Miracle-dz/UniFAR-FTUs huggingface dataset:
Plant Growth Regulators Reduce Postproduction Leaf Yellowing of Potted Asiflorum Lilies

The postharvest quality of potted Asiflorum lily 'Donau' (Lilium hybrid) was evaluated after plants were sprayed with 0, 50, 250, or 500 mg.L -1 (BA equivalent) of Promalin (GA 4+7 to BA ratio was 1:1) or Accel (GA 4+7 to BA ratio 1:10) and stored at 2 to 3 °C for 0, 10, or 20 days. As storage was prolonged, more leaves senesced once plants were removed for evaluation. Leaf senescence declined with increasing concentrations of either Promalin or Accel, but Promalin was more effective. Application of 250 mg.L -1 Promalin completely eliminated leaf senescence over the 20-day shelf-life evaluation period, irrespective of duration of cold storage. The treatments did not affect flower bud opening or plant height. Chemical names used: gibberellin (GA 4+7 ); benzyladenine (BA). ).
Study of Apoptosis by Scirpi Tuber in Hela Cell and MCF-7 Cell

Objectives: This study was designed to investigate the analysis of apoptosis by Scirpi Tuber in Hela cell and MCF-7 cell. Methods: For cytotoxic effect of Scirpi Tuber extract, Scirpi Tuber extract were cultured on NIH3T3 cell in vitro. After treatment with various concentration of Scirpi Tuber, cell growth was evaluated in Hela cell and MCF-7 cell. Hoechst 33342 staining was performed to estimate DNA fragment effect of Scirpi Tuber on the apoptosis in Hela cell and MCF-7 cell. Annexin V/PI apoptosis assay was used to estimate the effects of Scirpi Tuber on the early apoptosis in Hela cell MCF-7 cell. All the stained cells were analyzed by a FACS. RT-PCR was used to estimate the apoptosis gene expression effect of Scirpi Tuber extract on Hela cell and MCF-7 cell. Results: Cytotoxic effect of Scirpi Tuber extract was not found on per NIH3T3 cell. The viability of Hela cell was significantly decreased Scirpi Tuber (500, ) in Hela cell 1day, 3day and 5days after treatment (p) in MCF-7 cell (p) in MCF-7 cell only 3days after treatment (p of ACR extract, BCL-2 were decreased and BAX, caspase-3 were increased both in Hela cell and MCF-7 cell. DNA fragmentation was observed the Scirpi Tuber on Hela cell and MCF-7 cell. As time goes on DNA fragmentation incresed. In Annexin V/PI apoptosis assay, after treatment of of Scirpi Tuber, the early apoptotic cell increased both in Hela cell and MCF-7 cell. As time goes on apoptotic cell increased. Conclusion: Scirpi Tuber appears to have considerable activity on the apoptosis in Hela cell and MCF-7 cell.
Transforming growth factor (TGF)-β levels and unprovoked recurrent venous thromboembolism Running title: TGF-β and recurrent venous thromboembolism

Prediction of recurrence in patients with unprovoked venous thromboembolism (VTE) remains a challenge. Studies of atherosclerosis suggest a protective role of transforming growth factor (TGF)-β. However, the role of TGF-β has not been studied in VTE. The aim of this study was to investigate TGF-β as a predictive marker of recurrent VTE in patients with a first episode of unprovoked VTE. Patients in the Malmö Thrombophilia Study (MATS) were followed after the discontinuation of anticoagulant treatment until the diagnosis of recurrent VTE or the end of the study in December 2008 (mean±SD 38.5 months ±27). Among patients with a first episode of unprovoked VTE, we identified 42 patients with recurrent VTE during the follow-up period. Two ageand sex-matched control subjects without recurrent VTE were selected for each patient (n=84). Plasma levels of the three isoforms of TGF-β (TGF-β1, TGFβ2 and TGF-β3) were quantified simultaneously by TGF-β 3-plex immunoassay. Compared to controls, plasma levels of TGF-β1 and TGF-β2 were significantly lower in patients with recurrent VTE (p<0.05), whereas no difference was found for TGF-β3. In a multivariate Cox regression analyses, adjusted for inherited thrombophilia, age, sex and BMI, low levels of TGF-β1 (hazard ratio [HR] =2.2, 95% confidence interval [CI]=1.1-4.3; p=0.02) and TGF-β2 (HR=2.4, 95% CI=1.2-4.7; p=0.01) were independently associated with a higher risk of recurrent VTE. We propose TGF-β1 and TGF-β2 as potential predictive markers for recurrence in patients with unprovoked VTE.
Identifying information from data using an organizational goals ontology: a case of the Australian economy / Tengku Adil Tengku Izhar and Torab Torabi.

