Miracle-dz/UniFAR-FTUs huggingface dataset:
Peptide phage libraries can be an efficient tool for identifying antibody ligands for polyclonal antisera

We have examined the potential of isolating ligands for polyclonal antibodies from a nanopeptide phage library. The library was screened with a rabbit polyclonal antiserum raised against a synthetic peptide (ALWFRNHFVFGGGTKVT). Following screening, the positive phages were tested in an ELISA for their reactivity with the antiserum. Phages that showed positive reactivity with the antiserum compared with a normal rabbit serum were selected and their displayed peptides were determined. Among the 36 random positive clones, 31 clones carried the sequence AVFGGGTKL, PFFGGGSRA or APTGGSKRT that have a significant homology to the immunizing peptide. Five positive phages displayed the ATNIFIEGT sequence, which has no obvious linear homology with either the other selected peptides or with the peptide used for immunization. In contrast to the control peptide, the immunizing peptide inhibited binding of the antiserum to the peptide‐displaying phages in a dose‐dependent manner, thus demonstrating the specificity of the interaction. Furthermore, the rabbit B cell response to the peptide was found to be limited and focused on its C‐terminal. Taken together, our data demonstrate the potential of random peptide phage libraries for defining epitopes for polyclonal antisera as well as for investigation of the nature of B cell responses to any given antigen.
Probing the Association of Pancreatitis in Inflammatory Bowel Disease

Abstract:Inflammatory bowel disease (IBD) has been increasingly diagnosed in children and adults. Similarly, acute and chronic pancreatitis are increasingly prevalent conditions with potentially devastating consequences. There is a growing body of literature linking these 2 conditions. The purpose of this review is to provide a comprehensive outline of the association between IBD and pancreatitis and to explore their putative pathophysiology. Based on the collective reports, 2 outstanding reasons for pancreatitis in patients with IBD are medications and IBD complications.
Alphaviruses induce apoptosis in Bcl‐2‐overexpressing cells: evidence for a caspase‐mediated, proteolytic inactivation of Bcl‐2

Bcl‐2 oncogene expression plays a role in the establishment of persistent viral infection by blocking virus‐induced apoptosis. This might be achieved by preventing virus‐induced activation of caspase‐3, an IL‐1β‐converting enzyme (ICE)‐like cysteine protease that has been implicated in the death effector phase of apoptosis. Contrary to this model, we show that three cell types highly overexpressing functional Bcl‐2 displayed caspase‐3 activation and underwent apoptosis in response to infection with alphaviruses Semliki Forest and Sindbis as efficiently as vector control counterparts. In all three cell types, overexpressed 26 kDa Bcl‐2 was cleaved into a 23 kDa protein. Antibody epitope mapping revealed that cleavage occurred at one or two target sites for caspases within the amino acid region YEWD31↓AGD34↓A, removing the N‐terminal BH4 region known to be essential for the death‐protective activity of Bcl‐2. Preincubation of cells with the caspase inhibitor Z‐VAD prevented Bcl‐2 cleavage and partially restored the protective activity of Bcl‐2 against virus‐induced apoptosis. Moreover, a murine Bcl‐2 mutant having Asp31, Asp34 and Asp36 substituted by Glu was resistant to proteolytic cleavage and abrogated apoptosis following virus infection. These findings indicate that alphaviruses can trigger a caspase‐mediated inactivation of Bcl‐2 in order to evade the death protection imposed by this survival factor.
Réduction de modèle basée sur l'apprentissage par variété appliquée à l'optimisation du procédé d'emboutissage

Dans cette presentation, nous proposons de representer les toles embouties, apres retour elastique, par des fonctions distances. Nous determinons le nombre minimum de parametres necessaires afin de caracteriser la forme finale quel qu’en soit son degre de complexite, aboutissant a un “espace de formes” d’ordre reduit. Cette approche est ensuite utilisee dans un algorithme d’optimisation permettant de se rapprocher au mieux d’une forme cible donnee. Enfin, l’approche est appliquee sur un cas-test industriel d’emboutissage d’une tourelle d’amortisseur automobile.
Étude et réalisation de sources lasers fibrées impulsionnelles de forte puissance autour de 2 µm

