Miracle-dz/UniFAR-FTUs huggingface dataset:
A Case of Extra-Skeletal Intracranial Chondromass: Ultrastructure and Immunohistochemical Features

The discrete microvasculature environment of the perivascular plays a dominant role in regulating stem cell activity and its proliferation behavior. Mesenchymal stem cells (MSC) reside in perivascular compartment, hence have the potential to contribute to the pathogenesis of extra-skeletal intracranial chondroma (ESICC). In this case report, for the first time, the immunohistochemical (IHC) markers and transmission electron microscpoe (TEM) features of perivascular are presented in ESICC. Microscopic examination revealed a well-differentiated chondroma with foci of ossification and islands of vacuolated-cells encircling thin-walled capillaries. TEM showed transitional morphological features suggestive of transformation to chondrocytes, with vacuolated cells. A subpopulation of spindle vacuolated-cells displayed reaction for S-100, ferritin, trypsin-anti-chymotrypsin and Vimentin. As there was no evidence of transitional forms to arachnoid cells, it can be concluded that the origin of the recognized ICC is a perivascular multipotential mesenchymal cell, MSC.
Interactions des microARN de la famille miR-34/449 avec les voies de signalisation intracellulaire : rôle dans la différenciation des cellules multiciliées chez les vertébrés

Les cellules multiciliees (MCC) possedent a leur surface apicale des centaines de cils mobiles generant un flux directionnel liquidien necessaire par exemple pour le nettoyage des voies respiratoires. La fabrication de ces cils (multiciliogenese) requiert une sequence d’evenements cellulaires dont un arret du cycle cellulaire, une reorganisation du reseau apical d’actine, une multiplication massive des centrioles suivie de leur migration au pole apicale et de leur maturation en corps basal, a partir desquels les cils s’allongent.Mon laboratoire d’accueil a mis en evidence le role conserve de la famille de microARN miR-34/449 dans le controle de la multiciliogenese en inhibant la voie de signalisation Notch ainsi qu’en induisant un arret du cycle. Au cours de ma these, j’ai mis en evidence un nouveau niveau de regulation de ces microARN par lequel ils controlent la reorganisation apicale du cytosquelette d’actine, en modulant l’expression et l’activite de certaines petites GTPases. Par ailleurs, j’ai identifie et caracterise des sequences variantes des miR-34/449 canoniques, appelees isomiR. Tandis que ces isomiR partagent des fonctions semblables a celles de leurs homologues canoniques, ils apportent egalement une complementarite d’action en modulant des transcrits cibles specifiques. Enfin, le dernier axe de mon travail a permis d’identifier le role de la voie de signalisation BMP dans la multiciliogenese ainsi que d’elucider certains des mecanismes moleculaires par lesquels elle controle ce phenomene. L’ensemble de nos decouvertes offre une opportunite inedite pour developper des strategies therapeutiques dans le traitement de maladies associees a des desordres ciliaires
DETECTION OF P16 INK4A PROMOTER METHYLATION IN THE BLOOD SAMPLE COMPARED WITH CLINICO-PATHOLOGICAL FINDINGS OF CERVICAL CANCER PATIENTS: A NORTH INDIAN STUDY

Epidemiologic and clinical data show that the development of cervical cancer is a multifactorial process in which infection with human papillomavirus (HPV) takes a central place along with other risk factors such as smoking, immunosuppression, immunodeficiency, diet, parity, age at first full-term pregnancy and family history. Detection of p16 Methylation in Blood Samples Compared with Clinicopathological findings analyzed in the north India population among cervical cancer patients. Methylation status of p16 promoter was analysed in cervical cancer and controls subjects by PCR using specific primer for p16 after methylation-insensitive restriction enzyme digestion of genomic DNA. Methylated status of p16 gene promoter was analysed in cervical cancer cases and controls using RE digestion followed by PCR. The methylation-insensitive restriction enzymes cut regions containing unmethylated CpG sequences at the promoter region. Methylation pattern analysis of cervical cancer out of 64 cases 51(79.7%) of them contained methylated CpG island at promoter region compare to controls containing only 13(40.6%) out of 32 subjects methylated CpG. It was observe that methylated p16 promoter subjects have higher risk of cervical cancer in comparison with unmethylated p16 promoter subjects. This result supports that p16 methylation in blood could be a novel marker for the malignancy of cervical cancer.
THE EFFECT OF AGE OF BIRD ON SHELL QUALITY AND COMPONENT YIELD OF EGGS

