Miracle-dz/UniFAR-FTUs huggingface dataset:
A Vibrational Technique for In Vitro Intraoperative Prosthesis Fixation Monitoring

Objective: In this paper, a new vibrational modal analysis technique was developed for intraoperative cementless prosthesis fixation evaluation upon hammering. Methods: An artificial bone (Sawbones)-prosthesis system was excited by sweeping of a sine signal over a wide frequency range. The exponential sine sweep technique was implemented to the response signal in order to determine the linear impulse response. Recursive Fourier transform enhancement (RFTE) technique was applied to the linear impulse response signal in order to enhance the frequency spectrum with sharp and distinguishable peak values indicating distinct high natural frequencies of the system (ranging from 15 kHz to 90 kHz). The experiment was repeated with 5 Sawbones-prosthesis samples. Upon successive hammering during the prosthesis insertion, variation of each natural frequency was traced. Results: Compared to classical Fast Fourier Transform, RFTE provided a better tracing and enhancement of frequency components during insertion. Three different types of frequency evolving trends (monotonically increasing, insensitive, and plateau-like) were observed for all samples, as confirmed by a new finite element simulation of the prosthesis dynamic insertion. Two main mechanical phenomena (i.e., geometrical compaction and compressive stress) were shown to govern these trends in opposite ways. Follow-up of the plateau-like trend upon hammering showed that the frequency shift is a good indicator of fixation. Conclusion: Alongside the individual follow-up of frequency shifts, combinatorial frequency analysis provides new objective information on the mechanical stability of Sawbone-prosthesis fixation. Significance: The proposed vibrational technique based on RTFE can provide the surgeon with a new assistive diagnostic technique during the surgery by indicating when the bone-prosthesis fixation is acceptable, and beyond of which further hammering should be done cautiously to avoid bone fracture.
Controlled release of bFGF loaded into electrospun core–shell fibrous membranes for use in guided tissue regeneration

In this study, basic fibroblast growth factor (bFGF) was loaded into a poly(lactic-co-glycolic acid) (PLGA)/wool keratin composite membrane by emulsion electrospinning to prepare a composite membrane with a core–shell structure for guided tissue regeneration (GTR). The physicochemical properties, drug release performance in vitro and cytotoxicity of the composite membrane were evaluated to select the optimum concentration of bFGF. The fibers in the six groups of composite membranes were uniform in thickness, had a smooth surface, and did not exhibit a string bead structure. In addition, the fibers in the six groups of composite membranes had a stable core–shell structure, in which the core layer of the fibers was wrapped around bFGF and the shell layer of the fibers comprised the PLGA/wool keratin oil-phase component. Compared with the PLGA/wool keratin composite membrane prepared by traditional electrospinning, the composite membranes prepared by emulsion electrospinning had a higher water absorption rate and superior hydrophilicity. The bFGF encapsulation rate of the 10 μg bFGF composite membrane was the highest (97.3% ± 11.3%). Stable and sustained release of bFGF from the five groups of bFGF-loaded PLGA/wool keratin composite membranes can be maintained over 28 d. The five groups of PLGA/wool keratin composite membranes loaded with bFGF could promote the adhesion, proliferation and osteogenic differentiation of human periodontal ligament fibroblasts (hPLDFs) to varying degrees. Among the five groups, the cell morphology, proliferation ability and osteogenic differentiation ability of the 10 μg bFGF composite membrane group were the best. This study will serve as a foundation for further research on bFGF-loaded composite membranes for GTR applications.
Non‐GMO potato lines, synthesizing increased amylose and resistant starch, are mainly deficient in isoamylase debranching enzyme

