Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Verbal and Nonverbal Communication of Affection on Avoidantly Attached Partners’ Emotions and Message Receptiveness

Research on adult attachment in romantic relationships has focused on the negative outcomes that avoidantly attached individuals face. The present research uses observational research methods to determine if there are specific ways of communicating affection that might help avoidantly attached people reap similar levels of rewards from affectionate communication as those who are more secure. We combined three samples (Ntotal = 280 couples, 560 participants) who took turns describing a time they felt strong love for their partner, and coded their expressions for cues of verbal affection (i.e., emotion-laden words) and nonverbal affection (i.e., behavioral expressiveness). Higher levels of the speaker’s nonverbal affection were associated with stronger positive emotion and behavioral receptiveness (i.e., appearing engaged) for listeners higher in attachment avoidance. Altogether, we provide evidence that avoidantly attached individuals may experience positive outcomes from affectionate exchanges when the communication style is tailored to their unique needs.
Systemic levels of interleukin‐6 in patients with age‐related macular degeneration: a systematic review and meta‐analysis

Age‐related macular degeneration (AMD) is the most prevalent cause of irreversible vision loss in industrialized countries. Several studies have investigated systemic interleukin‐6 (IL‐6) levels of patients with AMD. In this study, we systemically reviewed the literature to provide an overview of the field and used meta‐analyses to provide a summary estimate of the standardized mean difference (SMD) of systemic IL‐6 between patients with AMD and control individuals. We searched the literature databases PubMed/MEDLINE, Embase, Web of Science and the Cochrane Central on 1 June 2019 for relevant studies on humans. Two authors independently extracted data and evaluated risk of bias. We identified 19 studies for the qualitative review with a total of more than 3586 individuals (1865 controls and 1721 with AMD). We found an overall random‐effects SMD in systemic IL‐6 levels 0.63 (95% CI: 0.28 to 0.99, p = 0.0005) corresponding to a medium effect size. In a subgroup analysis, we found that early AMD was not strongly associated with elevated IL‐6 levels (0.12, 95% CI: −0.01 to 0.24, p = 0.06), which was in contrast to the significantly elevated IL‐6 levels in patients with geographic atrophy (1.21, 95% CI: 0.41 to 2.01, p = 0.003) and patients with neovascular AMD (0.99, 95% CI: 0.34 to 1.63, p = 0.003). Our results show that the evidence today suggests an increased systemic IL‐6 in patients with AMD, but that this may be a phenomenon more closely related to the late subtypes of AMD.
Safety assessment of glutaminase from Aspergillus niger

Abstract Glutaminase (glutamine aminohydrolase EC 3.5.1.2) is used in the production of food ingredients rich in l‐glutamic acid that are added to finished foods for the purpose of enhancing or improving the savory flavor profile of food. The glutaminase enzyme preparation evaluated in these studies, designated as Sumizyme GT hereafter, is obtained by fermentation of Aspergillus niger strain GT147. The safety of Sumizyme GT was evaluated in a series of standard toxicological studies, including a 90‐day oral toxicity study in rats, an in vitro bacterial reverse mutation assay, an in vitro mammalian chromosome aberration test, and an in vivo alkaline Comet assay. Sumizyme GT was not mutagenic or genotoxic, and administration of the enzyme by gavage at doses up to 2,570 mg total organic solids (TOS)/kg body weight (bw) per day for 90 days was without any systemic toxicity. The no‐observed‐adverse‐effect level was concluded to be 2,570 mg TOS/kg bw per day, the highest dose tested. Considering that A. niger has an established history of safe use in the food industry and its safety in the production of food ingredients and food enzymes is well documented, the results of these studies provide further support of the safety of glutaminase from A. niger when used in food production.
Mutations of the gene FNIP1 associated with a syndromic autosomal recessive immunodeficiency with cardiomyopathy and pre‐excitation syndrome

