Miracle-dz/UniFAR-FTUs huggingface dataset:
Addressing Gaps in the Management of Chlamydia trachomatis and Neisseria gonorrhoeae in Primary Care: Lessons Learned in a Pilot Intervention Study.

BACKGROUND We aimed to test the acceptability and utility of strategies designed to facilitate the delivery of clinical best practice for patients diagnosed with chlamydia or gonorrhea in primary care. METHODS A nonrandomized pilot intervention study with a historic control period was run over 9 months in six primary health care clinics (2 youth services, 3 low-fee clinics, and 1 student health service) in Wellington, New Zealand. "Study nurses" in participating clinics oversaw the implementation of strategies designed to facilitate partner notification and follow-up for patients diagnosed with chlamydia or gonorrhea. Clinics chose which of 2 approaches they wished to trial-either managing all study processes themselves or drawing on the assistance of an external specialist sexual health advisor. Outcome measures included acceptability and utility of study processes ascertained via structured interviews with study nurses and collection of clinical data. RESULTS Outcomes for 287 patients seen during the intervention were compared with 240 historic controls. Participant views on study processes were positive overall, and all clinics intended to continue all or most of the study processes implemented. During the intervention, substantial improvements were observed in documented patient management (sexual history, partner notification, and outcomes, P < 0.05). Increases were observed in percentages of patients reached for follow-up (74% vs. 26% at baseline, P < 0.05) and partners reported to have been notified (79% vs. 23%, P < 0.05). CONCLUSIONS Nurse-led strategies implemented were deemed acceptable and appeared to facilitate delivery of best practice care for patients diagnosed with bacterial sexually transmitted infections in participating primary care practices.
Comparison of the Effectiveness of Guttman's Cognitive-Systemic Couple Therapy and Mcmaster's Model on Marital Justice of Conflicting Couples

Article Type Analytical Review Purpose: The aim of this study was to compare the effectiveness of Guttman's cognitive-systemic couple therapy and McMaster model on marital justice in conflicting couples. Materials and Methods: The research method was experimental with control group and random assignment. A total of 60 people (30 couples) were selected as a statistical sample by available sampling method from among conflicting couples referring to private counseling and psychology centers in areas 2 and 3 of Tehran. Couples were randomly assigned to three groups (two experimental groups and one control group). First, the pre-test of marital justice was performed on all three groups, then each of the experimental groups underwent training sessions of couple therapy based on Guttman theory and couple therapy based on McMaster theory for ten sessions. At the end of the training sessions, post-test was taken from all three groups. In order to evaluate the durability of the training, one month after the training, both experimental groups were retested. Findings: The results showed that the difference between the mean of marital justice between the stages of pre-test and post-test (p <0.01) and follow-up (p <0.01) was significant. Which showed that the effect of Guttman cognitive couple therapy and Maxmaster model couple therapy on communication patterns of conflicting couples was stable. Conclusion: The results of repeated measures analysis of variance showed that Guttman's systemic-cognitive couple therapy and McMaster model improved marital justice in conflicting couples. Aurhors Dina Abdollahi, Farshad MohsenZadeh, Aboutaleb Seadatee Shamir
The potential role of miR‐29 in health and cancer diagnosis, prognosis, and therapy

miR‐29 family is one of the small noncoding RNAs and has a very important role in many physiologic and pathologic functions through regulating the target genes that play roles in various bioprocesses such as proliferation, survival, apoptosis, and angiogenesis. Thus, we aim to survey the potential of the miR‐29 family in normal model and development and progression of malignancy in this study. In addition, the potential role of miR‐29 family has been studied as the clinical marker for the diagnosis and prognosis of many cancers as the potential targets to treat cancer. Moreover, it was stated in summary that the herbal compounds can regulate miR‐29 family in cancers. Therefore, regulating the expression of the miR‐29 family in a variety of cancers can be a new strategy to obtain better results from cancerous patients’ treatment in the future.
Patient‐Reported Outcome Data From an Early Rheumatoid Arthritis Trial: Opportunities for Broadening the Scope of Treating to Target

Treating early, intensively, and to target leads to rapid disease control, preventing joint damage and loss of function in early rheumatoid arthritis (RA). We report the effect of such an approach on patient‐reported outcomes and explore the contribution of rapid and persistent disease control to well‐being after 1 year of treatment.
Paradoxical Behavior of Organodiselenides: Pro-oxidant to Antioxidant

