Miracle-dz/UniFAR-FTUs huggingface dataset:
The HAUSP gene plays an important role in non‐small cell lung carcinogenesis through p53‐dependent pathways

Herpesvirus‐associated ubiquitin‐specific protease (HAUSP) directly stabilizes the tumour suppressor p53 by de‐ubiquitination. Therefore, the HAUSP gene might play an important role in carcinogenesis. In this paper, HAUSP expression and p53 gene status have been studied in relation to the expression of p53 target genes in 131 patients with non‐small cell lung cancer (NSCLC). p53 gene status was evaluated by polymerase chain reaction‐single‐strand conformation polymorphism (PCR‐SSCP) followed by sequencing. Quantitative reverse‐transcription polymerase chain reaction (RT‐PCR) was performed to evaluate the gene expression of HAUSP, p21, and bax. Immunohistochemistry was performed to evaluate the protein expression of p53, HAUSP, mdm2, p21, and bax. Fifty‐nine carcinomas (45.0%) showed reduced expression of HAUSP, and 58 carcinomas (44.3%) had mutations of p53. Concerning tumour histology, HAUSP mRNA expression was significantly lower in adenocarcinomas than in squamous cell carcinomas (p = 0.0038), while the frequency of p53 mutation was significantly higher in squamous cell carcinomas than in adenocarcinomas (p = 0.0461). There was no significant difference in HAUSP mRNA expression according to p53 gene status. In total, 93 carcinomas (71.0%) showed either mutant p53 or reduced HAUSP expression. The down‐regulation of HAUSP was associated with reduced p53 protein expression (p = 0.0593 in tumours with wild‐type p53 and p = 0.0004 in tumours with mutant p53). Furthermore, p21 and bax protein expression was significantly lower in tumours with either mutant p53 or reduced HAUSP expression than in tumours with both wild‐type p53 and positive HAUSP expression (p = 0.0440 and p = 0.0046, respectively). In addition, the simultaneous evaluation of both HAUSP expression and p53 gene status was a significant indicator of poor prognosis in adenocarcinoma patients (hazard ratio 4.840, p = 0.0357). These results suggest that reduction of HAUSP gene expression may play an important role in NSCLC carcinogenesis, especially in adenocarcinomas, through p53‐dependent pathways. Copyright © 2006 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The effects of a modern emergency medical care system in reducing automobile crash deaths.

Implementation of a modern emergency medical care system with presently available knowledge may reduce traffic mortality among those injured by 24%. Injury rates per 1,000 crashes are declining, due partially to new automobile safety features and traffic environmental changes; but the total number of traffic accidents is rising steeply, with total injuries rising at a slower rate. A study of traffic mortality in Jacksonville in 1971 offers little hope that improvements in the already highly effective Jacksonville Emergency Medical Care system can reduce traffic mortality significantly below the prevailing 1.18% of those injured, unless seat belt restraints are more widely used. Upgrading of the rural EMC systems, combined with a mandatory seat belt law, appears to offer the greatest payoff in reducing the high prevailing rural traffic death rate.
Developing a Measure of Therapist Adherence to Contingency Management: An Application of the Many-Facet Rasch Model

ABSTRACT A unique application of the Many-Facet Rasch Model (MFRM) is introduced as the preferred method for evaluating the psychometric properties of a measure of therapist adherence to Contingency Management (CM) treatment of adolescent substance use. The utility of psychometric methods based in Classical Test Theory was limited by complexities of the data, including: (1) ratings provided by multiple informants (i.e., youth, caregivers, and therapists), (2) data from separate research studies, (3) repeated measurements, (4) multiple versions of the questionnaire, and (5) missing data. Two dimensions of CM adherence were supported: adherence to Cognitive Behavioral components and adherence to monitoring components. The rating scale performed differently for items in these subscales, and of 11 items evaluated, eight were found to perform well. The MFRM is presented as a highly flexible approach that can be used to overcome the limitations of traditional methods in the development of adherence measures for evidence-based practices.
The out of phase 1 Mutant Defines a Role for PHYB in Circadian Phase Control in Arabidopsis1

