Miracle-dz/UniFAR-FTUs huggingface dataset:
Constraining electroweakinos in the minimal Dirac gaugino model

Supersymmetric models with Dirac instead of Majorana gaugino masses have distinct phenomenological consequences. In this paper, we investigate the electroweakino sector of the Minimal Dirac Gaugino Supersymmetric Standard Model (MDGSSM) with regards to dark matter (DM) and collider constraints. We delineate the parameter space where the lightest neutralino of the MDGSSM is a viable DM candidate, that makes for at least part of the observed relic abundance while evading constraints from DM direct detection, LEP and low-energy data, and LHC Higgs measurements. The collider phenomenology of the thus emerging scenarios is characterised by the richer electroweakino spectrum as compared to the Minimal Supersymmetric Standard Model (MSSM) -6 neutralinos and 3 charginos instead of 4 and 2 in the MSSM, naturally small mass splittings, and the frequent presence of long-lived particles, both charginos and/or neutralinos. Reinterpreting ATLAS and CMS analyses with the help of SmodelS and MadAnalysis 5, we discuss the sensitivity of existing LHC searches for new physics to these scenarios and show which cases can be constrained and which escape detection. Finally, we propose a set of benchmark points which can be useful for further studies, designing dedicated experimental analyses and/or investigating the potential of future experiments.
Search for 
hep
 solar neutrinos and the diffuse supernova neutrino background using all three phases of the Sudbury Neutrino Observatory

A search has been performed for neutrinos from two sources, the $hep$ reaction in the solar $pp$ fusion chain and the $\nu_e$ component of the diffuse supernova neutrino background (DSNB), using the full dataset of the Sudbury Neutrino Observatory. The $hep$ search is performed using both a single-bin counting analysis and a likelihood fit. We find a best-fit flux that is compatible with solar model predictions while remaining consistent with zero flux, and set a one-sided upper limit of $\Phi_{hep} <30\times10^{3}~\mathrm{cm}^{-2}~\mathrm{s}^{-1}$ (90% credible interval). No events are observed in the DSNB search region, and we also set an improved upper bound on the $\nu_e$ component of the DSNB flux.
Microhabitat as a determinant of diversity: stream invertebrates colonizing leaf packs

SUMMARY 1. Macroinvertebrate colonization of artificial leaf packs of differing palatability to detritivores was measured in a low-order stream. 2. The most palatable leaf types—alder and young beech—were colonized mainly by detritivores and consumed rapidly, so that species diversity on the substrate remained low. In the case of the less palatable old beech and paper ‘leaves’, however, colonization was slower but species diversity approached that of the surrounding benthos. 3. After 1 week, species diversity in paper ‘leaf packs was equivalent to that of the benthos, but species composition was dissimilar. 4. I argue that species diversity of a patch of stream bed may be inversely related to the abundance or palatability of a given food resource, and that this relationship is maintained by the instability of the stream bed habitat, precluding a long-term community response to increased food availability. It may be, however, that the dispersal abilities of many aquatic insects allow a rapid response to the creation of novel habitats.
The effects of competitor density on aggressive behaviour and resource defence in a Poeciliid fish

Summary To test the hypothesis that competitor density and time spent at a food resource influences aggressive behaviour in male swordtail, Xiphophorus sp. , fish were kept at two densities (low: 18 fish m−3; high: 54 fish m−3) and aggressive behaviour was recorded with the time at the food resource used as a covariate. Competitor density is likely to affect the cost:benefit ratio of food defence. When density is low, there should be sufficient food for all individuals and intra-specific interactions are expected to be rare, while at high densities, increased intra-specific encounter rates mean that individuals may spend more time defending a resource than utilising it. Food resource defence should occur at intermediate densities. The frequency of aggression, i.e. bites, chases, and display behaviour was significantly positively influenced by time spent at the food resource (Bites: F1,31 = 8.186, P = 0.007; Chases: F1,31 = 6.439, P = 0.016; Displays: F1,31 = 4.435, P = 0.043) suggesting that food resource defence occurred. Competitor density had no effect on food resource defence and minimal effect on the frequency of aggression with only one type of aggressive behaviour, male–male displays, showing a difference between densities (F1,31 = 6.975, P = 0.013). This finding is suggested to be a result of the formation of dominance hierarchies in this species. Aggression from the dominant individual may be directed at only subordinates of the next dominance rank and subordinate behaviour may be restricted by status rather than immediate threat. In such a situation, aggression may be independent of competitor density.
Gradient Projection for Solving Quadratic Programs with Standard Simplex Constraints

