Miracle-dz/UniFAR-FTUs huggingface dataset:
INHIBITORY EFFECTS OF ESSENTIAL AMINO ACIDS ON THE PEAK CHEMILUMINESCENCE OF THE CELL-FREE SYSTEMS

The inhibitory effect of essential amino acids in aqueous solutions, on the peak chemiluminescence of hydrogen peroxide (H2O2), hypochlorous (HOCl) (derived from NaOCl) or peroxynitrite (ONOO) was evaluated by using flow injection analysis (FIA)-luminol chemiluminescence (CL) method. The chemiluminescence was measured as the luminometer output in mV and the effects of amino acids were considered by the depression of the signal from its uninhibited level and expressed as the percentage attenuation of the peak chemiluminescence due to the oxidant. The luminol-H2O2, HOCl or ONOOinduced CL signal was inhibited by either amino acids or ascorbate (well characterized, chain-breaking antioxidant) in a concentration-dependent manner. The log IC50 values of the inhibitor effect of amino acids were calculated and found that histidine and tryptophan displayed the most potent inhibitory activity against H2O2 or HOCl induced peak chemiluminescence, respectively. Results were concluded that the inhibitory effect of the essential aminoacids on the peak chemiluminescence might represent their free radical scavenger activity.
RAMA1 is a novel kinetochore protein involved in kinetochore-microtubule attachment

During mitosis, kinetochores need to attach to microtubules emanating from spindle poles. Several protein complexes have been shown to mediate the kinetochore-microtubule interaction. However, with the continually growing number of newly identified kinetochore proteins, it is unclear whether all major components of the kinetochore-microtubule interface have been identified. We therefore performed a high-throughput RNAi screen to identify additional factors involved in kinetochore-microtubule attachment, and identified RAMA1 as a novel regulator of this process. Depletion of RAMA1 results in severe chromosome alignment defects and a checkpoint-dependent mitotic arrest. We show that this is due to reduced kinetochore-microtubule attachments. RAMA1 localizes to the spindle and to outer kinetochores throughout all phases of mitosis and is recruited to kinetochores by the core kinetochore-microtubule attachment factor Hec1. Interestingly, unlike Hec1, the association of RAMA1 with kinetochores is highly dynamic, suggesting that it is not a structural component of the kinetochore. Consistent with this, all other kinetochore proteins tested do not require RAMA1 for their kinetochore localization. Taken together, these results identify RAMA1 as a novel kinetochore protein and suggest that RAMA1 may have a direct role in mediating kinetochore-microtubule interactions.
Caffeine enhances radiosensitization to orthotopic transplant LM3 hepatocellular carcinoma in vivo

The aim of this study was to determine whether caffeine enhanced radiosensitization in an orthotopic transplant of LM3 human hepatocellular cancer in nude mice. LM3 hepatocellular carcinoma cells were infected with red fluorescent protein and irradiated, and cell cycle distribution and survival fraction were detected. A nude mouse model of orthotopic transplant of red fluorescent protein‐expressing LM3 hepatocellular cancer was established. Nude mice were divided into four groups: control (NS); caffeine (Caff) alone; irradiation (IR) alone; and caffeine + IR (Caff + IR). Tumor growth curves were described. Expression of cyclin and apoptosis were evaluated by analysis of phosphorylated cyclin dependent kinase 1 (CDC2) Tyr15 (CDC2‐Tyr15‐P), cyclinB1, TUNEL staining, and caspase‐3. Caffeine abrogated IR‐induced G2 phase arrest and decreased survival of irradiated LM3 cells. Caffeine enhanced radiosensitivity of LM3 hepatocellular cancer in vivo. Tumor growth delay time in the Caff + IR group was 14.3 days compared with the NS group, 14.1 days compared with the Caff alone group, and 7.2 days compared with the IR alone group. At 15 Gy, expression of CDC2‐Tyr15‐P in the Caff + IR group (26.0 ± 8.9%) was significantly lower than in the IR alone group (68.4 ± 10.6%), expression of cyclinB1 and proportion of TUNEL‐positive cells in the Caff + IR group (30.4 ± 8.7% and 59.2 ± 9.5%, respectively) was significantly higher than in the IR alone group (7.0 ± 3.7% and 24.2 ± 7.2%, respectively), expression of caspase‐3 was consistent with the TUNEL staining results. This study suggested that caffeine might enhance the radiosensitivity of LM3 hepatocellular cancer in vivo, and may be feasible for further clinical applications. (Cancer Sci 2010)
Numerical simulation of flow past a square cylinder

