Miracle-dz/UniFAR-FTUs huggingface dataset:
A novel peripheral CD4+CD8+ T cell population: Inheritance of CD8α expression on CD4+ T cells

In this study we show the inheritance of a CD4+CD8+ peripheral T cell population in the H.B15 chicken strain. A large proportion of αβ T cells in peripheral blood (20–40%), spleen (10–20%) and intestinal epithelium (5–10%) co‐express CD4 and CD8α, but not CD8β. CD4+ CD8αα cells are functionally normal T cells, since they proliferate in response to mitogens and signals delivered via the αβT cell receptor as well as via the CD28 co‐receptor. These cells induce in vivo a graft versus host‐reaction, providing further evidence for their function as CD4+ T cells. The CD4+CD8αα T cell population was found in 75% of the first progeny and in 100% of further progenies, demonstrating that co‐expression of CD4 and CD8 on peripheral T cells is an inherited phenomenon. In addition, cross‐breeding data suggest a dominant Mendelian form of inheritance. The hereditary expression of CD8α on peripheral CD4+ T cells in chicken provides a unique model in which to study the regulation of CD4 and CD8 expression.
A navigation methodology of an holonomic mobile robot using optical tracking device (OTD)

The main task of a mobile robot is to perform navigation and positioning. Common mobile robot which employs line tracking system can only work on pre-constructed track where else infrared guided robot only can work within infrared's line of sight. In addition to that, the wheel driving system of the mobile robot plays a vital role in allowing the robot to navigate along a limited direction depending on its wheel architecture. To steer a robot in any direction and to know its precise location in the environment is the ultimate goal of mobile robot navigation. To achieve this, a methodology is laid out in this paper. An Optical Tracking Device (OTD) module is utilized to provide the accurate coordinates of the current location of the robot virtually on many flat surfaces. A holonomic wheel system is designed to allow the robot to navigate through multiple destinations. A navigation algorithm is developed to position the robot to any point accurately on a flat surface. This will allow the robot to freely move in any direction to reach multiple target destinations without any difficulties.
Probing the Interaction between Feline Immunodeficiency Virus and CD134 by Using the Novel Monoclonal Antibody 7D6 and the CD134 (O×40) Ligand

ABSTRACT The feline immunodeficiency virus (FIV) targets activated CD4-positive helper T cells preferentially, inducing an AIDS-like immunodeficiency in its natural host species, the domestic cat. The primary receptor for FIV is CD134, a member of the tumor necrosis factor receptor superfamily, and all primary viral strains tested to date use CD134 for infection. We examined the expression of CD134 in the cat using a novel anti-feline CD134 monoclonal antibody (MAb), 7D6, and showed that as in rats and humans, CD134 expression is restricted tightly to CD4+, and not CD8+, T cells, consistent with the selective targeting of these cells by FIV. However, FIV is also macrophage tropic, and in chronic infection the viral tropism broadens to include B cells and CD8+ T cells. Using 7D6, we revealed CD134 expression on a B220-positive (B-cell) population and on cultured macrophages but not peripheral blood monocytes. Moreover, macrophage CD134 expression and FIV infection were enhanced by activation in response to bacterial lipopolysaccharide. Consistent with CD134 expression on human and murine T cells, feline CD134 was abundant on mitogen-stimulated CD4+ T cells, with weaker expression on CD8+ T cells, concordant with the expansion of FIV into CD8+ T cells with progression of the infection. The interaction between FIV and CD134 was probed using MAb 7D6 and soluble CD134 ligand (CD134L), revealing strain-specific differences in sensitivity to both 7D6 and CD134L. Infection with isolates such as PPR and B2542 was inhibited well by both 7D6 and CD134L, suggesting a lower affinity of interaction. In contrast, GL8, CPG, and NCSU were relatively refractory to inhibition by both 7D6 and CD134L and, accordingly, may have a higher-affinity interaction with CD134, permitting infection of cells where CD134 levels are limiting.
Explaining two forms of Internet crime from two perspectives : toward stage theories for phishing and Internet scamming

