Miracle-dz/UniFAR-FTUs huggingface dataset:
Re-estructuración del Género Gonospermum Less. (Asteraceae: Anthemideae) en las Islas Canarias

espanolBasado en los resultados de los estudios citogeneticos llevados a cabo en los nueve taxones endemicos del complejo Gonospermum Less, Lugoa DC y Tanacetum L. en las Islas Canarias, se propone la inclusion de todos los taxones en el genero endemico Gonospermum y la re-estructuracion del mismo admitiendo la presencia de flores radiales liguladas, Gonospermum sensu Sch. Bip. , y de receptaculo desnudo sin paleas que permite incluir los taxones endemicos de Tanacetum. Se propone, por tanto, la restitucion de G. revolutum (Christen Smith in L.v. Buch) Shultz Bipontinus y las siguientes nuevas combinaciones: G. ferulaceum (Webb in Webb & Berthelot) Febles comb. nov., G. ferulaceum var. latipinnum (Sventenius) Febles comb. nov., G. ptarmicaeflorum (Webb in Webb & Berthelot) Febles comb. nov. y G. oshanahanii (Marrero Rodr., Febles & Suarez) Febles comb.nov EnglishBased on the results of cytogenetic studies carried out on the nine endemic taxa of the Gonospermum Less. , Lugoa DC. and Tanacetum L. in the Canary Islands, the inclusion of all taxa studied in the endemic genus Gonospermum is proposed with the restructuring of the genus to include the presence of ligulate flowers i.e. Gonospermum sensu Sch. Bip. and receptacle epaleate which permits the inclusion of the endemic taxa previously considered as members of the genus Tanacetum. The restitution of G. revolutum (Christen Smith in L.v. Buch) Schultz Bipontinus is proposed along with the following new combinations: G. ferulaceum (Webb in Webb & Berthelot) Febles comb. nov., G. ferulaceum var. latipinnum (Sventenius) Febles comb. nov., G. ptarmicaeflorum (Webb in Webb & Berthelot) Febles comb. nov. and G. oshanahanii (Marrero Rodr., Febles & Suarez) Febles comb.nov
Screening for Cold-Active Protease-Producing Bacteria from the Culture Collection of Polar Microorganisms and Characterization of Proteolytic Activities

The Korea Polar Research Institute (KOPRI) has assembled a culture collection of cold-adapted bacterial strains from both the Arctic and Antarctic. To identify excellent protease-producers among the proteolytic bacterial collection (874 strains), 78 strains were selected in advance according to their relative activities and were subsequently re-examined for their extracellular protease activity on ZoBell plates supplemented with 1% skim milk at various temperatures. This rapid and direct screening method permitted the selection of a small group of 15 cold-adapted bacterial strains, belonging to either the genus Pseudoalteromonas (13 strains) or Flavobacterium (2 strains), that showed proteolytic activities at temperatures ranging between . The cold-active proteases from these strains were classified into four categories (serine protease, aspartic protease, cysteine protease, and metalloprotease) according to the extent of enzymatic inhibition by a class-specific protease inhibitor. Since highly active and/or cold-adapted proteases have the potential for industrial or commercial enzyme development, the protease-producing bacteria selected in this work will be studied as a valuable natural source of new proteases. Our results also highlight the relevance of the Antarctic for the isolation of protease-producing bacteria active at low temperatures.
Fear and aggression in large flocks of laying hens

The role of the male in wild flocks of fowl, is to supervise the flock and guard it against predators, probably thereby lowering aggression and fearfulness among the members of the flock. The aim of this thesis was to study the influence of males on female behaviour in commercial farm situations by comparing all-female to sex-mixed groups of laying hens in large flocks. It was shown that males had a reducing effect on female aggression in large of laying hens (Gallus gallus domesticus) housed in groups of approx. 500 birds at a sex ratio of 1male: 24 females in a high density aviary system. However, no effect on aggression was found in flocks (ranging in size from 250 to 5000 birds) in two different loose housing systems on 25 commercial farms, probably due to the low number of males in the flocks (~ 1:350). Experiments showed that female birds in mixed flocks roosting closely together were less aggressive towards each other than birds roosting far apart. This indicates recognition of roosting partners as well as an effect of the males. Irrespective of sex composition, females in large groups were highly constant in their use of space if they were marked roosting in the ends of the compartments. Also fear reactions in females were reduced by the presence of males, as studied in large groups of 1200 birds with a sex ratio of 1:100. The vigilance behaviour and duration of tonic immobility were used as indicators, as these behaviours are considered to be protective behaviours in relation to predators. In a study of groups of 1750 birds at a sex ratio of 1:190, a positive correlation between male rank order and area covered was found. Most males, though, used more than half of the available area. However no female attachment to specific males was observed. In conclusion, males reduce female aggression and fearfulness also in large flocks and their influence on aggression is probably mainly through direct social dominance. Further, for subgroup formation in large flocks to occur, environmental features that facilitate localisation may be important.
Origin of granules in granular cell tumor. Intracellular myelin formation with autodigestion.

