Miracle-dz/UniFAR-FTUs huggingface dataset:
Foodborne Infections and Intoxications in Hyderabad India

Foodborne diseases are one of the health hazards and causes of morbidity and mortality in developing countries. In India there are no systematic studies to understand the types of foods involved and the etiological agent causing the disease. Therefore, a pilot study was proposed to investigate the food poisoning cases, undertaken by the Ronald Ross Institute of Tropical Diseases, which is a referral hospital for foodborne diseases in Hyderabad. Food and stool/rectal swabs of the patients affected were collected for microbiological examination. Odds ratio and 95% confidence interval were used to express the statistical significance of the differences. Epidemiological, environmental, and laboratory components indicated that Staphylococcus aureus was the etiological agent in most of the cases and in one case Salmonella spp. were the main cause of food poisoning. This study indicated the need to take up foodborne disease surveillance under the Indian context and to identify the common high-risk food commodities for microbial contamination and identification.
Automatic segmentation of right ventricle on ultrasound images using sparse matrix transform and level set

An automatic framework is proposed to segment right ventricle on ultrasound images. This method can automatically segment both epicardial and endocardial boundaries from a continuous echocardiography series by combining sparse matrix transform (SMT), a training model, and a localized region based level set. First, the sparse matrix transform extracts main motion regions of myocardium as eigenimages by analyzing statistical information of these images. Second, a training model of right ventricle is registered to the extracted eigenimages in order to automatically detect the main location of the right ventricle and the corresponding transform relationship between the training model and the SMT-extracted results in the series. Third, the training model is then adjusted as an adapted initialization for the segmentation of each image in the series. Finally, based on the adapted initializations, a localized region based level set algorithm is applied to segment both epicardial and endocardial boundaries of the right ventricle from the whole series. Experimental results from real subject data validated the performance of the proposed framework in segmenting right ventricle from echocardiography. The mean Dice scores for both epicardial and endocardial boundaries are 89.1%±2.3% and 83.6±7.3%, respectively. The automatic segmentation method based on sparse matrix transform and level set can provide a useful tool for quantitative cardiac imaging.
On the Capacity of Generalized Write-Once Memory with State Transitions Described by an Arbitrary Directed Acyclic Graph

The generalized write-once memory introduced by Fiat and Shamir (1984) is a q-ary information storage medium. Each storage cell is expected to store one of q symbols, and the legal state transitions are described by an arbitrary directed acyclic graph. This memory model can be understood as a generalization of the binary write-once memory which was introduced by Rivest and Shamir (1982). During the process of updating information, the contents of a cell can be changed from a 0-state to a 1-state but not vice versa. We study the problem of reusing a generalized write-once memory for T successive cycles (generations). We determine the zero-error capacity region and the maximum total number of information hits stored in the memory for T consecutive cycles for the situation where the encoder knows and the decoder does not know the previous state of the memory. These results extend the results of Wolf, Wyner, Ziv, and Korner (1984) for the binary write-once memory.
Critical decision making in severe head injury management

The management of severe head injury (SHI) remains a major challenge not only for neurosurgeons but also for all other health professionals involved in the care of trauma patients. Any trauma patient with SHI is at risk of further neurological deterioration if appropriate measures are not instituted from the start of his or her treatment. Secondary insults due to ischaemic, hypotensive, and metabolic or other causes are still common, even in the most advanced neurocritical care settings. Management controversies are widespread and few decision options can be supported by Class I evidence. This article attempts to provide an up-to-date review of the published recommendations that could help health professionals in their management of SHI.
Recent Progress in Light Sheet Microscopy for Biological Applications

The introduction of light sheet fluorescence microscopy (LSFM) has overcome the challenges in conventional optical microscopy. Among the recent breakthroughs in fluorescence microscopy, LSFM had been proven to provide a high three-dimensional spatial resolution, high signal-to-noise ratio, fast imaging acquisition rate, and minuscule levels of phototoxic and photodamage effects. The aforementioned auspicious properties are crucial in the biomedical and clinical research fields, covering a broad range of applications: from the super-resolution imaging of intracellular dynamics in a single cell to the high spatiotemporal resolution imaging of developmental dynamics in an entirely large organism. In this review, we provided a systematic outline of the historical development of LSFM, detailed discussion on the variants and improvements of LSFM, and delineation on the most recent technological advancements of LSFM and its potential applications in single molecule/particle detection, single-molecule super-resolution imaging, imaging intracellular dynamics of a single cell, multicellular imaging: cell–cell and cell–matrix interactions, plant developmental biology, and brain imaging and developmental biology.
Persuading end users to act cautiously online: a fear appeals study on phishing

