Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of the Sugar Utilization Gene polS from Ralstonia solanacearum

The gene coding for sorbitol dehydrogenase (polS) from Ralstonia solanacearum strain TO 264 biovar 3 was cloned, sequenced, and compared to homologous sequences from the other bacteria. The result showed that the sorbitol dehydrogenase gene from R. solanacearum strain TO264 displayed the highest similarity to that of R. solanacearum GMI1000 with 99.6% amino acid similarity and the least similarity to sequence of Pseudomonas syringae pv. syringae with 56% amino acid similarity. Phylogenetic analysis of polS showed the Burkholderia cepacia sequence to join as sister to the R. solanacearum TO264 pair. Analysis of the deduced amino acid sequence revealed homology to enzymes of the short-chain dehydrogenase/reductase protein family. The eight amino acid residues were conserved in most of these proteins. These residues included the almost invariant tyrosine (Y) and lysine (K) residues of consensus sequence Y-X-X-X-K, which were essential for catalysis and were located in the active site in C-terminal whereas the glycine (G) residues of the G-X-X-X-G-X-G segment were characteristic of the NAD + binding domain in the N-terminal region. The 771 bp of polS gene from R. solanacearum TO264 was subcloned into expression pGEX-2T vector. The polS ORF encoded a protein consisting 256 amino acid residues with estimated molecular mass of 27 kDa by SDS-PAGE analysis.
Transduction pathways implicated in osteoblastic cells activation induced by parathyroid hormone (PTH)

Le recepteur a l'hormone parathyroidienne (PTH1R) est couple aux proteines G et sa stimulation induit les cascades de signalisation Gαs/AMPc/PKA et Gαq/PLCβ/PKC. La liaison de la β-arrestine au recepteur engendre sa desensibilisation et son internalisation. Le PTH1R active egalement les voies des MAPKs. Nous avons etudie les determinants structurels du recepteur et les molecules impliquees dans l'activation d'ERK1/2, ainsi que le role des MAPKs dans la differenciation osteoblastique. Nous montrons que l'activation d'ERK1/2 implique deux voies de signalisation. La premiere impliquant la liaison de Gαq a la 2eme boucle intracellulaire du recepteur. La seconde impliquant la liaison de la β-arrestine au C-proximale du recepteur, ainsi que de c-Src aux motifs polyprolines de la partie C-distale du recepteur. La deuxieme etude montre que la PTH active ERK1/2 et p38 dans les osteoblastes. L'activation de p38 depend de l'AMPc/PKA et p38 est implique dans la differenciation osteoblastique et la mineralisation.
Particle swarm optimization based networked control system design with uncertainty

This study discusses the design of state feedback control for networked control system (NCS) with uncertainty, where the feedback controller is used to solve the latency and the packet dropout caused by NCS. The conventional design approach generally adopts Lyapunov stability theorem to analyse the stability of NCSs and then Lyapunov-Krasovskii stability criterion is solved to obtain the control gain matrix. Rather than using complex Lyapunov-Krasovskii stability criterion and solving difficult linear matrix inequality (LMI) problem in the control design, this study attempts to utilize particle swarm optimization (PSO) algorithm to search an optimal feedback control gain for the NCS. Finally, the computer simulation is illustrated the proposed control strategy and demonstrated the control performance.
Indoor Abstract Spaces: Linking IndoorGML and LADM

In this paper we investigate the possible synergy between two different but related standards: OGC’s IndoorGML and ISO TC211’s LADM. Both (can) deal with 3D spaces with properties, constraints and associations attached and both can operate with abstract notations of space. But there are also differences, e.g. LADM is a conceptual model, while IndoorGML is also an actual XML schema (technical model), which can be used directly for data exchange and storage. Also, the scope is different; e.g. IndoorGML focuses on indoor spaces, while LADM addresses all spaces (in principle a complete subdivision of the countries territory, including outdoor, water and surface spaces). LADM models legal and administrative concepts such as use and ownership rights of spaces related to certain parties. IndoorGML puts emphasis on connectivity of spaces related to the navigability as one of the main use cases. These characteristics make the two standards quite complementary and this motivates our exploration in the combination of both. The spaces defined by LADM are the results of legal/administrative rights, restrictions, responsibilities (largest possible homogeneous spaces with respect to these RRRs). The space subdivision of IndoorGML is based on navigable areas and their connectivity. IndoorGML also recognizes other spaces, called abstract spaces. The paper will compare the space characteristics of the two models and will explore options to combine the models.
Looking for my lost shaker of salt... Replacer: Flavor, function, future

