Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of Solvents on Phytoconstituents and Antimicrobial Activities of Ocimum gratissimum and Eugenia caryophyllata Extracts on Listeria monocytogenes

Bu calismada farkli solventlerin aktif maddeler ile Ocimum gratissimum ’ un taze yapraklari ve Eugenia caryophyllata ’ nin kuru tomurcuklarinin antimikrobial performanslari uzerindeki etkileri arastirildi. Bitki parcaciklari soxhlet cihazi icerisinde dietil eter, etil asetat ve etanol kullanilarak ekstrakte edildi. Bitkilerin fito-bilesenleri, gaz kromatografisi- kutle spektrometresi (GC-MS) kullanilarak saptandi. Yuksek ojenol konsantrasyonlu ekstrakt 7 islemde hazirlandi (160, 80, 40, 20, 10, 5, and 0 mg/mL) ve Listeria monocytogenes ’ e karsi agar kuyucuk difuzyon metodu ile 3 kez tekrar edilerek test edildi. Minimum inhibitor konsantrasyonu (MIC) ve inhibisyon sahasi standart teknik kullanilarak tespit edildi. Veriler istatistiki olarak hesaplandi ve α 0.05 duzeyinde tek yonlu varyans analizi (ANOVA) yapildi. Sonuclara gore ekstraktin fito-kimyasal konsantrasyonu solventler ile iliskili olarak anlamli olcude (p<0,05) farklilik gosterdi. Etil asetat kullanilarak elde edilen E. caryophyllata ekstraktinin en yuksek (%71,32); O. Gratissimum ’ un ise %16,67 oraninda ojenol konsantrasyonu icerdigi gozlendi. Bulgulara gore, Ocimum gratissimum ’ un taze yapraklari ve Eugenia caryophyllata ’ nin kuru tomurcuklarinin sirasiyla 13,33 mm ve 19,00 mm ’ lik inhibisyon sahalari ile L. Monocytogenes ’ e karsi antimikrobiyel aktivite gosterdigi ve MIC degerinin 80 mg/mL oldugu belirlendi. Bu sonuclar, Ocimum gratissimum ’ un taze yapraklari ve Eugenia caryophyllata ’ nin kuru tomurcuklarinin gida kaynakli bakterilerin, ozellikle L. Monocytogenes ’ in gelisimini engellemek icin kullanabileceklerini kanitlamaktadir.
Destruction of bacteria using electric stimulation of old Duck and Chicken carcasses

The effect of electric stimulation on some quantitative characters of aged ducks and layers chicken carcasses were the main objectives of the present study. Electric stimulation apparatus of meat tenderness was designed and manufactured at Department of Food Sciences, College of Agriculture, University of Basrah. A total of 36 Ducks and Layers chicken aged 1.5 years. Birds were slaughtered by hand, were defeathered, and all internal organs were removed. Carcasses were divided into three treatments with different electric stimulation. Control group (no electric stimulation). The second treatment (Electric conductivity 3.67 V/cm) was low voltage of 110 volt and 1% saline solution. The third treatment (7.33 V/cm) was 220V and 1% saline solution. All traits were measured at 25 min, 6 hrs. and 24 hrs. Carcasses stored by freezing for 30 and 60 days. The results showed that the number of proteolysis, lipolysis, Psychrophiles bacteria were affected by electric stimulation, the third treatment revealed the lower number of bacteria. Electric stimulation (especially 220 V) was significantly reduced the number of bacteria in both chicken and duck.
DNA Damage Induced Cell Fates during Aging