An important method to optimize a function on standard simplex is the active set algorithm, which requires the gradient of the function to be projected onto a hyperplane, with sign constraints on the variables that lie in the boundary of the simplex. We propose a new algorithm to efficiently project the gradient for this purpose. Furthermore, we apply the proposed gradient projection method to quadratic programs (QP) with standard simplex constraints, where gradient projection is used to explore the feasible region and, when we believe the optimal active set is identified, we switch to constrained conjugate gradient to accelerate convergence. Specifically, two different directions of gradient projection are used to explore the simplex, namely, the projected gradient and the reduced gradient. We choose one of the two directions according to the angle between the directions. Moreover, we propose two conditions for guessing the optimal active set heuristically. The first condition is that the working set remains unchanged for many iterations, and the second condition is that the angle between the projected gradient and the reduced gradient is small enough. Based on these strategies, a new active set algorithm for solving quadratic programs on standard simplex is proposed.
Biological Monitoring and Bridging the Gap Between Land Management and Science

Abstract Traditional monitoring approaches may have the objective of gathering data to estimate population size for a given species, but often lack the context of hypothesis testing. As such, many natural areas managers (responsible for ecological monitoring) and scientists have shared little common ground. The goal of monitoring should be to inform managers so that they can insure that the species, communities, and ecosystems under their charge are able to persist in the face of stressors, often from anthropogenic sources. The inherent complexity of natural systems makes this a daunting task, a task that requires a renewed partnership between managers and conservation biologists. The non-equilibrium paradigm of population dynamics establishes a theoretical framework for shifting monitoring objectives from only population estimates to understanding the processes that drive the dynamics of those populations. Using a hypothesis-driven scientific approach, monitoring designs can embrace the scientific method, provide insights into the ecological processes at work within natural systems, and importantly, point directly to if, when, and how active management may need to be employed in order to prevent the loss of biodiversity. This approach provides a common ground for conservation biologists and natural areas managers to forge partnerships to better understand the complexity of ecological systems and our ability to sustain those systems for future generations.
Conditional mutualism between the giant kelp Macrocystis pyrifera and colonial epifauna

It is generally considered that colonial invertebrates living on the surfaces of macro- algae have a negative influence on the growth rates and survivorship of their substratum. To date no evidence has been provided from natural macroalgal/epifaunal associations to support this view. We investigated the influence of colonial bryozoans and hydrozoans on the growth rate and nitrogen physiology of their substratum, the giant kelp Macrocystis pyrifera, and the impact of seasonal changes of light and the seawater nitrogen concentration on this relationship. Throughout this study there was no evidence of any negative impact of colonization by either epifaunal group on the growth of M. pyrifera. During a period of low concentrations of inorganic nitrogen in seawater, the growth of M. pyrifera fronds heavily colonized by hydroid colonies was higher than less heavily colonized individuals. During this time hydroids could provide on average between 71 and 122% of the nitro- gen required for uptake by tagged M. pyrifera fronds at ambient seawater nitrogen concentrations. Evidence suggests that enhanced growth was caused by the provision of ammonium excreted by the hydroid colonies. Evolutionary pressure to limit the impact of colonization by sessile animals on macroalgae is likely to be exerted on both the algal substratum and the attached animal. We suggest that due to their close relationship this pressure may ultimately result in the development of mutualistic partnerships between algae and specialized sessile seaweed-dwelling fauna in natural associations.
Prediction of the Carcass Value of Young Holstein-Friesian Bulls Based on Live Body Measurements

