Miracle-dz/UniFAR-FTUs huggingface dataset:
Monitoring and quantifying dynamic physiological processes in live neurons using fluorescence recovery after photobleaching

The direct visualization of subcellular dynamic processes is often hampered by limitations in the resolving power achievable with conventional microscopy techniques. Fluorescence recovery after photobleaching has emerged as a highly informative approach to address this challenge, permitting the quantitative measurement of the movement of small organelles and proteins in living functioning cells, and offering detailed insights into fundamental cellular phenomena of physiological importance. In recent years, its implementation has benefited from the increasing availability of confocal microscopy systems and of powerful labeling techniques based on genetically encoded fluorescent proteins or other chemical markers. In this review, we present fluorescence recovery after photobleaching and related techniques in the context of contemporary neurobiological research and discuss quantitative and semi‐quantitative approaches to their interpretation.
Factor Analysis and the Retrieval of Medical Images Depicting Structures with Similar Shapes

This work presents a new perspective to medical image retrieval based on factor analysis. The shape of anatomical structures are represented as high-dimensional sets of vector variables obtained from non-rigidly deforming a template image so as to align its anatomy with the subject anatomy of a group. By eliminating the redundancy embedded in the data, a reduced set of factors is determined, corresponding to new variables with possible anatomic significance. The method’s ability to retrieve relevant images is exemplified in a study of the corpus callosum, a structure with very subtle shape differences. The factor analysis approach is compared to principal component analysis in a set of 960 experiments, yielding significantly higher precision rates.
Microlaser: A laser with one atom in an optical resonator.

We have demonstrated laser oscillation with one atom in an optical resonator. In our experiment a beam of $^{138}\mathrm{Ba}$ atoms traverses a single-mode cavity with a finesse of 8 \ifmmode\times\else\texttimes\fi{} ${10}^{5}$. The atoms are excited by a $\ensuremath{\pi}$ pulse from the $^{1}S_{0}$ ground state to the $^{3}P_{1}(m=0)$ excited state before they enter the cavity. Laser oscillation at 791 nm ($^{3}P_{1}\ensuremath{\rightarrow}^{1}S_{0}$) has been observed, with the mean number of atoms inside the mode ranging from 0.1 to 1.0, resulting in the mean number of photons inside the cavity changing from 0.14 to 11. The results are in good agreement with a fully quantized micromaser theory adapted to our experiment.
Review of Pancreatic Lesions in Von Hippel-Lindau Disease

Von Hippel-Lindaudisease (VHLD) is a rare hereditary tumor syndrome, inherited in autosomal dominant manner. Patients diagnosed with VHLD have a high risk of developing neoplasms of various organs (eyes, central nervous system, bone, kidney, adrenal glands, epididymis, broad ligament and pancreas). Due to its rarity, most of the physicians fail to properly diagnose it in time, and they might expose patients to a risk of unnecessary surgeries with important consequences in a long run. When this condition is diagnosed, lifelong follow-up is necessary. Pancreatic involvement it is seen in most patients with VHLD and various pancreatic lesions have been described, including cystic lesions (simple unilocular or serous microcystic or macro/micro-cystic adenomas), neuroendocrine tumors (NET), renal cell cancer metastasis and adenocarcinoma.
Interbasin Underflow Between Closed Altiplano Basins in Chile

Interbasin ground water movement of 200 to 240 L/sec occurs as underflow beneath a mountainous surface water divide separating the topographically higher Salar de Michincha from the topographically lower Salar de Coposa internally drained basins in the Altiplano of northern Chile. Salt‐encrusted flats (salars) and saline lakes occur on the lowest parts of the basin floors and comprise the principal evaporative discharge areas for the basins. Because a surface water divide separates the basins, surface water drainage boundaries do not coincide with ground water drainage boundaries. In the region, interbasin ground water movement is usually not recognized, but occurs for selected basins, and at places is an important component of ground water budgets. With increasing development of water for mining industry and potential exportation of ground water from the Altiplano for use at coastal cities, demonstration and quantification of interbasin movement is important for assessment of sustainable ground water development in a region of extreme aridity. Recognition and quantification of interbasin ground water underflow will assist in management of ground water resources in the arid Chilean Altiplano environment.
A Modification of the Jaccard–Tanimoto Similarity Index for Diverse Selection of Chemical Compounds Using Binary Strings

