Miracle-dz/UniFAR-FTUs huggingface dataset:
New proteins from old diseases provide novel insights in cell biology

The lysosomal disease concept was developed by Hers in 1963. At the time, few could have imagined the breadth and depth of knowledge about cell biology that these disorders would reveal. With a collective hindsight of nearly four decades, it is fair to say that we have learned more about the lysosomal system of cells through the study of these rare diseases than by any other means. Given the advancements of the past year, it is apparent that some of the most significant insights are yet to come, as we delineate the last remaining and most enigmatic of these diseases.
Pulmonary artery intramural leiomyosarcoma mimicking pulmonary aneurysm

Chest radiography indicated a well-defined rounded mass at the left lung hilum in a 77-year-old former smoker. Chest computed tomography revealed a longitudinal saccular enlargement of the left pulmonary artery with surrounding soft tissue opacity. Resection of the left lower lobe with segment 1 + 2c was carried out to completely remove the dilated pulmonary artery. The resected specimen revealed obvious dilatation of the interlobar pulmonary artery and its branches. A small yellowish well-demarcated myxoid tumor was contained in this lesion, which was diagnosed as a primary pulmonary artery intramural leiomyosarcoma.
The Herpes Simplex Virus vhs Protein Induces Endoribonucleolytic Cleavage of Target RNAs in Cell Extracts

ABSTRACT The herpes simplex virus virion host shutoff (vhs) protein (UL41 gene product) is a component of the HSV virion tegument that triggers shutoff of host protein synthesis and accelerated mRNA degradation during the early stages of HSV infection. Previous studies have demonstrated that extracts from HSV-infected cells and partially purified HSV virions display vhs-dependent RNase activity and that vhs is sufficient to trigger accelerated RNA degradation when expressed as the only HSV protein in an in vitro translation system derived from rabbit reticulocytes. We have used the rabbit reticulocyte translation system to characterize the mode of vhs-induced RNA decay in more detail. We report here that vhs-dependent RNA decay proceeds through endoribonucleolytic cleavage, is not affected by the presence of a 5′ cap or a 3′ poly(A) tail in the RNA substrate, requires Mg2+, and occurs in the absence of ribosomes. Intriguingly, sites of preferential initial cleavage were clustered over the 5′ quadrant of one RNA substrate that was characterized in detail. The vhs homologue of pseudorabies virus also induced accelerated RNA decay in this in vitro system.
Transaminases in pertussis-parapertussis-bronchisepticus group of organisms.

In a previous paper(1) we reported on amino acid requirements of PertussisParapertussis-Bronchisepticus group of organisms, and in connection with this, it seemed to us necessary to have an information about transaminases of the same group of organisms. It has already been known that there is an enzyme or enzyme group concerning many kinds of transamination reaction in microorganisms. Feldman and Gunsalus (2) demonstrated in dried cells of Escherichia coli, Pseudomonas fluorescens, and Bacillus subtilis a series of transaminase producing glutamic acid from a-ketoglutamic acid with the following amino acids: aspartic acid, alanine, valine, leucine, norleucine, tryptophane, tyrosine, histidine, lysine, glycine and threonine. Rudman and Meister(3) also obtained cell-free extracts from E. coli, which catalize transamination reactions between glutamic acid and two groups of amino acids; namely, (a) phenylalanine, tyrosine, tryptophane, and aspartic acid, and (b) isoleucine, valine, leucine, norleucine, and norvaline. Millbank(4) further studied the transaminase system in the Alga chlorella. The present paper is concerned with the transaminase system of Pertussis-Parapertussis-Bronchisepticus group of organisms.
Highly linear SOI LDMOS power amplifier with combinated cascode structure

A fully on-chip SOI LDMOS linear PA for WLAN is implemented in a SOI LDMOS process. A cascode of SOI CMOS and SOI LDMOS is used to overcome the breakdown issue of the SOI CMOS transistor. An adaptive power cell (APC) and specially designed CG bias network are adopted to achieve linear performance. This proposed PA has gain of 24.3 dB and output power of 20.2 dBm for an 802.11n modulated signal with the EVM of -25dB.
5HT in Migraine Patients with Medication-Induced Headache

