Miracle-dz/UniFAR-FTUs huggingface dataset:
Bifunctional Anti-Human Immunodeficiency Virus Type 1 Small Molecules with Two Novel Mechanisms of Action

ABSTRACT A class of betulinic acid derivatives was synthesized to target two critical steps in the human immunodeficiency virus type 1 (HIV-1) replication cycle, entry and maturation. Each mechanism of HIV-1 inhibition is distinct from clinically available anti-HIV therapeutics. The viral determinants of the antientry and antimaturation activities are the bridging sheet of HIV-1 gp120 and the P24/p2 cleavage site, respectively.
EBNA-2-Deleted Epstein-Barr Virus from P3HR-1 Can Infect Rabbits with Lower Efficiency than Prototype Epstein-Barr Virus from B95-8

Objectives: To clarify characteristics on rabbit in vivo infection with type 2 EBV nuclear antigen (EBNA-2)-deleted Epstein-Barr virus (P3HR-1-EBV) and compare infectious efficacy of P3HR-1-EBV with previously reported prototype type 1 EBV from B95-8. Methods: Twelve Japanese White rabbits were inoculated with P3HR-1-EBV via intranasal or intravenous routes and autopsied on day 70–84. Results: In only 2 of 12 P3HR-1-EBV-inoculated rabbits, EBV-DNA was detected in peripheral blood mononuclear cells (PBMCs). BamHI M rightward reading frame (BMRF)-1, EBNA-1 and BamHI Z leftward reading frame (BZLF)-1-mRNA were intermittently expressed in PBMCs. In 1 infected rabbit with continuous detection of EBV-DNA in PBMCs, many EBER-1-positive lymphocytes were observed in germinal centers and/or marginal zones in some follicles of the appendix, and for the first time a lymphocyte with EBER-1 expression infiltrating in the squamous cell layer of the tonsils was found. The other rabbit with a transient detection of EBV-DNA in PBMCs had no EBER-1-positive lymphocytes in the tissues examined. Few EBER-1-positive lymphocytes were detected in some rabbits without detection of EBV-DNA in PBMCs. Conclusions: P3HR-1-EBV showed less efficient infection in rabbits than EBV from the B95-8 cell line. However, a P3HR-1-EBV-inoculated animal model is meaningful because this is the first study of EBNA-2 function on in vivo EBV infection and it demonstrated the in vivo infectivity with lytic-type infection by EBNA-2-deleted EBV.
The Perception of English Intonation Patterns by German L2 Speakers of English

Previous research suggests that intonation is a particularly challenging aspect of L2 speech learning. While most research focuses on speech production, we widen the focus and study the perception of intonation by L2 learners. We investigate whether advanced German learners of English have knowledge of the appropriate English intonation patterns in a narrative context with different sentence types (e.g. statements, questions). The results of a tonal pattern selection task indicate that learners (n=20) performed similar to British English controls (n=25) for some sentence types (e.g. statements, yes/no-questions), but performed significantly worse than the control group in the case of open and closed tag questions and the expression of sarcasm. The results can be explained by the fact that tag questions are the only sentence type investigated that does not exist in the learners' L1, and sarcasm is not represented syntactically. This suggests that L1 influence can partly account for why some intonation patterns are more challenging than others, and that contextualized knowledge of the intonation patterns of the target language rather than knowledge of intonation patterns in isolation is crucial for the successful L2 learning of intonation.
Novel Functional Imaging of Changes in Small Airways of Patients Treated with Extrafine Beclomethasone/Formoterol

