Miracle-dz/UniFAR-FTUs huggingface dataset:
The Involvement of ERCC2/XPD and ERCC6/CSB Wild Type Alleles in Protection Against Aging and Cancer.

BACKGROUND DNA helicases maintain genome stability, and their deficiency is associated with disorders resembling premature aging as well as contributes to carcinogenesis. Their functions are determined by the respective genes encoding nucleotide excision repair initiating proteins, e.g. XPD and CSB. OBJECTIVE The present study aimed to investigate the influence of genetic variations in ERCC2/XPD (rs1799793, rs13181) and ERCC6/CSB (rs2228526, rs2228528) loci on lifespan and developing age-related bladder cancer focusing on homozygous wild type alleles. METHOD The allelic variants were identified in 354 clinically healthy controls and 418 bladder cancer patients using the PCR-RFLP method. RESULTS The age-depended increase in frequencies of homozygous carriers of wild-type XPD 312Asp and XPD 751Lys alleles was observed among controls, especially among subjects over 80 years (r = 0.67, p = 0.012). The statistically significant correlation was also found between the frequency of homozygous wild type alleles at all tested loci and age in healthy population over 60 years (r = 0.35, p = 0.046) suggesting the relationship between lifespan and longevity, on one hand, and normal functioning of these genes and their products, on the other hand. Homozygous carriers of wild type alleles were less susceptible to bladder cancer, tumor invasion, increase in grade of malignancy and recurrence, but their effects were specific with respect to clinicopathological and lifestyle characteristics. CONCLUSION Homozygous wild type alleles encoding XPD and CSB proteins with optimal properties were shown to affect human lifespan, risk of developing bladder cancer, its progression and recurrence under certain conditions.
Reconstitution of protein kinase C‐induced contractile Ca2+ sensitization in Triton X‐100‐demembranated rabbit arterial smooth muscle

1 Triton X‐100‐demembranated smooth muscle loses Ca2+‐sensitizing responsiveness to protein kinase C (PKC) activators while intact and α‐toxin‐permeabilized smooth muscles remain responsive. We attempted to reconstitute the contractile Ca2+ sensitization by PKC in the demembranated preparations. 2 Western blot analyses showed that the content of the PKC α‐isoform (PKCα) was markedly reduced and that the smooth muscle‐specific protein phosphatase‐1 inhibitor protein CPI‐17 was not detectable, while the amount of calponin and actin still remained similar to those of intact strips. 3 Unphosphorylated recombinant CPI‐17 alone induced a small but significant contraction at constant Ca2+. Isoform‐selective PKC inhibitors inhibited unphosphorylated but not pre‐thiophosphorylated CPI‐17‐induced contraction, suggesting that in situ conventional PKC isoform(s) can phosphorylate CPI‐17. 4 Exogenously replenishing PKCα alone did not induce potentiation of contraction and only slowly increased myosin light chain (MLC) phosphorylation at submaximal Ca2+. 5 PKC in the presence of CPI‐17, but not the [T38A]‐CPI mutant, markedly induced potentiation of both contraction and MLC phosphorylation. CPI‐17 itself was phosphorylated. 6 In in vitro experiments, CPI‐17 was a much better substrate for PKCα than calponin, caldesmon, MLC and myosin. 7 Our results indicate that PKC requires CPI‐17 phosphorylation at Thr‐38 but not calponin for reconstitution of the contractile Ca2+ sensitization in the demembranated arterial smooth muscle.
Computer simulation of liquid/liquid interfaces. II. Surface tension-area dependence of a bilayer and monolayer

A constant normal pressure‐surface tension algorithm for molecular dynamics simulation, developed in the preceding paper, was used to laterally expand and compress the surface area of a dipalmitoylphosphatidylcholine (DPPC) lipid bilayer. Then, from simulations carried out at constant normal pressure and surface area, values of the surface tension and other thermodynamic variables such as the internal energy and system volume were determined at four different values of the surface area per lipid, 60.0, 65.1, 68.1, and 72.1 A2. The surface tension shows dramatic variations with area, going from 6 to 60 dyn/cm at areas per molecule of 65.1 and 68.1 A2, respectively. An approximate thermodynamic analysis indicates that an area of 68.1 A2/lipid is the closest of the four to the free energy minimum for this system, in agreement with experimental measurements. The effect of surface area changes on the calculated deuterium order parameters, which can be compared with those obtained from nuclear magnetic resonanc.
Transport Properties of Pure Spin Currents in a Polycrystalline Gd Wire

