Miracle-dz/UniFAR-FTUs huggingface dataset:
Cyclin A2/Cdk1 is Essential for the in vivo S Phase Entry by Phosphorylating Top2a

Cyclin-dependent kinase 1 (CDK1) plays essential roles in cell cycle regulation. However, due to the early embryonic lethality of mouse Cdk1 mutants, the in vivo role of CDK1 in regulating cell cycle and embryonic development remains unclear. Here, by generating zebrafish cdk1 mutants using CRISPR/Cas9 system, we show that cdk1−/− embryos exhibit severe microphthalmia accompanied with multiple defects in polarized cell division, S phase entry and M phase progression, cell apoptosis and cell differentiation, but not in interkinetic nuclear migration (IKNM). By informatics analysis, we identified Top2a as a potential downstream target, and Cyclin A2 and Cyclin B1 as partners of Cdk1 in cell cycle. Depletion of either Cyclin A2 or Top2a leads to decreased S phase entry and increased DNA damage response in zebrafish retinal cells, and depletion of Cyclin B1 leads to M phase arrest. Immunoprecipitation shows that Cdk1 and Cyclin A2 physically interact in vivo. Moreover, phosphorylation of Top2a on Serine 1213 (S1213) site is almost absent in either cdk1 or ccna2 mutants, but in not ccnb1 mutants. Furthermore, overexpression of TOP2AS1213, the phosphomimetic form of human TOP2A, rescues S phase entry and microphthalmia defects in cdk1−/− and ccna2−/− embryos. Taken together, our data suggests that Cdk1 interacts with Cyclin A2 to regulate S phase entry through phosphorylating Top2a, and with Cyclin B1 to regulate M phase progression in vivo.
Use of psychoactive substances by young people reporting suicidal thoughts, plans, and attempts in the context of sociodemographic factors

Aim of the study: To analyse the prevalence of psychoactive substance use by young people reporting suicidal thoughts, plans, and attempts, including sociodemographic factors. Material and methods: The study involved 5685 individuals aged 13 to 19 years. Seventy percent ( n = 3980) of them were girls and 30% ( n = 1705) were boys. They attended schools in the Lublin Province in Eastern Poland. Forty-six percent ( n = 2615) of those surveyed were city dwellers and 54% ( n = 3070) lived in rural areas. The mean age of the participants was 16.91 years and the median was 17 years. The participants were surveyed using a self-report questionnaire designed by the study authors. Results: Statistically significant relationships were found between the participants’ suicidal behaviours (thoughts, plans, and attempts) and their use of alcohol and drugs. Conclusions: Individuals who report suicidal thoughts, plans, and attempts are more likely to abuse alcohol and use drugs than those who do not report suicidal behaviours. Significantly more young people living in the city than in the countryside abuse alcohol and take drugs. Women who live in urban areas start drinking at a significantly lower age than their peers living in the countryside.
Listeria monocytogenes soylarının genetik ve virülens farklılıkları

Listeria monocytogenes insanlarda ve hayvanlarda septisemi, menenjit, meningoensefalit, dusuk gibi ciddi invazif hastaliklara neden olabilen gida kaynakli bir patojendir. Epidemiyolojik arastirmalarda, gida isletmelerinden kontaminasyon kaynaginin takibinde ve farkli turler arasindaki iliskinin evriminin belirlenmesinde L. monocytogenes turlerinin alt tiplendirmesi cok onemlidir. Bu derlemede L. monocytogenes ’in soylari ile soylari arasindaki genetik ve virulens farkliliklarindan bahsedilmistir.
Neuronal morphology alterations in autism and possible role of oxytocin

