Miracle-dz/UniFAR-FTUs huggingface dataset:
LncRNAs: genetic and epigenetic effects in plants

Abstract Long non-coding RNAs (lncRNAs) transcribed from the eukaryotic genome play important roles in essential biological processes, transcriptional and post-transcriptional gene regulation. LncRNAs act both in the nucleus and in the cytoplasm, mostly in association with chromatin in the nucleus. LncRNAs appear to be important regulators of gene expression, gene regulation and genome stability. This review outlines the major types of plant lncRNAs, their genetic and epigenetic effects with a focus on plant lncRNA instances, and discusses the recent advances in our understanding of their mechanism of action.
Personality Disorder as the Initial Presentation of a Complicated Endocarditis: Diagnostic Challenges in the CARDIO-PSYCHIATRY

Background: Bacterial endocarditis is a rare infectious entity with a very heterogeneous clinical spectrum; neurological manifestations are the most frequent and serious extracardiac complications of infective endocarditis. However, little is known about the psychiatric manifestations and / or sequels due to septic embolism of the infective endocarditis. Case Report: 50-year-old male, history of arterial hypertension and nephrolithiasis with recurrent urinary tract infections. He began his condition with behavioral changes characterized by marked impulsivity and instability in his interpersonal relationships, adding repetitive events of fever and nephrolithiasis requiring urological interventions and antibiotic therapy. Upon admission assessed by psychiatry because of his impulsive behavior and emotional changes with the presumptive diagnosis of borderline personality disorder. Conclusion: In the following work we describe the first case found in the literature of a psychiatric manifestation in a male patient as the initial complication of a subacute bacterial endocarditis, his diagnostic approach, clinical picture and therapeutic outcome. The early recognition of the clinical picture and the diagnostic approach allowed to establish effective antibiotic treatment; to recognize that bacterial endocarditis might present in a multifaceted way allows to suspect this disease and give treatment opportunely to reduce its burden and mortality.
Genetic diversity and geographic distribution of Bemisia tabaci and Trialeurodes vaporariorum in Costa Rica

Centro de Investigación en Biología Celular y Molecular (CIBCM), Escuela de Tecnología de Alimentos, Centro Nacional en Ciencia y Tecnología de Alimentos (CITA), Universidad de Costa Rica, San José, Costa Rica Centro de Investigación en Protección de Cultivos (CIPROC), Escuela de Agronomía, Universidad de Costa Rica, San José, Costa Rica Embrapa Vegetables, Brasília, Brazil Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora"-Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM UMA-CSIC), Estación Experimental "La Mayora", Málaga, Spain Correspondence N. Barboza, Centro de Investigación en Biología Celular y Molecular, San José 2060, Costa Rica. Email: natalia.barboza@ucr.ac. cr Funding information National Council for Scientific and Technological Development (CNPq); Consejo Nacional de Rectores-Fondo Especial para la Educación Superior (FEES), Grant/Award Number: VI-801-B1-650; Ministerio de Economía y Competitividad and Ministerio de Economía; Industria y Competitividad; European Regional Development Fund (ERDF) and the European Social Fund (ESF), Grant/ Award Number: AGL2016-75819-C2-2 The tobacco whitefly Bemisia tabaci (Gennadius) cryptic species complex and of the greenhouse whitefly Trialeurodes vaporariorum (Westwood) are extensively reported as destructive pests in vegetable crops worldwide. A survey was conducted in 2011 and 2012 to determine the occurrence and genetic diversity present in the populations of these whiteflies in the major vegetable production areas of Costa Rica. Insect samples were collected from sweet pepper (Capsicum annuum L.), tomato (Solanum lycopersicum L.), common bean (Phaseolus vulgaris L.) and weeds present in commercial crops either in open field or greenhouse conditions. PCR-RFLP analysis of mitochondrial cytochrome c oxidase subunit 1 gene (mtCOI) sequences of 621 whitefly individuals confirmed the presence of the Mediterranean (MED) type of the B. tabaci and of T. vaporariorum in most sampled regions. Also, individuals of the Middle East-Asia Minor 1 (MEAM1) type of the B. tabaci were observed in low numbers. Contingency analyses based on type of crop, geographical region, whitefly species, year of collection and production system confirmed that T. vaporariorum was the most frequent species in vegetable production areas in Costa Rica, both in greenhouses and in open fields. B. tabaci MED is likely spreading to new areas of the country, whereas B. tabaci MEAM1 was mostly absent or rarely found. Comparisons of mtCOI sequences from B. tabaci individuals revealed the presence of four B. tabaci sequence haplotypes (named MED-i, MED-ii, MEAM1-i, MEAM1-xviii) in Costa Rica, three of them identical to B. tabaci haplotypes previously reported in the Western Hemisphere and other parts of the world. Analysis of sequences of T. vaporariorum individuals revealed a more complex population with the presence of 11 haplotypes, two of which were identical to T. vaporariorum sequences reported from other countries.
Crop Rotations for Managing Soil-borne Plant Diseases

