Miracle-dz/UniFAR-FTUs huggingface dataset:
Doxorubicin-loaded Pluronic P123-PEG2000-DSPE micelles reverse the drug resistance of breast cancer cells

A new mixed nano-micelle, doxorubicine (Dox)-loaded Pluronic P123/poly (ethylene glycol) 2000-distearoylphosphatidylethanolamine nanomicelles mixed micelles (P123-PEG2000-DSPE (Dox)) were developed to investigate the reversal effects of nanoformulations on breast carcinoma (BC) multidrug resistance (MDR). This study aimed to explore the reversal effects of nanoformulations on BC multidrug resistance. The P123-PEG2000-DSPE (Dox) mixed micelles were prepared and then characterized by the physicochemical parameters with the dynamic light scattering method, drug release profile, and antineoplastic activity including dynamic light scattering method, MTT, immunofluorescence, Western blot and Annexin V-PI in BC MCR-7 cells and MCF-7R, a BC drug resistant cell line. P123-PEG2000-DSPE (Dox) reversed drug resistance with a better effect than PEG2000DSPE (Dox) in the cells via suppressing the expression of MDR1 and p-gp, decreasing drug efflux, and increasing cellular endocytosis. Furthermore, for the Dox-loaded groups, the cytotoxicity of P123-PEG2000-DSPE (Dox) was better than that of PEG2000-DSPE (Dox) and Dox. PEG2000-DSPE and P123-PEG2000-DSPE, the empty drug carriers, had no cytotoxicity. These findings indicated that P123-PEG2000-DSPE (Dox) micelles could effectively reverse drug resistance in BC cells, which is a promising antitumor drug delivery system.
Vehicle to Grid Technology ( V 2 G ) For the Application of Electric Traction

A grid connected PV system with batteries as the energy storage element for application to electric train has been proposed in this paper. Battery packs and PV arrays are cascaded together to the utility through a bidirectional dc converter and boost converter. The PV module terminal voltage is regulated by a switched dc-dc step up boost converter, which uses the Incremental Conductance algorithm to track the maximum power point (MPP). A bidirectional DC/DC converter efficiently regulates flow of power between battery and grid. Bidirectional Inverter provides supporting grid functions which enhances grid stability. Simulation of the proposed system is performed in MATLAB/SIMULINK and the results are found to be satisfactory.
Assessing clinical practices and beliefs among providers following women diagnosed or at-risk for Fabry disease

For women with Fabry disease, there are several factors which can influence the level of a patient’s engagement with the healthcare system to manage her disease. Unfortunately, some of these factors have the potential to reduce the likelihood that a female patient will become an active participant in her own medical management because of the influence on the patient’s perception of her own disease susceptibility and personal health beliefs, thus increasing her risk for negative Fabry-disease related health outcomes. These factors, such as the past history of labeling women as asymptomatic carriers and the influence of disease burden on the mental health of the patient, are structural and psychosocial in nature, and collectively contribute to the barriers perceived by women to engaging in disease management. Recognizing the multi-faceted origins and complexity of these factors, our team developed a three part project to examine contributing factors and their origins to these barriers. Parts one and two of this project focused on characterizing the health beliefs of females with Fabry disease using the Health Belief Model and identified patients’ perceived severity, susceptibility, and benefits to engaging in Fabry disease related care. This study, part three of the project, focuses on a different source, aiming to characterize Fabry healthcare providers’ clinical practices and beliefs in order to assess their potential role in the contributions to these barriers. By surveying 58 healthcare providers from a variety of locations, potential areas of clinical practice that may influence the health beliefs and barriers of women with Fabry disease were identified, including discrepancies in clinical practice across providers of differing levels of experience, the inconsistent adherence to clinical practice guidelines, and use of the term “carrier” by providers to describe female patients. Understanding the presence of these factors allows for strategies to be developed that can address these barriers, and in turn, optimize clinical health outcomes for female Fabry patients. Furthermore, these strategies can be applied to address barriers for women affected by other X linked conditions, thus signifying the potential for a public health intervention based on the results of this three part project.
thromboem-Follow-up after acute Pulmonary Embolism Predicting chronic thromboembolic pulmonary hypertension and post-pulmonary embolism syndrome