We have investigated the magneto- and spin-current-transport properties in patterned Gd wires in order to understand the interplay between the spin current and the spin entropy. From the magneto-transport measurements, the electrical resistance was found to decrease with decreasing the spin entropy because of the suppression of the spin fluctuation. We also found that the Gd acts as a strong absorber both for the ac and dc pure spin currents from the ferromagnetic resonance and nonlocal spin injection experiments.
The Chronically Mentally Ill in Community Facilities

The quality of life of chronically mentally ill patients in acute wards in a district general hospital, a hostel ward and group homes was compared. Within the spectrum of care of these patients, the severity of psychopathology corresponded to their placement. Analysis, including adjustments for the influence of psychopathology, showed differences between the three types of facility. Although differences existed between all types of care, residents in group homes and the hostel ward shared more similarities in quality of life than those in the district general hospital. Problems of caring for the chronically mentally ill on acute wards are highlighted.
The Effect of Experimental Stress upon the Photically Activated EEG.

1. MAPSON,L. W. , and D. R. Nature, 167,'975 GODDARD, (1951). 2. CONN, E. E. and VENNESLAND, B. Ibid. , 976. 3. -. J. Biol. Chem., 192,1 7 (1951). 4. B~ELDRUII . X. U. and TARR. H. L. A. Biochewc. J. 29. 108 (1995). 5. TUNNICLIFFE, H. E. Ib id . , 19,199 (1925). 6. ABDERHALDEN. and E. PRuqers Arch. . E. WERTHEIMER. ges. Phusiol. , 199,336 (1923F. 7. BRCNDLER. H Science. 114.61 (1951). 8. HOGEBOO~~I, G . T. J. Biol. Chem., 176, G. H. , and BARRY, 935 (1948). 9. SHINOKARA, K. Ib id . , 109,665 (1935). 10. SWART, E. A. , ROMANO, A. H. , and WAICSIlAN, S. A. PrOC. Soc. Eopt l . Biol. Xed. , 73, 376 (1950).
Activity Measurements of Planktonic Microbial and Microfouling Communities in a Eutrophic Estuary

[3H]thymidine incorporation, the rate of reduction of iodonitrotetrazolium violet (INT) to INT formazan normalized to DNA, and the ratio of ATP to DNA were adapted to measure the activity of attached and unattached microbial assemblages of Bayboro Harbor, Fla. Activity measurements by [3H]thymidine incorporation were made of cells attached to polystyrene culture dishes, in unfiltered water samples, and in the <1-μm-filtered fraction. In most cases, the activity of attached cells was greater than that of unattached cells either in unfiltered water samples or in the <1-μm fraction. The calculated thymidine incorporation rates for cells in the >1-μm fraction were higher than those for cells either in unfiltered water or in the <1-μm-filtered fraction. By the rate of reduction of INT to INT formazan normalized to DNA and by ATP-to-DNA ratios, attached cells were also more active than cells in unfiltered water samples. These results indicate that the microenvironment afforded by attachment is a more beneficial habitat for microbial growth. Reasons for greater activity by natural populations of attached bacteria are discussed.
Neuropathologic correlates of white matter hyperintensities

