Miracle-dz/UniFAR-FTUs huggingface dataset:
Clinical significance of Myb protein and downstream target genes in salivary adenoid cystic carcinoma

Adenoid cystic carcinoma (ACC), the second most frequent malignancy of the major and minor salivary glands, comprise of approximately 15-23% of all carcinomas at these locations. ACC is uniquely formed of dual epithelial and myoepithelial cells that give rise to different phenotypic patterns. We hypothesize that the dual myoepithelial/ epithelial composition of ACCs underlie their biological heterogeneity and may impact on their therapeutic management. A recurrent reciprocal translocation of t(6;9)(q22-23;p23-24) resulting in fusion gene partners comprising MYB gene the transcription factor NFIB has been reported in ACC of breast, salivary, lachrymal and ceruminal glands. In fusion positive and a subset of fusion negative ACCs, high expression of the transcript Myb was found.However, the role of Myb protein expression and the potential effect on the downstream targets have not been investigated. To investigate the biological and prognostic significance of use of elevated levels of Myb and its downstream target genes (c-kit, cox-2, bcl-2), we analyzed, by immunohistochemistry, the protein expression of these genes in 156 ACCs. We have found that 55% of ACCs have increased Myb expression mainly confined to myoepithelial cells. We validated Myb expression on a large cohort of ACCs (156 patients). Although no significant effects of the individual Myb and downstream targets c-kit, bcl-2 and cox-2 on survival was noticed, the combinations survival curve for Myb+/c-kit+/cox-2+ showed better survival than combination Myb-/c-kit+/cox-2+. Myb may serve as a new target for the management of this disease, and future therapeutic trials of these tumors may be better based on biomarker stratification and the cellular composition of these tumors.
Nutritional support in obstetrics and gynecology.

3 areas of in which nutritional support for the patient has profound implications - pregnancy lactation and sex hormone therapy - are reviewed with emphasis on altered nutrient needs. Nutrition as an influence on the course and outcome of pregnancy is reviewed with regard to energy requirements weight gain and relationship to birth weight. Nutritional requirements for protein iron folate calcium and vitamin D vitamin B(6) and sodium are presented and clinical implications discussed. During lactation protein and calcium content appear to be relatively independent of maternal nutritional status. Other nutrients such as amino acid fatty acid and water soluble vitamin composition vary with maternal intake. Nutritional support during hormonal therapy includes an increased need for vitamin B(6) and folate. Other nutrients which may require greater intake in those taking oral contraceptives are also reviewed.
Takagi Sugeno fuzzy observer based Direct Rotor Field Oriented Control of induction machine

This paper proposes a Takagi Sugeno (TS) fuzzy field observer in a rotor-flux-oriented reference frame. The proposed approach dealt with the conventional Direct Rotor Field Oriented Control (DRFOC) in a high speed application. The main aims are to provide new methods to observe the flux in same reference frame as the controller. In the initial development, we propose a TS model for the induction machine. Later, a TS fuzzy full order observer is described. Then a stability and a D-stability analysis are presented to proof in the first time, the stability and to garret in the second time, some dynamic behaviour for the observer through the Lyapunov theory. The proposed results are based on LMIs terms (Linear Matrix Inequalities) which can be solved easily using Matlab tools. A numerical example is provided to show the effectiveness of the proposed results.
The LIM protein, CRP1, is a smooth muscle marker

