Miracle-dz/UniFAR-FTUs huggingface dataset:
A high‐resolution all‐digital pulse‐width modulator architecture with a tunable delay element in CMOS

A design for an all‐digital high‐resolution pulse‐width modulator (HRPWM) architecture is presented in this work. The architecture is based on a novel digitally controlled delay element that combines two different approaches, achieving a variable time interval up to 54 ps, and adjustable against process, voltage, and temperature (PVT) variations. The proposed system uses several delay elements with a counter‐based digital pulse‐width modulator (DPWM) in a hybrid configuration, which allows to obtain duty cycles with 18‐bit resolution without using a high‐frequency internal clock and maintaining a low power dissipation.
Ziv-aflibercept and bevacizumab for exudative age-related macular degeneration: A retrospective comparison of clinical outcomes and cost at 1 year

PURPOSE: The purpose of this study was to compare intravitreal ziv-aflibercept (IVZ) monotherapy to intravitreal bevacizumab (IVB) monotherapy in patients with exudative age-related macular degeneration (eAMD). MATERIALS AND METHODS: Patients with treatment-naïve eAMD treated with pro re nata (PRN) monotherapy of IVZ (1.25 mg/0.05 ml) or IVB (1.25 mg/0.05 ml) with a minimum follow-up of 12 months were retrospectively analyzed. Study outcomes included change in best-corrected visual acuity (BCVA), central macular thickness, mean number of injections, and total medication cost in both the groups at 12 months. RESULTS: Forty-seven eyes (IVZ, 18/47 [38.3%] and IVB, 29/47 [61.7%]) from 47 treatment-naive patients were included. The change in BCVA for patients receiving IVZ was from 0.61 ± 0.33 logarithm of the minimum angle of resolution (Snellen 20/81; range: 20/38–20/174) to 0.45 ± 0.31 (Snellen 20/56; range: 20/27–20/115) at 1 year (P = 0.02). The total number of injections needed to achieve the resolution of intraretinal or subretinal fluid was 2.6 ± 1.4 and 3.5 ± 1.3 for IVZ and IVB, respectively (P = 0.029). Direct medication cost of IVZ and IVB in our cohort on PRN basis was an average of US$78 (2.6 × US$30) and US$175 (3.5 × US$50), respectively, through 1 year. CONCLUSION: IVZ-PRN monotherapy resulted in improved visual acuity, reduced treatment burden, and reduced direct medication cost in comparison to IVB-PRN monotherapy through 1 year.
Neuromuscular and postural control in visually and nonvisually impaired judo athletes: case study

This study aimed to analyze neuromuscular and postural control in visually and nonvisually impaired judo athletes. Two judo athletes, one visually impaired and the other nonvisually impaired, participated in the study. The athletes presented similar demographic, anthropometric, and judo-technical characteristics. They performed maximal isometric handgrip strength (dominant and nondominant hand), vertical jumps (countermovement jump [CMJ] and squat jump [SJ]), and center of pressure assessment in three positions: neutral, anteroposterior, and judo combat base (Migi-shizentai). The main findings showed that the visually impaired athlete presented higher standing balance in the neutral and anteroposterior positions than non-visually impaired athlete (effect size [ES]>2.0). In the Migi-shizentai position, the disparity between both athletes was reduced, particularly in the displacement area (ES=0.52). The visually impaired athlete showed higher performance in the SJ but lesser performance in CMJ and handgrip strength tests than nonimpaired athlete (ES>2.0). We concluded that the postural stability was higher in the visually impaired athlete in the neutral and anteroposterior position, but similar to the nonvisually impaired athlete in Migi-shizentai position, possible due to the influence of judo practice. Moreover, the visually impaired athlete showed higher performance in the SJ than nonvisually impaired.
Class imbalance learning–driven Alzheimer’s detection using hybrid features

Alzheimer’s is the main reason which leads to memory loss of a human being. The living style of an affected person also varies. This variation in the lifestyle creates difficulties for a person to spend a normal life. The detection of Alzheimer’s disease in the initial stage accurately has a great significance in the early treatment of the disease. Therefore, it is considered as a challenging task. An efficient method is proposed to detect the Alzheimer’s disease in the early stage using magnetic resonance imaging. k-Means clustering is used to develop the proposed method for efficient segmentation of the white matter, cerebrospinal fluid, and grey matter. The amalgam feature vectors are formed using the grey-level co-occurrence matrix and speeded-up robust features based on textural feature extraction. Statistical and histogram of gradients is used from shape-based features. Fisher linear discriminant analysis is used for dimensionality reduction and the resampling method is used to handle the class imbalance problem. The algorithm is evaluated using the three classifiers k-nearest neighbour, support vector machine and random forest. The dataset of OASIS is used to assess the outcomes. The proposed approach achieved the optimum accuracy of 92.7%.
The Fracture Behavior and Mechanical Properties of a Support Structure for Additive Manufacturing of Ti-6Al-4V

