Miracle-dz/UniFAR-FTUs huggingface dataset:
[Isolation and characterization of human prostate cancer cell subclones with different metastatic potential].

OBJECTIVE To isolate and characterize cancer cell subclones with different metastatic potential from human metastatic prostate cancer cell line (PC-3M). METHODS Using limited dilution, in vitro growth, Matrigel invasion assay, soft agar cloning and in vivo tumorigenicity and spontaneous metastasis assay in nude mice, were isolated and characterized four subclones with different metastatic potential. RESULTS Four subclones derived from PC-3M were 1E8, 2E7, 2B6, 2B4. Each subclone exhibited a different metastatic potential when inoculated into nude mice. Among these subclones, 1E8 expressed as the highly metastatic phenotype, with 100% metastasis frequency 5 weeks after subcutaneous inoculation into nude mice, whereas 2B4 was not metastatic. In vitro 1E8 was found to be the most highly invasive cell line in Matrigel invasive assay (98 +/- 24) and had the most clones in soft agar cloning assay (265 +/- 39) while 2B4 was found to have the least invasive abilities (12 +/- 4) and the least clones (137 +/- 14). CONCLUSION Successful establishment of these subclones with different metastatic potential may be valuable for further study on the molecular mechanisms of cancer metastasis and cloning of cancer metastasis-related genes.
An acidic sequence of a putative yeast Golgi membrane protein binds COPII and facilitates ER export

We previously identified Sys1p as a high copy number suppressor of Ypt6 GTPase‐deficient yeast mutants that are defective in endosome‐to‐Golgi transport. Here, we show that Sys1p is an integral membrane protein that resides on a post‐endoplasmic reticulum (ER) organelle(s). Affinity studies with detergent‐ solubilized yeast proteins showed that the C‐terminal 53 amino acid tail of Sys1p binds effectively to the cytoplasmic Sec23p–Sec24p COPII subcomplex. This binding required a di‐acidic Asp‐Leu‐Glu (DXE) motif, previously shown to mediate efficient ER export of the vesicular stomatitis virus glycoprotein in mammalian cells. In Sys1p, a Glu‐Leu‐Glu (EXE) sequence could not substitute for the (DXE) motif. Mutations of the (DXE) sequence resulted in ER retention of ∼30% of the protein at steady state, whereas addition of the Sys1p tail to an ER‐resident membrane protein led to an intracellular redistribution of the chimeric protein. Our study demonstrates for the first time that, in yeast, a di‐acidic sequence motif can act as a sorting signal for cargo selection during the formation of transport vesicles at the ER by direct binding to COPII component(s).
Research at the Armed Forces Research Institute of Medical Sciences , Bangkok , Thailand

