Miracle-dz/UniFAR-FTUs huggingface dataset:
Antecedent of Knowledge Sharing Intentions: An Exploratory Study

In today’s globalized world, knowledge management has become an indispensable part of the functioning of any organization. Individuals tend to hold knowledge for various reasons, personality being one of the most important factors. This research aims to study the role of personality in knowledge-sharing behavior with emphasis on management students who are the future managers. Management institutes, being mini laboratories providing experiential learning to their students, have assimilated knowledge creation, acquisition and sharing as part of their continuous learning process. Knowledge management models have now become a part of the curricula. Big-Five Taxonomy, that include extraversion, neuroticism, openness to experience, agreeableness and conscientiousness, is considered as one the most stable taxonomies in classifying personality traits and has, thus, been used in the study. The study aims to understand the role of Big Five Personality traits in knowledge sharing behaviour in the context of management institutes. Survey data were collected from 352 management students from Delhi NCR. The results indicate that extraversion, agreeableness and openness to experience positively affect the knowledge sharing intention. The findings provide several important theoretical and practical implications regarding the role of personality on the knowledge-sharing intentions in the context of management education. The implications of these findings and suggestions for future research are also discussed in the paper.
Expression of transient receptor potential channel melastin (TRPM) 1–8 and TRPA1 (ankyrin) in mouse inner ear

Conclusions: It has been shown that TRPMs may play a functional role in sensory cell physiology, fluid homeostasis, sensory cell death, and thermosensation in the inner ear, while TRPA1 plays an important role in sensory transduction. Objective: To study expression of TRPM1–8 and TRPA1 in the mouse inner ear. Materials and methods: The localization of TRPM1–8 and TRPA1 in the inner ear of normal and gentamicin-treated CBA/J mice was investigated by immunohistochemistry. Results: The stria vascularis displayed a positive immunofluorescent reaction to TRPM1, 2, 3, 6, and 7. In the organ of Corti, outer and inner hair cells (OHCs and IHCs) showed positive immunofluorescence to TRPM1, 2, 3, 6, 7, and 8. Spiral ganglion cells were immunoreactive to TRPM1, 2, 3, 6, 7, and 8. The nerve fibers in the spiral ganglion cells and the nerves innervating the OHCs or IHCs were noticeably immunofluorescent to TRPM8 and TRPA1. In the vestibular end organs, vestibular sensory cells showed immunofluorescence to TRPM1, 2, 3, 6, and 7. The vestibular dark cells showed immunofluorescence to TRPM1, 3, 6, and 7; only the apical portion reacted to TRPM4. The nerve fibers innervating the vestibular sensory cells were distinctly reactive to TRPM8 and TRPA1, while the vestibular ganglion cells reacted to TRPM1, 2, 3, 6, 7, and 8.
Function and Dysfunction of Microglia during Brain Development: Consequences for Synapses and Neural Circuits

Many diverse factors, ranging from stress to infections, can perturb brain homeostasis and alter the physiological activity of microglia, the immune cells of the central nervous system. Microglia play critical roles in the process of synaptic maturation and brain wiring during development. Any perturbation affecting microglial physiological function during critical developmental periods could result in defective maturation of synaptic circuits. In this review, we critically appraise the recent literature on the alterations of microglial activity induced by environmental and genetic factors occurring at pre- and early post-natal stages. Furthermore, we discuss the long-lasting consequences of early-life microglial perturbation on synaptic function and on vulnerability to neurodevelopmental and psychiatric disorders.
Fe(III) Oxide Reduction by Anaerobic Biofilm Formation-DeficientS-Ribosylhomocysteine Lyase (LuxS) Mutant of Shewanella oneidensis