Salt and sodium are currently one of the top issues for healthy reformulation efforts by food and beverage manufactures on a global basis. Sodium consumption on average has increased to the point of exceeding an average daily consumption of 3,500 mg. In most countries, the source of sodium in the diet is not the salt shaker. Over 70% of the sodium in the diet is coming from processed foods, for which the consumer has little control as to the level of sodium that is present. As the cost of health care rises, with cardiovascular disease as a primary cause, health experts and leaders are looking for ways to improve the health of their nations. The spotlight, rightly or wrongly, has been placed on the reduction of sodium.
DEVELOPING OF TRIGRS (TRANSIENT RAINFALL INFILTRATION AND GRID-BASED REGIONAL SLOPE–STABILITY ANALYSIS) INTO TRIGRS MAP FOR LANDSLIDE SUSCEPTIBILITY MAPPING

ABSTRACT TRIGRS is a modeling program for slope stability against the occurrence of landslide and pore water pressure changes due to infiltration of rainfall. There are two problems in TRIGRS operation. Firstly, whole data must have no mistake before being executed in TRIGRS. Secondly, TRIGRS is not completed by spatial visualization based on Geographic Information System (GIS), so it needs GIS e.g. MapInfo, ArcGIS, and ILWIS to visualize its result. The purpose of this paper is present the result of development of TRIGRS MAP by using integrated mapping technique between MapInfo and Visual Basic. Implementation of TRIGRS MAP for Bandung Regency area has generated a landslide susceptibility map of the area. By using TRIGRS MAP, user can avoid a mistake in data initialization and directly visualize its result as a map. Thus, TRIGRS MAP can be used to process data from other area for creating the landslide susceptibility map more easily and efficiently
Vibration reduction of cable-stayed bridge model retrofitted with SMA restrainers

Shape Memory Alloys (SMAs) are more and more frequently used in civil structures subjected to extreme dynamic loadings. Due to their unique properties, the SMAs are used for seismic retrofit of bridges, tall buildings and masts. The paper presents selected results of numerical calculations of a cable-stayed steel bridge model retrofitted with SMA restrainers. The original set of constitutive relationships describing pseudoelastic properties of SMA was used for this purpose. The formulation of SMA model is based on analysis of a special rheological scheme representing its constitutive properties. The SMA model was implemented within FEM software Abaqus using an user defined subroutine. It was proved that the application of SMA restrainers can reduce significantly vibrations and internal forces caused by seismic excitations.
Using canonical correlations in testing for common nonlinear components

The paper discusses the use of canonical correlations for modelling multiple equation systems with common nonlinear components of a smooth transition type. The coefficients in a smooth transition regression model are assumed to be nonlinear continuous functions of a properly chosen transition variable. The obtained model thus captures structural changes in the relationship between the observed economic variables. With the help of the canonical correlations technique a simple test for testing for common nonlinear components helps us interpret the relationships between different economic variables and also simplifies the specification and estimation of the equation system, since in this case a reduction in the dimension of nonlinear components is possible. It was shown by Anderson and Vahid (1998) that the test for common nonlinearities is related to the test statistic for the overidentifying restrictions in the generalized method of moments framework and that both tests have the same asymptotic distribution. As an application of the described modelling approach, a three-variable linear vector autoregressive (VAR) model of the consumer price index for Slovenia and Austria and the nominal exchange rate between the currencies of both countries is investigated. A single common nonlinear component is detected in all 3 equations and the estimated multivariate logistic smooth transition autoregressive model is discussed.
Measuring the impact of knowledge loss

