Miracle-dz/UniFAR-FTUs huggingface dataset:
p53 checkpoint ablation exacerbates the phenotype of Hinfp dependent histone H4 deficiency

Histone Nuclear Factor P (HINFP) is essential for expression of histone H4 genes. Ablation of Hinfp and consequential depletion of histones alter nucleosome spacing and cause stalled replication and DNA damage that ultimately result in genomic instability. Faithful replication and packaging of newly replicated DNA are required for normal cell cycle control and proliferation. The tumor suppressor protein p53, the guardian of the genome, controls multiple cell cycle checkpoints and its loss leads to cellular transformation. Here we addressed whether the absence of p53 impacts the outcomes/consequences of Hinfp-mediated histone H4 deficiency. We examined mouse embryonic fibroblasts lacking both Hinfp and p53. Our data revealed that the reduced histone H4 expression caused by depletion of Hinfp persists when p53 is also inactivated. Loss of p53 enhanced the abnormalities in nuclear shape and size (i.e. multi-lobed irregularly shaped nuclei) caused by Hinfp depletion and also altered the sub-nuclear organization of Histone Locus Bodies (HLBs). In addition to the polyploid phenotype resulting from deletion of either p53 or Hinfp, inactivation of both p53 and Hinfp increased mitotic defects and generated chromosomal fragility and susceptibility to DNA damage. Thus, our study conclusively establishes that simultaneous loss of both Hinfp and the p53 checkpoint is detrimental to normal cell growth and may predispose to cellular transformation.
enetic analysis of the S 100 B gene in Chinese patients with arkinson disease

There is growing evidence that genetic abnormalities play an important role in the pathogenesis of Parkinson disease (PD). At least 18 genetic loci and 13 disease-related genes for parkinsonism have been identified. The S100 calcium-binding beta (S100B), which is expressed and secreted by astrocytes, has been found to be associated with PD. To evaluate whether the S100B variants are related to PD in Chinese Han ccepted 14 September 2013
Accommodative microfluctuation in asthenopia caused by accommodative spasm.

BACKGROUND Although many patients complain of eye fatigue caused by accommodative spasm, there have been no reports of a good objective examination method to diagnose it. PURPOSE The spectral power of the high frequency component of the accommodative microfluctuation (spectral power of HFC) differs according to the constrictive degree of the accommodation. In this paper, we expatiated upon our previously reported analyzing processes of the spectral power of HFC, and we investigated the relationship between normal subjects and subjects with asthenopia. METHOD The accommodative microfluctuation were recorded when the subjects were looking at a stable target. The waves of the accommodative microfluctuation were analyzed by FFT. RESULTS The spectral power of HFC for the distant target was 50-60 in the subjects with normal vision, but it was higher in the subjects with asthenopia. CONCLUSION Our results suggested that the ciliary muscle was also actively working in asthenopia caused by accommodative spasm even if the patient was looking at a distant target.
Liberal Versus Restrictive Fluid Administration to Improve Recovery After Laparoscopic Cholecystectomy: A Randomized, Double-Blind Study

Objective:The objective of this study was to investigate the effects of 2 levels of intraoperative fluid administration on perioperative physiology and outcome after laparoscopic cholecystectomy. Summary Background Data:Intraoperative fluid administration is variable as a result of limited knowledge of physiological and clinical effects of different fluid substitution regimens. Methods:In a double-blind study, 48 ASA I–II patients undergoing laparoscopic cholecystectomy were randomized to 15 mL/kg (group 1) or 40 mL/kg (group 2) intraoperative administration of lactated Ringer’s solution (LR). All other aspects of perioperative management as well as preoperative fluid status were standardized. Primary outcome parameters were assessed repeatedly for the first 24 postoperative hours and included pulmonary function (spirometry), exercise capacity (submaximal treadmill test), cardiovascular hormonal responses, balance function, pain, nausea and vomiting, recovery, and hospital stay. Results:Intraoperative administration of 40 mL/kg compared with 15 mL/kg LR led to significant improvements in postoperative pulmonary function and exercise capacity and a reduced stress response (aldosterone, antidiuretic hormone, and angiotensin II). Nausea, general well-being, thirst, dizziness, drowsiness, fatigue, and balance function were also significantly improved, as well as significantly more patients fulfilled discharge criteria and were discharged on the day of surgery with the high-volume fluid substitution. Conclusions:Intraoperative administration of 40 mL/kg compared with 15 mL/kg LR improves postoperative organ functions and recovery and shortens hospital stay after laparoscopic cholecystectomy.
Robust Finite Volume Schemes for Simulating Waves in the Solar Atmosphere

We present well-balanced high-resolution finite volume schemes for simulating waves in the outer solar atmosphere. The schemes approximate the stratified MHD equations with an upwind discretization of the Godunov-Powell source term and a locally hydrostatic pressure reconstruction that preserves discrete steady states. The paper summarizes recent articles [4, 5, 6].
Satellite cell activity is required for hypertrophy of overloaded adult rat muscle