Whole blood 5HT levels were measured in seven female migraine sufferers with chronic daily headache due to medication abuse, before and after abrupt medication withdrawal. A statistically significant increase in 5HT levels, from mean 4.89 mmol/1 to mean 6.59 mmol/I (p < 0.05, Wilcoxon signed rank test), as well as a significant improvement in the number of headache-free days (p < 0.05, Wilcoxon signed rank test), occurred after 4 weeks of withdrawal. We conclude from this pilot study that 5HT may be important in the physiopathogenesis of chronic daily headache. Alternatively, reduced 5HT may be the result of chronic daily headache or else an epiphenomenon.
The relationship between aminoterminal propeptide of type III procollagen and heart rate variability parameters in heart failure patients: a potential serum marker to evaluate cardiac autonomic control and sudden cardiac death

Abstract Background: Cardiac extra-cellular matrix (ECM) fibrosis plays an important role in the pathophysiology of heart failure (HF). It may provide electrical heterogeneity and a substrate for arrhythmogenicity, which may cause sudden cardiac death (SCD). Methods: Twenty-one patients with manifestations of HF and a left ventricular ejection fraction (LVEF) ≤50% were enrolled. The median age was 62 years and median LVEF was 33%. Time- and frequency-domain analysis of heart rate variability (HRV) on 24 h ambulatory electrocardiography recording was assessed. Serum markers of ECM turnover including type I and III aminoterminal propeptide of procollagen (PINP and PIIINP), matrix metalloproteinase-2 and -9 (MMP-2 and MMP-9), and tissue inhibitor of metalloproteinase-1 (TIMP-1) were analyzed. Results: The serum PIIINP concentration was correlated significantly with standard deviation of all normal to normal R-R intervals (SDNN) (r=–0.722, p=<0.001), percentage of adjacent NN interval differences >50 ms (pNN50) (r=–0.528, p=0.014), percentage of adjacent NN interval differences >20 ms (pNN20) (r=–0.545, p=0.002), very low frequency (VLF) (r=–0.490, p=0.024), low frequency (LF) (r=–0.491, p=0.024), and high frequency (HF) (r=–0.513, p=0.018). PINP, MMP-2, -9, TIMP-1 were not correlated with time- and frequency-domain analysis of HRV. Conclusions: PIIINP was significantly correlated with time- and frequency-domain analysis of HRV in HF patients. PIIINP is a potential serological marker to evaluate cardiac autonomic control and risk of SCD in HF patients. Clin Chem Lab Med 2010;48:1821–7.
Na+/H+ exchange inhibition with HOE 642 improves recovery of the injured neonatal rabbit heart.

BACKGROUND The effects of inhibition of the Na+/H+ exchanger (NHE) on postischemic recovery of the injured neonatal rabbit heart were examined. The NHE may be an important mechanism for reperfusion injury in the neonate heart. The effects of two NHE inhibitors, HOE 642 (HOE) and 5-(N,N-dimethyl)-amiloride (DMA), given during hypothermic cardioplegic arrest, were evaluated. METHODS Isolated working crystalloid-perfused neonatal rabbit hearts were subjected to 10 min of normothermic ischemia to cause injury before undergoing 4 h of hypothermic (10 degrees C) cardioplegic arrest with a single dose of crystalloid solution (controls, n=21) or with the addition of 0.5 micromol/L HOE (n=24) or 30 micromol/L DMA (n=15). RESULTS Hearts subjected to HOE had improved recoveries of aortic flow when compared with controls at 15 min and 30 min of reperfusion (35.7+/-1.3 mL/min versus 26.2+/-1.4 mL/min, respectively, at 15 min, P<0.0001; 36.5+/-1.5 mL/min versus 23.6+/-1.6 mL/min, respectively, at 30 min, P<0.0001) and with DMA at 30 min (36.5+/-1.5 mL/min versus 29.9+/-1.9 mL/min, P=0.0214). Cardiac output and systolic pressure were also improved at 30 min in HOE hearts versus controls (P<0.0001). CONCLUSIONS NHE inhibition with HOE during cardioplegic arrest resulted in improved functional recovery of injured hearts. Further studies in blood-perfused neonatal preparations are warranted.
Profit-Based Unit Commitment with Emission Limitations: A Multiobjective Approach

This paper is on the problem of profit-based unit commitment with emission limitations. We propose a multiobjective approach to handle the problem with conflicting profit and emission objectives. A trade-off curve between profit and emission in a way to aid decision-makers concerning emission allowance trading is obtained due to this multiobjective approach. Numerical results based on the standard IEEE 30-bus test system illustrate the proficiency of the proposed approach.
Sodium caseinate induces increased survival in leukaemic mouse J774 model.

