Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression of mitochondrial transcription factor A (TFAM) during porcine gametogenesis and preimplantation embryo development

Mitochondrial transcription factor A (TFAM) is responsible for stability, maintenance, and transcriptional control of mitochondrial DNA (mtDNA). We have studied the expression and distribution of TFAM in the gametes and preimplantation embryos of the domestic pig (Sus scrofa). We hypothesized that TFAM is not present in the boar sperm mitochondria to reduce the possibility of paternal mtDNA propagation in the progeny. In contrast, we anticipated that Tfam gene is expressed in a developmental stage‐dependent manner in porcine oocytes and embryos. The appropriate TFAM band of 25 kDa was detected by Western blotting in ejaculated boar spermatozoa, as well as in porcine oocytes and zygotes. Boar sperm extracts also displayed several bands >25 kDa suggestive of post‐translational modification by ubiquitination, confirmed by affinity purification of ubiquitinated proteins. TFAM immunoreactivity was relegated to the sperm tail principal piece and sperm head in fully differentiated spermatozoa. The content of Tfam mRNA increased considerably from the germinal vesicle to blastocyst stage and also between in vitro fertilized and cultured blastocysts compared to in vivo‐derived blastocysts. TFAM protein accumulated in the oocytes during maturation and was reduced by proteolysis after fertilization. This pattern was not mirrored in parthenogenetically activated oocytes and zygotes reconstructed by SCNT, suggesting deviant processing of TFAM protein and transcript after oocyte/embryo manipulation. Thus, TFAM may exert a critical role in porcine gametogenesis and preimplantation embryo development. Altogether, our data on the role of TFAM in mitochondrial function and inheritance have broad implications for cell physiology and evolutionary biology. J. Cell. Physiol. 217: 529–543, 2008. © 2008 Wiley‐Liss, Inc.
320-detector row CT coronary angiography: effects of heart rate and heart rate variability on image quality, diagnostic accuracy and radiation exposure.

OBJECTIVES To evaluate the effects of heart rate and heart rate variability on image quality, patient dose and diagnostic accuracy of 320-detector row CT. METHODS 94 patients were prospectively enrolled. Heart rate was defined as the mean value of different intervals elapsing between two consecutive R waves in an electrocardiogram (R-R intervals) and the heart rate variability was calculated as the standard deviation from the average heart rate. The image quality was evaluated by four grades, according to motion artefacts ("step artefacts" and "blurring artefacts"). The diagnostic accuracy was analysed in 43 patients who were scheduled for invasive coronary angiography (ICA). The coeffects of heart rate and heart rate variability on image quality, radiation dose and diagnostic accuracy were evaluated by multivariate regression. RESULTS The mean image quality score was 1.2 ± 0.5 and the mean effective dose was 14.8 ± 9.8 mSv. The results showed that heart rate (74.0 ± 11.2 beats per minute) was the single factor influencing image quality (p<0.001) and radiation dose (p<0.001), while heart rate variability (3.7 ± 4.6) had no significant effect on them (p=0.16 and p=0.47, respectively). For 43 patients who underwent ICA, heart rate and heart rate variability showed no influence on the accuracy (p=0.17 and p=0.12, respectively). Overall sensitivity was 97.4% (37/38), specificity was 99.4% (351/353), positive predictive value was 94.9% (37/39) and negative predictive value was 99.7% (351/352). CONCLUSION 320-detector row CT, with improved longitudinal coverage of detector, resolves step artefact and high patient dose caused by irregular heart rate. However, it is still recommended to control heart rate to a lower level to eliminate blurring artefact and radiation dose.
Synthesis of a power gyrator based on sliding mode control of two cascaded boost converters using a single sliding surface

In this paper, a method to synthesize a dc power gyrator, based on cascaded connection of two dc-dc converters is introduced. The dc power gyrator is synthesized by means of a sliding-mode control that imposes a proportionality between the input current of the first stage and the output voltage of the second stage. Only one sliding surface is used to drive both the switches of the system. A systematic procedure for designing the system is presented and its stability analysis is performed analytically and validated by numerical simulations using PSIM software. As an example of application, it is shown that a gyrator based on the cascade connection of two boost converters can be a good candidate for the impedance matching between a photovoltaic (PV) generator and a resistive load.
College Students' Exposure to Tobacco Marketing in Nightclubs and Bars