Background: Inhaled formulations using extrafine particles of long-acting β2-agonists and corticosteroids were developed to optimize asthma treatment. Findings that these combinations reach and treat smaller airways more effectively are predominantly based on general non-specific outcomes with little information on regional characteristics. Objectives: This study aims to assess long-term effects of extrafine beclomethasone/formoterol on small airways of asthmatic patients using novel functional imaging methods. Methods: Twenty-four stable asthma patients were subdivided into three groups (steroid naive, n = 7; partially controlled, n = 6; well controlled, n = 11). Current treatment was switched to a fixed combination of extrafine beclomethasone/formoterol (Foster®; Chiesi Pharmaceuticals, Parma, Italy). Patients underwent lung function evaluation and thorax high-resolution computerized tomography (HRCT) scan. Local airway resistance was obtained from computational fluid dynamics (CFD). Results: After 6 months, the entire population showed improvement in pre-bronchodilation imaging parameters, including small airway volume (p = 0.0007), resistance (p = 0.011), and asthma control score (p = 0.016). Changes in small airway volume correlated with changes in asthma control score (p = 0.004). Forced expiratory volume in 1 s (p = 0.044) and exhaled nitric oxide (p = 0.040) also improved. Functional imaging provided more detail and clinical relevance compared to lung function tests, especially in the well-controlled group where only functional imaging parameters showed significant improvement, while the correlation with asthma control score remained. Conclusions: Extrafine beclomethasone/formoterol results in a significant reduction of small airway obstruction, detectable by functional imaging (HRCT/CFD). Changes in imaging parameters correlated significantly with clinically relevant improvements. This indicates that functional imaging is a useful tool for sensitive assessment of changes in the respiratory system after asthma treatment.
Genomic analysis of RNA alternative splicing in cancers

Alternative splicing is an important and prevalent mechanism of gene regulation in higher eukaryotes. Nearly three quarters of human multi-exon genes are alternatively spliced. There is great interest in discovering alternative splicing events in the transcriptome of cancer cells, and in understanding how alternative splicing contributes to tumorigenesis. In this article, I will review recent advances in global analyses of pre-mRNA alternative splicing, and the applications of these genomic technologies to studies of alternative splicing in human cancers.
ADABCAST: Adaptive broadcast approach for solar Energy Harvesting Wireless Sensor Networks

The problem of message broadcasting from the base station (BS) to sensor nodes (SNs) in solar energy harvesting wireless sensor networks (EHWSN) is considered in this paper. The aim is to ensure fast and reliable broadcasting without interfering with upstream communications (from SNs to BS), whilst taking into account energy harvesting constraints. An adaptive approach is proposed where the BS first selects the broadcast time slots, given a wake-up schedule for the SNs (the time slots where the SN are active and in receiving mode). Hence, the SNs adapt their schedules. This is then iterated seeking optimal selection of the broadcast time slots, so as to minimize broadcast overhead (transmitted messages) and latency. Our approach enables fast broadcast and eliminates the need for adding protocol overhead (redundancy), compared to the existing solutions. Hidden Markov Model (HMM) and Baum-Welch learning algorithm are used for this purpose. Numerical results confirm that our scheme performs the broadcast operation in less time, and by reducing the broadcast overhead, as compared to state-of-the-art approaches.
Research on a language for commanding and controlling multi-robot systems

Nowadays, many military missions can profit from using robots. It seems that the multi-robot forces would become the main strength of an army just around the corner. However, besides the achievements of the interaction between human and robots, there are still many problems to be solved, such as the collaboration among robots or the intelligent information fusion between human and robotic systems. With the aim of solving these problems, a language called Situation Information Exchange and Interpretation Language (SIEIL) is proposed. The technical framework, formalized syntax, semantic annotation method and communication protocol of the language are key techniques in this paper. In the end of this paper, a usecase of naval warfare illustrates the feasibility and the effectiveness of this language.
Jorge Jordana: secretario general de la federación española de industrias de la alimentación y bebidas (FIAB)

Jorge Jordana se muestra preocupado por los problemas que pude acarrear para los proveedores la fusion en el sector de la distribucion. A su juicio, habria que hacer unos cambios en la ley de Comercio para evitar los abusos, y cree necesarias las fusiones dentro de la industria agroalimentaria.
Pathophysiology of nerve compression syndromes: response of peripheral nerves to loading.

