Miracle-dz/UniFAR-FTUs huggingface dataset:
Ultraviolet Photoprocessing of Glycine Adsorbed on Various Space-Relevant Minerals

The discovery of amino acids such as glycine on meteorites and comets confirms the role of small bodies as transport and delivery vehicles of building blocks of life on Earth and possibly on other planetary bodies of our Solar System. Glycine is quite interesting because it is the simplest of the 20 biogenic amino acids, from which complex organic molecules might have originated in our evolved Solar System. To investigate the possible chemical evolution of this molecule in space, it is important to consider how the interaction with mineral matrices influences its photostability. Indeed, the presence of minerals can mediate the effects of electromagnetic radiation, catalyzing photoreactions, or protecting molecules against degradation. Such interactions are responsible for the preservation/degradation mechanisms of organic molecules in space environments. Laboratory simulations of UV processing may provide key insights into the survival of organic molecules in space environment and rocky surfaces, which is of particular relevance for current missions of sample return from asteroids, such as NASA OSIRIS-REx and JAXA Hayabusa 2, and in particular, upcoming space exploration missions on planetary surfaces, such as ESA-Roscosmos ExoMars 2022 and NASA Mars 2020. In this article, we report a laboratory study of UV irradiation of glycine adsorbed on various space relevant minerals: forsterite, antigorite, spinel, and pyrite. We monitored possible changes of glycine functional groups due to UV irradiation through in situ infrared (IR) spectroscopic analysis. Results show that degradation of glycine occurs with a half-life of 0.5–2 h depending on the mineral substrate. Appearance of new IR bands suggests the occurrence of catalytic reactions mediated by minerals and UV.
Case Report: Meningoencephalomyelitis of Unknown Etiology Manifesting as a Bilateral Cranial Polyneuropathy in 3 Dogs

A bilateral cranial polyneuropathy was the primary magnetic resonance imaging (MRI) finding in three medium to large breed dogs diagnosed with meningoencephalomyelitis of unknown etiology. All three dogs presented with a progressive history of vestibular ataxia with either central vestibular or multifocal central nervous system (CNS) neuroanatomical localization. Brain MRI revealed variable degree of bilateral enlargement and/or increased contrast enhancement of the optic, oculomotor, trigeminal, facial, and vestibulocochlear nerves, as well as enhancement of the orbital fissure (oculomotor, trochlear, ophthalmic branch of trigeminal, and abducens nerves). There was evidence of intracranial and cranial cervical meningeal contrast enhancement in all three dogs and of cervical spinal cord lesions in 2. In all cases, more cranial nerves were affected than indicated by neurological examination. Cerebrospinal fluid (CSF) analysis was consistent with a mononuclear pleocytosis in 2 cases and a mixed cell (predominantly lymphocytic) pleocytosis in 1 case. All dogs were treated with immune suppressing medications and showed clinical improvement, although some cranial nerve deficits were persistent at follow up 2 months later. These are the first known cases of MUE diagnosed ante-mortem in a canine population documenting bilaterally symmetrical lesions affecting multiple cranial nerves. While MUE is a common cause of non-infectious inflammatory disease in dogs, it likely encompasses more classifications than have previously been reported, and should remain a differential for dogs of all ages and sizes presenting with cranial nerve deficits.
Continuous, Lateralized Auditory Stimulation Biases Visual Spatial Processing

Sounds in our environment can easily capture human visual attention. Previous studies have investigated the impact of spatially localized, brief sounds on concurrent visuospatial attention. However, little is known on how the presence of a continuous, lateralized auditory stimulus (e.g., a person talking next to you while driving a car) impacts visual spatial attention (e.g., detection of critical events in traffic). In two experiments, we investigated whether a continuous auditory stream presented from one side biases visual spatial attention toward that side. Participants had to either passively or actively listen to sounds of various semantic complexities (tone pips, spoken digits, and a spoken story) while performing a visual target discrimination task. During both passive and active listening, we observed faster response times to visual targets presented spatially close to the relevant auditory stream. Additionally, we found that higher levels of semantic complexity of the presented sounds led to reduced visual discrimination sensitivity, but only during active listening to the sounds. We provide important novel results by showing that the presence of a continuous, ongoing auditory stimulus can impact visual processing, even when the sounds are not endogenously attended to. Together, our findings demonstrate the implications of ongoing sounds on visual processing in everyday scenarios such as moving about in traffic.
DNA Polymorphisms and Mutations of the Tumor Necrosis Factor-α(TNF-α) Promoter in Langerhans Cell Histiocytosis (LCH)

