Miracle-dz/UniFAR-FTUs huggingface dataset:
Susceptibility to late onset hearing loss: an investigation into genetic variation at the Brn-3c locus.

BrnSc (BrnS.l, POU4F3) encoding a POU domain transcription factor is a candidate gene for late onset sensorineural hearing loss, which is exhibited by a large proportion of the ageing population. To identify common sequence variants at the Brn-3c locus mutation scanning of the BrnSc cDNA, intron and 5'-flanking region was performed by PCR-SSCP analysis in 45 members of the general population. Seven polymorphic sites were identified of which five within the Bm-Sc 5'-flanking region appear common. A functional screening approach utilising in-vitro assays suggests that at least three common sequence variants in the Brn-Sc 5'-flanking region could have a functional affect: -566(GT)i7-23, -1391A>C and a complex multi-allelic poly-G polymorphism at - 3432 that exhibits multiple variations in length together with single base substitutions within the guanine repeat. The -3432poly-G polymorphism modifies the binding affinity of an OC-2 derived nuclear protein and there is convincing evidence that this is the transcription factor SP1. Use of purified human recombinant SP1 protein, in-vitro translated SP1 and in-vitro translated SP3 confirms that the -3432polyG polymorphism modulates a high affinity SP family binding site and evidence suggests that this alters the regulation of the BrnSc promoter when SP1 levels are limiting, p<0.05. Moreover, the data suggest a functional interaction between the -3432poly-G polymorphism and the -566(GT)i7.23 repeat which associate to determine the response of the Brn-3c gene to SP1. Similarly, evidence suggests that the variant allele, -1391C has a reduced affinity for an OC-2 derived nuclear protein and this is consistent with a significant decrease in basal activity of the Brn-Sc promoter, pC were genotyped for a pilot association study but allelic frequencies were not found to significantly differ between the patient and control populations examined (by %2 analysis). Further large-scale population studies are required to establish whether these common sequence variants are associated with late onset hearing loss.
Application of turbiscan lab to study the effect of emulsifier content on the stability of plant origin dispersion

The eﬀect of emulsiﬁer volume on emulsion system stability of plant origin being the basis of diet supplements for animals in winter season was analyzed. For this purpose, measurements of the backscattered light intensity as the function of the measuring cell height were conducted with a Turbiscan LAB optical analyzer. System stability was analyzed on the basis of Turbiscan Stability Index values. A Helos laser analyzer and a Nikon Eclipse E400 POL optical microscope were used to investigate drop size distribution and analyze microscopic pictures. It was shown that emulsion with 10% (w/w) of the emulsiﬁer was the most stable one.
Awareness of sexually transmitted infections in Poland

Sexually transmitted infections (STIs) spread through sexual contact and can be responsible for many clinically significant complications, such as pelvic inflammatory disease, infertility, ectopic pregnancy, fetal or neonatal death, premature delivery and increased susceptibility to HIV infection. Worldwide, millions of cases of STIs, such as chlamydia, syphilis or gonorrhoea occur every year, and hundreds of millions of people are currently infected. Some STIs can be cured following appropriate therapy. Others cannot be completely cured, but medicines can be used to manage symptoms and prevent ongoing transmission. According to the World Health Organization, STIs are one of the five types of diseases for which adults most commonly seek medical support worldwide. In this paper, we present the results of the analysis of data collected in Poland on the awareness of Polish society about sexually transmitted infections.
Horizontal distribution of ciliated protozoa between the Sphagnum mat and open water zone in a shallow peat-bog pools

Summary. The aim of this study was to examine the community structure and horizontal microdistribution (under the peat mat, transitional zone peat mat/open water and open water zone) of ciliates in shallow peat-bog pools in eastern Poland, as well as to assess the effect of physical and chemical factors on the distribution of planktonic ciliates. The highest species richness occurred in the transitional zone. Decidedly lower numbers of taxa were observed in the open water zone. The density and biomass of protozoa significantly differed between the studied zones, with the lowest numbers in the open water zone and the highest between peat mat and open water. Analyses at the level of species and genera revealed distinct distribution patterns of some taxa. Strombidium viride and Vorticella companula as medium-sized showed highest abundance in open water. Small and medium-sized Cinetochilum margaritaceum and Colpoda sp. were highly abundant in the transitional and in the peat mat zones. In the peat mat, as well as in contact zone, the number of ciliates had the strongest correlation with water temperature, conductivity, concentrations of total organic carbon. In turn, in the open water zone, there was a significant rise in the strong correlation between the number of ciliates and the total phosphorus concentration.
Understanding intestinal lipopolysaccharide permeability and associated inflammation