Abstract Current understanding of the neuroanatomical abnormalities in autism includes gross anatomical changes in several brain areas and microstructural alterations in neuronal cells as well. There are many controversies in the interpretation of the imaging data, evaluation of volume and size of particular brain areas, and their functional translation into a broad autism phenotype. Critical questions of neuronal pathology in autism include the concept of the reversible plasticity of morphological changes, volume alterations of brain areas, and both short- and long-term consequences of adverse events present during the brain development. At the cellular level, remodeling of the actin cytoskeleton is considered as one of the critical factors associated with the autism spectrum disorders. Alterations in the composition of the neuronal cytoskeleton, in particular abnormalities in the polymerization of actin filaments and their associated proteins underlie the functional consequences in behavior resulting in symptoms and clinical correlates of autism spectrum disorder. In the present review, a special attention is devoted to the role of oxytocin in experimental models of neurodevelopmental disorders manifesting alterations in neuronal morphology.
USING SHIN LENGTH TO DETERMINE KICK PLATE POSITION OPTIMIZES SELECT SWIM START MECHANICS IN ELITE SWIMMERS

The track start kick plate position is often decided by the level of comfort of the swimmer. The purpose of this study was to use shin length as a measure to determine kick plate position and effects on performance. 20 elite swimmers performed 3 starts at 3 kick plate distances (< shin length, shin length, and > shin length). Differences in reaction time, block phase time (BT), flight phase time, flight distance, underwater phase time, time to the 15 m mark, knee flexion and ankle dorsiflexion angles were examined between the positions. BT was significantly different, (F(2,38)=4.264, p=. 026). BT was lower when the kick plate distance was one shin’s length versus < shin length (0.691+0.055 vs 0.715+0.056 sec) and > shin length (0.691+0.055 vs 0.698+0.056 sec), p<. 05. Shin length is a quick and individualized measure that can be used by coaches to set the kick plate position without compromising performance.
Hope and responsibility vested in younger generations: the case of Hungary

In the era of consumer society, the depletion of the world's natural resources brings conscious consumption in the spotlight. More and more people are said to be conscious consumers, who act responsibly with regard to the environment and society. Therefore, researches about conscious and responsible purchase behaviour draw the attention of economists and marketing specialists as well - thus being able to form their market actions. According to the stereotype about conscious consumers in our heads, the conscious consumer is young, well educated, and female. This paper aims to reveal the consumption habits of people and their attitude toward consciousness and responsibility, furthermore, to investigate them from the stereotype's point of view that finds youngsters more conscious. An online survey was conducted with 652 consumers of a German supermarket chain in Hungary. The research concentrates on identifying the main differences between generations regarding their purchase habits. The research results show that the consciousness is rather manifested in the self-interest than in the responsibility. While other international survey data have proven development through generations and found younger consumers more conscious and responsible, this fact has not been statistically proven in Hungary. The real causes or drivers behind conscious behaviour are still questionable in the Hungarian market.
G Protein Coupled Receptors-Mediated Chemotaxis in theModel Organism Dictyostelium Discoideum and Neutrophils

Chemotaxis, a directional cell migration guided by extracellular chemoattractant gradients, plays critical roles in many physiological processes, recruitment of neutrophils to sites of inflammation, metastasis of cancer cells, and development of model organism Dictyostelium discoideum [1-4]. Both D. discoideum and mammalian neutrophils sense chemoattractants using G protein-coupled receptors (GPCRs) and share remarkable similarities in the signaling pathways by which regulate cell migration Jin et al. [5]. It has been proven that D. discoideum is a powerful model organism to establish new concepts and identify new components essential for chemotaxis [6,7]. We developed and applied state-of-the-art live cell/single molecule imaging technologies to visualize spatiotemporal dynamics of GPCR-mediated signaling network in D. discoideum [8,9]. We also built up chemosensing signaling network for computational modeling [10]. By interplaying computational simulation and experimental verification (Figure1), we revealed locally-controlled inhibitory mechanism upstream of PI3K that is essential for chemosensing [9,11,12]. Recently, we are focusing on identifying the inhibitors, such as negative regulators of Ras, for chemosensing in both D. discoideum and mammalian neutrophils. Our long-term goal is to identify novel components and signaling pathways essential for chemotaxis to provide new therapeutic targets and strategies for inflammatory diseases and metastasis of cancer.
Iron Transport Machinery: A Potential Therapeutic Target In Escherichia Coli

