Miracle-dz/UniFAR-FTUs huggingface dataset:
Morphological analysis of Trichomycterus areolatus Valenciennes, 1846 from southern Chilean rivers using a truss-based system (Siluriformes, Trichomycteridae)

Abstract Trichomycterus areolatus Valenciennes, 1846 is a small endemic catfish inhabiting the Andean river basins of Chile. In this study, the morphological variability of three T. areolatus populations, collected in two river basins from southern Chile, was assessed with multivariate analyses, including principal component analysis (PCA) and discriminant function analysis (DFA). It is hypothesized that populations must segregate morphologically from each other based on the river basin that they were sampled from, since each basin presents relatively particular hydrological characteristics. Significant morphological differences among the three populations were found with PCA (ANOSIM test, r = 0.552, p < 0.0001) and DFA (Wilks’s λ = 0.036, p < 0.01). PCA accounted for a total variation of 56.16% by the first two principal components. The first Principal Component (PC1) and PC2 explained 34.72 and 21.44% of the total variation, respectively. The scatter-plot of the first two discriminant functions (DF1 on DF2) also validated the existence of three different populations. In group classification using DFA, 93.3% of the specimens were correctly-classified into their original populations. Of the total of 22 transformed truss measurements, 17 exhibited highly significant (p < 0.01) differences among populations. The data support the existence of T. areolatus morphological variation across different rivers in southern Chile, likely reflecting the geographic isolation underlying population structure of the species.
Generation of functionally distinct B lymphocytes from common myeloid progenitors

Current models of adult haematopoiesis propose that haematopoietic stem cells (HSCs) differentiate into common lymphoid (CLP) and common myeloid (CMP) progenitors and establish an early separation between myeloid and lymphoid lineages. Nevertheless, the developmental potential of CMP‐associated B cells suggests the existence of alternate pathways for B lymphopoesis. The aim of this study was to compare the developmental and functional properties of CMP‐ and CLP‐derived B cells. While both populations matured through pro‐B cell and transitional B cell intermediates in the bone marrow and spleen, respectively, following transfer into irradiated mice, mature CMP‐ and CLP‐derived B cells exhibit distinct functional responses. Specifically, CMP‐derived B cells did not respond to mitogenic stimulation to the same degree as their CLP‐derived counterparts and secrete lower levels of IgM and the inflammatory cytokines such as interleukin (IL)‐6 and IL‐10. Together, these data suggest the existence of multiple pathways for generating functionally distinct B cells from bone marrow precursors.
A sensitive LC-MS/MS method for measurement of organophosphorus pesticides and their oxygen analogs in air sampling matrices

A rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed for determination of levels of the organophosphorus (OP) pesticides chlorpyrifos (CPF), azinphos methyl (AZM), and their oxygen analogs chlorpyrifos-oxon (CPF-O) and azinphos methyl-oxon (AZM-O) on common active air sampling matrices. XAD-2 resin and polyurethane foam (PUF) matrices were extracted with acetonitrile containing stable-isotope labeled internal standards (ISTD). Analysis was accomplished in Multiple Reaction Monitoring (MRM) mode, and analytes in unknown samples were identified by retention time (±0.1 min) and qualifier ratio (±30% absolute) as compared to the mean of calibrants. For all compounds, calibration linearity correlation coefficients were ≥0.996. Limits of detection (LOD) ranged from 0.15–1.1 ng/sample for CPF, CPF-O, AZM, and AZM-O on active sampling matrices. Spiked fortification recoveries were 78–113% from XAD-2 active air sampling tubes and 71–108% from PUF active air sampling tubes. Storage stability tests also yielded recoveries ranging from 74–94% after time periods ranging from 2–10 months. The results demonstrate that LC-MS/MS is a sensitive method for determining these compounds from two different matrices at the low concentrations that can result from spray drift and long range transport in non-target areas following agricultural applications. In an inter-laboratory comparison, the limit of quantification (LOQ) for LC-MS/MS was 100 times lower than a typical gas chromatography-mass spectrometry (GC-MS) method.
[Prognostic factors of functional recovery in very elderly stroke patients. A one-year follow-up study].

