Miracle-dz/UniFAR-FTUs huggingface dataset:
Framework for the Automation of Business Processes

Mangkubumi Yogyakarta area is not only a strategic area in the city of Yogyakarta, which lies on the axis of philosophy, forming the spatial structure of the city of Yogyakarta but also a compact area with building facilities that function as offices, education, trade/services, worship, and settlements. The discourse of developing the site has long been announced as a supporting area for Malioboro tourism, and Tugu station developed into TOD (Transit Oriented Development), among others. In 2019, the research, development research aiming to find an area model supporting the realization of the maximum mobility value was carried out in Mangkubumi area to measure mobility levels in the area, and the results of this study found that the rate of mobility and walkability was 76 and 79 respectively. The method used was a simulation of the Urban Modeling Interface (UMI) software to measure the value of mobility in regional models. The research step used area modeling, which was carried out in eight models. After the simulation, the best model with the highest mobility value was selected. The eight models were made based on the four road network patterns of Morlok’s idea (Grid, Spiral, Hexagonal, and Delta), each of which was designed to be two types, namely, type A and type B. Type A was a form of modeling by conducting interventions focused on residential building facilities, and type B was focused on all area building facilities. The finding of this research was that the best road network pattern was the Delta network pattern after intervening in all building facilities and after carrying out land use by placing residential building facilities at the center of the area and placing other building facilities on edge the area. This simulation resulted in a walkability score of 92 and bike-ability of 92. The score is the highest in the walk score ranking level. A value of 92 means that there are walker's paradise, daily business not requiring a car, and biker's paradise, daily affairs done by cycling.
Risk for Irritable Bowel Syndrome in Patients with Helicobacter Pylori Infection: A Nationwide Population-Based Study Cohort Study in Taiwan

Background: The association between Helicobacter pylori (H. pylori) infection and the risk of developing irritable bowel syndrome (IBS) has yet to be investigated; thus, we conducted this nationwide cohort study to examine the association in patients from Taiwan. Methods: A total of approximately 2669 individuals with newly diagnosed H. pylori infection and 10,676 age- and sex-matched patients without a diagnosis of H. pylori infection from 2000 to 2013 were identified from Taiwan’s National Health Insurance Research Database. The Kaplan–Meier method was used to determine the cumulative incidence of H. pylori infection in each cohort. Whether the patient underwent H. pylori eradication therapy was also determined. Results: The cumulative incidence of IBS was higher in the H. pylori-infected cohort than in the comparison cohort (log-rank test, p < 0.001). After adjustment for potential confounders, H. pylori infection was associated with a significantly increased risk of IBS (adjusted hazard ratio (aHR) 3.108, p < 0.001). In addition, the H. pylori-infected cohort who did not receive eradication therapy had a higher risk of IBS than the non-H. pylori-infected cohort (adjusted HR 4.16, p < 0.001). The H. pylori-infected cohort who received eradication therapy had a lower risk of IBS than the comparison cohort (adjusted HR 0.464, p = 0.037). Conclusions: Based on a retrospective follow-up, nationwide study in Taiwan, H. pylori infection was associated with an increased risk of IBS; however, aggressive H. pylori infection eradication therapy can also reduce the risk of IBS. Further underlying biological mechanistic research is needed.
Artificial Small RNA-Based Silencing Tools for Antiviral Resistance in Plants

Artificial small RNAs (art-sRNAs), such as artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs), are highly specific 21-nucleotide small RNAs designed to recognize and silence complementary target RNAs. Art-sRNAs are extensively used in gene function studies or for improving crops, particularly to protect plants against viruses. Typically, antiviral art-sRNAs are computationally designed to target one or multiple sites in viral RNAs with high specificity, and art-sRNA constructs are generated and introduced into plants that are subsequently challenged with the target virus(es). Numerous studies have reported the successful application of art-sRNAs to induce resistance against a large number of RNA and DNA viruses in model and crop species. However, the application of art-sRNAs as an antiviral tool has limitations, such as the difficulty to predict the efficacy of a particular art-sRNA or the emergence of virus variants with mutated target sites escaping to art-sRNA-mediated degradation. Here, we review the different classes, features, and uses of art-sRNA-based tools to induce antiviral resistance in plants. We also provide strategies for the rational design of antiviral art-sRNAs and discuss the latest advances in developing art-sRNA-based methodologies for enhanced resistance to plant viruses.
Aptamer-based lab-on-chip for cancer cell isolation and detection

