Miracle-dz/UniFAR-FTUs huggingface dataset:
Biomechanical evaluation of flexor tendon graft with different repair techniques and graft surface modification

The purpose of this study was to investigate the biomechanical properties of modified repair techniques for flexor tendon reconstruction and the effects of surface modification using carbodiimide‐derivatized synovial fluid plus gelatin (cd‐SF‐G), compared to the traditional repair techniques. The second and fifth digits from 16 canine forepaws were randomly divided into 4 groups: (1) traditional graft repairs (TGR group) including distal Bunnell repair and proximal Pulvertaft weave repair; (2) modified graft repairs (MGR group) including distal graft bony attachment repair and proximal step‐cut repair; (3) group TGR coated with cd‐SF‐G (TGR‐C group); and (4) group MGR coated with cd‐SF‐G (MGR‐C group). Digit normalized work of flexion (nWOF), ultimate failure strength, and stiffness were measured. The nWOF in MGR group was significantly less than TGR group (p < 0.05). The nWOF in groups treated with cd‐SF‐G was significantly less than their untreated counterparts (p < 0.05). Ultimate load to failure of the MGR‐C group was significantly greater than the TGR‐C group (p < 0.05), but no significant difference in stiffness was found between these two groups. The modified techniques cannot only improve tendon gliding abilities but can also improve breaking strength. Additionally, surface modification with cd‐SF‐G significantly decreased the work of flexion. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:731–737, 2015.
Type-C RNA Virus Gene Expression in Human Tissue

Partially purified fractions of human tissues have been analyzed by competition radioimmunoassay for the presence of two of the principle structural components of type-C RNA viruses, the major core protein (p27 to p30) and the major envelope glycopeptides (gp69/71). Screening of tissues was carried out by use of a heterologous assay system of 125I-labeled Rauscher murine virus p30 antigen and anti-RD 114 virus serum which was found to detect a class of interspecies determinants common to murine, feline, and primate viruses. A competitor with the same apparent affinity for antibody binding as that of purified viral core proteins was found in relatively high concentration in tissues from patients with systemic lupus erythematosus, in some neoplastic tissues, and also in normal human tissues. This competitor from a lupus spleen chromatographed on phosphocellulose and showed size fractionation during gel filtration similar to known p27 to p30 viral proteins. An immunologically reactive protein was also demonstrated by immunodiffusion and by immunoprecipitation of 125I-labeled human protein with anti-RD 114 p28 serum. Analysis of these human competitor proteins with homologous assay systems of viral core proteins and corresponding antisera showed that all, including the normal tissue extracts, appear similar to core proteins of known viruses, especially the RD 114 and woolly monkey species. A hypothesis suggested by these data is that many, if not all, humans harbor at least part of the genome of one or more type-C viruses, the properties of which are similar to those of viruses from other mammalian species, particularly primates.
Incorporating Vital Factors in Agile Estimation through Algorithmic Method

Agile methods have become the mainstream of software development due to their enriched practices. Some commonly used practices include collaborative development, meeting evolving requirements with working software, simple design etc. These methods address the problem of volatile requirements by applying above practices. Thus, these practices reduce cost of change at later stage of software development. These methods do not hold big upfront for early estimation of size, cost and duration due to uncertainty in requirements. It has been observed that agile methods mostly rely on an expert opinion and historical data of project for estimation of cost, size and duration. It has been observed that these methods do not consider the vital factors affecting the cost, size and duration of project for estimation. In absence of historical data and experts, existing agile estimation methods such as analogy, planning poker become unpredictable. Therefore, there is a strong need to devise simple algorithmic method that incorporates the factors affecting the cost, size and duration of project. It also provides the basis for inexperienced practitioners to estimate more precisely. In this paper, we are presenting the study of both traditional and agile estimation methods with equivalence of terms and differences. We investigated some vital factors affecting the estimation of an agile project with scaling factor of low, medium and high. Also, an algorithm Constructive Agile Estimation Algorithm (CAEA) is proposed for incorporating vital factors.
EFEITO DA LIBERAÇÃO MIOFASCIAL NA FLEXIBILIDADE DE QUADRIL EM INDIVÍDUOS PRATICANTES DE ATIVIDADE FÍSICA