BACKGROUND Acute myeloid leukaemia is a neoplastic disease of haematopoietic stem cells. Although there have been recent advances regarding its treatment, mortality remains high. Consequently, therapeutic alternatives continue to be explored. In the present report, we present evidence that sodium caseinate (CasNa), a salt of the principal protein in milk, may possess important anti-leukaemic properties. MATERIALS AND METHODS J774 leukaemia macrophage-like cells were cultured with CasNa and proliferation, viability and differentiation were evaluated. These cells were also inoculated into BALB/c mice as a model of leukemia. RESULTS We demonstrated that CasNa inhibits the in vitro proliferation and reduces viability of J774 cells, and leads to increased survival in vivo in a leukaemic mouse model. CONCLUSION These data indicate that CasNa may be useful in leukaemia therapy.
Extreme sports: Extreme physiology. Exercise‐induced pulmonary oedema

We report five patients who presented to an on‐site medical team with concurrent haemoptysis and shortness of breath at a recent triathlon event. After initial management in the field, three of the five patients were transported to hospital via ambulance for further management, resulting in patients with haemoptysis and dyspnoea being 17 times more likely to require hospital transport. It is important to consider the differential diagnoses for this presentation, particularly exercise‐induced pulmonary oedema.
A Catalase-related Hemoprotein in Coral Is Specialized for Synthesis of Short-chain Aldehydes

Background: Biosynthetic transformation of fatty acid peroxides commonly is catalyzed by cytochromes P450. Results: A catalase-related hemoprotein in the coral Capnella imbricata converts 8R-hydroperoxy-eicosatetraenoic acid in a P450-type reaction to short-chain aldehydes. Conclusion: A catalase-related hydroperoxide lyase is identified in Animalia. Significance: The catalase-related hemoprotein has the catalytic competence of a P450. In corals a catalase-lipoxygenase fusion protein transforms arachidonic acid to the allene oxide 8R,9-epoxy-5,9,11,14-eicosatetraenoic acid from which arise cyclopentenones such as the prostanoid-related clavulones. Recently we cloned two catalase-lipoxygenase fusion protein genes (a and b) from the coral Capnella imbricata, form a being an allene oxide synthase and form b giving uncharacterized polar products (Lõhelaid, H., Teder, T., Tõldsepp, K., Ekins, M., and Samel, N. (2014) PloS ONE 9, e89215). Here, using HPLC-UV, LC-MS, and NMR methods, we identify a novel activity of fusion protein b, establishing its role in cleaving the lipoxygenase product 8R-hydroperoxy-eicosatetraenoic acid into the short-chain aldehydes (5Z)-8-oxo-octenoic acid and (3Z,6Z)-dodecadienal; these primary products readily isomerize in an aqueous medium to the corresponding 6E- and 2E,6Z derivatives. This type of enzymatic cleavage, splitting the carbon chain within the conjugated diene of the hydroperoxide substrate, is known only in plant cytochrome P450 hydroperoxide lyases. In mechanistic studies using 18O-labeled substrate and incubations in H218O, we established synthesis of the C8-oxo acid and C12 aldehyde with the retention of the hydroperoxy oxygens, consistent with synthesis of a short-lived hemiacetal intermediate that breaks down spontaneously into the two aldehydes. Taken together with our initial studies indicating differing gene regulation of the allene oxide synthase and the newly identified catalase-related hydroperoxide lyase and given the role of aldehydes in plant defense, this work uncovers a potential pathway in coral stress signaling and a novel enzymatic activity in the animal kingdom.
Use of Microalgae for Advanced Wastewater Treatment and Sustainable Bioenergy Generation