In addition to among others major bleeding from anticoagulant therapy and recurrent venous thromboembolism (VTE), patients who survived acute pulmonary embolism (PE) face an increased risk of chronic functional limitations and decreased quality of life. In recent years, this latter complications have been better framed within the evolving definition of “post-PE syndrome” of which chronic thromboembolic pulmonary hypertension (CTEPH) represents the most extreme presentation. The post-PE syndrome in all its aspects is a frequent and clinically relevant long-term complication of PE but -except for CTEPHhas been largely understudied. There is great need to better define and understand the natural course of acute PE, to predict the development of the post-PE syndrome and to evaluate the potential benefits evolving treatments such as cardiopulmonary rehabilitation. Schlüsselwörter Lungenembolie, CTEPH, chronisches Lungenembolie-Syndrom, Lebensqualität
miR-204 regulates HMGA2 to suppress the proliferation and promote the apoptosis of esophageal squamous carcinoma cells

Objective: To investigate the role of miR-204 in esophageal squamous cell carcinoma (ESCC) and to determine the potential molecular mechanisms underlying its action. Methods: miR-204 expression in ESCC tissues and cell lines was detected through qRT-PCR. The targeted regulation of miR-204 on the target gene HMGA2 was verified through dual-luciferase reporter genes. Meanwhile, the effect of the miR-204/HMGA2 axis on ESCC cells was investigated through cell CCK-8 assay and flow cytometry. Results: miR-204 expression in ESCC tissues was markedly lower than that in para-carcinoma tissues and low miR-204 expression was closely correlated with the lymph node metastasis and tumor stage of ESCC patients. Moreover, miR-204 expression in human ESCC cell lines TE-13, Eca-109, KYSE-410 and EC9706 was notably lower than that in normal human esophageal cell line Het-1A. miR-204 could bind with the binding site at the 3’ UTR of HMGA2, thus markedly down-regulating the luciferase activity. Up-regulating miR-204 expression in KYSE-410 cells could remarkably down-regulate the HMGA2 mRNA and protein expression, suppress the cell proliferation activity, and promote cell apoptosis. In addition, Up-regulating HMGA2 expression could reverse these effects. Conclusion: miR-204 expression is abnormally down-regulated in ESCC, and miR-204 overexpression can suppress the proliferation and promote the apoptosis of ESCC cells, the mechanism of which may be related to the targeted regulation on HMGA2.
Monotropein accelerates chondrocyte progression in osteoarthritis by alleviating TNF-α induced inflammation through regulation of MAPK/NF-κB signaling pathway

Background: Osteoarthritis (OA) is a common degenerative musculoskeletal disease in elderly people over 50 years old. Inflammatory response of chondrocytes in OA during treatment vitiated the treatment outcomes. Antiinflammatory ingredient monotropein has been used in the treatment of OA. However, the molecular mechanism of monotropein in inflammatory response of chondrocytes is unclear. Methods: Cell viability of primary chondrocytes and ATDC5 cells was determined by CCK8 assay. Cell apoptosis was examined by flow cytometry assay. The production of nitric oxide (NO) was detected using Griess reagent. The release of inflammatory cytokines interleukin-1 (IL-1), interleukin-6 (IL-6) and interleukin-12 (IL-12) was measured by enzyme linked immunosorbent assay (ELISA). The expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was assessed by qRT-PCR. Protein expression of iNOS, COX-2, matrix metalloproteinases (MMP-1, MMP-3, MMP-13), p-p65, p65, IkapaB kinase proteins (p-IKK-α/β, IKK-α, IKK-β), inducible nuclear IκB proteins (p-IκB-α, IκB-α), p-ERK1/2, ERK1/2, p-p38 and p38 was analysed by western blot assay. Results: Monotropein exhibited negligible influences on chondrocyte survival. In addition, monotropein alleviated tumor necrosis factor-α (TNF-α) induced inhibition on viability and promotion on apoptosis in chondrocytes. The production of NO, inflammatory cytokines IL-1, IL-6 and IL-12 was enhanced by TNF-α and suppressed gradually by monotropein. Moreover, monotropein attenuated TNF-α induced release of iNOS, COX-2 MMP-1, MMP-3 and MMP-13 in chondrocytes. Besides, monotropein blocked TNF-α induced mitogen-activated protein kinase (MAPK)/NF-κB activation in chondrocytes. Conclusion: Monotropein accelerates chondrocyte progression of in OA by alleviating TNF-α induced inflammation through regulation of MAPK/NF-κB signaling pathway, providing novel therapy strategy for OA.
Patient Blood Management: An Internist's Perspective

