Miracle-dz/UniFAR-FTUs huggingface dataset:
Repertoire and structure of duet and solo songs in cooperatively breeding white-browed sparrow weavers

Summary White-browed sparrow weavers (Plocepasser mahali) are cooperatively breeding songbirds of eastern and southern Africa that live in small groups year-round consisting of a dominant breeding pair, and subordinate, non-breeding males and females. This species possesses an elaborate vocal communication system: solo songs are exclusively produced by the dominant male, duet songs are mainly produced by the dominant pair, and chorus songs, similar in syllable structure and temporal pattern to duet songs, are produced by all group members. We analysed songs of males and females with known social status recorded from different colonies in Zimbabwe and complemented these data by studying songs of captive birds at our institute. Solo songs are produced in one performance at dawn during the breeding season. Recordings of captive males on consecutive days revealed that males sing in a single solo performance 88.4 ± 4.1% of their total solo song repertoire. This suggests that dominant males recorded in Zimbabwe have a solo song repertoire of 67.0 ± 4.0 syllables, which is similar in size to those of captive males (58.3 ± 3.7 syllables). Repertoire sizes of both free-living and captive males are not correlated with the length of the solo song performance. Duetting is both antiphonal and in unison. Dominant males and females appear to have similar sized duet repertoires (51.9 ± 2.1 syllables). Recordings from captive pairs suggest that 7598% of the repertoire is shared with higher syllable sharing in more experienced pairs. Since all group members engage in duets and chorus singing, we estimate that each subordinate male and female shares the duet syllable repertoire with the breeding pair. For dominant males, the duet syllables are widely distinct from those of the solo songs; of their total syllable repertoire only 2.1% occur in both repertoires. Further, solo song and duet song differ in the temporal organisation.
New aryl Schiff bases of thiadiazole derivative of ibuprofen as DNA binders and potential anticancer drug candidates

Abstract The work presented in this paper describes the synthesis of two new aryl Schiff bases [(E)-N-(4-(benzyloxy)-3-methoxybenzylidene)-5-(1-(4-isobutylphenyl)ethyl)-1,3,4-thiadiazol-2-amine] (ASB-1) and [(E)-N-(4-(benzyloxy)benzylidene)-5-(1-(4-isobutylphenyl)ethyl)-1,3,4-thiadiazol-2-amine] (ASB-2). These compounds were characterized by different analytical techniques and then studied for DNA binding. Binding studies were carried out at neutral pH (7.0) and at 37 °C by theoretical and experimental methods including DFT, molecular docking, spectroscopy (UV-visible, fluorescence), cyclic voltammetry (CV) and viscometry. Further investigations of these compounds were done on hepatocellular carcinoma; Huh-7 cancer cell line. Binding constant, free energy change and binding site size, i.e. Kb, ΔG and n were evaluated which indicated that both ASB-1 and ASB-2 bind significantly and spontaneously with the DNA. However, data revealed relatively greater binding of ASB-1 with DNA. Spectral and voltammetric results were found supportive of each other. Binding site sizes and viscosity measurements verified the mixed binding mode of interactions as observed in molecular docking analysis, i.e. intercalation with groove binding. DNA binding studies were very well correlated with the in-vitro studies performed on Huh-7 cell line as well as normal HEK-293 cell lines. The compound ASB-1 not only showed greater binding affinity toward DNA but also showed greater anticancer potency with least IC50 value as compared to ASB-2. Graphical Abstract Highlights New aryl Schiff bases of thiadiazole derivative of ibuprofen ASB-1 and ASB-2 Reactivity and mixed binding mode of interaction with DNA by DFT and docking Good agreements in the binding parameters by spectroscopy and voltammetry Kb, ΔG and n determined greater for ASB-1indicating its stronger binding with DNA Greater anticancer potential of ASB-1against Huh-7 cell line Communicated by Ramaswamy H. Sarma
Profiles of Teen Dating Violence and Association With Depression Among Chinese Teens

