Miracle-dz/UniFAR-FTUs huggingface dataset:
Regulación del tráfico intracelular de la actividad Quitín Sintasa III en Saccharomyces cerevisiae

[ES] La pared celular de levaduras esta compuesta por una serie de capas, entre las cuales se encuentra la quitina. Este compuesto, a pesar de ser minoritario, es esencial para la viabilidad de Saccharomyces cerevisiae. Chs3 es la subunidad catalitica de la actividad Quitin Sintasa III (QSIII) y es responsable de la sintesis de la mayor parte de la quitina presente en la pared celular. Esta proteina presenta una regulacion muy compleja que va a permitir que se localice en el cuello de la levadura durante momentos muy discretos del ciclo celular. Dicha regulacion tiene lugar a nivel a nivel postraduccional y es mediada por una serie de proteinas que se han denominado Chs (de Chitin synthesis). En ausencia de las mismas Chs3 es incapaz de alcanzar la membrana plasmatica, quedando retenida en diferentes compartimentos intracelulares de la ruta de secrecion dependiendo del mutante. Esta regulacion tan compleja de Chs3 ha hecho que haya sido ampliamente estudiada como modelo del trafico intracelular regulado de proteinas. En este trabajo nos propusimos como primer objetivo profundizar en el estudio de la estructura-funcion de los distintos dominios de Chs3. Hemos visto que Chs3 es una proteina capaz de formar dimeros en el reticulo endoplasmatico a traves de su extremo N-terminal. La adquisicion de la estructura cuaternaria por parte de Chs3 es un proceso altamente regulado, de manera que los monomeros que se escapan del reticulo endoplasmatico son devueltos al mismo en vesiculas COPI, participando la proteina Rer1 en este sistema de control de calidad. Ademas, la oligomerizacion de Chs3 es una conformacion importante para la correcta cristalizacion de la quitina. Por otra parte, el dominio C-terminal desempena tres funciones: es necesario para la interaccion con la subunidad Chs6 del exomero, una maquinaria necesaria para la salida de Chs3 desde el trans-Golgi Network (TGN) hacia la membrana plasmatica; media el retorno de los monomero de Chs3 desde el cis-Golgi hacia el reticulo endoplasmatico en vesiculas COPI; y es imprescindible para la propia actividad catalitica. Finalmente, el dominio globular central, donde reside el centro catalitico, tambien esta implicado en la regulacion del trafico intracelular de Chs3. Un segundo objetivo ha sido profundizar en la funcion de Chs4, la proteina que activa y localiza a Chs3 en la membrana plasmatica. Trabajo previo en el laboratorio habia demostrado que Chs4 estabiliza a Chs3 en el cuello de la membrana plasmatica impidiendo su endocitosis. En este trabajo hemos descrito que esto es posible gracias a la existencia de un compartimento en el cuello, en el cual reside Chs3 y donde otras proteinas de la membrana plasmatica que no estan polarizadas se encuentran excluidas. Este compartimento esta definido por dos anillos concentricos, correspondientes con el anillo de septinas y un anillo constituido por la maquinaria endocitica que se dispone perifericamente al primero y en forma de parches. Chs3 seria transportada de forma polarizada hacia la region de membrana…
The safety and efficacy of vandetanib in the treatment of progressive medullary thyroid cancer

ABSTRACT Introduction: Traditional therapies for advanced or metastatic progressive medullary thyroid cancer (pMTC) are poor effective. Several TKIs have been tested in clinical trials in pMTC patients. Areas covered: This paper reviews efficacy and safety of vandetanib in the treatment of pMTC. Expert commentary: Vandetanib (trade name CAPRELSA® [Vandetanib]) has been shown to improve progression-free survival (30.5 vs 19.3 months in controls) in pMTC patients. Vandetanib is approved by FDA and EMA for metastatic MTC in adults; in adolescents and children with metastatic or locally advanced MTC, vandetanib seems to be effective. The most common adverse events in vandetanib-treated patients are: diarrhea, rash, folliculitis, nausea, QTc prolongation, hypertension and fatigue. In patients with aggressive differentiated thyroid cancer, vandetanib has shown promising results. Further research is needed to determine the ideal targeted therapy, based on tumor molecular characterization and host factors, to obtain the best response in terms of survival and quality of life.
Maximum de vraisemblance non-paramétrique avec régions de censure. Application à un modèle biophysique de décompression

