Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison and interaction of morphine and CB1 agonist conditioned place preference in the rat model of early life stress

Early life stress (ELS) disrupts brain development and subsequently affects physical and psychological health. ELS has been associated with an increased risk of relapse and inadequate treatment response in addicted patients. The current study was designed to find the effect of ELS on the rewarding effect of morphine and cannabinoid and their interaction. Pregnant female Wistar rats were used in this study. On postnatal day 2 (PND2), pups were separated from their mothers for 3 hr daily. This procedure was repeated every day at the same time until PND 14. The control group was kept in the standard nesting way with their mothers. The adult male offspring of maternal separated (MS) and standard nested (SN) rats were used. Using conditioned place preference task (CPP), the rewarding effect of morphine (0.75, 1.25, 2.5, and 5 mg/kg) was evaluated in both MS and SN groups. Besides, the rewarding effect of cannabinoids was investigated using the administration of CB1 receptor agonist (ACPA, 0.25, 0.5, 1 µg/rat) and inverse agonist (AM‐251, 30, 60, and 90 ng/rat) in the nucleus accumbens (NAc). To evaluate the interaction between NAc cannabinoidergic system and morphine, the noneffective dose of ACPA and AM‐251 were administered with a noneffective dose of morphine (0.75 mg/kg) on both MS and SN animals. Obtained results indicated that MS groups had a leftward shift in the rewarding effect of morphine and conditioned with low doses of morphine. However, they had a rightward shift in the rewarding effect of cannabinoids. In addition, coadministration of noneffective doses of morphine and ACPA potentiate conditioning in both MS and SN groups. Previous evidence shows that ELS induced changes in the brain, especially in the reward circuits. Here, we demonstrated that MS animals are more sensitive to the rewarding effect of morphine compared with SN animals. In addition, ELS disrupts the cannabinoid system and affect the rewarding effect of cannabinoids.
Ratiometric delivery of cisplatin and doxorubicin using tumour-targeting carbon-nanotubes entrapping platinum(IV) prodrugs

Combination therapy is an effective strategy to enhance the efficacy of single-agent chemotherapy and delay onset of chemoresistance. However, differences in the pharmacokinetic profiles of the drug constituents can complicate the implementation of combination regimens in a clinical setting. Nanomaterials can overcome these limitations by offering a unified platform for targeted and synchronous delivery of multiple drugs, although exact ratiometric loading cannot be assured using conventional encapsulation techniques. An approach was conceived with the goal of delivering exact stoichiometric proportions of cisplatin and doxorubicin against endometrial adenocarcinoma using tumour-targeting multi-walled carbon nanotubes entrapping an inert Pt(IV) prodrug. Activation of the Pt(IV) prodrug after cell entry, synchronously releases molar equivalents of hydrophilic cisplatin and doxorubicin from the hydrophobic confines, thereby achieving ratiometric delivery of these mechanistically-complementary drug entities.
Hard x-ray emission spectroscopy: a powerful tool for the characterization of magnetic semiconductors

This review aims to introduce the x-ray emission spectroscopy (XES) and resonant inelastic x-ray scattering (RIXS) techniques to the materials scientist working with magnetic semiconductors (e.g. semiconductors doped with 3d transition metals) for applications in the field of spin-electronics. We focus our attention on the hard part of the x-ray spectrum (above 3 keV) in order to demonstrate a powerful element- and orbital-selective characterization tool in the study of bulk electronic structure. XES and RIXS are photon-in/photon-out second order optical processes described by the Kramers–Heisenberg formula. Nowadays, the availability of third generation synchrotron radiation sources permits applying such techniques also to dilute materials, opening the way for a detailed atomic characterization of impurity-driven materials. We present the Kβ XES as a tool to study the occupied valence states (directly, via valence-to-core transitions) and to probe the local spin angular momentum (indirectly, via intra-atomic exchange interaction). The spin sensitivity is employed, in turn, to study the spin-polarized unoccupied states. Finally, the combination of RIXS with magnetic circular dichroism extends the possibilities of standard magnetic characterization tools.
Nosocomial infection of COVID-19: A new challenge for healthcare professionals (Review)

