Miracle-dz/UniFAR-FTUs huggingface dataset:
Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)

The research include studying biochemical and genetic molecular study for paraoxonase1 enzyme and identify some biochemical parameters for serum blood samples 68 women with breast cancer and follow-up after take them three doses of chemotherapy and comparative with 50 normal female as control group.The result showed for women with breast cancer a significant increase in (TC,MDA ,Total protein, U,A , calcium and BMI) when compared with normal women, the results also showed for women who have taken chemical doses and women with breast cancer significant decreased in (PON1, GSH) compared with normal women, the results showed for women who have taken the first dose significant increase in (MDA,TP, Calcium, U.A , BMI), while second dose and the third dose chemotherapy found significant increase in (TP, U.A ,Calcium ) and significant decreased for (PON1). The results included sequential analysis for PON1 enzyme ,it has shown results for genetic analyzed selected exon (Exon4), where DNA extraction for normal women and breast cancer women and taken their doses of chemotherapy and measure DNA concentration and purity using Nano-Drop Spectrophotometric Analysis where the result show the DNA concentration ranged between (5.2-68.4) ng/ml where concentration mean for women with breast cancer (26.97) ng/ml and (17.49) ng/ml for normal women while the first dose 24.74 ng/ml , (16.16-9.70)ng/ml for second and third dose respectively . It has also been used PCR technique amplification and test products PCR analysis of sequential technique by using gel electrophoresis where packet appeared clear without deformation and tested PCR product sequencing for exon4 by capillary automated sequencer and comparing the result with the normal sequence of the oxon. , the results shows for women have taken first dose deletion mutation called (Remove) frame shift mutation clear nitrogen base one lead to change all codons after deletion site so that all amino acid change in poly protien chain which led to the loss of amino acid (Tyr.) . and mutation type of silent mutation third mutation type of substitution mutation (Replace amino acid (Thr.) by amino acid (Ser.).
The Daisyworld control system

The original Gaia Hypothesis proposed that life on Earth, along with the oceans, atmosphere and crust, forms a homeostatic system which reduces the effects of external perturbations, so that conditions are maintained to within the range that allows widespread life. Daisyworld is a simple mathematical model intended to demonstrate certain aspects of this planetary homeostasis. There have been a considerable number of extensions and developments to the original Daisyworld model. Some of this work has been produced in response to criticism of the Gaia Hypothesis and Daisyworld specifically and some has been produced by using Daisyworld as a testbed to explore a range of issues. This thesis examines the Daisyworld control system and in doing so explains how Daisyworld performs homeostasis. The control system is classified as a rein control system which is potentially applicable to a wide range of scenarios from physiological and environmental homeostasis to robotic control. A series of simple Daisyworld models are produced and aspects of the original Daisyworld are explained, in particular the inverse response to forcing: why temperature goes down on Daisyworld when the brightness of the star increases. The Daisyworld control system is evaluated within an evolutionary context. A key result is that environmental regulation emerges not despite of Darwinian evolution but because of it. Within an ecological context, it is found that increasing the complexity of a self-regulating ecosystem can increase its stability. An energy balance climate model is developed to assess the effects of non-equilibrium thermodynamic processes on the Daisyworld control system. Results are presented that support the hypothesis that when the system is in a state of maximum entropy production, homeostasis is maximised.
Microbial population changes during sourdough fermentation monitored by DGGE analysis of 16S and 26S rRNA gene fragments

In this study, the microbial population dynamics were monitored during a tra- ditional sourdough fermentation process using an integrated approach, including PCR- DGGE and conventional culturing procedures. Total DNA was extracted directly from samples taken at intervals during 24 h of fermentation using a rapid protocol. Partial 16S and 26S rDNA sequences from bacteria and yeasts, respectively, were amplified using two new pairs of primers and the products obtained were separated using denaturing gra- dient gel electrophoresis (DGGE). DGGE profiles indicated changes in the lactic acid bac- teria (LAB) population structure. Sequencing of purified rDNA amplicons revealed that Lactobacillus sanfranciscensis, Lactobacillus brevis, Lactobacillus arizonensis-Lacto- bacillus plantarum group and Lactobacillus kimchii-Lactobacillus paralimentarius pre- vailed throughout fermentation. A close relative of the strain CS1, proposed to be a new species associated with Italian sourdoughs, was also detected. The yeast population was represented only by Saccharomyces cerevisiae species. Conventional culture-dependent methods partially reflected PCR-DGGE results, as a lower diversity in LAB species was detected. The present approach proved to be effective in giving a reliable overview of the microbial components of sourdough in a short time.
Accessibility and safety of antipsychotics in the treatment of autism spectrum disorder in children and adolescents

