Miracle-dz/UniFAR-FTUs huggingface dataset:
Engineered T Cell Receptor-Mimic Antibody, (TCRm) Chimeric Antigen Receptor (CAR) T Cells Against the Intracellular Protein Wilms Tumor-1 (WT1) for Treatment of Hematologic and Solid Cancers

Adoptive transfer therapy of T cells expressing chimeric antigen receptors (CAR) against tumor-associated antigens has been shown to be clinically successful in a limited set of leukemia. However, novel antigen targets for both hematological and solid malignancies are required. Most CARs described thus far are targeted against external antigens on particular cell types. We have designed and engineered the first CAR T cell against a human intracellular protein, WT1. WT1 is overexpressed in many cancers, including acute and chronic leukemias and numerous solid tumors. Our TCRm CAR, derived from the ESK1 TCRm mAb, termed WT1 28z, is reactive with the RMFPNAPYL peptide of the WT1 protein that is processed and presented on the surface of cells in the context of HLA-A*02:01. WT1 28z expressing T cells have high expression of the CAR on their surface. They are cytotoxic in standard 51 Cr assays against a range of cancer cell lines, including the megakaryoblastic cell line SET2, the acute myeloid leukemia (AML) cell line AML14, the multiple myeloma cell line KARPAS, and the ovarian cancer line, OVCAR3, as compared to CAR T cells against an irrelevant antigen. The WT1 28z CAR T cells are also cytotoxic against primary AML bone marrow blasts in this assay. When co-cultured with these primary cells or cancer cell lines, the WT1 28z CAR T cells have enhanced production of proinflammatory cytokines such as IFN-g, IL-2, and GM-CSF, as compared to irrelevant CAR T cells. Importantly, WT1 28z T cells are specific for the WT1-HLA-A*02:01 complex. The cells do not show cytotoxicity against cell lines or primary cells that are not both HLA-A*02:01- positive and WT1 positive. WT1 28z T cells are currently being tested alongside irrelevant antigen CAR T cells in AML and ovarian cancer murine models in vivo to assess efficacy, with the ultimate goal of translating this novel approach into the clinical setting for both hematological and solid cancers. The data provide the proof-of-concept that CAR T cells also may be directed at intracellular antigens. Disclosures Dao: Novartis: Patents & Royalties. Liu: Eureka: Employment, Inventor Other. Scheinberg: Novartis: Patents & Royalties. Brentjens: Juno Therapeutics: Consultancy, Scientific co-founder and Stock holder Other.
The Trust-Building Nature of Identity Verification in the Sharing Economy: An Online Experiment

Despite being widely used in practice and often asserted to be an effective trust-building mechanism, little empirical evidence exists regarding the effect of identity verification on sharing economy platforms. We theoretically develop a model based on signaling theory to explain how identity verification strengthens users ’ intention to engage in sharing economy transactions by increasing the level of trust towards a verified transaction partner. To test our hypotheses, we design a between-subject online experiment comparing subjects ’ perceptions of identity-verified and non-verified user profiles on an accommodation sharing platform. Data was collected from 232 participants and analyzed using covariance-based structural equation modeling. We found identity verification to significantly increase transaction intention, while its effect was mediated by trust in the transaction partner. Moreover, trust disposition was found to be a significant antecedent of users ’ transaction intentions. We discuss our findings and provide implications for theory and practice.
Contribution to the bryophyte flora of India: Agasthyamalai biosphere reserve in Western Ghats.

The bryophyte flora of the Agasthyamalai Biosphere Reserve is catalogued. There is no previous report of bryophytes from this area. The checklist consists of 90 taxa (58 mosses, 32 liverworts), of which 16 species are newly reported for the Peninsular India (Asterella reticulata, Bazzania sumbavensis, Cephalozia pandei, Clastobryopsis muelleri, Cyathophorella adiantum, Dicranoloma subreflexifolium, Herbertus dicranus, Himantocladium cyclophyllum, Hypnum plumaeforme, H. sikkimense, Leucobryum cucullifolium, Radula grandifolia, Symblepharis vaginata, Symphyodon echinatus, Trichocolea udarii and Trichostelium boschii) and another 6 are new for the Kerala State (Campylopus involutus, Cephaloziella willisiana, Frullania ericoides, Macromitrium moorcroftii, Metzgeria decipiens and Leucobryum mittenii).
Effect of the Combined Intervention with Passive Whole-Body Vibration and Auriculotherapy on the Quality of Life of Individuals with Knee Osteoarthritis Assessed by the WHOQOL-Bref: A Multi-Arm Clinical Trial