Determination of molecular similarity plays an important role in analyzing large compound databases in chemical and pharmaceutical research. When molecules are described by binary vectors with bits corresponding to the presence or absence of structural features, the Tanimoto association coefficient is the most commonly used measure of similarity or chemical distance between two compounds. However, when used to select compounds for an optimal spread design, the Tanimoto coefficient produces an intrinsic bias toward smaller compounds. We have developed a new association coefficient that overcomes this bias. This article gives details of the new coefficient and contrasts the two coefficients for selecting diverse sets of compounds from a large collection. When the Tanimoto coefficient is modified as suggested to select a diverse set in the National Cancer Institute and Registry of Toxic Effects of Chemical Substances databases, the average number of features among the selected compounds increases by more than 50%.
Listening for prescriptions: a national consultation on pharmaceutical policy issues.

OBJECTIVES AND METHODS Pharmaceutical policy is an increasingly costly, essential and challenging component of health system management. We sought to identify priority pharmaceutical policy issues in Canada and to translate them into research priorities using key informant interviews, stakeholder surveys and a deliberative workshop. RESULTS WE FOUND CONSENSUS ON OVERARCHING POLICY GOALS: to provide all Canadians with equitable and sustainable access to necessary medicines. We also found widespread frustration that many key pharmaceutical policy issues in Canada - including improving prescription drug financing and pricing - have been persistent challenges owing to a lack of policy coordination. The coverage of extraordinarily costly medicines for serious conditions was identified as a rapidly emerging policy issue. CONCLUSION Targeted research and knowledge translation activities can help address key policy issues and, importantly, challenges of policy coordination in Canada and thereby reduce inequity and inefficiency in policy approaches and outcomes.
Proton pump inhibitors: a survey of prescribing in an Irish general hospital

Prescription rates and attendant costs of proton pump inhibitors (PPIs) continue to rise. The aim of this study was to assess the extent and appropriateness of PPI prescribing in an Irish general hospital. Using a structured pro forma, we conducted a 1‐day comprehensive survey of the prescription charts of all inpatients. Of the 157 inpatients, 48 (30.6%) were on PPI therapy and omeprazole was the most widely prescribed PPI. Rabeprazole, the least expensive PPI, was the least prescribed. Prescription of PPI therapy was for an approved indication in 32 patients (66.6%) but was for an unapproved or unknown indication in 16 (33.3%). Prescribing had been initiated in hospital in 34 patients (70.8%) but only one‐third of the patients on PPI therapy had undergone endoscopy. Over 90% of patients were on additional, often multiple, prescribed medications. Our results suggest that PPIs are overprescribed in hospital practice, and there is scope to improve the quality and cost‐effectiveness of PPI prescribing.
Does the Human Motor System Simulate Pinocchio's Actions?

Corepresenting actions performed by conspecifics is essential to understanding their goals, inferring their mental states, and cooperating with them. It has recently been demonstrated that joint-action effects in a Simon task provide a good index for corepresentation. In the present study, we investigated whether corepresentation is restricted to biological agents or also occurs for nonbiological events. Participants performed a Simon task either with an image of a human hand or with a wooden analogue. The Simon-like effect emerged only when participants coacted with a biological agent. The lack of the joint-action effect when participants interacted with a wooden hand indicates that the human corepresentation system is biologically tuned.
Analysis and Simulation of Link Performance for Underwater Wireless Optical Communications

This paper investigates how to increase communication distance of underwater wireless optical communication system. In order to analyze the communication distance of UWOC, the performance of optical transmitter and receiver, underwater channel characterization and modulation schemes were discussed. The communication link model was established based the on Beer-Lambert Law. The suitable transmitter power and the maximum communication were achieved in order to achieve communication rate to 1Mbits/s and bit error rate to 10 -6 . This paper not only provides comprehensive understanding of UWOC, but also aims to provide a theoretical foundation for designing the underwater wireless optical communication system and optimizing the system parameters
Predictors of aromatase inhibitor discontinuation as a result of treatment-emergent symptoms in early-stage breast cancer.

