Miracle-dz/UniFAR-FTUs huggingface dataset:
Functional proteomic analysis of GS‐NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate

We previously compared changes in individual protein abundance between the proteomes of GS‐NS0 cell lines with varying rates of cell‐specific recombinant monoclonal antibody production (qMab). Here we extend analyses of our proteomic dataset to statistically determine if particular cell lines have distinct functional capabilities that facilitate production of secreted recombinant Mab. We categorized 79 proteins identified by mass spectrometry according to their biological function or location in the cell and statistically compared the relative abundance of proteins in each category between GS‐NS0 cell lines with varying qMab. We found that the relative abundance of proteins in ER chaperone, non‐ER chaperone, cytoskeletal, cell signaling, metabolic, and mitochondrial categories were significantly increased with qMab. As the GS‐NS0 cell line with highest qMab also had an increased intracellular abundance of unassembled Mab heavy chain (HC), we tested the hypothesis that the increased ER chaperone content was caused by induction of an unfolded protein response (UPR) signaling pathway. Immunoblot analyses revealed that spliced X‐box binding protein 1 (XBP1), a marker for UPR induction, was not detectable in the GS‐NS0 cells with elevated qMab, although it was induced by chemical inhibitors of protein folding. These data suggest that qMab is functionally related to the abundance of specific categories of proteins that together facilitate recombinant protein production. We infer that individual cells within parental populations are more functionally equipped for high‐level recombinant protein production than others and that this bias could be used to select cells that are more likely to achieve high qMab. © 2006 Wiley Periodicals, Inc.
SUCCESSFUL MAINTENANCE HEMODIALYSIS IN NONAGENARIANS WITH END‐STAGE RENAL DISEASE AND PREEXISTING COMORBIDITIES

parents (44.4%) and others (8.4%). The recipients’ principal physical conditions were bedridden due to stroke with or without dementia (56.6%), dementia (26.4%), and others (17.0%). The murders were performed by strangulation (67.0%), stabbing (13.2%), striking (4.7%), and others (15.1%). The present study demonstrated that the current prevalence of murder by exhausted caregivers of frail recipients is not decreasing but rather seems to be increasing in Japan. Seven years have passed since the inception of the promising insurance system, and it seems necessary to ask whether the program has developed a fair and appropriate way of allocating limited resources to people with different diagnosis. Services are allocated based on the GovernmentCertified Disability Index. Recent reports suggest that caregiver burden was not worse after the public LTC insurance scheme took over in Japan, although it was reported that physical and psychological depression existed in caregivers before and after the introduction of the insurance system. Although the induction of Japan’s new LTC system was significant in making clear the plight of elderly people and their caregivers, the system should be improved to lighten the caregivers’ burden more. A government agency should be created to support not only frail elderly people, but also their caregivers as soon as possible.
The Back Pain of Bus Drivers: Prevalence in an Urban Area of California

A stratified random sample of 195 subjects was selected from the membership of an urban transit union in California, two thirds consisting of motor coach operators and one third serving as a nondriving comparison group. Based on an orthopaedic medical history and physical examination 80.5% of drivers were found to be experiencing back or neck pain at the time of examination, in contrast with 50.7 percent of nondrivers, itself a sizable percentage. For both groups, most pain was mild, (53.9 and 29.9%, respectively). The amount of severe pain was essentially the same in the two groups (10.2% and 9.0%). Both groups were most subject to low back pain. Drivers were most distinctive for movement-related pain in the cervical spine. They were also more subject in any part of the spine to postural pain. The latter finding suggests the need to introduce ergonomic and scheduling changes in the work of operating a motor coach. Drivers more than nondirvers struggled against their pain by doing exercise, seeking professional treatment, and taking medications, indicating that spinal disorders constitute a prominent health concern for workers in this occupation.
Cognitive styles: essence and origins. Field dependence and field independence.