Purpose The purpose of this paper is to test the protection motivation theory (PMT) in the context of fear appeal interventions to reduce the threat of phishing attacks. In addition, it was tested to what extent the model relations are equivalent across fear appeal conditions and across time. Design/methodology/approach A pre-test post-test design was used. In the pre-test, 1,201 internet users filled out an online survey and were presented with one of three fear appeal conditions: strong fear appeal, weak fear appeal and control condition. Arguments regarding vulnerability of phishing attacks and response efficacy of vigilant online information-sharing behaviour were manipulated in the fear appeals. In the post-test, data were collected from 786 internet users and analysed with partial least squares path modelling. Findings The study found that PMT model relations hold in the domain of phishing. Self-efficacy and fear were the most important predictors of protection motivation. In general, the model results were equivalent across conditions and across time. Practical Implications It is important to consider online information-sharing behaviour because it facilitates the occurrence and success of phishing attacks. The results give practitioners more insight into important factors to address in the design of preventative measures to reduce the success of phishing attacks. Future research is needed to test how fear appeals work in real-world settings and over longer periods. Originality/value This paper is a substantial adaptation of a previous conference paper (Jansen and Van Schaik, 2017a, b).
An empirical test of the accuracy of an attack graph analysis tool

– The purpose of this paper is to test the practical utility of attack graph analysis. Attack graphs have been proposed as a viable solution to many problems in computer network security management. After individual vulnerabilities are identified with a vulnerability scanner, an attack graph can relate the individual vulnerabilities to the possibility of an attack and subsequently analyze and predict which privileges attackers could obtain through multi-step attacks (in which multiple vulnerabilities are exploited in sequence). , – The attack graph tool, MulVAL, was fed information from the vulnerability scanner Nexpose and network topology information from 8 fictitious organizations containing 199 machines. Two teams of attackers attempted to infiltrate these networks over the course of two days and reported which machines they compromised and which attack paths they attempted to use. Their reports are compared to the predictions of the attack graph analysis. , – The prediction accuracy of the attack graph analysis was poor. Attackers were more than three times likely to compromise a host predicted as impossible to compromise compared to a host that was predicted as possible to compromise. Furthermore, 29 per cent of the hosts predicted as impossible to compromise were compromised during the two days. The inaccuracy of the vulnerability scanner and MulVAL’s interpretation of vulnerability information are primary reasons for the poor prediction accuracy. , – Although considerable research contributions have been made to the development of attack graphs, and several analysis methods have been proposed using attack graphs, the extant literature does not describe any tests of their accuracy under realistic conditions.
Outliers affecting cardiac troponin I measurement: comparison of a new high sensitivity assay with a contemporary assay on the Abbott ARCHITECT analyser

Background False-positive cardiac troponin (Tn) results caused by outliers have been reported on various analytical platforms. We have compared the precision profile and outlier rate of the Abbott Diagnostics contemporary troponin I (TnI) assay with their high sensitivity (hs) TnI assay. Methods Three studies were conducted over a 10-month period using routine patients’ samples. TnI was measured in duplicate using the contemporary TnI assay in Study 1 and Study 2 (n = 7011 and 7089) and the hs–TnI assay in Study 3 (n = 1522). Critical outliers were defined as duplicate results whose absolute difference exceeded a critical difference (CD = z x √2 x SDAnalytical) at a probability level of 0.0005, with one of the results on the opposite side of the decision limit to its partner. Results The TnI concentration at 10% imprecision (coefficient of variation) for the contemporary TnI assay was 0.034 µg/L (Study 1) and 0.042 µg/L (Study 2), and 0.006 µg/L (6 ng/L) for the hs–TnI assay. The critical outlier rates for the contemporary TnI assay were 0.51% (Study 1) and 0.37% (Study 2) using a cut-off of 0.04 µg/L, and 0% for the hs–TnI assay using gender-specific cut-offs. Conclusion The significant number of critical outliers detected using the contemporary TnI assay may pose a risk for misclassification of patients. By contrast, no critical outliers were detected using the hs–TnI assay. However, the total outlier rates for both assays were significantly higher than the expected variability of either assay. The cause of these outliers remains unclear.
Translationally regulated C/EBPβ isoform expression upregulates metastatic genes in hormone‐independent prostate cancer cells

C/EBPβ is a transcription factor regulating key biological processes including cellular growth and differentiation and its increased expression correlates with tumor invasiveness. Recently, the increased expression of C/EBPβ was reported in proliferative inflammatory atrophy of the prostate, associating with increased COX‐2 expression and androgen receptor (AR) downregulation.
Efeitos da terapia estro-raloxifeno sobre o endométrio de ratas