Langerhans cell histiocytosis (LCH) is a clonal proliferation of dendritic histiocytes expressing elevated levels of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-1 (IL-1), and leukemia inhibitory factor (LIF). The cause of the increased cytokine levels is unknown, but DNA sequence changes in promoters could alter expression. The TNF-α and IFN-γ promoter DNA sequences of 12 LCH patients were studied and compared with normal individuals by dideoxy fingerprinting and DNA sequencing. Functional consequences of polymorphic or mutated sequences were assessed by cloning altered and control promoter sequences into a luciferase reporter gene vector. Electrophoretic mobility shifts (EMSA) after binding of nuclear extracts from a macrophage cell line (U-937) by mutated promoters were compared with controls. Five of 12 LCH patients had alterations in the TNF-α promoter DNA sequence. None were found in the IFN-γ gene promoter. Of the 5 wit.
Identification and Functional Characterization of the GALACTINOL SYNTHASE (MoGolS1) Gene in Melissa officinalis Plants

Galactinol and rafinose accumulation in plants is associated with stressful environmental conditionssuch as cold, heat, or dehydration by the action of galactinols synthase (GolS) in the raffinosefamily of oligosaccharides biosynthetic pathway from UDP-galactose. Moreover, several reportsmentioned that GolS transcription is up regulated by various environmental stresses like cold, heat,dehydration. Therefore, to determine whether MoGolS1 was induced with the abiotic stress weanalyzed the expression pattern of the gene under various abiotic stresses like heat, cold, abscisicacid, sucrose and salt concentration in the lemon balm plants grown in standard MS medium. TheMoGolS1 gene was 981-bp in length encoding 326 amino acids in its sequence and shared 77 and76% sequence similarity with Arabidopsis thaliana galactinol synthase4 (AtGolS4) and AtGolS1genes respectively. The MoGolS1 gene was strongly expressed by the abiotic stress induced bysucrose, ABA or heat shock. It was also expressed in responses to cold, manitol and NaCl.MoGolS1 gene induction with various stresses may be possible for itscrucial function in abioticstress tolerance in plants, providing a good engineering target for genetic engineering.
Embodiment as a Paradigm for Understanding and Treating SE-AN: Locating the Self in Culture

There has been a growing call for sociologically engaged research to better understand the complex processes underpinning Severe and Enduring Anorexia Nervosa (SE-AN). Based on a qualitative study with women in Adelaide, South Australia who were reluctant to seek help for their disordered eating practices, this paper draws on anthropological concepts of embodiment to examine how SE-AN is experienced as culturally grounded. We argue that experiences of SE-AN are culturally informed, and in turn, inform bodily perception and practice in the world. Over time, everyday rituals and routines became part of participants’ habitus’, experienced as taken-for-granted practices that structured life-worlds. Here, culture and self are not separate, but intimately entangled in and through embodiment. Approaching SE-AN through a paradigm of embodiment has important implications for therapeutic models that attempt to move anorexia nervosa away from the body and separate it from the self in order to achieve recovery. Separating experiences—literally disembodying anorexia nervosa—was described by participants as more than the loss of an identity; it would dismantle their sense of being-in-the-world. Understanding how SE-AN is itself a structure that structures every aspect of daily life, helps us to understand the fear of living differently, and the safety that embodied routines bring. We conclude by asking what therapeutic treatment might look like if we took embodiment as one orientation to SE-AN, and focused on quality of life and harm minimization.
Automated objective dystonia identification using smartphone-quality gait videos acquired in clinic