The aim of this study was to determine the effect on the quality of life of two non-pharmacological interventions isolated or in combination: (i) passive whole-body vibration exercise (WBVE), and (ii) auriculotherapy (AT). One hundred three participants with knee osteoarthritis (KOA) were allocated to: (a) a vibration group (WBVEG; n = 17) that performed WBVE (peak-to-peak displacement: 2.5 to 7.5 mm, frequency: 5 to 14 Hz, Peak acceleration: 0.12 to 2.95 g), two days/weekly for five weeks, (b) an AT group (ATG; n = 21), stimulation of three specific auriculotherapy points (Kidney, Knee and Shenmen) in each ear pavilion, (c) WBVE + AT (WBVE + AT; n = 20) and (d) respective control groups (WBVE_CG, n = 15; AT_CG, n = 12; WBVE + AT_CG, n = 18). The participants filled out the WHOQOL-bref Questionnaire before the first and after the last sessions. Statistical differences in the various domains of the WHOQOL-bref were not found. In conclusion, WBVE or AT alone or combined did not contribute in altering the quality of life of individuals exposed to these interventions.
Bioavailability of avocado‐derived mannoheptulose in dogs

Mannoheptulose (MH) is a 7‐carbon sugar found in avocados and other natural sources that acts to reduce glycolysis via hexokinase inhibition. It has been proposed as a calorie restriction (CR) mimetic that delivers anti‐aging and health‐promoting benefits of CR without reducing food intake. Three studies were conducted to evaluate MH bioavailability when fed to dogs as an avocado extract (AvX) based on MH levels in urine (Study 1) and plasma (Study 2 & 3). In Study 1, Labrador Retrievers (LR; n=15) and Fox Terriers (n=15) were fed AvX‐containing diets formulated to deliver 0, 2 or 5 mg MH/kg BW. All dogs were subjected to 24‐hour quantitative urine collections. A dose‐dependent increase (p<0.05) in urinary MH occurred with increasing dietary MH. In Study 2, LR (n=6) were fed AvX‐containing diets once daily to deliver 0, 1 or 2 mg MH/kg BW. Sequential blood samples were collected before and after feeding through 12 hr and at 24‐hr post‐feeding. Plasma MH increased (P<0.05) with both MH diets compared to control. Peak MH occurred 6–8 hr post‐feeding and returned to non‐detectable levels by 24 hr. In Study 3, similar MH results were observed for LR (n=10) fed AvX‐containing diets twice daily to provide 0 or 2 mg MH/kg BW. Peak MH occurred within 2–4 hr of MH consumption and returned to non‐detectable levels by 24 hr. Mannoheptulose, fed as an avocado extract, is biologically available in dogs based on its appearance in plasma and urine.
Dynamics of a metapopulation epidemic model with localized culling

A two–patches metapopulation mathematical model, describing the dynamics of Susceptibles and Infected in wildlife diseases, is presented. The two patches are identical in absence of control, and culling activities are performed in only one of them. Firstly, the dynamics of the system in absence of control is investigated. Then, two types of localized culling strategies (proactive end reactive) are considered. The proactive control is modeled by a constant culling effort, and for the ensuing model the disease free equilibrium is characterized and existence of the endemic equilibrium is discussed in terms of a suitable control reproduction number. The localized reactive control is modeled by a piecewise constant culling effort function, that introduces an extra–mortality when the number of infected individuals in the patch overcomes a given threshold. The reactive control is then analytically and numerically investigated in the frame of Filippov systems. We find that localized culling may be ineffective in controlling diseases in wild populations when the infection affects host fecundity in addition to host mortality, even leading to unexpected increases in the number of infected individuals in the nearby areas.
„Liquid biopsy“: Analyse molekularer Marker aus Blutproben zur Therapiesteuerung beim Lungenkarzinom

