Miracle-dz/UniFAR-FTUs huggingface dataset:
Transcriptional Activation by a Matrix Associating Region-binding Protein

Bright (B cell regulator of IgH transcription) is a B cell-specific, matrix associating region-binding protein that transactivates gene expression from the IgH intronic enhancer (Eμ). We show here that Bright has multiple contextual requirements to function as a transcriptional activator. Bright cannot transactivate via out of context, concatenated binding sites. Transactivation is maximal on integrated substrates. Two of the three previously identified binding sites in Eμ are required for full Bright transactivation. The Bright DNA binding domain defined a new family, which includes SWI1, a component of the SWI·SNF complex shown to have high mobility group-like DNA binding characteristics. Similar to one group of high mobility group box proteins, Bright distorts Eμ binding site-containing DNA on binding, supporting the concept that it mediates Eμ remodeling. Transfection studies further implicate Bright in facilitating spatially separated promoter-enhancer interactions in both transient and stable assays. Finally, we show that overexpression of Bright leads to enhanced DNase I sensitivity of the endogenous Eμ matrix associating regions. These data further suggest that Bright may contribute to increased gene expression by remodeling the immunoglobulin locus during B cell development.
Auxiliary Circuits for Power Flow Control in Multifrequency Wireless Power Transfer Systems With Multiple Receivers

This paper describes a technique for multifrequency wireless power transfer systems in which the wireless power can be transmitted through the wireless power transfer channel or channels from the transmitter to the targeted loads with receiver coils specifically tuned for energy reception. Auxiliary circuits comprising bandpass and/or bandstop circuits are proposed for incorporation into the receiver circuits and optional relay circuits so as to facilitate the selection and enhancement of the wireless power transfer to the designated load without causing significant cross interference due to the use of multifrequency wireless power flow control. A unique feature of this technique is that the nontargeted receiver will automatically act as a relay resonator to enhance 1) magnetic coupling, and thus, 2) the power transfer between the power transmitter and the targeted receiver. A second novel feature is that the chosen operating frequencies for the tuned receivers need not be widely apart because the auxiliary circuits consist of bandpass and/or bandstop filters to reduce any cross interference from the nontargeted frequency. The proposed technique has been practically verified in experimental prototype.
A new coding scheme for packets in CDMA-based wireless ad hoc networks

We propose a novel packet coding scheme, transceiver packet coding scheme for DS-CDMA ad hoc networks. The design facilitates collision resolution by embedding the address information of both the transmitter and receiver in the coding scheme so that the receiver can identify the packet addressing to it without actually decoding the information symbol bits. In addition, the present scheme also avoids transmitter retransmissions and receivers wasting time in receiving a packet to completion if the packet is not addressed to it which causes additional battery energy consumption. A bank of matched filters is used to identify intended receivers, although any multiuser detection algorithm can easily be applied. The performance of the proposed coding scheme is studied by investigating its rate of missing receiver detection.
[The analysis of IL-10 and its methylation in the patients with acute on chronic liver failure].

OBJECTIVE To investigate the effect of IL-10 and the methylation of its promoter in acute on chronic liver failure (ACLF). METHODS Patients were divided into three groups: 25 with ACLF, 25 with CHB, 10 healthy controls. Respectively detect the serum level of IL-10 via ELISA, and the methylation of IL-10 promoter via MSP, to analyze the difference among the three groups. RESULTS Both the ACLF group and the CHB group have significant increase in serum level of IL-10 compared with the control group (P < 0.05); the ACLF group's level is higher than the CHB group, however without statistical significance (P > 0.05). The serum level of IL-10 in ACLF group has no significant relativity with ALT and HBV-DNA( r = -0.022, r = 0.033, respectively; P > 0.05); has positive relativity with TBIL and MELD ( r = 0.566, r = 0.443, respectively; P < 0.05); and negative relativity with PTA (r = -0.581, P < 0.05). The distribution of the methylation of IL-10 promoter in ACLF group is significantly different from the other two. CONCLUSION The serum level of IL-10 in hepatitis patients is significantly higher and increases with the degree of liver failure. The promoter methylation may be important in the gene inactivation.
Effects of a closed tracheal suction system on ventilatory and cardiovascular parameters.

