Miracle-dz/UniFAR-FTUs huggingface dataset:
MYC2 regulates ARR16, a component of cytokinin signaling pathways, in Arabidopsis seedling development

Abstract MYC2 is a basic helix‐loop‐helix transcription factor that acts as a repressor of blue light‐mediated photomorphogenic growth; however, it promotes lateral root formation. MYC2 also regulates different phytohormone‐signaling pathways in crucial manner. Arabidopsis response regulator 16 (ARR16) is a negative regulator of cytokinin signaling pathways. Here, we show that MYC2 directly binds to the E‐box of ARR16 minimal promoter and negatively regulates its expression in a cytokinin‐dependent manner. While ARR16 and MYC2 influence jasmonic acid and cytokinin signaling, the expression of ARR16 is regulated by cry1, GBF1, and HYH, the components of light signaling pathways. The transgenic studies show that the expression of ARR16 is regulated by MYC2 at various stages of development. The mutational studies reveal that ARR16 positively regulates the hypocotyl growth in blue light, and phenotypic analysis of atmyc2 arr16 double mutant further reveals that arr16 can suppress the short hypocotyl phenotype of atmyc2. Altogether, this work highlights MYC2‐mediated transcriptional repression of ARR16 in Arabidopsis seedling development.
A novel case of autogamy and cleistogamy in Dendrobium wangliangii: A rare orchid distributed in the dry‐hot valley

Abstract Dendrobium wangliangii is an epiphytic orchid distributed in the Jinshajiang dry‐hot valley in Luquan County, Yunnan Province, China. Most Dendrobium spp. typically have a low fruit set, but this orchid shows a higher fruit set under natural conditions despite the lack of effective pollinators. The pollination biology of the critically endangered D. wangliangii was investigated in this study. A fruit set rate of 33.33 ± 4.71% was observed after bagging treatment in 2017 and a high fruit set rate (65.72 ± 4.44% in 2011; 50.79 ± 5.44% in 2017) was observed under natural conditions, indicating that D. wangliangii is characterized by spontaneous self‐pollination. The anther cap blocked the growing pollinium; thus, the pollinium slid down and reached the stigmatic cavity, leading to autogamous self‐pollination. Specifically, 51.50% of 162 unopened flowers (total 257 flowers) of this Dendrobium species under extreme water‐deficit conditions developed into fruits, suggesting the presence of cleistogamy in D. wangliangii. Here, cleistogamy may represent the primary mode of pollination for this orchid. Spontaneous self‐pollination and specific cleistogamous autogamy could represent major adaptions to the drought and pollinator‐scarce habitat in the Jinshajiang dry‐hot valley.
Practical guide to choosing dabigatran 150 mg twice daily or apixaban 5 mg twice daily for patients with atrial fibrillation

Based on previous reports, we propose a practical guide to choose dabigatran 150 mg twice daily or apixaban 5 mg twice daily for patients with atrial fibrillation. We recommend the use of dabigatran 150 mg twice daily for patients with atrial fibrillation who have a high risk of embolism (e.g., ischemic stroke on other oral anticoagulants, presence of left atrial appendage thrombus) and a low risk of bleeding. However, the prevalence of such patients with atrial fibrillation is considered low because patients with atrial fibrillation with a high risk of embolism usually have a high risk of bleeding. In most other patients with atrial fibrillation, the use of apixaban 5 mg twice daily should be considered.
Soil respiration of four forests along elevation gradient in northern subtropical China

