Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of angiotensin II on the expression of the early growth response gene c-fos and DNA synthesis in human vascular smooth muscle cells

Objectives The aims of this study were to characterize the angiotensin II receptor subtype present on vascular smooth muscle cells from human saphenous vein and to assess the effect of angiotensin II on the expression of the early growth response gene c-fos and on DNA synthesis. Methods and results Using radioligand binding studies, we have defined the angiotensin II receptors present on these cells as being predominantly of the AT1 subtype. Angiotensin II increased peak intracellular calcium levels by 126±16nmol/l (mean±SEM) in 17/49 cultures. Angiotensin II induced c-fos expression in a concentration- dependent manner only in cultures that exhibited an intracellular calcium transient in response to stimulation with angiotensin II. The induction of c-fos was inhibited by the selective AT1 antagonist losartan in accordance with the binding studies. Angiotensin II stimulated DNA synthesis with a maximal increase of 66.4% ± 20.5% over serum-free levels at 1 nmol/l (mean ± SEM, n = 6, P< 0.05). DNA synthesis declined with increasing angiotensin II concentration, falling to control values at 1 µmol/l, suggesting that a growth-inhibitory influence may counterbalance the stimulatory effect that is observed at lower concentrations. Conclusion Vascular smooth muscle cells from human saphenous vein possess predominantly AT1 receptors and in response to angiotensin II show an induction of c-fos and a modest increase in DNA synthesis.
A Chronicle Review of Nonsilicon (Sn, Sb, Ge)‐Based Lithium/Sodium‐Ion Battery Alloying Anodes

DOI: 10.1002/smtd.202000218 and zero emission. [3–5] Both traditional and emerging automobile enterprises are investing considerable resources to develop the new-energy vehicles. Moreover, the imminent exhaustion of fossil fuel resources and increasing environmental problems influence every corner of the Earth, which needs clean energy to improve such predicament. [6,7] At present, solar and wind energy have been developed to a mature stage and applied globally. However, how to integrate these renewable but inconstant energies into electrical supply grids smoothly and safety is still a big challenge. [8] Therefore, developing low-cost, large-scale electrical energy storage (EES) is considered as the necessary strategy and key enabler for future smart and green grids. [9,10] All these products need a high energy density power source for longer endurance time and better efficiency. The invention of the lithium-ion battery (LIB) in the 1990s started a new era, and a better portable power source has always been the persistent pursuit for both academic and industrial worlds in the following years. The birth of LIB endured several tens of years coupled with the efforts from top scientists all over the world.
Evaluation of molecular detection for viral hemorrhagic infection cases of yellow fever and dengue fever in Côte d'Ivoire 2010-2012, West Africa.

Background: Viral hemorrhagic fevers are emergent and endemic in Africa and in South America. In Cote d’Ivoire, Yellow fever cases were reported yearly and the distribution of mosquitoes in the country are the main factors for high incidence of Flaviviruses. The poorly reporting of viral hemorrhagic fever cases in some regions, the lack of international interest land and the underestimation of molecular surveillance method contribute to increase the risk for public health. Objectives: To evaluate the performance of molecular diagnostic methods in national surveillance Short Communication Solange et al.; BJMMR, 5(3): 396-403, 2015; Article no.BJMMR. 2015.043 397 of two emergent Flaviviruses, Yellow fever virus and Dengue virus in Cote d’Ivoire. Study Design: 63 sera from suspected cases in 2010-2011 of viral hemorrhagic fevers were analyzed to detect viral RNA of Flaviviruses and to compare the results in three different methods. Results and Conclusion: The Flavivirus RT-PCR has showed the high molecular detection by12% and 6% for real time PCR. The methods are specific and high sensitive for the screening of tick and mosquito-borne Flaviviruses in clinical samples. This study confirms the high circulation of Flaviviruses and the introduction of Dengue virus in Cote d’Ivoire. The combination of real time PCR and the Flavivirus RT-PCR contribute to ameliorate the detection panel of molecular detection in Cote d’Ivoire and was a suitable method for the National Laboratory Reference.
Wound Effects on Cytodifferentiation in Hardwood Xylem

