Miracle-dz/UniFAR-FTUs huggingface dataset:
Implementacja metody the St. Gallen Model for Destination Management (SGDM) w polskich Karpatach: Studium przypadku sześciu bieszczadzkich gmin

Bieszczady są jednym z pasm górskich leżących w polskich Karpatach. Ich walory przyrodnicze i kulturowe przyciągają corocznie do tej destynacji tysiące polskich i zagranicznych turystów. Pomimo wielu objętych ochroną miejsc o szczególnych walorach przyrodniczych (np. Bieszczadzki Park Narodowy, Rezerwat Biosfery UNESCO i obszary Natura 2000) Bieszczady borykają się obecnie z różnymi problemami środowiskowymi i społecznymi, które często wynikają z dynamicznego, ale chaotycznego rozwoju turystyki na ich obszarze. Brak wspólnej wizji długofalowego rozwoju turystyki (na poziomie gminnym i regionalnym) stanowi jedno z głównych wyzwań, przed którym stoją obecnie podmioty publiczne i prywatne wielu gmin bieszczadzkich. W artykule zaprezentowano rezultaty wdrożenia innowacyjnej metody zarządzania destynacjami – The St.Gallen Model for Destination Management (SGDM) – w wybranych bieszczadzkich gminach. Zastosowana metoda umożliwiła lokalnym „aktorom” turystycznym (publicznym i prywatnym) przystąpienie do procesu holistycznego zarządzania miejscami recepcji turystycznej poprzez identyfikację kluczowych segmentów rynku. W pracy przedstawiono pierwszą próbę zastosowania metody SGDM w Polsce.
The case for constructionist, longitudinal and ethnographic approaches to understanding event experiences

ABSTRACT This paper argues that to really understand the complexities of event experiences and their meaning, we need to gather rich data, on a longitudinal basis. It demonstrates how ethnographic and constructionist approaches assist in understanding event experiences in relation to the cultural context, symbolic nature, and ritualistic aspects of the event and the corresponding impacts on participants. It considers how spending time immersed in the culture of the event and observing with a wide angle lens, using photographic evidence to capture, recall and discuss experiences, provides for a depth of data beyond the realms of quantitative data collection. The paper presents research undertaken at the case of Llangollen International Musical Eisteddfod into experiences by event attendees and volunteers in relation to the specific aspect of intercultural communication and exchange. The findings provided rich and meaningful data on individual experiences at the case study event from which to provide recommendations for organisers on how this aspect of the event can be enhanced and improved. The results underline and demonstrate the effectiveness of longitudinal, constructionist and ethnographic methodological approaches in understanding event experiences, and their credibility and generalisability moving into the future.
Astrocytes and Inflammatory Processes in Alzheimer’s Disease

A significant increase in inflammation has been shown to be a crucial factor in the progression of the Alzheimer’s disease (AD). Moreover, inflammatory signals are already present in mild cognitive impairment (MCI) patients before they develop AD. The amyloid hypothesis argues that in AD, there is an increase in oxidative stress caused by the accumulation of β -amyloid (A β ) and that its elimination should be a priority. Also, hyperphosphorylation of the protein TAU occurs, which is characteristic of this disease. In AD oxidative stress processes occur and also inflammation. The basal chronic inflammation produces a cascade of cellular, such as astrocytes and microglial cells, and molecular processes in AD patients. We here have tried to explore the action of the inflammatory process and its implication in the neurodegenerative process of the AD. We can see that the role of A β is only one component that gives rise to inflammation, probably mediated by activation of microglia and astrocytes with the goal of getting rid of these brain waste products. In fact, it is related to a greater degree with the progression of the disease and worsening of the symptoms with the increase of phosphorylated TAU in different parts of the brain.
Phenotypic Responses to Invasion in the Brown Anole (Anolis sagrei)