Summary Solanum tuberosum potato lines with high amylose content were generated by crossing with the wild potato species Solanum sandemanii followed by repeated backcrossing to Solanum tuberosum lines. The trait, termed increased amylose (IAm), was recessive and present after three generations of backcrossing into S. tuberosum lines (6.25% S. sandemanii genes). The tubers of these lines were small, elongated and irregular with small and misshaped starch granules and high sugar content. Additional backcrossing resulted in less irregular tuber morphology, increased starch content (4.3%–9.5%) and increased amylose content (29%–37.9%) but indifferent sugar content. The amylose in the IAm starch granules was mainly located in peripheral spots, and large cavities were found in the granules. Starch pasting was suppressed, and the digestion‐resistant starch (RS) content was increased. Comprehensive microarray polymer profiling (CoMPP) analysis revealed specific alterations of major pectic and glycoprotein cell wall components. This complex phenotype led us to search for candidate IAm genes exploiting its recessive trait. Hence, we sequenced genomic DNA of a pool of IAm lines, identified SNPs genome wide against the draft genome sequence of potato and searched for regions of decreased heterozygosity. Three regions, located on chromosomes 3, 7 and 10, respectively, displayed markedly less heterozygosity than average. The only credible starch metabolism‐related gene found in these regions encoded the isoamylase‐type debranching enzyme Stisa1. Decreased expression of mRNA (>500 fold) and reduced enzyme activity (virtually absent from IAm lines) supported Stisa1 as a candidate gene for IAm.
Current WHO hemoglobin thresholds for altitude and misdiagnosis of anemia among Tibetan highlanders

This may result in lower hematological indices on admission as those patients were already in critical condition. Further studies should be conducted comparing patientsʼ onset of symptoms and correlating their clinical condition to laboratory findings. In conclusion, our study showed that on admission, older age, lymphopenia and raised LDH were associated with ICU admissions. Patients who were transferred to the ICU had a deeper nadir ALC, nadir AMC and nadir hemoglobin, and higher peak ANC and peak LDH levels as compared to patients who did not require ICU stay.
GKOLIAS IOANNIS CHAOS IN 1-DIMENSIONAL NONLINEAR DISORDERED LATTICES

The interplay between nonlinearity and disorder has been the subject of many recent studies. Due to Anderson localization, the normal modes of a 1dimensional disordered lattice of linear oscillators are exponentially localized. When nonlinearities are also added to the model a natural question arises: Will the chaotic processes, induced by the nonlinear terms of the equations, enhance the localization or they will destroy it? The latest numerical results confirm a subdiffusive spreading of an initially localized wavepacket. The purpose of this thesis is to try to lighten the dynamics behind the diffusive behaviour. Using numerical techniques, like symplectic integrators and their extension to the tangent map method, not only we qualitatively observe the spreading process, but a quantitative approach is used with the aid of chaos detection indicators. For the first time systematic numerical calculations of finite Lyapunov exponents are reported for such systems. Our findings yield that the chaotic time-scale of the system is shorter than the spreading time scales, supporting the theories that the diffusion is a process driven by chaos.
Characteristics of Microsatellites Mined from Transcriptome Data and the Development of Novel Markers in Paeonia lactiflora

The insufficient number of available simple sequence repeats (SSRs) inhibits genetic research on and molecular breeding of Paeonia lactiflora, a flowering crop with great economic value. The objective of this study was to develop SSRs for P. lactiflora with Illumina RNA sequencing and assess the role of SSRs in gene regulation. The results showed that dinucleotides with AG/CT repeats were the most abundant type of repeat motif in P. lactiflora and were preferentially distributed in untranslated regions. Significant differences in SSR size were observed among motif types and locations. A large number of unigenes containing SSRs participated in catalytic activity, metabolic processes and cellular processes, and 28.16% of all transcription factors and 21.74% of hub genes for inflorescence stem straightness were found to contain SSRs. Successful amplification was achieved with 89.05% of 960 pairs of SSR primers, 55.83% of which were polymorphic, and most of the 46 tested primers had a high level of transferability to the genus Paeonia. Principal component and cluster dendrogram analyses produced results consistent with known genealogical relationships. This study provides a set of SSRs with abundant information for future accession identification, marker-trait association and molecular assisted breeding in P. lactiflora.
A Novel One‐Day Learning Procedure for Mice

Current preclinical cognitive assessments are highly time intensive, with lengthy assessment procedures. In this regard, a single‐day assay that focuses just on assessing learning behavior in a time‐effective and relatable manner would be of value. This article describes the box maze as a short‐term behavioral procedure to measure learning in mice. The protocol consists of allowing mice to explore an enclosed space that has eight holes. One of these holes leads to a tunnel that connects to an escape cage, and the latency to enter this escape hole is recorded for each mouse. Mice are tested four times within a single day, and the decrease in escape latency over time is used as a measure of learning. Age is a factor that affects escape latency in the box maze. Hence, the box‐maze procedure is proposed as an efficient test to probe aging and aging intervention–related research questions. © 2020 by John Wiley & Sons, Inc.
Species‐specific secretion of ESX‐5 type VII substrates is determined by the linker 2 of EccC5

