Miracle-dz/UniFAR-FTUs huggingface dataset:
Histochemical accumulation of oxidative damage products is associated with Alzheimer-like pathology in the canine

An important lesion in Alzheimer's disease (AD) patient brains is the neurofibrillary tangle (NFT). Hyperphosphorylated tau is its major component. In a former paper we described some NFT in the canine brain. During aging, moreover, advanced glycation end products (AGE) might accumulate. Glycated tau induces lipid peroxidation in vivo and tau and AGE antigens have been mentioned to co-localize in NFT. This indicates that AGE may play an important role in Alzheimer disease (AD) by oxidation of tau. The aim of the present study was to investigate amyloid, neurofibrillary tangles, Abeta precursor protein, Abeta, tau, ubiquitin, advanced glycation end products, 4-hyroxynonenal protein and lipofuscin in a series of dogs of varying ages. The results showed a significant positive correlation between age and amyloid quantity (Congo red staining), HNE staining and lipofuscin (LF), and between amyloid quantity and HNE staining and LF. Staining for AβPP seemed to have a tendency to increase with age, whereas staining for tau, ubiquitin and AGE each only gave limited positive results in a proportion of the older dogs. Preliminary studies including loss of cognitive capabilities in the older dogs and chemical measurement of lipofuscin-like pigment (LFP) accumulation in brain extracts revealed an increase with old age and dementia. The Congo red, HNE and LF results suggest that deposition of amyloid with aging might be associated with formation of end products of lipid peroxidation. The finding of the limited positive signals for tau, ubiquitin and AGE in some old cases might indicate that the spontaneous brain pathology of the aged dog reveals similarities to early stages observed in AD in humans especially those with Down syndrome.
Expression of stress inducible protein 1 (Stip1) in the mouse testis

Phthalate esters are considered endocrine disruptors that interfere with the endocrine balance and development of the mammalian testis. Mono‐2‐ethylhexyl phthalate (MEHP), the active metabolite of the ubiquitously used plasticizer di‐2‐ethylhexyl phthalate (DEHP), acts upon Sertoli cells as initial target. By subtractive cDNA libraries we identified genes deregulated as response to MEHP in primary cultures of mouse Sertoli cells. The expression of mouse stress inducible protein 1 (Stip1) was detected as upregulated as a result of MEHP exposure. Stip1 is a cochaperone protein that is homologous to the human heat shock cognate protein 70 (hsc70)/heat shock protein 90 (hsp90)—organizing protein (Hop). To assess the presence and localization of Stip1 in mouse testis and its potential role in stress defense, we studied the expression pattern of the Stip1 protein by immunohistochemistry and of the mRNA by in situ hybridization. Both the protein and the mRNA of Stip1 were mainly found in the cytoplasm of all types of spermatogonia and spermatocytes up till zygotene, the expression decreased during late pachytene and was very weak in diplotene spermatocytes and round spermatids. Interestingly, this expression pattern resembled the pattern of stress sensitivity of spermatogenic cells in that the most sensitive cell types show the weakest expression of Stip1. This suggests an important role for Stip1 in the ability of germ cells to survive in stress conditions including high temperatures. Mol. Reprod. Dev. 73: 1361–1366, 2006. © 2006 Wiley‐Liss, Inc.
Hela Cells Consist of Two Cell Types, as Evidenced by Cytochemical Staining for Alkaline Phosphatase Activity: A Possible Model for Cancer Stem Cell Study

Alkaline phosphatase (ALP) is known to be expressed in the several somatic stem cells and cancer cells. To investigate whether ALP may be a promising marker for cancer stem cells (CSCs), we examined the expression of ALP in human squamous cell carcinoma HeLa cells using a cytochemical
Les jeux de rôles, les jeux vidéo et le cinéma : pratiques sociales, reproblématisation de savoirs et critique

