Miracle-dz/UniFAR-FTUs huggingface dataset:
Inhibitory and oxidative effects of gossypol on MCF7 Breast Cancer cells in vitro

Human cancer is the second leading cause of death in the United States and breast cancer is responsible for the second highest number of deaths in women with cancer worldwide. Today, cancer is becoming more and more resistant to current chemotherapeutic agents. In an effort to decrease this resistance, natural products like gossypol are being tested for efficacy as a natural chemotherapeutic agent with anticancer properties. Current literature demonstrates that gossypol is indeed an effective drug against breast cancer when used alone and when combined with other chemotherapeutic agents [1-5]. The majority of current literature focuses on the ability of gossypol to antagonize anti-apoptotic proteins like BCL-XL and induce apoptosis [6, 7]. This study helps understand previous data and goes beyond the current knowledge base and explores not only apoptosis induction, but also on other important effects like: oxidative stress, other possible avenues of cell death, growth and development, and cell cycle progression. Combining physiological, genotypic, flow cytometric and biochemical assays, a more complete understanding of gossypol‟s efficacy and mechanism of action can be ascertained. In past studies, the focus of gossypol‟s efficacy has been too narrow and currently the study of gene regulators like microRNAs (miRNAs) has not been incorporated. This study reveals evidence that miRNA may play an important role and that gossypol‟s efficacy is in fact a multi-component system that is interconnected in its overall mechanism of action. Inhibitory and oxidative effects of gossypol on MCF7 Breast Cancer cells in vitro A Thesis Presented To The Faculty of the Department of Biology East Carolina University In Partial Fulfillment of the Requirements for the Degree Master of Science in Molecular Biology and Biotechnology by William Brandon Winfrey 20 August 2013 Inhibitory and oxidative effects of gossypol on MCF7 Breast Cancer cells in vitro by William Brandon Winfrey
Microorganisms and viruses causing diarrhea in infants and primary school children and their relation with age and sex in Zakho city, Kurdistan Region, Iraq

Background: Diarrheal diseases in children are a major public health concern in developing countries. Diarrheal infection spreads through contaminated food or drinking-water, or from person-to-person as a result of poor hygiene. This study was conducted to investigate the incidence of different microorganisms associated with diarrhea in infants and primary school children and their relation to sex and age in Zakho city, Kurdistan region, Iraq. Methods: During the period from September 2013 to May 2014, 600 diarrheic samples were collected from both sexes and different ages ( 4-8 years (65.38 and 64.07%), while parasites were among >6-10 years (64.67 and 60.11%). High viral prevalence were recorded among ages <2 to 6 years with the peak among <2 years (40.54%). Conclusions: From this study we conclude that about 57% of diarrheal cases were associated with bacteria, parasites, and viruses with E. coli, E. histolytica , G. lamblia and rotavirus as leading microorganisms. The mixed prevalence with two or more microorganisms was documented in 179 (37.36%) out of 479 positive samples and the rate of microbial prevalence was found to be gender and age dependent.
Neurociencia y educación: hacia la construcción de puentes interactivos

Introduccion. El debate sobre las relaciones potenciales entre neurociencia y educacion comenzo hace aproximadamente unas tres decadas. La articulacion de conocimientos neurocientificos y educativos implica contemplar que la emergencia de procesos cognitivos y emocionales durante el desarrollo, asi como la posibilidad de influenciarlos a traves de intervenciones especificas, podrian integrarse a los procesos de aprendizaje y ensenanza. Objetivo. Examinar las implicaciones emergentes de los vinculos entre neurociencia, psicologia cognitiva y educacion, y los requisitos para la construccion de puentes interactivos entre diferentes campos de conocimiento. Desarrollo. Dicha construccion implica la reduccion de brechas epistemologicas, metodologicas, tecnologicas y practicas. La consideracion del desarrollo humano y los procesos de aprendizaje en terminos de multiplicidad de niveles de analisis podria contribuir al enriquecimiento de un dialogo critico y a producciones conjuntas genuinas. Se plantean consideraciones epistemologicas y metodologicas, y se presentan diversas propuestas acerca de como establecer los puentes entre las disciplinas. Conclusiones. Dado el estado incipiente de los esfuerzos interdisciplinarios, seria conveniente abordar la construccion de puentes desde diversos angulos, en lugar de establecer un puente unico. Se proponen elementos constitutivos basicos para la generacion de puentes dinamicos orientados a integrar las relaciones complejas involucradas en los procesos de ensenanza y aprendizaje.
Sensibilidad y especificidad de PCR anidada y Spoligotyping como pruebas rápidas de diagnóstico de tuberculosis bovina en tejido fresco Sensitivity and specificity of nested PCR and spoligotyping as quick diagnostic tests for bovine tuberculosis in fresh tissue