: Over the years, organodiselenides have emerged as the biologically relevant class of molecules. On the one hand, such compounds are known for pro-oxidant effects leading to toxicity in biological systems. On the other hand, there are growing evidences about their bio-mimetic activities as catalysts such as glutathione peroxidase (GPx)-like activity. Our recent work has explored this paradoxical behavior of diselenides in developing antioxidants and/or anticancer agents. For this purpose, a number of alkyl and aryl diselenides have been evaluated in different biological models. The results have shown that aryl diselenides, in particular pyridinediselenides, altered the ratio of the intracellular thiol redox pairs of glutathione (GSH) and glutathione disulfide (GSSG) towards reduction (antioxidant) rather than oxidation (pro-oxidant) to protect normal cells against radiation damage and to induce cytotoxicity in tumor cells. Further, these studies have also postulated that the intracellular redox state, the level of thioredoxin reductase (TrxR), and reductive intermediates (e.g., selenol and/or selone) might play a very important role in the manifestation of the toxicities of aryl diselenides in cells.
Developmental Programming of Thermonastic Leaf Movement1

The developmentally programmed polarity of the auxin response underlies thermo-induced leaf hyponasty Plants exhibit diverse polar behaviors in response to directional and nondirectional environmental signals, termed tropic and nastic movements, respectively. The ways in which plants incorporate directional information into tropic behaviors is well understood, but it is less well understood how nondirectional stimuli, such as ambient temperatures, specify the polarity of nastic behaviors. Here, we demonstrate that a developmentally programmed polarity of auxin flow underlies thermo-induced leaf hyponasty in Arabidopsis (Arabidopsis thaliana). In warm environments, PHYTOCHROME-INTERACTING FACTOR4 (PIF4) stimulates auxin production in the leaf. This results in the accumulation of auxin in leaf petioles, where PIF4 directly activates a gene encoding the PINOID (PID) protein kinase. PID is involved in polarization of the auxin transporter PIN-FORMED3 to the outer membranes of petiole cells. Notably, the leaf polarity-determining ASYMMETRIC LEAVES1 (AS1) directs the induction of PID to occur predominantly in the abaxial petiole region. These observations indicate that the integration of PIF4-mediated auxin biosynthesis and polar transport, and the AS1-mediated developmental shaping of polar auxin flow, coordinate leaf thermonasty, which facilitates leaf cooling in warm environments. We believe that leaf thermonasty is a suitable model system for studying the developmental programming of environmental adaptation in plants.
The Correlation Between Dry Eyes, Anxiety and Depression: The Sicca, Anxiety and Depression Study

Purpose: To determine whether improvement in the severity of dry eye disease (DED) symptoms correlates with improvement in anxiety and depression. Methods: This prospective interventional case series recruited 45 adults with evidence of DED. Patients were administered the University of North Carolina Dry Eye Management Scale (DEMS), Generalized Anxiety Disorder 7-item scale (GAD-7), and Personal Health Questionnaire Depression Scale (PHQ-8) to evaluate the severity of DED symptoms, anxiety, and depression, respectively. Standard of care treatment was provided for patients for 3 to 6 months, followed by re-administration of the DEMS, GAD-7, and PHQ-9 surveys. Statistical analysis was performed to assess the relationships between changes in survey scores. Results: Participants had a mean age of 65.5 (SD, 13.3) years, and 37 (84.6%) were women. Seven patients were lost to follow-up. DEMS and GAD-7 significantly improved from 5.8 ± 1.8 to 4.6 ± 0.2. 2 (P = 0.01) and from 5.6 ± 5.5 to 3.3 ± 4.6 (P = 0.05), respectively. Changes in DEMS correlated with changes in PHQ-8 (&rgr; = 0.3 P = 0.05), but not with changes in GAD-7 (&rgr; = 0.2 P = 0.3). Changes in DEMS correlated with changes in both PHQ-8 and GAD-7 in the subgroup of patients without prior depression or anxiety diagnosis (&rgr; = 0.6, P = 0.002; &rgr; = 0.4, P = 0.02). A multivariate analysis showed that the relationship between DEMS, PHQ-8, and GAD-7 was independent of a prior diagnosis of depression or anxiety and of the presence of comorbidities. Conclusions: There is a significant correlation between the severity of DED and symptoms of depression and anxiety. Effective DED treatment could have a positive impact on the symptoms of depression and anxiety.
Arts for the Blues – a new creative psychological therapy for depression: a pilot workshop report