OBJECTIVE To determine whether patients ventilated in the assist-control mode experienced a change in oxygenation, respiratory rate, inspiratory:expiratory ratio, heart rate, blood pressure or acid-base balance when suctioned with a closed tracheal suction system. DESIGN A quasi-experimental, within-subject, repeated-measures design was used. SUBJECTS 18 patients ventilated on a fraction of inspired oxygen of 0.47 +/- 0.17 and 2.3 +/- 5.0 cm H2O positive end-expiratory pressure. INTERVENTIONS Two suction passes were performed, with measurements at baseline, immediately after the first suction pass, immediately before the second suction pass, immediately after the second suction pass, 2 minutes after the second suction pass and 5 minutes after the second suction pass. No hyperoxygenation was used. RESULTS Significant differences were seen over time for arterial oxygen saturation, respiratory rate and inspiratory:expiratory ratio. Arterial oxygen saturation decreased to less than 90% in four subjects (range 88% to 89%), with a maximum fall of 9%. No significant differences were seen for heart rate, blood pressure, partial pressure of carbon dioxide, bicarbonate, time to nadir (lowest arterial oxygen saturation) or recovery time. CONCLUSIONS Subjects ventilated in the assist-control mode and suctioned with a closed tracheal suction system did not experience significant changes in cardiovascular or acid-base parameters when suctioned without hyperoxygenation. Although most subjects did not become desaturated, four subjects experienced desaturation at one or more intervals. To prevent desaturation, hyperoxygenation should be used before and after suctioning with a closed tracheal suction system.
Experiences With Governance in Three Marine Conservation Zoning Initiatives: Parameters for Assessment and Pathways Forward

In this perspective paper, we examine the challenges of governance in three marine conservation settings where rights, access to resources and zoning intersect with changing social and ecological conditions: (1) Tsitsikamma Marine Protected Area in South Africa; (2) Marine Protected Area of the Northern Coast of São Paulo (APAMLN) in Brazil; and (3) Gwaii Haanas National Marine Conservation Area Reserve in Canada. Many MPAs and related zoning initiatives are located adjacent to coastal communities that rely on marine and coastal resources for their livelihoods. Thus, processes of zoning must often address local use of natural resources which can be perceived by decision-makers and regulators as problematic. Our analysis highlights how conservation zoning intersects with the perception of diverse stakeholders regarding a range of governance dimensions, including: (1) levels of participation and compliance; (2) the clarity of zoning and conservation objectives; (3) livelihood impacts and benefits; (4) evidence of ecological and conservation benefits; and (5) the influence on sense of place. Pathways forward to address the challenges of governance associated with zoning include the importance of co-producing knowledge for more robust zoning outcomes, and situating zoning processes in a co-management context in which power and authority are more evenly distributed.
WALKING WITH COFFEE : WHY DOES IT SPILL ? PHYSICAL REVIEW E

In our busy lives, almost all of us have to walk with a cup of coffee. While often we spill the drink, this familiar phenomenon has never been explored systematically. Here we report on the results of an experimental study of the conditions under which coffee spills for various walking speeds and initial liquid levels in the cup. These observations are analyzed from the dynamical systems and fluid mechanics viewpoints as well as with the help of a model developed here. Particularities of the common cup sizes, the coffee properties, and the biomechanics of walking proved to be responsible for the spilling phenomenon. The studied problem represents an example of the interplay between the complex motion of a cup, due to the biomechanics of a walking individual, and the low-viscosity-liquid dynamics in it.
Jacques Delors, the Single Market and the Failed Attempt to Give a Soul to Europe

