Miracle-dz/UniFAR-FTUs huggingface dataset:
Développement d'un biosenseur fluorescent d'un mutant de p53 sujet à l'agrégation dans les cancers

P53 est un suppresseur de tumeur qui joue un role cle dans la regulation de la transcription, la reparation de l'ADN, l'instabilite genetique, la senescence, la regulation du cycle cellulaire et l'apoptose. Cette proteine, normalement nucleaire, se lie a l’ADN et regule la transactivation. Cependant, elle est souvent mutee dans les tumeurs humaines, entrainant une inactivation fonctionnelle et une predisposition au cancer. Les mutants p53 se peuvent-etre de deux categories des mutants de « contact » ou « conformationnel ». Ces derniers entrainant des changements de conformation pouvant induire une agregation de la proteine de type amyloide. Des etudes recentes ont montre que l’agregation de p53 pouvait etre efficacement reversee, retablissant ainsi la fonction de p53 chez la souris. Les approches de diagnostic actuelles basees sur le sequencage genetique permettent d'identifier le statut mutationnel de p53, mais ne renseignent pas sur son statut conformationnel. Le but de ma these est de developper un biosenseur peptidique fluorescent qui reconnait et rend compte des mutants conformationnels de p53 exprimes dans des cancers humains. Nous avons caracterise et optimise la reponse de ce biosenseur par conjugaison avec differentes sondes sensibles a l'environnement. Nous avons egalement etudie leur capacite a signaler de maniere selective le mutant R248Q de p53 in vitro a l’aide de formes recombinantes de proteines p53 de type sauvage et mutantes, ainsi que de lysats de lignees cellulaires de cancer du poumon exprimant p53 de type sauvage (A549), le mutant R248Q (PC9) ou p53 - / - (H1299). Apres avoir etabli les conditions de travail optimales et les limites du biocapteur in vitro, nous avons applique ce biosenseur a l’imagerie de cellules vivantes par microscopie a fluorescence, apres avoir procede a l’internalisation cellulaire facilitee par un peptide vecteur (ou CPP : Cell Penetrating Peptide) afin d’etablir son potentiel pour de futures applications de diagnostic et therapeutiques. Nous avons de plus verifie la reponse du biosenseur a l'expression induite de mutants conformationnels de p53, ainsi qu'a sa regulation negative, a la fois in vitro et dans des cellules vivantes.
Effect Of Topical Tinospora Cordifolia On Healing Of Burn Wounds In Wistar Rats

Objective: To investigate the effect of tinospora cordifolia (TC) on the healing of burn wounds in wistar rats and compare it with control and silver sulfadiazine treated groups. Materials and Methods: Partial thickness burn wounds were made on three groups of rats (n=6 in each group) at the nape of neck under ether anesthesia. The Burn wounds were treated topically with petroleum base, 1% silver sulfadiazine and 2% TC, respectively once daily till complete healing. The wound contraction rate and epithelialization time were observed. Data was analysed by one way ANOVA and Tukeys multiple post hoc procedures. Results: The mean epithelialization time was significantly (P<0.0004*) decreased in TC treated group compared to control and silver sulfadiazine groups. Percentage of wound contraction was significantly more on 4 (P<0.0003*), 8th (P<0.0019*), 12th (P<0.0060*) and 16th day (P<0.0016*) in TC treated group in comparison to the control. Conclusion: Topical TC showed healing potential on burn wounds in wistar rats.
Intergenerational Coresidence and Depressive Symptoms of Rural and Urban Older Adults in China

Objectives: This study aimed to examine whether gender and marital status of coresiding adult children are associated with depressive symptoms of Chinese older adults. Methods: Using data from the China Health and Retirement Longitudinal Study, linear regression analysis was conducted to identify longitudinal associations of intergenerational coresidence with depressive symptoms in rural and urban older Chinese. Results: Both rural and urban older adults living with unmarried sons had significantly higher depressive symptoms at four-year follow-up than those who did not live with children. Living with married sons was significantly associated with higher levels of depressive symptoms at four-year follow-up among rural elders only. Discussion: This study sheds light on the heterogeneity in the relationship between intergenerational coresidence and Chinese older adults’ psychological well-being by the gender and marital status of coresiding children. Further research is needed to understand the complex and dynamic household structures and health outcomes in later life.
Enhancing Business Performance of Pakistani Manufacturing Firms via Strategic Agility in the Industry 4.0 Era

