Miracle-dz/UniFAR-FTUs huggingface dataset:
Protecting memory from misinformation: Warnings modulate cortical reinstatement during memory retrieval

Significance Exposure to misleading information can distort memory for past events (misinformation effect). Here, we show that providing individuals with a simple warning about the threat of misinformation significantly reduces the misinformation effect, regardless of whether warnings are provided proactively (before exposure to misinformation) or retroactively (after exposure to misinformation). In the brain, this protective effect of warning is associated with increased reactivation of sensory regions associated with the original event and decreased reactivation of sensory regions associated with the misleading information. These findings reveal that warnings can protect memory from misinformation by modulating reconstructive processes at the time of memory retrieval and have important practical implications for improving the accuracy of eyewitness testimony as well as everyday memory reports. Exposure to even subtle forms of misleading information can significantly alter memory for past events. Memory distortion due to misinformation has been linked to faulty reconstructive processes during memory retrieval and the reactivation of brain regions involved in the initial encoding of misleading details (cortical reinstatement). The current study investigated whether warning participants about the threat of misinformation can modulate cortical reinstatement during memory retrieval and reduce misinformation errors. Participants watched a silent video depicting a crime (original event) and were given an initial test of memory for the crime details. Then, participants listened to an auditory narrative describing the crime in which some original details were altered (misinformation). Importantly, participants who received a warning about the reliability of the auditory narrative either before or after exposure to misinformation demonstrated less susceptibility to misinformation on a final test of memory compared to unwarned participants. Warned and unwarned participants also demonstrated striking differences in neural activity during the final memory test. Compared to participants who did not receive a warning, participants who received a warning (regardless of its timing) demonstrated increased activity in visual regions associated with the original source of information as well as decreased activity in auditory regions associated with the misleading source of information. Stronger visual reactivation was associated with reduced susceptibility to misinformation, whereas stronger auditory reactivation was associated with increased susceptibility to misinformation. Together, these results suggest that a simple warning can modulate reconstructive processes during memory retrieval and reduce memory errors due to misinformation.
Keep Calm and Survive: Adaptation Strategies to Energy Crisis in Fruit Trees under Root Hypoxia

Plants are permanently facing challenges imposed by the environment which, in the context of the current scenario of global climate change, implies a constant process of adaptation to survive and even, in the case of crops, at least maintain yield. O2 deficiency at the rhizosphere level, i.e., root hypoxia, is one of the factors with the greatest impact at whole-plant level. At cellular level, this O2 deficiency provokes a disturbance in the energy metabolism which has notable consequences on the yield of plant crops. In this sense, although several physiological studies describe processes involved in plant adaptation to root hypoxia in woody fruit trees, with emphasis on the negative impacts on photosynthetic rate, there are very few studies that include -omics strategies for specifically understanding these processes in the roots of such species. Through a de novo assembly approach, a comparative transcriptome study of waterlogged Prunus spp. genotypes contrasting in their tolerance to root hypoxia was revisited in order to gain a deeper insight into the reconfiguration of pivotal pathways involved in energy metabolism. This re-analysis describes the classically altered pathways seen in the roots of woody fruit trees under hypoxia, but also routes that link them to pathways involved with nitrogen assimilation and the maintenance of cytoplasmic pH and glycolytic flow. In addition, the effects of root hypoxia on the transcription of genes related to the mitochondrial oxidative phosphorylation system, responsible for providing adenosine triphosphate (ATP) to the cell, are discussed in terms of their roles in the energy balance, reactive oxygen species (ROS) metabolism and aerenchyma formation. This review compiles key findings that help to explain the trait of tolerance to root hypoxia in woody fruit species, giving special attention to their strategies for managing the energy crisis. Finally, research challenges addressing less-explored topics in recovery and stress memory in woody fruit trees are pointed out.
Chitosan Films Incorporated with Exopolysaccharides from Deep Seawater Alteromonas sp.