Introduction: Research over the last decade has identified both strengths and limitations in the use of routinely prescribed psychological therapies for depression. More recently, a focus on how creative art therapies and ‘arts on prescription’ are developing a growing recognition of their potential additional therapeutic mechanisms for depression. Aim: In an attempt to develop a new therapeutic intervention for depression, this research aligned both the evidence base surrounding the arts on prescription movement, collating these with client-reported helpful factors and preferences for therapeutic interventions. Methods: We developed a framework for a new pluralistic ‘meta-approach’ of therapy for depression, based on; an interdisciplinary thematic synthesis of active ingredients, considered specific features implemented in therapy, and client-reported helpful factors considered to be the broad features or experiences in therapy from both talking therapies and creative approaches. This framework contributed to the development of a pilot workshop entitled Arts for the Blues – A New Creative Psychological Therapy for Depression. An outline of, and evaluation from this workshop is presented in this article. Workshop participants were recruited via a voluntary workshop taking place at a North West Higher Education Institution Arts and Health conference (N = 15). Results: The workshop was evaluated using quantitative measures, with results indicating around a 70% overall satisfaction, followed up with qualitative commentary around areas of good practice and areas for development. These included the positive reflection on the application of creative arts and the multimodal nature of the approach, while others reflected on the potential overwhelming nature of utilising multimodal methods for individuals with depression. Conclusion: Overall feedback from the pilot workshop is discussed in relation to prior research, giving credence to the potential for incorporating arts into therapy.
Organic semiconductor characterisation by scanning tunnelling microscopy and optical spectroscopy

Les proprietes electroniques et d'autoassemblage de deux composes organiques, le DHTAP et le bis-pyrene, ont ete etudiees par microscopie a effet tunnel (STM), sous ultra-vide et a basse temperature. Les proprietes optiques ont ete etudiees par spectroscopie en cavite resonnante (CRD), egalement sous ultravide.La croissance du DHTAP a ete etudiee sur differents metaux nobles. La croissance du bis-pyrene a ete etudiee sur Au(111). Dans chaque cas, les conditions de temperature ideales pour la formation d’une premiere couche organisee ont ete determinees. Differents modeles sont proposes pour les structures observees en premiere couche. La formation de la seconde couche moleculaire a egalement ete etudiee.L'evolution des proprietes optiques, etudie par CRD, du bis-pyrene depose sur du verre borosilicate combinee aux informations obtenues par STM ont permit d’associer ces modifications aux interactions des moments dipolaires de transition des molecules de la premiere et de la seconde couche et on egalement permit de determiner le mode de croissance.Finalement, la possibilite d’induire des modifications chimique de la molecule de DHTAP, integree dans une couche auto-assemblee, en utilisant le courant tunnel du STM a ete observe. Il s’avere que la molecule de DHTAP integree en premiere couche peut subir une double deshydrogenation pour conduire a la formation d’un compose identifie comme etant le 5,7,12,14-tetraazapentacene (TAP). En seconde couche, la formation de deux radicaux, en plus du TAP, a ete observe. La molecule de TAP presente un certain interet du fait qu’elle n’est pas synthetisable pas les methodes conventionnelle de chimie organique.
A Pretreatment Prognostic Score to Stratify Survival in Pancreatic Cancer