The Anned Forces Research Institute of Medical Sciences (AFRIMS) in Bangkok, Thailand was established in 1960. The Department of Virology has a long tradition of tlavivirus research that started with studies on dengue haemorrhagic fever (IIHF). AFRIMS has contributed to tlavivirus research with observation5 on the epidemiology of dengue and Japancsc encephalitis (JE) and the developnlent of key assays to test for these viral pathogens. Notable achievements were the developrrient of the plaque recluctio~l ne~~tralisation assay and the IgMflgC; enLyme immuno-assay Sor JE and dengue as well as the JE vaccine trial in Thai children. The Department oS Virology, AFKIMS. continues thi\ strong tr.adition of tlavivirus rcsearch iniliafed in thc 1960s. Today, scientific PI-otocols are being performed with both Thai and international coll;rboralors on the i~nrnunology of DHF, the pathogcncsis of asymptomatic dengue disease, the long-term circulation of dengue serotypes in Thailand and vaccine developnlent of a tetravalent dengue vaccine. Japanese encephaliti\ is an additional important focus of our tlavivirus research. Aclive research protocols are ongoing on the epidemiology and cl~nical manifestations of JE, the occurrence of natural JE inkction and irnrnunologic boosting in children vaccinated with the killed JE vaccine and the development of new second gcneratlon JE vaccines. The Department maintains a high level of expertise and is a resource on flaviviru5 diagnostics. the epidemiology and immunology of flavivirus diseases in Southeast Asia, and tlavivirus vaccine development. THE UNITED States Army Medical Component, Armed Forces Research Institute of Medical Sciences (USAMC-AFRIMS) has a long and distinguished history of research in tropical infectious diseases. Established in 1958 as part of the Southeast Asian Treaty Organization (SEATO). USAMCAFRIMS has been instrumental in documenting and reporting the emergence of dengue and dengue haemorrhagic fever, Japanese encephalitis, chikungunya, hepatitis A, hepatitis E, cholera, antibioticresistant enteric organisms, drug-resistant malaria, drug-resistant scrub typhus and HIV virus (subtype E). Its current extensive network of field sites throughout Southcast and Southwest Asia continues to provide essential infornlation on these diseases as well as potential emerging new infectious diseases. ' Chief, Department of Virology, AI-med Forces Research Institute of Medical Sciences. 31516 Rajvithi Road, Bangkok, 10300, Thailand Epidemiology of Dengue in Thailand Dengue virus is the causative agent of dengue fever (DF) and dengue haemorrhagic fever (DHF) and has emerged in the last 50 years as one of the most important global health problems of the 20th century. In Thailand, the reported nunhe r of cases of dengue disease has increased since 1958 to more than 5 0 000 cases of DHF and D F per year, with periodic outbreaks of more than 100 000 cases. Little is known about the long-term circulation of dengue serotypes and their association with epidemics and severe dengue disease in a country hyperendemic for
A Unifying Genetic Model for Facioscapulohumeral Muscular Dystrophy

Addition by Contraction Facioscapulohumeral muscular dystrophy (FSHD) is one of the most common hereditary neuromuscular disorders in Western populations, affecting about 1 in 20,000 people. In most patients, the disorder is associated with contraction of a D4Z4 microsatellite repeat array on chromosome 4q, but this contraction can also occur in the absence of disease, so the underlying genetic mechanisms have remained elusive. Lemmers et al. (p. 1650, published online 19 August; see the Perspective by Mahadevan) now show that FSHD patients carry sequence variants that create a canonical polyadenylation signal for transcripts derived from DUX4, a homeobox gene straddling the last D4Z4 repeat unit and the adjacent sequence. Addition of poly(A) stabilizes the DUX4 transcript, which is likely to be a contributing factor in the disease. Sequence variants shared by patients with a genetically complex form of muscular dystrophy explain how the disease arises. Facioscapulohumeral muscular dystrophy (FSHD) is a common form of muscular dystrophy in adults that is foremost characterized by progressive wasting of muscles in the upper body. FSHD is associated with contraction of D4Z4 macrosatellite repeats on chromosome 4q35, but this contraction is pathogenic only in certain “permissive” chromosomal backgrounds. Here, we show that FSHD patients carry specific single-nucleotide polymorphisms in the chromosomal region distal to the last D4Z4 repeat. This FSHD-predisposing configuration creates a canonical polyadenylation signal for transcripts derived from DUX4, a double homeobox gene of unknown function that straddles the last repeat unit and the adjacent sequence. Transfection studies revealed that DUX4 transcripts are efficiently polyadenylated and are more stable when expressed from permissive chromosomes. These findings suggest that FSHD arises through a toxic gain of function attributable to the stabilized distal DUX4 transcript.
Stochastic simplified modelling of abrasive waterjet footprints

Abrasive micro-waterjet processing is a non-conventional machining method that can be used to manufacture complex shapes in difficult-to-cut materials. Predicting the effect of the jet on the surface for a given set of machine parameters is a key element of controlling the process. However, the noise of the process is significant, making it difficult to design reliable jet-path strategies that produce good quality parts via controlled-depth milling. The process is highly unstable and has a strong random component that can affect the quality of the workpiece, especially in the case of controlled-depth milling. This study describes a method to predict the variability of the jet footprint for different jet feed speeds. A stochastic partial differential equation is used to describe the etched surface as the jet is moved over it, assuming that the erosion process can be divided into two main components: a deterministic part that corresponds to the average erosion of the jet and a stochastic part that accounts for the noise generated at different stages of the process. The model predicts the variability of the trench profiles to within less than 8%. These advances could enable abrasive micro-waterjet technology to be a suitable technology for controlled-depth milling.
Measurement based ray tracer calibration and channel analysis for high-speed railway viaduct scenario at 93.2 GHz