Two Alteromonas sp. strains isolated from deep seawater were grown to promote the production of exopolysaccharides (EPS, E611 and E805), which were incorporated into chitosan solutions to develop films. The combination of the major marine polysaccharides (chitosan and the isolated bacterial EPS) resulted in the formation of homogenous, transparent, colorless films, suggesting good compatibility between the two components of the film-forming formulation. With regards to optical properties, the films showed low values of gloss, in the range of 5–10 GU, indicating the formation of non-glossy and rough surfaces. In addition to the film surface, both showed hydrophobic character, with water contact angles higher than 100 º, regardless of EPS addition. Among the two EPS under analysis, chitosan films with E805 showed better mechanical performance, leading to resistant, flexible, easy to handle films.
Recent Advances in the Development of Virus-Like Particle-Based Flavivirus Vaccines

Flaviviruses include several medically important viruses, such as Zika virus (ZIKV), Dengue virus (DENV), West Nile virus (WNV) and Japanese encephalitis virus (JEV). They have expanded in geographic distribution and refocused international attention in recent years. Vaccination is one of the most effective public health strategies for combating flavivirus infections. In this review, we summarized virus-like particle (VLP)-based vaccines against the above four mentioned flaviviruses. Potential strategies to improve the efficacy of VLP-based flavivirus vaccines were also illustrated. The applications of flavivirus VLPs as tools for viral detection and antiviral drug screening were finally proposed.
Heterogeneous expression of dopaminergic markers and Vglut2 in mouse mesodiencephalic dopaminergic nuclei A8–A13

Co‐transmission of glutamate by brain dopaminergic (DA) neurons was recently proposed as a potential factor influencing cell survival in models of Parkinson's disease. Intriguingly, brain DA nuclei are differentially affected in Parkinson's disease. Whether this is associated with different patterns of co‐expression of the glutamatergic phenotype along the rostrocaudal brain axis is unknown in mammals. We hypothesized that, as in zebrafish, the glutamatergic phenotype is present preferentially in the caudal mesodiencephalic DA nuclei. Here, we used in mice a cell fate mapping strategy based on reporter protein expression (ZsGreen) consecutive to previous expression of the vesicular glutamate transporter 2 (Vglut2) gene, coupled with immunofluorescence experiments against tyrosine hydroxylase (TH) or dopamine transporter (DAT). We found three expression patterns in DA cells, organized along the rostrocaudal brain axis. The first pattern (TH‐positive, DAT‐positive, ZsGreen‐positive) was found in A8–A10. The second pattern (TH‐positive, DAT‐negative, ZsGreen‐positive) was found in A11. The third pattern (TH‐positive, DAT‐negative, ZsGreen‐negative) was found in A12–A13. These patterns should help to refine the establishment of the homology of DA nuclei between vertebrate species. Our results also uncover that Vglut2 is expressed at some point during cell lifetime in DA nuclei known to degenerate in Parkinson's disease and largely absent from those that are preserved, suggesting that co‐expression of the glutamatergic phenotype in DA cells influences their survival in Parkinson's disease.
[Electroacupuncture improves learning-memory ability possibly by suppressing apoptosis and down-regulating expression of apoptosis-related proteins in hippocampus and cerebral cortex in immature mice with Alzheimer's disease].

