Miracle-dz/UniFAR-FTUs huggingface dataset:
Profiling of mitochondrial associated proteins from rat colon

Mitochondrial dysfunction, damage and mutations of mitochondrial proteins give rise to a range of ill understood patterns of disease. Although there is significant general knowledge of the proteins and the functional processes of the mitochondria, there is little knowledge of difference about how mitochondria respond and how they are regulated in different organs and tissues. Proteomic profiling of mitochondria and associated proteins involved in mitochondrial regulation and trafficking within cells and tissues has the potential to provide insights into mitochondrial dysfunction associated with many human diseases. The rat colon mitoproteome analysis presented here provides a useful tool to assist in identification and interpretation of mitochondrial dysfunction implicated in colon pathogenesis. 2DPAGE followed by LC/MS/MS was used to identify 430 proteins from mitochondrial enriched fractions prepared from rat colon, resulting in 195 different proteins or approximately 50% of the resolved proteins being identified as multiple protein expression forms. Proteins associated with the colon mitoproteome were involved in calcium binding, cell cycle, energy metabolism and electron transport chain, protein folding, protein synthesis and degradation, redox regulation, structural proteins, signalling and transporter and channel proteins. The mitochondrial associated proteins identified in this study of colon tissue complement and are compared with other recently published mitoproteome analyses from other organ tissues, and will assist in revealing potentially organ specific roles of the mitochondria and organ specific disease associated with mitochondrial dysfunction. J. Cell. Biochem. 103: 78–97, 2008. © 2007 Wiley‐Liss, Inc.
Consonance theory part I: consonance of dyads.

Extensive psychological experiments were carried out in this Part I on the consonance sensation of various dyad tones consisting of two components. As the frequencies of two components f1 and f2 (with an equal SPL) separate, the consonance gradually decreases down to the most dissonant point, whereafter it monotonically increases and mostly recovers at an octave separation. This consonance characteristic turned out to show a simple V curve if consonance is plotted against the frequency percent deviation in a logarithmic scale, and it suggests a dynamic and a static factor in consonance perception. The most dissonant frequency deviation is approximately 10% at f1=440 Hz, and it increased with sound pressure and frequency, but it was not simply proportional to the critical bandwidth. For dyads whose two components have different sound‐pressure levels (SPLs) L1 and L2, the consonance differs with spectrum forms, even when the level difference is the same. A dyad with a spectrum form (P1>P2, f1
Regulation of Virus-triggered Signaling by OTUB1- and OTUB2-mediated Deubiquitination of TRAF3 and TRAF6*

Ubiquitination and deubiquitination have emerged as critical post-translational regulatory mechanisms for activation or attenuation of the virus-triggered type I interferon (IFN)2 induction pathways. In this study, we identified two deubiquitinating enzymes, OTUB1 and OTUB2, as negative regulators of virus-triggered type I IFN induction. Overexpression of OTUB1 and OTUB2 inhibited virus-induced activation of IRF3 and NF-κB, transcription of the IFNB1 gene as well as cellular antiviral response, whereas knockdown of OTUB1 and OTUB2 had opposite effects. Coimmunoprecipitations indicated OTUB1 and -2 interacted with TRAF3 and TRAF6, two E3 ubiquitin ligases required for virus-triggered IRF3 and NF-κB activation, respectively. Furthermore, we found that OTUB1 and OTUB2 mediated virus-triggered deubiquitination of TRAF3 and -6. These findings suggest that OTUB1 and OTUB2 negatively regulate virus-triggered type I IFN induction and cellular antiviral response by deubiquitinating TRAF3 and -6.
Efficient implementation of secondary synchronization symbol detection in 3GPP LTE

Cell search has to be performed by the user equipment in 3GPP Long Term Evolution (LTE) systems to obtain cell identity before connecting to the cell. The cell identity is transmitted using the Primary Synchronization Symbol (PSS) and the Secondary Synchronization Symbol (SSS). The PSS carries one of three possible values for the sector identity whereas the SSS carries one of 168 possible values for the physical cell identity. Straightforward implementation of SSS detection incurs a large area due to the large number of required correlators. This paper presents a complete design and hardware implementation for the detection of the SSS. The fixed point representation of the proposed system design is optimized to use the smallest word lengths at various system nodes while maintaining the overall performance within acceptable limits. Different architectures are studied, compared and an all-serial architecture is chosen to minimize the design area and power consumption, while achieving an acceptable acquisition time.
A combined sensor system of digital camera with LiDAR

