Miracle-dz/UniFAR-FTUs huggingface dataset:
Novel proteomic approach (PUNCH-P) reveals cell cycle-specific fluctuations in mRNA translation

Monitoring protein synthesis is required to understand gene expression regulation. Aviner et al. developed a system-wide proteomic approach for direct monitoring of translation, termed puromycin-associated nascent chain proteomics (PUNCH-P), which is based on incorporation of biotinylated puromycin into newly synthesized proteins followed by streptavidin affinity purification and LC-MS/MS analysis. Using PUNCH-P, cell cycle-specific fluctuations in synthesis for >5000 proteins were measured in mammalian cells. This approach also identified proteins not previously implicated in cell cycle processes and proteins that were not detected using other methods.
Spectral Energy Distributions and Age Estimates of 78 Star Clusters in M33

In this third paper of our series, we present CCD spectrophotometry of 78 star clusters that were detected by Chandar, Bianchi, & Ford in the nearby spiral galaxy M33. CCD images of M33 were obtained as a part of the BATC Multicolor Sky Survey of the sky in 13 intermediate-band filters from 3800 to 10000 Å. By aperture photometry, we obtain the spectral energy distributions of these 78 star clusters. As Chandar, Bianchi, & Ford did, we estimate the ages of our sample clusters by comparing the photometry of each object with theoretical stellar population synthesis models for different values of metallicity. We find that the sample clusters formed continuously in M33 from ∼3 × 106 to 1010 yr. This conclusion is consistent with Chandar, Bianchi, & Ford. The results also show that there are two peaks in cluster formation at ∼8 × 106 and ∼109 yr in these clusters.
Gait Event Anomaly Detection and Correction During a Split-Belt Treadmill Task

During instrumented split-belt treadmill tasks, it is challenging to avoid partially stepping on the contralateral belt. If this occurs, accurate detection of gait events from force sensors becomes impossible, as the force data are invalidated. In this paper, we present an algorithm, which automatically detects these invalid force data using an acceleration derivative-based measure. We used this algorithm in combination with the coordinate-based treadmill algorithm to replace the invalidated gait events detected from force sensors with those detected from 3-D markers. The performance of the proposed algorithm was evaluated against the visual examination of data collected from healthy participants in both the same speed and differential speed configurations, using the receiver operator characteristics, the area under the curve, and the Youden index. We found that the area under the curve (AUC) score was above 0.8 in both the same speed and differential speed configurations. Moreover, there was not enough evidence ( $p>0.05$ ) to suggest a correlation between walking speed and the performance of the algorithm. We conclude that the algorithm has good to excellent detection and correction performance, which can be useful for research involving analysis of gait with instrumented split-belt treadmills. A MATLAB (MathWorks, Inc., Natick, MA, USA) based implementation of the proposed algorithm and example data files are also presented.
Needs assessment in genetic testing education: A survey of parents of children with autism spectrum disorder in the united states

Understanding parents' educational needs concerning genetic testing for their children with autism spectrum disorder (ASD) is important in developing tailored, evidence‐based health education materials for clinical use. Since research is lacking in this area, to bridge the gap, we examined genetic testing education needs using a nationwide sample of parents of biological children with ASD in the United States. Prospective participants were recruited from the interactive autism network, and 552 parents of biological children with ASD completed the online survey. Most participants (73.7%) were interested in receiving health education about genetic testing. Yet, the majority of them (64.7%) reported that they did not receive the information needed from physicians. Parents who identified as racial/ethnic minorities (P = 0.029), who had an education degree below college (P = 0.002), or displayed low/no awareness of genetic testing (P = 0.003) were more interested in receiving health education regarding genetic testing. Parents' most desired topics for health education include the accuracy of genetic testing (88.4%), cost (85.9%), relevant benefits of such testing (83.8%), testing procedure (77.8%), eligibility to undergo genetic testing for their children with ASD (62.4%), potential harms caused by genetic testing (56.1%), previous use and experience among individuals affected by ASD (50.8%), and confidentiality issues (48.0%). Furthermore, web‐based education was the preferable approach (85.4%). Our findings can help develop health education programs and/or materials regarding genetic testing for parents and physicians to facilitate better physician‐parent communication and assist parents in making informed medical decisions regarding genetic testing. Autism Res 2019, 12: 1162–1170. © 2019 International Society for Autism Research, Wiley Periodicals, Inc.
Crosstalk between soluble PDGF‐BB and PDGFRβ promotes astrocytic activation and synaptic recovery in the hippocampus after subarachnoid hemorrhage

