Miracle-dz/UniFAR-FTUs huggingface dataset:
Random sorting of Campylobacter jejuni phase variants due to a narrow bottleneck during colonization of broiler chickens

Phase variation (PV), involving stochastic switches in gene expression, is exploited by the human pathogen Campylobacter jejuni to adapt to different environmental and host niches. Phase-variable genes of C. jejuni modulate expression of multiple surface determinants, and hence may influence host colonization. Population bottlenecks can rapidly remove the diversity generated by PV, and strict single-cell bottlenecks can lead to propagation of PV states with highly divergent phenotypes. Using a combination of high-throughput fragment size analysis and comparison with in vivo and in silico bottleneck models, we have characterized a narrow population bottleneck during the experimental colonization of broiler chickens with C. jejuni strain 81-176. We identified high levels of variation in five PV genes in the inoculum, and subsequently, massively decreased population diversity following colonization. Each bird contained a dominant five-gene phasotype that was present in the inoculum indicative of random sorting through a narrow, non-selective bottleneck during colonization. These results are evidence of the potential for confounding effects of PV on in vivo studies of Campylobacter colonization factors and poultry vaccine studies. Our results are also an argument for population bottlenecks as mediators of stochastic variability in the propensity to survive through the food chain and cause clinical human disease.
Human-elephant conflict in western Thailand: Socio-economic drivers and potential mitigation strategies

Understanding human-wildlife conflict is an important first step in the conservation of highly endangered species that can have adverse effects on human communities, such as elephants. To gain insights into variables that shape attitudes toward elephant conservation in Asia, we surveyed 410 households and 46 plantation owners in seven villages around the Salakpra Wildlife Sanctuary in western Thailand, an area of high human-elephant conflict. We sought to evaluate how past experiences with elephants (positive or negative), as well as socio-economic variables (age, income level, gender, and employment type) affect attitudes toward elephant conservation and coexistence in this area. In addition, we quantified deterrence methods currently used and identify potential mitigation strategies supported by community members. In general, less supportive attitudes toward elephant conservation and coexistence were held by individuals older than 35 years of age, those who had previously had experienced negative interactions with elephants, those with lower incomes, and those working in the agricultural sector. Conversely, those who had received benefits from living near elephants (e.g., supplemental income or feelings of pride from hosting volunteers or participating in conservation work) had more supportive views of elephant coexistence. Plantation owners reported using a variety of deterrence methods with varying success, with firecrackers being the most commonly utilized method. Community members identified several potentially beneficial mitigation strategies including forest restorations and patrol teams, adding water sources to wild elephant habitat, and education of local school and community groups. Overall, our results highlight the value of community members receiving benefits from living near elephants and suggest that special incentives may be needed for demographic groups disproportionately affected by elephants (e.g. those at lower income levels, those working in agriculture). A combination of these and other approaches will be required if human-elephant coexistence in western Thailand is to be realized.
Furosine Induced Apoptosis by the Regulation of STAT1/STAT2 and UBA7/UBE2L6 Genes in HepG2 Cells

As a typical product in the Miallard reaction, research on the quantitative detection of furosine is abundant, while its bioactivities and toxic effects are still unclear. Our own work recently demonstrated the induction of furosine on apoptosis in HepG2 cells, while the related mechanism remained elusive. In this study, the effects of furosine on cell viability and apoptosis were detected to select the proper dosage, and transcriptomics detection and data analysis were performed to screen out the special genes. Additionally, SiRNA fragments of the selected genes were designed and transfected into HepG2 cells to validate the role of these genes in inducing apoptosis. Results showed that furosine inhibited cell viability and induced cell apoptosis in a dose-dependent manner, as well as activated expressions of the selected genes STAT1 (signal transducer and activator of transcription 1), STAT2 (signal transducer and activator of transcription 2), UBA7 (ubiquitin-like modifier activating enzyme 7), and UBE2L6 (ubiquitin-conjugating enzyme E2L6), which significantly affected downstream apoptosis factors Caspase-3 (cysteinyl aspartate specific proteinase-3), Bcl-2 (B-cell lymphoma gene-2), Bax (BCL2-Associated gene X), and Caspase-9 (cysteinyl aspartate specific proteinase-9). For the first time, we revealed furosine induced apoptosis through two transcriptional regulators (STAT1 and STAT2) and two ubiquitination-related enzymes (UBA7 and UBE2L6).
Vis-Eval Metric Viewer: A Visualisation Tool for Inspecting and Evaluating Metric Scores of Machine Translation Output