Zusammenfassung Zielgerichtete Therapien haben erfolgreich Einzug in die palliative Therapie des Lungenkarzinoms gehalten, da sie eine Individualisierung oder Personalisierung ermöglichen, die mit der Testung von prädiktiven Biomarkern einhergeht. Dadurch steigt die Zahl an prädiktiven molekularbiologischen und immunhistochemischen Untersuchungsmöglichkeiten zu verschiedenen Zeitpunkten der Therapie und damit der Bedarf an jeweils aktuellen Tumorgewebeproben. Diese sind jedoch oft nicht ohne unverhältnismäßig hohe Belastungen für den Patienten zu gewinnen. Daher stellen Verfahren der Diagnostik aus Blutproben, die mit dem aus dem Englischen stammenden Begriff „liquid biopsy“ zusammengefasst werden, eine Alternative bzw. Ergänzung zur klassischen Gewebebiopsie dar. Derzeit können in der klinischen Routine bereits aktivierende EGFR-Mutationen sowie die inhibitorische Mutation T790 M aus dem Blut detektiert werden. Dieser Artikel stellt den aktuellen Stellenwert der liquid biopsy in der Diagnostik, zur Prognose und zur Steuerung des Therapieverlaufs bei Lungenkarzinomen dar und gibt einen Ausblick auf zukünftige Möglichkeiten.
What parties want from their leaders: How office achievement trumps electoral performance as a driver of party leader survival

Rational choice theories of political behaviour start from the premise that parties seek policy, office, and votes. In accordance with this premise, previous research has shown that electoral performance and office achievement independently affect party leader survival. However, we know little about how goal attainment interacts across these two domains. This paper proposes a novel hypothesis stating that intrinsic goals (office) dominate over purely instrumental ones (votes). As a result, the impact of electoral performance on party leader survival should be conditional on office achievement. Using data on over 500 party leaders in 14 parliamentary democracies between 1965 and 2012, we show that electoral performance and office achievement strongly affect leadership turnover. However, we also demonstrate that the electoral performance effect disappears when parties enter or exit office at the same time. These results constitute the best direct evidence to date that parties prioritise office achievement over electoral success.
Buying Unethical Loyalty: A Behavioral Paradigm and Empirical Test

Unethical behavior is often accompanied by others covering up a transgressor’s actions. We devised a novel behavioral paradigm, the Unethical Loyalty Game (ULG), to study individuals’ willingness to lie to cover up others’ dishonesty. Specifically, we examined (i) whether and to what extent individuals are willing to lie to cover up others’ unethical behavior, (ii) whether this unethical loyalty depends on the benefits (bribe) at stake, and (iii) whether trait Honesty–Humility accounts for interindividual variability in unethical loyalty. In a fully incentivized experiment (N = 288), we found a high prevalence of lying to cover up others’ unethical behavior, which increased with increasing bribes. In turn, unethical loyalty decreased with individuals’ Honesty–Humility levels. Overall, the findings show that most but not all individuals are corruptible to disguise others’ transgressions. Future research using the ULG can help to further illuminate (the determinants of) this prevalent type of unethical behavior.
Relation between the Total Diet Quality based on Korean Healthy Eating Index and the Incidence of Metabolic Syndrome Constituents and Metabolic Syndrome among a Prospective Cohort of Korean Adults

Methods: Based on a community-based cohort of the Korean Genome and Epidemiology Study (KoGES) from 2001 to 2014, data from a total of 5,549 subjects (2,805 men & 2,744 women) aged 40~69 years at the baseline with a total follow-up period of 38,166 personyears were analyzed. The criteria of the National Cholesterol Education Program Adult Treatment Panel was employed to define metabolic syndrome. The total diet quality was estimated using the Korean Healthy Eating Index (KHEI). Hazard ratios (HR) and 95% confidence intervals (CI) for risk of metabolic syndrome constituents and metabolic syndrome in relation to KHEI quintile groups was calculated by multivariate Cox proportional hazards regression model.
Interorgan influences in seedling growth of chickpea revealed through selective organectomy

