Miracle-dz/UniFAR-FTUs huggingface dataset:
Blood Vessels Disruption of K 2 P 6 . 1 Produces Vascular Dysfunction and Hypertension in Mice

K2P6.1, a member of the 2-pore domain K channel family, is highly expressed in the vascular system; however, its function is unknown. We tested the following hypotheses. K2P6.1 regulates the following: (1) systemic blood pressure; (2) the contractile state of arteries; (3) vascular smooth muscle cell migration; (4) proliferation; and/or (5) volume regulation. Mice lacking K2P6.1 (KO) were generated by deleting exon 1 of Kcnk6. Mean arterial blood pressure in both anesthetized and awake KO mice was increased by 17 2 and 26 3 mm Hg, respectively (P 0.05). The resting membrane potential in freshly dispersed vascular smooth muscle cells was depolarized by 17 2 mV in the KO compared with wild-type littermates (P 0.05). The contractile responses to KCl (P 0.05) and BAY K 8644 (P 0.01), an activator of L-type calcium channels, were enhanced in isolated segments of aorta from KO mice. However, there was no difference in the current density of L-type calcium channels. Responses to U46619, an agent that activates rho kinase, showed an enhanced contraction in aorta from KO mice (P 0.001). The BAY K 8644-mediated increase in contraction was decreased to wild-type levels when treated with Y27632, a rho kinase inhibitor, (P 0.05). K2P6.1 does not appear to be involved with migration, proliferation, or volume regulation in cultured vascular smooth muscle cells. We conclude that K2P6.1 deficiency induces vascular dysfunction and hypertension through a mechanism that may involve smooth muscle cell depolarization and enhanced rho kinase activity. (Hypertension. 2011;58:672-678.) ● Online Data Supplement
A novel timing jitter robust UWB impulse radio system

Traditional ultra-wideband (UWB) impulse radio system is sensitive to timing jitter effect, as a result precise synchronization is required. Our previous study shows that a simple "sample and compare'' high-speed UWB receiver is an effective scheme to combat timing jitter. In this paper, we extend our previous study by using the maximum absolute sampling value as the decision variable and derive the corresponding threshold optimization algorithm by means of a version of the Kiefer-Wolfowitz algorithm with expanding truncations and randomized differences. The new algorithm is robust under timing jitter and no knowledge of the channel gains is required. Convergence of the algorithm is analyzed. Simulation results show that the new algorithm has better performance than previous one
Container-based network function virtualization for software-defined networks

Today's enterprise networks almost ubiquitously deploy middlebox services to improve in-network security and performance. Although virtualization of middleboxes attracts a significant attention, studies show that such implementations are still proprietary and deployed in a static manner at the boundaries of organisations, hindering open innovation. In this paper, we present an open framework to create, deploy and manage virtual network functions (NF)s in OpenFlow-enabled networks. We exploit container-based NFs to achieve low performance overhead, fast deployment and high reusability missing from today's NFV deployments. Through an SDN northbound API, NFs can be instantiated, traffic can be steered through the desired policy chain and applications can raise notifications. We demonstrate the systems operation through the development of exemplar NFs from common Operating System utility binaries, and we show that container-based NFV improves function instantiation time by up to 68% over existing hypervisor-based alternatives, and scales to one hundred co-located NFs while incurring sub-millisecond latency.
Simulating Biological Neural Networks to investigate the Membrane Potential Bistability phenomenon Final Report

Science is nowhere near to fully understanding the functional model of the brain. Although medicine has advanced enormously over the past decade, most parts of the brain still remain a mystery as to how they function, interact and solve complex problems. Another unanswered question is what happens in the brain during sleep. Medicine has clearly indicated that sleep is vital for the proper functioning of our daily lives. Yet little is known which brain processes are set in motion during sleep, and what goal they aim to fulfill. Recent experiments have shown that during sleep neurons behave in a peculiar way; they show a bimodal state system, in which they are either in an “up-state” or a “down-state”. Neuroscientist commonly refer to this as the bistability phenomenon. Our research project will aim to explore the computational tools to simulate this behavior using computational simulations. We will describe how we created an easy to use simulation environment that is made for the purpose of further researching this phenomenon with the goal to further understand its behavior under various different situations. We hope our research will bring science closer to understanding the complexities of the brain.
Analyse temps-fréquence en mécanique cellulaire et adaptabilité du fuseau mitotique

