Miracle-dz/UniFAR-FTUs huggingface dataset:
Flow cytometric immunophenotyping including Bcl-2 detection on fine needle aspirates of lymph node in the diagnosis of Diffuse Large B-Cell Lymphoma

1 Associate professor, Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh 2 Bacteriologist, Institute of Public Health, Mohakhali, Dhaka-1212 3 Department of Microbiology and Immunology Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh Corresponding Author Dr Shirin Tarafder Associate Professor, Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh Email: starafder2007@yahoo.com, sarwardocument@gmail.com, Phone: +8801732 622242 Abstract Diffuse large B-cell lymphoma (DLBCL) is a fast growing and aggressive high grade non-Hodgkin lymphoma (HG-NHL) that spreads quickly, present with lymphadenopathy or enlarged nodes in mediastinum, mesenteric region or peritoneum, from where tissue excision and biopsy with histopathology is not possible. The aim of this study was to diagnose DLBCL by Flow cytometric immunophenotyping (FCI) on fine needle aspirate (FNA) of lymphnode following immunophenotypic diagnostic criteria based on expression of CD markers. All samples were preliminary assessed by fine needle aspiration cytology (FNAC) as NHL or lymph proliferative disorder (LPD). FCI was performed with a complete panel of antibodies (CD3, CD4, CD8, CD5, CD7, CD10, CD19, CD20, CD23, CD22, CD25, CD30, CD45, CD79a, CD79b, CD95, CD56, FMC7, CD40, CD15, Kappa, Lambda and Bcl-2) by dual color flowcytometry. FCI data were interpreted to diagnose and sub classify NHL according to WHO classification. Wherever possible the diagnoses were compared with available immunohistochemistry (IHC) and histopathology reports. During one year period (from February 2016 to March 2017)10 cases of DLBCL were identified by FCI. Out of 10 DLBCL cases, 8 histopathology and 6 1HC reports were available of which 6 DLBCL cases showed 100% (6/6) concordance with combined histopathology and IHC and 100% concordance with IHC alone; 75% (6/8) concordance and 25% (2/8) discordance with histopathology alone. Ig light chain was detected in 8 (80%) DLBCL cases and 40% (4/10) DLBCL cases showed Bcl-2 expression. Diagnosis of DLBCL by FCI on FNA of Lymph node can be of great help as in most cases of DLBCL biopsy of histopathology is not possible. Detection of Bcl-2 expression can help to assess the prognosis of the disease and resistance to chemotherapy.
Attenuation of Graft Arterial Disease by Manipulation of the LIGHT Pathway

Objective— The tumor necrosis factor (TNF) superfamily member LIGHT, which binds herpes virus entry mediator (HVEM) and lymphotoxin β receptor (LTβR), plays important roles in regulating the immune response. To clarify the mechanism underlying graft arterial disease (GAD), we investigated the role of the LIGHT pathway in the progression of GAD. Methods and Results— Hearts from Bm12 mice were transplanted into C57BL/6 (B/6) mice (class II mismatch). Recipients were injected intraperitoneally with HVEMIg (100 μg per treatment) every 7 days for 8 weeks. Treatment with HVEMIg significantly attenuated GAD (luminal occlusion=16.5±7.7% versus control allograft=62.6±12.1%, P<0.05), and significantly decreased intragraft IL-4, IL-6, and interferon-γ (IFN-γ) mRNA expression compared with controls. LTβR was expressed in smooth muscle cells (SMCs) with or without cytokine stimulation, whereas HVEM was detected in SMCs stimulated by IFN-γ. Coculture of SMCs with T cells after transplantation induced SMC proliferation,.
MODIFIKASI PRINSIP PEMERIKSAAN β-D-GLUCAN UNTUK MENDETEKSI CANDIDA ALBICANS DALAM SERUM