Lipopolysaccharide (LPS) and the inflammation associated with its stimulation of the innate immune responses can have major implications for human and animal health and production. This dissertation research goal was to further understand dietary modulation of intestinal LPS permeability and LPS associated inflammation. Additionally, we sort to examine LPS detoxification and the relationship LPS has with swine health and feed efficiency. High caloric and high dietary fat increases the risk of endotoxemia which can result in a low grade inflammation, a predisposing factor for common metabolic diseases such as Type II diabetes and atherosclerosis. However, little is known about the effect of dietary oil fatty acid composition on intestinal LPS permeability and postprandial endotoxemia. Therefore, we examined whether dietary oil composition differentially modulated intestinal LPS permeability and postprandial endotoxemia. Our in vivo and ex vivo research using pigs and isolated pig intestinal tissues indicated that a single administration of oils rich in long chain n-3 polyunsaturated fatty acids (PUFA), such as fish oil and cod liver oil, decreases LPS permeability and postprandial circulating LPS levels (P<0.05). Furthermore, oils rich in saturated fatty acids, such as coconut oil, augmented LPS permeability and postprandial endotoxemia (P<0.05). Mechanistically, this may be associated with the structure and function of cell membrane lipid raft microdomain structures. Dietary long chain n-3 PUFA such as eicosapentaenoic acid (EPA) and docoasahexaenoic acid (DHA) have been shown to antagonize LPS signaling. Therefore, we
Secagem da polpa de goiaba (psidium guajava) da variedade Pedro Sato pelo método foam mat drying para aplicação em alimentos

A goiaba (psidium guajava) e uma fonte de vitamina C, licopeno, β-caroteno e fenois, os quais tem propriedades antioxidantes. Porem, devido a sua alta perecibilidade uma grande parcela da goiaba colhida e perdida antes de chegar ao consumidor. Por isso, alguns metodos de preservacao precisam ser utilizados, e o metodo de desidratacao por foam mat drying possui grande potencial para preservacao desta fruta. O processo de secagem por foam mat drying para a polpa de goiaba foi avaliado variando diferentes concentracoes de lecitina de soja e carboximetilcelulose como agentes estabilizadores de espuma na temperatura de secagem de 80 °C. As analises de caracterizacao assim como os parâmetros de cor foram avaliados na polpa de goiaba desidratada em todos os ensaios de secagem, no intuito de verificar a melhor condicao de secagem. A analise estatistica indicou diferenca significativa (p < 0,05) para o conteudo de vitamina C, fenois totais e capacidade antioxidante pela influencia da concentracao de carboximetilceululose e lecitina de soja. No entanto, houve aumento significativo para a capacidade antioxidante e fenois totais na polpa de goiaba desidratada em todas as condicoes avaliadas em comparacao com a polpa in natura. O estudo concluiu que a condicao de secagem com a concentracao de 0,15 g de Carboximetilcelulose e 0,15 g de Lecitina de Soja / 100 g apresentou menor tempo de secagem e concomitantemente, maiores valores para fenois totais, capacidade antioxidante e melhor retencao da vitamina C em comparacao com as outras condicoes de secagem avaliadas.
An Artificial Immune System for Solving Dynamic Economic Power Dispatch Problems

In this paper, we propose an artiﬁcial i mm une syste m called IA DED, which stands for Immune Algorithm Dynamic Economic Dis-patch . It is designed for solving the Dyna m ic Econo m ic Dispatch (DED) proble m . Our approach considers the DED proble m as a dyna m ic prob-le m whose constraints change over ti m e. IA DED considers the activation process that T cells suﬀer in order to ﬁnd partial solutions. The proposed approach is validated using several DED proble m s taken fro m the specialized literature. Our results are co m pared with respect to those obtained by other approaches taken fro m the specialized literature. We also provide so m e statistical analysis in order to deter m ine the sensitivity of the perfor m ance of our proposed approach to its para m eters.
Rab GTPases in Autophagy