LIM domains are double zinc‐finger motifs found in many proteins that play central roles in cell differentiation. Members of the cysteine‐rich protein (CRP) family display two LIM domains and are implicated in muscle development. Here we describe the characterization of one member of this family, CRP1, in the mouse. We have isolated and sequenced murine cDNAs that encode CRP1. We have determined by Northern analysis and in situ hybridization that CRP1 expression is developmentally regulated in the embryonic mouse and displays organ specific regulation in adults. The gene encoding CRP1 is expressed in the smooth muscle cells (SMCs) of the dorsal aorta at E9.5, thus illustrating that CRP1 is an early marker for SMC differentiation at that site. As development proceeds, CRP1 transcripts are observed throughout the SMC lineage, with minimal, transient expression detected in skeletal and cardiac muscle. Interestingly, although several markers of mature smooth muscle are already expressed, CRP1 expression in the bladder is not upregulated until the onset of bladder expansion at embryonic day 16.5, at which time its expression becomes very prominent. CRP1 expression persists into adulthood with prominent expression observed in both vascular and visceral smooth muscle. The results reported here define CRP1 as a general marker of smooth muscle lineages. Dev Dyn 1999;214:229–238. © 1999 Wiley‐Liss, Inc.
On Necessity and Feasibility of Establishing Rural Logistics System in China

This paper introduces contemporary logistics into the management system of our rural agricultural products and proposes establish our rural logistics.According to the fact of our country and combining some success experience of overseas countries logistics,it related the feasibility and the need to establish our rural logistics in WTO,agricultural structure,the rural service and cost check,etc.
Quantitative Analysis of CBP- and P300-Induced Histone Acetylations In Vivo Using Native Chromatin

ABSTRACT In vivo, histone tails are involved in numerous interactions, including those with DNA, adjacent histones, and other, nonhistone proteins. The amino termini are also the substrates for a number of enzymes, including histone acetyltransferases (HATs), histone deacetylases, and histone methyltransferases. Traditional biochemical approaches defining the substrate specificity profiles of HATs have been performed using purified histone tails, recombinant histones, or purified mononucleosomes as substrates. It is clear that the in vivo presentation of the substrate cannot be accurately represented by using these in vitro approaches. Because of the difficulty in translating in vitro results into in vivo situations, we developed a novel single-cell HAT assay that provides quantitative measurements of endogenous HAT activity. The HAT assay is performed under in vivo conditions by using the native chromatin structure as the physiological substrate. The assay combines the spatial resolving power of laser scanning confocal microscopy with simple statistical analyses to characterize CREB binding protein (CBP)- and P300-induced changes in global histone acetylation levels at specific lysine residues. Here we show that CBP and P300 exhibit unique substrate specificity profiles, consistent with the developmental and functional differences between the two HATs.
Customer frustration in loyalty programs

Purpose – This article aims to examine the negative effects of loyalty programs from the perspective of frustration theory. It seeks to develop a model of customer frustration on the basis of frustration theory and an exploratory qualitative study.Design/methodology/approach – First, frustration is defined as a special form of dissatisfaction and a general model of frustration in business relationships is developed by evaluating the literature on frustration theory. Second, an explorative and qualitative focus group study among participants of a loyalty program for frequent travelers is conducted. A multi‐level iterative content analysis of the participants' statements reveals the existence of different categories of frustration incidents. Third, the findings of the study are used to develop a system of propositions that generate a specific model of customer frustration in loyalty programs.Findings – Seven categories of frustration incidents that were triggered by the loyalty program and lead to frustrati.
Performance Analysis of a Propagation Territory-based Routing in MANETs with Obstacles

This study considers constructing an efficient routing protocol in MANETs with obstacles. We take advantage of the location information provided by Global Position System (GPS) and propose a novel greedy propagation territory-based routing mechanism to establish the routing path between source and destination nodes. The mechanism consists of four phases and each phase defines a propagation territory and routing strategy to make a locally optimal decision to forward packets. For each mobile wireless node, including source and relay nodes, the mechanism selects a node in their propagation territory that is closest to the destination as a next hop relay node to deliver the packets until the destination is reached. In addition, when source and relay nodes moves closer to obstacles, the signal interferences by obstacles are considered. If the radio signal of a node is disturbed by an obstacle, the neighboring node with the lowest signal interference in its propagation territory is substituted as a next hop relay node. The simulation results showed that the proposed routing mechanism demonstrates a better performance in terms of the average packet delivery ratio than those of the ad-hoc on demand distance vector and greedy perimeter stateless routing schemes when the nodes move at the high-moving speed scenario. In the low-speed scenario, the average delay hop count of the proposed routing mechanism is less than those of the other routing schemes.
The Sony Ericsson WTA Tour 10 year age eligibility and professional development review

