Miracle-dz/UniFAR-FTUs huggingface dataset:
H2‐Free Re‐Based Catalytic Dehydroxylation of Aldaric Acid to Muconic and Adipic Acid Esters

Abstract As one of the most demanded dicarboxylic acids, adipic acid can be directly produced from renewable sources. Hexoses from (hemi)cellulose are oxidized to aldaric acids and subsequently catalytically dehydroxylated. Hitherto performed homogeneously, we present the first heterogeneous catalytic process for converting an aldaric acid into muconic and adipic acid. The contribution of leached Re from the solid pre‐reduced catalyst was also investigated with hot‐filtration test and found to be inactive for dehydroxylation. Corrosive or hazardous (HBr/H2) reagents are avoided and simple alcohols and solid Re/C catalysts in an inert atmosphere are used. At 120 °C, the carboxylic groups are protected by esterification, which prevents lactonization in the absence of water or acidic sites. Dehydroxylation and partial hydrogenation yield monohexenoates (93 %). For complete hydrogenation to adipate, a 16 % higher activation barrier necessitates higher temperatures.
Occurrence of Brucella ceti in striped dolphins from Italian Seas

Brucella ceti infections have been increasingly reported in cetaceans, although a very limited characterization of Mediterranean Brucella spp. isolates has been previously reported and relatively few data exist about brucellosis among cetaceans in Italy. To address this gap, we studied 8 cases of B. ceti infection in striped dolphins (Stenella coeruleoalba) stranded along the Italian coastline from 2012 to 2018, investigated thanks to the Italian surveillance activity on stranded cetaceans. We focused on cases of stranding in eastern and western Italian seas, occurred along the Apulia (N = 6), Liguria (N = 1) and Calabria (N = 1) coastlines, through the analysis of gross and microscopic findings, the results of microbiological, biomolecular and serological investigations, as well as the detection of other relevant pathogens. The comparative genomic analysis used whole genome sequences of B. ceti from Italy paired with the publicly available complete genomes. Pathological changes consistent with B. ceti infection were detected in the central nervous system of 7 animals, showing non-suppurative meningoencephalitis. In 4 cases severe coinfections were detected, mostly involving Dolphin Morbillivirus (DMV). The severity of B. ceti-associated lesions supports the role of this microbial agent as a primary neurotropic pathogen for striped dolphins. We classified the 8 isolates into the common sequence type 26 (ST-26). Whole genome SNP analysis showed that the strains from Italy clustered into two genetically distinct clades. The first clade comprised exclusively the isolates from Ionian and Adriatic Seas, while the second one included the strain from the Ligurian Sea and those from the Catalonian coast. Plotting these clades onto the geographic map suggests a link between their phylogeny and topographical distribution. These results represent the first extensive characterization of B. ceti isolated from Italian waters reported to date and show the usefulness of WGS for understanding of the evolution of this emerging pathogen.
Complications and Complication Avoidance With Cervical Total Disc Replacement

ABSTRACT Complications with cervical arthroplasty can be generalized to errors in patient selection or surgical technique. Patients with advanced spondylosis or osteophytic disease, severe facet arthropathy, osteoporosis, sagittal deformity, or preoperative instability are poor candidates for arthroplasty and are more prone to complications. Poor surgical technique can result in subsidence, expulsion, and kyphosis, and it can contribute to heterotopic ossification. Additionally, all of the inherent complications from an anterior cervical approach may occur with cervical artificial disc placement. This article will focus on the complications uniquely associated with cervical arthroplasty.
Targeting FAAH and COX to Treat Visceral Pain