Escherichia coli is a well-studied bacterium that can be found in many niches, such as industrial wastewater, where the concentration of nickel can rise to low millimolar levels. Recent studies show that nickel exposure can repress pyochelin or induce pyoverdine siderophore production in Pseudomonas aeruginosa (Braud et al., 2010). Understanding the molecular crosstalk between siderophore production, metal homeostasis, and metal toxicity in microorganisms is critical for designing bioremediation strategies for metal-contaminated sites. Here we show that high nickel exposure prolongs lag phase duration as a result of low intracellular iron levels in E. coli. Although E. coli cells respond to low intracellular iron during nickel stress by maintaining high expression of iron uptake systems such as fepA, the demand for iron is not met due to a lack of siderophores in the extracellular medium during nickel stress. Taken together, these results indicate that nickel inhibits iron accumulation in E. coli by reducing the presence of enterobactin in the extracellular medium. INTRODUCTION Nickel exposure can cause allergies and lung cancer in humans (Macomber and Hausinger, 2011; Denkhaus and Salnikow, 2002; Kasprzak et al., 2003; Das et al., 2008).
Response relationships between CO2, CH4 and N2O emissions and microbial functional groups in wetland sediments after trace metal addition

Biochemical nitrogen removal relies heavily on wetland microbes, prior to entering downstream ecosystems. Microbes utilize oxy-reductases that contain metal-cofactors to reduce NO 3 and NO 2 to N 2 . These transformations are linked also to other C cycling processes and may emit N 2 O, CH 4 and CO 2 as well. In this incubation study we tested the effects of supplementing wetland sediments with trace-metals (Cu, Mo, Fe) at μM levels on nitrogen kinetics, gaseous emissions (N 2 O, CO 2 and CH 4 ) and genes abundances for the overall microbial community, related to NO 2 reduction (denitrification and DNRA), NO 2 reduction and methanogenesis. Trace metal availability and specifically Cu, enhances the reduction of N 2 O to N 2 , increases the production of CH 4 and has no effect on CO 2 emissions from wetland sediments. When sediments were supplied with Cu, higher gene abundances were observed for Cu-reductases nirK and nosZ . Mo addition increased CH 4 emissions from sediments and higher mcrA gene abundances were observed. Emerging from this investigation, trace-metals bio-availability may directly or indirectly regulate denitrification in the environment; an effect observed so far only in pure microbial cultures.
La pratique d’activités de loisir par des enfants d’âge scolaire porteurs d’implants cochléaires : description de l’expérience des parents

La pratique d’activites de loisirs chez les enfants ayant une surdite, particulierement chez ceux qui ont recu un implant cochleaire, n’a ete pour ainsi dire jamais examinee jusqu’ici. En effet, la majorite des etudes portent sur les enfants ayant des incapacites motrices, un trouble du spectre de l’autisme ou une deficience intellectuelle. Dans cette etude, dix-neuf parents d’enfants porteurs d’implants cochleaires ont participe a des entrevues individuelles visant a comprendre leur experience reliee a la pratique d’activites de loisirs par leur enfant. Les resultats revelent que les parents sont generalement favorables a la pratique d’activites de loisirs et estiment que leur enfant y reussit bien. Par contre, pres du tiers des parents ont exprime des craintes face a la pratique de certaines activites, particulierement celles qu’ils croient etre dangereuses pour l’appareil porte par leur enfant. Les resultats font ressortir l’importance de la sensibilisation a la surdite et a l’implant cochleaire dans tous les milieux ou se pratiquent les activites de loisirs.
Effect of Dietary Supplementation of Yeast on Growth, Feed Conversion Efficiency and Cost of Feeding in Surti Goat Kids