Rab proteins constitute a subfamily of small GTPases that play important roles in the spatio‐ temporal regulation of intracellular vesicle transport [1-3]. Rab GTPases represent a large family of small guanosine triphosphate (GTP)-binding proteins that comprise more than 60 known members. In mammalian cells, it is well established that different Rab proteins localize on distinct membrane-bound compartments, where they regulate multiple steps in membrane traffic, including vesicle budding, fusion, and movement, through cycling between an inactive guanosine diphosphate (GDP)-bound form and an active GTP-bound form [3]. Guanine nucleotide exchange factor (GEF) shifts GTPase from its inactive GDP-bound form to its active GTP-bound form, while GTPase activating domain protein (GAP) inactivates GTPase [Figure 1]. Many structural and biological studies have shown that specific amino acid mutation can make possible to keep Rab GTPase in its GDP-bound form or GTP-bound form; therefore, expression of GDP-bound form or GTP-bound form could imitate its function [3].
Moroccan Foreign direct investment and Human development in ECOWAS countries: an econometric modeling using dynamic panel cointegration panel approach.

This work establishes an empirical relationship between Moroccan foreign direct investment (FDI) and the social and human development (HDI) of ECOWAS member countries in the context of the bivariate cointegration approach with dynamic panel data and Causality at meaning of Granger over the period 2002-2015. The result supports evidence of panel cointegration between FDI and HDI. As a result, we have found that FDI has a positive impact on social and human development in the long term. The results also support evidence of long-term causality ranging from HDI to FDI and from FDI to HDI in the short and long term. Our empirical results support the idea that South-South cooperation benefits both parties in the framework of Co-development.
Cell lineage specification and patterning of the embryo

Specification of the Cell Lineages: Determinants of Cell and Positional Fate in Ascidian Embryos, W.R. Jeffery. RNA Localization and Germ Cell Determination in Xenopus, M. Kloc, S. Bilinski, A. P-Y. Chan, L.H. Allen, N.R. Zearfoss, and L.D. Etkin. Asymmetric Germ Cell Division and Oocyte Determination during Drosophila Oogenesis, W. Deng and H. Lin. The Germline in C. elegans: Origins, Proliferation, and Silencing, G. Seydoux and T. Schedl. Assembly of the Drosophila Germ Plasm, A.P. Mahowald. Early Events in Mammalian Germ Line, R. Anderson, J. Heasman, and C. Wylie. Patterning of the Embryo: The Initial Phase of Embryonic Patterning in Mammals, R.L. Gardner. Cadherin and Catenins, Wnts and Soxs: Embryonic Patterning in Xenopus, A.L. St. Amand and M.W. Klymkowsky. Establishment of Left-Right Asymmetry, H.J. Yost. Initiation and Early Patterning of the Endoderm, D. Clements, M. Rex, and H.R. Woodland. Neural Patterning in the Vertebrate Embryo, C.R. Altmann and A. Hemmati-Brivanlou. Cell Biology of Limb Patterning, S.A. Schaller, S. Li, V. Ngo-Muller, M. -J. Han, M. Omi, R. Anderson, and K. Muneoka. Mechanisms to Establish Polarity and Initiate Cell Fate Determination: Defining Cis-Acting Elements and Trans-Acting Factors in RNA Localization, K. Yaniv and J.K. Yisraeli. RNA Localization and Translational Regulation during Axis Specification in the Drosophila Oocyte, R.L. Cooperstock and H.D. Lipshitz. Translational Control in Vertebrate Development, C.H. de Moor and J.D. Richter. Chapter References. Index.
THE CLUSTER AS A SOCIALLY RESPONSIBLE ENTITY

Corporate Social Responsibility (CSR) is gaining more and more importance. 4 It involves activities that is associated with the organization’s ethical behavior towards society, 5 in particular its recipients, employees, other stakeholders as well as its impact on the 6 environment. Universities deserve special attention in the field of CSR activities which play 7 a huge role in shaping society, its development and are source of information for it. The aim of 8 the paper is to analyze the three examples of polish clusters in the social responsibility context. 9 In the paper the authors also described short history of clusters, their main ideas and the 10 conception of CSR in the theoretical aspect. 11
Artificial marine structures facilitate the spread of a non‐indigenous green alga, Codium fragile ssp. tomentosoides, in the north Adriatic Sea