A study of the behavior of a square cylinder subjected to an upstream uniform air flow is presented. The present investigation uses commercial CFD software FLUENT 6.2. 16 to simulate time-resolved flow field using constitutive relationships suitable for laminar flows. Variation of flow induced lift force coefficient is used to estimate shedding frequency and resultant mean structural vibration for the Reynolds number range of 2000-8000. Simulation results in terms of limit cycle oscillation amplitude are compared with pre-existing experimental data.
Sampled-data filter design for linear parameter varying systems

In the present paper, we address the sampleddata filtering synthesis problem for continuous-time linear parameter varying (LPV) systems. The filtering error system obtained from augmenting the original LPV system and the sampled-data filter is a hybrid system. The design objective is to guarantee the system stability and a prescribed level of the induced energy-to-energy gain (or H∞ norm) from the disturbance input to the estimation error. To this end, we employ the concept of lifting to derive an equivalent discrete-time LPV system. The discretizing method using the lifting approach is such that it would preserve the H∞ norm. The viability of the proposed design method to cope with variable sampling rates will be shown through numerical examples, where reliable estimation of the LPV system outputs can be achieved.
Initial and steady-state effects of diphenhydramine and loratadine on sedation, cognition, mood, and psychomotor performance.

BACKGROUND The classic, first-generation histamine1-receptor antagonists used to treat allergic disorders frequently cause sedation. In contrast, sedation is reduced or absent after administration of recommended doses of second-generation histamine1-receptor antagonists. We measured the initial and steady-state effects of diphenhydramine, a first-generation antihistamine, and loratadine, a second-generation antihistamine, by means of a comprehensive battery of psychometric tests that mirror real-world tasks. METHODS Healthy volunteers (N = 98) were randomly assigned in a double-blind fashion to receive loratadine (n = 33), diphenhydramine (n = 32), or placebo (n = 33). A computerized test battery was administered at baseline, on day 1 after administration of the initial dose, and on days 3 and 5. RESULTS After the initial dose, subjects taking diphenhydramine demonstrated poorer cognitive performance than subjects taking loratadine or placebo on tasks of divided attention, working memory, speed, and vigilance. Subjects taking diphenhydramine also reported greater fatigue and sleepiness and lower levels of motivation, and rated the quality of their performance as lower than subjects taking loratadine or placebo. On day 3, subjects taking diphenhydramine continued to show more fatigue and lower motivation, and rated the quality of their test performance as poorer than subjects taking loratadine or placebo. There were no differences between loratadine and placebo after the initial dose or steady-state (day 5) dosing for any measure of cognitive or psychomotor test performance, mood, or sedation. CONCLUSIONS Patients taking diphenhydramine may be at risk of lapses and significant errors that may lead to potential hazards and decreased work productivity.
Recent advances in pancreatic surgery