Organizational data is important to assist domain experts and entrepreneurs for decision making process in relation to the organizational goals. The trustworthiness of organizational data in relation to achieving the organizational goals is often questioned because of the vast amount of organizational data. This paper proposes a methodology to evaluate organizational data that relates to the organizational goals. This refers to the importance of assisting the organization to utilize relevance of organizational data from the vast amount of datasets for decision-making in relation to the organizational goals. The aim of this paper is to evaluate the level of the organizational goals achievement. In order to achieve this aim, we identify the dependency relationship between organizational data and organizational goals. Based on this dependency relationship, we define a metrics to analyse organizational data to be considered relevant to the organizational goals achievement. The case study is present to test the applicability of the methodology to measure the level of the Australian economy. The results show the dependency relationship between the case study goal and its variables. The contribution of this paper will serve as a new approach in evaluating the level of the organizational goals achievement.
The impact of wood ants (Hymenoptera: Formicidae) on the structure of invertebrate community on mountain birch (Betula pubescens ssp. czerepanovii)

We studied the effect of wood ants on the mountain birch canopy-invertebrate community by sampling a total of 840 trees at different altitudes on the mountain slopes in northeastern Lapland, Finland. We also estimated thedegree of herbivory on birch leaves, and monitored the prey of the wood ants. We addressed three questions: (I) How invertebrates are distributed along the altitudinal gradient and on a larger regional scale, (2) how wood ants (Formica aquilonia) affect the distribution of herbivores and predators in the mountain birch canopy, and (3) how the potential prey of wood ants vary in relation to altitude, season and year. Our results demonstrate that the number of sawfly larvae and the degree of herbivory on birch leaves increases with elevation, with the largest impact on mid elevation areas previously damaged during autumnal-moth (Epirrita autumnata) outbreaks. Wood ants and ant-tended aphids (Symydobius oblongus) were most abundant at lower elevations. Wood ants affected most invertebrate groups negatively at all elevations where the wood ants existed, and the effect was quite stable in time. Further, herbivores were found to comprise a large proportion of prey of wood ants. Because birches are often killed during the outbreak years of moths, trees gain long-term protection by wood ants at reasonably low yearly costs - growth reduction owing to sap sucking by ant-tended aphids. Through this protection, wood ants may prevent the recession of the timberline during outbreak years. Further, defoliated forests may recover substantially faster when birch propagules spread from the green islands of birches surviving around the ant mounds.
Suspended silt and salinity tolerances of the first zoeal stage of the fiddler crab Uca annulipes (Decapoda: Brachyura) and why marine connectivity is essential to the survival of the species

Fiddler crabs (Uca spp.) undergo the zoeal stage of development in open-ocean waters, where they experience stable salinity levels, low turbidity and reduced predation. The St Lucia estuarine system has undergone many geomorphological changes, both natural and anthropogenic, and the estuary mouth has been closed since the early 2000s. Despite recent attempts to improve marine connectivity, it remains limited, occurring primarily on the flood tide through channels connected to the adjacent Mfolozi River. Larval export from the St Lucia Estuary is therefore almost non-existent. A laboratory study was undertaken to examine the silt and salinity tolerance of Uca annulipes first stage zoeae, to investigate whether survival in the closed-estuary conditions would be possible. Salinity tolerance was narrow, with zoeae displaying 100% mortality at salinities <20 and >35 after five days. Zoeae were widely tolerant to silt loading and did not display a significant decrease in survival over a range of 0–1 000 NTU. A limited salinity tolerance is in accordance with the life-history strategy of fiddler crabs, and a high tolerance to turbid waters can be advantageous to small-bodied merozooplankton. Given the stenohaline nature of the zoeae, marine connectivity is therefore essential for the persistence of U. annulipes in this estuarine habitat.
Complexity Measures in Software Engineering: A Systematic Comparison and Evaluation on Software-Component-Level