Nerve compression syndromes involve peripheral-nerve dysfunction that is due to localized interference of microvascular function and structural changes in the nerve or adjacent tissues. Although a well known example is compression of the median nerve at the wrist (carpal tunnel syndrome), other nerves, such as the ulnar nerve at the wrist or the elbow and the spinal nerve roots at the vertebral foramen, are vulnerable. This paper focuses on studies in which the physiological, pathophysiological, biochemical, and histological effects of biomechanical loading on the peripheral nerves were evaluated in humans and animals. When tissues are subjected to load or pressure, they deform and pressure gradients are formed, redistributing the compressed tissue toward areas of lower pressure. Nerve compression syndromes usually occur at sites where the nerve passes through a tight tunnel formed by stiff tissue boundaries. The resultant confined space limits movement of tissue and can lead to sustained tissue pressure gradients. Space-occupying structures or lesions (for example, lumbrical muscles, tumors, and cysts) can cause nerve compression injury, as can conditions associated with accumulation of fluid (for example, pregnancy, congestive heart failure, and muscle compartment syndromes) or accumulation of extracellular matrix (for example, acromegaly, myxedema hypothyroidism, and mucopolysaccharidosis)76. Although nerve injuries related to vibration occur near the region of exposure, the symptoms may be manifest at another site, where the nerve may be constricted. Other conditions, such as diabetes mellitus, may increase the likelihood that a compressed nerve will undergo a pathological response. In addition, there may be an inflammatory reaction that may impair the normal gliding of the nerve. Basic knowledge of the microanatomy of peripheral nerves and neurons and of their complex reactions to compression is essential to understanding, preventing, and treating nerve compression injuries. ### Microanatomy The neuron consists of the nerve cell body, located in the …
A qualitative study of the childbearing experience of women living with multiple sclerosis

Purpose: Although at any time in the UK, there are about 20,000 women with MS who may be considering having children, healthcare system often fails to provide them with information and support they need to make informed decisions about their health and pregnancy management. The aim of this paper is to explore the childbearing experience of women with MS to determine what support and information may be useful to this target group. Method: Interviews were conducted with women with MS (n = 9). Transcripts were analysed using thematic analysis. Results: Three major themes emerged from the interviews with women living with MS. We found women were concerned about both medical and practical issues associated with having children. Limited access to information about relationships between MS and childbearing and receiving conflicting or wrong information was recounted. Opinions of family members and clinicians regarding having children in the context of MS impacted on women’s experience of making decision about having children and childbearing. Conclusions: Women with MS can benefit from having access to comprehensive, structured sources of information about MS and childbearing. Healthcare professionals and family members ‘support could be channelled more appropriately to enhance their experience of making choices about childbearing. Implications for Rehabilitation Women with MS who are considering having children express a number of medical and practical concerns, but they experience difficulty in accessing to comprehensive and reliable sources of information about MS and childbearing. We recommend developing a resource offering women living with MS and clinicians working with this group of patients comprehensive, credible and up to date information about different aspects of MS and childbearing. We recommend women with MS who are pregnant have access to a multidisciplinary team of clinicians available for consultation and ad hoc advice to address women’s concerns and make individualized plan for pregnancy and condition management.
Facilitation of human first dorsal interosseous muscle responses to transcranial magnetic stimulation during voluntary contraction of the contralateral homonymous muscle

The size of compound motor evoked potentials (cMEPs) to transcranial magnetic stimulation of the motor cortex was measured in the relaxed first dorsal interosseous muscle of the nondominant hand (ndFDI) during different levels of voluntary contraction in the homonymous muscle of the dominant hand (dFDI). cMEP responses in the ndFDI became larger when the dFDI was contracted to forces ranging 10–70% of maximum voluntary contraction. Variability in the amplitude of the cMEP responses in ndFDI decreased when dFDI was contracted. Comparison with cMEPs to spinal cord stimulation suggested a large component of the facilitation was occurring at a cortical level. The amplitude of cMEP responses in ndFDI also increased when the tibialis anterior muscle of the leg on the contralateral side was contracted. The observed facilitation of motoneurons during contraction of contralateral muscles might involve a transcallosal pathway modulating the excitability of one cortex when the other is activated. © 1998 John Wiley & Sons, Inc. Muscle Nerve 21:1033–1039, 1998
Effectiveness of pneumococcal conjugate vaccine against presumed bacterial pneumonia hospitalisation in HIV-uninfected South African children: a case–control study

