Miracle-dz/UniFAR-FTUs huggingface dataset:
Long non-coding RNA colon cancer-associated transcript 2 may promote esophageal cancer growth and metastasis by regulating the Wnt signaling pathway

The aim of this study was to investigate how long non-coding (lnc)RNA colon cancer-associated transcript 2 (CCAT2) regulates the proliferation, invasion and metastasis of esophageal cancer cells via the Wnt signaling pathway. The expression of lncRNA CCAT2 was quantified by reverse transcription-quantitative PCR in four esophageal cancer cell lines (Eca-109, EC9706, KYSE150 and TE-1) and normal human esophageal epithelial cells (HEECs). The effect of silencing CCAT2 (si-CCAT2) and inhibiting Wnt signaling (using the inhibitor FH535) on the proliferation, migration and invasion of Eca-109 cells was measured by MTT, wound-healing and Transwell invasion assays. Flow cytometry was used to evaluate apoptosis in si-CCAT2 Eca-109 cells. The expression of β-catenin and proliferating cell nuclear antigen (PCNA) proteins was detected by immunohistochemistry. The pro-apoptotic protein Bax, cyclin D1 and Wnt target proteins, including c-Myc and adenomatous polyposis coli (APC), were detected by western blotting. LncRNA CCAT2 was highly expressed in the four esophageal cancer cell lines compared with the HEEC cells. The expression of CCAT2 was significantly decreased in si-CCAT2 Eca-109 cells. Treatment with si-CCAT2 and FH535 alone or in combination significantly inhibited the proliferation, migration and invasion of Eca-109 cells. The treatments also promoted apoptosis, upregulated the expression of Bax and APC proteins, and downregulated β-catenin, PCNA, cyclin D1 and c-Myc proteins. In summary, lncRNA CCAT2 is upregulated in esophageal cancer cells and the knockdown of lncRNA CCAT2 inhibits their proliferation, migration and invasion via the Wnt signaling pathway.
transmembrane segments temperature dependence: NH2-terminal Determinants of cardiac Na+/Ca2+ exchanger

You might find this additional information useful. This article cites 32 articles, 20 of which you can access free at: http://ajpcell.physiology. org/cgi/content/full/283/2/C512#BIBL Updated information and services including high-resolution figures, can be found at: http://ajpcell.physiology. org/cgi/content/full/283/2/C512 Additional material and information about AJP - Cell Physiology can be found at: http://www.the-aps.org/publications/ajpcell This information is current as of January 7, 2008 . € American Physiological Society. ISSN: 0363-6143, ESSN: 1522-1563. Visit our website at http://www.the-aps.org/.
Renal biopsy findings during and after pregnancy

Background Chronic kidney disease is estimated to affect up to 6% of women of reproductive age. Maternity care represents an opportunity for early diagnosis but there is limited understanding of chronic kidney disease aetiology occurring in or revealed by pregnancy. Methods A retrospective evaluation of renal biopsies during and after pregnancy between 2000 and 2015 was undertaken. A large academic health centre pathology database was searched for free text pregnancy-related terms, restricted to typology code 71000 (kidney). Indications and findings of postpartum renal biopsies were reviewed. Results Sixty-three renal biopsy reports were identified. Of 45 biopsies performed postpartum, 34 (75.6%) investigated persistent postpartum proteinuria. 20/34 (70.6%) of these biopsies yielded a primary renal disease, and 6/34 (17.6%) women had progressed to end stage renal disease at latest follow-up. Conclusion Renal biopsy findings of women investigated for persistent postpartum proteinuria revealed a high incidence of histological diagnosis of de novo renal disease.
Evaluation of selected mechanisms of immune tolerance in psoriasis