There are many measures on classand statement-level which assess different characteristics of software systems. However, the selection and diversity of package-level measures, especially of those focusing on the dependencies of a software system, is very limited. This is problematic because the complexity of a large software system emerges from the dependencies between the components of the system, i.e., we need to be able to measure these dependencies with regard to different characteristics of the dependency graph of a system. Therefore, we propose and evaluate several package-level measures that cover several of these characteristics. For this purpose we use Weyuker’s Properties to conduct a formal analysis of the measures and implement the prototypical measurement tool Jade to evaluate the applicability of the measures on the example of CPAchecker. Based on the analysis we show that three of the proposed measures DCMCC , DLM and P-DepDegree are applicable and useful for measuring the complexity of a system based on its dependencies.
Distribution of glycosaminoglycans in the developing facial region of mouse embryos.

: Synthesis and distribution of glycosaminoglycans in the developing facial region of mouse embryos were investigated during stages of development when the embryonic facial processes form and expand. Facial regions of 11th and 12th day embryos were either 1) labeled with 3H-glucosamine and newly synthesized glycosaminoglycans determined on the basis of specific enzyme sensitivity, or 2) sectioned and stained with Alcian Blue coupled with enzyme digestion in order to localize glycosaminoglycans. On the basis of enzyme sensitivity over half of the newly synthesized glycosaminoglycans during this time period were identified as hyaluronate, with only 12-15% representing chondroitin sulfate. Hyaluronate was found to be the primary component of the extracellular matrix. Basal laminae exhibited prominent chondroitin sulfate staining and also appeared to contain hyaluronate and heparan sulfate. It is suggested that hyaluronate may play a structural role in maintaining the shape and orientation of the facial processes.
Peptides derived from a sequence within β3 integrin bind to platelet αIIbβ3 (GPIIb-IIIa) and inhibit ligand binding

Peptides derived from a sequence within the loop structure of human platelet glycoprotein (GP) IIIa (integrin β 3 ) were previously shown to inhibit fibrinogen binding to purified GPIIb-IIIa. In this study a series of peptides based on the GPIIIa sequence 21-221 (SVSRNRDAPEG) was synthesized. The most active peptide was determined to be RNRDA, and its inhibitory potency was 4-fold greater (IC 50 =4.8 μM) than that of SVSRNRDAPEG. These GPIIIa peptides also inhibited the binding of two monoclonal antibodies, pl-55 and PAC-1, which are directed against the activated conformer of GPIIb-IIIa
Identification Of Compounds Of Allelopathic Extracts From Two Species Of Metapeyssonnelia (Rhodophyta) Growing On The Hydrocoral, Millepora Complanata, In Puerto Rico

Two Puerto Rican species of the encrusting red alga Metapeyssonnelia are known to overgrow and kill the hydrocoral Millepora complanata. The overgrowth is accompanied by bleaching or tissue lightening regions surrounding the growing margin of the algae on the coral tissue. Lipophilic and hydrophilic extracts from Metapeyssonnelia corallepida and M. milleporoides were obtained and analyzed by GC—MS. The following compounds were identified from M. corallepida: squalene and 5—(hydroxymethyl)—2—(dimethoxymethyl)—furan3— methoxycarbonyl—1,1—diethyl—2—buten—4—olide and from M. milleporoides: 2,4—dit—butyl phenol, 2(3H)—furanone and dihydro—4,4—dimethyl— (CAS), terpenes, furanones and phenol. Aliquots of crude extracts of both Metapeyssonnelia species applied to coral fragments resulted in visible changes to the coral tissue as well as unexpected change in numbers of zooxanthellae. The highest concentration of M. corallepida lipophilic extracts assayed generated bleaching halos, death of coral tissue and a reduction of the zooxanthellae number while the two lower concentrations of lipophilic extracts of M. milleporoides resulted in obvious tissue lightening, but without decline in zooxanthellae number.
Cloning of monoacylglycerol o-acyltransferase 2 cDNA from a silkworm, Bombyx mori