Les jeux de roles et les jeux video constituent des activites ludiques signiﬁcatives de l’epoque contemporaine, aujourd’hui associees de facon recurrente a l’industrie cinematographique. A partir de travaux empiriques, l’article se propose d’interroger le processus de creation de ces produits et d’interroger les phenomenes de reception. Ces realisations peuvent etre a l’origine d’une education informelle, dans le sens, ou, a leur contact, les jeunes mobilisent des competences pour les decoder, que les scenarios de ﬁction sur lesquels elles reposent vehiculent des contenus (se referant au social, au politique) penses en fonction des desirs et des representations supposes du “public cible”. Des lors se pose la question des capacites dont les jeunes peuvent se servir dans le cadre de la reception. L’article insiste sur le fait que celles-ci sont socialement constituees et qu’elles ne sont pas communes a l’ensemble d’une classe d’âge, a l’ensemble des “jeunes”. Tributaires du milieu social d’origine et de l’education familiale, elles dependent aussi des cultures valorisees dans les groupes de pairs au sein desquels l’individu evolue et de son niveau d’etude. Les parents des enfants qui se connectent a des salons de discussion sur internet sont de plus en plus inquiets en raison, entre autres, de nombreux reportages fortement mediatises consacres aux experiences de jeunes ﬁlles qui ont quitte le domicile familial, et se sont enfuies avec des hommes plus âges. Toutefois, il est possible de se demander si ces salons de discussion de meme que ces autres communautes virtuelles ne deviennent pas des espaces ou se developpent de nouveaux modes d’apprentissages, et ou les jeunes ont la possibilite d’explorer de nouvelles facons de communiquer, ainsi que d’exister. Cet article s’interroge sur ce que l’on peut apprendre des apprentissages que font les enfants lors de leurs conversations dans ces salons de discussion. A partir de travaux de recherche dans un centre culturel a Londres, cet article examine les rapports interactifs de quatre jeunes ﬁlles, âgees de 10 a 13 ans, pendant leurs seances de chat en ligne (habbohotel.com). L’article cherche a montrer comment celles-ci s’amusent a prendre des risques, comment elles tentent des experiences et afﬁnent le sens des mots en se lancant dans des discussions sur la vie des pre-adolescentes. Nos conclusions portent sur la maniere dont les theories sur les apprentissages informels peuvent nous aider a mieux comprendre le comportement de l’enfant face a l’ordinateur, et comment ces theories pourraient etre utiles dans le cadre de l’ecole.
Planar ultra wide band slot antenna with frequency band notch function

The development of ultra wide band (UWB) antennas for high resolution and high data transmission rates in modern communication systems is growing rapidly due to the difficult requirements of UWB antennas such as small size, non-dispersive and ultra wide-band characteristics. This paper describes a planar UWB slot antenna having frequency band notch characteristics. By inserting a V-shaped thin slot on the planar antenna, a narrow frequency band notch function has been created. Also, the notched frequency band can be varied by adjusting the length of the slot. Antenna performance including VSWR and azimuthal gain pattern of the new antenna are presented.
PHYTATE AND PHYTASE IN BROILERS-- A REVIEW

The vegetable feed ingredients used in the preparation of poultry feed contains nearly two thirds of the phosphorus as phytates. The phytates are not only indigestible /unavailable but also are known to interfere with the utilization of other nutrients. Apart from the anti-nutritional effects the phytates bind with other minerals and make them unavialble to the birds. One of the best ways to overcome the ill effects of the phytate is by the use of phytate degrading enzymes (phytase). Hence the present work was undertaken to review the antinutritive effects and by the use of phytase how to ameliorate the ill effects.
Variation in functional pelvic tilt in patients undergoing total hip arthroplasty

Aims The pelvis rotates in the sagittal plane during daily activities. These rotations have a direct effect on the functional orientation of the acetabulum. The aim of this study was to quantify changes in pelvic tilt between different functional positions. Patients and Methods Pre‐operatively, pelvic tilt was measured in 1517 patients undergoing total hip arthroplasty (THA) in three functional positions ‐ supine, standing and flexed seated (the moment when patients initiate rising from a seated position). Supine pelvic tilt was measured from CT scans, standing and flexed seated pelvic tilts were measured from standardised lateral radiographs. Anterior pelvic tilt was assigned a positive value. Results The mean pelvic tilt was 4.2° (‐20.5° to 24.5°), ‐1.3° (‐30.2° to 27.9°) and 0.6° (‐42.0° to 41.3°) in the three positions, respectively. The mean sagittal pelvic rotation from supine to standing was ‐5.5° (‐21.8° to 8.4°), from supine to flexed seated was ‐3.7° (‐48.3° to 38.6°) and from standing to flexed seated was 1.8° (‐51.8° to 39.5°). In 259 patients (17%), the extent of sagittal pelvic rotation could lead to functional malorientation of the acetabular component. Factoring in an intra‐operative delivery error of ± 5° extends this risk to 51% of patients. Conclusion Planning and measurement of the intended position of the acetabular component in the supine position may fail to predict clinically significant changes in its orientation during functional activities, as a consequence of individual pelvic kinematics. Optimal orientation is patient‐specific and requires an evaluation of functional pelvic tilt pre‐operatively.
Surgery for acute Crohn's colitis: Results and long term follow‐up

