Miracle-dz/UniFAR-FTUs huggingface dataset:
Reassociation and reactivation of yeast glyceraldehyde-3-phosphate dehydrogenase after dissociation in the presence of ATP.

Tetrameric d-glyceraldehyde-3-phophate dehydrogenase from yeast in the presence of 5 mM ATP and 0.1 M 2-mercaptoethanol at pH 8.5 can be reversibly inactivated and dissocited by changing the temperature from 25 to 0°C. Using ultracentrifugation and gel chromatography, the inactive low-temperature dissociation product is found to be a compact monomer (Mr= 36000, s20,w= 3.2S) which is still capable of binding on molecule of NAD+ per subunit. Deactivation caused by dissociation at 0°C is a slow process with a first-order rate constant of k= 0.007 min −1 (τ1/2= 180 min); it follows the instantaneous competitive inhibition of the ezyme by ATP which is characterized by Ki= 2.3 mM. Reactivation at high enzyme concentration (c > 10 μg/ml) obeys first-order kineics. At low enzyme concentration a shift to a higher reaction order is obseved. Alimiting value of n= 2 is reached at 0.9 μg/ml. The data are in accordance with a uni-bi molecular mechanism involving the association of (inactive) monomers as a prerequisite of enzymatic of enzymatic activity. The kinetic constants. k1= 9 ± 2 × 10−1 and k2= 7 ± 1 × 104 M−1× s−1, correspond to previous data reported for the reactivation after full denaturation and dissociation of the enzyme in 6 M guanidine · HCI at pH 2.3 [Rudolph et al. Eur. J. Biochem. 81, 563–570 (1977)]. This suggests a common intermediate in the reactivation process after deactivation and disscoiation under widely differing conditions.
CHAPTER 5 ibre type regionalisation in lower hindlimb muscles of rabbit , rat and mouse : a comparative study

The topographical distribution of different fibre types was quantitatively determined within muscles of the lower hindlimb in rabbits and mice. The results were compared to those previously obtained, using the same new quantification methods, in homologous muscles of the rat. “Slow” type I fibres were identified and mapped out in crosssections stained for myofibrillar ATPase (in “slow” soleus done for type II fibres). At the proximo-distal midlevel this was done for 11"fast” muscles in the rabbit and 7 “fast” muscles in the mouse. For 5 muscle species from each animal, more proximal and distal levels were also included (extensor digitorum longus; flexor digitorum and hallucis longus; gastrocnemius medialis; peroneus longus; tibialis anterior). All the investigated “fast” muscles showed a significant degree of topographical eccentricity in the midlevel distribution of type I fibres. For most muscles, the direction of this “vector regionalisation” of type I fibres was similar between the three animal species. For homologous muscles, the degree of vector regionalisation was significantly different: mouse > rat > rabbit. The relative area of the region containing the type I fibres, inversely related to the degree of “area regionalisation”, was also significantly different: mouse < rat < rabbit. Also within each animal species, muscles with a marked degree of vector regionalisation tended to show a marked area regionalisation. Proximo-distal differences in type I fibre density were observed in all the three species of animals; also these patterns showed marked inter-species differences. The findings demonstrate the general occurrence of, and systematic relationships between, different aspects of type I fibre regionalisation. The observed inter-species differences suggest that the expression of this phenomenon is adapted to differing functional needs.
Conducta suicida en la población general adulta española: un estudio epidemiológico

El interes por el estudio del suicidio se ha mantenido estable a lo largo de los anos, si bien en los ultimos tiempos ha aumentado la consideracion de factores socioeconomicos para evaluar si la crisis economica ha supuesto un aumento de las muertes por suicidio. Los principales objetivos de esta tesis fueron analizar las prevalencias de conducta suicida en la poblacion general adulta espanola y evaluar cuales son los factores asociados con dicha conducta en diferentes grupos de edad. en la literatura no abundan los trabajos que estudian conducta suicida en la poblacion general, por lo que esta tesis supone una buena oportunidad para disponer de datos representativos a nivel nacional con los que analizar posibles factores relacionados con la presencia de ideas, planes e intentos de suicidio. La introduccion de esta tesis es bastante completa, especialmente la parte dedicada a los estudios sobre conducta suicida que se han llevado a cabo en Espana. la bibliografia consta de un gran numero de referencias (todas ellas necesarias), mostrando parte del trabajo de investigacion desarrollado en el estudio.
Mechanisms of calcium antagonist-induced vasodilation.