AMPK (adenosine monophosphate-activated protein kinase) is phosphorylated (AMPK-P) in response to low energy through allosteric activation by Adenosine mono- or diphosphate (AMP/ADP). Folliculin (FLCN) and the FLCN-interacting proteins 1 and 2 (FNIP1, 2) modulate AMPK. FNIP1 deficiency patients have a AMPK-P gain of function phenotype with hypertrophic cardiomyopathy, Wolff-Parkinson-White pre-excitation syndrome, myopathy of skeletal muscles and combined immunodeficiency.
Effect of Multiplicity Fluctuation in Cobalt Ions on Crystal Structure, Magnetic and Electrical Properties of NdCoO3 and SmCoO3

The structural, magnetic, electrical, and dilatation properties of the rare-earth NdCoO3 and SmCoO3 cobaltites were investigated. Their comparative analysis was carried out and the effect of multiplicity fluctuations on physical properties of the studied cobaltites was considered. Correlations between the spin state change of cobalt ions and the temperature dependence anomalies of the lattice parameters, magnetic susceptibility, volume thermal expansion coefficient, and electrical resistance have been revealed. A comparison of the results with well-studied GdCoO3 allows one to single out both the general tendencies inherent in all rare-earth cobaltites taking into account the lanthanide contraction and peculiar properties of the samples containing Nd and Sm.
Like a Rolling Stone

Suddenly hearing she had breast cancer, M.A., one of the authors, a nurse with discerning witness, ponders her experience of rolling stones. At the time she worked in a center dedicated to living humanbecoming with person-family-community concerned with a cancer illness. Humanbecoming paradigm was the specific discipline guiding the care in the center. Moment-to-moment, the author (M.A.) was moving with emerging meaningful experiences of having breast cancer, shapeshifting her living quality with surprising uncertainties. The humanbecoming principles structuring meaning, configuring rhythmical patterns, and cotranscending with the possibles illustrate her story with the metaphorical expression of mountains as meaningful in a Swiss novel.
Time-Varying Kelvin Wake Model and Microwave Velocity Observation

In the synthetic aperture radar (SAR) imaging of ship-induced wakes, it is difficult to obtain the Doppler velocity of a Kelvin wake due to the lack of time-varying wake models and suitable radar equipment. The conventional Kelvin wake investigation based on the static Kelvin wake model failed to reflect time-varying characteristics, which are significant in the application of the Kelvin wake model. Therefore, a time-varying Kelvin wake model with consideration of geometric time-varying characteristics and the hydrodynamic equation is proposed in this paper, which reflects the wake’s time-varying change lacking in the conventional Kelvin wake investigation. The Doppler velocity measurement, measured by a specially designed radar, can be exploited to verify the time-varying model by the comparison of velocity fields. Ground-based multi-input multi-output (MIMO) millimeter wave radar imaging through the simultaneous switching of transceiver channels was used to obtain the Doppler velocity for the first time. Finally, promising results have been achieved, which are in good agreement with our proposed model in consideration of the experimental scene. The proposed time-varying model and radar equipment provide velocity measurements for the Kelvin wake observation, which contains huge application potential.
Hidradenitis suppurativa associated with pazopanib