Invasive species often encounter climatic conditions that differ significantly from those of their native range. These environmental shifts may trigger phenotypic responses, resulting through some combination of adaptation and plasticity, that enable the invader to persist under novel thermal regimes. In this dissertation, I examine phenotypic changes in a tropical lizard that has successful invaded a cooler temperate climate, specifically examining traits that may promote survival and reproduction in their new range. First, I examined physiological traits, as I predicted greater cold tolerance would be necessary to survival in the invasive range. I found that invasive populations tolerated lower temperatures, exhibited greater maximum sprint speeds, and had higher metabolic rates than native populations. Next, I examined how life-history traits may change in the invasive range in order to facilitate reproduction under shorter breeding and growing seasons. I found that compared to native females, invasive females had shorter interlaying intervals and produced eggs that hatched more quickly. Once I quantified changes physiological and life-history traits that may have aided in successful establishment, I executed a common garden study to determine whether changes were the result of adaptation or plasticity. I found that differences in critical thermal minimum, metabolic rate, interlaying interval, and incubation period were maintained in lab-reared offspring, while measures of sprint speed converged. My results provide evidence that life history and physiology can evolve rapidly during invasion. These findings are useful to understanding contemporary evolution, and also provide valuable insight on how species respond to environmental shifts, both during invasions and as a result of climate change.
Palliative Care as the Driving Force for Providing Psychological Comfort to Patients with Cancer: A Hermeneutic Study

Background: Palliative care is an important part of eective cancer care Objectives: The purpose of our study was to explore the lived experiences of nurses providing psychological palliative care for cancer patients in Iran. Methods: A hermeneutic phenomenological study was performed. We interviewed 11 oncology nurses to understand their lived experiences in cancer wards about psychological palliative care. We employed a seven-stage process of data analysis. Results: One constitutive pattern 'palliative care as the driving force for providing psychological comfort to patients with cancer' and three associated themes, namely 'stress management', 'empathetic behavior', and 'all-embracing outlook', were identified. Conclusions: The findings present better understanding of the lived experiences of oncology nurses in Iran regarding the phenomena under study.
Research of Chinese Government debt sustainability and its effect on economic growth

This paper researches the appropriate scale of Chinese government debt from the perspective of debt sustainability and its effect on economic growth. First, we made a model based on fiscal response framework and introduced trade openness, savings ratio and so on as control variables. We use it to measure the upper limit of the sustainable debt ratio over the years. Then we did empirical research and found that government debt has a threshold effect on economic growth, with a turning point of about 33.3%. Besides, we also found that debt affects economy by affecting the formation of total society capital significantly, and excessive debt may inhibit the formation. According to our research, the government should release savings reasonably, improve its sensitivity to debt and use it more efficiently to enhance the turning point of debt impact. Government should also gradually reduce current debt ratio to optimize the relative scale of debt.
Wide-Area Backup Protection Against Asymmetrical Faults Using Available Phasor Measurements

This paper proposes a robust and computationally efficient wide-area backup protection (WABP) scheme against asymmetrical faults on transmission systems using available synchronized/unsynchronized phasor measurements. Based on the substitution theorem, the proposed scheme replaces the faulted line with two suitable current sources. This results in a linear system of equations for WABP, with no need of full system observability by measurement devices. The identification of the faulted line is attributed to the sum of squared residuals (SoSR) of the developed system of equations. To preserve accuracy, the scheme limits the calculations to the assessment of the negative-sequence circuit of the gird. Relevant practical aspects that have not been properly addressed in the literature, namely the non-simultaneous opening of circuit breakers (CBs) and their single-pole tripping for single-phase to ground faults are investigated. The linearity of the formulations derived removes concerns over convergence speed and potential time-synchronization challenges. The proposed scheme is able to identify the faulted line and retain this capability for hundreds of milliseconds following the fault inception. More than 20 000 simulations conducted on the IEEE 39-bus test system verify the effectiveness of the proposed WABP scheme.
Nutrients and non-nutrients composition and in vitro starch digestibility of five Algerian legume seed flours

The authors wish to express their sincere thanks to the University of Abderrahmane Mira, Algeria, the Research Centre of Agriculture and Agri-Food, Canada, and the Department of Physiology andBiochemistry of Animal Nutrition (EEZ, CSIC) Spain. They are also equally grateful to McIntoch, T. , Marambe, H. , Peinado, M. J., Ruiz, R. and Hueso, R.
Dietary Glycocalyx Precursor Supplementation Ameliorates Age‐Related Vascular Dysfunction

