Miracle-dz/UniFAR-FTUs huggingface dataset:
Seven Non-contiguous Intracellular Residues of the Lutropin/Choriogonadotropin Receptor Dictate the Rate of Agonist-induced Internalization and Its Sensitivity to Non-visual Arrestins*

The amino acid sequences of the human (h) and rat (r) lutropin/choriogonadotropin receptors (LHR) are 87% identical, but the rate of agonist-induced internalization of the hLHR is ∼7 times faster than that of the rLHR. Chimeras of the hLHR and the rLHR showed that this rate is dictated by the serpentine domain and the cytoplasmic tail. Further mutational analysis identified seven residues, two adjacent residues in the second intracellular loop (Val/Gln in the rLHR and Ile/His in the hLHR), four non-contiguous residues in the third intracellular loop (Arg/Gln/Thr/Pro in the rLHR and Lys/Arg/Met/Thr in the hLHR), and one in the C-terminal tail (Leu in the rLHR and Phe in the hLHR), that are necessary and sufficient to impart the slow rate of internalization of the rLHR and the fast rate of internalization of the hLHR. The internalization of the rLHR and the hLHR display different sensitivities to the non-visual arrestins. Therefore, we also tested if the simultaneous exchange of these seven residues resulted in the exchange of this property. Since this was found to be the case, we propose that these seven residues identified here form a non-visual arrestin-binding site.
Stochastic Stability of Neural Networks with Both Markovian Jump Parameters and Continuously Distributed Delays

The problem of stochastic stability is investigated for a class of neural networks with both Markovian jump parameters and continuously distributed delays. The jumping parameters are modeled as a continuous-time, finite-state Markov chain. By constructing appropriate Lyapunov-Krasovskii functionals, some novel stability conditions are obtained in terms of linear matrix inequalities (LMIs). The proposed LMI-based criteria are computationally efficient as they can be easily checked by using recently developed algorithms in solving LMIs. A numerical example is provided to show the effectiveness of the theoretical results and demonstrate the LMI criteria existed in the earlier literature fail. The results obtained in this paper improve and generalize those given in the previous literature.
Progress toward a 1 V pulse-driven ac Josephson voltage standard

We present a new record output voltage of 275 mV rms, which is a 25 % improvement over the maximum achieved with previous ac Josephson voltage standard circuits. We demonstrate operating margins for these circuits and use them to measure the harmonic distortion of a commercial digitizer. Having exceeded the threshold of 125 mV rms for a single array of Josephson junctions, we propose an eight-array circuit capable of achieving 1 V rms.
Comparative antimycobacterial activities of rifampin, rifapentine, and KRM-1648 against a collection of rifampin-resistant Mycobacterium tuberculosis isolates with known rpoB mutations

A collection of 24 rifampin-resistant clinical isolates of Mycobacterium tuberculosis with characterized RNA polymerase beta-subunit (rpoB) gene mutations was tested against the antimycobacterial agents rifampin, rifapentine, and KRM-1648 to correlate levels of resistance with specific rpoB genotypes. The results indicate that KRM-1648 is more active in vitro than rifampin and rifapentine, and its ability to overcome rifampin resistance in strains with four different genetic alterations may prove to be useful in understanding structure-function relationships.
Different surface glycoprotein patterns on human T‐, B‐ and leukemic‐lymphocytes

We have labeled the exposed surface glycoprotein of human blood T‐ and B‐lymphocytes and cells from patients with chronic lymphocytic leukemia by the galactose oxidase‐tritiated sodium borohydride method. The labeled glycoproteins were separated by polyacrylamide slab gel electrophoresis and visualized by autoradiography. The T‐ and B‐lymphocytes show different and characteristic surface glycoprotein patterns. The surface glcoprotein patterns of the leukemic cells differ from those of normal, non‐malignant lymphocytes. A relationship between the altered surface glycoprotein pattern of leukemic cells and the expression of leumemiaassociated antigens is discussed.
User Preferences for Auditory Device-driven Menu Navigation