Platelet‐derived growth factor receptor β (PDGFRβ) dynamically changes after brain injury, possibly mediating the neuroprotective role of soluble homodimers of the platelet‐derived growth factor β subunit (PDGF‐BB) that is secreted by microcirculation cells. The aim of this study was to determine whether binding of PDGF‐BB to astrocytic PDGFRβ enhanced crosstalk among the various components of the neurovascular unit, leading to synaptic recovery after subarachnoid hemorrhage (SAH). The soluble PDGF‐BB from the cerebrospinal fluid (CSF) of patients with SAH was measured. The relationship between PDGF‐BB treatment and astrocytic PDGFRβ signaling was further explored in vivo and in vitro in experimental SAH models. Compared with the levels in the control samples, the PDGF‐BB protein levels in the CSF of patients with SAH were significantly increased. After the generation of experimental SAH, astrocyte activation markers were markedly induced by the binding of PDGF‐BB to astrocytic PDGFRβ, accompanied by improved levels of synaptic recovery and cognitive function. Soluble PDGF‐BB and astrocytic PDGFRβ signaling are essential for the neuroprotective effect in the hippocampus and the coculture system in vitro after SAH that otherwise leads to cognitive dysfunction and neuronal damage. —Zhou, X. , Wu, Q. , Lu, Y. , Zhang, X. , Lv, S. , Shao, J., Zhou, Y. , Chen, J., Hou, L. , Huang, C. , Zhang, X. Crosstalk between soluble PDGF‐BB and PDGFRβ promotes astrocytic activation and synaptic recovery in the hippocampus after subarachnoid hemorrhage. FASEB J. 33, 9588–9601 (2019). www.fasebj. org
Placement of Brånemark implants in the maxillary tuber region: anatomical considerations, surgical technique and long-term results.

BACKGROUND Fixture placement in the tuber area is one way to overcome the problem of insufficient bone volume for routine implant surgery in the posterior maxilla due to severe resorption of jawbone and an extensive enlargement of the maxillary sinus. However, little is known about the long-term results. PURPOSE The aim of this study was to retrospectively evaluate the survival rate and marginal bone conditions at fixtures placed in the tuber region of the maxilla. MATERIAL AND METHODS Twenty-one patients previously treated with at least one implant in the tuber region of the maxilla were included in this retrospective analysis. A total of 23 standard Brånemark System fixtures with a turned surface had been surgically placed in the tuber regions and 71 additional implants in adjacent areas to support fixed dental bridges. All implants were allowed to heal for 6-8 months before abutment connection and following prosthetic treatment. The patients were radiographed after 1-12 years for evaluation of marginal bone levels. In addition, the relation between the apex of the fixture in the tuberosity area and the posterior border of the maxilla was measured. RESULTS Twenty of the 21 patients representing 22 tuber and 64 additional implants were radiographically evaluated. No implants in the tuber areas were lost during the follow-up whereas two fixtures in the anterior region had to be removed, one before loading and the other after 4 years of loading not interfering with the prosthesis stability. The mean marginal bone level at tuber implants was situated on average 1.6 mm (SD 1.1, n=22) from the abutment-fixture junction, whilst the other implants showed an average bone level of 1.9 mm (SD 0.8, n=64). The results were similar when comparing partially and totally edentulous patients. CONCLUSION The present retrospective study shows good clinical outcome with standard Brånemark fixtures placed in the tuber region of the posterior maxilla using a two-stage procedure. In appropriate cases where bone of adequate volume and density is available, our data indicate that the technique can be used as an alternative to more extensive surgery and especially to the sinus lift procedure. However, prospective comparative studies are needed in order to evaluate the efficacy of the described technique for this purpose.
Tea catechin, epigallocatechin‐3‐gallate suppresses myosin II regulatory light chain phosphorylation