Les travaux presentes dans ce manuscrit de these s'inscrivent dans l'etude et la realisation de sources lasers impulsionnelles a fibre autour de 2 µm. Le travail presente dans ce manuscrit se decline en deux parties. Une premiere partie est consacree a l'etude des sources lasers fibrees a commutation de gain emettant des impulsions d'une duree de quelques nanosecondes. Une source a ete realisee et caracterisee, elle genere des impulsions de 10 ns de duree, 16 µJ d'energie et 1,6 kW de puissance crete. Les impulsions sont ensuite amplifiees dans une fibre a large cœur pour atteindre plus de 150 µJ d'energie et 13 kW de puissance crete, l'ensemble de la source etant totalement fibre. La deuxieme partie est consacree a l'etude des sources a verrouillage de mode autour de 2 µm. Un oscillateur totalement fibre fonctionnant en regime solitonique a ete realise et caracterise, il genere des impulsions de 4,5 ps de duree et 62 pJ d'energie. Ces impulsions sont ensuite amplifiees afin d'atteindre plus de 8 kW de puissance crete et 30 nJ d'energie sans l'accumulation significative de nonlinearites. Les limites de l'amplification des impulsions dans ces deux differents regimes sont etudiees et discutees avec l'appui de modeles numeriques
Origins of the invasive red swamp crayfish (Procambarus clarkii) in the Santa Monica Mountains.

Although native to the southeastern United States, the red swamp crayfish ( Procambarus clarkii) has become established worldwide through accidental and intentional actions by humans. In the Santa Monica Mountains of southern California, the presence of the omnivorous crayfish is associated with the absence or reduced abundance of native amphibians. The original source of P. clarkii in southern California is unknown; however genetic analysis can be used to determine sources of invasion. We sequenced 16S rRNA subunit and cytochrome oxidase I (COI) mitochondrial genes to trace the origins of P. clarkii in the Santa Monica Mountains. The resulting haplotype network of the combined COI and 16S rRNA subunit genes showed 19 distinct haplotypes and suggested multiple introductions of crayfish to the Santa Monica Mountains from possible source locations in Texas, Florida and Louisiana. Identifying original sources and mechanisms of
Yesterday , Today and Tomorrow of Supercritical Fluid Extraction and Chromatography

The aim of this special issue of the American Journal of Analytical Chemistry (AJAC) on Supercritical Fluids is to promote knowledge about this rapidly developing area of analytical chemistry, which is very useful in fields such as the pharmaceutical and pesticide manufacturing industries, food production, clinical medicine and environmental studies. In this issue, the use of Supercritical Fluids (SFs) in Supercritical Fluid Extraction (SFE) and Supercritical Fluid Chromatography (SFC) is described.
Airway remodeling in asthma.

Asthma is estimated to affect 15 million people in the United States. After declining in the 1970s, its prevalence, morbidity, and mortality have increased since the 1980s. It now affects 1 in 7 children in the United Kingdom, and is the most frequent reason for preventable childhood hospitalizations in the United States. In 1990, total asthma-related health care costs were estimated at 6.2 billion dollars in the United States. This figure exceeded 10 billion dollars by 1995.
Ah receptor and estrogen receptor-dependent modulation of gene expression by extracts of diesel exhaust particles.