In order to utilize the advantages of the high height accuracy of laser ranging and the good planimetric accuracy of processed digital camera imagery, the feasibility of a combined sensor system of LiDAR with digital camera using area or line array CCDs is first analyzed in this paper. The hardware composition of the combination system is given and the algorithm of integrally processing LiDAR points cloud and digital camera image is illustrated. Software development and availability for the processing at all stages of the work flow, is the key to the full utilization of such an integrated system.
Between‐ and within‐day variability in physical activity and inactivity in 9‐ and 15‐year‐old European children

To examine differences in levels of physical activity (PA), time spent at moderate‐to‐vigorous intensity PA (MVPA) and time spent sedentary between and within days in children from four European countries, 1954 9 – and 15‐year‐olds were included. PA was measured during 2 weekdays and 2 weekend days using the manufacturing technology‐incorporated (MTI) accelerometer. Average count per minute, time spent sedentary, time spent at MVPA and the proportion of children accumulating ≥60 min of MVPA were calculated. Data were compared between weekdays and weekend days and between school time and leisure‐time. Although not entirely consistent across countries, overall PA, time spent sedentary and the proportion of children accumulating ≥60 min of MVPA were higher during weekdays compared with weekend days. Differences in overall PA between school time and leisure‐time were highly inconsistent between countries. Few children (4–31%) accumulated ≥60 min of MVPA either during school time or during leisure‐time. Differences in activity patterns between weekdays and weekend days are explained by less accumulated time in MVPA during weekend days. Weekend days and leisure‐time during weekdays seem appropriate targets when promoting PA in order to increase the proportion of children achieving current recommendations on health‐enhancing PA.
A novel adaptive structure for SOA system effort estimation

This paper addresses a novel adaptive structure to solve the problem of service‐oriented architecture (SOA) system effort estimation. The objective of our work is to combine the insights of signals theory with empirical research to find a recommendation scheme for the problem of SOA system effort estimation. The main motivation for using this structure is to enhance the principle of self‐adaptability to the situation at hand. The proposed structure consists of an adaptive filter composed of linear combiner of filter weights, input and desired values. Additionally, a gradient steepest descent method is used to adjust the filter parameters (training process) using the least mean square algorithm. Furthermore, an experimental analysis is conducted with the proposed structure using the data of 10 past SOA system industrial applications, and in the empirical analysis, two performance measurement metrics and an evaluation function are used to assess the performance in terms of predictive accuracy. The experimental analysis and comparison study are helpful to demonstrate the effectiveness of an estimation technique. The obtained results indicate that an improved predictive accuracy for the problem of SOA system effort estimation has been achieved using the proposed structure when compared with support vector machines, linear, stepwise and ordinary least square regression techniques. Copyright © 2016 John Wiley & Sons, Ltd.
Lernmotivation und Selbstregulation

"Lernen soll Spas machen", das ist eine Devise, die in der heutigen Lehr-Lernforschung weit verbreitet ist und entsprechend verbreitet sind auch die Bemuhungen, den Lehrenden Moglichkeiten aufzuzeigen, wie sie dieses Ziel erreichen konnen. Es ist naturlich nichts dagegen einzuwenden, dass Lernen Spas macht, die Frage ist jedoch, ob es immer Spas machen kann. Alltagsbeobachtungen legen nahe, dass selbst eine hohe Lernmotivation kein Dauerzustand ist, sondern immer wieder durch Unlust-Phasen unterbrochen wird, die im Interesse der Erreichung eines langerfristigen Lern- oder Leistungsziels bewaltigt werden mussen. Doch wie und wodurch kann eine solche Bewaltigung geleistet werden? Die Auseinandersetzung mit dieser Frage soll das Hauptthema der folgenden Ausfuhrungen sein. Im ersten Teil geht es um die Frage, wie eine, die intrinsische Lernmotivation erganzende extrinsische Lernmotivation zu charakterisieren ist und was Lehrende tun konnen, um diese Form der extrinsischen Motivation zu fordern. Der zweiteTeil konzentriert sich auf die Darstellung von selbst regulatorischen Masnahmen, die um mit Huxley (1897) zu sprechen den Lernenden befahigen "to make yourself do the thing you have to do, when it ought to be done, whether you like it or not. " (zitiert nach Lepper 2000, S. 298).
Energy-efficient task offloading for multiuser mobile cloud computing