Abstract Background and aims Soil respiration is the second‐largest terrestrial carbon (C) flux, and soil temperature and soil moisture are the main drivers of temporal variation in soil respiration and its components. Here, we quantified the contribution of soil temperature, soil moisture, and their intersection on the variation in soil respiration and its components of the evergreen broad‐leaved forests (EBF), mixed evergreen and deciduous broad‐leaved forests (MF), deciduous broad‐leaved forests (DBF), and subalpine coniferous forests (CF) along an elevation gradient. Methods We measured soil respiration of four types of forests along the elevation gradient in Shennongjia, Hubei China based on the trenching experiments. We parameterized the relationships between soil respiration and soil temperature, soil moisture, and quantified the intersection of temperature and moisture on soil respiration and its components. Results Total soil respiration (R S), heterotrophic respiration (R H), and autotrophic respiration (R A) were significantly correlated with soil temperature in all four forests. The Q 10 value of soil respiration significantly differed among the four types of forest, and the Q 10 was 3.06 for EBF, 3.75 for MF, 4.05 for DBF, and 4.49 for CF, respectively. The soil temperature explained 62%–81% of the variation in respiration, while soil temperature and soil moisture together explained 91%–97% of soil respiration variation for the four types of forests. The variation from the intersection of soil temperature and moisture were 12.1%–25.0% in RS, 1.0%–7.0% in R H, and 17.1%–19.6% in R A, respectively. Conclusions Our results show that the temperature sensitivity (Q 10) of soil respiration increased with elevation. The intersection between soil temperature and soil moisture had strong effects on soil respiration, especially in R H. We demonstrated that the intersection effects between soil temperature and soil moisture on soil respiration were essential to understand the response of soil respiration and its components to climate change.
Early Prediction of Treatment Response of Neuroendocrine Hepatic Metastases after Peptide Receptor Radionuclide Therapy with 90Y-DOTATOC Using Diffusion Weighted and Dynamic Contrast-Enhanced MRI

The purpose of this study was to determine if parameters derived from diffusion-weighted (DW-) and dynamic contrast-enhanced (DCE-) magnetic resonance imaging (MRI) can help to assess early response to peptide receptor radionuclide therapy (PRRT) with 90Y-DOTATOC in neuroendocrine hepatic metastases (NET-HM). Twenty patients (10 male; 10 female; mean age: 59.2 years) with NET-HM were prospectively enrolled in this single-center imaging study. DW-MRI and DCE-MRI studies were performed just before and 48 hours after therapy with 90Y-DOTATOC. Abdominal SPECT/CT was performed 24 hours after therapy. This MRI imaging and therapy session was repeated after a mean interval of 10 weeks. Up to four lesions per patient were evaluated. Response to therapy was evaluated using metastasis sizes at the first and second therapy session as standard for comparison (regressive, stable, and progressive). DW-MRI analysis included the apparent diffusion coefficient (ADC) and parameters related to intravoxel incoherent motion (IVIM), namely, diffusion (D), perfusion fraction (f) and pseudo-diffusion (D∗). DCE-MRI analysis comprised Ktrans, ve and kep. For statistical analysis of group differences, one-way analysis of variance (ANOVA) and appropriate post hoc testing was performed. A total of 51 lesions were evaluated. Seven of 51 lesions (14%) showed size progression, 18/51 (35%) regression, and 26/51 (51%) remained stable. The lesion-to-spleen uptake ratio in SPECT showed a decrease between the two treatment sessions that was significantly stronger in regressive lesions compared with stable (p = 0.013) and progressive lesions (p = 0.021). ANOVA showed significant differences in mean ADC after 48 h (p = 0.026), with higher ADC values for regressive lesions. Regarding IVIM, highest values for D at baseline were seen in regressive lesions (p = 0.023). In DCE-MRI, a statistically significant increase in ve after 10 weeks (p = 0.046) was found in regressive lesions. No differences were observed for the transfer constants Ktrans and kep. Diffusion restriction quantified as ADC was able to differentiate regressive from progressive NET-HMs as early as 48 hours after PRRT. DW-MRI therefore may complement scintigraphy/SPECT for early assessment of response to PRRT. Assessment of perfusion parameters using IVIM and DCE-MRI did not show an additional benefit.
Differential prolactin levels among male and female patients with thyroid related complains in the Hail regions of Saudi Arabia