Abstract Objective: To examine whether a college student's exposure to tobacco marketing in nightclubs and bars was affected by the presence of a smoke-free law. Participants: A random sample (N = 478) of students participated in the survey (no smoke-free law, n = 240; smoke-free law, n = 238). The analysis was limited to students who reported being in nightclubs and bars (n = 171). Methods: A nonexperimental, cross-sectional, 2-group design was used. Results: Students in the smoke-free law city were more likely to be approached by tobacco marketers (34.7% versus 20.2%, p = .02), offered free gifts (41.7% versus 24.2%, p = .02), and take free gifts for themselves (34.7% versus 19.2%, p = .02). They were more likely to be exposed to direct marketing strategies (1.83 versus 1.12, p = .02). There was no difference on indirect tobacco marketing by site. Conclusions: Tobacco marketing is pervasive in nightclubs and bars. Smoke-free laws may protect against exposure to secondhand smoke but not the “pro” smoking messages students encounter.
A LysM Domain-Containing Gene OsEMSA1 Involved in Embryo sac Development in Rice (Oryza sativa L.)

The embryo sac plays a vital role in sexual reproduction of angiosperms. LysM domain containing proteins with multiple lysin motifs are widespread proteins and are involved in plant defense responses against fungal chitins and bacterial peptidoglycans. Various studies have reported the role of LysM domain-containing proteins in plant defense mechanisms but their involvement in sexual reproduction remains largely unknown. Here, we report the involvement of a LysM domain-containing gene, EMBRYO SAC 1 (OsEMSA1), in the sexual reproduction of rice. The gene encoded a LysM domain-containing protein that was necessary for embryo sac development and function. The gene was expressed in root, stem, leaf tissues, panicle and ovaries and had some putative role in hormone regulation. Suppression of OsEMSA1 expression resulted in a defective embryo sac with poor differentiation of gametophytic cells, which consequently failed to attract pollen tubes and so reduced the panicle seed-setting rate. Our data offers new insight into the functions of LysM domain-containing proteins in rice.
The impact of a 3-year after-school obesity prevention program in elementary school children.

BACKGROUND Children tend to be sedentary during the after-school hours, and this has deleterious effects on their health. The objective of the present study was to determine the effects of a 3-year after-school physical activity (PA) program, without restriction of dietary energy intake, on percent body fat (%BF), cardiorespiratory fitness (CRF), and cardiometabolic markers in children. METHODS A cluster randomization design was employed. A total of 574 3rd grade children from 18 elementary schools in the southeastern United States participated. The intervention consisted of 80 minutes of age-appropriate moderate-to-vigorous PA each school day. The main outcomes of interest were %BF measured by dual-energy X-ray absorptiometry; CRF measured by heart rate in response to a submaximal step test; nonfasting total and high-density lipoprotein cholesterol (HDL-C); and resting blood pressure (BP). RESULTS Intent-to-treat analyses showed significant treatment by time interactions for %BF (p = 0.009) and CRF (p = 0.0003). The change pattern of the means suggested that %BF and CRF in intervention children improved relative to control children during the school months, rebounding to the levels of control children over the summers following years 1 and 2. Year-by-year analyses of what occurred during the months when the program was offered revealed dose–response relations for %BF and CRF, such that the clearest beneficial effects were seen for those youth who attended at least 60% of the after-school sessions. No significant intervention effects were seen for cholesterol or BP. CONCLUSIONS An after-school PA program was effective in reducing adiposity and improving CRF, especially in the children who attended the sessions at least 3 days/week. However, the favorable effects on %BF and CRF were lost over the summer. Thus, it is critical to incorporate strategies that attract and retain the children to receive an adequate dose of PA year-round.
Prediction of metabolic syndrome in women with polycystic ovary syndrome.

