Miracle-dz/UniFAR-FTUs huggingface dataset:
The Leptospiral Major Outer Membrane Protein LipL32 Is a Lipoprotein Expressed during Mammalian Infection

ABSTRACT We report the cloning of the gene encoding the 32-kDa lipoprotein, designated LipL32, the most prominent protein in the leptospiral protein profile. We obtained the N-terminal amino acid sequence of a staphylococcal V8 proteolytic-digest fragment to design an oligonucleotide probe. A Lambda-Zap II library containingEcoRI fragments of Leptospira kirschneri DNA was screened, and a 5.0-kb DNA fragment which contained the entire structural lipL32 gene was identified. Several lines of evidence indicate that LipL32 is lipid modified in a manner similar to that of other procaryotic lipoproteins. The deduced amino acid sequence of LipL32 would encode a 272-amino-acid polypeptide with a 19-amino-acid signal peptide, followed by a lipoprotein signal peptidase cleavage site. LipL32 is intrinsically labeled during incubation of L. kirschneri in media containing [3H]palmitate. The linkage of palmitate and the amino-terminal cysteine of LipL32 is acid labile. LipL32 is completely solubilized by Triton X-114 extraction of L. kirschneri; phase separation results in partitioning of LipL32 exclusively into the hydrophobic, detergent phase, indicating that it is a component of the leptospiral outer membrane. CaCl2 (20 mM) must be present during phase separation for recovery of LipL32. LipL32 is expressed not only during cultivation but also during mammalian infection. Immunohistochemistry demonstrated intense LipL32 reactivity withL. kirschneri infecting proximal tubules of hamster kidneys. LipL32 is also a prominent immunogen during human leptospirosis. The sequence and expression of LipL32 is highly conserved among pathogenic Leptospira species. These findings indicate that LipL32 may be important in the pathogenesis, diagnosis, and prevention of leptospirosis.
A HYBRID MULTILEVEL VOLTAGE SOURCE CONVERTER FOR FAULT TOLERANT IN HVDC TRANSMISSION SYSTEM

This paper proposes a new breed of high voltage dc (HVDC) transmission systems based on a hybridmultilevel voltage source converter (VSC) with ac-side cascaded H-bridge cells. The proposed HVDC system offers the operational flexibility of VSCbased systems in terms of active and reactive power control, blackstart capability, in addition to improved ac fault ride-through capability and the unique feature of current-limiting capability during dc side faults. Additionally, it offers features such as smaller footprint and a larger active and reactive power capability curve than existing VSC-basedHVDC systems, including those usingmodular multilevel converters. To illustrate the feasibility of the proposed HVDC system, this paper assesses its dynamic performance during steady-state and network alterations, including its response to ac and dc side faults.
Table Olives: A Vehicle for the Delivery of Bioactive Compounds

Table olives and olive oil are sources of compounds with important biological properties and essential components of the Mediterranean Diet. For a long period the scientific community focused its attention mainly on the beneficial effects of olive oil on health. The importance of the edible processed fruit was rather overlooked. Table olives contain a large amount of high quality fat, essential amino acids, minerals, vitamins, fiber and a group of minor compounds, which include biophenols and pentacyclic triterpenes. In the last decade an effort is seen in the literature to re-evaluate table olives as sources of bioactive compounds. There is an impressive increase of papers dealing with the composition of processed olives, the effect of variety and processing methods on the level of antioxidants and other minor bioactive constituents, and generally the improvement of healthy features. The information, however, is rather scattered and not all the publications are of the same scientific level. There are many repetitions but also discrepancies and blanks due to difficulties deriving from the complex nature of phenols, the biochemical routes related to lactic acid and other fermenting bacteria and the analytical results, considering the lack of standard methods for the quantitation of small or minute quantities of bioactive compounds The present review discusses table olives as a vehicle for the delivery of bioactive compounds. It concentrates mainly on the levels of bioactive compounds in relation to the treatment of olive fruits and the expected healthy features and functionality.
On the Estimation of Slow-Fading Coefficients for Pilot Contamination Precoding