The impact of knowledge loss on the firm is a largely unexplored area of strategic management. This paper reports the findings of an in-depth case study of an organisation within the Australian Department of Defence. The study examines the nature and impact of knowledge loss within the construct of social capital. Of particular interest were the relationships between individuals’ activities, their levels of knowledge, and their motivation and ability to share this knowledge. The paper proposes a method to parameterise the knowledge resource variable. Findings suggest that the impact of knowledge loss may be traced directly to reduced capability in strategic activities at the organisational level, and indirectly to ineffectiveness and inefficiencies at the individual level.
"Organic Membrane-Shell" and Initial Calcification in Shell Regeneration

by Saloru Uozumi and Seiichi Suzuki (with 43 figures and 2 tables) (Contribution from the Department of Geology and Mineralogy, Faculty of Science, Hokkaido University, No. 1627) Initial mineralization of the organic membrane-shell utilized in shell regeneration in mussel was studied by electron probe microanalysis and electron microscopy. The organic membrane-shell , not-fully mineralized regenerated shell, is composed of three different layers: laminated, bI;own and conchiolin membranous layers in regeneration order. The organic matrix of the first and third are very similar, in electron microscopic and histochemical features, to the conchiolin matrix, closely associated with the mineralization of the shell. While the second may be homologous to the periostracum, outermost non-calcified layer of the mussel shell. Initial mineralization of the organic membrane-shell takes place within the conchiolin membranous layer, through the following process: Deposition of particles (nucleus of mineralization? ) -+ Aggregation of particles -+ Coating of aggregated body with amorphous organic materials (= formation of spherule) (calcium accumulation) -+ Enlargement of its size (calcium concentration and beginning of mineralization? ) -+ Transformation into double-disk body (accumulation and growth of acicular calcium carbonate crystals) -+ Exposure of preformed acicular crystals to the spaces between conchiolin membranes -+ Final formation of crystalline aggregates, structurally organized into well-ordered sheet-like layer (nacreous layer).
Kitchen Confidential? Norms for the Use of Transferred Knowledge in Gourmet Cuisine

When will knowledge holders share their knowledge with peers? Several studies suggest that norms of knowledge disclosure encourage knowledge transfer. More recently, scholars have hypothesized that norms of knowledge use may indirectly promote it. In this article, we synthesize a theoretical framework of the effect of norms of knowledge use and test its predictions by means of a field experiment involving more than 500 Italian chefs. For the literature on knowledge transfer, we confirm the importance of norms, but we also show that they are not complete substitutes for other means of protecting private knowledge. For the literature on social norms, we provide evidence of how actors assess others’ propensity to conform and how this influences the intention to participate in the norm-regulated exchange.
Two-magnon scattering and viscous Gilbert damping in ultrathin ferromagnets

Ferromagnetic resonance experiments of magnetic nanostructures over a large frequency range from $1\phantom{\rule{0.3em}{0ex}}\text{to}\phantom{\rule{0.3em}{0ex}}225\phantom{\rule{0.3em}{0ex}}\mathrm{GHz}$ are presented. We find unambiguous evidence for a nonlinear frequency dependence of the linewidth. The viscous Gilbert damping and two-magnon scattering are clearly separated. Both angular and frequency dependent measurements give a transverse scattering rate within the magnetic subsystem of the order of ${10}^{9}\phantom{\rule{0.3em}{0ex}}{\mathrm{s}}^{\ensuremath{-}1}$, whereas the longitudinal Gilbert relaxation into the thermal bath is one to two orders of magnitude smaller.
Life History Traits of the Invader Dikerogammarus villosus (Crustacea: Amphipoda) in the Moselle River, France

The latest threatening invader in European freshwaters is Dikerogammarus villosus, a large gammarid of Ponto-Caspian origin exhibiting a predatory behaviour. Its biology and population dynamics were studied over a one-year period in a recipient ecosystem to determine bio/ecological traits having facilitated its rapid establishment. The study revealed that D. villosus reaches sexual maturity early, at six mm in length, and produces three reproductive peaks, though the species reproduces all year long, hence reflecting its multivoltine character. The study also revealed a female-biased sex ratio, exceptional growth rates of up to 2.6 mm in two-weeks in spring, and one of the highest fecundities of Western Europe gammarids. D. villosus exhibits a biological profile suggesting that only a few individuals can rapidly establish a new population in a recipient ecosystem, and allow this gammarid to become cosmopolitan in the near future.
Cost benefit analysis of the night-time ventilative cooling in office building

