Miracle-dz/UniFAR-FTUs huggingface dataset:
Adenovirus-induced Reactive Astrogliosis Exacerbates the Pathology of Parkinson’s Disease

Parkinson’s disease (PD) is the most prevalent neurodegenerative motor disorder. While PD has been attributed to dopaminergic neuronal death in substantia nigra pars compacta (SNpc), accumulating lines of evidence have suggested that reactive astrogliosis is critically involved in PD pathology. These pathological changes are associated with α-synuclein aggregation, which is more prone to be induced by an A53T mutation. Therefore, the overexpression of A53T-mutated α-synuclein (A53T-α-syn) has been utilized as a popular animal model of PD. However, this animal model only shows marginal-to-moderate extents of reactive astrogliosis and astrocytic α-synuclein accumulation, while these phenomena are prominent in human PD brains. Here we show that Adeno-GFAP-GFP virus injection into SNpc causes severe reactive astrogliosis and exacerbates the A53T-α-syn-mediated PD pathology. In particular, we demonstrate that AAV-CMV-A53T-α-syn injection, when combined with Adeno-GFAP-GFP, causes more significant loss of dopaminergic neuronal tyrosine hydroxylase level and gain of astrocytic GFAP and GABA levels. Moreover, the combination of AAV-CMV-A53T-α-syn and Adeno-GFAP-GFP causes an extensive astrocytic α-syn expression, just as in human PD brains. These results are in marked contrast to previous reports that AAV-CMV-A53T-α-syn alone causes α-syn expression mostly in neurons but rarely in astrocytes. Furthermore, the combination causes a severe PD-like motor dysfunction as assessed by rotarod and cylinder tests within three weeks from the virus injection, whereas Adeno-GFAP-GFP alone or AAV-CMV-A53T-α-syn alone does not. Our findings implicate that inducing reactive astrogliosis exacerbates PD-like pathologies and propose the virus combination as an advanced strategy for developing a new animal model of PD.
Quantum Crystallography of Hydronium Cations

Five different hydronium clusters of water were extracted from the CCSD all were isomers of H! O! They were each optimized with and without the solvent environment of water. Four of the five isomers found energy minimums. All of the geometries reverted to an (H! O)(H! O)(H! O) arrangement. Some by means of single and double hydride shifts. All of the optimized geometry clusters in the gas phase came to a minimum value around -229.3383 au. The solvent optimizations had energy values around -229.4302. One structure had a geometry that was calculated to be a transition state. This transition state structure did not have its geometry morph by much except for the usual relaxing of the bonds without the crystal cage. Several levels of theory confirmed this transition state. A QTAIM study was done to ascertain the bonding mechanism of this transition state isomer. The transition state isomer had energies of -229.3128231 in the gas phase and 229.4065446 in the solvent phase. This is the first record of a water cluster being found trapped in its transition state in a crystal.
Relationship between individual alpha peak frequency and attentional performance in a multiple object tracking task among ice-hockey players

Individual alpha peak frequency (IAPF), the discrete frequency with the highest power value in the alpha oscillation range of the electroencephalogram, is a stable neurophysiological marker and is closely associated with various cognitive functions, including aspects of attention and working memory. However, the relationship between IAPF and attentional performance as well as the effects of engaging attention on IAPF are unknow. Here, we examined whether IAPF values were associated with attentional performance by evaluating accuracy during the performance of a multiple object tracking (MOT) task, a well-established paradigm for investigating goal-driven attention in dynamic environments, and whether engagement in the task affected IAPF values. In total, 18 elite players and 20 intermediate players completed the study. Resting electroencephalogram recordings were obtained for 120 s while players kept their eyes open and an additional 120 s while players’ eyes were closed, before and again after performing the MOT task. Tracking accuracy in the MOT task and IAPF values before and after the MOT task were analyzed. As expected, tracking accuracies were higher in elite players than in intermediate-level players. Baseline IAPF values were significantly and positively correlated with the accuracy of target tracking in the MOT task. IAPF values were higher in elite than intermediate players in both the eyes open and closed conditions and both before and after MOT task performance. Interindividual IAPF values did not differ before and after the MOT task. These findings indicate that IAPF is a stable marker, without intraindividual changes associated with engagement in the MOT task. Elite players had higher IAPF values and exhibited more accurate MOT performance than intermediate-level players; thus, baseline IAPF values may be useful to predict attentional performance in the MOT task among athletes.
Clinicopathologic Features of Oculopharyngodistal Myopathy With LRP12 CGG Repeat Expansions Compared With Other Oculopharyngodistal Myopathy Subtypes.

