Miracle-dz/UniFAR-FTUs huggingface dataset:
Binding activities of cis‐platin‐damage‐recognition proteins in human tumour cell lines

Proteins can be detected by South‐western analyses of human tumour‐cell extracts binding to double‐stranded oligonucleotide DNA treated in vitro with the chemotherapeutic drug cis‐diamminedichloroplatinum (11) (CDDP), but not to untreated DNA. The relative molecular masses of proteins binding to the CDDP‐treated double‐stranded oligonucleotide are 25, 48 and 97 kDa. The binding activity of 2 of the CDDP‐damage‐recognition proteins, of relative molecular mass 48 and 97 kDa, is greater in a CDDP‐resistant human ovarian tumour cell line than in the parental sensitive line. South‐western analysis of panel of human bladder cell lines and CDDP‐sensitive testicular cell lines show consistent patterns of CDDP‐damage‐recognition proteins within each cell type, however with differences between the 2 cell types. Binding of the proteins to CDDP‐damaged DNA and the altered binding activity detected in tumour cell lines suggests that alteration in damage‐recognition proteins could play a role in tumour response to CDDP.
Peri-operative troponin monitoring using a prototype high-sensitivity cardiac troponin I (hs-cTnI) assay: comparisons with hs-cTnT and contemporary cTnI assays

Background Non-cardiac surgery is associated with major vascular complications and higher incidences of elevated plasma troponin (cTn) concentration. Goal-directed therapy (GDT) is a stroke volume (SV)-guided approach to intravenous (IV) fluid therapy that improves tissue perfusion, oxygenation and reduces post-operative complications. In patients undergoing major gastro-intestinal surgery, we compared high sensitive and contemporary troponin assays and correlated results with patient outcome. Methods Patients (n = 135) were randomized to receive IV fluid, guided by either the central venous pressure (CVP group, n = 45) or SV (± dopexamine inotrope, n = 45 per group). Serum was obtained pre- and post-operatively (0, 8 and 24 h) for troponin analysis by a prototype hs-cTnI assay (Abbott Laboratories), hs-cTnT (Roche Diagnostics) and contemporary cTnI (Beckman Coulter) assays. Results All troponin measurements were increased (P ≤ 0.05) post-operatively but there was no difference (P > 0.05) amongst treatments. Post-operative increases were reported more frequently (P ≤ 0.05) and earlier with hs-cTnI. Temporal increases (P ≤ 0.05) were reported in patients with and without complications for hs-cTnI/T assays but only in the complications group for cTnI measurements. Elevations ≥99th centile occurred most often (P ≤ 0.05) for hs-cTnT measurements but with similar frequency for both outcome groups (all assays). Only the hs-cTnI assay showed an increased relative risk of mortality (P ≤ 0.05) for elevations ≥99th centile Conclusions Our study may suggest a possible preference for the hs-cTnI assay in the peri-operative setting; however, our findings should be verified for larger cohort studies where emerging reference range data is incorporated for improving risk prediction with hs-cTn assays.
Haploinsufficiency of Pkd2 is associated with increased tubular cell proliferation and interstitial fibrosis in two murine Pkd2 models.

BACKGROUND Autosomal dominant polycystic kidney disease (ADPKD) is the most common inherited human kidney disease and is caused by germline mutations in PKD1 (85%) or PKD2 (15%). It has been estimated that around 1% of tubular cells give rise to cysts, and cell hyperproliferation has been noted to be a cardinal feature of cystic epithelium. Nevertheless, it is uncertain whether the increase in proliferative index observed is an early or late feature of the cystic ADPKD kidney. METHODS Two Pkd2 mouse mutants (WS25 and WS183) have been recently generated as orthologous models of PKD2. To determine the effect of Pkd2 dosage on cell proliferation, cyst formation and renal fibrosis, we studied renal tissue from Pkd2(WS25/WS25) and Pkd2(+/-) mice by histological analysis. We also examined the proliferative index in archival nephrectomy tissue obtained from patients with ADPKD and normal controls. RESULTS The proliferative index of non-cystic tubules in Pkd2 mutant mice as assessed by proliferating cell nuclear antigen and Ki67-positive nuclei was between 1-2%, values 5-10 times higher than control tissue. Similarly, the proliferative index of non-cystic tubules in human ADPKD kidneys was 40 times higher than corresponding controls. In Pkd2 mutant mice, significant correlations were found between the fibrosis score and the mean cyst area as well as with the proliferative index. Of significance, proliferating tubular cells were uniformly positive for polycystin-2 expression in Pkd2(+/-) kidney. CONCLUSION These results suggest that an increase in cell proliferation is an early event preceding cyst formation and can result from haploinsufficiency at Pkd2. The possible pathogenic link between tubular cell proliferation, interstitial fibrosis and cyst formation is discussed.
Hotel Prices and Customer Perception of Rate Fairness: Analytical Study Applied to Five Star Hotels in Cairo