The ability of a methylene chloride extract of diesel exhaust particle (EDEP) to activate the aryl hydrocarbon receptor (AhR), bind to and activate the estrogen receptor (ER), and induce gene expression mediated via these nuclear receptors was examined in Hepa1c1c7 mouse hepatoma and MCF-7 human breast cancer cells. EDEP was able to induce a protein-DNA complex by gel retardation assays using a [gamma-32P]dATP-labeled dioxin response element (DRE). This complex could be effectively competed by a 150-fold excess of unlabeled DRE but not by a 150-fold excess of unlabeled mutated DRE. In Hepa1c1c7 cells that were transiently transfected with a DRE-regulated luciferase reporter gene, 4.6 ng/microliter EDEP treatment for 24 h resulted in a 22-fold induction of luciferase activity. In the same cell line, ethoxyresorufin-O-deethylase activity was significantly induced 20-fold following 24 h treatment with 4.6 ng/microliter EDEP. Using a competitive ligand binding assay, EDEP displaced bound tritiated E2 from the rat uterine ER in a dose-dependent manner with an IC50 of approximately 100 ng/microliter compared to the IC50 of E2, which was approximately 4.4x10(-4) ng/microliter (1.6 nM). In MCF-7 human breast cancer cells transiently transfected with a Gal4-regulated luciferase reporter gene (17m5-G-Luc) and a chimeric ER (Gal4-HEG0), treatment with 4.6 ng/microliter EDEP for 24 h resulted in a three-fold increase in luciferase activity (P<0.01) compared with the seven-fold increase observed with E2. This study demonstrates that EDEP is able to activate the AhR and ER and induce transcription of reporter genes regulated by these receptors' DNA response elements. Further study is required to identify the individual compound(s) responsible for the observed activity.
The afferent connexions of the nucleus of the horizontal limb of the diagonal band.

In the preceding paper (Price & Powell, 1970a) experimental evidence was presented to show that the centrifugal fibres of the olfactory bulb arise in the nucleus of the horizontal limb of the diagonal band, and in this paper the results of an investigation into the afferent connexions of this nucleus will be presented. From what is known of the organization of the central and centrifugal pathways in other sensory systems, it would be reasonable to predict that this part of the nucleus of the diagonal band receives fibres from one or more of the structures in which synaptic relays occur on the olfactory pathway. Such structures include, first, the olfactory bulb, secondly, those sites to which the bulb projects and finally, those areas which in turn receive third-order olfactory connexions (Powell, Cowan & Raisman, 1963, 1965; Cowan, Raisman & Powell, 1965). In the present study, therefore, lesions have been placed in most parts of the olfactory pathways and the resultant distribution of fibre and terminal degeneration examined in relation to the nucleus of the horizontal limb of the diagonal band (Price, 1969).
Advances in the diagnosis and treatment of ptosis

Purpose of review The surgical correction of blepharoptosis, both congenital and acquired, has been intensively examined and reported on for many years. This paper reviews recent publications on basic science, evaluation, technique modifications, and innovative materials in the care of ptosis patients. Recent findings The frontalis suspension technique is a commonly performed surgical correction of congenital blepharoptosis, used widely in the repair of ptosis with poor levator function. The repair typically includes using either tissue such as autologous or banked fascia lata or permanent suture material. The procedure involves connecting the motor unit (frontalis muscle) and the upper eyelid. Authors have recently reintroduced the technique of a dynamic frontalis muscle flap tunneled into the eyelid that directly attaches to the tarsal plate. Patients presenting with symptomatic blepharoptosis due to disinsertion or thinning of the levator aponeurosis require surgical repair. Multiple groups have tended toward a minimally invasive approach directed specifically at the levator aponeurotic defect. Proposed advantages of a small eyelid incision (8-13 mm) include less local anesthetic and tissue distortion, less ecchymosis and edema, decreased operative times, a shortened recovery period, and improved surgical results. Summary Surgical correction of congenital blepharoptosis may be performed with autologous fascia lata, cadaveric allograft, or permanent suture material. The use of a frontalis muscle advancement flap is elegantly designed; however, its role in clinical practice remains to be defined. Advancement of the levator aponeurosis for senile blepharoptosis may be preformed via a minimally invasive small incision approach. Patients benefit with decreased operative time, edema, ecchymosis, and recovery times.
Calibration sur modèle CAO pour la mesure par vision in-situ

Dans le cadre de l'amelioration de procede de fabrication, les travaux presentes concernent une methode de mesure in-situ par moyen optique. L'objectif est de mesurer les defauts lies au procede de fabrication, dans un temps reduit, pour finalement proposer des actions correctives. Ainsi une grande interoperabilite entre les donnees FAO et la mesure est necessaire. Elle peut etre realisee par la mise au point d'une methode de calibration permettant de realiser les mesures directement dans le repere utilise pour la construction de la maquette numerique et le calcul des trajectoires d'usinage. Nous proposons de realiser cette calibration en nous appuyant sur des entites presentes dans la maquette numerique et facilement identifiable dans l'image prise par le systeme de vision. Apres avoir rappele le principe de la calibration d'une camera et les differentes methodes usuellement utilisees pour la determiner, nous presentons une nouvelle methode basee sur l'association de diverses entites (droites, points) detectees dans l'image. Une application sur un exemple est ensuite proposee et permet de montrer l'efficience de la methode et l'importance de l'identification des elements utilises pour la calibration. Enfin une etude de sensibilite est donc menee en considerant des incertitudes associees aux positions et orientations des elements extraits de l'image.
Relationship of mood disturbance to cigarette smoking status among 252 patients with a current mood disorder.