PURPOSE Aromatase inhibitors (AIs) are effective for treatment of hormone receptor-positive breast cancer, but adherence and persistence with therapy are poor. Predictors of treatment discontinuation are not clearly defined. It is unknown whether patients with intolerable toxicity from one AI are able to tolerate another. PATIENTS AND METHODS Women with early-stage breast cancer initiating AI therapy were enrolled onto a multicenter, prospective, open-label randomized trial of exemestane versus letrozole. Patients completed symptom questionnaires at baseline and serially during therapy. Patients who developed AI-associated intolerable symptoms and discontinued treatment were given the option to switch to the other study AI after a 2- to 8-week washout period. RESULTS Of the 503 enrolled women, 32.4% discontinued initial AI therapy within 2 years because of adverse effects; 24.3% discontinued specifically because of musculoskeletal symptoms. Median time to treatment discontinuation as a result of any symptom was 6.1 months (range, 0.1 to 21.2 months) and was significantly shorter in patients randomly assigned to exemestane (hazard ratio [HR], 1.5; 95% CI, 1.1 to 2.1; P = .02). Younger age and taxane-based chemotherapy were associated with higher likelihood of treatment discontinuation (HR, 1.4; 95% CI, 1.02 to 1.9; P = .04; and HR, 1.9; 95% CI, 1.00 to 3.6; P = .048, respectively). Of the 83 patients who chose to switch to the second AI, 38.6% continued the alternate AI for a median of 13.7 months. CONCLUSION Premature discontinuation of initial AI therapy as a result of symptoms is common, although more than one third of patients may be able to tolerate a different AI medication. Additional research is needed to identify predictive tools and interventions for AI-associated treatment-emergent symptoms.
Slowly progressive aphasia without generalized dementia

Six right‐handed patients experienced a slowly progressing aphasic disorder without the additional intellectual and behavioral disturbances of dementia. The symptoms almost universally started in the presenium. The initial difficulty was an anomic aphasia in five of the patients and pure word deafness in the sixth. Continuous and gradual deterioration occurred in the five patients who presented with an anomic aphasia. They eventually experienced additional impairment of reading, writing, and comprehension. In four patients, other areas of comportment were not involved within the 5 to 11 years of follow‐up. A more generalized state of dementia may have emerged in the other two patients, but only after 7 years of progressive and debilitating aphasia. Neurodiagnostic procedures were consistent with preferential involvement of the left perisylvian region. In one patient, cortical biopsy did not show any pathognomonic change; specifically, no neurofibrillary tangles, amyloid plaques, neuronal inclusions, or gliosis were seen. This condition may constitute a syndrome of relatively focal cerebral degeneration with a predilection for the left perisylvian region.
Structured LFT representation of digital LTI filters/controllers implementation as a graphic tool

As a powerful method in modeling parametric uncertainties, the Linear Fractional Transformation (LFT) scheme in this paper is extended to digital implementation of LTI filters/controllers. The convenient coordinate transformation and modification of digital operators can be easily represented under the proposed LFT interpretation. Moreover, a specialized descriptor system is deduced from this graphic representation, and a systematic procedure of implementation is presented. An example is also provided to illustrate this approach.
Inflatable robotics for planetary applications