Recent studies have shown that online travel purchasers tend to be price driven, it is claimed that among ten travelers, more than half of them seeking the lowest prices of services and most buyers are pulled by online discounts to buy travel services. Furthermore the model of price discrimination or differential pricing provides an important analytical insight into discriminatory pricing practices. According to that model, prices charged by hotels are not usually proportional to the marginal costs incurred in producing the service. The hotels adjust their prices according to customer, location or product, and purchase volume…. etc. Many hotels are reluctant to implement differential pricing because of potential customer backlash. If customers believe that increased prices are not based on cost increases or changes in market conditions, they may view it as unfair practices. Therefore, this study focuses on answering the question of how a hotel can charge different prices without risking customer perceptions of unfairness. The study uses a questionnaire form to collect research data from the customers of the five star hotels in Cairo. A total number of 120 usable responses were collected. Linear regression was used to test the causal relationships between variables.
An Efficient Approach to Detecting the Outliers in Forest Cover Data Streams

An important challenge in network management and intrusion detection is the problem of data stream classification. Concept-evolution is the open research issue in these circumstances, which involves the occurrence of a new class in the data stream. Most traditional data stream classification techniques are based on the assumption that the number of classes does not change over time. However, that is not the case in real world networks, and existing methods generally do not have the ability of identifying the evolution of a new class in the data stream. In this paper, we present an efficient approach for the detection of novel classes in data streams that exhibit concept-evolution. In particular, our approach is able to improve both accuracy and computational efficiency by eliminating "noise" clusters and false alarm rate in the analysis of concept evolution. Through an evaluation on simulated data sets, named as forest cover type, we express that our approach achieves comparable accuracy to an existing scheme from the literature with a significant decrease in computational complexity.
Dominant Shareholders and Allied Directors: A Simple Model and Evidence from 22 Countries

We present a simple model in which a dominant shareholder chooses a board composed of independent and allied directors. We test predictions of the model with firms from 22 countries. Consistent with the model, firms have more directors allied with the dominant shareholder in countries with weaker legal protection for minority shareholders and when profitability of the firm is lower, need for external equity financing is higher, the dominant shareholder owns a higher fraction of the firm's voting rights, and a lower fraction of its cash flow rights. Further, a higher fraction of allied directors is associated with lower firm value.
Access Rights Management for the Financial Services Sector

26 Managing access to resources (data) is complicated because internal systems multiply and acquisitions 27 add to the complexity of an organization’s IT infrastructure. Identity and access management (IdAM) is 28 the set of technology, policies, and processes that are used to manage access to resources. Access rights 29 management (ARM) is the subset of those technologies, policies, and processes that manage the rights 30 of individuals and systems to access resources (data). In other words, an ARM system enables a 31 company to give the right person the right access to the right resources at the right time. The goal of this 32 project is to demonstrate an ARM solution that is a standards-based technical approach to coordinating 33 and automating updates to and improving the security of the repositories (directories) that maintain the 34 user access information across an organization. The coordination improves cybersecurity by ensuring 35
On the variability of the Priestley‐Taylor coefficient over water bodies

