Miracle-dz/UniFAR-FTUs huggingface dataset:
Laminin-10 mediates basal and EGF-stimulated motility of human colon carcinoma cells via alpha(3)beta(1) and alpha(6)beta(4) integrins.

Signals from the epidermal growth factor (EGF) receptor and integrin-dependent adhesion to laminin contribute to the progression and metastasis of colonic tumors. However, little is know about the mechanisms by which these signals cooperate. Recently, we have reported that the colon cancer cell line LIM1215 secretes and adhere to autocrine laminin-10 via multiple integrin receptors and that EGF stimulates spreading of these cells on the same substrate. In this report, we investigate the effect of EGF and laminin-10 on colon cancer cell migration in vitro. EGF stimulates migration of LIM1215 cells in a wound healing assay. The response to EGF is inhibited by anti-EGF receptor antibody 528, the EGF receptor kinase inhibitor AG-1478, or the MAP kinase kinase inhibitor PD98059 but not the PI3-K inhibitor wortmannin. Using Transwell migration chambers, we demonstrate that laminin-10 but not collagen-I, collagen-IV, or a commercial preparation of human placental laminin is a potent motility factor for LIM1215 cells. The migration response to laminin-10 is increased upon stimulation of the cells with EGF and correlates with the up-regulation of alpha(6)beta(4) integrin expression as measured by analysis of Triton X-100-soluble cellular extracts. The results from integrin inhibition experiments indicate that basal migration on laminin-10 is mediated by alpha(3)beta(1) but not alpha(2)beta(1) nor alpha(6)beta(4) integrins. Alpha(3) blocking antibodies also inhibited EGF-stimulated chemokinetic migration of LIM1215 cells on laminin-10. However, in contrast to unstimulated cells, alpha(6) or beta(4) integrin-blocking antibodies inhibited the migration of EGF-stimulated cells by up to 50%. Taken together, these results support the cooperative role of EGF receptor and laminin-10 on colon cancer cell motility and suggest a critical role for both the alpha(3)beta(1) and the alpha(6)beta(4) integrins in this process.
A comprehensive review of endoscopic ultrasound core biopsy needles

ABSTRACT Introduction: Endoscopic ultrasound (EUS)-guided tissue acquisition by-fine needle biopsy (EUS-FNB) developed over the last two decades as an attempt to overcome the limitations of fine needle aspiration (FNA). There are now three commercially available second-generation FNB needles with different tip designs. Areas covered: In this review the roles of EUS-FNA and FNB, the history and evolution of the EUS core biopsy needle are addressed followed by a presentation of currently available needles. Literature search was conducted using MEDLINE, Controlled Trials Register, US Patent Registry, Google Scholar, and Conference Abstracts. Expert commentary: While FNA remains the reference standard, it is limited by the inability to retain stroma and associated cellular architecture in biopsy samples. Histologic architecture is of paramount importance in providing a molecular diagnosis and for accurate tumor staging. FNB offers a superior diagnostic yield to FNA and initial experiences with the three commercially available second-generation FNB needles show highly promising results.
Glial Fibrillary Acidic Protein–Apolipoprotein E (apoE) Transgenic Mice: Astrocyte-Specific Expression and Differing Biological Effects of Astrocyte-Secreted apoE3 and apoE4 Lipoproteins

The ε4 allele of apolipoprotein E (apoE) is associated with increased risk for Alzheimer’s disease (AD) and poor outcome after brain injury. In the CNS, apoE is expressed by glia, predominantly astrocytes. To define the potential biological functions of different human apoE isoforms produced within the brain, transgenic mice were generated in which human apoE3 and apoE4 expression is under control of the astrocyte-specific glial fibrillary acidic protein (GFAP) promoter. These animals were then bred back to apoE knock-out mice. Human apoE protein is found within astrocytes and the neuropil throughout development and into the adult period, as assessed by immunocytochemistry and immunoblot analysis in several GFAP-apoE3 and E4 lines. Cultured astrocytes from these mice secrete apoE3 and apoE4 in lipoproteins that are high-density lipoprotein-like in size. When primary hippocampal neurons are grown in the presence of astrocyte monolayers derived from these transgenic mice, there is significantly greater neurite outgrowth from neurons grown in the presence of apoE3-secreting astrocytes compared with apoE4-secreting or apoE knock-out astrocytes. These effects are not dependent on direct astrocyte–neuron contact and appear to require the low-density lipoprotein receptor-related protein. These data suggest that astrocyte-secreted, apoE3-containing lipoproteins have different biological effects than apoE4-containing lipoproteins. In addition to providing information regarding the role of astrocyte-secreted apoE lipoproteins in the normal brain, these animals will also be useful in models of both AD and CNS injury.
Research on multi-Agent cooperation mechanism based on game theory