Abstract The aim of this study was to determine the suitability of ultrasound and zoometric measurements and visual muscle scoring for predicting the carcass value of 167 young Holstein-Friesian (HF) bulls. Zoometric and ultrasound measurements were performed and live muscle scoring was estimated before slaughter. After slaughter, hot carcass weight (HCW) was determined and carcasses were assigned to conformation and fat classes according to the EUROP system. Multiple regression equations were derived to estimate the weight, conformation and fatness of carcasses. HCW was estimated using the following equations: Ŷ = 1.507x1 + 1.103x2 + 4.043x3 + 5.53x4 + 0.379x5 + + 8.076x6 - 678.93 (R2=0.892; Sy = 16.28) and Ŷ = 2.525x4 + 0.579x7 + 0.451x8 - 134.17 (R2=0.943; Sy = 11.84); independent variables x1 - height at sacrum (cm); x2 - chest girth (cm); x3 - pelvic width (cm); x4 - pelvic length (cm); x5 - thickness of M. gluteo-biceps (mm); x6 - intravital muscle scoring (points); x7 - thickness of M. longissimus dorsi (mm); x8 - live weight (kg). Validation of the first regression equation revealed overestimation of HCW by 1.25% on average, while validation of the second equation revealed its underestimation by 1.85% on average. It was found that intravital muscle scoring and selected ultrasound and zoometric measurements of HF bulls can be used in formulating regression equations for predicting the carcass value of live animals. The proposed models enable predicting the carcass value of young bulls with satisfactory accuracy, thus contributing to an objective live beef cattle assessment
Vocalisations of the seychelles sheath-tailed bat Coleura seychellensis

The vocalisations of the Critically Endangered Seychelles sheath-tailed bat Coleura seychellensis are described. Four call types are identified : complex social calls, orientation calls within the roost, open habitat orientation calls and foraging calls. The open habitat orientation calls and foraging calls are quasi-CF, the latter having alternating frequencies. The function of the alternating calls is discussed and it is concluded that in this species the lower frequency tone is used for navigation (being identical to the open habitat orientation calls) and the higher frequency tone for prey detection in cluttered environments.
Stabilization of α‐chymotrypsin by ionic liquids in transesterification reactions

Five different ionic liquids, based on dialkylimidazolium and quaternary ammonium cations associated with perfluorinated and bis (trifluoromethyl) sulfonyl amide anions, were used as reaction media to synthesize N-acetyl-L-tyrosine propyl ester by transesterification with alpha-chymotrypsin at 2% (v/v) water content at 50 degrees C. The synthetic activity was reduced by the increase in alkyl chains length of cations and by increases in anion size, which was related to the decrease in polarity. Incubation of the enzyme (with and without substrate) in ionic liquids exhibited first-order deactivation kinetics at 50 degrees C, allowing determination of deactivation rate constants and half-life times (1-3 h). Ionic liquids showed a clear relative stabilization effect on the enzyme, which was improved by increased chain length of the alkyl substituents on the imidazolium ring cations and the anion size. This effect was 10-times enhanced by the presence of substrate. For example, 1-butyl-3-methylimidazolium hexafluorophosphate increased the alpha-chymotrypsin half-life by 200 times in the presence of substrate with respect to the 1-propanol medium. These results show that ionic liquids are excellent enzyme-stabilizing agents and reaction media for clean biocatalysis in non-conventional conditions.
雷帕霉素对重型再生障碍性贫血模型小鼠脾脏CD4+CD25+ T细胞凋亡的影响

