Miracle-dz/UniFAR-FTUs huggingface dataset:
Primate evolution of the alpha-globin gene cluster and its Alu-like repeats.

The arrangement of alpha-globin genes in Old World and New World monkeys and a prosimian, galago, has been determined by restriction mapping. Recombinant DNAs containing galago and Old World monkey alpha-globin genes have been isolated and subjected to a partial sequence determination for comparison to alpha-globin genes in human, chimpanzee and non-primate mammals. The results of this extensive structural analysis are relevant to several topics concerning the evolution of primate alpha-globin genes and Alu family repeats. All orders of higher primates (i.e. Old and New World monkeys, chimpanzee and human) have the same arrangement of alpha-globin genes. In contrast, the arrangement and correction of galago alpha-globin genes differ from those of higher primates, but are similar to those of non-primate mammals. The 5' and 3'-flanking regions of the human alpha 1 gene are orthologous to the corresponding region in galago, identifying the human alpha 2 gene as the more recently duplicated gene. The human psi alpha 1 gene is found to be inactivated after divergence of the human and galago lineages but prior to the divergence of human and monkey. Orthologous Alu family members in human and monkey DNAs indicate that the dispersion of some Alu repeats occurred prior to the divergence of these lineages. However, the Alu-like repeats of prosimian and higher primates result from entirely independent events giving rise to different repeat elements inserted at distinct genomic positions.
Increase in reciprocal Ia inhibition during antagonist contraction in the human leg: a study of motor units and the H reflex.

1. The change in reciprocal Ia inhibition of soleus motoneurones produced by stimulation of the common peroneal nerve was investigated by the use of twenty‐three soleus motor units as well as the soleus H reflex in six normal subjects during tonic pretibial contraction. 2. In the motor unit experiments, motoneuronal excitability was measured as the ‘critical firing stimulus’ (CFS), which is the difference between the test stimulus intensity needed to reach the threshold for the lowest threshold Ia fibres and the intensity which evokes firing of a motor unit with the probability of 50%. The conditioning effect, assessed from the change in the CFS, was expressed as a percentage of the unconditioned CFS. 3. At a conditioning intensity of 0.95 times the motor threshold value, there was Ia inhibition in sixteen of the twenty‐three motor units (69.6%) at rest. Of these sixteen motor units, twelve showed increases in inhibition at intervals below 2.0 ms during pretibial contraction. In four of the remaining seven units, inhibition first appeared during contraction. There was no significant decrease in inhibition at any time during contraction. 4. Based on the conventional H reflex, reciprocal Ia inhibition increased during very weak (below 2% of the maximum) voluntary dorsiflexion and continued to increase at a slightly stronger (3‐8% of the maximum) contraction, then decreased continuously when contraction was strengthened further. Maximal inhibition occurred at a relatively strong contraction when a weak conditioning stimulus was used, and vice versa. 5. We conclude that the activity of reciprocal Ia inhibitory interneurones increases during tonic antagonist contraction. The previous controversy about this inhibition is the result of occlusion at the Ia interneuronal level.
Mitochondrial DNA variation in rainbow trout (Oncorhynchus mykiss) across its native range: testing biogeographical hypotheses and their relevance to conservation

North‐western North America has been repeatedly glaciated over most of the past two million years, with the most recent glaciation occurring between 60 000 and 10 000 years ago. Intraspecific genetic variation in many species has been shaped by where they survived glaciation and what postglacial recolonization routes were used. In this study, molecular techniques were used to investigate biogeographical, taxonomic and conservation issues in rainbow trout, Oncorhynchus mykiss. Mitochondrial DNA (mtDNA) variation was assessed using a restriction fragment length polymorphism (RFLP) analysis, focusing mainly on the previously understudied northern extent of the species’ range. Two phylogenetically distinct mitochondrial lineages were found that differed from each other by up to 1.8% in sequence. Although the geographical distributions of the two clades overlap extensively, diversity and distributional analyses strongly suggest that trout survived glaciation in both coastal and inland refugia followed by postglacial gene flow and secondary contact. Postglacial dispersal into British Columbia most likely occurred from the Queen Charlotte Islands and the Columbia River. Although trout most likely also survived glaciation along the coast of Washington, Oregon and California, as well as near the Bering Strait, evidence suggests that dispersal into British Columbia from these areas was limited. Sequence analysis of mitochondrial haplotypes revealed higher diversity in California than in the northern part of the species’ range, indicating an ancient presence of the species in the south. Phylogeographic divergence probably predates adaptive variation in the species as suggested by evidence for parallel evolution of life history types across the range of O. mykiss.
The Changing Face of Women Managers in Malaysia and Singapore