Abdominal obesity is closely linked to the occurrence of metabolic syndrome. In pathomechanism of metabolic syndrome, adipocity plays an important role as an active metabolic endocrine organ. This is accomplished the secretion of various hormones, enzymes, cytokines, and components that play a role in the rennin angiotensin system (RAS). One of the mechanisms linking the occurrence of hypertension in obesity is through the increased activity of RAS. Angiotensin II is the major effector of hypertension. The effect of high glucose exposure in adipocytes culture to angiotensin II secretion up to now is yet unknown. Adipociyties culture from rat visceral adipose tissue were exposed to 5 mM glucose concentration (as a physiological condition), 11 mM and 25 mM glucose concentration as the high glucose condition. Measurement of the angiotensin II level which is secreted in the culture medium was done by ELISA method. The mean (SD) levels of angiotensin II were 56.4 (4.28), 66.05 (2.24), and 69.22 (3:49) ng/mL respectively, for adipocities cultures exposed to 5 mM, 11 mM and 25 mM glucose concentration. High glucose exposure could increase the secretion of angiotensin II significantly in adipocytes culture. This suggests that the condition of hyperglycemia affects adipocytes dysfunction that play a role in the metabolic syndrome pathomechanism.
PCOI: Packet Classification‐Based Optical Interconnect for Data Centre Networks

To support cloud services, Data Centre Networks (DCNs) are constructed to have many servers and network devices, thus increasing the routing complexity and energy consumption of the DCN. The introduction of optical technology in DCNs gives several benefits related to routing control and energy efficiency. This paper presents a novel Packet Classification based Optical interconnect (PCOI) architecture for DCN which simplifies the routing process by classifying the packet at the sender rack and reduces energy consumption by utilizing the passive optical components. This architecture brings some key benefits to optical interconnects in DCNs which include (i) routing simplicity, (ii) reduced energy consumption, (iii) scalability to large port count, (iv) packet loss avoidance, and (v) all-to-one communication support. The packets are classified based on destination rack and are arranged in the input queues. This paper presents the input and output queuing analysis of the PCOI architecture in terms of mathematical analysis, the TCP simulation in NS2, and the physical layer analysis by conducting simulation in OptiSystem. The packet loss in the PCOI has been avoided by adopting the input and output queuing model. The output queue of PCOI architecture represents an M/D/32 queue. The simulation results show that PCOI achieved a significant improvement in terms of throughput and low end-to-end delay. The eye-diagram results show that a good quality optical signal is received at the output, showing a very low Bit Error Rate (BER).
Histone Methyltransferase SET1 Mediates Angiotensin II–Induced Endothelin-1 Transcription and Cardiac Hypertrophy in Mice

Objective—Endothelin-1 is a potent vasoconstrictor derived from vascular endothelium. Elevated endothelin-1 levels are observed in a host of cardiovascular pathologies including cardiomyopathy. The epigenetic mechanism responsible for endothelin-1 induction in these pathological processes remains elusive. Approach and Results—We report here that induction of endothelin-1 expression in endothelial cells by angiotensin II (Ang II) was accompanied by the accumulation of histone H3K4 trimethylation, a preeminent histone modification for transcriptional activation, on the endothelin-1 promoter. In the meantime, Ang II stimulated the expression and the occupancy of Suv, Ez, and Trithorax domain 1 (SET1), a mammalian histone H3K4 trimethyltransferase, on the endothelin-1 promoter, both in vitro and in vivo. SET1 was recruited to the endothelin-1 promoter by activating protein 1 (c-Jun/c-Fos) and synergized with activating protein 1 to activate endothelin-1 transcription in response to Ang II treatment. Knockdown.
Job Satisfaction and Work-Life Balance: Differences between Homework and Work at the Workplace of the Company

Working remotely can complement and sometimes completely substitute conventional work at the workplace of the company. Until the COVID-19 crisis the share of remote workers was relatively low and empirical investigations show inconsistent results. The recent work has highlighted a dramatic shift toward working from home The objective of this contribution is to empirically analyze the relationship between working remotely and job satisfaction on the one hand, as well as between working remotely and work-life balance on the other hand, based on three waves of the German Linked Personnel Panel. Our control variables are personality traits, skills, employment and job characteristics. We present average effects and demonstrate under which conditions remote work is advantageous for employees. Work-life imbalance may be induced by job-related causes. A private life can reduce work-life balance under specific conditions, namely, if remote work takes place outside of contracted working hours and during the first phase of remote work. On average, remote work has no significant impact on work-life balance, which is conditioned by private interests. However, the termination of remote work causes a clear imbalance. In contrast, the introduction of remote work increases job satisfaction, although only temporarily. When we compare employees working from home with those who want to work at home, we find that the former are happier. If we consider remote workers only, our results reveal that job satisfaction is higher, and work-life balance is not worse under a strict contractual agreement than under a nonbinding commitment.
How We Teach Vocabulary Matters: Extending Gesture'S Impact on Word Learning to Reading