Massive multiple-input multiple-output (MIMO) has been actively studied for next generation wireless systems since it can provide high data rate and an improved energy efficiency in multiuser systems using large antenna arrays at base stations (BSs). While massive MIMO has various advantages, its perfor- mance is known to be limited by pilot contamination. To mitigate the intercell interference resulting from pilot contamination, pilot contamination precoding (PCP) was proposed for downlink transmissions. For PCP, slow-fading coefficients need to be estimated. In this paper, we study the estimation of slow-fading coefficients. We show that slow-fading coefficients cannot be estimated using the conventional uplink training. Thus, we have proposed amplitude modulated pilot sequences, which allow us to estimate slow-fading coefficients without requiring additional orthogonal pilot sequences.
Natural antibiotic susceptibility of Listeria species: L. grayi, L. innocua, L. ivanovii, L. monocytogenes, L. seeligeri and L. welshimeri strains.

OBJECTIVE To investigate the natural susceptibility to 71 antimicrobial agents of 103 Listeria strains belonging to all known Listeria species (L. monocytogenes (N = 21), L. innocua (N = 21), L. seeligeri (N = 21), L. ivanovii (N = 19), L. welshimeri (N = 11), and L. grayi (N = 10)). METHODS MICs were determined using a microdilution procedure in H-Medium. RESULTS All listeriae were naturally sensitive or intermediate to tetracyclines, aminoglycosides, penicillins (except oxacillin), loracarbef, cefazoline, cefaclor, cefotiam, cefoperazone, carbapenems, macrolides, lincosamides, glycopeptides, dalfopristin/quinupristin, chloramphenicol and rifampicin (probably except L. grayi). Listeria spp. were naturally resistant or intermediate to most 'modern' cephalosporins (cefetamet, cefixime, ceftibuten, ceftazidime, cefdinir, cefpodoxime, cefotaxime, ceftriaxone, cefuroxime), aztreonam, pipemidic acid, dalfopristin quinupristin and sulfamethoxazole. Significant differences in natural susceptibility among the species were seen with the quinolones, trimethoprim, co-trimoxazole, rifampicin, fosfomycin and fusidic acid. It seems likely that L. grayi is naturally resistant to all antifolates; the species was least susceptible to rifampicin and most susceptible to quinolones, whereas L. ivanovii was naturally resistant to most quinolones. L. ivanovii was naturally sensitive to fosfomycin, whereas L. innocua and L. monocytogenes were naturally resistant. L. ivanovii was also the most susceptible species to fusidic acid. CONCLUSIONS The present study describes a database on the natural susceptibility of Listeria spp. to a wide range of antibiotics, which can be used to validate susceptibility testing results of these microorganisms.
A Heme Oxygenase-1 Transducer Model of Degenerative and Developmental Brain Disorders

Heme oxygenase-1 (HO-1) is a 32 kDa protein which catalyzes the breakdown of heme to free iron, carbon monoxide and biliverdin. The Hmox1 promoter contains numerous consensus sequences that render the gene exquisitely sensitive to induction by diverse pro-oxidant and inflammatory stimuli. In “stressed” astroglia, HO-1 hyperactivity promotes mitochondrial iron sequestration and macroautophagy and may thereby contribute to the pathological iron deposition and bioenergetic failure documented in Alzheimer disease, Parkinson disease and certain neurodevelopmental conditions. Glial HO-1 expression may also impact neuroplasticity and cell survival by modulating brain sterol metabolism and the proteasomal degradation of neurotoxic proteins. The glial HO-1 response may represent a pivotal transducer of noxious environmental and endogenous stressors into patterns of neural damage and repair characteristic of many human degenerative and developmental CNS disorders.
Future Scope of Internet Shopping in India