First, Malaysia. In Malaysia the government has recently implemented economic plans that emphasize building an equitable society, developing a knowledge-based economy, and focusing on HR development. On the legal front, legislative amendments have been implemented to promote greater participation of women in the labour market. These changes should provide increased opportunities for Malaysian women. However, due to the plurality of the Malaysian society there may be differences in terms of social and cultural expectations of women from different ethnic groups, which may work against women’s advancement in the workplace. Omar (2009) in her study argues that altering the social attitudes towards working women may be a greater challenge for Malaysia. The chapter examines women’s career goals, career barriers, and the challenges they faced at work through case studies and interviews with management practitioners.
Rhabdomyosarcomas in adults and children: an update.

CONTEXT Rhabdomyosarcomas comprise a relatively common diagnostic entity among childhood cancers and a relatively rare one among adult tumors. They may possess a variety of histologies that generally differ among age groups. These lesions appear to be separate biologic entities as well as morphologic categories, with embryonal tumors having genetic lesions related to loss of heterozygosity and aberrant parental imprinting, alveolar tumors containing genetic fusions between PAX and forkhead genes, and pleomorphic tumors showing an accumulation of genetic lesions similar to other adult high-grade sarcomas. OBJECTIVE To present guidelines for diagnosis of rhabdomyosarcoma and recent finding concerning the biology and classification of these lesions. DATA SOURCES Review of recent and older published literature and distillation of the authors' experience. CONCLUSIONS Infants and young children tend to have embryonal rhabdomyosarcomas, adolescents and young adults tend to have alveolar rhabdomyosarcomas, and older adults tend to have pleomorphic rhabdomyosarcomas, although there is some overlap. Newer rare entities, including spindle cell rhabdomyosarcoma and sclerosing rhabdomyosarcoma, have been described in children and adults. Fusion-positive tumors have a distinct molecular signature with downstream activation of a number of myogenic and tumorigenic factors. Genetic testing may be successfully used for diagnosis and may guide therapy in future clinical trials. Differential diagnosis has become simpler than in previous years, because of use of myogenic factors in immunohistochemistry, but classification based solely on histologic features remains challenging.
Tomlinson-Harashima Precoding in Multiuser MIMO Systems with Imperfect Channel State Information

In this paper, the investigation of Tomlinson-Harashima precoding (THP) in downlink multiuser multiple- input multiple-output (MIMO) systems with imperfect channel state information (CSI) is carried out, including two basic structures according to the positions of the diagonal weighted filter: decentralized at the receivers or centralized at the transmitter, which are named as dTHP or cTHP, respectively. The interference power in layers caused by imperfect CSI is first derived, then the comparison between these two THP structures and the linear zero-forcing (ZF) precoding is performed, in terms of interference power and system capacity. Moreover, the independence of the interference powers among layers is presented. Simulation results indicate that with imperfect CSI, THP leads to smaller interference power than linear-ZF, and dTHP leads to larger system capacity than cTHP and linear-ZF.
Use of Sequence Analysis of the NS5B Region for Routine Genotyping of Hepatitis C Virus with Reference to C/E1 and 5′ Untranslated Region Sequences