The isolation and detection of circulating tumor cells (CTCs), when these are few in number or small in mass, can enable early cancer detection [1]. Currently, most research endeavors to isolate circulating tumor cells rely on antibodies and immunohistochemistry [2,3]. Aptamer are alternative molecules that have affinities and specificities that are comparable to those of antibodies [4,5], but these can be more readily adapted to many applications [6–9], including lab-on-a-chip devices. Here, we report use of a novel epidermal growth factor receptor (EGFR) aptamer biochip to identify and isolate cancer cells that overexpress EGFR. These results provide a solid basis for the development of diagnostic devices that can readily identify and isolate CTCs.Copyright © 2010 by ASME
Bacillus cytotoxicus Isolated from a Pristine Natural Geothermal Area Reveals High Keratinolytic Activity

Geothermal areas are the niches of a rich microbial diversity that is not only part of the intangible patrimony of a country but also the source of many microbial species with potential biotechnological applications. Particularly, microbial species in geothermal areas in Argentina have been scarcely explored regarding their possible biotechnological uses. The purpose of this work was to explore the proteolytic and keratinolytic enzymatic potential of microorganisms that inhabit in the Domuyo geothermal area in the Neuquén Province. To this end, we did enrichment cultures from two high-temperature natural samples in mineral media only supplemented with whole chicken feathers. After the isolation and the phylogenetic and morphologic characterization of different colonies, we obtained a collection of Bacillus cytotoxicus isolates, a species with no previous report of keratinolytic activity and only reported in rehydrated meals connected with food poisoning outbreaks. Its natural habitat has been unknown up to now. We characterized the proteolytic and keratinolytic capacities of the B. cytotoxicus isolates in different conditions, which proved to be remarkably high compared with those of other similar species. Thus, our work represents the first report of the isolation as well as the keratinolytic capacity characterization of strains of B. cytotixicus obtained from a natural environment.
Cytogenetical Study of Rubus×tawadanus (Rosaceae)

Rubus×tawadanus (2n=21) has been reported to be a hybrid between R. parvifolius (2n=14) and R. sieboldii (2n=28) (Migo 1970, Hatusima 1971). In this study, karyotypic comparisons among R. ×tawadanus, R. parvifolius and R. sieboldii indicated that the 3x chromosome complement of R. ×tawadanus was composed of one basic set of R. parvifolius and two from R. sieboldii. Hence the taxonomic treatment of R. ×tawadanus was supported by the study. The lack of chromosome pairing in PMCs of R. ×tawadanus showed that this hybrid has 3 non-homologous chromosome sets. This indicates that there is no genomic homology among either the chromosomes of R. parvifolius and the two genomes of R. sieboldii nor between the 2 basic sets within R. sieboldii. For this reason, R. sieboldii is thought to be allotetraploid.
Lipid–Protein and Protein–Protein Interactions in the Pulmonary Surfactant System and Their Role in Lung Homeostasis

Pulmonary surfactant is a lipid/protein complex synthesized by the alveolar epithelium and secreted into the airspaces, where it coats and protects the large respiratory air–liquid interface. Surfactant, assembled as a complex network of membranous structures, integrates elements in charge of reducing surface tension to a minimum along the breathing cycle, thus maintaining a large surface open to gas exchange and also protecting the lung and the body from the entrance of a myriad of potentially pathogenic entities. Different molecules in the surfactant establish a multivalent crosstalk with the epithelium, the immune system and the lung microbiota, constituting a crucial platform to sustain homeostasis, under health and disease. This review summarizes some of the most important molecules and interactions within lung surfactant and how multiple lipid–protein and protein–protein interactions contribute to the proper maintenance of an operative respiratory surface.
Development of an integrated pest management programme for cotton. Part 2: Integration of a lucerne/cotton interplant system, food supplement sprays with biological and synthetic insecticides