Machine Translation systems are usually evaluated and compared using automated evaluation metrics such as BLEU and METEOR to score the generated translations against human translations. However, the interaction with the output from the metrics is relatively limited and results are commonly a single score along with a few additional statistics. Whilst this may be enough for system comparison it does not provide much useful feedback or a means for inspecting translations and their respective scores. VisEval Metric Viewer VEMV is a tool designed to provide visualisation of multiple evaluation scores so they can be easily interpreted by a user. VEMV takes in the source, reference, and hypothesis files as parameters, and scores the hypotheses using several popular evaluation metrics simultaneously. Scores are produced at both the sentence and dataset level and results are written locally to a series of HTML files that can be viewed on a web browser. The individual scored sentences can easily be inspected using powerful search and selection functions and results can be visualised with graphical representations of the scores and distributions.
A stafétabot átadása: avagy az újabb generációk megjelenése a munkahelyeken

A generaciok temaja egyre tobbszor kerul előterbe, akar a munkahelyeken, akar a privat szfe-rakban. A generaciok mindig is kulonboztek egymastol. Azonban a kutatok felfigyeltek arra, hogy a mai kor fiatal nemzedekei tobb szempontbol is kulonboznek az idősebb nemzedekektől. A ’90-es evekben bekovetkezett tarsadalmi, szocialis es gazdasagi fordulatok kovetkezteben a fiatalok uj szokasokat, korulmenyeket, felteteleket alakitottak ki, igy az igenyeik is atalakultak. Egy HR-esnek biztositania kell a munkavallaloknak bizonyos HR funkciokat. A karriermenedzsment kezd előterbe kerulni, a munkaerő fejlesztes egyre fontosabba valik. Egy szervezetben altalaban valamennyi generacio kepviselteti magat es fontos, hogy erezzek, hogy minden generacio ertekes egy szervezetben.
SMILEE: Symmetric Multi-modal Interactions with Language-gesture Enabled (AI) Embodiment

We demonstrate an intelligent conversational agent system designed for advancing human-machine collaborative tasks. The agent is able to interpret a user’s communicative intent from both their verbal utterances and non-verbal behaviors, such as gestures. The agent is also itself able to communicate both with natural language and gestures, through its embodiment as an avatar thus facilitating natural symmetric multi-modal interactions. We demonstrate two intelligent agents with specialized skills in the Blocks World as use-cases of our system.
Camponotusfloridanus Ants Incur a Trade-Off between Phenotypic Development and Pathogen Susceptibility from Their Mutualistic Endosymbiont Blochmannia

Various insects engage in microbial mutualisms in which the reciprocal benefits exceed the costs. Ants of the genus Camponotus benefit from nutrient supplementation by their mutualistic endosymbiotic bacteria, Blochmannia, but suffer a cost in tolerating and regulating the symbiont. This cost suggests that the ants face secondary consequences such as susceptibility to pathogenic infection and transmission. In order to elucidate the symbiont’s effects on development and disease defence, Blochmannia floridanus was reduced in colonies of Camponotus floridanus using antibiotics. Colonies with reduced symbiont levels exhibited workers of smaller body size, smaller colony size, and a lower major-to-minor worker caste ratio, indicating the symbiont’s crucial role in development. Moreover, these ants had decreased cuticular melanisation, yet higher resistance to the entomopathogen Metarhizium brunneum, suggesting that the symbiont reduces the ants’ ability to fight infection, despite the availability of melanin to aid in mounting an immune response. While the benefits of improved growth and development likely drive the mutualism, the symbiont imposes a critical trade-off. The ants’ increased susceptibility to infection exacerbates the danger of pathogen transmission, a significant risk given ants’ social lifestyle. Thus, the results warrant research into potential adaptations of the ants and pathogens that remedy and exploit the described disease vulnerability.
Understanding care in the past to develop caring science of the future: a historical methodological approach

In this paper, we explore how the development of historical research methodologies during the last centuries can contribute to more diverse and interdisciplinary research in future caring science, especially towards a care focus that is more person-centred. The adding of a historical approach by professional historians to the theory of person-centredness and person-centred care can develop knowledge that enables a more holistic understanding of the patient and the development of the patient perspective from the past until today. Thus, the aim was to show how developments within historical methodology can help us to understand elements of care in the past to further develop caring science in future. Historical research methodologies have advocated a "history from below" perspective, and this has enabled the evolution of systematic approaches to historical research that can be explored and critically analysed. Linked with this, the development of a more social and cultural oriented understanding of historical research has enabled historians to explore and add knowledge from a broader societal perspective. By focusing on the life of ordinary people and taking social and cultural aspects into account when trying to reconstruct the past, we can get a deeper understanding of health, care and medical development. However, an interdisciplinary research focus on person-centredness and person-centred care that includes professional historians can be challenging. In this paper, we argue that a historical perspective is necessary to meet the challenges we face in future delivery of health care to all people, in all parts of society in an ever more global world.
B Cells and B Cell Blasts Withstand Cryopreservation While Retaining Their Functionality for Producing Antibody