Purpose of review To appraise the recent literature dealing with important advances in the field of pancreatic surgery. Recent findings Surgical care for pancreatic cancer patients remains fractured, with imperfect patient selection and ongoing bias in referral patterns based on socioeconomic factors. Analysis of readmissions after pancreatectomy reveals it to be a poor quality of care metric. More extensive pancreatic resections lead to higher morbidity. Intraductal papillary mucinous neoplasm and pancreatic neuroendocrine tumor biology affect patient outcomes and suggest the need for better diagnostic approaches for these entities. Perioperative drainage still has a role during Whipple pancreaticoduodenectomy on the basis of the results of a randomized controlled trial. Laparoscopic and robotic approaches married with new emerging technologies have the potential to transform the practice of pancreatic surgery. Summary Pancreatic surgery is a rapidly evolving field with the promise to significantly improve outcomes for patients with a variety of pancreatic diseases in the future.
Prevalence, risk factors and treatment for substance abuse in older adults

Purpose of review This paper briefly outlines new research on the epidemiology of alcohol and drug use in the older population, describes mental and cognitive consequences of substance use and summarizes recent treatment trials for alcohol dependence. Recent findings Cross-sectional and longitudinal studies indicate that alcohol use is less prevalent in older groups, and it decreases over time. Comorbidity (alcohol and other drugs, alcohol with mental health disorders) is high. An inverse-U-shaped curve describes the association between alcohol consumption and cognitive impairment with increased impairment for abstainers and high users as compared with moderate users. Trials of alcohol use in the older population are rare, but they suggest that brief patient education may reduce drinking levels in primary care populations, and that, for some users, integrated primary care may be more useful than referral to specialist care. Summary The basis for higher risk in abstainers is not resolved. More randomized controlled trials are needed, which target alcohol use and dependence and also focus on comorbid disorders.
Microdosing Clinical Study: Pharmacokinetic, Pharmacogenomic (SLCO2B1), and Interaction (Grapefruit Juice) Profiles of Celiprolol Following the Oral Microdose and Therapeutic Dose

The authors evaluated the contribution of the SLCO2B1 polymorphism to the pharmacokinetics of celiprolol at a microdose (MD) and therapeutic dose (TD) and compared pharmacokinetic proportionality between the 2 dose forms in 30 SLCO2B1 genotype‐matched healthy volunteers. Three drugs (celiprolol, fexofenadine, and atenolol) were orally administered as a cassette dosing following the MD (totally 97.5 μg) and then a TD (100 mg) of celiprolol, with and without grapefruit juice. The mean AUC0–24 of celiprolol was lower in SLCO2B1*3/*3 individuals (775 ng·h/mL) than in *1/*3 (1097 ng·h/mL) and *1/*1 (1547 ng·h/mL) individuals following the TD, and this was confirmed in population pharmacokinetic analysis with statistical significances; however, SLCO2B1 genotype‐dependent differences disappeared following the MD. Dose‐normalized AUC of celiprolol at the MD was much lower than that at the TD, explained by the saturation of the efflux transporter. Thus, the effect of SLCO2B1 polymorphism on the AUC of celiprolol clearly observed only at the TD may be due to the saturation of the efflux transport systems.
ETIC : un SGBD pour la CAO dans un environnement partagé. (ETIC : a CAD DBMS in shared environment)

Le systeme de gestion de bases de donnees propose se base sur un modele de donnees oriente objet. Il permet la definition dynamique des objets et des types d'objet et l'expression de contraintes sur ces types. Il offre le concept de specialisation. Un ensemble de fonctionnalites pour definir, gerer et controler les activites d'une equipe de concepteurs collaborant a la realisation d'un projet est propose. De plus, un modele de versions permettant de manifester, caracteriser et retrouver les versions des objets CAO est defini
Using hand performance measures to predict handedness

