Miracle-dz/UniFAR-FTUs huggingface dataset:
Revised regional model life tables at very low levels of mortality.

This paper presents and discusses new model life tables at very low mortality, which make use of age-specific death rates from the 1960s, 1970s, and 1980s. These life tables fit recorded death rates in very low mortality populations better than do the existing ones at expectations of life of 77.5 and 80 years. The old tables incorporate too-high mortality at the higher ages and in infancy and they incorporate regional differences that no longer exist. The new tables "close out" the mortality schedules above age 80 more realistically. The convergence of age patterns of mortality at very high life expectancies in populations that used to conform to different families is in itself of demographic interest. Some convergence may perhaps be expected. Sullivan (1973) found that, in Taiwan, the comparison of mortality at ages 1-5 to mortality at 5-35 in the late 1950s showed higher mortality at the younger ages relative to the ensuing 30-year age interval than was found in any of the models, including the South model, which has the highest relative mortality from ages 1-5 among the 4 regional patterns. Then, in the late 1960s, the relation of mortality at 1-5 to mortality at 5-35 in Taiwan fell to a position intermediate between the West and South tables. Sullivan found in data on mortality by cause of death a large reduction in mortality from diarrhea and enteritis, no doubt as a result of environmental sanitation. Mortality from these causes is concentrated among young children, and reduction in deaths from these causes would naturally diminish the excess mortality in this age interval. The East pattern, characterized by very high mortality in infancy (but not from 1-5), may be the result of the prevalence of early weaning or avoidance of breast feeding altogether in the populations characterized by this pattern. As health conditions have improved, evidenced by the overall design of mortality, these special factors are diminished or erased. Model life tables at these very low mortality levels have different uses from most applications of model life tables at higher mortality. The use of model tables to estimate accurate schedules of mortality when the basic data are incomplete or inaccurate is less relevant in this range of mortality levels.
Validation of an Electronic, Population-Based Prescription Database

BACKGROUND: The Drug Programs Information Network (DPIN), Manitoba's (Canada) new electronic prescription database, is a valuable data source for pharmacoepidemiologic research. Pharmacies are required to submit to the DPIN all prescriptions for Pharmacare, the province's drug insurance plan, but submission of prescriptions for social assistance recipients and treaty status Indians is discretionary. OBJECTIVE: The completeness of the DPIN prescription database was assessed to determine whether treaty status Indians and social assistance recipients were underrepresented. DESIGN: Prescriptions dispensed during March 13–17, 1995, in a stratified sample of Manitoba pharmacies were linked to DPIN by prescription number to determine the proportions submitted for Indian Affairs, Social Services, and Pharmacare recipients. Pharmacare records in the DPIN were compared with original pharmacy records to evaluate data accuracy. RESULTS: Of 2196 Indian Affairs and 1879 Social Services prescriptions dispensed in 58 pharmacies, a corresponding prescription was found in the DPIN for 79.7% (98% CI 78.0% to 81.4%) and 90.1% (98% CI 88.8% to 91.4%) of prescriptions, respectively. These proportions were significantly lower than the estimated proportion of Pharmacare prescriptions submitted (93%, 98% CI 92.4% to 93.6%). Ninety-two percent of 8012 DPIN Pharmacare prescriptions matched the original prescription on the drug name, quantity, and days' supply. CONCLUSIONS: This study established that the DPIN is a valid and reliable data source for studying prescription use among the majority of Manitoban residents. However, the DPIN database has differential validity and underrepresents prescriptions dispensed for the aboriginal population.
Methicillin-Susceptible, Vancomycin-Resistant Staphylococcus aureus, Brazil

We report characterization of a methicillin-susceptible, vancomycin-resistant bloodstream isolate of Staphylococcus aureus recovered from a patient in Brazil. Emergence of vancomycin resistance in methicillin-susceptible S. aureus would indicate that this resistance trait might be poised to disseminate more rapidly among S. aureus and represents a major public health threat.
An algorithmic approach to the improvement of efficiency for surface mounting machines