Aging is a progressive loss of normal physiology, ultimately resulting in age-related pathology. One of the main drivers of aging is an accumulation of irreparable damage to the genome. This damage results in both an increase in cells undergoing apoptosis and an accumulation of senescent cells. These cell fates prevent oncogenesis at the expense of tissue homeostasis loss. A disproportional amount of apoptotic cells would severely damage tissue structure, while senescent cells have an altered secretion phenotype that reduces tissue function. We aimed to unravel the mechanisms and functions of age-related DNA damage responses to ultimately identify potential strategies that prolong healthspan during aging. We discovered that FOXO4 is progressively upregulated after senescence induction and is found in damage foci in senescent cells. Here, FOXO4 inhibits apoptosis through interacting with p53. We have developed a FOXO4-DRI peptide that blocks this interaction and thereby potently and selectively kills senescent cells, improving tissue homeostasis in fast aging XpdTTD/TTD and naturally aged mice. Furthermore, we have shown that this peptide can target cancer cells that exhibit senescence features, indicating that FOXO4-DRI has the potential to target the tumor micro-environment as well as the tumor itself. Besides senescence, we highlighted a micro-RNA signature that is upregulated during aging and inhibits DNA damage-dependent apoptosis in vitro. Furthermore, we showed that this signature is transferred to neighboring cells via extracellular vesicles. The miRNA targets and in vivo role were unknown and therefore we studied miR-30, a representative miRNA. MiR-30 inhibition proved to constitutively upregulate p53 expression, increasing apoptosis after UV-damage. This has potential detrimental effects during aging, since mouse models where p53 levels are increased show accelerated aging. Increasing cell preservation miRNAs may prevent apoptosis induction in cell types that are prone to cell death during aging. Overall this thesis illustrates how apoptosis and senescence underlie organismal aging and provides starting points for the development of potential therapeutic treatment for age-related pathology.
Occurrence of mycoparasitic fungi Sphaerellopsis paraphysata on pearl millet rust pathogen, Puccinia substriata in India

A mycoparasite was isolated on the rust infected pearl millet crop. It was isolated from an unusual pycnidia which was present along with the uredospores of rust. It reduced the uredial inoculum to a considerable extent. The morphology and molecular characters of the mycoparasite was studied. Its role as hyperparasite was established using Scanning Electron Micrographs, which clearly showed coiling and puncturing of uredospores of Puccinia substriata, by the mycelia of S. paraphysata. The confirmatory studies on the positive pathogenicity on rust fungi, showed a colourless septate mycelium, with an average conidial size of 9.27-12.92 ×2.55-5.87 µm and pycnidial size of 76.14-198.04×56.25-184.19 µm. The 540bp amplicon size of ITS sequence showed 96% similarity with S. paraphysata. The spore germination of Puccinia substriata on pearl millet leaves was reduced by 76%, 56%, 70% and the disease severity percentage was 13.3% on an average whereas in control it was 86.6%. The intimate association between S. paraphysata and Puccina substriata was proved by ultra microscopic structures in this investigation.
FOXO mediates organismal hypoxia tolerance by regulating NF-κB in Drosophila

Exposure of tissues and organs to low oxygen (hypoxia) occurs in both physiological and pathological conditions in animals. Under these conditions, organisms have to adapt their physiology to ensure proper functioning and survival. Here we define a role for the transcription factor FOXO as a mediator of hypoxia tolerance in Drosophila. We find that upon hypoxia exposure, FOXO transcriptional activity is rapidly induced in both larvae and adults. Moreover, we see that foxo mutant animals show misregulated glucose metabolism in low oxygen and subsequently exhibit reduced hypoxia survival. We identify the innate immune transcription factor, NF-KappaB/Relish, as a key FOXO target in the control of hypoxia tolerance. We find that expression of Relish and its target genes are increase in a FOXO-dependent manner in hypoxia, and that relish mutant animals show reduced survival in hypoxia. Together, these data indicate that FOXO is a hypoxia inducible factor that mediates tolerance to low oxygen by inducing immune-like responses.
Novel morphological structures in frogs: vocal sac diversity and evolution in Microhylidae (Amphibia: Anura)

Vocal sacs are present in most species of frogs and are the product of the interaction of three elements: the gular skin, the superficial submandibular musculature and an internal mucosa derived from the buccal floor. In this paper, we surveyed the structural diversity in the vocal sac of microhylids and related families in 109 exemplar species, including 11 of the 13 currently recognized subfamilies. We defined five characters related to anatomical and histological properties of the m. interhyoideus, as well as the relationship of this muscle and the vocal sac internal mucosa. We describe a vocal sac configuration characterized by highly abundant elastic fibres in association with muscle fibres, a very unusual structure in animal tissues. We discuss the evolution of novel structures in a phylogenetic context and identify new synapomorphies for Microhylidae and internal clades. Furthermore, we comment on the functional implications that these features have in vocal sac inflation.
Tracking Iris Surface Deformation Using Elastic Graph Matching