Space Inflatable vehicles have been finding popularity in recent years for applications as varied as spacecraft antennas, space-based telescopes, solar sails, and manned habitats [1]. Another branch of space inflatable technology has also considered developing ambient gasfilled, solar balloons for Mars as well as ambient gasfilled inflatable rovers [2]. More recently, some other intriguing space-inflatable vehicles have been proposed for the gas planets and Pluto, as well as for Saturn's moon, Titan, Neptune's moon, Triton, and Jupiter's moon, Io [3].
Articular Cartilage Contact Pressure after Tibial Tuberosity Transfer

Medial transfer of the tibial tuberosity has been commonly used for treatment of recurrent dislocation of the patella and patellofemoral malalignment. In this study, six fresh human cadaveric knees were used. Static intrajoint loads were recorded using Fuji Prescale pressure-sensitive film for contact pressure and contact area determination in a closed kinetic chain knee testing protocol. Peak pressures, average contact pressures, and contact areas of the patellofemoral and tibiofemoral joints were calculated on native intact knee specimens and after tibial tuberosity transfer. All native intact knee specimens had a normal Q angle. Medialization of the tibial tuberosity significantly increased the patellofemoral contact pressure. Medial displacement of the tibial tuberosity also significantly increased the average contact pressure of the medial tibiofemoral compartment and changed the balance of tibiofemoral joint loading. The results of our study suggest that caution should be used when transferring a patellar tendon in the face of a preexisting normal Q angle as this will result in abnormally high peak pressure within the tibiofemoral joint. Overmedialization of the tibial tuberosity should be avoided in the varus knee, the knee after medial meniscectomy, and the knee with preexisting degenerative arthritis of the medial compartment.
Multiple Mechanisms Are Involved in Inhibition of Osteoblast Differentiation by PTHrP and PTH in KS483 Cells

We examined the mechanism by which PTHrP and PTH inhibit KS483 osteoblastic differentiation. We show that PTHrP and PTH inhibit differentiation downstream of early BMP signaling and downregulated components of the hedgehog (Hh) signaling cascade. In addition, PTHrP and PTH repressed RunX2 and osx expression. Overexpression of either gene, however, could not relieve PTHrP and PTH's inhibitory actions. Our data suggest that multiple parallel mechanisms are involved in the inhibition of osteoblast differentiation and matrix mineralization by PTHrP and PTH.
Project Jagriti: Crowdsourced child abuse reporting

Child abuse and its myriad forms often go undetected due to the geographically distributed and widespread nature of the crime. The process of reporting is also long and is often used as an excuse to allow the proliferation of these activities. To prevent the crime from going unpunished, this paper introduces a browser based web application and a mobile application called Project Jagriti that uses the power of Crowdsourcing to ensure justice for the child victims. All reports of child abuse filed using the platform are forwarded to the Child Welfare Committee (CWC); a body constituted by the Government of India to oversee child welfare and expedite the process of recovery and rehabilitation of the victims. The platform also provides anonymity for encouraging users.
Coronary Interventions with Mechanical Circulatory Support

Patients in cardiogenic shock, with severely impaired left ventricular function, or intervention on the last open supplying coronary artery are summarized as so called “high risk coronary interventions” with significant risk of hemodynamic collapse and increased periprocedural mortality rate [1]. Thus, high risk percutaneous coronary interventions (PCI) may require prophylactic or standby hemodynamic mechanical circulatory support. While infusion of inotropic substances further increases myocardial oxygen consumption, other measures of support may be favourable especially in infarct related acute pump failure. However, in acute cardiogenic shock, mechanical circulatory support may depict the only option to stabilise the patient for intraor inter-hospital transfer and further diagnostic and therapeutic measures. This chapter summarises different therapeutic options of mechanical circulatory support in high risk PCI patients. Ongoing technical development as well as clinical studies may further elucidate this field of interventional cardiology in the future.
Inelastic scattering and current saturation in graphene