Mobile cloud computing enables the resource-poor mobile terminals to deal with the resource-hungry applications thanks to the extra storage and computational resources at the cloud side. However, the advantages of mobile cloud computing cannot be fully exploited without proper collaboration of radio transmission and computing, which is challenging under multiuser scenarios due to the coupling of radio and computational resources. In this paper, targeting at reducing the terminal energy consumption, we study the joint optimization of radio and computational resources for multiple users in mobile cloud computing and propose a heuristic strategy, based on the latency constraint and the application type of each MT, for resource allocation of low computational complexity. Numerical results show that our proposed task offloading strategy can significantly reduce the total energy at the mobile terminal side by 40% with 3 mobile terminals in the system, compared with the non-offloading mobile computing scheme, while at the same time satisfying the delay constraints. Moreover, it performs fairly close to the optimum.
Measurement of coal particle combustion behaviors in a drop tube furnace through high-speed imaging and image processing

This paper presents the measurement and characterization of single coal particles in a drop tube furnace through high speed imaging and image processing. A high speed camera coupling with a long distance microscope is employed to acquire the images of the particle during its residence time in the furnace. A set of physical quantities of the particle, including size, shape and boundary roughness, are defined and computed based on the images obtained, which are then used describe the combustion behaviors of the particle. Experimental results show that the combined high speed imaging and image processing technique has provided an effective means for measuring and quantifying the characteristics of single coal particles during combustion.
The Initiator Core Promoter Element Antagonizes Repression of TATA-directed Transcription by Negative Cofactor NC2*

Core promoter regions of protein-coding genes in metazoan genomes are structurally highly diverse and can contain several distinct core promoter elements, which direct accurate transcription initiation and determine basal promoter strength. Diversity in core promoter structure is an important aspect of transcription regulation in metazoans as it provides a basis for gene-selective function of activators and repressors. The basal activity of TATA box-containing promoters is dramatically enhanced by the initiator element (INR), which can function in concert with the TATA box in a synergistic manner. Here we report that a functional INR provides resistance to NC2 (Dr1/DRAP1), a general repressor of TATA promoters. INR-mediated resistance to NC2 is established during transcription initiation complex assembly and requires TBP-associated factors (TAFs) and TAF- and INR-dependent cofactor activity. Remarkably, the INR appears to stimulate TATA-dependent transcription similar to activators by strongly enhancing recruitment of TFIIA and TFIIB and, at the same time, by compromising NC2 binding.
Activated neu oncogene sequences in primary tumors of the peripheral nervous system induced in rats by transplacental exposure to ethylnitrosourea.

Neurogenic tumors were selectively induced in high incidence in F344 rats by a single transplacental exposure to the direct-acting alkylating agent N-ethyl-N-nitrosourea (EtNU). We prepared DNA for transfection of NIH 3T3 cells from primary glial tumors of the brain and from schwannomas of the cranial and spinal nerves that developed in the transplacentally exposed offspring between 20 and 40 weeks after birth. DNA preparations from 6 of 13 schwannomas, but not from normal liver, kidney, or intestine of tumor-bearing rats, transformed NIH 3T3 cells. NIH 3T3 clones transformed by schwannoma DNA contained rat repetitive DNA sequences, and all isolates contained rat neu oncogene sequences. One schwannoma yielded a transformant with rat-specific sequences for both neu and N-ras. A point mutation in the transmembrane region of the putative protein product of neu was identified in all six transformants and in the primary tumors from which they were derived as well as in 5 of 6 schwannomas tested that did not transform NIH 3T3 cells. Of 59 gliomas, only one yielded transforming DNA, and an activated N-ras oncogene was identified. The normal cellular neu sequence for the transmembrane region, but not the mutated sequence, was identified in DNA from all 11 gliomas surveyed by oligonucleotide hybridization. Activation of the neu oncogene, originally identified [Schechter, A.L. , Stern, D.F. , Vaidyanathan, L. , Decker, S.J., Drebin, J.A. , Greene, M.I. & Weinberg, R.A. (1984) Nature (London) 312, 513-516] in cultured cell lines derived from EtNU-induced neurogenic tumors that by biochemical but not histologic criteria were thought to originate in the central nervous system in BD-IX rats, appears specifically associated with tumors of the peripheral nervous system in the F344 inbred strain.
Low communication 2-prover zero-knowledge proofs for N P ( Preliminary Version )