Background: Dystonia diagnosis is subjective and often difficult, particularly when co-morbid with spasticity as occurs in cerebral palsy. Objective: To develop an objective clinical screening method for dystonia Methods: We analyzed 30 gait videos (640x360 pixel resolution, 30 frames/second) of subjects with spastic cerebral palsy acquired during routine clinic visits. Dystonia was identified by consensus of three movement disorders specialists (15 videos with and 15 without dystonia). Limb position was calculated using deep neural network-guided pose estimation (DeepLabCut) to determine inter-knee distance variance, foot angle variance, and median foot angle difference between limbs. Results: All gait variables were significant predictors of dystonia. An inter-knee distance variance greater than 14 pixels together with a median foot angle difference greater than 10 degrees yielded 93% sensitivity and specificity for dystonia. Conclusions: Open-source automated video gait analysis can identify features of expert-identified dystonia. Methods like this could help clinically screen for dystonia.
Unraveling Myelin Plasticity

Plasticity in the central nervous system (CNS) allows for responses to changing environmental signals. While the majority of studies on brain plasticity focus on neuronal synapses, myelin plasticity has now begun to emerge as a potential modulator of neuronal networks. Oligodendrocytes (OLs) produce myelin, which provides fast signal transmission, allows for synchronization of neuronal inputs, and helps to maintain neuronal function. Thus, myelination is also thought to be involved in learning. OLs differentiate from oligodendrocyte precursor cells (OPCs), which are distributed throughout the adult brain, and myelination continues into late adulthood. This process is orchestrated by numerous cellular and molecular signals, such as axonal diameter, growth factors, extracellular signaling molecules, and neuronal activity. However, the relative importance of, and cooperation between, these signaling pathways is currently unknown. In this review, we focus on the current knowledge about myelin plasticity in the CNS. We discuss new insights into the link between this type of plasticity, learning and behavior, as well as mechanistic aspects of myelin formation that may underlie myelin plasticity, highlighting OPC diversity in the CNS.
Analysing the Narrative and Promises of ‘Avoided Deforestation’ Implementation in Central Africa

SUMMARY Projects aimed at reducing emissions from deforestation and forest degradation (REDD+) have expanded in Central Africa following carbon certification standards, which were intended to demonstrate the feasibility of payments and rewards earned depending on a measured quantity of avoided deforestation. We used storytelling as a communication concept to analyse the narratives of five main certification standards that accompanied the implementation of REDD+ projects in Central Africa. Our analysis focuses on two storylines: the measurement of avoided deforestation, and payments or rewards. The examination of official documents disseminated by certification standards and the results from a survey of REDD+ stakeholders highlighted a gap between these promises and reality. Our findings show that carbon standards have diffused an idyllic view of REDD+, simplifying methods of measuring avoided deforestation and promising payments, co-benefits and sustainable development. Unkept promises result in disappointment and declining enthusiasm on the part of those involved in REDD+ projects at an early stage.
The evolution of complex life and the stabilization of the Earth system

The half-billion-year history of animal evolution is characterized by decreasing rates of background extinction. Earth's increasing habitability for animals could result from several processes: (i) a decrease in the intensity of interactions among species that lead to extinctions; (ii) a decrease in the prevalence or intensity of geological triggers such as flood basalt eruptions and bolide impacts; (iii) a decrease in the sensitivity of animals to environmental disturbance; or (iv) an increase in the strength of stabilizing feedbacks within the climate system and biogeochemical cycles. There is no evidence that the prevalence or intensity of interactions among species or geological extinction triggers have decreased over time. There is, however, evidence from palaeontology, geochemistry and comparative physiology that animals have become more resilient to an environmental change and that the evolution of complex life has, on the whole, strengthened stabilizing feedbacks in the climate system. The differential success of certain phyla and classes appears to result, at least in part, from the anatomical solutions to the evolution of macroscopic size that were arrived at largely during Ediacaran and Cambrian time. Larger-bodied animals, enabled by increased anatomical complexity, were increasingly able to mix the marine sediment and water columns, thus promoting stability in biogeochemical cycles. In addition, body plans that also facilitated ecological differentiation have tended to be associated with lower rates of extinction. In this sense, Cambrian solutions to Cambrian problems have had a lasting impact on the trajectory of complex life and, in turn, fundamental properties of the Earth system.
Spontaneously Occurring Small-Colony Variants of Staphylococcus aureus Show Enhanced Clearance by THP-1 Macrophages