Tambe Ebot, Alain Claude Explaining two forms of Internet crime from two perspectives: toward stage theories for phishing and Internet scamming Jyväskylä: University of Jyväskylä, 2017, 116 p. (Jyväskylä Studies in Computing ISSN 1456-5390; 259) ISBN 978-951-39-6953-0 (nid.) ISBN 978-951-39-6954-7 (PDF) The two studies in this dissertation examine two pervasive and common forms of Internet crimes from two different perspectives: (1) phishing from the victims’ perspective and (2) Internet scamming from the offenders’ perspective. For the former, previous phishing research is based on models that assume that fixed or static factors explain or predict people’s reasons for complying with phishing emails. These models assume that the reasons for complying with phishing emails are the same across individuals and across time. However, we argue that, whereas the act of clicking on a phishing email is the same across time for phishing victims, the reasons for complying are not the same. We address this problem with interview data from actual victims of phishing emails. The overall findings show how differences in process attributes lead individuals to comply for different reasons. We further theorize why phishing victims reside in one of two stages and propose a tailored approach to reducing phishing. For the second issue, despite making headline news and the estimated cost in the billions annually, scamming research is at an exploratory stage. Focusing on why individuals become scammers, the academic research on scamming has suggested (1) monetary rewards, (2) structural problems, and (3) affordable Internet access. We argue, however, that individuals in need of money, experiencing poverty and unemployment, and having affordable Internet access, do not become criminals. To address these problems, we interviewed actual Internet scammers. Regarding why individuals become scammers, we suggest that money is because it is needed to socialize, to enjoy an extravagant lifestyle, and to remain or become financially independent. Further, we contribute to finding how individuals become scammers by identifying three stages that respectively explain the initial progression into scamming, the specific choice of scamming and the role of the Internet, and the reasons scammers persist in scamming. We theorize these findings using criminology literature and show that neither the dispositional nor situational criminological theories can adequately explain the proclivity toward scamming and the act of practicing Internet scams. We make suggestions for practice.
Lazaroid improves intestinal blood flow in the rat during hyperdynamic bacteraemia

Background Intestinal mucosal hypoperfusion and loss of barrier function during sepsis may contribute to maintaining the septic state. Free radicals are produced during sepsis and antioxidants improve survival from experimental sepsis. It is unclear whether endothelial cell injury from free radicals results in altered microvascular reactivity. Lazaroids are antioxidants which scavenge radicals and block lipid radical chain reactions. The authors sought to determine whether lazaroids altered the intestinal microvascular responses to sepsis.
Molecular evolution of the human adult alpha-globin-like gene region: insertion and deletion of Alu family repeats and non-Alu DNA sequences.

Previous heteroduplex studies have revealed extensive sequence homology between the two human adult alpha-globin-like genes (alpha 2 and alpha 1) and their flanking regions. These homologous regions, which are interrupted by two blocks of nonhomology, each span approximately 4 kilobases [Lauer, J., Shen, C. -K. J. & Maniatis, T. (1980) Cell 20, 119-130]. We have determined 3 kilobases of DNA sequences within and flanking the nonhomologous blocks of these two tandem duplication units. A total of three Alu family repeats has been identified. Two of them are approximately 300 base pairs long and define the 3' ends of the first homology blocks. The third Alu family member is a 600-base-pair-long sequence consisting of two monomeric Alu members arranged in a head-to-tail fashion. It is located in the 3' portion of the first block of nonhomology in alpha 2-gene-containing unit. We present direct evidence that this dimeric Alu sequence was inserted at a staggered break. The second nonhomology block is the result of insertion or deletion of a 224-base-pair sequence. From these data and the calculation of sequence divergence, we propose a history for the evolution of the human adult alpha-globin-like gene region. We also suggest that DNA insertion elements may disrupt gene correction processes in the two duplication units containing alpha 2- and alpha 1-globin genes.
[Effect of Electroacupuncture at "Weiwanxiashu" (EX-B 3) on Islet Morphology and the Expression of Pancreatic Glucagon-like Peptide-1 Receptor in Type 2 Diabetes Rats].

