Miracle-dz/UniFAR-FTUs huggingface dataset:
MouseIdentity: Modeling Mouse-Interaction Behavior for a User Verification System

Analysis of mouse-interaction behaviors for identifying individual computer users has experienced growing interest from information security and biometric researchers. This paper presents a simple and efficient user verification system by modeling mouse-interaction behavior, which is accurate and competent for future deployment. For each mouse-operation sample, holistic attributes of mouse trajectories are first analyzed using a power transformation method, to derive a schematic representation of behavior eigenspace. Then, a propagation-based segmentation method is developed to model the detailed dynamic process of mouse movements by performing adaptive behavior segmentation and then characterizing each obtained segment using fine-grained procedural motion metrics. Both schematic and procedural cues from mouse-interaction behaviors may be used independently for verification, being fused at the decision level using combination rules. Analyses are conducted using data from 106 subjects with 21 200 mouse-operation samples. The verification system achieves a 1.96% false-rejection rate and a 1.18% false-acceptance rate with a short verification time (about 6 s) and lightweight system overload. Additional experiments on the effect of sample length and subject pool further examine the applicability of our verification system. We also compare the proposed approach with the state-of-the-art approaches for the data collected. Our findings suggest that mouse-interaction behaviors can enhance traditional authentication systems.
Mobile Ethnography as an Innovative Tool for Customer Experience Research in Tourism – A Case of the Tourism Destination Upper Austria

Service marketing has evolved from focussing on products to services to experiences. Enterprises are nowadays competing for and looking for competitive advantages in experiences rather than customer satisfaction. Even though customer experience management is nothing new, it is a field that has evolved strongly in the last years. Especially in tourism destinations, various local tourism providers struggle with providing high service quality throughout the whole customer journey. Customer experience management has thus become an interesting approach also in tourism research. This paper applies customer experience research by using an innovative research method. Mobile ethnography as a qualitative method transfers the classic ethnographic approach by using mobile devices. Thus, the customer becomes a researcher himself and has the possibility to self-structure his or her data. Especially for tourism destinations, this method allows to “follow” the guest throughout the geographical space that he or she uses and collects data in real-time and in-situ. It therefore overcomes many of the disadvantages of both classic survey studies and ethnography. However briefing of participants and incentivising them turned out to be the main challenges in two projects carried out in the Austrian province of Upper Austria.
Multiple regulatory regions control the complex expression pattern of the mouse Cdx2 homeobox gene.

BACKGROUND & AIMS The Cdx2 homeobox gene exerts multiple functions including trophectoderm specification, antero-posterior patterning, and determination of intestinal identity. The aim of this study was to map genomic regions that regulate the transcription of Cdx2, with a particular interest in the gut. METHODS Genomic fragments covering 13 kilobase (kb) of the mouse Cdx2 locus were analyzed in transgenic mice and in cell assays. RESULTS No fragment was active in the trophectoderm. Fragments containing the first intron and extending up to -5-kb upstream of the transcription start site became active posteriorly at gastrulation and then inactive at midgestation in every tissue including the endoderm. Specific persistence of activity in the intestinal endoderm/epithelium beyond midgestation requires extending the genomic fragment up to -9 kb. We identified a 250-base pair segment around -8.5-kb binding and responding to endodermal factors, with a stimulatory effect exerted synergistically by HNF4alpha, GATA6, Tcf4, and beta-catenin. These factors were able to activate endogenous expression of Cdx2 in nonintestinal Hela cells. CONCLUSIONS Multiple regulatory regions control the complex developmental pattern of Cdx2, including far upstream sequences required for the persistence of gene expression specifically in the gut epithelium throughout life. Cooperation between HNF4alpha, GATA6, beta-catenin, and Tcf4 contributes to the intestine-specific expression of Cdx2.
Aromatase Gene Expression and Regulation in the Female Rat Pituitary