Objective: The aim of this study was to develop and validate a pretreatment prognostic score in pancreatic cancer (PDAC). Background: Pretreatment prognostication in PDAC is important for treatment decisions but remains challenging. Available prognostic tools are derived from selected cohorts of patients who underwent resection, excluding up to 20% of patients with exploration only, and do not adequately reflect the pretreatment scenario. Methods: Patients undergoing surgery for PDAC in Heidelberg from July 2006 to June 2014 were identified from a prospective database. Pretreatment parameters were extracted from the database and the laboratory information system. Parameters independently associated with overall survival by uni- and multivariable analyses were used to build a prognostic score. A contemporary cohort from Verona was used for external validation. Results: In 1197 patients, multiple pretreatment parameters were associated with overall survival by univariable analyses. American Society of Anesthesiology classification, carbohydrate antigen 19-9 (CA19-9), carcinoembryonic antigen, C-reactive protein, albumin, and platelet count were independently associated with survival and were used to create the Heidelberg Prognostic Pancreatic Cancer (HELPP)-score. The HELPP-score was closely associated with overall survival (median survival between 31.3 and 4.8 months; 5-year survival rates between 35% and 0%) and was able to stratify survival in subgroups with or without resection as well as in CA19-9 nonsecretors. In the resected subgroup the HELPP-score stratified survival independently of pathological prognostic factors. The HELPP-score was externally validated and was superior to CA19-9 in both the development and validation cohorts. Conclusion: The HELPP-score is a readily available prognostic tool based on pretreatment routine parameters to stratify survival in PDAC independently of resection status and pathological tumor stage.
Arabidopsis DXO1 links RNA turnover and chloroplast function independently of its enzymatic activity

‘RNA-seq data were deposited in the Gene Expression Omnibus Database (https://www.ncbi.nlm.nih.gov/geo/) under accession codes GSE95473 (total RNA-seq for Col-0 plants) and GSE99600 (total RNA-seq for dxo1–2 and small RNAseq for Col-0 and dxo1–2 plants). Coordinates and structure factors were deposited in the Protein Data Bank (https: //www.rcsb.org/) under accession code PDB 6DKN. Molecular modeling data were deposited in the PDB-Devsystem (https://pdb-dev.wwpdb.org/view completed dep/217/detail/). ’
Clustering of Domestic Locations in Layers for the Purpose of Breakout Prevention of Diseases

The aim of this research is to help support Provincial Public Health Offices (PPHOs) by clustering geographical coordinates in terms of latitudes and longitudes and comparing each cluster’s density in order to prevent mosquito-born diseases. In this research we propose using the hierarchically spatial clustering approach with two layers of clustering. We cannot use pre-existing clustering algorithms because they do not fit our purpose due to the naturally breeding area of common house mosquitoes. Thus, a modified method with an additional strict constraint of k-means and k-nearest neighbors were applied to meet this goal. Additional constraint to k-means clustering in the first layer will cause some of the coordinates to un-cluster. Finally, all coordinates must be clustered again to a second layer. However, the constraint of mosquito’s breeding area must be retained thus some falsely alarmed coordinates must be filtered out.
Duckweed as an Agricultural Amendment: Nitrogen Mineralization, Leaching, and Sorghum Uptake.

Excessive N and P in surface waters can promote eutrophication (algae-dominated, low-O waters), which decreases water quality and aquatic life. Duckweed (Lemnaceae), a floating aquatic plant, rapidly absorbs N and P from water and its composition shows strong potential as a soil amendment. Therefore, it may be used to transfer N and P from eutrophic water bodies to agricultural fields. In this work, dried duckweed was incorporated into agricultural soil in microcosm, column, and field tests to evaluate biological N cycling, nutrient retention, and crop yield compared with compost, diammonium phosphate (DAP), and an amendment-free control. In microcosm tests, 25 ± 13% of duckweed N was mineralized, providing on average less mineral N than DAP (107 ± 21%), but more than compost (11 ± 12%). In columns, duckweed treatments leached only 2% of the N added, significantly less than DAP, which leached 60% of its N. Compared with the control, DAP leached significantly more phosphate (78%), whereas duckweed and compost treatments leached less (56 and 27%, respectively). Crop yield, as well as runoff N and P, were measured in field tests growing forage sorghum [ (L.) Moench. ]. Although less total N was applied to duckweed plots than to DAP plots (75 vs. 130 kg ha, respectively), duckweed was found to retain 30% more total mineral N in a tilled agricultural field than DAP, while supporting a comparable yield. These tests indicate that duckweed may provide a sustainable source of N and P for agriculture.
A lymph node metastasis‐related protein‐coding genes combining with long noncoding RNA signature for breast cancer survival prediction