We consider a non-parametric Maximum Likelihood (ML) density estimation problem with censored observations for a biophysical model describing the production of nitrogen bubbles during deep-sea diving. The ultimate objective is to predict the bubble production associated with a diving profile, in order to prevent decompression sickness accidents. Observations correspond to quantized counts of bubbles circulating in the blood (right ventricule), called grades, for a series of divers that used various diving profiles, each diver having an individual parameter value for the biophysical model. Assuming that the relation between observed grades and volume of gaz produced is known, we estimate the distribution of the model parameters in the population of divers considered by maximum Travail partiellement financé par le projet DGA–RAPID “SAFE DIVE”
Beta-caryophyllene is a dietary cannabinoid

The psychoactive cannabinoids from Cannabis sativa L. and the arachidonic acid-derived endocannabinoids are nonselective natural ligands for cannabinoid receptor type 1 (CB1) and CB2 receptors. Although the CB1 receptor is responsible for the psychomodulatory effects, activation of the CB2 receptor is a potential therapeutic strategy for the treatment of inflammation, pain, atherosclerosis, and osteoporosis. Here, we report that the widespread plant volatile (E)-β-caryophyllene [(E)-BCP] selectively binds to the CB2 receptor (Ki = 155 ± 4 nM) and that it is a functional CB2 agonist. Intriguingly, (E)-BCP is a common constituent of the essential oils of numerous spice and food plants and a major component in Cannabis. Molecular docking simulations have identified a putative binding site of (E)-BCP in the CB2 receptor, showing ligand π–π stacking interactions with residues F117 and W258. Upon binding to the CB2 receptor, (E)-BCP inhibits adenylate cylcase, leads to intracellular calcium transients and weakly activates the mitogen-activated kinases Erk1/2 and p38 in primary human monocytes. (E)-BCP (500 nM) inhibits lipopolysaccharide (LPS)-induced proinflammatory cytokine expression in peripheral blood and attenuates LPS-stimulated Erk1/2 and JNK1/2 phosphorylation in monocytes. Furthermore, peroral (E)-BCP at 5 mg/kg strongly reduces the carrageenan-induced inflammatory response in wild-type mice but not in mice lacking CB2 receptors, providing evidence that this natural product exerts cannabimimetic effects in vivo. These results identify (E)-BCP as a functional nonpsychoactive CB2 receptor ligand in foodstuff and as a macrocyclic antiinflammatory cannabinoid in Cannabis.
The tail of a chaperonin: the C‐terminal region of Esctierichia coli GroEL protein

The active form of the KSP60 molecular chaperone of Escherichia coli, GroEL, is a pair of seven‐membered rings. We have used site‐directed mutagenesis to construct forms of the 547‐amino‐acid monomer truncated at the C‐terminus. We show here that forms that are 520 amino acids long or longer are close to being fully functional. Removing one further amino acid, however, results in a protein, GroEL519, which retains little function. This truncated form is metabolically stable but is not recovered from the cell in particle form. When synthesized at high levels, it prevents the normal assembly of GroEL547 present in the same cell. When synthesized at low levels, it can be included, probably at low molar ratios, in particles formed by assembly‐competent forms of GroEL. This can be seen as partial complementation of the temperature‐sensitive mutant groEL44. We conclude that amino acid 520 is cruical for particle assembly. GroEL516 has in vivo properties similar to those of GroEL519, but the still shorter form, GroEL504, appears to be inactive.
Conditions d'entrée non réfléchissantes pour le calcul des écoulements instationnaires turbulents compressibles à bas nombre de Mach

Les conditions aux limites presentent des difficultes particulieres pour les ecoulements compressibles subsoniques, pour lesquels l'imposition des variables peut introduire des reflexions non physiques des ondes acoustiques sortant du domaine de calcul. Ce probleme est particulierement aigu pour les conditions d'entree des ecoulements turbulents, en particulier en LES ou DNS. En effet, la generation de turbulence s'appuie sur la prescription du champ de vitesse en entree, ce qui peut conduire a une reflexion non physique des ondes
The traveling salesman problem with pickup, delivery, and ride‐time constraints

