetraploid wheats. With a panel of 189 wild and domesticated wheats, we identified 1,172,469 single nucleotide polymorphisms (SNPs) with a read depth ≥3. Principal component analyses (PCAs) separated the Triticum turgidum and Triticum timopheevii accessions, as well as wild T. turgidum from the domesticated emmers and the naked wheats, showing that SNP typing by GBS is capable of providing robust information on the genetic relationships between wheat species and subspecies. The PCAs and a neighbour-joining analysis suggested that domesticated tetraploid wheats have closest affinity with wild emmers from the northern Fertile Crescent, consistent with the results of previous genetic studies on the origins of domesticated wheat. However, a more detailed examination of admixture and allele sharing between domesticates and different wild populations, along with genome-wide association studies (GWAS), showed that the domesticated tetraploid wheats have also received a substantial genetic input from wild emmers from the southern Levant. Taking account of archaeological evidence that tetraploid wheats were first cultivated in the southern Levant, we suggest that a pre-domesticated crop spread from this region to southeast Turkey and became mixed with a wild emmer population from the northern Fertile Crescent. Fixation of the domestication traits in this mixed population would account for the allele sharing and GWAS results that we report. We also propose that feralization of the component of the pre-domesticated population that did not acquire domestication traits has resulted in the modern wild population from southeast Turkey displaying features of both the domesticates and wild emmer from the southern Levant, and hence appearing to be the sole progenitor of domesticated tetraploids when the phylogenetic relationships are studied by methods that assume a treelike pattern of evolution.
Computed tomographic quantification of periaortic adipose tissue volume as a correlate of cardiovascular disease

The accumulation of adipose tissue has long been thought of as a risk factor for cardiovascular disease (CVD) [1]. However, the dynamic effects in metabolic homeostasis and pathology are still being discovered [2]. The complexity of factors associated with obesity including adipokines associated with various adipose tissue depots is frequently overlooked [3]. Intuitively, one of these depots, perivascular adipose tissue (PVAT), is likely contributing to the development and/or progression of CVD given its proximity to arterial vasculature. A subset of PVAT that surrounds the coronary arteries, epicardial fat, has been well studied in the development of coronary artery disease [4]. Another subset of PVAT, periaortic adipose tissue has also been implicated in CVD, however, previous studies have limited their investigation to a specific population, unique depots, or a single CVD [5–7]. Herein, the current study describes the quantification of periaortic adipose tissue volume in both the thoracic and abdominal regions in a unique population and correlates this value to specific measures of CVD. Once the study protocol and procedures were approved by the University of Cincinnati Institutional Review Board (IRB#2013-8286), An electronic medical record was queried for patients who obtained non-contrasted computed tomography (CT) scans of their chest and/or abdomen over a 2-year period. These deidentified records were reviewed to verify subjects met inclusion/exclusion criteria which were broad, allowing for a diverse patient population. Inclusion criteria included 18 years of age or older and non-contrasted CT scan of chest and/or abdomen. Exclusion criteria where limited to variables that would compromise the measurement of the periaortic tissue volume including previous aortic surgery, gross anatomical anomalies (including trauma) or the use of contrast as the increased attenuation within the lumen would alter the radiodensity of the surrounding tissue. After appropriate subjects were identified, abdominal and thoracic periaortic adipose tissue volume was quantified in a similar manner as previously conducted [5]. The segment of thoracic aorta that was measured started at the level of the pulmonary artery bifurcation and extended 70 mm inferiorly. The abdominal aortic segment was measured starting at the level of the aortic bifurcation and extended superiorly 40 mm. Adipose tissue was selectively gated using a window width of –195 to –45 Hounsfield units (HU) with a center of –120 HU [5]. The region of interest was encircled with a diameter being 10 mm larger than the anteroposterior aortic diameter and then adipose tissue was selectively gated [6]. The degree of aortic calcification was determined by the volume of hyperattenuation with a minimum of three connected pixels with attenuation over 130 HU [5]. CLINICAL CARDIOLOGY
Cell Biology International

Expansion above a certain threshold in the polyglutamine (polyQ) tract of ataxin‐3 is the main cause of neurodegeneration in Machado–Joseph disease. Ataxin‐3 contains an N‐terminal catalytic domain, called Josephin domain, and a highly aggregation‐prone C‐terminal domain containing the polyQ tract. Recent work has shown that protein aggregation inhibits clathrin‐mediated endocytosis (CME). However, the effects of polyQ expansion in ataxin‐3 on CME have not been investigated. We hypothesize that the expansion of the polyQ tract in ataxin‐3 could impact CME. Here, we report that both the wild‐type and the expanded ataxin‐3 reduce transferrin internalization and expanded ataxin‐3 impacts dynamics of clathrin‐coated pits (CCPs) by reducing CCP nucleation and increasing short‐lived abortive CCPs. Since endocytosis plays a central role in regulating receptor uptake and cargo release, our work highlights a potential mechanism linking protein aggregation to cellular dysregulation.
Dynamics Insights Into the Gain of Flexibility by Helix-12 in ESR1 as a Mechanism of Resistance to Drugs in Breast Cancer Cell Lines