Acute Crohn's disease of the colon requiring emergency surgery is uncommon, but may be increasing in frequency. Between 1954 and 1981, 215 patients had surgery for acute inflammatory bowel disease at St. Mark's Hospital, and of these 18 had acute Crohn's colitis. There was one postoperative death, and the remaining patients were followed up for an average of 8 years. Ten patients had toxic dilatation, two a toxic dilatation with free perforation, three had perforation without dilatation and in three surgery was required for an acute deterioration. Surgical treatment included proctocolectomy (one), colectomy and ileostomy (fourteen), colectomy and ileorectal anastomosis (two) and defunctioning ileostomy alone (one). Subsequent rectal excision was necessary in ten of sixteen patients. Acute colonic Crohn's disease requiring surgery is less likely than ulcerative colitis to be amenable to restorative surgery despite a policy of rectal conservation.
Caregiver distress associated with neuropsychiatric problems in patients with early Parkinson’s disease: the Norwegian ParkWest study

Leiknes I, Tysnes O‐B, Aarsland D, Larsen JP. Caregiver distress associated with neuropsychiatric problems in patients with early Parkinson’s disease: the Norwegian ParkWest study. Acta Neurol Scand: 2010: 122: 418–424. © 2010 The Authors Journal compilation © 2010 Blackwell Munksgaard.
A testing methodology and system for functional verification of general HbbTV device

The proposed paper presents a testing methodology and system for functional verification and quality inspection of wide range of multimedia consumer devices with HbbTV functionality. The testing system comprises centralized testing control unit, system for automatic control of remote controller, transport streams generator, local web server with custom made set of web applications, automatic output data acquisition and analysis of acquired test results.
Antibacterial activity of some Indian Ayurvedic preparations against enteric bacterial pathogens

In Ayurveda, various herbal preparations are clinically used to prevent or cure infectious diseases. Herbal preparations such as Triphala churna, Hareetaki churna, Dashmula churna, Manjistadi churna, Sukhsarak churna, Ajmodadi churna, Shivkshar pachan churna, Mahasudarshan churna, Swadist Virechan churna and Pipramool churna were investigated by preparing their organic solvent extract for antibacterial potential against enteric bacterial pathogens such as Escherichia coli, Staphylococcus aureus, Enterobacter aerogenes, Pseudomonas aeruginosa, Bacillus subtilis, Klebsiella pneumoniae, Salmonella typhi, Staphylococcus epidermidis, Salmonella typhimurium and Proteus vulgaris, respectively. In the present study, Triphala churna, Hareetaki churna, Dashmula churna were potent antibacterial agents against S. epidermidis, P. vulgaris, S. aureus, E. coli, P. aeruginosa and S. typhi. The study supports the use of these herbal preparations not only as dietary supplements but also as agents to prevent or control enteric bacterial infections.
Prenatal prediction of pulmonary hypoplasia: clinical, biometric, and Doppler velocity correlates.