Abstract: Aromatase cytochrome P450, the key enzyme of estrogen biosynthesis from androgens, is encoded by CYP19. Its structure shows some peculiarities: exons II to X encode the protein, while multiple alternative exons I encode unique 5'‐untranslated regions of the aromatase mRNA transcripts. Immunohistochemistry studies in the rat have shown that pituitary aromatase expression is sex‐dependent and varies across the estrous cycle, suggesting that estrogens might be involved in the regulation of aromatase activity and might act locally as a paracrine or autocrine factor in the pituitary. In the present study, we used RT‐PCR to characterize aromatase transcripts and real‐time PCR to quantify the expression of the total aromatase mRNA at the different stages of the estrous cycle and from an ovariectomy and estradiol replacement model. We identified the two previously described aromatase transcripts with a specific 5'untranslated region of the brain 1f and the gonadal PII transcripts. Total aromatase mRNA expression in the pituitary varied significantly during the estrous cycle, with the highest level occurring on the day of metestrus. After ovariectomy, we observed an increase of aromatase mRNA levels, and this effect was completely prevented by estradiol administration. These results suggest that pituitary aromatase mRNA expression is downregulated by estrogens.
The Emerging Role of MORC Family Proteins in Cancer Development and Bone Homeostasis

Microrchidia (MORC or MORC family CW‐type zinc finger protein), a highly conserved nuclear protein superfamily, is an interesting new player in signaling‐dependent chromatin remodeling and epigenetic regulation. MORC family proteins consist of MORC1, MORC2, MORC3, and MORC4 which display common structural determinants such as CW‐type zinc finger and coiled‐coil domains. They also exhibit unique structural motifs and tissue‐specific expression profiles. MORC1 was first discovered as a key regulator for male meiosis and spermatogenesis. Accumulating biochemical and functional analyses unveil MORC proteins as key regulators for cancer development. More recently, using an ENU mutagenesis mouse model, MORC3 was found to play a role in regulating bone and calcium homeostasis. Here we discuss recent research progress on the emerging role of MORC proteins in cancer development and bone metabolism. Unravelling the cellular and molecular mechanisms by which MORC proteins carry out their functions in a tissue specific manner are important subjects for future investigation. J. Cell. Physiol. 232: 928–934, 2017. © 2016 Wiley Periodicals, Inc.
[Relationship between the insulin-like growth factor 1 receptor signaling pathway and the resistance of nasopharyngeal carcinoma to cetuximab].

OBJECTIVE To establish a cetuximab-resistant human nasopharyngeal carcinoma 5-8F/Erbitux cell line and preliminarily study the relationship between the insulin-like growth factor 1 receptor (IGF-1R) signaling pathway and the resistance of nasopharyngeal carcinoma to cetuximab. METHODS A nasopharyngeal cancer cell line, 5-8F, with high epidermal growth factor receptor (EGFR) expression and cetuximab sensitivity, was selected as study object. The cetuximab-resistant 5-8F/Erbitux cell line was induced by stepwise selection after exposure to increasing doses of cetuximab. The IC(50) was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the resistance index (RI) was calculated. The growth curves of 5-8F and 5-8F/Erbitux cells were plotted and the doubling times were calculated by cell counting assay. The cell cycle was detected by flow cytometry. Cross-resistance profiles of 5-8F/Erbitux cells to 5-Fu, Taxol and DDP were tested by MTT assay. Expression levels of P-gP, IGF-1R and P-IGF-1R of 5-8F and 5-8F/Erbitux cells were determined by Western blot analysis and MDR1 gene by real-time fluorescent quantitative PCR. RESULTS A cetuximab-resistant human nasopharyngeal carcinoma cell line 5-8F/Erbitux was successfully established and their resistance index (RI) were 1.2 and 1.1, respectively, at 3 d and 5 d of the cetuximab treatment. The doubling times of 5-8F and 5-8F/Erbitux cells were 26.63 h and 142.30 h, respectively. Flow cytometry demonstrated that 5-8F/Erbitux cells showed an increased population at G(0)/G(1) phase (P < 0.001) and reduced population at S phase (P < 0.001), compared with 5-8F cells. The 5-8F/Erbitux cells showed cross-resistance to 5-Fu (RI = 3.95, P < 0.01) and some resistance to Taxol as well as enhanced sensitivity to DDP (P > 0.05 for all). The 5-8F/Erbitux cells also had increased levels of P-gP, IGF-1R and P-IGF-1R compared with 5-8F cells (P < 0.001 for all). Expression of MDR1 gene was not detected in 5-8F cells and only very weak expression in 5-8F/Erbitux cells. CONCLUSION Cetuximab-resistant 5-8F/Erbitux cells have no common multidrug resistance like that induced by traditional chemotherapeutic drugs. The excessive activation of the IGF-1R signaling pathway is probably one of the mechanisms that caused resistance of 5-8F/Erbitux cells to cetuximab.
Specific and Varied Practice of Motor Skill