Handedness is defined by the individual's preference to use one hand predominately for unimanual tasks and the ability to perform these tasks more efficiently with one hand (Corey, Hurley, & Foundas, 2001). It is important to use performance variables to measure handedness because they are more objective than traditional hand preference questionnaires (Bryden, Pryde, & Roy, 2000a). The current study develops a predictive model of handedness as measured by the Waterloo Handedness Questionnaire (WHQ) using several performance indicators of handedness. A total of 120 individuals (60 right-handers and 60 left-handers) were asked to complete four performance-based tasks: the Grooved Pegboard (GP), the Annett pegboard (AP), finger tapping (FT), and grip strength (GS) as well as an observational measure of preference, the Wathand Box Test (WBT). Backward linear regression analysis showed that the Wathand Box measure and the laterality quotients for several performance measures (GP place, AP, and FT) combined to act as the most accurate predictors of hand preference. The predictive model of handedness developed is as follows: WHQ = -2.760- – 0.667(GP place) + 0.809(FT) + 0.234(WBT) – 0.748(AP) with an explained variance of 0.836. These results illustrate, as Corey et al. (2001) suggested, that the best predictive model of handedness combines preference measures and several performance measures that tap into different elements of motor performance. By developing this model, it is possible to get an accurate measure of handedness using objective measures.
Simplified, high performance transceiver for phase modulated RFID applications

In this paper results are presented for a simple yet highly sensitive transceiver for phase modulated RFID applications. This is an advance on other simple RFID readers which can only operate with amplitude shift keyed (ASK) signals. Simple circuitry is achieved by the use of a novel injection locked PLL configuration which replaces the standard superhet type architecture normally used. The transceiver is shown to operate with a number of phase modulation modes which have certain advantages relating to distance to target. The paper concludes with practical results obtained for the transceiver when operated within a backscatter RFID application. A unique advantage of this transceiver is its complete immunity to the problem of TX/RX isolation, allowing for long ranges, estimated to be in the region of 80m at 1 GHz, to be achieved even in the presence of a simple backscatter target.
OPEN ACCESS ECHOCARDIOGRAPHY: A PROSPECTIVE AUDIT OF REFERRAL PATTERNS FROM PRIMARY CARE

Following recently published recommendations and guidelines, a prospective audit of 222 consecutive patients referred for open access echocardiography was conducted over a period of three months in a large district general hospital in the UK. Our study demonstrated the waiting time for an open access echocardiogram to be shorter than the waiting time for the outpatient clinic, which allowed identification of clinically significant cardiac disease sooner, leading to early advice on patient management. Specialist referral was avoided by the inclusion of management comments by a cardiologist in the technical echocardiogram report. We showed that open access echocardiography for detection of left ventricular systolic function, should be performed only if the ECG is abnormal, confirming previous reports. ECG interpretation in primary care is unreliable. In view of limited resources, hospitals should vigorously screen referrals for open access echocardiography.
Interpretation of the band-structure results for elastic and acoustic waves by analogy with the LCAO approach.

The band-structure results for elastic and acoustic waves propagating in a composite (consisting of periodically placed spheres in a host material) are analyzed by employing the frequency dependence of the scattering cross section from a single sphere. Two limiting modes of propagation can be visualized. According to the first the wave propagates mainly through the host material; according to the second the wave hops coherently from a sphere to its neighbors using the resonances in the single-sphere scattering cross section. This second mode is the analog of the linear combination of atomic orbitals in electronic propagation, with the atomic orbitals replaced by the resonances.
Making sense of it all: implementing an emerging KM technology within the organisation

Organisations have access to unreadable volumes of textual data and yet individuals are required to make sense of emerging practices and events from this blizzard of information. Tools that provide an overview of unstructured information to aid higher level understanding are now easily available. These category mapping tools have not been widely used. This paper describes the implementation and use of one such tool within a large commercial research organisation. We highlight two aspects of sense-making that come into play. First, the organisation struggles to make sense of what it does and what it does not know in the context of a surfeit of information. Second, in order to exploit appropriate tools in this process, the organisation must also make sense of the value and use of these tools.
Dynamic displacement current in subnanosecond breakdowns in an inhomogeneous electric field