Quick diagnosis of tuberculosis in cattle is critical to decide whether or not to quarantine or depopulate a herd. Currently, decisions are based on culture, which takes between four and eight weeks to accomplish. Therefore, the purpose of this study was to evaluate relative sensitivity and specificity of two PCRs (MPB70 and IS6110) and spoligotyping as quick diagnostic tests for cattle tuberculosis in fresh tissue. Fifty tissue samples with TB-suspicious lesions from a herd with 25 % prevalence of the disease and fifty tissue samples with no lesions from a TB-free herd were used in the study. Samples were split into two sets and each set was blind analyzed in two different laboratories under the same protocol. The three tests were used to diagnose tuberculosis in all samples using macerated tissue just before culturing. Relative sensitivity and specificity of all tests were estimated using presence/absence of lesion, histopathology and culture results as gold standards. MPB70 nested PCR showed consistently higher sensitivity (85 to 91 %) and specificity (77 to 86 %) in one of the laboratories with all gold standards; however, inter-laboratory differences occurred, in one of the laboratories sensitivity was from 89 to 91 % and specificity from 57 to 63 %. IS6110 nested PCR and spoligotyping behaved poorly. Relative sensitivity and specificity for MPB70 nested PCR suggest that this test could be useful as a complementary test for quick diagnosis of bovine TB in fresh tissue.
Hiperplasia suprarenal congénita de comienzo tardío

* Medico General Cod. 11812.Universidad Autonoma de Centro America Correo: dr.callau@gmail.com The late onset congenital adrenal hyperplasia, also called Nonclassic, has a much milder clinical presentation than the classic “Salt wasting form”, and is a condition that may go unnoticed or mistaken for polycystic ovary syndrome. The history of newborn screening is not reliable to rule out its presence and if suspected, measurements must be performed (17-hydroxyprogesterone). The treatment of this disease has proven to be useful because it improves the fertility of the affected people but are not yet duly filed regimes. INTRODuCCION
Physical Activity Levels in Children in Age Groups of 10 to 15 Years.

Objective: This study assesses the level of physical activity in children in the age groups of 10 to 15 years. Method: This was cross sectional study design. 358 children between 10 to 15 years of age were selected by convenient sampling from various schools in and around Navi Mumbai. The physical activity level was assessed using a validated questionnaire and compared across the age groups. Results and Conclusion: The boys were observed to have moderate physical activity levels (2.909 +/0.411) while the girls exhibited lower scores of physical activity (2.302 +/0.171). No significant differences in the activity levels were observed across the age groups in both the sexes. No significant association was observed between physical activity and body mass index, although socioeconomic status had some effect on the scores.
Central Venous Catheter in Obese Patient May Even Cause Cor Pulmonale