Le fuseau mitotique assure la segregation des chromatides sœurs et le maintien de la poidie des cellules filles. Le fuseau est compose de microtubules dynamiques (qui polymerisent et depolymerisent continuellement), de nombreux moteurs moleculaires, d'agents de reticulations et de regulateurs. Bien que la structure du fuseau au niveau moleculaire soit connue, son fonctionnement reste delicat a comprendre, et necessite la prise en compte de la dynamique de ses composants et leurs interactions. Les approches utilisees pour repondre a ces problematiques sont jusqu'a maintenant plutot des approches in silico et in vitro. Il manque aujourd'hui une caracterisation de la mecanique du fuseau dans son contexte physiologique. Nous proposons une methode non invasive basee sur de l'analyse d'image, combine a une modelisation heuristique pour mesurer les parametres mecaniques durant toute la division. Nous suivons les poles du fuseau marques par proteine fluorescente avec un taux acquisition rapide et une bonne resolution spatiale ce qui nous permet d'acceder aux fluctuations de longueur du fuseau in vivo. Avec la transformee de Fourier aux temps courts, nous calculons leurs densites spectrales de puissances — leurs signatures mecaniques. Ces spectres sont alors ajustes avec un modele Kelvin — Voigt avec inertie (un ressort, un amortisseur et un terme inertiel en parallele). Nous avons valide la methode par des experiences numeriques ou nous retrouvons les evolutions des parametres sur des donnees simulees et la calibration a ete realisee par l'utilisation de la rupture du fuseau induite par micro chirurgie laser ou par la genetique. Nous avons caracterise le fuseau de l'embryon unicellulaire du nematode C. elegans. La methaphase apparait dominee par l'amortisseur, ce qui est coherent avec la lente elongation du fuseau que nous observons. Mais contraste l'idee repandue de l'existence d'un mecanisme de maintien de la longueur du fuseau durant la metaphase. Au passage en anaphase, les trois parametres mecaniques chutent, avant de reaugmenter environ 50 secondes apres la transition pour reatindre un regime domine de nouveau par l'amortisseur, ce qui suggere que les microtubules interpolaires jouent un role mineur durant l'elongation du fuseau en debut d'anaphase. Dans la perspective de comprendre le lien entre la mecanique du fuseau et les interactions des acteurs moleculaires, nous avons partiellement supprime un gene par sous-structure du fuseau. Nous avons alors retrouve des comportements connus avec une perspective augmentee offerte par notre methode. Cette methode, ne va pas seulement permettre la comprehension fondamentale de la mecanique du fuseau, en remplacant la modelisation du fuseau base uniquement sur la longueur, mais aussi d'aller vers la prise en compte de la robustesse de fonctionnement du fuseau mitotique face aux defauts tel que la polyou l'aneuploidie.
BrUGE1 transgenic rice showed improved growth performance with enhanced drought tolerance

UDP-glucose 4-epimerase (UGE) catalyzes the reversible conversion of UDP-glucose to UDP-galactose. To understand the biological function of UGE from Brassica rapa, the gene BrUGE1 was cloned and introduced into the genome of wild type rice ‘Gopum’ using the Agrobacterium-mediated transformation method. Four lines which carried a single copy gene were selected and forwarded to T3 generation. Agronomic traits evaluation of the transgenic T3 lines (CB01, CB03, and CB06) under optimal field conditions revealed enriched biomass production particularly in panicle length, number of productive tillers, number of spikelets per panicle, and filled spikelets. These remarkably improved agronomic traits were ascribed to a higher photosynthetic rate complemented with higher CO2 assimilation. Transcripts of BrUGE1 in transgenic lines continuously accumulated at higher levels after the 20% PEG6000 treatment, implying its probable role in drought stress regulation. This was paralleled by rapid accumulation of soluble sugars which act as osmoprotectants, leading to delayed leaf rolling and drying. Our findings suggest the potential of BrUGE1 in improving rice growth performance under optimal and water deficit conditions.
Calcium Treatment for Premenstrual Syndrome

OBJECTIVE: TO evaluate the use of calcium supplementation in the treatment of premenstrual syndrome. DATA SOURCES: Clinical literature accessed through MEDLINE (from January 1967 to September 1999). Key search terms included calcium, PMS, and premenstrual. DATA SYNTHESIS: Up to 50% of women experience some form of premenstrual syndrome. An evaluation of studies focusing on calcium in the management of premenstrual symptoms was conducted. CONCLUSIONS: Calcium supplementation of 1200–1600 mg/d, unless contraindicated, should be considered a sound treatment option in women who experience premenstrual syndrome. The supplemental dose of calcium can be adjusted downward in the few patients who routinely consume large quantities of calcium in their diet.
Growth/differentiation factor-5 modulates the synthesis and expression of extracellular matrix and cell-adhesion-related molecules of rat Achilles tendon fibroblasts