Manufacturing plays a substantial role in the economic development of any country because of its multiplier impact on the growth of value addition. Currently, industry 4.0 requires manufacturers to deliver highly customized products without compromising on quality at a reduced life cycle. The objective of this study was to find out a solution for the optimum operation of manufacturing firms. By applying resource-based view, dynamic capability, and effectuation theory, this study has proposed an integrated framework of the organizational network, entrepreneurial bricolage, strategic agility and business performance in the context of the industry 4.0. Moreover, the positive effect of the organizational networks on the strategic agility ultimately improves the business performance of manufacturers. Furthermore, strategic agility is also claimed to play its role as mediator between organizational networks and business performance.
Civic Solidarity: Civicness and Willingness to Pay for the Poor in Vietnam

What makes people more willing to pay for fighting hunger and poverty, to give to charity, and to transfer a portion of their incomes to poorest regions? In this paper, I argue that the active participation in civic life that nurtures civicness in one’s mind reinforces the habits of the heart and instills a sense of solidarity and empathy toward less fortunate co-members of the community, thereby shaping preferences over social issues in favor of redistributive policies. By analyzing the 2017 Vietnam Provincial Governance and Public Administration Performance Index individual-level survey dataset, the paper shows that eight indicators of civicness of respondents, individually as well as collectively, prove strongly significant in explaining the variation in their willingness to pay for the poor in Vietnam.
Making up the mind: Intersubjectivity or introjectivity?

This article considers the term ‘post-Bionian’ and its context of ‘intersubjective field theory’ to be actually incompatible with Bion’s own ideas and their post-Kleinian context. The current usage of the terms seems, in the author’s view, to be an example of the sociology rather than the evolutionary Idea of psychoanalysis (in Bion’s own distinction).
Relationship between mean length of utterance and vocabulary in children with typical language development

ABSTRACT Purpose: to investigate the existence of a relationship between vocabulary and measures of mean length of utterance in children in their language development phase. Methods: the sample consisted of 72 children aged 2 to 4 years, 11 months and 29 days, 36 boys and 36 girls, with typical language development, evenly distributed into age groups, enrolled in kindergartens with the public school system, in Santa Maria, RS, Brazil. Videos of the spontaneous speech of each subject were made, and then, the analysis of the vocabulary and Mean Length of Utterance took place. Statistical analysis was performed using the Statistical Analysis System program, version 9.2 and Spearman correlation coefficient, with a significance level of p <0.05. Results: the influence of gender in the Mean Length of Utterance correlation and vocabulary was observed. There was a difference between the ages of 2 and 4 years. Conclusion: vocabulary development promotes mean length utterance, indicating positive correlation between gender and age range.
Driving Simulation as Virtual Reality Exposure Therapy to Rehabilitate Patients with Driving Fear After Traffic Accidents

Summary: Following a traffic accident, up to 30% of the involved persons suffer from stress related symptoms often coming along with enduring fear of driving. Virtual reality exposure therapy (VRET) offers major advantages for treating anxiety disorders, but with respect to fear of driving it has been hardly investigated so far. In the present study a driving simulator exposure treatment for patients with fear of driving after a traffic accident was developed and evaluated. The therapy followed a standardized manual of 13 sessions including anamnesis, medical ex-amination, two preparative psychotherapy sessions, five virtual reality exposure (VRE) sessions, a final behavioral avoidance test in real traffic with a driving instructor, a closing session, plus follow-up phone calls after six and twelve weeks. The exposure scenarios were individually tailored to the patients’ anxiety hierarchy. 14 patients were treated. Results indicate excellent treatment success. In the final behavioral avoidance test, all patients mastered driving tasks they had avoided before, 71% showed an adequate driving behavior as assessed by the driving instructor, 93% could maintain their treatment success until the second follow-up phone call. We conclude that VRET in a driving simulator is a highly promising tool to treat fear of driving. Major advantages are that traffic scenarios are highly controllable, safe and can be designed and presented to perfectly fit the individu-als' anxieties.
Peut-on se passer des progestatifs dans le traitement hormonal de la ménopause?

