Miracle-dz/UniFAR-FTUs huggingface dataset:
Amifostine-doxorubicin association causes long-term prepubertal spermatogonia DNA damage and early developmental arrest.

BACKGROUND In a previous study, we found that amifostine provides some protection to the seminiferous epithelium of prepubertal doxorubicin-treated male rats but does not improve their fertility status as adults. Based on these results, a long-term study was undertaken to evaluate the DNA damage caused to spermatogonia and the consequences for embryo development. METHODS Twenty-four male prepubertal rats (30-day-old) were divided into four equal groups and treated with: doxorubicin (D--5 mg/kg), amifostine (A--400 mg/kg), amifostine/doxorubicin (AD--amifostine 15 min before doxorubicin) and control (C--0.9% saline solution). Sixty-four days after the treatment, animals were euthanized and the testes and epididymides were excised. The testes were fixed in Bouin's solution and historesin-embedded for histopathological analysis. Spermatozoa from the cauda epididymides were collected for chromatin structure analyses (Comet Assay and SCSA™). Adult rats (100-day-old) were mated with fertile females for embryo analyses on 2.5, 4.5 and 20 days post-coitum (d.p.c.). RESULTS The seminiferous epithelium histopathology of AD group was better preserved compared with the D group. On the other hand, rats from the D and AD groups presented an increased percentage of sperm DNA strand breaks, as assessed by the comet assay, as well as an increased level of sperm chromatin denaturation, as assessed by the SCSA™ assay. In amifostine-treated groups (A and AD) there was a significant increase in the number of arrested embryos, as observed by the number of oocytes/zygotes on 2.5 d.p.c. , when compared with control and doxorubicin groups; however, this number was increased when the AD group was compared with the A group. CONCLUSIONS These results raise a concern about the effects of the association of these two drugs on the germ cell genome. Amifostine-doxorubicin-exposed rat spermatogonia produced long-term damage on sperm DNA, compromised conceptus development and reduced pregnancy outcome.
STRATEGI BERSAING DALAM RANGKA MENGHADAPI MEA (Studi Kasus Rs. Bhakti Asih Karang Tengah -Tangerang)

ABSTRAK Tujuan penelitian ini adalah untuk mengetahui strategi bersaing dan daya saing Rumah Sakit Bhakti Asih untuk memenangkan persaingan saat ini dan mendatang. Penelitian studi kasus dengan metode kualitatif. Data dikumpulkan melalui observasi, dokumentasi dan wawancara kepada pihak Rumah Sakit maupun kepada pasien yang membayar tunai maupun menggunakan fasilitas BPJS. SWOT digunakan dalam analisis penelitian ini. Hasil penelitian menunjukkan bahwa pentingnya peningkatan skill, smart SDM seluruh jajaran Rumah Sakit, pemberian kompensasi, sarana dan prasarana yang layak mengakibatkan meningkatnya loyalitas karyawandan loyalitas pengguna layanan (pasien) untuk berobat di rumah sakit Bhakti Asih. Berdasarkan matrik Space, Rumah Sakit Bhakti Asih ada pada posisi kuadran I dengan kriteria agresif dan strategi yang dijalankan ditekankan pada penggunaan strategi kekuatan-peluang (strength-opportunities). Penggunaan strategi Strenght –opportunites secara bersamaan akan meminimalisir kelemahan dan ancaman yang dihadapi RS. Bhakti Asih. Sehingga RS. Bhakti asih tetap akan menunjukkan keunggulan kompetitifnya. Kata Kunci : Analisis SWOT, Harga, Pelayanan, Prasarana, Strategi Bersaing
Identification and purification of transferrin- and lactoferrin-binding proteins of Bordetella pertussis and Bordetella bronchiseptica