OBJECTIVE To investigate the effect of electroacupuncture (EA) at "Baihui"(GV20), "Fengfu"(GV16) and bilateral "Shenshu"(BL23) on learning-memory ability, apoptosis in the hippocampus and expression of Aβ, Caspase 3, Bax and Bcl-2 proteins in the hippocampus and cerebral cortex in immature mice with Alzheimer's disease (AD), so as to explore its mechanism underlying improvement of AD. METHODS Forty APP/PS1 transgenic male young mice were equally randomized into model and EA groups and 20 C57BL/6J male young mice were used as the normal control. EA (10 Hz, about 2 mA) was applied to GV20-BL23 and GV16-BL23 for 20 min, once daily, 6 days a week for 16 weeks. The Morris water maze swimming test was used to evaluate the animals' learning-memory ability. Congo red staining and immunohistochemical staining were used to detect senile plaques in the hippocampus (dentate gyrus) and cerebral cortex tissues. Terminal deoxynucleotidyl transferase-mediated dUTP Nick-end Labeling (TUNEL) was used to detect the cellular apoptosis of hippocampus. The expression levels of apoptosis related factors Caspase 3, Bax and Bcl-2 were detected by Western blot. RESULTS After modeling, the escape latency of place navigation test of Morris water maze swimming tasks was significantly increased (P<0.05), while the number of platform crossing and residence time in the platform quadrant of spatial exploration test were significantly decreased in the model group in contrast to the normal control group (P<0.05). The number of apoptotic cells in the hippocampus and expression levels of Aβ, Caspase 3 and Bax proteins in the hippocampus and cerebral cortex were significantly up-regulated in the model group relevant to the normal control group (P<0.05). Following EA intervention, the escape latency of place navigation test of Morris water maze swimming tasks was significantly decreased (P<0.05), while the number of platform crossing and residence time in the platform quadrant of spatial exploration test were significantly increased in the EA group in contrast to the model group (P<0.05). The hippocampal apoptotic cells, the expression of Aβ, Caspase 3 and Bax proteins in hippocampus and cerebral cortex were evidently down-regulated in the EA group in contrast to the model group (P<0.05). Whereas the ratio of Bcl-2/Bax was significantly decreased in the model group relevant to the normal control group (P<0.05) and considerably increased in the EA group in contrast to the model group (P<0.05). No significant changes were found in the expression levels of Bcl-2 after modeling and after EA intervention (P>0.05). CONCLUSION EA of GV20, GV16 and BL23 can effectively improve the learning-memory ability in AD mice, which may be related to its function in inhibiting neuronal apoptosis in the hippocampus and down-regulating the expression levels of Aβ, Caspase 3 and Bax proteins in both hippocampus and cerebral cortex.
Transmembrane collagens—Unexplored mediators of epidermal‐dermal communication and tissue homeostasis

Tissue homeostasis is maintained through constant, dynamic and heterogeneous communication between cells and their microenvironment. Proteins that are at the same time active at the intracellular, cell periphery and deeper extracellular levels possess the ability to, on the individual molecular level, influence the cells and their microenvironment in a bidirectional manner. The transmembrane collagens are a family of such proteins, which are of notable interest for tissue development and homeostasis. In skin, expression of all transmembrane collagens has been reported and deficiency of transmembrane collagen XVII manifests with distinct skin phenotypes. Nevertheless, transmembrane collagens in skin remain understudied despite the association of them with epidermal wound healing and dermal fibrotic processes. Here, we present an overview of transmembrane collagens and put a spotlight on them as regulators of epidermal‐dermal communication and as potential players in fibrinogenesis.
Haemogram-Derived Indices for Screening and Prognostication in Critically Ill Septic Shock Patients: A Case-Control Study