In the traveling salesman problem with pickup, delivery, and ride‐time constraints (TSPPD‐RT), a vehicle located at a depot is required to service a number of requests where the requests are known before the route is formed. Each request consists of (i) a pickup location (origin), (ii) a delivery location (destination), and (iii) a maximum allowable travel time from the origin to the destination (maximum ride‐time). The problem is to design a tour for the vehicle that (i) starts and ends at the depot, (ii) services all requests, (iii) ensures that each request's ride‐time does not exceed its maximum ride‐time, and (iv) minimizes the total travel time required by the vehicle to service all requests (objective function). A capacity constraint that may be present is that the weight or volume of the undelivered requests on the vehicle must always be no greater than the vehicle's capacity. In this article, we concurrently analyze the TSPPD‐RT with capacity constraints and without capacity constraints. We describe two mathematical formulations of the problem. These formulations are used to derive new lower bounds on the solution to the problem. Then, we provide two exact methods for finding the optimal route that minimizes the total travel cost. Our extensive computational analysis on both versions of the TSPPD‐RT shows that the proposed algorithms are capable of solving to optimality instances involving up to 50 requests. © 2015 Wiley Periodicals, Inc. NETWORKS, Vol. 67(2), 95–110 2016
Reduction in injury rates in nursing personnel through introduction of mechanical lifts in the workplace.

BACKGROUND Health care workers incur frequent injuries resulting from patient transfer and handling tasks. Few studies have evaluated the effectiveness of mechanical lifts in preventing injuries and time loss due to these injuries. METHODS We examined injury and lost workday rates before and after the introduction of mechanical lifts in acute care hospitals and long-term care (LTC) facilities, and surveyed workers regarding lift use. RESULTS The post-intervention period showed decreased rates of musculoskeletal injuries (RR = 0.82, 95% CI: 0.68-1.00), lost workday injuries (RR = 0.56, 95% CI: 0.41-0.78), and total lost days due to injury (RR = 0.42). Larger reductions were seen in LTC facilities than in hospitals. Self-reported frequency of lift use by registered nurses and by nursing aides were higher in the LTC facilities than in acute care hospitals. Observed reductions in injury and lost day injury rates were greater on nursing units that reported greater use of the lifts. CONCLUSIONS Implementation of patient lifts can be effective in reducing occupational musculoskeletal injuries to nursing personnel in both LTC and acute care settings. Strategies to facilitate greater use of mechanical lifting devices should be explored, as further reductions in injuries may be possible with increased use.
Role of IL-5 in Innate and Adaptive Immunity to Larval Strongyloides stercoralis in Mice1

Protective immunity to Strongyloides stercoralis infective larvae in mice has been shown to be dependent on IL-5 based on mAb depletion studies. The goal of this study was to determine the functional role of IL-5 during the innate and adaptive immune response to larval S. stercoralis in mice. In these studies, three strains of mice were used: wild-type C57BL/6J (WT), IL-5 knockout (KO), and IL-5 transgenic (TG). Innate responses to the larvae indicated that there was enhanced survival in the KO animals and decreased survival in the TG animals compared with WT. Furthermore, killing of larvae in TG mice was associated with eosinophil infiltration and degranulation. In studying the adaptive immune response, it was observed that immunization of KO mice did not lead to the development of protective immunity. Experiments were then performed to determine whether KO mice reconstituted with Abs or cells could then develop protective immunity. KO mice displayed protective immunity via a granulocyte-dependent mechanism following injection of purified IgM from immune wild-type animals. Immunity in KO mice could also be reconstituted by the injection of eosinophils at the time of immunization. These eosinophils did not participate in actively killing the challenge infection, but rather were responsible for the induction of a protective Ab response. We conclude that IL-5 is required in the protective immune response for the production of eosinophils, and that eosinophils were involved in larval killing during innate immunity and in the induction of protective Abs in the adaptive immune response.
The Relationship Between Sexual Abuse / Trauma and Eating Disorders