We exhibit a two-prover perfect zero-knowledge proof system for 3-SAT. In this protocol, the verifier asks a single message t o each prover, whose size grows logarithmically in the size of the %SAT formula. Each prover's answer consists of only a constant number of bits. T h e verifier will always accept correct proofs. Given a n unsatisfiable formula S the verifier will reject with probability a t least O((lSI-max-sat(S))/lSl, where max-sat (S) denotes the maximum number of clauses of S that may be simultaneously satisfied, arid 1.5' denotes the total number of clauses of S. Using a recent result by Arora e t al [2], we can construct for any language in NP a protocol with the property tha t any non-member of the language be rejected with constant probability.
Study on topological deformation of point-sampled surfaces

A novel method integrates the topological flexibility of the level-set approach and the simplicity of point-sampled surfaces in this paper. The grid structure resulted from the level-set approach not only offers a wide range of powerful surface editing techniques for the point set surface editing, but also facilitates the topology change with ease. With the aid of point-based re-sampling, the method quickly updates the surface shape of the point-based geometry without worrying about point connectivity at all. The point set surface can also change its topology properly whenever a collision with other parts of itself is detected. The experiment demonstrates their effectiveness on several scanned objects and scan-converted models. Four examples of surface editing operation is presented: smoothing, tapering, deforming, Boolean operation.
Evx1 is required for joint formation in zebrafish fin dermoskeleton

The transcription factor Evx1 is expressed in the joints between individual lepidotrichia (bony ray) segments and at the distal tips of the lepidotrichia in developing zebrafish fins. It is also expressed in the apical growth zone in regenerating fins. However, so far there is no functional evidence that addresses whether Evx1 is required for any aspect of fin development or regeneration. In this study, we use a novel mutation in evx1 to address this. We find that Evx1 is not required for either fin outgrowth or regeneration. All of the fins form normally in evx1 mutants, and there are no significant changes in fin length. In contrast, Evx1 is required for lepidotrichia joint formation during both fin development and regeneration. This is a very specific phenotype as both lepidotrichia hemisegment separations and lepidotrichia bifurcations still form normally in evx1 mutant fins, as do joints in the more proximal endoskeletal radials. Developmental Dynamics 240:1240–1248, 2011. © 2011 Wiley‐Liss, Inc.
EFFECTS OF DIETARY PROTEIN LEVELS ON OOCYSTS SHEDDING IN BROILERS DURING SUBCLINICAL COCCIDIOSIS

We aimed to study the interaction between different dietary protein levels intake and the evaluation of response to a coccidial challenge by counting oocysts per gram (OPG) in broiler chicks. 70 chicks were divided into 7groups of ten chicks each. An uninfected control group (group 1) was fed a diet based on standard protein content (20%) and others (group 2 to 7) were fed diets based on dietary protein levels of 16%, 18%, 20%, 22%, 24% and 26% protein, respectively. At 15 d of age, treatments 2 to7 were subjected to a single dose infection of 50000 oocysts of Eimeria. OPG of all groups was counted at days 5 and 14 before inoculation and days 4, 5, 8 and 10 post infection. The results indicated the mean oocyst index in all days post infection in groups 2 and 3 was a little more than the fourth group with diet based on standard protein content, whereas groups 5, 6 and 7 with diet protein of 22%, 24% and 26% shed oocysts lesser than group four. In fact an increase in dietary protein led to a decrease (P<0.01) in oocysts shed by infected chicks. Also the highest OPG (worst) was belong to group 2 in day 10 after infection while the lowest OPG (better) was calculated for the seventh group in day 3 after inoculation. We realized protein increased intake causes considerable decrease in OPG and makes the changes rising in OPG slow in alternative sampling post infection.
Vancomycin Resistant Enterococci in a Tertiary Care Hospital in Mumbai