ISBN: 0823610039 ISBN13: 978-0823610037 Author: Herman A. Witkin Book title: Cognitive Styles, Essence and Origins: Field Dependence and Field Independence (Psychological Issues) Pages: 151 Publisher: Intl Universities Pr Inc (June 1, 1981) Language: English Category: Psychology and Counseling Size PDF version: 1589 kb Size ePUB version: 1423 kb Size FB2 version: 1539 kb Other formats: docx azw doc docx
Basal replication of hepatitis C virus in nude mice harboring human tumor

Hepatitis C virus (HCV) can infect and propagate in humans and chimpanzees. Whereas the chimpanzee has been used as an animal model for infection, ethical considerations, conservation, and the prohibitively high cost preclude progress for experimental research on the biology of the virus. The development of a small animal model for HCV infection is thus desirable to facilitate studies on the infectious cycle of the virus and for the evaluation of drugs for the treatment of HCV infections in humans. As an alternative to the chimpanzee model, we have established a model based on ex vivo infection of orthotopically‐implanted human hepatocellular carcinoma cells (HCC) in athymic nude mice. The results show that up to 42 days post‐infection, HCV RNA was present in the tumor cells as well as in the liver and serum of infected mice. Furthermore, a direct correlation between size of the tumor and the presence of HCV RNA in the liver was observed, which is concordant with the finding that HCV RNA was detectable only in mice harboring human tumor. Immunohistochemistry analysis of infected liver specimens showed cells expressing the HCV encoded NS5B protein. A few mice developed a humoral response against the nonstructural viral proteins, providing further evidence for expression of these proteins during viral infection. In summary, these results suggest that mice harboring orthotopic tumors support a basal level of HCV replication in vivo. J. Med. Virol. 66:312‐319, 2002. © 2002 Wiley‐Liss, Inc.
Comparison of wound healing using the CO2 laser at 10.6 microm and 9.55 microm.

OBJECTIVES/HYPOTHESIS The wound healing characteristics of incisions made with the short pulsed CO2 laser tuned to 9.55 microm versus the traditional 10.6 microm were investigated. Previous studies have shown that at 9.55 microm, collagen is targeted more selectively than at 10.6 microm, which results in decreased acute thermal injury patterns. This study investigates the difference in wound healing over time between lasers and compares laser incisions with cold knife techniques. STUDY DESIGN Randomized controlled trial using a porcine model. METHODS Tissue from 10.6-microm and 9.55-microm incisions of 10 piglets was evaluated with histological analysis and tensiometry at 3, 7, 14, and 21 days postoperatively. A Bonferroni-Dunn corrected analysis of variance analysis at a 95% significance level was used to compare the effect of wavelength. RESULTS The results demonstrate that although knife incisions are consistently stronger than laser incisions, the 9.55-microm CO2 laser incisions are no stronger than incisions made with the conventional 10.6-microm laser. Furthermore, histological analysis shows no difference in lateral thermal damage between lasers at 3, 7, 14, and 21 days postoperatively. The progression of collagen formation and inflammation does not differ over time. CONCLUSION This study of wound healing using a porcine model demonstrates that the 9.55-microm CO2 laser does not demonstrate an improvement in wound healing over the traditional 10.6-microm CO2 laser. These results may be secondary to the common explosive vaporization mechanism produced by both lasers in the infrared spectrum.
Successful Combination of Nucleic Acid Amplification Test Diagnostics and Targeted Deferred Neisseria gonorrhoeae Culture