Mycobacteria use type VII secretion systems (T7SSs) to translocate a wide range of proteins across their diderm cell envelope. These systems, also called ESX systems, are crucial for the viability and/or virulence of mycobacterial pathogens, including Mycobacterium tuberculosis and the fish pathogen Mycobacterium marinum. We have previously shown that the M. tuberculosis ESX‐5 system is unable to fully complement secretion in an M. marinum esx‐5 mutant, suggesting species specificity in secretion. In this study, we elaborated on this observation and established that the membrane ATPase EccC5, possessing four (putative) nucleotide‐binding domains (NBDs), is responsible for this. By creating M. marinum‐M. tuberculosis EccC5 chimeras, we observed both in M. marinum and in M. tuberculosis that secretion specificity of PE_PGRS proteins depends on the presence of the cognate linker 2 domain of EccC5. This region connects NBD1 and NBD2 of EccC5 and is responsible for keeping NBD1 in an inhibited state. Notably, the ESX‐5 substrate EsxN, predicted to bind to NBD3 on EccC5, showed a distinct secretion profile. These results indicate that linker 2 is involved in species‐specific substrate recognition and might therefore be an additional substrate recognition site of EccC5.
In vitro biocompatibility of biohybrid polymers membrane evaluated in human gingival fibroblasts

Abstract The biohybrid polymer membrane (BHM) is a new biomaterial designed for the treatment of soft periodontal tissue defects. We aimed to evaluate the in vitro biocompatibility of the membrane in human gingival fibroblasts and the capability to induce cell adhesion, migration, differentiation and improving the production of the extracellular matrix. BHM and Mucograft® collagen matrix (MCM) membranes were punched into 6 mm diameter round discs and placed in 96‐well plates. Human primary gingival fibroblasts were seeded on the membranes or tissue culture plastic (TCP) serving as the control. Cell proliferation/viability and morphology were evaluated after 3, 7, and 14 days of culture by cell counting kit (CCK)‐8 assay and scanning electron microscopy, respectively. Additionally, the gene expression of transforming growth factor (TGF)‐β1, focal adhesion kinase (FAK), collagen type 1 (Col1), alpha‐smooth muscle actin (α‐SMA), and fibroblasts growth factor (FGF)‐2 was analyzed at 3, 7, and 14 days of culture by qPCR. Cell proliferation on BHM was significantly higher than on MCM and similar to TCP. Gene expression of TGF‐β1, FAK, Col1, and α‐SMA were significantly increased on BHM compared to TCP at most investigated time points. However, the gene expression of FGF‐2 was significantly decreased on BHM at Day 7 and recovered at Day 14 to the levels similar to TCP. The finding of this study showed that BHM is superior for gingival fibroblasts in terms of adhesion, proliferation, and gene expression, suggesting that this membrane may promote the healing of soft periodontal tissue.
3D Skeletal Gesture Recognition via Hidden States Exploration

Temporal dynamics is an open issue for modeling human body gestures. A solution is resorting to the generative models, such as the hidden Markov model (HMM). Nevertheless, most of the work assumes fixed anchors for each hidden state, which make it hard to describe the explicit temporal structure of gestures. Based on the observation that a gesture is a time series with distinctly defined phases, we propose a new formulation to build temporal compositions of gestures by the low-rank matrix decomposition. The only assumption is that the gesture’s “hold” phases with static poses are linearly correlated among each other. As such, a gesture sequence could be segmented into temporal states with semantically meaningful and discriminative concepts. Furthermore, different to traditional HMMs which tend to use specific distance metric for clustering and ignore the temporal contextual information when estimating the emission probability, we utilize the long short-term memory to learn probability distributions over states of HMM. The proposed method is validated on multiple challenging datasets. Experiments demonstrate that our approach can effectively work on a wide range of gestures, and achieve state-of-the-art performance.
Spatial Filtering in SSVEP-Based BCIs: Unified Framework and New Improvements

