Miracle-dz/UniFAR-FTUs huggingface dataset:
Probing the role of structural features of mouse PrP in yeast by expression as Sup35-PrP fusions

The yeast Saccharomyces cerevisiae is a tractable model organism in which both to explore the molecular mechanisms underlying the generation of disease-associated protein misfolding and to map the cellular responses to potentially toxic misfolded proteins. Specific targets have included proteins which in certain disease states form amyloids and lead to neurodegeneration. Such studies are greatly facilitated by the extensive ‘toolbox’ available to the yeast researcher that provides a range of cell engineering options. Consequently, a number of assays at the cell and molecular level have been set up to report on specific protein misfolding events associated with endogenous or heterologous proteins. One major target is the mammalian prion protein PrP because we know little about what specific sequence and/or structural feature(s) of PrP are important for its conversion to the infectious prion form, PrPSc. Here, using a study of the expression in yeast of fusion proteins comprising the yeast prion protein Sup35 fused to various regions of mouse PrP protein, we show how PrP sequences can direct the formation of non-transmissible amyloids and focus in particular on the role of the mouse octarepeat region. Through this study we illustrate the benefits and limitations of yeast-based models for protein misfolding disorders.
Double-layer hepatocyte tumor co-culture using hydrogel for drug effectivity and specificity analysis

This paper presents the formation of double-layer cellular tumor spheroid for the co-culture of hepatocyte (HepG2 cancer liver cell and LO2 normal liver cell) using alginate hydrogel. With the microfluidic chip, double-layer cellular tumor spheroid can be formed by the hydrogel droplet to mimic the in vivo environment in which the cancer cell is surrounded by normal cell. A microchip with concentration gradient generation and tumor spheroid trapping site is designed for anti-cancer drug analysis.
Insights the Practice of Integrated Reporting: A Study on MNCs in Bangladesh on the Degree of Adherence to the Reporting Framework

Integrated Reporting (IR) stands for organizational reporting which is prepared for public disclosure and includes both financial and important non-financial information. Existing financial reporting standards are inadequate to address issues like the importance of intangible assets, corporate impacts on the environment, human health, societal conditions and corporate influence on the political process. Thus, the concept of IR emerged to deal with these issues that affect corporate success. The traditional reporting model focuses on a relatively narrow account of historical financial performance of the value-creation process. Keeping this in mind, the International Integrated Reporting Council (IIRC) has developed an International Integrated Reporting Framework, the core objective of which is to guide organizations in communicating the broad set of information needed by investors and other stakeholders to assess the organization’s long-term prospects in a clear, concise, connected and comparable format. The IIRC shares the view that the evolution of corporate reporting should be led by the communication of value creation by the corporate entities. This paper focuses on the degree of disclosure of Integrated Reporting requirements by the top eleven multinational companies of Bangladesh as per market capitalization. Using annual report content analysis, the findings show that the companies have lately started providing non-financial information regarding environment, society and governance along with financial figures. But it is prominent that they are still providing these information in disconnected strands and as a part of Corporate Governance or CSR disclosures instead of linking these to financial information and providing it as an integrated report.
Co-creators in innovation ecosystems. Part I: The case of creative industries

The continuous evolution and development of technology became the main driver for new industries emergence. The use of Internet of Things and Open Innovation technologies brought a new perspective regarding business models development, inter-industry collaboration, and value co – creation process. This point of view emphasizes the relevance and necessity for identification and analysis of business ecosystems concept. The transition from supply chain to value network, from business ecosystem to innovation one emphasized the importance of customer as value creator and the efficient use of other stakeholders’ capabilities. From this point of view, in order to create a competitive advantage, economic actors developed their own co–creation instruments including co–creation platforms. Based on literature review there will be highlighted the most relevant key aspects regarding the creation of an innovation ecosystem, distribution of innovation across the entire business landscape, the processes which facilitate the use of open innovation techniques and technologies, and the particularities of crowdsourcing within innovation ecosystems. The foreseen result is to explore the creation of an innovation ecosystem based on the experience of Crowdspring projects within an emerging creative industry, to identify major actors and the established relations, and to illustrate the use of crowdsourcing as the main open innovation technique.
The European Large Subunit Ribosomal RNA Database

The European Large Subunit (LSU) Ribosomal RNA (rRNA) database is accessible via the rRNA WWW Server at URL http://rrna.uia. ac.be/lsu/. It is a curated database that compiles complete or nearly complete LSU rRNA sequences in aligned form, and also incorporates secondary structure information for each sequence. Taxonomic information, literature references and other information about the sequences are also available, and can be searched via the WWW interface.
ISLAND AREA AND BODY SIZE OF INSULAR MAMMALS: EVIDENCE FROM THE TRI‐COLORED SQUIRREL (CALLOSCIURUS PREVOSTI) OF SOUTHEAST ASIA