This study was designed to examine the cellular and molecular response of tendon fibroblasts to growth/differentiation factor-5 (GDF-5). Rat Achilles tendon fibroblasts (ATFs) were treated in culture with varying concentrations of GDF-5 (0–1000 ng/ml) over varying periods of time (0–12 days). Cell proliferation, evaluated through use of a standard MTT colorimetric assay, confirmed that GDF-5 stimulates ATF proliferation in a concentration- and time-dependent fashion. Temporal and concentration analysis revealed that GDF-5 increases total DNA, glycosaminoglycan (GAG), and hydroxyproline (HYP) content. Ratios of HYP/DNA and GAG/DNA increased with increasing concentrations of GDF-5 (0–1000 ng/ml). Expression of the following 12 extracellular matrix (ECM) and cell-adhesion-related genes was assessed using real-time reverse transcriptase polymerase chain reaction (RT-PCR): collagen I (col I), collagen III (col III), matrix metalloproteinases (MMP)-3 and -13, aggrecan, tissue inhibitor of matrix metalloproteinase (TIMP)-2, syndecan-4, N-cadherin, tenascin-C, biglycan, versican, and decorin. RT-PCR data revealed an increase in the expression of col I, col III, MMP-3, MMP-13, TIMP-2, syndecan-4, N-cadherin, tenascin-C, and aggrecan genes by day 6. A statistically significant decrease in TIMP-2 and MMP-13 was observed on day 12. Decorin expression was depressed at all time points in cells treated with GDF-5. There was no significant change in biglycan expression in ATFs supplemented with GDF-5. These findings suggest that GDF-5 induces cellular proliferation and ECM synthesis as well as expression of ECM and cell-adhesion-related genes in ATFs. This study further defines the influence of GDF-5 on rat ATFs through its action on the expression of genes that are associated with tendon ECM.
Internal Iliac Artery Ligation versus Local Injection of Vasopressin in Placental site in Placenta Previa during Cesarean section to Reduce Blood Loss

Introduction Placenta previa is characterized by the abnormal placenta overlying the endocervical os, and it is known as one of the most feared adverse maternal and fetal-neonatal complications in obstetrics (1) . The incidence of placenta previa has been recently increased over the past few decades, correlating with the elevated caesarean section rate (2) . For management of pregnant women with placenta previa, cesarean section is recommended as the mode of delivery (3) . Placenta previa is a well-known cause of massive intrapartum hemorrhage that is associated with high mortality and morbidity for both the mother and the neonate (4) . For bleeding from the placental separation surface, various hemostatic approaches have been proposed and put into practice (5) . Many of the techniques that have been developed to minimize intraoperative blood loss have focused on reducing pelvic circulation by bilateral ligation of internal iliac arteries or their branches (6) . Many gynecological surgeons use a local injection of vasopressin, which is a known peripheral vasoconstrictor, at the time of laparoscopic myomectomy to decrease blood loss. In addition, the useful role for local infiltration of vasopressin to arrest hemorrhage from the placental bed has been demonstrated in several obstetrical case reports (7) .
Desarrollo y evaluación de arquitecturas lógicas basadas en Nanopipeline.