A flexibilidade e muito importante para a saude do individuo, observando que ela e uma das capacidades fisicas responsaveis por uma maior eficiencia no movimento humano, dessa maneira, diversas formas de intervencao buscando a melhora da flexibilidade ja vem sendo estudadas. Nesse pressuposto, investigacoes sobre a eficiencia de tecnicas capazes de melhorar capacidades fisicas, entre elas, a flexibilidade, sao de grande relevância para profissionais da saude. O objetivo desse estudo busca avaliar o efeito da liberacao miofascial na flexibilidade de individuos praticantes de atividade fisica. O estudo caracteriza-se como descritivo, de abordagem quantitativa aplicada transversalmente. A amostra foi composta por 27 individuos sendo 16 homens (idade: 23 ± 4,77) e 11 mulheres (idade: 21 ± 3,49). A coleta de dados foi realizada no laboratorio de cineantropometria do Centro Universitario Catolica de Quixada – Unicatolica. Realizou-se avaliacao da massa corporal e flexibilidade, de maneira aguda. A normalidade dos dados se deu pelo teste de Kolmogorov-Smirnov. Com analise descritiva dos dados, e ainda, foi realizado teste t de student para amostras pareadas, com significância de p ≤ 0,05. Apos liberacao percebeu-se que houve melhoria semelhante na flexibilidade da perna direita tanto para o sexo masculino (p=0,085) como para o feminino (p=0,002). Ja na perna esquerda, o sexo feminino teve resultados significativos (p=0,005). Conclui-se que, a liberacao miofascial se mostrou efetiva na melhora da flexibilidade tanto em homens quanto em mulheres.
Detection of epstein‐barr virus DNA in formalin‐fixed paraffin‐embedded tissue of nasopharyngeal carcinoma using polymerase chain reaction and in situ hybridization

The polymerase chain reaction method for amplification of DNA in formalin-fixed, paraffin-embedded tissue sections was used to detect Epstein-Barr virus DNA in nasopharyngeal carcinomas from Japanese patients. Thirty-one cases of nasopharyngeal carcinoma and 8 cases of lymph node metastasis of nasopharyngeal carcinoma were studied. Detection rates of Epstein-Barr virus in various types of nasopharyngeal carcinoma according to the World Health Organization classification were as follows: 10 of 10 undifferentiated carcinomas, 8 of 13 nonkeratinizing carcinomas, and 5 of 7 keratinizing carcinomas. Eight lymph node metastases, for which the primary was positive for Epstein-Barr virus, also contained Epstein-Barr virus DNA. By in situ hybridization using a biotinylated Epstein-Barr virus probe, it was clearly demonstrated that Epstein-Barr virus DNA was localized in the nuclei of the neoplastic cells. The clinical features of nasopharyngeal carcinoma with or without Epstein-Barr virus were not different. These results demonstrate that nasopharyngeal carcinoma in Japanese patients is closely associated with Epstein-Barr virus infection, similar to nasopharyngeal carcinoma of other endemic and nonendemic areas.
xidative stress and mitochondrial damage in the pathogenesis of LS : New perspectives

This review attempts to reconcile the present dual view of the mechanisms operating in Amyotrophic Lateral Sclerosis (ALS). On one side, oxidative stress, mitochondrial damage and protein aggregation are considered as causative of the disease, as strongly supported by evidence obtained in models based on the expression of ALS-typical mutant SOD1. On the other hand, evidence from models expressing ALStypical mutations in RNA-binding proteins such as FUS and TDP43 indicate that mRNA (dys)metabolism is a major pathway in this disease. A critical analysis of existing literature suggests that there may be more than one point of intersection.
Reflectionless Adaptive RF Filters: Bandpass, Bandstop, and Cascade Designs