Objective: White matter hyperintensities (WMH) are commonly seen on neuroimaging scans, but their underlying histopathologic substrate is unclear. The aim of this work was to establish the pathologic correlates of WMH in unselected elderly cases using two study designs. To avoid potential bias from comparisons of different anatomic regions, study 1 compared, region-by-region, the severity of WMH determined in vivo with measures of each of the major white matter (WM) components. Study 2 compared the histopathology of WMH with normal WM. Methods: Study 1: The periventricular and deep WM regions of three lobes in 23 brains with in vivo MRI scans were investigated using histologic and immunohistochemical stains. The severity of each pathologic measure was correlated with WMH severity determined using the Scheltens scale. Study 2: Lesioned and nonlesioned areas identified by postmortem MRI in the frontal WM of 20 brains were examined histologically and immunohistochemically. Results: No single pathologic variable correlated with the severity of WMH; however, a multiple stepwise regression analysis revealed that vascular integrity predicted total Scheltens score (β = −0.53, p = 0.01). Comparison of lesioned and nonlesioned areas demonstrated that vascular integrity was reduced in WMH [t(18) = 3.79, p = 0.001]. Blood–brain barrier integrity was also found to be reduced in WMH [t(5) = −5.31, p = 0.003]. Conclusions: White matter hyperintensities (WMH) involve a loss of vascular integrity, confirming the vascular origin of these lesions. This damage to the vasculature may in turn impair blood–brain barrier integrity and be one mechanism by which WMH evolve.
ENDONUCLEOLYTIC INCISION OF UVB‐IRRADIATED DNA

Abstract. Ultraviolet irradiation of DNA produces a variety of pyrimidine damage. In addition to the well‐known photoproducts, pyrimidine dimers and 6‐4′(pyrimidin‐2'one)pyrimidines, DNA sequence analysis has shown that E. coli endonuclease III incises broad‐spectrum UV‐irradiated DNA at cytosines. The same enzyme incises DNA oxidized by osmium tetroxide at damaged thymines. The wavelength dependence of endonuclease III incision of irradiated DNA was examined. DNA substrates were irradiated by monochromatic light ranging from 250 to 320 nm. Maximal enzymic cleavage was obtained with substrates irradiated by wavelengths between 265 and 285 nm, with peak activity against DNA irradiated at 280 nm. Therefore, these photoproducts are optimally formed at biologically significant wavelengths and may be involved in actinic carcinogenesis.
Generation quiz with genetic algorithm based on bloom's taxonomy classification in serious game based virtual environments

Learning with seorius game based virtual environments provide immersive and motivation. One aspect of learning is evaluation. But evaluation provides anxiety on the learner, as question that is too difficult. In this paper will describe how to generate questions in evaluation(quiz) based on bloom taxonomy with composition 25:50:25 for the types of questions C1, C2, and C3. The question generation used genetic algorithms and implemented in the virtual learning environment (VLE) through SLOODLE for 5th grades math course. System testing used compare genetic algorithm (GA) with random method. The result is the question composition of the generation of GA better than the generation of random method.
Crystal structure of trehalose‐6‐phosphate phosphatase–related protein: Biochemical and biological implications

We report here the crystal structure of a trehalose‐6‐phosphate phosphatase–related protein (T6PP) from Thermoplasma acidophilum, TA1209, determined by the dual‐wavelength anomalous diffraction (DAD) method. T6PP is a member of the haloacid dehalogenase (HAD) superfamily with significant sequence homology with trehalose‐6‐phosphate phosphatase, phosphoserine phosphatase, P‐type ATPases and other members of the family. T6PP possesses a core domain of known α/β‐hydrolase fold, characteristic of the HAD family, and a cap domain, with a tertiary fold consisting of a four‐stranded β‐sheet with two α‐helices on one side of the sheet. An active‐site magnesium ion and a glycerol molecule bound at the interface between the two domains provide insight into the mode of substrate binding by T6PP. A trehalose‐6‐phosphate molecule modeled into a cage formed by the two domains makes favorable interactions with the protein molecule. We have confirmed that T6PP is a trehalose phosphatase from amino acid sequence, three‐dimensional structure, and biochemical assays.
Characteristics of adult dog owners in New Zealand