El potencial de la logica dinamica, con sus fases de precarga y evaluacion es una solucion muy estudiada y aplicada, para implementar pipeline sin elementos de memoria usando un esquema de reloj de multiples fases solapadas (superpipeline). Se sabe que se han desarrollado distintas variantes de este superpipeline y aplicado en entornos y productos comerciales Sin embargo, el escalado tecnologico y las altas frecuencias en las que se trabaja actualmente han aumentado los principales problemas asociados a este estilo logico. En primer lugar, en las tecnologias DSM, el diseno de puertas dinamicas robustas y rapidas se ha convertido en una tarea dificil debido al gran incremento de las corrientes de fuga y sub-umbrales, los acoplos de senal, las fluctuaciones de potencia y la variabilidad. En segundo lugar, se trata de dificultades relacionadas con la interconexion de puertas para formar redes logicas, como su caracter no inversor o la problematica del diseno con un nivel de puertas por fase (nano-pipelines) con dos fases de reloj. El trabajo realizado en el marco de esta Tesis Doctoral tiene como objetivo general estudiar en profundidad la problematica de los nanopipelines y explorar soluciones a nivel arquitectural y a nivel electrico para la realizacion de unidades funcionales aritmetico-logicas competitivas en aplicaciones de altas prestaciones en tecnologias DSM. Las principales aportaciones y resultados obtenidos son: Desarrollo y validacion experimental de una nueva topologia de puerta dinamica DOE. Presenta ventajas, en terminos de compromisos entre la velocidad y tolerancia al ruido, con respecto a la solucion convencional Domino. Para una misma tolerancia al ruido razonable, el retraso de una NOR con un fan_in alto Domino es un 77% en 32nm y un 95% en 16nm superior al de DOE. Evaluacion de superpipelines Domino con distinta profundidad logica. Los resultados obtenidos muestran que estas arquitecturas se benefician de aplicar un pipeline muy fino. La comparacion de sumadores Kogge Stone en una tecnologia DSM, usando tres puertas por fase de reloj y una puerta por fase (nanopipeline), muestra como esta ultima puede operar un 50% mas rapido con similar energia por operacion, incrementar la fortaleza del keeper un 60% sin degradar la velocidad, ni la energia, o reducir un 25% el consumo sin degradar velocidad ni tolerancia al ruido, entre otros compromisos de diseno. Analisis de la operacion de arquitecturas nanopipelines Domino y DOE. La topologia DOE presenta ventajas respecto a Domino debido a su caracter inversor. Los nanopipelines DOE son mas robustos frente a variaciones de parametros de dispositivos y de operacion y a no idealidades del reloj. Se simplifica considerable del esfuerzo de diseno de estas arquitecturas para operacion con dos fases de reloj. Validacion experimental de nanopipelines DOE.
Versuchsplanung und Methoden zur Identifikation zeitkontinuierlicher Zustandsraummodelle am Beispiel des Verbrennungsmotors

Diese Arbeit beschreibt neue Methoden der Online-Versuchsplanung und Online-Identifikation dynamischer Zusammenhange am Verbrennungsmotorenprufstand. Diese Methoden sollen die Steuergerateapplikation bei der Modellierung und Optimierung dynamischer Vorgange unterstutzen. Die Methoden der Versuchsplanung ermoglichen es unter Berucksichtigung von Einschrankungen an die Ein- und Ausgange die Versuchsplanung hinsichtlich des spateren Einsatzzwecks der Modelle zu optimieren. Die Modellbildung ermoglicht die Identifikation eingangs-/zustandsstabiler zeitkontinuierlicher Zustandsraummodelle unter Verwendung von Neuronalen Netzen, die mittels Runge-Kutta Methoden zeitdiskretisiert werden. Es kann gezeigt werden, dass sowohl die Methoden der Versuchsplanung als auch die der Modellbildung den bisher am Verbrennungsmotorenprufstand eingesetzten Methoden uberlegen sind.
Lesions of T‐Cell‐Mediated Kidney Allograft Rejection in Mice Do Not Require Perforin or Granzymes A and B

Organ allograft rejection is strongly associated with the presence of alloreactive cytotoxic T cells but the role of cytotoxicity in the pathologic lesions is unclear. Previous studies showed that the principal lesions of kidney rejection – interstitial infiltration, tubulitis, and endothelial arteritis – are T‐cell‐dependent and antibody‐independent. We studied the role of cytotoxic granule components perforin and granzymes A and B in the evolution of the T‐cell‐mediated lesions of mouse kidney transplant rejection. By real‐time RT‐PCR, allografts rejecting in wild‐type hosts at days 5, 7, 21, and 42 showed massively elevated and persistent expression of perforin and granzymes A and B, but evolution of tubulitis and arteritis did not correlate with increasing granzyme or perforin expression. Allografts transplanted into hosts with disrupted genes for perforin or granzymes A and B showed no change in tubulitis, arteritis, or MHC induction. Thus the development of the histologic lesions diagnostic of T‐cell‐mediated kidney transplant rejection are associated with but not mediated by perforin or granzyme A or B. Together with previous graft survival studies, these results indicate that the granule‐associated cytotoxic mechanisms of T cells are not the effectors of T‐cell‐mediated allograft rejection.
Successful treatment of left atrial disk thrombus on an Amplatzer atrial septal defect occluder with abciximab and heparin