This working paper is concerned with the informal institutionalization of contacts between European institutions and religious organizations launched by Jacques Delors and its aftermath: the formal institutionalization of these contacts started by Jose Manuel Barroso. It was with Jacques Delors that the relationship between religion and the European project gained in importance. As president of the European Commission he recognised the social role of European religions by inviting them to the project “A Soul for Europe,” which aimed to find a more robust source of legitimacy for the European project. Subsequently, I describe the incorporation of Art. 17 in to the Lisbon Treaty obliging dialogue with churches, religious and philosophical organizations, which is in my opinion a direct aftermath of the “Soul for Europe. ” It is my argument in this paper that the “religious” project of Jacques Delors in many respects failed, as he did not find a meaningful role for religion in European integration in the way he was hoping for, nor did he find the robust source of legitimacy for the European project.
Microvascular Perfusion as a Target for Fluid Resuscitation in Experimental Circulatory Shock*

Objectives:To study regional perfusion during experimental endotoxemic and obstructive shock and compare the effect of initial cardiac output-targeted fluid resuscitation with optimal cardiac output-targeted resuscitation on different peripheral tissues. Design:Controlled experimental study. Setting:University-affiliated research laboratory. Subjects:Fourteen fasted anesthetized mechanically ventilated domestic pigs. Interventions:Domestic pigs were randomly assigned to the endotoxemic (n = 7) or obstructive shock (n = 7) model. Central and regional perfusion parameters were obtained at baseline, during greater than or equal to 50% reduction of cardiac output (T1), after initial resuscitation to baseline (T2), and after optimization of cardiac output (T3). Measurements and Main Results:Regional perfusion was assessed in the sublingual, intestinal, and muscle vascular beds at the different time points and included visualization of the microcirculation, measurement of tissue oxygenation, and indirect assessments of peripheral skin perfusion. Hypodynamic shock (T1) simultaneously decreased all regional perfusion variables in both models. In the obstructive model, these variables returned to baseline levels at T2 and remained in this range after T3, similar to cardiac output. In the endotoxemic model, however, the different regional perfusion variables were only normalized at T3 associated with the hyperdynamic state at this point. The magnitude of changes over time between the different vascular beds was similar in both models, but the endotoxemic model displayed greater heterogeneity between tissues. Conclusions:This study demonstrates that the relationship between the systemic and regional perfusion is dependent on the underlying cause of circulatory shock. Further research will have to demonstrate whether different microvascular perfusion variables can be used as additional resuscitation endpoints.
Example retrieval from a translation memory

Clustering of a translation memory is proposed to make the retrieval of similar translation examples from a translation memory more efficient, while a second contribution is a metric of text similarity which is based on both surface structure and content. Tests on the two proposed techniques are run on part of the CELEX database. The results reported indicate that the clustering of the translation memory results in a significant gain in the retrieval response time, while the deterioration in the retrieval accuracy can be considered to be negligible. The text similarity metric proposed is evaluated by a human expert and found to be compatible with the human perception of text similarity.
Serum autoantibodies as biomarkers for early cancer detection

Autoantibodies against autologus tumor‐associated antigens have been detected in the asymptomatic stage of cancer and can thus serve as biomarkers for early cancer diagnosis. Moreover, because autoantibodies are found in sera, they can be screened easily using a noninvasive approach. Consequently, many studies have been initiated to identify novel autoantibodies relevant to various cancer types. To facilitate autoantibody discovery, approaches that allow the simultaneous identification of multiple autoantibodies are preferred. Five such techniques – SEREX, phage display, protein microarray, SERPA and MAPPing – are discussed here. In the second part of this review, we discussed autoantibodies found in the five most common cancers (lung, breast, colorectal, stomach and liver). The discovery of panels of tumor‐associated antigens and autoantibody signatures with high sensitivity and specificity would aid in the development of diagnostics, prognostics and therapeutics for cancer patients.
Relationship of Mean Platelet Volume To MDD: A Retrospective Study