A device-driven auditory menu interface that “pushes” the menu options to the user and requires minimal user input can be beneficial to users with both limited vision and input range. Presentation rate is important for auditory menus; they need to be slow enough for comprehension and responding, but fast enough to prevent frustration. User preferences for inter-item gap on an auditory device-driven cell phone menu were investigated. Participants (n=11) navigated a two-level auditory menu to target a randomly chosen item. Before each trial participants set the inter-item gap by manipulating a slider. Results showed a mean preference for . 853 second inter-item gap in experimental trials, and a significant decrease in preferences between practice (15) and experimental (30) trials [t(10) = 4.28, p = . 002]. Accuracy was 91.5% over all the trials. The findings indicate that it is feasible to design a limited-input cell phone with an auditory device-driven menu.
Immunocytochemical Studies of Atherosclerotic Lesions of Cerebral Berry Aneurysms

Abstract. Immunocytochemical analysis of human saccular aneurysms, commonly referred to as berry aneurysms, was performed on formalin-fixed, paraffin-embedded sections using monoclonal antibodies with single and double staining methods. Atherosclerotic lesions were detected in all aneurysms, which ranged in size from 2 mm to 3 cm in diameter. Changes consistent with the earliest stages of atherogenesis, so-called “fatty streaks,” were not detected. In the smallest aneurysms, atherosclerotic lesions were characterized by diffuse intimal thickening composed predominantly of proliferating smooth muscle cells (SMC) with a small number of macrophages and lymphocytes. Large aneurysms had advanced atherosclerotic lesions with cellular infiltrates composed mostly of macrophages, more mature looking SMC and a greater number of lymphocytes. Major histocompatibility complex (MHC) class II expression was detected predominantly in macrophages in all aneurysms. Some SMC in advanced atherosclerotic lesions, but not in diffuse intimal thickening, had MHC class II immunoreactivity. A significant number of lymphocytes and NK cells were found at the site of aneurysmal rupture. The progression of atherosclerosis within the aneurysmal sac correlated positively with aneurysmal growth, and we speculate may have contributed to aneurysmal rupture. Some evidence also suggested a possible role of atherosclerosis in the formation of berry aneurysms.
Bullous pemphigoid induced by ipilimumab in a patient with metastatic malignant melanoma after unsuccessful treatment with nivolumab

Dear Editor, Immune checkpoint inhibitors are used widely to treat several malignancies; however, immune-related adverse effects (irAE) often lead to cessation of use. Little is known about the autoimmune bullous disease induced by immune checkpoint inhibitors. Here, we report the case of ipilimumab-induced bullous pemphigoid (BP) after unsuccessful treatment of metastatic melanoma with nivolumab. A 35-year-old Japanese man, who had undergone resection of malignant melanoma on the right forearm 2 years and 6 months prior, presented with multiple metastases on the right lung. Seven cycles of i.v. nivolumab were administrated, however, metastasis to the liver and left cubital lymph node was detected 6 months later. Therefore, the patient received four courses of i.v. ipilimumab sequentially. After the second cycle of ipilimumab, the patient developed severe itching coupled with well-defined round erythema. Moreover, upon administration of the fourth cycle of ipilimumab, the skin lesions worsened and erythematous papules and plaques with tense bullae appeared on the extremities (Fig. 1a,b). Moderate
Birth weight and non-insulin dependent diabetes: thrifty genotype, thrifty phenotype, or surviving small baby genotype?

Abstract Objective : To determine the prevalence of diabetes in relation to birth weight in Pima Indians. Design : Follow up study of infants born during 1940-72 who had undergone a glucose tolerance test at ages 20-39 years. Setting : Gila River Indian community, Arizona. Subjects : 1179 American Indians. Main outcome measure: Prevalence of non-insulin dependent diabetes mellitus (plasma glucose concentration >=11.1 mmol/l two hours after ingestion of carbohydrate). Results : The prevalence was greatest in those with the lowest and highest birth weights. The age adjusted prevalences for birth weights <2500 g, 2500-4499 g, and >=4500 g were 30%, 17%, and 32%, respectively. When age, sex, body mass index, maternal diabetes during pregnancy, and birth year were controlled for, subjects with birth weights <2500 g had a higher rate than those with weights 2500-4499 g (odds ratio 3.81; 95% confidence interval 1.70 to 8.52). The risk for subsequent diabetes among higher birthweight infants (>=4500 g) was associated with maternal diabetes during pregnancy. Most diabetes, however, occurred in subjects with intermediate birth weights (2500-4500 g). Conclusions : The relation of the prevalence of diabetes to birth weight in the Pima Indians is U shaped and is related to parental diabetes. Low birth weight is associated with non-insulin dependent diabetes. Given the high mortality of low birthweight infants selective survival in infancy of those genetically predisposed to insulin resistance and diabetes provides an explanation for the observed relation between low birth weight and diabetes and the high prevalence of diabetes in many populations.
Evolution of transgenerational immunity in invertebrates