 ASD-QIND-MT measured the influence of screening tools; Indian Scale of Assessment of Autism (ISAA) (India) and Childhood Autism Rating Scale (CARS) (Malta) on the prescribing behaviour of RiAr.  Comparison of the price of RiAr in India and Malta was undertaken to determine accessibility to RiAr.  Eudravigilance signal detection and assessment to detect potential ADRs were conducted to be included in the SmPC of RiAr (Figure 1).
Evaluation of Alternaria alternata (Fr.) Keissler for biological control of Trianthema portulacastrum L.

Trianthema portulacastrum is an obnoxious annual weed, which causes substantial yield reduction on account of competition in several cultivated crops. A foliar pathogen Alternaria alternata was evaluated as a potential biological control agent for effective management of T. portulacastrum with an aim to find the most virulent strain and to evaluate and standardize its application method. The pathogenicity of A. alternata increased with increasing inoculum density, and spore concentration of 107 & 108 spores ml- 1 caused 100 per cent mortality of 10 to 30 days old T. portulacastrum that were raised from the seeds, and also of the naturally grown plants having length of 2-25 cm and 6-182 number of leaves.
Riverscape-mediated effects of introduced trout on non-diadromous galaxiid fishes in New Zealand

1 Chapter 1: General introduction 6 Chapter 2: Evidence of source-sink metapopulations in a vunerable native 14 galaxiid fish driven by introduced trout Chapter 3: Biotic and abiotic drivers of co-occurrence between introduced 38 trout and native alpine galaxias (Galaxias paucispondylus) across New Zealand riverscapes Chapter 4: Location of demographic sources affects distributions of a 62 vulnerable native fish in invaded river networks Chapter 5: Development of a spatial model predicting exclusion of a 84 vulnerable native fish by introduced trout based on landscape features Chapter 6: Conserving non-diadromous galaxiids across trout-invaded 106 riverscapes
The Effectiveness of using oranges to delay fatigue appearance and improve the skill level of swimming specialized students

Swimming is considered one of the types of water sports that have a great importance, where scientists, doctors and sports leaders agreed that swimming is an outstanding sport. This prestigious status is due to the various physical, psychological and social values that its practitioners gains. Sports physiologists’ shows great interest in swimming, in order to study the training methods that can be used to achieve the physiological responses necessary to adapt with the exerted effort, increase the body’s functional efficiency and improve the metabolism to achieve the best results. Swimming requires proper nutrition to ensure energy production, meet dietary needs and allow muscles recovery between and during performing exercises. The recovery by nutrition works by restoring tissues and compensating fuel by compensating muscle glycogen and protein (1: 6, 187) (8: 166)
Pulse mode irradiation at Radachlorin PDT shifted cell death to apoptosis in vitro

Photodynamic therapy (PDT) is a clinically approved treatment that can exhibit onsite cytotoxic activity toward tumor cells. One of the main factors limiting PDT efficiency is tissue hypoxia derived from photodynamic action. PDT with pulse mode irradiation at the same peak fluence rates as in continuous wave (CW) mode and with appropriate irradiation parameters could be more effective in the potency of 1O2 generation and the cytotoxic effect enhancement by tissue reoxygenation. In this study, we demonstrated theoretically that the main parameter of pulse mode irradiation is the intermittency factor, which makes it possible to maintain the intended 3O2 concentration and to regulate the efficiency of 1O2 generation. We also showed experimentally that photodynamic treatment with pulse mode irradiation has congruent cytotoxicity to CW mode but induces preferable cell apoptosis. We assume that not only is cumulative 1O2 concentration is important in photodynamic cytotoxicity, but so is the temporal distribution of 1O2 generation, which determines the types of cell death.
Accumulation of Mutant α1-Antitrypsin Z in the Endoplasmic Reticulum Activates Caspases-4 and -12, NFκB, and BAP31 but Not the Unfolded Protein Response*