Phosphorylation of myosin II regulatory light chain (MRLC) is critical event for many cellular processes including muscle contraction, mytosis, migration, and exocytosis. Epigallocatechin‐3‐O‐gallate (EGCG) is a major polyphenolic compound of green tea and has various physiological functions. We found that EGCG disrupted stress fibers and suppressed the MRLC phosphorylation in HeLa cells. To elucidate the mechanism for the suppressive effect on the phosphorylation, we examined the effect of various inhibitors for kinases that modulate MRLC phosphorylation. None of the inhibitors mimic the activity of EGCG. These results suggest that EGCG is a compound that can suppress MRLC phosphorylation.
Plasma Apelin is Lower in Patients with Elevated LDL-Cholesterol

Hypercholesterolemia is a major risk factor for atherosclerosis. Dysregulation of adipokines contribute to atherosclerotic diseases. Apelin has recently been shown to be secreted by the adipose tissue in association with hyperinsulinemia and inflammation. We searched plasma apelin levels in patients with elevated low density lipoprotein (LDL)-cholesterol having no additional disorder. Thirty-three patients with hypercholesterolemia and 50 age-, sex-, and body mass index-matched healthy controls were evaluated for their apelin, adiponectin and high sensitivity C-reactive protein (hsCRP) levels, and homeostasis model assessment (HOMA) indexes. Plasma apelin-12 and adiponectin were determined by ELISA and RIA, respectively. Plasma apelin levels were lower in patients with elevated LDL-cholesterol compared to healthy controls (p<0.001). Plasma adiponectin concentration was also lower in the dyslipidemic patients (p<0.001). hsCRP levels were similar in the two groups. Fasting plasma glucose was normal in both groups. HOMA indexes in the dyslipidemic group were higher than the controls (p=0.005). A mild to moderate negative correlation with HOMA and positive correlation with high density lipoprotein cholesterol of apelin was found in the dyslipidemic group. Plasma apelin is decreased in non-obese, non-diabetic and normotensive patients with elevated LDL-cholesterol. Low apelin levels in hypercholesterolemia seem associated with insulin resistance, which needs to be investigated in larger populations as well as in other atherosclerotic conditions.
Factors affecting Breslow thickness: Results from a survey of 114 Western Australian patients with thicker melanoma

Detecting melanoma in the earlier stages of the disease reduces mortality. Breslow thickness is an important determinant of prognosis. In Western Australia, between 2000 and 2011, the median Breslow thickness of melanomas increased. Previous studies have shown a positive association between age and male gender, and Breslow thickness. Lesion characteristics, such as the anatomical location, subjective lesion changes and the amelanotic melanoma subtype, have been associated with Breslow thickness. Our study sought to understand the characteristics of patients presenting with thicker melanoma. These included personal factors, such as general demographics and the visibility of the culprit lesion, and health-care experiences prior to diagnosis. We also sought to compare patients with non-pigmented lesions to those with pigmented lesions. Over a 2-year period, patients with melanoma, who presented for follow-up at a single melanoma service in Western Australia, were invited to complete a questionnaire whilst waiting for their appointment. The questionnaire contained 12 questions pertaining to demographics, lesion characteristics and health-care experiences. Patients reported their age as a continuous variable and selected responses from a set of options for all other questions. The surveys were matched to a pathology result for Breslow thickness. A descriptive summary was produced to identify shared characteristics. As a secondary outcome, patients with pigmented lesions and those with non-pigmented lesions were compared. Important limitations in the data collection methodology should be noted: time from diagnosis to response was not recorded; survey refusal rate not recorded; and nor was there any external validation of the survey. SPSS version 23 was used for statistical analysis. Chisquared tests assessed the association between categorical variables. Independent samples t-tests compared continuous variables between groups. Post hoc analysis ensured adequate power, for a large effect size, for comparisons between the pigmented and non-pigmented groups. This study was approved by the St John of God Health Care Human Research Ethics Committee (ref: 746). All patients provided informed consent. One hundred and fourteen patients completed the questionnaire. Responses are summarised in Table 1. The mean age was 62 years (SD = 16). Sixty per cent of the cohort
Draft Genome Sequence of Streptomyces thermocarboxydus BPSAC147, a Potentially Plant Growth-Promoting Endophytic Bacterium