Deborah was fourteen years old when she entered a residential treatment facility for bulimia with anorexic symptoms, along with drug dependency. During her initial phase of treatment, she remembered a traumatic memory that had been repressed for years. When Deborah was on a family vacation with her family at age 11 years, she was sexually assaulted by a group of unknown predators. As she had repressed this memory, she had told no one about the event, and had received no social support or an outlet to express and heal her pain resulting from the trauma.
Conception optimale de centrales solaires à concentration : application aux centrales à tour et aux installations "beam down"

Depuis les annees quarante, la consommation energetique mondiale n'a cesse d'augmenter. Cette energie etant majoritairement d'origine fossile, il en resulte une augmentation globale de temperature terrestre. De ce fait, il est devenu urgent de reduire les emissions de gaz a effet de serre pour stopper le changement climatique. Dans ce contexte, le developpement de la production d'electricite a partir d'energie solaire concentree par voie thermodynamique est une solution prometteuse. Les efforts de recherche visent a rendre cette technologie plus efficace et plus competitive economiquement. Dans ce but, ce manuscrit presente une methode de conception optimale pour les centrales solaires a recepteur central. Elle tire parti des methodes developpees depuis de nombreuses annees par le groupe de recherche StaRWest, regroupant notamment des chercheurs des laboratoires RAPSODEE (Albi), LAPLACE (Toulouse) et PROMES (Odeillo). Couplant des algorithmes de Monte Carlo a hautes performances et des algorithmes stochastiques d'optimisation, le code de calcul implementant cette methode permet la conception et l'optimisation d'installations solaires. Il est utilise pour mettre en evidence les potentialites d'un type de centrales a recepteur central peu repandu : les centrales a reflecteur secondaire, egalement appelees centrales de type "beam down".
An international perspective of advanced practice nursing regulation.

BACKGROUND There is no common understanding about the role of the advanced practice nurse across the globe and there is wide variation in the regulation of advanced practice nursing roles as well as their educational, licensing and credentialing requirements. AIM The goal of this research was to examine the status of advanced practice nursing regulation globally. METHODS An online survey link was emailed to National Nursing Associations and nursing health policy makers worldwide from June to December 2011. Questions focused on regulation, education, scope of practice, and barriers and opposition. Analysis included frequency statistics and descriptive data for survey questions and content analysis for two open-ended questions. LIMITATIONS The survey was offered online and only in English. Therefore, technology and language barriers may have influenced the results. RESULTS There is wide variation in educational requirements, regulation and scope of practice of advanced practice nurses. The barriers to advanced practice nursing are often linked to the status of legislation and credentialing in specific jurisdictions. CONCLUSION A database of advanced practice nursing regulation and issues related to practice has the potential to become a valuable resource for individual countries. IMPLICATIONS FOR NURSING AND HEALTH POLICY Each country has unique challenges related to health policy for advanced practice nursing roles. International nursing organizations have established programmes for regulation development; however, a stronger focus on monitoring regulation and more effective dissemination of information about available supports may have a bigger impact on the development and revision of health policy related to advanced practice nursing.
The value of interdisciplinary pain management in complex regional pain syndrome type I: a prospective outcome study.

BACKGROUND Complex regional pain syndrome (CRPS) type I is a symptom complex of severe, chronic limb pain, often associated with allodynia, vasomotor, and sudomotor changes. Optimal management of this condition is not well understood. The role of a traditional, comprehensive pain management program with long-term follow-up has not been evaluated. OBJECTIVE To define the benefit of the interdisciplinary approach in patients with CRPS type I. DESIGN Prospective, case series, outcomes evaluation. METHODS Patients with a diagnosis of CRPS type I entering the University Pain Center's intensive, outpatient pain management program were enrolled in an objective assessment study through the duration of the program, with a follow-up of 2 years. This program involved 4 weeks of interdisciplinary management comprised of 20 sessions of physical therapy, 20 sessions of occupational therapy, 12 sessions of water therapy, 20 sessions of group psychotherapy, stellate ganglion blocks, and drug therapy. OUTCOME MEASURES Specific objective measurements of upper extremity function, sensation and strength over time, and functional status 2 years after program completion. RESULTS Upper extremity weight tolerance increased dramatically by 29-pounds (p<0.05). Function improved, with a 35 inch-pound gain in BTE (Baltimore Therapeutic Equipment) extension (p<0.005) and a 50 inch-pound increase in flexion (p<0.02). Jebsen-Taylor multifunctional testing (fine and gross motor skills) normalized from 72 to 48 seconds (p<0.04). Stable anxiety levels despite increased patient effort implied improved pain tolerance. At the 2-year follow up, 75% of the patients were employed. CONCLUSION Patients with CRPS type I may benefit from a 4-week outpatient pain management program emphasizing rehabilitation.
Research on DOA Estimation Based on Deep Learning for the Sea Battlefield

