Miracle-dz/UniFAR-FTUs huggingface dataset:
Efecto de oligofructosa de agave en dietas de gallinas ponedoras en la producción de huevos

Objective . To determine egg production in laying hens treated with oligofructose from agave. Materials and methods . Eighteen weeks old Hy-line W-36 hens (n=300) were distributed randomly into 3 treatment groups: no feed supplementation (control) or feed supplementation with 0.1% of 0.2% oligofructose from agave (OFA). Hens were monitored from development until 30 weeks of egg laying. Results . A significant (p<0.05) increase in the percent of egg-laying hens as well as increased in egg weight and egg quality occurred in hens from the OFA treatment groups relative to the control hens. Significantly lower levels (p<0.05) of fecal putrescine were observed in hens from the OFA treatment groups. Conclusions. The oligofructose from agave may be used as an alternative feed additive in laying hens.
The effects of undernutrition and dexamethasone treatment on cultured rat neonatal cardiomyocytes

It is well-established that undernutrition during pregnancy increases offspring’s risk of disease in later life. Studies have found that maternal protein restriction during rodent gestation programmes premature hypertrophic growth and decreased mitotic index of cardiomyocytes, as well as impaired contractile ability of the heart. All of these are predisposing factors to cardiovascular disease. To date, few studies have focused on the underlying mechanisms that lead to such alterations in the structure and function of the cardiovascular system. This study aimed to investigate if protein deficiency during gestation impacts upon cardiomyocyte proliferation, and glucose uptake and transport in vitro, and if programming effects can be demonstrated in culture. Methods. Trial 1: Ten pregnant wistar rats were fed either a control or low protein (MLP) diet throughout gestation. Pups were culled on the day of birth and hearts were taken to isolate cardiomyocytes. After 10 days in culture (baseline) some cardiomyocytes were treated with 100nM and 10μM of dexamethasone (Dex) for 48 hours (Day 2). Both untreated and Dex treated cultures were analysed for proliferation and differentiation rates, and glucose uptake. Trial 2: Gestational diet and culturing of cardiomyocyte cells was the same as in trial one. Cultures were treated with 10nM and 100nM Dex and analysed at the same time points for proliferation and differentiation rates, cell apoptosis and mRNA expression of glucose transporters. Results. In trial one, MLP diet increased day 2 binucleation. High concentrations of Dex increased insulin stimulated glucose uptake in MLP neonatal cardiomyocytes indicating they had an increased sensitivity to glucocorticoids. In trial two, Dex treatment decreased expression of GLUT 4 and increased expression of glucocorticoid receptor (GR) mRNA. Conclusion. Results indicate possible remodelling of the heart in rat offspring exposed to MLP diet in utero, but it remains unclear as to what mechanism underpins MLP cultures increased sensitivity to glucocorticoids. There were some similarities to the findings of in vivo studies, but responses varied between trials. This indicates that cell culture is unlikely to become a viable alternative model, but it is a useful tool for exploring focused mechanisms of fetal programming.
The Cultural Stress Theory of Obesity

The characteristics of modern society contribute to a condition we call Cultural Stress Anxiety Syndrome (CSAS), which is a response to the constant, pervasive, ever-increasing stress of modern living. Cultural Stress often leads to an isolated, sedentary lifestyle and a state of constant anxiety that triggers the body’s inflammation response, including the release of stress hormones linked to weight gain. Because Cultural Stress aggravates all other causes of obesity, it must be included in any effective weight management program. We present a review of the factors implicated in the obesity epidemic, as well as the implications of chronic stress on a variety of health issues. We then outline the evidence for Cultural Stress as a phenomenon that compounds other stressors, as well as the societal factors most responsible for Cultural Stress. We report on an initial pilot study that focuses on Cultural Stress-reduction as part of an inclusive health management program. We conclude that Cultural Stress is a factor in virtually all of the conditions that contribute to obesity: higher stress hormones, disrupted sleep, sedentary lifestyle, isolation, and reduced self-esteem. Supporting patients in managing Cultural Stress empowers them to effectively reduce their weight and maintain that weight loss over time.
Analyses of the roles of the UmuDC proteins of E. coli in SOS mutagenesis and cell cycle regulation