Soil-borne diseases result from the reduction of the biodiversity of soil organisms. Restoring beneficial organisms that attack, repel, or antagonize disease-causing pathogens will render a soil diseasesuppressive. Plants growing in disease-suppressive soil resist diseases much better than in soils low in biological diversity. Beneficial organisms can be added directly, or the soil environment can be made more favorable for them through use of compost and other organic amendments. Compost quality determines its effectiveness at suppressing soil-borne plant diseases. Compost quality can be determined through laboratory testing. Although crop rotation reduces the risk of many row crop and cereal diseases, it does not eliminate them. Small amounts of the disease organism may persist in the soil or crop refuse over extended periods. In addition, crop rotation does not affect disease organisms that survive on or in the seed, such as the cereal smuts. Crop rotation also does not affect disease organisms that blow in from the south, such as the cereal rusts.
Cuidados populares y evaluación de la percepción del apoyo social: binomio persona mayor y cuidadora familiar, Guapi-Cauca, 2019

El objetivo del estudio es describir cuidados populares y evaluacion de la percepcion del apoyo social: binomio persona mayor y cuidadora familiar, Guapi-Cauca Materiales y metodo: La investigacion tuvo tres etapas, primera etapa se realizo un analisis de datos secundarios a partir de la revision de estudios realizados en Guapi para determinar cuidados populares de la poblacion afrocolombiana residente. Segunda etapa, para la construccion del programa se utilizo Investigacion Accion Participativa y finalmente se realizo un estudio cuasi-experimental para medir el efecto de la aplicacion del programa sobre la percepcion del apoyo social en el binomio persona mayor y cuidadora familiar, Guapi- Cauca, Se consideraron aspectos eticos. Resultados: Programa apoyo social: binomio persona mayor y cuidadora familiar en Guapi-Cauca, culturalmente adaptado, mostro efectos estadisticamente significativos en la percepcion del apoyo social global y por dimensiones tanto en la persona mayor (Valorp 0,001), como en la cuidadora familiar (Valor-p 0,001), lo que corrobora las hipotesis alternas planteadas. Predominan las familias extensas modificadas (66%) y nucleares con persona mayor (34%). Las personas mayores analizadas presentan dependencia severa (60%), moderada (36%) y leve (4%). La mayoria de quienes cumplen el rol de cuidador en estas familias son mujeres (95.9 %), hijas (49%) que dedican mas de 8 horas al cuidado del familiar (35%). Conclusion: El programa apoyo social: binomio persona mayor y cuidadora familiar en Guapi-Cauca, culturalmente adaptado, mostro efectos estadisticamente significativos enla percepcion del apoyo social global y por dimensiones en personas mayores con discapacidad y sus cuidadoras familiares.
How traffic facilitates population expansion of invasive species along roads: The case of common ragweed in Germany