Genetic deletion or pharmacological inhibition of fatty acid amide hydrolase (FAAH), the primary enzyme responsible for catabolism of the endogenous cannabinoid anandamide, leads to a CB1 receptor mediated decrease in nociceptive behavior in a variety of inflammatory and neuropathic pain models. However, there are presently no published reports that have evaluated whether FAAH blockade reduces nociception in visceral pain, though direct‐acting CB1 receptor agonists as well as cyclooxygenase (COX) inhibitors are highly effective in visceral pain assays. Therefore, in the present study we evaluated whether FAAH blockade will block nociceptive behavior in the mouse acetic acid writhing model. Additionally, we examined whether co‐administration of a FAAH inhibitor and COX inhibitor would synergize to block visceral pain. FAAH (‐/‐) mice as well as wild type mice treated with FAAH inhibitors (i.e., URB597 and OL‐135) or the non‐selective COX inhibitor diclofenac displayed a decrease in acetic acid‐induced writhing. URB597 (1–10 mg/kg; ED50 = 2.1 mg/kg) and diclofenac (3–30 mg/kg; ED50 = 9.8 mg/kg) produced dose‐dependent antinociception in the writhing test. The CB1 receptor antagonist rimonabant blocked the analgesic effects in the FAAH‐compromised mice, but failed to attenuate the actions of diclofenac. Interactions between URB597 and diclofenac were analyzed using an isobolographic approach. Combined administration of URB59597 and diclofenac in ratios of 1:1 [ED50s = 0.24 and 1.18 mg/kg], 1:3 [ED50s = 0.28 and 4.30 mg/kg], or 3:1 yielded [ED50s = 0.44 and 0.72 mg/kg] yielded synergistic interactions. Taken together, the results of the present study suggest that FAAH represents a promising target to treat visceral pain and combination of FAAH inhibitors and NSAIDs may have greater therapeutic utility than either class of drug given alone.
Advancements in the use of Auger electrons in science and medicine during the period 2015–2019

Abstract Auger electrons can be highly radiotoxic when they are used to irradiate specific molecular sites. This has spurred basic science investigations of their radiobiological effects and clinical investigations of their potential for therapy. Focused symposia on the biophysical aspects of Auger processes have been held quadrennially. This 9th International Symposium on Physical, Molecular, Cellular, and Medical Aspects of Auger Processes at Oxford University brought together scientists from many different fields to review past findings, discuss the latest studies, and plot the future work to be done. This review article examines the research in this field that was published during the years 2015–2019 which corresponds to the period since the last meeting in Japan. In addition, this article points to future work yet to be done. There have been a plethora of advancements in our understanding of Auger processes. These advancements range from basic atomic and molecular physics to new ways to implement Auger electron emitters in radiopharmaceutical therapy. The highly localized doses of radiation that are deposited within a 10 nm of the decay site make them precision tools for discovery across the physical, chemical, biological, and medical sciences.
Hair‐pulling disorder (Trichotillomania): Etiopathogenesis, diagnosis and treatment in a nutshell

Hair‐pulling disorder (Trichotillomania) is a disabling mental disorder. Patient's behavior is characterized by the recurrent pulling of own hair with hair loss and a marked dysfunction in various areas of daily life. Trichotillomania is a relatively common disorder with pediatric onset, often associated with significant morbidity, comorbidity, and functional decline. Surprisingly, children or adolescents have been little studied in the research studies on the pathophysiology and psychopathology of trichotillomania. Furthermore, more evidences regarding the effective and evidence‐based pharmacological interventions for the treatment of this condition are encouraged. This narrative review will report on the etiopathogenesis and clinical manifestations of trichotillomania including criteria for diagnosis and treatment issues of this complex mental disorder.
Silver nanoparticle on aluminum mirror: active spectroscopy and decay rate enhancement

Recent advances in nanotechnology and optics have paved the way for new plasmonic devices. One of them are nanopatch antennas that are simple and, at the same time, effective devices for localizing the electromagnetic field on a scale of less than 10 nm and can be used in photonic integrated circuits as effective sources of photons, including single-photon sources. In the present study, we investigate the radiative characteristics of a submonolayer of colloidal CdSe/CdS quantum dots that form island structures in a resonator: a cubic silver nanoparticle on an aluminum mirror. For detecting plasmonic nanoparticles on glass or metal surfaces, we propose a new technique involving a tunable laser and a confocal microscope. We provide a comparative study of the luminescence enhancement factors for QDs in the NPAs upon off-resonance excitation and at a wavelength close to the resonance; a significant difference in the luminescence enhancement factors (by order of magnitude) is demonstrated. A 60-fold reduction in the spontaneous emission time, as well as an increase in the radiation intensity by a factor of 330, has been obtained in the experiments. The increase in the spontaneous emission rate demonstrated for the quantum dots is explained by the Purcell effect. Full-wave simulations of electromagnetic fields were carried out for the model of the developed nanopatch antenna; luminescence enhancement factors and radiative efficiencies were calculated as well.
Biased Neural Representation of Feature-Based Attention in the Human Frontoparietal Network

