Miracle-dz/UniFAR-FTUs huggingface dataset:
Perturbation of cell cycle of human leukaemic myeloblasts in vivo by cytosine arabinoside.

The effect of a single intravenous flash injection of cytosine-arabinoside (ARA-C) (2.0–2.5 mg/kg BW) on the cell cycle of leukaemic myeloblasts in vivo was studied in 7 patients with acute myeloid leukaemia. It was found that the 3H-thymidine labelling index, the mitotic index and the mean grain count of the cells showed a transitory decrease but within the observation period (36 h) returned to pretreatment values. Microspectrophotometric measurements of single cell DNA content suggested an undisturbed flux of cells into DNA synthesis from G1 phase. The findings are in accordance with the assumption that ARA-C arrests cells in DNA synthesis without directly interfering with the progression of cells through G1, G2, or mitosis. Problems of cell synchronization and recruitment due to cytotoxic therapy are discussed.
Active Current Source IGBT Gate Drive With Closed-Loop di/dt and dv/dt Control

This paper proposes an active current source gate drive (ACSD) method based on voltage controlled current source(VCCS) feedback control strategy for high-power IGBTs. Unlike the common voltage source gate drive, the proposed ACSD method provides constant drive current to charge and discharge an IGBT. With a large gate drive current, high switching speed and low switching losses can be achieved in a power converter. However, a high-current/voltage overshoot occurs. To solve this problem, a feedback current proportional to the <inline-formula> <tex-math notation="LaTeX">$\rm{di}/ \rm{dt}$</tex-math></inline-formula> or <inline-formula><tex-math notation="LaTeX"> $\rm{dv}/ \rm{dt}$</tex-math></inline-formula> signal is generated to the IGBT gate. Thus, direct control of the net gate drive current is produced. Then, the current/voltage overshoot is controlled with little sacrifice in switching time, and the switching losses are lower than that with the conventional gate drive method operating simply by gate resistance switchover. The operation principle and circuit implementation of the proposed ACSD method are presented. The experimental results from a 1200 V/800 A IGBT module verify the performance of the proposed method.
Criteria for stability of Markovian jump singular systems with time-varying delays

In this paper, we consider a class of continuous-time Markovian jump singular systems with time-varying delays. The stability problem for this class of systems is discussed and delay-dependent sufficient conditions such that the systems are regular, impulse free and asymptotically mean square stable are developed in terms of linear matrix inequality (LMI). A numerical example is included to show the effectiveness of the proposed methods.
Multidetector CT in patients suspected of having lumbar disk herniation: comparison of standard-dose and simulated low-dose techniques.

PURPOSE To compare standard-dose and simulated low-dose multidetector computed tomography (CT) in patients suspected of having lumbar disk herniation. MATERIALS AND METHODS The institutional review board approved the research protocol with a waiver of patient informed consent. Sixty consecutive patients underwent multidetector CT with four detector rows at 1 mm collimation at 140 kVp, with tube current-time product adapted to body mass index (BMI): 200 (BMI< 22 kg/m(2)), 300 (BMI > or =22 to <30 kg/m(2)), and 400 effective mAs (BMI > or =30 kg/m(2)). Simulated doses at 65%, 50%, 35%, and 20% of the dose were used for acquisition. During two separate sessions, three independent radiologists coded each of three caudal disks as normal, bulging, or herniated and graded canal and foramen compromise. Median numbers of discrepancies between the standard and reduced doses were compared with Friedman and Wilcoxon tests. Agreements within and between readers were evaluated through kappa statistics. RESULTS Dose reduction had no effect on a reader's ability to identify bulging disks (P = .128) and left and right foramen compromises (P = .413 and .665, respectively). However, for normal disks (P = .002), herniated disks (P = .004), and canal compromise (P = .002), dose reduction did have a significant effect. For normal disks and canal compromise, a reduction dose effect was not detected at 65% (P = .121 and .250, respectively) but appeared at 50% (P = .004 and .008, respectively). For herniation, a dose reduction effect was detected at 35% (P = .031). Agreements within and between readers ranged from poor to excellent and tended to decrease with dose reduction. CONCLUSION For patients suspected of having lumbar disk herniation, tube charge settings could be reduced to 65% of the standard dose adapted to the BMI.
Neoadjuvant capecitabine and oxaliplatin followed by synchronous chemoradiation and total mesorectal excision in magnetic resonance imaging-defined poor-risk rectal cancer.