Concerns have long existed over the participation of adolescent athletes in professional sports. In 2004, the Sony Ericsson WTA Tour (WTA Tour) commissioned a Professional Development Advisory Panel (PDAP) to evaluate the WTA Tour’s age eligibility rule (AER) and professional development programmes (PDPs) for female tennis players since their inception in 1995. More than 75% of the 628 respondents supported the principles of the AER, and 90% indicated a need for PDPs. Statistical analysis of WTA Tour players’ careers found that premature retirements (players leaving the Tour at or before age 21) decreased significantly from 7% before the AER to less than 1% afterward, and median career length increased by 43%. The PDAP recommends that the WTA Tour continues a phased-in, developmentally appropriate AER, enhances the PDPs, and works with other sport governing bodies to coordinate rules and programmes at earlier ages to aid the transition of adolescents into adult sports.
Relationship marketing and the consumer

ConclusionCollectively, the responses of the individuals interviewed reveal that there is a multitude of reasons for participating in a marketing relationship. Although many of the reasons are cognitive in nature, many others are affective. This affective “dimension” of relationship marketing remains unexplored but would seem to offer great promise for explaining the long-term nature of a firm-consumer relationship. Moreover, a liberal interpretation of the collective responses of the interviewed individuals suggests an alternative fundamental axiom of relationship marketing consistent with the definition of Angeles (1992):Consumers enter into a marketing relationship because they expect to receive positive value from their participation.Acceptance of this axiom would seem to suggest a conceptualization and research agenda much broader and potentially more productive than that associated with the axiom currently being offered.Sheth and Parvatiyar have raised a question that, despite its obviousness in retrospect, has yet to be addressed satisfactorily. Hopefully their article will prove to be the genesis of an answer. Although no one is likely to agree with all of their propositions, or even their speculation that relationship marketing will ultimately improve marketing productivity, Sheth and Parvatiyar have done the discipline a service by focusing attention on a neglected topic. Indeed, what they have effectively done is to challenge the discipline to debate what they believe to be the fundamental axiom of relationship marketing in consumer markets. Regardless of the outcome of this debate, marketing knowledge will have advanced.
SGO: management information system for strategic bidding in electrical markets

This paper describes SGO, a management information system for bidding in deregulated electricity markets, developed for the Spanish case. SGO has a client-server architecture and consists of a set of integrated cooperative and flexible software tools for assisting the users during the whole bidding process: resources identification, bids generation, market performance characterisation, bidding strategy analysis and optimisation, generation of markets results reports and automatic performance monitoring for suggesting on-line corrective actions.
Charge transport modeling in insulating polymers: from molecular to macroscopic scale

More than fifty years after the publication of the early work on conduction and dielectric breakdown of solids, we are still unable to describe quantitatively the electrical response of these materials. During this period of time, concepts derived from semiconductor physics have been transposed to the case of insulating solids, and among them, to polymers. Alternative descriptions have been proposed as well. In spite of this, there is still no agreement on how to describe charge transport and there is still some controversy as regards the applicability of semiconductors physics to the case of disordered insulating materials and in particular to polymers used in electrical engineering applications. The last twenty years have been marked by the publication of excellent review papers summarizing the physical concepts available to describe charge transport. Enormous steps forward have been achieved as regards computing facilities and our ability to spatially map the space charge, quantitatively, inside dielectric materials. We consider these two factors as fundamental in providing possibilities for developing sound models of charge transport, by using the basis of fundamental knowledge that has been accumulated in the previous years, and by coupling up-to-date techniques in experiments and in simulation. In this paper, which is not a review of either the published work on modeling or of new concepts in dielectric physics, we emphasize recent progress in the field of atomistic and macroscopic modeling and we discuss challenges based on such approaches that, we think, constitute a direction for future research.
Human Cytomegalovirus Latency-Associated Protein pORF94 Is Dispensable for Productive and Latent Infection