The presence of Pleistocene elephants on Crete only 1 m in shoulder height, of giant rodents on Caribbean Islands, and of other equally remarkable dwarf and giant mammals, has prompted a variety of responses from their startled discoverers. Most of the mechanisms suggested for their origins are simplistic-usually a single factor, ranging from extreme food deprivation to isolation of antediluvian species. To my knowledge, no one has described adequately either the pattern of variation of mammals on islands, or synthesized the many suggested causes (Van Valen, 1973). This paper is an initial attempt to redress this deficiency. It is not intended as a thorough review; rather, it presents significant new data, briefly summarizes trends in body size of mammals, and presents a causal model of body size evolution in insular mammals. It is possible that the processes and patterns discussed here apply to other animals but I have directed my studies to maimmals alone. Each topic discussed below is worthy of a lengthy review, and it is my hope that this paper will stimulate additional research and discussion. During a study of the systematics of Callosciurus prevosti, the tri-colored squirrel of Malaysia, Indonesia, and peninsular Thailand, it became evident that body size of these squirrels was related to the size of the island on which the squirrels lived. This observation stimulated a more detailed study of size variation in these squirrels and led to the present paper. Body size of C. prevosti is discussed at the beginning of the paper, because variation in this species provided insight into the evolution of body size of insular mammals and formed the basis of my hypotheses and models. The biology of C. prevosti is poorly known. Externally tri-colored.squirrels resemble the familiar North American tree squirrels but are more brightly colored. Most populations have a black dorsum and tail, a broad white lateral stripe, and a red venter; many have a prominent shoulder patch which may be black, gray, red, white, or a mixture of these. In Selangor, Malaysia, tri-colored squirrels nested in trees and fed on coconut and rubber seeds, and oil palm fruit (Harrison and Traub, 1950; Harrison, 1962). Davis (1962) stated that in Sabah, North Borneo, they were found in primary and old logged forest where they inhabited chiefly the smaller trees of the middle story. Stomach contents consisted of pulpy fruit and about 5% insect matter, chiefly ants and termites. Three pregnant females taken on 20 June, 24 June, and 2 August had two, two, and three embryos, respectively. At Bukit Lanjong, West Malaysia, they have been seen feeding on the fruit of fig trees (Ficus) in the upper story (MacKinnon and MacKinnon, 1974:36). Nothing definite is known of the predators or competitors of these squirrels. ' Parts of this study were carried out while the author was resident at the Division of Mammals, Smithsonian Institution, Washington, D.C. 20560, and the Bell Museum of Natural History, University of Minnesota, Minneapolis, Minnesota 55455.
Learning Rate Updating Methods Applied to Adaptive Fuzzy Equalizers for Broadband Power Line Communications

This paper introduces adaptive fuzzy equalizers with variable step size for broadband power line (PL) communications. Based on delta-bar-delta and local Lipschitz estimation updating rules, feedforward, and decision feedback approaches, we propose singleton and nonsingleton fuzzy equalizers with variable step size to cope with the intersymbol interference (ISI) effects of PL channels and the hardness of the impulse noises generated by appliances and nonlinear loads connected to low-voltage power grids. The computed results show that the convergence rates of the proposed equalizers are higher than the ones attained by the traditional adaptive fuzzy equalizers introduced by J. M. Mendel and his students. Additionally, some interesting BER curves reveal that the proposed techniques are efficient for mitigating the above-mentioned impairments.
Treatment of Benign Chronic Gastric Ulcer with Ranitidine: A Randomized, Double‐blind, and Placebo‐Controlled Six Week Trial

A randomized, multicenter, double-blind, placebo-controlled study was conducted to determine whether ranitidine 150 mg b.i. d. for 6 weeks would expedite endoscopic healing or relief of symptoms in patients with benign gastric ulcer. Of 203 patients enrolled, 101 received ranitidine and 102 received placebo. Endoscopic evaluations were conducted at baseline and at 2 and 6 weeks. At 6 weeks 68% of the patients treated with ranitidine had healed compared with 53% in the placebo group (p = 0.02). In those patients who had not healed by 6 weeks, ranitidine provided greater relief from pain than placebo. More patients in the placebo group dropped out of the study because of worsening symptoms (13 versus 4, p = 0.04). No differences in laboratory abnormalities or incidence of adverse events were detected between the two study groups. These results indicate that ranitidine 150 mg b.i. d. is superior to placebo in the treatment of benign gastric ulcer.
“ 3 D TSV Micro Cu Column Chip-to-Substrate / Chip Assmbly / Packaging Technology ”