Nosocomial infections, also known as hospital-acquired infections, pose a serious challenge to healthcare professionals globally during the Coronavirus disease 2019 (COVID-19) pandemic. Nosocomial infection of COVID-19 directly impacts the quality of life of patients, as well as results in extra expenditure to hospitals. It has been shown that COVID-19 is more likely to transmit via close, unprotected contact with infected patients. Additionally, current preventative and containment measures tend to overlook asymptomatic individuals and superspreading events. Since the mode of transmission and real origin of COVID-19 in hospitals has not been fully elucidated yet, minimizing nosocomial infection in hospitals remains a difficult but urgent task for healthcare professionals. Healthcare professionals globally should form an alliance against nosocomial COVID-19 infections. The fight against COVID-19 may provide valuable lessons for the future prevention and control of nosocomial infections. The present review will discuss some of the key strategies to prevent and control hospital-based nosocomial COVID-19 infections.
Thermal Tuning of Planar Bragg Gratings in Silicon-on-Insulator Rib Waveguides

The Bragg grating forms an essential element in optical telecommunication and sensing applications. In the case of integrated optics, it is necessary to incorporate the grating into a waveguide during device fabrication. Specifically for silicon photonics this process is deemed necessary to be compatible with standard processing technology. We have recently developed a method for the fabrication of Bragg gratings via self-ion implantation of silicon-on-insulator (SOI) waveguides, such that the surface of the waveguide remains planar. This has significant benefits for a number of devices especially those requiring subsequent wafer bonding. Here we report work that shows the ability of these devices to be thermally tuned. As the waveguides are heated, a positive shift in the resonant wavelength is induced due to the temperature dependence of the index of refraction and thermal expansion of the silicon. The observed shift is 0.080 nm/degree, in good agreement with theoretical prediction. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
Phosphorylierungsmittel — Bauprinzip und Reaktionsweise

Phosphorylierungsmittel ubertragen die Gruppe (HO)2P(O) – in freier oder veresterter Form. Zahlreiche Phosphorylierungsmittel lassen sich als P–XYZ-Systeme auffassen; dabei mus das Element oder die Gruppe Z in der Lage sein, die Elektronen der P–X-Bindung aufzunehmen. Auser einer Systematik der P–XYZ-Verbindungen werden viele Beispiele fur Phosphorylierungen aus Chemie und Biochemie angegeben.
Object-mediated overwriting across saccades

How are visual sensory representations that are acquired peripherally from a saccade target related to sensory representations generated foveally after the saccade? We tested the hypothesis that, when the two representations are perceived to belong to the same object, the post-saccadic value tends to overwrite the pre-saccadic value. Participants executed a saccade to a colored target object, which sometimes changed during the saccade by ±15°, 30°, or 45° in color space. They were post-cued to report either the pre-saccadic or post-saccadic color in a continuous report procedure. Substantial overwriting of the pre-saccadic color by the post-saccadic color was observed. Moreover, the introduction of a brief post-saccadic blank interval (which disrupted the perception of object correspondence) led to a substantial reduction in overwriting. The results provide the first direct evidence for an object-mediated overwriting mechanism across saccades, in which post-saccadic values automatically replace pre-saccadic values.
Comprehensive analysis of prognostic immune-related genes in the tumor microenvironment of colorectal cancer

In this study, we used the ESTIMATE algorithm to analyze clinical data and transcriptome profiles of 1635 colorectal cancer (CRC) samples from the Gene Expression Omnibus and The Cancer Genome Atlas databases and identify prognostic immune-related genes (IRGs). We identified 941 differentially expressed (4 downregulated and 937 upregulated) genes by comparing samples with high and low immune, stromal scores and tumor purity. LASSO Cox regression analyses showed that the risk score based on a ten-IRG signature was an independent prognostic factor in CRC. The nomogram with pathological stages (TNM) and the ten-IRG signature showed a C-index of 0.769 (95% CI, 0.717-0.821), and area under ROC curve values of 0.788, 0.782 and 0.789 for 1-, 3-, and 5-year OS, respectively. TIMER database analysis showed positive correlation between the ten prognostic IRGs and the levels of tumor-infiltrated immune cells, including CD4+ and CD8+ T cells, macrophages, neutrophils, and dendritic cells. These findings demonstrate that this novel ten-IRG signature correlates with the pathological stages and the levels of multiple tumor-infiltrated immune cell types. This makes the ten-IRG signature a potential prognostic factor for CRC patients.
Hermansky–Pudlak syndrome pulmonary fibrosis: a rare inherited interstitial lung disease