Abstract Shewanella oneidensis respires a variety of terminal electron acceptors, including solid phase Fe(III) oxides. S. oneidensis transfers electrons to Fe(III) oxides via direct (outer membrane- or nanowire-localized c-type cytochromes) and indirect (electron shuttling and Fe(III) solubilization) pathways. In the present study, the influence of anaerobic biofilm formation on Fe(III) oxide reduction by S. oneidensis was determined. The gene encoding the activated methyl cycle (AMC) enzyme S-ribosylhomocysteine lyase (LuxS) was deleted in-frame to generate the corresponding mutant ΔluxS. Conventional biofilm assays and visual inspection via confocal laser scanning microscopy indicated that the wild-type strain formed anaerobic biofilms on Fe(III) oxide-coated silica surfaces, while the ΔluxS mutant was severely impaired in anaerobic biofilm formation on such surfaces. Cell-hematite attachment isotherms demonstrated that the ΔluxS mutant was also severely impaired in attachment to hematite surfaces under anaerobic conditions. The S. oneidensis ΔluxS mutant, however, reduced Fe(III) at wild-type rates during anaerobic incubation with Fe(III) oxide-coated silica surfaces or in batch cultures with Fe(III) oxide or hematite as a terminal electron acceptor. Anaerobic biofilm formation by the ΔluxS mutant was restored to wild-type rates by providing a wild-type copy of luxS in trans or by the addition of AMC or transsulfurylation pathway metabolites involved in organic sulfur metabolism. LuxS is thus required for wild-type anaerobic biofilm formation on Fe(III) oxide surfaces, yet the inability to form wild-type anaerobic biofilms on Fe(III) oxide surfaces does not alter Fe(III) oxide reduction activity.
Attempted suicide related posttraumatic stress disorder in depression - an exploratory study

Studies evaluating stress hormone regulation after a suicide attempt reported an endocrine pattern similar to Post Traumatic Stress Disorder (PTSD). These findings led to the assumption that an attempted suicide may trigger the development of PTSD-like symptoms. To test this hypothesis, we retrospectively evaluated the incidence of PTSD in response to an attempted suicide in depressed patients participating in the Munich Antidepressant Response Signature (MARS) project. 30 patients with a history of a suicide attempt were included. 46.7% of these patients reported PTSD-like symptoms in specifically in response to the suicide attempt and independent from other past traumatic experiences. The risk to develop PTSD after a suicide attempt increased with the severity of the attempted suicide and with the precautions taken to prevent discovery. Our findings suggest that depressed patients surviving a suicide attempt experience a severe trauma likely to induce PTSD. We conclude that PTSD-specific medical and psychological interventions should be considered after severe suicide attempts. Keywords: Posttraumatic Stress Disorder, Acute Stress Reaction, Suicide Ideas, Suicide Attempts Copyrights belong to the Author(s). Suicidology Online (SOL) is a peer-reviewed open-access journal publishing under the Creative Commons License 3.0. Language: en
Maglev haptics: butterfly haptic's new user interface technology

There is an increasing interest in haptic (sense of touch) interaction with computers. A new kind of haptic system has recently been developed at Carnegie Mellon University and commercialized by Butterfly Haptics. The system eliminates the bulky links, cables and general mechanical complexity of other haptic devices on the market today in favor of a single lightweight moving part that floats on magnetic fields.
The biomechanics of walking symmetry during gait cycle in various walking condition

In the previous studies, contradictory results were reported on walking symmetry. The walking symmetry is important in the study of the evolution of bipedalism and gait therapy. This study investigated the effects of laterality and walking speed on gait symmetry during three conditions of walking on a treadmill, namely pushing while walking, walking with arm-swing, and walking while holding on a handlebar. All conditions were performed at three speeds: 1.5, 3, and 4 km/h. Symmetry was measured from bilateral gait cycle duration and cross-correlation function analysis performed in one gait cycle. Left-handers showed different characteristics from right-handers in various variables measured and not in mirror characteristics. Walking holding condition in general produced the greatest symmetry, confirmed the importance of this posture in gait therapy for improving symmetry among hemiparetic patients.
Optimizing the operating theatre environment

The operating theatre is a complex place. There are many potential factors which can interfere with surgery and predispose to errors. Optimizing the operating theatre environment can enhance surgeon performance, which can ultimately improve patient outcomes. These factors include the physical environment (such as noise and light), human factors (such as ergonomics), and surgeon‐related factors (such as fatigue and stress). As individual factors, they may not affect surgical outcome but in combination, they may exert a significant influence. The evidence for some of these working environment factors are examined individually. Optimizing the operating environment may have a potentially more significant impact on overall surgical outcome than improving individual surgical skill.
TITLE : TAM receptor tyrosine kinase function and the immunopathology of 1 liver disease 2 3 Short title : TAM receptor tyrosine kinases in liver disease

