Miracle-dz/UniFAR-FTUs huggingface dataset:
Reduced Macrophage Recruitment, Proliferation, and Activation in Colony-Stimulating Factor-1-Deficient Mice Results in Decreased Tubular Apoptosis During Renal Inflammation 1

Kidney tubular epithelial cell (TEC) death may be dependent on the number and activation state of macrophages (Mφ) during inflammation. Our prior studies indicate that activated Mφ release soluble mediators that incite TEC death, and reducing intrarenal Mφ during kidney disease diminishes TEC apoptosis. CSF-1 is required for Mφ proliferation and survival. We hypothesized that in the absence of CSF-1, Mφ-mediated TEC apoptosis would be prevented during renal inflammation. To test this hypothesis, we evaluated renal inflammation during unilateral ureter obstruction in CSF-1-deficient (Csf1op/Csf1op) mice. We detected fewer Mφ and T cells and less apoptotic TEC in the obstructed kidneys of Csf1op/Csf1op mice compared with wild-type (WT) mice. The decrease in intrarenal Mφ resulted from diminished recruitment and proliferation, not enhanced apoptosis. CSF-1 enhanced Mφ activation. There were far fewer activated (CD69, CD23, Ia, surface expression) Mφ in obstructed CSF-1-deficient compared with WT obstructed kidneys. Similarly, bone marrow Mφ preincubated with anti-CSF-1 receptor Ab or anti-CSF-1 neutralizing Ab were resistant to LPS- and IFN-γ-induced activation. We detected fewer apoptotic-inducing molecules (reactive oxygen species, TNF-α, inducible NO synthase) in 1) Mφ propagated from obstructed Csf1op/Csf1op compared with WT kidneys, and 2) WT bone marrow Mφ blocked with anti-CSF-1 receptor or anti-CSF-1 Ab compared with the isotype control. Furthermore, blocking CSF-1 or the CSF-1 receptor induced less TEC apoptosis than the isotype control. We suggest that during renal inflammation, CSF-1 mediates Mφ recruitment, proliferation, activation, and, in turn, TEC apoptosis.
A comparison of outcome from febrile neutropenic episodes in children compared with adults: results from four EORTC studies

The object of this study was to determine whether there were any differences between the ‘typical’ child with fever and neutropenia and their adult counterpart with regard to infection type and outcome, by analysis of 3080 patients, including 759 children < 18 years of age and 2321 adults. These represented patients randomized in previous trials, between 1986 and 1994, which compared empirical antibiotic regimens for fever in neutropenic patients. There were fewer childhood acute myeloid leukaemia patients than adults but more acute lymphoblastic leukaemia cases and more with solid tumours undergoing intensive myelosuppressive therapy. The children were less likely to be undergoing first induction therapy but the relative incidence of patients receiving relapse schedules or maintenance therapies were not significantly different in the two age groups. Children less frequently had a defined site of infection than adults and where they had a defined site there were more upper respiratory tract but fewer lung infections. There was a similar low incidence of shock at presentation in the two groups but the children's median neutrophil count was lower, and their median duration of granulocytopenia before the trial was shorter. The incidence of bacteraemia was similar, but clinically documented infection was less frequent and fever of unknown origin consequently more common in children. Children developed more streptococcal bacteraemias and fewer staphylococcal bacteraemias than adults (P = 0.003) but the relative incidence of various gram‐negative species was similar (P = 0.57). In general, the children had a better overall success rate and lower mortality than adults. Death from infection was only 1% in children versus 4% in adults (P = 0.001), and time to defervescence was shorter in children. In the younger age group, univariate logistic regression models showed high temperature, prolonged neutropenia before the trial and shock as prognostic indicators for the presence of bacteraemia. Solid tumour patients were significantly less likely to have a bacteraemia. Multivariate analysis confirmed the independent prognostic value of these indicators. Using the logistic equation of the selected model, the overall discriminant ability was poor. However, it was possible to identify a small subgroup without shock or high fever and with a short prior duration of neutropenia which carries a particularly low risk of bacteraemia, who could be considered for early discharge, monotherapy and shortened courses of antibodies, in prospective trials.
Oxytocin-stimulated phosphoinositide hydrolysis in human myometrial cells: involvement of pertussis toxin-sensitive and -insensitive G-proteins.