Dear Editor, Pazopanib is a multitargeted, orally active small molecule exerting its effects through the inhibition of several tyrosine kinases, including vascular endothelial growth factor receptors (VEGFR-1, -2, -3), platelet-derived growth factor receptors (PDGFR-α and -β), fibroblast growth factor receptors (FGFR-1 and -3), and cytokine receptor (cKIT). It is approved by the Food and Drug Administration for treatment of advanced renal cell carcinoma and advanced soft tissue sarcoma. The most common adverse events are diarrhea, hypertension, hair color changes, nausea, anorexia, and vomiting. To the best of our knowledge, hidradenitis suppurativa has never been reported in the English-language literature associated with pazopanib. A 54-year-old Caucasian man diagnosed with renal cell carcinoma stage T2aN0M0 was successfully treated with radical nephrectomy in 2014. One year later, he had local recurrence, and computed tomography showed metastases in the lung. Pazopanib was started as first-line therapy (400 mg oral daily dose). Erythematous, violaceous papules and pustules on the mons pubis were documented a few weeks after commencing the drug. The lesions were first described as papular eruption due to pazopanib. Topical clindamycin therapy was begun. After 3 months, new lesions appeared in the anal area in addition to the inguinal area (Figure 1). A punch biopsy was performed with differential diagnosis of folliculitis and hidradenitis suppurativa. Histopathological examination was consistent with hidradenitis suppurativa. There were no lesions at other locations besides the anal and inguinal areas. Since the development of the new lesions and the complaint of the patient, 600 mg of oral clindamycin and 600 mg of rifampicin daily were started. This treatment was followed by marked improvement in his hidradenitis suppurativa, and antibiotics were stopped after 12 weeks. Rare cutaneous eruptions with pazopanib were profound in hair and skin hypopigmentation. The mechanism was believed to be the combination of c-kit and PDGF inhibition. The clear temporal association between the development of hidradenitis suppurativa (HS) lesions upon pazopanib initiation suggests that our patient's HS was triggered by pazopanib. Classic HS within the skin folds of the groin and anal area has not been previously associated with pazopanib. The mechanisms behind sorafenib-induced cutaneous toxicities remain unclear but have been associated with blockade of PDGF and KIT, the receptor for stem cell factors in the eccrine gland epithelium and antagonism of VEGFRs in subcutaneous vessels, which may lead to impaired cutaneous regeneration and healing. Hyperkeratinization of the follicle infundibulum is believed to play a central role. Secukinumab, Sorafenib, and FOLFOX chemotherapy has been associated with the development of HS in the literature. To our knowledge, this is first description of pazopanib-associated HS in the inguinal and anal areas. We encourage clinicians to be aware of the potential correlation between pazopanib and HS and recommend further studies to explore this association.
Efficacy of Antibiotic Combinations against Multidrug-Resistant Pseudomonas aeruginosa in Automated Time-Lapse Microscopy and Static Time-Kill Experiments

Antibiotic combination therapy is used for severe infections caused by multidrug-resistant (MDR) Gram-negative bacteria, yet data regarding which combinations are most effective are lacking. This study aimed to evaluate the in vitro efficacy of polymyxin B in combination with 13 other antibiotics against four clinical strains of MDR Pseudomonas aeruginosa. We evaluated the interactions of polymyxin B in combination with amikacin, aztreonam, cefepime, chloramphenicol, ciprofloxacin, fosfomycin, linezolid, meropenem, minocycline, rifampin, temocillin, thiamphenicol, or trimethoprim by automated time-lapse microscopy using predefined cutoff values indicating inhibition of growth (≤106 CFU/ml) at 24 h. ABSTRACT Antibiotic combination therapy is used for severe infections caused by multidrug-resistant (MDR) Gram-negative bacteria, yet data regarding which combinations are most effective are lacking. This study aimed to evaluate the in vitro efficacy of polymyxin B in combination with 13 other antibiotics against four clinical strains of MDR Pseudomonas aeruginosa. We evaluated the interactions of polymyxin B in combination with amikacin, aztreonam, cefepime, chloramphenicol, ciprofloxacin, fosfomycin, linezolid, meropenem, minocycline, rifampin, temocillin, thiamphenicol, or trimethoprim by automated time-lapse microscopy using predefined cutoff values indicating inhibition of growth (≤106 CFU/ml) at 24 h. Promising combinations were subsequently evaluated in static time-kill experiments. All strains were intermediate or resistant to polymyxin B, antipseudomonal β-lactams, ciprofloxacin, and amikacin. Genes encoding β-lactamases (e.g., blaPAO and blaOXA-50) and mutations associated with permeability and efflux were detected in all strains. In the time-lapse microscopy experiments, positive interactions were found with 39 of 52 antibiotic combination/bacterial strain setups. Enhanced activity was found against all four strains with polymyxin B used in combination with aztreonam, cefepime, fosfomycin, minocycline, thiamphenicol, and trimethoprim. Time-kill experiments showed additive or synergistic activity with 27 of the 39 tested polymyxin B combinations, most frequently with aztreonam, cefepime, and meropenem. Positive interactions were frequently found with the tested combinations, against strains that harbored several resistance mechanisms to the single drugs, and with antibiotics that are normally not active against P. aeruginosa. Further study is needed to explore the clinical utility of these combinations.
GPD1 Enhances the Anticancer Effects of Metformin by Synergistically Increasing Total Cellular Glycerol-3-Phosphate