In this paper, ray tracer (RT) is employed to simulate the propagation in a high-speed railway viaduct scenario at the frequency of 93.2 GHz. By comparing the path loss between simulation and measurement, the permittivity and loss tangent of concrete which is the material of viaduct are calibrated in the ray tracer. The average error between calibration result and measurement is 0.2 dB. The calibrated and validated RT is used to study propagation channel in viaduct scenarios. Rician K-Factor and root-mean-square (RMS) delay spread are further analyzed, from which a conclusion that the typical structure of viaduct and the application of horn antenna lead to a small value of Rician K-Factor and RMS delay spread is drawn.
Investigación de virus respiratorios en niños menores de dos años hospitalizados por infección respiratoria aguda baja

Las infecciones respiratorias agudas bajas son causa frecuente de internación en los primeros años de vida, constituyendo un tema prioritario por su gran implicancia en la morbimortalidad infantil. Esto lleva a la necesidad de disponer de mayor información sobre esta enfermedad, que permita desarrollar estrategias de asistencia, mejorando la eficiencia de los recursos de salud. Entre el 1 de mayo y el 3 de noviembre de 1998, se estudiaron 182 niños menores de dos años ingresados en las clínicas pediátricas del Centro Hospitalario Pereira Rossell, con diagnóstico de infección respiratoria aguda baja de probable etiología viral. Se obtuvieron muestras de aspirados nasofaríngeos para investigación de antígenos virales por inmunofluorescencia indirecta. La inmunofluorescencia fue positiva en el 53,3% de los casos. Predominó el virus respiratorio sincicial (83,5%),seguido por influenza A (6,2%) y adenovirus (5,2%), encontrándose cuatro casos que asociaban virus respiratorio sincicial e influenza (4,1%) y un caso de adenovirus y virus respiratorio sincicial (1,0%). El grupo de niños con inmuno-fluorescencia positiva se comparó con los que tuvieron inmunofluorescencia negativa, no se hallaron diferencias estadísticamente significativas en ninguno de los aspectos comparados (hallazgos radiológicos, utilización de antibióticos y otros). Existieron cuatro casos de infección intrahospitalaria. No se registraron fallecimientos. Se destaca la importancia de contar con una técnica de diagnóstico virológico rápida, sensible y específica, en la cual se pueda basar la elaboración de un programa de internación, diagnóstico y tratamiento que permita racionalizar el uso de antibióticos y reducir la tasa de infecciones intrahospitalarias.
CONSERVED CELLULAR AND MOLECULAR MECHANISMS IN DEVELOPMENT

This review discusses examples of conserved cellular and molecular mechansims in development, including the pathway of signal transduction between the photoreceptors R8 and R7 in Drosophila, which is compared to vulval induction in Caenorhabditis elegans. The Wg pathway in Drosophila is compared, first, to theWnt pathway in dorsal mesoderm specification in Xenopus; second, to the same pathway in sea urchins; third, to the equivalent in the mom cascade of C. elegans; and finally, to parts of the equivalent pathway inDictyostelium discoideum. The conserved expression of some hox genes in vertebrate limb buds and in the heads or tails of several invertebrate and vertebrate embryos is also illustrated. Two further examples show the contrast between BMP4 and the proteins Noggin, Chordin, Follistatin and Gremlin. Another example concerns the binding of proteins to the 3′UTRs of several messages which inhibits their translation in Drosophila and C. elegans. The final example illustrates the selective transfer of transcription factors to nuclei in Drosophila and C. elegans.
Social Anxiety Disorder