The indoor temperature can be controlled with different levels of accuracy depending on the building and its HVAC system. The purpose of this study was to evaluate the potential productivity benefits of improved temperature control, and to apply the information for a cost-benefit analyses of night-time ventilative cooling, which is a very energy efficient method of reducing indoor daytime temperatures. We analyzed the literature relating work performance with temperature, and found a general decrement in work performance when temperatures exceeded those associated with thermal neutrality. These studies included physiological modelling, performance of various tasks in laboratory experiments and measured productivity at work in real buildings. The studies indicate an average 2% decrement in work performance per degree C temperature rise, when the temperature is above 25 C. When we use this relationship to evaluate night-time ventilative cooling, the resulting benefit to cost ratio varies from 32 to 120.
Estimation of the bottleneck size in Florida panthers

We have estimated the extent of genetic variation in museum (1890s) and contemporary (1980s) samples of Florida panthers Puma concolor coryi for both nuclear loci and mtDNA. The microsatellite heterozygosity in the contemporary sample was only 0.325 that in the museum samples although our sample size and number of loci are limited. Support for this estimate is provided by a sample of 84 microsatellite loci in contemporary Florida panthers and Idaho pumas Puma concolor hippolestes in which the contemporary Florida panther sample had only 0.442 the heterozygosity of Idaho pumas. The estimated diversities in mtDNA in the museum and contemporary samples were 0.600 and 0.000, respectively. Using a population genetics approach, we have estimated that to reduce either the microsatellite heterozygosity or the mtDNA diversity this much (in a period of c. 80 years during the 20th century when the numbers were thought to be low) that a very small bottleneck size of c. 2 for several generations and a small effective population size in other generations is necessary. Using demographic data from Yellowstone pumas, we estimated the ratio of effective to census population size to be 0.315. Using this ratio, the census population size in the Florida panthers necessary to explain the loss of microsatellite variation was c. 41 for the non‐bottleneck generations and 6.2 for the two bottleneck generations. These low bottleneck population sizes and the concomitant reduced effectiveness of selection are probably responsible for the high frequency of several detrimental traits in Florida panthers, namely undescended testicles and poor sperm quality. The recent intensive monitoring both before and after the introduction of Texas pumas in 1995 will make the recovery and genetic restoration of Florida panthers a classic study of an endangered species. Our estimates of the bottleneck size responsible for the loss of genetic variation in the Florida panther completes an unknown aspect of this account.
Positive effects of rehabilitation training with the L-EXOS in chronic stroke

This paper presents the results of the evaluation training performed in a group of chronic stroke patients with L-Exos device. The effects of training were assessed both by means of clinical evaluation in terms of Fugl-Meyer and Modified Ashworth assessment scales, and of functional evaluation, by means of Bimanual Activity Scale. Moreover at each session an automatic assessment of performance was made through the robot, and this was compared with the outcome of functional evaluation. Interestingly we found a significant improvement of both clinical and functional evaluation, and that the automatic assessment performed by the robot in terms of movement smoothness represents a strong predictor of transfer of functional ability to activity of every day life.
TERORISME DAN KEKERASAN BERLATAR BELAKANG AGAMA DI JAWA BARAT