1 liver disease 2 3 Short title: TAM receptor tyrosine kinases in liver disease 4 5 AUTHORS: 6 S K Mukherjee1, A Wilhelm1, C G Antoniades1,2 7 Division of Digestive Diseases, Department of Medicine, Imperial College London 8 Institute of Liver Sciences, Division of Transplantation Immunology & Mucosal 9 Biology, King’s College London 10 Correspondence address: c.antoniades@imperial.ac. uk 11 Liver and antiviral unit, 10 floor QEQM building, South Wharf Road, St Mary’s 12 campus, Imperial College London 13 Telephone number: +44 (0) 20 3312 6454 14 Fax Number: +44 (0) 20 7724 9369 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Articles in PresS. Am J Physiol Gastrointest Liver Physiol (February 11, 2016). doi:10.1152/ajpgi.00382. 2015
Structures of genes nasA and nasB, encoding assimilatory nitrate and nitrite reductases in Klebsiella pneumoniae M5al

Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources during aerobic growth. Assimilatory nitrate and nitrite reductases convert nitrate through nitrite to ammonium. We report here the molecular cloning of the nasA and nasB genes, which encode assimilatory nitrate and nitrite reductase, respectively. These genes are tightly linked and probably form a nasBA operon. In vivo protein expression and DNA sequence analysis revealed that the nasA and nasB genes encode 92- and 104-kDa proteins, respectively. The NASA polypeptide is homologous to other prokaryotic molybdoenzymes, and the NASB polypeptide is homologous to eukaryotic and prokaryotic NADH-nitrite reductases. The narL gene product positively regulates expression of the structural genes for respiratory nitrate reductase, narGHJI. Surprisingly, we found that the nasBA operon is tightly linked to the narL-narGHJI region in K. pneumoniae, even though the nitrate assimilatory and respiratory enzymes serve different physiological functions.
Interlamellar cohesive strength in the vertical meridian of human eye bank corneas.

PURPOSE Previously, human corneal stromal interlamellar cohesive strength in the horizontal meridian was shown to be twice as strong peripherally as centrally (approximately 2.90 x 10(-1) versus 1.40 x 10(-1) N/mm). In the current study, stromal samples excised from the vertical meridian were studied to determine if meridional differences also exist. Precise knowledge about corneal stromal structure is warranted, because anisotropy can influence postoperative healing strength and corneal shape. METHODS Limbus-to-limbus stromal strips with a constant 2.2-mm width were obtained from the vertical meridians of 52 eyebank corneas (8 single, 22 pairs). Cohesive strength was recorded as samples were split at a constant 1.6 mm/s at 50% stromal depth. RESULTS The mean cohesive strength in the inferior periphery was found to be only two thirds the strength observed in the nasal or temporal periphery (1.96 x 10(-1) N/mm versus approximately 2.94 x 10(-1) N/mm @ 5 mm). The mean cohesive strength in the inferior periphery was also significantly less than the strength of the superior periphery (1.85 x 10(-1) N/mm +/- 8.83 x 10(-3) SEM versus 2.34 x 10(-1) +/- 1.37 x 10(-2) @ 4 mm from the central cornea; P = 0.0027). Unlike the samples from the horizontal meridian, which could be described by a profile symmetric about the central cornea, force profiles for the vertical data could be characterized with distinct, classifiable patterns that were generally asymmetric. Fellow corneas from a single donor tended to have strength profiles belonging to the same class (P = 0.035; chi-square), although some paired eyes exhibited profiles from distinctly different classes. CONCLUSIONS These data strongly support the concept of an anisotropic collagen macrostructure that is more complex than previously believed. This inherent structural anisotropy may become a significant determinant of corneal shape during ectatic disease and some forms of keratorefractive surgery.
Host enzyme induction of bacterial infection.