Regulated mechanisms that inhibit DNA synthesis and cell cycle progression in response to DNA damage have been shown to be essential for DNA damage tolerance in eukaryotes. Analogous mechanisms have not been as well defined in prokaryotes. Evidence presented in this thesis suggests that the UmuD and UmuC proteins of Escherichia coli participate in a mechanism to inhibit growth and DNA synthesis upon exposure to DNA damaging treatments, thereby increasing cell survival. This hypothesis grew out of the study of umuDC-mediated growth inhibition at 30°C (cold sensitivity). The umuDC operon is a member of the SOS regulon, a group of twenty or more genes whose expression is coordinately induced by DNA damaging treatments. The UmuD' and UmuC proteins have been well characterized for their activity in SOS mutagenesis, the mutagenesis resulting from exposure to DNA damaging agents such as UV irradiation. Constitutive expression of the umuDC operon from a multicopy plasmid confers cold sensitivity for growth which is associated with an inhibition of DNA synthesis. The genetic requirements for observing umuDC-mediated cold sensitivity are distinct from those for SOS mutagenesis supporting that these are distinct functions of the umuDC gene products. High levels of intact UmuD, the form inactive in SOS mutagenesis, confer growth inhibition at 30°C in combination with UmuC. Further analyses revealed that the umuDC gene products, expressed at physiologically relevant levels, inhibit the transition from stationary phase to exponential growth of UV irradiated stationary phase cells and increase UV resistance, possibly by allowing time for DNA repair processes to be completed. This is most likely the result of the inhibition by the umuDC gene products of a Fis-dependent activity. Suppression of the UV sensitivity of a AumuDC strain by afis mutation demonstrates that the inhibition of afis-dependent activity is central to the UV resistance conferred by the umuDC gene products. Intact UmuD and UmuC increase cell survival after UV irradiation. This previously uncharacterized activity of UmuD and UmuC is correlated with the inhibition of DNA synthesis after UV irradiation. The umuC125 mutation, which specifically interferes with the ability of UmuC to confer growth inhibition at 30'C without interfering with SOS mutagenesis, also abolishes the ability of UmuC to regulate DNA synthesis after UV irradiation. This suggests that this novel activity of intact UmuD and UmuC is functionally related to umuDC-mediated growth inhibition at 30°C. Thesis supervisor: Graham C. Walker Title: Professor of Biology
Parasitic helminths : targets, screens, drugs and vaccines

parasitic helminths targets screens drugs and vaccines ebook download parasitic helminths targets screens drugs part one targets wiley-vch parasitic helminths download.e-bookshelf ebook download parasitic helminths targets screens drugs parasitic helminths targets screens drugs and vaccines 3 integrating and mining helminth genomes to discover and parasitic helminths targets screens drugs and vaccines document about spiritual terrorism is available on print a scenic route to infectiology drug discovery wiley: home guyton and hall textbook of medical physiology 12e ebook diagnostics a. neb basic icd 10 cm and icd 10 pcs coding 2017 ebook | gink new publications sage pub rediscovering passovera complete guide for christians why intelligent people are overweight nownfc 18 discovery, mode of action, and commercialization of volume 5 protein phosphorylation in parasites 2012 december, voltune 46 theannals sage pub help lord im having a senior momentnotes to god on growing the end is nigh a history of natural disasters aadver heart disease everything you need to know your personal health document about phytosfere99 highlights in european plant crawford county ohio court records laudit thinking in new boxesa new paradigm for business nothing rhymes with orange perfect words for poets download official liverpool fc book records boscos easy company and the gypsy riders no 29 heeng journalal anon slogans journal 6x9lightly lined 160 pages
KECANDUAN SOSIAL MEDIA DITINJAU DARI PERBEDAAN GENDER

Berkembangnya teknologi membuat perkembangan arus informasi juga mengalir dengan cepat. Salah satu tempat mengalirnya informasi yaitu sosial media. Jumlah pengguna sosial seperti facebook, twitter, instagram, whatsapp, dan lainnya terus bertambah sehingga berakibat pada kecanduan dalam menggunakan sosial media. Tujuan penelitian ini untuk meneliti apakah terdapat perbedaan tingkat kecanduan sosial media ditinjau dari jenis kelamin. Subjek penelitian berjumlah 85 Mahasiswa dari Universitas Muhammadiyah Malang. Instrumen penelitian yang digunakan yaitu social media disorder scale. Teknik analisis data dalam penelitian ini menggunakan analisis t-test. Hasil peneliitian ini menunjukkan bahwa terdapat perbedaan yang signifikan antara mahasiswa laki-laki dan perempuan dalam tingkat kecanduan sosial media (M: Laki-laki, 20,79; Perempuan, 23,81).
TaReCa - Kaskadennutzung gartenbaulicher Biomasse für eine ressourceneffiziente Produktion von wertvollen bioaktiven Substanzen