ABSTRACT Human cytomegalovirus latency in bone marrow-derived myeloid progenitors is characterized by the presence of latency-associated transcripts encoded in the ie1/ie2 region of the viral genome. To assess the role of ORF94 (UL126a), a conserved open reading frame on these transcripts, a recombinant virus (RC2710) unable to express this gene was constructed. This virus replicated at wild-type levels and expressed productive as well as latency-associatedie1/ie2 region transcripts. During latency in granulocyte-macrophage progenitors, RC2710 DNA was detected at levels indistinguishable from wild-type virus, latent-phase transcription was present, and RC2710 reactivated when latently infected cells were cocultured with permissive fibroblasts. These data suggest pORF94 is not required for either productive or latent infection as assayed in cultured cells despite being the only known nuclear latency-associated protein.
Structure‐based modeling of the ligand binding domain of the human cell surface receptor CD23 and comparison of two independently derived molecular models

CD23, a type II membrane receptor protein, recognizes four different ligands via its extracellular C‐type lectin domain: immunoglobulin E (IgE), CD21, and the β2‐integrins CD11b and CD11c. CD23 specifically interacts in a calcium‐dependent manner, “lectin‐like” with carbohydrate moieties expressed on CD21 and CD11b/c, but also “lectin‐unlike” with protein epitopes on IgE. As a first step in analyzing the multiple binding specificities associated with CD23 in more detail, we report a detailed molecular model of the lectin‐like domain of human CD23 (hCD23). The model was built based on information provided by X‐ray structures of mannose binding protein (MBP) and E‐selectin, both of which are members of the calcium‐dependent (C‐type) lectin superfamily. Sequence‐structure comparisons suggest that hCD23 is structurally more similar to MBP than to E‐selectin. The hCD23 model is compared to an independently derived model. Although the CD23‐carbohydrate and CD23‐protein interactions are both calcium dependent, analysis of the model suggests the presence of distinct binding sites for these ligands.
Robustness of sub-70 nm dynamic circuits: analytical techniques and scaling trends

We present an accurate (to within 3%, across two process generations), three parameter, closed form, time-domain technique for evaluating the noise response of domino circuits. We evaluate the robustness of scaled topologies to show that conventional domino circuits will cease to be useful around the 70 nm generation. The paper concludes with possible device/circuit approaches to extend domino circuit usefulness.
Mutation position and type of substitution in the beta-subunit of the RNA polymerase influence in-vitro activity of rifamycins in rifampicin-resistant Mycobacterium tuberculosis.

Quantitative susceptibility testing for rifampicin, rifabutin and rifapentine of 36 Mycobacterium tuberculosis isolates with known sequences for the gene encoding for the RNA polymerase β-subunit (rpoB) revealed that both mutation position and type of amino acid substitution influence the in-vitro activity of rifamycins in rifampicin-resistant strains
Fast Aggressive Trading

We subdivide trades on the London Stock Exchange according to their reaction times. We classify an aggressive order as 'fast' if it executes against a standing limit order that is less than 50 milliseconds old. We show that fast trades are associated with smaller execution costs than slow trades. However, fast trades lead to virtually no permanent price impact while slow trades have the usual positive long run price impacts. We find little evidence that fast traders manipulate counter-parties into trading with them at improved prices. Overall, the subset of fast traders we isolate are fairly innocuous. This suggests that regulating trading speed in order to curb issues thought to be associated with high-frequency trading may be at best a very blunt tool and, at worst, may impose significant costs on those who use low-latency systems to execute efficiently.
4-HPR-mediated leukemia cell cytotoxicity is triggered by ceramide-induced mitochondrial oxidative stress and is regulated downstream by Bcl-2