The wound effects on cytodifferentiation in hardwood xylem were studied by means of periodical observation of wound tissue formation after a pin insertion into the stem of poplar. The mitotic reactivation of ray parenchyma cells was similar to that in conifers. These ray cell derivatives easily invaded other cells creating the impression of septate fibres. Conspicuous abnormalities were found in the differentiation of those fusiform cells which were situated in the zone of xylem mother cells at the time of wounding and those originating from cambial initials for several days after wounding. In the former zone, fusiform cells were prevented from differentiating into vessel elements after dividing transversely several times in the zone adjacent to the injury ; fusiform cells in the area extending several millimetres longitudinally were variously modified morphologically after the frequent transverse divisions in the xylem mother cell zone: they showed various transitional patterns from vessel element-like through tracheid-like, and axial parenchyma-cell-like to fibre-like. These observations suggest that the direction of cytodifferentiation is determined in the cambial initials or the neighbouring xylem mother cells, and is controlled by certain substances, which may change in concentration through the wounding stimulus, bringing about the modification in cytodifferentiation. Wound reaction of hardwood (i .e., woody dicotyledons) was thus completely different from the regeneration of vascular system in injured herbaceous dicotyledons.
Admission hyperglycemia and the no-reflow phenomenon in patients with acute myocardial infarction

Background: Coronary artery disease (CAD) is the single most common cause of death in the developed world, responsible for about one in every six deaths; 15.5 million person’s>20 years of age in the USA have coronary heart disease (CHD). No-reflow phenomenon defined classically as the lack of myocardial perfusion despite opening up the epicardial vessel in the setting of PPCI occurring in 10 % of cases of PPCI and associated with increase in 30 days mortality caused by multiple factors that lead to micro vascular obstruction and endothelial disruption by different mechanism like atherothrombotic embolization, ischemic injury, reperfusion injury and susceptibility of coronary microcirculation to injury. Objective: The aim of the study to is to investigate the correlation between admission blood glucose level and the no-reflow phenomenon in patients with AMI undergoing Primary PCI. Patients and methods: This study was Cross-sectional study which was conducted on patients presenting with acute ST segment elevation myocardial infarction who underwent primary PCI. The study included 280 patients presented with acute MI within less than 24 hours from onset of symptoms. All study population will undergo full medical history, full clinical examination, Blood sample and chemistry, electrocardiography, transthoracic echocardiography and coronary angiography. Results: Admission hyperglycemia with AMI among AMI patients was independent predictor for incidence of no-reflow. Diabetics after classified into hyperglycemic and non-hyperglycemic, hyperglycemic in diabetics has significant high no-reflow rate compared to non-diabetics. Conclusion: We established an association between hyperglycemia and the no-reflow phenomenon.
Esterification and Transesterification Catalysed by Cutinase in Reverse Micelles of Ctab For the Synthesis of Short Chain Esters

Reverse micelles formed using the cationic surfactant, hexadecyltrimethylammoniumbromide (CTAB) were applied as a reaction medium for esterification and transesterification (alcoholysis) reactions catalysed by a recombinant lipolytic enzyme, cutinase. Reactions were initially studied with either the alcohol substrate (acyl acceptor) or chloroform as cosurfactant. Chloroform was inhibitory to the enzyme. The enzyme demonstrated specificity towards short chain substrates and these were used to study some relevant parameters affecting specific activity. Optimum values for Wo, buffer concentration, pH, CTAB concentration and temperature were identified. Optimum value for Wo was the same for the two reactions whereas the effects of buffer concentration, pH, CTAB concentration and temperature were different. An optimum level of alcohol was identified, this was the same for both reaction types but the value was dependent on the alcohol. Fatty acids showed inhibitory effects at comparatively low concentrations co.
Simulating Source‐Limited and Sink‐Limited Kernel Set with CERES‐Maize