The effect or age of birds on shell quality and yield components of egg was studied. The birds' age were 25, 30, 58 or. 78 weeks. The eggs' weight increased by 9.92%, 26.43% and 31.46% for birds at 30, 58 and 78 weeks, respectively. While the shell weight was found to increase by 15.10, 34.55 and 30.44% for birds at 30, 58 and 78 weeks, respectively. The yolk and albumen weights were found to increase by 4.05%, 11.39%, 44.72%, 18.06% and 44.72%, 26.41%. for hens . at 30, 58 and 78 week.s old respectively. The yolk: albumen ratio was highest (0.478 ±0.12) with eggs laid by 58 weeks old birds. Albumen pH decreased with increase in the age of birds. Keywords: Shell quality, component yield of egg
Estimation of digestible tryptophan:lysine ratios for maximum performance, egg quality and welfare of white-egg-laying hens by fitting the different non-linear models

ABSTRACT A total of 360 Hy-Line W-36 hens at 30 wk of age were assigned to 6 dietary treatments that consisted of 6 different (11, 15, 19, 23, 27 and 31) digestible Trp:Lys ratios. Hens were fed the experimental diets from 30 to 36 wk of age. Productive traits were recorded every wk and egg quality was measured at 36 wk of age. Total IgM, IgG, anti-sheep red blood cell antibody titres, plasma corticosterone, serotonine and glucose concentrations and heterophil to lymphocyte ratio (H:L) were determined at wk 6 of the experiment. Results showed a significant improvement (P < .0001) in egg production, egg mass, feed intake, feed conversion ratio and body weight by increasing the standardized ileal digestible (SID) Trp:Lys ratio from 11 to 19. Relative weights of shell (P < .05) and shell strength (P < .06) were affected by dietary treatments. Hens fed graded levels of SID Trp responded by decreasing the H:L ratio in a linear or curvilinear manner (P < .05). The study reported here indicated that the ideal SID Trp:Lys ratio for laying hens ranged from17.5 to 29 based on the response criteria and regression models.
Concepts for the Production of Viruses and Viral Vectors in Cell Cultures

The industrial-scale manufacturing of viruses or virus-like particles in cell culture is necessary for gene therapy and the treatment of cancer with oncolytic viruses. Complex multistep processes are required in both cases, but the low virus titers in batch cultures and the temperature sensitivity of the virus particles limit the production scale. To meet commercial and regulatory requirements, each process must be scalable and reproduc-ible and must yield high virus titers. These requirements are met by establishing a cell culture process that matches the properties of the virus/host-cell system and by using serum-free cell culture medium. This chapter focuses on two case studies to consider the different aspects of process design, such as the reactor configuration and operational mode: the continuous production of retroviral pseudotype vectors in a retroviral packaging cell line and the production of oncolytic measles virus vectors for cancer therapy.
Sexually inexperienced anestrous goats are able to exhibit sexual behaviours exposed to sexually active bucks.

The aim of this study was to determine whether sexually inexperienced females could display proceptivity and receptivity behaviours as the experienced in the first exposure to males. Three groups of females (n=9 each) were used: i) sexually inexperienced, ii) with complete sexual experience, and iii) with limited sexual experience. Three male goats were subjected to photoperiodic treatment for 2.5 months of long days to stimulate their sexual activity during the natural sexual rest (March-April). During anestrous season, females were exposed to photo-stimulated males. Sexual behaviours were recorded during the first three days post-introduction of the males into female groups, in two daily periods of 20 min each. Sexually inexperienced females and those with complete sexual experience showed higher tail wagging than those with limited sexual experience (P 0.05). We concluded that sexually inexperienced anestrous females display proceptivity and receptivity behaviours as those sexually experienced exposed to photo-stimulated males.
NOVEL IRSP53 (INSULIN RECEPTOR SUBSTRATE PROTEIN OF 53 KDA) FUNCTIONS IN ACTIN CAPPING, FILOPODIA FORMATION AND POLARITY ESTABLISHMENT

12 Introduction 14 The actin cytoskeleton 15 The actin monomer and the stages of actin polymerization 17 Lamellipodia and filopodia 20 Note on GTPases 22 Actin-­‐binding proteins 23
17-beta estradiol alters the innate immune response to Neisseria gonorrhoeae