Excitation-contraction coupling in smooth and cardiac muscle can be dis­ rupted by a number of organic compounds known collectively as Ca antago­ nists (CAts). In 1964, Fleckenstein (1) provided the first important clue regarding their possible mechanism of action when he noted that the cardiac effects of two of these agents, verapamil and prenylamine, were similar to those of Ca2+ removal from the extracellular space. A few years later, he proposed that CAts exerted their negative inotropic and coronary vasodila­ tor effects by blocking the entry of Ca2+ into the cells (2, 3). The vasodila­ tor and cardiac effects of CAts are having a great impact on cardiovascular medicine; consequently many reviews and monographs on this subject have appeared in the recent past (4--9). The purpose of this review is to provide an in-depth analysis of the mechanisms of action of CAts, rather than to compile an exhaustive survey of the literature. Only when we know how CAts interact with the processes that regulate Ca availability and sensitivity in smooth muscle can we understand why agents that "antagonize" the action of such a universal intracellular messenger can be effectively de­ ployed as selective therapeutic agents in cardiovascular medicine.
TGF-beta2 in aqueous humor suppresses S-phase entry in cultured corneal endothelial cells.

PURPOSE Corneal endothelium in vivo is arrested in G1, the phase of the cell cycle that prepares cells for DNA synthesis. In many cell types, transforming factor (TGF)-beta inhibits proliferation by inducing G1-phase arrest. Evidence indicates that corneal endothelial cells synthesize mRNA for TGF-beta1 and are also bathed in aqueous humor that contains TGF-beta2 (mainly in a latent form). As such, this cytokine may maintain the corneal endothelium in a G1-phase-arrested state in vivo. The purpose of these studies was to determine the effect of exogenous TGF-beta2 and TGF-beta2 in aqueous humor on DNA synthesis in cultured corneal endothelial cells. METHODS Rat corneal endothelial cells were grown in explant culture and identified by morphology and reverse transcription-polymerase chain reaction using primers for specific corneal cell markers. Subconfluent cells were synchronized in the G0 phase (quiescence) by serum starvation for 24 hours. Serum was then added to the cells in the presence or absence of exogenous TGF-beta2 or activated rat aqueous humor. [3H]Thymidine was added, and radioactivity was measured at various time points to detect DNA synthesis. Preincubation of exogenous TGF-beta2 or activated rat aqueous humor with neutralizing antibody was used to test for cytokine specificity. RESULTS A linear increase in [3H]thymidine incorporation began approximately 16 hours after serum addition, and peak incorporation occurred at approximately 24 hours. Exposure of cells to serum plus TGF-beta2 suppressed [3H]thymidine incorporation in a dose-dependent manner at concentrations ranging from 5 pg/ml to 5 ng/ml. [3H]Thymidine incorporation was also suppressed in cells exposed to serum plus rat aqueous humor diluted 1:10. Neutralizing antibody reversed the effects of both exogenous TGF-beta2 and aqueous humor. CONCLUSIONS Exogenous TGF-beta2 and TGF-beta2 in aqueous humor suppress S-phase entry of rat corneal endothelial cells. These results suggest that this cytokine in aqueous humor could help maintain the corneal endothelium in a G1-phase-arrested state in vivo.
Power sharing correction in angle droop controlled inverter interfaced microgrids

Power sharing between angle-droop controlled inverter-based sources largely depends on the line impedances and choice of droop coefficients. Simple power correction methods such as set-point correction and droop coefficient modification work satisfactorily for specific network topologies and require only limited amount of communication. However, their performance can be inadequate for microgrids with different topologies. In this work, we propose a topology-independent power sharing correction technique based on inter-node communications in order to eliminate the power sharing errors between inverters. We analyse stability and convergence of the proposed solution and present simulation results. Finally, we study the robustness of frequency-droop and angle-droop controlled systems with respect to unknown impedances and parameter uncertainties.
High-pressure x-ray tomography microscope: Synchrotron computed microtomography at high pressure and temperature