Viele pflanzliche Sekundarmetabolite haben antioxidative oder andere bioaktive Eigenschaften, weshalb sie einerseits wichtige Bestandteile der menschlichen Ernahrung sind, andererseits aber auch als pharmazeutische Verbindungen oder als Substrat fur die chemische Synthese von bioaktiven Substanzen verwendet werden. Pflanzen induzieren die Produktion solcher nutzbaren Sekundarmetabolite wie z.B. Flavonoiden als Reaktion auf abiotischen Stress. Die Produktion von Gemuse und Fruchten in Gewachshausern hinterlasst grose Mengen an ungenutzter pflanzlicher Biomasse, welche eine potentielle Ressource fur die Gewinnung wertvoller Metabolite darstellt. Durch eine kaskadenartige Verwendung von Gartenbaukulturen zur Produktion von Fruchten und Gemuse mit einer anschliesenden Gewinnung hochwertiger Substanzen aus der verbleibenden Restbiomasse wurde ein erheblicher Mehrwert generiert. Das Projekt TaReCa bearbeitet die Entwicklung einer masgeschneiderten Kaskadenverwertung von Paprikapflanzen-Restbiomasse aus dem Gartenbau. Dabei soll der pflanzliche Sekundarmetabolismus durch spezifische abiotische Stressbedingungen nach der Fruchternte gezielt induziert werden, um die Konzentrationen der Zielmetaboliten zu steigern. Durch umweltfreundliche und wirtschaftliche Extraktionsprozesse und eine anschliesende Verwertung des verbleibenden Pflanzenmaterials in einer Bioraffinerie wird die Wertschopfungskette erweitert. Eine Analyse der Anwendungsgebiete sowie Untersuchungen zur Akzeptanz der induzierten Inhaltsstoffe, Prozesse und Technologien werden helfen, das Marktpotenzial der Restbiomasse fur die Nutzung in Kaskaden zu evaluieren. Die masgeschneiderte Nutzung von Gartenbaubiomasse durch Lebensmittelproduktion, Extraktion bioaktiver Sekundarmetabolite und Bioraffinerien kann wirtschaftlich relevante, biobasierte Produkte fur industrielle Anwendungen erzeugen und somit zur Entwicklung einer nachhaltigen, effizienten und integrierten Biookonomie beitragen, ohne mit der Lebensmittelproduktion zu konkurrieren.
Epstein-Barr virus latent proteins regulate expression of the anti-apoptotic cellular bfl-1 gene

The ubiquitous and oncogenic human herpes-virus Epstem-Barr virus (EB V) establishes a latent infection and promotes the long-term survival of the infected host cell by targeting the molecular machinery that controls cell fate decisions, including apoptosis, proliferation and differentiation. These host-virus interactions are likely to play a crucial role in the development of EBV-associated malignancies such, as Burkitt’s lymphoma, Hodgkin’s disease, nasopharyngeal carcinoma and tumours in lmmunosuppressed individuals. It has previously been shown in our laboratory that two EBV latent proteins, latent membrane protein 1 (LMP1) and EBV nuclear antigen 2 (EBNA2), which are major effectors of cellular phenotypic change, can independently regulate expression of the cellular bfl-1 gene Bfl-1 is an anti-apoptotic protein of the Bcl-2 family, whose preferential expression in hematopoietic and endothelial cells is controlled by inflammatory stimuli. In this study, it is reported that LMP1 and EBNA2 regulate bfl-1 activity through interactions with components of the NF-kB and Notch signalling pathways respectively NF-kB composed of p65 sub-units trans-activated the bfl-1 promoter m the EBV-negative cell line DG75, and an NF-icB-like binding site at position -52 to -43 relative to the transcription start site was essential for this effect. An RBP-Jk/CBF1 mutant blocked EBNA2-mediated trans-activation of bfl-1 in DG75 cells, indicating an important role for this DNA-binding protein in bfl-1 trans-activation by EBNA2. Although RBP-Jk/CBFI is also essential for signalling by the cellular equivalent of EBNA2, mtra-cellular Notch (NotchIC), this protein was not found to trans-activate the bfl-1 promoter. Both EBNA2 and LMP1 are expressed in EBVmfected cell lines, and EBNA2 is responsible for induction of LMP1 Blocking of either EBNA2- or LMP1-mediated signalling in EBV-mfected cell lines did not dramatically affect the level of bfl-1 promoter activity. However, when both EBNA2 and LMP1 signalling were blocked simultaneously, a significant decrease in the level of bfl-1 activity was observed. These data indicate a role for both EBNA2 and LMP1 in the regulation of the promoter for the bfl-1 gene m the context of the EBV-infected cell. These findings are relevant to our understanding of EBV persistence in the infected host, and its role in malignant disease.
The Reaction of 2-Methyl-1, 4-naphthoquinone with Sulfhydryl Compounds