Data compiled by the Centers for Disease Control demonstrates that African American women, in particular young people between the ages of 14-25, have an increased incidence of infection with the sexually transmitted pathogen Neisseria gonorrhoeae. Estradiol is a key regulatory hormone in female reproductive function. It has been studied extensively in the cardiovascular field, and has been linked to breast and endometrial cancers in women. However, its impact on infectious diseases is largely unknown. Given what is known about the effect of estradiol on immunologic and inflammatory disorders in women, I hypothesize that estradiol alters the infectivity of Neisseria gonorrhoeae in the female reproductive tract by altering the host inflammatory immune response. This may explain a risk factor for increased rates of infection in some populations. I sought to develop a relevant in vitro model. After screening a number of candidate cell lines, I selected the human endometrial adenocarcinoma cell line, Ishikawa. These cells express specific estrogen receptors and respond to exogenous estrogen stimulation. Estrogen treatment of Ishikawa cells did not have an impact on the invasion of N. gonorrhoeae, nor did it impact bacterial growth. However, gonococcal induced chemokine secretion was reduced by estrogen, as measured by interleukin-8 vii secretion. I conclude that estrogen blunts the inflammatory response to Neisseria gonorrhoeae without altering bacterial infectivity.
Different brain networks underlying intelligence in Autism Spectrum Disorders and Typically Developing Children

There has been sustained clinical and cognitive neuroscience research interest in the neural basis of intelligence. The characterisation of brain structure and function underlying cognitive performance is necessary to understand the neurodevelopment of intelligence across the lifespan, and how associated neural correlates could be perturbed in atypical populations. As most work in this area has focused on neurotypical adults, the nature of functional brain connectivity underlying intelligence in paediatric cohorts with or without abnormal neurodevelopment requires further investigation. We use network-based statistics (NBS) to examine the association between resting-state functional Magnetic Resonance Imaging (fMRI) connectivity and fluid intelligence ability in male children with Autism Spectrum Disorders (ASD; M=10.45, SD=1.58 years, n=26) and in matched controls (M=10.38, SD=0.96 years). Compared to typically developing controls strictly matched on age, sex and fluid intelligence scores, boys with ASD displayed a subnetwork (network size=24, p=.0373, FWE-corrected) of significantly increased associations between functional connectivity and fluid intelligence performance. Between-group differences remained significant at a higher edge threshold of t=4 (size=6, p=.0425, FWE-corrected). Results were validated in independent-site replication analyses representing a similar male cohort with ASD (network size=14, p=.0396, FWE-corrected). Regions implicated in atypical ASD fluid intelligence connectivity were the angular gyrus, posterior middle temporal gyrus, occipital and temporo-occipital regions. Across all sites, within-group analyses failed to identify functional connectivity subnetworks associated with fluid or general intelligence performance in matched typically developing males. Findings suggest a prematurely accelerated but aberrant development of fluid intelligence neural correlates in young ASD males, possibly as a compensation mechanism that supports equivalent task performance to controls. The absence of whole-brain network correlates of general and fluid intelligence in young neurotypical males may represent the shift from local to global integration in the development of cognitive ability.
Smart Toll and Penalty Collection System

Developing countries like India need a significant improvement in infrastructure such as Roads or Highways. Construction of these highways is a costly affair, which can’t be invested by the government alone. Normally Public private partnerships are made to construct such a huge projects. The money spent on these projects can be regained by collecting toll from the passengers who use the roads. The toll collection system, especially in India faces some problems such as long queue lines, escaping from toll plazas etc. These systems can service only 300 vehicles per hour, and if more than that number of vehicles arrives at that plaza, server traffic jams may occur[7]. With the increase in the number of vehicles on road, there has been a marked increase in the number of crimes involving vehicle theft. In spite of several stringent laws being in place and security measures taken by car manufacturers, thieves still find a way to remain one step ahead and vehicle theft is still among one of the most reported crimes worldwide. Due to the expensive nature of motor vehicles, there is ample incentive for petty thieves to attempt thefts. To solve both problems we propose QR Code base toll collection system. QR Code is generated at the time of registration of vehicle in our proposed system. On toll collection booth we collect toll as well as identify vehicle is stolen or not. Second module is to give easy work to traffic police to collect penalty through smart application. Index Terms Dynamic pricing; dynamic shuttle; dynamic traffics; Mobility on Demand (MoD); transitive control; urban mobility, QR Code, Penalty collection, toll plaza, etc.
Genetic Variability, Heritability and Genetic Advance for Yield Traits in Tomato (Solanum lycopersicum L.)