目的 探讨雷帕霉素（Rapamycin, RAPA）对重型再生障碍性贫血（SAA）模型小鼠CD4+CD25+ Treg细胞凋亡情况的影响及可能的机制。 方法 以近交系雌性BALB/c小鼠作为对照（对照组），应用IFN-γ腹腔注射联合白消安灌胃的方法建立SAA小鼠模型（SAA组），并使用RAPA腹腔注射5 d治疗SAA小鼠（RAPA组）。 骨髓活检病理学检查观察各组小鼠的骨髓造血组织变化；免疫磁珠分选出各组小鼠脾脏Treg细胞，用流式细胞术检测其凋亡率；Western blot法检测各组小鼠脾脏Treg细胞的Akt、磷酸化（p）-Akt、Stat3、p-Stat3的表达水平；收集各组小鼠外周血和脾脏中单个核细胞，用流式细胞术检测CD4+CD25+Foxp3+ Treg细胞的比例变化。 结果 与对照组相比，SAA组造血细胞明显减少，且造血细胞被大量的脂肪组织所代替，RAPA组造血细胞亦明显减少，可见大量脂肪细胞；对照组、SAA组、RAPA组小鼠胫骨骨髓造血组织面积分别为（94.25±4.20）%、（7.00±2.00）%、（9.75±1.83）%，差异有统计学意义（Welch F=1 441.822，P<0.001）；RAPA组略高于SAA组[Δx=2.15%（95%CI 0.15%~5.35%），P=0.037]。 对照组、SAA组、RAPA组脾脏Treg细胞凋亡率分别为（19.84±1.39）%、（29.85±2.72）%、（22.39±3.71）%，差异有统计学意义（F=18.338，P<0.001）；RAPA组Treg细胞凋亡率明显低于SAA组。 RAPA组脾脏Treg细胞中Akt、Stat3的表达水平高于SAA组，而p-Akt和p-Stat3表达水平低于SAA组（P值均<0.05）。 SAA组、RAPA组的脾脏CD4+CD25+Foxp3+/CD4+CD25+、CD4+CD25+Foxp3+/CD4+细胞比例均低于对照组，且RAPA组明显高于SAA组（P值均<0.017）。 结论 IFN-γ联合白消安诱导的SAA模型小鼠脾脏Treg细胞存在过度凋亡现象，而RAPA可能通过抑制Treg细胞内Akt及Stat3的磷酸化上调Foxp3的表达，从而抑制Treg细胞的凋亡。
采用全自动标准化方法检测正常人血浆VWF水平及其影响因素分析

目的 采用全自动标准化方法检测正常人血管性血友病因子（VWF）各指标并分析其影响因素。 方法 采用全自动、标准化检测仪及配套试剂测定70名正常人VWF抗原（VWF∶Ag）、VWF瑞斯托霉素辅因子活性（VWF∶Rco）、VWF胶原结合试验（VWF∶CB）、凝血因子FⅧ活性（FⅧ∶C），比较年龄、ABO血型、性别及地域因素对正常人VWF各指标的影响。 对英国国家生物标准与检定所（NIBSC）确认的8个标准血浆样本（2名正常人、6例2型VWD）进行VWF各项指标检测。 结果 ①70名正常人血浆VWF∶Ag为（95.4±44.9）%，VWF∶Rco为（105.9±35.4）%，VWF∶CB为（89.8±28.4）%；VWF∶Rco/VWF∶Ag比值为1. 18±0.25，VWF∶CB/VWF∶Ag比值为1. 03±0.29。 ②年龄≥30岁组与<30岁组比较，血浆VWF各指标差异均无统计学意义（P>0.05）。 ③O型血组VWF∶Rco、VWF∶CB低于非O型血组（t=2.074，P=0.042；t=3.949，P=0.001），VWF∶Ag、VWF∶Rco/VWF∶Ag、VWF∶CB/VWF∶Ag比值差异无统计学意义（P>0.05）。 ④男、女性组VWF各指标差异无统计学意义（P>0.05）。 ⑤北方（淮河以北地区）组VWF∶Ag、VWF∶CB高于南方（苏州地区）组（t=4.525，P=0.001；t=3.214，P=0.002），VWF∶Rco/VWF∶Ag、VWF∶CB/VWF∶Ag比值低于南方组（t=6.373，P=0.001；t=2.902，P=0.005），VWF∶Rco差异无统计学意义（t=1.598，P>0.115）。 ⑥8个标准血浆样本各项VWF指标检测结果符合已知诊断。 结论 通过使用全自动标准化检测设备首次获得较完整的正常人血浆VWF水平检测数据；ABO血型和地域因素对VWF水平有显著影响，而年龄和性别的影响不显著。
免疫抑制治疗后合并血流感染的重型再生障碍性贫血患者临床特征及预后分析