Aiming at the cooperation of Agent in multi-Agent system, the game theory was introduced to the cooperation of Agent, and the multi-Agent cooperation mechanism based on game theory was established. The common multi-Agent cooperation model was analyzed, and an improved benevolent function was given, as well as the specific process of cooperation strategies and cooperation algorithms. At last, the cooperation mechanism of multi-Agent system was tested through the pursuit-evasion problem. The results of simulated experiments indicated that the multi-Agent cooperation mechanism based on game theory can be effectively used to cooperation of multi-Agent.
Studies of bystander effects in human fibroblasts using a charged particle microbeam.

PURPOSE To determine the role of cell-to-cell mediated effects in the response of cells to different radiations by using a charged particle microbeam capable of targeting individual cells with counted particles. MATERIALS AND METHODS Primary human fibroblasts were irradiated conventionally with X-rays or alpha-particles and scored for the induction of micronucleated or apoptotic cell formation at various times after irradiation. Cells were also individually irradiated with helium-3 particles from a microbeam and the distribution of damaged cells between hit and non-hit cells scored. RESULTS Conventionally exposed fibroblasts showed a dose-dependent production of micronucleated and apoptotic cells 3 days after irradiation for both X-rays and alpha-particles, with a high RBE value for alpha-particles at low doses. Targeting individual alpha-particles, using a microbeam, to four cells within a population produced more micronucleated and apoptotic cells than expected on the basis of a direct effect only. CONCLUSIONS This study provides direct evidence for the production of transmissible, cell-to-cell effects between hit and non-hit cells individually exposed to charged particles.
A identificação de novas descobertas científicas através da análise do conhecimento contido em artigos científicos

A comunicacao cientifica e um componente fundamental da Ciencia institucionalizada, pois atraves dessa atividade que novos conhecimentos sao comunicados aos pares e a validade e consistencia dos mesmos e verificada. A identificacao de novos conhecimentos cientificos se da atualmente atraves de um longo processo social que depende da producao de textos, sua aceitacao num periodico cientifico, sua publicacao, leitura, critica e citacao pelos pares, ate que o novo conhecimento, de uma forma difusa, seja considerado valido e finalmente incorporado ao que Ziman chama de “conhecimento publico”. Este “conhecimento publico” se encontra hoje sob a forma principalmente das colecoes de periodicos distribuidas pelas bibliotecas. A Ciencia da Informacao se utiliza das publicacoes cientificas para, com base em tecnicas bibliometricas e cientometricas, identificar tendencias de desenvolvimento da Ciencia. As tecnologias trazidas com a Web Semântica trazem o potencial de alterar esta situacao. A Web Semântica traz a possibilidade da publicacao de documentos cientificos cujo conteudo que possam ser “compreendido” por programas e tambem a possibilidade de que o conhecimento numa determinada area possa ser formalizada, registrado e compartilhado nas chamadas ontologias, bases de conhecimento legiveis por programas e disponibilizadas na Web. Nos anos recentes este processo de registrar o conhecimento de dominios cientificos especificos sob a forma de ontologias publicas na Web vem se intensificando. Ontologias especificas na area de Ciencias da Saude que vem sendo largamente usadas para registro, sistematizacao, padronizacao de terminologia e compartilhamento de conhecimentos sao o UMLS e a GeneOntology. Esta pesquisa discute a possibilidade de, uma vez que publicacoes cientificas e ontologias possam ter o conhecimento neles contido representado de forma uniforme, em formato legivel por programas, programas agentes de “software” possam ser usados para comparar o conhecimento contido em artigos publicados na Web com o conhecimento contido em ontologias disponiveis na Web, permitindo assim identificar indicios de novas descobertas. A partir da apresentacao de um modelo ja desenvolvido com a finalidade de identificar e registrar o conhecimento contido no texto de artigos cientificos, as bases teoricas e conceituais do modelo sao apresentadas e discutidas as possibilidades e viabilidade de seu desenvolvimento para permitir a identificacao de novidades em Ciencia.
ATL

