Miracle-dz/UniFAR-FTUs huggingface dataset:
Deregulation of mitochondrial functions provoked by long‐chain fatty acid accumulating in long‐chain 3‐hydroxyacyl‐CoA dehydrogenase and mitochondrial permeability transition deficiencies in rat heart – mitochondrial permeability transition pore opening as a potential contributing pathomechanism of 

Mitochondrial trifunctional protein and long‐chain 3‐hydroxyacyl‐CoA dehydrogenase deficiencies are fatty acid oxidation disorders biochemically characterized by tissue accumulation of long‐chain fatty acids and derivatives, including the monocarboxylic long‐chain 3‐hydroxy fatty acids (LCHFAs) 3‐hydroxytetradecanoic acid (3HTA) and 3‐hydroxypalmitic acid (3HPA). Patients commonly present severe cardiomyopathy for which the pathogenesis is still poorly established. We investigated the effects of 3HTA and 3HPA, the major metabolites accumulating in these disorders, on important parameters of mitochondrial homeostasis in Ca2+‐loaded heart mitochondria. 3HTA and 3HPA significantly decreased mitochondrial membrane potential, the matrix NAD(P)H pool and Ca2+ retention capacity, and also induced mitochondrial swelling. These fatty acids also provoked a marked decrease of ATP production reflecting severe energy dysfunction. Furthermore, 3HTA‐induced mitochondrial alterations were completely prevented by the classical mitochondrial permeability transition (mPT) inhibitors cyclosporin A and ADP, as well as by ruthenium red, a Ca2+ uptake blocker, indicating that LCHFAs induced Ca2+‐dependent mPT pore opening. Milder effects only achieved at higher doses of LCHFAs were observed in brain mitochondria, implying a higher vulnerability of heart to these fatty acids. By contrast, 3HTA and docosanoic acids did not change mitochondrial homeostasis, indicating selective effects for monocarboxylic LCHFAs. The present data indicate that the major LCHFAs accumulating in mitochondrial trifunctional protein and long‐chain 3‐hydroxyacyl‐CoA dehydrogenase deficiencies induce mPT pore opening, compromising Ca2+ homeostasis and oxidative phosphorylation more intensely in the heart. It is proposed that these pathomechanisms may contribute at least in part to the severe cardiac alterations characteristic of patients affected by these diseases.
Evaluation of HER-2/neu gene amplification and overexpression: comparison of frequently used assay methods in a molecularly characterized cohort of breast cancer specimens.

PURPOSE To compare and evaluate HER-2/neu clinical assay methods. MATERIALS AND METHODS One hundred seventeen breast cancer specimens with known HER-2/neu amplification and overexpression status were assayed with four different immunohistochemical assays and two different fluorescence in situ hybridization (FISH) assays. RESULTS The accuracy of the FISH assays for HER-2/neu gene amplification was high, 97.4% for the Vysis PathVision assay (Vysis, Inc, Downers Grove, IL) and 95.7% for the the Ventana INFORM assay (Ventana, Medical Systems, Inc, Tucson, AZ). The immunohistochemical assay with the highest accuracy for HER-2/neu overexpression was obtained with R60 polyclonal antibody (96.6%), followed by immunohistochemical assays performed with 10H8 monoclonal antibody (95.7%), the Ventana CB11 monoclonal antibody (89.7%), and the DAKO HercepTest (88.9%; Dako, Corp, Carpinteria, CA). Only the sensitivities, and therefore, overall accuracy, of the DAKO Herceptest and Ventana CB11 immunohistochemical assays were significantly different from the more sensitive FISH assay. CONCLUSION Based on these findings, the FISH assays were highly accurate, with immunohistochemical assays performed with R60 and 10H8 nearly as accurate. The DAKO HercepTest and the Ventana CB11 immunohistochemical assay were statistically significantly different from the Vysis FISH assay in evaluating these previously molecularly characterized breast cancer specimens.
The Accuracy of Endometrial Pipelle Sampling With and Without Sonographic Measurement of Endometrial Thickness