OBJECTIVE To observe the effect of electroacupuncture (EA) on pancreatic glucagon-like peptide-1 receptor (GLP-1 R), pancreatic and duodenal homeobox 1 (PDX-1) protein expression and blood glucose in type 2 diabetes rats, so as to explore the underlying mechanism of EA treatment in improving type 2 diabetes. METHODS Sprague-Dawley rats were randomly divided into blank control group, model group, "Weiwanxiashu" (EX-B 3) group, "Xinshu" (BL 15) group, and "Shenshu" (BL 23) group, 12 rats in each group. Diabetes model was established by feeding the rat with high fat and high sugar diet combined with intraperitoneal injection of streptozotocin (35 mg/kg). All the EA groups received 2 Hz, 2 mA continuous wave treatment for 20 min everyday, 6 times per week lasting for 4 weeks. Fasting blood glucose was measured by Roche glucometer before and after treatment. Hematoxylin and eosin (HE) staining and Masson staining were used to detect pancreas morphology. GLP-1 R and PDX-1 protein expressions in the pancreas were detected by Western blot. RESULTS Compared to the blank control group, fasting blood glucose was significantly increased in the model group(P<0.01), accompanied with shrunken islet area, reduced nucleus counts of islet β cells, and compensatorily enlarged β cell nucleus. Compared to the model group, EA intervention significantly reduced fasting blood glucose level only in the EX-B 3 group (P<0.05), partly restored pancreas morphology and nucleus counts of islet β cells in the EX-B 3, BL 15, and BL 23 groups. Compared to the blank control group, GLP-1 R and PDX-1 expressions were decreased in the model group (P<0.01), while EA treatment could obviously increase GLP-1 R expression in the EX-B 3(P<0.01), BL 15 (P<0.01) and BL 23 (P<0.05) groups compared with the model group. The expression of GLP-1 R in the BL 15 group was the highest among the three EA groups (P<0.05,P<0.01), and that in the EX-B 3 group was higher than in the BL 23 group (P<0.05). There were no signifincant differences in the expression of PDX-1 protein among the three EA groups (P>0.05). CONCLUSIONS EA treatment at EX-B 3 can reduce blood glucose via regulating pancreas function, increasing pancreatic GLP-1 R expression, and partly restoring the morphology of pancreas.
Prediction of Liver Weight Recovery by an Integrated Metabolomics and Machine Learning Approach After 2/3 Partial Hepatectomy

Liver has an ability to regenerate itself in mammals, whereas the mechanism has not been fully explained. Here we used a GC/MS-based metabolomic method to profile the dynamic endogenous metabolic change in the serum of C57BL/6J mice at different times after 2/3 partial hepatectomy (PHx), and nine machine learning methods including Least Absolute Shrinkage and Selection Operator Regression (LASSO), Partial Least Squares Regression (PLS), Principal Components Regression (PCR), k-Nearest Neighbors (KNN), Support Vector Machines (SVM), Random Forest (RF), eXtreme Gradient Boosting (xgbDART), Neural Network (NNET) and Bayesian Regularized Neural Network (BRNN) were used for regression between the liver index and metabolomic data at different stages of liver regeneration. We found a tree-based random forest method that had the minimum average Mean Absolute Error (MAE), Root Mean Squared Error (RMSE) and the maximum R square (R2) and is time-saving. Furthermore, variable of importance in the project (VIP) analysis of RF method was performed and metabolites with VIP ranked top 20 were selected as the most critical metabolites contributing to the model. Ornithine, phenylalanine, 2-hydroxybutyric acid, lysine, etc. were chosen as the most important metabolites which had strong correlations with the liver index. Further pathway analysis found Arginine biosynthesis, Pantothenate and CoA biosynthesis, Galactose metabolism, Valine, leucine and isoleucine degradation were the most influenced pathways. In summary, several amino acid metabolic pathways and glucose metabolism pathway were dynamically changed during liver regeneration. The RF method showed advantages for predicting the liver index after PHx over other machine learning methods used and a metabolic clock containing four metabolites is established to predict the liver index during liver regeneration.
Translocation as a Tool for Mitigating Conflict with Leopards in Human‐Dominated Landscapes of India