To assess the potential effect of specific and varied practice on the development of motor schema, 64 children in two age groups (8 yr. and 12 yr.) were tested on a simple throwing task at the beginning and end of a 12-wk. physical education program. For the throwing test the children were assigned to either a specificity or a schema group (varied practice). The task was performed without visual feedback, but knowledge of results was given after each trial. Prior to both tests at the criterion target, the specific group was given practice throws using the criterion target distance, whereas the schema group practiced on two other targets. No differences were found between the two groups at the beginning of the program but on the posttest those children receiving a variety of practice on the throwing task performed significantly better than the specific-practice group. Thus it was suggested that a varied practice schedule may facilitate the initial formation of motor schema, and this process may be enhanced by participation in a physical education program.
The human brain response to acupuncture on same-meridian acupoints: evidence from an fMRI study.

OBJECTIVES Stimulation of acupuncture points (acupoints) located on meridians has been proven to activate specific areas of the human brain, as evidenced by functional imaging studies. To date, the specificity of acupoints located on different meridians has been investigated with functional magnetic resonance image (fMRI). The present fMRI study explored the effects of stimulating 4 acupoints on 2 different meridians for the purpose of researching the characteristic response of acupoints on the same meridian. MATERIALS AND METHODS The acupoints studied were LR3 (Taichong) and LR6 (Zhongdu), which belong to the Liver meridian of Foot-Jueyin; ST36 (Zusanli) and ST43 (Xiangu), which belong to the Stomach meridian of Foot-Yangming; and 2 nearby sham acupoints. Fifty-three (53) healthy right-handed subjects were divided randomly into 6 groups. Each group only received acupuncture at one acupoint. All the acupoints in this study were stimulated only on the right side of the subject's body. Through analysis of fMRI data from all subjects, we obtained the activation patterns in the central nervous system (CNS) evoked by acupuncture. RESULTS In contrast to the CNS activation by sham acupoint, the results showed that all 4 real acupoints under consideration had the common effect of activating 2 specific areas of the brain: the bilateral primary somatosensory area and the ipsilateral cerebellum. Acupuncture stimulation of both acupoints LR3 and LR6 evoked activation at the ipsilateral superior parietal lobe (BA7). Acupuncture stimulation of both acupoints ST36 and ST43 evoked activation at the ipsilateral middle frontal gyrus (BA10). CONCLUSIONS These results suggest that different acupoints on the same meridian may activate certain similar areas of the brain. In addition, acupoints that are commonly used in clinical practice might modulate a greater extent of cortical areas than uncommonly used acupoints.
Prolactin Supplementation to Culture Medium Improves &bgr;-Cell Survival