In the laser powder bed fusion processes for metal additive manufacturing, a support structure is needed to fix the part to the base plate and to support overhanging regions. Currently the importance of support structure for a successful build process is often underestimated and some effects are not yet well understood. Therefore, this study investigates the fracture behavior and mechanical properties of thin additive manufactured struts using the titanium alloy Ti-6Al-4V and specific machine parameters for support structures. Tensile tests were performed for different strut diameters and the fracture surfaces were analyzed using a laser microscope and a scanning electron microscope. Additionally, the porosity was examined with micro-CT scans. The results were compared with a different set of parameters used for solid parts. The experiments revealed that struts produced with support parameters had no significantly lower tensile strength than the comparative parts. Despite that, some porosity and around two percent of defects on the fracture surface for parts using the solid parameter set have been found. Parts with support parameters show no porosity, even though the energy density is around 30% lower compared to the solid parameter set.
ORB-SLAM, IMU and Wheel Odometry Fusion for Indoor Mobile Robot Localization and Navigation

In this paper, we propose a light-weight multi-sensor fusion method containing ORB-SLAM, IMU and wheel odometry for localization and navigation of an indoor mobile robot in GPS-denied environment. Known as an accepted generally visual simultaneous localization and mapping (SLAM) system, ORB-SLAM based on feature matching computes real-time camera pose. The Inertial Measurement Unit (IMU) measures the angular velocity of the robot by one of its gyroscopes. The wheel odometry provides linear motion velocity for the robot and records distance the robot has moved. Through leveraging both rotation characteristic of IMU and linear characteristic of wheel odometry, the rough localization estimation for the robot is obtained. During every navigation of the robot, the rough localization estimation provides relatively accurate mapping scale of the real world for ORB-SLAM. And the mapping scale revises the monocular camera pose of ORB-SLAM to obtain global robot pose estimation in the real world. In the experiment, the robot can locate itself with tolerable error and perform great navigation ability in a specific scene.
Teaching patient safety in global health: lessons from the Duke Global Health Patient Safety Fellowship

Health systems in low-income and middle-income countries (LMICs) have a high burden of medical errors and complications, and the training of local experts in patient safety is critical to improve the quality of global healthcare. This analysis explores our experience with the Duke Global Health Patient Safety Fellowship, which is designed to train clinicians from LMICs in patient safety, quality improvement and infection control. This intensive fellowship of 3–4 weeks includes (1) didactic training in patient safety and quality improvement, (2) experiential training in patient safety operations, and (3) mentorship of fellows in their home institution as they lead local safety programmes. We have learnt several lessons from this programme, including the need to contextualise training to local needs and resources, and to focus training on building interdisciplinary patient safety teams. Implementation challenges include a lack of resources and data collection systems, and limited recognition of the role of safety in global health contexts. This report can serve as an operational guide for intensive training in patient safety that is contextualised to global health challenges.
Analysis of Transcriptome and Epitranscriptome in Plants Using PacBio Iso-Seq and Nanopore-Based Direct RNA Sequencing

Nanopore sequencing from Oxford Nanopore Technologies (ONT) and Pacific BioSciences (PacBio) single-molecule real-time (SMRT) long-read isoform sequencing (Iso-Seq) are revolutionizing the way transcriptomes are analyzed. These methods offer many advantages over most widely used high-throughput short-read RNA sequencing (RNA-Seq) approaches and allow a comprehensive analysis of transcriptomes in identifying full-length splice isoforms and several other post-transcriptional events. In addition, direct RNA-Seq provides valuable information about RNA modifications, which are lost during the PCR amplification step in other methods. Here, we present a comprehensive summary of important applications of these technologies in plants, including identification of complex alternative splicing (AS), full-length splice variants, fusion transcripts, and alternative polyadenylation (APA) events. Furthermore, we discuss the impact of the newly developed nanopore direct RNA-Seq in advancing epitranscriptome research in plants. Additionally, we summarize computational tools for identifying and quantifying full-length isoforms and other co/post-transcriptional events and discussed some of the limitations with these methods. Sequencing of transcriptomes using these new single-molecule long-read methods will unravel many aspects of transcriptome complexity in unprecedented ways as compared to previous short-read sequencing approaches. Analysis of plant transcriptomes with these new powerful methods that require minimum sample processing is likely to become the norm and is expected to uncover novel co/post-transcriptional gene regulatory mechanisms that control biological outcomes during plant development and in response to various stresses.
Sampling and Processing Methods Impact Microbial Community Structure and Potential Activity in a Seasonally Anoxic Fjord: Saanich Inlet, British Columbia

