Miracle-dz/UniFAR-FTUs huggingface dataset:
Zinc is both an intracellular and extracellular regulator of KATP channel function

Extracellular Zn2+ has been identified as an activator of pancreatic KATP channels. We further examined the action of Zn2+ on recombinant KATP channels formed with the inward rectifier K+ channel subunit Kir6.2 associated with either the pancreatic/neuronal sulphonylurea receptor 1 (SUR1) subunit or the cardiac SUR2A subunit. Zn2+, applied at either the extracellular or intracellular side of the membrane appeared as a potent, reversible activator of KATP channels. External Zn2+, at micromolar concentrations, activated SUR1/Kir6.2 but induced a small inhibition of SUR2A/Kir6.2 channels. Cytosolic Zn2+ dose‐dependently stimulated both SUR1/Kir6.2 and SUR2A/Kir6.2 channels, with half‐maximal effects at 1.8 and 60 μm, respectively, but it did not affect the Kir6.2 subunit expressed alone. These observations point to an action of both external and internal Zn2+ on the SUR subunit. Effects of internal Zn2+ were not due to Zn2+ leaking out, since they were unaffected by the presence of a Zn2+ chelator on the external side. Similarly, internal chelators did not affect activation by external Zn2+. Therefore, Zn2+ is an endogenous KATP channel opener being active on both sides of the membrane, with potentially distinct sites of action located on the SUR subunit. These findings uncover a novel regulatory pathway targeting KATP channels, and suggest a new role for Zn2+ as an intracellular signalling molecule.
A formal framework to build software agents

This paper presents a micro-level design perspective of a software agent that is capable of taking part in service-centric interactions with other agents in its environment. The agent software is not built as a monolithic entity but is engineered as a set of distinct modules. The components of the agent software are formally specified using the RAISE specification language. Our formal approach to agent building provides clarity at the conceptual level and eases the process of implementation.
Applying a gender lens to employment relations: Revitalisation, resistance and risks

Employment relations is on the defensive. A gender lens provides opportunities for revitalisation through bringing in social reproduction alongside production, introducing intersectional identities alongside class, developing gendered critiques of ‘neutral’ markets and recognising the ‘doing of gender’ within the workplace. However, resistance within research and practice is evident in gender blindness, marginalisation of gender issues or preference for male interests. Three risks associated with a gender lens are identified: first, feminist critiques may be used by employers or neoliberal policymakers to deregulate employment; second, by making gender visible, gender differences may be used to legitimise gender inequalities; and third, in representing workers’ interests many pitfalls need to be navigated in steering a path between excessive fragmentation and reproducing hierarchy, whether by class, gender or race. Nevertheless, the costs of not embracing a gender perspective go beyond missed opportunities for renewal and leave employment relations at risk of further decline.
Primary progressive apraxia in Pick's disease

A 62-year-old right-handed man gradually experienced increasing difficulty with speech and manual dexterity. He had apraxia of speech, buccofacial apraxia, and complex limb apraxia as well as terminal dementia. At autopsy, focal cortical atrophy, neuronal loss, and neuropil rarefaction in the second and third cortical layers were most prominent in the left opercular, lower precentral, superior parietal, and left temporal pole. Numerous Pick bodies were diffusely present in the temporal and posterior frontal lobes and, to a lesser degree, in the superior parietal lobule. This report demonstrates an association between the distribution of Pick's pathology and several apraxic impairments. NEUROLOGY 1996;47: 467-473
RQA Correlations on Real Business Cycles Time Series

Trade cycles are complex phenomena which oscillate because of economic downturns and expansions. Recurrence quantification analysis (RQA) detects state changes without necessitating any a priori mathematical assumption and highlights hidden features of the dynamics both at equilibrium and near transition phases. This paper aims to understand some potential application of recurrence quantification analysis in detecting recessions.
Inflammatory profile of new bacterial strain exacerbations of chronic obstructive pulmonary disease.