Summary 1 Artificial structures have become ubiquitous features of coastal landscapes. Although they provide novel habitats for the colonization of marine organisms, their role in facilitating biological invasions has been largely unexplored. 2 We investigated the distribution and dynamics of the introduced green alga, Codium fragile ssp. tomentosoides, at a variety of spatial scales on breakwaters in the north Adriatic Sea, and analysed experimentally the mechanisms underlying its establishment. We assessed the provision of sheltered habitats by breakwaters, the role of disturbance (e.g. from recreational harvesting and storms) acting at different times of the year, and the interactions between Codium and the dominant native space-occupier, the mussel Mytilus galloprovincialis. 3 Codium fragile ssp. tomentosoides has established viable populations on artificial structures along the shores investigated. The density, cover and size (length, branching and weight) of annual erect thalli of Codium was enhanced in sheltered conditions, resulting in the monopolization of landward low-shore habitats of breakwaters. 4 On the landward sides of breakwaters, disturbance enhanced recruitment of Codium. The time when bare space was provided within mussels beds was crucial. Removal of mussels in April or January did not affect the recruitment of Codium, whereas harvest in August, shortly before Codium gamete release, doubled its success. On the seaward sides of breakwaters, the effects of disturbance were more complex because mussels both inhibited recruitment of Codium and provided shelter from wave action to adult thalli. 5 Synthesis and applications. Artificial structures can provide suitable habitats for non-indigenous marine species and function as corridors for their expansion. Physical (wave exposure) and biotic (resident assemblages) features of artificial habitats can be important determinants of their susceptibility to biological invasions. Alternative options in the design of artificial structures and effective management of native assemblages could minimize their role in biological invasions. In particular, increased water motion and retention of space by mussels in spring–summer would be effective in reducing the ability of C. fragile ssp. tomentosoides to persist on the breakwaters investigated in this study.
Synthesis of Fluorescent 4-Methyl-7-thiocoumaryl S-Glycosides of Sialic Acid

Condensation of 4-methyl-7-thiocoumarin sodium salt with methyl 5-N-acetyl-4, 7, 8, 9-tetra-O-acetyl-2-chloro-2, 3, 5-trideoxy-D-glycero-D-galacto-2-nonulopyranosonate (2), methyl 5-N-(O-acetylglycolyl)-4, 7, 8, 9-tetra-O-acetyl-2-chloro-2, 3, 6-trideoxy-D-glycero-D-galacto-2-nonulopyranosonate (11), and methyl 4, 5, 7, 8, 9-penta-O-acetyl-2-chloro-2, 3-dideoxy-D-glycero-D-galacto-2-nonulopyranosonate (14) under Williamson reaction conditions gave the corresponding α-glycosides in good yields. Deprotection of these α-glycosides gave three new fluorogenic substrates, the 4-methylcoumarin-7-yl S-glycosides of N-acetylneuraminic acid, N-glycolylneuraminic acid, and 3-deoxy-D-glucose-D-galacto-2-nonulopyranosonic acid (KDN). Furthermore, we have developed a facile method for preparation of benzyl 5-amino-3, 5-dideoxy-α-D-glycero-D-galacto-2-nonulopyranosidonic acid (7), a key intermediate for the synthesis of N-glycolylneuraminic acid.
Target-dependent amplifiable nucleic acid hybridization probes

• A submitted manuscript is the author's version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website. • The final author version and the galley proof are versions of the publication after peer review. • The final published version features the final layout of the paper including the volume, issue and page numbers.
Bunt kobiet : czarne protesty i strajki kobiet