This paper analyzes the impact of e-commerce on market where established firms cage competition frominternet based entrants with focused offerings. An innovative product with societal acceptance is the onethat aids the comfort, convenience and efficiency in everyday life. Purchasing and shopping at big mallsis becoming daily activity in metro cities. We can see big rush at these malls on holidays and weekends.People purchase different items and put them in trolley. After completion of purchases, one needs to go tobilling counter for payments. At billing counter the cashier prepare the bill using bar code reader whichis very time consuming process and results in long queue at billing counter. In this paper, we discuss aproduct “Intelligent Shopping Cart” being developed to assist a person in everyday shopping in terms ofreduced time spent while purchasing. The main objective of proposed system is to provide a technologyoriented, low-cost, easily scalable, and rugged system for assisting shopping in person. New Informationtechnology has taken important place in the future development of financial services, especially bankingsector transition are affected more than any other financial provider groups. Increased use of mobileservices and use of internet as a new distribution channel for banking transactions and internationaltrading requires more attention towards e-banking security against fraudulent activities.
Online or offline: Perceived risk and its influence on young consumers decision of where to consume.

This paper seeks to increase the knowledge about how perceived risk influence young consumers’ decision to consume online or in a brick-and-mortar store. In addition, gain a deeper understanding if brick-and-mortar stores are still relevant when online shopping is taking a more prominent role in how individuals consume their products and services. The conducted research analyses different factors of perceived risk and the part they play in young consumers’ decision of where to purchase products and services. Data was gathered through semi-structured interviews with respondents between the age of 18 30 who actively consume products and services both online and in a brick-and-mortar store. The findings indicate that financial, functional and convenience risk are prominent factors influencing young consumers’ decision to either consume online or in a brick-and-mortar store. While factors such as non-delivery and privacy risk play minor parts. Supervisor: Peter Zackariasson
An Estimate of the U.S. Government’s Undercount of Nonfatal Occupational Injuries

Learning ObjectivesEstimate how many workers—and in which occupations—are presently excluded from the Bureau of Labor Statistics Annual Survey of Occupational Illnesses and Injuries.Consider possible reasons for under-reporting of injuries by private firms and their employees.Provide the best estimate of the degree of under-reporting, and note the possible consequences for workers’ health. Debate surrounds the size of the underestimate of nonfatal occupational injuries produced by the U.S. Bureau of Labor Statistics (BLS). We developed models that separated categories of injuries: BLS Annual Survey, federal government, agriculture, state and local government, self-employed outside agriculture, and all other. The models generated varying estimates depending on the assumptions for each category pertaining to job risks and amount of underreporting. We offered justification for the assumptions based on published studies as well as our own analyses of BLS data. The models suggested the Annual Survey missed from 0% to 70% of the number of injuries (from private firms, excluding the self-employed) it was designed to capture. However, when we included firms and governments the Annual Survey was not designed to capture, and considered reasonable assumptions regarding underreporting, we estimated the BLS missed between 33% and 69% of all injuries. We concluded that there was substantial undercapture in the BLS Annual Survey, some due to the excluded categories of government workers and the self-employed, as well as some due to underreporting.
National practice patterns and time trends in androgen ablation for localized prostate cancer.

BACKGROUND Recent reports have suggested that growing numbers of patients with localized prostate cancer are receiving androgen deprivation therapy as primary or neoadjuvant treatment, yet sparse clinical evidence supports the use of such treatment except among patients with high-risk or locally advanced disease receiving external beam radiotherapy. We describe national trends in the use of androgen deprivation therapy for localized disease. METHODS CaPSURE is an observational database of 7195 patients with prostate cancer. This study included 3439 of these patients who were diagnosed since 1989, had clinical staging information available, and were treated with radical prostatectomy, radiation therapy, or primary androgen deprivation therapy (PADT). High-, intermediate-, and low-risk groups were defined by serum prostate-specific antigen level, Gleason score, and clinical tumor stage. Time trends in the use of PADT and neoadjuvant androgen deprivation therapy (NADT) were analyzed. All statistical tests were two-sided. RESULTS Rates of PADT use rose sharply between 1989 and 2001, from 4.6% (95% confidence interval [CI] = 3.4% to 5.8%) to 14.2% (95% CI = 12.2% to 16.2%), from 8.9% (95% CI = 7.3% to 10.5%) to 19.7% (95% CI = 17.5% to 21.9%), and from 32.8% (95% CI = 29.9% to 35.7%) to 48.2% (95% CI = 45.1% to 51.3%) (all P<.001) in low-, intermediate-, and high-risk groups, respectively. NADT use also increased in association with radical prostatectomy (2.9% [95% CI = 2.1% to 3.7%] to 7.8% [95% CI = 6.5% to 9.1%] of patients, P =.003) and external beam radiotherapy (9.8% [95% CI = 7.5% to 12.1%] to 74.6% [95% CI = 70.8% to 78.4%], P<.001) across all risk levels combined. Rates of NADT use among patients treated with brachytherapy also increased but not statistically significantly (7.4% [95% CI = 3.5% to 11.3%] to 24.6% [95% CI = 18.2% to 31.0%], P =.100). CONCLUSIONS Rates of both PADT and NADT are increasing across risk groups and treatment types. Future clinical trials must define more clearly the appropriate role of hormonal therapy in localized prostate cancer, and their results should shape updated practice guidelines.
Essential business process modeling