Thyroid diseases are caused by autoimmunity due to hormone imbalance both in male and female patients. Therefore, it is of acute importance to measure, analyze and compare thyroid hormone levels among populations with thyroid-related complications. Hence, we examined 202 male and female thyroid patients in the Hail regions of Saudi Arabia and estimated their hormone levels. Blood samples were collected from patients and processed for the hormonal profiling such as prolactin, luteinizing hormone (LH), FSH, free T3 (FT3), free T4 (FT4) and TSH3. Further, measurement of thyroid gland size in the Hail population was also completed. Results of our study showed a significant difference in the level of prolactin between male and female patients. Other hormones are namely luteinizing hormone (LH), FSH, free T3 (FT3), free T4 (FT4), TSH3 did not show any significant difference between male and female patients with thyroid disorder. Thus, the levels of the majority of hormones, namely luteinizing hormone (LH), FSH, free T3 (FT3), free T4 (FT4), and TSH3, except prolactin, did not differ significantly between male and female thyroid patients. Validation of the observation using large scale population size is warranted in future investigations.
ATTITUDES, PRACTICE AND ADDICTION TOWARDS WATER-PIPE / SHISHA SMOKING IN LAHORE CITY

This study was aimed at assessing the various factors associated with shisha smoking such as attitudes, practice and addiction among the adult population aged 15 - 44 years in Lahore city. A cross-sectional survey was held in Shisha bars, tobacco shops and Shisha cafes along with some major undergraduate colleges and Universities of Lahore in summer of 2013. All adults aged between 15 and 44 years of age who consumed Shisha were included in the sample. All other age groups and people not smoking were excluded. The data for this study were collected with the participants using an approved self- administered questionnaire. Data analysis was done using the IBM SPSS version 20. The final sample size was 300 (male 194 and female 106). The largest group was of young adults aged 21-25 years. Out of all the participants, 65% of the sample was smoking shisha for over a year. It was noteworthy that encouragement for shisha smoking was brought on more by friends (61%) as compared to colleagues (9%) and the money spent on such activities was utilized from their own pocket money (70.3%). It was also observed that 42.3% of the sample recognized water-pipe/shisha as being a stress reliever and 68.7% admitted it to be a status symbol. Hence, 77.7% of the sample smoked shisha in the company of friends with 11.3% claiming to be completely addicted to it. In addition, 72.3% of the participants of this study refused to quit water-pipe/shisha smoking. Significance was found in Pearson’s correlation between gender and duration, expenditure and frequency of consumption, addiction and duration, status symbol and stress reliever, company and status symbol and addiction with duration and wanting to quit (p < 0.05). A high significance was observed in age with duration, expenditures, company, stress reliever and status symbol (p < 0.01). Frequency of consumption with addiction, and stress reliever (p < 0.01); stress reliever with age (p < 0.01); addiction with stress reliever and status symbol (p < 0.01); wanting to quit with gender and duration (p < 0.01); and lastly expenditures with encouragement, addiction, status symbol and stress reliever (p < 0.01) were also observed in 2-tailed significance of Pearson’s correlation. This study concludes that shisha smoking is responsible for a lot of ill-effects in the oral cavity apart from the body itself. This habit should be minimized and the misconceptions related to it should be made clear to all young population to avoid further harm to their health.
Protective Vaccination Against Primary And Recurrent Disease Caused By Herpes Simplex Virus (HSV) Type 2 Using A Genetically Disabled HSV-1

: The vaccine potential of a mutant herpes simplex virus (HSV) type 1, with a deletion in the glycoprotein H (gH) gene, was evaluated. The virus requires a gH-expressing cell line for multi-cycle growth but can complete a single cycle of infection in noncomplementing cells. Such viruses, termed DISC (disabled infectious single cycle) viruses, should be safe, yet still able to stimulate humoral and cell-mediated responses against a broad range of virus antigens in vaccinated hosts. Prophylactic vaccination of guinea pigs with DISC HSV-1, by ear scarification or direct infection of the vaginal mucosa, afforded a high degree of protection against HSV-2-induced primary genital disease and reduced significantly the frequency of subsequent disease recurrence. There was also a trend toward reduced recurrence following therapeutic vaccination of animals already infected with HSV-2. DISC HSV vaccination, therefore, offers an effective route for control of HSV disease.
Machine perfusion in kidney transplantation: clinical application & metabolomic analysis

