Miracle-dz/UniFAR-FTUs huggingface dataset:
Caracterización de diferentes productos de bollería industrial. II. Estudio de la micoflora

Five different bakery products were screened for xerophilic mycobiota. Both expired and non-expired products were studied and separated analysis were carried out for the inside and the surface of each product. Diluting plating technique was used for non-expired product, while direct plating was applied to expired ones. None of the packets of non-expired products exceeded the maximum contamination level allowed by the Spanish legislation for bakery products (5 . 10 2 cfu . g -1 ) being the most commonly isolated fungi Aspergillus niger, A. flavus and Penicillium corylophilum. A higher number of fungal genera was found in expired products and, in general, a higher fungal concentration was found on the surface than inside the products. Most commonly isolated fungi were A. niger, A. flavus, P. corylophilum, Eurotium herbariorum, E. rubrum and Cladosporium spp.
Ovulation of the first dominant follicle arising after day 21 post partum in suckling beef cows

The effects of body condition score (BCS) of 2•0 or 3•0 units at calving (low v. moderate), post-partum energy intake at 0•6 or 1•0 MJ metabolizable energy (ME) per day per kg M 0•75 (low v. high) and unrestricted or restricted (once daily) suckling on the ability of cows to ovulate were studied in a 2 × 2 × 2 factorial design with each treatment replicated eight times. Calf isolation and restricted suckling were imposed shortly after selection of the first dominant follicle (DF) to emerge after day 21 post partum. The episodic release of LH (sampled at 15-min intervals for 10 h) was determined 48 h before and 48 h after the day calf isolation and restricted suckling commenced. Additional blood samples were collected weekly for plasma insulin determination. The mean interval from calving to first ovulation was shorter for cows in moderate than low BCS at calving (47•8 v. 57•1 days, s.e.d. = 4•50, P 8 mIU per l). There were indications of interactions between suckling treatment and BCS (P < 0•08), and suckling treatment and plasma insulin concentration (P < 0•06), on LH pulse frequency, which suggested that calf restriction could alleviate the suppressive effects of under nutrition on episodic LH release. Amongst cows suckling once daily, the non-ovulating animals had fewer LH pulses prior to restricted suckling and smaller, slower growing DF, indicating an inability of the DF to respond to increased LH pulse frequency following calf restriction. Cows of moderate BCS, particularly those with moderate to high levels of plasma insulin (≥ 5 mIU per l), responded favourably to restricted suckling. In contrast, excessively thin cows with low plasma insulin concentrations (<5 mIU per l), that had most to gain from restricted suckling, responded poorly.
Influence of unilateral ovariectomy performed before ovulation on ovarian function, steroid hormone levels and development of porcine fetuses

Corpus luteum (CL) activity is closely linked with initiation and maintenance of pregnancy and with fetal development. The present study was aimed to analyze the impact of unilateral ovarian removal on ovarian function, steroid hormone level and fetal distribution and development. Unilateral ovariectomy (uni-OVX) was performed in gilts one day before ovulation (group SHORT, n = 24), 20 days before subsequent ovulation (group LONG, n = 23) or ovaries remained intact (group INTACT, n = 22). Gilts were inseminated by single fixed-time laparoscopic intrauterine insemination (LIUI) after hormonal estrus synchronization. Two days before the end of a 15 day long altrenogest feeding, a part of gilts (n = 23) were surgically fitted with a jugular vein catheter and blood samples were collected to determine of estradiol (E2) and progesterone (P4) concentrations. All animals were slaughtered on day 30 of gestation and ovarian features as well as the number, weight and distribution of fetuses recorded. Altogether, 48 gilts (70%) were pregnant and pregnancy rates did not differ between groups. Short term uni-OVX affected CL number compared to intact and long term OVX gilts (9.9 ± 0.8 vs. 20.6 ± 1.9 and 17.5 ± 0.8; P < 0.05) and the number of fetuses (8.7 ± 0.5 vs. 15.0 ± 1.0 and 14.4 ± 1.1; P<0.05), respectively. Weights of individual CL were not influenced by treatment. Fetuses were differently distributed in the uterine horns after uni-OVX. A higher (P<0.05) proportion of fetuses was present always in the horn which bore the ovary. In all groups, weights of fetuses from uterine horns with an active ovary was similar; however, fetuses of the OVX horn of the SHORT group were lighter (P < 0.05). Steroid hormone profile was typical for pregnant gilts, but differences were observed between groups. Both, the preovulatory E2 concentrations and the early luteal phase P4 levels were higher (P < 0.05) in gilts of the INTACT and LONG groups. In summary, compared to intact gilts, only long term uni-OVX could compensate ovarian development. Short term uni-OVX affects (1) the total number of CL and fetuses, (2) the distribution and weight of fetuses in the uterine horns and (3) steroid hormone levels. Therefore, the time window, but not the uni-OXV per se, which alters the local supply of progesterone, has an impact on fetal development and survival.
Efeito da idade das fêmeas e de fatores ambientais sobre a reprodução do predador Chrysoperla externa (Hagen, 1861) (Neuroptera: Chrysopidae) - DOI: 10.4025/actascibiolsci.v25i2.2014