Deviations in the Priestley‐Taylor (PT) coefficient αPT from its accepted 1.26 value are analyzed over large lakes, reservoirs, and wetlands where stomatal or soil controls are minimal or absent. The data sets feature wide variations in water body sizes and climatic conditions. Neither surface temperature nor sensible heat flux variations alone, which proved successful in characterizing αPT variations over some crops, explain measured deviations in αPT over water. It is shown that the relative transport efficiency of turbulent heat and water vapor is key to explaining variations in αPT over water surfaces, thereby offering a new perspective over the concept of minimal advection or entrainment introduced by PT. Methods that allow the determination of αPT based on low‐frequency sampling (i.e., 0.1 Hz) are then developed and tested, which are usable with standard meteorological sensors that filter some but not all turbulent fluctuations. Using approximations to the Gram determinant inequality, the relative transport efficiency is derived as a function of the correlation coefficient between temperature and water vapor concentration fluctuations (RTq). The proposed approach reasonably explains the measured deviations from the conventional αPT = 1.26 value even when RTq is determined from air temperature and water vapor concentration time series that are Gaussian‐filtered and subsampled to a cutoff frequency of 0.1 Hz. Because over water bodies, RTq deviations from unity are often associated with advection and/or entrainment, linkages between αPT and RTq offer both a diagnostic approach to assess their significance and a prognostic approach to correct the 1.26 value when using routine meteorological measurements of temperature and humidity.
The thickness of the ligamentum flavum in relation to age and gender

Hypertrophy of the ligamentum flavum (LF) can reduce the diameter of the spinal canal posteriorly. Such stenosis may significantly compress the dural sac and nerve roots, resulting in symptoms, even without a bulging anulus fibrosus or herniated nucleus pulposus. We conducted an anatomical study to determine the influence of age and gender on the thickness of the LF at the lower lumbar levels using magnetic resonance imaging (MRI). The thickness of 1,280 ligaments was determined at the L4‐L5 and L5‐S1 levels from MRIs. We screened 320 patients (152 males [47.5%] and 168 females [52.5%]) between 21 and 82 years of age. There were no significant differences in LF thickness with respect to gender (P > 0.05). Age was not correlated with the thicknesses of the LF. The left LF at the L4‐L5 and L5‐S1 levels was significantly thicker than on the right side (P < 0.05). Furthermore, the LF thicknesses at L5‐S1 bilaterally were significantly greater than on the corresponding sides at L4‐L5 (P < 0.05). The LF is an important anatomical structure, which might cause low back or leg pain. Therefore, the thickness of the LF should be measured and evaluated carefully in the case of spinal stenosis. Clin. Anat. 23:79–83, 2010. © 2009 Wiley‐Liss, Inc.
Nervous control of the phagocytic system.

The complexity of the immune response, including numerous functional changes (thermoregulatory, circulatory, respiratory, metabolic etc.) led us (Benetato, Baciu & Vlad, 1945) to hypothesize nervous hypothalamic control of this important homeostatic response. This work synthesizes the experimental results obtained in the last four decades by us on the nervous control of the phagocytic system. Unfortunately at that time those findings were uninteresting, immunity being satisfactorily explained as a purely cellular process. The phagocytic response of the mobile (neutrophils and monocyte) system to i.v. administration of bacteria, may be reproduced by direct electrical stimulation of the hypothalamic tuberomammillary area, and by electroshock. It is blocked after sectioning the spinal cord, or after barbiturates. Using the original "isolated head" technique, applied to dogs, it was demonstrated that the injection of Salmonella typhi murium suspension in the head circulation, may activate phagocytosis in the body, isolated humorally, only through nervous effector ways, which are conserved. The current explanation is that the bacteria in the common circulation of head activates macrophages, producing the endogenous pirogen factor (II 1), which through area hypothalamica anterior acts on the tuberomammillary zone, triggering the phagocytic response by neural effector pathways. Phagocytosis stimulating substances appeared in blood, some as a result of activation of protease systems of the blood (coagulation, fibrinolysis, kininforming and complement). Epinephrine activates peripherally the fibrinolytic and kinin forming systems. Neuroimmunomodulatory effects were also demonstrated for the RES.
OD Interventions for Employer Branding: An Roi-Based Approach