Handling Editor: Anibal Pauchard Abstract 1. Because common ragweed (Ambrosia artemisiifolia L., henceforth Ambrosia) has negative effects on human health, it is a common focus for management, which would benefit from a better understanding of the underlying mechanisms by which the species spreads. Road systems are known to be invasion corridors, but the conduit function of vehicles for the rapid spread of Ambrosia along roads and for population extension along roadside verges has not yet been demonstrated convincingly. 2. To quantify the effect of different traffic volumes on the dispersal and population extension of Ambrosia, we used two approaches: First, by combining field experiments along roads with records of the seed rain around single plants, we simulated a combined dispersal kernel that revealed the interactions between primary dispersal and traffic-mediated secondary dispersal. Second, we recorded the seedling recruitment around isolated roadside populations over 2 years to determine how traffic-related parameters affect population extension. 3. The longest traffic-mediated dispersal distances exceeded those of primary dispersal by about one order of magnitude. Traffic volume had a significant positive effect on dispersal distances and on the lateral deposition of seeds on the road verge. 4. Seedling recruitment around isolated roadside populations was significantly higher in the driving direction than against, but only at the distance where the major seed rain of traffic-mediated dispersal is to be expected according to the combined dispersal kernel (3–15 m). 5. Synthesis and applications. This study isolates the effects of road traffic from confounding mechanisms (e.g. mowing machinery, propagule pressure from infested fields) on common ragweed (Ambrosia artemisiifolia L.) invasions. Results demonstrate the traffic-mediated dispersal in Ambrosia invasions as a routine and predictable process that facilitates population extension in the direction of traffic along roadsides, depending on traffic volume. This highlights the importance of prioritizing mowing along high use roads and mowing of isolated populations to prevent seed abscission and further spread of common ragweed.
Influence of nonionic surfactant on the solubilization and biodegradation of phenanthrene

Phenanthrene was solubilized in two different nonionic surfactants, Tween80 and Triton X-100. The bioavailability of phenanthrene to the bacteria isolated from the petroleum contaminated soils was studied based on the rotary flasks experiments. The results showed that the concentration of nonionic surfactants above the critical micelle concentration(CMC) can increase the solubility of phenanthrene in water and were innoxious to the phenanthrene-degrading bacteria; phenanthrene solubilized in the micelles of Tween80 was bioavailable and biodegradable. The research demonstrated the potential of surfactant-enhanced bioremediation of soils contaminated by hydrophobic organic compounds( HOCs).
The Effect of Bupivacaine on Pain Relief After Lumbar Decompression Surgery

s Aim: there are many reports about the efficacy of analgesics in reducing postoperative pain after lumbar decompressive surgery. This study aimed to investigate the effect of subdermal and intramuscular injection of bupivacaine on the severity of postoperative pain after lumbar decompression surgery. Material and Methods: in this randomized double-blind trial, 50 patients who candidate for lumbar decompressive surgery elective for this them. Bupivacaine (0.25%) in the intervention group and normal saline (40 ml) in the control group were injected subcutaneously and intramuscularly. Factors of age, size of surgical cutting, number of operating spaces, duration of operation, the rate of need to analgesia, and the severity of pain at times of 3,12 and 24 hours after surgery were assessed. Results: the severity of pain in Bupivacaine group, in hours 3, 12 and 24 after postoperative surgery was less than control group (P value<0.05). Also, there was no significant difference between the two groups in terms of mean pain intensity using repeated measure design (P value<0.05). But within Bupivacaine group, pain severity significantly decreased during the 24 hours after surgery. (p<0.05) Conclusion: Although bupivacaine (0.25%) results in reducing pain after surgery, more studies are recommended to investigate the effect of bupivacaine in combination with other drugs in cases of lumbar decompressive surgery. Also, it reduces pain during the 24 hours after surgery is very important.
Bioavailability Improvement of Carbamazepine via Oral Administration of Modified-Release Amorphous Solid Dispersions in Rats