Background Results of numerous studies show that major depressive disorder (MDD) is associated with a chronic low-grade inflammation, but the underlying mechanism remains unclear. Aim To compare the results of blood cell analysis of MDD patients with healthy controls, and explore the potential value of it as an indicator of immune-inflammation in MDD, especially the mean platelet volume. Methods 103 MDD patients and 106 healthy controls with matched age and gender were recruited. We collected peripheral blood samples from both groups and gathered basic data. For comparison of normally distributed data (age, body mass index, lymphocyte count, white blood cell count, red blood cell count, hematocrit, platelet count and mean corpuscular volume) between groups, single t-test were used; and for comparison of non-normally distributed data (Neutrophil count, neutrophil count, platelet/ lymphocyte ratio, hemoglobin, red blood cell distribution width, mean platelet volume, mean hemoglobin concentration, mean hemoglobin and platelet distribution width), we used Mann-Whitney U-test. Results Compared with healthy controls, the MDD groups showed significantly higher white blood cell count (F=0.443, p=0.004), plateletcrit (F=8.3, p<0.001), neutrophil and lymphocyte ratio (Z=-6.063, p<0.001), neutrophil count (Z=-5.062, p<0.001), platelet/lymphocyte ratio (Z=-2.469, p=0.014), red blood cell distribution width (Z=-2.481, p=0.013) and mean platelet volume (Z=-2.668, p=0.008). In addition they had significantly lower hemoglobin (Z=-3.876, p<0.001), mean hemoglobin amount (Z=-3.005, p=0.003), red blood cell count (F=0.248, p<0.001), lymphocyte count (F=3.826, p=0.004) and hematocrit (F=0.000, p>0.001). Conclusions The results suggest that serum inflammatory response probably exists in people with MDD, and indicators of blood analysis especially mean platelet volume have a potential value as biomarker for inflammation.
Defining human endothelial progenitor cells

Summary. There is no specific marker to identify an endothelial progenitor cell (EPC) and this deficiency is restricting the ability of an entire field of research in defining these cells. We will review current methods to define EPC in the human system and suggest approaches to define better the cell populations involved in neoangiogenesis. PubMed was used to identify articles via the search term ‘endothelial progenitor cell’ and those articles focused on defining the term were evaluated. The only human cells expressing the characteristics of an EPC, as originally proposed, are endothelial colony forming cells. A variety of hematopoietic cells including stem and progenitors, participate in initiating and modulating neoangiogenesis. Future studies must focus on defining the specific hematopoietic subsets that are involved in activating, recruiting, and remodeling the vascular networks formed by the endothelial colony forming cells.
The influence of North American Aboriginal ethnicity on pro‐inflammatory and anti‐inflammatory cytokine responses to IFN‐alpha

Summary. North American Aboriginals have an enhanced propensity to clear HCV infection. Interferon (IFN)‐α is a critical agent in the clearance of hepatitis C virus (HCV) and other viruses; therefore the influence of Aboriginal ethnicity on IFN‐α responses was investigated in healthy Caucasian population control and Aboriginal cohorts. Cohort peripheral blood mononuclear cells produced similar levels of IFN‐α upon culture with reovirus, an innocuous virus capable of triggering IFN‐α synthesis. In addition, similar IFN‐γ synthesis was observed in the presence IFN‐α or reovirus. In contrast, Caucasian supernatants exhibited greater IL‐10 levels (P < 0.005), contributing to the overall cytokine balance as assessed by IFN‐γ/IL‐10 ratios being consistently elevated in the Aboriginal cohort. The potential of HCV proteins to alter IFN‐α cytokine induction was also investigated. Although there was some indication that HCV proteins might increase IFN‐α induced IL‐10 synthesis in Caucasians and conversely, IFN‐γ synthesis in Aboriginals, the addition of HCV proteins did not influence IFN‐γ/IL‐10 ratios. Finally, signal transducer and activator of transcription (STAT) 3 nuclear translocation was examined by western blot because it is a required intermediate in IFN‐α induced IL‐10 synthesis. Supporting the differential IL‐10 production, IFN‐α and core synergistically enhanced STAT3 nuclear translocation in Caucasian (P < 0.05); whereas, nuclear translocation of STAT3 remained unchanged in Aboriginal cells. Taken together, these findings suggest that ethnicity may influence certain responses to IFN‐α, possibly even in the presence of viral agents. These differences could impact early immune events allowing for enhanced viral clearance in Aboriginal populations.
SEMANTICAL AND ONTOLOGICAL CONSIDERATIONS IN TELOS: A LANGUAGE FOR KNOWLEDGE REPRESENTATION DIMITRIS PLEXOUSAKIS