CERES-Maize simulates kernel set as a source-limited process based on average plant growth rate during the lag phase after flowering. Yet the number of kernels formed by maize (Zea mays L.) also depends on timely interaction between male and female flowers, which can limit formation of reproductive sinks under some conditions. Failure to account for sink-limited kernel set may contribute to simulation error observed under conditions that affect dynamics of pollen shed or silking, but do not alter crop growth rate. We developed algorithms for a Flowering Model to simulate sink-limited kernel set from flowering dynamics. This model was calibrated against kernel production in hybrid seed production fields and then linked to CERES-Maize. The Modified CERES-Maize was calibrated against two years of field data involving three hybrids, eight population densities, and seven N levels. Integrating the capacity to simulate sink-limited kernel set with source-limited kernel set increased simulation accuracy dramatically, relative to original CERES-Maize. For 13 commercial fields tested, Modified CERES-Maize decreased simulation error for kernels per plant from 17.1 to 2.3%, improved r 2 between measured and simulated values from 0.77 to 0.87, and decreased simulation error indicators mean error, root mean square error, and mean square deviation by 85, 40, and 64%, respectively. Modified CERES-Maize accounts for a much greater range of variability in the biological processes controlling kernel set.
Comment on “A Study of the F2 Layer Stratification on Ionograms Using a Simple Model of TIDs” by Jiang et al.

A paper by Jiang et al. (2019, https://doi.org/10.1029/2018JA026040) apparently tries to find common ground between observations of the F3 layer and TIDs by referring to the later when observed near the equatorial anomaly peak as a new type 1 and TIDs by using ray tracing models with arbitrarily adjusted TID parameters. Further confusion in conflating the two separate phenomena is created by referring to both as “stratification. ” This comment points out that the F3 is a daytime temporary layer maintained by E × B uplift at the magnetic equator, normally occurs consistently within the peaks of the equatorial ionization anomaly in F2 electron density and may last for hours. Such a layer can be described as a stratification. How this effect dies out beyond the anomaly peak has yet to be experimentally ascertained. A cusp or inflection type TID is a manifestation of a gravity wave with no consistency in location and local time, of short duration measured in minutes, involving a continuous descent in height and should not be described as stratification. The theory of both is well known, and attempting to explain a possible F3 layer as a new type of layer when seen in the vicinity of an equatorial anomaly peak misunderstands the theory of the phenomenon. Attempts to characterize such a phenomenon as a TID manifestation are not defensible.
Influência da adubação nitrogenada e potássica na eficiência do uso da água e na qualidade de frutos de melão

The present work was developed with the objective of studying 3 combined doses of N and K2O, applied as cover dressings within 15, 30 and 45 days after seedling emergence, in the water efficience use and fruit quality of muskmelons grown on a Dark Red Podzolic soil. The experiment was carried in Ilha Solteira, Sao Paulo State, Brazil. The experiment was carried out in 1991 with the Valenciano Amarelo and Eldorado 300 cultivars, in a 2.0 x 0.75m spacing. The results showed that N and K2O influenced some parameters of the Eldorado 300 cultivar: peel weight, seed weight and acidity; pulp yield in Valenciano Amarelo cultivar.
Locating QTL for osmotic adjustment and dehydration tolerance in rice

Research was conducted to identify and map quantitative trait loci (QTL) associated with dehydration tolerance and osmotic adjustment of rice. Osmotic adjustment capacity and lethal osmotic potential were determined for 52 recombinant inbred lines grown in a controlled environment under conditions of a slowly developed stress. The lines were from a cross between an Indica cultivar, Co39, of lowland adaptation and a Japonica cultivar, Moroberekan, a traditional upland cultivar. The QTL analysis was conducted using single marker analysis (ANOVA) and interval analysis (Mapmaker/QTL). The measurements obtained and the QTL identified were compared to root traits and leaf rolling scores measured on the same lines. One major locus was associated with osmotic adjustment. The putative locus for osmotic adjustment may be homoeologous with a single recessive gene previously identified for the same trait in wheat. The putative osmotic adjustment locus and two of the five QTL associated with dehydration tolerance were close to chromosomal regions associated with root morphology. In this population, osmotic adjustment and dehydration tolerance were negatively associated with root morphological characters associated with drought avoidance. High osmotic adjustment and dehydration tolerance were associated with Co39 alleles and extensive root systems were associated with Moroberekan alleles. To combine high osmotic adjustment with extensive root systems, the linkage between these traits will need to be broken. Alternatively, if the target environment is a lowland environment with only
CHARACTERIZATION OF BITUMINOUS CONCRETE USING FLYASH AS FILLER