目的 分析抗胸腺细胞球蛋白（ATG）治疗后合并血流感染的重型再生障碍性贫血（SAA）患者的临床特征、近期疗效及预后。 方法 回顾性分析2000年1月至2011年7月行ATG治疗的264例SAA患者的临床特征，并分析合并血流感染患者的细菌学特征及其发生血流感染的危险因素，观察其近期（治疗后3、6个月）疗效，并进行远期预后分析。 结果 264例患者中有49例（18.6%）ATG治疗后出现血流感染。 49例患者中男31例，女18例，中位年龄20 (4~62）岁，其中极重型AA（VSAA) 38例，SAA 11例，治疗后至发生血流感染的中位时间为13 (2~233）d。 大肠埃希菌、铜绿假单胞菌和肺炎克雷伯杆菌为最常见的菌种，分别占28. 4%、20.9％及14. 9%。 23例（46.9%）患者合并耐药菌血流感染。 VSAA患者ATG治疗后血流感染发生率为28. 4%（38/134），显著高于SAA患者的8. 5%（11/130）（P<0.001）。 VSAA（RR=4.77，95%CI 1.97~11.52，P=0.001）、ATG治疗前1周仍合并感染（RR= 4.76，95%CI 2.05~11.11，P<0.001）是患者发生血流感染的危险因素。 ATG治疗后血流感染患者与无感染者比较，3个月血液学反应率分别为10. 6％及35. 6%（P<0.001），6个月血液学反应率分别为17. 0％及55. 6%（P<0.001）；5年OS率分别为36. 4%（95%CI 21.3%~51.5%）及74. 5%（95%CI 68.4%~80.7%）（P< 0.001）。 结论 ①VSAA患者ATG治疗后血流感染发生率高于SAA；②VSAA和ATG治疗前合并感染为发生血流感染的危险因素；③ATG治疗后合并血流感染患者较无感染者近期疗效及远期预后差。
TREM-1 and TREM-2 Expression on Blood Monocytes Could Help Predict Survival in High-Grade Glioma Patients

Objective In recent years, the role of the modern inflammatory markers TREM-1 (triggering receptors expressed on myeloid cells) and HMGB1 (high mobility group box 1 protein) in tumorigenesis has begun to be studied. Their role in gliomas is not clear. The aim of our study was to find the role of inflammation in gliomas. Patients and Methods. In 63 adult patients with gliomas and 31 healthy controls, the expressions of TREM-1 and TREM-2 on CD14+ blood cells (method: flow cytometry) and the levels of soluble sTREM-1, HMGB1, IL-6, and IL-10 (Elisa tests) were analyzed. Results Cox proportional hazard analysis showed that a TREM-1/TREM-2 ratio was associated with reduced overall survival (HR = 1.001, P = 0.023). Patients with a TREM-1/TREM-2 ratio above 125 survived significantly shorter than patients with a TREM-1/TREM-2 ratio below 125. The percentage of CD14+ TREM-1+ cells was strongly associated with a plasma IL-6/IL-10 ratio (positively) and with IL-10 (negatively). Conversely, we found a higher percentage of CD14+ TREM-2+ monocytes in better surviving patients; these cells could downregulate the exaggerated inflammation and potentiate the phagocytosis in the tumor. The serum levels of HMGB1 negatively correlated with the percentage of CD14+ TREM-1+ cells and with the TREM-1/TREM-2 ratio. The positive correlation between the serum levels of a late proinflammatory cytokine HMGB1 with the percentage of TREM2+ CD14+ monocytes can be explained as an effort for suppression of systemic inflammation by anti-inflammatory acting CD14+ TREM-2+ cells. Conclusion We showed that the TREM-1/TREM-2 ratio (expression on the surface of blood monocytes) could help predict prognosis in patients with gliomas, especially in high-grade gliomas, and that systemic inflammation has an impact on the patient's overall survival. This is the first study that showed that TREM expression on monocytes in peripheral blood could help predict prognosis in patients with gliomas.
60 mg·m−2·d−1柔红霉素联合标准剂量阿糖胞苷诱导治疗≤65岁初治急性髓系白血病患者的疗效和安全性分析