Le traitement hormonal de la ménopause (THM) est efficace contre les symptômes climatériques, il prévient l’ostéoporose et les fractures ostéoporotiques et améliore la trophicité. Les principaux risques du THM sont, en fonction des molécules et des voies d’administration, l’augmentation du risque thromboembolique, les accidents vasculaires cérébraux et l’augmentation du risque de cancer du sein. Ces faits sont acquis bien que différents si l’on tient compte du terrain et des formes d’administration du THM (1). Le risque le plus médiatisé concerne les risques carcinogènes et plus particulièrement l’augmentation de l’incidence du cancer du sein. Les THM sont nombreux et associent généralement les estrogènes et les progestatifs. Dès 1989, la publication de L. Bergkvist (2) attirait l’attention sur le rôle des progestatifs dans l’augmentation du risque de cancer du sein et depuis presque 20 ans, ce débat est récurrent et très polémique. Le rôle délétère des progestatifs a fait émettre l’idée que le traitement estrogénique pouvait être proposé seul et améliorerait la balance bénéfices-risques du THM. Cette idée, qui peut paraître saugrenue, a été écrite au chapitre de la discussion de la publication de la Million Women Study (MWS) [3] faisant état de 20 000 cancers du sein attribuables au THM et aux progestatifs, en particulier chez les femmes de 50 à 64 ans pour les dix dernières années. Peut-on, si le traitement estrogénique augmente ➤ beaucoup moins le risque de cancer du sein que le traitement estroprogestatif, accepter une augmentation du risque du cancer de l’endomètre ? Quels sont les éléments rationnels permettant ➤ une telle attitude ? Le médecin ne risque-t-il pas d’être poursuivi ➤ en cas de cancer de l’endomètre s’il a prescrit un traitement estrogénique seul ? Après avoir fait le bilan de la balance bénéfice-risque des progestatifs vis-à-vis des cancers, nous réfléchirons à la philosophie de cette attitude en soulignant le risque médico-légal qu’entraînerait la décision de ne pas prescrire de progestatif, car chacun sait qu’un cancer évité ne fait pas l’objet d’une prise de conscience alors qu’un cancer induit peut expliquer l’attitude revendicatrice de la personne touchée par cette complication. De très nombreuses études sont disponibles mais, par souci de rigueur scientifique et pour éviter la confusion inhérente aux résultats variables des études cas-témoins, nous insisterons sur les résultats des études d’intervention (en particulier la Women Health Initiative [WHI], l’étude de référence), sur les résultats des métaanalyses, sur la grande étude de cohorte : la MWS, et nous ferons appel à la grande étude de cohorte française E3N.
Coarse grained models of graphene and graphene oxide for use in aqueous solution

Obtaining stable aqueous dispersions of graphene-based materials is a major obstacle in the development and widespread use of graphene in nanotechnology. The efficacy of atomistic simulations in obtaining a molecular-level insight into aggregation and exfoliation of graphene/graphene oxide (GO) is hindered by length and time scale limitations. In this work, we developed coarse-grained (CG) models of graphene/GO sheets, compatible with the polarizable Martini water model, using molecular dynamics, iterative Boltzmann inversion and umbrella sampling simulations. The new CG models accurately reproduce graphene/GO–water radial distribution functions and sheet–sheet aggregation free energies for small graphene (−316 kJ mol−1) and GO (−108 kJ mol−1) reference sheets. Deprotonation of carboxylic acid functionalities stabilize the exfoliated state by electrostatic repulsion, providing they are present at sufficiently high surface concentration. The simulations also highlight the pivotal role played by entropy in controlling the propensity for aggregation or exfoliation. The CG models improve the computational efficiency of simulations by an order of magnitude and the framework presented is transferrable to sheets of different sizes and oxygen contents. They can now be used to provide fundamental physical insights into the stability of dispersions and controlled self-assembly, underpinning the computational design of graphene-containing nanomaterials.
Generating Situational Awareness of Pedestrian and Vehicular Movement in Urban Areas Using IoT Data Streams

