Miracle-dz/UniFAR-FTUs huggingface dataset:
A Nanoparticle Platform for Accelerated In Vivo Oral Delivery Screening of Nucleic Acids

Oral administration of nucleic acids, such as small interfering RNAs and antisense oligonucleotides, represents a potent modality for treating many gastrointestinal (GI) diseases. However, their use is limited due to a lack of carriers that can effectively mediate their delivery through GI barriers, including gastric enzymes and mucus membranes. Lipid nanoparticles (LNPs) are an emerging delivery system that can protect nucleic acids from degradation and mediate their intracellular delivery, but few studies have evaluated their ability to overcome oral delivery barriers. Here, a high‐throughput in vivo screening platform is developed to accelerate the design of LNPs for oral delivery of nucleic acids. A library of LNPs that encapsulate DNA barcodes (b‐DNAs) was pooled and orally administered to mice, and their delivery was quantified by deep sequencing. Sequencing results indicated that increases in the molar ratio of cholesterol to lipid‐anchored polyethylene glycol conjugate in LNPs enhanced their GI content retention and that the presence of an LNP carrier improved b‐DNA delivery to GI tissues, relative to the delivery of naked b‐DNA. Collectively, these results suggest that high‐throughput in vivo screening can accelerate the discovery of LNPs for GI tract nucleic acid delivery upon oral administration.
Machine Learning Thermo‐Barometry: Application to Clinopyroxene‐Bearing Magmas

We introduce a new approach, based on machine learning, to estimate pre‐eruptive temperatures and storage depths using clinopyroxene‐melt pairs and clinopyroxene‐only chemistry. The model is calibrated for magmas of a wide compositional range, it complements existing models, and it can be applied independently of tectonic setting. Additionally, it allows the identification of the main chemical exchange mechanisms occurring in response to pressure and temperature variations on the base of experimental data without a priori assumptions. After the validation process, performances are assessed with test data never used during the training phase. We estimate the uncertainty using the root‐mean‐square error (RMSE) and the coefficient of determination (R2). The application of the best performing algorithm (trained in the range 0–40 kbar and 952–1882 K) to clinopyroxene‐melt pairs from primitive to extremely differentiated magmas of both subalkaline and alkaline systems returns a RMSE on the order of 2.6 kbar and 40 K for pressure and temperature, respectively. We additionally present a melt‐ and temperature‐independent clinopyroxene barometer in the range 0–40 kbar, characterized by a RMSE of the order of 3 kbar. Tested for tholeiitic compositions in the range 0–10 kbar, the melt‐ and temperature‐independent clinopyroxene barometer has a RMSE of 1.7 kbar. We finally apply the proposed approach to clinopyroxenes from Iceland, providing new, independent, insights about pre‐eruptive storage depths of Icelandic volcanoes. The general applicability of this model will promote the comparison between the architecture of plumbing systems across tectonic settings and facilitate the comparison between petrologic and geophysical studies.
Implementation of a Microcontroller-Based Chaotic Circuit of Lorenz Equations

Lorenz equations are commonly used in chaos education and studies. Simulation programs can be used to produce solutions of Lorenz equations and to examine its chaotic waveforms. However, sometimes a chaotic signal source can be needed. Such a circuit can be made using either analog or digital circuit components. Recently, a microcontroller-based circuit is suggested to obtain chaotic waveforms of Lorenz equations however only simulations are used to show proof of concept. Such circuit needs experimental verification. In this paper, implementation and experimental verification of the microcontroller-based circuit which solves Lorenz equations in real time and produces its chaotic waveforms are presented. Runge-Kutta method is used to solve the equation system. By using Proteus, microcontroller-based chaotic circuit is simulated and designed. Presented design has been implemented using an Arduino Mega 2560 R3 microcontroller. The microcontroller sends the chaotic signals to the outputs of the circuit using digital-to-analog converters. The waveforms acquired experimentally from the implemented circuit matches well with those obtained from Proteus simulations.
Structural and functional aspects of ¢lamins