Bordetella pertussis and Bordetella bronchiseptica were both able to grow in iron-deficient medium when supplemented with iron-saturated human lactoferrin or transferrin but not with human apotransferrin. Direct contact between the transferrins and the Bordetella cells did not appear to be required for growth but considerably improved the growth of the organisms. Analysis of B. pertussis and B. bronchiseptica whole-cell lysates from cultures carried out in iron-deficient or iron-replete media revealed iron-repressible proteins (IRPs) of 27 kDa in B. pertussis and of 30, 32, 73.5, and 79.5 kDa in B. bronchiseptica. Iron-inducible proteins of 16, 23.5, 36.5, and 92.5 kDa and of 17, 23.5, 70, 84, and 91 kDa were also identified in B. pertussis and B. bronchiseptica, respectively. By use of affinity chromatography with iron-saturated human lactoferrin or transferrin as ligands, the 27- and 32-kDa IRPs from B. pertussis and B. bronchiseptica, respectively, were specifically isolated. By using iron-chelated affinity columns, we showed that these proteins exhibit an affinity for iron. Cell fractionation experiments indicated that both of these proteins are probably associated with the outer membrane. Growth of the organisms under modulating conditions showed that the production of these IRPs is not under the genetic transcriptional control of vir or bvg, the general virulence regulon in Bordetella spp.
PENGARUH ROE, NPM, TERHADAP HARGA SAHAM DENGAN PENGUNGKAPAN CSR SEBAGAI VARIABEL MODERASI

ABSTRACT This study aims to examine the effect of financial performance on corporate value with corporate social responsibility (CSR) and good corporate governance (GCG) as a moderating variable. The sample in this research is a manufacturing company listed on Indonesia Stock Exchange from 2011-2014 period, selected by using purposive sampling technique so that obtained 25 manufacturing company got 100 observations with krieteria as follows: 1) Company sample is listed in Indonesia Stock Exchange year 2011 - 2014 in a manufacturing company that publishes annual reports in a row. 2) The sample company discloses CSR and GCG in annual report during 2011 - 2014. 3) The sample company did not experience any losses during 2011 - 2014. The data analysis technique used is the regression analysis of moderation. The results of the study refer to the financial performance has a positive influence on the value of the company. CSR is able to strengthen the financial relationship of performance with corporate value. While GCG can strengthen the relationship between financial performance and firm size with firm value. Keywords: Corporate Social Responsibility, Good Corporate Governance, corporate value, financial performance
[Correlation between susceptibility weighted imaging manifestation and serum cystatin C for delayed graft function].

OBJECTIVE To explore the correlation between susceptibility weighted imaging (SWI) manifestation and serum cystatin C level for delayed graft function (DGF). METHODS The conventional MRI, SWI and serum cystatin C of 27 cases with DGF in nephrotransplantation center in Third Affiliated Hospital of Suzhou University from September 2014 and August 2015 were retrospectively analyzed.By contrasting conventional MRI images of transplanted kidney in DGF, the imaging manifestations of benign tumors such as cysts and angiomyolipomas were excluded on SWI images, and then making the renal cortex as the reference, if the abnormal signal lesions were found in the transplanted kidney, the location and signal intensity would be analyzed. The differences in serum cystatin C level between DGF groups without and with abnormal signal lesions were compared by using independent-sample t-test.The correlation between SWI manifestation and serum cystatin C level for DGF was assessed with Spearman rank correlation analysis. RESULTS A total of 15 cases were found without abnormal signal lesions and the average value of their serum cystatin C level was (2.92±0.44) mg/L.A total of 12 cases were found with abnormal low signal lesions located at junctional zone between cortex and medulla, and the average value of their serum cystatin C level was (6.91±0.96) mg/L. The differences in serum cystatin C level between the two DGF groups were statistically significant (t=-4.040, P=0.000). There was a positive correlation between the abnormal low signal lesions on SWI and serum cystatin C level (r=0.660, P=0.000). CONCLUSION The status of renal function impairment could be reflected by being with or without abnormal signal lesions on SWI. A relatively big renal function impairment may be predicted by the appearance of abnormal low signal lesions at junctional zone between cortex and medulla on SWI.
Inflammatory stimuli promote growth and invasion of pancreatic cancer cells through NF-κB pathway dependent repression of PP2Ac