Ten years ago, groupware bundled with email and calendar applications helped track the flow of work from person to person within an organization. Workflow in today's enterprise means more monitoring and orchestrating massive systems. A new technology called Business Process Management, or BPM, helps software architects and developers design, code, run, administer, and monitor complex network-based business processesSound complicated? It is. But it's downright frustrating when you have to search the Web for every little piece of information vital to the process. Essential Business Process Modeling gathers all the concepts, design, architecture, and standard specifications of BPM into one concise book, and offers hands-on examples that illustrate BPM's approach to process notation, execution, administration and monitoring.Author Mike Havey demonstrates standard ways to code rigorous processes that are centerpieces of a service-oriented architecture (SOA), which defines how networks interact so that one can perform a service for the other. His book also shows how BPM complements enterprise application integration (EAI), a method for moving from older applications to new ones, and Enterprise Service BUS for integrating different web services, messaging, and XML technologies into a single network. BPM, he says, is to this collection of services what a conductor is to musicians in an orchestra: it coordinates their actions in the performance of a larger composition.Essential Business Process Modeling teaches you how to develop examples of process-oriented applications using free tools that can be run on an average PC or laptop. You'll also learn about BPM design patterns and best practices, as well as some underlying theory. The best way to monitor processes within an enterprise is with BPM, and the best way to navigate BPM is with this valuable book. BPM replaces those sketchy flowchart diagrams that business analysts draw on whiteboards with a precise model that uses standard graphical and XML representations, and an architecture that allows it converse with other services, systems, and users.
Oncology drug development: what is changing?

Drug development is a long and expensive process that, on average, takes about 13 years from drug discovery to approval by the U.S. Food and Drug Administration (FDA) for patient use (IMS Health, 2004). Cancer drugs cost more to develop than other types of drugs because of the absence of preclinical systems that can accurately predict the efficacy and toxicity of new agents and the increasing complexity of clinical trials that involve molecularly targeted agents and advanced technologies (Kummar, 2007). Developing a new cancer drug is estimated to cost $1.042 billion compared to $848 million for other drug types (DiMasi & Grabowski, 2007). Data from 1991–2000 show that only about 5% of potential cancer drugs are approved for patient use (Kola & Landis, 2004) (see Figure 1). This high rate of failure in cancer drug development compounded by the high cost of research has set the stage for a new approach in developing cancer drugs: phase 0 trials.
An improved technique for the study of immunofluorescence using non-decalcified frozen guinea pig cochlea

Summary A technique for producing frozen sections from the non-decalcified guinea pig cochlea is described. The sections can be utilised or various immunofluorescent studies, including the detection of humoral anti-bodies against different cellular elements in patients with cochleo-vestibular disorders. The technique assures the preservation of the cochlear architecture and the tissue antigenicity, and it avoids damage to the fine cochlear structural details.
Research on supply chain revenue-sharing contract with a loss-averse retailer