BACKGROUND The relationship between cigarette smoking and mood has received increasing attention. This retrospective study evaluated the relationship between mood disturbance and cigarette smoking status among patients with a current mood disorder. The association between level of nicotine dependence and severity of mood disturbance was also evaluated among current smokers. METHOD Retrospective data for 252 patients (63.5% male, 85.0% white) admitted for treatment of a mood disorder at the San Diego Veteran Affairs Mental Health Clinical Research Center between November 1988 and June 1997 were studied. All current cigarette smokers at admission (N = 126) were matched with nonsmokers (N = 126) on the primary DSM-IV Axis I mood disorder diagnosis, admission status (inpatient or outpatient), gender, age (+/- 5 years), and ethnicity. The Hamilton Rating Scale for Depression (HAM-D), the Beck Depression Inventory, and the Profile of Mood States (POMS) were administered to patients on admission. Conditional logistic regression analysis for matched sets with a backward elimination was used to identify factors independently predictive of current smoking status. RESULTS A greater number of cups of coffee consumed per day (p = .002), a history of alcoholism (p = .004), and higher POMS fatigue subscale scores (p = .007) were predictive of current smoking status. Among current smokers, the HAM-D terminal insomnia item was positively associated with mean number of cigarettes smoked per day (p = .012). CONCLUSION Cigarette smoking should be addressed in the treatment of patients with a current mood disorder. Smokers experience greater levels of fatigue than nonsmokers. In addition, higher cigarette consumption levels are associated with mild-to-severe symptoms of terminal insomnia.
Dwarfism with retinal atrophy and deafness

The two children with this dystrophy, a girl aged seven years and eleven months and a boy aged six years and three months were admitted to the Hospital for Sick Children, Great Ormond Street, in June, 1935. The parents, who are natives of north Hampshire, are of English race, normal and not blood-relations, and they have been unable to trace the occurrence of the condition in their ascendants or collaterals. Dr. N. F. Kendall, under whose care they were before admission, has ascertained that before marriage the parents lived sixteen miles apart, and as their families have lived in the neighbourhood for a long time it is not unlikely that there has been intermarriage between them some generations back. There are four older children, three girls, aged eighteen, fifteen, and twelve years respectively, and a boy, aged ten, and one younger, a boy aged three years, who is taller than either of the affected ones. The dwarfs are so much alike in facial appearance, build and disposition, that the same general description will suffice (fig. 1, 2). Both have small heads, that of the girl being the smaller (fig. 3), but, although the vault of the skull is flattened and the circumference small, the general shape is normal, and neither child has the receding forehead characteristic of microcephaly. Their faces are small with sunken eyes and prominent superior maxillae. They are slightly built with short, slender trunks and unduly long legs, and their feet and hands are too large in proportion. The third and fourth fingers of their hands are deviated a little towards the mesial line.
MODELING OF POLLUTION DISPERSION AROUND A CUBIC OBSTACLE

A code has been developed in C++ to solve the Navier-Stokes, continuity, and mass transfer equations using large eddy simulation (LES) for the turbulent flow. The pollution dispersion around a cube was investigated. The pollutant source was placed on the ground, right behind the cube. In the code, the finite volume (FV) formulation applied on the staggered grid arrangement for a structured mesh system. Smagorinsky-Lilly sub-grid scale (SGS) model was used. The discretisation scheme for all terms except the convection terms in the momentum equations, which adopts a TVD scheme, was the central difference scheme. The PISO algorithm showed faster convergence behavior than the other well known algorithm such as SIMPLE. The pollution dispersion behind the cube has been obtained assuming that the molecular diffusivity is dominant and neglecting the buoyancy effects. Jalal Abedi and Mohammad Omidyeganeh 2
Pattern of Clubfoot Deformity and Adherence to Ponseti Treatment among Children with Clubfoot Deformity