Abstract Given that sustainable energy production and advanced wastewater treatment for producing clean water are two major challenges faced by modern society, microalgae make a desirable treatment alternative by providing a renewable biomass feedstock for biofuel production, while treating wastewater as a growth medium. Microalgae have been known to be resilient to the toxic contaminants of highly concentrated organic wastewater (e.g., organic nitrogen, phosphorus, and salinity) and are excellent at sorbing heavy metals and emerging contaminants. Economic and environmental advantages associated with massive algae culturing in wastewater constitute a driving force to promote its utilization as a feedstock for biofuels. However, there are still many challenges to be resolved which have impeded the development of algal biofuel technology at a commercial scale. This review provides an overview of an integrated approach using microalgae for wastewater treatment, CO2 utilization, and biofuel production. The ma.
Teaching professionalism: general principles

There are educational principles that apply to the teaching of professionalism during undergraduate education and postgraduate training. It is axiomatic that there is a single cognitive base that applies with increasing moral force as students enter medical school, progress to residency or registrar training, and enter practice. While parts of this body of knowledge are easier to teach and learn at different stages of an individual's career, it remains a definable whole at all times and should be taught as such. While the principle that self-reflection on theoretical and real issues encountered in the life of a student, resident or practitioner is essential to the acquisition of experiential learning and the incorporation of the values and behaviors of the professional, the opportunities to provide situations where this can take place will change as an individual progresses through the system, as will the sophistication of the level of learning. Teaching the cognitive base of professionalism and providing opportunities for the internalization of its values and behaviors are the cornerstones of the organization of the teaching of professionalism at all levels. Situated learning theory appears to provide practical guidance as to how this may be implemented. While the application of this theory will vary with the type of curriculum, the institutional culture and the resources available, the principles outlined should remain constant.
Visualizing collapsible 3D data in a hybrid gis

In this paper we present a comprehensive set of advancements to our unique hybrid Geographical Information System (GIS). Although many existing commercial 3D GIS systems offer 2D views they are typically isolated from the 3D view in that they are presented in a separate window. Our system is a novel hybrid 2D/3D approach that seamlessly integrates 2D and 3D views of the same data. In our interface, multiple layers of information are continuously transformed between the 2D and 3D modes under the control of the user, directly over a base-terrain. In this way, our prototype GIS allows the user to view the 2D data in direct relation to the 3D view within the same window. In this work we progress the concept of a hybrid GIS by presenting a set of expanded capabilities within our distinctive system. These additional facilities include: landmark layers, 3D point layers, and chart layers, the grouping of multiple hybrid layers, layer painting, the merging of layer controls and consistent zooming functionality.
Temperature Sensing by an Olfactory Neuron in a Circuit Controlling Behavior of C. elegans

Temperature is an unavoidable environmental cue that affects the metabolism and behavior of any creature on Earth, yet how animals perceive temperature is poorly understood. The nematode Caenorhabditis elegans “memorizes” temperatures, and this stored information modifies its subsequent migration along a temperature gradient. We show that the olfactory neuron designated AWC senses temperature. Calcium imaging revealed that AWC responds to temperature changes and that response thresholds differ depending on the temperature to which the animal was previously exposed. In the mutant with impaired heterotrimeric guanine nucleotide–binding protein (G protein)–mediated signaling, AWC was hyperresponsive to temperature, whereas the AIY interneuron (which is postsynaptic to AWC) was hyporesponsive to temperature. Thus, temperature sensation exhibits a robust influence on a neural circuit controlling a memory-regulated behavior.
Cytogenetic risk determines outcomes after allogeneic transplantation in older patients with acute myeloid leukemia in their second complete remission: A Center for International Blood and Marrow Transplant Research cohort analysis

Allogeneic hematopoietic cell transplantation (HCT) offers curative potential to a number of older patients with acute myeloid leukemia (AML) in their first complete remission. However, there are limited data in the literature concerning post‐HCT outcomes for older patients in their second complete remission (CR2).
The long pentraxin PTX3 as a link among innate immunity, inflammation, and female fertility

The long pentraxin 3 (PTX3) is member of a complex superfamily of multifunctional proteins characterized by a cyclic multimeric structure. PTX3 is highly conserved in evolution and is produced by innate‐immunity cells in response to proinflammatory signals and Toll‐like receptor engagement. PTX3 plays complex, nonredundant functions in vivo, acting as a predecessor of antibodies, recognizing microbes, activating complement, facilitating pathogen recognition by phagocytes, and hence, playing a nonredundant role in resistance against selected pathogens. In addition, PTX3 is essential in female fertility by acting as a nodal point for the assembly of the cumulus oophorus hyaluronan‐rich extracellular matrix. Thus, the prototypic long pentraxin PTX3 is a multifunctional, soluble pattern recognition receptor acting as a nonredundant component of the humoral arm of innate immunity and involved in matrix deposition and female fertility.
On-line Multi-step Prediction of Short Term Traffic Flow Based on GRU Neural Network

