Miracle-dz/UniFAR-FTUs huggingface dataset:
Tissue and blood protozoa including toxoplasmosis, Chagas disease, leishmaniasis, Babesia, Acanthamoeba, Balamuthia, and Naegleria in solid organ transplant recipients— Guidelines from the American Society of Transplantation Infectious Diseases Community of Practice

These updated guidelines from the Infectious Diseases Community of Practice of the American Society of Transplantation review the diagnosis, prevention, and management of tissue and blood protozoal infections in the pre‐ and post‐transplant period. Significant new developments in the field have made it necessary to divide the previous single guideline published in 2013 into two sections, with the intestinal parasites separated from this guideline devoted to tissue and blood protozoa. The current update reflects the increased focus on donor screening and risk‐based recipient monitoring for parasitic infections. Increased donor testing has led to new recommendations for recipient management of Toxoplasma gondii and Trypanosoma cruzi. Molecular diagnostics have impacted the field, with access to rapid diagnostic testing for malaria and polymerase chain reaction testing for Leishmania. Changes in Babesia treatment regimens in the immunocompromised host are outlined. The risk of donor transmission of free‐living amebae infection is reviewed. Changing immigration patterns and the expansion of transplant medicine in developing countries has contributed to the recognition of parasitic infections as an important threat to transplant outcomes. Medications such as benznidazole and miltefosine are now available to US prescribers as access to treatment of tissue and blood protozoa is increasingly prioritized.
A Feasibility Study for Diagnosis of Latent Tuberculosis Infection Using an IGRA Point-of-Care Platform in South Korea

Purpose This study aimed to evaluate ichroma™ IGRA-TB, a novel point-of-care platform for assaying IFN-γ release, and to compare it with QuantiFERON-TB Gold In-Tube (QFT-GIT) for identifying Mycobacterium tuberculosis (M. tb) infection. Materials and Methods We recruited 60 healthy subjects, and blood samples were obtained in QFT-GIT blood collection tubes. The blood collection tubes were incubated at 37℃, and culture supernatant was harvested after 18–24 hours. IFN-γ responses were assessed by the ichroma™ IGRA-TB cartridge and the QFT-GIT IFN-γ enzyme-linked immunosorbent assay. Three active TB patients were recruited as a positive control for M. tb infection. Results The area under the receiver operating characteristic curve of the ichroma™ IGRA-TB test for differentiating between infected and non-infected individuals was 0.9706 (p<0.001). Inconsistent positivity between the two tests was found in three participants who showed weak positive IFN-γ responses (<1.0 IU/mL) with QFT-GIT. However, the two tests had excellent agreement (95.2%, κ=0.91, p<0.001), and a very strong positive correlation was observed between the IFN-γ values of both tests (r=0.91, p<0.001). Conclusion The diagnostic accuracy demonstrated in this study indicates that the ichroma™ IGRA-TB test could be used as a rapid diagnostic method for detecting latent TB infection. It may be particularly beneficial in resource-limited places that require cost-effective laboratory diagnostics.
Antioxidant Activity of Collagen Extracts Obtained from the Skin and Gills of Oreochromis sp.

Efforts aimed at reduction of fishing waste generated during the evisceration and filleting are scarce. The fishing waste is used in the production of low value-added products, such as flours or silages. It is important to visualize an alternative and profitable use of this waste, as it constitutes a serious environmental problem. This research determined the antioxidant properties of collagenous extracts of skin and galls of Oreochromis sp. The raw materials were characterized by proximal chemical analysis. Three treatments were applied to extract the collagen: salt-soluble collagen, acid-soluble collagen (ASC), and pepsin-hydrolyzed collagen (PHC). The collagenous fractions were hydrolyzed (0.1% pepsin). The recovered collagen yield and antioxidant activity were determined to hydrolyzed collagen (HC) and nonhydrolyzed collagen (NHC). The ASC skin showed the highest extraction yield (3.02%). For galls, only the PHC extraction was feasible (0.16%). Antioxidant analysis of NHC did not reveal radical scavenging activities. HC displayed a 2,2-diphenyl-1-picrylhydrazyl %RSA of 22.58 (ASC skin) and 10.34% (PHC galls), and a 2,2'-azino-bis[3-ethylbenzothiazoline-6-sulfonic acid] %RSA of 30.40% (PHC skin) and 29.43% (PHC galls), respectively. The ASC skin and PHC gall extracts exhibited 94.40% and 81.54% in ferric-reducing antioxidant power, and 43.63 and 38.08 μg ascorbic acid equivalents per milli liter for total antioxidant capacity, respectively. The collagen extracts showed %RSA and chelation of pro-oxidant metal ions. Different mechanism of antioxidant action was identified for the extracts: radical scavengers for HC and metal ion chelators for NHC. In conclusion, red tilapia skin collagen is recommended as an active ingredient of nutraceuticals, pharmaceuticals, or functional foods, for the identified bioactive properties.
Cross-sectional study estimating the psychosocial impact of genital warts and other anogenital diseases in South Korea