Increasing demand for new and more advanced electronic products with a smaller form factor, superior functionality and performance with a lower overall cost has driven semiconductor industry to develop more innovative and emerging advanced packaging technologies. Memory bandwidth has become a bottleneck to processor performance and lower power consumption for high performance computing needs. To reduce obstacles, a revolution in device architecture and package technologies is require. 3D TSV (Through Silicon Via) stacking is believed to be one of the technologies that can meet those requirements. In advanced 3D stacking technologies, one of the important steps is to develop and assemble fine pitch, high density microbumps. This type of microbump in flip chip interconnection provides a high wiring density in silicon die with a high-performance signal and power connection. This paper addresses Cu column technology for 3D TSV assembly/packaging process. Latest developments in the key elements of thin TSV wafer dicing and thermocompression bonding with Cu column bumps are presented. 3D TSV interconnects as an interim play prior to full production of the active Si TSV approach is reviewed with specific example of configurations approaching volume production in real products. TSV backside via reveal process including thin wafer handling will be discussed. TSV Packaging challenges, thermocompression bonding and its reliability experimental results will be presented for CTC (Chip-toChip), CTS (Chip-to-Substrate) bonding with ultra fine 40um pitch microbump interconnections in this paper.
Bacteremia in febrile children seen in a "walk-in" pediatric clinic.

Abstract In an effort to identify high-risk children blood for culture was obtained in children whose temperature was 38.3°C or higher when they were seen in the pediatric "walk-in" clinic. Clinically important bacterial pathogens were identified in the blood from 31 of 708 children in a three-month period. Diplococcus pneumoniae and Haemophilus influenzae were the most frequent of seven species identified. The data suggest that in this setting, a blood culture provides valuable help in establishing the specific bacteriologic diagnosis in febrile children without focal signs of infection. Bacteremia was most frequent in children seven to 12 months old (p<0.001) and was associated with a white-cell count of 20,000 or more (p<0.01)and a temperature of 39.4°C or higher (p<0.01). (N Engl J Med 288:1309–1312, 1973)
Establish Cross-Border Warehousing and Improve the Competitiveness of E-Commerce

This thesis enhances the competitiveness of e-commerce by studying overseas warehousing and then supplements the existing research theories of global e-commerce. First, this paper summarizes the development of cross-border e-commerce and overseas warehousing supply chain models. Secondly, it analyzes the current situation of e-commerce global market scale and analyzes the characteristics of China's e-commerce market. In the second chapter, the research background of e-commerce and its impact on supply chain management is introduced. The Internet revolution has brought fundamental changes to the company's business and strategy. It also spawned a new sales model called "e-commerce" or "electronic trading. " In addition to the more complex global concepts of more complex, more efficient, and more preferred customers, companies need to improve service quality and should be able to maintain lower operating costs in order to maintain market competitiveness. In addition, this article also discusses another area of expansion of e-commerce - cross-border e-commerce. Cross-border e-commerce is an online sale of products through retail channels or other countries' e-commerce platforms, without the need to go to that country in the field, it has certain advantages. Finally, the discussion goes into e-commerce, proposes to establish an overseas warehouse model, shorten delivery time, and improve the competitiveness of Chinese e-commerce in developed countries.
Organ‐specific Autoimmunity Induced by Lymphopenia

In 1973 two groups reported the induction of organ-specific autoimmune diseases by T cell deprivation. Penhale and co-workers (Penhale et al. 1973) found that autoimmune thyroiditis could be induced in 5-week-old Wistar rats by thymeclomy and repeated low-dose irradiation. On the other hand, Nishizuka and colleagues (Nishizuka et al. 1973) made the fascinating observation that autoimmune thyroiditis spontaneously developed in mice following thymectomy alone, so long as the thymectomy was carried out in the neonatal period. Subsequently, neonatal thymectomy was shown to induce a wide variety of organ-specific autoimmune diseases in mice, with different strains showing differences in disease susceptibility. The overlap in autoimmune diseases in this mouse model is reminiscent of the overlap observed in the endocrine organ/stomach cluster of organ-specific autoimmune diseases of humans. From 1975 onwards a number of other manipulations of the immune system have been shown to induce organ-specific autoimmune diseases in rodents. A common characteristic of these manipulations is either a deficit of T cells or reduction of the TCR repertoire, in other words functional T cell lymphopenia. The range of autoimmune diseases induced by these manipulations is most impressive and includes diabetes, thyroditis, gastritis, orchitis, oophoritis, prostatitis, and coagulating adenitis. Many of these diseases are rare in laboratory strains of rats and mice. Evidence that the autoimmune diseases are due to specific immune responses is supported by the presence of infiltrating lymphocytes in the target organ, circulat-
Nouvelles architectures de mémoires embarquées compatibles CMOS