Filamins are a family of high molecular mass cytoskeletal proteins that organize filamentous actin in networks and stress fibers. Over the past few years it has become clear that filamins anchor various transmembrane proteins to the actin cytoskeleton and provide a scaffold for a wide range of cytoplasmic signaling proteins. The recent cloning of three human filamins and studies on filamin orthologues from chicken and Drosophila revealed unexpected complexity of the filamin family, the biological implications of which have just started to be addressed. Expression of dysfunctional filamin-A leads to the genetic disorder of ventricular heterotopia and gives reason to expect that abnormalities in the other isogenes may also be connected with human disease. In this review aspects of filamin structure, its splice variants, binding partners and biological function will be discussed. fl 2001 Elsevier Science B.V. All rights reserved.
Phenacoccus solenopsis Tinsley (Sternorrhyncha: Coccoidea: Pseudococcidae), an invasive mealybug damaging cotton in Pakistan and India, with a discussion on seasonal morphological variation

Since at least 2005, a possibly introduced mealybug of the genus Phenacoccus has been causing serious damage to cotton (Gossypium hirsutum) over much of the Sindh and Punjab districts of Pakistan and in north-western India. Some short papers have been published locally giving details on the structure and biology of this species and suggesting the name Phenacoccus gossypiphilous Abbas, Arif & Saeed (2005) but without designating type specimens or depositories. This name is here considered a nomen nudum. A detailed morphological study has been unable to separate this species from many specimens of Phenacoccus from the Neotropics that are believed to be Phenacoccus solenopsis Tinsley. The material from the Indian subcontinent shows considerable morphological variation in the frequency of multilocular disc pores and oral collar tubular ducts on the ventral submargin of the abdomen; this appeared to be related to conditions under which this species was reared, with those cultured in a screen-house during the non-cotton-growing season being indistinguishable from P. solenopsis from the Neotropics . This paper re-describes the adult female of P. solenopsis Tinsley based on the type specimens from New Mexico, and designates a lectotype. The type material was compared with specimens considered to be P. solenopsis from elsewhere in the New World, and from West Africa and several areas in Asia, particularly Pakistan and India. Based of our present understanding of the morphology of adult female P. solenopsis, it is concluded that the species is rather variable, that this variability may be environmentally induced, and that the species currently causing widespread damage to cotton on the Indian subcontinent is referable to P. solenopsis. Adult male P. solenopsis from North America, Pakistan and India were also studied and again no significant differences were found. Descriptions are provided of the adult male and all the immature stages of P. solenopsis, based on material from India and Pakistan. Keys are provided to (a) separate P. solenopsis from similar species of Phenacoccus currently known from Asia and (b) to identify all instars. The morphological differences between P. solenopsis, P. solani Ferris and P. defectus Ferris are reviewed and, based on the morphological variation found in the Asian material, it is considered that there is some support for the suggestion that these three species might be environmentally induced variants of a single species. A few details are given of the biology of P. solenopsis on cotton in Pakistan.
A Web-Based Software Developed for Bayesian Tests and an Application in Medicine

Objective: In this study, it is aimed to develop a new user-friendly web-based software to easily carry out Bayesian tests, which are becoming more and more common, instead of using the classical approach, which is generally preferred in analysis from statistical modeling. Method: Shiny, an open-source R package, is used to develop the recommended web software. In the developed software, by selecting “the Specify Sample Number” tab, the number of samples presented as “Single”, “Two” options is selected, and analyzes are made by selecting the appropriate data set from the file upload menu. Results: The data set “ulcer recurrence” was used to examine the way the developed web-based software works and to evaluate its output. To test whether there is a difference in age variable in terms of result variable, “Two Independent Sample Bayes Tests” were selected and analyzes were performed. According to the results obtained, statistically “little evidence for Ho” was found for the age variable in terms of the result variable. With the evidence obtained, it is said that no statistically significant difference was obtained for the dependent variable according to the independent variable. Conclusion: The developed software is a new user-friendly web-based software that can be used to easily use Bayesian tests used as an alternative to the classical approach.
Trait-Based Root Phenotyping as a Necessary Tool for Crop Selection and Improvement