Phosphoinositide hydrolysis is important in mediating the actions of oxytocin and prostaglandin (PG) F2 alpha on uterine contractions during labour. We have measured the effect of oxytocin, PGF2 alpha and other agents on the formation of inositol phosphates (IPs) in cultured human myometrial cells labelled with [3H]inositol and on changes in intracellular free Ca2+ concentration ([Ca2+]i) in cells loaded with Fura-2. Oxytocin induced the formation of [3H]IPs in a concentration-dependent manner with an EC50 (concentration of agonist producing 50% of the maximal response) of 1.4 +/- 0.5 nmol/l (mean +/- S.E.M.). The maximal response was obtained with 1 mumol oxytocin/l and represented a stimulation of 670% over basal. PGF2 alpha also stimulated the formation of [3H]IPs and the response at 1 mumol/l was a 204% stimulation over basal. The effects of PGF2 alpha were independent of extracellular Ca2+ but the effect of oxytocin was reduced with low extracellular Ca2+. Cyclic AMP formation, induced by forskolin or PGE2, had no effect on the stimulated levels of [3H]IPs. Pertussis toxin (PT) reduced the oxytocin-stimulated formation of [3H]IPs in a concentration-dependent manner. The maximal effect of PT resulted in an 80% reduction in the formation of [3H]IPs. However, PGF2 alpha stimulation was not affected by PT treatment. To analyse the action of PT further, we studied its effect on oxytocin-induced changes in [Ca2+]i. The basal [Ca2+]i was 112 +/- 4 nmol/l (n = 225 cells) and was not affected by PT treatment (109 +/- 3 nmol/l; n = 200 cells). In the absence of PT, 1 mumol oxytocin/l increased [Ca2+]i to a peak of 522 +/- 26 nmol/l, and in PT-treated cells, the [Ca2+]i peak was reduced to 348 +/- 16 nmol/l. Similar inhibitory effects of PT were obtained at oxytocin concentrations ranging from 1 to 100 nmol/l. Our data suggest that in human myometrial cells, the oxytocin-induced production of [3H]IPs and increase in [Ca2+]i are mediated by a PT-sensitive G-protein. However, a significant fraction of the oxytocin response appears to be mediated by a PT-insensitive G-protein, possibly a member of the Gq family.
Anti-disturbance control for flexible spacecraft with time-varying input delay

This paper focuses on the anti-disturbance attitude control problem of flexible spacecraft with time-varying input delay. A composite hierarchical control structure is designed by combining delays-dependent H∞ control with disturbance-observer-based control (DOBC). The proposed composite method not only can compensate the disturbances caused by flexible appendages but also can reduce the effects of delay on attitude control. Specially, a decomposition coefficient of delay integral inequality is introduced in the process of solving functional, which may further reduce the design algorithm conservatism. Finally, numerical simulations are used to verify the effectiveness of the proposed method and to show the effects of time delay bound and decomposition coefficient on the closed-loop system performance.
The Trail Making Test, Part B: Cognitive Flexibility or Ability to Maintain Set?

The Trail Making Test (TMT) is a well-established test sensitive to impairment in multiple cognitive domains. There has been ambiguity about which cognitive demands are placed on the patient by TMT Part B over and above those required to perform TMT Part A. In particular, cognitive flexibility and ability to maintain a complex response set have been 2 competing hypotheses. This study preliminarily examined which of these 2 abilities primarily contributes to Part B performance. A total of 121 clinically referred Veterans Affairs patients were administered the TMT, as well as other tests of executive and other cognitive functions. Regression analyses were used to examine which tests predicted Part B performance above and beyond Part A performance. The results provide preliminary support for TMT Part B performance being more sensitive to cognitive flexibility (operationalized as Wisconsin Card Sorting Test [WCST], percent perseverative errors) than ability to maintain set (operationalized as WCST, failure to maintain set).
Clonogenic analysis reveals reserve stem cells in postnatal mammals: I. Pluripotent mesenchymal stem cells