RATIONALE Whether the airway and systemic inflammatory profile in bacterial exacerbations of chronic obstructive pulmonary disease (COPD) is distinct from nonbacterial exacerbations is unclear. Previous studies have not used molecular typing of bacterial pathogens, which is required to accurately define bacterial infection in COPD. The relationship between clinical severity and course of exacerbation and inflammation is also not fully understood. OBJECTIVES To determine if (1) systemic and airway inflammation is distinct in new bacterial strain exacerbations and (2) clinical severity and resolution of exacerbations is related to airway and systemic inflammation. METHODS In a prospective longitudinal cohort study in COPD, sputum and serum samples obtained before, at, and following exacerbations during a 2-year period were studied. MEASUREMENTS AND MAIN RESULTS Clinical information, molecular typing of bacterial pathogens, sputum IL-8, tumor necrosis factor (TNF)-alpha and neutrophil elastase, and serum C-reactive protein. From 46 patients, 177 exacerbations were grouped as new strain, preexisting strain, other pathogen, and pathogen negative. New strain exacerbations were associated with significantly greater increases from baseline in sputum TNF-alpha and neutrophil elastase, and in serum C-reactive protein compared with the other three groups. Increases in inflammatory markers were similar among the other three groups. Clinical resolution was accompanied by resolution of inflammation to preexacerbation levels, whereas persistent symptoms were paralleled by persistently elevated inflammation. Clinical exacerbation severity was significantly correlated with levels of all four markers. CONCLUSIONS Neutrophilic airway inflammation and systemic inflammation are more intense with well-defined bacterial exacerbations than with nonbacterial exacerbations. Clinical course of exacerbation and inflammation are closely linked.
Potential benefit of electronic pharmacy claims data to prevent medication history errors and resultant inpatient order errors

OBJECTIVE We sought to assess the potential of a widely available source of electronic medication data to prevent medication history errors and resultant inpatient order errors. METHODS We used admission medication history (AMH) data from a recent clinical trial that identified 1017 AMH errors and 419 resultant inpatient order errors among 194 hospital admissions of predominantly older adult patients on complex medication regimens. Among the subset of patients for whom we could access current Surescripts electronic pharmacy claims data (SEPCD), two pharmacists independently assessed error severity and our main outcome, which was whether SEPCD (1) was unrelated to the medication error; (2) probably would not have prevented the error; (3) might have prevented the error; or (4) probably would have prevented the error. RESULTS Seventy patients had both AMH errors and current, accessible SEPCD. SEPCD probably would have prevented 110 (35%) of 315 AMH errors and 46 (31%) of 147 resultant inpatient order errors. When we excluded the least severe medication errors, SEPCD probably would have prevented 99 (47%) of 209 AMH errors and 37 (61%) of 61 resultant inpatient order errors. SEPCD probably would have prevented at least one AMH error in 42 (60%) of 70 patients. CONCLUSION When current SEPCD was available for older adult patients on complex medication regimens, it had substantial potential to prevent AMH errors and resultant inpatient order errors, with greater potential to prevent more severe errors. Further study is needed to measure the benefit of SEPCD in actual use at hospital admission.
Development of phonological representations and phonological awareness in children with speech impairment.

BACKGROUND Children with speech impairment are more likely to have difficulty learning to read compared with children with typical speech development. Researchers have hypothesized that a difficulty in accessing good-quality phonological representations of words stored in the memory may constrain these children's performance on phonological awareness tasks and subsequent early reading acquisition. AIMS The study investigated the following research questions. (1) Do preschool children with moderate or severe speech impairment show persistent difficulty on tasks designed to tap underlying phonological representations? (2) What is the relationship between performance on phonological representation tasks and measures of speech production, phonological awareness and early print decoding? METHODS & PROCEDURES Utilizing a longitudinal design, the performance of nine children (aged 3.09-5.03 years at initial assessment) with moderate or severe speech impairment and of 17 children of the same age with typical speech development were assessed on three occasions over a 12-month period. Assessments included receptive-based tasks designed to tap underlying phonological representations, speech production and phonological awareness measures. OUTCOMES & RESULTS Children with speech impairment had greater difficulty judging correct and incorrect productions of words, and had difficulty in reflecting on the accuracy of newly learned non-words. Moderate correlations were observed between performance on phonological representation and phonological awareness tasks. CONCLUSIONS Poorly specified underlying phonological representations will result in difficulties during listening, speaking and phonological awareness tasks, as well as create additional challenges during the decoding of written words for some children.
Influenza in children: the German perspective