The Scientific Committee on Oceanographic Research (SCOR) Working Group 144 Microbial Community Responses to Ocean Deoxygenation workshop held in Vancouver, B.C on July 2014 had the primary objective of initiating a process to standardize operating procedures for compatible process rate and multi-omic (DNA, RNA, protein and metabolite) data collection in marine oxygen minimum zones (OMZs) and other oxygen depleted waters. Workshop attendees participated in practical sampling and experimental activities in Saanich Inlet, British Columbia, a seasonally anoxic fjord. Experiments were designed to compare and cross-calibrate in situ versus bottle sampling methods to determine effects on microbial community structure and potential activity when using different filter combinations, filtration methods and sample volumes. Resulting biomass was preserved for small subunit ribosomal RNA (SSU or 16S rRNA) and SSU rRNA gene (rDNA) amplicon sequencing followed by downstream statistical and visual analyses. Results from these analyses showed that significant community shifts occurred between in situ versus on ship processed samples. For example, Bacteroidetes, Alphaproteobacteria and Opisthokonta associated with on-ship filtration onto 0.4 µm filters increased 5-fold compared to on-ship in-line 0.22 µm filters or 0.4 µm filters processed and preserved in situ. In contrast, Planctomycetes associated with 0.4 µm in situ filters increased 5-fold compared to on-ship filtration onto 0.4 µm filters and on-ship in-line 0.22 µm filters. In addition, candidate divisions and Chloroflexi were primarily recovered when filtered onto 0.4 µm filters in situ. Results based on rRNA:rDNA ratios for microbial indicator groups revealed previously unrecognized roles of candidate divisions, Desulfarculales, and Desulfuromandales in sulfur cycling, carbon fixation and fermentation within anoxic basin waters. Taken together, filter size and in situ versus on-ship filtration had the largest impact on recovery of microbial groups with the potential to influence downstream metabolic reconstruction and process rate measurements. These observations highlight the need for establishing standardized and reproducible techniques that facilitate cross-scale comparisons and more accurately assess in situ activities of microbial communities.
Aberrant Phase Transitions: Side Effects and Novel Therapeutic Strategies in Human Disease

Phase separation is a physiological process occurring spontaneously when single-phase molecular complexes separate in two phases, a concentrated phase and a more diluted one. Eukaryotic cells employ phase transition strategies to promote the formation of intracellular territories not delimited by membranes with increased local RNA concentration, such as nucleolus, paraspeckles, P granules, Cajal bodies, P-bodies, and stress granules. These organelles contain both proteins and coding and non-coding RNAs and play important roles in different steps of the regulation of gene expression and in cellular signaling. Recently, it has been shown that most human RNA-binding proteins (RBPs) contain at least one low-complexity domain, called prion-like domain (PrLD), because proteins harboring them display aggregation properties like prion proteins. PrLDs support RBP function and contribute to liquid–liquid phase transitions that drive ribonucleoprotein granule assembly, but also render RBPs prone to misfolding by promoting the formation of pathological aggregates that lead to toxicity in specific cell types. Protein–protein and protein-RNA interactions within the separated phase can enhance the transition of RBPs into solid aberrant aggregates, thus causing diseases. In this review, we highlight the role of phase transition in human disease such as amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and in cancer. Moreover, we discuss novel therapeutic strategies focused to control phase transitions by preventing the conversion into aberrant aggregates. In this regard, the stimulation of chaperone machinery to disassemble membrane-less organelles, the induction of pathways that could inhibit aberrant phase separation, and the development of antisense oligonucleotides (ASOs) to knockdown RNAs could be evaluated as novel therapeutic strategies for the treatment of those human diseases characterized by aberrant phase transition aggregates.
Potential of Incorporation of Antimicrobial Plant Phenolics Into Polyolefin-Based Food Contact Materials to Produce Active Packaging by Melt-Blending: Proof of Concept With Isobutyl-4-Hydroxybenzoate

