Miracle-dz/UniFAR-FTUs huggingface dataset:
Regulation of Rhcg, an ammonia transporter, by aldosterone in the kidney.

Rh C glycoprotein (Rhcg), an ammonia transporter, is a key molecule in urinary acid excretion and is expressed mainly in the intercalated cells (ICs) of the renal collecting duct. In the present study we investigated the role of aldosterone in the regulation of Rhcg expression. In in vivo experiments using C57BL/6J mice, Western blot analysis showed that continuous subcutaneous administration of aldosterone increased the expression of Rhcg in membrane fraction of the kidney. Supplementation of potassium inhibited the effect of aldosterone on the Rhcg. Next, mice were subjected to adrenalectomy with or without administration of aldosterone, and then ad libitum 0.14M NH4Cl containing water was given. NH4Cl load increased the expression of Rhcg in membrane fraction. Adrenalectomy decreased NH4Cl-induced Rhcg expression, which was restored by administration of aldosterone. Immunohistochemical studies revealed that NH4Cl load induced the localization of Rhcg at the apical membrane of ICs in the outer medullary collecting duct. Adrenalectomy decreased NH4Cl-induced membrane localization of Rhcg, which was restored by administration of aldosterone. For in vitro experiments, IN-IC cells, an immortalized cell line stably expressing Flag-tagged Rhcg (Rhcg-Flag), were used. Western blot analysis showed that aldosterone increased the expression of Rhcg-Flag in membrane fraction, while the increase in extracellular potassium level inhibited the effect of aldosterone. Both spironolactone and Gӧ6983, a PKC inhibitor, inhibited the expression of Rhcg-Flag in the membrane fraction. These results suggest that aldosterone regulates the membrane expression of Rhcg through the mineralocorticoid receptor and PKC pathways, which is modulated by extracellular potassium level.
Predictors of literacy in adulthood: Evidence from 33 countries

What makes a literate person? What leads to literacy gains and losses within and between individuals and countries? This paper provides new evidence that helps answer these questions. The present comparative analysis of literacy is based on large representative samples from the Survey of Adult Skills conducted in 33 countries, with 25–65 year old participants. We provide, for the first time, estimates of relative importance for a comprehensive set of experiential factors, motivations, incentives, parental influence, demands of workplace, and other predictors of influence. We sketch a configuration of factors that predicts an “ideal” reader, i.e., the optimal literacy performance. Moreover, we discover a pivotal role of the age effect in predicting variability between countries. Countries with the highest literacy scores are the ones where literacy decreases with age the most strongly. We discuss this finding against current accounts of aging effects, cohort effects and others. Finally, we provide methodological recommendations for experimental studies of aging in cognitive tasks like reading.
Application of deep learning to predict underestimation in ductal carcinoma in situ of the breast with ultrasound

Background To develop an ultrasound-based deep learning model to predict postoperative upgrading of pure ductal carcinoma in situ (DCIS) diagnosed by core needle biopsy (CNB) before surgery. Methods Of the 360 patients with DCIS diagnosed by CNB and identified retrospectively, 180 had lesions upstaged to ductal carcinoma in situ with microinvasion (DCISM) or invasive ductal carcinoma (IDC) postoperatively. Ultrasound images obtained from the hospital database were divided into a training set (n=240) and validation set (n=120), with a ratio of 2:1 in chronological order. Four deep learning models, based on the ResNet and VggNet structures, were established to classify the ultrasound images into postoperative upgrade and pure DCIS. We obtained the area under the receiver operating characteristic curve (AUROC), specificity, sensitivity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) to estimate the performance of the predictive models. The robustness of the models was evaluated by a 3-fold cross-validation. Results Clinical features were not significantly different between the training set and the test set (P value >0.05). The area under the receiver operating characteristic curve of our models ranged from 0.724 to 0.804. The sensitivity, specificity, and accuracy of the optimal model were 0.733, 0.750, and 0.742, respectively. The three-fold cross-validation results showed that the model was very robust. Conclusions The ultrasound-based deep learning prediction model is effective in predicting DCIS that will be upgraded postoperatively.
Imaging sebaceous gland using optical coherence tomography with deep learning assisted automatic identification