Pulmonary fibrosis is a progressive interstitial lung disease of unknown aetiology with a poor prognosis. Studying genetic diseases associated with pulmonary fibrosis provides insights into the pathogenesis of the disease. Hermansky–Pudlak syndrome (HPS), a rare autosomal recessive disorder characterised by abnormal biogenesis of lysosome-related organelles, manifests with oculocutaneous albinism and excessive bleeding of variable severity. Pulmonary fibrosis is highly prevalent in three out of 10 genetic types of HPS (HPS-1, HPS-2 and HPS-4). Thus, genotyping of individuals with HPS is clinically relevant. HPS-1 tends to affect Puerto Rican individuals due to a genetic founder effect. HPS pulmonary fibrosis shares some clinical features with idiopathic pulmonary fibrosis (IPF), including dyspnoea, cough, restrictive lung physiology and computed tomography (CT) findings of fibrosis. In contrast to IPF, HPS pulmonary fibrosis generally affects children (HPS-2) or middle-aged adults (HPS-1 or HPS-4) and may be associated with ground-glass opacification in CT scans. Histopathology of HPS pulmonary fibrosis, and not IPF, shows vacuolated hyperplastic type II cells with enlarged lamellar bodies and alveolar macrophages with lipofuscin-like deposits. Antifibrotic drugs approved as treatment for IPF are not approved for HPS pulmonary fibrosis. However, lung transplantation has been performed in patients with severe HPS pulmonary fibrosis. HPS pulmonary fibrosis serves as a model for studying fibrotic lung disease and fibrosis in general. Hermansky–Pudlak syndrome (HPS) is an autosomal recessive disorder characterised by highly prevalent pulmonary fibrosis. HPS pulmonary fibrosis is a rare inherited interstitial lung disease that can serve as a model for studying fibrotic lung disorders. https://bit.ly/2ZWFR2Y
Salivary Biomarkers of Stress, Anxiety and Depression

Stress, anxiety and depressive disorders are often characterized by the activation of the stress axis, which results in similar symptoms at some point in these disorders. These disorders are closely related to each other—they occur simultaneously or follow one another. The diagnosis of stress, anxiety and depression is not a perfect procedure currently—it is based on patient observation and an interview with the patient and their family. There are no laboratory tests that would dispel the doubts of the doctor making the diagnosis and allow the appropriate treatment to be implemented as soon as possible. Therefore, this study will review the components of saliva that could be helpful in the quick diagnosis of stress, anxiety and/or depression. Such potential salivary biomarkers could also be useful in monitoring the effectiveness of pharmacological treatment prescribed by a psychiatrist. The following are promising salivary biomarkers of stress, anxiety or depression: cortisol, immunoglobulin A (sIgA), lysozyme, melatonin, α-amylase (sAA), chromogranin A (CgA) and fibroblast growth factor 2 (FGF-2). To the best valuable potential salivary markers of stress, we can include cortisol, lysozyme, sAA and CgA. To differentiate depression from stress, salivary cortisol and melatonin can be helpful. Fluctuations in the concentrations of the above-mentioned substances in saliva indicate a particularly strong relationship with typical human psychological problems, such as stress, depression or anxiety.
Association of High-Density Lipoprotein Cholesterol Variability and the Risk of Developing Parkinson Disease