Introduction Psoriasis is an autoimmune disease with an excessively aberration of the Th17/Treg balance and deficiency of anti-inflammatory cytokines. Aim Evaluation of Treg markers expression in the lesional and perilesional psoriatic skin and serum anti-inflammatory cytokines in male psoriatic patients compared to healthy men. Material and methods Treg markers (FoxP3+, CD4, CTLA-4, CD25/IL-2R, CD39/ENTPD1, IL-7R/CD127, CD3) and tissue expression of protective cytokines (IL-10, IL-35, TGF-β) in the lesional and perilesional psoriatic skin from 33 male patients compared to 6 healthy skin samples were evaluated by immunohistochemistry. ELISA was used to assess serum IL-10, IL-35 and TGF-β levels. Results The serum levels of IL-35, IL-10 and TGF-β1 were higher in psoriatic patients than in controls but without any statistically significant relationship with PASI. The expressions of IL-35, CD4, IL-10, TGF-β1, CD3, FOXP3 and CD25/IL-2R were varied in different experimental groups (p < 0.05). The level of IL-35 was the lowest in psoriatic lesions (p < 0.05) compared to perilesional skin and to controls. CD4, IL-10 and TGF-β1 expressions were higher (p < 0.05) in perilesional skin than in lesions. TGF-β1 expression was decreased in psoriatic lesions compared to controls (p < 0.05). CD25/IL2R expression was increased in healthy skin compared to psoriatic skin (p < 0.05). FOXP3 expression was elevated in psoriatic skin compared to healthy and perilesional one. There was no difference between experimental groups in CTLA-4, IL7R/CD127 and CD39/ENTPD1 expression. Conclusions The differences between the levels of protective cytokines and expression of Treg markers might explain the inflammation development in psoriasis.
Advanced flow visualization

Flow visualization has been playing a substantial role in many engineering and scientific applications, such as automotive industry, computational fluid dynamics, chemical processing, and weather simulation and climate modelling. Many methods have been proposed in the past decade to visualize steady and time-varying flow fields, in which textures-based and geometry-based visualization are widely used to explore the underlying fluid dynamics. This dissertation presents a view-dependent flow texture algorithm, an illustrative streamline placement algorithm on two-dimensional vector fields, and an image-based streamline placement algorithm on three-dimensional vector fields. Flow texture, generated through convolution and filtering of texture values according to the local flow vectors, is a dense representation of the vector field to provide global information of the flow structure. A view-dependent algorithm for multi-resolution flow texture advection on two-dimensional structured rectilinear and curvilinear grid is presented. By using an intermediate representation of the under-lying flow fields, the algorithm can adjust the resolutions of the output texture on the fly as the user zooms in and out of the field, which can avoid aliasing as well as ensure enough detail. Geometry-based methods use geometries, such as lines, tubes, or balls, to represent the motion paths advected from the vector fields. It provides a sparse representation and an intuitive visualization of flow trajectory. For two-dimensional vector fields, a streamline placement strategy is presented to generate representative and illustrative streamlines, which can effectively prevent the visual overload by emphasizing the essential and deemphasizing the trivial or repetitive flow patterns. A user study is performed to quantify the effectiveness of this visualization algorithm, and the results are provided. For three-dimensional vector fields, an image-based streamline seeding algorithm is introduced to better display the streamlines and reduce visual cluttering in the output images. Various effects can be achieved to enhance the visual understanding of three-dimensional flow lines.
A STUDY TO CORRELATE VSS AND VHQ IN PATIENTS WITH VERTIGO DUE TO PERIPHERAL VESTIBULAR DISORDERS

There are ample of studies on use of VSS in order to find out the severity of vertigo symptoms, also studies portray the usefulness of VHQ to study the impact of vertigo on lifestyle; however, there is dearth of information on whether there is any correlation between the vertigo symptoms and affection on quality of life (QOL). Hence, the need arises to find out the correlation between VSS and VHQ for the better, documentation of symptoms and affection on QOL, as it will benefit treatment efficacy. METHODS: Sample size was 40. All the patients were given two questionnaires VSS and VHQ, which are self-administered. RESULTS: Correlation was found between total VSS and its dimensions with VHQ using Karl Pearson correlation coefficient method. Thus, we concluded that increase or decrease in the scores of VSS has an effect on the scores of VHQ.
THU0028 COMPARISON OF IL-17A AND TNF INDUCED CYTOKINE SECRETION BY RHEUMATOID AND PSORIATIC ARTHRITIS SYNOVIAL FIBROBLASTS AND THEIR INHIBITION BY BIOLOGICS