A class of frequency-reconfigurable input-reflectionless/absorptive RF/microwave filters is presented. They consist of tunable complementary-duplexer architectures that are composed of a main and an auxiliary channel with opposite filtering transfer functions. By loading the auxiliary channel with a reference-impedance resistor and by taking the output node of the main channel as the output terminal of the overall circuit, a filtering network of the same type of the main channel with theoretically perfect input-reflectionless behavior at all frequencies can be realized. This technique can be applied to design spectrally agile completely input-reflectionless filters with any kind of transfer function, such as low-pass, high-pass, and single/multiband bandpass/bandstop filters. The theoretical analysis of the first-order absorptive bandpass/bandstop filtering sections based on a coupling-matrix formulation is detailed. Furthermore, the synthesis of high-selectivity reflectionless filters either by cascading multiple first-order cells or using high-order channels in a single complementary duplexer is also described. For practical-demonstration purposes, frequency-tunable lumped-element and microstrip prototypes are manufactured and characterized. They correspond to first- and second-order bandpass/bandstop filters. In addition, their in-series cascade connection is used to implement a bandpass filter with spectrally controllable passband and out-of-band notches.
Release of Betaine and Dexpanthenol from Vitamin E Modified Silicone-Hydrogel Contact Lenses

Abstract Purpose: To develop a contact lens system that will control the release of an osmoprotectant and a moisturizing agent with the aim to reduce symptoms of ocular dryness. Materials and methods: Profiles of the release of osmoprotectant betaine and moisturizing agent dexpanthenol from senofilcon A and narafilcon B contact lenses were determined in vitro under sink conditions. Both types of lenses were also infused with vitamin E to increase the duration of drug release due to the formation of the vitamin E diffusion barriers in the lenses. The release profiles from vitamin E-infused lenses were compared with those from the control lenses. Results: Both dexpanthenol and betaine are released from commercial silicone hydrogel lenses for only about 10 min. Vitamin E loadings into contact lenses at about 20–23% can increase the release times to about 10 h, which is about 60 times larger compared to the control unmodified lenses. Conclusions: Vitamin E-loaded silicone hydrogel contact lenses released betaine and dexpanthenol in a controlled fashion.
Clinicopathological Correlates in a PRNP P102L Mutation Carrier with Rapidly Progressing Parkinsonism‐Dystonia

Parkinsonism‐dystonia is rare in carriers of the prion protein (PrP) gene (PRNP) proline‐to‐leucine substitution at codon 102 (P102L mutation). The severity and distribution of PrP deposition may influence the clinical presentation. The authors present such clinicopathological correlation in a 56‐year‐old man who had a PRNP P102L mutation associated with a phenotype characterized by rapidly progressing parkinsonism‐dystonia. The patient was studied clinically (videotaped examinations, brain magnetic resonance images [MRIs]) and was analyzed using molecular genetics (gene sequence analysis) and neuropathology (histology, immunohistochemistry) during his 7‐month disease course. The patient had parkinsonism, apraxia, aphasia, and dystonia, which progressed rapidly. Molecular genetic analysis revealed his PRNP P102L mutation carrier status. Brain MRIs revealed progressive, global volume loss and T2‐weighted/fluid‐attenuated inversion recovery hyperintensity in the neocortex and basal ganglia. Postmortem examination showed neuronal loss, gliosis, spongiform changes, and PrP deposition in the striatum. PrP immunohistochemistry revealed widespread, severe PrP deposition in the thalamus and cerebellar cortex. Based on the neuropathological and molecular‐genetic analyses, the rapidly progressing parkinsonism‐dystonia was correlated with nigrostriatal, thalamic, and cerebellar pathology.
A low power 4th order MASH switched-capacitor ΣΔ modulator using ultra incomplete settling

A discrete-time, switched-capacitor, MASH 2-2 4th order ΣΔ modulator, clocked with frequency of 1 GHz, was designed in a 65 nm CMOS technology. This modulator uses passive integrators based on the ultra-incomplete settling (UIS) concept. Electrical simulations show that the modulator achieves a peak SNDR of 66.8 dB, a peak SNR of 67.7 dB, an ENOB of 10.8 bits and DR of 70dB for a signal with a bandwidth of 10 MHz, while dissipating 1.5 mW from a 1.1 V power supply voltage, indicating that, a FOM of 42.7 fJ/conv. -step can be reached.
Dietary Issues in Recurrent Abdominal Pain