Imaging sebaceous glands and evaluating morphometric parameters are important for diagnosis and treatment of serum problems. In this article, we investigate the feasibility of high‐resolution optical coherence tomography (OCT) in combination with deep learning assisted automatic identification for these purposes. Specifically, with a spatial resolution of 2.3 μm × 6.2 μm (axial × lateral, in air), OCT is capable of clearly differentiating sebaceous gland from other skin structures and resolving the sebocyte layer. In order to achieve efficient and timely imaging analysis, a deep learning approach built upon ResNet18 is developed to automatically classify OCT images (with/without sebaceous gland), with a classification accuracy of 97.9%. Based on the result of automatic identification, we further demonstrate the possibility to measure gland size, sebocyte layer thickness and gland density.
Perceived Discrimination and Increased Odds of Unmet Medical Needs Among US Children

Our study examines the association between perceived discrimination due to race and unmet medical needs among a nationally representative sample of children in the United States. We used data from the 2016-2017 National Survey of Children's Health, a population-based cross-sectional survey of randomly selected parents or guardians in the United States. We compared results from the coarsened exact matching (CEM) method and survey-weighted logistic regression to assess the robustness of the results. Using self-reported measures from caregivers, we find that ∼2.7% of US children have experienced racial discrimination with prevalence varying significantly by race. While <1% of non-Hispanic whites have experienced some measure of racism, this increases to 8.8% among non-Hispanic blacks. Perceived discrimination was associated with significantly greater odds of unmet medical needs in the adjusted, survey-weighted multivariate-adjusted model (adjusted odds ratio [OR] = 2.4 and 95% confidence interval [CI] = 1.2, 4.9) as well as in the CEM-model estimate (OR = 2.8 and 95% CI = 1.8, 4.0). Children who have experienced perceived discrimination had higher odds of unmet medical needs. Awareness of discrimination among children may help inform future intervention development that addresses unmet medical needs during childhood.
Effectiveness of transdiagnostic seminars to support patients with common mental disorders: a multi-service practice research network study

Abstract Background: This was a multi-site evaluation of psycho-educational transdiagnostic seminars (TDS) as a pre-treatment intervention to enhance the effectiveness and utilisation of high-intensity cognitive behavioural therapy (CBT). Aims: To evaluate the effectiveness of TDS combined with high-intensity CBT (TDS+CBT) versus a matched sample receiving CBT only. Second, to determine the consistency of results across participating services which employed CBT+TDS. Finally, to determine the acceptability of TDS across patients with different psychological disorders. Method: 106 patients across three services voluntarily attended TDS while on a waiting list for CBT (TDS+CBT). Individual and pooled service pre–post treatment effect sizes were calculated using measures of depression, anxiety and functional impairment. Effectiveness and completion rates for TDS+CBT were compared with a propensity score matched sample from an archival dataset of cases who received high-intensity CBT only. Results: Pre–post treatment effect sizes for TDS+CBT were comparable to the matched sample. Recovery rates were greater for the group receiving TDS; however, this was not statistically significant. Greater improvements were observed during the waiting-list period for patients who had received TDS for depression (d = 0.49 compared with d = 0.07) and anxiety (d = 0.36 compared with d = 0.04). Conclusions: Overall, this new evidence found a trend for TDS improving symptoms while awaiting CBT across three separate IAPT services. The effectiveness of TDS now warrants further exploration through an appropriately sized randomised control trial.
Image measurements of os odontoideum in children