Objectives. Recent studies demonstrated that prolactin (PRL) has beneficial effects on &bgr; cells for islet transplantation. We examined the effect of human recombinant PRL (rhPRL) supplementation to the culture media to determine its potential use in the context of clinical islet transplantation. Materials and Methods. Each human islet isolated from 14 deceased multiorgan donors was cultured in Miami modified media-1 supplemented with or without rhPRL (500 &mgr;g/L) for 48 hr. &bgr;-Cell survival and proliferation (BrdU and Ki-67) were determined by laser scanning cytometry. The cytoprotective effects of rhPRL against noxious stimuli were assessed by flow cytometry (tetramethylrhodamine ethyl ester). Cytokine/chemokine and tissue factor productions were measured in vitro, and islet potency was assessed in vivo in diabetic immunodeficient mice. Results. &bgr;-Cell survival during culture was 37% higher in the rhPRL group than in control (P=0.029). rhPRL protected &bgr; cells in vitro from cytokines, Nitric oxide donor, and H2O2. The exposure to rhPRL did not affect human &bgr;-cell proliferation with our protocol. rhPRL treatment did not alter cytokine/chemokine and tissue factor production in vitro or affected human islet functionality in vivo: recipient mice achieved normoglycemia with a comparable tempo, whereas loss of graft function was observed in two of the seven mice in the control group and in none of the rhPRL group (p=n.s.). Conclusion. rhPRL supplementation to islet culture media improved human &bgr;-cell-specific survival without altering islet quality. Addition of rhPRL to cultured islets may grant a more viable &bgr;-cell mass in culture. The development of &bgr;-cell cytoprotective strategies will be of assistance in improving islet transplantation outcomes.
Prevalence and Natural History of Celiac Disease in a Cohort of At-risk Children

Objectives: To assess the prevalence and clinical presentation of celiac disease (CD) in a cohort of children with HLA-DQ2 positive and evaluate the risk factors in the development of CD. Methods: Between July 2004 and July 2005, parents of all healthy full-term newborns in our hospital were invited to participate. HLA-DQ2 was tested in blood sample of the umbilical cord. A point of contact serological test was performed on children between 2 and 3 years of age. Positive results were confirmed by serum anti-transglutaminase 2 and endomysial antibodies. Children with high autoantibody titers underwent an intestinal biopsy. Children of the cohort diagnosed with CD before the screening study were included. Sex, mode of delivery, breast-feeding duration, and age of gluten introduction were studied. Results: Of 1291 children, 362 were HLA-DQ2 positive and 262 participated in the study. CD was diagnosed in 4.1% (95% confidence interval (CI) 1.9–6.3). In the whole cohort, 60% had gastrointestinal symptoms, 7% poor weight gain, and 33% were asymptomatic. Five children with potential CD and 6 with CD autoimmunity became negative (42.3%) and are still negative after 5 to 7 years. Female sex was at-risk factor odds ratio 5.7 (95% CI 1.5–20.9), whereas breast-feeding during gluten introduction had a protective effect odds ratio 0.1 (95% CI 0.01–0.8). Conclusions: Prevalence of CD in this cohort was 4%, half of whom had digestive symptoms. Because a high proportion of children showed a spontaneous disappearance of antibodies, prevalence studies of CD in young children should be based on intestinal damage so as not to overestimate results.
Computing structural controllability of linearly-coupled complex networks

Structural controllability, as a generic structure-based property in determining the ability of a complex network to reach the desired configuration, is addressed in this work. Using a robust measure derived from robust control theory, this paper deals with structural controllability of a type of weighted network of networks (NetoNets) involving linear couplings between its corresponding networks and clusters. Unlike the structural controllability degrees rooted in graph theory, this paper takes the advantage of uncertain systems to define the notion of structural controllability in a straightforward and less computationally complex way. Moreover, the spectrum of required energy is discussed. Eventually, the results for the proposed measure of structural controllability of scale-free networks are given to justify the proposed measure of an efficient and effective guarantee for fully controllability of the NetoNets in exposure to cluster and network-dependency connections. The proposed measure is an optimal solution according to structural energy-related control of the NetoNet where the upper bound of the required energy is illustrated an efficient measure for structural controllability of the class of NetoNet. Arbitrarily connectivity of low connected vertices to their higher connected counterparts in clusters results in effective controllability. In the same direction, as seminal works in structural controllability of complex networks to avoid the highly-connected nodes, the larger the cluster/network connectivity degree is, the less fully controllability of NetoNet is guaranteed.
The serine protease inhibitor neuroserpin is required for normal synaptic plasticity and regulates learning and social behavior