The term recurrent abdominal pain (RAP), based on the pioneering work of Apley and Naish, describes children who have chronic abdominal pain without an identifiable organic basis (1). Community-based studies worldwide show that 10% to 46% of children 4 to 16 years old meet the criteria for RAP (2–5). Based on other reports and data from our institution, RAP accounts for approximately 25% of visits to a pediatric gastroenterologist/nurse practitioner (4,6,7). The pediatric Rome III committee introduced the term abdominal pain–related functional gastrointestinal disorders (APFGIDs) to supersede the term RAP (8). APFGIDs include 4 phenotypic subtypes: functional abdominal pain (FAP), irritable bowel syndrome (IBS, essentially, FAP with changes in stooling pattern), functional dyspepsia, pain in the upper abdomen), and the relatively rare disorder, abdominal migraine (8). APFGIDs are associated with variable levels of symptoms and distress, ranging from episodic mild to severe abdominal pain, often disruptive to school and other activities. Evidence demonstrates that 30% to 66% of children with RAP experience pain similar to that of adults and meet the adult Rome criteria for IBS (9–12).
Design method of stable force control system using new resonance ratio control and instantaneous state observer

This paper proposes a stable force control system to achieve the force control of industrial robots. The proposed stable force control system is designed by using an I-P force control system and a resonance ratio control system considering the required interaction with the environment. To design the resonance ratio control system considering the contact with the environment, this paper analyzes the stability of the proposed resonance ratio control and the bandwidth of a disturbance observer. This paper designs the resonance ratio control system using an instantaneous state observer considering the results of the stability analysis. In addition, a stable force control system is designed based on the resonance ratio control system using an instantaneous state observer. In this paper, the effectiveness of the proposed force control system is confirmed by performing numerical simulations and experiments using an industrial robot arm.
Multiple mechanisms for the activation of human platelet aggregation by Staphylococcus
 aureus: roles for the clumping factors ClfA and ClfB, the serine–aspartate repeat protein SdrE and protein A

The ability of Staphylococcus aureus cells to induce platelet aggregation has long been recognized. However, despite several attempts to identify the mechanisms involved in this interaction, the nature of the bacterial receptors required remains poorly understood. Using genetic manipulation, this study for the first time provides clear evidence that several S. aureus surface proteins participate in the inter‐action with platelets. Mutants of S. aureus strain Newman lacking one or more surface proteins were tested for their ability to stimulate platelet aggre‐gation. This approach was complemented by the expression of a number of candidate proteins in the non‐aggregating Gram‐positive bacterium Lacto‐coccus lactis. S. aureus‐induced aggregation was monophasic and was dependent on the platelet receptor GPIIb/IIIa. The fibrinogen‐binding proteins, clumping factors A and B and the serine‐aspartate repeat protein SdrE could each induce aggregation when expressed in L. lactis. Although protein A expressed in L. lactis was not capable of inducing aggregation independently, it enhanced the aggregation response when expressed on the surface of S. aureus. Thus, S. aureus has multiple mechanisms for stimulating platelet aggregation. Such functional redundancy suggests that this phenomenon may be important in the pathogenesis of invasive diseases such as infective endocarditis.
Integrated Chinese and Western medicine treatment

objective to analyze the therapeutic effect of integrated traditional Chinese and Western medicine on acute exacerbation of senile chronic obstructive pulmonary disease.Method Select our home2013year7Month~2015year9advanced cancer patients admitted in the month108Example as a research object,divides patients into observation groups and control groups,isExample,ViewGroup of patients treated with integrated Chinese and Western medicine,The control group was treated with Western medical methods,Comparative analysis of the effects of both groups.result Observer Groupwith treatment effective rate higher than control group patients,difference is statistically significantCP<0.05. Conclusion combination of traditional Chinese and western medicine in elderly patients with chronic obstructive pulmonary diseasein the treatment of acute attack period of high value,Effective hardening effect,It is worth popularizingthe.
Lung tissue behavior in the mouse during constriction induced by methacholine and endothelin-1.