Large elastic artery stiffening and endothelial dysfunction, and associated reductions in nitric oxide (NO) bioavailability, are central features of vascular aging. We have recently demonstrated that the glycocalyx, a gel‐like structure that is bound to the luminal surface of the vascular endothelium, is dysfunctional in the aged vasculature. The glycocalyx has several functions that are critical for the maintenance of a healthy vasculature. We sought to determine if chronic dietary supplementation of glycocalyx precursors (glucosamine sulfate, fucoidan, superoxide dismutase, and high molecular weight hyaluronan) could restore glycocalyx function, while concomitantly ameliorating age‐related vascular dysfunction. Young (Y: 7 mo) and old (O: 30 mo) male B6D2F1 mice consumed a control (C) or glycocalyx precursor (GP: 37 mg/kg encapsulated chow provided courtesy of MicroVascular Health Solutions, LLC [U.S. Patent Serial No. 9,943,572]) diet ad libitum for 10 weeks. Glycocalyx barrier function (perfused boundary region [PBR]) was evaluated in the mesenteric microcirculation using an intravital microscope equipped with an automated capture and analysis system. PBR was ~13% higher in OC compared to YC, suggestive of an age‐related impairment in glycocalyx barrier function, and this was normalized in OGP mice (Both P<0.05; Figure 1). At baseline, aortic pulse wave velocity (PWV), a measure of large artery stiffness, was higher in OC and OGP compared with YC mice (Both P<0.05; Figure 2). However, after the dietary intervention, PWV decreased by ~13% in OGP (P<0.05), whereas, PWV was unchanged in OC and YC mice after the 10 week period (P>0.05). We assessed endothelial function by endothelium‐dependent dilation (EDD, maximal response to acetylcholine [ACh]) in the carotid artery. Carotid artery EDD was higher in YC and OGP compared to OC mice (92.5±2.4 and 90.7±2.3 vs. 69.0±4.9%, respectively, P<0.05). EDD of OGP was similar to YC mice (P>0.05). After incubation with the nitric oxide (NO) synthase inhibitor, L‐NAME, the dilatory response did not differ between groups (P>0.05). NO bioavailability (max ACh dilation ‐ max ACh+L‐NAME dilation) was ~10–14 fold higher in YC and OGP compared to OC mice (Both P<0.05; Figure 3). Endothelium‐independent dilation (vasodilation to sodium nitroprusside) was not different between groups (P>0.05). In young mice, GP diet did not affect any of the aforementioned measurements (P>0.05). In conclusion, 10 weeks of dietary GP supplementation in old mice restores glycocalyx barrier function that is accompanied by reduced aortic stiffness and augmented EDD and NO bioavailability, suggesting that the glycocalyx may be an effective therapeutic target for vascular dysfunction in older adults.
Video as Educational Multimedia to Teach English Speaking

The aim of this research is to develop an educational multimedia in the form of video as one of media to teach speaking. Research and development design is used to produce an educational multimedia entitled Nginggris Narsis. Procedures of this research includes identification students and teachers need, planning and product design, product small scale tryout, validation of the expert, product revision, product large scale try out and revision, and dissemination. This video is used to teach the first grade students of Junior High School in Indonesia of which curriculum implemented is 2013 curriculum. The results show that NginggrisNarsis is an appropriate material to teach EFL learners in speaking, although there are still some weaknesses found within this video. Nginggris Narsis as educational media to teach speaking help both teachers and students in introducing English as a foreign language for Indonesian students.
Midlife and older adulthood diabetes and hypertension may affect cognition later in life in more and less wealthy countries alike