Humans have continuously endeavored to enhance their sensory perception and awareness of the physical surroundings for the betterment of themselves as individuals as well as communities. Advancing this notion to the present day, the Internet of Things (IoT) provides a unique opportunity to attempt the same in an increasingly digital landscape. The prevalence and pervasiveness of IoT data streams gives us this ability to represent a situation with clarity and nuance, leading to a refined awareness of the environment. However, there are several challenges in managing the scale, velocity, and magnitude of IoT data streams, as well as the cohesive representation of these varied sources in a single frame of reference. In this article, we present a new algorithm, the deep growing self-organizing map (deep GSOM) algorithm that addresses these challenges. Deep GSOM incrementally generates a latent representation of situational awareness from high entropy to low entropy IoT data streams. It utilizes an implementation of the fuzzy integral to define a metric that can be moved across spatial and temporal situations to profile the density of congestion. We have also expanded deep GSOM into an IoT platform that can collate, aggregate, and process an entire network of IoT data streams. We demonstrate the workings of deep GSOM on the real-life scenario of profiling vehicular and pedestrian movement using IoT data streams of two highly urbanized cities. The results of these experiments confirm the validity and effectiveness of the proposed approach for generating situational awareness from multiple IoT data streams.
Association of Body Mass Index with Asthma Severity and Pulmonary Function among Asthmatic Children

Background Asthma is a chronic inflammatory disease in respiratory system and obesity is another inflammatory disease which incidence rate is increasing. Although, many studies have been conducted on severity of asthma and its relationship with obesity, but different results have been obtained. This study aimed to determine a relationship between asthma severity, Body Mass Index (BMI) and pulmonary function in Kurdistan province, Iran. Materials and Methods: In this cross sectional study 90 asthmatic patients referred to referral hospital in Kurdistan, North West of Iran, were selected by simple random method. BMI was calculated by dividing weight by height.Pulmonary Function Test (PFT) and bronchial-stimulation-test were used for confirmation and investigation of asthma severity. Data were analyzed using SPSS-15 and Chi-square and spearman correlation coefficient tests. Results: Relationship between BMI and severity of asthma (mild, medium and severe) was evaluated, there was a relationship and positive relationship between them (P 0.05), but there was a significant and inverse correlation between waist size and other parameters such as (FEV1, FVC and FEF25-75) (P<0.05). Furthermore, there was a significant and inverse correlation between spirometry indices and Waist-Hip ratio (WHR) (P<0.05) Conclusion It seems that attention to BMI in people with pulmonary diseases including asthma can help improve clinical and physiological conditions of the person.
Complex‐Shaped Cellulose Composites Made by Wet Densification of 3D Printed Scaffolds

Cellulose is an attractive material resource for the fabrication of sustainable functional products, but its processing into structures with complex architecture and high cellulose content remains challenging. Such limitation has prevented cellulose‐based synthetic materials from reaching the level of structural control and mechanical properties observed in their biological counterparts, such as wood and plant tissues. To address this issue, a simple approach is reported to manufacture complex‐shaped cellulose‐based composites, in which the shaping capabilities of 3D printing technologies are combined with a wet densification process that increases the concentration of cellulose in the final printed material. Densification is achieved by exchanging the liquid of the wet printed material with a poor solvent mixture that induces attractive interactions between cellulose particles. The effect of the solvent mixture on the final cellulose concentration is rationalized using solubility parameters that quantify the attractive interparticle interactions. Using X‐ray diffraction analysis and mechanical tests, 3D printed composites obtained through this process are shown to exhibit highly aligned microstructures and mechanical properties significantly higher than those obtained by earlier additively manufactured cellulose‐based materials. These features enable the fabrication of cellulose‐rich synthetic structures that more closely resemble the exquisite designs found in biological materials grown by plants in nature.
Soft tissue cephalometric analysis applied to Himachali ethnic population

Introduction: The modern society considers facial attractiveness as an important physical attribute. The great variance in soft tissue drape of the human face complicates accurate assessment of the soft tissue profile, and it is a known fact that facial features of different ethnic groups differ significantly. This study was undertaken to establish norms for Himachali ethnic population. Materials and Methods: The sample comprised lateral cephalograms taken in natural head position of 100 normal individuals (50 males, 50 females). The cephalograms were analyzed by Arnett soft tissue cephalometric analysis for orthodontic diagnosis and treatment planning. Student's t-test was used to compare the means of the two groups. Results: Statistically significant differences were found between Himachali males and females in certain key parameters. Males have thicker soft tissue structures and a more acute nasolabial angle than females. Males have longer faces and females have greater interlabial gap and maxillary incisor exposure. Males have more deep-set facial structures than females. Conclusions: Statistically significant differences were found between Himachali males and females in certain key parameters. Differences were also noted between other ethnic groups and Himachali faces.
Clinical Application of Liquid Chromatography Tandem Mass Spectrometry Using Dried Blood Spot as a More Rapid Method for Determination of Methylmalonic Acid, Propionylcarnitine, and Total Homocysteine