Based on the prospect theory, this paper studies the revenue-sharing contract with stock-out cost of supply chain with a loss-averse retailer and a risk-neutral supplier. The order behaviors of a loss-averse retailer in the wholesale price contract and the revenue-sharing contract were studied respectively. It finds, with stock-out cost, the optimized order quantity of the loss-averse retailer may deviate from the optimized quantity of the centralized system. However, the revenue-sharing contract can improve the performance of supply chain.
Implant Microdesigns and Their Impact on Osseointegration

Researchers are in constant search for the ideal implant surface condition in an attempt to improve osseointegration of the implant and to increase bone-to-implant contact. Although introduction of rough surfaces was able to overcome a lot of limitations that presented with machined surfaces, they are still incapable of ensuring predictable results and guaranteed success. Various techniques in implant surface modifications have been proposed, all aimed at improving bone formation around dental implants. Understanding how surface modification of dental implants can impact osseointegration might help the clinician to maximize the success rate of implants and diminish the complications that can be encountered after their placement. In view of that, this article is aimed at assessing the different surface conditioning techniques available and will present a review of the literature that focuses on the influence of microdesign of dental implants on their osseointegration.
“Total therapy” studies of acute lymphocytic leukemia in children. Current results and prospects for cure

The ultimate goal of “total therapy” studies has been to establish a significant cure rate for childhood acute lymphocytic leukemia. The principle features of these studies have included administration of maximum tolerated combination chemotherapy and “prophylactic” central nervous system (CNS) irradiation. In the first three studies, a 17% 7‐year leukemia‐free remission rate was attained. The number of patients in complete remission decreased exponentially until 2 to 3 years, but stabilized thereafter. Complete remission duration was the earliest and most accurate indicator of long‐term leukemia‐free remission. In Study V, more aggressive CNS therapy was given and over half the patients have been in continuous complete remission for 3 1/2 to 4 years. Similar results are emerging in subsequent studies which also employ aggressive “prophylactic” CNS therapy. Therapy was stopped after 2 or more years of complete remission in 42 patients in “total therapy” studies, and nine have relapsed. Six of the nine had not received “prophylactic” CNS irradiation. If current trends in these studies continue, we can expect a 50% long‐term leukemia‐free remission rate. Thus, with currently available modalities of therapy, the prospects for cure appear bright.
The Role of Compulsive Texting in Adolescents’ Academic Functioning

Text messaging has increased dramatically among adolescents over the past 10 years. Many researchers have cited potential consequences associated with a high frequency of texting and problematic texting behaviors. This study examines the relations among frequency of texting, a specific type of problematic texting (i.e., compulsive texting), and adolescents’ academic achievement and attitudes about school. Adolescents in 8th (n = 211) and 11th (n = 192) grades participated in this study. Results indicated that, as hypothesized, teens’ compulsive texting was significantly positively related to their frequency of texting and negatively related to their grades, school bonding, and perceived scholastic competence. It is noteworthy that the negative relation between compulsive texting and academic functioning held true only for females and not for males. Actively preventing or reducing compulsive texting may ameliorate the potential effects of texting on academic adjustment in adolescents.
Modelado numérico de un colector solar térmico aplicado a buques de crucero y ferrys

En esta investigacion se aborda el modelado numerico de un nuevo diseno de colector solar termico y se validan los resultados mediante un estudio experimental. Asimismo, se propone la implementacion de este colector solar en el sector maritimo y, para ello, se evalua su utilizacion con el fin de obtener agua caliente en buques tipo crucero y ferrys. En la parte de simulacion se presenta una metodologia que puede ser utilizada para otras topologias de captador y se obtienen los valores que caracterizan al captador. En la parte correspondiente al estudio experimental se construye un prototipo del nuevo colector, se comparan los resultados de los ensayos con la simulacion y los experimentales obtenidos para dos colectores comerciales, poniendo de manifiesto su alto rendimiento. En la parte de la aplicacion se propone una posible integracion estructural, en los buques objeto de nuestro estudio, y se muestra como estos nuevos colectores ayudan a reducir el consumo de combustible y las emisiones de CO2.