Miracle-dz/UniFAR-FTUs huggingface dataset:
Layer-by-layer assembly of epidermal growth factors on polyurethane films for wound closure

To facilitate the healing of chronic wounds, growth factors such as epidermal growth factor need to be safely encapsulated for their sustained and effective delivery to the wound bed. Using a layer-by-layer assembly technique, epidermal growth factor is successfully encapsulated on the surface of poly(acrylic acid)-modified polyurethane film. The amount of encapsulated epidermal growth factor is controlled by adjusting the number of chitosan/epidermal growth factor bilayers. A controlled release of epidermal growth factor from the surface of polyurethane film for a period of five days is achieved with well-retained bioactivity (over 90%) as evidenced by a cell proliferation assay. In an in vitro cellular wounding assay, the cell gap covered with the epidermal growth factor-loaded polyurethane film closes at a rate more than twice as fast as that covered with a control polyurethane film. Fluorescent staining of F-actin reveals that the released epidermal growth factor induces differences in cytoskeletal organization, suggesting that stimulated cell migration also contributes to the close of the cell gap.
Experimental study on removal of NO using adsorption of activated carbon/reduction decomposition of microwave heating

Experimental studies were carried out on flue gas denitrification using activated carbon irradiated by microwave. The effects of microwave irradiation power (reaction temperature), the flow rate of flue gas, the concentration of NO and the flue gas coexisting compositions on the adsorption property of activated carbon and denitrification efficiency were investigated. The results show that: the higher of microwave power, the higher of denitrification efficiency; denitrification efficiency would be greater than 99% and adsorption capacity of NO is relatively stable after seven times regeneration if the microwave power is more than 420 W; adsorption capacity of NO in activated carbon bed is 33.24 mg/g when the space velocity reaches 980 per hour; adsorption capacity declines with increasing of the flow rate of flue gas; the change in denitrification efficiency is not obvious with increasing oxygen content in the flue gas; and the maximum adsorption capacity of NO was observed when moisture in flue gas was about 5.88%. However, the removal efficiency of NO reduces with increasing moisture, and adsorption capacity and removal efficiency of NO reduce with increasing of SO2 concentration in the flue gas.
Running head : AGE EFFECTS ON VISUOSPATIAL ASSOCIATIVE MEMORY 1 Memory for Important Item-Location Associations in Younger and Older Adults

Older adults typically experience memory impairments for verbal and visuospatial episodic information, which are most pronounced for associative information. While some age-related verbal memory deficits may be reduced by selectively focusing on high-value item information, the binding of items to locations in visuospatial memory involves different processes that are impaired in older adults. In the current study, we examined whether age-related impairment in visuospatial binding could be alleviated by strategic focus on important information and whether varying study time and presentation formats would affect such selectivity. We also used novel spatial resolutions analysis to examine participants’ gist-based visuospatial memory with respect to information importance. Younger and older adults were presented with items worth different point values in a visuospatial display, either sequentially (Experiment 1) or simultaneously (Experiment 2). When items were presented sequentially, participants became more selective with task experience, but when items were presented simultaneously, selectivity was maintained throughout the task. These patterns were also observed when encoding time was reduced for younger adults. Although older adults successfully engaged in value-based memory strategies, age-related visuospatial memory deficits were still present, even for high-value information, consistent with the associative deficit hypothesis. However, under some conditions, older adults showed reduced spatial relocation errors for high-value item-location associations. The results suggest that strategic control can be utilized when binding information in visuospatial memory, and that both younger and older adults can benefit by focusing on high-value items and their locations, despite associative memory deficits present in old age.
Tidal dissipation and evolution of white dwarfs around massive black holes: an eccentric path to tidal disruption