Patient blood management (PBM) is a patient-specific, evidencebased and systematic approach to optimize the management of patient and blood transfusion for quality and effective patient care. It aims at improving patient outcomes through the rational and safe use of blood products and minimizing unnecessary exposure to it [1]. The concept of PBM is not only “blood conservation” but also “blood management” which includes the preventive approaches to maintain and optimize hemoglobin (Hb) level and hemostasis [2]. The goal of PBM is not merely to avoid or withhold transfusions, but to timely apply evidence-based medical and surgical concepts designed to manage anemia, optimize hemostasis, and minimize blood loss to improve patient outcomes by relying on a patient’s own blood rather than on donor blood [2,3]. The concept of PBM is well established in surgical patients, demonstrating significant reductions in blood usage, yet with improved patient outcomes and reduced cost [4]. However, 52%–65% of recipients of allogeneic blood transfusion are medical patients with hematologic and non-hematologic malignancies, acute gastrointestinal bleed, renal failure, and other chronic disorders, etc [5]. In contrast to surgical patients, PBM is less developed in medical patients, in particular with hematologic/oncologic diseases, by whom a significant percentage of the blood is consumed [3,5]. This review will discuss how to integrate the concept of PBM into medical conditions including acute coronary syndrome (ACS), heart failure, chronic kidney disease (CKD), chemotherapy-induced anemia (CIA), and hematologic malignancies, in the aspect of red blood cell (RBC). Review
PDK4 regulates human cardiomyocyte viability, apoptosis, and inflammatory response via inhibiting TGF-β/Smad signaling pathway

Objective: This study aimed to explore the impact of PDK4 on cardiomyocyte viability, apoptosis, and inflammatory response in patients with atrial fibrillation (AF) by targeting the TGF-β/Smad pathway. Methods: A total of 121 patients with AF and 32 controls with sinus rhythm (SR) were included in this study. Color sonography was used to detect the cardiac diameter of ventricles and atria. Left atrial appendages from patients were collected during the cardiac valvular replacement operation, and cardiomyocytes were separated and purified for subsequent experiments. The cardiomyocytes from AF patients were treated with PDK4 shRNA or TGF-β/Smad pathway inhibitor (LY2157299) and a negative control group (cardiomyocytes transfected with shRNA scrambled sequence) was set up. Masson staining was used to observe the degree of fibrosis in left atrial appendage. qRT-PCR and Western blot were performed to determine the expressions of related mRNA and protein. Cardiomyocyte viability and apoptosis were measured by CCK-8 assay and flow cytometry, respectively. Results: Compared with the SR group, the patients with AF had higher values of left ventricular end-diastolic diameter, left ventricular end-systolic dimension, and left atrial end-diastolic diameter. Moreover, PDK4 expression and TGF-β/Smad pathway were significantly activated in the myocardial tissues of patients with AF. After comparison with the negative control group, we found that PDK4 knockdown could inhibit TGF-β/Smad pathway, promote Cyclin D1 and Bcl-2 protein expressions and cell viability, down-regulate Bax and Caspase-3 protein expressions, and inhibit cell apoptosis. Conclusion: Silencing PDK4 can promote cardiomyocyte viability in AF patients and suppress cell apoptosis and the expression of inflammatory factors by inhibiting the TGF-β/Smad pathway.
Speech Synthesis for Punjabi Language Using Festival

: Processing of digital speech plays an important role in modern speech communication research and applications. The main propose of digital speech is communication which means transmission of messages between human and computer systems. The process of converting the written text into speech is called Text-to-Speech (TTS) and the generation of “synthetic” speech is known as Speech Synthesis. This paper discusses the development of Text-to-Speech system using festival framework for Punjabi language which is in Gurumukhi script. Since festival uses concatenation approach, diphone has been chosen as the basic unit of concatenation for speech synthesis. We defined the phone set and possible number of diphones for Punjabi language. Apart from that configuration of Festival framework on Ubuntu 14.04 Linux is discussed. Recording of nonsense word has been done from which the diphones are extracted for concatenation to produce speech. The aim of this paper is to give the details regarding the manipulations of some files required to add some new language in festival.
A Parameter free Clustering of Density Based Algortihm