Background Conservative therapy is used for children with odontoid fracture; however, when the odontoid fracture is complicated by significant displacement and unstable, surgery is required. Anterior cervical hollow lag screw fixation has been successfully used in adult patients, but until now, there has not been any relevant image measurement research in children with os odontoideum. The aim of the present study was to identify the morphometric changes of normal os odontoideum in children of different ages and to discuss parameters for screw fixation. Methods Computed tomography (CT) scanning data of normal os odontoideum in 120 children of different ages were measured. The parameters were as follows: transverse and vertical diameters of cancellous bone and cortical bone in os odontoideum basilar part, angle and distance from simulation screw insertion point (anterior mid-point of C2 vertebral body) to os odontoideum anterior angle as well as posterior angle, the optimal insertion angle, and the optimal screw length. Results The basilar part of normal os odontoideum was roughly round, and vertical diameter was slightly larger than transverse diameter. All parameters measured in the present study increased with age. The safety screw insertion angle range was 16–36°, and the optimal insertion angle ranged from 19° to 22°. The safety screw path length ranges in the 3–5-, 6–9-, and 10–14-year groups were 8–14, 10–16, and 12–21 mm, respectively, and the optimal screw length ranges were 13–14, 15–16, and 19–20 mm, respectively. The height of the axis showed a growing dimension followed by the advancing age in all groups. In each group, the height of the axis of the male is greater than the female. Conclusions For children undergoing odontoid screw fixation for the treatment of type II odontoid fracture, it is important to select the appropriate screw diameter, length, and direction according to parameter changes of os odontoideum based on their age.
High spatial resolution spiral first‐pass myocardial perfusion imaging with whole‐heart coverage at 3 T

To develop and evaluate a high spatial resolution (1.25 × 1.25 mm2) spiral first‐pass myocardial perfusion imaging technique with whole‐heart coverage at 3T, to better assess transmural differences in perfusion between the endocardium and epicardium, to quantify the myocardial ischemic burden, and to improve the detection of obstructive coronary artery disease.
INFLUENCE OF AMMONIUM : NITRATE RATIO AND NITROGEN CONCENTRATION ON NITRIFICATION ACTIVITY IN SOILLESS POTTING MEDIA

Effects of medium, NH4 : N0 3, ratio, and N fertilizer rate on the development of NH 4 oxidation in soilless potting media were evaluated. In two separate experiments, NH 4 oxidizing activity increased to a maximum (4 to 6 weeks of cropping) and then dropped off sharply. Ammonium oxidation activity varied significantly among types of soilless potting media. Media fertilized with 1 NH 4-N: 3 N0 3-N had higher rates of NH 4 oxidation than media fertilized with ratios of either 1:1 or 3:1. Nitrogen fertilization at 15 m M gave consistently higher oxidation rates than fertilization at 30 mM. In general, media samples that had been cropped with plants had higher nitrifying activity than unplanted samples. Ammonium oxidation rate over all observations was significantly correlated with medium pH (r = 0.50). pH values above 6.8 were necessary but not completely sufficient for relatively high rates of NH 4 oxidation. Rates of oxidation were insignificant with pH values <5.6. Nitrification is defined as the oxidation of NH 4 to NO 3 and is generally mediated by the activities of two groups of che- moautotrophic bacteria (Alexander, 1977). One group, the NH4 oxidizers, initiates the process with the formation of NO 2, while another group, the NO2 oxidizers, completes the process by converting NO2 to NO3. The nitrification process is sensitive to pH, water, and temperature and has an absolute requirement for
Rimonabant potentiates the antifungal activity of amphotericin B by increasing cellular oxidative stress and cell membrane permeability.

Amphotericin B (AmB) is a very effective antifungal agent, and resistance in clinical isolates is rare. However, clinical treatment with AmB is often associated with severe side effects. Reducing the administration dose of AmB by combining it with other agents is a promising strategy to minimize this toxicity. In this study, we screened a small compound library and observed that the anti-obesity drug rimonabant exhibited synergistic antifungal action with AmB against Candida species and Cryptococcus neoformans. Moreover, the combination of AmB and rimonabant exhibited synergistic or additive effects against C. albicans biofilm formation and cell viability in preformed biofilms. The effects of this combination were further confirmed in vivo using a murine systemic infection model. Exploration of the mechanism of synergy revealed that rimonabant enhances the fungicidal activity of AmB by increasing cellular oxidative stress and cell membrane permeability. These findings provide a foundation for the possible development of AmB-rimonabant polytherapies for fungal infections.
Use of an electromagnetic technique to determine sodicity in saline‐sodic soils