Arabidopsis displays circadian rhythms in stomatal aperture, stomatal conductance, and CO2 assimilation, each of which peaks around the middle of the day. The rhythmic opening and closing of stomata confers a rhythm in sensitivity and resistance, respectively, to the toxic gas sulfur dioxide. Using this physiological assay as a basis for a mutant screen, we isolated mutants with defects in circadian timing. Here, we characterize one mutant, out of phase 1 (oop1), with the circadian phenotype of altered phase. That is, the timing of the peak (acrophase) of multiple circadian rhythms (leaf movement, CO2 assimilation, andLIGHT-HARVESTING CHLOROPHYLL a/b-BINDING PROTEINtranscription) is early with respect to wild type, although all circadian rhythms retain normal period length. This is the first such mutant to be characterized in Arabidopsis. oop1 also displays a strong photoperception defect in red light characteristic ofphytochrome B (phyB) mutants. Theoop1 mutation is a nonsense mutation ofPHYB that results in a truncated protein of 904 amino acids. The defect in circadian phasing is seen in seedlings entrained by a light-dark cycle but not in seedlings entrained by a temperature cycle. Thus, PHYB contributes light information critical for proper determination of circadian phase.
A Novel Slot Yagi-Like Multilayered Antenna With High Gain and Large Bandwidth

A novel slot Yagi-like multilayered antenna with the feasibility of obtaining high gain and large bandwidth is proposed and demonstrated at 4.2 GHz. The key design of the antenna is that patches slit with shaped slots are used as the parasitic elements to form a vertically stacked antenna structure, which is the first time that this structure has been applied to the design of Yagi-Uda antennas. The antenna is thus relatively compact, which makes it a good candidate for microwave and millimeter-wave integrated systems. Antenna prototype of this structure with two parasitic directors has been fabricated. Good agreement between the simulated and measured results has been observed. The measured results show that the antenna has achieved a wide bandwidth ( 10 dB) of 27.8% and a relatively high gain of 12.20 dB.
Genes from Pseudomonas sp. Strain BS Involved in the Conversion of l-2-Amino-Δ2-Thiazolin-4-Carbonic Acid to l-Cysteine

ABSTRACT dl-2-amino-Δ2-thiazolin-4-carbonic acid (dl-ATC) is a substrate for cysteine synthesis in some bacteria, and this bioconversion has been utilized for cysteine production in industry. We cloned a DNA fragment containing the genes involved in the conversion of l-ATC to l-cysteine from Pseudomonas sp. strain BS. The introduction of this DNA fragment into Escherichia coli cells enabled them to convert l-ATC to cysteine via N-carbamyl-l-cysteine (l-NCC) as an intermediate. The smallest recombinant plasmid, designated pTK10, contained a 2.6-kb insert DNA fragment that has l-cysteine synthetic activity. The nucleotide sequence of the insert DNA revealed that two open reading frames (ORFs) encoding proteins with molecular masses of 19.5 and 44.7 kDa were involved in the l-cysteine synthesis from dl-ATC. These ORFs were designated atcB and atcC, respectively, and their gene products were identified by overproduction of proteins encoded in each ORF and by the maxicell method. The functions of these gene products were examined using extracts of E. coli cells carrying deletion derivatives of pTK10. The results indicate that atcB and atcC are involved in the conversion of l-ATC to l-NCC and the conversion of l-NCC to cysteine, respectively. atcB was first identified as a gene encoding an enzyme that catalyzes thiazolin ring opening. AtcC is highly homologous with l-N-carbamoylases. Since both enzymes can only catalyze the l-specific conversion from l-ATC to l-NCC or l-NCC to l-cysteine, it is thought that atcB and atcC encode l-ATC hydrolase and N-carbamyl-l-cysteine amidohydrolase, respectively.
Executive functioning in offspring at risk for depression and anxiety

Background: Executive functioning deficits (EFDs) have been found in adults with major depression and some anxiety disorders, yet it is unknown whether these deficits predate onset of disorder, or whether they reflect acute symptoms. Studies of at‐risk offspring can shed light on this question by examining whether EFDs characterize children at high risk for depression and anxiety who are not yet symptomatic. Methods: This study examined neuropsychological functioning in a sample of 147 children, ages 6–17 years (M age=9.16, SD=1.82), of parents with major depression (MDD) and/or panic disorder (PD) and of controls with neither disorder. Children were assessed via structured diagnostic interviews and neuropsychological measures. Results: Although parental MDD and PD were not associated with neuropsychological impairments, presence of current offspring MDD was associated with poorer performance on several executive functioning and processing speed measures. Children with current generalized anxiety showed poorer verbal memory, whereas children with social phobia had more omissions on a continuous performance task. Conclusions: Findings suggest that EFDs do not serve as trait markers for developing anxiety or depression but appear to be symptomatic of current disorder. Depression and Anxiety, 2009. © 2009 Wiley‐Liss, Inc.
The effects of ablation diameter on the outcome of excimer laser photorefractive keratectomy. A prospective, randomized, double-blind study.