We present a study of transport in graphene devices on polar insulating substrates by solving the Boltzmann transport equation in the presence of graphene phonon, surface polar phonon, and Coulomb charged impurity scattering. The value of the saturated velocity shows very weak dependence on the carrier density, the nature of the insulating substrate, and the low-field mobility, varied by the charged impurity concentration. The saturated velocity of 4 10 7 –8 10 7 cm/ s calculated at room temperature is significantly larger than reported experimental values. The discrepancy is due to the self-heating effect which lowers substantially the value of the saturated velocity. We predict that by reducing the insulator oxide thickness, which limits the thermal conductance, the saturated currents can be significantly enhanced. We also calculate the surface polar phonon contribution to the low-field mobility as a function of carrier density, temperature, and distance from the substrate.
A silk-screen printed RFID tag antenna

In this work, a dipole antenna with bent ends printed on hydrophobic paper as flexible substrate using conductive ink for passive UHF RFID tags operating at 915 MHz center frequency is presented. A conductive ink prepared with silver particles was used in a silk-screen printing technique to build the conductive elements of the antenna. The technique of depositing a high viscosity conductive ink on paper through a twisted-wires screen, is a very practical method for printing any type of geometric forms, enabling fast production of patch antennas for RFID tags. Measurements were taken to verify the feasibility of using tags printed with conductive ink regarding operation, reliability of the stored information, and data exchange with the reader, via air interface. Practical results were compared with those obtained for commercial tags. For this purpose, a technique to determine the minimum power necessary to turn on passive tags is also presented. The setup includes a small, low-cost GTEM cell which is immune against electromagnetic radiation.
Characterization and autoradiographic localization of multiple tachykinin binding sites in gastrointestinal tract and bladder.

Binding sites for the [125I]Bolton-Hunter-labeled tachykinins substance K (BHSK), eledoisin (BHE) and substance P (BHSP) were investigated using crude membrane suspensions and autoradiography. In smooth muscle membranes from guinea-pig small intestine and rat duodenum, specific binding of BHSK was saturable and reversible, showing a single class of sites with a KD of 1 to 3 nM and maximum number of specific binding sites of 1 to 2 fmol/mg of wet weight tissue. Pharmacological characterization of this binding revealed a novel receptor site (K) with affinity for substance K greater than kassinin greater than or equal to eledoisin greater than neuromedin K greater than substance P greater than physalaemin. Inhibition of the binding of BHSK in membranes from mouse urinary bladder exhibited a similar K-type pattern. In rat duodenum and mouse bladder membranes, the binding of BHE was inhibited by substance K greater than kassinin greater than eledoisin greater than neuromedin K greater than substance P greater than physalaemin indicating the same receptor site as for BHSK. On the other hand, in rat cerebral cortex membranes BHE binding was inhibited by neuromedin K = kassinin = eledoisin greater than physalaemin greater than substance K greater than substance P indicating a definitive tachykinin E receptor site. The same displacement pattern of BHE binding was also detected in longitudinal muscle membranes from the guinea-pig small intestine. In mouse bladder membranes and in rat and guinea-pig intestine, the binding of BHSP was inhibited by substance P greater than physalaemin greater than substance K greater than or equal to eledoisin = kassinin greater than neuromedin K indicating a definitive tachykinin P receptor site. Autoradiographic binding sites for both BHSK and BHSP were seen in circular muscle of the rat stomach, small intestine and colon and in circular and longitudinal muscle of the guinea-pig small intestine and colon. Binding sites for BHSK, but not for BHSP, were seen in the muscularis mucosae of the gastric fundus and colon of the rat. Binding sites for BHSP, but not for BHSK, were seen in mucosa of guinea-pig colon and were densely clustered over ganglia of the myenteric and submucous plexuses in rat and guinea-pig colon. The guinea-pig intestine probably contains all three types of tachykinin binding sites whereas rat duodenum and mouse bladder contain only K and P sites. Some tissues classified previously as SP-P or SP-E may actually contain P and/or K sites. (ABSTRACT TRUNCATED AT 400 WORDS)