The purpose of this study was to examine how learning words with matching and mismatching representational gesture affects subsequent comprehension of these newly-learned words within read sentential contexts. Results did not support our hypothesis that pseudowords learned via text and co-occurring semantically-congruent representational gestures would enhance subsequent identification and processing when reading them in sentential contexts. Implications are discussed.
Automatic migraine classification using artificial neural networks

Background: Previous studies of migraine classification have focused on the analysis of brain waves, leading to the development of complex tests that are not accessible to the majority of the population. In the early stages of this pathology, patients tend to go to the emergency services or outpatient department, where timely identification largely depends on the expertise of the physician and continuous monitoring of the patient. However, owing to the lack of time to make a proper diagnosis or the inexperience of the physician, migraines are often misdiagnosed either because they are wrongly classified or because the disease severity is underestimated or disparaged. Both cases can lead to inappropriate, unnecessary, or imprecise therapies, which can result in damage to patients’ health. Methods: This study focuses on designing and testing an early classification system capable of distinguishing between seven types of migraines based on the patient’s symptoms. The methodology proposed comprises four steps: data collection based on symptoms and diagnosis by the treating physician, selection of the most relevant variables, use of artificial neural network models for automatic classification, and selection of the best model based on the accuracy and precision of the diagnosis. Results: The artificial neural network models used provide an excellent classification performance, with accuracy and precision levels >97% and which exceed the classifications made using other model, such as logistic regression, support vector machines, nearest neighbor, and decision trees. Conclusions: The implementation of migraine classification through artificial neural networks is a powerful tool that reduces the time to obtain accurate, reliable, and timely clinical diagnoses.
Anatomia coronariana do gato-do-mato-pequeno (Leopardus guttulus)

Informações sobre a anatomia do sistema cardiovascular de carnívoros silvestres são escassas. O estudo da ramificação e dominância coronariana tem implicações filogenéticas e clínicas, embora em felídeos silvestres este conhecimento esteja restrito atualmente a poucas espécies. Objetivou-se descrever as artérias coronárias e seus principais ramos no gato-do-mato-pequeno (Leopardus guttulus) e comparar os achados com os de outras espécies da ordem Carnivora. Para tal, foram dissecados corações de dois espécimes, um macho e uma fêmea, recolhidos mortos em rodovias. A artéria coronária esquerda surgiu do seio esquerdo do bulbo aórtico e se bifurcou em um ramo interventricular paraconal e outro circunflexo, ambos originando ramos ventriculares. A artéria coronária direita surgiu do seio direito do bulbo aórtico e transitou pelo sulco interventricular como ramo interventricular subsinuoso, emitindo discretas ramificações ventriculares. Ambos os ramos interventriculares atingiram o ápice do coração sem anastomose entre si. Pode-se observar que a irrigação própria do coração do L. guttulus é do tipo equilibrada, sem dominância das artérias coronárias esquerda ou direita, o que difere das descrições para outras espécies da família Felidae.
The Perceptions of Primary School Teachers of Online Learning during the COVID-19 Pandemic Period: A Case Study in Indonesia

This study explores the perceptions of primary school teachers of online learning in a program developed in Indonesia called School from Home during the COVID-19 Pandemic. Data were collected through surveys and semi-structured interviews with 67 class teachers in primary schools. Data analysis used thematic analysis of qualitative data. The analysis results found four main themes, namely, instructional strategies, challenges, support, and motivation of teachers. This research contributes to the literature of online collaborative learning between teachers, parents, and schools that impact student success. Broadly, the success of online learning in Indonesia during the COVID-19 Pandemic was determined by the readiness of technology in line with the national humanist curriculum, support and collaboration from all stakeholders, including government, schools, teachers, parents and the community.
THEORY OF CHANGE AND LOGICAL FRAMEWORK: A COMPARATIVE MEASURE FOR MONITORING AND EVALUATION PRACTICES