PURPOSE To evaluate neoadjuvant capecitabine/oxaliplatin before chemoradiotherapy (CRT) and total mesorectal excision (TME) in newly diagnosed patients with magnetic resonance imaging (MRI) -defined poor-risk rectal cancer. PATIENTS AND METHODS MRI criteria for poor-risk rectal cancer were tumors within 1 mm of mesorectal fascia (ie, circumferential resection margin threatened), T3 tumors at or below levators, tumors extending > or = 5 mm into perirectal fat, T4 tumors, and T1-4N2 tumors. Patients received 12 weeks of neoadjuvant capecitabine/oxaliplatin followed by concomitant capecitabine and radiotherapy. TME was planned 6 weeks after CRT. Postoperatively, patients received another 12 weeks of capecitabine. RESULTS Between November 2001 and August 2004, 77 eligible patients were recruited. After neoadjuvant capecitabine/oxaliplatin, the radiologic response rate was 88%. In addition, 86% of patients had symptomatic responses in a median of 32 days (ie, just over one cycle of capecitabine/oxaliplatin). After CRT, the tumor response rate was increased to 97%. Three patients remained inoperable. Sixty-seven patients proceeded to TME, and all but one patient had R0 resection. Pathologic complete response was observed in 16 patients (24%; 95% CI, 14% to 36%), and in an additional 32 patients (48%), only microscopic tumor foci were found on surgical specimens. Four deaths occurred during neoadjuvant capecitabine/oxaliplatin therapy as a result of pulmonary embolism, ischemic heart disease, sudden death with history of chest pain, and neutropenic colitis. CONCLUSION Capecitabine/oxaliplatin before synchronous CRT and TME results in substantial tumor regression, rapid symptomatic response, and achievement of R0 resection.
TMKink: A method to predict transmembrane helix kinks

A hallmark of membrane protein structure is the large number of distorted transmembrane helices. Because of the prevalence of bends, it is important to not only understand how they are generated but also to learn how to predict their occurrence. Here, we find that there are local sequence preferences in kinked helices, most notably a higher abundance of proline, which can be exploited to identify bends from local sequence information. A neural network predictor identifies over two‐thirds of all bends (sensitivity 0.70) with high reliability (specificity 0.89). It is likely that more structural data will allow for better helix distortion predictors with increased coverage in the future. The kink predictor, TMKink, is available at http://tmkinkpredictor.mbi. ucla.edu/.
Adaptive feedback equalization receiver for DS-CDMA with turbo coded systems

In this paper, adaptive equalizer receiver for a turbo code direct sequence code division multiple access (DS-CDMA) by using least mean square (LMS) adaptive algorithm is presented. The proposed adaptive equalizer is based on finite impulse response (FIR) filter structure in which included conventional adaptive equalizer, decision feedback adaptive equalizer and feedback adaptive equalizer. The objective of the proposed equalizer is to minimize the bit error rate of the data due to the disturbances of noise and intersymbol interference phenomenon on the channel of the digital communication system. The computer simulation results shown that the proposed feedback adaptive equalizer provides a good bit error rate than the others one such as conventional adaptive equalizer, decision feedback adaptive equalizer
Telehealthcare for long term conditions

#### Summary points Telehealthcare is the provision of personalised healthcare over a distance.1 It has the three following essential components2w1: Telehealthcare can be delivered by both synchronous and asynchronous (such as store and forward) technologies (fig 1⇓). For example, telephone and video conferencing enable consultations in real time. An example of asynchronous communication would be storing two weeks’ of spirometry results in a batch and forwarding these on to a healthcare provider, who responds by email or telephone. Telehealthcare is related to, but distinct from telemedicine, where technology is used to share information over a distance between healthcare providers.2 Fig 1 Key elements of telehealthcare #### Sources and selection criteria We identified systematic reviews and original research studies on telehealthcare and long term conditions. We used searches from our ongoing Cochrane systematic reviews to find randomised controlled trials for telehealthcare interventions for asthma and chronic obstructive pulmonary disease. Search terms were telehealth, tele-health, telemedicine, tele-medicine, internet, computer, web, interactive, telecommunication, telephone, …
Flexible MIM capacitors using zirconium-silicate and hafnium-silicate as gate-dielectric films