Clonal populations of lineage‐uncommitted pluripotent mesenchymal stem cells have been identified in prenatal avians and rodents. These cells reside in the connective tissue matrices of many organs and tissues. They demonstrate extended capabilities for self‐renewal and the ability to differentiate into multiple separate tissues within the mesodermal germ line. This study was designed to determine whether such cells are present in the connective tissues of postnatal mammals. This report describes a cell clone derived by isolation from postnatal rat connective tissues, cryopreservation, extended propagation, and serial dilution clonogenic analysis. In the undifferentiated state, this clone demonstrates a high nuclear‐to‐cytoplasmic ratio and extended capacity for self‐renewal. Subsequent morphological, histochemical, and immunochemical analysis after the induction of differentiation revealed phenotypic markers characteristic of multiple cell types of mesodermal origin, such as skeletal muscle, smooth muscle, fat cells, cartilage, and bone. These results indicate that this clone consists of pluripotent mesenchymal stem cells. This report demonstrates that clonal populations of reserve stem cells are present in mammals after birth. Potential roles for such cells in the maintenance, repair, and regeneration of mesodermal tissues are discussed. Anat Rec 263:350–360, 2001. © 2001 Wiley‐Liss, Inc.
Indo-European Warfare

Abstract The Indo-European languages comprise the largest language family in the world and by the Late Bronze Age and Iron Age occupied a broad expanse of Eurasia from Ireland to western China and India. The inherited vocabulary of the Indo-European languages provides us with an image of the prehistoric language(s) that was spoken at least from the late Neolithic onwards and sheds light on the actual names of weapons, types of defensive architecture, terms for aggressive behaviour, trauma, institutions and poetic diction associated with warfare. In addition, there is also a body of ethnographic and mythological data that purports to provide a picture of the social organization and attitudes toward warriors shared by the earliest Indo-Europeans.
Crystal structure of Ultralente—A microcrystalline insulin suspension

Ultralente insulin has been one of the commercially most important insulin preparations in diabetes treatment over the last 50 years. It is a suspension of insulin microcrystals which dissolve slowly following subcutaneous injection. Because of the small crystal size of about 25 × 25 × 5 μm3 the atomic structure has been elusive until now. Here we present the crystal structures from Ultralente and their precursor microcrystals from the industrial manufacturing process. During this process insulin undergoes a conformational change within the microcrystals. Both structures show canonical folding of the insulin molecules but exhibit a number of new features when compared with other insulin structures. Surprisingly, we found that the Ultralente crystals bind the conservation agent methylparaben, which slows down dissolution of the crystals and thus contributes to the long duration of action. Proteins 2009. © 2008 Wiley‐Liss, Inc.
Zenith: Utility-Aware Resource Allocation for Edge Computing

In the Internet of Things(IoT) era, the demands for low-latency computing for time-sensitive applications (e.g., location-based augmented reality games, real-time smart grid management, real-time navigation using wearables) has been growing rapidly. Edge Computing provides an additional layer of infrastructure to fill latency gaps between the IoT devices and the back-end computing infrastructure. In the edge computing model, small-scale micro-datacenters that represent ad-hoc and distributed collection of computing infrastructure pose new challenges in terms of management and effective resource sharing to achieve a globally efficient resource allocation. In this paper, we propose Zenith, a novel model for allocating computing resources in an edge computing platform that allows service providers to establish resource sharing contracts with edge infrastructure providers apriori. Based on the established contracts, service providers employ a latency-aware scheduling and resource provisioning algorithm that enables tasks to complete and meet their latency requirements. The proposed techniques are evaluated through extensive experiments that demonstrate the effectiveness, scalability and performance efficiency of the proposed model.
Interaction of Rac Exchange Factors Tiam1 and Ras-GRF1 with a Scaffold for the p38 Mitogen-Activated Protein Kinase Cascade