A new apparatus has been developed for microtomography studies under high pressure. The pressure generation mechanism is based on the concept of the widely used Drickamer anvil apparatus, with two opposed anvils compressed inside a containment ring. Modifications are made with thin aluminum alloy containment rings to allow transmission of x rays. Pressures up to 8GPa have been generated with a hydraulic load of 25T. The modified Drickamer cell is supported by thrust bearings so that the entire pressure cell can be rotated under load. Spatial resolution of the high pressure tomography apparatus has been evaluated using a sample containing vitreous carbon spheres embedded in FeS matrix, with diameters ranging from 0.01to0. 2mm. Spheres with diameters as small as 0.02mm were well resolved, with measured surface-to-volume ratios approaching theoretical values. The sample was then subject to a large shear strain field by twisting the top and bottom Drickamer anvils. Imaging analysis showed that detailed microst.
Scan Chain Ordering to Reduce Test Data for BIST-Aided Scan Test Using Compatible Scan Flip-Flops

In this paper, the scan chain ordering method for BIST-aided scan test for reducing test data and test application time is proposed. In this work, we utilize the simple LFSR without a phase shifter as PRPG and configure scan chains using the compatible set of flip-flops with considering the correlations among flip-flops in an LFSR. The method can reduce the number of inverter codes required for inverting the bits in PRPG patterns that conflict with ATPG patterns. The experimental results for some benchmark circuits are shown to present the feasibility of our test method.
Psychological well-being and health . Contributions of positive psychology

Positive Psychology is contributing to a more precise definition of the outline of human well-being and is fully incorporating studies on positive elements (strengths and positive emotions) that are without doubt amplifying the framework of research and conduct of psychology, in particular that of Clinical Psychology and Health Psychology. Over the last few years, academic debate has, from a scientific perspective, gone back to two ancient philosophical orientations, namely hedonism and eudaimonia. The hedonic approach conceives well-being as the presence of positive affect and lack of negative affect, whilst the eudaimonic perspective regards well-being as the consequence of a full psychological actualization from which people develop their whole potential. Whether assessed from a hedonic or eudaimonic perspective, well-being seems to play a role in the prevention of and in the recovery of physical conditions and diseases and so possibly contributes to an increase in life expectancy. Finally, the implications of these findings are discussed both from an academic perspective and, more generally speaking, from a social and political point of
A scoping review of environmental health nursing research

OBJECTIVE The purpose of this scoping review was to determine the extent and focus of published environmental health nursing research. DESIGN AND SAMPLE The search was limited to peer reviewed, English-language environmental health nursing research with at least one nursing author, published between 1995 and 2015 in a nursing journal, and catalogued in CINAHL. RESULTS Publication of the 548 identified articles occurred in 118 different nursing journals. Annual number of publications increased from nine articles in 1995 to 50 in 2013 and 2014. Most (63%) of the studies occurred in the Unnited States; the remaining studies took place in 33 other countries. Three primary focus areas were identified: disasters/disaster preparedness, occupational health, and the home environment. Other focus areas included environmental exposures, environmental risk perception, secondhand smoke, and environmental health education. The primary populations studied were nurses/nursing students (40%) and adults (26%). Most common research designs employed were cross-sectional (42%) and qualitative methods (20%). CONCLUSIONS Findings from this scoping review support a global expansion of published environmental health nursing research addressing a variety of environmental health topics. Additional studies are needed that use more complex research methods and address timely topics such as climate change and sustainability.
Damage to Nectarines by the Western Flower Thrips (Thysanoptera: Thripidae) in the Interior of British Columbia, Canada