Teen dating violence (TDV) is a major global public health concern. Few studies, however, have examined profiles of TDV in Chinese societies and how these profiles might be associated with teens’ mental health. The current study analyzed a sample of 891 middle and high school students with dating experience in Taiwan, Hong Kong, and Shanghai. Latent class analysis (LCA) and multinomial logit regression analysis were performed in an attempt to identify profiles of TDV and then investigate possible associations between class membership and self-reported depression. The results of LCA suggested that a four-class model was the best fit for the data: Severe/Multi-Type TDV (5.51%), Controlling Behavior (13.08%), Non/Low TDV (64.50%), and Physical Violence (16.91%). The best-fit model suggested bidirectionality, meaning among teen partners in an abusive relationship, both tended to participate in violent acts and controlling behaviors. The results of the multinomial regression showed that, compared with the Non/Low TDV class, teens in the Severe/Multi-Type TDV class or Controlling Behavior class had greater odds of screening positive for depression. There was no significant difference in the risk of depression between the Physical Violence class and the Non/Low TDV class. Implications for future research and practice are also discussed herein.
Prp5−Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription

Abstract Transcription and pre-mRNA splicing are coupled to promote gene expression and regulation. However, mechanisms by which transcription and splicing influence each other are still under investigation. The ATPase Prp5p is required for pre-spliceosome assembly and splicing proofreading at the branch-point region. From an open UV mutagenesis screen for genetic suppressors of prp5 defects and subsequent targeted testing, we identify components of the TBP-binding module of the Spt–Ada–Gcn5 Acetyltransferase (SAGA) complex, Spt8p and Spt3p. Spt8Δ and spt3Δ rescue the cold-sensitivity of prp5-GAR allele, and prp5 mutants restore growth of spt8Δ and spt3Δ strains on 6-azauracil. By chromatin immunoprecipitation (ChIP), we find that prp5 alleles decrease recruitment of RNA polymerase II (Pol II) to an intron-containing gene, which is rescued by spt8Δ. Further ChIP-seq reveals that global effects on Pol II-binding are mutually rescued by prp5-GAR and spt8Δ. Inhibited splicing caused by prp5-GAR is also restored by spt8Δ. In vitro assays indicate that Prp5p directly interacts with Spt8p, but not Spt3p. We demonstrate that Prp5p's splicing proofreading is modulated by Spt8p and Spt3p. Therefore, this study reveals that interactions between the TBP-binding module of SAGA and the spliceosomal ATPase Prp5p mediate a balance between transcription initiation/elongation and pre-spliceosome assembly.
Variations on a theme: Morphological variation in the secondary eye visual pathway across the order of Araneae

Spiders possess a wide array of sensory‐driven behaviors and therefore provide rich models for studying evolutionary hypotheses about the relationship between brain morphology, sensory systems, and behavior. Despite this, only a handful of studies have examined brain variation across the order of Araneae. In this study, I present descriptions of the gross brain morphology for 19 families of spiders that vary in eye morphology. Spiders showed the most variation in the secondary eye visual pathway. Based on this variation, spiders could be categorized into four groups. Group 1 spiders had small, underdeveloped laminae, no medullae, and no mushroom bodies. Group 2 spiders had large laminae, no medullae and large mushroom bodies. Group 3 spiders had laminae and some evidence of reduced medullae and mushroom bodies. Group 4 spiders had the most complex systems, with large laminae, medullae formed from optical glomeruli, and robust mushroom bodies. Within groups, there was large variation in the shape and size of individual regions, indicating possible variation in neuronal organization. The possible evolutionary implications of the loss of a dedicated olfactory organ in spiders and its effects on the mushroom body are also discussed.
Vapor Sensing with Polymer Coated Straight Optical Fiber Microtapers Based on Index Sensitive Interference Spectroscopy of Surface Stress Birefringence

Optical microfiber tapers provide an advantageous platform for sensing in aqueous and gas environments. We study experimentally the photonic transmission in optical fiber tapers coated with polymethyl methacrylate (PMMA), a polymeric material widely used in optical applications. We demonstrate a durable and simple humidity sensing approach incorporating tapered microfibers attached to silicon (Si) substrate coated with active polymer layer. A model is described for the load stress effect on the birefringence giving rise to interferences in the transmission spectra, strongly dependent on the coating layer thickness, and disappearing following its slow uniform removal. The sensing approach is based on characterization of the interference patterns observed in the transmission spectra of the taper in the NIR range. The device demonstrated persistent detection capability in humid environment and a linear response followed by saturation to calibration analytes. In each analyte of interest, we define principal components and observe unique calibration plot regimes in the principal component space, demonstrating vapor sensing using polymer coated microtapers.
Comparison of Skin Lesions Caused by Ixodes ricinus Ticks and Lipoptena cervi Deer Keds Infesting Humans in the Natural Environment