GPD1 overexpression enhances the anticancer effect of metformin through synergistic inhibition of mitochondrial function, thereby providing new insight into metformin-mediated cancer therapy. Metformin is an oral drug widely used for the treatment of type 2 diabetes mellitus. Numerous studies have demonstrated the value of metformin in cancer treatment. However, for metformin to elicit effects on cancer often requires a high dosage, and any underlying mechanism for how to improve its inhibitory effects remains unknown. Here, we found that low mRNA expression of glycerol-3-phosphate dehydrogenase 1 (GPD1) may predict a poor response to metformin treatment in 15 cancer cell lines. In vitro and in vivo, metformin treatment alone significantly suppressed cancer cell proliferation, a phenotype enhanced by GPD1 overexpression. Total cellular glycerol-3-phosphate concentration was significantly increased by the combination of GPD1 overexpression and metformin treatment, which suppressed cancer growth via inhibition of mitochondrial function. Eventually, increased reactive oxygen species and mitochondrial structural damage was observed in GPD1-overexpressing cell lines treated with metformin, which may contribute to cell death. In summary, this study demonstrates that GPD1 overexpression enhances the anticancer activity of metformin and that patients with increased GPD1 expression in tumor cells may respond better to metformin therapy. Significance: GPD1 overexpression enhances the anticancer effect of metformin through synergistic inhibition of mitochondrial function, thereby providing new insight into metformin-mediated cancer therapy.
Dicer’s Helicase Domain: A Meeting Place for Regulatory Proteins

The function of Dicer’s helicase domain has been enigmatic since its discovery. Why do only some Dicers require ATP, despite a high degree of sequence conservation in their helicase domains? We discuss evolutionary considerations based on differences between vertebrate and invertebrate antiviral defense, and how the helicase domain has been co-opted in extant organisms as the binding site for accessory proteins. Many accessory proteins are double-stranded RNA binding proteins, and we propose models for how they modulate Dicer function and catalysis.
From a calf’s perspective: humpback whale nursing behavior on two US feeding grounds

Nursing influences growth rate and overall health of mammals; however, the behavior is difficult to study in wild cetaceans because it occurs below the surface and can thus be misidentified from surface observations. Nursing has been observed in humpback whales on the breeding and calving grounds, but the behavior remains unstudied on the feeding grounds. We instrumented three dependent calves (four total deployments) with combined video and 3D-accelerometer data loggers (CATS) on two United States feeding grounds to document nursing events. Two associated mothers were also tagged to determine if behavior diagnostic of nursing was evident in the mother’s movement. Animal-borne video was manually analyzed and the average duration of successful nursing events was 23 s (±7 sd, n = 11). Nursing occurred at depths between 4.1–64.4 m (along the seafloor) and in close temporal proximity to foraging events by the mothers, but could not be predicted solely by relative positions of mother and calf. When combining all calf deployments, successful nursing was documented eleven times; totaling only 0.3% of 21.0 hours of video. During nursing events, calves had higher overall dynamic body acceleration (ODBA) and increased fluke-stroke rate (FSR) compared to non-nursing segments (Mixed effect models, ODBA: F1,107 = 13.57756, p = 0.0004, FSR: F1,107 = 32.31018, p < 0.0001). In contrast, mothers had lower ODBA and reduced FSR during nursing events compared to non-nursing segments. These data provide the first characterization of accelerometer data of humpback whale nursing confirmed by animal-borne video tags and the first analysis of nursing events on feeding grounds. This is an important step in understanding the energetic consequences of lactation while foraging.
The Influence of Typography on Algorithms that Predict the Speed and Comfort of Reading