Background. Influenza is recognized as an important disease in infants and children, but epidemiologic data on influenza in children are limited. Methods. We analyzed German epidemiologic data from three sources: a population-based study in children 0 to 3 years of age [Paediatric Respiratory Infection in Germany (PRIDE) study]; a multicenter hospital-based survey of children 0 to 16 years of age (www.pid-ari.net); and a sentinel study (Deutsche Arbeitsgemeinschaft Influenza) comparing influenza activity and numbers of consultations for respiratory infections in children and adults. Results. In 1996 to 2001, the PID-ARI-net and the PRIDE studies showed that the rate of influenza-associated hospitalizations was ∼120/100 000 children (0 to 5 and 0 to 3 years of age, respectively). Children born prematurely and those with underlying cardiac or pulmonary disease were at increased risk of hospitalization. The mean duration of hospital stay was 6.3 days, which is equal to the mean length of hospitalization for respiratory syncytial virus infections in continental Europe. Few nosocomial infections were reported. The outpatient consultation rate per year for lower respiratory tract illness caused by influenza virus was 1.1/100 children. Conclusion. Direct and indirect epidemiologic evidence indicates that influenza is a significant health hazard for all children.
Lithium maintenance treatment of depression and mania in bipolar I and bipolar II disorders.

OBJECTIVE Effects of long-term lithium treatment for depressive and manic phases of type I and type II bipolar disorders were compared. METHOD Clinical research records of 317 patients with DSM-IV-defined bipolar disorder (188 with type I and 129 with type II) were analyzed for frequency and duration of affective episodes and hospitalizations before (mean = 8.38 years) versus during (mean = 6.35 years) lithium maintenance treatment. Treatment effects were also assessed by survival analysis of interepisode intervals and by multivariate regression testing for factors associated with response to treatment. RESULTS Bipolar I and bipolar II patients were ill before treatment a similar percentage of time, but the subtype distinction was supported descriptively. Lithium had superior benefits in type II patients, with significantly greater reduction of episodes per year and of the percentage of time ill. Reduction of depressive morbidity was similarly strong in both diagnostic types. During treatment, bipolar II patients had 5.9-fold longer interepisode intervals and were twice as likely as type I patients to have no new episodes. Starting lithium maintenance earlier predicted greater improvement. CONCLUSIONS Lithium maintenance yielded striking long-term reductions of depressive as well as manic morbidity in both bipolar disorder subtypes, with greater overall benefits in type II patients and with earlier treatment.
A Polymorphism in the BCL-6 Gene is Associated with Follicle Center Lymphoma

Follicle center lymphoma (FCL) accounts for approximately 40% of all non-Hodgkin's lymphomas (NHL). The genetic-environmental interactions involved in the etiology and pathogenesis of this disease are unknown. In our previous study a single nucleotide polymorphism (SNP) (397C) in the regulatory untranslated first intron region of the BCL-6 gene was found in four of the eight FCL patients but in none of the 10 healthy controls. To further evaluate the potential association between the 397C allele of the BCL-6 gene and FCL, we performed a case-control study. Genomic DNA was isolated from 85 FCL patients, from 98 control cases without a previous history of malignancy, treated at Stanford University Medical Center for non-malignant disorders and from 90 samples from the DNA Polymorphism Discovery Resource. The 397G and the 397C polymorphic alleles were identified by a PCR-RFLP method. To evaluate the possible effect of this polymorphism on gene expression, BCL-6 mRNA levels in nine FCL tumors with the 397G-G genotype and in nine FCL tumors with the 397G-C genotype were measured by quantitative real-time RT-PCR. The 397C polymorphic allele was found in 32 FCL cases (37.6%), in 20 controls (20.4%) and in 17 (18.9%) samples from the DNA Polymorphism Discovery Resource. The prevalence of the 397G-C and 397C-C genotypes was significantly higher in FCL cases than in control group (p = 0.01). No difference in BCL-6 gene expression was observed between FCL cases with 397G-G and 397G-C genotypes. The present study demonstrates a possible association between the 397C allele of the BCL-6 proto-oncogene and FCL. The similar levels of BCL-6 mRNA expression in 397G-G and in 397G-C FCL cases suggests that any possible oncogenic effect of the polymorphic allele would not simply be related to a direct effect on BCL-6 gene expression and suggests the existence of other FCL susceptibility genes that are in linkage disequilibrium with the 397C allele of the BCL-6 gene.
Effect of calcium phosphate surface coating on bone ingrowth onto porous-surfaced titanium alloy implants in rabbit tibiae.