Methylmalonic acidemia (MMA) should be diagnosed in early infancy and receive appropriate management promptly after the diagnosis to prevent severe complications leading to death. At present, a newborn screening (NBS) method using tandem mass spectrometry (MS/MS) identifies suspected patients with MMA by elevated propionylcarnitine. In addition, a liquid chromatography tandem mass spectrometry (LC/MS/MS) method using dried blood spot is effective to detect some metabolites as a second-tier test, and reduces the false-positive rate in NBS. However, these tests were only used in screening, and not applied as an examination for evaluating treatment. Herein, we describe a 57-day-old girl with MMA under treatment with cobalamin who had elevated urinary methylmalonic acid levels. We applied the LC/MS/MS method with a separation column to evaluate her cobalamin responsiveness, and discovered an insufficient cobalamin dose earlier than would have been possible using other methods. Based on the current data, this method seems to be applicable for the follow-up of the treatment of MMA patients. However, this should be confirmed with more experience with a larger number of cases and a wider spectrum of disorders.
Znaczenie funduszy unijnych dla rozwoju przedsiębiorczości i innowacyjności regionu świętokrzyskiego. Przykład klastra Grono Targowe Kielce

Klastry pelnią istotną funkcje w rozwoju procesow przedsiebiorczości, opartych na innowacyjności. Jest to problematyka szeroko dyskutowana w literaturze zagranicznej, jednak wymaga w dalszym ciągu wnikliwych analiz na gruncie krajowym. Przedmiotem badan opisywanych w artykule bylo Grono Targowe Kielce – najwiekszy i najstarszy klaster w wojewodztwie świetokrzyskim. W pracy wykazano znaczenie wplywu funduszy strukturalnych pozyskiwanych przez czlonkow klastra na ukierunkowywanie charakteru rozwoju regionu świetokrzyskiego. Zanalizowano strukture finansowania projektow zrealizowanych przez podmioty gospodarcze nalezące do wybranego klastra, a takze wylącznie przez koordynatora zrzeszenia. Zakres czasowy objąl lata 2005–2013. Wykazano zwlaszcza realizacje, ktore dotyczyly wojewodztwa świetokrzyskiego, czyli zostaly przeznaczone bezpośrednio bądź pośrednio na jego rozwoj, z podzialem na poprawe przedsiebiorczości, innowacyjności i konkurencyjności. Wyniki badan pozwolily wskazac, iz środki pomocowe otrzymane przez klaster i jego czlonkow przyczynily sie do rozwoju wojewodztwa w rozpatrywanych plaszczyznach. Wiekszośc projektow zrealizowano podczas czlonkostwa w klastrze, a objely one glownie kapital ludzki. Klaster jako beneficjent bral udzial we wsparciu potencjalu dydaktycznego ośrodka akademickiego dla potrzeb rynku pracy, a takze w promocji gospodarczej i inwestycyjnej regionu.
The process of shaping partnerships for developing and implementing a membership system of walking exercise as a municipal health promotion program: IC-Walk provided by the Department of Health Promotion in M City

抄録 目的 : 本研究の目的は,健康づくり事業における会員制ウォーキングシステム事業化のためのパートナーシップ形成プロセ スを明らかにすることである. 方法 :M 市健康推進課の IC ウオーク事業(以下,本事業)担当保健師 2 名を対象とする半構造化インタビューおよび本事 業に関する記事を対象とした文書調査を行い,テキストデータの比較分析により,コードを抽出し,サブカテゴリー,カテ ゴリーに分類した. 得られたサブカテゴリーおよびカテゴリーを,その関連や時間的な経過を考慮して構造化し,パートナー シップ形成プロセスを分析した. 結果 : テキストデータの分析から 18 のコードが得られ,9 のサブカテゴリー(「首長」「議会」「協議会」「自主グループ」「他 課予算」「助成金」「民間」「庁舎内連携」「庁舎外連携」)と 4 のカテゴリー(『政治的意思決定』『事業』『組織化』『予算化』) が抽出された. カテゴリーの関係性は,構造化モデルとして示された. 結論:本研究では,自治体の健康づくり事業化のパートナーシップ形成プロセスが構造化された. 構造化モデルは,パートナー シップ形成に対する行政担当者自身の意識と行動の結果であることが示唆された.