The aim of the work was to take measurements during subnanosecond breakdowns with a time resolution no worse than 100 ps. The current through gaps filled with atmospheric pressure air was investigated during the generation of runaway electrons and X-rays. Three generators, when ensuring the formation of a runaway electrons preionized diffuse discharge (REP DD), were used. It is confirmed that during a subnanosecond voltage rise time, the amplitude of the dynamic displacement current can be higher than 4 kA. It is demonstrated that during the generation of supershort avalanches electron beams with amplitudes of 10 A and more. The conductivity in the air gap at the breakdown stage is ensured by the ionization wave, whose front propagates from the electrode of small curvature radius, and by the dynamic displacement current between the ionization wave front and the plane electrode.
How to represent a repetitive control system in the state space

Repetitive control is a specialized control scheme to track and/or attenuate a periodic reference trajectories and/or disturbances. Most research results about repetitive control have been performed in the frequency domain. In this paper, we present a method to represent repetitive control systems in the state space. The problem to describe a repetitive control system in the state space is equivalent to representing a q filter in the repetitive controller, which is a typical time-delayed system, as a state equation. The obtained results will be used to apply a lot of control theories in the state space to design of repetitive control systems.
GT-FLMin: Un Outil Graphique pour l'Extraction de Liens Fréquents dans les Réseaux Sociaux

Dans le domaine des reseaux sociaux, la recherche de motifs frequents correspond a l'extraction de sous-graphes retrouves frequemment dans un reseau ou un ensemble de reseaux. Contrairement aux approches classiques qui s'appuient uniquement sur des regularites structurelles, nous presentons dans ce travail l'outil GT-FLMin, capable d'exploiter les informations sur la structure pour extraire des regularites dans les liens qui connectent des noeuds similaires du point de vue de leurs caracteristiques. Nous appelons de tels motifs des liens frequents.
KATP Channel Mutations and Neonatal Diabetes

Since the discovery of the KATP channel in 1983, numerous studies have revealed its physiological functions. The KATP channel is expressed in various organs, including the pancreas, brain and skeletal muscles. It functions as a “metabolic sensor” that converts the metabolic status to electrical activity. In pancreatic beta-cells, the KATP channel regulates the secretion of insulin by sensing a change in the blood glucose level and thus maintains glucose homeostasis. In 2004, heterozygous gain-of-function mutations in the KCNJ11 gene, which encodes the Kir6.2 subunit of the KATP channel, were found to cause neonatal diabetes. In some mutations, diabetes is accompanied by severe neurological symptoms [developmental delay, epilepsy, neonatal diabetes (DEND) syndrome]. This review focuses on mutations of Kir6.2, the pore-forming subunit and sulfonylurea receptor (SUR) 1, the regulatory subunit of the KATP channel, which cause neonatal diabetes/DEND syndrome and also discusses the findings of the pathological mechanisms that are associated with neonatal diabetes, and its neurological features.
SCIAMACHY VALIDATION SUMMARY

In early 2004, preliminary validation was conducted for all SCIAMACHY data products: those generated operationally by ESA’s Envisat ground segment and those generated non-operationally at several scientific institutes. Results as presented and discussed during the second Workshop for Atmospheric Chemistry Validation of Envisat (ACVE-2) in May 2004, are summarised here. The main conclusion is that the SCIAMACHY products have improved considerably since the First Envisat Validation Workshop in December 2002, both in number and in quality. All validation results are however still preliminary in the sense that: a) data sets from longer periods with better sampling are necessary to detect seasonally varying errors and other medium-scale effects; b) not all available validation sources were properly used, due to lack of time; and c) comparison results reported here do not always reflect the expected final quality of the products as some of the SCIAMACHY data products are not generated with state-of-the-art processors (due e.g. to delays in the delivery of accurate level-1 data). Various recommendations for product improvement have been collected during the meeting and are summarised here. Note: Due to the short time available for validation before the ACVE-2, several contributions on SCIAMACHY validation in this proceedings have evolved since their presentation at the ACVE-2, so that conclusions for individual products may be different than in the original presentations. This summary paper is however mainly based on the presentations, and not on the papers in these proceedings. Therefore, we refer you to the separate product papers for more up-to-date conclusions on each product.