The biotic and abiotic factors affect the reproductive potential of insects. Thus, the effects of female age and environmental factors on reproduction of Chrysoperla externa (Hagen, 1861) (Neuroptera: Chrysopidae) were studied. Adults of the predator were reared in "PVC" cages, closed in the upper portion with a thin mesh, organdie, placed on cotton plants at a greenhouse. The largest production of eggs by female (87.7 eggs) occurred with 49 days and the smallest (32.2 eggs) with 63 days of female age. The remating occurred after the exhaustion of spermatic reservations, starting on 28th day. The fecundity of C. externa females increased with elevation of the relative humidity of air. It was also verified a fast embryonic development with increase of temperature and female age. All these results supply subsidies for knowledge of the reproduction of C. externa in cotton system
Effect of ligation of mesenteric lymph duct on coagulation function in rats with severe heat stroke

Objective To investigate the influences of mesenteric lymph duct ligation (LDL) on the coagulation function in rats with severe heat stroke (SHS). Methods Forty male Wistar rats were randomly and equally divided into control, heat stroke sham (HSS), HSS-LDL, severe heat stroke (SHS) and SHS+LDL groups. Mesenteric lymph ducts were ligated in HSC-LDL and SHS-LDL groups before reproduction of SHS model. SHS rat models were reproduced in a prewarmed incubator. Peripheral blood was drawn to determine the parameters pertaining to blood coagulability including prothrombin time (PT), activated partial thromboplastin time (APTT), D-dimmer and platelet count (PLT), and lung and kidney histopathology was observed after heat stroke. Results Compared with those in control group, no obvious changes were observed in the coagulation indices in HSS and HSS-LDL group. While PT and APTT significantly prolonged, PLT remarkably decreased, D-dimmer markedly increased in SHS group (P<0.05). The coagulation indices presented a recovery trend to certain extent in SHS-LDL group. Histopathological examination of the kidney and lung showed severe hemorrhagic and congestive lesions in SHS group. Mesenteric lymph duct ligation alleviated the coagulation disorders and histopathological lesions. Conclusion The entrance of toxic agents through lymphatic passage may be the pathogenetic factor in producing coagulopathy, and ligation of the mesenteric lymph ducts might prevent the entrance of toxic materials and alleviate the injury to the blood coagulation property and internal organs. DOI: 10.11855/j.issn. 0577-7402.2016. 02.02
Characteristics of Functional Constipation among Kindergarten Students in Kecamatan Jatinangor, West Java, Indonesia

Background : Functional constipation can occur in children and affect their quality of life, but there is still lack of awareness and inability to provide proper management. Therefore, early detection is important. The aim of this study was to identify the prevalence of functional constipation among kindergarten students and its risks. Methods : A quantitative descriptive study was conducted from April to May 2017 on kindergarten students in Kecamatan Jatinangor, West Java, Indonesia by using questionnaires for their parents. In total, 149 parents joined the study. The questionnaire was made based on the Rome IV Criteria with additional questions for the student’s characteristics comprised of sex, prematurity, allergy, family history of constipation, dietary fiber consumption, and parents’ education level. Descriptive analyses were conducted. Results : The prevalence of functional constipation among the students was 8.7% with the most frequent complaints were retentive posturing (76.9%), hard stool consistency (38.5%), and history of obstructing toilet by stool (38.5%). Characteristics of children with functional constipation were prematurity (15.4%), history of allergy (15.4%), low dietary fiber intake (from fruits, 69.2%, vegetables 84.6%, seeds 100%), and non-university educated parents (father 76.9%, mother 84.6%). Conclusions : Constipation among kindergarten students in Jatinangor is 8.7%, while the most apparent characteristics in constipated children is low dietary fiber intake.
A study of Notch signalling in developmental angiogenesis