: Eight cases of granular cell tumor were stained with Luxol fast blue to look for the presence of myelin. Luxol fast blue-positive granules were present in all cases. One case was studied ultrastructurally. It showed evidence of origin of granules as infoldings of cell membrane by a process similar to myelin formation around axons. These infoldings subsequently appear to be phagocytosed by lysosomes, resulting in the typical granules of the granular cell tumor.
Effect of crestar administration on oestrus response in postpartum anoestrous cows

The oestrus responses following Crestar administration with gonadotrophin in postpartum anoestrous cows was studied. In the treatment group, 9 out of 10 postpartum anoestrous cows exhibited oestrus between 22.00 to 37.50 hr after removal of Crestar ear implant. The control cows exhibited natural oestrus between 66 to 160 days of placebo treatment. The mcan duration of oestrus was 19.11 ± 0.34 and 15.40 ± 2.15 hr in the treatment and control groups. respectively. with no significant difierence. All cows of both the groups ovulated (100%) following onset of ocstrus. In the treatment group. out of 9 cows induceed to oestrus 33.33, 55.56 and 11.11 % showed intense, moderate and weak symptom of oestrus rcspectively. Tile corresponding percentage in the control group were 20, 40 and 40 respectively. Status of the genital tract at hormonally induced oestrus was similar with naturnl oestrus in postpartum indigenous cows.
Non-Invasive Assessment of Liver Fibrosis in a Rat Model: Comparison of Shear Wave Elastography with Serologic Tests

Background: Non-invasive evaluation of hepatic fibrosis, especially serum biomarkers and ultrasound elastography, has vigorously developed during the past decades. Objectives: The purpose of this study was to evaluate the diagnostic performance of the latest generation of ultrasound elastographytwo-dimensional real-time shear wave elastography (SWE) in accessing liver fibrosis progression in comparison with serologic tests in a rat model. Materials and Methods: Liver fibrosis was induced in 108 rats (control group: 18 rats, experiment group: 90 rats) by intraperitoneal injection of thioacetamide (300mg/kg, twice a week for 12 weeks). SWE examination was done by two operators. Hyaluronic acid (HA), aspartate aminotransferase (AST) to platelet (PLT) ratio index (APRI), AST to alanine aminotransferase (ALT) ratio (AAR) were used for serology tests. Histological fibrosis stages (F0-F4) according to the Scheuer scoring system were referred as the gold standard. Diagnostic performance of elastography and serology methods were determined by the areas under receiver operating characteristic curve (AUROC). Results: Liver stiffness values increased with the progression of hepatic fibrosis. Liver stiffness assessed by SWE exhibited a strong positive correlation with histological fibrosis stages (r = 0.849, P < 0.001). SWE showed a better diagnostic performance than the selected serological biomarkers HA, APRI, and AAR (AUROC for SWE, HA, APRI, and AAR were 0.933, 0.707, 0.714, and 0.691 in diagnosing F ≥ F2 and 0.986, 0.807, 0.870, and 0.770 in diagnosing F = F4, respectively). Conclusion: Ultrasound elastography SWE technique is a reliable method for noninvasive evaluation of liver fibrosis in comparison with the serological biomarkers, HA, APRI, and AAR.
Estimação dos coeficientes de digestibilidade ileal aparente das proteínas e aminoácidos do torta de canola em leitões recém-desmamados

