Miracle-dz/UniFAR-FTUs huggingface dataset:
Complex and Contrast Training: Does Strength and Power Training Sequence Affect Performance-Based Adaptations in Team Sports? A Systematic Review and Meta-analysis.

Cormier, P, Freitas, TT, Rubio-Arias, JÁ, and Alcaraz, PE. Complex and contrast training: Does strength and power training sequence affect performance-based adaptations in team sports? A systematic review and meta-analysis. J Strength Cond Res XX(X): 000-000, 2020-The aims of this meta-analysis were to examine the effects of 2 different strength and power training sequences (complex: CPX; and contrast: CNT, training) on performance-based adaptations in team sports {lower-body strength (1 repetition maximum [1RM]), vertical jump (VJ), sprinting, and change of direction (COD) ability}, as well as identify factors potentially affecting said adaptations (i.e., athlete level, type of sport, intensity, and duration). CPX is the combination training that alternates biomechanically similar high load weight training exercises with lighter load power exercises, set for set (e.g., squats followed by countermovement jumps). CNT is the combination training where all high load strength exercises are performed at the beginning of the session and all lighter load power exercises at the end. After an electronic database search (PubMed, SPORTDiscus, and WoS), a total of 27 articles were included in the meta-analysis. The effects on outcomes were expressed as standardized mean differences (SMDs). Baseline to postintervention overall results for the studied variables: (a) 1RM: large effects for CPX (SMD = 2.01, 95% confidence interval [CI] 1.18-2.84) and CNT (SMD = 1.29, 95% CI 0.61-1.98); (b) VJ: large effects for CPX (SMD = 0.88, 95% CI 0.42-1.34) and medium effects for CNT (SMD = 0.55, 95% CI 0.29-0.81); (c) sprint: large effects for CPX (SMD = -0.94, 95% CI -1.33 to -0.54) and small effects for CNT (SMD = -0.27, 95% CI -0.92 to 0.39); and (d) COD: large effects for CPX (SMD = -1.17, 95% CI -1.43 to -0.90) and medium effects for CNT (SMD = -0.68, 95% CI -1.20 to -0.15). Regarding the studies that contained a control group: (a) 1RM: large effects for CPX (SMD = 1.61, 95% CI 1.12-2.10) and CNT (SMD = 1.38, 95% CI 0.30-2.46); (b) VJ: large effects for CPX (SMD = 0.85, 95% CI 0.45-1.25) and medium for CNT (SMD = 0.50, 95% CI 0.19-0.81); (c) sprint: medium effects for CPX (SMD = -0.69, 95% CI -1.02 to -0.36) and CNT (SMD = -0.51, 95% CI -0.90 to -0.11); and (d) COD: large effects for CPX (SMD = -0.83, 95% CI -1.08 to -0.59), and there were no control groups for CNT. In conclusion, both training interventions may lead to positive performance-based adaptations in team-sports with CPX interventions potentially leading to slightly greater effects.
A Review of Data Analytic Applications in Road Traffic Safety. Part 1: Descriptive and Predictive Modeling

This part of the review aims to reduce the start-up burden of data collection and descriptive analytics for statistical modeling and route optimization of risk associated with motor vehicles. From a data-driven bibliometric analysis, we show that the literature is divided into two disparate research streams: (a) predictive or explanatory models that attempt to understand and quantify crash risk based on different driving conditions, and (b) optimization techniques that focus on minimizing crash risk through route/path-selection and rest-break scheduling. Translation of research outcomes between these two streams is limited. To overcome this issue, we present publicly available high-quality data sources (different study designs, outcome variables, and predictor variables) and descriptive analytic techniques (data summarization, visualization, and dimension reduction) that can be used to achieve safer-routing and provide code to facilitate data collection/exploration by practitioners/researchers. Then, we review the statistical and machine learning models used for crash risk modeling. We show that (near) real-time crash risk is rarely considered, which might explain why the optimization models (reviewed in Part 2) have not capitalized on the research outcomes from the first stream.
MicroRNA‐361‐3p is a potent therapeutic target for oral squamous cell carcinoma