Background: The territorial expansion and increased population size of haematophagous arthropods (i.e., the castor bean tick Ixodes ricinus (Ixodida: Ixodidae) and the deer ked Lipoptena cervi (Diptera: Hippoboscidae)) has enhanced the risk of human infestations in Europe. The aim of our study was to present skin lesions induced by tick and deer ked bites in patients from recreational forest regions in southeastern Poland and pay attention to features of skin changes that may be useful in differential diagnosis. Methods: We compare the skin lesions after I. ricinus and L. cervi bite and draw attention to the biological and ecological traits of both ectoparasites, which may be diagnostically relevant for determination of the cause of skin symptoms reported by patients. Results: I. ricinus bites lead to development of erythematous-infiltrative poorly demarcated lesions with a centrally located bite mark, which usually disappears within one to several days. In turn, L. cervi bites leave irregularly shaped scattered erythematous papules. The papules may persist for up to one year and are accompanied by itching. Conclusions: Correct assessment of the clinical picture and its association with an arthropod bite (e.g., tick or deer ked) is highly important for further diagnostic procedures (i.e., differentiation of skin lesions developing in tick-borne diseases and, consequently, correct choice of pharmacological therapy). I. ricinus and L. cervi differ in their developmental cycles and rhythms of activity, which indicates that both species should be considered potential causative agents in the differential diagnosis of skin lesions when the patient has been bitten by an arthropod in autumn and winter months.
Resource Management in Cloud Radio Access Network: Conventional and New Approaches

Cloud radio access network (C-RAN) is a promising mobile wireless sensor network architecture to address the challenges of ever-increasing mobile data traffic and network costs. C-RAN is a practical solution to the strict energy-constrained wireless sensor nodes, often found in Internet of Things (IoT) applications. Although this architecture can provide energy efficiency and reduce cost, it is a challenging task in C-RAN to utilize the resources efficiently, considering the dynamic real-time environment. Several research works have proposed different methodologies for effective resource management in C-RAN. This study performs a comprehensive survey on the state-of-the-art resource management techniques that have been proposed recently for this architecture. The resource management techniques are categorized into computational resource management (CRM) and radio resource management (RRM) techniques. Then both of the techniques are further classified and analyzed based on the strategies used in the studies. Remote radio head (RRH) clustering schemes used in CRM techniques are discussed extensively. In this research work, the investigated performance metrics and their validation techniques are critically analyzed. Moreover, other important challenges and open research issues for efficient resource management in C-RAN are highlighted to provide future research direction.
The Root Foraging Response under Low Nitrogen Depends on DWARF1-Mediated Brassinosteroid Biosynthesis1[OPEN]

Mild nitrogen deficiency upregulates key genes involved in brassinosteroid biosynthesis to stimulate root elongation. Root developmental plasticity enables plants to adapt to limiting or fluctuating nutrient conditions in the soil. When grown under nitrogen (N) deficiency, plants develop a more exploratory root system by increasing primary and lateral root length. However, mechanisms underlying this so-called foraging response remain poorly understood. We performed a genome-wide association study in Arabidopsis (Arabidopsis thaliana) and we show here that noncoding variations of the brassinosteroid (BR) biosynthesis gene DWARF1 (DWF1) lead to variation of the DWF1 transcript level that contributes to natural variation of root elongation under low N. In addition to DWF1, other central BR biosynthesis genes upregulated under low N include CONSTITUTIVE PHOTOMORPHOGENIC DWARF, DWF4, and BRASSINOSTEROID-6-OXIDASE2. Phenotypic characterization of knockout and knockdown mutants of these genes showed significant reduction of their root elongation response to low N, suggesting a systemic stimulation of BR biosynthesis to promote root elongation. Moreover, we show that low N-induced root elongation is associated with aboveground N content and that overexpression of DWF1 significantly improves plant growth and overall N accumulation. Our study reveals that mild N deficiency induces key genes in BR biosynthesis and that natural variation in BR synthesis contributes to the root foraging response, complementing the impact of enhanced BR signaling observed recently. Furthermore, these results suggest a considerable potential of BR biosynthesis to genetically engineer plants with improved N uptake.
Investigation of the Dynamism of Nanosized SOA Particle Formation in Indoor Air by a Scanning Mobility Particle Sizer and Proton-Transfer-Reaction Mass Spectrometry