Selective organectomy performed on 7 day old seedlings of Cicer arietinum L. with persistent cotyledons resulted in the display of several correlative growth responses. Derooting, partial or complete, did not influence seedling growth. Decapitation or partial deshooting resulted in the loss of apical dominance with the growth of a branch from the topmost available axillary bud only, whereas complete deshooting caused the development of cotyledonary axillary branches. Intriguingly, single decotyledonation in such seedlings resulted in almost a complete suppression of the bud growth in the axil of remaining intact cotyledon. The relative significance of retained cotyledons with mobilizable reserves vis a vis photosynthesizing leaves, for the seedling growth may be inferred to some extent by the fact that whereas the seedling growth was significantly reduced by decotyledonation, being proportional to the number of cotyledons excised, a partial or even complete defoliation had no effect on branch growth. To achieve brevity, clarity and precision of description of the status of modifications of seedlings made through selective organectomy, a Seedling Configuration Formula (SCF) has been proposed.
Why are there proteins in the urine of healthy people?

Proteins are essential nutrients for humans, but why is there a small amount of proteins excreted through urine? Although healthy people have low levels of protein in the urine, they are rich in variety. The proteins in them may be unnecessary wastes of the body or may even have a toxic effect on the body and must be excreted. Compared with small-molecule metabolites, proteins have a complex structure and various functions. Even a small change at the molecular level will affect their subsequent biological functions. A comprehensive comparison of the molecular level modifications of plasma and urine proteome, the difference may provide clues to the presence of protein in the urine. In this study, a total of 9 healthy people’s urine and 9 healthy people’s plasma samples were collected. The samples were analyzed by high-resolution tandem mass spectrometry and unlabeled quantitative proteomics techniques, and the non-limiting modification identification algorithm was used to to compare molecular modifications between two types of samples as a whole. The results showed that the amount of cysteine (Cys) modification to dehydroalanine (Dha) in the urine proteome was higher than that in plasma. The molecular modification [Cys→Dha] destroys the disulfide bond of the original protein, thereby affecting or even changing the structure and biological function of the protein. Therefore, this study revealed the differences in the overall proteome modification of plasma and urine and pointed out that the structure of protein may be irreversibly changed due to the difference in modification of protein molecules, which in turn caused proteins which lost their functions and even toxic proteins from the blood to be excreted into the urine.
The influence of lead on the growth and development of various mustard types

The article studies the capacity of two types of mustard to accumulate Pb from contaminated sierozem soils. The dependence between the degree of lead contamination of soil (from 150 to 550 mg/kg) and the intensity of its entry in the vegetative and generative organs of brown (Brassica Juncea L.) and white mustard (Sinapis Alba L.) have been thoroughly examined. It has been established that with an increase of Pb content in soil, there is increased content of this metal in organs of the above plants. The effect of vermicompost, sulfur- and perlite-containing waste and their mixtures, the lead behavior in the system of sierozem soil and mustard plants were studied. In conducted experiments, the following soil mass ratios were used: sulfur- and perlite-containing waste:vermicompost = 3:1:0.5. The introduction of vermicompost or its mixture with the sulfur- and perlite-containing waste into the contaminated soil helps to reduce the concentration of Pb, which passes into various plant organs. At the same time, the lead content in mustard organs decreases in the following order: the roots > leaves > stalks > grains. It was revealed that, despite the increased lead content in the soil (from 150 to 550 mg/kg of soil), its translocation amount in the grain portion of mustard does not exceed the standard maximum permissible concentration value for food products (MPC = 0.5 mg / kg).
Role of non-conventional chemicals in biochemical defence against charcoal rot of sesame incited by Macrophomina phaseolina (Tassi) Goid