MicroRNAs (miRNAs) can act not only as tumor suppressor genes but also as oncogenes. Oncogenic miRNAs (oncomiRs) could therefore provide opportunities for the treatment of human malignancies. Here, we aimed to identify oncomiRs present in oral squamous cell carcinoma (OSCC) and addressed whether targeting these miRNAs might be useful in treatment for cancer. Functional screening for oncomiRs in a human OSCC cell line (GFP‐SAS) was carried out using the miRCURY LNA microRNA Knockdown Library – Human version 12.0. We identified a locked nucleic acid (LNA)/DNA antisense oligonucleotide against miR‐361‐3p (LNA‐miR‐361‐3p) which showed the largest degree of growth inhibition of GFP‐SAS cells. Transfection with a synthetic mimic of mature miR‐361‐3p resulted in an approximately 20% increase in the growth of GFP‐SAS cells. We identified odd‐skipped related 2 (OSR2) as a miR‐361‐3p target gene. Transfection of GFP‐SAS cells with LNA‐miR‐361‐3p caused a significant increase in the expression levels of OSR2. Cotransfection of a OSR2 3′‐UTR luciferase reporter plasmid and LNA‐miR‐361‐3p into GFP‐SAS cells produced higher levels of luciferase activity than in cells cotransfected with the LNA‐nontarget. We assessed the effect of LNA‐miR‐361‐3p on the in vivo growth of GFP‐SAS cells. We found that LNA‐miR‐361‐3p significantly reduced the size of s.c. xenografted GFP‐SAS tumors, compared to the control group treated with LNA‐NT. Finally, we observed that miR‐361‐3p is overexpressed in OSCC tissues. These results suggest that miR‐361‐3p supports the growth of human OSCC cells both in vitro and in vivo and that targeting miR‐361‐3p could be a useful therapeutic approach for patients with OSCC.
The Protagonism of the Brazilian Health Regulatory System in the Evolution of Patient Safety in the Country: History, Dilemmas, and Current Challenges

Objective This article aims to identify and analyze the legal and regulatory frameworks with an interface with patient safety, considering the historical path of the patient safety policy in Brazil. Methods This is a historical review based on the relevant literature to the topic such as papers, legislation, and official documents with an interface with public health policies from 1988 to 2019. We also performed a documentary search to include data from the Brazilian Health Regulatory Agency (ANVISA) such as normative and nonnormative regulatory instruments. After organizing the data, the process of content analysis was performed. Results We debated initially the historical aspects of sanitary surveillance of health services in addition to main actions taken by the Brazilian Health Regulatory System, which includes sanitary regulation and patient safety challenges. We identified a diversity of regulations published by ANVISA in the past decade related to patient safety, in addiction to sanitary actions. These initiatives culminated in the establishment of the National Patient Safety Program in 2013, followed by other health improvements, such as surveillance, incidents monitoring, and safe practices self-assessment. Conclusions The regulation and sanitary actions directed to patient safety in Brazil have increased after the creation of ANVISA. In the face of this activities, the social role played by the Brazilian Health Regulatory System toward the advancement in the field of risk minimization in health services can be highlighted as a protagonist in the process of promoting patient safety.
Over‐expression of RNA interference (RNAi) core machinery improves susceptibility to RNAi in silkworm larvae