Background Rheumatoid arthritis (RA) and psoriatic arthritis (PsA), although similar in several respects, display clinical as well as therapeutic differences. This includes, for example, the higher effectiveness of the anti-IL17A biologic secukinumab in PsA than in RA patients. Synovial fibroblasts (SF) are one of the key effector cell types in the pathophysiology of RA and PsA. We hypothesized that RASF and PsASF respond differentially to IL-17A and its biologic secukinumab and that this might contribute to the difference seen in the therapeutic response. Objectives To examine the effect of the two cytokines IL-17A and TNF-α on RASF in comparison to PsASF as well as the effect of their corresponding biologics. To analyze the effect of the IL-17A homolog IL-17F. Methods SF were isolated from synovium from PsA or RA patients undergoing surgery. SF from RA and PsA patients were stimulated with recombinant IL-17A, IL-17F and TNF-α alone or with respective combinations. Dose-response curve analysis was performed with IL-17A. The biologics secukinumab and adalimumab were used to block the effects on the SF. As a measure of the proinflammatory response, secretion of the cytokine IL–6 was quantified using an immunoassay. Results IL-6 secretion was induced by IL-17A in RASF as well as PsASF (IL-17A: 13.7-fold ↑ vs 6.9–fold ↑; n=3). Although sharing the same receptor, the IL-17A homolog IL-17F alone caused no induction of IL-6 secretion in SF. However, when combined with TNF-α, both IL-17 isoforms, IL–17A and IL-17F, increased IL-6 secretion due to a strong synergistic effect with TNF-α. RASF (n=3) responded more strongly than PsASF (n=3) to the combined stimulation (IL-17A: 544-fold ↑ vs 127-fold ↑, IL-17F: 54-fold ↑ vs 27-fold ↑). Adalimumab and secukinumab were similarly effective in abolishing the synergistic effect of IL-17A + TNF-α in RASF and PsASF. Conclusion According to our data, the differences in the therapeutic effectiveness of the anti-IL17A biologic secukinumab cannot be attributed to differential SF responses since the response to IL-17A alone and IL-17A together with TNF–α is not stronger for PsASF than for RASF and since secukinumab was similarly effective for both SF types. Furthermore, in a proinflammatory milieu with increased TNF levels, both IL-17A and IL-17F can contribute to promoting inflammation in the pathophysiology of PsA and RA. Acknowledgement Supported by an unrestricted educational grant from Celgene GmbH. Disclosure of Interests Klaus Frommer: None declared, Stefan Rehart: None declared, Ulf Müller-Ladner Grant/research support from: Projekt supported by an unrestricted educational grant from Celgene GmbH. , Elena Neumann: None declared
Study on the Data Mining of Image Storage on the Hadoop Cloud Platform

Along with the rapid development of image acquisition and image storage, a huge number of usable image data are obtained by people, such as satellite remote sensing image data, medical image data, etc. Data mining of images is to analyze these useful images and extract the usable information from them. How to effectively store rapidly make data mining for the increasing images has become the most challenging problem faced by people. This paper focuses on data mining of the massive images with the help of the Hadoop cloud platform.
MemoMarote: a working memory training game

Mate Marote is a exible framework for large-scale educa- tional interventions that include games developed, based on aspects from cognitive sciences as an eective way of learning. MemoMarote is a com- puter game designed for training working memory. The pilot study of this platform was an intervention in La Rioja province (Argentina). In this article we analyze the registered statistics of use of the unsuper- vised version of the game. We found that playing activity had a peak when recently installed, decreasing during the rst year and observing an increase when starting school classes in 2013. Analyzing the usage statistics we observed that players have a poor performance, with more incorrect answers than correct ones. These results suggest that children did not read instructions or they were not understood, and so they played the game incorrectly. We plan the creation of an interactive tutorial to increase the understanding of the game.
Gel-based proteomics in plants: time to move on from the tradition