ABSTRACT Nucleotide sequence analysis of the NS5B region was performed to identify genotypes of 8,479 hepatitis C virus (HCV) RNA-positive patient samples collected in the Canadian province of Quebec. Genotypes could be determined for 97.3% of patients. Genotypes 1 to 6 were found in 59.4, 9.0, 25.7, 3.6, 0.6, and 1.8% of patients, respectively. Two isolates did not classify within the six genotypes. The subtype 1 distribution was 76.7% 1a, 22.6% 1b, and 0.7% others, while the subtype 2 distribution was 31.8% 2a, 47.6% 2b, 10.9% 2c, 4.1% 2i, and 5.6% others. Subtype 3a accounted for 99.1% of genotype 3 strains, while all genotype 5 samples were of subtype 5a. The subtype 4 distribution was 39.2% 4a, 15.4% 4k, 11.6% 4d, 10.2% 4r, and 23.6% others. The subtype 6 distribution was 40.4% 6e, 20.5% 6a, and 39.1% others. The 5′ untranslated region (5′UTR) sequences of subtype 6e were indistinguishable from those of genotype 1. All samples that did not classify within the established subtypes were also sequenced in C/E1 and 5′UTR. C/E1 phylogenetic reconstructions were analogous to those of NS5B. The sequences identified in this study allowed the provisional assignments of subtypes 1j, 1k, 2m, 2r, 3i, 4q, 6q, 6r, and 6s. Sixty-four (0.8%) isolates classifying within genotypes 1 to 6 could not be assigned to one of the recognized subtypes. Our results show that genotyping of HCV by nucleotide sequence analysis of NS5B is efficient, allows the accurate discrimination of subtypes, and is an effective tool for studying the molecular epidemiology of HCV.
Cognitive Function and Serum Hormone Levels Are Associated with Gray Matter Volume Decline in Female Patients with Prolactinomas

Background and objective Cognitive impairments have been reported in patients with hyperprolactinemia; however, there is a lack of knowledge of brain structure alterations relevant to hyperprolactinemia in prolactinomas. Thus, we aimed to identify changes in brain structure in prolactinomas and to determine whether these changes are related to cognitive performance and clinical characteristics. Methods Participants were 32 female patients with prolactinomas and 26 healthy controls (HC) matched for age, sex, education, and handedness. All participants underwent magnetic resonance imaging brain scans, neuropsychological assessments, and clinical evaluations. Voxel-based morphometry analysis was used to identify changes in gray matter volume (GMV). Partial correlation analysis and multiple linear regression were performed to determine the relationship between GMV, cognition, and clinical characteristics. Results Compared to HC, patients with prolactinomas demonstrated a decrease in GMV in the left hippocampus, left orbitofrontal cortex, right middle frontal cortex (MFC), and right inferior frontal cortex (IFC). In addition, patients performed worse than controls on tests for verbal memory and executive function, and this was significantly related to the GMV of the left hippocampus and right MFC, respectively. Moreover, in the patients, we found a negative relationship between serum prolactin levels and the GMV of the left hippocampus and right IFC, whereas a positive relationship was found between the GMV of the left hippocampus and serum levels of estradiol and luteinizing hormone. Conclusion In patients with prolactinomas, specific brain structure abnormalities have been identified and are associated with cognitive impairments and dysfunctional hormones. This study enhances our understanding of brain structure changes that may occur with prolactinomas and provides novel and fundamental evidence for previous behavioral findings relevant to hyperprolactinemia.
Pharmacokinetic modelling of pentoxifylline and lisofylline after oral and intravenous administration in mice

The aim of this study was to develop pharmacokinetic models for pentoxifylline (PTX) and the R(‐)‐enantiomer of the PTX metabolite 1, lisofylline (LSF), in order to identify some factors influencing the absorption of these compounds from the intestines and to clarify mechanisms involved in their non‐linear pharmacokinetics. Serum samples were collected after oral and intravenous administration of PTX and LSF to male CD‐1 mice at two different doses. In addition, both compounds under investigation were coadministered with a modulator of drug transporters, verapamil, and an inhibitor of cytochrome P450 (CYP) 3A4, ketoconazole. Pharmacokinetic analysis revealed that a one‐compartment model with Michaelis‐Menten type absorption and elimination best described the pharmacokinetics of PTX, whereas the LSF concentration‐time data were adequately fitted to a two‐compartment model with a first‐order absorption and Michaelis‐Menten type elimination process. Both coadministered compounds significantly decreased the area under the concentration‐time curve from 0 to 60 min calculated for PTX and increased the value of this parameter for LSF. The results of this study indirectly suggest that saturation of drug transport across intestinal cells and elimination from the central compartment may be responsible for the non‐linear pharmacokinetics of PTX, whereas in the case of LSF, the dose dependency in the pharmacokinetics is solely related to the elimination from the central compartment. It seems that the observed changes in PTX and LSF concentrations after coadministration with verapamil and ketoconazole may be clinically significant, especially after chronic treatment, however further studies are necessary to assess the importance of these interactions in humans.
The IS Manager: A Study of Critical Professional Activities and Skills/Knowledge