OBJECTIVE To identify biochemical factors that serve as predictors for the metabolic syndrome (MetS) in patients with polycystic ovary syndrome (PCOS) and to investigate the value of adipocytokines in the prediction of metabolic syndrome. MATERIAL AND METHODS A total of 91 pre-menopausal women with PCOS diagnosed according to the Rotterdam consensus criteria were recruited as study subjects. Waist circumference, blood pressure, body mass index (BMI), fasting glucose, serum lipids, insulin, FSH, LH, E2, total testosteron, homeostatic model assessment-insulin resistance (HOMA-IR), serum leptin and adiponectin levels were evaluated for all patients. RESULTS Of the 91 women with PCOS, 15 patients met the criteria for MetS. Body weight, BMI, waist circumference, systolic blood pressure, diastolic blood pressure, fasting glucose, total cholesterol, triglyceride, and VLDL concentrations were significantly higher and HDL was significantly lower in women with PCOS+MetS compared with those with PCOS only. However, the level of LDL, FSH, LH, E2 and total testesterone was not significantly different between these two groups. Women with PCOS+MetS had significantly higher levels of leptin and HOMA-IR, and significantly lower levels of adiponectin compared to the women with PCOS only. In the multiple logistic regression model, the association between HOMA-IR and leptin, and MetS remained statistically significant (p=0.001 and 0.018), while the association between adiponectin and MetS was no longer statistically significant. CONCLUSION Aside from the biochemical markers such as glucose, cholesterol and triglyceride, adipose tissue factors and insulin resistance are valuable parameters in the prediction of MetS in patients with PCOS.
Intracranial arteriovenous malformations: quantitative analysis of magnitude contrast MR angiography versus gradient-echo MR imaging versus conventional angiography.

PURPOSE To quantitatively analyze magnitude contrast (MC) magnetic resonance (MR) angiography and gradient-echo (GRE) MR imaging for evaluation of persistent transnidal blood flow in intracranial arteriovenous malformations (AVMs) of various sizes before or after stereotaxic radiosurgery. MATERIALS AND METHODS Thirty-three patients with AVMs underwent 42 MC MR angiographic (maximum intensity projection [MIP] and source imaging), GRE imaging with and without flow compensation, and conventional angiographic studies within 90 days of each other. RESULTS For MIP, source, and GRE images, the sensitivities for detection of large AVMs (> 1 cm) were 95%, 100%, and 100%, respectively, and for small AVMs (< or = 1 cm) were 27%, 50%, and 67%, respectively. MC MR angiography and GRE imaging depicted all eight nidi that were completely thrombosed on conventional angiograms. CONCLUSION GRE imaging and MC MR angiography reliably depict AVMs larger than 1 cm. GRE is more sensitive for detection of small residual nidi (< 1 cm). Source images must be evaluated to accurately assess low-flow lesions. Neither GRE nor MR angiography is reliable for detection of mean nidus diameters less than 0.36 cm.
Temporal radiance caching

Very often global illumination methods aim at simulating the light/matter interactions within static scenes. Accounting for the displacement of objects and light sources either require a complete recomputation of the global illumination solution for each frame of the animation, or involve complex data structures and algorithms for temporal optimization. Furthermore, the global illumination solutions commonly exhibit low temporal quality when used in dynamic scenes: flickering, popping, . In the context of computer-assisted effects for movies, high quality global illumination is obtained through temporal filtering: a 30 fps animation is first rendered at 60 fps by recomputing the global illumination for each frame. Then, each frame of the 30 fps animation is generated by averaging two frames of the 60 fps animation, hence reducing the temporal artifacts at the cost of high computational cost. For interactive applications such as video games, the illumination must be computed interactively. In this case, approximate models are generally preferred, such as the precomputation of a static global illumination solution, and the update of direct lighting only at runtime. This chapter describes a simple and accurate method based on temporal caching for the computation of global illumination effects in animated environments [GBP07], where viewer, objects and light sources move. This approach focuses on a temporal optimization for lighting computation based on the irradiance caching [WRC88] technique. As this algorithm leverages the spatial coherence of indirect lighting to reduce the cost of global illumination, we consider here an extension of these methods for sparse temporal sampling and interpolation. In [WRC88], Ward et al. propose a reuse an irradiance value in the neighborhood of the actual computation point. While the weighting function and gradients of [WRC88] account for the spatial change of irradiance, Temporal Radiance Caching considers the temporal change of the indirect lighting (Figure 8.2).
Determinants of Medication Adherence to Topical Glaucoma Therapy