We have previously reported that, in leukemia cells, the cytotoxicity of the anticancer agent N-(4-hydroxyphenyl)retinamide (4-HPR) is mediated by mitochondria-derived reactive oxygen species (ROS) and cardiolipin peroxidation. Here, we have analyzed at greater depth the 4-HPR-triggered molecular events, demonstrating that 4-HPR induces an early (15 min) increase in ceramide levels by sphingomyelin hydrolysis and later (from 1 h) by de novo synthesis. Using specific inhibitors of both pathways, we demonstrate that ceramide accumulation is responsible for early ROS generation, which act as apoptotic signalling intermediates leading to conformational activation of Bak and Bax, loss of mitochondrial membrane potential (ΔΨm), mitochondrial membrane permeabilization (MMP) and cell death. Enforced expression of Bcl-2 has no effect on 4-HPR-induced oxidative stress, but notably prevents mitochondrial alterations and apoptosis, indicating that Bcl-2 functions by regulating events downstream of ROS generation. In conclusion, our study delineates for the fist time the sequence and timing of the principal events induced by 4-HPR in leukemia cells and points to the potential use of modulators of ceramide metabolism as enhancers in 4-HPR-based therapies.
Evidence for Unequal Misses in Oxygen Flash Yield Sequence in Photosynthesis

The numerical analysis of the oxygen flash yield Yn sequences, alone, does not allow to choose between two models: equal S state misses with non negligible double hits or unequal misses with nearly no double hits. Nevertheless, the comparison of the sequences in different conditions shows that the equal miss model is unrealistic: in very different experimental conditions (non saturating flash, different batch of Chlorella or chloroplasts), a parallel variation of the homogeneous miss and double hit factors is observed. This correlation seems strange within the equal miss model: misses come from incomplete reaction (i.e. for exemple insufficient light) and double hits i.e. double advancement come, in principle, from excessive light or too long flash; for these reasons, opposite variation of misses and double hits as a function of light intensity are expected. Within the equal miss model the inverse is exactly observed: at low flash light intensity (11%) which increases the misses, 16% of double hits are needed, which is quite unrealistic. In contrast, the unequal miss model explains such result quite naturally by a m athem atical property: any theoretical sequence with only a unique S state miss and no double hit can be fitted with homogeneous misses and double hits, which increase in parallel as a function of the damping. Evidence for unequal misses in oxygen flash yield sequence is provided by the heterogeneous properties of the light saturation curves (M. J. Delrieu, Biochim. Biophys. Acta 592, 478-494 (1980)). At high flash intensity, all, excepted the transition S'2 → S3, are saturated; the transition S'2-→S3 is far from saturation and its very large saturating light intensity is actually not known. A comparative study, in the same chloroplast batch, of the oxygen yield patterns with attenuated flashes and of the experimental saturation curves of S states shows that only photochemical misses (due to non saturation) exist. At high intensity, there is only a unique miss for the transition S2→S3 i.e. the probability for this transition is low. A model involving a second acceptor could explain the slow increase of transition probability of S'2→S3 at high flash intensity
Consumer File Sharing of Motion Pictures

Illegal consumer file sharing of motion pictures is considered a major threat to the movie industry. Whereas industry advocates and some scholars postulate a cannibalistic effect on commercial forms of movie consumption, other researchers deny this effect, though sound evidence is lacking on both sides. Drawing on extant research and utility theory, the authors present hypotheses on the consequences and determinants of consumer file sharing and test them with data from a controlled longitudinal panel study of German consumers. The data contain information on the consumers' intentions toward and actual behavior in relation to the consumption of 25 new motion pictures, allowing the authors to study more than 10,000 individual file-sharing opportunities. The authors test the effect of file sharing on commercial movie consumption using a series of ReLogit regression analyses and apply partial least squares structural equation modeling to identify the determinants of consumer file sharing. They find evidence of substantial cannibalization of theater visits, DVD rentals, and DVD purchases responsible for annual revenue losses of $300 million in Germany. Five categories of file-sharing behavior drive file sharing and have a significant impact on how consumers obtain and watch illegal movie copies.