ABSTRACT Nucleic acid amplification tests (NAATs) are recommended for the diagnosis of N. gonorrhoeae infections because of their superior sensitivity. Increasing NAAT use causes a decline in crucial antimicrobial resistance (AMR) surveillance data, which rely on culture. We analyzed the suitability of the ESwab system for NAAT diagnostics and deferred targeted N. gonorrhoeae culture to allow selective and efficient culture based on NAAT results. We included patients visiting the STI Clinic Amsterdam, The Netherlands, in 2013. Patient characteristics and urogenital and rectal samples for direct N. gonorrhoeae culture, standard NAAT, and ESwab were collected. Standard NAAT and NAAT on ESwab samples were performed using the Aptima Combo 2 assay for N. gonorrhoeae and C. trachomatis. Two deferred N. gonorrhoeae cultures were performed on NAAT-positive ESwab samples after storage at 4°C for 1 to 3 days. We included 2,452 samples from 1,893 patients. In the standard NAAT, 107 samples were N. gonorrhoeae positive and 284 were C. trachomatis positive. The sensitivities of NAAT on ESwab samples were 83% (95% confidence interval [CI], 75 to 90%) and 87% (95% CI, 82 to 90%), respectively. ESwab samples were available for 98 of the gonorrhea-positive samples. Of these, 82% were positive in direct culture and 69% and 56% were positive in the 1st and 2nd deferred cultures, respectively (median storage times, 27 and 48 h, respectively). Deferred culture was more often successful in urogenital samples or when the patient had symptoms at the sampling site. Deferred N. gonorrhoeae culture of stored ESwab samples is feasible and enables AMR surveillance. To limit the loss in NAAT sensitivity, we recommend obtaining separate samples for NAAT and deferred culture.
Advanced Cu/low-k (k=2.2) multilevel interconnect for 0.10/0.07 /spl mu/m generation

A spin-on dielectric (SOD, k=2.2) has been integrated with Cu for 0.10/0.07 /spl mu/m generations. To minimize interconnect capacitance, conventional CVD cap layer (k=4.5-7.5) is replaced by a SOD dielectric (k=2.9) and no stop layer for trench etch is used for the porous inter-metal dielectric (IMD). The issue of photoresist poisoning is resolved by nitrogen-free IMD processing. Using polymeric abrasive together with polishing parameters designed in a low friction domain for planarization, 6-level Cu/porous SOD multilevel interconnect is demonstrated for the first time. Electrical testing shows promising results for the high-performance dual damascene structure.
Complete Genome Sequence of the Cellulolytic Thermophile Clostridium thermocellum DSM1313

ABSTRACT Clostridium thermocellum DSM1313 is a thermophilic, anaerobic bacterium with some of the highest rates of cellulose hydrolysis reported. The complete genome sequence reveals a suite of carbohydrate-active enzymes and demonstrates a level of diversity at the species level distinguishing it from the type strain ATCC 27405.
How to flourish less developed areas' e-businesses: Focusing on free shipping effect

Shipping cost is a crucial factor that influences people's online purchasing behaviors. Free shipping option can increase customers' behavior greatly while shipping cost even only occupy a small amount of the whole price will impede purchasing behaviors obviously. If all e-business retailers in one area have the ability to provide free shipping options to all online customers, the e-business in this area will win out other areas in competition and the e-economy of this area will be flourished. But in a natural status, free shipping option emerges slowly and unevenly. This situation provides local governments of less-developed areas a chance to promote their e-business economies. This paper elaborates the conduct mechanism based on supply chain and behavioral economics. The mechanism is divided into customers make purchasing decisions, local online retailers provide free shipping options, express companies receive transfer payments and local government takes active interventions. East China's e-business data supports most parts of this viewpoint. It is concluded that free shipping effect can be gained along the chain of customers, retailers, express companies and local government. Less developed area's local governments can adopt active interventions upon express companies to stimulate e-economy in their areas.
Scale-dependent method for whole arm grasp evaluation

This paper presents a novel scale-dependent method for grasp evaluation based on so-called size ratio (introduced in this paper) and the ability of the grasp to reject disturbance forces. Manipulating large objects, comparable to the robot size is considered. This is the case of a whole arm manipulation (WAM) in which the robot arm can manipulate larger objects as the whole surface is used. In this way task-oriented information is incorporated in proposed grasp evaluation criterion. Examples are presented to demonstrate the validity of the proposed approach.
On the Interpretability of Thresholded Social Networks

Understanding the factors of network formation is a fundamental aspect in the study of social dynamics. Online activity provides us with abundance of data that allows us to reconstruct and study social networks. Statistical inference methods are often used to study network formation. Ideally, statistical inference allows the researcher to study the significance of specific factors to the network formation. One popular framework is known as Exponential Random Graph Models (ERGM) which provides principled and statistically sound interpretation of an observed network structure. Network, however, are not always given set in stone. Often times, a network is “reconstructed” by applying some thresholds on the observed data/signals. We show that subtle changes in the thresholding have significant effects on the ERGM results, casting doubts on the interpretability of the model. In this work we present a case study in which different thresholding techniques yield radically different results that lead to contrastive interpretations. Consequently, we revisit the applicability of ERGM to thresholded networks.
A study of multifrequency polarization pulse profiles of millisecond pulsars