To the first time, we have fabricated metal-insulator-metal (MIM) capacitors using 10-nm-thick zirconium-silicate (ZrSi<inf>x</inf>O<inf>y</inf>) and hafnium-silicate (HfSi<inf>m</inf>O<inf>n</inf>) thin dielectric films on the flexible polyimide substrate by sol-gel process. The sol-gel films were oxidized by employing oxygen plasma to enhance the electrical performance at low temperature (∼250 °C). The oxygen plasma may accept as most effective process at low temperature to surface oxidation of a dielectric film for flexible organic device. The results showed the satisfactory electrical characteristics for the corresponding films with low leakage current densities ∼10<sup>−9</sup> Acm<sup>−2</sup> at 5V and maximum-capacitance densities 12.10 (ZrSi<inf>x</inf>O<inf>y</inf>) and 14.32 fF/µm<sup>2</sup> (HfSi<inf>m</inf>O<inf>n</inf>), at 1MHz. These entire make the combinatorial thin oxide films based MIM capacitors to be very suitable for future flexible devices.
In vitro immunotoxicology of quantum dots and comparison with dissolved cadmium and tellurium

The increasing use of products derived from nanotechnology has raised concerns about their potential toxicity, especially at the immunocompetence level in organisms. This study compared the immunotoxicity of cadmium sulfate/cadmium telluride (CdS/Cd‐Te) mixture quantum dots (QDs) and their dissolved components, cadmium chloride (CdCl2)/sodium telluride (NaTeO3) salts, and a CdCl2/NaTeO3 mixture on four animal models commonly used in risk assessment studies: one bivalve (Mytilus edulis), one fish (Oncorhynchus mykiss), and two mammals (mice and humans). Our results of viability and phagocytosis biomarkers revealed that QDs were more toxic than dissolved metals for blue mussels. For other species, dissolved metals (Cd, Te, and Cd‐Te mixture) were more toxic than the nanoparticles (NPs). The most sensitive species toward QDs, according to innate immune cells, was humans (inhibitory concentration [IC50] = 217 μg/mL). However, for adaptative immunity, lymphoblastic transformation in mice was decreased for small QD concentrations (EC50 = 4 μg/mL), and was more sensitive than other model species tested. Discriminant function analysis revealed that blue mussel hemocytes were able to discriminate the toxicity of QDs, Cd, Te, and Cd‐Te mixture (Partial Wilk's λ = 0.021 and p < 0.0001). For rainbow trout and human cells, the immunotoxic effects of QDs were similar to those obtained with the dissolved fraction of Cd and Te mixture. For mice, the toxicity of QDs markedly differed from those observed with Cd, Te, and dissolved Cd‐Te mixture. The results also suggest that aquatic species responded more differently than vertebrates to these compounds. The results lead to the recommendation that mussels and mice were most able to discriminate the effects of Cd‐based NPs from the effects of dissolved Cd and Te at the immunocompetence level. © 2013 Wiley Periodicals, Inc. Environ Toxicol 30: 9–25, 2015.
Dissolution, Solubility, XRD, and DSC Studies on Flurbiprofen-Nicotinamide Solid Dispersions

Flurbiprofen-nicotinamide solid dispersions were prepared by the fusion method. The solid dispersions were evaluated for dissolution rate. The drug-carrier interaction in the liquid and solid states were studied by using phase solubility analysis, phase diagram, X-ray diffraction (XRD), and differential scanning calorimentry (DSC). Solid dispersions gave fast and rapid dissolution of flurbiprofen compared with the pure drug and the physical mixture. Phase diagram and DSC indicated that flurbiprofen and nicotinamide form a eutectic mixture. The aqueous solubility of flurbiprofen was enhanced in the presence of nicotinamide.
Locus of fragility in robust breast cancer system