Most of the effort of crop breeding has focused on the expression of aboveground traits with the goals of increasing yield and disease resistance, decreasing height in grains, and improvement of nutritional qualities. The role of roots in supporting these goals has been largely ignored. With the increasing need to produce more food, feed, fiber, and fuel on less land and with fewer inputs, the next advance in plant breeding must include greater consideration of roots. Root traits are an untapped source of phenotypic variation that will prove essential for breeders working to increase yields and the provisioning of ecosystem services. Roots are dynamic, and their structure and the composition of metabolites introduced to the rhizosphere change as the plant develops and in response to environmental, biotic, and edaphic factors. The assessment of physical qualities of root system architecture will allow breeding for desired root placement in the soil profile, such as deeper roots in no-till production systems plagued with drought or shallow roots systems for accessing nutrients. Combining the assessment of physical characteristics with chemical traits, including enzymes and organic acid production, will provide a better understanding of biogeochemical mechanisms by which roots acquire resources. Lastly, information on the structural and elemental composition of the roots will help better predict root decomposition, their contribution to soil organic carbon pools, and the subsequent benefits provided to the following crop. Breeding can no longer continue with a narrow focus on aboveground traits, and breeding for belowground traits cannot only focus on root system architecture. Incorporation of root biogeochemical traits into breeding will permit the creation of germplasm with the required traits to meet production needs in a variety of soil types and projected climate scenarios.
Evaluation of chemicals and antibiotics against green mold and bacterial rot of Pleurotus spp

The genus Pleurotus is most exploitable xylotrophic fungi, with valuable biotechnological, medical, and nutritional properties. The relevant features of the representatives of this genus to provide attractive lowcost industrial tools have been reported in numerous studies to resolve the pressure of ecological issues. Additionally, a number of Pleurotus species are highly adaptive, do not require any special conditions for growth, and possess specific resistance to contaminating diseases and pests.Even then species of this edible mushroom can often be affected by some bacterial, mold and virus diseases that rather frequently cause dramatic production loss.In the present experiment we evaluated the efficacy of different chemicals and antibiotics against green mold and bacterial rot of Pleurotus florida and Pleurotus djamor. From this experiment it was concluded that the maximum number of fruiting body in both Pleurotus spp. are taking in the spray of Streptomycin 400 ppm + Carbandazim 0.0025% (21.67 and 22.33), respectively.
ANALYSIS ON CONSTRAINTS OF LOCAL AND NATIONAL LANGUAGE USING IN LATE ADOLESCENCE IN NORTH SUMATRA

Indonesia is famous for its rich and varied language culture which is influenced by the cultural backgrounds, ethnicities, and customs of each region. From 718 local languages in Indonesia, there are 5 of them in North Sumatra, namely: Batak, Nias, Minang, Java, and Malay language. Therefore, national language is needed to unite the various tribes, especially for late adolescence in their daily activities both those from city to village or from village to city. This difficulty was the basis of our research to analyze the constraints of using two languages (local and national) in late adolescence in North Sumatra, Indonesia. Sumatra, such as basic sentence patterns, language intonation, dialect, cultural background, and habits.
Degradation State Recognition of Piston Pump Based on ICEEMDAN and XGBoost

Rui Guo 1,2,* , Zhiqian Zhao 1,3, Tao Wang 1,4, Guangheng Liu 1,4, Jingyi Zhao 2,4 and Dianrong Gao 3 1 Hebei Provincial Key Laboratory of Heavy Machinery Fluid Power Transmission and Control, Yanshan University, Qinhuangdao 066004, China; zzq623310996@163.com (Z.Z.); m13237195956@163.com (T.W.); lgh18733407119@163.com (G.L.) 2 The State Key Laboratory of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou 310027, China; zjy@ysu.edu. cn 3 Key Laboratory of Advanced Forging and Stamping Technology and Science, Yanshan University, Qinhuangdao 066004, China; gaodr@ysu.edu. cn 4 Hebei Key Laboratory of Special Delivery Equipment Yanshan University, Qinhuangdao 066004, China * Correspondence: guorui@ysu.edu. cn
In Situ estimate of ultimate tensile strength for part optimization in extrusion-based additive manufacturing