Introduction: Sometimes the needless usage of central venous catheter, conflicting with indications, has been witnessed. Unfortunately, incidence of axillary-subclavian venous thrombosis has risen gradually because of increased use of the subclavian vein for central venous access. Furthermore obesity is common among patients with deep vein thrombosis, with a prevalence of 20-25%. Presentation of Case: I am presenting a 35-year old morbidly-obese woman with cor pulmonale resulting from post-catheterization thrombosis of jugular, axillary, subclavian veins, and superior vena cava. Discussion: Obese individuals have higher levels of factor VIII and factor IX. Obesity promotes deep vein thrombosis by inducing plasma viscosity and erythrocyte aggregation as well. Obesity causes, via leptin, increasing activity of coagulation cascade and decreasing fibrinolysis. Also it increases inflammation, oxidative stress and endothelial dysfunction. Obesity together with central venous catheter use may increase the risk for the development of deep vein thrombosis and subsequent cor pulmonale. Conclusion: Requirement of central venous catheter must be reconsidered before introducing, especially in an obese patient.
Survey of Ontario health professionals’ knowledge, attitudes and beliefs of alcohol-related harms: key findings

Abstract Introduction: The purpose of this study is to explore the knowledge, attitudes and beliefs among health and health care professionals in Ontario of the risks associated with excessive alcohol use to strengthen attention on alcohol as a key modifiable risk factor for cancer and other health harms. Methods: This study used an online survey to answer the following research questions: (1) What is the level of awareness of excessive alcohol use as a risk factor for injuries, cancer and chronic diseases among health and health care professionals? (2) How do you address alcohol consumption and alcohol-related harm reduction in practice? and (3) What opportunities and barriers exist for health care professionals in addressing alcohol-related harm in practice? Results: Five hundred and eight-one participants responded to the online survey. The majority of respondents were Dietitians, Public Health Nurses, Nurses, Health Promotion Consultants and Health Educators. Results include participant’s attitudes, opinions and knowledge on the identified research questions. Discussion & Conclusion: Results from this survey will be disseminated to promote health and health care professionals awareness surrounding issues of alcohol consumption and alcohol-related harm, with emphasis on physicians and other Allied Health Professionals (including Registered Dietitians and Public Health Nurses). Several knowledge exchange areas are identified.
Arterial tone in chronic renal insufficiency

12 INTRODUCTION 14 REVIEW OF THE LITERATURE 16 1 Chronic renal insufficiency 16 1.1 Pathophysiology and progression of impaired renal function 16 1.2 Vasoactive peptides volume overload 19 1.3 Calcium metabolism and secondary hyperparathyroidism 20 1.4 Cardiovascular complications 23 1.4. 1 Secondary hypertension 23 1.4. 2 Vascular calcification 25 1.4. 3 Atherosclerosis 27 1.5 Renin-angiotensin system and chronic renal insufficiency 28 1.5. 1 Tissue versus circulating renin-angiotensin system 28 1.5. 2 Inhibition of the actions of the renin-angiotensin system 31 2 Local control of arterial tone 34 2.1 Endothelium-derived vasodilatory factors 34 2.1.1 Nitric oxide 34 2.1.2 Prostacyclin 35 2.1.3 Endothelium-derived hyperpolarization 35 2.2 Endothelium-derived contractile factors 37 2.3 Vascular smooth muscle 40 2.3.1 Contraction and cellular calcium regulation 40 2.3.2 Na-K ATPase 41 2.3. 3 K channels 42 3 Arterial tone and structure in chronic renal insufficiency 43 3.1 Conductance arteries 44 3.2 Resistance arteries 45 AIMS OF THE PRESENT STUDY 48
The ABCs of HIV / AIDS prevention -- seeking the evidence and telling the truth.