Awhite dwarf (WD)captured into a high-eccentricity orbit around amassive black hole (MBH) may undergo many pericentre passages before tidal disruption. During these passages, the tidal potential of the MBH excites internal oscillations or waves in the WD, and the dissipation of these oscillations can significantly influence the physical properties of the WD prior to its disruption. We calculate the amplitude of the tidally excited gravity (buoyancy) waves in the WD as a function of the pericentre distance and eccentricity for realistic WD models, under the assumption that these outgoing gravity waves are efficiently dissipated in the outer layers of the WD by non-linear effects or radiative damping. We obtain fitting formulae for the tidal energy and angular momentum transfer rates as well as the tidal heating rate.We find that these dynamical tides are much weaker than gravitational radiation in driving the orbital decay of theWD–MBH binary, and they are also inefficient in changing theWD spin during the orbital evolution. Incorporating our computed tidal dissipation rate into a MESA-based WD evolution code, we find that tidal heating can lead to appreciable brightening of theWD and may induce runaway fusion in the hydrogen envelope well before the WD undergoes tidal disruption.
Stress-related Transcription Factor AtfB Integrates Secondary Metabolism with Oxidative Stress Response in Aspergilli*

In filamentous fungi, several lines of experimental evidence indicate that secondary metabolism is triggered by oxidative stress; however, the functional and molecular mechanisms that mediate this association are unclear. The basic leucine zipper (bZIP) transcription factor AtfB, a member of the bZIP/CREB family, helps regulate conidial tolerance to oxidative stress. In this work, we investigated the role of AtfB in the connection between oxidative stress response and secondary metabolism in the filamentous fungus Aspergillus parasiticus. This well characterized model organism synthesizes the secondary metabolite and carcinogen aflatoxin. Chromatin immunoprecipitation with specific anti-AtfB demonstrated AtfB binding at promoters of seven genes in the aflatoxin gene cluster that carry CREs. Promoters lacking CREs did not show AtfB binding. The binding of AtfB to the promoters occurred under aflatoxin-inducing but not under aflatoxin-noninducing conditions and correlated with activation of transcription of the aflatoxin genes. Deletion of veA, a global regulator of secondary metabolism and development, nearly eliminated this binding. Electrophoretic mobility shift analysis demonstrated that AtfB binds to the nor-1 (an early aflatoxin gene) promoter at a composite regulatory element that consists of highly similar, adjacent CRE1 and AP-1-like binding sites. The five nucleotides immediately upstream from CRE1, AGCC(G/C), are highly conserved in five aflatoxin promoters that demonstrate AtfB binding. We propose that AtfB is a key player in the regulatory circuit that integrates secondary metabolism and cellular response to oxidative stress.
Fossil insect folivory tracks paleotemperature for six million years

Paleoecological studies enhance our understanding of biotic response to climate change because they consider timescales not accessible through laboratory or ecological studies. From 60 to 51 million years ago (Ma), global temperatures gradually warmed to the greatest sustained highs of the last 65 million years. Superimposed on this gradual warming is a transient spike of high temperature and pCO2 (partial pressure of carbon dioxide in the atmosphere; the Paleocene-Eocene Thermal Maximum 55.8 Ma) and a subsequent short-term cooling event (∼54 Ma). The highly resolved continental fossil record of the Bighorn Basin, Wyoming, USA, spans this interval and is therefore uniquely suited to examine the long-term effects of temperature change on the two dominant groups in terrestrial ecosystems, plants and insect herbivores. We sampled insect damage on fossil angiosperm leaves at nine well-dated localities that range in age from 52.7 to 59 Ma. A total of 9071 leaves belonging to 107 species were examined for the presence or absence of 71 insect-feeding damage types. Damage richness, frequency, and composition were analyzed on the bulk floras and individual host species. Overall, there was a strong positive correlation between changes in damage richness and changes in estimated temperature, a weak positive relationship for damage frequency and temperature, and no significant correlation for floral diversity. Thus, insect damage richness appears to be more sensitive to past climate change than to plant diversity, although plant diversity in our samples only ranges from 6 to 25 dicot species. The close tracking of the richness of herbivore damage, a presumed proxy for actual insect herbivore richness, to both warming and cooling over a finely divided, extended time interval has profound importance for interpreting the evolution of insects and plant–insect associations in the context of deep time. Our results also indicate that increased insect herbivory is likely to be a net long-term effect of anthropogenic warming.
Change in the Upper Airway of Patients With Obstructive Sleep Apnea Syndrome Using Computational Fluid Dynamics Analysis: Conventional Maxillomandibular Advancement Versus Modified Maxillomandibular Advancement With Anterior Segmental Setback Osteotomy