ABSTRACT Tiam1 and Ras-GRF1 are guanine nucleotide exchange factors (GEFs) that activate the Rac GTPase. The two GEFs have similar N-terminal regions containing pleckstrin homology domains followed by coiled-coils and additional sequences that function together to allow regulated GEF activity. Here we show that this N-terminal region of both proteins binds to the scaffold protein IB2/JIP2. IB2/JIP2 is a scaffold for the p38 mitogen-activated protein (MAP) kinase cascade because it binds to the Rac target MLK3, the MAP kinase kinase MKK3, and the p38 MAP kinase. Expression of IB2/JIP2 in cells potentiates the ability of Tiam1 or Ras-GRF1 to activate the p38 MAP kinase cascade but not the Jnk MAP kinase cascade. In addition, Tiam1 or Ras-GRF1 binding to IB2/JIP2 increases the association of the components of the p38 MAP kinase signaling cassette with IB2/JIP2 in cells and activates scaffold-associated p38. These findings imply that Tiam1 and Ras-GRF1 can contribute to Rac signaling specificity by their ability to form a complex with a scaffold that binds components of one of the many known Rac effector pathways.
Structure of Ego-Alter Relationships of Politicians in Twitter

We analyze the ego-alter Twitter networks of 300 Italian MPs and 18 European leaders, and of about 14,000 generic users. We find structural properties typical of social environments, meaning that Twitter activity is controlled by constraints that are similar to those shaping conventional social relationships. However, the evolution of ego-alter ties is very dynamic, which suggests that they are not entirely used for social interaction, but for public signaling and self-promotion. From this standpoint, the behavior of EU leaders is much more evident, while Italian MPs are in between them and generic users. We find that politicians – more than generic users – create relationships as a side effect of tweeting on discussion topics, rather than by contacting specific alters.
CBCAnalyzer: inferring phylogenies based on compensatory base changes in RNA secondary structures

The CBCAnalyzer (CBC=compensatory base change) is a custom written software toolbox consisting of three parts, CTTransform, CBCDetect, and CBCTree. CTTransform reads several ct-file formats, and generates a so called "bracket-dot-bracket" format that typically is used as input for other tools such as RNAforester, RNAmovie or MARNA. The latter one creates a multiple alignment based on primary sequences and secondary structures that now can be used as input for CBCDetect. CBCDetect counts CBCs in all against all of the aligned sequences. This is important in detecting species that are discriminated by their sexual incompatibility. The count (distance) matrix obtained by CBCDetect is used as input for CBCTree that reconstructs a phylogram by using the algorithm of BIONJ. In this note we describe the features of the toolbox as well as application examples. The toolbox provides a graphical user interface. It is written in C++ and freely available at: http://cbcanalyzer.bioapps. biozentrum.uni-wuerzburg.de.
Thermodynamics as a theory of decision-making with information-processing costs

Perfectly rational decision-makers maximize expected utility, but crucially ignore the resource costs incurred when determining optimal actions. Here, we propose a thermodynamically inspired formalization of bounded rational decision-making where information processing is modelled as state changes in thermodynamic systems that can be quantified by differences in free energy. By optimizing a free energy, bounded rational decision-makers trade off expected utility gains and information-processing costs measured by the relative entropy. As a result, the bounded rational decision-making problem can be rephrased in terms of well-known variational principles from statistical physics. In the limit when computational costs are ignored, the maximum expected utility principle is recovered. We discuss links to existing decision-making frameworks and applications to human decision-making experiments that are at odds with expected utility theory. Since most of the mathematical machinery can be borrowed from statistical physics, the main contribution is to re-interpret the formalism of thermodynamic free-energy differences in terms of bounded rational decision-making and to discuss its relationship to human decision-making experiments.
Using the Value Discipline Strategy Typology to Describe Services Marketing Strategies

A qualitative study is undertaken where the Value Discipline Strategy typology (Treacy and Wiersema, 1995) is used to describe international student recruitment (ISR) marketing strategies used by educational institutions. Interview respondents could easily identify with the typology and this suggests applicability for use in the services industry. No clear differences in types of strategy used are found between different education sectors although there is some evidence to indicate that the length of institutional recruitment experience may be a factor in determining strategic orientation. Findings presented are from a sample of secondary schools and universities in Australia and New Zealand.
Beta Endorphin and Alcohol Urge Responses in Alcoholic Patients Following an Acute Bout of Exercise