The purpose of this study was to improve the bioavailability of carbamazepine (CBZ), a poorly water-soluble antiepileptic drug, via modified-release amorphous solid dispersions (mr-ASD) by a thin film freezing (TFF) process. Three types of CBZ-mr-ASD with immediate-, delayed-, and controlled-release properties were successfully prepared with HPMC E3 (hydrophilic), L100-55 (enteric), and cellulose acetate (CA, lipophilic), defined as CBZ-ir-ASD, CBZ-dr-ASD, and CBZ-cr-ASD, respectively. A dry granulation method was used to prepare CBZ-mr-ASD capsule formulations. Various characterization techniques were applied to evaluate the physicochemical properties of CBZ-mr-ASD and the related capsules. The drug remained in an amorphous state when encapsulated within CBZ-mr-ASD, and the capsule formulation progress did not affect the performance of the dispersions. In dissolution tests, the preparations and the corresponding dosage forms similarly showed typical immediate-, delayed-, and controlled-release properties depending on the solubility of the polymers. Moreover, single-dose 24 h pharmacokinetic studies in rats indicated that CBZ-mr-ASD significantly enhanced the oral absorption of CBZ compared to that of crude CBZ. Increased oral absorption of CBZ was observed, especially in the CBZ-dr-ASD formulation, which showed a better pharmacokinetic profile than that of crude CBZ with 2.63- and 3.17-fold improved bioavailability of the drug and its main active metabolite carbamazepine 10,11-epoxide (CBZ-E).
An evaluation of a third-party logistics provider: The application of the rough Dombi-Hamy mean operator

Third-party logistics (3PL) has involved a significant response among researchers and practitioners in the recent decade. In the global competitive scenario, multinational companies (MNCs) not only improve the quality of the service and increase efficiency, but they also decrease costs by means of 3PL. However, the assessment and selection of 3PL is a very critical decision to make, comprising intricacy due to the existence of various imprecisely based criteria. Also, uncertainty is an unavoidable part of information in the decision-making process and its importance in the selection process is relatively high and needs to be carefully considered. Consequently, incomplete and inadequate data or information may occur among other various selection criteria, which can be termed as a multi-criteria decision-making (MCDM) problem. Rough numbers are very flexible to model this type of uncertainty occurring in MCDM problems. In this paper, the Hamy Mean (HM) operator and Dombi operations are expanded by rough numbers (RNs) so as to propose the Rough Number Dombi-Hamy Mean (RNDHM) operator. Then, the Multi-Attribute Decision-Making (MADM) model is designed with the RNDHM operator. Finally, the RNDHM is employed to achieve the final ranking of the 3PL providers.
Incorporation of Charge Transfer into Classical Molecular Dynamics Force Fields with Applications in Physical Chemistry.

The presence of charge transfer (CT) interactions is clear in a variety of systems. In CT, some electron density is shifted from one molecule to another (non-bonded) molecule. The importance of this CT interaction is unclear. Previous attempts to look at the consequences of CT required the use of ab initio molecular dynamics (AIMD), a computationally intensive method. Herein, a method for including CT in force field (FF) simulations is described. It is efficient, produces charges in agreement with AIMD, and prevents longranged CT. This CT MD method has been applied to monatomic ions in water. When solvated, ions do not have an integer charge. Anions give up some electron density to their ligands, and cations receive some electron density from their ligands. In bulk, the first solvation shell does not compensate for all CT, i.e. the charge is not smeared out over the first solvation shell. Rather, some charge is also found in the second solvation shell and further into the bulk. The charge of the first solvation shell depends on the balance between ion-water and water-water CT. When an interface is present, the charge outside of the second solvation shell will reside at the interface. This occurs even when the ion is over 15 Å away from the surface. The effect of long-ranged CT is mediated by changes in the hydrogen bonding patterns in water induced by the ions (not direct CT from the ions to distant waters). The model has also been applied to water’s ‘‘self-ions’’ hydronium and hydroxide. Trajectories from the multi-state empirical valence bond model (MS-EVB3) are analyzed. The differences between monatomic and molecular ions are explored. The direction of CT and the effect of hydrogen bonding with the ion are considered. The damping of CT as ligands are added is discussed and a method to improve the MD model, in order to account for damping, is proposed.
Expression estimation and eQTL mapping for HLA genes with a personalized pipeline