— Clustering is a kind of unsupervised learning process in data mining and pattern recognition, most of the clustering algorithms are sensitive to their input parameters. So it is necessary to evaluate results of the clustering algorithms. It is difficult to define which clustering designs are acceptable hence several clustering validation measures are developed. In the present paper we have a study of implementation on some of very common data sets with internal index compared to our new and improved parameter free Density Based Clustering with the help of differential evolution. Density based clustering methods are being used for clustering spatial databases with noise. Density Clustering of Spatial Data and its Application with noise (DBSCAN) can discover clusters of arbitrary shapes and sizes effectively with the help of Eps(radius of the cluster) and MinPts (minimum number of points to be inside the cluster). The value of these parameters is very important in determining clustering results as the output varies significantly with the little changes in the values and is also very hard to determine these parameters a priori. In this paper we present a new algorithm named Parameter free Density Based Algorithm using Differential evolutions, which uses the combination of analytical ways to determine the efficient values of Eps and Minpts using Differential Evolution Method. The Experimental results show that our algorithm is precise in selecting the parameters and efficient.
EFFECTS OF DIFFERENT ORGANIC WASTES ON THE GROWTH, YIELD, MARKET QUALITY AND PROTEIN CONTENT OF LENTINUS SQUARROSULUS (MONT.) SINGER, AN EDIBLE NIGERIAN MUSHROOM

Four organic wastes; mahogany (Khaya ivorensis) sawdust (MSD), Gmelina aborea sawdust (GSD), oil palm fruit fibre (OPFF) and oil palm empty fruit bunch (OPEFB) were evaluated for their effects on growth, yield, quality and protein content of Lentinus squarrosulus (Mont.) Singer. Plastic bag technology was used with treatments replicated ten times and arranged using a completely randomized design. The quality of the harvested mushrooms was evaluated on the basis of four pileus diameter size groups (>7 cm, 5-7 cm, 3-5 cm, <3 cm) and a deformed group; while their protein analyses were carried out using Kjeldahl’s method. Results on mushroom growth showed that oil palm fruit fibre (OPFF) took the least time for full mycelial colonization and the longest time occurred on Gmelina sawdust (GSD). Analysis of variance showed that there were significant differences (P < 0.05) in the time required for primordia initiation of mushrooms grown on oil palm empty fruit bunch (OPEFB), oil palm fruit fibre (OPFF) and Gmelina sawdust (GSD). Results on mushroom yield revealed that mean fresh weights of harvested mushrooms varied from 4.12 ± 0.16 g on oil palm empty fruit bunch (OPEFB) to 16.05 ± 0.68 g on mahogany sawdust (MSD). There were significant differences (P < 0.05) in the biological efficiency of mushrooms grown on mahogany sawdust (MSD), Gmelina sawdust (GSD) and oil palm empty fruit bunch (OPEFB). Mahogany sawdust produced the highest quality mushrooms, with 26% in the >7 cm group while Gmelina sawdust (GSD) and oil palm empty fruit bunch (OPEFB) had none in the same quality group. The percentage protein content of harvested mushrooms ranged from 13.27% for mushrooms produced from mahogany sawdust (MSD) to 27.42% for those grown on oil palm fruit fibre (OPFF). The above findings reveal the possibility of commercial production of high quality L. squarrosulus on oil palm fruit fibre (OPFF) and mahogany sawdust (MSD) while oil palm fruit fibre (OPFF) is recommended as the best substrate for spawn production among the various organic wastes used in this study.
Running head : RERPS II 1 Regression-based estimation of ERP waveforms : II . Non-linear effects , overlap correction , and practical considerations

A companion paper introduced the rERP framework, which re-casts traditional ERP averaging as a special case of a more flexible regression-based approach to estimating ERP waveforms. Here, we build on this foundation, showing how rERP analysis can also handle the estimation of non-linear effects (a generalization of both the well-known approach of dichotomizing continuous covariates, and also of the ERP image technique), and can disentangle overlapping ERPs to temporally adjacent stimuli. We then consider how the use of rERPs impacts on other parts of the EEG analysis pipeline, including baselining, filtering, significance testing, and artifact rejection, and provide practical recommendations. Free software implementing these techniques is available.
MODE OF TERM SINGLETON BREECH DELIVERY