This study aimed (1) to assess the diagnostic accuracy of neutrophil-to-lymphocyte (NLR), platelet-to-lymphocyte (PLR), monocyte-to-lymphocyte (MLR) and platelet count-to-mean platelet volume (PLT/MPV) ratios in predicting septic shock in patients on admission to the intensive care unit (ICU) and (2) to compare it with the role of C-reactive protein (CRP), procalcitonin (PCT) and lactate level. We also sought (3) to verify whether the indices could be useful in ICU mortality prediction and (4) to compare them with Acute Physiology and Chronic Health Evaluation II (APACHE II), Simplified Acute Physiology Score II (SAPS II) and Sequential Organ Failure Assessment (SOFA) scores. This retrospective study covered 138 patients, including 61 subjects with multi-organ failure due to septic shock (study group) and 77 sex- and age-matched controls. Septic patients had significantly higher NLR (p < 0.01) and NLR predicted septic shock occurrence (area under the ROC curve, AUROC = 0.66; 95% CI 0.58–0.74). PLR, MLR and PLT/MPV were impractical in sepsis prediction. Combination of CRP with NLR improved septic shock prediction (AUROC = 0.88; 95% CI 0.81–0.93). All indices failed to predict ICU mortality. APACHE II and SAPS II predicted mortality with AUROC = 0.68; 95% CI 0.54–0.78 and AUROC = 0.7; 95% CI 0.57–0.81, respectively. High NLR may be useful to identify patients with multi-organ failure due to septic shock but should be interpreted along with CRP or PCT. The investigated indices are not related with mortality in this specific clinical setting.
Fine-scale heterogeneity in Schistosoma mansoni force of infection measured through antibody response

Significance Schistosomiasis is one of the most common parasitic diseases in the world, and most infected people (90%) live in Africa. Global control efforts use measures of population-level transmission to target programs and assess progress toward elimination. Monitoring Schistosoma mansoni transmission has traditionally relied on examining stool with microscopy, which is difficult to scale in large programs and has low sensitivity as infection burdens decline. Our results show that antibody-based measures of transmission align well with stool-based measures, provide higher sensitivity at lower levels of transmission, and enable fine-scale estimates of force of infection by geography and age. The findings represent a major step toward use of serosurveillance to guide schistosomiasis control efforts in Africa. Schistosomiasis is among the most common parasitic diseases in the world, with over 142 million people infected in low- and middle-income countries. Measuring population-level transmission is centrally important in guiding schistosomiasis control programs. Traditionally, human Schistosoma mansoni infections have been detected using stool microscopy, which is logistically difficult at program scale and has low sensitivity when people have low infection burdens. We compared serological measures of transmission based on antibody response to S. mansoni soluble egg antigen (SEA) with stool-based measures of infection among 3,663 preschool-age children in an area endemic for S. mansoni in western Kenya. We estimated force of infection among children using the seroconversion rate and examined how it varied geographically and by age. At the community level, serological measures of transmission aligned with stool-based measures of infection (ρ = 0.94), and serological measures provided more resolution for between-community differences at lower levels of infection. Force of infection showed a clear gradient of transmission with distance from Lake Victoria, with 94% of infections and 93% of seropositive children in communities <1.5 km from the lake. Force of infection increased through age 3 y, by which time 65% (95% CI: 53%, 75%) of children were SEA positive in high-transmission communities—2 y before they would be reached by school-based deworming programs. Our results show that serologic surveillance platforms represent an important opportunity to guide and monitor schistosomiasis control programs, and that in high-transmission settings preschool-age children represent a key population missed by school-based deworming programs.
Landfast sea ice material properties derived from ice bridge simulations using the Maxwell elasto-brittle rheology

Abstract. The Maxwell elasto-brittle (MEB) rheology is implemented in the Eulerian finite-difference (FD) modeling framework commonly used in classical viscous-plastic (VP) models. The role of the damage parameterization, the cornerstone of the MEB rheology, in the formation and collapse of ice arches and ice bridges in a narrow channel is investigated. Ice bridge simulations are compared with observations to derive constraints on the mechanical properties of landfast sea ice. Results show that the overall dynamical behavior documented in previous MEB models is reproduced in the FD implementation, such as the localization of the damage in space and time and the propagation of ice fractures in space at very short timescales. In the simulations, an ice arch is easily formed downstream of the channel, sustaining an ice bridge upstream. The ice bridge collapses under a critical surface forcing that depends on the material cohesion. Typical ice arch conditions observed in the Arctic are best simulated using a material cohesion in the range of 5–10 kN m−2. Upstream of the channel, fracture lines along which convergence (ridging) takes place are oriented at an angle that depends on the angle of internal friction. Their orientation, however, deviates from the Mohr–Coulomb theory. The damage parameterization is found to cause instabilities at large compressive stresses, which prevents the production of longer-term simulations required for the formation of stable ice arches upstream of the channel between these lines of fracture. Based on these results, we propose that the stress correction scheme used in the damage parameterization be modified to remove numerical instabilities.
Shifts in aquatic insect composition in a tropical forest stream after three decades of climatic warming