We propose a method to track the iris surface deformation in image sequences captured by a special infrared illuminated high-speed camera using elastic graph matching. A circular grid elastic graph (iris graph) to track the iris radial and circular movement of pupillary activity caused by varying lighting conditions is introduced. We compare the phase similarity with the magnitude similarity for tracking iris features and we also relate the determination of the weight of topography preservation in the similarity cost function to the amount of movement of the object being tracked. The algorithm is tested with a series of synthetic iris images and a series of real iris images. We show that the deformation of the iris surface area during the constriction of the pupil operates mainly in the middle and the peripheral parts of the iris and that this deformation is non linear.
Client Application for KBN (Personal IPTV Programme Guide)

A knowledge based network (KBN) is a content based network with utilising ontology comparison and bag operations. Compared with traditional centralized client-server web applications, KBN’s adopt the Publish-Subscribe model for communication between loosely coupled producers and consumers. Rather than using full message types, KBNs match the contents of messages from producers and the attribute description from subscribers so that the message will only be delivered to interested parties. The above features, loosed-coupling and event filtering, allow for great scalability in highly distributed systems. Based on Dominik Roblek’s work in his M.Sc in Computer Science (Networks and Distributed Systems) project last year, ontology comparison and a bag comparator matching algorithm have been implemented to support semantic distribution within the KBN. This project presents an implementation of a Personal IPTV Programme Guide, which applies ontology comparison and the bag comparator matching algorithm to a realistic client application for the KBN. Because it is the first time for KBN to face public users, this project focuses on the usability of such a client application. The design emphases the balance between the KBN required knowledge level and user knowledge background, including ontology concepts and using of operators. A usability test is also provided.
Unique and Overlapping Actions of Type I and III IFNs in Influenza A Virus Infection and Implications for Therapy

Influenza A virus (IAV) poses a significant public health burden. Severe disease is characterised by infected lung airway epithelial cells (AECs), inflammation and tissue damage. However, disease severity differs between individuals and this cannot be entirely explained by inter-individual differences in pre-existing immunity or comorbidities. Host-specific, genetically determined factors must contribute to susceptibility. Type I interferon (IFNαβ) is known to have antiviral function in vitro, but its role in restricting IAV infection in vivo is controversial. We demonstrate that responsiveness to IFNαβ signalling is a host-specific determinant with protective or pathogenic potential which determines the severity of IAV-induced disease. IAV infected 129, CBA/J and DBA mouse strains showed dramatically increased mortality and lung damage, yet higher levels of pulmonary IFNαβ, compared to C57BL/6 or BALB/C mice. Ablation of IFNαβR signalling in 129 mice markedly reduced mortality, levels of proinflammatory cytokines, inflammatory cell recruitment and AEC apoptosis. Susceptibility to IAV-induced disease is influenced by the number of functional alleles for the IFNαβR subunit, IFNAR1, within the genome. IFNAR1+/-(129) mice were less susceptible than wild type 129s yet more so than IFNαβR-/-(129) mice. Conversely, triplication of a section of murine chromosome 16 that includes IFNAR1 in C57BL/6 mice enhanced IFNα response to IAV infection and downstream immunopathology. Finally, IFNα therapy of infected B6. A2G-Mxl mice reduced IAV titers, yet increased secretion of proinflammatory cytokines, innate cell recruitment and AEC apoptosis in the lung, due to the potent immunostimulatory capability to IFNα. In contrast, treatment with IFNλ, whose receptor is largely restricted to AECs, promoted IAV control without exacerbating IAV-induced inflammation. Thus, by manipulating the IFNαβ signal in various ways, we demonstrate that excessive IFNαβ in IAV infection can increase AEC death and enhance proinflammatory responses that ultimately increase disease severity. Our findings have important implications for prediction and treatment of severe influenza.
Probeless Illumination Estimation for Outdoor Augmented Reality

Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain ? You may freely distribute the URL identifying the publication in the public portal ? Take down policy If you believe that this document breaches copyright please contact us at vbn@aub.aau. dk providing details, and we will remove access to the work immediately and investigate your claim.
Adesso : ambiente para desenvolvimento de software cientifico

Este documento descreve a concepcao e implementacao do Adesso, um ambiente computacional de suporte ao desenvolvimento de aplicacoes cientificas. O Adesso explora o modelo de programacao baseado em componentes reutilizaveis para fornecer suporte ao desenvolvimento de componentes e sua integracao a diversas plataformas de programacao cientifica. O Adesso baseia-se em uma base de dados de componentes representada em XML (toolboxes) e em um conjunto de ferramentas de transformacao para geracao automatica de codigo, documentacao e empacotamento dos componentes. O processo de transformacao e controlado por folhas de estilos definidas em uma linguagem que mescla elementos estruturais da linguagem XSLT com procedimentos e comandos da linguagem Tcl. Este trabalho apresenta a linguagem de transformacao de documentos XML implementado para o Adesso. Foram implementadas, na linguagem do processador de estilos do Adesso, diversas folhas de estilo que possibitam a transformacao de uma toolbox em pacotes de componentes para utilizacao nas plataformas MATLAB e Tcl/Tk. Sao gerados automaticamente, para cada toolbox, a documentacao em HTML e PDF, codigo fonte das interfaces e regras de construcao (Makefiles). Abstract
Identifying Potentially Avoidable Emergency Department Visits of Long-Term Care Hospital Residents in Korea: A Multicenter Retrospective Cohort Study

The aims of this study were to investigate the reasons of transfers from long-term care hospitals (LTCHs) to emergency departments (EDs) of university hospitals in geriatric patients and to categorize the avoidable causes of these transfers. This retrospective multicenter study involved patients aged 65 years and older who were transferred from LTCHs to 5 EDs of university hospitals located in the metropolitan area of South Korea between January 2017 and December 2017. The expert panel reviewed and categorized the reason of transfers as avoidable or not. Moreover, we also investigated the number of patients with do-not-resuscitate (DNR) documents and the date these DNR documents were written. A total of 255,543 patients visited 5 EDs during the study period. Of these, 1,131 patients were from LTCHs. The number of potentially avoidable transfers was 168/1,131 (14.9%). The most common reason of avoidable transfers was noncritical diagnoses that could be assessed and managed in LTCHs (57.1%). There were 162 patients with DNR orders; of these, 12 had approved the DNR order before transfer. In conclusion, in Korea, potentially avoidable transfers could be reduced by managing noncritical diseases in LTCH and preparing advance care directives, including DNR orders, during admission to LTCH.
PUBLISHING PATIENT DATA SET SECURELY THROUGH DIFFERENTIAL PRIVACY VIA WAVELET

Every organization stores a required data in the digital form. This data needs to be published due to the mutual benefits or due to the government rule. This data also has significant research value. While publishing a data in its original form, there is loss of privacy of the individual record. To avoid this, we can use e -differential privacy. Dwork provides a simplest way to achieve this. But it does not work well with range count query. In this paper we have used Privelet+ method using array based implementation for wavelet transforms. This method shows better result than the Dwork method for the range count queries.
The Fast Computation Methods for Extreme Learning Machine

The extreme learning machine (ELM) that is proposed by Huang is designed based on single-hidden layer feedforward neural networks (SLFNs), which can randomly choose the parameters of hidden nodes and the output weights gotten analytically. So it can get the solution fastly. However, the learning time of ELM is mainly spent on calculating the Moore-Penrose generalized inverse matrices of the hidden layer output matrix. This paper mainly focuses on the effective computation of the Moore-Penrose generalized inverse matrices for ELM. Moreover, several methods are proposed, which are tensor product matrix ELM (TPM-ELM), Geninv ELM Numerical experiments show that both Geninv-ELM and TPM-ELM are faster than other kinds of ELM and can reach comparable generalization performance.
Isotopic niches of Mesopelagic fish on the southern Kerguelen Axis: variation and overlap