Incidents of breast cancer (BC) are on the rise on a daily basis and have proven to be the most prevelant cause of death for women in both developed and developing countries. Among total BC cases diagnosed after menopause, 70% of cases are Estrogen Receptor (ER) positive (ER-positive or ER+). Mutations in the LBD (ligand-binding domain) of the ER have recently been reported to be the major cause of resistance to potent antagonists. In this study, the experimentally reported mutations K303R, E380Q, V392I, S463P, V524E, P535H, P536H, Y537C, Y537N, Y537S, and D538G were analyzed, and the most significant mutations were shortlisted based on multiple analyses. Initial analyses, such as mCSM stability, occluded depth analysis, mCSM-binding affinity, and FoldX energy changes shortlisted only six mutations as being highly resistant. Finally, simulations of force field-based molecular dynamics (MD on wild type (WT) ERα) on six mERα variants (E380Q, S463P, Y537S, Y537C, Y537N, and D538G) were carried out to justify mechanism of the resistance. It was observed that these mutations increased the flexibility of the H12. A bonding analysis suggested that previously reported important residue His524 lost bonding upon mutation. Other parameters, such as PCA (principal component analysis), DCCM (dynamics cross-correlation), and FEL (free energy landscape), verified that the shortlisted mutations affect the H12 helix, which opens up the co-activator binding conformation. These results provide deep insight into the mechanism of relative resistance posed to fulvestrant due to mutations in breast cancer. This study will facilitate further understanding of the important aspects of designing specific and more effective drugs.
USAGE OF STEEL SLAG AS A CONSTRUCTION MATERIAL AND IN ENVIRONMENTAL APPLICATIONS

Steel slag is a by-product in the production of steel. An increase of its production results in more landfills. It is mostly used as a building material in the construction of roads, where it substitutes natural materials. The use of steel slag in road construction has multiple benefits; both by solving the challenges of waste disposal and preserving environmental resources by moderating the consumption of non-renewable and natural aggregates. Apart from being used as a building material, steel slag is also used in numerous environmental applications, such as soil and water remediation and CO2 sequestration. However, a multidisciplinary environmental impact study of steel slag, particularly regarding both the aquatic and terrestrial organisms, is lacking. Steel slag contains traces of potentially harmful elements that endanger these living organisms, including heavy metals released into the environment. A high pH value can also significantly influence their leaching properties, thereby affecting the organisms in the water and soil. Given the widespread utility of steel slag, it is recommended to make a thorough risk assessment associated with the various environmental applications of this material.
Afterdischarges of Spinal Interneurons Following a Brief High-Frequency Stimulation of Ia Afferents in the Cat

Spinal motoneurons exhibit sustained afterdischarges and plateau potentials following a brief high-frequency stimulation of Ia afferents. Also, there is evidence that spinal cord interneurons exhibit plateau potentials. However, to our knowledge, there are no reports about the possible afterdischarge behavior of lumbar spinal interneurons activated by Ia afferents. Given that there are spinal interneurons receiving monosynaptic inputs from Ia afferents, these cells could then be activated in parallel to motoneurons after repetitive muscle stretch. We explored this possibility in cats with a precollicular-postmammillary decerebration. We found that a brief high-frequency stimulation of Ia afferents produces afterdischarges that are highly correlated to a DC slow potential recorded at the cord dorsum. We conclude that in the cat spinal cord, not only the motoneurons but also the interneurons from the superficial and deep dorsal horn produce sustained afterdischarges, thus highlighting the importance of interneurons in the spinal neuronal circuitry. The significance of our finding is that it opens the possibility that the spinal cord interneurons activated by Ia afferents could also exhibit bistability, a relevant phenomenon well-characterized in the motoneurons.
Efficacy of Self-Management Smartphone-Based Apps for Post-traumatic Stress Disorder Symptoms: A Systematic Review and Meta-Analysis

Post-traumatic stress disorder (PTSD) symptoms are prevalent in both civilian and military service members. As the number of smartphone-based applications (apps) grows rapidly in health care, apps are also increasingly used to help individuals with subthreshold PTSD or full PTSD. Yet, if the apps are self-managed, the feasibility and efficacy of such interventions are still rather unclear in these two populations with PTSD symptoms. Hence, the present meta-analysis set out to evaluate the effect of self-management smartphone-based apps on PTSD and depressive symptoms in populations with subthreshold PTSD or full PTSD. Studies were included if they conducted randomized controlled trials or pre-post comparisons. Six studies (n = 2 randomized controlled trials) were identified for meta-analysis. In pre-post comparisons, N = 209 participants were included in the analyses. In randomized controlled trials, N = 87 participants received smartphone-based self-management interventions and N = 82 participants were in waitlist control conditions. Meta-analysis for pre-post comparisons concluded an effect of g = 0.55 (p < 0.001) regarding the overall reduction in PTSD symptoms (n = 6) and g = 0.45 (p < 0.001) for reduction in depressive symptoms (n = 5). Yet, in randomized controlled trials, no significant difference was found between app-based treatment and waitlist control groups (g = 0.09, p = 0.574). The duration of the interventions did not significantly influence the results. Overall, despite positive pre-post effects, current results indicate that smartphone-apps for PTSD patients are not significantly more effective than waitlist control conditions. Nevertheless, a combined smartphone and standard therapy approach may be a fruitful field for future research.
Comparison of the Antennal Sensilla and Compound Eye Sensilla in Four Drosophila (Diptera: Drosophilidae) Species