PURPOSE The purpose of the present study was to determine whether calcium phosphate coating has a significant impact on bone ingrowth into a porous titanium implant. MATERIALS AND METHODS Porous-surfaced titanium alloy Ti-6Al-4V implants were prepared with or without the addition of a thin surface layer of calcium phosphate applied by sol-gel coating. Implants were placed into the tibiae of 16 rabbits. Implanted sites were allowed to heal for 2 weeks, after which specimens were retrieved for morphometric assessment using backscatter scanning electron microscopy. RESULTS The data collected show that there is more extensive ingrowth into the porous regions of the calcium phosphate-coated implants than into the control implants. The weighted average ingrowth for the calcium phosphate-coated implants was 2.01, whereas that for the noncoated implants was 1.49; the difference is statistically significant (P <.01). CONCLUSIONS The addition of a thin layer of calcium phosphate to these implants appears to promote a more extensive implant-to-bone interface by allowing the neck regions to become intimately ingrown with bone even after only 2 weeks of initial healing.
Asthma epidemiology: has the crisis passed?

Purpose of review Asthma is a difficult, resource-intensive disease that can result in symptoms, hospitalization and, in rare cases, death. In the past few decades, there has been an abundance of evidence showing the prevalence of asthma to be increasing in certain regions. However, reports have recently emerged suggesting that asthma prevalence has stabilized. This has led to debate among researchers whether increases in asthma prevalence have come to an end. We reviewed recent literature in search of answers to the ongoing debate on whether the asthma crisis is over. Recent findings In contrast with past reports, several recent studies have reported a stabilization of asthma prevalence. However, based on repeated cross-sectional studies, findings regarding the stabilization of asthma prevalence have been inconsistent, especially when considering the heterogeneity of the disease, which can result in a variety of patterns concerning asthma diagnosis, symptoms, and allergic sensitization. Temporal trends considering physician visits, hospitalizations, and mortality have been more consistent, with stabilizing and decreasing patterns of asthma burden in recent years. Summary Because reasons for the original increases in asthma prevalence remain unclear, an explanation for the apparent stabilization of asthma prevalence reported in some studies also remains elusive. This is compounded by the difficulty in defining asthma accurately in population studies and inconsistencies in the results of prevalence estimation among repeated cross-sectional studies. Efforts should be made to continue monitoring asthma prevalence and to begin monitoring asthma prevalence in regions where environmental and social changes are occurring.
Mutations within the rhodopsin gene in patients with autosomal dominant retinitis pigmentosa.