ResumenPara obtener los coeficientes de digestibilidad ileal aparente (CDIA) y estandarizada (CDIE) de la proteina y aminoacidos de pasta de canola (PC), se realizo un experimento en el que se utilizaron 24 lechones destetados a los 17 dias de edad con un peso de 5.5 kg, a los cuales se les fijo una canula simple en “T” al final del ileon distal. Los lechones recibieron uno de los tratamientos: Dieta de Referencia (DR) elaborada con caseina como unica fuente de proteina y almidon de maiz, dietas CTC (caseina 10% de torta de canola) y CTCP (caseina- 10% de torta de canola peletizada). Los resultados mostraron que los CDIA de la DR fueron superiores (p<0.05) a los de la dieta CTCP y estos a los de la dieta CTC. Los CDIE fueron superiores en torta de canola paletizada (TCP) e inferiores en torta de canola (TC). Se concluye que la TCP puede utilizarse a un nivel de inclusion maximo del 10% en el alimento de lechones recien destetados, ya que el peletizado mejoro significativamente la digestibilidad ileal de proteina y aminoacidos.
Controle gentico da exigncia trmica para o incio do florescimento em milho

The objective of this work was to study the genetic control of thermal demand for maize flowering initiation. Two contrasting lines were crossed and used with the F1, F2 RC1 and RC2 derived populations. The trait day-degrees between sown and flowering per plant were evaluated to obtain the estimates of components of variance and means. All models of mean components had R2 values near 100%, and then the simple additive-dominant model was considered. Considering the components of variance the model with additive, dominant and environment variance explained the genetic control of maize flowering. The results of the estimated components allowed to conclude that genetic control of earlyness in maize is oligogenic with presence of dominance. Moreover, high values of estimate heritability coefficient indicate the possibility of success with individual phenotypic selection.
Oestrus induction and conception in buffaloes after hormonal treatment during summer

Experiment was conducted on 24 Murrah buffaloes during summer maintained at livestock production research farm of the institule. All the buffaloes were in anoestrus condition, but some had small sized corpus luteum. whereas others possessed smooth ovaries after rectal palpation on 3 successive occasions. Buffaloes (10) which had corpus luteum, were given 25 mg first PG injection i/m (5.0 ml lutalyse), whereas buffaloes received 5.0 ml normal saline solution to serve as control. Buffaloes were detected in oestrus by teaser bull twice a day. Animals not observed in oestrus after first PG. were further given 5.0 ml lutalyse at an interval of 10 days. Blood was collected from each buffaloes at the time of PG injection as well as at oestrus for estimation of progesterone. in plasma. In another group, remaining 8 buffaloes with smooth ovaries were treated with progesterone and oestradiol regimen. Buffaloes (5 and 3) expressed oestrus activity after first and second PG treatment respectively. Out of 5 buffaloes in first PG group, only 2 expressed standing oestrus symptom and remaining 3 had uterine tone. No buffalo was observed in standing oestrus in second PG treated group. Only 2 buffaloes become pregnant and that to in first PG treated group. Conception was nil in second PG and in control group. Progesterone estimation revealed that PG was effective only in those buffaloes, which had more than 1.0 ng/ml blood progesterone concentration at the time of treatment. Progesterone concentration was lower then 1.0 ng/ml of blood in all control and non-pregnant treated buffaloes. In other experiment, oestrus was induced in 75% (6/8) buffaloes, when progesterone and oestradiol was injected. Four buffaloes were observed in standing oestrus and 2 expressed mucus discharge after 48 hr to treatment. Conception rate was 50% (418) in this treatment group. Findings of above experiment suggest that supplementation of exogenous progesterone is essential for oestrus induction and pregnancy in acyclic buffaloes during summer.
Valoración del cumplimiento de los tratamientos hipoglucemiantes y antihipertensivos en Galicia (‘CumpleGa’)

Objective: To evaluate the prevalence of therapeutic noncompliance in diabetes and arterial hypertension in Galician community pharmacies (FC), identifying the factors associated with patients’ non-adherence and perception of their treatment. Methods: A cross-sectional, descriptive, randomized study conducted in August and September 2016 among adult patients receiving treatment with hypoglycemic and/or antihypertensive drugs for at least two months with the same treatment regimen. Noncompliance was assessed with the administration of three questionnaires: Batalla, Haynes-Sacket y Morisky-Green, in one page of data collection that included the three questionnaires, as well as the demographic data of each patient. Results: 31 community pharmacies participated in 27 pharmacies and collected 1,588 (sample size: 1,537) valid questionnaires: 778 (49.0%) females and 810 (51.0%) males, aged 67.6±11.1 years on average. The number of non-compliant patients at least in one of the three tests was 1,245 (78.4%). The average noncompliance was 42.6%. Treatment perception was negative for 35.6% of patients. 51.0% of patients ignore the complications of their illness, 33.1% forget to take their medication, and 29.5% do not believe their prescribed medications are suitable. Conclusions: Noncompliance with the therapeutic guidelines for diabetic and hypertension medication in Galicia is high and affects nearly half the patient population. Poor knowledge of the disease, lower level of education, multiple medication, pensioner age (elderly) and negative perception of medications seem to correlate with a higher prevalence of treatment noncompliance.
Molecular and Functional Characterization of Murine Bestrophin 1 Cloned from Heart