With rapid development of the surface mounting technology (SMT), continual efforts have been made to make the surface mounting machines efficient as well as reliable. The authors propose an algorithmic approach to the improvement of efficiency for surface mounting machines. For minimizing the set-up time, a CAD data conversion algorithm, which makes the data for the pick-and-place sequences from the CADSTAR output files, is proposed. Also, for optimizing the feeder arrangement and the traveling path of heads in the surface mounting machine, SCM 130 model, effective feeder arrangement algorithms are proposed. To evaluate their performance, the proposed algorithms are tested on the assembly tasks of PCBs manufactured through simulations. As a result, it is shown that the proposed algorithm reduces the time required to perform the assembly sequences by maximum 9 percent when the task time is compared with the time required to perform the assembly sequences made by an expert.
Introduction of a Tryptophan Reporter Group into the ATP Binding Motif of the Escherichia coli UvrB Protein for the Study of Nucleotide Binding and Conformational Dynamics*

The DNA-dependent ATPase activity of UvrB is required to support preincision steps in nucleotide excision repair in Escherichia coli. This activity is, however, cryptic. Elicited in nucleotide excision repair by association with the UvrA protein, it may also be unmasked by a specific proteolysis eliminating the C-terminal domain of UvrB (generating UvrB*). We introduced fluorescent reporter groups (tryptophan replacing Phe47 or Asn51) into the ATP binding motif of UvrB, without significant alteration of behavior, to study both nucleotide binding and those conformational changes expected to be essential to function. The inserted tryptophans occupy moderately hydrophobic, although potentially heterogeneous, environments as evidenced by fluorescence emission and time-resolved decay characteristics, yet are accessible to the diffusible quencher acrylamide. Activation, via specific proteolysis, is accompanied by conformational change at the ATP binding site, with multiple changes in emission spectra and a greater shielding of the tryptophans from diffusible quencher. Titration of tryptophan fluorescence with ATP has revealed that, although catalytically incompetent, UvrB can bind ATP and bind with an affinity equal to that of the active UvrB* form (K d of ∼1 mm). The ATP binding site of UvrB is therefore functional and accessible, suggesting that conformational change either brings amino acid residues into proper alignment for catalysis and/or enables response to effector DNA.
Effect of melatonin on the oxidative stress in erythrocytes of healthy young and elderly subjects *

Abstract: The disturbances in pro‐ and antioxidant balance may play an important role in the pathomechanism of aging. The pineal hormone melatonin, which exerts effective antioxidative properties, is suggested to be involved in the aging process. The aim of this study was to compare the oxidative stress in erythrocytes of healthy young adults and elderly people, and to determine the influence of melatonin supplementation on measured parameters in both examined groups. The malondialdehyde (MDA) and reduced glutathione levels as well as Cu‐Zn superoxide dismutase (SOD‐1), catalase, glutathione peroxidase (GSH‐Px), glutathione S‐transferase (GST) and glutathione reductase (GR) activities in erythrocytes and morning serum melatonin concentration in 14 healthy young adults and 14 healthy elderly people at baseline and after the 30th day of melatonin (5 mg daily) supplementation were determined. A significant age effect on increasing the MDA level and decreasing SOD‐1, GSH‐Px and GR activities as well as melatonin concentration was observed. Melatonin supplementation resulted in a significant increase in melatonin concentration, SOD‐1 and GR activities and a decrease in the MDA level in both examined groups. These data indicate an age‐related augmentation of oxidative stress in erythrocytes and the improvement of erythrocytic antioxidative defense by melatonin administration. These results might suggest melatonin supplementation to prevent age‐related diseases and to prolong the lifespan and improve the quality of life of elderly people.
Therapeutic Drug Monitoring of Daptomycin: A Retrospective Monocentric Analysis