BACKGROUND Night blindness is an early symptom of retinitis pigmentosa. The rod photoreceptors are responsible for night vision and use rhodopsin as the photosensitive pigment. METHODS AND RESULTS We found three mutations in the human rhodopsin gene; each occurred exclusively in the affected members of some families with autosomal dominant retinitis pigmentosa. Two mutations were C-to-T transitions involving separate nucleotides of codon 347; the third was a C-to-G transversion in codon 58. Each mutation corresponded to a change in one amino acid residue in the rhodopsin molecule. None of these mutations were found in 106 unrelated normal subjects who served as controls. When the incidence of these three mutations was added to that of a previously reported mutation involving codon 23, 27 of 150 unrelated patients with autosomal dominant retinitis pigmentosa (18 percent) were found to carry one of these four defects in the rhodopsin gene. All 27 patients had abnormal rod function on monitoring of their electroretinograms. It appears that patients with the mutation involving codon 23 probably descend from a single ancestor. CONCLUSIONS In some patients with autosomal dominant retinitis pigmentosa, the disease is caused by one of a variety of mutations of the rhodopsin gene.
The gap between learning and teaching in accounting education : the Saudi Arabian experience

II Declaration . IV List of Abbreviation and Acronym . V Acknowledgments . VI Dedication . VII Table of Tables . XIII Table of Figures . XV 1 Chapter 1: Introduction and Background. 1 1.
Quality management in the public sector accounting department - (un)avoidable quality costs and unlikely financial impacts

This paper investigates the nature of quality costs and the likelihood of their having financial impacts in the public sector. An illustrative quality development case project in an accounting department of a Finnish city administration is presented. The empirical data includes answers to a qualitative project question sheet, workshop material and participative observation. Quality problems are classified according to their quality cost category and the extent to which they are avoidable. Some of the quality costs proved unavoidable and some are likely to lack financial impact, exhibiting sticky cost behaviour. The study indicates that certain prevention and appraisal costs in the public sector might not deliver the desired outcomes because of such sticky behaviour of costs. Traditional quality thinking holds that increased prevention and appraisal investments decrease failure costs. However, the extent to which these costs are avoidable has not been analysed in previous studies.
The effect of context on skill acquisition and transfer.

OBJECTIVE A major concern of occupational therapy is the identification of context characteristics that optimize performance. The purpose of this study was to examine the effect of context on skill acquisition and transfer. METHOD Forty college students without disabilities (12 men, 28 women) were randomly assigned to the task of learning to use chopsticks in either a natural or a simulated context. Each participant practiced 60 trials in an acquisition phase on 1 day and was tested on a transfer task 24 hr after the acquisition phase. Their performances in the acquisition and transfer phases were measured with the variables of success rate and reaching kinematics. RESULTS The natural context elicited significantly larger improvement of success rate in the acquisition phase and a significantly higher success rate in the transfer phase than the simulated context. No major difference was found in kinematic variables between the two contexts. CONCLUSION These results suggested the use of natural contexts to facilitate the outcome of motor skill learning.
A beam source model for scanned proton beams

A beam source model, i.e. a model for the initial phase space of the beam, for scanned proton beams has been developed. The beam source model is based on parameterized particle sources with characteristics found by fitting towards measured data per individual beam line. A specific aim for this beam source model is to make it applicable to the majority of the various proton beam systems currently available or under development, with the overall purpose to drive dose calculations in proton beam treatment planning. The proton beam phase space is characterized by an energy spectrum, radial and angular distributions and deflections for the non-modulated elementary pencil beam. The beam propagation through the scanning magnets is modelled by applying experimentally determined focal points for each scanning dimension. The radial and angular distribution parameters are deduced from measured two-dimensional fluence distributions of the elementary beam in air. The energy spectrum is extracted from a depth dose distribution for a fixed broad beam scan pattern measured in water. The impact of a multi-slab range shifter for energy modulation is calculated with an own Monte Carlo code taking multiple scattering, energy loss and straggling, non-elastic and elastic nuclear interactions in the slab assembly into account. Measurements for characterization and verification have been performed with the scanning proton beam system at The Svedberg Laboratory in Uppsala. Both in-air fluence patterns and dose points located in a water phantom were used. For verification, dose-in-water was calculated with the Monte Carlo code GEANT 3.21 instead of using a clinical dose engine with approximations of its own. For a set of four individual pencil beams, both with the full energy and range shifted, 96.5% (99.8%) of the tested dose points satisfied the 1%/1 mm (2%/2 mm) gamma criterion.