We read the article by Muller et al. with great interest. [1] The limited possibilities for undertaking studies with brain scans in low and middle income countries (LMIC) also limits direct comparisons of the effect of diabetes and hypertension on cognition to the results in Iceland and the UK. [1,2] The most recent burden of disease data points to the high and increasing rates of diabetes and hypertension in LMIC. The prevalence of mild cognitive impairment in eight LMICs was similar to higher income countries. [3] In a pooled sample from China, Ghana, India, Mexico, the Russian Federation, and South Africa, prevalence of self-reported diabetes was 7.8% and hypertension was 29.2% in adults aged 50 years and older. [4] Measured hypertension resulted in a higher overall prevalence rate of 52.9%, from 32% in India to 78% in South Africa. [5] Awareness of having the condition was determined to be low in all the participating countries. The prevalence of having both conditions would likely result in even higher risk for cognitive impairment later in life. Considering the impacts on cognition and demographic aging occurring globally, disease prevention and treatment poses an issue in higher and lower income countries alike, with or without MRIs. 1. Muller M, Sigurdsson S, Kjartansson O, , et al. Joint effect of mid- and late-life blood pressure on the brain. The AGES- Reykjavik Study. Neurology 2014;82:2187-2195. 2. Roberts RO, Knopman DS, Przybelski SA, , et al. Association of type 2 diabetes with brain atrophy and cognitive impairment. Neurology 2014;82:1132-1141. 3. Sosa AL, Albanese E, Stephan BCM, et al. Prevalence, distribution, and impact of mild cognitive impairment in Latin America, China, and India: A 10/66 population-based study. PLoS Med2012: 9; e1001170. doi:10.1371/journal.pmed. 1001170. 4. He W, Muenchrath M, Kowal P, US Census Bureau. Shades of Gray: A Cross- \Country Study of Health and Well-Being of the Older Populations in SAGE Countries, 2007-2010. Washington DC: US Government Printing Office. 2012. 5. Lloyd-Sherlock P, Beard J, Minicuci N, Ebhrahim S, Chatterji S. Hypertension among older adults in low- and middle-income countries: prevalence, awareness and control. Int J Epidemiol 2014;1- 13:doi:10.1093/ije/dyt215. For disclsoures, please contact the editorial office at journal@neurology.org.
Neural Networks for the Web Services Classification

This article introduces a n-gram-based approach to automatic classification of Web services using a multilayer perceptron-type artificial neural network. Web services contain information that is useful for achieving a classification based on its functionality. The approach relies on word n-grams extracted from the web service description to determine its membership in a category. The experimentation carried out shows promising results, achieving a classification with a measure F=0.995 using unigrams (2-grams) of words (characteristics composed of a lexical unit) and a TF-IDF weight.
Isolation of bacteria from diesel contaminated soil for diesel remediation

This study is aimed at isolating bacterial species that inhabit diesel contaminated soil and also screened these isolates for the ability to be used for remediating diesel contaminated environment using their potential to degrade diesel as carbon and energy source. Top soil sample was collected from an ancient diesel-powered generator house in Minna, Nigeria, in a sterilized plastic container while diesel oil was obtained from local petrol bunk. Four bacterial isolates were isolated from the diesel contaminated soil sample and were screened for their ability to degrade diesel using mineral salt medium (MSM). The isolates with highest biodegradation potential were identified as Bacillus subtilis and Bacillus cereus. The optimum pH (5, 6, 7 and 8) and hydrocarbon concentration (1%, 2%, 5% and 10%) of the isolate was determined by spectrophotometry and the result revealed that the optimum pH for biodegradation of diesel by Bacillus subtilis and Bacillus cereus, was 7 (1.170) and 8 (1.745) respectively while the optimum hydrocarbon concentration degradation for both isolates was 5% (2.22) and 1% (2.37) respectively. The results of this study showed that these isolates were able to degrade diesel and can be useful for large scale bioremediation of diesel contaminated soils.
CRISPR-based bioengineering of the Transferrin Receptor revealed a role for Rab7 in the biosynthetic secretory pathway