Calcium‐activated chloride channels (ClCa) are widely distributed in cardiac tissues and play important roles in the regulation of cardiac excitability. However, the molecular identity of this channel remains unknown. Bestrophins were recently shown to form ClCa when expressed heterologously. We hypothesize that Bestrophins are candidates for the calcium‐activated chloride current (IClCa) in cardiac tissues. We demonstrate that several members of this family are expressed in heart. Previous studies have reported the functional expression of two members of the murine Bestrophin family, mBest2 and mBest3. Here we report the functional characterization of mBest1. We isolated the cDNA transcript of mBest1 from mouse heart and cloned it into a mammalian expression vector so that functional analysis could be performed to determine if it encodes a ClCa. Whole‐cell patch clamp experiments with HEK cells transfected with mBest1 revealed a calcium sensitive, time independent chloride current. This DIDS‐ and niflumic acid‐sensitive current displayed slight voltage dependence and exhibited a lyotropic sequence of anion permeability, SCN−>I−>Cl−. Our data contributes to the characterization of the biophysical properties of Bestrophins and helps further the investigation into the molecular candidate of cardiac IClCa.
Creating awareness of rabies in pupil of Z.P. High School in Kallur (V), Kurnool (Dist), Andhra Pradesh

Rabies is a fatal but easily preventable disease. It has worldwide distribution, Humans and nearly all mammals are susceptible to rabies. In spite of presence of pre and post exposure prophylaxis, it is still a significant cause of public health problem in India. The World Health Organization (WHO) supports targets for elimination of human rabies transmitted by dogs in South-East Asia by 2020. In India, animal bites in humans are a public health problem and an estimated 17.4 million animal bites occur annually. Since 1985, India has reported an estimated 25,000–30,000 human deaths from rabies annually. The majority of people who die of rabies are people of poor or low-income socioeconomic status. Most animal bites in India (91.5%) are by dogs, of which about 60% are strays and 40% pets. The incidence of animal bites is 17.4 per 1000 population. A person is bitten every 2 seconds, and someone dies from rabies every 30 minutes. The annual number of person-days lost because of animal bites is 38 million, and the cost of post bite treatment is about $25 million. Dog bites are ABSTRACT
Comparison of traditional and genomic breeding programs for organic and low input dairy cattle accounting for traits relevant in different macro-climatic zones

In the past decade, successful selection on production traits for dairy cattle has greatly increased milk production. Recently, selection indices for female fertility were gradually and increasingly introduced into the overall breeding goals for dairy cattle (Miglior et al., 2005). As a by-product of fermention in ruminants, enteric methane emissions (ME) should also be controlled and mitigated due to their contribution to global warming (Forster et al., 2007) and as a cause for inefficient use of dietary energy. Moderate heritabilities ranging between 0.30 and 0.35 for predicted and real measurements of ME were reported for dairy cows and ewe lambs (de Haas et al., 2011; Pinares-Patino et al., 2011), indicating that a heritable component for ME is available for implementing sustainable breeding strategies to reduce ME in dairy farms. In dairy cattle production systems, the traditional progeny testing substantially increases accuracy of selection especially for bulls. However, availability of high-density SNP arrays enable dairy cattle breeders to apply genomic selection in their breeding strategies. Consequently, the objective of this study was to compare selection response for a complex breeding goal comprising ME, milk yield (MY), days open (DO), clinical mastitis (CM), body condition score (BCS) and milking temperament (MT) and total discounted return for organic and low input dairy cattle (with organic Brown Swiss as an example) from progeny testing and genomic breeding program by applying ZPLAN+ (Taubert et al., 2010).
Plk1 regulates PICH, a centromere-associated SNF2 family tranlocase that is required for the spindle checkpoint