RNA interference (RNAi), one of the strategies that organisms use to defend against invading viruses, is an important tool for functional genomic analysis. In insects, the efficacy of RNAi varies amongst taxa. Lepidopteran insects are, in large part, recalcitrant to RNAi. The overall goal of this study is to overcome such insensitivity in lepidopterans to RNAi. We hypothesize that over‐expression of core RNAi machinery enzymes can improve RNAi efficacy in traditionally recalcitrant species. A transgenic Bombyx mori strain, Baculovirus Immediate‐Early Gene, ie1, promoter driven expression of silkworm Dicer2 coding sequence (IE1‐BmDicer2), which over‐expresses BmDicer2, was generated by piggyBac transposon‐mediated transgenesis. Two indexes, the ratio of animals that showed a silencing phenotype and the duration of silencing, were used to evaluate silencing efficiency. Significant knockdown of target gene expression was observed at 48 h postinjection at both the transcriptional and translational levels. Furthermore, we coexpressed B. mori Argonaute 2 BmAgo2）and BmDicer 2 and found that 22% of the animals (n = 18) showed an obvious silencing effect even at 72 h, suggesting that coexpression of these two RNAi core machinery enzymes further increased the susceptibility of B. mori to injected double‐stranded RNAs. This study offers a new strategy for functional genomics research in RNAi‐refractory insect taxa in general and for lepidopterans in particular.
Validating Physiological and Biomechanical Parameters during Intermittent Swimming at Speed Corresponding to Lactate Concentration of 4 mmol·L−1

Background: Physiological and biomechanical parameters obtained during testing need validation in a training setting. The purpose of this study was to compare parameters calculated by a 5 × 200-m test with those measured during an intermittent swimming training set performed at constant speed corresponding to blood lactate concentration of 4 mmol∙L−1 (V4). Methods: Twelve competitive swimmers performed a 5 × 200-m progressively increasing speed front crawl test. Blood lactate concentration (BL) was measured after each 200 m and V4 was calculated by interpolation. Heart rate (HR), rating of perceived exertion (RPE), stroke rate (SR) and stroke length (SL) were determined during each 200 m. Subsequently, BL, HR, SR and SL corresponding to V4 were calculated. A week later, swimmers performed a 5 × 400-m training set at constant speed corresponding to V4 and BL-5×400, HR-5×400, RPE-5×400, SR-5×400, SL-5×400 were measured. Results: BL-5×400 and RPE-5×400 were similar (p > 0.05), while HR-5×400 and SR-5×400 were increased and SL-5×400 was decreased compared to values calculated by the 5 × 200-m test (p < 0.05). Conclusion: An intermittent progressively increasing speed swimming test provides physiological information with large interindividual variability. It seems that swimmers adjust their biomechanical parameters to maintain constant speed in an aerobic endurance training set of 5 × 400-m at intensity corresponding to 4 mmol∙L−1.
Leptospiral Culture without 5’-Fluorouracil Revealed Improved Leptospira Isolation from Febrile Patients in North-Eastern Malaysia

Objectives: Isolation of Leptospira by culture represents a definitive growth and confirmation of the disease, yet it is hampered with its nature of slow growth. With slight modification of culture method, the study aims to isolate and characterize Leptospira spp. from patients with acute febrile illness. Methods: A total of 109 blood samples were collected from patients with acute febrile illness that presented at the Emergency Department of Hospital Universiti Sains Malaysia, Malaysia. Clinical samples were subjected to Leptospira IgM Rapid test, microscopic agglutination test (MAT), isolation by culture method, and direct real-time PCR test. For leptospiral isolation, the samples (whole blood and deposit from spun plasma) were cultured into modified Ellinghausen McCullough Johnson Harris (EMJH) media with and without 5’-fluorouracil (5-FU). In every culture positive sample, partial 16S rRNA gene sequencing was performed for molecular identification of the isolates. Phylogenetic analysis was carried out to determine the genetic relatedness among the isolates. An inhibition of 5-FU study was performed on Leptospira interrogans serovar Canicola with different concentrations to compare the growth detection of the tested Leptospira with or without 5-FU within 7 days of incubation. Results: Leptospirosis was diagnosed in 14.7% of patients with acute febrile illness. Two Leptospira spp. (n = 2/109, 1.85%) were successfully isolated from whole blood and deposit from spun plasma samples. B004 and B208 samples were positive at day 11 and day 7, respectively, in EMJH media without addition of 5-FU. Sample B004 was identified as Leptospira interrogans and B208 as Leptospira weilli. Phylogenetic analysis confirmed that both of them were within pathogenic group and they were not related. The 5-FU inhibition study revealed that additional of 5-FU at final concentration of 200 µg/mL to EMJH media demonstrated an inhibitory effect on the growth of the tested strain Conclusion: Isolation of Leptospira spp. using EMJH media without addition of 5’-fluorouracil resulted in a better outcome. Two pathogenic Leptospira isolates were successfully cultivated from patients with acute febrile illness that were genetically not related.
Peel test to assess the adhesion strength of the dermal-epidermal junction in tissue-engineered skin.