Abstract: We examined the efficacy of a translocation program in which large numbers of leopards (Panthera pardus fusca) were trapped in human‐dominated landscapes where livestock attacks were common and human attacks rare and released into adjoining forested areas in an attempt to reduce leopard presence and mitigate conflicts at the capture site. In the year starting in February of 2001, 29 leopards were captured in the human‐dominated rural landscape of the Junnar region (4275 km2, 185 people/km2), Maharashtra, India, and released an average of 39.5 km away in adjoining forests. Eleven leopards were also relocated to the same forests from other districts. Prior to the large‐scale translocation program, an average of four leopard attacks on humans occurred each year between 1993 and 2001. After the translocation program was initiated, the average increased substantially to 17 attacks. Linear and logistic models showed that attack frequency increased in Junnar following nearby releases of leopards and decreased when leopards were removed for releases far away; that attacks became more lethal when the number of leopards introduced from other districts increased; and that attacks were most likely to occur in the regions where the largest number of leopards had been introduced from other areas. These results suggest that leopards did not stay at the release sites and that translocation induced attacks on people. Potential explanations for these results include increased aggression induced by stress of the translocation process, movement through unfamiliar human‐dominated landscapes following release, and loss of fear of humans due to familiarity with humans acquired during captivity. Our results show that reactive solutions to attacks on humans by leopards, such as translocation, could in fact increase human–leopard conflict. Measures to reduce human–carnivore conflicts may include more effective compensation procedures to pay livestock owners for the loss of animals to predation by carnivores, providing better methods of protection for livestock, and encouraging greater social acceptance of the presence of carnivores in human‐dominated landscapes.
A Single Domain of the Replication Termination Protein of Bacillus subtilis Is Involved in Arresting Both DnaB Helicase and RNA Polymerase*

The current models that have been proposed to explain the mechanism of replication termination are (i) passive arrest of a replication fork by the terminus (Ter) DNA-terminator protein complex that impedes the replication fork and the replicative helicase in a polar fashion and (ii) an active barrier model in which the Ter-terminator protein complex arrests a fork not only by DNA-protein interaction but also by mechanistically significant terminator protein-helicase interaction. Despite the existence of some evidence supporting in vitro interaction between the replication terminator protein (RTP) and DnaB helicase, there has been continuing debate in the literature questioning the validity of the protein-protein interaction model. The objective of the present work was two-fold: (i) to reexamine the question of RTP-DnaB interaction by additional techniques and different mutant forms of RTP, and (ii) to investigate if a common domain of RTP is involved in the arrest of both helicase and RNA polymerase. The results validate and confirm the RTP-DnaB interaction in vitro and suggest a critical role for this interaction in replication fork arrest. The results also show that the Tyr33 residue of RTP plays a critical role both in the arrest of helicase and RNA polymerase.
The Effects and Costs of a Multifactorial and Interdisciplinary Team Approach to Falls Prevention for Older Home Care Clients ‘At Risk’ for Falling: A Randomized Controlled Trial*

RÉSUMÉ Cette étude a déterminé les effets et les coûts d’une approche d’équipe multifactoriel et interdisciplinaire à la prévention des chutes. Essai contrôlé aléatoire de 109 adultes plus âgés qui sont à risque de chutes. Ce fut une stratégie de prévention multifactoriel fondée sur des données probantes de 6 mois, impliquant une équipe interdisciplinaire. Le résultat principal a été le nombre des chutes suivi pendant 6 mois. À 6 mois, il n’y a aucune différence dans le nombre moyen de chutes entre groupes. Des analyses des sous-groupes ont montrés que l’intervention réduit efficacement les chutes chez les hommes (75–84 ans) qui ont peur de tomber ou une histoire négative de chutes. Le nombre de glissades et de trébuchés a été considérablement réduit, et la santé émotionnelle a montré une amélioration plus importante dans le fonctionnement lié à la santé émotionnelle dans le groupe d’intervention. La qualité de vie a été améliorée, glissades et trébuchés ont été réduits, comme l’étaient les chutes chez les hommes qui avaient peur de tomber ou une histoire de chutes négative. ABSTRACT This study determined the effects and costs of a multifactorial, interdisciplinary team approach to falls prevention. Randomized controlled trial of 109 older adults who are at risk for falls. This was a six-month multifactorial and evidence-based prevention strategy involving an interdisciplinary team. The primary outcome was number of falls during the six-month follow-up. At six months, no difference in the mean number of falls between groups. Subgroup analyses showed that the intervention effectively reduced falls in men (75–84 years old) with a fear of falling or negative fall history. Number of slips and trips was greatly reduced; and emotional health had a greater improvement in role functioning related to emotional health in the intervention group. Quality of life was improved, slips and trips were reduced, as were falls among males (75–84 years old) with a fear of falling or negative fall history.
Visitor experience in Kafue National Park, Zambia