Blood vessel patterning during angiogenesis is a guided process. Endothelial tip cells located at the end of vascular sprouts are important for vessel guidance. One of the aims of my studies is to determine how endothelial tip cell formation is regulated. Previous studies have shown that the Notch ligand, Delta-like 4 (Dll4), is prominently expressed in tip cells, therefore suggesting a role of Notch signalling in its regulation. In the mouse, suppression of Notch signalling by pharmacological inhibition of γ-secretase or genetic deletion of one Dll4 allele resulted in excessive endothelial tip cell formation and a poorly-patterned, hyperdense vessel network. Induction of Notch signalling by Jagged1 peptide treatment led to a decrease in tip cell formation and a sparse vessel network. Thus, the Notch pathway negatively regulates tip cell formation to promote a non-sprouting endothelial stalk cell. The Notch-regulated ankyrin repeat protein, Nrarp, is a Notch target gene that is highly expressed in stalk cells and endothelial cells located at vessel branchpoints. Deletion of Nrarp in mouse and morpholino knockdown of nrarp-a and nrarp-b in zebrafish resulted in decreased endothelial cell proliferation and junctional instability, leading to ectopic vessel regression and the formation of a sparse vessel network. In endothelial cells, loss of Nrarp function increased Notch signalling but decreased Wnt signalling, thereby confirming its role as a negative and positive modulator of Notch and Wnt signalling, respectively. Mice null for Lymphoid enhancer factor 1 (Lef1) or deficient in endothelial Ctnnb1 also displayed increased vessel regression and decreased vessel density, suggesting a role of canonical Wnt/β-catenin signalling in maintaining vessel stability. In conclusion, my studies show that Notch signalling regulates endothelial tip cell formation and vessel stability to fine-tune vessel patterning. Furthermore, I demonstrate that Nrarp provides a molecular link to Wnt signalling to regulate vessel stability.
A Novel Work Flow for Health Survey Analysis: Results on the Yazd Health Study Data (YaHS)

Health surveys are one of the most prominent sources of information in health and medical sciences. Analysis of a health survey not only gives information about the inherent features of the population under study but also sheds light on the interactions between different health factors. Even though there has been a multitude of methodologies proposed for survey analysis, many of them are biased towards the previous hypotheses, the fact which may lead to spurious results by neglecting the whole structure of relations among the factors. Here, we proposed a novel work flow on survey analysis. By gathering the previously less-utilized graphical models on survey analysis, newly-proposed methods, and commonly-used tools of prediction and hypothesis testing our work flow provides the medical community with an end-to-end pipeline for comprehensive statistical analysis of health surveys. To demonstrate the functionality of our work flow, we validate it on the Yazd Health Study (YaHS) dataset, in which a questionnaire of 300 questions from 10000 participants associated with 40 laboratory measurements from a subset of 4010 individuals is acquired from the residents of Yazd Greater Area of Iran. Many of our findings are aligned with previous medical knowledge. Most of our new findings are surprisingly observed in the clinics, each of which is potential to raise a promising hypothesis.
INFORMATIQUE ET BIOLOGIE MOLÉCULAIRE : NAISSANCE D'UNE NOUVELLE DISCIPLINE