Strengthened road traffic flow monitoring and forecasting can ease road traffic congestion and facilitate road traffic safety planning. Multi-step ahead of the ability to predict the traffic flow is particularly important. The monitoring data of road traffic flow is characterized by uncertainty and non-linearity. And using the existing methods to carry out multi-step prediction error will be very large. In this paper, based on these feature, we propose GRU neural network and autocorrelation analysis for multi-step prediction. We make this model dynamically update the network with the input of the measured real-time data, namely on-line prediction, to work effectively and constantly. Through the theoretical derivation and simulation analysis, it is shown that the prediction accuracy of the proposed GRU prediction model is improved. The model can be used as an effective method for multi-step traffic prediction.
th Workshop “ Automotive HMI ” : Vehicles in the Transition from Manual to Automated Driving

Automotive user interfaces and, in particular, automated vehicle technology pose a plenty of challenges to researchers, vehicle manufacturers, and third-party suppliers to support all diverse facets of user needs. To give an example, they emerge from the variation of different user groups ranging from inexperienced, thrill-seeking young novice drivers to elderly drivers with all their natural limitations. To allow assessing the quality of automotive user interfaces and automated driving technology already during development and within virtual test processes, the proposed workshop is dedicated to the quest of finding objective, quantifiable quality criteria for describing future driving experiences. The workshop is intended for HCI, AutomotiveUI, and “Human Factors” researchers and practitioners as well for designers and developers. In adherence to the conference main topic “Spielend einfach interagieren”, this workshop calls in particular for contributions in the in the area of human factors and ergonomics (user acceptance, trust, user experience, driving fun, natural user interfaces, etc.) and artificial intelligence (predictive HMIs, adaptive systems, intuitive interaction).
Targeted O 2 delivery by low-P 50 hemoglobin : a new basis for O 2 therapeutics

Tsai, Amy G. , Kim D. Vandegriff, Marcos Intaglietta, and Robert M. Winslow. Targeted O2 delivery by low-P50 hemoglobin: a new basis for O2 therapeutics. Am J Physiol Heart Circ Physiol 285: H1411–H1419, 2003. First published June 12, 2003; 10.1152/ajpheart.00307. 2003. —To assess O2 delivery to tissue by a new surface-modified, polyethylene glycol-conjugated human hemoglobin [MP4; PO2 at 50% saturation of hemoglobin (P50); 5.4 mmHg], we studied microcirculatory hemodynamics and O2 release in golden Syrian hamsters hemodiluted with MP4 or polymerized bovine hemoglobin (PolyBvHb; P50 54.2 mmHg). Comparisons were made with the animals’ hemodiluted blood with a non-O2 carrying plasma expander with similar solution properties (Dextran-70). Systemic hemodynamics (arterial blood pressure and heart rate) and acid-base parameters were not correlated with microhemodynamics (arteriolar and venular diameter, red blood cell velocity, and flow). Microscopic measurements of PO2 and the O2 equilibrium curves permitted analysis of O2 release in precapillary and capillary vessels by red blood cells and plasma hemoglobin separately. No significant differences between the groups of animals with respect to arteriolar diameter, flow, or flow velocity were observed, but the functional capillary density was significantly higher in the MP4-treated animals (67%) compared with PolyBvHbtreated animals (37%; P 0.05) or dextran-treated animals (53%). In the PolyBvHb-treated animals, predominant O2 release (both red blood cells and plasma hemoglobin) occurred in precapillary vessels, whereas in MP4 animals most of the O2 was released from both red blood cells and plasma hemoglobin in capillaries. Base excess correlated directly with capillary O2 release but not systemic O2 content or total O2 release. Higher O2 extraction of both red blood cell and plasma hemoglobin in capillaries represents a new mechanism of action of cell-free hemoglobin. High O2 affinity appears to be an important property for cell-free hemoglobin solutions.