among vegetable crops and 7 th in the list of important crop species worldwide. In India, it is next only to potato and onion among vegetables in terms of production. Since its introduction in 16 th century, it has spread to almost all the parts of the world and is being grown commercially in 159 countries (Saker et al., 2011). The crop has become popular among farmers because of its short duration, high yield potential, high profitability and economic viability. On account of its high nutritive (Ram, 2005) and protective values (Chauhan et al., 2014), it is one of the most consumed vegetables in different forms. It is highly valued in processing industries for its peculiar sensorial properties.
3D models related to the publication: Protocetid (Cetacea, Artiodactyla) bullae and petrosals from the Middle Eocene locality of Kpogamé;, Togo: new insights into the early history of cetacean hearing

This contribution contains the 3D models described and figured in the following publication: Mourlam, M. J., Orliac, M. J. (2017), Protocetid (Cetacea, Artiodactyla) bullae and petrosals from the Middle Eocene locality of Kpogamé, Togo: new insights into the early history of cetacean hearing. Journal of Systematic Palaeontology https://doi.org/10.1080/14772019.2017. 1328378
Effect of nitrogen supply before bud break on early development of the young hybrid poplar

Nitrogen status widely impacts tree physiological process. However, most research concentrated on endogenous carbon and nitrogen remobilization in spring neglected the hypothesis of significant effect of nitrogen uptake before bud break on nitrogen and carbohydrate reserve used, and the quality of new growth. This study undertook experimental-based research on young poplar (Populus tremula × Populus alba, clone INRA 717-1B4) with different structures. Three series of experiments were conducted in a coordinated manner : the one year old scion (i), the young stump (ii), and the system then reiterated associated the “stump”, and the “scion” (iii). The experiments were to study plants in a controlled environment with soilless culture and three terms of nitrogen supply: without nitrogen supply (i), with a limited supply prior to bud break (ii), and with continuous nitrogen supply (iii). Results show that poplar can uptake nitrate before bud break and it found to have significant effect by induce a strong root pressure which in turn increased water content of all tissues. Accordingly, the effect of nitrogen uptake before bud break on bud break time depended on plant architecture, bud structure, and temperature during winter to break down bud dormancy. In addition, nitrogen uptake before bud break had significantly effect on the growth and development of new shoots after bud break by increase leaf area and dry weight of new shoots. It also influenced on the quantity of nitrogen and non-structural carbon reserves in all tissues especially increase nitrogen contents in roots and new shoots. Therefore, results indicate nitrogen supply before bud beak plays a significant role on plant physiology and quality of the re-growth.
Biochemical properties of a serine protease from Aspergillus flavus and application in dehairing

Abstract The proteases are enzymes produced by several filamentous fungi with important biotechnological applications. In this work, a protease from Aspergillus flavus was characterized. The culture filtrate of A. flavus was purified to homogeneity by Sephacryl S-200 column chromatography followed by CM–cellulose. The molecular weight of the purified enzyme was estimated to be approximately 32 kDa by SDS–PAGE. The enzyme hydrolysed BTpNA (N-α-benzoyl-dl-tyrosyl-p-nitroanilide), azo-casein and casein as substrates. Optimal temperature and pH were 55 °C and 6.5, respectively. The enzyme was stimulated by Mg2+, Ca2+, Zn2+ and inhibited by Hg2+ and Ag2+ and Cu2+. The protease showed increased activity with detergents, such as Tween 80 and Triton X, and was stable to the reducing agents, such as β-mercaptoethanol. The protease activity was strongly inhibited in the presence of phenylmethylsulfonyl fluoride, indicating it is a serine protease. The enzyme entrapped in calcium alginate beads retained its activity for longer time and could be reused up to 10 times. The thermostability was increased after the immobilization and the enzyme retained 100% of activity at 45 °C after 60 min of incubation, and 90% of residual activity at 50 °C after 30 min. In contrast, the free enzyme only retained 10% of its residual activity after 60 min at 50 °C. The enzymatic preparation was demonstrated to be efficient in the capability of dehairing without destruction of the hide. The remarkable properties such as temperature, pH and immobilization stability found with this enzyme assure that it could be a potential candidate for industrial applications.
The effect of variation of dose, time toward cauli flower (Brassica oleracea var BotrytisL.) On hepatic cytochrome P-450 level rats given theophylline