INTRODUCTION Although an advanced age is a factor associated to a poorer functional prognosis following a stroke, the capacity for recovery can be determined by other intercurrent clinical, functional and mental factors. AIM To evaluate the factors that determine the functional prognosis on discharge of very elderly patients who were admitted to hospital for their functional recovery after suffering a stroke. PATIENTS AND METHODS We conducted a longitudinal observational study of 168 patients over 65 years of age, who were hospitalised consecutively over a 15-month period. On admission, data concerning a number of clinical, neurological, functional and mental variables were collected. On being discharged from hospital their functional situation (Barthel index) and institutionalisation were evaluated. RESULTS The 48 patients aged 85 and above presented a lower degree of overall and relative functional recovery on discharge from hospital. Nevertheless, 52% had gained more than 20 points on the Barthel index on being discharged with respect to their score when they were admitted; on discharge 44% had recovered over 50% of the functional loss they had suffered following the stroke. On including the other basal variables in a logistic regression analysis, a very advanced age was associated in an independent manner to a greater risk of being institutionalised, but not to a poorer functional prognosis at discharge. Severe functional impairment on admission and post-stroke depression were the factors that were independently associated to moderate-severe disability on admission; in addition, the more severe the neurological consequences were, the lower the level of functional recovery was. CONCLUSIONS The capacity for functional recovery in very elderly stroke patients is mainly determined by the degree of functional and neurological repercussion. The functional prognosis should be individualised according to these factors, regardless of the age.
Phase error analysis for reflectarray antennas based on study of quasi-periodic effect

Reflectarray antennas can be considered as quasi-periodic structures in which similar elements locate on periodic grids. In this paper, we compare the reflection phase of elements in the array between the designed value and the practical one. This error is mainly due to the assumption of periodicity in computing the designed element reflection phase. To study this error, we developed a method to obtain the reflection phase of a unit-cell in a practical reflectarray. On this basis, a workflow to decrease the phase error is developed and validated on a 665-element reflectarray antenna. Over 1dB increase on the antenna gain and 8 percent increase on aperture efficiency are observed.
Karyotype analysis in lignosus bean ( Dipogon lignosus ) and lablab bean ( Lablab purpureus )

The experiment was conducted in the cytogenetics laboratory of the Department of Genetics and Plant Breeding of Bangladesh Agricultural University during July 2008 to September 2009 aiming to analyze and compare the karyotypes of two genotypes of Lignosus bean (Dipogon lignosus L.) and Lablab bean (Lablab purpureus L.). Root tips from germinated seeds were collected, pretreated and fixed in acetic alcohol for using in slide preparation. Chromosome measurements were done from the prints of the photomicrographs of prometaphase plates. In both the genotypes, 2n = 22 chromosomes was found. The range of length of the individual chromosome was 1.22 to 2. 17µ in Lignosus bean, and 1.17 to 3. 00µ in Lablab bean. In the Lignosus bean genotype, the haploid complement consisted of 11 metacentric (m) chromosomes with 3 individually identifiable ones but incase of Lablab bean the haploid complement consisted of 11 m chromosomes with 5 individually identifiable ones. All identifiable chromosomes were metacentric. This karyotype analysis of Lignosus bean and Lablab bean will be useful for further genetic studies.
Determinants of an impaired quality of life five years after coronary artery bypass surgery