We present high signal-to-noise ratio, multifrequency polarization pulse profiles for 24 millisecond pulsars that are being observed as part of the Parkes Pulsar Timing Array project. The pulsars are observed in three bands, centred close to 730, 1400 and 3100 MHz, using a dual-band 10 cm/50 cm receiver and the central beam of the 20-cm multibeam receiver. Observations spanning approximately six years have been carefully calibrated and summed to produce high S/N profiles. This allows us to study the individual profile components and in particular how they evolve with frequency. We also identify previously undetected profile features. For many pulsars we show that pulsed emission extends across almost the entire pulse profile. The pulse component widths and component separations follow a complex evolution with frequency; in some cases these parameters increase and in other cases they decrease with increasing frequency. The evolution with frequency of the polarization properties of the profile is also non-trivial. We provide evidence that the pre- and post-cursors generally have higher fractional linear polarization than the main pulse. We have obtained the spectral index and rotation measure for each pulsar by fitting across all three observing bands. For the majority of pulsars, the spectra follow a single power-law and the position angles follow a lambda(2) relation, as expected. However, clear deviations are seen for some pulsars. We also present phase-resolved measurements of the spectral index, fractional linear polarization and rotation measure. All these properties are shown to vary systematically over the pulse profile.
The toxicity of a ternary biocide mixture to two consecutive earthworm (Eisenia fetida) generations

The aim of the present study was to determine the toxicity of a mixture containing the biocides picoxystrobin, esfenvalerate, and triclosan to the reproduction and adult survival of two consecutive generations of Eisenia fetida (Savigny, 1826). Concentration addition and independent action were used to predict mixture toxicity. Due to degradation of mixture components during the course of the experiment, predictions were based both on the mixture composition at the beginning and the end of the exposure period. As degradations were dose–dependent, none of the calculated predictions were precise for the entire concentration range, although combining both predictions led us to conclude that lethal toxicity was well predicted by concentration addition and sublethal toxicity by independent action. Reproduction of the F1 generation was inhibited more (p < 0.0001) than reproduction of the F0 generation. Adult survival did not differ between generations. The accuracy of the mixture toxicity predictions thus depended on both the time‐dependent mixture composition and the earthworm generation. The results of this study underline the need for more advanced mixture toxicity prediction models that consider degradation kinetics and changes in toxic effects over time. Environ. Toxicol. Chem. 2013;32:937–947. © 2013 SETAC
A Sensitive Gel-based Method Combining Distinct Cyclophellitol-based Probes for the Identification of Acid/Base Residues in Human Retaining β-Glucosidases*

Background: Cyclophellitol-derived activity-based probes label the nucleophile of retaining β-glucosidases by exploiting their catalytic mechanism. Results: Combining two distinct cyclophellitol-derived probes with azide-mediated activity-rescue of mutated retaining β-glucosidases enables acid/base residue identification. Conclusion: A sensitive, new gel-based method for catalytic residue identification is developed. Significance: Cyclophellitol-based probes are proposed as useful tools for the identification of catalytic residues in retaining glycosidases. Retaining β-exoglucosidases operate by a mechanism in which the key amino acids driving the glycosidic bond hydrolysis act as catalytic acid/base and nucleophile. Recently we designed two distinct classes of fluorescent cyclophellitol-type activity-based probes (ABPs) that exploit this mechanism to covalently modify the nucleophile of retaining β-glucosidases. Whereas β-epoxide ABPs require a protonated acid/base for irreversible inhibition of retaining β-glucosidases, β-aziridine ABPs do not. Here we describe a novel sensitive method to identify both catalytic residues of retaining β-glucosidases by the combined use of cyclophellitol β-epoxide- and β-aziridine ABPs. In this approach putative catalytic residues are first substituted to noncarboxylic amino acids such as glycine or glutamine through site-directed mutagenesis. Next, the acid/base and nucleophile can be identified via classical sodium azide-mediated rescue of mutants thereof. Selective labeling with fluorescent β-aziridine but not β-epoxide ABPs identifies the acid/base residue in mutagenized enzyme, as only the β-aziridine ABP can bind in its absence. The Absence of the nucleophile abolishes any ABP labeling. We validated the method by using the retaining β-glucosidase GBA (CAZy glycosylhydrolase family GH30) and then applied it to non-homologous (putative) retaining β-glucosidases categorized in GH1 and GH116: GBA2, GBA3, and LPH. The described method is highly sensitive, requiring only femtomoles (nanograms) of ABP-labeled enzymes.
On the Expressiveness of Fixed-Priority Scheduling Contexts for Mixed-Criticality Scheduling