This paper focuses on the semantics of Telos, a language for representing knowledge about information systems. Telos is intended to support the development of information systems, especially in the requirements modeling phase. An object‐oriented representational framework is supported by Telos. Its features include aggregation, generalization, and classification, the treatment of attributes as first‐class objects and the explicit representation of time. Telos also provides an assertion sublanguage for expressing deductive rules and integrity constraints. A possible‐worlds semantics is defined for Telos knowledge bases. This semantics is intended to capture the peculiarities involved in the interpretation of temporal expressions. The integration of time has also inspired the treatment of existence in Telos. An ontology of objects based on the property of existence is proposed. In the spirit of KRYPTON, Telos knowledge bases are specified functionally, in terms of the operations provided for querying and updating them. This knowledge‐level analysis will allow us to specify exactly what a knowledge base can be ASK‐ed or TELL‐ed about the domain of discourse. Soundness, consistency, and completeness results have also been proven to complete the specification of Telos knowledge bases. This formal account of the language provides a logical framework that can be used to verify the correctness of any proposed implementation of the system.
Prototipo de pulsera para la medida de ECG bajo demanda y la medida continua de la onda de pulso

El interes en la variabilidad de ritmo cardiaco (HRV) y la estimacion de la presion arterial a partir del tiempo de llegada de la onda de pulso (PAT) se ha incrementado exponencialmente en los ultimos anos debido a los indicadores que se pueden extraer. En este trabajo se presenta un prototipo de pulsera que, sin ningun otro medio externo a esta, es capaz de medir electrocardiograma (ECG), onda de pulso y, por consiguiente el PAT, de forma no invasiva. Ademas, esta disenada para la realizacion de medidas oportunistas y pensando en la comodidad del usuario. Se presentan tambien los materiales y electronica empleados para la implementacion del prototipo. A partir de los resultados obtenidos se da una guia para la colocacion de los sensores en la pulsera. Finalmente, los resultados indican que la calidad de las medidas realizadas es suficiente para ser utilizadas en el estudio de HRV y PAT.
CAROTENE PRODUCTION FROM AGRO-INDUSTRIAL WASTES BY Arthrobacter globiformis IN SHAKE-FLASK CULTURE

Industrial waste substrates, sugarcane molasses, and corn steep liquor were used for production of carotenes by Arthrobacter globiformis in this study. At the first stage, a one-factor-at-a-time approach was used for optimization of different media components such as carbon, nitrogen, MgSO4 · 7H2O, and KH2PO4, as well as pH, temperature, liquid medium volume, and inoculums level. The response surface method was further applied to determination of optimum values of process variables for maximum carotenes concentration. Results showed that the optimum combination for carotenes formation was as follows (g/L): sugarcane molasses, 40.00; corn steep liquor, 50.00; MgSO4 · 7H2O, 0.75; KH2PO4, 1.00. The maximum carotene concentration of 1.19 ± 0.02 mg/g dry biomass, about 113% of 1.05 ± 0.02 mg/g dry biomass growing in basal medium, was demonstrated by confirmatory experiments to be the optimum in liquid medium at 100 rpm, 30°C, initial pH of 7.5, and cultivation for 60 hr. In a second stage, detailed studies showed about 1.64-fold and 1.43-fold increase in carotene concentration (mg/g dry biomass) in the presence of addition of ethanol (4%, v/v) and addition of hydrogen peroxide (4%, v/v) at 40 hr, and 32 hr in liquid medium, separately.
EVOKED OCCIPITAL POTENTIALS RECORDED FROM SCALP ELECTRODES IN RESPONSE TO FOCAL VISUAL ILLUMINATION.

Evoked occipital potentials have been recorded, from scalp electrodes in response to focal visual illumination. To accomplish this, a computer specifically designed for this purpose and. capable of integrating any area under an evoked waveform and summing these areas to reveal a response has been used. A decoder is available which can signid when any preset digital value is reached or exceeded. Illumination of the optic papilla has been employed with different stimulus intensities and areas under conditions of light adaptation to demonstrate that focal retinal illumination can be achieved. Some patients with visual field defects have heen studied in an attempt to demonstrate the possibility of objective visual field testing.