Hypertrophy of extensor digitorum longus muscle, overloaded by the removal of the synergist tibialis anterior (TA) muscle, in growing rats is inhibited if endogenous satellite cells are sterilized by exposure to irradiation. However, normal muscle growth is not eliminated, only diminished. To test whether irradiated, overloaded muscle can hypertrophy in the absence of normal growth‐related stimuli, experiments were conducted on mature rats. TA muscle ablation caused hypertrophy of EDL muscle, characterized by a significant increase in muscle mass and the size of type IIx and type IIb fibers, and a proportional increase the number of myonuclei. When ablation was preceded by irradiation hypertrophy did not occur. The results indicate that satellite cell activation, division, and fusion is necessary for compensatory hypertrophy of fully mature muscle, and may be important to the understanding of the limits of recovery of inherited muscle myopathies treated by myogenic cell implantation. © 1994 John Wiley & Sons, Inc.
To my beloved family

Emami Khoonsari, P. 2017. Proteomics Studies of Subjects with Alzheimer’s Disease and Chronic Pain. Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1385. 82 pp. Uppsala: Acta Universitatis Upsaliensis. ISBN 978-91-513-0111-2. Alzheimer’s disease (AD) is a neurodegenerative disease and the major cause of dementia, affecting more than 50 million people worldwide. Chronic pain is long-lasting, persistent pain that affects more than 1.5 billion of the world population. Overlapping and heterogenous symptoms of AD and chronic pain conditions complicate their diagnosis, emphasizing the need for more specific biomarkers to improve the diagnosis and understand the disease mechanisms. To characterize disease pathology of AD, we measured the protein changes in the temporal neocortex region of the brain of AD subjects using mass spectrometry (MS). We found proteins involved in exo-endocytic and extracellular vesicle functions displaying altered levels in the AD brain, potentially resulting in neuronal dysfunction and cell death in AD. To detect novel biomarkers for AD, we used MS to analyze cerebrospinal fluid (CSF) of AD patients and found decreased levels of eight proteins compared to controls, potentially indicating abnormal activity of complement system in AD. By integrating new proteomics markers with absolute levels of Aβ42, total tau (t-tau) and p-tau in CSF, we improved the prediction accuracy from 83% to 92% of early diagnosis of AD. We found increased levels of chitinase-3-like protein 1 (CH3L1) and decreased levels of neurosecretory protein VGF (VGF) in AD compared to controls. By exploring the CSF proteome of neuropathic pain patients before and after successful spinal cord stimulation (SCS) treatment, we found altered levels of twelve proteins, involved in neuroprotection, synaptic plasticity, nociceptive signaling and immune regulation. To detect biomarkers for diagnosing a chronic pain state known as fibromyalgia (FM), we analyzed the CSF of FM patients using MS. We found altered levels of four proteins, representing novel biomarkers for diagnosing FM. These proteins are involved in inflammatory mechanisms, energy metabolism and neuropeptide signaling. Finally, to facilitate fast and robust large-scale omics data handling, we developed an einfrastructure. We demonstrated that the e-infrastructure provides high scalability, flexibility and it can be applied in virtually any fields including proteomics. This thesis demonstrates that proteomics is a promising approach for gaining deeper insight into mechanisms of nervous system disorders and find biomarkers for diagnosis of such diseases.
Doctor-patient communication: the Toronto consensus statement.

This report was compiled on behalf of the staff at the Northern General Hospital, Sheffield, the staff at the Royal Hallamshire Hospital, Sheffield, and the many people who helped at the scene. The efforts of hundreds of professionals and volunteers were vital and greatly appreciated. We thank Neil Appleyard, David Edbrooke, David Dawson, Stuart Yates, Ian Winston, John Duncan, G A Baker, Charlie Cooper, Kath Sherry, Tim Shaw, and A Moss for access to the neuropsychological reports on some of the survivors.
Mutational Analysis of Vpr-Induced G2Arrest, Nuclear Localization, and Cell Death in Fission Yeast

ABSTRACT Cell cycle G2 arrest, nuclear localization, and cell death induced by human immunodeficiency virus type 1 Vpr were examined in fission yeast by using a panel of Vpr mutations that have been studied previously in human cells. The effects of the mutations on Vpr functions were highly similar between fission yeast and human cells. Consistent with mammalian cell studies, induction of cell cycle G2 arrest by Vpr was found to be independent of nuclear localization. In addition, G2 arrest was also shown to be independent of cell killing, which only occurred when the mutant Vpr localized to the nucleus. The C-terminal end of Vpr is crucial for G2 arrest, the N-terminal α-helix is important for nuclear localization, and a large part of the Vpr protein is responsible for cell killing. It is evident that the overall structure of Vpr is essential for these cellular effects, as N- and C-terminal deletions affected all three cellular functions. Furthermore, two single point mutations (H33R and H71R), both of which reside at the end of each α-helix, disrupted all three Vpr functions, indicating that these two mutations may have strong effects on the overall Vpr structure. The similarity of the mutant effects on Vpr function in fission yeast and human cells suggests that fission yeast can be used as a model system to evaluate these Vpr functions in naturally occurring viral isolates.
Are admission procalcitonin levels universal mortality predictors across different medical emergency patient populations? Results from the multi-national, prospective, observational TRIAGE study