Objective: To examine the effects of low intensity exercise on beta endorphin (β-E) levels and urge for alcohol in alcoholic patients. Methods: Nine alcoholic patients (M= 41.2 + 6.7 yrs) and 9 healthy controls (M=38.2 + 10.7 yrs) exercised for 30 minutes at a low intensity (61.1 + 4.9 % of their maximum heart rate). Blood was collected prior to and immediately following exercise and was analyzed for a complete blood count (CBC), β-E and lactic acid. Furthermore, an alcohol urge questionnaire was filled by the subjects prior to and immediately following exercise. Results: Data analysis revealedthat β-E levels were significantly lower in alcoholic patients whereas exercise resulted in significant (p< .001) increases in β-E (pre: 1.57 + 0.39 pmol/L, post: 4.8 + 1.6 pmol/L) only in alcoholic patients. Lactic acid increased significantly in both groups. There was a 17% decrease in alcohol urge in alcoholic patients without however this difference being significant. No differences in the parameters assessed through the CBC were seen between the two groups whereas exercise resulted in significant increases in red blood cells, hemoglobin and hematocrit in both groups. Conclusion: These results indicate that a bout of low intensity exercise affects the endogenous opioids in alcoholic patients.
Compression for venous leg ulcers.

OBJECTIVES To assess the effectiveness and cost-effectiveness of compression bandaging and stockings in the treatment of venous leg ulcers. SEARCH STRATEGY Searches of 19 databases, hand searching of journals, conference proceedings and bibliographies. Manufacturers of compression bandages and stockings and an Advisory Panel were contacted for unpublished studies. SELECTION CRITERIA Trials that evaluated compression bandaging or stockings, as a treatment for venous leg ulcers. There was no restriction on date or language. Ulcer healing was the primary endpoint. DATA COLLECTION AND ANALYSIS Details of eligible studies were extracted and summarised using a data extraction sheet. Data extraction was verified by two reviewers independently. MAIN RESULTS Twenty two trials reporting 24 comparisons were identified. Compression was more effective than no compression (4/6 trials). When multi-layered systems were compared, elastic compression was more effective than non-elastic compression (5 trials). There was no difference in healing rates between 4-layer bandaging and other high compression multi-layered systems (3 trials). There was no difference in healing rates between elastomeric multi-layered systems (4 trials). Multi-layered high compression was more effective than single layer compression (4 trials). Compression stockings were evaluated in two trials. One found a high compression stocking plus a thrombo stocking to be more effective than a short stretch bandage. The second small trial reported no difference between the compression stockings and Unna's boot. There were insufficient data to draw conclusions about the relative cost-effectiveness of different regimens. REVIEWER'S CONCLUSIONS Compression increases ulcer healing rates compared with no compression. Multi-layered systems are more effective than single-layered systems. High compression is more effective than low compression but there are no clear differences in the effectiveness of different types of high compression.
Mechanical Strain Induces Expression of C-Reactive Protein in Human Blood Vessels

C-reactive protein (CRP) is a powerful independent risk factor for cardiovascular diseases. Elevated mechanical strain on vessels induces the local expression of proinflammatory cytokines. We hypothesized that mechanical strain on vessels may induce local CRP expression. Human saphenous vein and internal mammary artery (IMA) rings were stretched in vitro with a mechanical strength of 1, 3, or 5 g. Reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay results showed that mechanical stretching significantly induced CRP mRNA and protein expression in the saphenous vein and IMA rings in a strength-dependent manner reaching a maximum at a mechanical strength of 3 g, but CRP expression returned at strengths of >5 g. In vessels, mechanical strain-induced CRP expression was blocked by two stretch-activated ion channel (SAC) blockers: GdCl3 and streptomycin. Mechanical strain also increased activation of nuclear factor κB (NF-κB), which was detected with a nonradioactive NF-κB p50/p65 EZ-TFA transcription factor assay. Mechanical strain-induced NF-κB activation was blocked by SAC blockers and the NF-κB inhibitor (SN50, H-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Val-Gln-Arg-Lys-Arg-Gln-Lys-Leu-Met-Pro-OH). SN50 also blocked mechanical strain-induced CRP expression in vessels. In conclusion, mechanical strain induces CRP expression in IMAs and saphenous veins by activating the SAC-induced NF-κB pathway.