PURPOSE: to evaluate the effects of conjugated equine estrogens (CEE) and raloxifene (Ral), alone or combined, on the rat endometrium. METHODS: fifty-six adult rats were ovariectomized and randomly divided into seven groups: GCont (control); GCEE (CEE 50 µg/kg); GCEE/25 (CEE 25 µg/kg); GRal/0.75 (Ral 0.75 mg/kg); GRal/0.4 (Ral 0.4 mg/kg); GCEERal (50/0.75) - (CEE 50 µg/kg + Ral 0.75 mg/kg), and GCEE-Ral (25/0.4) - (CEE 25 µg/kg + Ral 0.4 mg/kg). The drugs were orally administered (gavage) for 21 consecutive days. At the end of the experiment, all animals were anesthetized and sacrificed. Fragments of uterus were removed, fixed in 10% formaldehyde and processed for paraffin inclusion. The histological sections were stained by HE and submitted to histomorphometric evaluation. The following parameters were analyzed: thickness of superficial epithelium and number of endometrial glands/mm2 and of blood vessels/mm2. The data were evaluated using ANOVA followed by the Turkey-Kramer test. RESULTS: in the GCont and only Ral treatment (GRal/0.75 and GRal/0.4) the endometrium showed signals of atrophy. In the groups treated with only CEE signs of endometrial proliferation were observed, mainly in group GCEE/50. Also, there was endometrial proliferation in the groups that received combined CEE and Ral (Ral GCEE (50/0.75) and GCEE-Ral (25/0.4)), but it was more intensive in the animals treated with isolated estrogen than in those that received combined estrogen and raloxifene. CONCLUSION: raloxifene may partially block the action of estrogen on the castrated adult rat endometrium.
Comparison of cardiac TnI outliers using a contemporary and a high-sensitivity assay on the Abbott Architect platform

Background Assays for cardiac troponin (cTn) have undergone improvements in sensitivity and precision in recent years. Increased rates of outliers, however, have been reported on various cTn platforms, typically giving irreproducible, falsely higher results. We aimed to evaluate the outlier rate occurring in patients with elevated cTnI using a contemporary and high-sensitivity assay. Methods All patients with elevated cTnI (up to 300 ng/L) performed over a 21-month period were assayed in duplicate. A contemporary assay (Abbott STAT Troponin-I) was used for the first part of the study and subsequently a high-sensitivity assay (Abbott STAT High-Sensitive Troponin-I) was used. Outliers exceeded a calculated critical difference (CD) (CD = z × √2 × SDAnalytical) where z = 3.5 (for probability of 0.0005) and critical outliers also were on a different side of the decision level. Results The respective outlier and critical outlier rates were 0.22% and 0.10% for the contemporary assay (n = 4009) and 0.18% and 0.13% for the high-sensitivity assay (n = 3878). There was no significant reduction in outlier rate between the two assays (χ2 = 0.034, P = 0.854). Fifty-six percent of outliers occurred in samples where cTn was an ‘add-on’ test (and was stored and refrigerated prior to assay). Conclusion Despite recent improvements in cTn methods, outliers (including critical outliers) still occur at a low rate in both a contemporary and high-sensitivity cTnI assay. Laboratory and clinical staff should be aware of this potential analytical error, particularly in samples with suboptimal sample handling such as add-on tests.
The Prostate 68 : 1707 ^ 1714 ( 2008 ) ImagingtheEffectsofCastrationonBoneTurnoverand Hormone-Independent ProstateCancerColonization of Bone

INTRODUCTION. Tumor populations may selectively colonize bone that is being actively remodeled. In prostate cancer patients, androgen deprivation directly inhibits tumor growth initially, whilst induced bone loss may facilitate tumor colonization of bone by androgeninsensitive cells. We have tested this hypothesis using a xenograft model of early growth of prostate cancer in bone. METHODS. PC3 cells transfected with Green fluorescent protein (GFP) were injected into castrated and non-castrated athymic mice via intrabial and intracardiac routes. In vivo tumor growth was monitored daily and animals sacrificed 6–9 days following initial GFP-based detection of tumors. Tumor bearing and contra-lateral non-tumor bearing tibias were analyzed extensively by micro-CT and histology/immunohistochemistry for the presence of tumor cells and the effects of tumor and/or castration on bone cells and bone structure evaluated. RESULTS. GFP-positive tumors in bone were visible from 12 days post-injection following intratibial injection, allowing tumors <1 mm diameter to be monitored in live animals. Castration did not affect tumor frequency, tumor volume, or time to initial appearance of tumors injected via intratibial or intracardiac routes. Castration decreased trabecular bone volume in all mice. Significant tumor-induced suppression of numbers of osteoblasts, coupled with increased numbers of activated osteoclasts, was evident in both intact animals and castrated animals. CONCLUSIONS. In vivo GFP imaging allows the detection of early tumor growth at intraosseous sites. Castration induces bone loss, but PC3-GFP cells are also capable of inducing bone remodeling in intact animals at early time points, independently of pre-existing castrationinduced alterations to bone. Prostate 68: 1707–1714, 2008. # 2008 Wiley-Liss, Inc.
Note on 'The calculation of the probability of detection and the generalized Marcum Q-function' (Mar 89 389-400)