Objective: In the steady-state visual evoked potential (SSVEP)-based brain computer interfaces (BCIs), spatial filtering, which combines the multi-channel electroencephalography (EEG) signals in order to reduce the non-SSVEP-related component and thus enhance the signal-to-noise ratio (SNR), plays an important role in target recognition. Recently, various spatial filtering algorithms have been developed employing different prior knowledge and characteristics of SSVEPs, however how these algorithms interconnect and differ is not yet fully explored, leading to difficulties in further understanding, utilizing and improving them. Methods: We propose a unified framework under which the spatial filtering algorithms can be formulated as generalized eigenvalue problems (GEPs) with four different elements: data, temporal filter, orthogonal projection and spatial filter. Based on the framework, we design new spatial filtering algorithms for improvements through the choice of different elements. Results: The similarities, differences and relationships among nineteen mainstream spatial filtering algorithms are revealed under the proposed framework. Particularly, it is found that they originate from the canonical correlation analysis (CCA), principal component analysis (PCA), and multi-set CCA, respectively. Furthermore, three new spatial filtering algorithms are developed with enhanced performance validated on two public SSVEP datasets with 45 subjects. Conclusion: The proposed framework provides insights into the underlying relationships among different spatial filtering algorithms and helps the design of new spatial filtering algorithms. Significance: This is a systematic study to explore, compare and improve the existing spatial filtering algorithms, which would be significant for further understanding and future development of high performance SSVEP-based BCIs.
Dietary Patterns of Breakfast Consumption Among Chilean University Students

Breakfast is one of the most important meals of the day. A good quality breakfast must include dairy products, cereals, and fruits. The aim of this study is to determine breakfast dietary patterns and their nutritional quality among Chilean university students. A cross-sectional non-probabilistic study was conducted in 200 university students between 18 and 27 years in Santiago, Chile. To identify dietary patterns and breakfast quality, a breakfast food survey was conducted. Patterns were identified by factor analysis. Most of the subjects (53%) ate breakfast daily, with a higher prevalence among females (60.2% vs. 43.7%, p < 0.05); 68% did not consume fruits and 17.5% had good breakfast quality, with no differences by sex. Four breakfast dietary patterns were identified: “dairy & cereals”, “healthy”, “traditional salty” and “traditional sweet” that together explained 35.6% of the total variance. There was no sex difference in predominant dietary patterns. The “dairy & cereals” and “traditional sweet” patterns were associated with regularly eating breakfast (β: −0.47, p = 0.001; β: −0.32, p = 0.020) and the “healthy” pattern with BMI ≥25 kg/m2 (β: 0.35, p = 0.024). In conclusion, breakfast quality was inadequate due to low fruit consumption and energy intake. The four identified patterns included cereals, bread, dairy, fats and sugars. Results may be usual in the planning of future interventions aimed at improving breakfast consumption and quality in university students.
Acorus gramineusand and Euodia ruticarpa Steam Distilled Essential Oils Exert Anti-Inflammatory Effects Through Decreasing Th1/Th2 and Pro-/Anti-Inflammatory Cytokine Secretion Ratios In Vitro

To clarify the effects of steam distilled essential oils (SDEO) from herbs used in traditional Chinese medicine on immune functions, two potential herbs, Acorus gramineusand (AG) and Euodia ruticarpa (ER) cultivated in Taiwan, were selected to assess their immunomodulatory effects using mouse primary splenocytes and peritoneal macrophages. T helper type 1 lymphocytes (Th1) (IL-2), Th2 (IL-5), pro-inflammatory (TNF-α) and anti-inflammatory (IL-10) cytokines secreted by correspondent immune cells treated with SDEO samples were determined using enzyme-linked immunosorbent assay. The total amounts of potential phytochemicals, including total flavonoids, polyphenols and saponins, in these two selected SDEOs were measured and correlated with cytokine levels secreted by immune cells. Our results evidenced that ER SDEO is rich in total flavonoids, polyphenols and saponins. Treatments with AG and ER SDEO significantly (p < 0.05) increased IL-5/IL-2 (Th2/Th1) cytokine secretion ratios by splenocytes, suggesting that both AG and ER SDEO have the Th2-polarization property and anti-inflammatory potential. In addition, AG and ER SDEO, particularly ER SDEO, markedly decreased TNF-α/IL-10 secretion ratios by macrophages in the absence or presence of lipopolysaccharide (LPS), exhibiting substantial effects on spontaneous and LPS-induced inflammation. Significant correlations were found between the total polyphenols, flavonoids or saponins content in the two selected SDEOs and Th1/Th2 immune balance or anti-inflammatory ability in linear, non-linear or biphasic manners, respectively. In conclusion, our results suggest that AG and ER, particularly ER, SDEO have immunomodulatory potential in shifting the Th1/Th2 balance toward Th2 polarization in splenocytes and inhibiting inflammation in macrophages in the absence or presence of LPS.
Hospice Utilization Among Residents in Long-Term Care Facilities