Abstract AIM: To investigate the characteristics of adult dog owners in New Zealand, and to identify factors that influence dog ownership. METHODS: A 12-page questionnaire comprising 48 questions was sent to 2,000 people throughout New Zealand randomly selected from the electoral roll. RESULTS: Seven hundred and twenty-seven completed questionnaires were received from 1,750 questionnaires successfully delivered, a response rate of 42%. Twenty-nine percent (211/727) of respondents currently owned dogs, and 32% (233/727) had never owned a dog; the remaining 39% (283/727) had some history of dog ownership. The level of dog ownership was influenced by the type of dwelling, region, age, marital status, presence and age of children, and type of personality. CONCLUSIONS: Dog owners in New Zealand are likely to be aged between 18 and 55 years, educated to secondary school level or above, live in a rural or suburban area, be married or in a de-facto relationship with children over the age of one year, and consider themselves to be of positive character.
Advanced Driver Assistance Systems

Description: Advanced driver assistance systems (ADAS) are systems developed to automate/adapt/enhance vehicle systems for safety and better driving. Safety features are designed to avoid collisions and accidents by offering technologies that alert the driver to potential problems, or to avoid collisions by implementing safeguards and taking over control of the vehicle. Adaptive features may automate lighting, provide adaptive cruise control, automate braking, incorporate GPS/ traffic warnings, connect to smartphones, alert driver to other cars or dangers, keep the driver in the correct lane, or show what is in blind spots. Demand for advanced driver-assistance systems, especially those that help with monitoring, warning, braking, and steering task is expected to increase over the next decade, fueled largely by regulatory and consumer interest in safety applications that protect drivers and reduce accidents.
Origin of cerebellar projections to the region of the oculomotor complex, medial pontine reticular formation, and superior colliculus in new world monkeys: A retrograde horseradish peroxidase study

Cerebellar projections to oculomotor‐related brainstem regions were studied in four groups of New World (capuchin, squirrel) monkeys by using the retrograde transport of horseradish peroxidase (HRP) to determine the origin of the principal cerebellar influence on eye movement.
Ectopic testes in the Norway rat.

Animals with ectopic testes, some unilateral (on either the right or left side) and some bilateral, have appeared in a colony of King-Holtzman rats. This heritable defect has been given the genetic symbol "ect. " Females carrying the gene are unaffected by it. When such females are bred to apparently normal males, they produce no ect offspring. Since 25% of their F2 generation carry the gene, it is assumed it is autosomal recessive. Studies involving selective breeding for males with ectopic testes indicate that some males have genomes resistant to the expression of the ect gene and that the apparent thresholds essential to left side, right side, and bilateral expression appear to be a secondary control of the multifactorial type.
Actualidades de fármacos vasopresores e inotrópicos en anestesia

En los ultimos 15 anos diversos farmacos vasopresores e inotropicos han sido introducidos a la practica clinica, incrementandose el arsenal terapeutico para aquellos enfermos que presentan disfuncion cardiovascular aguda o descompensada. El anestesiologo con mayor frecuencia se enfrenta a escenarios clinicos donde se presenta inestabilidad hemodinamica aguda y debe estar familiarizado con los nuevos agentes terapeuticos que permiten un manejo integral en el preoperatorio, el transoperatorio y el postoperatorio. El objetivo de esta revision es dar a conocer las actualidades en el manejo de los farmacos vasopresores e inotropicos en el contexto del enfermo que presenta falla cardiovascular y requiere de la administracion de estos farmacos y que sera sometido a un procedimiento anestesico-quirurgico.
Growth and survival of reovirus in intestinal tissue: role of the L2 and S1 genes

Reovirus serotype 1 Lang can be recovered in high titer from the intestines of neonatal mice up to day 8 after peroral inoculation. By contrast, reovirus serotype 3 Dearing cannot be recovered from intestinal tissue past day 4 after peroral inoculation. This difference between the two reoviruses was mapped by using reassortants generated from nonmutagenized laboratory stocks. When the L2 and S1 genes of reovirus serotype 3 Dearing were present in reassortants, the reassortants behaved like serotype 3 Dearing in exhibiting a decreased capacity to be recovered from intestinal tissue. Likewise, viruses which contained the L2 and S2 genes from serotype 1 Lang exhibited an enhanced capacity to grow and survive, which is characteristic of serotype 1 Lang. Thus, the capacity of reovirus to survive in intestinal tissue was determined by the L2 and S1 genes.
ORGANIZATIONAL CHANGE WITH THE SYSTEM AND COMPLEXITY THEORIES IN MIND