Objectives To estimate self-reported human papillomavirus (HPV) disease-related psychosocial impact among male and female patients in South Korea. Design In this multicentre cross-sectional study, psychosocial impacts were estimated using a one-time survey capturing HPV Impact Profile (HIP) results, CuestionarioEspecifico en Condilomas Acuminados (CECA; in Spanish)—‘Specific questionnaire for Condylomata Acuminata’ and the EuroQol-5 Dimension (EQ-5D) surveys. Student’s t-tests or Mann-Whitney U tests were used for continuous comparisons; χ2 or Fisher’s exact tests were applied for categorical comparisons. Setting 5098 clinics throughout Seoul, Busan, Daegu, Kwangju and Daejeon (South Korea). Participants Patients with and without genital warts (GW) (males) and selected HPV diseases (females) visiting primary care physicians, obstetricians/gynaecologists, urologists and dermatologists with 2–30 years experience. Results Of 150 male and 250 female patients, HIP scores showed 85.3% of male patients with GW and 32.0% without reported moderate psychological impact (p<0.0001). In categorised total scores, 88.5% of female patients with and 66.0% without selected HPV-related diseases reported moderate or high psychological impacts (p=0.0004). In the CECA questionnaire, male patients had mean (SD) scores of 10.51 (3.79) in ‘emotional health’ and 15.90 (6.13) in ‘sexual activity’. Female patients with GW reported lower scores in both dimensions with mean scores of 7.18 (4.17) in ‘emotional health’ and 10.97 (5.80) in ‘sexual activity’ (p<0.0001), indicating worse health-related quality of life (HRQoL). For the EQ-5D, male patients with GW reported lower mean Visual Analogue Scale (VAS) scores than those without (75.1 vs 81.13, p<0.0135). Mean VAS score and utility values were lower for females with HPV-related diseases than those without (72.18 vs 76.86 and 0.90 vs 0.94, respectively). Conclusion In South Korea, GW in men and HPV-related diseases in women negatively impact patient well-being and HRQoL scores. Among women, those with GW suffered a greater psychosocial impact than those with other selected HPV-related diseases.
Lymphatic micrometastases predict biochemical recurrence in patients undergoing radical prostatectomy and pelvic lymph node dissection for prostate cancer

Abstract Background Nodal metastasis is a strong prognostic parameter in prostate cancer (PCa). We analysed the detection of micrometastases (miN + ) in initially nodal-negative (pN0) radical prostatectomy specimens from pT2a-c and pT3a PCa patients by immunohistochemistry (IHC). Material and Methods A total of 2352 lymph nodes of 193 PCa patients were centrally re-examined for miN + or miN- status using IHC. Results were correlated with clinical and follow-up data. Recurrence-free survival (RFS) was calculated with the log-rank test using the Kaplan-Meier method. In addition, a logistic regression analysis was performed. Results IHC detected miN + in a total of 17 patients (8.8 %). miN + seemed to be significantly associated with a higher Gleason score and was detected in more advanced pT stages. A total of 45 patients (23.1 %) had a biochemical recurrence (BCR). BCR was associated with miN +. Patients with miN + had a significantly shorter RFS (22.9 versus 58.7 months; p < 0.001). In the univariate (OR: 5.04; 95 % CI: 2.46 – 10.6; p-value: < 0.0001) and multivariate (OR: 3.29; 95 % CI: 1.54 – 7.08; p-value: 0.002) regression model, the miN + status was the strongest predictor of a BCR. Conclusions IHC seems to be of high diagnostic value for the detection of micrometastases in initially nodal-negative PCa patients. IHC should therefore be performed in PCa patients with nodal-negative findings.
Qualitative, exploratory pilot study to investigate how people living with posterior cortical atrophy, their carers and clinicians experience tests used to assess vision