Part 1) of the Corollary is analogous to Yuen’s lower bound for li j l where i and j are the indexes of codewords g , and g, of G(n), such that d ( g , , g , ) = m (cf. [12]). Part 2) of the Corollary is analogous to the upper bound for l i j l as given by Cavior in [3]. Remark: The binomial coefficient occurring in Part 1) of the Corollary is close to the Catalan number C, = ( 7 ) l f 1. In fact we have
International Journal of Health Care Quality Assurance Improvement attributes in healthcare: implications for integrated care

Purpose – Healthcare quality improvement is a key concern for policy makers, regulators, carers and service users. Despite a contemporary consensus among policy makers that integrated care represents a means to substantially improve service outcomes, progress has been slow. Difficulties achieving sustained improvement at scale imply that methods employed are not sufficient and that healthcare improvement attributes may be different when compared to prior reference domains. The purpose of this paper is to examine and synthesise key improvement attributes relevant to a complex healthcare change process, specifically integrated care. Design/methodology/approach – This study is based on an integrative literature review on systemic improvement in healthcare. Findings – A central theme emerging from the literature review indicates that implementing systemic change needs to address the relationship between vision, methods and participant social dynamics. Practical implications – Accommodating personal and professional network dynamics is required for systemic improvement, especially among high autonomy individuals. This reinforces the need to recognise the change process as taking place in a complex adaptive system where personal/professional purpose/meaning is central to the process. Originality/value – Shared personal/professional narratives are insufficiently recognised as a powerful change force, under-represented in linear and rational empirical improvement approaches.
Doping in sport and exercise: anabolic, ergogenic, health and clinical issues

The use of doping agents is evident within competitive sport in senior and junior age groups, where they are taken by non-elite as well as elite participants. They are also taken in non-sporting contexts by individuals seeking to ‘improve’ their physique through an increase in muscle and/or decrease in fat mass. While attaining accurate data on the prevalence of their use has limitations, studies suggest the illicit use of doping agents by athletes and non-athletes may be 1–5% in the population and greater than 50% in some groups; with the prevalence being higher in males. There is conclusive evidence that some doping agents are anabolic and ergogenic. There is also evidence that the use of doping agents such as anabolic androgenic steroids, growth hormone and other anabolic agents, erythropoietin and stimulants conveys considerable health risks that include, but are not limited to: cardiovascular disease, diabetes, cancer, mental health issues, virilisation in females and the suppression of naturally produced androgens in males. This review will outline the anabolic, ergogenic and health impacts of selected doping agents and methods that may be used in both the sporting and physique development contexts. It also provides a brief tabulated overview of the history of doping and how doping agents may impact upon the analyses of clinical samples.
Negative interference by rheumatoid factor in alpha-fetoprotein chemiluminescent microparticle immunoassay

Background Rheumatoid factor causes positive interference in multiple immunoassays. Recently, negative interference has also been found in immunoassays in the presence of rheumatoid factor. The chemiluminescent microparticle immunoassay is widely used to determine serum alpha-fetoprotein. However, it is not clear whether the presence of rheumatoid factor in the serum causes interference in the chemiluminescent microparticle immunoassay of alpha-fetoprotein. Methods Serum alpha-fetoprotein was determined using the ARCHITECT alpha-fetoprotein assay. The estimation of alpha-fetoprotein recovery was carried out in samples prepared by diluting high-concentration alpha-fetoprotein serum with rheumatoid factor-positive or rheumatoid factor-negative serum. Paramagnetic microparticles coated with hepatitis B surface antigen–anti-HBs complexes were used to remove rheumatoid factor from the serum. Results The average recovery of alpha-fetoprotein was 88.4% and 93.8% in the rheumatoid factor-positive and rheumatoid factor-negative serum samples, respectively. The recovery of alpha-fetoprotein was significantly lower in the rheumatoid factor-positive serum samples than in the rheumatoid factor-negative serum samples. In two of five rheumatoid factor-positive samples, a large difference was found (9.8%) between the average alpha-fetoprotein recoveries in the serially diluted and initial recoveries. Fourteen rheumatoid factor-positive serum samples were pretreated with hepatitis B surface antigen-anti-HBs complex-coated paramagnetic microparticles. The alpha-fetoprotein concentrations measured in the pretreated samples increased significantly. Conclusions It was concluded that the alpha-fetoprotein chemiluminescent microparticle immunoassay is susceptible to interference by rheumatoid factor, leading to significantly lower results. Eliminating the incidence of negative interference from rheumatoid factor should be an important goal for immunoassay providers. In the meantime, laboratorians must remain alert to the negative interference by rheumatoid factor, and in some cases, pretreat rheumatoid factor-positive samples with blocking or absorbing reagents.