ABSTRACT Previous studies have indicated that inflammatory stimulation represses protein phosphatase 2A (PP2A), a well-known tumor suppressor. However, whether PP2A repression participates in pancreatic cancer progression has not been verified. We used lipopolysaccharide (LPS) and macrophage-conditioned medium (MCM) to establish in vitro inflammation models, and investigated whether inflammatory stimuli affect pancreatic cancer cell growth and invasion PP2A catalytic subunit (PP2Ac)-dependently. Via nude mouse models of orthotopic tumor xenografts and dibutyltin dichloride (DBTC)-induced chronic pancreatitis, we evaluated the effect of an inflammatory microenvironment on PP2Ac expression in vivo. We cloned the PP2Acα and PP2Acβ isoform promoters to investigate the PP2Ac transcriptional regulation mechanisms. MCM accelerated pancreatic cancer cell growth; MCM and LPS promoted cell invasion. DBTC promoted xenograft growth and metastasis, induced tumor-associated macrophage infiltration, promoted angiogenesis, activated the nuclear factor-κB (NF-κB) pathway, and repressed PP2Ac expression. In vitro, LPS and MCM downregulated PP2Ac mRNA and protein. PP2Acα overexpression attenuated JNK, ERK, PKC, and IKK phosphorylation, and impaired LPS/MCM-stimulated cell invasion and MCM-promoted cell growth. LPS and MCM activated the NF-κB pathway in vitro. LPS and MCM induced IKK and IκB phosphorylation, leading to p65/RelA nuclear translocation and transcriptional activation. Overexpression of the dominant negative forms of IKKα attenuated LPS and MCM downregulation of PP2Ac, suggesting inflammatory stimuli repress PP2Ac expression NF-κB pathway–dependently. Luciferase reporter gene assay verified that LPS and MCM downregulated PP2Ac transcription through an NF-κB–dependent pathway. Our study presents a new mechanism in inflammation-driven cancer progression through NF-κB pathway–dependent PP2Ac repression.
Alteraciones de la comunicación social: pragmalingüística

Cuando se oye o se lee una frase, suele ser sencillo determinar si es sintacticamente correcta, si tiene sentido o es semanticamente coherente, y si las palabras se han pronunciado de forma foneticamente aceptable. Ademas, no suele ser dificil determinar si una frase hablada parece aceptable o correcta respecto a su prosodia, como cuando se formula una frase como una pregunta con un patron de entonacion creciente (p. ej., ? eso era una lechuza? ) o cuando las palabras de la frase se pronuncian con demasiada rapidez o con excesiva lentitud, o demasiado alto o demasiado bajo. En muchos casos, la determinacion de la buena conformacion sintactica de una frase, su interpretacion semantica, y la correccion de la pronunciacion y la prosodia se pueden realizar sin utilizar la informacion ajena a la frase (como otras pronunciaciones que rodean a la pronunciacion en concreto, el contexto en que se produjeron, las intenciones de quien habla y de quien escucha). De manera informal, se utiliza el conocimiento natural del idioma para realizar estos juicios de correccion, aunque es dificil especificar con exactitud que aspecto de la frase suena correcto o incorrecto o que norma se ha seguido correctamente o se ha violado. Por lo general, este conocimiento natural tambien se utiliza para valorar las capacidades de lenguaje expresivo y receptivo del nino, pero esta aumentado y se considera en combinacion con pruebas y mediciones normalizadas del lenguaje y del habla. Sin embargo, el tema central de este articulo se ocupa de como utiliza el orador el lenguaje y los gestos para alcanzar sus objetivos interactivos y los de los demas en el contexto de la vida diaria. Este tema se suele describir con distintas expresiones, como la competencia de comunicacion social, la competencia sociolinguistica, la etnografia de la comunicacion o la pragmalinguistica [1-3]. Aqui, la atencion se centra en el desarrollo y las alteraciones de la pragmalinguistica [1,4-7]. Desde esta
Weight changes in critically ill patients evaluated by fluid balances and impedance measurements