Uncertainty in the final mechanical characteristics of an extrusion-based additive manufacturing process is a major challenge for the field. Estimating these mechanical characteristics of the specimen during printing can save cost and resources, allowing for the application of optimization methods to correct for natural error. We propose a deep learning based computer vision approach to continuously estimate the tensile strength using recent images of each printed layer, and acting to critique the efficacy of the extrusion process. This predictive model is useful for in situ part optimization, maximizing the output prediction.
Research on the properties of modified water treatment agent for white pulp of pomelo peel

a water treatment agent for white pulp of pomelo peel was developed using commercial Pomelo as raw material, which laid a foundation for the further development and utilization of Pomelo Peel. Indeed adsorption Cu2+, Fe3+ as an index, the effect of modification on the basis of single factor experiment research on the use of sodium hydroxide concentration, pH, adsorbent, adsorption process the time required to the influence of factors such as the adsorption effect, orthogonal experiment as the main experimental method to determine the modification of pomelo peel white pulp desorption process using the adsorption effect of again. The results showed that the modified water treatment agent had higher adsorption efficiency for Cu2+ and Fe3+ in water, and had favourable reabsorption ability, it can be reused and has higher adsorption efficiency. The developed water treatment agent for white pulp of Pomelo Peel has laid a foundation for the adsorption of Cu2+ and Fe3+ in water, the efficient utilization of white pulp of pomelo peel and the invention of production equipment.
Analysis of lung cancer morbidity and mortality based on particle swarm optimization

Based on the particle swarm algorithm to establish a mathematical model, this article counts the air quality index, tobacco sales, the number of cancer hospitals and the number of health personnel from 2008 to 2019, and quickly calculates the morbidity and mortality of lung cancer over the years, and uses statistics The relative error between the value and the calculated value is the evaluation standard. The result proves that the relative error between the calculated value and the statistical value of the morbidity and mortality of lung cancer is less than 10%, indicating that the calculation result of the particle swarm algorithm has good confidence.
Ostreopsis cf. ovata and Ostreopsis lenticularis (Dinophyceae: Gonyaulacales) in the Galapagos Marine Reserve

The genus of benthic dinoflagellates Ostreopsis is of particular interest because some species negatively impact human health and coastal marine ecosystems. Ostreopsis populations from a remote area, such as the Galapagos Marine Reserve with its unique biodiversity, can provide significant data. Samples of epibionthic dinoflagellates were collected from two islands (Santa Cruz and Santa Fe) in 2017. Species of the genera Gambierdiscus, Amphidinium, Coolia and Ostreopsis were found. Ostreopsis strains were isolated to characterize their morphology, molecular biology and toxicity. Three dif­ferent morphotypes of Ostreopsis based on dorsoventral and width diameters (n=369) were distinguished. The small cell morphotype was dominant in ten samples, with abundances of up to 33405 cells g-1 fresh weight of macroalgae. A total of 16 strains were isolated from field samples with subsequent polymerase chain reaction amplifications of rDNA, 5.8S rDNA and internal transcribed space regions; 13 strains (small cell morphotype) clustered in the O. cf. ovata Atlantic/Indian/Pacific clade; and 3 strains (large cell morphotype) clustered in the Ostreopsis lenticularis genotype from the type locality. The strains proved to be non-toxic. The presence of these genera/species represents a potential threat to marine ecosystems, and it is thus important to consider benthic species in the surveillance of harmful algae blooms in the reserve.
Analyzing spatial analytics systems based on Hadoop and Spark: A user perspective