Over a decade ago, the discovery of transgenerational immunity in invertebrates shifted existing paradigms on the lack of sophistication of their immune system. Nonetheless, the prevalence of this trait and the ecological factors driving its evolution in invertebrates remain poorly understood. Here, we develop a theoretical host–parasite model and predict that long lifespan and low dispersal should promote the evolution of transgenerational immunity. We also predict that in species that produce both philopatric and dispersing individuals, it may pay to have a plastic allocation strategy with a higher transgenerational immunity investment in philopatric offspring because they are more likely to encounter locally adapted pathogens. We review all experimental studies published to date, comprising 21 invertebrate species in nine different orders, and we show that, as expected, longevity and dispersal correlate with the transfer of immunity to offspring. The validity of our prediction regarding the plasticity of investment in transgenerational immunity remains to be tested in invertebrates, but also in vertebrate species. We discuss the implications of our work for the study of the evolution of immunity, and we suggest further avenues of research to expand our knowledge of the impact of transgenerational immune protection in host–parasite interactions.
Structure of the Neisseria meningitidis Outer Membrane PilQ Secretin Complex at 12 Å Resolution*

The bacterial pathogen Neisseria meningitidis expresses long, thin, retractile fibers (called type IV pili) from its cell surface and uses these adhesive structures to mediate primary attachment to epithelial cells during host colonization and invasion. PilQ is an outer membrane protein complex that is essential for the translocation of these pili across the outer membrane. Here, we present the structure of the PilQ complex determined by cryoelectron microscopy to 12 Å resolution. The dominant feature of the structure is a large central cavity, formed by four arm features that spiral upwards from a squared ring base and meet to form a prominent cap region. The cavity, running through the center of the complex, is continuous and is effectively sealed at both the top and bottom. Analysis of the complex using self-orientation and by examination of two-dimensional crystals indicates a strong C4 rotational symmetry, with a much weaker C12 rotational symmetry, consistent with PilQ possessing true C4 symmetry with C12 quasi-symmetry. We therefore suggest that the complex is a homododecamer, formed by association of 12 PilQ polypeptide chains into a tetramer of trimers. The structure of the PilQ complex, with its large and well defined central chamber, suggests that it may not function solely as a passive portal in the outer membrane, but could be actively involved in mediating pilus assembly or modification.
Save the Global: Global Signal Connectivity as a Tool for Studying Clinical Populations with Functional Magnetic Resonance Imaging

The global signal is commonly removed from resting-state data, as it was presumed to reflect physiological noise. However, removal of the global signal is now under debate, as this signal may reflect important neuronal components, and its removal may introduce artifacts into the data. Here, we show that the functional connectivity (FC) of the global signal is of functional relevance, as it differentiates between schizophrenia patients and healthy controls during rest. We also demonstrate that other reported findings related to various clinical populations may actually reflect alternations in global signal FC. The evidence of the clinical relevance of the global signal propose its usage as a research tool, and extend previously reported perils of global signal removal in resting-state data of clinical populations.
Classification of behavior problems of children.