Objective.To study simple, rapid, and predictive methods to determine body weight changes in critically ill patients. Design.Prospective, consecutive sample. Setting.Medical intensive care unit of a university hospital. Patients.Thirty-one consecutive patients. Interventions.Calculated weight changes, using day-to-day and cumulative fluid balances corrected (in two ways) for insensible losses, were compared with the actual weight changes (mattress bascule). Atetrapolar impedance technique measuring resistance was evaluated for estimating weight changes. Measurements and Main Results.No reliable relationship was found between calculated weight changes using fluid balances corrected for insensible loss and the observed weight changes. An intraindividual relationship was found between actual weight changes and changes in resistance measured with the tetrapolar impedance technique in a group of 24 critically ill patients with large weight changes (11.1 ± 6.7 kg). No such intraindividual relationship was found in seven patients with small weight changes (3.1 ± 2.2 kg). In each patient, the dope coefficient of the change in weight and resistance relationship differed; this individual slope coefficient could be an indication for hydration. Conclusions.Calculated fluid balances are not predictive for actual weight changes in critically ill patients. Absolute weight measurements are indispensable. Changes in resistance corre with weight changes in individual patients if weight changes were >3 kg. (Crit Care Med 21:871–877)
Multimodal imaging of mouse development: Tools for the postgenomic era

With the sequence of the mouse genome known, it is now possible to create or identify mutations in every gene to determine the molecules necessary for normal development. Consequently, there is a growing need for advanced phenotyping tools to best understand defects produced by altering gene function. Perhaps nothing is more satisfying than to directly observe a process in action; to disturb it and see for ourselves how the process changes before our very eyes. No doubt, this desire is what drove the invention of the very first microscopes and continues to this day to fuel progress in the field of biological imaging. Because mouse embryos are small and develop embedded within many tissue layers within the nurturing environment of the mother, directly observing the dynamic, micro‐ and nanoscopic events of early mammalian development has proven to be one of the greater challenges for imaging scientists. Here, I will review some of the imaging methods being used to study mouse development, highlighting the results obtained from imaging. Developmental Dynamics 235:2386–2400, 2006. © 2006 Wiley‐Liss, Inc.
Study of wheel rim impact test using finite element analysis

This study measures the impact energy absorbed experienced by the wheel rim under dynamic loading. Computational simulation is time saving, and in contrast the wheel impact experiments involve high cost including the manpower. Alloy wheel is widely used due to its excellent performance and appearance. Wheel rim is modelled using CATIA and imported to ABAQUS for further finite element analysis. Yield strength affects the energy absorbing capacity of a material. Aluminium 6061-T1 is found to have the greatest energy absorption value compared to the other materials.
Performance Analysis of Mass Storage Service Alternatives for Distributed Systems

The authors consider the performance of alternative mass-storage services for a client-server-style distributed system. Some qualitative arguments are presented on the ramifications of implementations of mass-storage services at various levels of the storage semantics hierarchy. The authors concentrate, in particular, on contrasting disk and file services. The functionalities of disk and file services are distinguished by their primitive operations: individual disk-block access for the disk service, and individual file-block access for the file service. This difference results in different partitionings of the computation between the client and server, as well as different network communication requirements. To understand the ramifications of such differences between the services, the authors present performance estimates for basic disk and file services. Performance estimates for several design alternatives are presented. >
Pannier is a transcriptional target and partner of Tinman during Drosophila cardiogenesis.

During Drosophila embryogenesis, the homeobox gene tinman is expressed in the dorsal mesoderm where it functions in the specification of precursor cells of the heart, visceral, and dorsal body wall muscles. The GATA factor gene pannier is similarly expressed in the dorsal-most part of the mesoderm where it is required for the formation of the cardial cell lineage. Despite these overlapping expression and functional properties, potential genetic and molecular interactions between the two genes remain largely unexplored. Here, we show that pannier is a direct transcriptional target of Tinman in the heart-forming region. The resulting coexpression of the two factors allows them to function combinatorially in the regulation of cardiac gene expression, and a physical interaction of the proteins has been demonstrated in cultured cells. We also define functional domains of Tinman and Pannier that are required for their synergistic activation of the D-mef2 differentiation gene in vivo. Together, these results provide important insights into the genetic mechanisms controlling heart formation in the Drosophila model system.
Glycodelin-A expression in the uterine cervix.