Staphylococcus aureus is a common cause of chronic and relapsing infection, especially when the ability of the immune system to sterilize a focus of infection is compromised (e.g., because of a foreign body or in the cystic fibrosis lung). Chronic infections are associated with slow-growing colony phenotypes of S. aureus on solid media termed small-colony variants (SCVs). Stable SCVs show characteristic mutations in the electron transport chain that convey resistance to antibiotics, particularly aminoglycosides. This can be used to identify SCVs from within mixed-colony phenotype populations of S. aureus. More recently, populations of SCVs that rapidly revert to a “wild-type” (WT) colony phenotype, in the absence of selection pressure, have also been described. In laboratory studies, SCVs accumulate through prolonged infection of non-professional phagocytes and may represent an adaptation to the intracellular environment. However, data from phagocytic cells are lacking. In this study, we mapped SCV and WT colony populations in axenic growth of multiple well-characterized methicillin-sensitive and methicillin-resistant S. aureus strains. We identified SCVs populations on solid media both in the presence and absence of gentamicin. We generated stable SCVs from Newman strain S. aureus, and infected human macrophages with WT S. aureus (Newman, 8325-4) and their SCV counterparts (SCV3, I10) to examine intracellular formation and survival of SCVs. We show that SCVs arise spontaneously during axenic growth, and that the ratio of SCV:WT morphology differs between strains. Exposure to the intracellular environment of human macrophages did not increase formation of SCVs over 5 days and macrophages were able to clear stable SCV bacteria more effectively than their WT counterparts.
Gut Microbiota-Based Algorithms in the Prediction of Metachronous Adenoma in Colorectal Cancer Patients Following Surgery

Evaluating the risk of colorectal metachronous adenoma (MA), which is a precancerous lesion, is necessary for metachronous colorectal cancer (CRC) precaution among CRC patients who had underwent surgical removal of their primary tumor. Here, discovery cohort (n = 41) and validation cohort (n = 45) of CRC patients were prospectively enrolled in this study. Mucosal and fecal samples were used for gut microbiota analysis by sequencing the 16S rRNA genes. Significant reduction of microbial diversity was noted in MA (P < 0.001). A signature defined by decreased abundance of eight genera and increased abundance of two genera strongly correlated with MA. The microbiota-based random forest (RF) model, established utilizing Escherichia–Shigella, Acinetobacter together with BMI in combination, achieved AUC values of 0.885 and 0.832 for MA, predicting in discovery and validation cohort, respectively. The RF model was performed as well for fecal and tumor adjacent mucosal samples with an AUC of 0.835 and 0.889, respectively. Gut microbiota profile of MA still existed in post-operative cohort patients, but the RF model could not be performed well on this cohort, with an AUC of 0.61. Finally, we introduced a risk score based on Escherichia–Shigella, Acinetobacter and BMI, and synchronous-adenoma achieved AUC values of 0.94 and 0.835 in discovery and validation cohort, respectively. This study presented a comprehensive landscape of gut microbiota in MA, demonstrated that the gut microbiota-based models and scoring system achieved good ability to predict the risk for developing MA after surgical resection. Our study suggests that gut microbiota is a potential predictive biomarker for MA.
Quality of Life, Toxicity and Unmet Needs in Nasopharyngeal Cancer Survivors