In α1-antitrypsin (α1AT) deficiency, a polymerogenic mutant form of the secretory glycoprotein α1AT, α1ATZ, is retained in the endoplasmic reticulum (ER) of liver cells. It is not yet known how this results in liver injury in a subgroup of deficient individuals and how the remainder of deficient individuals escapes liver disease. One possible explanation is that the “susceptible” subgroup is unable to mount the appropriate protective cellular responses. Here we examined the effect of mutant α1ATZ on several potential protective signaling pathways by using cell lines with inducible expression of mutant α1AT as well as liver from transgenic mice with liver-specific inducible expression of mutant α1AT. The results show that ER retention of polymerogenic mutant α1ATZ does not result in an unfolded protein response (UPR). The UPR can be induced in the presence of α1ATZ by tunicamycin excluding the possibility that the pathway has been disabled. In striking contrast, ER retention of nonpolymerogenic α1AT mutants does induce the UPR. These results indicate that the machinery responsible for activation of the UPR can distinguish the physical characteristics of proteins that accumulate in the ER in such a way that it can respond to misfolded but not relatively ordered polymeric structures. Accumulation of mutant α1ATZ does activate specific signaling pathways, including caspase-12 in mouse, caspase-4 in human, NFκB, and BAP31, a profile that was distinct from that activated by nonpolymerogenic α1AT mutants.
Modelo para conversão de texto em hipertexto semântico no domínio temático da intensificação agropecuária.

Resumo: Descreve os resultados de uma pesquisa de mestrado que teve como objetivo propor um modelo para conversao de texto linear em hipertexto com abordagem semântica, cujas relacoes expressas nos links propiciem a aquisicao de conhecimento. No contexto de aplicacao desta pesquisa, o dominio agropecuario, o modelo foi aplicado na teoria da Intensificacao Agropecuaria, cujo teor teorico-conceitual esta apresentado no livro de Ester Boserup (1965). Os elementos teorico-metodologicos analisados foram os fundamentos dos Sistemas de Organizacao do Conhecimento, a Teoria da Classificacao Facetada, a Teoria do Conceito, principios provenientes da Linguistica e da Terminologia e aportes da Teoria da Aprendizagem Significativa. Alem disso, foram abordados os principais estudos mapeados na literatura sobre a conversao de texto linear em hipertexto, incluindo os campos da Ciencia da Computacao, Linguistica, Educacao, com enfase na Biblioteconomia e na Ciencia da Informacao. Partiu-se do pressuposto de que esses estudos nao deixaram explicitos todos os processos necessarios a conversao. Os procedimentos metodologicos sao de abordagem qualitativa e exploratoria, caracterizando-se como um estudo de caso de natureza aplicada no dominio agropecuario. Os resultados sugerem que o modelo, ao produzir criterios consistentes para trabalhar os aspectos conceituais e semânticos de um dominio, oferece suporte a conversao de um texto linear em hipertexto semanticamente enriquecido. Assim, conclui-se que, ao construir uma estrutura hipertextual que apresenta a informacao de forma organizada e semelhante a estrutura cognitiva do leitor, e possivel que ocorra a aquisicao de conhecimentos mais significativos do que se comparado a disponibilizacao de informacoes apenas no formato linear.
Epizootic ulcerative syndrome (EUS) in bata, Labeo bata (Hamilton 1822) from Tripura, India

The occurrence of epizootic ulcerative syndrome (EUS) in bata ( Labeo bata ) and the infectivity and role of Aphanomyces invadans in the aetiology of ulcerative condition were investigated in the present study. Live fish ( L.bata ) samples with ulcerations were collected from a local fish farm in Tripura and were acclimatised in laboratory tanks with proper management. Case histories, clinical and gross signs of the sampled fishes were recorded. Histopathological study of muscle and liver tissue from the ulcerated fish showed the presence of oomycete hyphae and granulomas. Oomycete isolation was done from the affected muscles and was identified as Aphanomyces invadans based on colony morphology, growth and microscopic morphology. The results suggest that the ulcerative condition in bata was due to EUS associated with A. invadans .
Emission modelling of hydrodynamic AGN jet simulations