Small ruminant farming is still the major chunk of livelihood among the small and marginal farmers in India. Agricultural byproducts and crop residues represents a potential source of dietary nutrients for these animals. These resources are generally deficient in readily available source of energy, protein and other micronutrients. More over the high fiber content of these feed resources restrict the accessibility of ruminal microbes thereby their digestibility (Tan et al., 1995). In recent years, use of probiotics has been used to manipulate the rumen microbial ecosystem to enhance the nutritive value and utilization efficiency of low-quality roughages (Tang et al., 2008). Among the different microbial feed additives, Saccharomyces cerevisiae and Aspergillus oryzae are more effective in rumen, whereas lactobacilli are effective during pre-ruminant stage (Khuntia and Chaudhary, 2002). Yeast culture supplementation in ruminant diets can increase dry matter intake (DMI), production performance, cellulose degradation, and nutrient digestibility (Lesmeister et al., 2004). In vitro studies have also shown that yeast culture favorably modified the mixed ruminal microorganism fermentation and stimulated International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 09 (2018) Journal homepage: http://www.ijcmas. com
Time and Area Optimized Testing of Automotive ICs

As cars become increasingly computerized and their safety functions evolve rapidly, the number of complex safety-critical components deployed in advanced driver assistance systems or autonomous vehicles is rising dramatically with high-end models containing hundreds of embedded microcontrollers. These integrated circuits must adhere to stringent requirements for high quality and long-term reliability driven by functional safety standards. This requires test solutions that address challenges posed by automotive systems. This article presents a scan-based test scheme optimizing test time and area overhead during manufacturing and in-system test of automotive electronics. The proposed scheme deploys observation test points that capture the faulty effects in every shift cycle into separate observation scan chains. To reduce area overhead, the scheme enables the sharing of flip-flops among control points. It is also shown how test points enhance test coverage (TC) in the presence of cascaded clock gaters. Finally, processing challenges when fault simulating every scan shift cycle to determine TC are addressed. Experimental results obtained for contemporary automotive designs and reported herein show significant improvements in test quality over traditional solutions.
Optimal Concurrent Control for Space Manipulators Rendezvous and Capturing Targets Under Actuator Saturation

This article proposes a control method for space manipulators rendezvousing with and capturing a target, involving concurrent operation of an optimal and a coordinated controller. The optimal controller drives the whole system to rendezvous with the target, which saves onboard fuel and satisfies obstacle avoidance constraint and base spacecraft thrust limits. The optimal control problem is solved using calculus of variations method, with state inequality constraints transformed into extended dynamical subsystems and thrust limits formulated as saturation functions. The coordinated controller is designed based on the dynamic equations of the space manipulator on a noninertial frame attached to the system center of mass, which drives the end-effector to approach the target along a desired trajectory while making the base attitude follow a desired profile. It also generates proper reaction moments on base through manipulator motions to ensure controlling the base attitude when base attitude actuators reach their torque limits. The solution optimality of the optimal controller and the stability of the coordinated controller are demonstrated. Numerical simulations verify the performance of the proposed concurrent control method.
In vitro antimicrobial and antioxidant activity of some berry species

Dietary patterns characterized by relatively high intakes of fruits and vegetables are consistently associated with reductions in the incidence of noncommunicable diseases such as coronary heart disease, stroke, cancer, and various chronic disease1. Berries provide significant health benefits because of their high levels of polyphenols, antioxidants, vitamins, minerals, and fibers2. Most berries are ABSTRACT
Genome-scale Evaluation of the Biotechnological Potential of Red Sea Bacilli Strains

Genome-scale Evaluation of the Biotechnological Potential of Red Sea Bacilli Strains Ghofran Othoum The increasing spectrum of multidrug-resistant bacteria has caused a major global public health concern, necessitating the discovery of novel antimicrobial agents. Additionally, recent advancements in the use of microbial cells for the scalable production of industrial enzymes has encouraged the screening of new environments for efficient microbial cell factories. The unique ecological niche of the Red Sea points to the promising metabolic and biosynthetic potential of its microbial system. Here, ten sequenced Bacilli strains, that are isolated from microbial mat and mangrove mud samples from the Red Sea, were evaluated for their use as platforms for protein production and biosynthesis of bioactive compounds. Two of the species (B. paralicheniformis Bac48 and B. litoralis Bac94) were found to secrete twice as much protein as Bacillus subtilis 168, and B. litoralis Bac94 had complete Tat and Sec protein secretion systems. Additionally, four Red Sea Species (B. paralicheniformis Bac48, Virgibacillus sp. Bac330, B. vallismortis Bac111, B. amyloliquefaciens Bac57) showed capabilities for genetic transformation and possessed competence genes. More specifically, the distinctive biosynthetic potential evident in the genomes of B. paralicheniformis Bac48 and B. paralicheniformis Bac84 was assessed and compared to nine available complete genomes of B. licheniformis and three genomes of B. paralicheniformis. A uniquely-structured trans-acyltransferase (transAT) polyketide synthase/nonribosomal peptide synthetase (PKS/NRPS) cluster in strains of this species was identified in the genome of B. paralicheniformis 48. In total,
MACSen: A Processing-In-Sensor Architecture Integrating MAC Operations Into Image Sensor for Ultra-Low-Power BNN-Based Intelligent Visual Perception