Part 1 of the paper showed that the integration of a lucerne/ cotton interplant system with supplementary food sprays retained and increased populations of predatory insects in the cotton crop. The strategic use of biopesticides (Bacillus thuringiensis (Bt), nuclear polyhedrosis virus (NPV)) reduced the use of synthetic insecticides by 50% without sacrificing cotton yield and profitability. In economic terms, the average gross margin for the IPM plot was A$3255 compared with A$3020 and A$3218 for the plots treated with (1) conventional insecticides on transgenic (Ingard®) cotton and (2) conventional insecticides on non-transgenic cotton. The use of transgenic cotton affected a 25% saving on synthetic insecticide usage. The results clearly indicate that a refined IPM approach could have a distinctive advantages in terms of economic and environmental impacts on cotton production. The success of such an approach lies in a strict adherence to its methodology.
Identification of Differentially Expressed Proteins at Four Growing Stages in Chicken Liver

Because of high growth rate and large deposition of fat in the abdomen, the chicken has been used as a model organism for understanding lipid metabolism, fattening and growing. In this study, differentially expression of proteins in chicken liver, one of the important organs for lipid metabolism, has been investigated at four different growing stages. After separation of proteins using two-dimensional electrophoresis (2-DE), more than 700 protein spots were detected. Among them, 13 growing stage specific proteins in chicken liver were selected and further investigated by matrix-assisted laser adsorptions ionization-time of flight mass spectrometry (MALDI-TOF MS). Of these, 12 proteins were matched to existing proteins based on a database search. The identified fat-related proteins in this study were fatty acid synthase (FASN) and malic enzyme (ME1). These proteins were more highly expressed at week 32 than at other weeks. In order to confirm the differential expression, one of the proteins, FASN, was confirmed by western blotting. The identified proteins will give valuable information on biochemical roles in chicken liver, especially for lipid metabolism.
Temperature and diet effects on omnivorous fish performance: Implications for the latitudinal diversity gradient in herbivorous fishes

Herbivorous fishes show a clear latitudinal diversity gradient, making up a larger proportion of the fish spe- cies in a community in tropical waters than in temperate waters. One proposed mechanism that could drive this gradi- ent is a physiological constraint due to temperature. One prediction based on this mechanism is that if herbivorous fishes could shift their diet to animal material, they would be better able to grow, survive, and reproduce in cold wa- ters. We tested this prediction on the omnivore Girella nigricans under different temperature and diet regimes using RNA-DNA ratios as an indicator of performance. Fish had increased performance (100%) at low temperatures (12 °C) when their diet was supplemented with animal material. In contrast, at higher temperatures (17, 22, and 27 °C) fish showed no differences between diets. This indicates that omnivorous fishes could increase their performance at low temperatures by consuming more animal matter. This study supports the hypothesis that a relative increase in the nutri- tional value of plant material at warmer temperatures could drive the latitudinal diversity gradient in herbivorous fishes. Resume : Les poissons herbivores se repartissent selon un gradient latitudinal de diversite, dans lequel ils representent une proportion plus grande des poissons de la communaute dans eaux tropicales que dans les eaux temperees. Un des mecanismes proposes pour expliquer ce gradient est une contrainte physiologique reliee a la temperature. Une des pre- dictions decoulant de ce mecanisme est que, si les poissons herbivores pouvaient changer leur regime alimentaire pour augmenter le materiel animal, ils seraient mieux capables de croitre, de survivre et de se reproduire dans les eaux froi- des. En utilisant les rapports ARN-ADN comme indicateurs de performance, nous avons teste cette prediction chez l'omnivore Girella nigricans a differentes temperatures et sous divers regimes alimentaires. Les poissons ont une per- formance accrue (100 %) a basse temperature (12 C) lorsque leur regime est additionne de matiere animale. En re- vanche, aux temperatures plus elevees (17, 22 et 27 C), il n'y a pas de difference chez les poissons, quel que soit le regime alimentaire. Cela indique que les poissons omnivores pourraient ameliorer leur performance a basses temperatu- res en consommant plus de matiere animale. Notre etude appuie l'hypothese qui veut qu'une augmentation relative de la valeur nutritive de la matiere vegetale aux temperatures plus elevees pourrait expliquer le gradient latitudinal de di- versite chez les poissons herbivores. (Traduit par la Redaction) Behrens and Lafferty 873
Combined application of native Trichoderma isolates possessing multiple functions for the control of Fusarium wilt disease in banana cv. Grand Naine

Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is considered as a lethal disease of bananas worldwide. To manage the disease effectively, 20 rhizospheric and 43 endophytic Trichoderma isolates obtained from 12 different Foc resistant banana accessions were evaluated against Foc in vitro and in vivo. In vitro screening among Trichoderma isolates for their multiple functions (mycelial and spore germination inhibition, hydrogen cyanide, chitinolytic enzymes, non-volatile and volatile metabolites production) in suppressing Foc and promoting plant growth (IAA production and phosphate solubilisation) indicated that the multiple biocontrol actions were significantly higher in 6 isolates of rhizospheric Trichoderma and 10 isolates of endophytic Trichoderma compared to other isolates. The greenhouse evaluation of individual application of these rhizospheric and endophytic Trichoderma isolates against Fusarium wilt pathogen in cv. Grand Naine (AAA) indicated significant suppression of Fusarium wilt disease and increased plant growth characters as compared to Foc pathogen inoculated plants. However, none of these individual Trichoderma isolates recorded complete suppression of Fusarium wilt disease. Therefore, the greenhouse evaluation involving combination of rhizospheric Trichoderma sp. NRCB3 + endophytic Trichoderma asperellum Prr2 recorded 100% reduction of Fusarium wilt disease and increased plant growth parameters up to 250% when compared to individual isolates application and Foc alone-inoculated plants. Further, the field evaluation of this combination of Trichoderma isolates applied for three times: (1) at 15 days before planting, (2) second month after planting and (3) fourth month after planting resulting in significant reduction of Fusarium wilt disease and also increase in bunch weight as compared to untreated control plants. Therefore, these Trichoderma isolates may be used in combination for the effective suppression of Fusarium wilt disease in banana.
A Randomized, Controlled Phase 2 Study of AMG 317, an IL-4Ralpha Antagonist, in Patients With Asthma

Rationale/objective: The impact of air pollution exposure on the development of asthma allergies and related symptoms is not known. This is due to a limited prospective cohort investigation with insufficient longitudinal follow-ups to ascertain this condition. The authors’ objective was to evaluate the relationship between trafficassociated air pollution and development of asthma, allergy and related symptoms in a prophylactic birth cohort setting in an 8-year longitudinal setting.
Ectropion

Ectropion is the most frequent eyelid malposition, characterized by the eversion of the margin and exposure of the conjunctiva and the cornea. It is classified as congenital (primary and secondary) and acquired (involutional, paralytic, mechanical and cicatricial). Congenital ectropion is rare and it is usually associated with other malformations such as euryblepharon, ptosis, epicanthus inversus, and blepharophimosis syndrome. Involutional ectropion is the most frequent form of acquired eyelid eversion and a result of multiple factors. The mechanisms involved in its pathogenesis are discussed in this review. Cicatricial ectropion is caused by the shortening of the anterior lamella of the lid, secondary to congenital malformations, trauma, burns, skin conditions, scarring skin tumors, medications, allergies, blepharoplasty complications, and involutional changes that result in loss of skin elasticity. Mechanical ectropion is caused by eyelid tumors or inflammatory disorders that cause orbicularis spasm. The evaluation of the patient, selection of treatment and surgical techniques are described in detail.
Source of Paralytic Shellfish Toxin in the Bay of Fundy

Seasonal rise in shellfish toxicity in the Bay of Fundy in 1961 was coincident with increased abundance of a marine dinoflagellate, Gonyaulax tamarensis, and the appearance of toxin in plankton extracts. Experiments with unialgal cultures of G. tamarensis isolated from the Bay of Fundy provided overwhelming evidence that it is the primary source of toxin in shellfish in this area and the Gulf of St. Lawrence. G. tamarensis is widely distributed in the North Atlantic and there is evidence of toxic and non-toxic strains of this species.
Field assessment of cadmium, lead and zinc contamination of soils and leaf vegetables under urban and peri-urban agriculture in northern Nigeria