Kidney Transplantation is the gold standard treatment for patients with end-stage renal failure. Most kidneys used for transplantation are from deceased donors and ensuring successful outcomes depends on many factors. One of these is organ storage. Hypothermic Machine Perfusion (HMP) of deceased donor organs has been shown to have several benefits. However, it has not been widely adopted and the underlying mechanism is poorly understood. The first section of this thesis examines the introduction of HMP into clinical practice. HMP outcomes were similar to those of standard storage techniques but with the additional benefit of increasing safe storage times. This was likely due to inherent benefits of the machine itself, improved recipient preparation and better peri-operative conditions. The second part of this study analysed HMP perfusate using metabolomics (Nuclear Magnetic Resonance) to identify potential predictors of graft outcome. Differences were identified in the metabolic profiles of perfusate from kidneys with immediate and delayed graft function. These may have a future role in viability assessment. Improved understanding of metabolism during storage may help target optimization strategies for deceased donor organs. The final part of this study describes the development of a porcine model of transplantation to test future hypotheses.
In Silico molecular docking analysis to identify PI3K inhibitors as possible NSCLC agents

Phosphatidylinositol 3-kinase is a very promising anti cancer drug target which is being actively investigated for treatment of various cancers such as RCC, prostrate, NSCLC and solid tumors. In this article, we have made an attempt to study the role of PI3K pathway inhibitors in the treatment of lung cancer through Insilco Molecular Docking studies. It was indicated from our study that the docking protocol could reliably predict how PI3K inhibitors act in NSCLC. The docking study carried out with 1M17 revealed that all six PI3K inhibitors have potential to work for NSCLC, but GDC0941 (-7.03 kcal/mol) and GSK 2126458 (-8.30 kcal/mol) fits better than the rest.
Guía para la secuencia de inducción e intubación rápida en el servicio de emergencias

La secuencia de intubacion rapida (SIR) es un procedimiento disenado para minimizar el tiempo necesario en el aseguramiento de la via aerea mediante la colocacion de un tubo endotraqueal en pacientes con alto riesgo de broncoaspiracion, especialmente en situaciones de emergencia. Teniendo claro este panorama, es indiscutible la importancia de la educacion y el entrenamiento relacionado con la secuencia de intubacion rapida que debe hacerse a los medicos responsables de las salas de reanimacion, los servicios de urgencias y los paramedicos responsables del manejo de campo en emergencias y desastres. Este documento es una revision actualizada del tema, cuyo objetivo es servir de guia para todos quienes esten interesados
A New Design Methodology for Composing Complex Digital Systems

Continuous growth in the use of multimedia applications on portable devices makes the mobile computer systems have an increasing complexity. The functionalities of the used SOC chips and silicon intelligent properties in these portable devices are become complicated and hard to design. Traditional digital circuit designs adopt register transfer level with timing control methodologies, which focus on the datapath composition, timing control of registers, and the functions of combinational circuits. However, the huge amount of control and synchronous signals of the above components are difficult to design and debug. The timing costs of design and verification are increased dramatically. This paper proposed a new design methodology of digital system, called data-oriented methodology, to deal with the above problems, by using Bluespec SystemVerilog HDL and the corresponding tools. Instead of conventional timingcontrol mechanism, the data-oriented methodology adopts simple handshaking protocol, blocking transferring, and explicitly register/FIFO declaration for communicating between adjacent modules. The designs of FDCT/IDCT and pipelined MIPS-like CPU are adopted to compare the design costs of conventional timing-control and data-oriented methodologies. The chip performance and FPGA proven of these two designs are discussed
Physical Activity during School Recess: The Liverpool Sporting Playgrounds Project