Current BNN-based visual system wastes lots of energy in data conversion and movement, hindering its deployment on battery-powered devices. This brief proposes MACSen, an ultra-low-power processing-in-sensor (PIS) architecture which integrates sensing with computing and directly outputs the computation results. The multiply-and-accumulation (MAC) operation in BNN is fused with the Correlated Double Sampling (CDS) procedure together to save data conversion power. A $4\times 4$ MACSen prototype of 180nm process was fabricated for demonstration, and it achieves the frame rate of 1000fps and the energy efficiency of 1.32TOP/s/W in computation mode. Furthermore, the system demonstration on MNIST dataset classification task shows that the hardware BNN implementation integrating MACSen incurs no accuracy degradation and gains 61% energy saving compared with state-of-the-art work.
The plant immune system : induction, memory and de-priming of defense responses by endogenous, exogenous and synthetic elicitors

En tant qu’organismes sessiles, les plantes doivent reagir rapidement et intensement, via des reponses defensives, pour repousser les pathogenes invasifs. Le systeme immunitaire des plantes peut etre declenche par des molecules elicitrices exogenes ou endogenes. Une autre classe d’eliciteurs, les eliciteurs synthetiques, contient egalement des composes promouvant une reponse defensive.Dans ce manuscrit, je decris la decouverte et caracterisation d’une nouvelle famille de petits peptides endogenes potentiellement secretes(PROSCOOP), dont les membres incluent de petits peptides (SCOOP). Je demontre que les SCOOP sont impliques dans les mecanismes de defense de la plante et le developpement racinaire. Une variete de peptides SCOOP induit des reponses defensives de courtes et longues durees.De plus, des traitements avec le peptideSCOOP12 induisent une resistance a Pseudomonas syringae chez Arabidopsis.Dans la seconde partie de cette these, je demontre que le traitement des plantes avec un eliciteur synthetique peut mener a une memoire transcriptionnelle a long terme, et que le challenge subsequent des plantes traitees par application d’un eliciteur exogene desactive cette memoire transcriptionnelle. En conclusion, ma these presente (1) la diversite des fonctions que peuvent avoir ces eliciteurs et (2) l’impact sur les systemes de defense de la plante et ses consequences sur la memoire et le developpement de la plante.
Genetic Studies on Growth and Production Traits in Rambouillet Sheep

Data on 300 Rambouillet sheep records maintained at Government Sheep Breeding Farm Panthal, Reasi, JandK, India, were used in the present study. The least squares means were 2. 608Â±0.034 kg, 15. 846Â±0.179 kg, 17. 921Â±0.164 kg, 22. 890Â±0.205 kg, 30. 463Â±0.172 kg and 2. 121Â±0.042 kg birth weight (BWT), weaning weight (WWT), 6-month body weight (6-BW), 12-month body weight (12-BW), mature body weight (MBW) and annual wool production (AWP), respectively. The period of lambing had significant effect on all the traits except for BWT. Sex had significant effect on all the traits under study except for 6-BW. The estimates of heritability for the present study in Rambouillet sheep varied from low to medium. The genetic correlation in Rambouillet sheep varied from -0. 786Â±0.630 (MBW and AWP) and 0. 915Â±0.336 (WWT and MBW). The moderate estimates of heritability of 6-BW and its high genetic correlation with MBW indicated that 6-BW can be used as selection criteria for MBW.