Over the past 15 years faith-based organizations (FBOs) building on their calling for compassion to the suffering have addressed the issues of stigma and have been leaders in providing care to AIDS victims and their families. They have also developed effective education programs directed towards encouraging sexual abstinence among unmarried youth and fidelity among couples. Correspondingly while holding to their principles regarding abstinence and fidelity many FBOs have come to recognize the ethical obligation of informing people about condoms for the prevention of HIV transmission in high-risk situations for example where multiple partners or commercial sex workers are involved. And at the same time public health professionals have begun to promote reduction in number of partners especially among men having sex with men and situations involving multiple partners. (excerpt)
人参皂苷Rh2调控PI3K/AKT/GSK-3β信号通路诱导人结肠癌细胞凋亡

目的探究人参皂苷Rh2(ginsenoside Rh2, Rh2)诱导人结肠癌细胞SW480凋亡作用机制。 方法CCK-8法检测Rh2对SW480细胞增殖的影响；流式细胞术(Flow cytoMetry, FCM)检测Rh2对SW480细胞凋亡的影响；Hoechst33258染色观察Rh2对SW480细胞凋亡形态学的影响；Western blot检测经Rh2诱导SW480细胞中凋亡相关蛋白Bcl-2、Bax、p53、cleaved caspase-3，PI3K/AKT/GSK-3β信号通路相关蛋白PI3K、AKT、P-AKT、GSK-3β、P-GSK-3β表达量变化；LY294002、Rh2单独及联合诱导SW480细胞后，蛋白PI3K、AKT、P-AKT、GSK-3β、P-GSK-3β表达量变化。 结果CCK-8结果显示Rh2呈时间浓度依赖抑制SW480细胞增殖。 FCM结果显示细胞早期凋亡率由正常对照组的(0.70±0.09)%增至(11.06±1.04)% (P＜0.05)。 Hoechst33258结果显示，Rh2诱导SW480细胞48h后呈现典型的凋亡形态学改变。 Western blot结果显示，经Rh2诱导的SW480细胞，凋亡相关蛋白 Bcl-2表达降低，Bax、p53、激活型胱天蛋白酶3(cleaved caspase-3)蛋白表达增加；PI3K/AKT/GSK-3β信号通路蛋白PI3K、P-AKT、P-GSK-3β表达量与对照组比较明显减少，AKT、GSK-3β表达量无明显变化；LY294002、Rh2和LY294002与Rh2联合诱导SW480细胞后，总AKT蛋白和总GSK-3β蛋白表达量基本一致，LY294002与Rh2联合用药对SW480细胞中PI3K、P-AKT和P-GSK-3β的表达抑制作用较单独用药更加明显。 结论Rh2可能是通过抑制PI3K/AKT/GSK-3β通路，激活p53信号通路，激活caspase-3，破坏Bcl-2/Bax比例，诱导结肠癌细胞SW480凋亡。
Comprehensive geriatric assessment test in elderly cancer patients

Background: Cancer is the first cause of the death among the people with 65–75 years age, and about 60% of cancers are diagnosed at age over 70. In this study, the impact of comprehensive geriatric assessments (CGAs) on the quality and the length of life in disabled elderly cancer patients were evaluated. Patients and Methods: In an interventional study, 70 patients with cancer were randomly divided into two treatment and control groups. The patients were evaluated during a 6-month study using CGA test. Results: The patient's mean age was 4.2 ± 67.1 years. It was shown that CGA intervention had no impact on physical activity tests, including instrumental activities of daily living and activities of daily living, but a significant improvement was shown in patient's psychological status using geriatric depression test. Conclusions: The results of the current study showed that CGA interventions have a positive effect on the quality of the life of the elderly cancer patients.
Sexual Behaviour among Long Distance Truck Drivers