Recess offers primary school age children the opportunity to engage in physical activity, though few studies have detailed the physical activity levels of children in this environment. The physical activity levels of 270 children ages 6-11 years from 18 schools were monitored on 1 school day using heart rate telemetry. Data revealed that boys engaged in higher levels of moderate-to-vigorous and vigorous physical activity (MVPA) than did girls during recess (26 and 20 min, respectively). These results suggest that recess can make a worthwhile contribution to the recommended 60 min of MVPA per day.
Context Focused Intelligence Service within Big Data Environment: Knowledge Enabled Activeness, Efficiency and Precision

Big Data causes the problem of “Information Overwhelming”, which makes traditional intelligence service no longer effective. Required intelligence is submerged by huge amount of noise data, thus difficult to locate. The only way to get it is by keyword-based search, which however, brings down the efficiency and precision of intelligence service. To solve the problem, a domain knowledge based method is proposed. Firstly, context elements are defined to model commander decision task, and the mapping relations from commander decision task to commonly required intelligence are defined as domain knowledge. Then in running phases, commander decision task is monitored, and keyword expressions are automatically generated by applying the according pieces of knowledge. From user view, search results are provided actively along his decision making process. Moreover, it is proved by experiments that intelligence service efficiency and precision is improved by this method.
Recurrent Campylobacter Bacteremia as the First Manifestation of Hypogammaglobulinemia: a Case Report and Literature Review

A 30-year-old woman with a past medical history of autoimmune hemolytic anemia presented with fever. Blood cultures grew Campylobacter. Her medical history was significant for four prior episodes of Campylobacter gastroenteritis and bacteremia. She received ciprofloxacin for the index presentation, then Meropenem de-escalated to doxycycline 6 months later following recurrence of Campylobacter. This prompted investigation for an immunodeficiency disorder. She was found to have hypogammaglobulinemia. Her Campylobacter infections resolved following the administration of intravenous immunoglobulins every 3 weeks. She did not have recurrence of Campylobacter during 5 years of follow-up. A literature search revealed additional four case reports of six hypogammaglobulinemic adult individuals presenting with recurrent Campylobacter infections. Three patients were already on intravenous immunoglobulin (IVIG) when Campylobacter infection occurred, and two patients achieved clinical cure following therapy with imipenem and IVIG. This case report highlights the importance of suspecting hypogammaglobulinemia in patients with recurrent Campylobacter infections, as this is sometimes the first manifestation of the condition.
Effect of laser treatment on postural control parameters in patients with chronic nonspecific low back pain: a randomized placebo-controlled trial

The management of nonspecific lumbar pain (NSLP) using laser irradiation remains controversial. A systematic review of recently published studies indicates that the effects of laser therapy are commonly assessed using only imperfect methods in terms of measurement error. The main objective of this study was to assess static postural stability using an objective tool in patients with chronic NSLP after laser irradiation at different doses and wavelengths. In total, 68 patients were included in the laser sessions and were randomly assigned into four groups: high-intensity laser therapy at 1064 nm and 60 J/cm2 for 10 min (HILT), sham (HILT placebo), low-level laser therapy at 785 nm and 8 J/cm2 for 8 min (LLLT), and sham (LLLT placebo). In addition, all patients were supplemented with physical exercises (standard stabilization training). To assess postural stability, a double-plate stabilometric platform was used. All measurements were performed pre- and post-laser sessions (three weeks) and at follow-up time points (one and three months). Laser procedures led to more balanced posture stability in patients, although these positive changes were significant mainly for short-term observation (after 4-week therapy). In the follow-up analysis, the parameters were gradually impaired. Kruskal-Wallis analysis of variance (ANOVA) for independent variables did not show any difference between the studied groups. Low- and high-intensity laser therapy does not lead to a significant improvement in postural sway in patients with NSLP compared with standard stabilization training based on short- and long-term observations.