Abstract Radio-loud Active Galactic Nuclei (AGN) produce relativistic jets that can be modelled with relativistic hydrodynamic (RHD) simulations. In this study we present two such simulations of jets, used to investigate the parameters required to reproduce structures consistent with both FR I and FRII jets. In the first simulation a Lorentz factor of 10 and supersonic flow of Mach 30 were chosen, while for the second simulation a Lorentz factor of 1.0014 with a supersonic flow of Mach 4 was used. Over similar distances scales the first case shows a well collimated beam with a strong shock at the interface between the jet and ambient medium while the second case shows a less stable beam and a larger cocoon. To determine whether the simulated physical structures are consistent with the observed FR I/II jets, the synchrotron emission has been calculated to produce radio maps at a single frequency of 1.5 GHz.
Estudo comparativo dos metabolitos do exemestano num modelo celular de cancro da mama estrogénio-dependente

ix Indice xi Lista de imagens xiii Lista de tabelas xiv Lista de abreviaturas xv Capitulo I: Introducao 3 1.1. Cancro da mama estrogenio-dependente 5 1.1.1. Estrogenios 6 1.1.2. Recetores de estrogenio 6 1.1.3. Vias de sinalizacao 8 1.1.4. Sintese de estrogenios 11 1.1. 5. Aromatase 13 1.2. Terapia endocrina 15 1.2. 1. Modeladores e inativadores dos recetores de estrogenio 15 1.2. 2. Inibidores da aromatase 16 1.3. Exemestano 18 1.3. 1. Farmacocinetica e farmacodinâmica 21 1.3. 2. Metabolitos do exemestano 22 1.3. 3. Efeitos biologicos 24 1.3. 4. Eficacia terapeutica 25 1.3. 5. Seguranca e efeitos secundarios 28 1.4. Resistencia a terapia endocrina 31 1.4. 1. ER e os co-reguladores 31 1.4. 2. Crosstalk entre ER e recetores de tirosina cinases (RTK) 35
Inter-annual and body topographic consistency in the plumage bacterial load of Great tits

The Estonian Science Foundation (grant number ETF8566 to R.M. and ETF8376 to V.T.), the Estonian Ministry of Education and Science (target-financing project number SF0180004s09) and the European Regional Development Fund (Center of Excellence FIBIR).
Regulaçăo do metabolismo de nitrogęnio em Azospirillum brasilense

XIV 1. INTRODUÇÃO . 1 1.1 O gênero Azospirillum. 1 1.2 A fixação biológica de nitrogênio. 2 1.3 Nitrogenase . 4 1.4 Assimilação de amônio . 6 1.5 Proteínas da família PII. 7 1.6 Estrutura das proteínas da família PII. 7 1.7 Sistema Ntr. 8 1.8 Proteína GlnK . 11 1.9 Formação de heterotrímeros . 12 1.10 Moléculas efetoras das proteínas PII . 13 1.11 Transportadores de amônio. 14 1.12 Família Amt . 15 1.13 O gene amtB em A. brasilense . 16 1.14 Substrato e estrutura das proteínas Amt. 16 1.15 Complexo GlnK-AmtB. 18 1.16 Proteínas tipo PII em A. brasilense. 20 1.16. 1 A proteína GlnB. 20 1.16. 2 A proteína GlnZ. 21 1.17 Modificação pós-traducional de GlnB e GlnZ. 22 1.18 O gene glnD em A. brasilense . 22 1.19 Sistema NtrY-NtrX . 23 1.20 Organização estrutural de promotores em procariotos . 25 1.20. 1 Promotores do tipo σ . 26 1.20. 2 Promotores do tipo σ . 26 1.21 Regulação transcricional por NtrC. 27 1.22 Regulação transcricional da nitrogenase. 28 1.22. 1 A Proteína NifA. 28 1.22. 2 Regulação da atividade da proteína NifA. 29 1.23 Regulação pós-traducional da nitrogenase. 30 1.23. 1 As proteínas DraT e DraG. 32 1.23. 2 Controle do desligamento/religamento da nitrogenase por ADP-ribosilação . 33 1.23. 3 Papel de proteínas PII e Amt no controle do desligamento/religamento da nitrogenase por ADP-ribosilação. 34 1.23. 4 Regulação da atividade de DraT e DraG pelo estado de oxi-redução da proteína NifH . 37
Characterization of P-glycoprotein Expression as a Multixenobiotic Resistance Mechanism in Fish