compares well with MGIT in terms of recovery rate and detection time, and detects mycobacterial growth much faster than the LJ medium. The Bio FM vial is about 30% cheaper than the MGIT vial and does not need any capital investment. Thus, it can be used in settings where cost and/ or infrastructural limitations prohibit usage of fully automated systems. Also, its usage will facilitate reporting of TB cultures earlier than the conventional LJ method at a more reasonable cost to the patient, thereby improving overall patient management. Nevertheless, larger studies are recommended to evaluate the sensitivity, speciÞ city and contamination rates of the medium, before putting to diagnostic use.
Isolation and Characterization of an Arabidopsis Mutant

Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060–8589, Japan (N.O.-O., S.N.); Department of Applied Biological Chemistry, Graduate School of Agricultural Life Sciences (I.K.), and Biotechnology Research Center (T.F.), The University of Tokyo, Tokyo 113–8657; and Precursory Research for Embryonic Science and Technology, Japan Science and Technology Corporation, Kawaguchi, Saitama 332–0012, Japan (T.F.)
The Nature and Impact of Gendered Patterns of Peer Sexual Communications Among Heterosexual Emerging Adults

Although previous research demonstrates that peers serve as top sexual informants and advisers, little is known about how peer sexual communications may be a gendered phenomenon. Do communications about sex and romantic relationships vary according to who is speaking to whom? The current study examined 517 college students’ reports of male and female peers’ communications of four sexual scripts and the associations between reports of such communications and participants’ sexual attitudes and levels of sexual and dating experience. Results suggest that peer messages about sex and relationships vary by the gender of the recipient and the gender of the communicator. Women reported more frequent communications of all sexual scripts from female peers than did men. In terms of male peers’ sexual communications, only one gender difference emerged: men reported receiving significantly fewer messages about the relational script than women. Compared to same-sex peer communications, there were more associations between other-sex peer communications and undergraduates’ sexual attitudes and levels of sexual and dating experience. Implications for the role of same- and other-sex peers in sexual socialization are discussed.
Lethal outcome of 5-fluorouracil infusion in a patient with a total DPD deficiency and a double DPYD and UTG1A1 gene mutation.

5-Fluorouracil (5-FU)-based chemotherapy has been widely used for almost 50 years in the treatment of solid malignancies, especially in digestive cancers. Its main effect consists in the inhibition, via its active metabolite, of thymidylate synthase, a major enzyme involved in DNA synthesis. In normal conditions, less than 20% of the administered 5-FU is used in this anabolic pathway while more than 80% is catabolized via dihydropyrimidine dehydrogenase (DPD) [1]. This is the initial and rate-limiting enzyme in the catabolic pathway and therefore a partial or complete DPD deficiency is associated with different degrees of 5-FU toxicity whose most frequent manifestations include neutropenia, mucositis and diarrhoea [2].
[Amplification of oncogenes in solid tumors in children].

BACKGROUND The objective of the work was detection of amplification of oncogenes N-MYC, N-RAS, C-ERB A, C-ERB B and adaptor tyrosine kinase Shb in a group of 92 child age tumours in an attempt to reveal clinical and histopathological associations. METHODS AND RESULTS Amplifications of oncogenes were detected by means of Southern's transfer, hybridization with labelled probes and densitometric evaluation. Amplification of the N-MYC oncogene in child tumours can be considered a manifestation of progression of the disease with an adverse prognosis, in particular in neuroblastomas, where it corresponds also with the adverse histological finding. In a group of sarcomas N-MYC amplification was detected in advanced clinical stages, while in malignant lymphogranulomas of the Hodgkin type it was not found. In Wilms tumour it was detected sporadically. Amplifications of oncogenes ERB A and ERB B are rare, amplifications of the oncogene RAS were not observed. Coamplifications characterized progression of the disease, in case of neuroblastoma even very short survival. In hepatic malignancies oncogene amplification was not found even in advanced stages. CONCLUSIONS Oncogene amplification characterizes progression in a number of child tumours and its application in clinical oncology is prognostically useful.