A bituminous concrete used in pavements is a mixture of fine aggregate, coarse aggregate, filler material and bitumen as binder mixed in required amount to obtain a durable and strong mix to safely take load of traffic on it. Generally, crushed stone dust and cement are used as filler. Study has been conducted to understand and analyze the use of fly ash, a byproduct of thermal power plants in paving mix of bitumen. For the purpose of comparison, control mix with stone dust is also considered. Marshall test is considered as a means for the design of the mix and evaluating the paving mixes. It is found that the mix with fly ash as filler have inferior characteristics as compared to control mixes, but satisfies the desired criteria by higher margin. Therefore, it may be recommended to utilize fly ash wherever available which will result not only in reduction of execution cost, but also solve partly the disposal problems of such by-products.
Application of Regression Models in Bird Population Data: An Example of Haçlı Lake

In this study, the effects of habitat, ordo, UTM frame, seasons and number of species on bird populations and distribution in Hacli Lake were investigated. Bird population data were obtained using point counts and transect observation methods. Poisson regression is typically used in such data sets. The basic principle of Poisson regression assumes that the variance is equal to the mean. Failure to achieve this equality causes incorrect parameter estimates and standard errors. In practice, the variance is often higher than the mean (variance > mean). This is called over-dispersion, where the value of over-dispersion is greater than 1.0. The population status of the data set used in the study was over-dispersed. Negative binomial regression is the most common method used to eliminate the over-dispersion effect. In this case, the preferred method is the negative binomial regression method. The over-dispersion value in the Poisson regression was considerably greater than 1.0 (54.937) while the over-dispersion value was very close to 1.0 (1.588) in the negative binomial regression. The results indicated that the use of negative binomial regression method is more appropriate. Therefore, parameter estimations were interpreted according to negative binomial regression method. Herein, climatic factors including temperature and humidity exhibited significant impacts on population density and number of species.
Using photovoice as an arts-based method for grieving: LGBTQ + students and the pulse nightclub shooting

Abstract Given the increase of gun violence in the United States, teachers are left with the added obligation of helping students process traumatic events. The present study seeks to address the following questions: What are some ways in which students process grief through arts-based methods? What can we observe through photovoice, a community-based method that uses photography, about the perceptions of six LGBTQ + students at a predominantly white institution after the Pulse Massacre? The study consisted of semi-structured focus group interviews with these students, all of whom spent a week creating photos in response to the tragedy. In order to understand the depth of how stories may have oppositional counter-stories, we look to narrative inquiry and find intersectionality and colorblind intersectionality at play. Implications for practice, policy, and research are included.
A pentatricopeptide repeat gene of rice is required for splicing of chloroplast transcripts and RNA editing of ndhA

The pentatricopeptide repeat (PPR) family has been reported to be involved in many post-transcriptional processes, including RNA editing and RNA splicing in both chloroplasts and mitochondria. There are a large number of PPR proteins in plant genome, however, the biological functions of most PPR proteins have not yet been determined. We describe the involvement of a new PPR member (OsPPR4) for the splicing and RNA editing of chloroplast transcripts. The rice P-class PPR protein, OsPPR4, possesses an RNA recognition motif (RRM) and 15 PPR motifs. A retrotransposon (Tos17)-insertion mutant, osppr4, showed an albino phenotype with early seedling lethality. The same results were obtained from plants transformed with an OsPPR4 RNA interference (RNAi) construct. Disruption of OsPPR4 expression led to a strong defect in the splicing of atpF, ndhA, rpl2, and rps12-2 introns and influences the splicing of petB and rps16 introns. In addition, RNA editing of unspliced ndhA transcripts was decreased in the osppr4 line, whereas both spliced and unspliced ndhA were completely edited in WT plants. These results suggest that OsPPR4 is an essential factor for greening in plants and plays a non-redundant role in post-transcriptional regulation of chloroplast genes.
Assemblages of Amphibians, Reptiles, and Mammals on an Urban Military Base in Oklahoma