Abstract Antennal sensilla and compound eyes of fruit flies, Drosophila melanogaster (Meigen), Drosophila suzukii (Matsumura), Drosophila immigrans (Sturtevant), and Drosophila hydei (Sturtevant) (all Diptera: Drosophilidae), were observed with stereoscopic microscopy and scanning electron microscopy. The results showed that the antenna consists of 3 segments: a proximal scape, a pedicel, and a flagellum composed of a funiculus and a dendritic arista. Six morphologically distinct types of sensilla were observed on the antenna: chaetica, microtrichia, trichoid (Tr I, Tr II), basiconic, clavate, and coeloconic (Co I, Co II). Basiconic sensilla were significantly shorter than other sensilla in the funiculus of D. melanogaster. The number of clavate sensillae was less than other sensilla in the funiculus of D. melanogaster and D. immigrans, but was greater in D. suzukii and D. hydei. Moreover, coeloconic sensilla were absent in D. suzukii and D. hydei. The length and abundance of the chaetica sensilla on the compound eyes were different significantly among the 4 species. Drosophila hydei had chaotic sensilla with the greatest length and abundance; they were lowest for D. suzukii. Based on the previous literature, the possible functions of these sensilla are discussed. We inferred that fruit flies may regulate their behaviors according to the information detected by these sensilla.
Regulation of the ER Stress Response by the Ion Channel Activity of the Infectious Bronchitis Coronavirus Envelope Protein Modulates Virion Release, Apoptosis, Viral Fitness, and Pathogenesis

Coronavirus (CoV) envelope (E) protein is a small structural protein critical for virion morphogenesis and release. The recently characterized E protein ion channel activity (EIC) has also been implicated in modulating viral pathogenesis. In this study, we used infectious bronchitis coronavirus (IBV) as a model to study EIC. Two recombinant IBVs (rIBVs) harboring EIC-inactivating mutations – rT16A and rA26F – were serially passaged, and several compensatory mutations were identified in the transmembrane domain (TMD). Two rIBVs harboring these putative EIC-reverting mutations – rT16A/A26V and rA26F/F14N – were recovered. Compared with the parental rIBV-p65 control, all four EIC mutants exhibited comparable levels of intracellular RNA synthesis, structural protein production, and virion assembly. Our results showed that the IBV EIC contributed to the induction of ER stress response, as up-regulation of ER stress-related genes was markedly reduced in cells infected with the EIC-defective mutants. EIC-defective mutants also formed smaller plaques, released significantly less infectious virions into the culture supernatant, and had lower levels of viral fitness in cell culture. Significantly, all these defective phenotypes were restored in cells infected with the putative EIC revertants. EIC mutations were also implicated in regulating IBV-induced apoptosis, induction of pro-inflammatory cytokines, and viral pathogenicity in vivo. Taken together, this study highlights the importance of CoV EIC in modulating virion release and various aspects of CoV – host interaction.
Modulation of lethal HPAIV H5N8 clade 2.3.4.4B infection in AIV pre-exposed mallards

ABSTRACT In 2016/2017, a severe epidemic of HPAIV H5N8 clade 2.3. 4.4 group B (H5N8B) affected Europe. To analyse the role of mallards in the spatiotemporal dynamics of global HPAIV H5N8B dispersal, mallards (Anas platyrhynchos), naturally exposed to various AIV and therefore seropositive, were challenged with H5N8B. All experiments were controlled by infection and co-housing of seronegative juvenile Pekin ducklings. All ducks that survived the first infection were re-challenged 21 dpi with the homologous H5N8B strain. After the first H5N8B infection, seropositive mallards showed only mild clinical symptoms. Moderate to low viral shedding, occurring particularly from the oropharynx and lasting for 7 days maximum, led to severe clinical disease of all contact ducklings. All challenged seronegative Pekin ducks and contact ducklings died or had to be euthanized. H5-specific antibodies were detected in surviving birds within 2 weeks. Virus and viral RNA could be isolated from several water samples until 6 and 9 dpi, respectively. Conversely, upon re-infection with homologous H5N8B neither inoculated nor contact ducklings showed any clinical symptoms, nor was an antibody titer increase of seropositive mallards or any seroconversion of contact ducklings observed. Mallard ducks naturally pre-exposed to LPAIV can play a role as a clinically unsuspicious virus reservoir for H5N8B effective in virus transmission. Mallards with homologous immunity did not contribute to virus transmission.