Soil sodicity is an increasing problem in arid‐land irrigated soils that decreases soil permeability and crop production and increases soil erosion. The first step towards the control of sodic soils is the accurate diagnosis of the severity and spatial extent of the problem. Rapid identification and large‐scale mapping of sodium‐affected land will help to improve sodicity management. We evaluated the effectiveness of electromagnetic induction (EM) measurements in identifying, characterizing and mapping the spatial variability of sodicity in five saline‐sodic agricultural fields in Navarre (Spain). Each field was sampled at three 30‐cm soil depth increments at 10–30 sites for a total of 267 soil samples. The number of Geonics‐EM38 measurements in each field varied between 161 and 558, for a total of 1258 ECa (apparent electrical conductivity) readings. Multiple linear regression models established for each field predicted the average profile ECe (electrical conductivity of the saturation extract) and SAR (sodium adsorption ratio of the saturation extract) from ECa. Despite the lack of a direct causal relationship between ECa and SAR, EM measurements can be satisfactorily used for characterizing the spatial distribution of soil sodicity if ECe and SAR are significantly auto‐correlated. These results provide ancillary support for using EM measurements to indirectly characterize the spatial distribution of saline‐sodic soils. More research is needed to elucidate the usefulness of EM measurements in identifying soil sodicity in a wider range of salt and/or sodium‐affected soils.
Vertebrate Evolution Conserves Hindbrain Circuits despite Diverse Feeding and Breathing Modes

Abstract Feeding and breathing are two functions vital to the survival of all vertebrate species. Throughout the evolution, vertebrates living in different environments have evolved drastically different modes of feeding and breathing through using diversified orofacial and pharyngeal (oropharyngeal) muscles. The oropharyngeal structures are controlled by hindbrain neural circuits. The developing hindbrain shares strikingly conserved organizations and gene expression patterns across vertebrates, thus begs the question of how a highly conserved hindbrain generates circuits subserving diverse feeding/breathing patterns. In this review, we summarize major modes of feeding and breathing and principles underlying their coordination in many vertebrate species. We provide a hypothesis for the existence of a common hindbrain circuit at the phylotypic embryonic stage controlling oropharyngeal movements that is shared across vertebrate species; and reconfiguration and repurposing of this conserved circuit give rise to more complex behaviors in adult higher vertebrates.
Sodium‐glucose co‐transporter‐2 inhibitors for the prevention of cardiorenal outcomes in type 2 diabetes: An updated meta‐analysis

A meta‐analysis of cardiorenal outcomes of sodium‐glucose co‐transporter‐2 inhibitors (SGLT‐2is) available in Europe or the United States in patients with type 2 diabetes (T2D) is presented. An electronic search up to 6 January 2021 was conducted to determine eligible trials. A total of eight cardiorenal outcomes trials of SGLT‐2is (empagliflozin, canagliflozin, dapagliflozin, ertugliflozin and sotagliflozin) were identified, with 65,587 patients. Data were analysed using a random effects model. Overall, SGLT‐2is were associated with a 12% reduced risk of major adverse cardiovascular events (MACE; HR = 0.88; 95% CI, 0.83–0.93; Q statistic, p = .19), with no significant heterogeneity (p for interaction = .465) between subgroups of patients with or without cardiovascular disease (CVD). The risk of the composite renal outcome was significantly reduced by treatment with SGLT‐2is (HR = 0.61, 95% CI, 0.54–0.70), with no significant heterogeneity of associations with outcome (I2 = 37%, p = .11), and no difference in the risk between patients with or without CVD (p for interaction = .665). SGLT‐2is have moderate benefits on MACE and major benefits on the progression of diabetic kidney disease.
Minimal incision bone graft and screw fixation for scaphoid nonunion with substantial bone loss

the lower extremities, although the true incidence remains unknown. As spontaneous resolution may occur, and relatively good results were reported using painkillers, such as gabapentin, we would still recommend conservative treatment as the first-line of treatment in these lesions. If surgery is undertaken, our case report suggests that unlike radical excision of infiltrating nerve tumours, for example neurofibromas or lipofibromatous hamartomas, neuritis ossificans can potentially be completely enucleated from the ulnar nerve without resulting in neurological deficits.
Barriers and facilitators to use of a mobile HIV care model to re-engage and retain out-of-care people living with HIV in Atlanta, Georgia