Charcoal rot of sesame (Sesamum indicum) incited by Macrophomina phaseolina (Tassi) Goid. is an economically important disease in India and worldwide. Seed and soil borne nature, non-specific, wide host range of pathogen, difficult to manage with fungicides and absence of complete genetic resistance impelled to biochemical defence analysis against this pathogen. Treatment with non-conventional chemicals induces defence against pathogen in the form biochemical changes in plant. Hence, the present investigation was carried out to know the impact of non-conventional chemicals on changes in defence biochemical parameters in plant. As the concentration of different non conventional chemicals increased from 50, 100 and 200 ppm there was reduction in incidence of charcoal rot disease. Maximum induction of resistance was recorded by the application of salicylic acid at 200 ppm concentration after challenge inoculation with M. phaseolina followed by indole acetic acid, indole butyric acid, acetyl salicylic acid, riboflavin and thiamine. The activity of total phenols and sugar content was reached at peak after six days of challenge inoculation with the pathogen and subsequently activity of these parameters slightly declined after six days of inoculation in all tested non-conventional chemicals. At each concentration, SA showed maximum induction of total phenol and total sugar followed by IAA, IBA, ASA, thiamine and riboflavin. However, sesame variety HT 2 showed higher induction of biochemical activities and lower disease incidence of charcoal rot as compared to HT 1.
The 3D Distribution of Long-period Mira Variables in the Galactic Disk

Long-period Mira variable stars are considered to have relatively high initial masses and may be potentially useful as tracers of spiral arm structure of the Milky Way. From 2004 to 2017, we monitored long-period Mira candidates selected from the IRAS color–color diagram in the near-infrared K′ band. As an initial result of this study, we found 108 Mira variables and determined their periods, mean magnitudes, and amplitudes. Most of them are located between 0° and 90° in Galactic longitude. The peak of their period distribution is at around 500 days, which is longer than the typical value for Mira variables selected in optical surveys. Distances to our Mira variables have also been estimated using the period–luminosity relation (PLR) in 3.4 μm with the help of a three-dimensional map of interstellar extinction. While the Ks-band PLR has a large scatter at longer periods (log P > 2.6), the PLR based on the Wide-field Infrared Survey Explorer 3.4 μm data has a much smaller scatter. We compare the spatial distribution of our sample to the spiral arms in the literature, and discuss the possible association of the long-period Mira variables with the spiral arms although the limited spatial coverage and the limited distance accuracy of the current sample prevent us from drawing a firm conclusion.
The Role and Influence of “Clip Thinking” on the Educational Process in Higher Medical Institutions

Information technology has strengthened the role of information, leading to the fact that modern society has reached a new stage of development – information. A fundamentally new way of communication and information broadcasting called the “screen culture” is emerging. The information society influences the society of people, their training and management processes. This influence is transforming our thinking, and therefore such concepts as “human-screen”, “clip thinking” and “clip consciousness” appear more often in the academic literature. In connection with the informatization of education, there are risks of losing the creative culture-generating ability of entire generations. If the older generation still retains other forms of perceiving the information provided (comprehension, comparison, analysis, criticism, etc.), the younger generation, especially students, are rather inclined towards the dynamic and mosaic “clip”, and receiving, processing and generating visual information, primarily educational.
Tensile Strength

In studies of contemporary Chinese art, Lin Tianmiao’s work has been overwhelmingly discussed in terms of women’s crafts and maternal roles. Citing her use of embroidery and the female figure, these interpretations have led to broad and often simplified characterizations of her work as “women’s art.” In focusing exclusively on symbolic allusions to gender representation, these discussions overlook the possibility of more complex narratives arising from Lin’s artistic concerns. By starting from the formal, material, and spatial components of her work, this article reveals how Lin has enacted penetrating investigations into manifestations of resistance and tension between physical forms and objects. Replete with taut lines and trembling vibrations, her work scrutinizes the nature of her materials, their limitations, and relationships among different parts of an installation. By tracing such formal and spatial devices, this article reveals three central topics at the heart of Lin’s oeuvre: the insufficiency of language, the urgency of form, and latent visibility. The exploration of these abstract concepts helps us move away from overt symbolic readings of her materials. These topics help show how Lin uses her art as tactics of intervention for interrogating practices of classification in contemporary Chinese art. As such, this article does not discount commentary on identity or gender but, rather, allows for richer, interrelated frameworks for understanding both her work and how it has been historically treated.