ABSTRACT Research is limited with respect to park-based visitor experience and satisfaction in the southern Africa region, especially in Zambia. In this study, the relationships of service quality, value, satisfaction, and future intention to revisit Kafue National Park were explored. A conceptual model was formulated and empirically tested. Data were collected from various sites with a useable sample of 336 responses. Analysis was conducted via a structural equation model to test four hypotheses. Service quality was divided into three dimensions. Staff and information were positively related to value, which in turn indirectly improved satisfaction and future intention. Accessibility to wildlife was found to be the most significant antecedent to value. Physical facilities did not significantly contribute to the strength of any factors in the model. The findings are useful for park managers to understand visitor needs and accordingly facilitate services that will optimise experience to ensure repeat visitation.
Comparison of ritonavir plus saquinavir- and nelfinavir plus saquinavir-containing regimens as salvage therapy in children with human immunodeficiency type 1 infection.

BACKGROUND In this retrospective study we compared the antiretroviral effect of regimens consisting of simultaneous administration of two protease inhibitors (PI) with at least one nucleoside reverse transcriptase inhibitor on plasma viral load (VL) and CD4 cell count in HIV-infected children intensively pretreated with nucleoside reverse transcriptase inhibitors and PIs. METHODS Eleven HIV-infected children were changed to antiretroviral combination regimens including two PIs and followed for a median time of 24 weeks. Group A comprised six patients who were given ritonavir + saquinavir (SQV) and Group B consists of five patients who were changed to nelfinavir + SQV. Patients were treated with these combinations with 2 PIs because of treatment failure (increasing viral load) of prior PI therapy or clinical signs of disease progression. OUTCOME MEASURES Serial determinations of plasma viral load (Amplicor, Roche) and CD4 cells were performed every 4 to 8 weeks. The detection limit of the Amplicor-reverse transcriptase-PCR assay was 50 copies/ml (1.7 log10). RESULTS In Group A the median VL reduction was 1.1 log10 after 3 months and 1.4 log10 after 6 months. In Group B median VL decreased 0.1 and 0.2 log10 after 3 and 6 months. In both groups during the study period none of the patients reached undetectable VL. The relative changes of CD4 cells above baseline in Group A showed a median increase of 7% after 3 months and 23% after 6 months. In Group B after 3 months CD4 cells did not increase, and after 6 months the median relative increase was only 7%. Both combination therapies were well tolerated, not necessitating any drug interruption during study period. CONCLUSIONS In children with intensive prior antiretroviral treatment, a salvage therapy including two PIs demonstrated antiretroviral efficacy in some patients. In this study the reduction of the VL as well as the increase of CD4 cells was more pronounced with ritonavir + SQV than with nelfinavir + SQV. With both combinations complete suppression of HIV replication was not achieved. Therefore the long term effect of these combinations may be limited by the emergence of resistant HIV strains.
A Meta-Analytic Review of Moral Disengagement and Cyberbullying

With the development of technology, cyberbullying prevalence rates are increasing worldwide, and a growing body of the literature has begun to document cyberbullying behavior. Moral disengagement is often considered a key correlate factor in cyberbullying. This article aims to conduct a meta-analysis review of the relationship between moral disengagement and cyberbullying and some psychosocial and cultural variables. Based on the PRISMA method, a random-effects meta-analysis is employed in this study to obtain reliable estimates of effect sizes and examine a range of moderators (age, gender, measure method, and cultural background). Relevant studies, published from 2005 to February 30, 2021, were identified through a systematic search of the Web of Science, ScienceDirect, SpringerLink, Pubmed, EBSCO, and Wiley Online Library. Finally, 38 studies (N=38,425) met the inclusion criteria. The meta-analysis conclusion demonstrated that moral disengagement positively correlated medium intensity with cyberbullying (r=0.341). Age, gender, and cultural background had moderated the relationship between moral disengagement and cyberbullying.