The regulated secretory trafficking of neosynthesized transmembrane receptors is particularly challenging to investigate as it is under-represented at steady state compared to the abundance of the other trafficking routes. Here, we combined the retention using selective hook (RUSH) system to a CRISPR/Cas9 gene editing approach (eRUSH) to identify molecular players involved in the trafficking of neosynthesized Transferrin Receptor (TfR) en route to the plasma membrane (PM). TfR-eRUSH monoclonal cells expressing endogenous, ER-retainable and fluorescent TfR were engineered and characterized. Spatiotemporal quantitative proteomics of TfR-eRUSH cells allowed the identification of molecular partners associated with TfR-containing membranes and provided a comprehensive list of potential regulators, co-trafficking cargos, and enriched pathways. Furthermore, we chose to focus our attention on the Rab GTPase family members for their function as vesicle trafficking regulators and performed a Rab-targeted siRNA screen that we correlated to our proteomics data. Unexpectedly, we identified Rab7-harboring vesicles as an intermediate compartment of the Golgi-to-PM transport of the neosynthetic TfR. These vesicles did not exhibit degradative properties and were not associated to Rab6A-harboring vesicles, also involved in Golgi-to-PM transport. However, Rab6A-TfR vesicles delivered TfR directly to the PM, while in contrast, Rab7A was transiently associated to neosynthetic TfR-containing post-Golgi vesicles but dissociated before PM vesicle fusion. Together, our study proposes the eRUSH as a powerful tool to further study the secretory pathway and reveals an unforeseen role for Rab7 in the neosynthetic transport of the TfR, highlighting the diversity of the secretory vesicles’ nature for a given cargo.
Testes of DAZL null sheep lack spermatogonia and maintain normal somatic cells

Multiplying the germline would increase the number of offspring that can be produced from selected animals, accelerating genetic improvement for livestock breeding. This could be achieved by producing multiple chimaeric animals, each carrying a mix of donor and host germ cells in their gonads. However, such chimaeric germlines would produce offspring from both donor and host genotypes, limiting the rate of genetic improvement. To resolve this problem and produce chimaeras with absolute donor germline transmission, we have disrupted the RNA-binding protein DAZL and generated germ cell-deficient host animals. Using Cas9 mediated homology-directed repair (HDR), we introduced a DAZL loss-of-function mutation in male ovine fetal fibroblasts. Following manual single-cell isolation, 4/48 (8.3%) of donor cell strains were homozygously HDR-edited. Sequence-validated strains were used as nuclear donors for somatic cell cloning to generate three lambs, which died at birth. All DAZL-null male neonatal sheep lacked germ cells. Somatic cells within their testes were morphologically intact and expressed normal levels of somatic cell-specific marker genes, indicating that the germ cell niche remained intact. This extends the DAZL-mutant phenotype beyond mice into agriculturally relevant ruminants, providing a pathway for using absolute transmitters in rapid livestock improvement.
Sprue-Like Intestinal Disease Following Crohn’s Disease

A 48-yr old female was reviewed for ongoing symptoms of diarrhea and weight loss following a total colectomy for colitis. Pathological review of the surgical specimens revealed changes of Crohn’s colitis. Subsequent investigations after the colectomy revealed endoscopic evidence of duodenal mucosal scalloping, negative celiac serological studies and moderate to severe changes in villous architecture including crypt hyperplasia with complete villus atrophy and increased numbers of intra-epithelial lymphocytes. No changes were present in the distal small intestine. Over the course of almost a decade, measures directed towards symptom relief were effective. However, in spite of a strict gluten-free diet, repeated endoscopic biopsies of the proximal small bowel remained severely abnormal with persistent crypt hyperplastic villus atrophy. “Sprue-like” intestinal disease occurs in Crohn’s disease limited to the duodenum, mimicking biopsy changes of celiac disease and may represent a post-colectomy complication in Crohn’s disease.
Flemish Teachers’ Age-related Stereotypes: Investigating Generational Differences

ABSTRACT This quantitative study examines the existence of Flemish teachers’ age-related stereotypical beliefs and investigates differences between generational cohorts (N = 558). Results indicated that all generational cohorts studied attribute being interested in technological change, making use of ICT, having creative and innovative ideas, and considering another profession to younger teachers. At the same time, teachers in all cohorts perceive taking a leadership role, being loyal, being confident, being conservative, having smooth classroom management skills, and having extensive knowledge of the subject matter as characteristics of older teachers. Many characteristics, in terms of attitudes, are not clearly attributed to a certain age group of teachers.