Cytochrome P-450 as a major component enzyme system in drug metabolism. Activity enzyme of cytochrome P-450 was influenced by internal and external factors. Vegetables of Brassicaceae such as cauli flower were often consumed in the long term, its has inductor activity of oxidation enzyme systems and conjugation reactions. Theophylline as a bronchodilator drugs have the unknown effects on hepatic microsomal enzyme, such as cytochrome P-450 and have not been many studies that tried to prove it. The purpose of this study was to prove the effects of Cauliflower and indol on level cytochrome P-450 enzyme. 90 rats were divided into 3 groups. Group I, were given Theophylline 20 mg/kg BW. In group II 30 rats were treated with indole 1,2; 2,4; 3,6 mg/kg BW, and group III 30 rats were treated with cauliflower extract respectively doses 100, 200, 300 g/kg BW. Each dose was given on 10 rats, each group were divided 2 sub-groups were treated for 5, 10 days. On the last day of treatment were given Theophylline 20 mg/kg BW. Cytochrome P-450 enzyme levels were determined by the method of Omura and Sato(Snell, and Mullock,1987). An induction cauliflower and indole did not increased levels of hepatic cytochrome P-450. The long treatment and the increased of administered dose did not enhanced the levels of hepatic cytochrome P-450 enzyme. cauliflower and indole contained in vegetables when consumed together with theophylline drug, would not affect the metabolism of theophylline Key words: Cauli flower, cytochrome P-450, theophylline, indole, spectrofotometric.
Effect of humic acid on Meloidogyne incognita (Kofoid & White) Chitwood infecting banana (Musa spp.)

ABSTRACT Humic acid, a natural by-product from lignite coals, was evaluated for its potential to control root-knot nematode, Meloidogyne incogntia infecting banana. Hatching of M. incognita eggs was inhibited 50%–100% following incubation in 0.08%-2.0% humic acid in vitro. Exposure of juveniles (J2) of M. incognita to different humic acid concentrations significantly affected the mobility of J2 in vitro. The percent immobility of J2 increased with concentrations of humic acid up to 0.08%. In pot experiments, soil treatment with humic acid reduced the root galling in banana plantlets. All the humic acid tested concentrations (0.04%, 0.08%, 0.2% and 0.4%) significantly reduced the nematode soil density by 53.5%–56.7%, root infection by 61.9%–63.8%, egg population by 61.9%–63.8% and reproduction rate by 55.7%–56.6%. A significant improvement of growth of banana plantlets in terms of number of leaves, pseudostem height, girth, weight, root length and weight was noticed in humic acid treated pots. Humic acid did not suppress growth of the biocontrol agents Pseudomonas fluorescens and Trichoderma viride in vitro. Results suggest that soil treatment with humic acid at 0.04% not only offers significant nematode control but also improves growth of banana.
Are nectar guide colour changes a reliable signal to pollinators that enhances reproductive success?

Background: Ageing and post-pollination changes in floral colour occur widely in flowering plants, but it remains an open question as to whether or not colour changes in nectar guides are associated with the quantity of floral rewards that ultimately influence pollinator visitations and reproductive success. Aims: To examine whether nectar guide changes should be considered as a reliable signal to pollinators and to assess the effects of nectar guide changes on reproductive success. Methods: We studied the process and adaptive value of colour changes in the nectar guides of Arnebia szechenyi whose flowers typically display conspicuous nectar guides at the onset of anthesis, after which they begin to fade, and disappear completely on the second day. Results: Changes in nectar guide colour in A. szechenyi were intrinsic and age-dependent, although pollination somewhat accelerated the change. By the time that the nectar guides disappeared completely, floral rewards were reduced almost to zero. Artificial removal of nectar guides decreased both fruit set and pollen export. Flowers without nectar guides do not appear to increase the overall attractiveness of the plants. Conclusions: Nectar guides and their changes represent reliable signals to pollinators and enhance both male and female reproductive success.