Early in the course of experimental pneumococcal infection in mice, a significant induction of the liver enzyme, tryptophan pyrrolase, occurs. The importance of endogenous adrenal secretions in producing enzyme induction was demonstrated. Changes in total liver protein synthesis during infection may parallel to a degree changes in tryptophan pyrrolase production and are also based on normal adrenal function. Alterations in these two apparently related phenomena indicate that a protein anabolic phase occurs during the course of experimental pneumococcal infection. The importance of these changes is discussed in terms of normal host defense mechanisms.
Allelic Expression Imbalance in the Human Retinal Transcriptome and Potential Impact on Inherited Retinal Diseases

Inherited retinal diseases (IRDs) are often associated with variable clinical expressivity (VE) and incomplete penetrance (IP). Underlying mechanisms may include environmental, epigenetic, and genetic factors. Cis-acting expression quantitative trait loci (cis-eQTLs) can be implicated in the regulation of genes by favoring or hampering the expression of one allele over the other. Thus, the presence of such loci elicits allelic expression imbalance (AEI) that can be traced by massive parallel sequencing techniques. In this study, we performed an AEI analysis on RNA-sequencing (RNA-seq) data, from 52 healthy retina donors, that identified 194 imbalanced single nucleotide polymorphisms(SNPs) in 67 IRD genes. Focusing on SNPs displaying AEI at a frequency higher than 10%, we found evidence of AEI in several IRD genes regularly associated with IP and VE (BEST1, RP1, PROM1, and PRPH2). Based on these SNPs commonly undergoing AEI, we performed pyrosequencing in an independent sample set of 17 healthy retina donors in order to confirm our findings. Indeed, we were able to validate CDHR1, BEST1, and PROM1 to be subjected to cis-acting regulation. With this work, we aim to shed light on differentially expressed alleles in the human retina transcriptome that, in the context of autosomal dominant IRD cases, could help to explain IP or VE.
Receiving Support and Care in Older Age: Comparing LAT Relationships With First Marriages, Remarriages, and Cohabitation

Living apart together (LAT) combines intimacy with autonomy and flexibility but, possibly, with lower commitment to exchanging support and care compared with first marriages, remarriages, and cohabitation of older adults. Data from 50- to 79-year-old respondents in the Family and Fertility Survey 2013 (Statistics Netherlands; N = 4,108) showed that older LAT partners are most often involved in exchanging emotional support. No differences were found in the receipt of daily care. Multivariate analyses showed that receiving support and care from the partner was associated with more health problems, higher quality of the partner relationship, and a broader support network. When in poor health, LAT partners were less likely to receive daily care, but not emotional support, from their partner compared with the other types. Partner relationships in later life are well equipped to provide emotional support, but partner care is facilitated largely by help from others.
Evaluation of Pharmacokinetic Interactions Between Vildagliptin and Digoxin in Healthy Volunteers

Vildagliptin is a novel antidiabetic agent that is an orally active, potent, and selective inhibitor of dipeptidyl peptidase IV, the enzyme responsible for degradation of the incretin hormones. This open‐label, randomized, 3‐period crossover study investigated the potential for pharmacokinetic interactions in 18 healthy subjects during coadministration of vildagliptin and digoxin. Subjects were randomized to receive each of 3 treatments: vildagliptin 100 mg qd, digoxin (0.5 mg, then 0.25 mg qd on days 2–7), and the combination vildagliptin/digoxin for 7 days. Coadministration of digoxin with vildagliptin had no effect on exposure to vildagliptin (geometric mean ratios [90% confidence interval]: AUC0‐24h, 0.99 [0.95–1.03]; Cmax, 0.95 [0.85–1.06]) or to digoxin (AUC0‐24h, 1.02 [0.94–1.12]; Cmax, 1.08 [0.97–1.20]). In addition, no changes in tmax, t1/2, and CL/F were observed for either drug. These results indicate that no dose adjustment is necessary when vildagliptin and digoxin are coadministered.
Fixed-bed photocatalysts for solar decontamination of water.