Introduction/PurposeTo determine the associations between medical, demographic, socioeconomic, and ocular factors and adherence to topical glaucoma ocular hypotensive therapy. MethodsOne hundred sixteen patients with ocular hypertension or open-angle glaucoma from 2 tertiary glaucoma services participated in this prospective study. Adherence to ocular hypotensive therapy was measured using an electronic dose monitor (Travatan Dosing Aid, Alcon Laboratories Inc., Fort Worth, TX) and collected data at 3 months after enrollment. We used 3 different definitions of adherence: 1) Definition 1: the proportion of days taking the prescribed number of drops within 3 hours of the prescribed dosing time; 2) Definition 2: the proportion of days taking any drops within 3 hours of the prescribed dosing time; and 3) Definition 3: the proportion of days taking any drops within 6 hours of the prescribed dosing time. Univariate and multivariate models were used to determine the association between the 3 adherence definitions, medical, demographic, socioeconomic, and ocular factors at 3-month follow-up. The main outcome measures for this study were risk factors for poor objective medication adherence. ResultsAdherence, using Definition 1, Definition 2, and Definition 3, was 64%, 75%, and 80%, respectively. Age, total number of other eye diseases, and race were significantly associated with full treatment adherence (Definition 1), with race alone significantly predicting 11% of full treatment adherence. For Definition 2, age, income, level of education, and total number of eye diseases were significantly associated with partial adherence (3 h), again race alone significantly predicted 15% of partial adherence (any drops within 3 h). For Definition 3, race, income, level of education, and total number of other eye diseases significantly predicted partial adherence (any drops within 6 h), both race and income predicted 19% of partial treatment adherence. Significant differences for adherence rates between patients of European descent and those of African descent were found for all 3 definitions with those who were less adherent more likely to be of African descent. ConclusionsElectronic dose monitors provide important information regarding adherence to topical ocular hypotensive medications in glaucoma patients. Electronic dose monitors show low adherence in a significant number of participants. Future studies are needed to determine the reasons for these differences in health behaviors related to glaucoma treatment, which should guide treatment of poor adherence with glaucoma therapy.
Triplication of 16p12.1p12.3 associated with developmental and growth delay and distinctive facial features

The 16p12 region is particularly prone to genomic disorders due to the large number of low copy repeats [Martin et al., 2004; Nature 432:988–994]. We report two unrelated patients with de novo triplication of 16p12.1p12. 3 who had developmental delay and similar facial features. Patient 1 is a 4‐year‐old male with a congenital heart anomaly, bilateral cryptorchidism, chronic constipation, and developmental delay. Patient 2 is a 12‐year‐old female with prenatally diagnosed hydronephrosis, hepatobiliary disease, failure to thrive, and developmental delay. Distinctive facial features common to both patients include short palpebral fissures, bulbous nose, thin upper vermillion border, apparently lowset ears, and large ear lobes. We compare the clinical manifestations of our patients with a previously reported patient with triplication of 16p12.2. © 2015 Wiley Periodicals, Inc.
. 11 (cid:1) -Hydroxysteroid dehydrogenase and the pre-receptor regulation of corticosteroid hormone action

Two isozymes of 11 (cid:1) -hydroxysteroid dehydrogenase (11 (cid:1) -HSD1 and 11 (cid:1) -HSD2) catalyse the interconversion of hormonally active cortisol and inactive cortisone. The enzyme evolved from a metabolic pathway to a novel mechanism underpinning human disease with the elucidation of the role of the type 2 or ‘kidney’ isozyme and an inherited form of hypertension, ‘apparent mineralocorticoid excess’. ‘Cushing’s disease of the kidney’ arises because of a failure of 11 (cid:1) -HSD2 to inactivate cortisol to cortisone resulting in cortisol-induced mineralocorticoid excess.
Efficacy of Intravenous Paracetamol and Dexketoprofen on Postoperative Pain and Morphine Consumption After a Lumbar Disk Surgery