OBJECTIVE To determine the effects of the ablation diameter on the outcome of excimer laser photorefractive keratectomy. DESIGN Eighty patients were randomized to either a 5.00-mm or a 6.00-mm treatment group and within these groups underwent either a -3.00-diopter (D) or a -6.00-D correction based on their preoperative refraction. A Summit Omnimed excimer laser was used throughout the study. RESULTS In eyes treated with a 6.00-mm-diameter ablation, the initial hyperopic shift was reduced, with significant differences at 1 week with -3.00-D corrections and at 1 and 4 weeks with -6.00-D corrections (P < .01). At 6 months, the refractive changes were greater and closer to that intended with 6.00-mm-diameter ablations. The predictability of photorefractive keratectomy was significantly improved with 6.00-mm zones, with a reduction in variance of the refractive changes at all stages postoperatively (P < .05 to P < .001). With -3.00-D corrections, objective measurements showed significantly less anterior stromal haze in eyes treated with 6.00-mm zones at 6 months (P < .05). With -6.00-D corrections, haze was significantly reduced at 1, 3, and 6 months in the eyes treated with 6.00-mm zones (P < .05). Five eyes treated with 5.00-mm zones experienced severe regression of the correction, with marked corneal haze and a reduction of 3 or more lines of best corrected Snellen visual acuity at 6 months. No eyes treated with 6.00-mm zones were similarly affected. Computerized measurements of "night" halo were significantly lower in the 6.00-mm treatment groups at 1 week and at 1 and 6 months in the eyes with -3.00-D corrections and at 1 week and at 1 month in the eyes with -6.00-D corrections (P < .05). At 6 months, seven patients treated with 5.00-mm zones complained of severe disturbances of night vision. No eyes in the 6.00-mm group were similarly affected. CONCLUSIONS Treatment with 6.00-mm ablation diameters precipitated less initial overcorrection, greatly improved the predictability of photorefractive keratectomy, and was associated with a reduction in complications impairing postoperative visual performance.
Laser-Matrix-Fluoride Effects on Enamel Demineralization

Laser and fluoride have been shown to inhibit enamel demineralization. However, the role of organic matrix and their interactions remains unclear. This study investigated the interaction among CO2 laser irradiation, fluoride, and the organic matrix on the demineralization of human enamel. Twenty-four molars were selected and cut into halves. One half of each tooth was depleted of its lipid and protein. The other half served as a matched control. Each tooth half had two window areas, treated with a 2.0% NaF gel. All left windows then received a laser treatment. Next, the tooth halves were subjected to a four-day pH-cycling procedure that created caries-like lesions. Tooth sections were cut from the windows, and microradiographs were used for quantification of the demineralization. The combined fluoride-laser treatment led to 98.3% and 95.1% reductions in mineral loss for enamel with and without organic matrix, respectively, when compared with sound enamel.
Stereochemistry of Chitin Hydrolysis by a Plant Chitinase/Lysozyme and X-ray Structure of a Complex with Allosamidin

: The plant enzyme hevamine has both chitinase and lysozyme activity. HPLC analysis of the products of the hydrolysis of chitopentaose shows that hevamine acts with retention of the configuration, despite the absence of a nucleophilic or stabilizing carboxylate. To analyze the stabilization of a putative oxocarbonium ion intermediate, the X-ray structure of hevamine complexed with the inhibitor allosamidin was determined at 1.85 8, resolution. This structure supports the role of Glu127 as a proton donor. The allosamizoline group binds in the center of the active site, mimicking a reaction intermediate in which a positive charge at C1 is stabilized intramolecularly by the carbonyl oxygen of the N-acetyl group at C2. Chitin, a linear polysaccharide of p( 1,4)-linked N-acetyl-glucosamine residues, is a major constituent of the cell wall of fungi and the exoskeleton of insects and crustaceans. It can be hydrolyzed by chitinases, enzymes that are produced not only by fungi, insects, and crustaceans, for which they have an essential function during
Distribution of viruses in the Chesapeake Bay