Objective To investigate the longitudinal association among high-density lipoprotein cholesterol (HDL-C) level, HDL-C variability, and the risk of developing Parkinson disease (PD). Methods We conducted a nationwide, population-based cohort study. We included 382,391 patients aged ≥65 years who underwent at least 3 health examinations provided by the Korean National Health Insurance System from 2008 to 2013 and followed up until 2017. Individuals with a history of PD and missing values were excluded (n = 1,987). We assessed HDL-C variability using 3 indices, including variability independent of the mean (VIM). A multivariate-adjusted Cox proportional hazards regression analysis was performed. Results Among the 380,404 participants, 2,733 individuals were newly diagnosed with PD during a median follow-up period of 5 years. The lowest quartile (Q1) group of baseline HDL-C and mean HDL-C was associated with increased PD incidence as compared with the highest quartile (Q4) group (adjusted hazard ratio [aHR], 1.20; 95% confidence interval [CI], 1.08–1.34; and aHR, 1.16; 95% CI, 1.04–1.30, respectively). The Q4 group of HDL-C variability (VIM) was associated with increased PD incidence compared to the Q1 group (aHR, 1.19; 95% CI, 1.06–1.33). The group with the Q1 of baseline HDL-C and with the Q4 of HDL-C variability had the highest risk of PD incidence (aHR, 1.6; 95% CI, 1.31–1.96). Conclusion Lower HDL-C level and greater HDL-C variability were associated with a higher incidence of PD.
Effects of Fulvic Acid on Ensiling Properties of Alfalfa (Medicago Sativa L.)

Many silage additives have been developed for use in silage fermentation. This research was carried out to determine the effects of organic liquid based fulvic acid which derive from Isparta Keciborlu district, on the silage characteristics and the effects on the nutrient contents of the alfalfa silage ensiling to laboratory conditions. Fulvic acid based organic liquid was applied at 0 % (control), 0,25 %, 0,50 %, 1 % levels to fresh alfalfa which dry matter content is 21 %. Fresh materials were ensiled as three repeat in 5 kg anaerobic jars. In this research, nutrition value of alfalfa silage with fulvic acid was determined after ensiling. ME and NEL contents were calculated on nutrient contents in alfalfa silages. Adding of fulvic acid based organic liquid was determined there is statistically not significant on chemical composition, energy values and quality properties in silages but pH value was decreasing 5.20 to 4.82 and Flieg point was increasing 36.82 to 52.73 depending on pH. According to our result 1 % fulvic acid is an useful material for silage and can be ensiled successfully with alfalfa.
Detection of Silica Particles in Lung Tissue by Environmental Scanning Electron Microscopy

For pathologists, pneumologists, and occupational and environmental physicians it is relevant to know silica levels in lung tissue to better define limits of exposure. Environmental Scanning Electron Microscopy (ESEM) has been employed to detect silica particles and to compare silica levels in subjects with and without Lung Cancer (LC). We investigated 25 paraffin-embedded tissue samples of patients with LC adenocarcinoma, and 20 fresh samples of subjects without LC deceased for extra-pulmonary diseases. Silica levels were quantified considering the Number of Spots of silica particles (NS), and the Number of Positive Zones (NPZ) in which there was at least one spot. Levels of NS and NPZ were assessed with Poisson-type regression models, and in two samples of silica-exposed workers with LC the performance of models were evaluated. LC patients displayed higher silica levels, as compared to controls; smoking, age and gender had no significant effects on this relationship. Values of NS and NPZ for the exposed workers were in agreement with model estimates. The fitted model between NS and NPZ might be useful in evaluating new observations and in the development of threshold limit values of silica in biological tissues. ESEM is a rapid, simple and valid tool for the determination of silica levels in lung tissues.
Epidermal Club Cells in Fishes: A Case for Ecoimmunological Analysis

Epidermal club cells (ECCs), along with mucus cells, are present in the skin of many fishes, particularly in the well-studied Ostariophysan family Cyprinidae. Most ECC-associated literature has focused on the potential role of ECCs as a component of chemical alarm cues released passively when a predator damages the skin of its prey, alerting nearby prey to the presence of an active predator. Because this warning system is maintained by receiver-side selection (senders are eaten), there is want of a mechanism to confer fitness benefits to the individual that invests in ECCs to explain their evolutionary origin and maintenance in this speciose group of fishes. In an attempt to understand the fitness benefits that accrue from investment in ECCs, we reviewed the phylogenetic distribution of ECCs and their histochemical properties. ECCs are found in various forms in all teleost superorders and in the chondrostei inferring either early or multiple independent origins over evolutionary time. We noted that ECCs respond to several environmental stressors/immunomodulators including parasites and pathogens, are suppressed by immunomodulators such as testosterone and cortisol, and their density covaries with food ration, demonstrating a dynamic metabolic cost to maintaining these cells. ECC density varies widely among and within fish populations, suggesting that ECCs may be a convenient tool with which to assay ecoimmunological tradeoffs between immune stress and foraging activity, reproductive state, and predator–prey interactions. Here, we review the case for ECC immune function, immune functions in fishes generally, and encourage future work describing the precise role of ECCs in the immune system and life history evolution in fishes.
The Role of the Histone Methyltransferase PfSET10 in Antigenic Variation by Malaria Parasites: a Cautionary Tale