OBJECTIVE To identify determinants of an inferior quality of life (QoL) five years after coronary artery bypass grafting (CABG). SETTING University hospital. PARTICIPANTS Patients from western Sweden who underwent CABG between 1988 and 1991. MAIN OUTCOME MEASURES Questionnaires for evaluating QoL before CABG and five years after operation. Three different instruments were used: the Nottingham health profile (NHP), the psychological general wellbeing index (PGWI), and the physical activity score (PAS). RESULTS 2121 patients underwent CABG, of whom 310 died during five years’ follow up. Information on QoL after five years was available in 1431 survivors (79%). There were three independent predictors for an inferior QoL with all three instruments: female sex, a history of diabetes mellitus, and a history of chronic obstructive pulmonary disease. Multivariate analysis showed that a poor preoperative QoL was a strong independent predictor for an impaired QoL five years after CABG. An impaired QoL was also predicted by previous disease. CONCLUSIONS Female sex, an impaired QoL before surgery, and other diseases such as diabetes mellitus are independent predictors for an impaired QoL after CABG in survivors five years after operation.
Horizontal cells in cat and monkey retina express different isoforms of glutamic acid decarboxylase

Abstract The neurotransmitter used by horizontal cells in mammals has not been identified. GABA has been the leading candidate, but doubt has remained because of failure to clearly demonstrate the GABA synthetic enzyme, glutamic acid decarboxylase (GAD) in these cells. Because GAD was recently shown to exist as two isoforms, 65 kDa and 67 kDa, we considered whether there might be a mismatch between the forms of GAD expressed in horizontal cells and the probes used to detect it. Accordingly, we stained sections of mammalian retina with antibodies specific for each isoform. Cat horizontal cells of both types (A and B) were immunoreactive for GAD67 but negative for GAD65; monkey horizontal cells of both types (H1 and H11) were positive for GAD65 and negative for GAD67. The findings reconcile previous, apparently conflicting, observations and strengthen considerably the hypothesis that mammalian horizontal cells are GABAergic.
Fine-scale degrader community profiling over an aerobic/anaerobic redox gradient in a toluene-contaminated aquifer.

Hydrocarbon contaminants in groundwater can be degraded by microbes under different redox settings, forming hot spots of degradation especially at the fringes of contaminant plumes. At a tar-oil-contaminated aquifer in Germany, it was previously shown that the distribution of anaerobic toluene degraders as traced via catabolic and ribosomal marker genes is highly correlated to zones of increased anaerobic degradation at the lower fringe of the plume. Here, we trace the respective distribution of aerobic toluene degraders over a fine-scale depth transect of sediments taken at the upper fringe of the plume and below, based on the analysis of 16S rRNA genes as well as catabolic markers in intervals of 3-10 cm. Well-defined small-scale distribution maxima of typical aerobic degrader lineages within the Pseudomonadaceae, Comamonadaceae and Burkholderiaceae are revealed over the redox gradient. An unexpected maximal abundance of 9.2 × 10⁶ toluene monooxygenase (tmoA) genes per g of sediment was detected in the strongly reduced plume core, and gene counts did not increase towards the more oxidized upper plume fringe. This may point towards unusual ecological controls of these yet unidentified aerobic degraders, and indicates that competitive niche partitioning between aerobic and anaerobic hydrocarbon degraders in the field is not yet fully understood. These findings demonstrate the potential of catabolic marker gene assays in elaborating the ecology of contaminant plumes, which is a prerequisite for developing integrated monitoring strategies for natural attenuation.
Novel Hot-Carrier Degradation Mechanisms in the Lateral Insulated-Gate Bipolar Transistor on SOI Substrate

The different hot-carrier degradation mechanisms of the lateral insulated-gate bipolar transistor on a silicon-on-insulator substrate (SOI-LIGBT) for different stress conditions have been experimentally investigated for the first time. For low Vgs and high Vds, the hot hole injects and traps into the accumulation and the field oxide, particularly the bird's beak, which results in the decrease in the on-resistance Ron at the early stress stage. It is interesting that the decrease level of Ron in SOI-LIGBT is much more serious than that in the SOI laterally diffused metal-oxide-semiconductor with the same structure fully except for the doping type in the drain area. In addition, the buried oxide surface under the drain area also suffers from severe hot-carrier degradation. However, for high Vgs and low Vds, only hot-electron injection into the gate oxide near the source side can be observed; there is no hot-carrier degradation to be found in both the field and buried oxides.
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Accuracy of Endoscopic Ultrasonography and Magnetic Resonance Cholangiopancreatography for the Diagnosis of Chronic Pancreatitis: A Prospective Comparison Study