A solar decontamination process for water was developed using TiOt photocatalysts supported on silica-based material. The supported catalysts were systematically optimized with respect to catalyst type, catalyst dosage, silica-based support material, particle size, catalyst/ support bonding, and calcination temperature. The optimized supported catalysts outperformed an optimized slurry catalyst under identical operational conditions and had a reaction rate four times that of the slurry catalyst. Trichloroethylene (TCE) as a model compound was alsoused to investigate the impact of solar irradiance, influent concentration, pH value, and hydraulic loading
PTX3 plays a key role in the organization of the cumulus oophorus extracellular matrix and in in vivo fertilization

PTX3 is a prototypic long pentraxin that plays a non-redundant role in innate immunity against selected pathogens and in female fertility. Here, we report that the infertility of Ptx3–/– mice is associated with severe abnormalities of the cumulus oophorus and failure of in vivo, but not in vitro, oocyte fertilization. PTX3 is produced by mouse cumulus cells during cumulus expansion and localizes in the matrix. PTX3 is expressed in the human cumulus oophorus as well. Cumuli from Ptx3–/– mice synthesize normal amounts of hyaluronan (HA), but are unable to organize it in a stable matrix. Exogenous PTX3 restores a normal cumulus phenotype. Incorporation in the matrix of inter-α-trypsin inhibitor is normal in Ptx3–/– cumuli. PTX3 does not interact directly with HA, but it binds the cumulus matrix hyaladherin tumor necrosis factor α-induced protein 6 (TNFAIP6, also known as TSG6) and thereby may form multimolecular complexes that can cross-link HA chains. Thus, PTX3 is a structural constituent of the cumulus oophorus extracellular matrix essential for female fertility.
Application of a Theory of Enzyme Specificity to Protein Synthesis.

The suggestion of Lipmann' that the energy required to drive this reaction to the right came from adenosine triphosphate has been supported by the extensive work2 which has been discussed by the previous speakers on this symposium. While the detailed mechanism of this energy transfer has not been fully elucidated, the ATP apparently forms an amino acid anhydride. The fact that an anhydride of relatively high-energy content is the method of driving the reaction thermodynamically fits in with organic practice in which acyl anhydrides are the usual reagents for acylations. In addition to thermodynamic activation, there is a needed "kinetic activation. " For example, the acyl phosphates and acyl adenylates acylate amines non-enzymatically at appreciable rates in aqueous solution,3 but this reaction is certainly not rapid enough or selective enough to account for protein synthesis. The reaction of these acyl anhydrides to form peptides must be catalyzed, and it is this catalysis which is the subject of this paper. An analysis of why this linking of amino acids presents such formidable difficulties is revealing. The difficulty appears to be caused by a combination of requirements, each of which, taken singly, is rather easily satisfied. The first requirement is that an individual position in the protein be occupied by one and only one amino acid. This by itself is not a difficult condition to satisfy, since enzymatic reactions of equally high specificity are well known. The second requirement is that a highmolecular-weight polymer be produced. Again, this, by itself, is not a unique condition, since the enzymatic formation of high-molecular-weight molecules from a given monomer is also familiar, e.g., carbohydrate polymerization catalyzed by phosphorylases. Finally, the macromolecule is formed from many different monomer units, but this requirement is also easily achieved if the units are randomly arranged, e.g., polynucleotide formation by polynucleotide phosphorylase.4 However, the combined requirements, i.e., the synthesis of a macromolecule from many individual monomers to give a single specified sequence, is a problem of different magnitude. A mechanism involving an individual enzyme for each bond would be acceptable from the specificity point of view, but it would require an inconceivably large number of enzymes to form all the proteins of the cell. Moreover, there are other observed conditions, e.g., the necessity of feeding all the amino acids
Structure-activity relationships and in silico models of P-glycoprotein (ABCB1) inhibitors

Abstract 1. The efflux pump p-glycoprotein (P-gp/ABCB1) has received enormous attention in drug (xenobiotic) disposition due to its role in modulation of the drug availability and in protection of sensitive organs. 2. P-gp mediated efflux is one of main mechanisms for multidrug resistance in cancer cells. A main approach to reverse the resistance and restore the drug efficacy is to use specific inhibitors of P-gp that suppress the efflux activity. 3. This review summarizes the binding capabilities of known chemical inhibitors based on the analyses of structure–activity relationships, and computational modeling of the inhibitors as well as the binding site of P-gp protein. 4. The molecular models will facilitate the design of lead inhibitors as drug candidates. Also, it helps scientists in early drug discovery phase to synthesize chemical series with better understanding of their P-gp binding liabilities.