1. The speed with which text can be read is determined in part by the spatial regularity and similarity of vertical letter strokes as assessed by the height of the first peak in the horizontal autocorrelation of the text. The height of this peak was determined for two passages in 20 fonts. The peak was unaffected by the size of the text or its content but was influenced by the font design. Sans serif fonts usually had a lower peak than serif fonts because the presence of serifs usually (but not invariably) resulted in a more even spacing of letter strokes. There were small effects of justification and font-dependent effects of font expansion and compression. 2. The visual comfort of images can be estimated from the extent to which the Fourier amplitude spectrum conforms to 1/f. Students were asked to adjust iBooks to obtain their preferred settings of font and layout. The preference was predicted by the extent to which the Fourier amplitude spectrum approximated 1/f, which in turn was jointly affected by the design of the font, its weight and the ratio of x-height to line separation. Two algorithms based on the autocorrelation and Fourier transformation of text can be usefully applied to any orthography to estimate likely speed and comfort of reading.
Geographical separation and physiology drive differentiation of microbial communities of two discrete populations of the bat Leptonycteris yerbabuenae

In this paper, we explore how two discrete and geographically separated populations of the lesser long‐nosed bat (Leptonycteris yerbabuenae)—one in central and the other in the Pacific region of Mexico—differ in their fecal microbiota composition. Considering the microbiota–host as a unity, in which extrinsic (as food availability and geography) or intrinsic factors (as physiology) play an important role in the microbiota composition, we would expect differentiation in the microbiota of two geographically separated populations. The Amplicon Sequences Variants (ASVs) of the V4 region of the 16s rRNA gene from 68 individuals were analyzed using alpha and beta diversity metrics. We obtained a total of 11 566 (ASVs). The bacterial communities in the Central and Pacific populations had a diversity of 6,939 and 4,088 ASVs, respectively, sharing a core microbiota of 539 ASVs accounting for 75% of the relative abundance, suggesting stability over evolutionary time. The Weighted UniFrac metrics tested by a PERMANOVA showed that lactating and pregnant females had significant beta diversity differences in the two populations compared with other reproductive stages. This could be a consequence of the increased energy requirements of these physiological stages, more than the variation due to geographical separation. In contrast, a positive correlation of the observed ASVs of fecal microbiota with the observed ASVs of plastids related to the diet was observed in the juveniles and adults, suggesting that in these physiological stages an extrinsic factor as the diet shapes the microbiota composition. The results provide a baseline for future studies of the microbiome in these two wild populations of the lesser long‐nosed bat, the main pollinator of the Agaves from which the beverages tequila and mezcal are made.
Ellagitannins Inhibit the Exsheathment of Haemonchus contortus and Trichostrongylus colubriformis Larvae: The Efficiency Increases Together with the Molecular Size

Worldwide, parasitic gastrointestinal nematodes continue to threaten animal health, welfare, and production in outdoor breeding systems of small ruminants. For more than 50 years, the control of these parasitic worms has relied on the use of commercial synthetic anthelmintics. However, anthelmintic resistance in worm populations is nowadays widespread and requires novel solutions. The use of tannin-rich plants has been suggested as an alternative to synthetic anthelmintics to control gastrointestinal nematodes. The majority of previous studies have focused on the activity of proanthocyanidins (syn condensed tannins), and less is known about ellagitannins. In this study, the effects of 30 structurally unique ellagitannins on the exsheathment of third-stage infective larvae were examined on Haemonchus contortus and Trichostrongylus colubriformis by the in vitro larval exsheathment inhibition assay. Ellagitannins were found to be promising natural anthelmintics as they showed direct inhibition on larval exsheathment for both nematode species. In general, ellagitannins were more efficient at inhibiting the exsheathment of H. contortus larvae than those of T. colubriformis. The efficiency of inhibition increased as the degree of oligomerization or the molecular weight of the ellagitannin increased. Otherwise, we found no other structural features of ellagitannins that significantly affected the anthelmintic activity on the third-stage infective larvae. The effective concentrations were physiologically relevant and should be achievable in the gastrointestinal tract also in in vivo conditions.