Scheduling contexts allow flattening hierarchical schedules in virtualized mixed-criticality setups. However, their expressiveness in terms of supported higher-level scheduling algorithms is not yet well understood. This paper makes a first step in this direction by investigating how recently proposed mixed-criticality algorithms can be mapped to fixed-priority scheduling contexts and how scheduling contexts can be extended to support these algorithms. We found that although the initial implementation of scheduling contexts was rather limited, a few practically feasible extensions broadened their applicability to all investigated algorithms.
Caregiver Perspectives on Transitions to Assisted Living and Memory Care

This study describes family caregivers’ experiences moving relatives with Alzheimer’s disease or related disorders (ADRD) from their homes to assisted living facilities (ALFs) and subsequently to memory care units (MCUs). We also examined how these experiences differed between caregiver dyad types, such as adult children caring for parents. In-depth interviews with 15 caregivers were transcribed verbatim. Grounded theory identified themes. Constant comparative analysis compared experiences of caregiver dyads. Most caregivers recognized the likely need for future specialized care at the time of the move to the ALF, but did not recall receiving information about transfer policies. The ALF move was harder for spouses, the MCU move for adult children. Assisted living facilities can improve support for caregivers facing a relative’s MCU transition through education about advantages of MCU placement and information about transfer policies. Support needs during transitions may differ between adult children and spouses.
Effects of Theobromine and Caffeine on Mood and Vigilance

Abstract Like caffeine, theobromine crosses the blood-brain barrier and binds to adenosine receptors, suggesting it might share caffeine’s beneficial effects on mood and vigilance. Therefore, the purpose of this study was to assess the effect of theobromine doses commonly found in foods on mood and vigilance parameters sensitive to caffeine. Caffeine was tested as a positive control. Twenty-four men (age, 23 [3] years) completed 6 double-blind trials during which they consumed experimental beverages, assessed their mood using standardized self-report questionnaires, and completed a 2-hour visual vigilance task. Three experimental doses (100, 200, and 400 mg theobromine) were delivered in a cocoa-based beverage; 3 matched control treatments (0 mg theobromine, 400 mg theobromine, and 100 mg caffeine) were delivered in a non–cocoa beverage. Mean salivary concentrations of theobromine exhibited significant dose-dependent differences (400 mg trials > 200 mg trial > 100 mg trial > 0 mg trials; P < 0.005). At every dose tested, theobromine failed to consistently affect mood state or vigilance (P > 0.05), but 100-mg caffeine significantly decreased lethargy/fatigue and increased vigor (P = 0.006 and 0.011, respectively). These findings indicate theobromine does not influence mood and vigilance when administered in nutritionally relevant doses, despite sharing many of caffeine’s structural characteristics.
Selection of Coordination Mechanisms in Intelligent Environments

This research work is about the choice of a coordination mechanism, essentially to allow migration of users and services, among multi-agent systems. Current approaches to coordination multiagent systems have addressed the problem of coordination, but only among the agents in a society of agents, leaving open the question of coordination among societies of agents. The purpose of this research is to propose a Framework for helping to choose a coordination mechanism for the integration of multi-agent systems in an intelligent ecosystem. To show our proposal, we focus our attention in a ecosystem of intelligent environments oriented towards medical monitoring as a use case.