Au début des années 70, la biologie moléculaire avait commencé sa longue ascension vers la prééminence. La double hélice était connue depuis 20 ans et les séquences d’un certain nombre de protéines et d’acides nucléiques avaient déjà été déterminées. Le premier article sur la séquence d’un ARN de transfert avait présenté la célèbre structure en feuille de trèfle, une réussite brillante d’analyse de séquence (1). Margaret Dayhoff avait publié plusieurs volumes de son Atlas of Protein Sequence and Stucture. Ce fut le point de départ pour plusieurs d’entre nous, qui commençaient à travailler dans ce domaine, lequel sera connu, 25 années plus tard, sous le nom d’« application à la biologie des méthodes mathématiques d’analyse » ou « Bioinformatique » (2). Ces volumes contiennent les séquences protéiques et nucléiques connues à l’époque. M. Dayhoff y a également inclus des articles provocateurs sur des alignements de séquences marquants et la notion de familles de gènes. Cependant, l’évènement qui a fait exploser le domaine d’analyses mathématiques des séquences fut l’invention des techniques rapides de séquençage de l’ADN par Sanger à Cambridge et par Maxam-Gilbert à Harvard. Les méthodes informatiques deviennent alors critiques pour lire les séquences elles-mêmes et l’habituelle analyse « à l’oeil » de ces séquences, dont le volume s’accroissait sans cesse, devenait, à la fois, de plus en plus imprécise et de plus en plus irréaliste.
Morphometry and relations of the rectum to the various anatomical landmarks: A cadaveric study

Background: The present study aimed to calculate the length of the rectum, its anterior peritoneal reflection and to assess the relations of the rectum to the various anatomical landmarks in cadavers. Also, to observe any gender differences. Material and Methods: The present study was performed on 18 sagittal sections of the pelvis of adult human cadavers. The distances were measured from the anal verge to the midpoint of sacral promontory, anterior peritoneal reflection, S3 vertebra,the tip of the coccyx and the lower border of pubic bone.Results: The level of anterior peritoneal reflections was 7.70±1.15 cm and 7.59±1.69 cm respectively for males and females. There were no statistically significant gender differences. Conclusion: The average length of the rectum did not reveal any significant gender differences. The level of anterior peritoneal reflection for the rectum would aid as a landmark for surgeons operating at this site without exploring the peritoneal cavity.
Functional and structural analysis of androgen receptors for anti-cancer drug discovery

Summary Understanding the molecular mechanism(s) by which Nuclear Receptors (NRs) regulate developmental processes is an essential step toward the utilization of one or many of these mechanisms for anticancer drug research. This manuscript discusses the structures, functions and molecular mechanisms that regulate the interactions of the Androgen Receptors (AR) as a model for potential drug targeting in hormonally-driven solid tumors. ARs ‘nuclear function, transcriptional factors and their cytoplasmic interaction with growth factor-initiated signaling pathways and coregulatory proteins are reviewed. Potential mechanisms for AR antagonism, inhibition, and modulation, as well as newer strategies are discussed.
ENCORE: Software for Quantitative Ensemble Comparison

There is increasing evidence that protein dynamics and conformational changes can play an important role in modulating biological function. As a result, experimental and computational methods are being developed, often synergistically, to study the dynamical heterogeneity of a protein or other macromolecules in solution. Thus, methods such as molecular dynamics simulations or ensemble refinement approaches have provided conformational ensembles that can be used to understand protein function and biophysics. These developments have in turn created a need for algorithms and software that can be used to compare structural ensembles in the same way as the root-mean-square-deviation is often used to compare static structures. Although a few such approaches have been proposed, these can be difficult to implement efficiently, hindering a broader applications and further developments. Here, we present an easily accessible software toolkit, called ENCORE, which can be used to compare conformational ensembles generated either from simulations alone or synergistically with experiments. ENCORE implements three previously described methods for ensemble comparison, that each can be used to quantify the similarity between conformational ensembles by estimating the overlap between the probability distributions that underlie them. We demonstrate the kinds of insights that can be obtained by providing examples of three typical use-cases: comparing ensembles generated with different molecular force fields, assessing convergence in molecular simulations, and calculating differences and similarities in structural ensembles refined with various sources of experimental data. We also demonstrate efficient computational scaling for typical analyses, and robustness against both the size and sampling of the ensembles. ENCORE is freely available and extendable, integrates with the established MDAnalysis software package, reads ensemble data in many common formats, and can work with large trajectory files.
AB0854 Dysfunctional pain component in ankylosing spondylitis patients