Background: Daptomycin dose is adjusted to body weight and renal function and is usually not guided by therapeutic drug monitoring. Daptomycin plasma concentration measurement was established at our institution in January 2009 and is now increasingly being used. The aim of this study was to describe and characterize variability in daptomycin exposure during routine clinical therapy. Methods: We collected daptomycin plasma concentrations that were measured at our institution during the period January 2009–July 2012. Additional clinical and demographic data and their association with daptomycin exposure were tested by a multilevel linear regression analysis. Results: A total of 332 daptomycin plasma concentrations were determined in 86 patients. Sixty-six percent (n = 218) of all determinations were trough concentrations (Cmin), and 34% (n = 114) were peak concentrations (Cmax). Cmin ranged 2–68 mg/L (median, 16.7 mg/L), and Cmax 20–236 mg/L (median, 66.2 mg/L). A significant positive association of total dose, albumin, creatinine and a significant negative association of dose interval and intermittent hemodialysis with Cmin were found in the regression analysis. Total dose and intensive care unit (ICU) stay were significantly associated with Cmax (P < 0.05). However, only 28% (P < 0.005) of Cmin variability and 8% (P = 0.08) of Cmax variability were explained by the factors included in the analysis. Conclusions: Daptomycin plasma concentrations are often unpredictable as shown by highly variable drug exposure that is only partially explained by dose administered and underlying renal function.
Modified backoff technique in fiber-wireless networks

A rapid development of broadband telecommunication services has strongly motivated the necessity of upgrading the access network infrastructure. As of now, network traffic load remains a bottleneck in efforts to deliver video, voice as well as advanced internet applications to residential customers due to the limitation of available bandwidth. By integrating gigabits passive optical network (GPON) with wireless local area network (WLAN) it is expected to meet the bandwidth demand by end users. However, interference from the wireless access still limits the end users' performance. Hence, this work aims to enhance the WLAN performance in the proposed fiber-wireless networks by utilizing the interoperability of these two networks. In this paper, we modify the legacy binary exponential backoff (BEB) algorithm in WLAN by introducing a constant contention window (CW) size. The optimized CW size is determined by analyzing the total traffic load passing through the GPON network. In comparison to the existing techniques, our scheme shows up to 50% improvement in throughput performance and nearly 5× reduction of media access delay when the scheme is heavily loaded.
Enhancement of natural killer (NK) cell cytotoxicity by fever‐range thermal stress is dependent on NKG2D function and is associated with plasma membrane NKG2D clustering and increased expression of MICA on target cells

Circulating NK cells normally experience temperature gradients as they move about the body, but the onset of inflammation can expose them and their targets to febrile temperatures for several hours. We found that exposure of human peripheral blood NK cells and target cells to fever‐range temperatures significantly enhances lysis of Colo205 target cells. A similar effect was not observed when NK cell lines or IL‐2‐activated peripheral blood NK cells were used as effectors, indicating that thermal sensitivity of effectors is maturation or activation state‐dependent. Use of blocking antibodies revealed that this effect is also dependent on the function of the activating receptor NKG2D and its ligand MHC class I‐related chain A (MICA). On NK cells, it was observed that thermal exposure does not affect the total level of NKG2D surface expression, but does result in its distinct clustering, identical to that which occurs following IL‐2‐induced activation. On tumor target cells, a similar, mild temperature elevation results in transcriptional up‐regulation of MICA in a manner that correlates with increased sensitivity to cytolysis. Overall, these data reveal that NK cells possess thermally responsive regulatory elements, which facilitate their ability to capitalize on reciprocal, stress‐induced changes simultaneously occurring on target cells during inflammation and fever.
Semantic Priming in Parkinsons Disease: Evidence for Delayed Spreading Activation

Nineteen persons with Parkinsons disease (PD) and 19 matched control participants completed a battery of online lexical decision tasks designed to isolate the automatic and attentional aspects of semantic activation within the semantic priming paradigm. Results highlighted key processing abnormalities in PD. Specifically, persons with PD exhibited a delayed time course of semantic activation. In addition, results suggest that experimental participants were unable to implicitly process prime information and, therefore, failed to engage strategic processing mechanisms in response to manipulations of the relatedness proportion. Results are discussed in terms of the Gain/Decay hypothesis (Milberg, McGlinchey-Berroth, Duncan, Higgins, 1999) and the dopaminergic modulation of signal to noise ratios in semantic networks.
Where are youth physically active? A descriptive examination of 45 parks in a southeastern community.