1. 2-Methyl-1, 4-naphthoquinone (K3) reacted with sulfhydryl compound to form a thioether linkage at 3-position of K3 with an absorption maximum at 420-430mμ. 2. For this reaction, molecular oxygen and alkaline metal ions such as Na+ and K+ were essential. One half mole of oxygen was consumed for the reaction of one mole of K3 with one mole of sulfhydryl compound. 3. The reaction product of K3 with cysteine was unstable and gradually turned to the insoluble amorphous polymer. By methylation of intermediate, it was identified to be methyl 5-methyl-6-methoxy-3H-naphtho [2, 1-b] [1, 4]-thiazine-2-carboxylate.
In-vitro antibacterial activity of Thonningia sanguinea [Balanophoraceae (Vahl)] flowers extracts

The aim of the present study was to investigate antimicrobial activity of the various extracts of Thonningia sanguinea leaves. Alkaloids and water extracts of Thonningia sanguinea leaves were investigated against Escherichia coli ATCC25922, Salmonella paratyphi A. , Salmonella typhi and Enterobacter aerogenes by using agar disc diffusion method and tube dilution. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined. All the extracts had bactericidal activity against the microorganisms used in the study. But Water extract showed the best activity with the MIC ranging from 1,56 to 6,25 mg/mL and the CMB from 3,125 to 12,5 mg/mL. The study confirms the possible antimicrobial potentiality of the leaf extract of Thonningia sanguinea. Keywords: Thonningia sanguinea, antimicrobial activity, flowers extracts, Cote d'Ivoire
Prevalence of Salmonella in diarrheic adult goats in field conditions.

An active surveillance was undertaken to determine the prevalence of Salmonella from the diarrheic feces of adult goats, in field conditions. Salmonella is a principal cause of food-borne diseases in the world, and the most common zoonotic bacterial disease of adult goats. In this long study of one year, a total of 744/2029 cases of diarrhea were identified, which revealed an overall prevalence of diarrhea in goats to be 36.7% and salmonellosis 0.1%, with the help of commercially available API 20E kits. A significant association of a number of cases of diarrhea with the environmental temperature and humidity level of the area was established but there was no effect of season, temperature or humidity levels with the prevalence of salmonellosis in the area. Open grazing was a common practice and the animals would eat garbage, grass, leaves and drink standing water, available to them. The detection of Salmonella species in feces, from field conditions, needs special attention to prevent an outbreak in animal and human population in the area.
Cell cycle regulation of septins: implications for cytokinesis and the spindle position checkpoint

9 RIASSUNTO.13 INTRODUCTION.18 o Asymmetric cell division and spindle positioning o Regulation of mitotic exit o Regulation of cytokinesis o Septins in eukaryotes o Septins in budding yeast o The mitotic checkpoints o The spindle position checkpoint o The morphogenesis checkpoint o Dma proteins in S. cerevisiae RESULTS.63 o Dma proteins control septin ring deposition and maintenance o The FHA and RING domains are required for Dma proteins’ function in septin ring deposition and stabilization o The lethal effects of DMA2 overexpression are suppressed by septin ring destabilization o dma1 dma2 cla4 lethality is suppressed by RTS1 deletion o Simultaneous lack of Dma1, Dma2, Cla4 and Swe1 causes spindle mispositioning without activation of the spindle position checkpoint o Dma1 and Dma2 control the SPOC independently of Bub2/Bfa1 o Dma1 and Dma2 control the bud neck localization of the Elm1 kinase o A genetic screen to find extragenic suppressors of the lethality of dma1 dma2 cla4 cells o MUT-135 is mutated in RHO1
Functional Analysis of Yeast Pheromone Receptors in ER Exit, Ligand-Induced Endocytosis and Oligomerization: A Dissertation