Background As a result of chronic inflammation, repetitive activation of primary afferent fibres changes the functional state and activity of Central nervous pathways. On this patients when describing their complaints use neuropathic pain descriptors include numbness, burning, tingling, increased pain response to a stimulus nabulivou etc. Objectives The study of chronic pain syndrome in patients with ankylosing spondylitis (AS) to identify dysfunctional component of pain (DCP). Methods Was studied 150 patients as 127 men and 23 women. The average age of 35, and 52±10,55, mean disease duration of 7.19±of 6.31. All the patients were performed clinical and rheumatological examination (indices BASDAI, BASFI), the assessment of pain intensity on visual analogue scale (VAS), to identify DCP were investigated neurological status with the use of questionnaires neuropathic pain DN4 and PainDETECT, and identifying the emotional-affective disorders (HADS questionnaire). Results 22 patients (14.7%) on the DN4 questionnaire revealed 4 or more points, but lesions of the somatosensory nervous system in these patients was not detected, hence 14,7% of patients were identified DCP. When comparing patients with the presence of TCS group I (22 people) and no DCP group II (128) it has been found that statistically significantly in patients in group I had higher pain intensity on VAS (6,09±185 vs 4,55±2,06, p=0.001, respectively); active disease BASDAI index (of 7.05±vs 1,58 4,87±2,16, p=0.001, respectively); the expression of functional impairment index BASFI (6,46±2,24 vs 4,05±2,81, p=0.001); the indicators of the questionnaire HADS in group I, consistent with the presence of clinically significant anxiety and lack of it in group II (10,09±2,86 vs 6,17±3,35, p=0.0001). However, duration of disease distinguish authentic in the groups was not (9,41±6,89 6,81 vs ±6,13 p=0,07). Correlation analysis revealed a significant relationship between DCP and the intensity of the pain in VAS back, as well as with the severity of anxiety, disease activity, functional disorders (p=0.05) Conclusions The study identified 22 patients, the presence of neuropathic pain descriptors in combination with anxiety disorders, resulting in the conclusion that, along with nociceptive component of pain in 14.7% of cases detected by the DCT. So the pain of some AS patients should be considered as a multicomponent syndrome, providing a comprehensive approach with the use of antidepressants (TCA and SSRI’s). Disclosure of Interest None declared
Assessment of fish growth performance under limiting environment conditions aquaculture nutrition subprogram

1. Generate critically identified data for incorporation into factorial growth models that describe nutrient supply and nutrient retention in relation to temperature. 2. Determine the maintenance dietary protein and energy requirements for Atlantic salmon at normal to high temperatures. 3. Develop biochemical tools to understand the effect of temperature on the growth performance of fish. 4. Determine the optimum dietary protein to energy ratio for Atlantic salmon at normal to high temperatures. 5. Use biochemical tools to understand the effect of temperature on protein turnover and growth performance in barramundi. 6. Use biochemical tools to understand the effect of temperature on protein turnover and growth performance in Atlantic salmon.
PCR and FISH Detection Extends the Range of Pfiesteria piscicida in Estuarine Waters

PCR and fluorescent in situ hybridization probes were used to assay for the presence of the dinoflagellate Pfiesteria piscicida in 170 estuarine water samples collected from New York to nothem Florida. 20% of samples tested positive for the presence of P. piscicida, including sites where fish kills due to Pjiesteria have occurred and sites where there was no historical evidence of such events. The results extend the lmown range of P. piscicida northward to Long Island, New York. The results also suggest that P. piscicida is a common, and normally benign, inhabitant of estuarine waters of the eastern
Menin : un nouvel acteur dans le cycle cellulaire ?

La NEM1 est un syndrome cancereux affectant de nombreuses glandes endocrines, qui resulte d’une predisposition genetique liee a des mutations du gene MEN1. La Menin, proteine codee par MEN1 possede de nombreux partenaires qui interviennent dans la dynamique de la chromatine, la regulation de la transcription et de la stabilite du genome. Le besoin de mieux connaitre les fonctions biochimiques de la Menin m’a conduit a tenter de produire la proteine purifiee en grande quantite. Ce faisant, j’ai pu apporter des informations sur la structure de la Menin. Sur un plan fonctionnel, j’ai etudie l’interaction entre Menin et l’oncosuppresseur p53, et montre que certaines modifications post-traductionnelles de p53 determinantes pour son activite et sa stabilite en reponse a un stress genotoxique, n’affectent pas l’interaction, rendant possible que ce soient des modifications de la Menin qui y jouent un role determinant. J’ai ainsi montre que la Menin pouvait etre mono-ubiquitinee. La Menin interfere egalement avec un autre regulateur du cycle cellulaire, la proteine RB dont elle semble reguler le niveau de phosphorylation et peut-etre meme la quantite totale dans la cellule.