Long distance truck drivers plying on national highways passing through Pune-Ahmednagar State Highway No. 27 who halted at road side group of "Dhabas" (road side hotels) at Shirur in Pune district Maharashtra (India) were enrolled in a cross-sectional study conducted for 5 days in March 2004 between 1800 hrs -- 2300 hrs. A total of 338 truck drivers could be approached over a period of 5 days. Out of these 315 consented for the study. The adequacy of sample size was established using "Stat Cal" menu of WHO-CDC Statistical software Epi Info. The study was conducted at Rural Health Training Centre Shirur District Pune Maharashtra. All interviewers were properly trained and briefed for administering the questionnaire. A group of dhabas was identified on the Pune -- Ahmednagar State Highway number 27. All the truck drivers who stopped at the dhabas were invited to participate and those who consented for the interview were taken to a separate enclosure in order to maintain privacy and confidentiality. On the first day a pilot study of 32 truck drivers was carried out with the help of the questionnaire adopted from UNAIDS. It included questions on knowledge and behaviour on HIV STI and condom usage. The questionnaire was administered using personal face-to-face interview techniques. The questionnaire was suitably modified after the pilot study. The data of this pilot study has not been included in the final analysis. (excerpt)
Aerosol measurements with a shipborne Sun–sky–lunar photometer and collocated multiwavelength Raman polarization lidar over the Atlantic Ocean

Abstract. A shipborne Sun–sky–lunar photometer of type CE318-T was tested during two trans-Atlantic cruises aboard the German research vessel Polarstern from 54∘ N to 54∘ S in May/June and December 2018. The continuous observations of the motion-stabilized shipborne CE318-T enabled the first-time observation of a full diurnal cycle of aerosol optical depth (AOD) and column-mean Ångström coefficient of a mixed dust–smoke episode. The latitudinal distribution of the AOD from the shipborne CE318-T, Raman lidar and MICROTOPS II shows the same trend with highest values in the dust belt from 0 to 20∘ N and overall low values in the Southern Hemisphere. The linear-regression coefficients of determination between MICROTOPS II and the CE318-T were 0.988, 0.987, 0.994 and 0.994 for AODs at 380, 440, 500 and 870 nm and 0.896 for the Ångström exponent at 440–870 nm. The root-mean-squared differences of AOD at 380, 440, 500 and 870 nm were 0.015, 0.013, 0.010 and 0.009, respectively.
Type 2 diabetes in people of South Asian origin: potential strategies for prevention

South Asians have a one in three lifetime risk for the development of diabetes, developing the condition ten years earlier than Europids. The high social and economic burden of diabetes in South Asians is augmented by greater rates of attrition from renal and cardiovascular disease. This article reviews the epidemiology and pathogenesis of diabetes in South Asians, and potential strategies by which the condition could be prevented or delayed in this high risk group. Br J Diabetes Vasc Dis 2007;7:279-82
Cancers secondaires après allogreffe de cellules souches hématopoïétiques

Cet article de revue cherche a repondre a quatre questions: 1) quelle est l'incidence de cancer secondaire apres allogreffe de cellules souches hematopoietiques? ; 2) quels sont les cancers plus frequents chez les survivants d'une allogreffe par rapport a la population generale? ; 3) quels sont les facteurs de risque de la survenue des cancers secondaires? ; et 4) comment peut-on prevenir l'apparition des cancers secondaires ?
The Cervical Spine. Ed. 3. The Cervical Spine Research Society Editorial Committee

The Cervical Spine. Ed. 3. The Cervical Spine Research Society Editorial Committee. Charles R. Clark, Chairman. Philadelphia, Lippincott-Raven, 1998. $179.00, 1003 pp. This book, produced by the Cervical Spine Research Society under the guidance of its Chairman, Charles R. Clark, M.D., comprises ten sections with seventy chapters written by 111 authors. The Editorial Committee has done an admirable job of coordinating a variety of specialists, including anatomists, bioengineers, neurologists, radiologists, orthopaedic surgeons, and neurosurgeons, to write the book. …
Deep venous thrombosis in patients with acute traumatic spinal cord injury: prevalence and patterns in a major teaching hospital in Uganda