Objective This study aimed to investigate the status of DNA methylation of 6 genes, LMX1A, NKX6-1, PAX1, PTPRR, SOX1, and ZNF582, previously found from squamous cell carcinomas in adenocarcinomas (ACs) of the uterine cervix. Methods We assessed the methylation status of these genes in 40 ACs, cervical scrapings from 23 ACs, and 67 normal control cervices by real-time quantitative methylation-specific polymerase chain reaction. The results were validated by bisulfite pyrosequencing. Results The methylation levels of all the 6 genes in the ACs were significantly higher than those in normal cervical tissues, especially for PAX1, PTPRR, SOX1, and ZNF582. The odds ratios and 95% confidence intervals (CIs) of high methylation levels in PAX1, PTPRR, SOX1, and ZNF582 for the risk of developing an AC were 15.7 (95% CI, 7.0–40.6), 16.9 (95% CI, 7.6–43.0), 32.1 (95% CI, 12.1–124.3), and 25.4 (95% CI, 10.4–78.3), respectively (all P < 0.001). The methylation indices of PAX1, PTPRR, SOX1, and ZNF582 recovered from scrapings of ACs were significantly higher than in normal controls. The odds ratios of these indices for the risk of developing an AC in PAX1, PTPRR, SOX1, and ZNF582 were 6.2 (95% CI, 2.6–15.4), 12.1(95% CI, 3.8–46.4), 6.2 (95% CI, 2.6–15.8), and 20.6 (95% CI, 6.9–77.5), respectively (all P < 0.001). Conclusions Cervical ACs carry aberrantly high methylation rates of PAX1, PTPRR, SOX1, and ZNF582—commonly methylated in squamous cell carcinomas—which might help for AC screening.
Flexor Hallucis Longus Tendon Transfer for Hallux Claw Toe Deformity and Vertical Instability of the Metatarsophalangeal Joint

Background: Historically, operative treatment of hallux claw toe deformity has been the Jones procedure or one of its modifications. Review of the literature confirms its ability to maintain alignment and achieve patient satisfaction; however, several complications have been reported. Many of these are related to altered biomechanics across the metatarsophalangeal (MTP) joint. A similar approach to this clinical problem involves restoration of the flexion moment across the MTP joint without an interphalangeal (IP) joint arthrodesis. Methods: We conducted a retrospective review of 10 patients who had a flexor hallucis longus (FHL) tendon transfer to the proximal phalanx of the great toe for hallux claw toe deformity, as well as for symptomatic vertical instability. Four patients had a positive drawer test indicating vertical instability without static deformity. Two patients with vertical instability were found to have a dynamic deformity. Mean followup was 24 (SD 15.2) months. Results: All deformities were corrected and alignment was maintained at the time of followup. Pain under the first metatarsal head was reliably improved in symptomatic patients (p < 0.05). Patient satisfaction also was assessed. All six patients treated for hallux claw deformity were satisfied with their outcomes. Two of the four patients treated for vertical instability were satisfied. The other two patients expressed dissatisfaction because of persistent pain that occurred during strenuous exercise. Their symptoms during routine activity were improved, however. Conclusion: Based on initial results, the FHL transfer to the proximal phalanx appears to be a viable treatment option for hallux claw deformity in terms of deformity correction, pain relief, and patient satisfaction. Further evaluation is warranted regarding the indication of vertical instability.
Vertrauen und Betrug in Multi-Agenten Systemen : Erweiterung des Vertrauensmodells von Castelfranchi und Falcone um eine Kommunikationskomponente