Background The diagnosis of chronic pancreatitis (CP) remains challenging. Endoscopic ultrasound (EUS) and magnetic resonance cholangiopancreatography (MRCP) have been proposed as highly accurate diagnostic modalities. Although endoscopic retrograde cholangiopancreatography (ERCP) has been previously used as a gold standard, it is associated with a small but significant risk. We aim to compare the accuracy of EUS and MRCP with the composite gold standard using ERCP, surgical pathology, and/or long-term clinical follow-up. Methods Ninety-nine patients with clinical signs and/or symptoms suggestive of CP were prospectively enrolled. The diagnosis of CP by MRCP was established when one or more of these features were present: main duct dilation without obstruction, dilated side branches, intraductal stones, ductal irregularity, reduced T1-signal intensity, parenchymal atrophy, and reduced secretory response to secretin administration. The diagnosis of CP by EUS was made when 4 or more of the established criteria were present. Accuracy of all criteria used (“EUS only,” “MRCP only,” “either EUS or MRCP,” and “both EUS and MRCP”) was compared with the composite gold standard. Results Forty patients were diagnosed with CP by the composite gold standard whereas the remaining 59 patients were controls. EUS only seemed more sensitive but equally specific compared with MRCP only to diagnose CP. The combination of EUS and MRCP had a sensitivity of 98% for either EUS or MRCP and a specificity of 100% for both EUS and MRCP. Conclusions EUS and MRCP are highly accurate modalities for the diagnosis of CP and are complementary when used together. If confirmed in larger series, the diagnosis of CP by both EUS and MRCP is highly predictive and ERCP is unlikely to add any useful information.
L'italiano al telefonino: Using SMS to support beginners' language learning1

Abstract This article discusses an experiment in sending regular Short Message Service (SMS) messages to support language learning, and vocabulary learning in particular, at beginners' level in Italian at an Australian university. The approach we took built on the initiatives of Thornton and Houser (2005) and Dias (2002b), and was informed by the results of an earlier trial we had conducted with students at high-intermediate level (Levy & Kennedy, 2005). In testing the possibilities for using mobile phones for language learning purposes, we were especially interested in investigating the acceptability of a ‘push’ mode of operation, in which the scheduling of messages is determined by the teachers. While the students appreciated the experience overall, and found the message content often useful or enjoyable, there was a wide range of views on the frequency of messages acceptable. We are therefore planning the further integration of messaging into the course around a flexible arrangement involving options for high or low frequency of pushed messages, as well as messages available on request – in ‘pull’ mode.
A genomewide screen for components of the RNAi pathway in Drosophila cultured cells

Posttranscriptional silencing by RNAi is initiated by dsRNAs that are processed into siRNAs that ultimately target homologous mRNAs for degradation. We used luciferase reporter constructs and a cultured cell-based assay to perform a genomewide screen for components of the RNAi pathway in Drosophila melanogaster. The screen identified seven genes that affect the RNAi response, five with previously described function (AGO2, Tis11, Hsc70-3, Hsc70-4, and hdc) and two annotated genes (CG17265 and CG10883).
Moment arms of the shoulder musculature after reverse total shoulder arthroplasty.