Recently, mice have been extensively used to investigate the pathogenesis of pulmonary disease because appropriate murine models, including transgenic mice, are being increasingly developed. However, little information about the lung mechanics of mice is currently available. We questioned whether lung tissue behavior and the coupling between dissipative and elastic processes, hysteresivity (eta), in mice would be different from those in the other species. To address this question, we investigated whether tissue resistance (Rti) and eta in mice would be affected by varying lung volume, constriction induced by methacholine (MCh) and endothelin-1 (ET-1), and high-lung-volume challenge during induced constriction. From measured tracheal flow and tracheal and alveolar pressures in open-chest ICR mice during mechanical ventilation [tidal volume = 8 ml/kg, frequency (f) = 2.5 Hz], we calculated lung resistance (RL), Rti, airway resistance (Raw), lung elastance (EL), and eta (= 2piRti/EL). Under baseline conditions, increasing levels of end-expiratory transpulmonary pressure decreased Raw and increased Rti. The administration of aerosolized MCh and intravenous ET-1 increased RL, Rti, Raw, and EL in a dose-dependent manner. Rti increased from 0.207 +/- 0.010 to 0.570 +/- 0.058 cmH2O.ml-1.s after 10(-7) mol/kg ET-1 (P < 0.01). After induced constriction, increasing end-expiratory transpulmonary pressure decreased Raw. However, eta was not affected by changing lung volume, constriction induced by MCh and ET-1, or high-lung-volume challenge during induced constriction. These observations suggest that 1) eta is stable in mice regardless of various conditions, 2) Rti is an important fraction of RL and increases after induced contriction, and 3) mechanical interdependence may affect airway smooth muscle shortening in this species. In mammalian species, including mice, analysis of eta may indicate that both Rti and EL essentially respond to a similar degree.
Acute Ballistic Muscle Stretching Inhibits Maximal Strength Performance

In the December 1998 issue of Research Quarterly for Exercise and Sport, we presented data showing that acute static stretching of the hip, thigh, and calf muscles before the performance of a one-repetition maximum lift (lRM) resulted in a decreased IRM for both knee flexion and knee extension (Kokkonen, Nelson, & Cornwell, 1998). Since then, we have had the opportunity to engage in dialogue with many individuals who were interested in discussing the implications and mechanisms that accompanied our finding. The foremost question has dealt with the type of stretching activity used in the study. Because static stretching was used, many people asked if the negative impact of stretching was present following ballistic stretching. Unfortunately, we could not answer this question with any precision. In the Kokkonen et al. (1998) study, the issue of ballistic stretching had been avoided, because most exercise physiology textbooks recommend against doing ballistic stretching (see deVries & Housh, 1994; Foss & Keteyian, 1998; Plowman & Smith, 1997; Powers & Howley, 2001; Robergs & Roberts, 1997). The basis behind these recommendations is that ballistic stretching increases the chance of muscle injury, because the athlete is trying to lengthen the muscle while the myotatic reflex is contract-
Recognition of Surrounding Environmental Based on Hand-Haptic Force

This paper describes a user-friendly system to recognize the surrounding obstacles around the elderly using the hand-haptic force feedback, to help the elderly walk safely. The user is able to attain the surrounding recognition by perceiving repulsive force generated in feedback while manipulating the joystick. To build up the system, a virtual potential fields corresponding to the distance and direction to the obstacle is employed. Through the experimental results, it is found that the practice time that the user learns basic operation of the system is sufficiently short. Furthermore, the user feels the sense of security while recognizing the surrounding information through the hand-haptic force
Evidence of hydrocarbon nanodrops in highly ordered stratum corneum model membranes[S]

The stratum corneum (SC), the top layer of skin, dictates the rate of both water loss through the skin and absorption of exogenous molecules into the body. The crystalline organization of the lipids in the SC is believed to be a key feature associated with the very limited permeability of the skin. In this work, we characterized the organization of SC lipid models that include, as in native SC, cholesterol, a series of FFAs (saturated with C16-C24 chains), as well as a ceramide bearing an oleate chain-linked to a very long saturated acyl chain [N-melissoyl-oleoyloxy hexacosanoyl-D-erythro-sphingosine (Cer EOS)]. The latter is reported to be essential for the native SC lipid organization. Our 2H-NMR, infrared, and Raman spectroscopy data reveal that Cer EOS leads to the formation of highly disordered liquid domains in a solid/crystalline matrix. The lipid organization imposes steric constraint on Cer EOS oleate chains in such a way that these hydrocarbon nanodroplets remain in the liquid state down to −30°C. These findings modify the structural description of the SC substantially and propose a novel role of Cer EOS, as this lipid is a strong modulator of SC solid/liquid balance.