Abstract. This article is a brief study of the advantages and disadvantages of Theory of Change (ToC) and the Logical Framework (Log Frame) based on literature; a comparative approach. The later has a long historical stand in the development practices while the former was formed as supposed to answer the shortcomings of the Log Frame. By comparing both strengths and weaknesses, the finding argues that ToC is strongly considered to have a more certain degree of advantages rather than the ME pendekatan komparatif. Yang kedua memiliki pendirian historis yang panjang dalam praktik pembangunan, sementara yang pertama dibentuk untuk menjawab kekurangan Log Frame. Dengan membandingkan kekuatan dan kelemahan, temuan ini berpendapat bahwa ToC secara kuat dianggap memiliki tingkat keuntungan yang lebih pasti daripada M&E dalam pandangan lembaga pembangunan terutama LSM dalam praktik pemantauan dan evaluasi praktik pembangunan. Monitoring & Evaluation secara khusus melahirkan ToC dalam rangka menjawab kekurangan Log Frame saat ini. ToC telah mendapatkan posisi positif di bidang pembangunan internasional dengan mengatasi setidaknya tiga fitur utama: partisipasi, fleksibilitas dan dinamika pembebasan dan akuntabilitas pembangunan.
A Novel Detection Method Based on Enhanced Diagonal Secant Updating Frequency Domain Fourth-Order Cumulant Scheme for DSSS Signals

In this paper, an asymptotic estimating technique to detect direct sequence spread spectrum (DSSS) signals using modified frequency domain fourth-order cumulant is proposed. Based on 2-D slices fourth-order cumulant, an efficient algorithm is proposed to solve the problem of complex computation and huge processing time. The method of fourth-order cumulant in frequency domain can achieve detection probability above 50% at SNR -25dB. In this paper, we strongly decrease processing time and complexity of computations that it makes this method appropriate for employing in the DSSS detection systems. This method only requires to store a row vector while ignoring all the low and diagonal elements and therefore the required memory is highly reduced. In addition, as it uses two-line search strategies (predictor and corrector) to obtain a new iterative point, the spectral properties of the diagonal updating scheme is improved, and rapid convergence property is gained. Also, this method estimates carrier frequency and symbol rate simultaneously in the online processing. Simulation results and computation analysis indicate that processing time of the proposed method is decreased about 30% with respect to the commonly used fourth-order cumulant methods.
Predictive factors for developing acute cholangitis and/or cholecystitis in patients undergoing delayed cholecystectomy: A retrospective study.

BACKGROUND /Objective: We evaluated the risk of acute cholangitis and/or cholecystitis while waiting for cholecystectomy for gallstones. METHODS We retrospectively enrolled 168 patients who underwent cholecystectomy for gallstones after conservative therapy. We compared clinical data of 20 patients who developed acute cholangitis and/or cholecystitis while waiting for cholecystectomy (group A) with 148 patients who did not develop (group B). We investigated surgical outcomes and risk factors for developing acute cholangitis and/or cholecystitis. RESULTS Preoperatively, significant numbers of patients with previous history of acute grade II or III cholecystitis (55.0% vs 10.8%; p < 0.001) and biliary drainage (20.0% vs 2.0%; p = 0.004) were observed between groups A and B. White blood cell counts (13500/μL vs 8155/μL; p < 0.001) and C-reactive protein levels (12.6 vs 5.1 mg/dL; p < 0.001) were significantly higher in group A than in group B; albumin levels (3.2 vs 4.0 g/dL; p < 0.001) were significantly lower in group A. Gallbladder wall thickening (≥5 mm) (45.0% vs 18.9%; p = 0.018), incarcerated gallbladder neck stones (55.0% vs 22.3%; p = 0.005), and peri-gallbladder abscess (20.0% vs 1.4%; p = 0.002) were significantly more frequent in group A than in group B. A higher conversion rate to open surgery (20.0% vs 2.0%; p = 0.004), longer operation time (137 vs 102 min; p < 0.001), and higher incidence of intraoperative complications (10.0% vs 0%; p = 0.014) were observed in group A, compared with group B. CONCLUSION A history of severe cholecystitis may be a risk factor for acute cholangitis and/or cholecystitis in patients waiting for surgery; it may also contribute to increased surgical difficulty.
Изменения в употреблении творительного инструментального в русском языке