Celem serii RAPORT jest interpretacja wspolczesnych problemow spoleczenstwa obywatelskiego, a takze diagnoza przeobrazen kultury politycznej i wspolpracy miedzynarodowej w Polsce i Europie. Pragniemy przemyślec zmiany, ktore zachodzą wlaśnie na naszych oczach, i poddac interpretacji procesy przemian ostatnich dziesiecioleci, ktore wplynely na uksztaltowanie sie demokracji w regionie. Seria prezentuje efekty debat publicznych i projektow badawczych zainicjowanych i realizowanych przez Europejskie Centrum Solidarności we wspolpracy z instytucjami partnerskimi. Naszą intencją jest, by wydawane na wolnych licencjach Raporty sluzyly szerokiemu kregowi czytelniczek i czytelnikow oraz inspirowaly do ksztaltowania nowej solidarnej kultury w spoleczenstwach Europy. SPIS TREŚCI: KALENDARIUM || WSTĘP: Mobilizacja kobiet w latach 2016–2018: przyczyny, konteksty i perspektywy badawcze || Beata Kowalska, Radoslaw Nawojski : ROZDZIAŁ 1 - Uwaga, uwaga, tu obywatelki! Obywatelstwo jako praktyka w Czarnych Protestach i Strajkach Kobiet || Jennifer Ramme, Claudia Snochowska-Gonzalez: ROZDZIAŁ 2 - Nie/zwykle kobiety. Populizm prawicy, wola ludu a kobiecy suweren || Elzbieta Korolczuk: ROZDZIAŁ 3 Odzyskiwanie jezyka, czyli jak zmienila sie debata o aborcji w kontekście Czarnych Protestow i Strajkow Kobiet || Jenny Gunnarsson Payne: ROZDZIAŁ 4 - Kobiety jako „lud”. Czarne Protesty jako sprzeciw wobec autorytarnego populizmu w perspektywie miedzynarodowej|| DODATEK: Przemowienia ||Archiwum spoleczne || O SERII RAPORT || BIOGRAMY.
FUNÇÃO DO CÁLCIO NA DEGRADAÇÃO DA PAREDE CELULAR VEGETAL

EnglishCalcium is one of the most important intracellular system of plants, it remains in ionic form while inside the cell, acting on the secondary metabolism of all cells. It is an element that plays different roles in plant tissues, especially in protecting the cell walls and membranes and signaling in response to changing biotic and abiotic stress. The softening of the fruit during its ripening involves changes in cell wall polysaccharides. Calcium bridges between pectic acids, or between these and other polysaccharides hinder access and the action of pectinolytic enzymes produced by the fruit and cause softening, and those produced by fungi and bacteria that cause decay. Calcium in plant cell wall, to covalently bind to pectins, gives rise to calcium pectate, restricting the action of polygalacturonase and pectin, and thus slowing the softening of fruits. The application of calcium both in spray and in the fruit results in grave: the preservation of fruit firmness, reduction of respiration rate of respiration, reduced ethylene production, the lower activity of β-galactosidase, an increase of hemicellulose, pectins and calcium in the pulp . In fruits, the effect of calcium has received much attention, since the applications of this cation promotes the retardation of ripening and senescence, prolonging the life and shelf life. portuguesO calcio e um dos elementos mais importantes no sistema intracelular de plantas, pois permanece na forma ionica quando dentro da celula, atuando no metabolismo secundario de todas as celulas. E um elemento que desempenha diferentes funcoes nos tecidos vegetais, notadamente na protecao das membranas e paredes celulares e na sinalizacao de resposta as condicoes de estresse biotico ou abioticos. O amaciamento de frutos durante o seu amadurecimento implica em modificacoes de polissacarideos da parede celular. As pontes de calcio entre os acidos pecticos ou entre esses e outros polissacarideos dificultam o acesso e a acao de enzimas pectoliticas produzidas pelo fruto e que causam amaciamento, e daquelas produzidas pelos fungos e bacterias que causam deterioracao. O calcio na parede celular vegetal, ao ligar-se covalentemente as pectinas, da origem ao pectato de calcio, restringindo a acao da pectinametilesterase e poligalacturonase, e, consequentemente, retardando o amaciamento de frutos. A aplicacao de calcio tanto em pulverizacao nos frutos quanto em cova resulta em: preservacao da firmeza do fruto, reducao da respiracao da taxa respiratoria, reducao da producao de etileno, menor atividade da β-galactosidase, aumento de hemicelulose, pectinas e calcio na polpa. Em frutos, o efeito do calcio tem recebido muita atencao, visto que as aplicacoes deste cation promovem o retardamento da maturacao e da senescencia, prolongando o periodo de vida util e o tempo de prateleira.
Bio-available amino acids extraction from soil by demineralized water and 0.5M ammonium acetate Short Communication

Summary. The extraction and comparison of soil bio-available amino acids using either demineralised water (DEMI H2O) or 0.5 M ammonium acetate (0.5 M AAc) solution is reported. Results show that the extraction by 0.5 M AAc is a better method to assess the concentration of bioavailable amino acids in soil than DEMI H2O due to higher extraction efficiency and better amino acid protection against microbial degradation during processing.