The serine protease inhibitor neuroserpin regulates the activity of tissue-type plasminogen activator (tPA) in the nervous system. Neuroserpin expression is particularly prominent at late stages of neuronal development in most regions of the central nervous system (CNS), whereas it is restricted to regions related to learning and memory in the adult brain. The physiological expression pattern of neuroserpin, its high degree of colocalization with tPA within the CNS, together with its dysregulation in neuropsychiatric disorders, suggest a role in formation and refinement of synapses. In fact, studies in cell culture and mice point to a role for neuroserpin in dendritic branching, spine morphology, and modulation of behavior. In this study, we investigated the physiological role of neuroserpin in the regulation of synaptic density, synaptic plasticity, and behavior in neuroserpin-deficient mice. In the absence of neuroserpin, mice show a significant decrease in spine-synapse density in the CA1 region of the hippocampus, while expression of the key postsynaptic scaffold protein PSD-95 is increased in this region. Neuroserpin-deficient mice show decreased synaptic potentiation, as indicated by reduced long-term potentiation (LTP), whereas presynaptic paired-pulse facilitation (PPF) is unaffected. Consistent with altered synaptic plasticity, neuroserpin-deficient mice exhibit cognitive and sociability deficits in behavioral assays. However, although synaptic dysfunction is implicated in neuropsychiatric disorders, we do not detect alterations in expression of neuroserpin in fusiform gyrus of autism patients or in dorsolateral prefrontal cortex of schizophrenia patients. Our results identify neuroserpin as a modulator of synaptic plasticity, and point to a role for neuroserpin in learning and memory.
Exosome proteomics reveals transcriptional regulator proteins with potential to mediate downstream pathways

Exosomes are virus‐sized, membrane‐enclosed vesicles with origins in the cellular endosomal system, but are released extracellularly. As a population, these tiny vesicles carry relatively enormous amounts of information in their protein, lipid and nucleic acid content, and the vesicles can have profound impacts on recipient cells. This review employs publically‐available data combined with gene ontology applications to propose a novel concept, that exosomes transport transcriptional and translational machinery that may have direct impacts on gene expression in recipient cells. Here, we examine the previously published proteomic contents of medulloblastoma‐derived exosomes, focusing on transcriptional regulators; we found that there are numerous proteins that may have potential roles in transcriptional and translational regulation with putative influence on downstream, cancer‐related pathways. We expanded this search to all of the proteins in the Vesiclepedia database; using gene ontology approaches, we see that these regulatory factors are implicated in many of the processes involved in cancer initiation and progression. This information suggests that some of the effects of exosomes on recipient cells may be due to the delivery of protein factors that can directly and fundamentally change the transcriptional landscape of the cells. Within a tumor environment, this has potential to tilt the advantage towards the cancer.
Complete and Draft Genome Sequences of Six Members of the Aquificales

ABSTRACT The Aquificales are widespread in marine and terrestrial hydrothermal environments. Here, we report the complete and draft genome sequences of six new members of the Aquificales: two marine species, Persephonella marina strain EX-H1 and Hydrogenivirga strain 128-5-R1 (from the East Pacific Rise, 9°50.3′N, 104°17.5′W, and the Eastern Lau Spreading Center, 176°11.5′W, 20°45.8′S, respectively), and four terrestrial isolates, Sulfurihydrogenibium azorense strain Az-Fu1, Sulfurihydrogenibium yellowstonense strain SS-5, and Sulfurihydrogenibium strain Y03AOP1 (from Furnas, Azores, Portugal, and Calcite Springs and Obsidian Pool in Yellowstone National Park, United States, respectively), and the only thermoacidophilic isolate, Hydrogenobaculum strain Y04AAS1 (from a stream adjacent to Obsidian Pool). Significant differences among the different species exist that include nitrogen metabolism, hydrogen utilization, chemotaxis, and signal transduction, providing insights into their ecological niche adaptations.