BACKGROUND The purpose of this study was to identify the activity settings used and physical activity (PA) intensity achieved by boys and girls in 45 parks in a southeastern community. METHODS PA and sedentary behaviors were assessed at all activity settings by gender, race, and PA intensity. Eight activity settings were identified in 45 parks. The System for Observing Play and Recreation in Communities (SOPARC) instrument was used to assess PA during the summers of 2004 through 2008. RESULTS More boys (n = 1,668; 58%) were observed at the 45 parks than girls (n = 1,184, 42%). Playgrounds were the most frequently used activity setting by both boys and girls. The vast majority of PA observed on the playgrounds was vigorous PA (n = 584; 41%). Playing fields were the second most frequently used activity setting by male and female youth, and 292 (10.3%) of all boys and girls were observed using this activity setting. However, only 18 of the 45 parks (40%) had a playing field. More males (n = 164; 56%) used the playing fields for PA than girls (n = 128; 44%). Most boys and girls were observed participating in vigorous PA (n = 254; 87%) at this activity setting. Observations of seven of the eight activity settings in the 45 parks indicated a greater frequency and percentage of white youth observed in comparison to minorities. CONCLUSIONS Identifying the patterns of children and teens in community parks will provide investigators with specific objective data to develop user profiles that can perhaps lead to effective PA interventions in these environmental settings.
The Role of Clostridium difficile and Viruses as Causes of Nosocomial Diarrhea in Children

Objective: We report surveillance of nosocomial diarrhea in children at our institution during the past decade and note different epidemiology of diarrhea due to viruses and Clostridium difficile. Design: A prospective cohort study. Setting: A university-affiliated pediatric hospital with 180 beds serving an urban area and providing referral care for the Maritime Provinces of Canada. Participants: Children younger than 18 years. Methods: Surveillance was conducted from 1991 to 1999 using personal contact with personnel and review of microbiology and medical records. Nosocomial diarrhea was defined as loose stools occurring more than 48 hours after admission, with at least two loose stools in 12 hours and no likely non-infectious cause. Results: Nosocomial diarrhea was the third most common nosocomial infection (217 of 1,466; 15%), after bloodstream and respiratory infections, with from 0.5 to 1 episode per 1,000 patient-days. Of 217 nosocomial diarrhea episodes, 122 (56%) had identified pathogens: C. difficile (39 of 122; 32%), rotavirus (38 of 122; 31%), adenovirus (36 of 122; 30%), and other viral (9 of 122; 7%). The median age was 1.3 years (range, 11 days to 17.9 years), 0.80 year for children with viral diarrhea, 3.9 years for children with C. difficile, and 1.5 years for children with diarrhea without a causative organism identified (P< .0001). Most children with nosocomial diarrhea were incontinent (diapered) at the time of their first episode (138 of 185; 75%), but preexisting incontinence was more common in those with viral diarrhea (93%) compared with those with no organism identified (71%) or those with C. difficile-associated diarrhea (CDAD) (49%) (P< .0001). Conclusions: C. difficile is the single most common cause of nosocomial diarrhea in our tertiary-care center, although all viral pathogens account for 69% of cases. Diapered status appears to be a risk factor for CDAD in children, and CDAD occurs more often in older children than viral nosocomial diarrhea. Further characterization of risk factors for, and morbidity associated with, nosocomial CDAD in children is warranted.
How Can Acute Mountain Sickness be Quantified at Moderate Altitude?

Reports of acute mountain sickness (AMS) at moderate altitude show a wide variability, possibly because of different investigation methods. The aim of our study was to investigate the impact of investigation methods on AMS incidence. Hackett's established AMS score (a structured interview and physical examination), the new Lake Louise AMS score (a self-reported questionnaire) and oxygen saturation were determined in 99 alpinists after ascent to 2.94 km altitude. AMS incidence was 8% in Hackett's AMS score and 25% in the Lake Louise AMS score. Oxygen saturation correlated inversely with Hackett's AMS score with no significant correlation with the Lake Louise AMS score. At moderate altitude, the new Lake Louise AMS score overestimates AMS incidence considerably. Hackett's AMS score remains the gold standard for evaluating AMS incidence.
Ultrastructural study by backscattered electron imaging and elemental microanalysis of bone-to-biomaterial interface and mineral degradation of porcine xenografts used in maxillary sinus floor elevation.