Selective attention is a core cognitive function for efficient processing of information. Although it is well known that attention can modulate neural responses in many brain areas, the computational principles underlying attentional modulation remain unclear. Contrary to the prevailing view of a high-dimensional, distributed neural representation, here we show a surprisingly simple, biased neural representation for feature-based attention in a large dataset including five human fMRI studies. Selective attention is a core cognitive function for efficient processing of information. Although it is well known that attention can modulate neural responses in many brain areas, the computational principles underlying attentional modulation remain unclear. Contrary to the prevailing view of a high-dimensional, distributed neural representation, here we show a surprisingly simple, biased neural representation for feature-based attention in a large dataset including five human fMRI studies. We found that when human participants (both sexes) selected one feature from a compound stimulus, voxels in many cortical areas responded consistently higher to one attended feature over the other. This univariate bias was consistent across brain areas within individual subjects. Importantly, this univariate bias showed a progressively stronger magnitude along the cortical hierarchy. In frontoparietal areas, the bias was strongest and contributed largely to pattern-based decoding, whereas early visual areas lacked such a bias. These findings suggest a gradual transition from a more analog to a more abstract representation of attentional priority along the cortical hierarchy. Biased neural responses in high-level areas likely reflect a low-dimensional neural code that can facilitate a robust representation and simple readout of cognitive variables. SIGNIFICANCE STATEMENT It is typically assumed that cognitive variables are represented by distributed population activities. Although this view is rooted in decades of work in the sensory system, it has not been rigorously tested at different levels of cortical hierarchy. Here we show a novel, low-dimensional coding scheme that dominated the representation of feature-based attention in frontoparietal areas. The simplicity of such a biased code may confer a robust representation of cognitive variables, such as attentional selection, working memory, and decision-making.
Activation of ventrolateral orbital cortex improves mouse neuropathic pain–induced anxiodepression

Depression and anxiety are frequently observed in patients suffering from neuropathic pain. The underlying mechanisms remained unclear. The ventrolateral orbital cortex (VLO) has attracted considerable interest in its role in antidepressive effect in rodents. In the present study, we further investigated the role of the VLO in the anxiodepressive consequences of neuropathic pain in a chronic constriction injury of infraorbital nerve–induced trigeminal neuralgia (TN) mouse model. Elevated plus maze, open field, forced swimming, tail suspension, and sucrose preference tests were used to evaluate anxiodepressive-like behaviors. The results show that chemogenetic activation of bilateral VLO neurons, especially CaMK2A+ pyramidal neurons, blocked the TN-induced anxiodepressive-like behaviors. Chemogenetic and optogenetic activation of VGLUT2+ or inhibition of VGAT+ VLO neurons was sufficient to produce an antianxiodepressive effect in TN mice. Pharmacological activation of D1-like receptors (D1Rs) but not D2Rs in the VLO significantly alleviated TN-induced depressive-like behaviors. Electrophysiological recordings revealed a decreased excitability of VLO excitatory neurons following neuropathic pain. Furthermore, activation of submedius thalamic nucleus–VLO (Sm-VLO) projection mimicked the antianxiodepressive effect of VLO excitation. Conversely, activation of VLO-periaqueductal gray matter (PAG) projection had no effect on TN-induced anxiodepressive behaviors. This study provides a potentially novel mechanism–based therapeutic strategy for the anxiodepressive consequences of neuropathic pain.
Clinical Activity and Safety of the Anti–Programmed Death 1 Monoclonal Antibody Dostarlimab for Patients With Recurrent or Advanced Mismatch Repair–Deficient Endometrial Cancer