Objectives To investigate the experiences and views of people living with posterior cortical atrophy (PCA), their family carers and healthcare professionals of vision assessment tests. Design A qualitative investigation using video recordings of vision assessments, semistructured interviews and audio recordings of a focus group. Interviews and focus group used broad, open questions around the topic to prompt and guide discussion. Video and audio recordings were transcribed, manually coded and analysed using framework analysis. Setting University College, London’s Queen Square neurology centre provided the venues for all stages of the research. Participants Participants living with PCA were one male and two females (age range 67–78 years). Health professional participants were a neurologist (male), two ophthalmologists (male) and an optometrist (female). Primary and secondary outcomes (1) Experiences and attitudes of people living with PCA and health professionals to vision assessment tests, (2) views of health professionals and people living with PCA of whether some tests are more effective at discriminating between cortical vision problems and vision problems related to optical or ocular causes. Results Patients were able to engage with and complete a number of tests. Their partners played a vital role in the process. Participants reported that simple, short tests were more effective than more subjective tests. Examples of tests that appeared to be more problematic for the patient participants were the Amsler Grid and visual field analysis. Conclusions Although limited in scope and execution, the project suggests that some vision assessment tests are likely to support health professionals to discriminate between cortical and optical/ocular causes of visual impairment. It supports existing evidence that there are vision assessments that people with dementia can engage with and complete. We identify areas of importance for future research and make tentative suggestions for clinical practice.
An approach using a novel phage mix for detecting Pseudomonas aeruginosa in water

The present study aims to integrate the benefits of plaque assay using a novel phage mix with phylogenetic and molecular analysis for detecting Pseudomonas aeruginosa in water. Three phages were isolated and the transmission electron microscope related their morphological resemblance to those of Siphoviridae and Podoviridae families, while molecular analysis showed different cp‐gene sizes. The Phage mix was highly specific (86.0%), and data misleading didn't exceed 14.0% compared to membrane filter assay (39.2%). Time elapsed for test completion required 24 h. Identified P. aeruginosa were verified using 16S‐rDNA. Nucleotide sequence data for both phages and bacteria were submitted to the NCBI GenBank database, USA and gained their accession numbers. Concluding remarks highlight the potential of plaque assay as specific, simple and rapid method. The study recommended future efforts to isolate and characterize new phages for detecting other bacterial pathogens of public health concern to control water pollution and maintain adequate hygiene.
Migration and Phenotype Control of Human Dermal Fibroblasts by Electrospun Fibrous Substrates

Electrospun fibrous matrices, mimicking extracellular matrix (ECM) hierarchical structures, are potential scaffolds for wound healing. To design functional scaffolds, it is important to explore the interactions between scaffold topographic features and cellular responses, especially directional migration and phenotypic changes, which are critical functional aspects during wound healing. Here, accelerated and persistent migration of human dermal fibroblasts (HDFs) is observed on fibers with aligned orientation. Furthermore, aligned fibers can induce fibroblast‐to‐myofibroblast differentiation of HDFs. During wound healing, the presence of myofibroblasts advances wound repair by rendering contractile force and ECM deposition within the early and middle courses, but its continuous persistence in the later event may not be desired due to the contribution in pathological scarring. To tune the balance, it is noted in this work that the introduction of matricellular protein angiopoietin‐like 4 (ANGPTL4) is capable of reversing the phenotypic alteration induced by aligned fibers, in a time‐dependent manner. These results indicate fibrous matrices with oriented configuration are functional in mediating directional cell migration and phenotypic change. The discoveries further suggest that tissue‐engineered fibrous grafts with precise alignment modulation and ANGPTL4 releasing properties may thus be promising to promote wound repair with minimizing scar formation.
Green Supply Chain Management as Antecedent of Green Satisfaction: Examining Sequential Mediation of Green Marketing and Green Corporate Image

