Miracle-dz/UniFAR-FTUs huggingface dataset:
Complete Revascularization with Multivessel PCI for Myocardial Infarction.

BACKGROUND In patients with ST-segment elevation myocardial infarction (STEMI), percutaneous coronary intervention (PCI) of the culprit lesion reduces the risk of cardiovascular death or myocardial infarction. Whether PCI of nonculprit lesions further reduces the risk of such events is unclear. METHODS We randomly assigned patients with STEMI and multivessel coronary artery disease who had undergone successful culprit-lesion PCI to a strategy of either complete revascularization with PCI of angiographically significant nonculprit lesions or no further revascularization. Randomization was stratified according to the intended timing of nonculprit-lesion PCI (either during or after the index hospitalization). The first coprimary outcome was the composite of cardiovascular death or myocardial infarction; the second coprimary outcome was the composite of cardiovascular death, myocardial infarction, or ischemia-driven revascularization. RESULTS At a median follow-up of 3 years, the first coprimary outcome had occurred in 158 of the 2016 patients (7.8%) in the complete-revascularization group as compared with 213 of the 2025 patients (10.5%) in the culprit-lesion-only PCI group (hazard ratio, 0.74; 95% confidence interval [CI], 0.60 to 0.91; P = 0.004). The second coprimary outcome had occurred in 179 patients (8.9%) in the complete-revascularization group as compared with 339 patients (16.7%) in the culprit-lesion-only PCI group (hazard ratio, 0.51; 95% CI, 0.43 to 0.61; P<0.001). For both coprimary outcomes, the benefit of complete revascularization was consistently observed regardless of the intended timing of nonculprit-lesion PCI (P = 0.62 and P = 0.27 for interaction for the first and second coprimary outcomes, respectively). CONCLUSIONS Among patients with STEMI and multivessel coronary artery disease, complete revascularization was superior to culprit-lesion-only PCI in reducing the risk of cardiovascular death or myocardial infarction, as well as the risk of cardiovascular death, myocardial infarction, or ischemia-driven revascularization. (Funded by the Canadian Institutes of Health Research and others; COMPLETE ClinicalTrials.gov number, NCT01740479.).
[The long coding RNA GSTM3TV2 acts an oncogene to promote chemoresistance in pancreatic cancer].

Objectives: To examine the expression of the long coding RNA GSTM3TV2 in pancreatic cancer tissues and to examine its role and mechanism in chemoresistance of pancreatic cancer cells. Methods: The expression of lncRNA GSTM3TV2 in 15 pancreatic cancer specimens and corresponding adjacent to cancer tissue samples diagnosed by Department of Pathology, Peking Union Medical College Hospital was detected by real-time PCR.And the expressions of GSTM3TV2 in pancreatic cancer cell AsPC-1, BxPC-3, MIAPaCa-2, PanC-1, SU86.86, T3M4, and chemoresistant cells AsPC-1/GR and MIAPaCa-2/GR, and human pancreatic nestin-expressing cells hTERT-HPNE were detected. Pancreatic cancer cell lines were transfected with GSTM3TV2-pcDNA3.1(+)in order to get cells with GSTM3TV2 overexpression.GSTM3TV2-siRNA was transfected into pancreatic cancer cells to knock down GSTM3TV2. The cell chemoresistance was measured by CCK-8 and flow cytometry assay when incubated with nab-paclitaxel. At the same time, subcutaneous xenograft tumor models were established in nude mice to observe the effect of GSTM3TV2 on chemoresistance of tumor growth in nude mice.Western blot assay was also performed to detect the molecular mechanism of chemoresistance of GSTM3TV2. Results: Comparing toadjacent tissues(0.084±0.019), GSTM3TV2 expression was significantly upregulated in the pancreatic cancer tissues(0.493±0.084) (t=5.146, P<0.05). GSTM3TV2 expression were higher in the chemotherapy resistance pancreatic cancer cells AsPC-1/GR(210.799±19.788) and MIAPaCa-2/GR(122.408±23.419) than that in the AsPC-1(3.793±0.615) and the MIAPaCa-2(5.179±1.095)(t=21.800,P<0.05;t=-18.490,P<0.05). The results of in vivo experiments showed that the volume of subcutaneously transplanted tumors in the overexpressing GSTM3TV2 group ((1 059.609±102.498)mm(3)) was significantly larger than that in the control group((566.414±81.087) mm(3)) by treated with nab-paclitaxel(t=4.230,P<0.05). Meanwhile, GSTM3TV2 could promote the expression of Cyclin D1, CDK6, Cyclin E1, Vimentin, N-cadherin, ZEB1, Snail and Slug; but decrease cleaved caspase-3, cleaved PARP in pancreatic cancer cells. Conclusions: The expression level of GSTM3TV2 in pancreatic canceris higher than that in paired adjacent tissues. GSTM3TV2 may act as an oncogene to promote chemoresistance in pancreatic cancer through regulation of cell proliferation, apoptosis, and epithelial-mesenchymal transition.
United Republic of Tanzania GENERAL INFORMATION OBJECTIVE I. REDUCE DIRECT AND INDIRECT CAUSES OF MARINE TURTLE MORTALITY