Functional heterogeneous redundancy of breast cancer makes this tumor to be robust. Signaling mechanisms which control cancer responses are crucial for controlling robustness. Identification of locus of fragility in cancer represents basic mechanism to target robustness. The goal of this prospect is to present locus of fragility in breast cancer robust system, and how disruption of this locus induces failure of robustness. My recent research show, that locus of fragility in breast cancer cells is suppression of nitric oxide (NO). When it was targeted, dynamics of cancer to generate robustness failed that it blocked cancer cell proliferation dependent on the NO/Rb pathway, blocked cell migration and angiogenesis dependent on the VEGF/PI3K/AKT/NO/ICAM‐1 pathway, and induced breast cancer cell apoptosis through the NO/ROCK/FOXO3a signaling pathway. This tiny and trivial perturbation in breast cancer cells such as suppression of NO represents locus of fragility (weakness) and new approach for breast cancer chemotherapy. © 2004 Wiley‐Liss, Inc.
Dielectric characterization of collagen, elastin, and aortic valves in the low temperature range

The low temperature dielectric relaxation of porcine aortic valves and its main macromolecular proteins, i.e. elastin and collagen, have been investigated in the dry state and at low levels of hydration by thermally stimulated currents spectrometry, with an equivalent frequency of 10-3 Hz. Two secondary relaxation modes, labeled γ and β with increasing temperature, are found for the three materials. Since the γ-mode is independent upon hydration while the β-mode is strongly plasticized by water, these relaxation modes have been attributed to localized motions of the polypeptidic chains containing apolar and polar residues, respectively. The deconvolution of the β-mode by fractional polarization gives the experimental distribution of the dielectric relaxation times of the three materials, and allows us to deduce the activation parameters of each elementary process. These analyses shows the existence of compensation phenomena between the activation parameters, implying cooperative mechanisms. The occurrence of these phenomena with their characteristic parameters are used to specify the origin of the localized relaxation modes in collagen and elastin, and to assign the specific role of each protein in the aortic valves.
A user-centered drowsy-driver detection and warning system

This work is a culmination of years of research to develop an effective in-vehicle countermeasure to drowsy driving. Previous work resulted in an independently validated measure of drowsiness that was then incorporated into a drowsy-driver prototype monitor. The goal of this project was to develop an associated drowsy-driver interface that enabled effective, user-centered interactions with the underlying system.A multidisciplinary team designed a new drowsy-driver interface and introduced smart user interactions through a careful participatory design process that included both design experts and commercial motor vehicle drivers. It is hoped that this effort and subsequent field trials will result in a reliable, smart system that convinces drivers that they are driving in an unsafe condition and to make a wise choiceóstop and rest.
Equivalent circuit model of on-wafer interconnects for CMOS RFICs

An equivalent circuit model of on-wafer interconnects was extracted directly from the S-parameter measurements. In the proposed model, the skin effect and the substrate losses have been considered. Furthermore, an additional element was introduced to predict the fringing effect for the first time. A hybrid genetic algorithm was used for the parameter extraction. The constructed two-/spl Pi/ model is computationally efficient and is shown to be sufficiently accurate for RF applications. The accuracy was demonstrated by the on-wafer measurements of interconnects with various physical dimensions, fabricated on the top-metal layer, employing a 0.18 /spl mu/m RF-CMOS process.
Phosphorylation of the Yeast Heat Shock Transcription Factor Is Implicated in Gene-Specific Activation Dependent on the Architecture of the Heat Shock Element

ABSTRACT Heat shock transcription factor (HSF) binds to the heat shock element (HSE) and regulates transcription, where the divergence of HSE architecture provides gene- and stress-specific responses. The phosphorylation state of HSF, regulated by stress, is involved in the activation and inactivation of the transcription activation function. A domain designated as CTM (C-terminal modulator) of the Saccharomyces cerevisiae HSF is required for the activation of genes containing atypical HSE but not typical HSE. Here, we demonstrate that CTM function is conserved among yeast HSFs and is necessary not only for HSE-specific activation but also for the hyperphosphorylation of HSF upon heat shock. Moreover, both transcription and phosphorylation defects due to CTM mutations were restored concomitantly by a set of intragenic suppressor mutations. Therefore, the hyperphosphorylation of HSF is correlated with the activation of genes with atypical HSE but is not involved in that of genes with typical HSE. The function of CTM was circumvented in an HSF derivative lacking CE2, a yeast-specific repression domain. Taken together, we suggest that CTM alleviates repression by CE2, which allows HSF to be heat-inducibly phosphorylated and presume that phosphorylation is a prerequisite for the activator function of HSF when it binds to an atypical HSE.
Binding of the herpes simplex virus immediate-early gene product ICP4 to its own transcription start site