In individuals who have once developed humoral immunity to an infectious/foreign antigen, the antibodies present in their body can mediate instant protection when the antigen re-enters. Such antigen-specific antibodies can be readily detected in the serum. Long term humoral immunity is, however, also critically dependent on the ability of memory B cells to engage in a secondary antibody response upon re-exposure to the antigen. Antibody molecules in the body are short lived, having a half-life of weeks, while memory B cells have a life span of decades. Therefore, the presence of serum antibodies is not always a reliable indicator of B cell memory and comprehensive monitoring of humoral immunity requires that both serum antibodies and memory B cells be assessed. The prevailing view is that resting memory B cells and B cell blasts in peripheral blood mononuclear cells (PBMC) cannot be cryopreserved without losing their antibody secreting function, and regulated high throughput immune monitoring of B cell immunity is therefore confined to—and largely limited by—the need to test freshly isolated PBMC. Using optimized protocols for freezing and thawing of PBMC, and four color ImmunoSpot® analysis for the simultaneous detection of all immunoglobulin classes/subclasses we show here that both resting memory B cells and B cell blasts retain their ability to secrete antibody after thawing, and thus demonstrate the feasibility of B cell immune monitoring using cryopreserved PBMC.
Association of higher waist circumference with milder stroke severity in acute ischaemic stroke

ABSTRACT Objectives: Although higher body mass index (BMI) was reported to be associated with increased stroke incidence, having a higher BMI is known to be associated with better clinical outcomes after stroke. However, BMI has shown conflicting associations with baseline stroke severity. The aim of our study was to assess the relationship between waist circumference (WC) at admission and baseline stroke severity among patients with ischaemic stroke. Methods: The WC of acute stroke patients was divided into sex-specific quartiles. Baseline stroke severity was categorised as mild [National Institutes of Health Stroke Scale (NIHSS) score 0–4], moderate (NIHSS score 5–10), and severe (NIHSS score ≥11). Multinomial logistic regression was used and reference categories were men or women in the lowest sex-specific WC quartiles who had experienced a mild stroke. Results: A total of 637 female and 766 male patients were included. The adjusted OR of severe stroke were 1.3 (95% CI, 0.7–2.4) for male patients in the third WC quartile, 0.7 (95% CI, 0.3–1.5) for male patients in the second WC quartile, and 0.4 (95% CI, 0.2–0.8) for male patients in the top WC quartile. The adjusted OR of severe stroke for female patients in the third WC quartile, the second WC quartile, and the top WC quartile were 0.8 (95% CI, 0.4–1.5), 0.4 (95% CI, 0.2–0.9), and 0.7 (95% CI, 0.3–1.3), respectively. Discussion: Higher WC at admission is associated with milder baseline stroke severity among patients with acute ischaemic stroke. Abbreviations: BMI: body mass index; CE: cardioembolism; CI: confidence interval; LAA: large-artery atherosclerosis; NIHSS: National Institutes of Health Stroke Scale; OR: odds ratio; SAO: small-artery occlusion; WC: waist circumference.
Undernutrition in childhood resulted in bad dietary behaviors and the increased risk of hypertension in a middle-aged Chinese population

Abstract This study was designed to explore the association between undernutrition in the growth period and cardiovascular risk factors in a middle-aged Chinese population. A total of 1756 subjects, aged 45–60 years, were invited to participate in the Hefei Nutrition and Health Study and divided into three groups according to their self-reported animal food intake in the growth period. Group 1, Group 2 and Group 3 were defined as undernutrition, nutritional improvement and the good nutrition group, respectively. In the three groups, the subjects in Groups 1 and 2 had more oil and salt intake (P<0.001), and less eggs and milk intake (P<0.001), when compared with the subjects in Group 3. After adjusting for age, education, smoking status and other confounding factors, it was found that male participants who experienced nutritional improvement before age 18 had higher risk of hypertension [odds ratio (OR)=1.68; 95% confidence intervals (CI): 1.05, 2.69] than those with good nutrition, and female participants with undernutrition (OR=1.52; 95% CI: 1.01, 2.29) and nutritional improvement (OR=1.68; 95% CI: 1.04, 2.69) before age 18 had a higher risk of hypertension than those with good nutrition. For diabetes, obesity, hypercholesterolemia and hypertriglyceridemia, our results did not found difference among the three groups both in male and female. Our findings indicated that nutritional deficiency in childhood was associated with bad dietary behaviors and a significantly increased risk of hypertension in middle age. Therefore, early adequate nutrition is very important for the prevention of non-communicable diseases later.
Gastrointestinal mucormycosis in apparently immunocompetent hosts—A review