This paper utilizes the activity competency model (ACM) to investigate the perceived importance of professional activities and skills/knowledge required of three levels of information system (IS) managers from two types of industries. Carrying an activity out involves the use of a number of different skills and knowledge. The critical activities and the required skills/knowledge for each level of IS management are identified. Our findings indicate that the perceived importance of IS professional activities varies significantly for different levels of management but does not vary significantly for different industries. These results have implications for IS professional succession planning, specialty training and development. They also can serve as guidelines for selecting, performance appraising and recruiting the right IS managers.
Parents' and Caregivers' Concerns About Obesity in Young Children: A Qualitative Study

To address the childhood obesity epidemic, it is necessary to understand parents' and caregivers' concerns and beliefs regarding their children's weight problems and best practices for addressing those concerns. Formative research methods were used to identify obesity-related concerns of Hispanic, Black, and White parents of young children (5–8 years old) in North Carolina. Participants identified challenges at multiple levels of influence. In all groups, participants reported that they had trouble finding enough time to help their children develop healthy lifestyles. Conflicting family priorities and needs often made it difficult to ensure that their children had healthy diets. Children's own diet and activity preferences and their parent or caregiver's inability to adequately guide their choices also contributed to obesigenic behaviors. In addition, many thought that physician and community support for their efforts to manage their children's eating habits was inadequate. Findings from these focus groups suggest that participants would be receptive to positive, multilevel prevention approaches to help their children attain and maintain healthy weights.
Role of peroxisome proliferator-activated receptor-alpha in the mechanism underlying changes in renal pyruvate dehydrogenase kinase isoform 4 protein expression in starvation and after refeeding.

The pyruvate dehydrogenase complex (PDC) occupies a strategic role in renal intermediary metabolism, via partitioning of pyruvate flux between oxidation and entry into the gluconeogenic pathway. Inactivation of PDC via activation of pyruvate dehydrogenase kinases (PDKs), which catalyze PDC phosphorylation, occurs secondary to increased fatty acid oxidation (FAO). In kidney, inactivation of PDC after prolonged starvation is mediated by up-regulation of the protein expression of two PDK isoforms, PDK2 and PDK4. The lipid-activated transcription factor, peroxisome proliferator-activated receptor-alpha (PPAR alpha), plays a pivotal role in the cellular metabolic response to fatty acids and is abundant in kidney. In the present study we used PPAR alpha null mice to examine the potential role of PPAR alpha in regulating renal PDK protein expression. In wild-type mice, fasting (24 h) induced marked up-regulation of the protein expression of PDK4, together with modest up-regulation of PDK2 protein expression. In striking contrast, renal protein expression of PDK4 was only marginally induced by fasting in PPAR alpha null mice. The present results define a critical role for PPAR alpha in renal adaptation to fasting, and identify PDK4 as a downstream target of PPAR alpha activation in the kidney. We propose that specific up-regulation of renal PDK4 protein expression in starvation, by maintaining PDC activity relatively low, facilitates pyruvate carboxylation to oxaloacetate and therefore entry of acetyl-CoA derived from FA beta-oxidation into the TCA cycle, allowing adequate ATP production for brisk rates of gluconeogenesis.
Variable Size Genetic Network Programming with Binomial Distribution