Social anxiety disorder (SAD) is characterized by excessive fear of embarrassment, humiliation, or rejection when exposed to possible negative evaluation by others when engaged in a public performance or social interactions. It is also known as social phobia. Over fifty years ago, in 1966, social phobia was first differentiated from agoraphobia and specific phobias. Since that time, the concept has transformed from being a relatively rare and neglected condition to one that is recognized as prevalent throughout the world. The third edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-III) in 1980 described social phobia in a way that limited the diagnosis due to exclusionary criteria including those with an avoidant personality disorder, a new category at the time. In 1985 that view was challenged, and by 1987 the DSM-III-R removed the exclusion. In 1994, DSM-IV added the alternative name of SAD, due to a recognition that social phobia could be differentiated from specific phobias due to important pathophysiological and clinical factors. With the publication of DSM-5 in 2013, SAD became the primary name. With the publication of DSM-5, the diagnostic criteria for SAD have been broadened from previous editions to include fear of acting in a way or show anxiety symptoms that offend others or lead to rejection in addition to fear of humiliation or embarrassment. Additionally, the latest edition of DSM removed the generalized subtype and added the "performance only" specifier.
Developing new sensing capabilities for archaeological operations using wideband sonar systems

In this paper, we present some of the results of the Tallinn trials. The trials were part of the ARROWS (ARchaeological RObot systems for the World's Seas) FP7 project whose aims are to reduce the cost of the AUVs and sensors in order to generalize their use for underwater archaeological missions. To this end, we developed algorithms to improve the imagery resolution of wideband sonar systems with wide beamwidth. We discuss in this paper an incoherent synthetic aperture algorithm and present early results of such method.
Glyceraldehyde-3-Phosphate Dehydrogenase of Streptococcus pneumoniae Is a Surface-Displayed Plasminogen-Binding Protein

ABSTRACT The recruitment of plasminogen endows the bacterial cell surface of Streptococcus pneumoniae with proteolytic activity. In this study we demonstrate specific plasmin- and plasminogen-binding activity for the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which is located in the cytoplasm as well as on the surface of pneumococci. GAPDH exhibits a high affinity for plasmin and a significantly lower affinity for plasminogen.
Detection of the Characteristic Pion-Decay Signature in Supernova Remnants

Accelerated Protons Although cosmic rays were first detected a hundred years ago, their origin is still not fully understood. Comic rays are high-energy particles, mostly protons, which bombard Earth from outer space. Most of those that originate from within our galaxy are thought to be accelerated in the shock waves from the explosion of massive stars, or supernovae. Protons accelerated in a supernova remnant will collide with interstellar material producing pions, a type of subatomic particle, which will then decay into gamma rays. Ackermann et al. (p. 807) present measurements of the energy spectra of two supernova remnants in our galaxy, IC443 and W44, in the energy range below 100 MeV down to 60 MeV with the Fermi Gamma-Ray Space Telescope. The spectra reveal the pion-decay feature that is a signature of accelerated protons. The gamma-ray spectra of two supernova remnants in our Galaxy show the signature of proton acceleration. Cosmic rays are particles (mostly protons) accelerated to relativistic speeds. Despite wide agreement that supernova remnants (SNRs) are the sources of galactic cosmic rays, unequivocal evidence for the acceleration of protons in these objects is still lacking. When accelerated protons encounter interstellar material, they produce neutral pions, which in turn decay into gamma rays. This offers a compelling way to detect the acceleration sites of protons. The identification of pion-decay gamma rays has been difficult because high-energy electrons also produce gamma rays via bremsstrahlung and inverse Compton scattering. We detected the characteristic pion-decay feature in the gamma-ray spectra of two SNRs, IC 443 and W44, with the Fermi Large Area Telescope. This detection provides direct evidence that cosmic-ray protons are accelerated in SNRs.
Wearable, Small, and Robust: The Circular Quarter-Mode Textile Antenna

A miniaturized wearable antenna, entirely implemented in textile materials, is proposed that relies on a quarter-mode substrate integrated waveguide topology. The design combines compact dimensions with high body-antenna isolation, making it excellently suited for off-body communication in wearable electronics/smart textile applications. The fabricated antenna achieves stable on-body performance. A measured on-body impedance matching bandwidth of 5.1% is obtained, versus 4.8% in free space. The antenna gain equals 3.8 dBi in the on-body and 4.2 dBi for the free-space scenario. High radiation efficiency, measured to be 81% in free space, is combined with a low calculated specific absorption rate of 0.45 mW/g, averaged over 1 g of tissue, with 500 mW input power.
Technical Analysis of the Taiwanese Stock Market