A gel electrophoresis DNA-binding assay was used to detect proteins from herpes simplex virus type 1-infected and uninfected cells that specifically bind the upstream region of immediate-early (IE) gene 3. The assay is based on the altered electrophoretic mobility of DNA-protein complexes relative to that of free DNA in native gels. A series of end-labeled overlapping DNA fragments spanning a region from -272 to +27 (relative to the 5' terminus of the IE gene 3 mRNA) were used as probes. Two complexes were identified (referred to as A and B) which were driven by different protein factors. Formation of the A complex required infected-cell proteins extracted at any time from 2 to 16 h postinfection; a 0.5 to 1 M NaCl extract of infected cells, and a DNA probe that contained the sequences from -4 to +27 (relative to the 5' terminus of IE gene 3 mRNA). The protein that drove the formation of the A complex is not related to transcription factors TFIIIA or Sp1 or their cognate binding domains since neither the 5S RNA gene nor the GC box of simian virus 40 could compete for proteins that induced formation of the A complex. Through the use of monoclonal antibodies, the complex was shown to contain the IE gene 3 product, ICP4. A more detailed localization of the DNA-binding site in vitro by using chemical footprinting revealed that binding occurs over the sequence from -10 to +3 relative to the mRNA terminus. The binding of ICP4 to its own transcription start site may explain the repression of IE gene transcription which attends the onset of early (beta) gene expression and suggests an autoregulatory mechanism for gene control in herpes simplex virus type 1. The B complex was readily detected in uninfected cells (of a number of different cell lines), as well as in infected cells, with a probe containing the IE consensus sequence TAATGARATTC (where R is a purine) and two nested copies of the Sp1 binding motif GGGCGG; however, complexes were also detected with probes that lack the IE consensus sequence but contain Sp1 sites. These data suggest that the B complex contains the promoter-specific factor Sp1, and competition experiments with the clustered Sp1 binding domains from simian virus 40 confirmed this idea.
Cytokines Regulate Genes Involved in Atherogenesis a

The term cytokines refers to a number of families of proteins characterized biologically and structurally over the last decade. These multipotent mediators of inflammation and immunity have widespread generalized actions in host defenses and in pathology. Our interest in these mediators stems from a large body of evidence that cytokines can affect key functions of vascular wall cells. Moreover, vascular smooth muscle and endothelial cells can themselves produce certain of these mediators. Growing evidence suggests that cytokines may participate as autocrine or paracrine mediators in atherogenesis and various other pathological processes that affect blood vessels. The various functions of vascular wall cells subject to regulation by cytokines may influence atherogenesis at various stages ranging from lesion initiation, through progression and evolution of the lesion, and culminating in complication and clinical manifestations of atheroma. We will illustrate here with selected examples how cytokines regulate genes related to atherogenesis. While our discussion will not encompass the entire gamut of cytokine-mediated alterations of vascular gene expression, this summaly will serve to highlight the potential roles of cytokines in the pathogenesis of this important disease.
La gestión del patrimonio emocional y del conocimiento en las organizaciones

E motionaL capital management in companies and organizations is considered as «capital» to emotions, to identify and manage the fear and sense of belonging to create emotionally sustainable organizations. This approach has a direct impact on knowledge management, whose purpose is to support business strategy, based on a study of the processes of identifying, measuring and use of knowledge available to a company or organization. This theoretical base whose applicability has been recognized by several universities is the starting point for proposing the model of emotional capital management and knowledge - GPEC, understood as an effective model for knowledge management based on emotional management. In this sense, we prove the hypothesis that companies or organizations better manage their emotional capital, also optimally manage their knowledge, obtaining better economic performance, productivity and competitiveness.