In more than 20 years of the proteomics era, considerable technical developments and scientific discoveries have contributed to the advancement of this field of research. Gel-based proteomics has been popular for studying the proteomic changes during growth and development of plants as well as for the analysis of responses to different biotic and abiotic stimuli. The most widely used gel-based technique is two dimensional (2D) gel electrophoresis, in which around 2000 protein spots can be clearly visualized and processed prior to identification by mass spectrometry. Although many techniques have been developed to improve the quality and number of spots in a 2D gel, these enhancements are still not good enough to study the entire cellular proteome. It is important to also consider low abundant proteins, many of which could play critical roles in particular biological processes. Considering that thousands of proteins are expressed at a given time and each protein can undergo one or more post-translational modifications—are we able to capture all this information and the entire proteome using gel-based proteomic techniques? Are gel-free alternatives capable of addressing these challenges? The answer is no for both approaches. So, how long are we going to run traditional 1D and 2D gels in our research? Though we have been successful in developing many advanced gel-free proteomic techniques to study the cellular proteome, we still lack advances that enable the examination of each and every protein expressed in a system as well as any post-translational modification (PTM) at a given time. Gel-free and gel-based techniques may complement each other, however there needs to be synergy or a “technical fusion” in which researchers can find a balance between quick and efficient methods for studying whole cell proteomes in plants.
The antiosteoporotic effects of Cheonggukjang containing vitamin k2 (menaquinone-7) in ovariectomized rats.

Abstract The effect of dietary vitamin K2 (menaquinone-7, MK-7) and cheonggukjang (CGJ) on the prevention of ovariectomy (OVX)-induced bone loss was studied in rats. Female Sprague-Dawley rats were divided into eight groups: sham-operated; OVX control; OVX treated with MK-7 at doses of 2, 4, and 8 μg/day; and OVX treated with CGJ at doses of 0.063, 0.125, and 0.250 g/day referenced to MK-7 levels at 2, 4, and 8 μg/day, respectively. After 8 weeks of treatment, the preventive effects of MK-7 and CGJ were evaluated by measuring body weights, serum levels of bone turnover markers, bone mineral content (BMC), bone mineral density (BMD), trabecular microarchitectural properties, and bone histological characteristics. Our results showed that rats treated with a high dose of MK-7 (8 μg/day) exhibited a minor inhibitory effect on OVX-induced bone loss, as indicated by a significant increase in trabecular number, as well as BMC and BMD (P<.01). Moreover, the preventive effects of MK-7 were augmented by administrat.
Expanding indications of minimally invasive esophagectomy in elderly patients

Esophagectomy has always been associated with a high risk of morbidities. Using the recent definitions reported by the Esophageal Complication Consensus Group (ECCG), the same group published results of >2,700 esophagectomy performed in 24 centers from a newly developed web-based database system (1,2). The overall complication rate was 59.0% and the 30-day mortality was 2.4%.
An Attempt to Create an Automatic Scoring Tool of Short Text Answer in Bahasa Indonesia

Closed questions offer poor information on student's ability to manage and apply knowledge. On the other hand, open questions have advantages because it may be used to grasp students' conceptual maturity and ability of communication. However, scoring open question answer is not trivial and time-consuming so an automatic scoring tool becomes necessary. An attempt was made to create a scoring tool for open and short text question answer in Bahasa Indonesia that resembles the way school teachers do scoring. Automatic scoring of a student answer was based on the similarity between the answer and predefined key answers. The proposed automatic scoring tool has a form of correlation with human scoring so that the model may be used to predict teacher scoring.
Bio-inspired Tetrakaidecahedron Tensegrity Optimisation

Through evolution, nature has solved many problems for living creatures in accordance with their needs and survival in direct response to physical forces. Among all needs of living creatures, mechanical stability and structural performance are of very high importance. Therefore, biomimicry can be a key to engineering mechanical problems. Cytoskeleton (CSK) is a network composed of filamentous biopolymers that mechanically stabilises the cell and actively generates prestresses resisted by external adhesive tethers to the extracellular matrix (ECM). The CSK and ECM form a synergetic tensegrity system. In addition, the three-dimensional tissue scaffolds is modelled as a periodic unit cell of tetrakaidecahedron geometry that packs to fill space. Optimisation is an unavoidable process for tensegrity analyses and design. In most cases of tensegrity optimisation research, the topology and connectivity are predefined. However, relaxing connectivity constraint has resulted in heavy and expensive formulation and computations in some limited researches. In this paper, imitating the tissue scaffold, the tetrakaidecahedral tensegrity is investigated. A convex optimisation is performed with relaxing the nodal predefined connectivity. The objective is to minimise the number of prestress modes by minimising the rank of weighted Laplacian matrix. The present method is concise and can be utilised for large-scale tensegrity systems.
An expert system to forecast visibility in Don-Muang Air Force Base