DSM-II subdivides behavior disorders of childhood and adolescence into seven categories: "hyperkinetic reaction," "withdrawing reaction," "overanxious reaction," "runaway reaction," "unsocialized aggressive reaction," and "group delinquent reaction," plus "other reaction. " The first six reactions represent clinically recognizable symptomatic clusters supported by research studies. The author outlines the problems of treating children in each of these groups, noting that the treatment can with advantage be individualized from a general pattern appropriate to the general type of problem.
Title Manganese-enhanced MRI of hypoxic-ischemic brain injuriesusing

In this study, Mn-dipyridoxaldiphosphate (MnDPDP), a clinically approved manganese contrast agent for hepatic and pancreatic imaging, was demonstrated for the first time for manganese-enhanced MRI (MEMRI) in brains of normal young rats (n=4) and rats with hypoxic-ischemic (H-I) insult at postnatal day 7 (n=8). After a single intraperitoneal injection of low dosage with 0. 1μmol/g in postnatal 14 days, 2D T1-weighted image (T1WIs), T1 maps, T2-weighted images (T2WIs) and T2 maps were acquired at 7 Tesla 1 day before, 1 day and 7 days after MnDPDP injection. The image contrast changes induced by MnDPDP appeared as the hyperintensity in T1WIs and the hypointensity in T2WIs. T1and T2 values decreased in the regions of Mn enhancement. Such enhancement presented as a delayed pattern that was more pronounced in 7 day after MnDPDP injection, suggesting the sustained Mn accumulation due to MnDPDP. Moreover, the MnDPDP enhancement in H-I brains was more pronounced in the lesion sites and was easily detectable in T1WI, T1 map, T2WI and T2 map. The results demonstrated here support the possibility of using MnDPDP as a ‘slow release’ Mn for clinical diagnosis of various neuropathologies.
La peau entre signature et biffure : du tatouage et du piercing aux scarifications

La peau est l’objet d’une revendication esthetique, mais simultanement, a travers la prise d’importance des attaques au corps chez les jeunes generations, a travers notamment les scarifications, elle incarne le lieu sensible de l’identite contemporaine, elle en traduit l’ambivalence, la frontiere ambigue et difficile entre soi et l’autre qui implique une mobilisation et un souci sans repos. La peau est saturee d’inconscient et de culture, elle devoile certes le psychisme du sujet, mais aussi la part qu’il prend a l’interieur du lien social, l’histoire qui le baigne.
Grandmothers migrating, working and caring: Latvian women between survival and self-realisation

Abstract This paper describes the circumstances surrounding the migration of older Latvian women and their multi-dimensional lives as economic migrants and as distant carers and supporters of diverse family members who remain in Latvia. In post-Soviet Latvia, especially since the 2008 financial crisis and the austerity measures which took away hope for a decent old-age pension, older women migrate abroad in order to salvage their economic wellbeing and support their multi-generation families, which can run to four generations – their children and grandchildren plus, often, their elderly parents. Migration enables these women to maintain multidirectional flows of care and also to achieve economic and psychosocial independence. Therefore, care practices that reach four generations put the figure of the grandmother at the core of transnational care relations. Research evidence for this paper comprises 50 in-depth interviews with older Latvian migrant women aged from their mid-40s to their late 60s in the UK and elsewhere. The paper demonstrates the complexity and richness of these women’s working lives, built around enhanced economic wellbeing, multiple and transnational caring responsibilities, and a new sense of self-worth and empowerment.
O efeito do reset no sistema estomatognático de portadores de disfunção temporomandibular

Introducao: O RESET e um metodo simples e nao invasivo de relaxamento dos musculos da mandibula constituido de 15 etapas de energizacao de determinadas partes do corpo. Sua aplicacao e indicada para individuos portadores de disfuncao temporomandibular. Objetivo: Verificar os efeitos do RESET nas estruturas e funcoes do sistema estomatognatico; nos sinais e sintomas de disfuncao temporomandibular, alem de investigar a manutencao dos resultados sem a aplicacao continuada da tecnica. Material e metodo: Estudo de quatro casos de adultos com diagnostico de disfuncao temporomandibular. Os participantes foram submetidos a avaliacao fonoaudiologica e 10 sessoes de RESET. Apos cinco sessoes, os participantes responderam um questionario de acompanhamento. Ao completar 10 sessoes, realizou-se reavaliacao. Decorridos 60 dias, submeteram-se a nova reavaliacao, para verificacao da manutencao dos resultados. Os resultados foram analisados de forma descritiva. Resultados: observou-se aumento dos movimentos de abertura, protrusao e lateralizacao mandibular; reducao dos ruidos articulares; adequacao da tensao de masseter, com diminuicao da dor neste musculo; reducao do habito de apertamento dentario, alem de sensacao de relaxamento geral. Alguns resultados foram mantidos por ate dois meses sem a aplicacao da tecnica, especialmente o aumento da abertura maxima de boca, a diminuicao da dor com consequente melhora das dificuldades causadas por esta e a sensacao de relaxamento geral. Conclusoes: Existem indicios de que o RESET seja uma terapia complementar eficaz no tratamento de disfuncao temporomandibular podendo ser utilizado como tecnica de relaxamento associada a terapia fonoaudiologica.
The Role of Fetal Growth Restriction and Gestational Age on Length of Hospital Stay in Twin Infants