Diese Arbeit beschaftigt sich mit betrugerischen Agenten in Kunstlichen Gesellschaften und damit, wie andere Agenten sich vor ihnen schutzen konnen. Zu diesem Zweck werden Agenten mit Berechnungsmodellen fur zwei Konzepte von "Vertrauen" ausgestattet. Zum einen berechnen sie Vertrauen in Interaktionspartner mit einer prazisierten Variante des Modells von Castelfranchi und Falcone. Zum anderen benutzen sie eine hier vorgestellte Form von Vertrauen, um mit anderen uber das Verhalten von unbekannten Agenten zu kommunizieren. Durch diesen Datenaustausch sind sie in der Lage, fremde Agenten wesentlich schneller und besser einzuschatzen. Mit diesem Wissen konnen sich Agenten effektiver vor betrugerischen und nicht-benevolenten Agenten schutzen. Das Vertrauen in Kommunikationspartner schafft einen "sozialen Kitt", uber den innerhalb einer Gruppe Informationen zuverlassig ausgetauscht werden konnen. Desweiteren wird hier das Offen Gespielte Gefangenendilemma mit Partnerauswahl vorgestellt. Dabei handelt es sich um ein spieltheoretisches Modell, in dem Agenten andere betrugen konnen. Diese Variation des Gefangenendilemmas dient als Experimentalumgebung fur heterogene Agentengesellschaften. Diese Experimentalumgebung besitzt wichtige Eigenschaften von Anwendungsszenarien wie z.B. die Kooperation in Virtuellen Markten. Sie ist so gestaltet, dass die Effektivitat von Strategien im Umgang mit betrugerischen Agenten untersucht werden kann. Dies bedeutet, dass mit ihrer Hilfe Turniere, ahnlich dem in der Literatur viel beachteten Turnier von Axelrod, durchgefuhrt werden konnen. Schlieslich wird diese Experimentalumgebung genutzt, um das hier vorgestellte Modell des Vertrauens in Kommunikationspartner in einer Reihe von Experimenten, in denen die Agenten kein a priori Wissen uber das Verhalten anderer haben, zu analysieren. Bei dieser Analyse werden Konfigurationen von verschieden ehrlichen und kooperationswilligen Agenten untersucht. In der Evaluation des Ansatzes zeigt sich, dass Agenten durch den Austausch von Wissen mit anderen vertrauenswurdigen Agenten ihre Interaktionspartner besser einschatzen konnen. Insbesondere sind sie in der Lage, Interaktionspartner einzuschatzen, die sie selbst noch nie beobachten konnten. In den untersuchten Agentengesellschaften bedeutet dies einen Performanzgewinn von mehr als funfzehn Prozent, ohne dass die Agenten ein a priori Wissen uber das Verhalten ihrer Interaktionspartner haben. Die Benutzung von Vertrauen und Kommunikation zahlt sich insbesondere dann aus, wenn nur wenige Beobachtungen uber das Verhalten anderer zur Verfugung stehen.
Antecedents and Impacts of Trust in Travel-Related Consumer-Generated Media

This study investigated the factors influencing trust in travel-related consumer-generated media (CGM) and the degree to which trust affects the benefits and impacts of using CGM when planning pleasure trips. An online survey of US Internet users was conducted with a total of 1,170 individuals responding to questions regarding their perceptions and use of CGM for travel planning. The findings show that CGM use is widespread and that trust depends on the type of website on which the CGM is posted, perceptions of other CGM creators as well as CGM readers' personality. Also, those who have greater trust in CGM report greater impacts and derive greater benefits from their CGM use.
More questions than answers. Commentary on Fuchs et al., examining novel concepts of the pathophysiology of depression in the chronic psychosocial stress paradigm in tree shrews.

Ever since I heard a lecture on the behavior and physiology of tupaias by Dietrich von Holst at an ethology congress more than 20 years ago, and learned about the adventurous introduction of tupaias from the South-East Asian archipelago to laboratories in Bavaria by the late Achim Raab, I have been fascinated by this species. Male tree shrews (Tupaia belangeri) may be one of the most intolerant mammals on this planet, vigorously marking, patrolling and defending their territory every day. It is no surprise that this socially intolerant species stars prominently in research on social stress. As in their many recent reviews, Fuchs et al. (2004) introduce the tupaias with a brief discussion of textbook-style stress physiology, including glucocorticoid receptors in the hippocampus. Many interesting issues are mentioned, and I will raise a few of these unresolved questions.
Paradigma de equivalência de estímulos norteando o ensino de rudimentos de leitura musical