The ability to successfully manage both internal and external generated changes has become a major source of competitive advantage for business organizations. This study tries to explore the system and complexity theory in line with organization change. The paper reviews organizational change through system theory, various critiques of system theory, complexity theory, dynamics of complex system, characteristics of complex system, and the implication of system and complexity theory for organization using relevant literatures. A good understanding of the two paradigms would become useful in addressing complex organizational dilemmas that may result as the organization tries achieving its goal and objective in a dynamic internal and external environment.
Employee organizational commitment and hospital performance

Background: There is widespread evidence of the purported benefits of employee organizational commitment (EOC) and its impact on both individual and organizational performance. This study contributes to this literature by providing a unique insight into this relationship, focusing on the interrelationship between EOC with hospital performance and the role of the provision of adequate facilities in eliciting EOC. Purpose: The aim of this study was to introduce and empirically examine a new theoretical model in which it is argued that the performance of hospitals with regard to the provision of adequate facilities (medical facilities, support facilities, and staff resources) influences the level of EOC, which in turn influences hospital performance with regard to patient care and operational effectiveness. Methodology/Approach: To examine the interrelationships between the provision of adequate facilities, EOC, and hospital performance, the study utilizes a survey of hospital managers. Results: The findings support the theoretical model, with the provision of support facilities and staff resources positively indirectly associated with both patient care and operational effectiveness through their impact on EOC. Conclusion: The findings highlight the importance of providing adequate facilities and EOC within hospitals and suggest that CEOs and general managers should try to enhance the provision of such resources in an attempt to elicit EOC within their hospitals. Practice Implications: The findings suggest that managers should try to enhance their provision of adequate facilities in order to elicit EOC and enhance hospital performance. With regard to medical facilities, they should consider and incorporate the latest technology and up-to-date equipment. They should also provide adequate staff resources, including appropriate numbers of beds, nurses, and doctors, to prevent “fatigue” (West, 2001, p. 41) and provide adequate support facilities.
Therapy and prophylaxis of brain metastases

Metastases of various tumors to the brain account for the majority of brain cancers, and are associated with a poor prognosis. The most common primary sites are lung, breast, skin, kidney and colon; 10–40% of cancer patients develop brain metastases during the course of the disease. The incidence of brain metastasis appears to be rising; reasons may include better therapies for the systemic disease with longer survival of cancer patients but lower efficiency against brain metastases. In this article, we will discuss the conventional treatment with surgery, radiosurgery, radiotherapy and chemotherapy, but also new directions in the management of solid brain metastases. While general therapeutic nihilism should be avoided, it is important to recognize that the number of brain metastases, the extent of the systemic disease and also the tumor type have to be taken into account when choosing individual treatment regimens. Finally, special emphasis will be put on established and future approaches to prevent the disease. We thus aim to provide a framework for treating patients with different presentations of brain metastases, and to highlight important avenues for research.
A Short-Term Intervention to Enhance Cognitive and Affective Functioning in Older Adults

Objective: This study was designed to investigate the benefits of a short-term intervention for older adults that targeted cognitive functioning and quality of life issues important for independent living. Method: One hundred twenty-four community-dwelling participants (aged 60 to 86) took part in one of three study conditions: theater arts (primary intervention), visual arts (non-content-specific comparison group), and no-treatment controls. Results: After 4 weeks of instruction, those given theater training made significantly greater gains than did no-treatment controls on both cognitive and psychological well-being measures. A comparison of theater and visual arts training showed fewer benefits in fewer areas for visual arts. Discussion: The authors suggest reasons why various aspects of theater training appear to enhance healthy aging.
Simulating dust accumulation

Dust accumulation is a common natural phenomenon. Modeling of dust accumulation, however, has not been well studied. We present a technique to model the amount of dust that settles on object surfaces based on the properties of surfaces and the geometry of objects. Since many researchers have studied the scattering of light by air-borne dust, we do not consider it here. We illustrate our techniques with images of dusty objects rendered using our dust texture module within Rayshade 4.0. 6, a public domain ray tracer. >