Streptomyces thermocarboxydus strain BPSAC147 is an endophytic actinobacterium isolated from root tissues of Rhynchotechum ellipticum in Mizoram, Northeast India. The strain showed potentially plant growth-promoting and biocontrol activities. ABSTRACT Streptomyces thermocarboxydus strain BPSAC147 is an endophytic actinobacterium isolated from root tissues of Rhynchotechum ellipticum in Mizoram, Northeast India. The strain showed potentially plant growth-promoting and biocontrol activities. The draft genome consists of 7.37 Mb and encodes 7,060 putative open reading frames.
A Sociotechnical Approach to Successful Electronic Health Record Implementation: Five Best Practices for Clinical Nurse Specialists

Rising healthcare costs coupled with patient safety considerations and quality of care have become major concerns for healthcare purchasers, providers, and policymakers. Health information technology, particularly the electronic health record (EHR), is posed as a solution to address these concerns by delivering greater efficiencies and improved quality of care. Despite the national movement toward EHR adoption, successful EHR implementation continues to be challenging for many healthcare organizations, both large and small. This article uses sociotechnical systems theory as a framework to discuss 5 best practice guidelines for EHR implementation and outlines what clinical nurse specialists can do to make the process successful.
Temperature-Sensitive Reproduction and the Physiological and Evolutionary Potential for Mother’s Curse

Abstract Strict maternal transmission of mitochondrial DNA (mtDNA) is hypothesized to permit the accumulation of mitochondrial variants that are deleterious to males but not females, a phenomenon called mother’s curse. However, direct evidence that mtDNA mutations exhibit such sexually antagonistic fitness effects is sparse. Male-specific mutational effects can occur when the physiological requirements of the mitochondria differ between the sexes. Such male-specific effects could potentially occur if sex-specific cell types or tissues have energy requirements that are differentially impacted by mutations affecting energy metabolism. Here we summarize findings from a model mitochondrial–nuclear incompatibility in the fruit fly Drosophila that demonstrates sex-biased effects, but with deleterious effects that are generally larger in females. We present new results showing that the mitochondrial–nuclear incompatibility does negatively affect male fertility, but only when males are developed at high temperatures. The temperature-dependent male sterility can be partially rescued by diet, suggesting an energetic basis. Finally, we discuss fruitful paths forward in understanding the physiological scope for sex-specific effects of mitochondrial mutations in the context of the recent discovery that many aspects of metabolism are sexually dimorphic and downstream of sex-determination pathways in Drosophila. A key parameter of these models that remains to be quantified is the fraction of mitochondrial mutations with truly male-limited fitness effects across extrinsic and intrinsic environments. Given the energy demands of reproduction in females, only a small fraction of the mitochondrial mutational spectrum may have the potential to contribute to mother’s curse in natural populations.
Two-dimensional Photoelectric Compensation based on BP Neural Network

To improve the accuracy of heading angle measured by electronic compass, a new error compensation method is proposed based on adaptive differential evolution algorithm and BP neural network. In the method, the 3-layer BP neural network is used to model heading angle error, and adaptive differential evolution algorithm is adopted to train the weights of network, thus obtaining a more exact error model, and compensating the heading angle error measured by electronic compass. This method is compared with eight position least square, BP neural network, differential evolution algorithm, BP neural network optimization and other compensation methods. The accuracy of error compensation is improved obviously, and it has the characteristics of strong global optimization ability, fast convergence speed and good stability. The experimental results show that after compensation, the error range of heading angle is decreased from $-16^{0}\sim 30.7^{0}$ to $-0.22^{o} \sim 0.2^{0}$, which satisfies the needs of higher accuracy navigation systems.
Analytical Model for RDF-Induced Threshold Voltage Fluctuations in Double-Gate MOSFET