Objective:The aim of the study was to compare the effect of conventional maxillomandibular advancement (MMA) and modified MMA with anterior segmental setback osteotomy (MMA-ASSO) on the airway changes in patients with obstructive sleep apnea syndrome (OSAS) using three-dimensional computational fluid dynamics (3D-CFD) analysis. Methods:Two adult male patients with Class I malocclusion, lip protrusion, acute nasolabial angle, and OSAS were treated with conventional MMA (Case 1) and modified MMA-ASSO (Case 2). Individualized 3D airway models were fabricated using computed tomography data obtained 1 month before (T0) and at least 6 months after surgery (T1). A total of 7 cross-sectional areas of the airway were established, starting just above the hard palate (plane 1) with interval of 1 mm caudally. Airflow velocity and negative pressure were investigated using CFD analysis, and polysomnography studies were performed at T0 and T1. Results:There were improvement of apnea-hypoapnea index and the lowest O2 level (T0 versus T1; 43.2 versus 15.2, 79% versus 90% in Case 1; 61.0 versus 6, 89% versus 92% in Case 2). At plane 2 (retropalatal area) in Cases 1 and 2, there were increase in the smallest cross-sectional areas (57.9% versus 28.4%), decrease in the airflow velocity and increase in the negative pressure at the peak of expiration (49.5% versus 31.7%; 88.4% versus 54.3%), end after expiration (53.2% versus 32.2%; 83.2% versus 47.9%), and peak of inspiration (53.1% versus 29.2%; 75.3% versus 48.2%). Conclusion:Modified MMA-ASSO method might be an effective treatment option for OSAS patients with improvement of airway problems and esthetic facial profile.
A Super Wideband Optical FM Modulation Scheme for Video Transmission Systems

A novel super wideband optical FM modulation scheme that employs an optical heterodyne detection technique is proposed and demonstrated. The experimental results show that applying this scheme to 40-channel AM video transmission expands the power budget by approximately 10 dB at a carrier- to-noise ratio (CNR) of 42 dB. The feasibility of the scheme is also discussed from the viewpoints of connector reflectivity and fiber-dispersion.
A new species of Malaxis (Orchidaceae) from Guerrero, Mexico

Malaxis molotensis, a new orchid species from a wet cloud forest of the Sierra Madre del Sur, Guerrero, Mexico is described and illustrated. In habit and floral structure it resembles the widespread M. histionantha but differs in the elliptic labellum with incurved distal margin, and elongate, oblong-falciform pollinaria.
A novel fabric WLAN antenna for wearable applications

The development of wearable computer systems and smart clothing has been rapid. They are coming more and more lightweight and quite soon we will see a wide range of unobtrusive wearable and ubiquitous computing equipment integrated to into our everyday wear. Rapid progress in wireless communication promises to replace wired-communication networks in the near future in which antennas have a more important role. This paper presents a novel fabric WLAN antenna for wearable applications such as sports wear and emergency worker outfits. The substrate of the patch antenna is made of a very low-cost fleece fabric. The radiating element and groundplane are made of knitted copper. To our best knowledge this is the first compact fabric antenna design for commercial smart clothing. Measured results are compared with simulations and good agreement was observed.
Arabidopsis ECERIFERUM 2 Is a Component of the Fatty Acid Elongation Machinery Required for Fatty Acid Extension to Exceptional Lengths 1 [ W ] [ OA ]