Abstract Monoacylglycerol O-acyltransferase 2 (MOGAT2) plays critical roles in lipid homeostasis. We reported a cDNA designed BmMOGAT2 encoding an MOGAT2 homologue cloned from the fat body of the silkworm Bombyx mori, by using conserved domain homology search method. The resultant BmMOGAT2 was translated to a protein encoding 352 amino acids with a theoretical isoelectric point of 9.04 and a molecular weight of 39,944.48 Da. Homology analysis revealed that BmMOGAT2 exhibits higher similarity on the amino acid level to those of other species already reported; 48% identity with Homo sapiens, 46% with Mus musculus, 50% with Danio rerio, and 42% with Drosophila melanogaster. The expression of BmMOGAT2 was detected in all tissues tested with 2-fold higher expression in the post-silk gland, as compared to others, and stronger expression of the larval fat body at 1st instar, as compared with other stages. The BmMOGAT2 is a predicted endoplasmic reticulum (ER) transmembrane protein with two ER transmembrane domains; BmMOGAT2 gene expression increases in response to ER stress-inducible drugs. To our knowledge, this is the first report of Bombyx mori MOGAT2 cDNA, BmMOGAT2, and its associated molecular characterization.
Review Article A Practical and Comprehensive Immunohistochemical Approach to the Diagnosis of Superficial Soft Tissue Tumors

Soft tissue tumors include neoplasms of specific and unknown lineages, and, therefore, lineage markers of smooth muscle, skeletal muscle, endothelial, epithelial and Schwann cells have proven useful in everyday practice. However, groups of tumors remain that are defined essentially on grounds of histology; others can be defined by molecular genetic studies. The complex distribution patterns of many antigens and loss of some differentiation antigens in malignant tumors often necessitate the use of panels of antibodies. Optimally such panels should address all significant differential diagnostic alternatives. There is little doubt that numerous new differentiation markers will appear in the future. The evaluation of tumor proliferation, apoptosis, and cell cycle control will give new information related to tumor biology and prognosis.
Is there a Specific Ecological Niche for Electroactive Microorganisms

The core of primary microbial electrochemical technologies (METs) is the ability of the electroactive microorganisms to interact with electrodes via extracellular electron transfer (EET), allowing wiring of current flow and microbial metabolism. Geobacter sulfurreducens and Shewanella oneidensis are the model organisms for understanding and engineering EET. Many other microorganisms are reported being electroactive but are often sparsely characterized. Based on a literature survey 94 species are ascribed as electroactive. Their apparent diversity raises questions on the natural importance and distribution of the EET capacity, that is, of the ecological niche of microbial electroactivity. To identify this potential niche the environmental preferences and natural habitat characteristics of all electroactive species were combined with their metabolic, growth and EET characteristics and an extensive meta-analysis performed. The results indicate that there is not a single ecological niche for electroactive microorganisms. Significantly more electroactive species presumably exist in nature as well as already existing strain collections but due to current cultivation techniques their EET potential is not leveraged. Thus, in the light of specific traits required for industrial application, microbial resource mining based on ecological knowledge bears a great potential for broadening the foundation of microbial electrochemistry as well as for future developments of primary METs.
Plant essential oils as green pesticides for pest and disease management

Certain plant essential oils show a broad spectrum of activity against pest insects and plant pathogenic fungi, and some oils have a long tradition of use in the protection of stored products. Recent investigations indicate that some chemical constituents of these oils interfere with the octopaminergic nervous system in insects. As this target site is not shared with mammals, most essential oil chemicals are relatively non-toxic to laboratory animals and fish in toxicological tests, and meet the criteria for "reduced risk" pesticides. Some of these oils and their constituent chemicals are widely used as flavoring agents in foods and beverages and are even exempt from pesticide registration in the United States. This special regulatory status combined with the wide availability of essential oils from the flavor and fragrance industries, has made it possible to fast-track commercialization of essential oil-based pesticides in the U.S.A. Though well received by consumers for use against home and garden pests, these "green pesticides" can also prove effective in agricultural situations, particularly for organic food production.
Limited potential for mosquito transmission of a live, attenuated chikungunya virus vaccine.

: Studies were conducted to determine the potential for transmission of a live, attenuated chikungunya (CHIK) virus vaccine by orally exposed or virus-inoculated mosquitoes. The vaccine (CHIK 181/clone 25) replicated in and was transmitted by female Aedes albopictus and Ae. aegypti after intrathoracic inoculation. Mosquitoes also became infected with the vaccine after ingesting virus from either a blood-soaked cotton pledget or a viremic monkey. However, because of the low viremias produced in inoculated humans, it is unlikely that mosquitoes would become infected by feeding on a person inoculated with the live, attenuated CHIK vaccine. Although the vaccine was transmitted by mosquitoes after intrathoracic inoculation, there was no evidence of reversion to a virulent phenotype.
Energy balance of chosen crops and their potential to saturate energy consumption in Slovakia