The traditional direction finding methods based on interferometer and spatial spectrum estimation occupy the mainstream of target localization. However, they strongly depend on the array manifold and are sensitive to array errors. The emerging technologies based on deep learning are data-driven, they are expected to compensate the disadvantages of the traditional methods efficiently. In addition, they exhibit better performance in universality and robustness. In this correspondence, the DOA (Direction of Arrival) estimation algorithms based on deep learning are discussed, including multilayer perceptron (MLP), convolutional neural network (CNN), convolutional recurrent neural network (CRNN), Auto-Encoder (AE) and so on. On this basis, the differences are studied as well. Finally, we summarize the challenges and propose the future developments.
Massive Intravenous Immunoglobulin Treatment in Women with Four or More Recurrent Spontaneous Abortions of Unexplained Etiology: Down‐Regulation of NK Cell Activity and Subsets

PROBLEM: The aims of this study were to investigate the efficacy of massive intravenous immunoglobulin (MIVIg) treatment for women with recurrent spontaneous abortion (RSA) of unexplained etiology, and to investigate changes in peripheral natural killer (NK) cell activity and subsets. METHOD OF STUDY: MIVIg treatment was performed in 18 pregnancies from 15 women with 4 or more consecutive RSA of unexplained etiology. NK cell activity and subsets were assessed in 8 of the pregnancies. RESULTS: 14 pregnancies resulted in live births and 4 resulted in abortions with chromosome abnormality. The pre‐infusion NK cell activity (mean±SD, 40.9±17.0%) at 4.4±0.5 weeks of gestation (GW) decreased to 15.0±7.9% at post‐infusion status (5.4±0.5 GW). Pre‐infusion percentages of CD56+CD16− cells (3.5±2.1%) and CD56+CD16− cells (16.8±8.8%) decreased to 3.0±2.2% and 11.1±6.9%, respectively, after MIVIg treatment. CONCLUSIONS: MIVIg treatment was effective in all 14 pregnancies from RSA women of unexplained etiology, excluding 4 abortions with chromosome abnormality. Peripheral NK cell activity and subsets were suppressed by MIVIg treatment.
Effect of prostaglandins F2alpha and E1 on local tissue Po2 and microflow of the brain cortex (cat).

The effects of prostaglandin F2 alpha (PGF2 alpha) and E1 (PGE1) on local tissue Po2 and microflow in the cat's brain surface were studied. Local tissue Po2 was polarographically measured with a multiwire surface electrode, and microflow measured by local hydrogen clearance method. The PGs were investigated by intracarotid (i.c.) infusions, single i.c. and intravenous (i.v.) injections. The i.c. infusions were made with dose of 3, 10 and 30 microgram/kg/min of PGF2 alpha and 0.1, 0.4 and 1.0 microgram/kg/min of PGE1. The i.c. application of PGF2 alpha led to a decrease in local tissue Po2 and tissue microflow. With the use of comparatively low doses (3 and 10 microgram) the effect appeared to be more specific, since SAP did not change significantly. Systemic (i.v.) injections of PGF2 alpha caused a significant drop in arterial pressure and decrease in tissue Po2. Studies with PGE1 always demonstrated a decrease in local tissue Po2 and microflow associated with a significant decrease in the SAP. No evidence for a specific effect was found. Suggestions for possible mechanism of action for these PGs are made.