Key Points Question What is the clinical antitumor activity and safety of dostarlimab for patients with deficient mismatch repair endometrial cancer? Findings In this nonrandomized phase 1 clinical trial, the confirmed objective response rate was 42%; 13% of patients had a confirmed complete response, and 30% of patients had a confirmed partial response. Anemia (3%), colitis (2%), and diarrhea (2%) were the most common grade 3 or higher treatment-related adverse events. Meaning Dostarlimab was associated with clinically meaningful and durable antitumor activity with an acceptable safety profile for patients with deficient mismatch repair endometrial cancers that have progressed after prior platinum-based chemotherapy.
Hydroperoxide Lyase Modulates Defense Response and Confers Lesion-mimic Leaf Phenotype in Soybean (Glycine max (L.) Merr.).

Allene oxide synthase (AOS) and hydroperoxide lyase (HPL) are two important members of P450 enzymes metabolizing hydroperoxy fatty acid to produce jasmonates and aldehydes respectively, which function in response to diverse environmental and developmental stimuli. However, their exact roles in soybean have not been clarified. In present study, we identified a lesion-mimic mutant in soybean named NT302, which exhibits etiolated phenotype together with chlorotic and spontaneous lesions on leaves at R3 podding stage. The underlying gene was identified as GmHPL encoding hydroperoxide lyase by map-based cloning strategy. Sequence analysis demonstrated that a single nucleotide mutation created a premature termination codon (Gln20-Ter), which resulted in a truncated GmHPL protein in NT302. GmHPL RNA was significantly reduced in NT302 mutant, while genes in AOS branch of the 13-LOX pathway were up-regulated in NT302. The mutant exhibited higher susceptibility to bacterial leaf pustule (BLP) disease, but increased resistance against common cutworm (CCW) pest. GmHPL was significantly induced in response to MeJA, wounding, and CCW in wild type soybean. Virus induced gene silencing (VIGS) of GhHPL genes gave rise to similar lesion-mimic leaf phenotypes in upland cotton, coupled with upregulation of the expression of JA biosynthesis and JA-induced genes. Our study provides evidence that competition exist between HPL and AOS branches in 13-LOX pathway of the oxylipin metabolism in soybean, thereby plays essential roles in modulation of plant development and defense.
Sifat Fisik Daging Sapi Dark Firm Dry (DFD) Hasil Fermentasi Bakteri Asam Laktat Lactobacillus plantarum

A dark firm dry beef (DFD) can be produced in meat from animal with a degree of stress susceptibility. This type of meat is very sticky in texture and not palatable. The aim of the research was to study physical properties of DFD beef fermented by Lactobacillus plantarum combined with cold smoking. The experiment used a randomized block design. DFD beef samples were collected from beef abatoar with pH value of 6.5 - 6.8. The samples were divided into two groups; fermented by Lactobacillus plantarum and fermented naturally (control) for five days fermentation. Fermentation process was combined with cold smoking. The result showed that the pH value, tenderness and color of both groups were significantly influenced by the treatment, but the water holding capacity was not different. DFD beef fermented by Lactobacillus plantarum was more tender, lighter color and had longer sarcomere than control. Sensory analysis showed that color and tenderness of DFD beef fermented by Lactobacillus plantarum were better than control. Key words : dark firm dry beef, fermentation, Lactobacillus plantarum, color, tenderness
Recent Progress of Nanocarrier-Based Therapy for Solid Malignancies