Background: We compared the analgesic effects of intravenous (IV) paracetamol with that of dexketoprofen on postoperative pain and morphine consumption during the first 24 hour after a lumbar disk surgery. Methods: This prospective, placebo-controlled, double blind study investigated the analgesic effects of IV paracetamol and dexketoprofen on postoperative pain, morphine consumption, and morphine-related side effects after a lumbar disk surgery. Sixty American Society of Anesthesiologists 1 or 2 status patients scheduled for elective lumbar disk surgery under general anesthesia were included in the study. Patients were treated using patient-controlled analgesia with morphine for 24 hours after a lumbar disk surgery and randomized to receive IV paracetamol 1 g, dexketoprofen 50 mg, or isotonic saline (placebo). The primary endpoint was pain intensity measured by the visual analogue scale, and secondary endpoints were morphine consumption and related side effects. Results: Pain intensity was lower in the dexketoprofen group (P=0.01) but not in the paracetamol group (P=0.21) when compared with the control group. Cumulative morphine consumption and morphine-related side effects did not reveal significant differences between the groups. Conclusions: The study showed that pain intensity during 24 hours after the lumbar disk surgery was significantly lowered by dexketoprofen, but not with paracetamol, as a supplemental analgesic to morphine patient-controlled analgesia when compared with controls.
Exploring the Psychological Structure of Transformational Knowledge in Visual and Haptic Intramodal Conditions Using Multidimensional Scaling

The intramodal relation between perceptual similarity and categorization performance in a psychological space, as indicated by multidimensional scaling (MDS) analysis of similarity judgments, was explored. Participants learned to classify transformed object shapes into three categories either visually or haptically via different training procedures (either random or systematic), followed by a transfer test. Learning modulated the psychological spaces, but this effect was more prevalent with haptic than with visual tasks. A prototype model for similarity ratings was illustrated in MDS space. The prototypes were multidimensionally scaled at the center of a category, rather than mirroring the bidirectional paths of their origins. Although they converged at the apex of two transformational trajectories, the category prototypes anchored at the centroid of their respective categories and became more structured as a function of learning. The reduced tendency to make errors (i.e., higher accuracy) in recognizing and classifying the category prototypes suggested that prototypical representation of a category abstracted from exemplar averaging functioned more as novel, rather than familiar, information. Findings were discussed in terms of transformational knowledge, categorical representation in three-dimensional (3D) space, and intramodal visual and haptic similarity.
A Knowledge-Based Clustering Algorithm Driven by Gene Ontology

Abstract We have developed an algorithm for inferring the degree of similarity between genes by using the graph-based structure of Gene Ontology (GO). We applied this knowledge-based similarity metric to a clique-finding algorithm for detecting sets of related genes with biological classifications. We also combined it with an expression-based distance metric to produce a co-cluster analysis, which accentuates genes with both similar expression profiles and similar biological characteristics and identifies gene clusters that are more stable and biologically meaningful. These algorithms are demonstrated in the analysis of MPRO cell differentiation time series experiments.
THz Active Imaging Systems With Real-Time Capabilities

This paper presents a survey of the status of five active THz imaging modalities which we have developed and investigated during the last few years with the goal to explore their potential for real-time imaging. We start out by introducing a novel waveguide-based all-electronic imaging system which operates at 812 GHz. Its salient feature is a 32-pixel linear detector array heterodyne-operated at the eighth subharmonic. This array in combination with a telescope optics for object distances of 2-6 m reaches a data acquisition speed suited for real-time imaging. The second system described then is again an all-electronic scanner (now for around 300 GHz ), designed for object distances of ≥ 8 m , which combines mechanical scanning in vertical direction, synthetic-aperture image generation in horizontal direction, and frequency-modulated continuous-wave sweeping for the depth information. The third and fourth systems follow an optoelectronic approach by relying on several- to multi-pixel parallel electrooptic detection. One imager is based on a pulsed THz-OPO and homodyne detection with a CCD camera, the other on either continuous-wave electronic or femtosecond optoelectronic THz sources and a photonic-mixing device (PMD) camera. The article concludes with a description of the state of the art of imaging with focal-plane arrays based on CMOS field-effect transistors.