Objective: To evaluate the accuracy of Pipelle endometrial sampling with and without sonographic measurement of endometrial thickness. Methods: We studied prospectively 176 consecutive patients (23% after and 77% before menopause) scheduled for D&C. Sonographic measurement of the endometrium and endometrial biopsy with the Pipelle were performed before the curettage. Results: In 159 cases (90%), the endometrial histologic results of curettage agreed with those of the Pipelle biopsy. All three cases of endometrial cancer were identified by Pipelle aspiration. In seven cases (4%), the Pipelle aspiration failed to detect hyperplasia. Sonographic endometrial thickness of more than 5 mm slightly increased the sensitivity and slightly decreased the specificity of Pipelle aspiration from 82 to 92% and from 99 to 96%, respectively. In postmenopausal patients admitted for bleeding, the sensitivity and specificity reached 100%. Conclusions: Our data suggest that normal Pipelle aspirates in premenopausal patients with abnormal uterine bleeding are highly accurate. In postmenopausal patients with sonographic endometrial thickness of 5 mm or less, the accuracy reached 100%.
Development of Mandibular Movements Measuring System Using Double Stereo-Cameras

In this paper, we propose a 3D automated measuring system which measures the mandibular movements and the reference plane of the jaw movements. In diagnosis and treatment of the malocclusions, it is necessary to estimate the mandibular movements and the reference plane of the jaw movements. The proposed system is configured with double stereo-cameras, PC, two moving pattern plates(MPPs), two fixed pattern plates(FPPs) and one orbital marker. The virtual pattern plate is applied to calculate the homogeneous transformation matrices which describe the coordinates systems of the FPP and MPP with respect to the world coordinates system. To estimate the parameters of the hinge axis, the Euler's theorem is applied. The hinge axis points are intersections between the FPPs and the hinge axis. The coordinates of a hinge axis point with respect to the MPP coordinates system are set up to fixed value. And then, the paths of the jaw movement can be calculated by applying the homogeneous transformation matrix to fixed hinge axis point. To examine the accuracy of the measurements, experiments of measuring the hinge axis points and floating paths of them are performed using the jaw motion simulator. As results, the measurement errors of the hinge axis points are within reasonable boundary, and the floating paths are very similar to the simulator's moving path.
Cognitive neuroscience: When a rubber hand `feels' what the real hand cannot

CA,1Corresponding Author and Address WE examined a patient who was clinically much better at reporting tactile stimulation when he could see his stimulated hand. Experimentally, we found that he had dif®culty detecting taps accompanied by a salient (but not predictive) light located directly above his concealed hand. However, his performance was dramatically improved if the light was attached to a rubber hand situated in line with the patient's hidden hand. Previous studies have suggested that tactile sensitivity can be improved by nearby visual stimulation. However, our effect shows that crossmodal sensory facilitation does not only depend upon simple spatial proximity alone. Rather, a simultaneous visual event dramatically improves perception of touch speci®cally when it is attributed to the perceiver's stimulated limb. NeuroReport 10:135±138# 1999 Lippincott Williams & Wilkins.
Dual conformal strip fed cylindrical dielectric resonator antenna for circular polarization

In many circularly polarized (CP) antennas, frequency mismatching occurs between impedance and axial ratio (AR) bandwidth due to which antenna can't utilized its full bandwidth. In this paper, a conformal strip fed cylindrical dielectric resonator antenna (CDRA) with modified Wilkinson power divider is investigated to overcome the frequency mismatching problem. By connecting one new strip line in the middle of the original Wilkinson power divider, the entire AR pass band falls within the impedance pass band. This external strip increases the |Sn| bandwidth from 20.61% to 30.39% as well as shifting its frequency range in the range of the AR bandwidth so that the whole range of the AR bandwidth becomes usable. The proposed antenna provides measured |S11| and 3-dB AR bandwidth of 30.97% and 10%, respectively.
Molecular characterization of a CD95 signaling mutant.