OBJECTIVES To determine the value of pulmonary artery Doppler velocimetry relative to fetal biometric indices and clinical correlates in the prenatal prediction of lethal lung hypoplasia (LH) in prolonged (>1 week) oligohydramnios. METHODS Forty-two singleton pregnancies with oligohydramnios associated with premature rupture of membranes ([PROM]; n = 31) or bilateral renal pathology (n = 11) were examined using color-coded Doppler ultrasound in a cross-sectional study design. Mean gestational age was 28.0 +/- 4.3 weeks (range: 20-36 weeks). Thoracic, cardiac, and abdominal circumference and the largest vertical amniotic fluid pocket were measured. Pulsed Doppler measurements of the arterial pulmonary branches were made at the level of the cardiac 4-chamber view. Diagnosis of LH was based on clinical, radiologic, and pathologic criteria. Clinicians were blinded to the prenatal measurements. RESULTS The prevalence of lethal LH was 43%. In the PROM subset, combination of onset of PROM at or =8 weeks, and degree of oligohydramnios at < or =1 cm presented the highest clinical prediction rate for lethal LH. For both the total group and the PROM subset, the highest prediction rate for lethal LH was presented by thoracic circumference/abdominal circumference ratio, peak systolic velocity in the proximal branch, and time-averaged and end-diastolic velocity in the middle branch of the pulmonary artery. In the PROM subset, the combination of all 3 clinical, biometric, and Doppler parameters revealed the most favorable combination to predict lethal LH (positive predictive value: 100%; accuracy: 93%; and sensitivity: 71%). CONCLUSION Doppler velocimetry may detect changes in pulmonary artery waveforms in the presence of LH but fails to be the ultimate test for the prenatal prediction of lethal LH. The best prediction can be achieved by combining clinical, biometric, and Doppler parameters.
Quality of life following liver transplantation in patients with liver metastases from colorectal carcinoma.

PURPOSE To study quality of life (QoL) in patients with liver metastases from colorectal carcinoma (CRC) following liver transplantation (Ltx). METHODS Ten patients participated in a prospective explorative pilot study. Inclusion criteria were nonresectable liver-only metastases determined by CT/MRI-, PET/CT- scans and colonoscopy, and ECOG 0-1. Primary outcome was QoL assessed by the EORTC-C30 questionnaires at baseline, and at 3, 6 and 12 months after Ltx. RESULTS The patients' age ranged from 50 to 63 years. Nine of 10 patients were observed for 12 months. One patient did not return the form at 6 months and died shortly after because of recurrence of the malignant disease. Compared to baseline, Ltx resulted in sustained excellent global health status scale (score of 100) in one patient, improved scores in 4 and unchanged scores in 3 patients at 12 months. The majority of the patients also reported good functional scores at follow-ups. Although two patients had marked symptoms both before and after Ltx, the patients in general reported low levels of pain and fatigue before and after surgery. CONCLUSION The present study indicates that CRC patients with liver-only metastases who receive Ltx have good QoL and have mostly minor symptoms the first year after Ltx.
Premature delivery of foetal lambs infused with glucocorticoids.

Dexamethasone caused premature delivery when infused into foetal lambs at rates of 0\m=. \06-4\m=. \0mg. /24 hr. but it had no effect when administered to pregnant ewes at the rate of 4\m=. \0mg. /24 hr. Infusions into the foetus of deoxycorticosterone or corticosterone were ineffective; mixtures of dexamethasone and deoxycorticosterone did not cause parturition more rapidly than dexamethasone alone. Thus, the ability of corticosteroids to cause premature parturition appears to depend on glucocorticoid rather than mineralocorticoid activity. Parturition induced by dexamethasone was not delayed by administration of 100 mg. progesterone/24 hr. to the ewe or to the foetus. This suggests either that withdrawal of inhibitory effects of progesterone on the myometrium can occur independently of the progesterone concentration in peripheral plasma, or that the mechanism of parturition provoked by corticosteroids in the foetus can override any regulatory influence of progesterone
An efficient MAC-signature scheme for authentication in XOR network coding

An inherent weakness of network coding is that it is vulnerable to pollute attacks from adversaries, which may jeopardize its advantages such as throughput enhancement. Message Authentication Code (MAC) is commonly used scheme to provide integrity against data pollution by appending data and shared-key dependent tags. However, this scheme results in a new type of pollution called tag pollution, where a packet with polluted tags may traffic multiple hops before detection and result in network bandwidth waste. We propose a hybrid technical scheme, called MSXOR, which can detect tag pollution with low communication and computation overhead. We address the pollution attacks by designing an efficient MAC scheme suitable for multicast XOR network coding, and tag pollution attacks by appending a signature of all the MACs. Our experimental results show that it slightly increases the computation complexity, for the sake of resisting to tag pollution.
Debe América Latina temerle a la China