Terorisme dan kekerasan berlatar-belakang agama yang terjadi di Indonesia erat kaitannya dengan isu-isu politik dan ekonomi domestik. Salah satu penyebabnya adalah kemiskinan. Pendapat ini sesuai dengan analisis mengenai ekonomi dan konflik yang menyatakan bahwa kondisi perekonomian yang buruk dapat meningkatkan ketidakpuasan dan kekerasan. Agresifitas seringkali dipicu oleh kesenjangan sosial-ekonomi, ketidakadilan, kemiskinan, tekanan-tekanan globalisasi selain karena tidak efektifnya manajemen publik. Dorongan untuk melakukan agresi ini juga diperkuat dengan kecenderungan orang untuk melakukan glorifikasi, atau menganggap diri dan golongan sendiri suci, serta menganggap benar ajaran agama sendiri. Kecenderungan ini melahirkan dehumanisasi dan demonisasi, yang melihat orang lain sebagai ­ bukan manusia sehingga wajar bila perlu dimanusiakan dari segi prilaku maupun pemikiran. Kelompok ini melakukan pemaksaan agar orang lain menjadi seperti diri dan kelompoknya. D emonisasi berarti pen-setanan, melihat orang lain sebagai setan. Pemahaman yang keliru terhadap ajaran agama Islam, yang antara lain menyebutkan menghalalkan pembunuhan terhadap kelompok non-Muslim juga menjadi faktor penyumbang konflik dan terorisme. Dibandingkan dengan provinsi-provinsi lainnya di Indonesia, Jawa Barat merupakan daerah asal dari banyak terjadinya terorisme dan kekerasan berbasis agama. Untuk itulah, penelitian ini difokuskan pada kota-kota di Jawa Barat, terutama yang banyak mengalami aksi-aksi kekerasan seperti Bandung, Tasikmalaya dan Bekasi. Penelitian untuk melihat faktor-faktor pendorong kekerasan berbasis agama di Jawa Barat serta efektifitas program-program pemerintah selama ini dalam mengatasi masalah ini. Kata-kata kunci: terorisme, kekerasan berbasis agama, agresivitas, agama Islam, Jawa Barat.
Lipoplex-mediated gene transfer : Influence of selected chemotherapeutic agents on transgene expression

The ultimate goal of anticancer therapies should be the elimination and/or suppression of tumour cells. Lipid-based gene transfer systems have been employed in promoting transgene expression of exogenous DNA whose products are capable of performing these roles. Since improvements in antitumour efficacy may be obtained by combining gene therapy with other therapeutic modalities such as chemotherapy, it is pertinent to characterize the influence of conventional cytotoxic drugs upon transgene expression, achieved following use of a lipoplex-mediated gene transfer system. SKOV-3 human ovarian carcinoma and B16/BL6 murine melanoma cells were used as in vitro models to evaluate levels of transgene expression. Lipoplexes, [DODAC/DOPE (1:1 mol ratio) liposomes complexed with plasmid DNA containing the luciferase or chloramphenicol acetyltransferase reporter gene] were added to cells that had been pre-exposed for 24h to various cytotoxic drugs. Transgene expression levels were established 48h post-transfection. Two in vivo models were also established to evaluate whether chemotherapeutic agents would affect transgene expression following lipoplex-mediated gene transfer. C57BL/6 mice bearing B16/BL6 tumours were injected intraperitoneally with lipoplexes, three days after receiving an intravenous drug injection equivalent to the maximum therapeutic dose. The second model analyzed transgene expression in spleen and bone marrow samples from CD1 mice. Lipoplexes were intravenously administered to mice 3,7, 14 or 21 days after they had received an intravenous drug injection equivalent to the maximum therapeutic dose. In both models, transgene expression was determined 24h post-transfection. In vitro cytotoxicity data indicates that lipoplexes did not influence the sensitivity of SKOV-3 or B16/BL6 cells to cisplatin, doxorubicin, vincristine or bleomycin. Transgene expression levels increased, decreased or remained the same (relative to lipoplex only controls), depending upon the drug, its concentration and the cell type. Combining chemotherapy with an intraperitoneal model for lipoplex-mediated gene transfer did not affect transgene expression levels evaluated in B16/BL6 tumours. The in vivo intravenous model measuring spleen and bone marrow transfection levels showed that all selected drugs caused some inhibition of transgene expression. These results suggest that DNA damage is not a mechanism by which to increase gene transfer by lipoplexes, rather drug-induced alterations in cellular uptake and processing of lipoplexes are believed to be responsible for inducing changes (increases or decreases) in transgene expression.