Primary aerial surfaces of land plants are coated by a lipidic cuticle, which forms a barrier against transpirational water loss and protects the plant from diverse stresses. Four enzymes of a fatty acid elongase complex are required for the synthesis of verylong-chain fatty acid (VLCFA) precursors of cuticular waxes. Fatty acid elongase substrate specificity is determined by a condensing enzyme that catalyzes the first reaction carried out by the complex. In Arabidopsis (Arabidopsis thaliana), characterized condensing enzymes involved in wax synthesis can only elongate VLCFAs up to 28 carbons (C28) in length, despite the predominance of C29 to C31 monomers in Arabidopsis stem wax. This suggests additional proteins are required for elongation beyond C28. The wax-deficient mutant eceriferum2 (cer2) lacks waxes longer than C28, implying that CER2, a putative BAHD acyltransferase, is required for C28 elongation. Here, we characterize the cer2 mutant and demonstrate that green fluorescent protein-tagged CER2 localizes to the endoplasmic reticulum, the site of VLCFA biosynthesis. We use site-directed mutagenesis to show that the classification of CER2 as a BAHD acyltransferase based on sequence homology does not fit with CER2 catalytic activity. Finally, we provide evidence for the function of CER2 in C28 elongation by an assay in yeast (Saccharomyces cerevisiae).
A Modified Formulation of the Volume Integral Equations Method for 3-D Magnetostatics

A magnetization-based formulation of the volume integral equations method for 3-D magnetostatics is discussed. The magnetization of each element is related, via the constitutive law of the material, to the average magnetic flux density within the element rather than to the value at the center as is usually done. This assumption leads to more accurate distribution of magnetization and allows a faster convergence of the solution. Moreover, it leads to more symmetric matrix of the coefficients and reduces the numerical instability due to looping patterns of magnetization, which is inherent to integral methods. The formulation is made effective by the use of a hybrid numerical and analytical approach, which allows for the fast and accurate calculation of the coefficients. The proposed model is validated and compared with the usual model both for saturable and linear material with high susceptibility.
Interannual memory effects for spring NDVI in semi‐arid South Africa

Almost 20 years of Normalized Difference Vegetative Index (NDVI) and precipitation (PPT) data are analysed to better understand the interannual memory effects on vegetation dynamics observed at regional scales in Southern Africa (SA). The study focuses on a semi‐arid region (25°S–31°S; 21°E–26°E) during the austral early summer (September–December). The memory effects are examined using simple statistical approaches (linear correlations and regressions) which require the definition of an early summer vegetation predictand (December NDVI minus September NDVI) and a consistent set of potential predictors (rainfall amount, number of rainy days, rainfall intensity, NDVI and Rain‐Use‐Efficiency) considered with 4 to 15‐month time‐lag. An analysis over six SA sub‐regions, corresponding to the six major land‐cover types of the area reveals two distinct memory effects. A “negative” memory effect (with both rainfall and vegetation) is detected at 7 to 10‐month time‐lag while a “positive” memory effect (with vegetation only) is observed at 12 to 14‐month time‐lag. These results suggest that interannual memory effects in early summer vegetation dynamics of semi‐arid South Africa may preferably be driven by biological rather than hydrological mechanisms.
Structural insights into biased G protein-coupled receptor signaling revealed by fluorescence spectroscopy

G protein-coupled receptors (GPCRs) are seven-transmembrane proteins that mediate most cellular responses to hormones and neurotransmitters, representing the largest group of therapeutic targets. Recent studies show that some GPCRs signal through both G protein and arrestin pathways in a ligand-specific manner. Ligands that direct signaling through a specific pathway are known as biased ligands. The arginine-vasopressin type 2 receptor (V2R), a prototypical peptide-activated GPCR, is an ideal model system to investigate the structural basis of biased signaling. Although the native hormone arginine-vasopressin leads to activation of both the stimulatory G protein (Gs) for the adenylyl cyclase and arrestin pathways, synthetic ligands exhibit highly biased signaling through either Gs alone or arrestin alone. We used purified V2R stabilized in neutral amphipols and developed fluorescence-based assays to investigate the structural basis of biased signaling for the V2R. Our studies demonstrate that the Gs-biased agonist stabilizes a conformation that is distinct from that stabilized by the arrestin-biased agonists. This study provides unique insights into the structural mechanisms of GPCR activation by biased ligands that may be relevant to the design of pathway-biased drugs.
The protective effect of intranasal immunization with inactivated influenza virus vaccine