Mesopelagic fish are an important but poorly understood mid-trophic component of Southern Ocean food webs. This study aimed to investigate the trophic role of mesopelagic fish in the southern Kerguelen Plateau region. We used carbon and nitrogen stable isotopes to quantify the isotopic niches of key mesopelagic fish species (six from the family Myctophidae, and one from Bathylagidae), to investigate variation along the δ15N and δ13C axes between species, and to examine the relationship between fish body length and δ15N within species and across the community. The assemblage of key mesopelagic fish occupied similar isotopic niche space with overlap among species in both δ13C and δ15N values. However, there were areas of non-overlapping isotopic niche space between species providing evidence of niche partitioning most likely driven by interspecific differences in diet and body size. At the community level, fish standard length was positively correlated with δ15N suggesting size-related differences in diet between species. However, within-species relationships between fish standard length and δ15N were weak. Overall, the findings from this study suggest that while there is a degree of trophic diversity among the taxa examined, this assemblage comprises a single energy pathway, which is informative for overall understanding of food-web architecture as well as development of trophically orientated ecosystem model for this region.
Changes in Mannitol Content, Regulation of Genes Involved in Mannitol Metabolism, and the Protective Effect of Mannitol on Volvariella volvacea at Low Temperature

The mechanism of autolysis of Volvariella volvacea (V. volvacea) at low temperature has not been fully explained. As mannitol is among the most important osmotic adjustment substances in fungal resistance, this study sampled mycelia of strains V23 and VH3 treated at 0°C for 0, 2, 4, 8, and 10 h to analyze changes in intracellular mannitol content by high-performance anion chromatography with pulsed amperometric detection (HAPEC-PAD). Reverse transcription quantitative PCR (RT-qPCR) analysis was applied to assess differences in the transcript levels of genes associated with mannitol metabolism under low-temperature stress. A mannitol solution was added to cultures of V. volvacea fruiting bodies, and effects on the hypothermic resistance of these organs were explored by evaluating variations in sensory properties during cryogenic storage after harvest. The results suggested that in the initial stage of low-temperature treatment, intracellular mannitol was largely catabolized as an energy storage material and the expression of genes encoding enzymes involved in synthetic reactions was inhibited. However, low-temperature resistance was induced with further treatment, with activation of mannitol synthesis and inhibition of degradation; the cells accumulated mannitol, leading to osmoregulation. No significant elongation of V. volvacea fruiting bodies during storage at 4°C was observed, and these organs tended to shrink and collapse. The sensory quality of mannitol-treated fruiting bodies was much better than that of control fruiting bodies. Application of a mannitol solution at the cultivation stage of V. volvacea somewhat improved the low-temperature resistance of the fruiting bodies, verifying the correlation between mannitol and resistance to this stress in V. volvacea. The results of this study lay a foundation for a deeper understanding of the autolysis mechanism of V. volvacea, providing technical support for increasing the cryopreservation time of this species and extending the postharvest shelf life of its fruiting bodies. In addition, the mechanism underlying the low-temperature tolerance of the VH3 strain should be further explained at the molecular level.
Effect of Cold Stress on Milk Yield, Physiological and Hemato-biochemical Profile of Cross Bred Dairy Cattle

The present study was performed to evaluate haematological and biochemical parameters with response to milk production of dairy cattle at low temperature. Ambient temperature and relative humidity were recorded and the temperature-humidity index (THI) was calculated as indicator of thermal comfort zone for cattle. Lactating crossbred dairy cattle were selected for this study. A significant increase in RBC, Hb, and PCVWBC was recorded in winter. Glucose and aspartate aminotransferase (AST), albumin level showed a significant increase in winter. Milk production level was decrease due to cold stress. These results provided an insight into the haematological and biochemical responses of Jersey crossbred cows to different environmental conditions. Hence, this study will be helpful for the better dairy cattle management in winter seasons for higher production at the cold arid high altitude region