Importance Repeat expansion of CGG in LRP12 has been identified as the causative variation of oculopharyngodistal myopathy (OPDM). However, to our knowledge, the clinicopathologic features of OPDM with CGG repeat expansion in LRP12 (hereafter referred to as OPDM_LRP12) remain unknown. Objective To identify and characterize the clinicopathologic features of patients with OPDM_LRP12. Design, Setting, and Participants This case series included 208 patients with a clinical or clinicopathologic diagnosis of oculopharyngeal muscular dystrophy (OPDM) from January 1, 1978, to December 31, 2020. Patients with GCN repeat expansions in PABPN1 were excluded from the study. Repeat expansions of CGG in LRP12 were screened by repeat primed polymerase chain reaction and/or Southern blot. Main Outcomes and Measures Clinical information, muscle imaging data obtained by either computed tomography or magnetic resonance imaging, and muscle pathologic characteristics. Results Sixty-five Japanese patients with OPDM (40 men [62%]; mean [SD] age at onset, 41.0 [10.1] years) from 59 families with CGG repeat expansions in LRP12 were identified. This represents the most common OPDM subtype among all patients in Japan with genetically diagnosed OPDM. The expansions ranged from 85 to 289 repeats. A negative correlation was observed between the repeat size and the age at onset (r2 = 0.188, P = .001). The most common initial symptoms were ptosis and muscle weakness, present in 24 patients (37%). Limb muscle weakness was predominantly distal in 53 of 64 patients (83%), but 2 of 64 patients (3%) had predominantly proximal muscle weakness. Ptosis was observed in 62 of 64 patients (97%), and dysphagia or dysarthria was observed in 63 of 64 patients (98%). A total of 21 of 64 patients (33%) had asymmetric muscle weakness. Aspiration pneumonia was seen in 11 of 64 patients (17%), and 5 of 64 patients (8%) required mechanical ventilation. Seven of 64 patients (11%) developed cardiac abnormalities, and 5 of 64 patients (8%) developed neurologic abnormalities. Asymmetric muscle involvement was detected on computed tomography scans in 6 of 27 patients (22%) and on magnetic resonance imaging scans in 4 of 15 patients (27%), with the soleus and the medial head of the gastrocnemius being the worst affected. All 42 muscle biopsy samples showed rimmed vacuoles. Intranuclear tubulofilamentous inclusions were observed in only 1 of 5 patients. Conclusions and Relevance This study suggests that OPDM_LRP12 is the most frequent OPDM subtype in Japan and is characterized by oculopharyngeal weakness, distal myopathy that especially affects the soleus and gastrocnemius muscles, and rimmed vacuoles in muscle biopsy.
Prevalence of Leishmania infection in three communities of Oti Region, Ghana