This paper explores how Organization Development (OD) interventions can be used to live up to the expectations built up by organizations as part of their 'employer branding' exercises. Employer branding represents an organization's strategy to distinguish itself in the eyes of stakeholders and build up its brand image for potential recruits and existing workforce. These papers attempts to examine how astute employers can embrace measurable OD interventions and utilize the same to their benefit as a strong foundation to stand for the employer's promise.
Promoting Liquidity in Domestic Bond Markets

Domestic bond markets – by which I mean the markets for domestic debt securities issued by residents of a country in its own domestic currency – have experienced impressive growth since the late 1990s, especially in emerging market economies. Yet this growth has not been accompanied by a commensurate increase in market liquidity. Today I will first discuss some of the reasons for this lack of liquidity. Then I want to draw upon the recent experience of various countries to offer a few practical ideas for improving this situation. I shall conclude with some remarks about the Asian Bond Fund 2, which I see as an important recent initiative to reduce impediments and boost liquidity in the emerging domestic bond markets of Asia.
Mobile Phone buying behavior of consumers; A comparative study of Rural and Urban consumers in Pakistan

The purpose of this paper is to investigate the buying behavior of consumers in Pakistan and compare the consumersâ€™ behavior of urban consumers and rural consumers in Pakistan. The data are collected from 600 consumers from all over the Pakistan through a structured questionnaire. SPSS 17 is used to analyze the results. To check consumer preferences while making a purchase decision we used AHP (analytic hierarchy process). We prioritize factors through AHP. This describes better results. Our findings indicate that rural consumers more focus on the functions of mobile phone and they are price conscious on the other hand urban consumers more focus on style of mobile phone and brand image of mobile phone. This paper serves as a valuable guideline for management to review their advertisement campaigns and modify their mobiles according to the needs of consumers.
Modelo del control de nivel y caudal de líquido en un depósito cilíndrico usando la herramienta Simulink de Matlab

El presente documento, contiene detalladamente los pasos a seguir para la consecucion del modelado del control de nivel de un liquido en un deposito de forma cilindrica, se partira desde la obtencion de las ecuaciones diferenciales que representa el comportamiento dinamico de cada una de las variables del sistema, posteriormente se aplicara la transformada de Laplace suponiendo condiciones iniciales iguales a cero, se representaran las ecuaciones en forma de sistemas lineales mediante el uso de diagramas de bloques funcionales y usando el algebra de bloques se determinara la funcion de transferencia del sistema, se procedera entonces a simular el modelo usando la herramienta Simulink de Matlab, sometiendo el modelo a cambios en sus variables de entrada, para determinar su incidencia en el comportamiento de las variables de salida y los valores limites de la respuesta del sistema en cuanto a caudal y nivel se refiere.
Regeneration and myogenic cell proliferation correlate with taurine levels in dystrophin‐ and MyoD‐Deficient muscles

This study coupled proton magnetic resonance spectroscopy (1H‐NMR) and in situ hybridization plus autoradiography in a novel examination of different phenotypes of adult myogenesis that arise from genetic disruptions in mice. Study of muscle extracts from normal and dystrophin‐deficient mdx limb and diaphragm muscles confirmed our previous findings linking taurine and muscle regeneration at the peak of damage and repair. 1H‐NMR distinguished biochemical differences in regenerating muscles that were consistent with the extent of repair in three strains: mdxdystrophic mice; MyoD(‐/‐) mice that lack expression of the early myogenic regulatory gene MyoD; and a double‐mutant mdx:MyoD(‐/‐) strain lacking expression of both MyoD and dystrophin. We tested the hypothesis that differences in spectra according to genotype and the regeneration phenotype are related specifically to proliferation by committed myogenic precursor cells. 1H‐NMR distinguished the three mutant strains: Taurine was highest in mdx muscles, with the phenotype of most effective regeneration; lowest in MyoD(‐/‐) muscles, with the least effective formation of new muscle in repair, as reported previously; and intermediate in double‐mutant muscles, now reported to show an intermediate repair phenotype. The early and late muscle precursors (mpcs) expressing myf5 and myogenin were examined for proliferation. Eighteen percent of mdx myf5‐positive mpcs were proliferative, whereas myf5‐positive mpcs did not proliferate in regenerating muscles that lacked MyoD expression. By contrast, whereas 30% of myogenin‐positive mpcs were proliferative in mdxmuscles, almost none were proliferative in MyoD(‐/‐) muscles, and 12% were proliferative in double‐mutant muscles. Therefore, the extent of accumulated structural regeneration, taurine levels, and proliferation of late mpc (expressing myogenin) were congruent across genotypes. Proliferation by early mpc (expressing myf5) was inhibited by the lack of MyoD expression during muscle regeneration. These studies indicate the potential for 1H‐NMR monitoring of muscle status in disease, regeneration, and treatment. Anat. Rec. 252:311–324, 1998. © 1998 Wiley‐Liss, Inc.
Qué se puede aprender sobre el funcionamiento del cerebro a partir de la escucha dicótica

