Recombinant gene expression : reviews and protocols

Part I. General Issues About Recombinant Gene Expression Host Cell Compatibility in Protein Expression James J. Greene Production of Recombinant Proteins: Challenges and Solutions Laura A. Palomares, Sandino Estrada-Mondaca, and Octavio T. Ramirez Folding-Promoting Agents in Recombinant Protein Production Beatrix Fahnert Part II. Prokaryotes Back to Basics: pBR322 and Protein Expression Systems in E. coli Paulina Balbas and Francisco Bolivar a-Complementation Enabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experiments Mikhail F. Alexeyev, Viktoriya V. Pastukh, Inna N. Shokolenko, and Glenn L. Wilson Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli Jean-Claude Boulain and Frederic Ducancel Overexpression of Chromosomal Genes in Escherichia coli Fernando Valle and Noemi Flores Chromosomal Expression of Foreign and Native Genes From Regulatable Promoters in Escherichia coli Lu Zhou, Ke Zhang, and Barry L. Wanner Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli Sylvie Le Borgne, Francisco Bolivar, and Guillermo Gosset Copy-Control pBAC/oriV Vectors for Genomic Cloning Jadwiga Wild and Waclaw Szybalski Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV Jadwiga Wild and Waclaw Szybalski Cell-Free Protein Synthesis With Prokaryotic Combined Transcription-Translation James R. Swartz, Michael C. Jewett, and Kim A. Woodrow Genetic Tools for the Manipulation of Moderately Halophilic Bacteria of the Family Halomonadaceae Carmen Vargas and Joaquin J. Nieto Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria Amalia S. Afendra, Carmen Vargas, JoaquinJ. Nieto, and Constantin Drainas Recombinant Protein Production in Antarctic Gram-Negative Bacteria Angela Duilio, Maria Luisa Tutino, and Gennaro Marino Part III. Fungi Recombinant Protein Production in Yeasts Danilo Porro and Diethard Mattanovich Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae Kevin J. Verstrepen and Johan M. Thevelein High-Throughput Expression in Microplate Format in Saccharomyces cerevisiae Caterina Holz and Christine Lang High-Throughput Expression in Microplate Format in Pichia pastoris Mewes Bottner and Christine Lang Multiple Gene Expression by Chromosomal Integration and CRE-loxP-Mediated Marker Recycling in Saccharomyces cerevisiae Bjorn Johansson and Barbel Hahn-Hagerdal Three Decades of Fungal Transformation: Key Concepts and Applications Vianey Olmedo-Monfil, Carlos Cortes-Penagos, and Alfredo Herrera-Estrella Three Decades of Fungal Transformation: Novel Technologies Sergio Casas-Flores, Teresa Rosales-Saavedra, and Alfredo Herrera-Estrella Part IV. Plants and Plant Cells Gene Transfer and Expression in Plants Argelia Lorence and Robert Verpoorte Production of Recombinant Proteins by Hairy Roots Cultured in Plastic Sleeve Bioreactors Fabricio Medina-Bolivar and Carole Cramer Engineering the Chloroplast Genome for Hyperexpression of Human Therapeutic Proteins and Vaccine Antigens Shashi Kumar and Henry Daniell New Selection Marker for Plant Transformation Barbara Leyman, Nelson Avonce, Matthew Ramon, Patrick Van Dijck, Johan M. Thevelein, and Gabriel Iturriaga Enhancer Detection and Gene Trapping as Tools for Functional Genomics in Plants Gerardo Acosta-Garcia, Daphne Autran, and Jean-Philippe Vielle-Calzada Part V. Animals and