Background: Spinal cord injured patients are at high risk of developing Deep Venous Thrombosis (DVT). This is because spinal cord injury favours two of the Virchow’s triad of factors for DVT; thus endothelial injury (trauma) and immobility (venous stasis). DVT and Pulmonary Embolism (PE) have long been known to be a major health problem in the West. However, the problem in Africa has been underestimated due to lack of data. Materials and methods : A cross sectional descriptive study was carried out at the spine unit of Mulago National Referral and Teaching Hospital from September 2012 to February 2013. Traumatic spinal cord injured patients who met the inclusion criteria were assessed for DVT using the model developed by Wells and colleagues. This included clinical assessment, D-dimer assay and Doppler sonography. Thromboprophylaxis was not given to any patient at any stage; however, treatment was instituted in those showing the features of DVT on investigations. Results: Out of 53 patients enrolled in the study, 48 were males and 5 were females. Most of the patients were in the age group of 21 to 40 years. DVT was found in 6 out of 53 patients representing 11.3%. All the patients who had DVT were in ASIA class A. There was bilateral involvement in one patient whereas two patients had thrombi in the left and three had thrombi in the right. A total of ten different thrombi were found, three proximal and seven distal. Clinical signs were found to be unreliable in the diagnosis of DVT in spinal cord injured patients. D-dimer test in combination with the Wells score was found to be a useful screening test and could reduce the need for further test if negative. Conclusion: We found a prevalence of 11.3% in the spinal cord injured patients. This prevalence was comparable to studies done in Asia but within the lower border of studies done in Caucasians. Distal DVT was more common as opposed to Caucasians where proximal DVT is more common. Clinical features of DVT were found to be unreliable in spinal cord injured patients due to the neurological injury. Keywords: Deep vein thrombosis, Pulmonary embolism, Traumatic spinal cord injury, Wells score, D-dimer assays, Doppler sonography EAOJ ; Vol. 7: September 2013
5-(5-Chloro-2-hydroxybenzoyl)-1-methyl-3-nitropyridin-2(1H)-one

In the title compound, C13H9ClN2O5, the dihedral angle between the planes of the benzene and pyridine rings is 55.30 (13)°. The nitro group is tilted by 38.21 (10)° with respect to the mean plane of the pyridine ring. In the crystal, mol­ecules are linked by O—H⋯O and C—H⋯O hydrogen bonds, forming a three-dimensional framework. The crystal packing is further stabilized by π–π stacking inter­actions [inter­centroid distance = 3.5877 (17) A].
2-[5-(4-Fluorophenyl)-3-(4-methylphenyl)-4,5-dihydro-1H-pyrazol-1-yl]-4-(5-methyl-1-phenyl-1H-1,2,3-triazol-4-yl)thiazole

The title compound, C28H23FN6S, comprises phenyl (A), triazolyl (B), thiazolyl (C), pyrazoyl (D), tolyl (E) and fluorophenyl (F) rings, with twist angles between neighbouring rings pairs A/B, B/C, C/D, D/E and D/F of 64.6 (1), 11.7 (2), 23.5 (2), 8.2 (2) and 73.3 (1)°, respectively. A short intramolecular C—H.N contact and a weak intermolecular C—H. π interaction occur. The crystal chosen for data collection was found to be an inversion twin.
Exploring decision making by occupational therapists in a school setting

Occupational therapists working in schools make many decisions regarding evaluation and services to support a student's special education. Six school-based occupational therapists from the Midwest shared, through interviews, how they chose assessment methods and tools for a particular student and how their evaluation findings were used to make service delivery decisions for that student. This group of therapists addressed team members' concerns, focusing on how sensory processing, handwriting, and functional skills influence a student's schoolwork performance. Their decisions regarding assessments were determined by the needs of the student and the questions raised by the educational team. Their evaluation information contributed to team decision-making regarding services and resulted in a variety of service delivery models. The results acknowledge the linearity of thinking used by school occupational therapists.
All Inorganic Colloidal Perovskite Quantum Dots: A New Class of Lasing Materials with Favorable Characteristics.

CsPbX3 (X: Cl, Br, I) quantum dots are prepared by injecting small amounts of a precursor solution consisting of Cs2CO3, oleic acid (OA), and octadecene (ODE) (150 °C, 1.5 h) to a solution comprising ODE, OA, oleylamine, and PbX2 at 160 °C.