Terpenes are VOCs of particular importance, since they are emitted from a wide range of indoor sources and are considered to be precursors of Secondary Organic Aerosol (SOA) formation. It has been proven that SOA particles, especially nanosized ones, pose a threat to human health. In this research, experiments with the application of an environmental chamber and real-time measurement techniques were carried out to investigate in a complimentary way the formation of monoterpene oxidation products and nanosized SOA particles initiated by monoterpene ozonolysis. Proton-Transfer-Reaction Mass Spectrometry with a Time-Of-Flight analyzer (PTR-TOF-MS) and a Scanning Mobility Particle Sizer (SMPS) were applied to determine in real time the dynamism of the formation of the corresponding terpene ozonolysis products and submicron SOA particles. Results proved that firstly, oxidation products were formed, and then, they underwent nucleation and condensation, forming particles whose diameters grew with time. The oxidation products formed were different depending on the type of terpenes applied. The comparison of the results obtained during the experiments with gaseous standard mixtures and real samples commonly present and used in indoor air revealed that the diversified chemical composition of the emission source had implications for both the particle formation initiated by the oxidation of essential oil components and the chemical reactions occurring via the oxidation process. With the instrumentation utilized, the concentration changes at the level of a few ppbv could be monitored.
Risk factors and etiology of childhood hearing loss: a cohort review of 296 subjects

Abstract Background: The association between the Joint Committee on Infant Hearing (JCIH) risk factors and etiology of hearing loss (HL) is not studied well in children. Objectives: To clarify the etiologic causes and evaluate the JCIH risk characteristics of children with HL. Methods: A retrospective study of 296 children with HL born between 2009.01 and 2013.12 in Stokholm. Demographic data, family and medical histories, audiologic results, imaging findings, and genetic results were ascertained and analyzed. Results: In 221 with bilateral hearing loss (BHL), family history and neonatal risk indicators were the most common risks (59 each), followed by syndrome related risks. In 75 with unilateral hearing loss (UHL), craniofacial anomaly was the most common risk, followed by family history. Etiology was established in 93 with BHL, in which syndromic HL accounted for 37.2%, chromosomal aberrations for 21.3%, and environmental causes for 19.1%. Etiology was established in 35 with UHL, in which ear malformation accounted for the most (74.3%), followed by environmental causes (14.3%). Conclusions and significance: Childhood HL can be attributed to a variety of causes with an etiology identifiable in 42.5% of BHL and 46.7% of UHL. BHL and UHL have different patterns of JCIH risk exposure and etiology.
Inhibition of Acyl-CoA Elongation by Chloroacetamide Herbicides in Microsomes from Leek Seedlings

Abstract The inhibition of very-long-chain fatty acid (VLCFA) synthesis by chloroacetamide herbicides was studied in vivo as well as in vitro . Incorporation of [2- 14 C]malonate into C 20 to C 24 VLCFAs of etiolated leek seedlings was strongly inhibited by the chloroacetamide metazachlor (I 50 ≈ 10–100 nM). The I 50 values of inhibition of incorporation into each VLCFA decreased with their chain length [I 50 (20:0) ≈ 1 μM, I 50 (22:0) ≈ 100 nM, I 50 (24:0) ≈ 10 nM]. With a microsomal preparation from leek seedlings an in vitro elongase assay to quantify chloroacetamide activity was developed using [2- 14 C]malonyl-CoA and acyl-CoA (16:0-CoA to 22:0-CoA) as substrates. Under our assay conditions the I 50 values of inhibition by metazachlor of each step of acyl-CoA elongation were determined to be 0.5 μM (for elongation of 16:0-CoA), 1.7 μM (18:0-CoA), 0.7 μM (20:0-CoA), and 0.5 μM (22:0-CoA). Furthermore, it could be shown that inhibition increased with decreasing concentrations of acyl-CoA primer substrate. Using the chiral chloroacetamide metolachlor acyl-CoA elongation was only inhibited with the herbicidally active S -enantiomer while the R -enantiomer had no influence. As demonstrated by 20:0-CoA elongation the I 50 value decreased about 10-fold after preincubation of the enzyme preparation at higher temperatures. Furthermore, enzyme activity could not be recovered by dilution of the enzyme–inhibitor complex. These findings strongly support the hypothesis that a covalent binding of metazachlor to its target may occur. From this study the mode of action of herbicidal chloroacetamides is disclosed as a strong inhibition of VLCFA synthesis resulting from (a) an inhibition of each elongation step in series, (b) the increase of that inhibition with the decrease of acyl-CoA primer substrate concentration, and (c) the tight binding of the inhibitor to the target enzyme.
A Novel and Simpler Alkaline Hydrolysis Methodology for Extraction of Ferulic Acid from Brewer’s Spent Grain and its (Partial) Purification through Adsorption in a Synthetic Resin