High virus counts were found in water samples collected from the Chesapeake Bay. Viruses were enumerated by ultracentrifugation of water samples onto grids which were visualized by transmission electron microscopy. Virus counts in September 1990, April 1991, June 1991, August 1991, and October 1991 ranged between 2.6 x 10(6) and 1.4 x 10(8) viruses ml-1 with a mean of 2.5 x 10(7) viruses ml-1. Virus counts were usually at least three times higher than direct bacterial counts in corresponding samples. Virus counts in August and October were significantly higher than at the other sampling times, whereas bacterial counts were significantly lower at that time, yielding mean virus-to-bacterium ratios of 12.6 and 25.6, respectively. From analysis of morphology of the virus particles, it is concluded that a large proportion of the viruses are bacteriophages. The high virus counts obtained in this study suggest that viruses may be an important factor affecting bacterial populations in the Chesapeake Bay, with implications for gene transfer in natural aquatic bacterial populations and release of genetically engineered microorganisms to estuarine and coastal environments.
Coded-reference ultra-wideband systems

Transmitted-reference (TR) and frequency-shifted reference (FSR) ultra-wideband (UWB) systems employ pairs of reference and data signals, which are shifted in the time and frequency domains, respectively, to facilitate low-to-medium data rate communications without the need for complex channel estimation and template signal generation. On the other hand, the recently proposed coded-reference (CR) UWB systems provide orthogonalization of the reference and data signals in the code domain, which has advantages in terms of performance and/or implementation complexity. In this paper, CR UWB systems are investigated. First, it is shown that a CR UWB system can be considered as a generalized non-coherent pulse-position modulated system. Then, an optimal receiver according to the Bayes decision rule is derived for CR UWB systems. In addition, the asymptotic optimality properties of the conventional CR UWB receivers are investigated. Finally, simulation results are presented to compare the performance of the optimal and conventional CR UWB receivers.
Dynamic clustering scheme for distributed spectrum sensing in cognitive radio networks

The detection performance of cooperative spectrum sensing in cognitive radio networks is compromised by shadowing, multi-path fading and receiver uncertainty problems when the sensing results are forwarded to the fusion centre. In this paper, we propose a dynamic clustering scheme for a two-level hierarchical cognitive radio network to improve the sensing efficiency. By dynamic optimal clustering of all the cognitive radio users and selection of the user with highest instantaneous channel gain and highest detection probability for making cluster decision, our proposed model exploits spatial diversity and improves local sensing accuracy. Further, our scheme also exploits user selection diversity by forwarding results to the fusion centre using channel with highest instantaneous SNR. Moreover, by dynamic switching in the fusion centre along with forwarding of the censored results, our scheme achieves increased energy efficiency and reduced control channel bandwidth. Furthermore, consideration of fading channels within clusters, makes our scheme more generic than existing works. Performance evaluations evaluate and validate the improved spectrum sensing accuracy and energy efficiency of our proposed scheme compared to the conventional spectrum sensing methods.
Gait Recognition of Lower Limb Rehabilitation Robot Based on Support Vector Machine

With the aging of population becoming serious, the number of elderly have to ask others for help in their daily activities. It causes an incubus to the family and society. We help these people to improve their self-care ability. In this paper, The Kinect sensor is installed in the front of the lower limb rehabilitation robot. The horizontal distances are acquired from each marks and the length of the Kinect plane in the process of rehabilitation training. Then we used the support vector classifier to identify the different people. Targeted rehabilitation training which is more effective for everyone.
Toenail Metal Concentration as a Biomarker of Occupational Welding Fume Exposure