OBJECTIVE To study the intracellular signaling events associated with ligation of the surface receptor CD95. METHODS A mutant clone of Jurkat T cells, DD3, which fails to transmit apoptotic signals through CD95, was selected for study. Surface expression of CD95 and the primary nucleotide sequence of CD95, as well as the functional effects of a mutant CD95 molecule found in DD3, were examined. RESULTS DD3, while exhibiting impaired ability to undergo apoptosis after CD95 ligation, retained the ability to die after ultraviolet light stimulation. A CD95 complementary DNA (cDNA) cloned from DD3 encoded a mutant transmembrane protein lacking the carboxy-terminal "death domain. " Western blotting confirmed the presence of both wild-type and mutant CD95 protein in DD3. Transfection of the mutant CD95 cDNA into parental Jurkat cells conferred protection from CD95-mediated apoptosis. CONCLUSION A mutant CD95 receptor lacking the cytoplasmic "death domain" can interfere with wild-type receptor function in T cells.
Adjuvant therapy for breast cancer: practical lessons from the early breast cancer trialists' collaborative group.

Increased early detection and improved treatment have made clinical cure possible for more patients with breast cancer than ever before. The medical oncologist contributes to this outcome through participation in the multidisciplinary care of patients with early stage disease and by providing systemic adjuvant therapy designed to treat undetectable metastatic foci of disease. One’s approach can be colored by place of training, ongoing research interests, and other biases based on data, anecdote, culture, philosophy, or even local economy. Hence, the approach to systemic treatment for patients with resectable breast cancer can vary widely. However, post-operative systemic therapy (“adjuvant treatment”) is clearly effective and no doubt contributes to the falling mortality seen in many parts of the world over the past decade. This chapter reviews the metaanalyses performed every fifth year at Oxford University to provide a foundation for clinical practice, clinical trials, and for other chapters in this textbook.
Humeral Retrotorsion and Glenohumeral Motion in Youth Baseball Players Compared With Age-Matched Nonthrowing Athletes

Background: Baseball players exhibit a more posteriorly oriented humeral head in their throwing arm. This is termed humeral retrotorsion (HRT) and likely represents a response to the stress of throwing. This adaptation is thought to occur while the athlete is skeletally immature, however currently there is limited research detailing how throwing activity in younger players influences the development of HRT. In addition, it is presently unclear how this changing osseous orientation may influence shoulder motion within young athletes. Purpose: To determine the influence of throwing activity and age on the development of side-to-side asymmetry in HRT and shoulder range of motion (ROM). Study Design: Cross-sectional study; Level of evidence, 3. Methods: Healthy athletes (age range, 8-14 years) were categorized into 2 groups based upon sports participation; throwers (n = 85) and nonthrowers (n = 68). Bilateral measurements of HRT, shoulder external rotation (ER), internal rotation (IR), and total range of motion (TROM) at 90° were performed using diagnostic ultrasound and a digital inclinometer. Side-to-side asymmetry (dominant minus nondominant side) in HRT and in shoulder ER, IR, and TROM were assessed. Statistical analysis was performed with 2-way analysis of variance and Pearson correlation coefficients. Results: Throwers demonstrated a larger degree of HRT on the dominant side, resulting in greater asymmetry compared with nonthrowers (8.7° vs 4.8°). Throwers demonstrated a gain of ER (5.1°), a loss of IR (6.0°), and no change in TROM when compared with the nondominant shoulder. Pairwise comparisons identified altered HRT and shoulder ROM in all age groups, including the youngest throwers (age range, 8-10.5 years). A positive correlation existed between HRT and ER ROM that was stronger in nonthrowers (r = 0.63) than in throwers (r = 0.23), while a negative correlation existed with IR that was stronger in throwers (r = −0.40) than in nonthrowers (r = −0.27). Conclusion: Throwing activity causes adaptive changes in HRT and shoulder ROM in youth baseball players at an early age. Other factors in addition to HRT influence shoulder motion within this population.
Measurement of rapidly available glucose (RAG) in plant foods: a potential in vitro predictor of the glycaemic response