Tanzania’s mainland coastline, together with Zanzibar and numerous smaller offshore islands, provides important feeding and breeding habitats for five of the world’s seven marine turtle species: leatherback (Dermochelys coriacea), green (Chelonia mydas), hawksbill (Eretmochelys imbricata), loggerhead (Caretta caretta) and olive ridley (Lepidochelys olivacea) turtles. Green turtles are the most common species and are reported to nest throughout most of the coastline. Hawksbills are also widely distributed in Tanzania but are only known to nest in small numbers on off-shore islands. Very little information is available on olive ridley, loggerhead and leatherback turtles although they are thought to forage in Tanzanian waters and pass through en route to nesting sites elsewhere in the region.
Doctor and patient awareness of treatment options for cervical intraepithelial neoplasia 1 (CIN 1): a survey questionnaire approach

Abstract The purpose of this study was to investigate doctors’ and patients’ perceptions of cervical intraepithelial neoplasia 1 (CIN 1) and its treatment methods. A survey questionnaire was offered to obstetrics and gynaecology doctors and patients with CIN 1 in 2017. Only 43% of patients knew of this disease. Regarding perceptions of its aetiology, 64% of the patients perceived human papillomavirus infection to be the main cause of CIN 1. Patients’ most preferred treatments were medication (20%), followed by alternative treatment (14%). Among doctors, regular follow-up was the most preferred method for managing CIN 1. The survey showed that current treatment modalities for CIN 1 were satisfactory to only half of doctors (50%) and patients (53%). Overall, 70% of doctors responded that new drug development for CIN 1 is needed. Although, CIN 1 is a low-grade lesion, doctors and patients expressed the desire for new therapeutic agents to manage it. IMPACT STATEMENT What is already known on this subject? In general, treatment is not recommended for CIN 1 because lesions are considered indicative of transient HPV infection and spontaneously regress in most patients. What do the results of this study add? Regular follow-up for CIN 1 were satisfactory to only half of doctors and patients. Thirty-six percent of patients wanted active treatment instead of regular follow-up. In addition, 70% of doctors responded that new drug development for CIN 1 is needed. What are the implications of these findings for clinical practice and/or further research? Our results support the need for therapeutic agents for CIN 1.
Compactness of Protein Folds Alters Disulfide‐Bond Reducibility by Three Orders of Magnitude: A Comprehensive Kinetic Case Study on the Reduction of Differently Sized Tryptophan Cage Model Proteins

A new approach to monitor disulfide‐bond reduction in the vicinity of aromatic cluster(s) has been derived by using the near‐UV range (λ=266–293 nm) of electronic circular dichroism (ECD) spectra. By combining the results from NMR and ECD spectroscopy, the 3D fold characteristics and associated reduction rate constants (k) of E19_SS, which is a highly thermostable, disulfide‐bond reinforced 39‐amino acid long exenatide mimetic, and its N‐terminally truncated derivatives have been determined under different experimental conditions. Single disulfide bond reduction of the E19_SS model (with an 18‐fold excess of tris(2‐carboxyethyl)phosphine, pH 7, 37 °C) takes hours, which is 20–30 times longer than that expected, and thus, would not reach completion by applying commonly used reduction protocols. It is found that structural, steric, and electrostatic factors influence the reduction rate, resulting in orders of magnitude differences in reduction half‐lives (900>t1/2>1 min) even for structurally similar, well‐folded derivatives of a small model protein.
Association of Change in N-Terminal Pro-B-Type Natriuretic Peptide Following Initiation of Sacubitril-Valsartan Treatment With Cardiac Structure and Function in Patients With Heart Failure With Reduced Ejection Fraction.