This paper proposes a different type of Genetic Network Programming (GNP) — Variable Size Genetic Network Programming (GNPvs) with Binomial Distribution. In contrast to the individuals with fixed size in Standard GNP, GNPvs will change the size of the individuals and obtain the optimal size of them during evolution. The proposed method defines a new type of crossover to implement the new feature of GNP. The new crossover will select the number of nodes to move from each parent GNP to another parent GNP. The probability of selecting the number of nodes to move satisfies the binomial probability distribution. The proposed method can keep the effectiveness of crossover and improve the performance of GNP. In order to verify the performance of the proposed method, a well-known benchmark problem — Tile-world is used in the simulations. The simulation results show the effectiveness of the proposed method.
Enterprises Cluster Co-existence Model and Stability Analysis

Some similarities are exhibited between species co exist in nature and enterprise cluster in economic life. In this paper logistic equation, from biology, is applied to describe dynamically the process of industry cluster formation through firm's output variable ratio. Two kinds of cluster model, concentration of subcontractors around dominant firm and concentration of simple competitors, are analyzed respectively. Moreover, equilibrium conditions of the two models are given and explained. The main conclusion is that pierce competition is crucial factor for the formation of enterprise cluster.
Autophagy Controls Salmonella Infection in Response to Damage to the Salmonella-containing Vacuole*

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a facultative intracellular pathogen that causes disease in a variety of hosts. S. Typhimurium actively invade host cells and typically reside within a membrane-bound compartment called the Salmonella-containing vacuole (SCV). The bacteria modify the fate of the SCV using two independent type III secretion systems (TTSS). TTSS are known to damage eukaryotic cell membranes and S. Typhimurium has been suggested to damage the SCV using its Salmonella pathogenicity island (SPI)-1 encoded TTSS. Here we show that this damage gives rise to an intracellular bacterial population targeted by the autophagy system during in vitro infection. Approximately 20% of intracellular S. Typhimurium colocalized with the autophagy marker GFP-LC3 at 1 h postinfection. Autophagy of S. Typhimurium was dependent upon the SPI-1 TTSS and bacterial protein synthesis. Bacteria targeted by the autophagy system were often associated with ubiquitinated proteins, indicating their exposure to the cytosol. Surprisingly, these bacteria also colocalized with SCV markers. Autophagy-deficient (atg5-/-) cells were more permissive for intracellular growth by S. Typhimurium than normal cells, allowing increased bacterial growth in the cytosol. We propose a model in which the host autophagy system targets bacteria in SCVs damaged by the SPI-1 TTSS. This serves to retain intracellular S. Typhimurium within vacuoles early after infection to protect the cytosol from bacterial colonization. Our findings support a role for autophagy in innate immunity and demonstrate that Salmonella infection is a powerful model to study the autophagy process.
[Impulsive-compulsive behaviours in a German Parkinson's disease outpatient sample].

BACKGROUND Impulsive-compulsive behaviours (ICBs) are frequent complications of Parkinson's disease (PD), associated with treatment by dopamine agonists (DAs). These include impulse control disorders (ICDs), repetitive behaviour (RB) and the dopamine-dysregulation syndrome (DDS). METHODS A subsample of 72 patients of a large longitudinal study (n = 739) was screened with the Questionnaire for Impulsive-Compulsive Disorders in Parkinson's disease (QUIP). Results were associated with socio-demographic, clinical and neuropsychological parameters. RESULTS A large proportion of the sample reported ICBs (60%), RBs were most frequent (47 %). Patients with ICBs consumed higher doses of DAs (343 ± 177 mg vs. 390 ± 153 mg; p < 0.01). Pramipexole was associated with RB but not ICDs (273 ± 225 mg and 53 ± 106 mg vs. 151 ± 209 mg in patients without ICB). Patients with ICDs reported more dyskinesias (UPDRS IV: 1.78 ± 1.6 vs. 0.55 ± 1.1 points; p = 0.012) and patients with multiple ICBs a longer duration of PD (9.3 ± 5.0 vs. 6.2 ± 4.0 years; p = 0.026) and worse quality of life (PDQ39: 29.9 ± 13.8 vs. 20.3 ± 13.4 points; p = 0.036) compared to patients without any ICB. CONCLUSIONS ICBs are frequent even in outpatients with moderate duration and severity of PD and associated with DA dose. Because of possible serious psychosocial consequences, detecting and managing them is of high importance.