Abstract The glycaemic index (GI) is an in vivo measurement based on the glycaemicresponse to carbohydrate-containing foods, and allows foods to be ranked on the basis of the rate of digestion and absorption of the carbohydrates that they contain. GI values are normalizedto a reference amount of available carbohydrate and do not reflect the amounts of carbohydrate normally present in foods; for example, a food with a low content of carbohydrates will have a high GI value if that carbohydrate is digested and absorbed rapidly in the human small intestine. This is potentially confusing for a person wishing to control his or her blood glucoselevels by the choice of foods. The rate and extent of starch digestion in vitro has been measured using a technique that classifies starch into three major fractions: rapidly digestible starch (RDS), slowly digestible starch (SDS) and resistant starch (RS). In addition, thistechnique gives a value for rapidly available glucose (RAG), which includes RDS, free glucose and the glucose moiety of sucrose. When the values for thirty-nine foods were expressed on the basis ofthe available carbohydrate content of these foods, highly significant (P<0·001) positive correlations were observed between GI and both RDS and RAG. The measurement of RAGin vitro provides values for direct calculation of the amount of glucose likely to be rapidly absorbed in the human small intestine and,thus, to influence blood glucose and insulin levels. These values can be used to compare foods, as eaten,on an equal-weight basis. Food-table RAG values would allow simple calculation of the total amount of RAG provided by single foods, by whole meals and by whole diets. Studies are planned in which RAG and the glycaemic response in man will be measured for identical food products.
Towards designing telepresence robot navigation for people with disabilities

Purpose – The authors believe that people with cognitive and motor impairments may benefit from using of telepresence robots to engage in social activities. To date, these systems have not been designed for use by people with disabilities as the robot operators. The paper aims to discuss these issues. Design/methodology/approach – The authors conducted two formative evaluations using a participatory action design process. First, the authors conducted a focus group (n=5) to investigate how members of the target audience would want to direct a telepresence robot in a remote environment using speech. The authors then conducted a follow-on experiment in which participants (n=12) used a telepresence robot or directed a human in a scavenger hunt task. Findings – The authors collected a corpus of 312 utterances (first hand as opposed to speculative) relating to spatial navigation. Overall, the analysis of the corpus supported several speculations put forth during the focus group. Further, it showed few statistic.
Morphological changes among hippocampal dentate granule cells exposed to early kindling‐epileptogenesis

Temporal lobe epilepsy is associated with changes in the morphology of hippocampal dentate granule cells. These changes are evident in numerous models that are associated with substantial neuron loss and spontaneous recurrent seizures. By contrast, previous studies have shown that in the kindling model, it is possible to administer a limited number of stimulations sufficient to produce a lifelong enhanced sensitivity to stimulus evoked seizures without associated spontaneous seizures and minimal neuronal loss. Here we examined whether stimulation of the amygdala sufficient to evoke five convulsive seizures (class IV or greater on Racine's scale) produce morphological changes similar to those observed in models of epilepsy associated with substantial cell loss. The morphology of GFP‐expressing granule cells from Thy‐1 GFP mice was examined either 1 day or 1 month after the last evoked seizure. Interestingly, significant reductions in dendritic spine density were evident 1 day after the last seizure, the magnitude of which had diminished by 1 month. Further, there was an increase in the thickness of the granule cell layer 1 day after the last evoked seizure, which was absent a month later. We also observed an increase in the area of the proximal axon, which again returned to control levels a month later. No differences in the number of basal dendrites were detected at either time point. These findings demonstrate that the early stages of kindling epileptogenesis produce transient changes in the granule cell body layer thickness, molecular layer spine density, and axon proximal area, but do not produce striking rearrangements of granule cell structure. © 2013 Wiley Periodicals, Inc.
Inhaled treprostinil sodium for the treatment of pulmonary arterial hypertension

Introduction: Inhaled treprostinil sodium, a prostacyclin analog, is the most recent agent to receive FDA approval for the treatment of a fatal orphan disease: pulmonary arterial hypertension (PAH). Areas covered: This article first reviews the data supporting the use of infusion prostacyclin and treprostinil as treatments for PAH. The authors then review inhaled treprostinil sodium: the compound and its properties, initial clinical evidence supporting its use and the pivotal data that support a role for inhaled treprostinil sodium in the treatment of patients with PAH. A broad PubMed literature search was done to identify the most current data on the use of treprostinil for PAH. Inhaled treprostinil received FDA approval to improve exercise tolerance in 2009, following the publication of several studies demonstrating its safety and its beneficial effect on hemodynamics, exercise capacity and quality-of-life measures. Expert opinion: Inhaled treprostinil seems to have a similar efficacy profile as inhaled iloprost, although the demonstrated trough effect on exercise tolerance with treprostinil is an advantage. Perhaps more importantly, the longer half-life makes treprostinil more convenient with four-times-daily dosing. As compared with iloprost, inhaled treprostinil has practical advantages for patients (less frequent dosing, shorter inhalation times, once-daily preparation of the drug delivery device, and easier routine maintenance of the nebulizer), but direct comparisons about efficacy or durability of the treatment effect cannot be made in the absence of carefully controlled trials.
Serum BNP Concentration and Left Ventricular Mass in CAPD and Automated Peritoneal Dialysis Patients