Importance In patients with heart failure and reduced ejection fraction (HFrEF), treatment with sacubitril-valsartan reduces N-terminal pro-b-type natriuretic peptide (NT-proBNP) concentrations. The effect of sacubitril-valsartan on cardiac remodeling is uncertain. Objective To determine whether NT-proBNP changes in patients with HFrEF treated with sacubitril-valsartan correlate with changes in measures of cardiac volume and function. Design, Setting, and Participants Prospective, 12-month, single-group, open-label study of patients with HFrEF enrolled in 78 outpatient sites in the United States. Sacubitril-valsartan was initiated and the dose adjusted. Enrollment commenced on October 25, 2016, and follow-up was completed on October 22, 2018. Exposures NT-proBNP concentrations among patients treated with sacubitril-valsartan. Main Outcomes and Measures The primary outcome was the correlation between changes in log2-NT-proBNP concentrations and left ventricular (LV) EF, LV end-diastolic volume index (LVEDVI), LV end-systolic volume index (LVESVI), left atrial volume index (LAVI), and ratio of early transmitral Doppler velocity/early diastolic annular velocity (E/e') at 12 months. Results Among 794 patients (mean age, 65.1 years; 226 women [28.5%]; mean LVEF = 28.2%), 654 (82.4%) completed the study. The median NT-proBNP concentration at baseline was 816 pg/mL (interquartile range [IQR], 332-1822) and 455 pg/mL (IQR, 153-1090) at 12 months (difference, P < .001). At 12 months, the change in log2-NT-proBNP concentration was correlated with changes in LVEF (r = -0.381 [IQR, -0.448 to -0.310]; P < .001), LVEDVI (r = 0.320 [IQR, 0.246 to 0.391]; P < .001), LVESVI (r = 0.405 [IQR, 0.335 to 0.470]; P < .001), LAVI (r = 0.263 [IQR, 0.186 to 0.338]; P < .001), and E/e' (r = 0.269 [IQR, 0.182 to 0.353]; P < .001). At 12 months, LVEF increased from 28.2% to 37.8% (difference, 9.4% [95% CI, 8.8% to 9.9%]; P < .001), while LVEDVI decreased from 86.93 to 74.15 mL/m2 (difference, -12.25 mL/m2 [IQR, -12.92 to -11.58]; P < .001) and LVESVI decreased from 61.68 to 45.46 mL/m2 (difference, -15.29 mL/m2 [95% CI, -16.03 to -14.55]; P < .001). LAVI and E/e' ratio also decreased significantly. The most frequent adverse events were hypotension (17.6%), dizziness (16.8%), hyperkalemia (13.2%), and worsening kidney function (12.3%). Conclusions and Relevance In this exploratory study of patients with HFrEF treated with sacubitril-valsartan, reduction in NT-proBNP concentration was weakly yet significantly correlated with improvements in markers of cardiac volume and function at 12 months. The observed reverse cardiac remodeling may provide a mechanistic explanation for the effects of sacubitril-valsartan in patients with HFrEF. Trial Registration ClinicalTrials.gov Identifier: NCT02887183.
Dengue virus serotype-4 during the 2015-2017 epidemic: emergence of a novel genotype IIa of DENV-4 in the Philippines.

Dengue remains a major public health problem in the Philippines. Here, we determined the circulating serotypes in the Philippines during the 2015-2017 outbreaks by using a total of 678 serum samples that were collected from 537 individual dengue patients. Following an increase in the percentage of DENV-4 patients in recent years, we conducted a comprehensive molecular epidemiology analysis on the DENV-4 strains isolated recently in the Philippines. While two genotypes of DENV-4 have been isolated in the Philippines since 1956, the GI and GIIa which were the DENV-4 strains isolated in this study were closely related to a distinct group of GIIa strains that were isolated from the Philippines since 2004. A majority of the isolates of this sub-group has been identified in the Philippines, suggesting that the lineage may have been introduced and evolved in the Philippines to form the distinct sub-group within GIIa strains. The increase in DENV-4 activity also coincided with the appearance of GIIa sub-group and the phasing-out of GI lineage in the Philippines. Overall, our study demonstrates a shift in DENV-4 genotype and epidemic dynamics in a hyperendemic region, suggesting the importance of DENV genetic evolution in establishing and sustaining transmission.
An Integrated Kidney Care eConsult Practice Model: Results from the iKinect Project