In this paper, a physics-based threshold voltage model of symmetrical double gate (DG) MOSFET, including the random dopants in the channel is presented. The model is derived from the solution of 2-D Poisson’s equation and is suitable for circuit simulation. The average potential considering random dopants in the channel is used to calculate the threshold voltage and provides accurate results. The developed threshold voltage model is validated with TCAD simulations for different device dimensions and doping concentrations. The standard deviation (<inline-formula> <tex-math notation="LaTeX">${\sigma } {\text{V}}_{\text{TH}}$ </tex-math></inline-formula>) is calculated from a threshold voltage distribution of 200 devices. The proposed model is useful to simulate variations in a large number of devices with randomly placed dopants, with less computational time. The model is integrated in the Cadence circuit simulator and analyzed the effect of random dopant fluctuation-induced <inline-formula> <tex-math notation="LaTeX">${\text{V}}_{\text{TH}}$ </tex-math></inline-formula> variations of n-channel DG MOSFET in the inverter circuit.
Programming Behavioral Test Models for SMT Solving in Scala

We present a novel approach for modeling cyber-physical systems for analysis and test purposes. Instead of creating a new expressive specification language with sophisticated semantics and complex compilers, we rely on a lightweight version of Back's Action Systems, for which we provide a simple bounded model checker using the SMT solver Z3. In order to model industrial-sized embedded systems, we extend our simple specification language by using the powerful capa-bilities of the modern programming language Scala for creating Domain Specific Languages (DSL). This enables us to use the features of an expressive, object-oriented and functional generalpurpose language without the need to increase the complexity of the model checker. We demonstrate how to model a railway interlocking system with a configurable track layout and sketch the application to model-based testing.
Important Considerations for Testing and Commissioning Digital Protective Relays: Avoid Misoperations

The proven advantages of digital technology for power system protective relays are now commonplace in the power producing and delivery industry. Digital relays provide unsurpassed reliability and extended capabilities at an economical cost. Keeping pace with the testing and commissioning requirements of these devices has proven to be a challenge for both protective relay engineers and technicians. Although testing procedures have been well defined for single-function electromechanical (EM) protection devices, modern relay test procedures have been left to the utility to develop, creating possible shortcuts that may compromise the protection system operation.
Detecting Surrounding Users by Reverberation Analysis with a Smart Speaker and Microphone Array

Recently, smart speakers like Amazon Echo and Google Home have been spread widely. Those devices support users' life through voice interface by receiving voice commands to operate appliances and order goods to online shops. Meanwhile, it is reported that smart speakers are vulnerable to some malicious attacks which steal personal information and/or order unnecessary goods by uttering voice from a device nearby the speaker, abusing the fact that the smart speakers cannot distinguish human voice from machine voice. A new type of attack called DolphinAttack which utters ultrasonic voice inaudible to human is also reported. Therefore, a method to identify which of human or machine is sending voice commands to a smart speaker is desired. In this paper, to prevent such machine-voice based attacks to a smart speaker in absence of residents, we propose a system consisting of a speaker and a microphone array to detect the existence of a human nearby, supposing it can be incorporated in a smart speaker in the future. In our proposed system, the speaker emits sonar sound generated based on Orthogonal Frequency Division Multiplexing (OFDM) in all directions, the microphone array with 8 channels attached on top of the speaker receives the reflected sound, and the human existence is judged by comparing the reflected sound with that measured in the same environment without human. Through experiments with a prototype system, we confirmed that our proposed system can detect the human existence by measuring the reflected signal of 0.5 second.