We study the profitability of technical trading rules based on 9 popular technical indicators. To further examine whether investors can design technical trading strategies that can beat the buy-and-hold strategy, we establish 13 trading models based on one indicator, 25 models based on two indicators, and 28 models based on three indicators. The empirical results show that 58 out of 66 models reject the null hypothesis of equality of the mean returns between buy days and sell days. Our findings provide support for the predictive power of technical trading rules. Finally we employ Hansen’s (2005) Superior Predictive Ability to investigate data snooping problem. Overall we observe an inverse association between the number of technical indicator combinations and trading profitability.
Do primary stroke centers occur randomly?

See related article, p 3717. When the Brain Attack Coalition began to publish recommendations for the development of Stroke Centers, they chose to begin with primary stroke centers (PSCs), in the hope that such centers would impact the largest number of patients in the shortest period of time.1,2 Currently, in the United States, there are >1000 PSCs certified by the Joint Commission, and likely several hundred more certified by other private agencies (ie, Healthcare Facilities Accreditation Program and Det Norske Veritas), as well as some state health departments. Considering there are slightly >5000 acute care hospitals in the country, this means that ≈25% of hospitals in the United States are a PSC. This represents a significant advance in stroke care over a little more than a decade since the Joint Commission began their PSC certification program. As McDonald and colleagues note,3 PSCs are associated with many improved important outcomes (reduced death and disability, higher rates of utilization of intravenous tissue-type plasminogen activator), as well as better compliance with several other stroke care measures as tracked by Get with the Guidelines-Stroke.4,5 It is now common, if not routine, for some cities and some regional emergency medical service providers to divert patients with a suspected acute stroke to the nearest PSC or Comprehensive …
Performance of hybrid subcarrier multiplexed — optical CDMA system based on different detection methods

In this article, a subcarrier-multiplexed Optical CDMA system utilizing the direct and AND subtraction method in receiver's detection is explored. The OCDMA part exploits the Recursive Combinatorial (RC) code for its algorithm. The two different designs are then simulated and the performances are compared. Results indicate that the hybrid SCM/OCDMA system using direct detection technique could transmit to a longer distance of up to 50 km with better quality than that with AND subtraction detection for acceptable communication error rate of BER of 10-9.
Hypochondriacal Symptoms Associated With a Less Therapeutic Physician-Patient Relationship

The purpose of this study was to examine the association between hypochondriacal symptoms and the physician-patient relationship. Family medicine patients (n = 310) completed self-report measures of hypochondriacal symptoms, quality of physician-patient relationship, and variables likely to influence that relationship. These variables included physician relationship factors, such as duration of relationship and frequency of visits, as well as patient characteristics, such as neuroticism and positive and negative affectivity. Hypochondriacal symptoms were negatively correlated (r = −.24) with the quality of physician-patient relationship. In addition to hypochondriacal symptoms, the regression model included having a primary physician, length of relationship with that physician, frequency of physician visits, and the level of positive affectivity in the patient. Hypochondriacal symptoms appear to be associated with a less therapeutic physician-patient relationship. Physicians must recognize symptoms of this kind in order to properly address the relationship needs of their patients.
Folate status in various pathophysiological conditions

Folate is the generic term for compounds that have vitamin activity similar to that of pteroylglutamic acid. Folate acts as a coenzyme in several single carbon transfers involved in biosynthesis of purine nucleotides and deoxythymidylic acid essential for DNA and RNA synthesis. In addition, folate provides one‐carbon unit for methylation of a wide variety of biological substances including DNA, proteins, phospholipids, and neurotransmitters, thereby regulating their function. Recent epidemiological‐clinical and experimental studies suggest the association of folate deficiency with the risk of various cancers, birth defects, and cardiovascular diseases. Thus, it is important to consider the conditions that are associated with altered folate status and their consequences. The impairment in folate status has been found in number of pathophysiological conditions like inflammatory bowel disease, cancer, alcoholism, pregnancy, neonatal growth, and during administration of some drugs. The recent advances dealing with mechanistic aspects of impaired folate status in these conditions have been discussed in this review. © 2008 IUBMB IUBMB Life, 60(12): 834–842, 2008