Objective To compare ultrafiltration under continuous ambulatory peritoneal dialysis (CAPD) and automated PD (APD), disclosing potential effects on serum B-type natriuretic peptide (BNP) levels and echocardiographic findings. Patients and Methods This cross-sectional clinical study included 32 patients on CAPD and 30 patients on APD without clinical evidence of heart failure or hemodynamically significant valvular heart disease. Peritoneal equilibration tests, BNP levels, and echocardiographic measurements were performed in each subject. BNP measurements were also performed in 24 healthy control subjects. Results Patients on APD had lower ultrafiltration and higher values of BNP and left ventricular mass index (LVMI) compared with patients on CAPD (respectively: 775 ± 160 vs 850 ± 265 mL, p = 0.01; 253.23 ± 81.64 vs 109.42 ± 25.63 pg/mL, p = 0.001; 185.12 ± 63.50 vs 129.30 ± 40.95 g/m2, p = 0.001). This occurred despite higher mean dialysate glucose concentrations and far more extensive use of icodextrin in the APD group. Conclusion Treatment with APD is associated with higher plasma BNP levels and LVMI compared to CAPD. This may be the result of chronic fluid retention caused by lower ultra-filtration in APD patients.
The influence of the gut microflora on the digestion of dietary and endogenous proteins: studies of the amino acid composition of the excreta of germ-free and conventional chicks

1. To assess the part played by the microflora in the digestion of proteins, the amino acid composition of the excreta and the apparent and true digestibilities of individual amino acids were measured in germ-free (GF) and conventional (CV) chicks. 2. Three diets were used: diet 1, nitrogen-free; diet 2,280 g protein/kg as (g/kg): casein 80, gelatin 100 and freeze-dried egg albumen 100 and diet 3,280 g protein/kg as diet 2 but with heat-damaged instead of freeze-dried egg albumen. Half of the GF and half of the CV chicks received the N-free diet in the first 7 d of test and the other half of each group received either diet 2 or diet 3. In the second 7 d test period those chicks which had been given the N-free diet received either diet 2 or diet 3 while those which had been given protein diets received the N-free diet. Total amino acids were measured in hydrolysates of the soluble and insoluble fractions of the excreta collected in the last 3 d of each test period. 3. The amino acid composition of the soluble fraction of the excreta of chicks given either diet 2 or diet 3 differed markedly from that of chicks given the N-free diet. The amino acid composition of the insoluble fraction of the excreta of chicks given diet 2 was similar to that of chicks given the N-free diet, whereas that of chicks given diet 3 was markedly different and resembled egg albumen in composition. 4. In the soluble fraction of excreta from CV chicks given diets 1 and 2 the proportions of threonine, serine and glucosamine were lower and those of methionine, leucine, isoleucine and phenylalanine were higher than in those from GF chicks, particularly on diet 1. In the insoluble fraction of excreta from CV chicks given these two diets, compared with GF chicks, there were lower proportions of serine and proline and higher proportions of cysteic acid and lysine, the latter particularly with diet 1. 5. Lower proportions of threonine, serine and glucosamine were also observed in the soluble fractions of excreta from the CV chicks given diets 1 and 3, compared with GF chicks, whereas the proportion of glutamic acid was higher. With these two diets the insoluble fraction from CV chicks contained a higher proportion of alanine. In each instance the environmental effect was greater with diet 1. 6. No effect of environment on either apparent or true digestibility of individual dietary protein amino acids was demonstrated, with the exception of threonine in diet 2, the true digestibility of which was higher in GF than in CV chicks. 7. It was concluded that the gut microflora of the chick had little influence on the digestion of the proteins in the diets tested, but may serve an important role in the degradation of endogenous proteins and the recycling of N.