The identification of specific epigenetic regulatory proteins in infectious organisms has become a high-profile research topic and a focus for several drug development initiatives. However, studies that define specific roles for different epigenetic modifiers occasionally report differing results, and we similarly provide evidence regarding the histone methyltransferase PfSET10 that is in stark contrast with previously published results. ABSTRACT The virulence of the malaria parasite Plasmodium falciparum is due in large part to its ability to avoid immune destruction through antigenic variation. This results from changes in expression within the multicopy var gene family that encodes the surface antigen P. falciparum erythrocyte protein one (PfEMP1). Understanding the mechanisms underlying this process has been a high-profile research focus for many years. The histone methyltransferase PfSET10 was previously identified as a key enzyme required both for parasite viability and for regulating var gene expression, thus making it a prominent target for developing antimalarial intervention strategies and the subject of considerable research focus. Here, however, we show that disruption of the gene encoding PfSET10 is not lethal and has no effect on var gene expression, in sharp contrast with previously published reports. The contradictory findings highlight the importance of reevaluating previous conclusions when new technologies become available and suggest the possibility of a previously unappreciated plasticity in epigenetic gene regulation in P. falciparum. IMPORTANCE The identification of specific epigenetic regulatory proteins in infectious organisms has become a high-profile research topic and a focus for several drug development initiatives. However, studies that define specific roles for different epigenetic modifiers occasionally report differing results, and we similarly provide evidence regarding the histone methyltransferase PfSET10 that is in stark contrast with previously published results. We believe that the conflicting results, rather than suggesting erroneous conclusions, instead reflect the importance of revisiting previous conclusions using newly developed methodologies, as well as caution in interpreting seemingly contrary results in fields that are known to display considerable plasticity, for example metabolism and epigenetics.
Biological Control and Mitigation of Aflatoxin Contamination in Commodities

Aflatoxins (AFs) are toxic secondary metabolites produced mostly by Aspergillus species. AF contamination entering the feed and food chain has been a crucial long-term issue for veterinarians, medicals, agroindustry experts, and researchers working in this field. Although different (physical, chemical, and biological) technologies have been developed, tested, and employed to mitigate the detrimental effects of mycotoxins, including AFs, universal methods are still not available to reduce AF levels in feed and food in the last decades. Possible biological control by bacteria, yeasts, and fungi, their excretes, the role of the ruminal degradation, pre-harvest biocontrol by competitive exclusion or biofungicides, and post-harvest technologies and practices based on biological agents currently used to alleviate the toxic effects of AFs are collected in this review. Pre-harvest biocontrol technologies can give us the greatest opportunity to reduce AF production on the spot. Together with post-harvest applications of bacteria or fungal cultures, these technologies can help us strictly reduce AF contamination without synthetic chemicals.
Experimental Study of the Mechanism of Thermoluminescence in Irradiated Sodium Chloride

The general configuration of the emission spectrum of synthetic NaCl crystals which had been irradiated with 7 × 105r of x‐rays at room temperature and were then permitted to luminesce at 500°K was determined, and bands were located with maxima at 362, 418, 432, and 525 millimicrons. The glow curves for NaCl crystals irradiated with x‐rays or ultraviolet light were investigated, and eight bursts of light (glow peaks) were found with peaks at temperatures between 334 and 625°K. The relative contribution of the different bursts to the total glow curve was determined as a function of the amount of x‐ray irradiation. The activation energies for five of the bursts were measured and in each case turned out to be about 1.25 ev. It is suggested that these results indicate that the thermoluminescence takes place by means of a two‐stage process of the following sort. The first stage, which is the same for all the bursts and is thermally activated with the measured activation energy, takes place when trapped electro.