The diagnosis of gastrointestinal (GI) mucormycosis is always a challenge due to its non‐specific clinical presentations and often diagnosed at autopsy. Recently increased number of GI mucormycosis has been reported in immunocompetent hosts and during 1948 through 2017, 200 cases of GI mucormycosis are available in literature. We could review 176 cases where case details were available. Majority (50.6%) of the cases were reported from Asia. The disease is nearly equally recorded in adults and paediatric population. The infection commonly affected the intestine (64.2%) followed by stomach (33%). A significant improvement in antemortem diagnosis was noted since 2001. Rhizopus species were the predominant (67.5%) aetiological agents. Amphotericin B was the most commonly used drug (93.4%). Despite improvement of antemortem diagnosis and therapy, the mortality was 60.5% and 67.5% in adults and children respectively. Combined medical and surgical therapy (reported in 47.8% patients) had significantly better survival rate than those receiving either of them. Analysing the clinical presentations, we propose to suspect GI mucormycosis in a malnourished/ dehydrated child (especially premature neonate) with history of exposure to broad‐spectrum antibiotics or formula/spoon feeding and presenting with mass in abdomen, abdominal distension or bilious vomiting; and an adult presenting with abdominal distension, fever or GI bleed with underlying risk factors for mucormycosis.
Language-Agnostic Relation Extraction from Abstracts in Wikis

Large-scale knowledge graphs, such as DBpedia, Wikidata, or YAGO, can be enhanced by relation extraction from text, using the data in the knowledge graph as training data, i.e., using distant supervision. While most existing approaches use language-specific methods (usually for English), we present a language-agnostic approach that exploits background knowledge from the graph instead of language-specific techniques and builds machine learning models only from language-independent features. We demonstrate the extraction of relations from Wikipedia abstracts, using the twelve largest language editions of Wikipedia. From those, we can extract 1.6 M new relations in DBpedia at a level of precision of 95%, using a RandomForest classifier trained only on language-independent features. We furthermore investigate the similarity of models for different languages and show an exemplary geographical breakdown of the information extracted. In a second series of experiments, we show how the approach can be transferred to DBkWik, a knowledge graph extracted from thousands of Wikis. We discuss the challenges and first results of extracting relations from a larger set of Wikis, using a less formalized knowledge graph.
E-HEALTH PROJECT RISK: AN EMPIRICAL STUDY

The introduction of information technology and telecommunications (ITC) in the health care sector has brought so many benefits to the health operators, managers, and patients. However, the increasing use and the application of ITC to the management and delivery of health care well known as e-health has been associated with several e-health risks that need to be examined. In this paper we point out several shortcomings of current risk conceptualization and operationalization, particularly they do not address the integration of a variety of risk components, which are crucial for capturing the essence of e-health risks. To fill this gap and drawing on risk analysis perspective we present and discuss a formal framework for e-health cloud computing project risks that captures potential scenarios, their likelihood and, the associated negative consequences. E-health risks were identified in the literature and a cluster analysis was used to classify different risks into several risk domains according to the developed e-health risk framework. Results show several domains including privacy, security, safety, liability, operational, project and business e-health risks. Implications for researchers and managers are also discussed.
Global and Widespread Local White Matter Abnormalities in Juvenile Neuronal Ceroid Lipofuscinosis

BACKGROUND AND PURPOSE: Juvenile neuronal ceroid lipofuscinosis is a progressive neurodegenerative lysosomal storage disease of childhood. It manifests with loss of vision, seizures, and loss of cognitive and motor functions leading to premature death. Previous MR imaging studies have reported cerebral and cerebellar atrophy, progressive hippocampal atrophy, thalamic signal intensity alterations, and decreased white matter volume in the corona radiata. However, conventional MR imaging findings are usually normal at younger than 10 years of age. The purpose of our study was to investigate whether diffusion MR imaging could reveal changes in white matter microstructure already present at a younger age. MATERIALS AND METHODS: We investigated global and local white matter abnormalities in 14 children with juvenile neuronal ceroid lipofuscinosis (mean age, 9.6 ± 3.4 years; 10 boys) and 14 control subjects (mean age, 11.2 ± 2.3 years; 7 boys). Twelve patients underwent follow-up MR imaging after 2 years (mean age, 11.4 ± 3.2 years; 8 boys). We performed a global analysis using 2 approaches: white matter tract skeleton and constrained spherical deconvolution–based whole-brain tractography. Then, we investigated local microstructural abnormalities using Tract-Based Spatial Statistics. RESULTS: We found globally decreased anisotropy (P = .000001) and increased diffusivity (P = .001) in patients with juvenile neuronal ceroid lipofuscinosis. In addition, we found widespread increased diffusivity and decreased anisotropy in, for example, the corona radiata (P < .001) and posterior thalamic radiation (P < .001). However, we found no differences between the first and second acquisitions. CONCLUSIONS: The patients with juvenile neuronal ceroid lipofuscinosis exhibited global and local abnormalities in white matter microstructure. Future studies could apply more specific microstructural models and study whether these abnormalities are already present at a younger age.