The present study focused on the main changes in the use of the instrumental case that occurred in the Russian language in XI–XXI centuries and the factors that caused these changes. The study was conducted within the cognitivelinguistic framework or, more precisely, the theory of conceptual metaphor, according to which one conceptual domain is conceptualised and expressed in terms of another, more specific, domain. The data were collected from the Main Corpus of the National Russian Corpus, which contains sources of written language, mainly from original prose from XVIII century to the present-day Russian, as well as from the Old and Middle Russian sections of the Historical Subcorpora of the National Russian Corpus. The use of the instrumental case appeared to have been affected by the decreasing role of cases and the increasing role of prepositions in the Russian language, the transformation in the semantics of words used in constructions with the instrumental case, the impact of the syntactic patterns of synonyms and near-synonyms, and the influence of the syntactic patterns of borrowed words. The study also demonstrated the role of conceptual metaphors in the development of the instrumental meaning of the Russian instrumental case, as well as the development of the instrumental meaning of the synonymous syntactic structures. The instrumental meaning of the Russian instrumental case developed on the basis of the path metaphor. The instrument-as-a-path metaphor is very productive in the Russian language, which explains why other prepositional phrases, e.g. cherez+accusative or po+dative , developed instrumental meanings and took over some of the uses of the instrumental case.
ImmuneRegulation: a web-based tool for identifying human immune regulatory elements

Humans can vary considerably in their healthy immune phenotypes and in their immune responses to various stimuli. We have developed an interactive web-based tool, ImmuneRegulation, to enable discovery of human regulatory elements that drive some of the phenotypic differences observed in gene expression profiles. ImmuneRegulation currently provides the largest centrally integrated resource available in the literature on transcriptome regulation in whole blood and blood cell types, including genotype data from 23,040 individuals, with associated gene expression data from 30,562 experiments, that provide genetic variant-gene expression associations on ∼200 million eQTLs. In addition, it includes 14 million transcription factor (TF) binding region hits extracted from 1945 TF ChIP-seq peaks and the latest GWAS catalog of 67,230 published SNP-trait associations. Users can interactively explore ImmuneRegulation to visualize and discover associations between their gene(s) of interest and their regulators (genetic variants or transcription factors) across multiple cohorts and studies. These regulators can explain cohort or cell type dependent gene expression variations and may be critical in selecting the ideal cohorts or cell types for follow-up studies. Overall, ImmuneRegulation aims to contribute to our understanding of the effects of eQTLs and TFs on heterogeneous transcriptional responses reported in studies on the blood; in the development of molecular signatures of immune response; and facilitate their future application in patient management. ImmuneRegulation is freely available at http://icahn.mssm. edu/immuneregulation.
Effect of physically effective neutral detergent fibre and moisture content in fermented total mixed ration on lactating cow performance

This experiment aimed to determine the effectiveness of physically effective neutral detergent fibre as peNDF1.18 mm and the moisture levels of fermented total mixed rations (FTMR) in lactating cow performance. Eight crossbred Holstein cows with 430±17.1 kg BW in mid-lactation were used in a 4 × 4 replicated Latin square design (LSD). The treatments consisted of 20 and 25% physically effective neutral detergent fibre (peNDF) and 30 and 60% moisture contents. The results showed that DMI, and DMI of %BW0.75 were increased in 20 and 25% of peNDF with 30% moisture content. CP, EE, NDF, ADF and NFC digestibility was increased on 25% peNDF with 60% moisture content in FTMR. Acetate was increased in 25% peNDF (P less than 0.05), but propionate was higher in 20% peNDF compared to 25% peNDF. Milk fat percentage was increased in 25% peNDF. No differences were observed in blood glucose and BUN. Consequently, 20 and 25% peNDF1.18 mm with 60% moisture content in FTMR were the optimum levels for lactating cows.