Simple Summary Although conventional anti-cancer drugs have been the footstone in the fight against cancer, yet they are far from optimal due to issues related with indiscriminate destruction of normal cells, multidrug resistance, and toxicity, as a result, a more selective therapy is urgently needed. Nanocarriers have been increasingly used in drug delivery, especially in cancer therapy. Nanocarriers can improve the therapeutic effect of drugs in cancer by enhancing the specificity and prolonging the circulating half-life of drugs. The aim of this review is to offer a detailed description of different cytotoxic drug nanocarriers and their recent progress. It is expected that this review will be of help to those who have been seeking new study directions in this field and also ones who are about to start the research on nanocarrier-based drug delivery. Abstract Conventional chemotherapy is still an important option of cancer treatment, but it has poor cell selectivity, severe side effects, and drug resistance. Utilizing nanoparticles (NPs) to improve the therapeutic effect of chemotherapeutic drugs has been highlighted in recent years. Nanotechnology dramatically changed the face of oncology by high loading capacity, less toxicity, targeted delivery of drugs, increased uptake to target sites, and optimized pharmacokinetic patterns of traditional drugs. At present, research is being envisaged in the field of novel nano-pharmaceutical design, such as liposome, polymer NPs, bio-NPs, and inorganic NPs, so as to make chemotherapy effective and long-lasting. Till now, a number of studies have been conducted using a wide range of nanocarriers for the treatment of solid tumors including lung, breast, pancreas, brain, and liver. To provide a reference for the further application of chemodrug-loaded nanoformulations, this review gives an overview of the recent development of nanocarriers, and the updated status of their use in the treatment of several solid tumors.
Preparing students for the future through developing evaluative judgement

In today’s environment, health students need to be prepared for an ever changing workplace and workforce. They need to understand what demonstrates good quality work and how to assess their standard of work. Evaluative judgement can contribute to student and graduate self‐regulation and autonomy in their learning. This toolbox article describes how to implement strategies for developing evaluative judgement in allied health placements. These strategies were developed as part of an occupational therapy program in rural Western Australia, where experiential education is provided through service‐learning with both direct and indirect supervision. Practical advice is provided on the use of peer‐assisted learning, rubrics, self‐assessment and feedback. Challenges and opportunities in implementing strategies to develop evaluative judgement, such as how to achieve effective feedback, are also discussed. The suggested toolbox may be adapted to various clinical placement contexts. Developing evaluative judgement can help to prepare our students to be lifelong learners. This article empowers educators to promote this capability in their students to create work ready graduates, who are able to operate independently and in rapidly evolving, technologically enabled environments
Decision Making by Drosophila Flies

“Decision making has all the secrets of everything: who we are, what we do, how we navigate the world. ” “How Do I Decide? The Brain with David Eagleman”, 2015. When presented with attractant (light) together with an amount of repellent (methyl eugenol) that exceeds attractant, Drosophila melanogaster fruit flies are of course repelled, but nine mutants have now been isolated that were not repelled. Although able to respond to attractant alone and to repellent alone, these mutants fail to make a decision when the two are together during the first two months of the study. They are considered defective in a decision-making mechanism. The defect occurs at 34°C but not at room temperature, so these are conditional mutants. Efforts at genetic mapping have been made. Our aim is to discover how decision making gets accomplished and how this results in a behavioral response. We indicate that there is a mechanistic relationship between decision making and the central complex in Drosophila and between decision making and the prefrontal cortex in humans and other vertebrates. Over a period of six months these mutants changed into ones that are attracted when presented with attractant together with what was overpowering repellent before. Nearly full attraction was achieved at fifteen to thirty days. With attractant alone these mutants were attracted like the original parent and with repellents alone they were repelled like the original parent. The mutants have been genetically mapped.
A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage‐derived IncY plasmid

The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K‐12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K‐12, the 4.88 Mbp Fec10 genome is characterized by distinct single‐nucleotide polymorphisms and acquisition of genomic islands. In addition, E. coli Fec10 possesses a 155.86 kbp IncY plasmid, a composite element based on phage P1. pFec10 harbours multiple cargo genes such as coding for a tetrathionate reductase and its corresponding regulatory two‐component system. Among the cargo genes is also the Transmissible Locus of Protein Quality Control (TLPQC), which mediates tolerance to lethal temperatures in bacteria. The disaggregase ClpGGI of TLPQC constitutes a major determinant of the thermotolerance of E. coli Fec10. We confirmed stand‐alone disaggregation activity, but observed distinct biochemical characteristics of ClpGGI‐Fec10 compared to the nearly identical Pseudomonas aeruginosa ClpGGI‐SG17M. Furthermore, we noted a unique contribution of ClpGGI‐Fec10 to the exquisite thermotolerance of E. coli Fec10, suggesting functional differences between both disaggregases in vivo. Detection of thermotolerance in 10% of human commensal E. coli isolates hints to the successful establishment of food‐borne heat‐resistant strains in the human gut.