Introduction We evaluated pneumococcal conjugate vaccine (PCV) effectiveness against hospitalisation for presumed bacterial pneumonia (PBP) in HIV-uninfected South African children. 7-valent PCV was introduced in April 2009 using a 2+1 schedule (doses at age 6, 14 and 39 weeks), superseded with 13-valent PCV in May 2011. Methods A matched case–control study was conducted at three public hospitals (Soweto, Cape Town and KwaZulu-Natal) between April 2009 and August 2012. PBP cases had either WHO defined radiographically confirmed pneumonia or ‘other infiltrate’ on chest radiograph with C-reactive protein ≥40 mg/L. Hospitalised controls were children admitted with a disease unlikely to be pneumococcal and matched for case age, site and HIV infection status. Age-matched community controls were enrolled from Soweto. Adjusted vaccine effectiveness (aVE) was estimated using conditional logistic regression. Results Of 1444 HIV-uninfected enrolled PBP cases, 1326 had ≥1 hospital controls (n=2075). Overall, aVE of an up-to-date PCV schedule was 20.1% (95% CI −9.3% to 41.6%) in children aged ≥8 weeks and 39.2% (95% CI 8.46% to 59.6%) among children 16–103 weeks of age. There were 889 PBP cases in Soweto with hospital controls and ≥1 community control (n=2628). The aVE using community controls was similar compared with hospital controls in Soweto, including 32.1% (95% CI 4.6% to 51.6%) and 38.4% (95% CI 7.7% to 58.8%), respectively, in age group ≥8 weeks and 52.7% (95% CI 25.7% to 69.9%) and 53.8% (95% CI 19.5% to 73.5%), respectively, in age group 16–103 weeks. Conclusions PCV implemented using a 2+1 schedule in the routine infant immunisation programme was effective at preventing PBP in HIV-uninfected children. Effectiveness estimates were similar to efficacy measured by earlier randomised controlled trials using different vaccination schedules.
Rapid detection of Mycobacterium avium in stool samples from AIDS patients by immunomagnetic PCR

Direct PCR detection of bacteria in clinical samples is often hindered by the presence of compounds that inhibit the PCR. To improve and accelerate the diagnosis of Mycobacterium avium-M. intracellulare complex infections, an immunomagnetic PCR (IM-PCR) assay was developed. This IM-PCR procedure combines the separation of mycobacteria by antimycobacterial monoclonal antibody coupled to magnetic beads with an M. avium-M. intracellulare complex-specific PCR protocol based on 16S rRNA gene sequences. As few as 10 M. avium bacilli were detected in spiked human stool samples, a clinical specimen usually refractory to conventional PCR analysis, by the IM-PCR method. Moreover, M. avium organisms were detected in about 24 h in 18 of 22 culture-confirmed fecal samples from AIDS patients. This IM-PCR protocol should allow for the rapid and sensitive detection of M. avium isolates in clinical specimens.
Receptor Number and Caveolar Co-localization Determine Receptor Coupling Efficiency to Adenylyl Cyclase*

Recent evidence suggests that many signaling molecules localize in microdomains of the plasma membrane, particularly caveolae. In this study, overexpression of adenylyl cyclase was used as a functional probe of G protein-coupled receptor (GPCR) compartmentation. We found that three endogenous receptors in neonatal rat cardiomyocytes couple with different levels of efficiency to the activation of adenylyl cyclase type 6 (AC6), which localizes to caveolin-rich membrane fractions. Overexpression of AC6 enhanced the maximal cAMP response to β1-adrenergic receptor (β1AR)-selective activation 3.7-fold, to β2AR-selective activation only 1.6-fold and to prostaglandin E2 (PGE2) not at all. Therefore, the rank order of efficacy in coupling to AC6 is β1AR > β2AR > prostaglandin E2 receptor (EP2R). β2AR coupling efficiency was greater when we overexpressed the receptor or blocked its desensitization by expressing βARKct, an inhibitor of G protein-coupled receptor kinase activation, but was not significantly greater when cells were treated with pertussis toxin. Assessment of receptor and AC expression indicated co-localization of AC5/6, β1AR, and β2AR, but not EP2R, in caveolin-rich membranes and caveolin-3 immunoprecipitates, likely explaining the observed activation of AC6 by βAR subtypes but lack thereof by PGE2. When cardiomyocytes were stimulated with a βAR agonist, β2AR were no longer found in caveolin-3 immunoprecipitates; an effect that was blocked by expression of βARKct. Thus, agonist-induced translocation of β2AR out of caveolae causes a sequestration of receptor from effector and likely contributes to the lower efficacy of β2AR coupling to AC6 as compared with β1AR, which do not similarly translocate. Therefore, spatial co-localization is a key determinant of efficiency of coupling by particular extracellular signals to activation of GPCR-linked effectors.
Computer-Aided Detection of Bleeding Regions for Capsule Endoscopy Images