Acute device thrombosis is a rare but important complication after transcatheter atrial septal defect closure. In this case a mobile thrombus was noted on the left side of an Amplatzer atrial septal occluder after device release in a 12 year old boy with an uncomplicated atrial septal defect. The thrombus was successfully treated with an infusion of heparin and the glycoprotein IIa/IIIb receptor antagonist abciximab. Transoesophageal ultrasound performed the following day showed complete resolution of the clot. There are no reports to guide treatment of acute thrombosis in this setting. This combination of treatments was effective without complication in this case.
UniLex: Método Léxico para Análise de Sentimentos Textuais sobre Conteúdo de Tweets em Português Brasileiro

Este artigo aborda a criacao de um metodo computacional baseado em dicionario lexico, que seja capaz de analisar textos em idioma portugues brasileiro e de polarizar as palavras de acordo com os sentimentos por elas transmitidos. O estudo realizado visa detectar os sentimentos expressos nos textos da Web e comparar a abordagem proposta com metodos existentes, utilizando a aplicacao iFeel, que retorna os resultados dos principais metodos para a analise de sentimentos textuais. Pode-se concluir que, por meio de experimentos em uma base de dados do Twitter em portugues brasileiro, o metodo proposto retorna os sentimentos associados nos textos analisados com uma acuracia melhor do que os metodos descritos em trabalhos relacionados.
Wireless paper-based biosensor reader for the detection of infectious diseases at the point of care

A handheld wireless optical reader for the detection of infectious diseases at the point of care is presented. The device is battery powered and interfaces with paper-based biosensors for the quantification of concentration in biological fluids using colorimetric analysis. The results are wirelessly transmitted to a smartphone. The device has been successfully tested with various concentrations of Influenza A nucleoprotein.
Suppressed catalytic activity of base excision repair enzymes on rotationally positioned uracil in nucleosomes

The majority of DNA in eukaryotic cells exists in the highly condensed structural hierarchy of chromatin, which presents a challenge to DNA repair enzymes in that recognition, incision, and restoration of the original sequence at most sites must take place within these structural constraints. To test base excision repair (BER) activities on chromatin substrates, an in vitro system was developed that uses human uracil DNA glycosylase (UDG), apyrimidinic/apurinic endonuclease (APE), and DNA polymerase β (pol β) on homogeneously damaged, rotationally positioned DNA in nucleosomes. We find that UDG and APE carry out their combined catalytic activities with reduced efficiency on nucleosome substrates (≈10% of that on naked DNA). Furthermore, these enzymes distinguish between two different rotational settings of the lesion on the histone surface, showing a 2- to 3-fold difference in activity between uracil facing ``toward'' and ``away from'' the histones. However, UDG and APE will digest such substrates to completion in a concentration-dependent manner. Conversely, the synthesis activity of pol β is inhibited completely by nucleosome substrates and is independent of enzyme concentration. These results suggest that the first two steps of BER, UDG and APE, may occur ``unassisted'' in chromatin, whereas downstream factors in this pathway (i.e., pol β) may require nucleosome remodeling for efficient DNA BER in at least some regions of chromatin in eukaryotic cells.
Les codes à métrique de rang et leurs applications dans les réseaux Smart Grid

Cette these a pour cadre les transmissions sur les reseaux CPL-BE et les reseaux de capteurs a faible capacite. L'etat de l'art classique sur la protection de l'information dans la transmission par reseaux de capteurs fait reference a l'utilisation de codage distribue ou les relais implementent des operations de parite (melange des flux) sur les data issues des capteurs. Cependant, il est difficile, de par la nature variable de la qualite des liens en liaisons sans fil, de controler la qualite du codeur equivalent construit et de maintenir ses performances au cours du temps. C'est pourquoi nous nous sommes orientes dans cette these vers la recherche de schemas de codage differents qui resistent mieux a la variation de qualite des liaisons a travers le reseau. Notre choix s'est porte sur le codage par sous-espace inspire des travaux de Gabidulin. Le but est de former un code qui utilise une metrique simple et resistante pour securiser les transmissions sur le reseau. Les codes a metrique de rang repondent bien a ce besoin car il n'y a qu'a controler le rang de la matrice obtenue en reception pour verifier l'integrite de la transmission. Les codes a metrique de rang et leur algorithme de decodage ont ete etudies dans un premier temps. Puis, les performances du code LRPC propose concatene avec les codes convolutifs sont testees dans des schemas de transmission des contextes differents.