Innovative therapies combining gene-corrected stem cells and the production of bioengineered tissues to treat epidermolysis bullosa are emerging. However, quantitative tests to measure the adhesion forces between two highly viscoelastic substrates like those found in bilayered bioengineered skin are needed and are still lacking. The objective of this study was to develop a mechanical test to measure the dermal-epidermal adhesion strength of our bilayered tissue-engineered skin substitute (TES) produced with the self-assembly method. We developed a peel test, which allows the displacement of both skin layers in a T configuration, based on the ASTM International standard. A MATLAB program was written to process and analyze raw data. The experimental set-up was tested by measuring the dermal-epidermal adhesion strength in TESs produced with normal or collagen VII-deficient cells. Our peel testing method allowed us to detect the impact of the absence of collagen VII in the dermal-epidermal adhesion strength of TESs and also to examine the progression of the dermal-epidermal adhesion strength in relation with culture time in normal TES.
Antifungal Activity of an Original Amino-Isocyanonaphthalene (ICAN) Compound Family: Promising Broad Spectrum Antifungals

Multiple drug resistant fungi pose a serious threat to human health, therefore the development of completely new antimycotics is of paramount importance. The in vitro antifungal activity of the original, 1-amino-5-isocyanonaphthalenes (ICANs) was evaluated against reference strains of clinically important Candida species. Structure-activity studies revealed that the naphthalene core and the isocyano- together with the amino moieties are all necessary to exert antifungal activity. 1,1-N-dimethylamino-5-isocyanonaphthalene (DIMICAN), the most promising candidate, was tested further in vitro against clinical isolates of Candida species, yielding a minimum inhibitory concentration (MIC) of 0.04–1.25 µg/mL. DIMICAN was found to be effective against intrinsically fluconazole resistant Candida krusei isolates, too. In vivo experiments were performed in a severly neutropenic murine model inoculated with a clinical strain of Candida albicans. Daily administration of 5 mg/kg DIMICAN intraperitoneally resulted in 80% survival even at day 13, whereas 100% of the control group died within six days. Based on these results, ICANs may become an effective clinical lead compound family against fungal pathogens.
Dogs’ Sociability, Owners’ Neuroticism and Attachment Style to Pets as Predictors of Dog Aggression

Simple Summary It is widely known that psychological characteristics, for example personality traits, can facilitate the occurrence of aggressive behavior. Using the combination of two research methods—questionnaires and behavioral testing—we investigated the associations between a dog’s personality and its aggression towards humans and animals. Due to the close relationship and co-habitation of dogs and humans, we also looked at how the owner’s personality and the dog–human emotional bond, known as attachment style, play a role in dog aggression. Our results indicated that dogs which were aggressive towards humans were less sociable, and had owners who were less emotionally stable, more distant, and less clingy and controlling, compared to non-aggressive dogs. These results emphasize the importance of owner attachment to a dog for dog behavior, and may serve as a foundation for future research on psychosocial factors influencing dog aggression. Abstract A dog’s aggressive behavior is influenced by external and internal factors, including its psychological profile. In this study, dogs’ and owners’ personalities and the owners’ attachment style to their dogs were identified and associated with owner-reported dog aggression towards humans and animals. Forty Slovenian owners participated with their dogs, of different breeds and aggression history, sorted into three groups (non-aggressive dogs, dogs aggressive towards humans, and dogs aggressive towards animals). The owners filled out three separate questionnaires that assessed dog aggression history towards different targets, owner’s personality and degree of insecure attachment styles to dogs; namely anxious and avoidant attachment. Dog personality was characterized using a standardized dog mentality assessment test, during which the dog was exposed to nine tasks, performed outside, and dogs were scored based on behaviors they exhibited. The results indicated that dogs which were aggressive towards humans were less sociable than non-aggressive dogs and this was associated with the higher neuroticism scores of their owners. We also found that dogs which were aggressive towards strangers had owners with lower scores for anxious attachment and that dogs which were aggressive towards owners had owners with higher scores for avoidant attachment. These results imply that the psychological profiles of both a dog and its owner influence dog aggression towards humans.
How flat can a horse be? Exploring 2D approximations of 3D crania in equids