There is an increasing interest for active food packaging incorporated with natural antimicrobial agents rather than synthetic preservatives. However, most of plastics for direct contact with food are made of polyolefins, usually processed by extrusion, injection, or blow-molding methods while most of natural antimicrobial molecules are thermolabile compounds (e.g., essential oils). Therefore, addition of plant phenolics (with low volatility) to different polyolefins might be promising to design active controlled release packaging processed by usual plastic compounding and used for direct contact with food products. Therefore, up to 2% (wt/wt) of isobutyl-4-hydroxybenzoate (IBHB) was mixed with 3 polyolefins: EVA poly(ethylene-co-vinyl acetate), LLDPE (Linear Low Density Polyethylene), and PP (PolyPropylene) by melt-blending from 75 to 170°C and then pelletized in order to prepare heat-pressed films. IBHB was chosen as an antibacterial phenolic active model molecule against Staphylococcus aureus to challenge the entire processing. Antibacterial activity of films against S. aureus (procedure adapted from ISO 22196 standard) were 4, 6, and 1 decimal reductions in 24 h for EVA, LLDPE, and PP films, respectively, demonstrating the preservation of the antibacterial activity after melt processing. For food contact materials, the efficacy of antimicrobial packaging depends on the release of the antimicrobial molecules. Therefore, the three types of films were placed at 23°C in 95% (v/v) ethanol and the release rates of IBHB were monitored: 101 ± 1%, 32 ± 7%, and 72 ± 9% at apparent equilibrium for EVA, LLDPE, and PP films, respectively. The apparent diffusion coefficients of IBHB in EVA and PP films were 2.8 ± 0.3 × 10−12 and 4.0 ± 1.0 × 10−16 m2s−1. For LLDPE films, IBHB crystals were observed on the surface of films by SEM (Scanning Electron Microscopy): this blooming effect was due the partial incompatibility of IBHB in LLDPE and its fast diffusion out of the polymer matrix onto the film surface. In conclusion, none of these three materials was suitable for a relevant controlled release packaging targeting the preservation of fresh food, but a combination of two of them is promising by the design of a multilayer packaging: the release could result from permeation through an inner PE layer combined with an EVA one acting as a reservoir.
Kinect-Assisted Performance-Sensitive Upper Limb Exercise Platform for Post-stroke Survivors

One's ability to use upper limbs is critical for performing activities of daily living necessary for enjoying quality community life. However, after stroke, such abilities becomes adversely affected and it often deprives one of their capability to perform tasks that need coordinated movement in the upper limbs. To address issues with upper limb dysfunction, patients typically undergo rehabilitative exercises. Given the high patient to doctor ratio particularly in developing countries like India, conventional rehabilitation with patients undergoing exercises under one-on-one therapist's supervision often becomes a challenge. Thus, investigators are exploring technology such as computer-based platforms coupled with cameras that can alleviate the need for the continuous presence of a therapist and can offer a powerful complementary tool in the hands of the clinicians. Such marker-based imaging systems used for rehabilitation can offer real-time processing and high accuracy of data. However, these systems often require dedicated lab space and high set-up time. Often this is very expensive and suffers from portability issues. Investigators have been exploring marker-less imaging techniques e.g., Kinect integrated computer-based graphical user interfaces in stroke-rehabilitation such as tracking one's limb movement during rehabilitation. In our present study, we have developed a Kinect-assisted computer-based system that offered Human Computer Interaction (HCI) tasks of varying challenge levels. Execution of the tasks required one to use reaching and coordination skills of the upper limbs. Also, the system was Performance-sensitive i.e., adaptive to the individualized residual movement ability of one's upper limb quantified in terms of task performance score. We tested for the usability of our system by exposing 15 healthy participants to our system. Subsequently, seven post-stroke patients interacted with our system over a few sessions spread over 2 weeks. Also, we studied patient's mean tonic activity corresponding to the HCI tasks as a possible indicator of one's post-stroke functional recovery suggesting its potential of our system to serve as a rehabilitation platform. Our results indicate the potential of such systems toward the improvement of task performance capability of post-stroke patients with possibilities of upper limb movement rehabilitation.
Recent progress in the design and synthesis of nanofibers with diverse synthetic methodologies: characterization and potential applications

Over the past two decades, the advancements of nanotechnology, particularly nanomaterials science, have produced a broad range of nanomaterials (NMs) that include nanofibers (NFs), nanoparticles (NPs), nanorods (NRs), and nanowires (NWs), which have been technically and practically examined over various applications. NFs have shown excellent potential for diverse applications in materials science, chemical industry, energy storage, and structural support due to a combination of remarkable properties such as high surface area, great electrical conductivity, and high mechanical stability. To date, a large number of mechanical and chemical synthetic methodologies (i.e., thermal-phase separation, electrospinning, pulling, self-assembly, and template processes) have been proposed to fabricate a new generation of NFs. In order to improve the performance of NFs, several functionalization steps have been proposed. The functionalization of NFs plays a prominent role in commercializing them on the industrial scale. In the current review article, recent progress in the fabrication and characterization of NFs is highlighted and reviewed to provide comprehensive methods on synthesizing a new generation of well-modified NFs with superior structural, physicochemical, and textural characteristics. In addition, the performance of synthesized NFs in a variety of practical applications, such as electrodes for fuel cells and lithium batteries, water and air purification, biomedicine, and catalysis, is reviewed.