Concerted research efforts over the last three decades have resulted in improved survival and outcomes for patients diagnosed with nasopharyngeal carcinoma (NPC). The evolution of radiotherapy techniques has facilitated improved dose delivery to target volumes while reducing dose to the surrounding normal tissue, improving both disease control and quality of life (QoL). In parallel, clinical trials focusing on determining the optimal systemic therapy to use in conjunction with radiotherapy have been largely successful, resulting in improved locoregional, and distant control. As a consequence, neoadjuvant chemotherapy (NACT) prior to definitive chemoradiotherapy has recently emerged as the preferred standard for patients with locally advanced NPC. Two of the major challenges in interpreting toxicity and QoL data from the published literature have been the reliance on: (1) clinician rather than patient reported outcomes; and (2) reporting statistical rather than clinical meaningful differences in measures. Despite the lower rates of toxicity that have been achieved with highly conformal radiotherapy techniques, survivors remain at moderate risk of persistent and long-lasting treatment effects, and the development of late radiation toxicities such as hearing loss, cranial neuropathies and cognitive impairment many years after successful treatment can herald a significant decline in QoL. Future approaches to reduce long-term toxicity will rely on: (1) identifying individual patients most likely to benefit from NACT; (2) development of response-adapted radiation strategies following NACT; and (3) anticipated further dose reductions to organs at risk with proton and particle therapy. With increasing numbers of survivors, many in the prime of their adult life, research to identify, and strategies to address the unmet needs of NPC survivors are required. This contemporary review will summarize our current knowledge of long-term toxicity, QoL and unmet needs of this survivorship group.
The development of the sino‐atrial region of the chick heart

The development of the interatrial septum and the relation of the sinus venosus and sino‐atrial valves to the interatrial septum was followed in chick hearts ranging from 50 hours of incubation to the adult. The following singificant facts were established. The interatrial septum first appears at about 50 to 55 hours of incubation. It fuses with the endocardial cushions of the atrio‐ventricular canal, typically in the last quarter of the fifth day. Secondary perforations appear in the septum at the time of closure of the interatrial foramen primum. These are retained until hatching, although they may persist in the adult heart. The sinus venosus, a sinus septum, and the sino‐atrial valves are retained as definite structures in the adult fowl heart. The pulmonary vein and the left precava as they enter the heart form a prominent addition to the inter‐atrial septum proper which I have designated as the pars cavo‐pulmonalis. The pulmonary orifice in the left atrium is provided with an extensive flap‐like valve. A prominent limbus of Vieussens is present in the right atrium. A well‐developed median muscular arch overlies the interatrial septum and a pair of lateral muoscular arches extend over the atria. Their position would seem to be significant in the closing septal perforations during atrial systole.
MK2 Is Required for Neutrophil-Derived ROS Production and Inflammatory Bowel Disease

Inflammatory bowel disease (IBD) is a chronic disease that is commonly accompanied by increased inflammatory responses and elevated reactive oxygen species (ROS) of the gastrointestinal tract. Here, we found that MAPK-activated protein kinase 2 (MK2) modulates ROS production and is required for dextran sulfate sodium (DSS)-induced IBD in the mouse model. Genetic ablation of MK2 in the myeloid lineage cells (MK2Lyz2−KO) protected against DSS-induced colitis injury. In response to DSS challenge, compared to MK2lyz2−WT mice, MK2Lyz2−KO mice exhibited less damage of epithelial and goblet cells, decreased generation of interleukin (IL)-6, tumor necrosis factor (TNF)-α, and ROS, as well as reduced Ki67-positive cells and concentrations of myeloperoxidase (MPO) in the intestinal epithelium. Furthermore, upon treatment with formylated peptide N-formyl-methionyl-leucyl-phenylalanine (fMLF), the generation of ROS was attenuated in MK2-deficient neutrophils, in which the phosphorylation of Akt and p38 MAPK was also reduced. Collectively, these findings indicated that MK2 is required for neutrophil-derived ROS production and IBD, and MK2 and ROS are promising therapeutic targets for IBD.