Integrating protein‐coding gene (PCG) with long noncoding RNA (lncRNA) expression profiles, our aim is to identify a multidimensional transcriptome model that can predict individual prognosis of patients with breast cancer (BRCA). After diverse bioinformatics and statistical analyses, we obtained gene expression profiles of 1,016 BRCA samples from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) database, and constructed a prognostic signature, which is composed of four PCGs (TFCP2, LRRC75B, PROSER2, and STOML1) and one lncRNA (AL355592.1). In the training set, the multidimensional transcriptome signature could part patients with BRCA into two groups with different survival, defined as high‐ and low‐risk group by Kaplan–Meier (KM) analysis (p < 0.001). In the other five validation datasets, the PCG‐lncRNA signature showed a similar predictive performance in BRCA by KM (p < 0.05). The prognostic independence for the PCG‐lncRNA model was verified by the multivariable Cox regression analysis. Because Chi‐squared test showed the signature was associated with lymph node metastasis status, stratification analysis found that it could further subdivide lymph node metastasis status more precisely in BRCA. The function analysis suggested that the genes from the signature were associated with immunity‐related pathways. In summary, we constructed a PCG‐lncRNA signature, which could accurately predict survival in patients with BRCA.
A directed learning strategy integrating multiple omic data improves genomic prediction

Summary Genomic prediction (GP) aims to construct a statistical model for predicting phenotypes using genome‐wide markers and is a promising strategy for accelerating molecular plant breeding. However, current progress of phenotype prediction using genomic data alone has reached a bottleneck, and previous studies on transcriptomic and metabolomic predictions ignored genomic information. Here, we designed a novel strategy of GP called multilayered least absolute shrinkage and selection operator (MLLASSO) by integrating multiple omic data into a single model that iteratively learns three layers of genetic features (GFs) supervised by observed transcriptome and metabolome. Significantly, MLLASSO learns higher order information of gene interactions, which enables us to achieve a significant improvement of predictability of yield in rice from 0.1588 (GP alone) to 0.2451 (MLLASSO). In the prediction of the first two layers, some genes were found to be genetically predictable genes (GPGs) as their expressions were accurately predicted with genetic markers. Interestingly, we made three dramatic discoveries for the GPGs: (i) GPGs are good predictors for highly complex traits like yield; (ii) GPGs are mostly eQTL genes (cis or trans); and (iii) trait‐related transcriptional factor families are enriched in GPGs. These findings support the notion that learned GFs not only are good predictors for traits but also have specific biological implications regarding regulation of gene expressions. To differentiate the new method from conventional GP models, we called MLLASSO a directed learning strategy supervised by intermediate omic data. This new prediction model appears to be more reliable and more robust than conventional GP models.
Coupling of the Na+/K+-ATPase to Ankyrin B controls Na+/Ca2+ exchanger activity in cardiomyocytes.

AIMS Ankyrin B (AnkB) is an adaptor protein that assembles Na+/K+-ATPase (NKA) and Na+/Ca2+ exchanger (NCX) in the AnkB macromolecular complex. Loss-of-function mutations in AnkB cause the AnkB syndrome in humans, characterized by ventricular arrhythmias and sudden cardiac death. It is unclear to what extent NKA binding to AnkB allows regulation of local Na+ and Ca2+ domains and hence NCX activity. METHODS AND RESULTS To investigate the role of NKA binding to AnkB in cardiomyocytes, we synthesized a disruptor peptide (MAB peptide) and its AnkB binding ability was verified by pulldown experiments. As opposed to control, the correlation between NKA and NCX currents was abolished in adult rat ventricular myocytes (ARVMs) dialyzed with MAB peptide, as well as in cardiomyocytes from AnkB+/- mice. Disruption of NKA from AnkB (with MAB peptide) increased NCX-sensed cytosolic Na+ concentration, reduced Ca2+ extrusion through NCX, and increased frequency of Ca2+ sparks and Ca2+ waves without concomitant increase in Ca2+ transient amplitude or SR Ca2+ load, suggesting an effect in local Ca2+ domains. Selective inhibition of the NKAα2 isoform abolished both the correlation between NKA and NCX currents and the increased rate of Ca2+ sparks and waves following NKA/AnkB disruption, suggesting that an AnkB/NKAα2/NCX domain controls Ca2+ fluxes in cardiomyocytes. CONCLUSIONS NKA binding to AnkB allows ion regulation in a local domain, and acute disruption of the NKA/AnkB interaction using disruptor peptides lead to increased rate of Ca2+ sparks and waves. The functional effects were mediated through the NKAα2 isoform. Disruption of the AnkB/NKA/NCX domain could be an important pathophysiological mechanism in the AnkB syndrome.