Background: Collaborative management of kidney disease relies on coordinated and effective partnerships between multiple provider teams. Siloed care contributes to limited access between physicians, resulting in delays in the diagnosis and treatment of kidney disease and inappropriate use of healthcare resources. These gaps contribute to dissatisfied and disempowered providers and patients. Digital systems such as eConsult can support collaborative management and address these gaps, thereby streamlining the consultation and referral process between primary care physicians (PCPs) and nephrologists. In this study, we evaluated an established eConsult platform integrated with a central triage process for a network of PCPs and nephrologists. The study aimed to assess the acceptability, feasibility, and impact on access to nephrology when using eConsult integrated into the management of kidney disease between PCPs and nephrologists. Methods: We conducted a 1-year pilot study and used mixed methods to measure the acceptability and feasibility of using eConsult for the management of kidney disease. We compared eConsult and traditional referrals with respect to types of consultation, referrals, and times to response to determine impact on access to kidney care. We conducted semi-structured interviews of PCPs and nephrologists to assess physician experience. Results: From January 8, 2018, to January 11, 2019, 52 PCPs and 23 nephrologists participated in the study, with 250 traditional referrals and 106 eConsults submitted during that period. The median response time for eConsult was 15 (3–64) h, with 25% originating outside the central Toronto region. The median time to first clinic appointment from a traditional referral was 4 months (111 [61–163] days). PCP and nephrologist interviews revealed high user satisfaction, citing efficiency and timely response as key facilitators. Conclusion: The eConsult platform was acceptable, feasible, and facilitated access to nephrology care compared to traditional referrals. Physicians report improvements in physician care delivery, nephrology care gaps, patient experience, and healthcare utilization.
COMPUTING DISTRIBUTED REPRESENTATIONS OF WORDS USING THE COROLA CORPUS

We investigate the usability of the CoRoLa corpus for generating high quality vector representations of words for Romanian language. Different model parameters are tested and model quality is compared in three test cases: two word analogies data sets and a word similarity correlation with human judgment. Furthermore, we prove that CoRoLa provides superior word representations compared to other known Romanian corpora, such as the Wikipedia corpus.
The AMP-activated Protein Kinase Snf 1 Regulates Transcription Factor Binding , RNA Polymerase II Activity , and mRNA Stability of Glucose-repressed Genes in Saccharomyces cerevisiae *

Elton T. Young, Chao Zhang, Kevan M. Shokat, Pabitra K. Parua, and Katherine A. Braun From the Department of Biochemistry, University of Washington, Seattle, Washington 98195-7350, the Department of Chemistry, Universit y of Southern California, Los Angeles, California 90089-0744, and the Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California 94143-2280
Effect of Biocides on Bacillus sp . Biofilms

The present study was to evaluate the bactericidal effects of biocides on Bacillus sp. biofilm formed on stainless steel. Tests were performed using different concentration of biocides, biofilms of 6 day old and a range of exposure times (from 0 (control) to 1, 5 and 20 minutes). The results provide clear evidences of resistance to detachment of Bacillus subtilis and Bacillus cereus attached to stainless steel, and for all the situations studied, bacterial biofilms were not completely removed (being still active) after biocide treatment.
Ignorance = doing what is reasonable: Children expect ignorant agents to act based on prior knowledge

When deciding how to act in new situations, we expect agents to draw on relevant prior experiences. This expectation underlies many of our mental-state inferences, allowing us to infer agents’ prior knowledge from their current actions. Do children share this expectation, and use it to infer others’ epistemic states? In Experiment 1, we find that fiveand six-year-olds (but not fouryear-olds) attribute additional knowledge to agents whose prior experiences cannot explain their success. In Experiment 2, we find that six-year-olds (but not younger children) also attribute greater knowledge to agents whose prior experience cannot explain their failure. We show that by age five or six, children expect ignorant agents’ beliefs (and therefore their actions) to be guided by their prior knowledge. This work adds to a growing body of research suggesting that, while infants can represent mental states, the ability to infer mental states continues to develop throughout early childhood.
Angiotensin-Neprilysin Inhibition in Heart Failure with Preserved Ejection Fraction.