This work aims to develop simpler methodologies of extracting ferulic acid (FA) from brewer’s spent grain (BSG). BSG is produced by brewing companies at high amounts all over the year and does not possess a direct application. Thus, its use as raw material for extraction of bioactive compounds has gained attention in the last years. FA has different interesting applications in cosmetics, food industry, and pharmaceutics. Several studies aim for its extraction from BSG by various methods, namely alkaline hydrolysis. In the present work, we suggest the use of autoclave to process higher amounts of BSG in a lab scale. A simplification of the regular post-hydrolysis procedures is also proposed to decrease the number of experimental steps and energy costs and to simultaneously increase the extraction yield (up to 470 mg of FA per 100 g of BSG). The adsorption of extracted FA in a synthetic resin is suggested as a partial purification method.
Interleukin‐6 Protects MIN6 β Cells from Cytokine‐Induced Apoptosis

Abstract: Host nonspecific β cell injury by cytokines has been implicated in the process of early islet graft dysfunction. Islet transplant destruction by cytokines released from inflammatory cells that infiltrate the graft is thought to be mainly mediated by NF‐κB‐dependent nitric oxide (NO) production by the islet. In this study, we aimed to evaluate the role of IL‐6 in making a β cell resistant to cytokine‐induced apoptosis by decreasing the NF‐κB‐dependent NO production and compared the result with that of the expression of a dominant negative inhibitor of NF‐κB (DN NF‐κB). Incubation of MIN6 cells with IL‐1β, IFN‐γ, and TNF‐α (cytomix) increased production of nitrites, with increased expression of iNOS mRNA. When treated with cytomix, the DN NF‐κB‐transfected mutant demonstrated significantly less nitrite production and apoptosis than parent MIN6. NO production was effectively blocked by IL‐6 as well as by N‐monomethyl‐l‐arginine (l‐NMMA). Inhibition of the NO production led to decreased rate of apoptosis accompanied by downregulation of the proapoptotic molecule Bax and increased expression of the antiapoptotic molecule Bcl‐2 and Bcl‐xL. These data indicate that cytokine‐induced cell death in the MIN6 β cell line involves mechanisms that are, in part, NF‐κB‐ and NO‐dependent. Inhibition of the NO production by the incubation of the MIN6 cells by the pretreatment of IL‐6 or l‐NMMA is cytoprotective and can be used as a substitute for the expression of DN NF‐κB.
Role of Crop Residue Management in Sustainable Agricultural Development in the North China Plain

ABSTRACT Crop residue, the largest product of agricultural harvests, contains large amounts of assimilated carbon (C) and nutrients such as nitrogen (N), phosphorus (P), and potassium (P); these elements must be recycled for the sustainable development of agriculture. Crop residue management should serve a double function, both confronting global warming and food security by increasing carbon sequestration in agriculture and increasing grain yields. Historically, the North China Plain has experienced different crop residue management practices. While direct burning in the field remains an environmental problem in the region, crop residue amendment triggers benign cycling of C and nutrients in agriculture. Data showed that soil organic carbon (SOC) in topsoil increased by 0.174 to 1.74 g kg−1, with an average of 0.79 g kg−1 after wheat residue amendment collected from 35 sites in the North China Plain. The average increase in grain yield achieved by wheat residue amendment in the region is 260 kg ha−1 year−1 for wheat and 310 kg ha−1 year−1 for maize, for a total of 570 kg ha−1 year−1. At the same time, the available potassium (K2O), the available phosphorus (P2O5), and total nitrogen in soil increased, significantly or not. With the development of the economy, technology, and supporting policy, crop residue management can play an increasingly important role in sustainable agricultural development.