(PDF disponible en Espanol en WP-536) Este articulo compara las condiciones de crecimiento economico de China y de America Latina con el fin de evaluar los temores de que la economia china desplace a la latinoamericana en las proximas decadas. Las fortalezas de China se basan en el tamano de su economia, su estabilidad macroeconomica, la abundante mano de obra barata, la rapida expansion de la infraestructura fisica y la capacidad para innovar. Las debilidades de China tienen su origen en la poca separacion que existe entre el mercado y el Estado e incluyen la debil gobernabilidad corporativa, un sistema financiero fragil y una mala asignacion del ahorro. America Latina y China comparten debilidades importantes: el imperio de la ley es debil, la corrupcion endemica y la educacion es baja y esta mal distribuida. Aunque los temores de desplazamiento de los sectores industriales y de la inversion extranjera son exagerados, China puede ser una fuente de inestabilidad financiera internacional con efectos potencialmente muy daninos para America Latina.
I. hepatitis E virus (87A strain) propagated in A549 cells

A strain of hepatitis E virus (HEV), the 87A strain isolated in 2BS cells from the feces of a patient with hepatitis E, has been reported previously. In this study, the 87A strain was propagated in A549 cells, and the marked cytopathic effect (CPE) appeared in the infected monolayer cells. The size of this virus is about 30 nm in diameter. Furthermore, HEV‐RNAfrom the supernatants of the virus of different passages was detected by polymerase chain reaction (PCR) amplification using ET1 . 1 HEV primers. A band of HEV for 239 bp from PCR products was revealed by electro‐phoresis. PCR products of the fourth passage were sequenced. These results show that the 87A virus replicates in the A549 cell line. © Wiley‐Liss, Inc.
Electrical remodeling in the failing heart

Purpose of review We focus on the molecular and cellular basis of excitability, conduction and electrical remodeling in heart failure with dyssynchronous left ventricular contraction (DHF) and its restoration by cardiac resynchronization therapy (CRT) using a canine tachy-pacing heart failure model. Recent findings The electrophysiological hallmark of cells and tissues isolated from failing hearts is prolongation of action potential duration (APD) and conduction slowing. In human studies and a number of animal models of heart failure, functional downregulation of K currents and alterations in depolarizing Na and Ca currents and transporters are demonstrated. Alterations in intercellular ion channels and extracellular matrix contribute to heterogeneity of APD and conduction slowing. The changes in cellular and tissue function are regionally heterogeneous, particularly in the DHF. Furthermore, β-adrenergic signaling and modulation of ionic currents is blunted in heart failure. CRT partially reverses the DHF-induced downregulation of K current and improves Na channel gating. CRT significantly improves Ca homeostasis, especially in lateral myocytes, and restores the DHF-induced blunted β-adrenergic receptor responsiveness. CRT abbreviates DHF-induced prolongation of APD in the lateral myocytes, reduces the left ventricular regional gradient of APD and suppresses development of early afterdepolarizations. Summary CRT partially restores DHF-induced electrophysiological remodeling, abnormal Ca homeostasis, blunted β-adrenergic responsiveness, and regional heterogeneity of APD, and thus may suppress ventricular arrhythmias and contribute to the mortality benefit of CRT as well as improving mechanical performance of the heart.
Advances in Brief Protein Kinase C Activation Increases Transepithelial Transport of Biologically Active Insulin 1

Protein kinase C activation leads to tight junctional leakiness and, consequently, to increased transepithelial (paracellular) solute flux across epithelial barriers. This leakiness is shown here to result in as much as a 20-fold increase in the transepithelial flux of insulin. Using an epithelial/ fibroblast coculture model, this transepithelially transported insulin is shown to be biologically active. The 3T3 fibroblasts situated on one side of the epithelial barrier exhibited increased insulin binding and resulting DNA synthesis when the epithelial junctions were made leaky to insulin on the opposite side of the epithelial barrier. The dramatically enhanced permeability of macromolecules across epithelial cell layers undergoing protein kinase C activation may play a significant role in epithelial cancer, immunology, and drug delivery.