Protection against influenza after killed influenza spray vaccine was assessed in nine double blind, placebocontrolled clinical trials and two field studies, involving a total number of 10,000 subjects. Monovalent vaccine, containing 300-360 IU A/Hong Kong/1/68 (H3N2) or bivalent vaccine, containing 300-360 IU A/Aichi/2/68 (H3N2) and 200-240 IU B/Mass/3/66 per dose was used. Administration by the intranasal route appeared to be acceptable as an alternative to the parenteral route. The protective efficacy found was comparable with that attained after parenteral vaccination. It has, however, the advantages of a characteristic lack of side-effects and of the fact that no injection is involved. This will probably enhance the acceptance of influenza immunization by the population and thus lead to a higher vaccination rate in the communities we want to protect against influenza.
Development of Light‐activated Pupilloconstriction in Rats as Mediated by Normal and Transplanted Retinae

The relationship between the development of the pupilloconstriction response to changes in light levels and retinal maturation was studied in normal rats and rats that had received intracranial retinal transplants at birth. A pupillary response to light was first observed between postnatal days 7 and 9 in normal rats, and was typically of small amplitude and sluggish. By the time the eyelids first open, 2 weeks after birth, the pupillary response had improved to near adult levels. The inception of the pupillary response correlates with the first appearance of conventional synaptic contacts in the inner and outer plexiform layers of the retina, while improved responses correlate with maturation of photoreceptor outer segments and formation of synaptic ribbons in the inner plexiform layer. When embryonic retinae were transplanted to intracranial locations in newborn hosts and the transplants later illuminated as the host matured, the onset of a pupillary response to transplant illumination was delayed in proportion to the developmental disparity between the transplant and the host. The pattern of anatomical development in transplanted retinae was also similar, but delayed in time, compared to normal retinae. This indicates that the limiting factors for expression of light‐activated pupilloconstriction exist within the retina, rather than being intrinsic to the central nuclei or to the output pathway subserving the response.
Tissue-Engineered Bone Using Mesenchymal Stem Cells and a Biodegradable Scaffold

Bone marrow has been shown to contain a population of rare cells capable of differentiating to the cells that form various tissues. These cells, referred to as mesenchymal stem cells (MSCs), are capable of forming bone when implanted ectopically in an appropriate scaffold. The aim of this study was to investigate the potential of a new &bgr;-tricalcium phosphate (&bgr;-TCP) as a scaffold and to compare the osteogenic potential between &bgr;-TCP and hydroxyapatite (HA). The &bgr;-TCP and HA loaded with MSCs were implanted in subcutaneous sites and harvested at 1, 2, 4, and 8 weeks after implantation for biochemical and histological analysis. Biochemically, in both &bgr;-TCP and HA composites, the alkaline phosphatase activity in the composites could be detected and was maintained at a high level for 8 weeks. In the histological analysis, active bone formation could be found in both the &bgr;-TCP and HA composites. These findings suggest that &bgr;-TCP could play a role as a scaffold as well as HA. The fabricated synthetic bone using biodegradable &bgr;-TCP as a scaffold in vivo is useful for reconstructing bone, because the scaffold material is absorbed several months after implantation.
Formalising the Bitcoin protocol Making it a bit better

Bitcoin is a new, popular currency which is based on mathematical and cryptographic principles. The system relies on a decentralized peer-to-peer network of participants in which a majority decides on the validity of transactions. In this paper, the communication protocol used by Bitcoin to communicate between participants is investigated. We formally describe the protocol by specifying it in mCRL2. Using scenario-based verication, we verify that the Bitcoin protocol satises a number of requirements under various scenarios.
Trastuzumab: Further Considerations

One of the best examples of the "bench to bedside" process is the development of trastuzumab (Herceptin) for HER2-overexpressed breast tumors. From the identification of the neu oncogene in 1984[1] and its subsequent cloning,[2] to the development of a humanized monoclonal antibody targeting HER2 that improved outcome not only in the metastatic setting[3] but also in the adjuvant setting[4-7] has been a long yet fruitful journey.