The HLA (Human Leukocyte Antigens) genes are well-documented targets of balancing selection, and variation at these loci is associated with many disease phenotypes. Variation in expression levels also influences disease susceptibility and resistance, but little information exists about the regulation and population-level patterns of expression due to the difficulty in mapping short reads to these highly polymorphic loci, and in accounting for the existence of several paralogues. We developed a computational pipeline to accurately estimate expression for HLA genes based on RNA-seq, improving both locus-level and allele-level estimates. First, reads are aligned to all known HLA sequences in order to infer HLA genotypes, then quantification of expression is carried out using a personalized index. We use simulations to show that expression estimates are not biased due to divergence from the reference genome. We applied our pipeline to GEUVADIS dataset, and compared the quantifications to those obtained with reference transcriptome, and found that a substantial portion of the variation captured by the HLA-personalized index in not captured by the standard index (23%). We describe the impact of the HLA-personalized approach on downstream analyses for seven HLA loci (HLA-A, HLA-B, HLA-C, HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRB1). Although the influence of the HLA-personalized approach is modest for eQTL mapping, the p-values and the causality of the eQTLs obtained are better than when the reference transcriptome is used. Finally, we integrate information on HLA-allele level expression with the eQTL findings to show that the HLA allele is an important layer of variation to understand HLA regulation.
Dinámicas familiares después de un accidente cerebrovascular en Cali, Colombia: un estudio fenomenológico

Este estudio cualitativo, con enfoque fenomenologico, se propuso comprender los cambios en las dinamicas familiares de cuatro familias de Cali, Colombia cuando uno de sus miembros sobrevive a un accidente cerebro vascular (ACV). Se uso un cuestionario sociodemografico y una guia de entrevista semi-estructurada. Las categorias de analisis de primer orden fueron: organizacion y estructura, creencias y recursos, a las cuales se sumaron dos categorias emergentes: perdidas ambiguas y el camino hacia la resiliencia; y fatiga del cuidador. Entre los hallazgos se encontro una reorganizacion de roles y funciones en la estructura familiar, y la emergencia de nuevas formas de vinculo. Se evidenciaron creencias asociadas al impacto del ACV, asi como al proceso de recuperacion. Las familias dieron cuenta de recursos individuales, familiares y contextuales que favorecieron el afrontamiento del ACV de manera resiliente y expresaron sentimientos contradictorios asociados a la perdida ambigua generada por el ACV, y los cuidadores principales refirieron sentimientos que afectaron su calidad de vida. Es fundamental reconocer de manera compartida las nuevas circunstancias, reorganizarse a nivel familiar, enfocarse en nuevas relaciones y metas de vida son factores que favorecen la resiliencia. Se brindan conclusiones y recomendaciones para la practica y la investigacion futura.
Sudden Sensorineural Hearing Loss After Spinal Anesthesia: A Case Report

Introduction: Hearing loss has been reported to occur after different anesthetic techniques. It is often subclinical and can easily be obscured by common symptoms such as headache, nausea and dizziness. Hearing loss after spinal anesthesia is a rare phenomenon. When it occurs, it can be unilateral or bilateral and transient or permanent. Case description: We report a case of a sudden decrease in hearing since the night of surgery, which was performed under spinal anesthesia. An audiometry showed bilateral mild-to-moderate sensorineural hearing loss, which was primarily affect low and mid-frequencies. Bed rest with hydration and oral prednisolone was administered for 5 days and tapered over 5 days. Follow-up audiogram was performed daily and the patient recovered to normal hearing within a week. Conclusion: Sudden sensorineural hearing loss after spinal anesthesia is a possible complication. Multiple etiologies have been proposed in the literature. Clinicians should be aware of this complication and provide prompt attention and treatment to patients complaining of headache, decreased hearing, and/or tinnitus after spinal anesthesia. We suggest informing patients about this rare complication.