To investigate the requirements of mitotic, kinetochore-associated proteins for human chromosome segregation we analyzed the human Ndc80 complex, a core structural component of the outer kinetochore. The Ndc80 complex contains Hec1 and Nuf2 that interact via their N-termini and a smaller subcomplex of Spc24 and Spc25 is linked to Hec1. The complex is required for faithful chromosome congression and the recruitment of several proteins of the outer kinetochore, including the mitotic regulatory kinase Plk1. Pull down experiments with Plk1 identified a novel kinetochore/centromere associated DNA translocase, which we termed PICH, as an interactor of Plk1. The localization of PICH to kinetochores and centromeres is controlled by Plk1; and moreover, Plk1 phosphorylation on PICH negatively regulates its localization / chromatin association. PICH associates with conspicuous threads that persist into anaphase where Topoisomerase II causes their resolution. Moreover, PICH is a novel component of the spindle assembly checkpoint and PICH-dependent checkpoint signaling is likely to be mediated via kinetochore associated Mad2. This study raises questions as to the fate of centromeric DNA in sister chromatid separation and its timing of decatenation. We speculate that this enzyme associates with catenated, centromeric DNA from prometaphase where it may be required to sense the tension between sister kinetochores.
Effect of protein phosphorylation inhibitor on production of parthenogenetic caprine embryos

The objective of the present study was to compare the effect of different concentrations of 6-Dimethylaminopurine (6-DMAP) treatment in chemical activated oocytes to compare the developmental potency of parthenogenetic caprine embryos. Morphologicaly matured oocytes were denuded and activated with 5 μm calcium ionophore (ionomycin) for 5 min. Activated oocytes were randomly divided into 5 groups: Group 1 oocytes were cultured in modified Charles Rosenkrans medium (mCR2aa), without DMAP treatment, group 2 cultured in mCR2aa with 2.5 mM DMAP, group 3 cultured in mCR2aa with 5 mM DMAP, group 4 cultured in mCR2aa with 10 mM DMAP, group 5 cultured in mCR2aa with 20 mM DMAP for 4 h. The presumptive zygotes of 5 groups were washed and cultured in the embryo development medium. Development of parthenotes was observed at every 48 h till day 12 post insemination. The percentage of cleavage, morula, blastocyst and hatched blastocyst production in groups 1, 2, 3, 4 and 5 were 61.76, 6.67, 0.00 and 0.00; 59.79, 27.43, 10.62 and 0.00; 72.43, 41.62, 10.66 and 1.52; 64.61, 50.44, 7.83 and 0.00; 63.19, 19.42, 3.88 and 0.00% respectively. Results indicated that 5 mM DMAP treatment for chemically activated oocyte is the best treatment for development of parthenogenetic caprine embryos. In conclusion, it can be stated that for the production of caprine parthenogenetic embryos in vitro, concentration of protein phosphorylation inhibitor plays an important role in the activation treatment. Optimized activation protocols could enhance the production of viable parthenogenetic embryos.
Use of Modified CHA2DS2-VASC Score for Risk Prediction in Patients Undergoing PCI

Abstract Background: Most of the existing risk prediction models include angiographic or procedural variables thus making it difficult for their application in decision making at the outset of treatment. This study we used modified CHA2DS2-VASC score as a simple tool for risk stratification of patients with PCI, regardless of atrial fibrillation (AF). Methods: This is a prospective observation study of patients who had undergone PCI, with diagnosis of either ACS or chronic stable angina between the age group of 18 to 85 years. Modified CHA2DS2-VASC score (CAD presence was not given one point) was calculated in the patients before PCI and followed for occurrence of MACCE or death. Score was correlated with primary outcome death or MACCE. Results: Total of 676 patients were considered for this study, Mean modified CHA2DS2-VASC score of the study population is 1.86± 1.26. Sub group analysis of the patients by dividing into groups with a score less than 1 and greater than 1 revealed that there is no statistical difference in either MACCE ( p = 0.31) or death (p=0.6) between the groups. Subgroup analysis of ≤1 and ≥5 modified CHA2DS2VASc score has no statistically significant difference in MACCE (p=0.45), but has statistically significant difference in death rate by Fischer exact test(p=0.02) and by chi-square Yates correction (p=0.01). Further analysis also shows that patients with score ≥5 had 13 times increased risk of death when compared with score ≤1. Conclusion: Modified CHA2DS2-VASc Score is a simple clinical tool for predicting outcome in patients planned for PCI. Risk of death clearly correlate with the score when score was more than 5 point.