Background: Clubfoot is painless in a baby, but it can eventually cause discomfort and become a noticeable disability if left untreated. These symptoms become more obvious and more of a problem as the child grows. Objective: This study was conducted to assess pattern of clubfoot deformity and adherence to ponseti treatment among children with clubfoot deformity. Methods: It was a cross sectional descriptive study. A total of 143 club foot children, age between 1 day to 5 years of both male and female were selected purposively to conduct this study. Face-to face interview method was adopted by using semi-structured questionnaire. Results: Most of the children were boys. More than one third (38.5%) children were in age group 13 to 36 months. Study found about 63 (44%) children had both feet deformity, 49 (34.3%) children had right foot deformity and rest of 31 (21.7%) children had left foot deformity. More than half (53.8%) of the children had typical flexible clubfoot deformity. The second highest (25.9%) children had typical rigid deformity and rest of 12.6% and 7.7% had atypical rigid and atypical flexible clubfoot deformity respectively. About 67.1% (96) children were needed bracing followed by plaster casting 32.2% (46) and 0.7% (1) was given home advice. Majority (88.8%) of the respondents reported that they have been taking treatment regularly where 11.2% (16) reported that they were irregular in taking treatment. Conclusion: This study presents a patient with Congenital Talipes Equino Varus successfully managed by Physical Therapy approach. Finally, there is need to decentralize clubfoot treatment services away from referral hospitals to the people in the community through outreach programs.
HEMATOLOGY OF THE NORMAL MONKEY *

Several years ago the Radiobiological Laboratory of the University of Texas and the United States Air Force started an extensive research program using the Indian Macaca mulatta monkey. The need for reliable information concerning the hematology of the normal monkey led to a search of the available literature. As shown in this table, many investigators have been interested in the macaque; however, few of these investigators worked with large numbers of animals or made large numbers of observations on a small number of animals. In many case5 no reference was made to the number of animals or the number of observations leading to the cited results. In order that some more useful information concerning the hematology of the normal monkey might be obtained, a series of observations on many of the normal animals serving as controls for various experimental procedures was begun in 1953 and continued for several years.
The Role of Holistic Processing in Judgments of Facial Attractiveness

Previous work has demonstrated that facial identity recognition, expression recognition, gender categorisation, and race categorisation rely on a holistic representation. Here we examine whether a holistic representation is also used for judgments of facial attractiveness. Like past studies, we used the composite paradigm to assess holistic processing (Young et al 1987, Perception 16 747–759). Experiment 1 showed that top halves of upright faces are judged to be more attractive when aligned with an attractive bottom half than when aligned with an unattractive bottom half. To assess whether this effect resulted from holistic processing or more general effects, we examined the impact of the attractive and unattractive bottom halves when upright halves were misaligned and when aligned and misaligned halves were presented upside-down. The bottom halves had no effect in either condition. These results demonstrate that the perceptual processes underlying upright facial-attractiveness judgments represent the face holistically. Our findings with attractiveness judgments and previous demonstrations involving other aspects of face processing suggest that a common holistic representation is used for most types of face processing.
Réalisation d'un Brain-Switch EEG par Géométrie Riemannienne

Le domaine des interfaces cerveau-machine represente un reel espoir pour des personnes handicapees moteur afin de commander un effecteur a travers la modulation de leur activite cerebrale. En vue d'une application dans un contexte de vie courante, il est necessaire de mettre au point un systeme asynchrone robuste de detection d'une activite mentale specifique a partir des mesures EEG. La realisation d'un tel Brain-Switch est abordee dans ce papier a travers l'utilisation des outils de la geometrie Riemannienne pour manipuler et classer les matrices de covariance spatiale, choisies comme descripteur de l'activite mentale sous-jacente. La methode est testee sur des acquisitions EEG realisees au sein du laboratoire. Les resultats demontrent une bonne qualite de detection avec un pourcentage de declenchement volontaire de l'ordre de 92% et de declenchements reussis de l'ordre de 91%.