Abstract : P-glycoproteins, responsible for multidrug resistance, function as energy dependent efflux flippases that prevent the cellular accumulation of moderately hydrophobic chemicals. We characterized P-gp expression in populations of several fish species exposed in their natural habitat to environmental contaminants which may be P-gp substrates/inducers. We evaluated whether P-gp activity may be implicated in this multixenobiotic resistant phenotype. P-glycoprotein levels were elevated in tissue surrounding liver tumors in winter flounder (Pleuronectes americanus). Killifish (Fundulus heteroclitus) from a contaminated field sites had higher intestinal P-gp and lower hepatic P-gp than control killifish. P-glycoprotein was induced in fish by oil exposure but not by Beta-naphthoflavone or 2,3,7,8-tetrachlorodibenzofuran. Thus, P-gp expression is not regulated by the aryl hydrocarbon receptor pathway. We developed a protocol for an in vivo assay to simultaneously evaluate P-gp-mediated transport of a model substrate, rhodamine B (rhB), in multiple organs of killifish. Our results indicate that P-gps play a major role in transport of xenobiotics in fish especially in liver, brain, and ovary. Using this assay, we assessed whether the common environmental contaminant and carcinogen benzoApyrene (BaP) is a P-gp substrate. We show that BaP and/or its CYP1A metabolites are not transported by P-gp in liver, brain, or ovary.
APPLICATION OF GIS TECHNIQUESIN EVALUATION OF URBAN SPRAWL

Sprawl is basically spreading of city. Urban Sprawl is the outward spreading of urban areas and suburbs around outskirts of the city.Urban land-use advancements of various kinds greatly affect the landscape. Such advancement cause change the function of urban landscape and it also leads to change in ecosystem services. In this paper, an attempt has been made to identify and analyse the rate of urban growth, characteristics of urban sprawl and to investigate the effect of Urban Sprawl on Land use / Land cover change of Lucknow City for year 1981-2016. Remote sensing and GIS is used to analyse the urban sprawl mapping and detect changes of sprawl of Lucknow city through different year. Satellite data are found to be very useful in mapping and quantifying the land use/land cover change in different time periods through Landsat images and GIS.
Retrospektive Analyse des Therapieverlaufs und kardiovaskulären Risikoprofils bei Patienten mit Rheumatoider Arthritis unter immunsuppressiver Therapie

Objective: Previous studies have shown that the cardiovascular risk in patients with rheumatoid arthritis (RA) is higher than among the general population. This study assesses the prevalence of cardiovascular events such as myocardial infarction and stroke in RA patients treated with a DMARD or a biologic. In contrast to many studies that use a preselected group of patients this study specifically aims to reflect the typical RA patient with a variety of comorbidities and comedication. Methods: In this retrospective analysis data from 275 patients who received immunosuppressive RA treatment between April 1994 and August 2016 were evaluated for disease progression, comorbidities, cardiovascular risk factors and scores and occurrence of cardiovascular events. Subgroups were formed according to the leading basic medication. This involved the use of the classic DMARDs methotrexate and leflunomide and the biologics adalimumab, etanercept, rituximab and tocilizumab. Results: All six basic therapeutics studied achieved a significant reduction in DAS28 (average baseline 4.67) by about one point after 6 months (3.65) and towards the end of observation (3.78). This trend was reflected in the analysis of the erythrocyte sedimentation rate (20.1; 13.1; 15.6 [mm/1h]) and the C-reactive protein level (11.1; 5.7; 6.9 [mg/l]). All three calculated scores for the evaluation of cardiovascular risk increased during the course of the study. The Framingham score rose by 0.9 points, the PROCAM score for calculating the risk of myocardial infarction by 0.4 points. The risk of stroke increased by 0.5 points according to the PROCAM score. During the observation period 13 cardiovascular events occurred, eight of them related to a myocardial infarction, five to a stroke. Discussion and conclusion: The analysis of rheumatological parameters can prove good efficacy for all six drugs studied. The result of cardiovascular risk calculation is in contrast to this. According to the results of previous studies an optimized cardiovascular risk profile could be expected from the successful treatment of the underlying rheumatic disease. However, the present work cannot prove this tendency. It must be questioned whether the current score systems for the evaluation of cardiovascular risk in RA patients are suitable. Ultimately, none of them are directly correlated with disease activity. Further investigations could be carried out at this point.