Background: Hospice care can improve quality of life for persons nearing end of life, yet little is known about utilization of hospice care among persons residing in long-term care facilities (LTCFs). Given the increasing number of deaths that occur in LTCFs, it is important to examine hospice care practices in LTCFs. Aim: The aim of the cross-sectional study was to describe residents who received hospice care in LTCFs and explore factors that can predict hospice use in LTCFs across Canada. This study included 185 715 residents aged 19 years or older in LTCFs in Canada in 2015. Results: Of all residents, 2.7% (n = 4973) received hospice care and 6.8% (n = 12 684) were profiled as having an end-stage disease. Among those who received hospice care, most were noted as end stage (89.5%) and had severe physical impairment (Activities of Daily Living Hierarchy Scale ≥ 5, 74.3%), mild-to-severe pain (Pain Scale ≥ 1, 76.0%), and moderate-to-severe health instability (Changes in Health, End-Stage Disease, Signs, and Symptoms Scale ≥3, 82.9%). Residents who received hospice care were in more severe and complex clinical conditions than those who did not receive hospice care. Conclusion: Only a small proportion of residents in LTCFs received hospice care. Further investigation of standardized assessment of terminal status is needed as accuracy of end-stage diagnosis continues to be challenging and criteria for hospice eligibility are narrow. Special attention should be paid to improve access to hospice care among residents with dementia or other progressive chronic diseases with severe and complex clinical needs.
The functional organization of excitation and inhibition in the dendrites of mouse direction-selective ganglion cells

Recent studies indicate that the precise timing and location of excitation and inhibition (E/I) within active dendritic trees can significantly impact neuronal function. How synaptic inputs are functionally organized at the subcellular level in intact circuits remains unclear. To address this issue, we took advantage of the retinal direction-selective ganglion cell circuit, where directionally tuned inhibition is known to shape non-directional excitatory signals. We combined two-photon calcium imaging with genetic, pharmacological, and single-cell ablation methods to examine the extent to which inhibition ‘vetoes’ excitation at the level of individual dendrites of direction-selective ganglion cells. We demonstrate that inhibition shapes direction selectivity independently within small dendritic segments (<10µm) with remarkable accuracy. The data suggest that the parallel processing schemes proposed for direction encoding could be more fine-grained than previously envisioned.
SBA-16 Cage-Like Porous Material Modified with APTES as an Adsorbent for Pb2+ Ions Removal from Aqueous Solution

Tridimensional cubic mesoporous silica, SBA-16, functionalized with aminopropyl groups, were employed as adsorbents for Pb2+ ion removal from aqueous solution. The adsorption capacity was investigated for the effect of pH, contact time, temperature, and concentration of 3-aminopropyltriethoxysilane (APTES) employed for adsorbent functionalization. The textural properties and morphology of the adsorbents were evaluated by N2 physisorption, small-angle X-ray diffraction (XRD), diffuse reflectance spectroscopy (UV-vis), and transmission electron microscopy (TEM). The functionalization of the SBA-16 was evaluated by elemental analysis (N), thermogravimetric analysis (TG), Fourier transform infrared spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS). Batch adsorption studies show that the total Pb2+ ions removal was archived on adsorbent having an optimized amount of aminopropyl groups (2N-SBA-16). The maximum of Pb2+ ions removal occurred at optimized adsorption conditions: pH = 5–6, contact time 40 min, and at a low initial lead concentration in solution (200 mg L−1). Under the same adsorption conditions, the amino-functionalized SBA-16 with cubic 3D unit cell structure exhibited higher adsorption capability than its SBA-15 counterpart with uniform mesoporous channels.