Mode of term singleton breech delivery is an area of intense controversy. This is a prospective comparative study conducted in tow major hospitals Khartoum Teaching Hospital and Omdurman maternity hospital in the period between 1 of September 2002 and 30 of November 2002. In this study tow group were selected, 50 cases planned for vaginal delivery and 50 cases planned for cesarean section and the two groups were compared in terms of maternal and fetal outcome. Special selection criteria was considered (Term, Singleton and Viable fetus). The objectives of this study were to evaluate the impact of mode of term singleton breech livery in the maternal and fetal outcome, and to \ evaluate management of breech presentation in our hospitals. Regarding maternal mortality and morbidity, no maternal death is reported in either of the two groups, however, maternal morbidity is found to be more in C/S group, and moreover the complication reported in C/S group is more serious. Concerning fetal mortality and morbidity, the study reported more fetal morbidity in VD group. Fetal birth trauma (5%) in VD group , while (1%) in C/S group , Apgar score after 1 minute < 7 is found in 8% of cases in vaginal delivery , while 4% in cesarean section , and regarding fetal mortality there were 3 death in VD group (6%) compared with one death in C/S group (2%) . Fetal mortality after exclusion of lethal congenital anomalies was (4%). The study concluded that in appropriately selected and managed cases, and with informed consent, both planned C/S and vaginal breech delivery remain appropriate and reasonable management options. This study recommended careful selection of the cases regarding the mode of delivery, to minimize maternal and perinatal morbidity and mortality. The study also recommended providing baby care unit in all maternity hospitals, training programs for adequate dealing with breech delivery, and establishment of recording system and auditing in our hospitals for future studies in this issue.
Demand Response in a Residential Building using Multi Agent Control System

This paper proposes a multi agent system (MAS) based demand response implementation for a residential building. The main objective of the proposed system is to facilitate the customers to take part in supply demand management through the demand response. For this, the system offers demand response in two ways such as connecting/disconnecting the loads from power outlets and controlling the characteristics of infrared radiation (IR) controlled loads. Three hardware modules are designed for power consumption monitoring, room temperature monitoring, on/off switch controlling, and IR load controlling. A multi agent-based control system is proposed and developed to control the loads and a sniffer agent output is shown to demonstrate the commands within the system.
Rebound effect on Charged Based Bio-TFETs for different biomolecules

In this work, the charged biomolecules with different permittivity (ε) localized on the drain underlap region of a double gate (DG) N-type tunneling field effect transistor (nTFET) for biosensing purpose (Bio- TFET) is presented. The fixed charges are localized at the interface between the biomaterial and the silicon channel. The main studied parameter of the charged Bio- TFET is the sensitivity. In the charge range studied, the results show that the device presents higher sensitivity for lower dielectric constant biomolecules (e.g., thiol linkers and biotin) and it was also observed a rebound effect on the drain current (and sensitivity) for high concentration (> 1×1012 cm−2) of positive fixed charges. This rebound effect is caused by the shift of the electric field peak from the drain/channel to the gate/biomaterial regions.
Highly-Integrated Radar Transceiver with 2 TX and 4 RX Channels for Range, Azimuthal and Polar Angle Measurements

A highly-integrated V-band 6-channel radar sensor implemented in Silicon Germanium BiCMOS technology is presented in this paper. The transceiver, which is composed of two transmit as well as four receive channels, is very suitable for multiple-input multiple-output (MIMO) radar systems and can be complemented by 2-D antenna array configuration to enable the measurements of the 2-D and 3-D positions of objects. The fabricated transceiver chip was used to build a MIMO radar system that achieves an azimuthal and a polar angular resolution of 28.6° and 57.3°, respectively.
Optimizing the Number of Steps and the Noise in Staircase APDs with Ternary III - V Semiconductor Alloys

We report simulation results for gain and noise in avalanche photodiodes fabricated using heterojunctions of III-V compound semiconductors. We employ a recently developed nonlocal model for impact ionization. The model has been calibrated and validated on devices fabricated and measured in our labs and data from the literature. The model is then used to explore the design trade-offs related to the number of conduction band steps in staircase APDs for X-ray detection based on the GaAs/AlGaAs material system.