OBJECTIVES The aim of this study was to carry out an ultrastructural study of the biomaterial-to-bone interface and biomaterial mineral degradation in retrieved bone biopsies following maxillary sinus augmentation using collagenized porcine xenografts (Osteobiol(®) Mp3) in 15 clinical cases. MATERIALS AND METHODS Nine months after sinus lifting, bone cores were harvested from the maxillary sinus. The specimens were processed for observation under a scanning electron microscope with backscattered electron imaging (SEM-BSE). In addition, chemical analysis and elemental mapping of the mineral composition were generated using a microanalytical system based on energy-dispersive X-ray spectrometry (EDX). RESULTS No clinical complications were evident during surgery. Scanning electron microscopy revealed that newly formed bone had become closely attached to the xenograft. Statistical analysis showed a significantly high Ca/P ratio in the biomaterial (2.46 ± 0.16) and at the bone interface (2.00 ± 0.48) compared to bone (1.97 ± 0.36), which suggests that there may be a gradual diffusion of Ca ions from the biomaterial into the newly forming bone at the interface as part of the biomaterial's resorption process. EDX analysis of the residual porcine biomaterial at different points showed some particle categories with different mean ratios of Ca/P according to size, pointing to different stages of the resorption process. CONCLUSIONS The biomaterial proved to be biocompatible, bioreabsorbable and osteoconductive when used as a bone substitute for maxillary sinus elevation. SEM-BSE revealed that newly formed bone had become closely attached to the xenografts. EDX analysis monitored the resorption process of the porcine bone xenograft. Elemental mapping showed that there was a gradual diffusion of Ca ions from the biomaterial to the newly forming bone at the interface.
Effect of amitriptyline vs. physiotherapy in management of fibromyalgia syndrome: What predicts a clinical benefit?

CONTEXT Fibromyalgia is a chronic disabling condition, and physicians treat it using a number of different treatment modalities. It is not known if one or more of such modalities are better than the others. We compared the efficacy of physiotherapy and amitriptyline in disability reduction in patients of fibromyalgia syndrome in a rural tertiary care hospital in Central India. DESIGN Open-label alternate patient treatment allocation. MATERIALS AND METHODS A six-month follow-up was done to assess the benefit of amitriptyline and physiotherapy for disability reduction in patients with fibromyalgia syndrome. Primary outcome measure was improvement in fibromyalgia impact questionnaire (FIQ) score. Statistical Analysis Used : Predictors of benefit were determined using multivariate logistic regression. RESULTS A total of 175 outpatients were assigned to either amitriptyline (n=87) or structured physiotherapy (n=88) treatments. There was a significant but similar (P=0.82) improvement in disability in both groups. High FIQ score at baseline and low socioeconomic status scores were significant predictors of benefit. CONCLUSIONS Therapy with amitriptyline or physiotherapy is equally effective in improving outcome in patients of fibromyalgia over a period of six months.
DNA polymerase lambda (Pol lambda), a novel eukaryotic DNA polymerase with a potential role in meiosis.

A new gene (POLL) encoding a novel DNA polymerase (Pol lambda) has been identified at mouse chromosome 19. Murine Pol lambda, consisting of 573 amino acid residues, has a 32% identity to Pol beta, involved in nuclear DNA repair in eukaryotic cells. It is interesting that Pol lambda contains all the critical residues involved in DNA binding, nucleotide binding and selection, and catalysis of DNA polymerization, that are conserved in Pol beta and other DNA polymerases belonging to family X. Murine Pol lambda, overproduced in Escherichia coli, displayed intrinsic DNA polymerase activity when assessed by in situ gel analysis. Pol lambda also conserves the critical residues of Pol beta required for its intrinsic deoxyribose phosphate lyase (dRPase) activity. The first 230 amino acid residues of Pol lambda, that have no counterpart in Pol beta, contain a BRCT domain, present in a variety of cell-cycle check-point control proteins responsive to DNA damage and proteins involved in DNA repair. Northern blotting, in situ hybridization analysis and immunostaining showed high levels of Pol lambda specifically expressed in testis, being developmentally regulated and mainly associated to pachytene spermatocytes. These first evidences, although indirect, suggest a potential role of Pol lambda in DNA repair synthesis associated with meiosis.