Background Leishmaniasis is a neglected tropical disease caused by parasites of the genus Leishmania and is transmitted by various species of female phlebotomine sand flies. The first report of cutaneous leishmaniasis (CL) in Ghana refer to a cluster of cases in 1999–2003 in the Ho municipality of the Volta Region. We conducted an epidemiological assessment in the Oti Region, encouraged by recent reports of potential cases of CL. Methodology/Principal findings Using a cross-sectional study design, the exposure to Leishmania was investigated in three communities of the Oti Region based on the leishmanin skin test (LST). LST results for 3,071 participants comprising 1091, 848, and 1132 persons from the communities of Ashiabre, Keri, and Sibi Hilltop, indicated an overall prevalence of exposure to Leishmania infection of 41.8% and individual community prevalence of 39.4%, 55.1%, and 34.2% respectively. Being male [AOR = 1.27; CI: 1.09, 1.49], and living in Keri [AOR = 1.83; CI: 1.43, 2.34] were associated with an increase in the odds of exposure to Leishmania. Being 5–10 years old [AOR = 1.48; CI: 1.06, 2.05], 11–17 years old [AOR = 2.03; CI: 1.45, 2.85], 18–40 years old [AORR = 2.83; CI: 1.81, 4.43] and 41–65 years old [AOR = 5.08; CI: 2.98, 8.68] were also significantly associated with increased odds of being exposed to Leishmania. Conclusions/Significance This study demonstrated exposure to Leishmania in the study communities and also identified associated factors. Future efforts aimed at reducing exposure to Leishmania infection in the study area should take the associated factors into consideration.
Dampening affect via expectations: The case of ambivalence.

People often form attitudes based on a mixture of positive and negative information. This can result in mixed evaluative reactions that are associated with feeling conflicted and undecided (i.e., felt ambivalence). In the present research, we examined whether expectations of receiving mixed information could dampen felt ambivalence compared to situations where the mixed information was instead unexpected. In six experiments, expectancies of receiving mixed information-either explicitly provided or implied via patterns of behavior-led people to feel less ambivalent about a target person who had engaged in mixed social behaviors. Expectations of mixed information reduced felt ambivalence to receipt of such information in comparison to having no explicit expectancies (Studies 1-3, 5-6), negative expectancies (Study 2), positive expectancies (Study 3), and compared with expectancies formed merely on the basis of behavioral patterns (Study 4). The extent to which people felt that their expectancies of mixed information regarding the target were confirmed (vs. disconfirmed) significantly accounted for the reductions in felt ambivalence. Finally, lower felt ambivalence via manipulated expectations accounted for reduced decision time in a workplace simulation about recommending promotion or termination of an employee (Study 6). Overall, these findings bridge the extensive literatures in attitudes, social judgment, and expectations, showing that expecting mixed information can lead to reductions in felt ambivalence that have consequences for behavior. (PsycInfo Database Record (c) 2021 APA, all rights reserved).
Large-Scale Synthesis of SnO2 Nanotube Arrays as High-Performance Anode Materials of Li-Ion Batteries

This paper presents the large-scale synthesis of SnO2 nanotube arrays on titanium substrate via ZnO nanowire arrays as sacrificial templates. The SnO2 nanotube arrays on titanium substrate feature the large surface area, good electronic conductivity, and adhesion with the current collector, leading to the enhanced performance in lithium-ion batteries.
Social Support and Stressors among VA Medical Foster Home Caregivers

ABSTRACT This study aimed to identify sources of social support and stressors that facilitated or hindered caregivers in the Department of Veterans Affairs (VA) Medical Foster Home (MFH) program in performing their duties. We conducted phone interviews with 35 caregivers and found they relied on work-related, religious, and emotional social support. Caregivers’ unmet needs included a lack of time to re-charge; affordable respite services; enough VA-paid relief services, adequate relief caregivers; and ability to attend church. To address these needs, VA leadership should make MFH caregivers eligible for VA Caregiver Support Programs, encourage reciprocal caregiving arrangements, and organize virtual church services.
PalmBeat: A Kinesthetic Way to Feel Groove With Music

We propose a novel way to enhance the music experience, which evokes listeners to move to the music then feel the musicians’ groove through their body movements. In musical psychology, the groove is defined as an inner urge or a spontaneous behavior to move with music, such as hands tapping, head bobbing, and full-body dance. Although everyone feels the groove while listening to music, professional musicians perform better in catching the music rhythm and translating it to body movements. In this work, we used active force feedback to provide musicians’ hand movements for listeners and conducted a user study to evaluate the approach. Results show a significant influence on listeners’ groove perception, and the influence positively correlates with the frequency of force feedback. Compared to the condition without the force feedback, listening to the music with force feedback will enhancing listeners’ experience of groove, making them feel the groove as the musicians’ feeling.
NaviChoker: Augmenting Pressure Sensation via Pneumatic Actuator