Novel strategies to re-engage and retain people living with HIV (PLWH) who are out of care are greatly needed. While mobile clinics have been used effectively for HIV testing and linkage, evidence guiding their use in providing HIV care domestically has been limited. To guide the development of a mobile HIV clinic (MHC) model as a strategy to re-engage and retain PLWH who are out of care, we aimed to explore stakeholder perceptions of barriers and facilitators to MHC implementation and use. From June 2019-July 2020, we conducted 41 in-depth interviews with HIV clinic providers, administrators, staff, legal authorities, and community advisory board members, PLWH, AIDS service organizations and city officials in Atlanta, Georgia, and domestic and international mobile health clinics to explore barriers and facilitators to use of MHCs. Interviews were transcribed, coded and thematically analysed. Barriers raised include potential for: breach of confidentiality with resulting heightened stigmatization, fractured continuity of care, safety concerns, staffing challenges, and low community acceptance of MHC presence in their locality. Participants provided suggestions regarding appropriate exterior design, location, timing, and co-delivery of non-HIV services that could facilitate MHC acceptance and address the concerns. In identifying key barriers and facilitators to MHC use, this study informs design and implementation of an MHC as a novel strategy for re-engaging and retaining PLWH who are out of care.
PLCδ1 Plays Central Roles in the Osmotic Activation of ΔN-TRPV1 Channels in Mouse Supraoptic Neurons and in Murine Osmoregulation

The magnocellular neurosecretory cells (MNCs) of the hypothalamus play a vital role in osmoregulation, but the mechanisms underlying MNC osmosensitivity are not fully understood. We showed previously that high osmolality activates phospholipase C (PLC) in rat MNCs in a Ca2+-dependent manner and that PLC activation is necessary for full osmotic activation of an N-terminal variant of the TRPV1 (ΔN-TRPV1) channel. We therefore hypothesized that the Ca2+-dependent δ1 isoform of PLC contributes to ΔN-TRPV1 activation and tested whether MNC function is defective in a transgenic PLCδ1 KO mouse. Water deprivation for 24 h caused greater increases in serum osmolality and losses in body weight in PLCδ1 KO mice than it did in control mice. Action potentials and ΔN-TRPV1 currents were measured in acutely isolated mouse MNCs using whole-cell patch clamp before and after exposure to hypertonic solutions. This treatment elicited a significant activation of ΔN-TRPV1 currents and an increase in firing rate in MNCs isolated from control mice, but not from PLCδ1 KO mice. Submembranous filamentous actin was measured in isolated MNCs before and after treatment with angiotensin II and hypertonic solution. Both treatments caused an increase in filamentous actin fluorescence in MNCs isolated from control mice, but both responses were significantly attenuated in MNCs from PLCδ1 KO mice. Our data demonstrate that the PLCδ1 isoform plays a key role in the activation of ΔN-TRPV1 channels and in osmosensory transduction in MNCs. This study advances our understanding of the molecular mechanisms underlying mammalian osmoregulation. SIGNIFICANCE STATEMENT Magnocellular neurosecretory cells (MNCs) of the hypothalamus play a central role in osmoregulation. We have identified a key role for the PLCδ1 isoform in the activation of ΔN-TRPV1 channels and osmosensory transduction in MNCs. The data indicate that the PLCδ1 isoform is activated by the Ca2+ influx occurring during MNC action potentials and exerts a positive feedback on ΔN-TRPV1 channels to enhance MNC excitability. This study provides evidence that PLCδ1 is a key molecule underlying osmosensory transduction, the regulation of VP release, and osmoregulation.