Objective: To evaluate the association between length of gestation and fetal growth restriction (FGR) and the role of FGR in neonatal morbidity as determined by length of hospital stay among newborn twins. Methods: Using a race-, gender-, and gestational age-specific birth weight and length of hospital stay singleton standard, 490 twin infants were classified as FGR (at or below the tenth percentile) or non-FGR (above the tenth percentile). Length of stay and length-of-stay ratio (twin stay divided by the 50th percentile singleton stay) were calculated for each twin. Analysis included stepwise multiple regression for length of stay and length-of-stay ratio, and analysis of variance with main effects and second-order interactions for the length-of-stay measures using factors significant in the multiple regression models. Results: For length of stay, significant factors in the multiple regression model included respiratory distress syndrome (RDS), gestation category, FGR, sepsis, time of birth 1979-1984 versus 1985-1989), and cesarean delivery. For length-of-stay ratio, significant factors included FGR, gestation category, sepsis, period of birth, cesarean delivery, and hyperbilirubinemia. The analysis of variance showed that RDS had the greatest effect on length of stay, whereas FGR had the greatest effect on length-of-stay ratio. For both dependent variables, the effect of FGR was magnified when compounded by significant neonatal complications (RDS, sepsis, hyperbilirubinemia) or gestation category. Conclusions: Fetal growth restriction is a major factor in the neonatal morbidity of twins. Early recognition of and interventions for FGR in twin gestations should be a primary goal for reducing both immediate and long-term adverse outcomes.
Vector corrected noise temperature measurements

A new one-port technique for measuring noise temperature is presented that uses receiver noise parameters for error correction. Improved accuracy in one-port measurements of noise temperature made with commercial systems is demonstrated without using isolators. Equations for correcting mismatch errors are developed as part of the available vector noise temperature equation. Results, presented for a C-band solid-state cold noise source and a pair of microwave solid-state noise diodes, are shown to be in good agreement with radiometric measurements of the same sources.
Quantitative evaluation of leukocyte dynamics in retinal microcirculation.

PURPOSE Leukocyte rheology may play a key role in microcirculation because leukocytes have unique properties, such as large cell volume, high cytoplasmic rigidity, and low deformability. However, only a few methods are available to study the dynamic behavior of leukocytes in retinal microcirculation. The authors developed a new method to analyze directly movements of leukocytes in the retinal vessels of primates. METHODS Acridine orange, which has been used as a nuclear stain in histochemistry and cytochemistry, was injected intravenously into cynomolgus monkeys for a vital staining of leukocytes. The fundus image was generated with the argon blue laser and a scanning laser ophthalmoscope. The images were recorded on a magnetic tape and evaluated with a personal computer-based image analysis system. RESULTS Each leukocyte was recognized as a single fluorescent dot moving in the retinal vessels. It was possible to analyze the spatial and temporal dynamics of individual leukocytes in the capillaries. Some leukocytes passed through the capillaries, plugging transiently under the physiological condition. Leukocytes that stayed in the same position for a few minutes may have stuck to the endothelium as a result of leukocyte-endothelial interactions. In the postcapillary vessels, leukocytes tended to be displaced from the center stream toward the vessel walls. The mean flow velocity of leukocytes in the perifoveal capillary was 0.92 +/- 0.32 mm/sec. CONCLUSIONS This study clearly demonstrated that rheologic behaviors of leukocytes in the retinal microcirculation can be studied through the vital staining with acridine orange in vivo. The authors' results suggest a potential role of leukocytes in retinal vascular flow disturbances. This study may open the door to the investigation of leukocyte hemodynamics in the retinal microcirculation in vivo.