The aim of the present work was to assess and compare energy inputs and outputs of various crop managements in 2011–2012. Two main crops on arable land and three permanent grasslands were investigated. Silage maize (Zea mays L.) and winter wheat (Triticum aestivum L.) were grown on lowland, whilst two semi-natural grasslands and grassland infested by tufted hair-grass (Deschampsia caespitose (L.) P. Beauv) were located in mountainous regions of Slovakia. In these crops and grasslands the dry matter yield was measured and subsequently the supplementary energy, energy gain and unifying energy value – tonne of oil equivalent (TOE) – were calculated. Silage maize with 233.37 GJ*ha-1 has provided the highest energy gain. In the group of grasslands, grassland infested by tufted hair-grass has offered the highest energy gain (59.77 GJ*ha-1). And this grassland had the lowest requirement on the supplementary energy (3.66 GJ*ha-1), contrary to silage maize with highest one (12.37 GJ*ha-1). The total energy potential of the crop biomasses was confronted with energy consumption in Slovakia. Winter wheat has the biggest energy potential, but it could cover only 19.6% and 11.3% total consumption of electricity or natural gas, respectively. Large area of permanent grasslands and their spatial location make them an important energy reservoir for bioenergy production. But, it is not possible to replace all consumed fossil fuels by bioenergy from these tested renewable energy sources.
Modulation of Synaptic Vesicle Pools by Serotonin and the Spatial Organization of Vesicle Pools at the Crayfish Opener Neuromuscular Junction

Supervisory Committee Dr. Kerry R. Delaney (Department of Biology) CoSupervisor Dr. Patrick C. Nahirney (Division of Medical Sciences) Co-Supervisor Dr. Raad Nashmi (Department of Biology) Departmental Member The crayfish claw opener neuromuscular junction (NMJ) is a biological model for studying presynaptic neuromodulation by serotonin and synaptic vesicle recycling. Serotonin acts on crayfish axon terminals to increase the release of the neurotransmitter glutamate, but a complete understanding of its mechanisms of action are unknown. In order to sustain enhanced neurotransmission over long periods of time, it was hypothesized that serotonin recruits (activates) a population of previously non-recycling vesicles to become releasable and contribute to neurotransmission. To determine if serotonin activates a distinct population of synaptic vesicles, FM1-43 fluorescence unloading experiments were performed on crayfish excitatory opener axon terminals. These experiments could not resolve a serotonin-activated population of synaptic vesicles, but instead revealed that synaptic vesicles change behaviour in axon terminals independent of serotonin, with vesicles becoming less likely to exocytose and unload FM1-43 dye over time. The change in behaviour was hypothesized to be due to conversion of vesicles from a recycling (releasable) status to a reserve (reluctant to release) status. Synaptic vesicle pool localization was then tested using photoconversion of FM1-43 and transmission electron microscopy techniques. The spatial location of FM1-43-labeled vesicles fixed 2 minutes following 20 Hz stimulation did not reveal retention of vesicles specifically near release sites and the distribution of FM1-43-labeled vesicles was not significantly different between early (2 min) and late (180 min) time points. Terminals fixed 30 seconds following stimulation, however, contained numerous endosome-like structures the most frequently observed structures resembled large vesicles, which were the equivalent of 2-5 regular vesicle sizes. These results suggest that crayfish axon terminals recycle vast amounts of membrane in response to sustained 20-Hz stimulation and endocytosis appears to occur via multiple routes with the most common being through large vesicle intermediates.
[Impaired interferon status in children with acute respiratory infection and its correction with bifidumbacterin-forte].

: The state of interferon status was studied in 46 hospitalized children: 33 patients with complicated forms of acute respiratory virus infection (ARVI), such as pneumonia, bronchitis, etc., and 13 patients with vegetovascular dystonia (used as a comparison group). The study revealed that in patients with acute infections of the upper and lower respiratory tract considerable changes in their interferon system were registered. Children with ARVI were treated with Bifidumbacterin forte, a probiotic preparation, in large doses. Bifidumbacterin forte was found to produce a regulatory effect on the interferon system by enhancing the induction of alpha- and gamma-interferon and decreasing the production of serum interferon. The experience of using Bifidumbacterin forte in large doses proved that the preparation was well tolerated and could be used for the correction of interferon status.