Abstract The phenology of damage by the western flower thrips, Frankliniella occidentalis (Pergande), on nectarines was investigated using sticky cards and direct sampling of buds between 1993 and 1995 in the interior of British Columbia, the most susceptible period for damage by western flower thrips to nectarines. The life stage responsible for damage and variation in susceptibility to damage of 11 different nectarine varieties were determined. To evaluate the predictive ability of 2 sampling methods, thrips were counted from both buds and sticky cards before petal fall and correlated to damage levels at husk drop. Damage to nectarines was caused almost entirely by larval feeding at petal fall. No predictive relationships between adult or larval densities of western flower thrips and subsequent damage to fruit were apparent. Varieties did not differ in terms of larval densities at petal fall or the subsequent damage to fruit. Female western flower thrips oviposit in nectarine buds from dormant through bloom stages primarily in sepal tissues in the early buds, and in filaments and petals as these become available.
Complete Genome Sequence of Bordetella pertussis Strain VA-190 Isolated from a Vaccinated 10-Year-Old Patient with Whooping Cough

ABSTRACT The number of cases of pertussis has increased in the United States despite vaccination. We present the genome of an isolate of Bordetella pertussis from a vaccinated patient from Virginia. The genome was sequenced by long-read methodology and compared to that of a clinical isolate used for laboratory studies, D420.
Market Orientation as a Strategic Driver of Individual Customer Orientation

The main objective of this study is to investigate the effect of market orientation on individual customer orientation through a theoretical framework. This study also examines the outcomes of individual customer orientation. The suggested theoretical framework was tested using the data obtained from a random sample of marketers from a wide spectrum of businesses. The final sample consisted of 189 usable responses. No nonresponse bias was found since there were no differences between the mean responses of the first and the last quartiles (Armstrong and Overton 1977). Principal component analysis was conducted on each construct of the model to verify a single factor structure. The reliability of each construct was higher than the cutoff value of 0.70 recommended by Nunnally (1978). A confirmatory factor analysis (Joreskog and Sorbom 1993) was used to estimate the model parameters, to assess the model fit, and to test the suggested hypotheses. The fit between the suggested model and the sample data was found to be very good. According to the study results, there is a significant positive relationship between market orientation and customer orientation. The study results suggest that higher levels of customer orientation lead to higher levels of relationship development and individual performance. Managerial implications of the study results were also discussed and future research suggestions were provided.
Applying the erythrocyte Pig‐a assay concept to rat epididymal sperm for germ cell mutagenicity evaluation

The Pig‐a assay, a recently developed in vivo somatic gene mutation assay, is based on the identification of mutant erythrocytes that have an altered repertoire of glycosylphosphatidylinositol (GPI)‐anchored cell surface markers. We hypothesized that the erythrocyte Pig‐a assay concept could be applied to rat cauda epididymal spermatozoa (sperm) for germ cell mutagenicity evaluation. We used GPI‐anchored CD59 as the Pig‐a mutation marker and examined the frequency of CD59‐negative sperm using flow cytometry. A reconstruction experiment that spiked un‐labeled sperm (mutant–mimic) into labeled sperm at specific ratios yielded good agreement between the detected and expected frequencies of mutant–mimic sperm, demonstrating the analytical ability for CD59‐negative sperm detection. Furthermore, this methodology was assessed in F344/DuCrl rats administered N‐ethyl‐N‐nitrosourea (ENU), a prototypical mutagen, or clofibrate, a lipid‐lowering drug. Rats treated with 1, 10, or 20 mg/kg body weight/day (mkd) ENU via daily oral garage for five consecutive days showed a dose‐dependent increase in the frequency of CD59‐negative sperm on study day 63 (i.e., 58 days after the last ENU dose). This ENU dosing regimen also increased the frequency of CD59‐negative erythrocytes. In rats treated with 300 mkd clofibrate via daily oral garage for consecutive 28 days, no treatment‐related changes were detected in the frequency of CD59‐negative sperm on study day 85 (i.e., 57 days after the last dose) or in the frequency of CD59‐negative erythrocytes on study day 29. In conclusion, these data suggest that the epidiymal sperm Pig‐a assay in rats is a promising method for evaluating germ cell mutagenicity. Environ. Mol. Mutagen. 58:485–493, 2017. © 2017 Wiley Periodicals, Inc.
Evolution of Insulin Secretory Response to Glucose by Perifused Islets From Lean (FA/FA) Rats Chronically Infused With Glucose