Capsule endoscopy (CE) has been widely used to diagnose diseases in human digestive tract. However, a tough problem of this new technology is that too many images to be inspected by eyes cause a huge burden to physicians, so it is significant to investigate computerized diagnosis methods. In this paper, a new computer-aided system aimed for bleeding region detection in CE images is proposed. This new system exploits color texture feature, an important clue used by physicians, to analyze status of gastrointestinal tract. We put forward a new idea of chrominance moment as the color part of color texture feature, which makes full use of Tchebichef polynomials and illumination invariant of hue/saturation/intensity color space. Combined with uniform local binary pattern, a current texture representation model, it can be applied to discriminate normal regions and bleeding regions in CE images. Classification of bleeding regions using multilayer perceptron neural network is then deployed to verify performance of the proposed color texture features. Experimental results on our bleeding image data show that the proposed scheme is promising in detecting bleeding regions.
Goal!! Event detection in sports video

Understanding complex events from unstructured video, like scoring a goal in a football game, is an extremely challenging task due to the dynamics, complexity and variation of video sequences. In this work, we attack this problem exploiting the capabilities of the recently developed framework of deep learning. We consider independently encoding spatial and temporal information via convolutional neural networks and fusion of features via regularized Autoencoders. To demonstrate the capacities of the proposed scheme, a new dataset is compiled, composed of goal and no-goal sequences. Experimental results demonstrate that extremely high classiﬁcation accuracy can be achieved, from a dramatically limited number of examples, by leveraging pre-trained models with ﬁne-tuned fusion of spatio-temporal features.
Bullying Victimization Uniquely Contributes to Adjustment Problems in Young Children: A Nationally Representative Cohort Study

OBJECTIVE. It has been shown that bullying victimization is associated with behavior and school adjustment problems, but it remains unclear whether the experience of bullying uniquely contributes to those problems after taking into account preexisting adjustment problems. METHODS. We examined bullying in the Environmental Risk Study, a nationally representative 1994–1995 birth cohort of 2232 children. We identified children who experienced bullying between the ages of 5 and 7 years either as pure victims or bully/victims. We collected reports from mothers and teachers about children’s behavior problems and school adjustment when they were 5 years old and again when they were age 7. RESULTS. Compared with control children, pure victims showed more internalizing problems and unhappiness at school when they were 5 and 7 years. Girls who were pure victims also showed more externalizing problems than controls. Compared with controls and pure victims, bully/victims showed more internalizing problems, more externalizing problems, and fewer prosocial behaviors when they were 5 and 7 years. They also were less happy at school compared with control children at 7 years of age. Pure victims and bully/victims showed more behavior and school adjustment problems at 7 years of age, even after controlling for preexisting adjustment problems at 5 years of age. CONCLUSIONS. Being the victim of a bully during the first years of schooling contributes to maladjustment in young children. Prevention and intervention programs aimed at reducing mental health problems during childhood should target bullying as an important risk factor.
How to provide nutritional support of sick neonatal foals.

Foals are born with limited stores of glycogen and fat, and even short periods of starvation can result in profound hypoglycemia, weakness, and hypothermia. Accordingly, nutritional support of the sick neonatal foal is critical for a successful outcome. The initial goal of nutritional support is to meet resting energy and nutrient requirements. Nutrition can be provided by the enteral route, the parenteral (IV) route, or a combination of the two. Enteral feeding of mare’s milk through an indwelling nasogastric tube is the preferred mode of nutritional support, but it requires adequate gastrointestinal function. Mare milk replacers or partially skimmed (2% fat) cow milk supplemented with 20 g/l dextrose can be used when mare milk is unavailable. Foals intolerant of enteral feeding should receive parenteral feeding in the form of a dextrose-amino acid or dextrose-lipid-amino acid mixture. Authors’ addresses: Marion Scott DuPont Equine Medical Center (McKenzie); and Middleburg Agricultural Research and Extension Center, Virginia Tech, VA (Geor); e-mail: hmckenzi@vt.edu. © 2007 AAEP.