Este estudo avaliou um procedimento para ensinar rudimentos de leitura musical baseado no procedimento de pareamento ao modelo e no paradigma de equivalencia de estimulos. Ensinaram-se, a quatro universitarias, relacoes entre sequencias de notas tocadas (A) e respectivas notacoes em Clave de Sol (B), Clave de Fa (C) e figura do teclado musical (D), com o procedimento de pareamento ao modelo. Na Fase 1, as sequencias ensinadas foram do-mi-sol e mi-sol-do e na Fase 2 , as sequencias foram Re-Fa-La e Fa-La-Re. Avaliou-se a emergencia das Relacoes BC, CB, BD, DB, CD e DC, e do desempenho de tocar teclado no final de cada fase. Todos os participantes aprenderam as 12 relacoes ensinadas. Os desempenhos em todos os testes aumentaram em cada fase e entre fases experimentais. Todas as participantes desenvolveram classes de estimulos equivalentes na Fase 2. Tres participantes mostraram leitura recombinativa e transferencia de controle de estimulos para tocar teclado. Os resultados confirmam a utilidade do procedimento de pareamento ao modelo e do paradigma de equivalencia para ensinar e avaliar a aprendizagem de repertorio simbolico complexo e podem contribuir para o desenvolvimento de tecnologia alternativa para ensinar leitura musical.
Polish Yellow Sweet Clover (Melilotus officinalis L.) Honey, Chromatographic Fingerprints, and Chemical Markers

A case study of Polish Melilotus officinalis honey was presented for the first time. Gas chromatography–mass spectrometry (GC-MS) (after steam distillation, Soxhlet extraction, ultrasonic solvent extraction, and solid phase extraction (SPE)) and targeted high performance liquid chromatography with a photodiode array detector (HPLC-PAD) were applied to determine the characteristic components of honey. While ubiquitous in most honeys, carbohydrates, terpene derivatives, and phenylacetic acid dominated in the Soxhlet extracts (25.54%) and in the application of SPE (13.04%). In addition, lumichrome (1.85%) was found, and may be considered as a marker of this honey. Due to the presence of these compounds, Polish yellow sweet clover honey is similar to French lavender honeys. The major compounds determined in the methanolic extract were (+)-catechine (39.7%) and gallic acid (up to 30%), which can be regarded as specific chemical markers of the botanical origin of melilot honey. With respect to total phenolic and flavonoid contents, 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were determined spectrophotometrically. The honey exhibited a moderate antioxidant activity, typical for light honeys, which correlates well with its phenolic and flavonoid composition.
The Canadian Rockies and Alberta Network (CRANE): New Constraints on the Rockies and Western Canada Sedimentary Basin

The Canadian Rockies and the neighboring Alberta Basin mark the transition from the old North American continental lithosphere (east) to young accreted “terranes” (west). Geologic, seismic, and magnetic data in this region have suggested complex crustal domains, conductive anomalies, and major seismic velocity gradients in the mantle. However, the nature of the boundaries between the basement domains and their vertical extents remains controversial due to the lack of exposed geology and limited seismic and electromagnetic receivers. Since 2006, the seismic data coverage and depth sensitivity have received a major boost from the establishment of the Canadian Rockies and Alberta Network (nicknamed CRANE), the first semi-permanent broadband seismic array in Alberta. The availability of the array data provides vital constraints on the regional micro-earthquakes and crust/mantle seismic structures. Among the broad range of ongoing efforts, this study highlights promising results from the analyses of P -to- S wave receiver functions, shear wave splitting amplitudes/directions, and ambient seismic noise. Our preliminary receiver-function stacks show that the base of the crust gradually shallows from approximately 60 km beneath the Rockies near the Canadian-U.S. border to 37–40 km beneath central Alberta; the latter range is consistent with earlier findings from active-source experiments. Converted waves from “littered” crust and/or lithosphere have also been detected at a number of stations in the depth range of 80–130 km. Complexities in the lithosphere are further evidenced by our regional shear wave splitting measurements. We infer a strong east-west change of mantle flow pattern, consistent with present-day plate motion. The spatial distribution of the SKS fast orientations highlights the contrasting crust/mantle structures and histories between the Rockies and adjacent domains. Dynamic effects associated with a migrating continental root east of the province may be important. Finally, our preliminary inversions using ambient seismic noise indicate more than 0.8 km/s in peak-to-peak group velocity variations throughout the crust. The upper crust beneath the Alberta Basin is dominated by low Rayleigh-wave group velocities. A lower-than-expected correlation between seismic velocities and tectonic domain boundaries suggests significant tectonic overprinting in the southern Western Canada Sedimentary Basin. Overall, the broadband seismic data from CRANE could play a key role in uncovering the mysteries of the crust and mantle beneath the transition region between cratons and terranes.