BACKGROUND The angiotensin receptor-neprilysin inhibitor sacubitril-valsartan led to a reduced risk of hospitalization for heart failure or death from cardiovascular causes among patients with heart failure and reduced ejection fraction. The effect of angiotensin receptor-neprilysin inhibition in patients with heart failure with preserved ejection fraction is unclear. METHODS We randomly assigned 4822 patients with New York Heart Association (NYHA) class II to IV heart failure, ejection fraction of 45% or higher, elevated level of natriuretic peptides, and structural heart disease to receive sacubitril-valsartan (target dose, 97 mg of sacubitril with 103 mg of valsartan twice daily) or valsartan (target dose, 160 mg twice daily). The primary outcome was a composite of total hospitalizations for heart failure and death from cardiovascular causes. Primary outcome components, secondary outcomes (including NYHA class change, worsening renal function, and change in Kansas City Cardiomyopathy Questionnaire [KCCQ] clinical summary score [scale, 0 to 100, with higher scores indicating fewer symptoms and physical limitations]), and safety were also assessed. RESULTS There were 894 primary events in 526 patients in the sacubitril-valsartan group and 1009 primary events in 557 patients in the valsartan group (rate ratio, 0.87; 95% confidence interval [CI], 0.75 to 1.01; P = 0.06). The incidence of death from cardiovascular causes was 8.5% in the sacubitril-valsartan group and 8.9% in the valsartan group (hazard ratio, 0.95; 95% CI, 0.79 to 1.16); there were 690 and 797 total hospitalizations for heart failure, respectively (rate ratio, 0.85; 95% CI, 0.72 to 1.00). NYHA class improved in 15.0% of the patients in the sacubitril-valsartan group and in 12.6% of those in the valsartan group (odds ratio, 1.45; 95% CI, 1.13 to 1.86); renal function worsened in 1.4% and 2.7%, respectively (hazard ratio, 0.50; 95% CI, 0.33 to 0.77). The mean change in the KCCQ clinical summary score at 8 months was 1.0 point (95% CI, 0.0 to 2.1) higher in the sacubitril-valsartan group. Patients in the sacubitril-valsartan group had a higher incidence of hypotension and angioedema and a lower incidence of hyperkalemia. Among 12 prespecified subgroups, there was suggestion of heterogeneity with possible benefit with sacubitril-valsartan in patients with lower ejection fraction and in women. CONCLUSIONS Sacubitril-valsartan did not result in a significantly lower rate of total hospitalizations for heart failure and death from cardiovascular causes among patients with heart failure and an ejection fraction of 45% or higher. (Funded by Novartis; PARAGON-HF ClinicalTrials.gov number, NCT01920711.).
Optimal Climate Change Adaptation and Mitigation Expenditures in Environmentally Small Economies

We study the optimal role of mitigation and adaptation strategies for environmentally small economies, that is, economies that are witnessing an exogenous increase in emissions to which they are contributing very little. Our results lead to three main conclusions. First, small economies should concentrate their environmental efforts, if any, on adaptation. This is a recommendation based on cost effectiveness rather than on any idea about these economies indulging in free riding. Second, environmentally small economies that are unable to spend enough on adaptation may end up spending less on mitigation in the long term, owing to their impoverishment as a result of negative climate shocks. Third, higher mitigation expenditures may arise not only as a result of greater optimal adaptation expenditures, but also because of increased adaptation to the incentives for mitigation provided by richer countries. For the simulations, we use a calibrated optimal growth model for Brazil, Chile, and the United States.
SPLIT LAKE BLOCK OF THE SUPERIOR BOUNDARY ZONE: PETROLOGICAL, PALEOMAGNETIC AND GEOBAROMETRIC DATA FOR THE CA. 1818 MA FOX LAKE PLUTON (NTS 64A/1, /8)1

The ca. 1818 Ma Fox Lake pluton of the Split Lake Block on the northwestern margin of the Superior craton is one of several rock units collected and analyzed paleomagnetically to describe the tectonic motions for the craton during the waning stages of the Trans-Hudson Orogen. The results suggest that this pluton was emplaced ca. 1850 Ma and later deformed ca. 1810 Ma. Detailed petrological observations and preliminary pressure-temperature data are also presented for the pluton and host rocks. INTRODUCTION
Utricularia vulgaris L. and Salvinia natans (L.) All. heavy metal (Fe, Mn, Cu, Zn and Pb) bioaccumulation specificity in the area of Bardača fishpond

Abstract Paper deals with seasonal changes in heavy metal bioaccumulation (Fe, Mn, Cu, Zn and Pb) in Utricularia vulgaris L. and Salvinia natans (L.) All. of two localities (Necik and Sinjak – active fishpond basins) in the area of Bardača fishpond. According to our results, the better accumulator of Fe (3035 mg/kg) and Zn was S. natans (163.55 mg/kg), whereas Utricularia vulgaris better accumulates Mn (620 mg/kg) and Cu (10.18 mg/kg). Amounts of Pb in both investigated macrophyte were below the detection level (<0.51 mg/kg). The values of the bioaccumulation factor (BAC) of the tested metals were >1 or ~1 for both species, and the BAC values decreased in the following order in both investigated species: Fe > Mn > Cu > Zn. The results obtained indicated that tested macrophyte show very good characteristics as bioaccumulators and, thanks to this fact, they could be used in phytoremediation technique successfully in water-polluted ecosystem.