Les systemes electroniques embarques constituent un enjeu majeur de l'industrie microelectronique. Ils sont traditionnellement constitues de cellules memoires DRAM (Dynamic Random Access Memory) a capacite de stockage co-integrees avec les fonctions logiques. Neanmoins la viabilite de cette cellule DRAM est fortement remise en jeu pour les generations CMOS (Complementary Metal Oxide Semiconductor) sub-90 nm. Ce travail de these contribue a l'evaluation et au developpement de cellules memoires alternatives et innovantes (sans capacite), fonctionnelles aux petites dimensions (10-100 nm). La premiere partie de ce rapport est dediee a l'etude de l'effet de substrat flottant observe sur les transistors MOS a triple-caisson. Une modelisation des phenomenes mis en jeux est proposee et verifiee experimentalement entre 25 et 100ʿC. Nous demontrons que cet effet est exploitable comme effet memoire pour les prochaines generations de DRAM embarquees (90-45 nm). Au cours de la seconde partie, les performances d'une architecture inedite, la " bascule DRAM ", sont evaluees du point de vue theorique et son procede de fabrication est developpe.
Dynamics of coherent optical phonons in a Bi2Se3 crystal

Coherent A<sub>1g</sub><sup>1</sup>, A<sub>1g</sub><sup>2</sup>, and E<sub>g</sub><sup>2</sup> phonons in Bi<sub>2</sub>Se<sub>3</sub> are observed using ultrafast spectroscopy. Their initial phases suggest different coupling strengths with photo-excited electrons. Their decay rates per oscillations are almost the same.
Detecting local adaptation in widespread grassland species – the importance of scale and local plant community

1 Adaptation of plant populations to local environments has been shown in many species but local adaptation is not always apparent and spatial scales of differentiation are not well known. In a reciprocal transplant experiment we tested whether: (i) three widespread grassland species are locally adapted at a European scale; (ii) detection of local adaptation depends on competition with the local plant community; and (iii) local differentiation between neighbouring populations from contrasting habitats can be stronger than differentiation at a European scale. 2 Seeds of Holcus lanatus, Lotus corniculatus and Plantago lanceolata from a Swiss, Czech and UK population were sown in a reciprocal transplant experiment at fields that exhibit environmental conditions similar to the source sites. Seedling emergence, survival, growth and reproduction were recorded for two consecutive years. 3 The effect of competition was tested by comparing individuals in weeded monocultures with plants sown together with species from the local grassland community. To compare large‐scale vs. small‐scale differentiation, a neighbouring population from a contrasting habitat (wet‐dry contrast) was compared with the ‘home’ and ‘foreign’ populations. 4 In P. lanceolata and H. lanatus, a significant home‐site advantage was detected in fitness‐related traits, thus indicating local adaptation. In L. corniculatus, an overall superiority of one provenance was found. 5 The detection of local adaptation depended on competition with the local plant community. In the absence of competition the home‐site advantage was underestimated in P. lanceolata and overestimated in H. lanatus. 6 A significant population differentiation between contrasting local habitats was found. In some traits, this small‐scale was greater than large‐scale differentiation between countries. 7 Our results indicate that local adaptation in real plant communities cannot necessarily be predicted from plants grown in weeded monocultures and that tests on the relationship between fitness and geographical distance have to account for habitat‐dependent small‐scale differentiation. Considering the strong small‐scale differentiation, a local provenance from a different habitat may not be the best choice in ecological restoration if distant populations from a more similar habitat are available.
Intervention strategies in relationship marketing : a competency framework for business partnerships

Traditional concepts of ‘marketing management’ intuitively reflect the notion of ‘intervention’, whether it is developing a new product to exploit a particular market niche or more generally intervening in markets to achieve a sustainable competitive advantage. However, in ‘relationship marketing’ the discussion of ‘intervention strategy’ is less precisely defined. Contributing to this imprecision is the lack of an overall cohesive framework for understanding ‘relationship marketing’ processes and the mechanisms which drive these processes. This paper seeks to advance our understanding of relationship marketing processes with special reference to business-to-business partnering, the mechanisms which drive these processes and the implications for ‘intervention strategy’ with respect to ‘alliance competence’ and ‘alliance structural and governance issues within the context of a quantitative study of 163 Australian based business-to-business partnerships.