Quantitative analyses of morphological variation using geometric morphometrics are often performed on 2D photos of 3D structures. It is generally assumed that the error due to the flattening of the third dimension is negligible. However, despite hundreds of 2D studies, few have actually tested this assumption and none has done it on large animals, such as those typically classified as megafauna. We explore this issue in living equids, focusing on ventral cranial variation at both micro- and macro-evolutionary levels. By comparing 2D and 3D data, we found that size is well approximated, whereas shape is more strongly impacted by 2D inaccuracies, as it is especially evident in intra-specific analyses. The 2D approximation improves when shape differences are larger, as in macroevolution, but even at this level precise inter-individual similarity relationships are altered. Despite this, main patterns of sex, species and allometric variation in 2D were the same as in 3D, thus suggesting that 2D may be a source of ‘noise’ that does not mask the main signal in the data. However, the problem is complex and any generalization premature. Morphometricians should therefore test the appropriateness of 2D using preliminary investigations in relation to the specific study questions in their own samples. We discuss whether this might be feasible using a reduced landmark configuration and smaller samples, which would save time and money. In an exploratory analysis, we found that in equids results seem robust to sampling, but become less precise and, with fewer landmarks, may slightly overestimate 2D inaccuracies.
Multidirectional Pharma-Toxicological Study on Harpagophytum procumbens DC. ex Meisn.: An IBD-Focused Investigation

In the present study, we investigated the water extract of Harpagophytum procumbens DC. ex Meisn. in an experimental model of inflammatory bowel diseases (IBDs). Additionally, a microbiological investigation was carried out to discriminate the efficacy against bacterial and fungal strains involved in IBDs. Finally, an untargeted proteomic analysis was conducted on more than one hundred colon proteins involved in tissue morphology and metabolism. The extract was effective in blunting the production of oxidative stress and inflammation, including serotonin, prostaglandins, cytokines, and transcription factors. Additionally, the extract inhibited the growth of Candida albicans and C. tropicalis. The extract was also able to exert a pro-homeostatic effect on the levels of a wide plethora of colon proteins, thus corroborating a protective effect. Conversely, the supraphysiological downregulation of cytoskeletal-related proteins involved in tissue morphology and antimicrobial barrier function suggests a warning in the use of food supplements containing H. procumbens extracts.
Pigmented Ocular Fundus Lesions Associated With Familial Adenomatous Polyposis.

A 36-year-old man was referred for evaluation after trauma to the left eye. He gave a history of familial adenomatous polyposis (FAP) diagnosed at age 15 years and treated with prophylactic colectomy. Funduscopy revealed multifocal pigmented ocular fundus lesions at the level of the retinal pigment epithelium (RPE) in both eyes (Figure 1A and 1B). The lesions were haphazardly distributed, oval-shaped, variable in size, and with irregular borders. On fundus autofluorescence, the RPE lesions were hypoautofluorescent and surrounded by trace hyperautofluorescence (Figure 1C and 1D). These findings represented the pigmented ocular fundus lesions that serve as a biomarker for FAP. [Ophthalmic Surg Lasers Imaging Retina. 2020;51:124. ].