Spatial analytics systems (SASs) represent a technology capable of managing huge volumes of spatial data using frameworks such as Apache Hadoop and Apache Spark. An increasing number of SASs have been proposed, requiring a comparison among them. However, existing comparisons in the literature provide a system‐centric view based on performance evaluations. Thus, there is a lack of comparisons based on the user‐centric view, that is, comparisons that help users to understand how the characteristics of SASs are useful to meet the specific requirements of their spatial applications. In this article, we provide a user‐centric comparison of the following SASs based on Hadoop and Spark: Hadoop‐GIS, SpatialHadoop, SpatialSpark, GeoSpark, GeoMesa Spark, SIMBA, LocationSpark, STARK, Magellan, SparkGIS, and Elcano. This comparison employs an extensive set of criteria related to the general characteristics of these systems, to the aspects of spatial data handling, and to the aspects inherent to distributed systems. Based on this comparison, we introduce guidelines to help users to choose an appropriate SAS. We also describe two case studies based on real‐world applications to illustrate the use of these guidelines. Finally, we discuss chronological tendencies related to SASs and identify limitations that SASs should address to improve user experience.
Characterisation of Campylobacter jejuni Lipooligosaccharides

Campylobacter jejuni is a major bacterial cause of food-borne enteritis, and its lipooligosaccharide (LOS) plays an initiating role in the development of the autoimmune neuropathy, Guillain-Barre syndrome, by induction of anti-neural cross-reactive antibodies through ganglioside molecular mimicry. Herein we describe the existence and heterogeneity of multiple LOS forms in C. jejuni strains of human and chicken origin grown at 37°C and 42°C. The C. jejuni NCTC 11168 original isolate (11168-O) was compared to the genome-sequenced variant (11168-GS), and both were found to have a lower-Mr LOS form, which was different in size and structure to the previously characterized higher-Mr form bearing GM1 mimicry. The lower-Mr form production was found to be dependent on the growth temperature as the production of this form increased from ~5 %, observed at 37°C to ~35 % at 42°C. The structure of the lower-Mr form contained a Galβ1,3GalNAc disaccharide moiety which is consistent with the termini of the GM1, asialo-GM1, GD1, GT1 and GQ1 gangliosides, however, it did not display GM1 mimicry as assessed in blotting studies but was shown by NMR analysis to resemble asialo-GM1. The production of multiple LOS forms and lack of GM1 mimicry was not a result of phase variation in the genes tested for NCTC 11168 and was also observed in most of the human and chicken isolates of C. jejuni tested.
Antiproliferative and apoptotic activity of Crude Skin Secretion from Malaysian Toad (Bufo asper) on in vitro colorectal cancer cells.

Earlier, a protein profile of Bufo asper skin secretion had been identified. The crud skin extract possessed antimicrobial activity against wide range of pathogenic bacteria. In the present communication, the anti-proliferative and apoptogenic properties of toads skin secretion (TSS) have been evaluated in colorectal cancer cell line. The anti-proliferative activity of TSS was tested over HCT 116 and CCD-18Co cells by MTT assay. Hoechst and rhodamine staining and caspases 3 and 7 were evaluated in order to verify cell death and apoptosis. TSS exhibited potent dose-dependent cytotoxic effect against human colorectal cancer cell line HCT 116 cells with an IC50 (15± 1.19 μg/mL). Conversely, the viability of normal colonic fibroblasts cell line CCD-18Co was much less affected by similar doses of TSS. Morphological studies by microscope on HCT 116 cells indicated certain events of apoptosis such as nuclear shrinkage, membrane blebbing and vacuolization of cells. This was further supported by activation of caspases 3 and 7. Considering the growing interest of developing naturally occurring host-defense peptides for new anticancer agents, data presented in this work strongly reinforce the validity of Bufo asper skin secretion as a rich source of new anticancer compounds.
Study on distribution characteristics of wireless charging system coupling mechanism

Coupling mechanism is one of the key issues in the research of electric vehicle wireless charging system. Single D and DD are two kinds of coupling mechanism commonly used in wireless charging system, and spatial magnetic field distribution are different depending on the coupling mechanism. In this paper, the expressions of magnetic flux density at any point in a single D and DD were derived. A 3D magnetic field measurement platform was built and the magnetic field distribution was drawn. The experimental results show that in the primary test, single D type magnetic induction intensity is 1.18 times of DD type, with the increase of the transmission distance, DD type coupling mechanism of magnetic induction intensity attenuation is slow, and on the secondary side of DD type magnetic induction intensity is 1.12 times of D type.