Abstract We surveyed Tinker Air Force Base, Oklahoma County, Oklahoma, an urban complex largely dominated by industrial and housing facilities but with habitats for herpetofauna and mammals. Using a variety of sampling techniques, we captured or observed >2,000 organisms on 16 study areas. We tallied 11 species of amphibians (all anurans), 26 species of reptiles, and 24 species of mammals, including several species of conservation concern. Species richness and diversity were predictably higher on green-space areas and were lower on areas surrounding airfield runways. Resumen Muestreamos a Tinker Air Force Base, condado de Oklahoma, Oklahoma, un complejo urbano mayormente dominado por las instalaciones industriales y de viviendas, pero con hábitats para herpetofauna y mamíferos. Usando una variedad de técnicas de muestreo, capturamos u observamos >2,000 organismos en 16 áreas de estudio. Registramos 11 especies de anfibios (todos anuros), 26 especies de reptiles, y 24 especies de mamíferos, incluyendo varias especies de interés para la conservación. La riqueza de especies y diversidad fueron previsiblemente mayores en las áreas de espacio verde y fueron menores en las áreas circundantes de las pistas aéreas.
Les peptides modulateurs du comportement alimentaire : espoirs et limites pour le traitement de l'obésité

Un bilan energetique positif lie a la fois a la diminution de l'activite physique et a une prise de nourriture exageree et/ou desequilibree est a l'origine d'une prise de poids excessif. Le nombre d'obeses est en augmentation constante depuis trente ans et la recherche de nouveaux traitements des obesites est primordiale pour juguler ce probleme a la fois de sante publique et economique. Les recepteurs de nombreux neuropeptides hypothalamiques agissant sur le comportement alimentaire constituent des cibles potentielles pour ces traitements. Ils sont retrocontroles par des hormones produites au niveau du tissu adipeux et du tractus gastro-intestinal. L'utilisation de leptine, une hormone anorexigene produite surtout par le tissu adipeux, n'a pu etre generalisee en raison du developpement d'une leptino-resistance chez le sujet obese. De meme, l'utilisation d'antagonistes du plus puissant peptide orexigene, le neuropeptide Y (NPY), n'a pas fourni les resultats escomptes en raison de la multiplicite et de la redondance des circuits regulant la prise de nourriture. Une multi-therapie ciblant simultanement d'autres voies de regulation devra donc etre envisagee afin d'obtenir des resultats significatifs et durables. Le PYY 3-36 a ete teste chez l'Homme. Des antagonistes d'autres peptides orexigenes (hormone de melanoconcentration, orexines.) ont ete testes avec un certain succes chez l'animal. Certaines approches de therapie genique au niveau central chez le Rat ont ravive les espoirs mis dans la leptine ou le NPY. Selon le type d'obesite, ces nouveaux traitements pourraient etre associes d'une part, a des molecules agissant sur les voies serotoninergiques et catecholaminergiques ou a des antagonistes des cannabinoides deja disponibles (ou prets de l'etre) sur le marche et d'autre part, a la chirurgie. Leur generalisation n'est cependant pas envisageable dans un futur immediat. Des changements comportementaux (nourriture, exercice) et des actions de prevention conduisant a une certaine hygiene alimentaire et comportementale pendant les phases precoces de la vie (in utero, petite enfance) resteront encore pour quelque temps le moyen le plus sur de limiter une prise de poids excessif. Les nouveaux traitements devraient pouvoir aider les obeses a mettre en place et garder ces changements de comportement et augmenter leurs chances de succes en les rendant plus efficaces.
Digestible fibre to ADF ratio and starch level in diets for growing rabbits

Abstract To evaluate the effect of digestible fibre (DF, hemicelluloses+pectins)/ADF ratio (1.0 and 1.3) and starch level (12, 15, and 18%) on health status, digestive physiology, growth performance, and carcass traits, 246 rabbits weaned at 27 d were fed until slaughter (76 d) with six diets formulated according to a bifactorial arrangement (2 DF/ADF ratios by 3 starch levels). Increasing DF/ADF improved DM digestibility (P<0.01), but did not affect growth performance, caecal content characteristics and slaughter traits. Increasing starch level improved digestibility efficiency and conversion index (P<0.001), decreased ileal mucosa villi height (P=0.04), without affecting growth performance, caecal fermentation and slaughter results. The increase of DF/ADF ratio tended to reduce mortality (25.0 vs 17.6%; P=0.11), whereas the raise of starch greatly increased mortality (from 6.9 to 43.1%; P<0.001) and sanitary risk (from 13.9 to 63.9%; P<0.001).