Chronic infusion of nondiabetic rats with glucose for up to 7 days modified the insulin secretory response by subsequently perifused islets. Thus, on the 1st day of infusion with 40% glucose, the islets responded to 16.7 mM glucose with a 3.9-fold increase in insulin release during the first 10 min with no significant change during the second-phase insulin output compared with the control group. On the 2nd day, there was a 2-fold enhancement of insulin release during the initial 10 min of stimulation, the second phase being similar to control islets. On the 5th day of infusion, the pattern of insulin release was not significantly different from the control group. After 7 days of infusion, there was a 46% decrease in first-phase and a 33% decrease in second-phase insulin response to glucose. The response to 10 mM arginine plus 5 mM glucose was not modified by chronic glucose infusion. Priming experiments indicate that islets from rats infused for 7 days were not able to recover the normal pattern of secretion in vitro. Islets from 7-day glucose-infused rats contained 75% more protein and had a significantly higher insulin content than control islets, suggesting that insulin synthesis is not involved in the loss of the response observed. Glucose metabolism by the islets was modified by glucose infusion with a significant increase in glucose utilization and no changes in glucose oxidation, suggesting that alterations in mitochondrial oxidative events are involved in the phenomenon of desensitization.
The R*-Tree: An Efficient and Robust Access Method for Points and Rectangles

R-Trees are similar except that they are used for multi-dimensional data and correlate objects together within the same node that are geometrically close. To do so a minimized bounding rectangle (of any dimension) is drawn around nodes in order to organize them. The leaves are pointers to the actual objects which may be stored on disk pages. The R-Tree allows for a spatial search for objects within a defined location. Tree levels are searched to find which MBR contains the data, recursively searching finer grained MBRs.
Starvation-Induced Changes in Motility, Chemotaxis, and Flagellation of Rhizobium meliloti

ABSTRACT The changes in motility, chemotactic responsiveness, and flagellation of Rhizobium meliloti RMB7201, L5-30, and JJ1c10 were analyzed after transfer of the bacteria to buffer with no available C, N, or phosphate. Cells of these three strains remained viable for weeks after transfer to starvation buffer (SB) but lost all motility within just 8 to 72 h after transfer to SB. The rates of motility loss differed by severalfold among the strains. Each strain showed a transient, two- to sixfold increase in chemotactic responsiveness toward glutamine within a few hours after transfer to SB, even though motility dropped substantially during the same period. Strains L5-30 and JJ1c10 also showed increased responsiveness to the nonmetabolizable chemoattractant cycloleucine. Cycloleucine partially restored the motility of starving cells when added after transfer and prevented the loss of motility when included in the SB used for initial suspension of the cells. Thus, interactions between chemoattractants and their receptors appear to affect the regulation of motility in response to starvation independently of nutrient or energy source availability. Electron microscopic observations revealed that R. meliloti cells lost flagella and flagellar integrity during starvation, but not as fast, nor to such a great extent, as the cells lost motility. Even after prolonged starvation, when none of the cells were actively motile, about one-third to one-half of the initially flagellated cells retained some flagella. Inactivation of flagellar motors therefore appears to be a rapid and important response ofR. meliloti to starvation conditions. Flagellar-motor inactivation was at least partially reversible by addition of either cycloleucine or glucose. During starvation, some cells appeared to retain normal flagellation, normal motor activity, or both for relatively long periods while other cells rapidly lost flagella, motor activity, or both, indicating that starvation-induced regulation of motility may proceed differently in various cell subpopulations.
Application note 28-9383-81 AA Label-free interaction analysis Studies of anti-angiogenic protein variants and screening of candidate tumor angiogenesis markers using Biacore

VD4 ++++ +++++ ++++ +++ In order to grow, solid tumors depend on the generation of new blood vessels to supply rapidly proliferating tumor cells with nutrients in a process termed angiogenesis. Angiogenesis requires an intricate network of signals between tumor cells, vascular endothelial cells (ECs) and the surrounding interstitium. Vascular endothelial growth factor (VEGF) and its receptors are important players in this network. The following examples from the literature show how label-free interaction analysis using BiacoreTM systems has helped investigators develop therapeutic strategies by providing binding data on anti-angiogenic antibodies or soluble variants of membrane-bound receptors.