目的 观察60 mg·m−2·d−1柔红霉素(DNR)联合标准剂量阿糖胞苷(DA)方案诱导治疗≤ 65岁初治急性髓系白血病(AML)的长期疗效和安全性。 方法 回顾性分析116例2012年7月至2016年2月接受基于DNR 60 mg·m−2·d−1的DA诱导方案治疗且≤65岁初治非急性早幼粒细胞白血病的AML患者的临床资料，包括治疗后完全缓解(CR)率、总生存(OS)时间、无病生存(DFS)时间以及近期和远期的不良反应。 结果 116例患者中，DA方案诱导治疗1个疗程后CR 78例(67.2%)，≤2个疗程CR共94例(81.0%)。 第1疗程诱导治疗中仅3例(2.6%)发生早期死亡。 有1例患者在诱导化疗后6个月时发现无症状性心脏射血分数减低。 89例患者接受了1~4个疗程巩固治疗。 在中位随访24(1~46)个月后，中位DFS时间为25个月，中位OS时间未到达。 Cox多因素分析显示，遗传学危险分层是影响DFS的独立预后因素(HR=0.258, 95% CI 0.100~0.664，P=0.005)，遗传学危险分层(HR=0.309, 95% CI 0.126~0.756，P=0.010)以及是否接受大于1个疗程大剂量阿糖胞苷巩固治疗(HR=0.370, 95% CI 0.179~0.765，P=0.007)是影响OS的独立预后因素。 结论 含DNR 60 mg·m−2·d−1的DA方案诱导治疗≤65岁初治AML患者可获得较高CR率，且不良反应无明显增加。
Methylmercury biogeochemistry: a review with special reference to Arctic aquatic ecosystems

There has been increasing concern about mercury (Hg) levels in marine and freshwater organisms in the Arctic, due to the importance of traditional country foods such as fish and marine mammals to the diet of Northern Peoples. Due to its toxicity and ability to bioaccumulate and biomagnify in food webs, methylmercury (MeHg) is the form of Hg that is of greatest concern. The main sources of MeHg to Arctic aquatic ecosystems, the processes responsible for MeHg formation and degradation in the environment, MeHg bioaccumulation in Arctic biota and the human health implications for Northern Peoples are reviewed here. In Arctic marine ecosystems, Hg(II) methylation in the water column, rather than bottom sediments, is the primary source of MeHg, although a more quantitative understanding of the role of dimethylmercury (DMHg) as a MeHg source is needed. Because MeHg production in marine waters is limited by the availability of Hg(II), predicted increases in Hg(II) concentrations in oceans are likely to result in higher MeHg concentrations and increased exposure to Hg in humans and wildlife. In Arctic freshwaters, MeHg concentrations are a function of two antagonistic processes, net Hg(II) methylation in bottom sediments of ponds and lakes and MeHg photodemethylation in the water column. Hg(II) methylation is controlled by microbial activity and Hg(II) bioavailability, which in turn depend on interacting environ- mental factors (temperature, redox conditions, organic carbon, and sulfate) that induce nonlinear responses in MeHg production. Methylmercury bioaccumulation-biomagnification in Arctic aquatic food webs is a function of the MeHg reservoir in abiotic com- partments, as well as ecological considerations such as food-chain length, growth rates, life-history characteristics, feeding behavior, and trophic interactions. Methylmercury concentrations in Arctic biota have increased significantly since the onset of the industrial age, and in some populations of fish, seabirds, and marine mammals toxicological thresholds are being exceeded. Due to the complex connection between Hg exposure and human health in Northern Peoples—arising from the dual role of country foods as both a potential Hg source and a nutritious, affordable food source with many physical and social health benefits—-reductions in anthro- pogenic Hg emissions are seen as the only viable long-term solution.
实时定量PCR检测BCR-ABL (P210）转录本水平室内长期质控体系的建立及应用