Urban and peri-urban agriculture in dry semi-arid northern Nigeria relies on untreated wastewater for all-year irrigation and the production of vegetables for urban markets. Human and animal exposure to potentially toxic metals is attributed to the consumption of vegetables raised in metal-polluted soils. The objective of this study was to determine the bioavailability and soil–plant transfer of Cd, Pb and Zn to amaranthus (Amaranthus caudatus) and lettuce (Latuca sativa) raised in the garden fields and to assess their safety for human consumption. Ten farmers’ fields were selected per location for analysis of Cd, Pb and Zn in soils and vegetables. Whereas total concentrations of Cd and Zn were greater than the safe or permissible limits for agricultural soils, the Pb concentration was less than its maximum allowable concentration. However, the concentration of Pb and Cd in edible portions of amaranthus exceeded the safe limit for human consumption by 7–13 times, while lettuce exceeded the limit by 11–17 times. Cadmium was more rapidly transferred from soil through root to shoot than Zn > Pb. The plant tissue concentrations of the metals were not significantly correlated with the Diethylene triamine pentaacetic acid (DTPA) and dilute CaCl2-extractable concentrations of the metals in the soils. Furthermore, permissible limit of Pb established as standards for agricultural lands may not be suitable to ensure produce safety in Urban and peri-urban agriculture (UPA) in the city of Kano.
Efeito de diferentes reguladores de crescimento na regeneração in vitro de pau-rosa (Aniba rosaeodora Ducke)

The objective of this work was to establish a protocol for in vitro regeneration of rosewood (Aniba rosaeodora Ducke), using apical and nodal segments inoculated in culture medium with various concentrations of growth regulators. Explants disinfected with solutions of benomyl (4,0 g.L-1) for 24 hours and sodium hipoclorite in 20% + tween 20 for 20 minutes were submitted in an experiment of shoot, root and callus induction in MS medium, with 30 g.L-1 of sucrose and 9,0 g.L-1 of agar added with the growth regulators: BAP (0,0 and 4,0 mg.L-1), ANA, AIA and 2,4-D (0,0; 3,0 and 6,0 mg.L-1), and their respective combinations. The design was complete randomized in arranged factorial 7 X 2, with 14 treatments and 15 replications, where about the explants were analysed the number of shoot, root and callus . After 90 days, the results showed that the presence of auxins is fundamental to induce buds, roots and callus . The medium was contained 4,0 mg.L-1 BAP + 6,0 mg.L-1 AIA showed the best average for shooting with 2,13 buds/explants. In the rooting, the medium was contained 3,0 mg.L-1 of ANA was the more efficient, showed 2,53 roots/explant. However, all the treatments obtained formation of callus, but the medium with 4,0 mg.L-1 BAP + 6,0 mg.L-1 2,4-D showed the best result,1.67 callus/explant.
Distinct hyperintense MRI signal changes in the corticospinal tracts of a patient with motor neuron disease

Neuroimaging in motor neuron disease extensive (MND)is currently performed to exclude other pathologies, though abnormalities may be seen which are consistent with the diagnosis. We report a patient with rapidly progressive MND exhibiting changes on a variety of contemporary magnetic resonance images, which is most likely to represent severe corticospinal tract degeneration. (Amyot Lat Scler 1999; 1:41–44)
Comparison of Three Teaching Methods on 4- Through 7-Year-Old Children's Understanding of the Lungs in Relation to a Peak Flow Meter in the Management of Asthma: A Pilot Study

This pilot study of 19 children compared three methods of teaching 4- through 7-year-old children about their lungs in relation to a peak flow meter. One group saw a doll with three-dimensional lungs, another group saw a puppet with inflatable lungs, and a comparison group received usual Asthma Clinic education. Knowledge of the lungs was measured pre- and post-teaching through two body knowledge assessment instruments. Four children in the Puppet Group improved their posttest scores, compared to three in the Doll Group and two in the Usual Care Group. Continued psychometric studies of the instruments and similar studies with larger sample sizes need to be conducted.