The main objective of this study is to examine the impact of green supply chain management, green marketing, and image on the Green satisfaction of dairy industry of Indonesia. Furthermore, the sequential mediation of green marketing and image is examined in the present study as well. Data is collected from 550 the customers of dairy products through surveys. The response rate of data collection was 57%. In order to test the hypothesis and analysis of the data, in this study for data analysis we have adopted the Partial Least Squares Structural Equation Modelling (PLS-SEM) approach. All of the proposed hypothesis of the present study has proved to be significant showing that image, green marketing and green supply chain management practices of organization are important for the industry to gain the Green satisfaction because customers are more environmental conscious. The findings of the study are important for the policy makers of dairy milk sectors to derive the policies to satisfy the customers. Moreover, the present study also fills the gap of limited studies regarding the Green satisfaction in supply chain context.
Prevalence of diabetes in liver cirrhosis: A systematic review and meta‐analysis

An association between diabetes mellitus (DM) and liver cirrhosis is well‐known, but estimates of the prevalence of DM in patients with liver cirrhosis vary widely. A systematic review was undertaken to determine the prevalence of DM in adult patients with liver cirrhosis. The Medline, EMBASE, and Cochrane Library databases were searched for peer‐reviewed studies published in English (1979‐2017) that investigated the prevalence of diabetes in adult patients with cirrhosis. Pooled estimates of prevalence of DM were determined for all eligible patients and according to aetiology and severity of liver disease. Fifty‐eight studies satisfied criteria for inclusion, with 9705 patients included in the pooled prevalence analysis. The overall prevalence of DM was 31%. The prevalence of DM was highest in patients with nonalcoholic fatty liver disease (56%), cryptogenic (51%), hepatitis C (32%), or alcoholic (27%) cirrhosis. For assessing prevalence of DM as a function of severity of liver disease, evaluable data were available only for hepatitis C and hepatitis B cirrhosis. DM may be more prevalent in cirrhosis than previously thought. This has implications for prognosis and treatment in these patients.
Portable fixed dynamometry to quantify ankle dorsiflexion force

Introduction: Quantifying muscle strength is critical in clinical and research settings. A rapid and objective method is ideal. The primary objective of this study was to examine the reliability of a novel device, the rapid objective quantification― tibialis anterior (ROQ‐TA), which quantifies the dorsiflexion force of the tibialis anterior, and to assess its validity against isokinetic dynamometry (IKD). Methods: Ankle dorsiflexion of 20 healthy subjects was assessed by 3 modalities, ROQ‐TA, manual muscle testing, and isokinetic dynamometry, over 2 testing sessions. Results: The intraclass correlation coefficient [ICC(2,1)] for reliability was 0.872 (0.677–0.949) for the ROQ‐TA and 0.892 (0.728–0.957) for IKD. For validity, the ICC(2,1) values for the ROQ‐TA and IKD were in good agreement, with 0.672 (0.17–0.87) in the first testing session and 0.769 (0.42–0.91) in the second session. Discussion: The ROQ‐TA is a valid and reliable device to test ankle dorsiflexion force in a healthy population. Muscle Nerve, 2018
Efficiency of health risk mitigation: complex assessment based on fuzzy sets theory and applied in planning activities aimed at ambient air protection