This study investigates endocytosis and ER export signals of the yeast ! -factor receptor and the role that receptor oligomerization plays in these processes. The ! -factor receptor contains signal sequences in the cytoplasmic C-terminal domain that are essential for ligand-mediated endocytosis. In an endocytosis complementation assay, I found that oligomeric complexes of the receptor undergo ligand-mediated endocytosis when the ! -factor binding site and the endocytosis signal sequences are located in different receptors. Both in vitro and in vivo assays strongly suggested that ligand-induced conformational changes in one Ste2 subunit do not affect neighboring subunits. Therefore, the recognition of endocytosis signal sequence and the recognition of the ligand-induced conformational change are likely to be two independent events, where the signal sequence plays only a passive role in the ligand-induced endocytosis. Four amino acid substitutions (C59R, H94P, S141P and S145P) in TM domains I, II and III were identified that resulted in the accumulation of truncated receptors in the ER but did not block ER export of full-length receptors. The two DXE motifs in the C-terminal tail were required for export of the mutant receptors from the ER; however DXE was not essential for proper cell surface expression of wild-type receptors apparently because the receptors contain redundant ER export signals. An assay for oligomerization of receptors in the ER was developed based on the ability of truncated mutant receptors to exit the ER. The four substitutions (C59R, H94P, S141P and S145P) that caused DXE-dependent ER export failed to form homo-oligomers, suggesting that the DXE motifs and receptor
Analysis of protein trafficking in the yeast Saccharomyces cerevisiae

The yeast ABC-transporter Ste6 is rapidly internalized from the cell surface and transported to the vacuole. Trafficking to the vacuole is regulated by ubiquitination. However, the ubiquitin machinery involved is still unknown. Rsp5 is a HECT E3 ubiquitin ligase implicated in ubiquitination of many membrane proteins. Therefore the role of Rsp5 in Ste6 trafficking and ubiquitination was examined. We found that Ste6 trafficking was indeed affected in the rsp5-1 mutant. The Ste6 protein was no longer delivered to the vacuole, but instead accumulated at the vacuolar membrane. However, Rsp5 was not the E3 ligase for the Ste6 protein, since Ste6 ubiquitination was not affected in the rsp5-1 mutant, suggesting that the observed effects are indirect. Indeed, we found that free ubiquitin levels were reduced in this mutant, indicating that this could be the problem in the rsp5-1 mutant. It appears that reduced synthesis of ubiquitin contributes to ubiquitin depletion. A transient inhibition of protein synthesis is observed in wildtype upon heat-shock. And, while wildtype cells recover quickly from this transient arrest, the rsp5-1 cells remain arrested, suggesting that Rsp5 is important for recovery from heat-induced protein synthesis arrest. In addition, the role of ubiquitination on Ste6 trafficking was studied. The distribution of Ste6 variants with reduced ubiquitination was examined. We found evidence that ubiquitination controls Ste6 sorting in the early endocytic pathway. While wildtype Ste6 was mainly localized to internal structures, ubiquitination deficient Ste6 variants accumulated at the cell surface in a polar fashion. However, in a recycling deficient ypt6-2 mutant, these Ste6 variants were trapped inside the cell, indicating that their polar localization is achieved through endocytic recycling and localized exocytosis ("kinetic polarization"). Furthermore, it appears that Ste6 cycles through early endosomes, since its recycling was partially affected in the sorting nexin mutant snx4? The identification of putative recycling signals in Ste6 suggests that recycling is a signal mediated process. Endocytic recycling and localized exocytosis could be important for Ste6 polarization during the mating process.
Isolation and identification of Pseudomonas fluorescens in tilapia.

One pathogenic gram-negative bacillus bacterial strain with β-hemolysis properties was isolated from the sick tilapia collected from the fish-breeding pond in Guangxi.The isolate was identified as Pseudomonas fluorescens on the basis of morphology,cultural performances and biochemical characteristics.The results were verified by PCR diagnosis,nucleotide sequencing and NCBI blast.The results of the pathogenicity test showed that the isolate had strong pathogenicity to tilapia and induced morbidity and mortality with similar pathological change in red skin disease of fish induced by Pseudomonas fluorescens.The results of drug sensitivity showed that the isolate was highly sensitive to norfloxacin,ofloxacin and neomycin,but insensitive to ampicillin,penicillin and amoxicillin,etc.
Factor influencing sex ration in Brown swiss cattle

Data for the birth records were collected from Brown Swiss cows raised at the Regional Agricultural School in Van, Tiirkiye. The sex ratio (% male birth) was 0.55±0.06. Season of birth had nonsignificant effect on sex ratio, but there were some differences between seasons with sex ratios ranging from 0.45±0.09 (autumn) to 0.61±0.06 (winter). Parity had nonsignificant effect on sex ratio, but there were some differences between parity with sex ratios ranging from O.46±O.08 (first) to O.70±0.27 (ninth). Year of birth had nonsignificant effect on sex ratio, but there were some differences between parity with sex ratios ranging from O.80±O.36 (1969) to O.35±O.11 (1981). The heritability of sex ratio was found to be O.056±O.079 and O.045±0.002, whereas repeatability was 0.066.