目的 制备BCR-ABL (P210）实时定量PCR（RQ-PCR）检测的室内质控物，建立RQ-PCR检测BCR-ABL (P210）转录本水平的室内质控方法。 方法 取K562细胞和HL-60细胞，制备RQ-PCR检测BCR-ABL（P210）转录本水平的高、低浓度质控物，2013年8月至2015年10月用RQ-PCR法与临床标本同步检测BCR-ABL（P210）184次，按试剂批号（批号20130303、20131212、20140411和20150327分别简称为R1、R2、R3、R4）统计分析标准曲线斜率、截距及相关系数，并按试剂批号和质控物批号（批号20130725、20140611分别简称为Q1、Q2）统计分析高、低浓度质控物检测结果，对斜率、截距及质控物检测结果采取Levey-Jennings质控图结合Westgard多规则质控方法进行质控判断。 结果 ①标准曲线斜率、截距：R1检测53次，斜率、截距均未失控；R2检测37次，斜率失控6次，且第2~8次在x−s限值下侧，第12~37次在x值上侧，截距失控9次，且第1~8次在x+s限值上侧，第12~37次出现在x值下侧；R3检测80次和R4检测14次，斜率、截距均未失控。 ②质控物结果：Q1批号质控物，R1检测49次未失控，R2检测23次失控1次；Q2批号质控物，R2检测14次、R3检测72次及R4检测14次均未失控。 结论 利用K562细胞和HL-60细胞制备定量检测BCR-ABL（P210）转录本水平的高、低浓度室内质控物，制备方便，检测结果可靠、稳定，使用质控物检测结果结合标准曲线斜率、截距进行室内质控，可更有效地保证临床检测结果的准确性和稳定性。
CD19 CAR-T细胞培养过程中PD-1蛋白、mRNA水平及细胞杀伤活性变化

目的 探讨CD19 CAR-T细胞培养过程中其PD-1蛋白、mRNA水平及细胞杀伤活性变化。 方法 收集6例外周血PD-1高表达恶性淋巴瘤患者、6例健康志愿者的外周血T细胞，作为CAR-T培养的T细胞来源。 流式细胞术检测PD-1蛋白表达、PCR法检测PD-1 mRNA水平，CCK-8法检测细胞增殖，LDH法检测细胞杀伤活性。 结果 ①PD-1高表达患者T细胞来源CD19 CAR-T细胞，与志愿者T细胞来源者相比，转染率无差异（P>0.05）；②PD-1高表达T细胞来源CAR-T细胞与PD-1抑制剂联合与否，以及健康志愿者CAR-T之间，细胞增殖差异无统计学意义（P>0.05）；③PD-1高表达T细胞与CAR-T细胞对淋巴瘤细胞株杀伤活性，低于二者联合PD-1抑制剂及志愿者CAR-T细胞（P<0.001），而PD-1高表达T细胞来源CAR-T细胞联合PD-1抑制剂与健康志愿者CAR-T细胞间差异无统计学意义（P>0.05）；④各组细胞培养过程中PD-1表达均下降，差异无统计学意义（P>0.05），但各组细胞培养过程中，PD-1 mRNA的变化差异无统计学意义（P>0.05）；⑤PD-1高表达T细胞来源CAR-T收获后，与PD-1抑制剂共培养与否，其PD-1表达差异无统计学意义（P>0.05），但CAR-T与淋巴瘤细胞株接触后，其PD-1表达随培养时间延长而增高，加入PD-1抑制剂可拮抗该作用；各组间PD-1 mRNA的变化差异无统计学意义（P>0.05）。 结论 PD-1高表达T细胞来源CAR-T细胞与肿瘤细胞接触后，其PD-1表达随培养时间延长而增高；而包括PD-1抑制剂在内，不能改变其PD-1 mRNA的表达。
On Lunar Periodicity in Spawning of normally grown Falmouth Oysters (O. edulis) in 1925, with a comparison of the spawning capacity of normally grown and dumpy Oysters.

During 1925 dumpy oysters spawned in a high proportion on the Fal beds, and although not such heavy breeders as normal oysters, they are undoubtedly valuable in forming a stable spawning stock for replenishing the beds in the future. Nearly every normal and dumpy oyster above a size of 2 to 2\ inches produced at least one batch of spawn in 1925. Since dumps increase in proportion on the beds as over-fishing occurs, they form a natural breeding reserve for repopiilating the beds in favourable years, as there is no adequate reason to suppose that the larvae of dumps are unhealthy. Weekly samples of oysters examined closely during the summer of 1925 for the percentage of spawning females and ripe but unspawned females show