Se revisan una serie de estudios sobre el uso del estudio de la escucha dicotica como metodo para estudiar las interacciones entre cerebro y comportamiento, incluyendo la asimetria interhemisferica. Se presenta el metodo de la escucha dicotica desde una perspectiva historica seguido por una revision de los metodos empiricos utilizados en nuestro laboratorio. Posteriormente, se presentan diferentes metodos para validar este procedimiento en contra de las tecnicas invasivas y no invasivas. Finalmente, se termina con algunos ejemplos de aplicaciones clinicas de este metodo en pacientes con dano cerebral. El principal argumento en este documento es que esta tecnica es un metodo para estudiar la interaccion entre abajo - arriba, o estimulo - impulso, vs arriba ? abajo o instruccion- impulso. Esto lleva a una vision mas dinamica e interactiva sobre la lateralizacion cerebral respecto a la vision estatica tradicional de sugiere que el cerebro esta lateralizado unicamente por estimulos especificos y propiedades de los estimulos.
Diagnosis of Electrochemical Impedance Spectroscopy in Lithium Ion Batteries

Quan-Chao Zhuang1, Xiang-Yun Qiu1,2, Shou-Dong Xu1,2, Ying-Huai Qiang1 and Shi-Gang Sun3 1Li-ion Batteries Lab, School of Materials Science and Engineering, 2School of Chemical Engineering and Technology, China University of Mining and technology, Xuzhou 3State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China
ATP stimulation of P2X7 receptors activates three different ionic conductances on cultured mouse Schwann cells

Extracellular ATP, by acting on P2 purinergic receptors, is a potent mediator of cell‐to‐cell communication both within and between the nervous and the immune systems. We show here by patch‐clamp recording, fluorescent dye uptake and immunocytochemistry that, in cultured mouse Schwann cells, ATP activates a P2X7 receptor associated with three different ionic conductances. In control conditions, ATP activated an inward current (IATP) with a low potency (EC50, 7.2 mm). Replacing ATP either by the ATP analogue 2′,3′‐O‐(4‐benzoyl‐4‐benzoyl)‐ATP (BzATP) or by the tetraacidic form ATP4– potentiated the inward current (ATP4– EC50, 375 µm). ATP and BzATP currents were strongly reduced by periodate oxidized ATP (oATP), an antagonist of P2X7 receptors. IATP was a mixed current composed of a nonselective cationic conductance, a cationic conductance selective for K+ and an anionic conductance selective for Cl–. The activation of the K+ conductance was dependent on an influx of Ca2+, and was blocked by charybdotoxin (ChTX) and tetraethylammonium (TEA), two potent antagonists of large conductance Ca2+‐activated K+ channels (BK channels). The activation of the Cl– conductance was insensitive to Ca2+ but required the presence of K+. Total removal of K+ blocked both the Ca 2+‐activated K+ conductance and the Cl– conductance, unveiling the P2X7 nonselective cationic conductance. The P2X7 receptor was localized by immunocytochemistry using a polyclonal antibody, anti‐P2X7, whilst its expression and functionality were both detected by the uptake of Lucifer Yellow. This receptor could regulate the synthesis and the release of cytokines by Schwann cells during pathophysiological events.