This article describes an expert system implemented to assist climatologists in forecasting visibility. The forecast made is for Don-Muang Air Base in Bangkok. The system is based on a verbal model derived from an experienced human forecaster. The inference mechanism used is of fuzzy type. The system is currently investigated to assess its reliability. <<ETX>>
Assessment of estrogen receptor, progesterone receptor of infiltrating ductal carcinoma of breast for neoadjuvant chemotherapy

Background: The aims and objectives of this study are to study the influence of Estrogen receptor ER, Progesterone receptor PR of patients having infiltrating ductal carcinoma of breast recieving Neoadjuvant chemotherapy, To study the influence of ER and PR receptors in patients receiving neoadjuvant chemotherapy & correlate complete pathological response. Methods  Type of study: Prospective clinical study. present study was carried out in the department of general surgery Vinayaka Missions medical college and hospital karaikal from July 2016 – December 2017.  HPE (Histopathological examination) and ER PR status was done. Patients diagnosed with locally advanced breast cancer underwent simple randomization into 2 treatment groups. Group 1: AC (Adriamycin 60mg/m2, Cyclophosphamide 600mg/m2) once every 3 weeks for 4 cycles Group2: Docetaxel 75 mg/m2 for 4 cycles every 3 weekly(11). Clinical examination was performed after every two cycles and completing all the chemotherapy, study group patients were subjected to Modified radical mastectomy which was then followed by evaluation of pathological response. Conclusion: In this study 30 patients ER PR status was done. According to ER PR status Neoadjuvant chemotherapy regimen given. NCT response was assessed with clinical / imaging study. There was reduction in tumor size and change in lymph node status in both the groups with p value of . 44. As our study size is small we cannot predict the actual outcome of the two study groups. We need larger sample size to predict the actual outcome. Results: The statistical analysis was performed by STATA 11.2. Descriptive statistics of frequency and percentage were performed all the study variables. Chi square test were used to measure the association between the CP response with ER status. P<0.05 considered as statistically significance.
Controlling overhead in large Python programs

Computational overhead is parts of a program’s run time not directly attributable to the job the program is intended to do. In the context of high-level dynamic languages, the term overhead is often used to describe performance in relation to C. In this thesis I use The Genomic Hyperbrowser, a large and complex Python program, as the case in a study of controlling computational overhead. Controlling computational overhead can have at least three different meanings, all of which are considered in this thesis. First, it can mean to assess the amount of overhead in a given program, i.e. estimate how much faster the program could run according to some notion of optimal execution. Several ways of defining overhead is covered in this thesis, together with ways to estimate overhead based on these definitions. Furthermore, I propose ways to manually inspect the run time distribution in programs to determine what parts of your code are slow. Second, it can mean to limit excess overhead in a program by using specialized tools and techniques to find parts of the code that are particularly good candidates for code optimization to reduce overall running time. Here, I show step by step a real case of finding, analyzing and resolving a performance issue caused by excessive overhead. A third meaning can be to design a program in such a way as to informally bound the overhead in the final program, i.e. to apply simple design principles that can help avoid the overhead ending up at an inappropriate level. In this thesis I show how central design decisions in the HyperBrowser ended up affecting computational overhead.
Expanding a Corpus of Closed-World Descriptions by Semantic Unit Selection

We present a method for the controlled expansion of a corpus based on “semantic unit selection”: units from a speech database are chosen not for closeness to an acoustic or phonetic target, but rather for their semantic content. While unsuited for general speech synthesis, it may be useful for restricted domains. We provide an application example from our current line of research: induction of lexical structure (i.e., acoustic, combinatorial, and semantic information) from unanalyzed recordings of informants describing small, closed-world scenarios. Here, semantic unit selection permits existing descriptions to be freely paraphrased and rearranged into new ones. The amount of redundancy can be parameterized, offering a way to control the difficulty to the task. The method is not dependent on the original scene described but can take a formal description of a new scene as input, or even enumerate all scenes describable by the data (along with descriptions).