Many technologies have been developed in recent years to present audiovisual information in new ways, but developing an information presentation interface to convey tactile information is still a challenge. We propose a tactile device using wearable technology that is an all-around pressure presentation system using pneumatic actuators. Specifically, we develop a system in which a choker equipped with a pneumatic actuator is worn around the neck, that applies pressure in any direction to indicate to the user the direction in which to walk and also when to start and stop walking. In this paper, we describe the construction of the device, evaluation experiments, our assessment of the prototype, and future plans for the device.
Augmented Percussionists: A New Musical Experience by Fusion of Melody Morphing and Electrical Muscle Stimulation

The fusion of music information processing and human physical functions will allow new musical experiences to be created. We developed an interactive music system called “Augmented Percussionists,” which provides users with the experience of playing an automatic musical performance. This system converts music arranged by melody morphing into electrical muscle stimulations (EMS) and controls the body movements of multiple people with devices attached to their hands and feet to realize the performance. The melodies used in the system are divided into segments, and each segment has multiple variations of melodies. The user can control the performers by simply by performing a melody.
Molecular mechanisms of metabotropic GABAB receptor function

This review outlines the current understanding of the structure and activation mechanism of the metabotropic GABAB receptors. Metabotropic γ-aminobutyric acid G protein–coupled receptors (GABAB) represent one of the two main types of inhibitory neurotransmitter receptors in the brain. These receptors act both pre- and postsynaptically by modulating the transmission of neuronal signals and are involved in a range of neurological diseases, from alcohol addiction to epilepsy. A series of recent cryo-EM studies revealed critical details of the activation mechanism of GABAB. Structures are now available for the receptor bound to ligands with different modes of action, including antagonists, agonists, and positive allosteric modulators, and captured in different conformational states from the inactive apo to the fully active state bound to a G protein. These discoveries provide comprehensive insights into the activation of the GABAB receptor, which not only broaden our understanding of its structure, pharmacology, and physiological effects but also will ultimately facilitate the discovery of new therapeutic drugs and neuromodulators.
Does removing default retirement ages benefit individuals? A comparative empirical case study of the university sector

In 2011, the UK government abolished the national default retirement age. While this could support extended working lives and promote individual choice, it could also be a neoliberal ‘ploy’ to individualise the risks of old age. The question, then, is what impact does the removal of mandatory retirement have in practice: does it help to promote individual choice and autonomy? Or does it lead to work intensification and the individualisation of the risks of demographic change? Or both, perhaps simultaneously? Drawing on original qualitative and quantitative empirical data from UK and USA universities, this article considers the impact of removing mandatory retirement ages on individual workers in higher education. It argues that legal reform may have prompted or encouraged work intensification in universities, including through an increased focus and use of performance management. Thus, in practice, the consequences of removing retirement ages for individuals are mixed.
Novel Electrochemical Immunoassay for Carcinoembryonic Antigen Using Ferrocenyl Polymer Nanospheres

ABSTRACT A new electrochemical immunoassay based on a sandwich format was developed for carcinoembryonic antigen using ferrocenyl polymer nanospheres as the recognition elements. Ferrocenyl nanospheres were synthesized by coordination polymerization and characterized by transmission electron microscopy, energy dispersive X-ray spectrometry, and infrared spectroscopy. Monoclonal carcinoembryonic antibodies were immobilized on a nanogold-modified gold electrode to enhance the sensitivity. Polyclonal carcinoembryonic antibodies were conjugated on ferrocenyl polymer nanospheres. Under the optimal conditions, the electrochemical signal increased with the concentration of the analyte from 0.01 to 50 ng mL−1 with a limit of detection equal to 0.004 ng mL−1. The immunosensor provided acceptable reproducibility with coefficients of variation below 10%.
APSIS: a Titan's atmosphere simulation experiment using a continuous-spectrum UV synchrotron beamline