The effects of climatic warming on tropical streams have received little attention, and field studies of such changes are generally lacking. Drifting insects from a Hong Kong forest stream were sampled for 36 months between 2013 and 2016, and compared with samples collected using identical methods in 1983–84. Mean air temperatures rose by ~0.5°C (0.17°C per decade) over this period. The stream drained an uninhabited protected area, so no climate‐change effects were confounded by anthropogenic disturbance. In total, 105 taxa and >77,000 individuals were collected. Richness of samples in the historic and contemporary datasets did not differ, but true diversity of drifting insects was highest in 1983–84, and declined between 2013–14 and 2015–16. There was considerable disparity in assemblage composition between 1983–84 and 2013–16, and smaller between‐year changes in the contemporary dataset. Nine indicator species of the historic dataset were identified. Most were mayflies, particularly Baetidae, which were greatly reduced in relative abundance in 2013–16. Diptera became more numerous, and tanypodine chironomids were the sole contemporary indicator taxon. Relative abundance of eight of 19 drifting species (comprising 60% of total insects) was lower in 2013–16, when the dominant baetid mayfly during 1983–84 had declined by almost 90%; only one of the 19 species occurred at higher abundance. Eight species were affected by seasonal temperature variability, but these responses were not correlated with any tendency to exhibit long‐term changes in abundance. Substantial shifts in composition, including declines in mayfly relative abundance and assemblage diversity, occurred after three decades of warming, despite the broad annual range of stream temperatures (~16°C) in Hong Kong. This contradicts the well‐known prediction that organisms from variable climates have evolved wider thermal tolerances that reflect prevailing environmental conditions. The observed compositional reorganization indicates that variability, rather than stability, may be typical of undisturbed tropical stream communities.
Youth‐friendly services and a mobile phone application to promote adherence to pre‐exposure prophylaxis among adolescent men who have sex with men and transgender women at‐risk for HIV in Thailand: a randomized control trial

Strategies are needed to curb the increasing HIV incidence in young men who have sex with men (YMSM) and transgender women (YTGW) worldwide. We assessed the impact of youth‐friendly services (YFS) and a mobile phone application (app) on adherence to pre‐exposure prophylaxis (PrEP) in YMSM and YTGW in Thailand.
Topic Modeling for Analyzing Patients’ Perceptions and Concerns of Hearing Loss on Social Q&A Sites: Incorporating Patients’ Perspective

Hearing loss is the most common human sensory deficit, affecting normal communication. Recently, patients with hearing loss or at risk of hearing loss are increasingly turning to the online health community for health information and support. Information on health-related topics exchanged on the Internet is a useful resource to examine patients’ informational needs. The ability to understand the patients’ perspectives on hearing loss is critical for health professionals to develop a patient-centered intervention. In this paper, we apply Latent Dirichlet Allocation (LDA) on electronic patient-authored questions on social question-and-answer (Q&A) sites to identify patients’ perceptions, concerns, and needs on hearing loss. Our results reveal 21 topics, which are both representative and meaningful, and mostly correspond to sub-fields established in hearing science research. The latent topics are classified into five themes, which include “sudden hearing loss”, “tinnitus”, “noise-induced hearing loss”, “hearing aids”, “dizziness”, “curiosity about hearing loss”, “otitis media” and “complications of disease”. Our topic analysis of patients’ questions on the topic of hearing loss allows achieving a thorough understanding of patients’ perspectives, thereby leading to better development of the patient-centered intervention.