In populations exposed to heavy metals, there are few biomarkers that capture intermediate exposure windows. We sought to determine the correlation between toenail metal concentrations and prior 12-month work activity in welders with variable, metal-rich, welding fume exposures. Forty-eight participants, recruited through a local union, provided 69 sets of toenail clippings. Union-supplied and worker-verified personal work histories were used to quantify hours welded and respirator use. Toenail samples were digested and analyzed for lead (Pb), manganese (Mn), cadmium (Cd), nickel (Ni), and arsenic (As) using ICP-MS. Spearman correlation coefficients were used to examine the correlation between toenail metal concentrations. Using mixed models to account for multiple participation times, we divided hours welded into three-month intervals and examined how weld hours correlated with log-transformed toenail Pb, Mn, Cd, Ni, and As concentrations. Highest concentrations were found for Ni, followed by Mn, Pb and As, and Cd. All the metals were significantly correlated with one another (rho range = 0.28–0.51), with the exception of Ni and As (rho = 0.20, p = 0.17). Using mixed models adjusted for age, respirator use, smoking status, and BMI, we found that Mn was associated with weld hours 7–9 months prior to clipping (p = 0.003), Pb was associated with weld hours 10–12 months prior to clipping (p = 0.03) and over the entire year (p = 0.04). Cd was associated with weld hours 10–12 months prior to clipping (p = 0.05), and also with the previous year's total hours welded (p = 0.02). The association between Ni and weld hours 7–9 months prior to clipping approached significance (p = 0.06). Toenail metal concentrations were not associated with the long-term exposure metric, years as a welder. Results suggest Mn, Pb, and Cd may have particular windows of relevant exposure that reflect work activity. In a population with variable exposure, toenails may serve as useful biomarkers for occupational metal fume exposures to Mn, Pb, and Cd during distinct periods over the year prior to sample collection.
Dynamic Compact Thermal Model for stacked-die components

The present work proposes an approach to generate Dynamic Compact Thermal Models or “DCTMs” dedicated to electronic components. This one is based on the European project DELPHI, which defined the first comprehensive methodology concerning the generation of thermal behavioral model, Boundary Condition Independent, called Compact Thermal Models or “CTMs”. Unfortunately, the scope of “CTMs” was limited to the steady state as well as for single chip packages. But, the latest trend toward higher and higher density packaging using several chips requires henceforth a methodology capable to take into account the transient regime for 3D integration technologies like stacked-die solution. Following the CTM's modus operandi the DCTMs were conceived to propose a RC network able to predict a set of sensitive component temperatures with a minimized difference during component duty cycle. This work suggests the use of the genetic algorithms fitting technique that turns out relevant for the realization of DCTM, as well as the conventional DELPHI CTM.
Validity of Daily and Weekly Self-Reported Training Load Measures in Adolescent Athletes

Abstract Phibbs, PJ, Roe, G, Jones, B, Read, DB, Weakley, J, Darrall-Jones, J, and Till, K. Validity of daily and weekly self-reported training load measures in adolescent athletes. J Strength Cond Res 31(4): 1121–1126, 2017—The primary aim of the study was to assess the level of agreement between the criterion session rating of perceived exertion (sRPE30min) and a practical measure of a self-reported Web-based training load questionnaire 24 hours after training (sRPE24h) in adolescent athletes. The secondary aim was to assess the agreement between weekly summated sRPE24h values (∑sRPE24h) and a weekly Web-based training diary (sRPEweekly) for all field-based training accumulated on a subsequent training week. Thirty-six male adolescent rugby players (age, 16.7 ± 0.5 years) were recruited from a regional academy. Measures of sRPE30min were recorded 30 minutes after a typical field-based training session. Participants then completed the sRPE24h via a Web-based training load questionnaire 24 hours after training, reporting both session duration and intensity. In addition, on a subsequent week, participants completed the sRPE24h daily and then completed the sRPEweekly at the end of the week, using the same Web-based platform, to recall all field-based training session durations and intensities over those 7 days. Biases were trivial between sRPE30min and sRPE24h for sRPE (0.3% [−0.9 to 1.5]), with nearly perfect correlations (0.99 [0.98–0.99]) and small typical error of the estimate (TEE; 4.3% [3.6–5.4]). Biases were trivial between ∑sRPE24h and sRPEweekly for sRPE (5.9% [−2.1 to 14.2]), with very large correlations (0.87 [0.78–0.93]) and moderate TEE of 28.5% [23.3–36.9]. The results of this study show that sRPE24h is a valid and robust method to quantify training loads in adolescent athletes. However, sRPEweekly was found to have a substantial TEE (28.5%), limiting practical application.