When industrial objects emitting substantial masses of dust and gas mixtures are located within a settlement or close to its borders, it often results in poorer quality of the environment and damages to population health. Such a situation is typical for many cities in the country; primarily, for those that are included into “Pure air” Federal project, a part of the “Ecology” National project. Negative effects are produced by a set of various substances emitted from various industries. And it is quite often that great numbers of people are exposed to such emissions and as a result multiple and variable responses from their health are registered. Assessment of share contributions made by different emissions sources and each particular substance into aggregated negative responses from human health is a fundamental stage in assessing damages to health that occurred due to them; it is significant for working out an action plan aimed at hazardous impacts mitigation. Given that, we proposed an approach based on fuzzy sets theory as a relevant methodological basis for assessing efficiency of risk mitigation and damage to health when planning and implementing activities aimed at ambient air protection. Application of this methodology allows assessing conditions of multi-component negative impacts producing multiple negative effects including direct damage done to human health. And here key parameters are assessed not as per point values but as per interval ones that are characterized with their belonging to a range of scaled parameters. Our research goal was to suggest methodical approaches to assessing efficiency of risk mitigation and damage to health when planning and implementing activities aimed at ambient air protection; the approaches were based on fuzzy sets theory. Results obtained via hygienic (field or calculated examinations of ambient air quality in settlements under exposure and beyond it) and epidemiologic (controlled medical and biological) research are taken as initial data for fuzzy modeling of multiple parameters ratios within “damage to health – mitigation efficiency” system. Principles applied for research design take into account key postulates of exposure assessment, “dose – effect” relationship for an influencing substance, a concept of exposure risk acceptability, peculiarities related to body reactions under combined aerogenic burdens, and plans for ambient air protection activities (including complex ones). Comparing a list of substances that do actual damage to exposed population’s health with a list of substances included into plans on aggregated emissions reduction allows assessing adequacy; determining to what extent damage to health is mitigated allows assessing whether activities aimed at ambient air protection are sufficient and effective.
An Older and Exceptionally Large Adult Specimen of Tyrannosaurus rex

Here we describe an extremely large and relatively complete (roughly 65%) skeleton of Tyrannosaurus rex (RSM P2523.8). Multiple measurements (including those of the skull, hip, and limbs) show that RSM P2523.8 was a robust individual with an estimated body mass exceeding all other known T. rex specimens and representatives of all other gigantic terrestrial theropods. Histological sampling of the fibula confirms that RSM P2523.8 is skeletally mature. The prevalence of incompletely coossified elements contradicts previous assertions that such unfused elements can be taken as indicators of somatic immaturity. As an extreme example of both ontogenetic maturity and osteological robustness, RSM P2523.8 offers support for prior hypotheses that a sampling bias occurs throughout the Dinosauria, making it likely that most taxa grew to significantly greater size than current known specimens indicate. Anat Rec, 303:656–672, 2020. © 2019 Wiley Periodicals, Inc.
Comparing the Effect of Dexamethasone, Normal Saline, and Metoclopramide on Prevention of Postoperative Nausea, Vomiting and Pain in Patient Undergoing Laparoscopic Cholecystectomy or Open Appendectomy: A Randomized Clinical Trial

BACKGROUND: Post-operative nausea and vomiting (PONV) and pain are a common complications of adult patients undergoing anesthesia, but the incidence of these complications in patients go through laparoscopic cholecystectomy or open appendectomy is unknown. AIM: We conduct this study for comparing the effect of dexamethasone administration with normal saline and with metoclopramide for reducing these undesirable effects. DESIGN: This was a three-armed, parallel-group, double-blind, and randomized controlled clinical trial. METHODS: Seventy-two patients of both genders underwent elective surgeries (laparoscopic cholecystectomy or open appendectomy), randomly assigned to three groups: A dexamethasone-treated (8 mg) group (Group 1) (n = 24), normal saline-treated (100 ml) group (Group 2) (n = 24), and metoclopramide-treated (10 mg) group (Group 3) (n = 24). All nausea, vomiting, and pain episodes were recorded during 24 h after anesthesia in three time periods: 0–4, 4–12, and 12–24 h post-anesthesia. Results: Nausea and vomiting grading scale shows no significant difference when compare dexamethasone (Group 1) with normal saline (Group 2), significant difference when compare dexamethasone (Group 1) with metoclopramide (Group 3), and also significant difference when compare normal saline (Group 2) with metoclopramide (Group 3). Group 1 showed a lower pain score (p < 0.01 and p < 0.0001) compared with pain score recorded by patients of Groups 2 and 3, respectively. Besides that, Group 2 also showed a lower pain score (p < 0.0001) compared with pain score recorded by Group 3 patients. CONCLUSION: Dexamethasone has unimportant effect when compare with normal saline, more effectiveness than metoclopramide in reducing PONV. However, dexamethasone more effective in reducing pain when comparing with normal saline and metoclopramide for patients they underwent laparoscopic cholecystectomy or open appendectomy surgery under general anesthesia.