A new reactor has been designed for experimental simulation of Titan's atmospheric photochemistry and installed at SOLEIL Synchrotron Radiation Facility whose DISCO continuous-spectrum UV beamline is used as the energy source. The products of the photochemistry are detected in situ by mass spectrometry and ex situ by the GC-MS analysis of a cryogenic trap experiment. The formation of C2H2, HCN, C2H4, C2H6, CH3CN and C2N2 is observed and confronted with results of other Titan's photochemical simulation experiments.
Learning to Predict in Networks with Heterogeneous and Dynamic Synapses

A salient difference between artificial and biological neural networks is the complexity and diversity of individual units in the latter (Tasic et al., 2018). This remarkable diversity is present in the cellular and synaptic dynamics. In this study we focus on the role in learning of one such dynamical mechanism missing from most artificial neural network models, short-term synaptic plasticity (STSP). Biological synapses have dynamics over at least two time scales: a long time scale, which maps well to synaptic changes in artificial neural networks during learning, and the short time scale of STSP, which is typically ignored. Recent studies have shown the utility of such short-term dynamics in a variety of tasks (Masse et al., 2019; Perez-Nieves et al., 2021), and networks trained with such synapses have been shown to better match recorded neuronal activity and animal behavior (Hu et al., 2020). Here, we allow the timescale of STSP in individual neurons to be learned, simultaneously with standard learning of overall synaptic weights. We study learning performance on two predictive tasks, a simple dynamical system and a more complex MNIST pixel sequence. When the number of computational units is similar to the task dimensionality, RNNs with STSP outperform standard RNN and LSTM models. A potential explanation for this improvement is the encoding of activity history in the short-term synaptic dynamics, a biological form of long short-term memory. Beyond a role for synaptic dynamics themselves, we find a reason and a role for their diversity: learned synaptic time constants become heterogeneous across training and contribute to improved prediction performance in feedforward architectures. These results demonstrate how biologically motivated neural dynamics improve performance on the fundamental task of predicting future inputs with limited computational resources, and how learning such predictions drives neural dynamics towards the diversity found in biological brains.
The measure of spatial position within groups that best predicts predation risk depends on group movement

Both empirical and theoretical studies show that an individual’s spatial position within a group can impact the risk of being targeted by predators. Spatial positions can be quantified in numerous ways, but there are no direct comparisons of different spatial measures in predicting the risk of being targeted by real predators. Here we assess these spatial measures in groups of stationary and moving virtual prey being attacked by three-spined sticklebacks (Gasterosteus aculeatus). In stationary groups, the limited domain of danger best predicted the likelihood of attack. In moving groups, the number of near neighbours was the best predictor but only over a limited range of distances within which other prey were counted. Otherwise, measures of proximity to the group’s edge outperformed measures of local crowding in moving groups. There was no evidence that predators preferentially attacked the front or back of the moving groups. Domains of danger without any limit, as originally used in the selfish herd model, were also a poor predictor of risk. These findings reveal that the collective properties of prey can influence how spatial position affects predation risk, via effects on predators’ targeting, hence selection may act differently on prey positioning behaviour depending on group movement.
The RIFF: an automated environment for studying the neural basis of auditory-guided complex behavior

Behavior consists of the interaction between an organism and its environment, and is controlled by the brain. Brain activity varies at sub-second time scales, but behavioral measures are usually coarse (often consisting of only binary trial outcomes). To overcome this mismatch, we developed the RIFF: a programmable interactive arena for freely-moving rats with multiple feeding areas, multiple sound sources, high-resolution behavioral tracking, and simultaneous electrophysiological recordings. We describe two complex tasks implemented in the RIFF. Rats quickly learned these tasks and developed anticipatory behavior. Neurons in auditory cortex and posterior insula showed sensitivity to non-auditory parameters such as location and pose. Our combination of wireless electrophysiology and detailed behavioral documentation in a controlled environment produces insights into the cognitive capabilities and learning mechanisms of rats and opens the way to a better understanding of how brains control behavior.