OBJECTIVE Local immunosuppressive factors in the uterine cervix infected by human papillomavirus are felt to facilitate the malignant transformation process. Glycodelin-A is an immunosuppressive peptide found in several tissues of müllerian origin, most notably the pregnant and decidualized endometrium. Its expression in the uterine cervix has not been defined but could theoretically contribute to the immunopermissive environment of the cervix. To determine whether glycodelin-A is found in the cervix we examined the squamous and endocervical epithelia from both normal and neoplastic cervical specimens from 14 women. METHODS Immunohistochemisty identification of glycodelin-A was performed on archival paraffin-embedded sections from 10 hysterectomies and 4 cone biopsies. Sections were evaluated and staining was scored as negative, positive, or strongly positive with a separate score for the squamous and glandular components of the cervix. RESULTS Eleven of 14 cases, 79%, demonstrated positive staining of the squamous epithelium. Glycodelin-positive cases included hisologically normal (n = 4; 3 strongly positive, 1 positive) as well as dysplastic (n = 5; 1 strongly positive, 2 positive, and 2 negative) and malignant squamous cells (n = 5; 1 strongly positive, 3 positive, and 1 negative). Normal glandular epithelia were negative in all cases but 1, which demonstrated significant squamous and tubal metaplasia of the endocervical glands involved. CONCLUSION Glycodelin-A is found in the squamous epithelium of both the histologically normal and the neoplastic cervix. Further characterization of these results will focus on the possible immunosuppressive effect glycodelin-A may have in the cervix.
Oligosaccharide‐mediated interactions of the envelope glycoprotein gp120 of HIV‐1 that are independent of CD4 recognition

In this study carbohydrate-mediated interactions of the envelope glycoprotein, gp120, of HIV-1 were investigated. Oligosaccharide probes (neoglycolipids), prepared from the N-glycosidically-linked chains of the natural and recombinant forms of gp120, were used in conjunction with the intact glycoprotein to investigate reactivities with a soluble carbohydrate-binding protein (lectin) known as mannose-binding protein in human serum. Evidence is presented that the high-mannose-type oligosaccharides with seven, eight and nine mannose residues from both forms of gp120 are recognized by the serum lectin, and that these reactivities are unrelated to CD4 recognition. Reactivities of the two forms of envelope glycoprotein with macrophages derived from human blood monocytes and with the mannose-specific macrophage endocytosis receptor isolated from human placental membranes were also investigated. Evidence is presented that both forms of gp120 bind to the macrophage surface by multiple interactions in addition to CD4 binding, and that among these interactions is a carbohydrate-mediated binding to the endocytosis receptor. We propose that such carbohydrate-mediated interactions could form the basis of viral attachment to a variety of healthy and diseased tissues.
Study of The Determinants Existence of External Assurance on Sustainability Reports in Indonesia

Due to the growing trend of firms publishing stand-alone sustainability reports, adhering to an unprecedented demand for disclosure, sustainability reports are now equally as important as financial reports. Following this occurrence, subjective and partial disclosures with external assurance are required to assure and maintain credibility of information within sustainability reports. Thus, the purpose of this research is to obtain empirical evidence related to the determinants that affect the existence of external assurance on sustainability reports in Indonesia. The sample being tested are 116 companies registered in the Indonesia Stock Exchange (IDX) during the period 2013–2015, which are issuing stand-alone sustainability reports. The logistic regression model is employed within hypothesis testing. The result showed that both Profitability and Industry Affiliation positively and significantly affect the Existence of External Assurance on Sustainability Reports, meanwhile, Firm Size had positive influence but not significant. Sustainability Department was also found to be an insignificant determinant to the Existence of External Assurance on Sustainability Reports.
A Generic Channel Model For MIMO Systems

In this paper, we propose a general scattering geometry-based channel model for the analysis and design of multiple-input multiple-output (MIMO) systems in mobile fading channels, which describes the propagation environment of wireless signals. According to this model, some important effects can be explained, such as polarization properties of the antennae, large delay and angle dispersion by propagation via far clusters, and the high -rank effect. Meanwhile, a formula can be deduced on the basis of this model to determine the channel transfer matrices and simulate the capacity as well.