BACKGROUND Reverse total shoulder arthroplasty is known to increase the moment arm of the middle subregion of the deltoid during shoulder abduction; however, at present, comprehensive data regarding the shoulder muscle moment arm through the full range of abduction and flexion are not available. The purpose of this study was twofold: (1) to measure the instantaneous moment arms of thirteen subregions of major muscles spanning the glenohumeral joint during abduction and flexion of the shoulder after reverse total shoulder arthroplasty and (2) to compare these data with the muscle moment arms previously measured preoperatively in the anatomical shoulders. METHODS Reverse total shoulder arthroplasty was performed on eight entire cadaveric upper extremities. The specimens were mounted onto a dynamic testing apparatus, and the instantaneous abductor/adductor and flexor/extensor moment arms of subregions of the deltoid, latissimus dorsi, pectoralis major, teres major, and subscapularis muscles (a total of thirteen subregions) were measured with use of the tendon excursion method. These muscle moment arms were compared with those measured preoperatively in the anatomical shoulders. RESULTS Reverse total shoulder arthroplasty resulted in significant increases in the abductor moment arms of the anterior subregion of the deltoid (mean increase = 10.4 mm; 95% confidence interval = 7.5 to 13.3 mm) and the middle subregion of the deltoid (mean increase = 15.5 mm; 95% confidence interval = 10.8 to 20.3 mm) as well as recruitment of the posterior subregion of the deltoid as an abductor. The superior subregion of the pectoralis major (the clavicular fibers) and anterior subregion of the deltoid were the most effective flexors and had a substantial potential to initiate flexion. The adductor and extensor moment arms of the teres major, latissimus dorsi subregions, and inferior and middle subregions of the pectoralis major increased substantially after the arthroplasty. The subscapularis subregions behaved as extensors, abductors, and adductors after the arthroplasty; this was in contrast to their roles in the anatomical shoulder, in which they were mainly flexors and adductors. CONCLUSIONS Reverse total shoulder arthroplasty increases the moment arms of the major abductors, flexors, adductors, and extensors of the glenohumeral joint, thereby reducing muscle effort during common tasks such as lifting and pushing.
Interaction of the Molecular Chaperone HtpG with Uroporphyrinogen Decarboxylase in the Cyanobacterium Synechococcus elongatus PCC 7942

Uroporphyrinogen decarboxylase (HemE) is important due to its location at the first branch-point in tetrapyrrole biosynthesis. We detected a complex formation between full-length polypeptides of HtpG and HemE by biochemical studies in vivo and in vitro. The interaction suppressed the enzyme activity, suggesting a regulatory role of HtpG in tetrapyrrole biosynthesis.
Cysteine Scanning Mutagenesis of the Segment between Putative Transmembrane Helices IV and V of the High Affinity Na+/Glucose Cotransporter SGLT1

Site-directed mutagenesis and chemical modification of specific cysteine amino acid side chains by methanethiosulfonate (MTS) derivatives were combined to elucidate structure/function relationships of the cloned rabbit Na+/glucose cotransporter, SGLT1. Each amino acid in the region (residues 162–173) between putative transmembrane helices IV and V of SGLT1 was replaced individually with Cys. Mutant proteins were expressed in Xenopus laevis oocytes and studied using the two-electrode voltage clamp method. At certain key positions, Cys substitution resulted in 1) a change in the apparent affinity for sugar, 2) an alteration in the voltage dependence of the transient currents, and 3) a sensitivity to inhibition by either the ethylamine (MTSEA) or the ethylsulfonate MTS derivatives. For the three Cys mutants inhibited by MTSEA (F163C, A166C, and L173C), inhibition of steady state transport is related to changes in membrane potential-dependent transitions within the Na+/glucose transport cycle. MTSEA shifted the transient currents of these Cys mutants toward more negative membrane potentials (ΔV 0.5 = −18 mV for F163C and A166C, −12 mV for L173C). When the mutations were combined to produce double and triple Cys mutants, the degree to which the transient currents were shifted along the membrane potential axis by MTSEA correlated with the number of cysteines. In this way it was possible to manipulate the voltage dependence of the transient currents over a range spanning 91 mV. Examination of the Na+ dependence of the transient currents indicates that a 91-mV shift is equivalent to that caused by a 10-fold reduction in the external Na+ concentration. We conclude that this region has a role in determining the Na+binding- and voltage-sensing properties of SGLT1 and that it forms an α-helix with one surface possibly lining a Na+ pore within SGLT1.