Abstract Background: Procalcitonin (PCT), an inflammatory blood biomarker, is well studied in infectious diseases. Its prognostic value in unselected emergency department (ED) patients remains yet undefined. Herein, we investigated association of admission PCT levels and mortality in a large, international-multicenter ED patient cohort. Methods: We prospectively enrolled 6970 unselected, consecutive, adult, medical patients seeking ED care in three tertiary-care hospitals in Switzerland, France and the USA. We used multivariable logistic regression models to examine association of admission PCT levels (as a continuous predictor and across cut-offs) and 30-day mortality. We also investigated subgroup effects by main diagnosis, comorbidities and clinical features at presentation. Results: During the 30-day follow-up, 328 (4.7%) participants died. Mortality increased stepwise within higher PCT cut-offs (0.05, 0.1, 0.25, 0.5 ng/mL) from 1%, 3%, 7%, 13% to 15%, respectively. This association was also confirmed in a fully-adjusted model including age, gender, main symptom, main diagnosis and vital parameters on admission. Receiver operating characteristic (ROC) curve analysis showed that PCT differentiated well between survivors and non-survivors in the overall cohort (area under ROC curve [AUC] 0.75) with best results for patient with metabolic (AUC: 0.85) and cardiovascular disease (AUC: 0.82). Addition of PCT also improved the prognostic accuracy of the quick sequential organ failure assessment (qSOFA) score from an AUC of from 0.61 to 0.76 (p<0.001). Results were similar for other secondary endpoints including intensive care unit (ICU) admission and hospital readmission. Conclusions: In this large and heterogenous medical ED patient cohort, admission PCT was a strong and independent outcome predictor for 30-day mortality across different medical diagnoses independent of underlying infection. PCT may help to improve risk stratification in unselected medical ED patients.
Augmentation Strategies in Electroconvulsive Therapy

Electroconvulsive therapy (ECT) is a highly effective treatment, but strategies to enhance therapeutic outcomes are occasionally needed. This review examines the evidence for approaches used for enhancing seizure production: hyperventilation, pretreatment with xanthines, and use of remifentanil or ketamine in ECT anesthesia. Hyperventilation may be a useful strategy to enhance seizure production, but its effects on ECT outcomes have not been systematically studied and require further research. Pretreatment with caffeine, theophylline or aminophylline (xanthines) prolongs the duration of ECT seizures but has not been clearly shown in controlled trials to increase efficacy. Caution is also warranted because their use may be associated with significant adverse effects. There are case reports of the usefulness of remifentanil in assisting seizure induction by reducing the dose of barbiturate anesthetic required, but there are no controlled data on whether it independently enhances efficacy outcomes. Preliminary evidence suggests that ketamine and ECT may have synergistic antidepressant effects, although this needs to be further examined in randomized controlled trials.
CLUSTER BASED ANONYMIZATION FOR PRIVACY PRESERVATION IN SOCIAL NETWORK DATA COMMUNITY

Nowadays, social network data has been made publicly available, analyzed and utilized in one way or another. Since, social network data are publically available it leads to an important issue in Privacy preserving. This paper explores the existing anonymization techniques for privacy preserving publishing of social network data. The problem formulation is done by considering privacy, data utility and knowledge as dimensions in this paper. This paper describes about clustering- based anonymization methods for privacy preservation .
Cancer-Related Psychological Distress: A Concept Analysis.

BACKGROUND Cancer-related psychological distress, as a concept, has limited research literature substantiation. Several studies report that patients with cancer suffer from significant psychological distress; however, the description of the concept of cancer-related psychological distress has not been clearly described. Theoretical work based on the concept is also unclear. OBJECTIVES This article is a report on the concept of cancer-related psychological distress to clarify the concept as separate from non-cancer-related psychological distress and promote the use of the term in nursing practice and research across the cancer trajectory. METHODS This article used a content analysis to examine the literature. The literature review for this article used CINAHL®, PsycINFO®, and PubMed to search publications from 1999-2016. FINDINGS Content analysis of the literature revealed that the term psychological distress was used often with regard to distress in patients with cancer, but the concept of cancer-related psychological distress was not clearly defined. Four attributes encompass the concept of cancer-related psychological distress.
The Quantitative Analysis of Morphosyntactic Variation: Constructing and Quantifying the Denominator

At its inception, the sociolinguistic enterprise focused on research on phonological variability and only later broadened its remit to include ‘variability [that] occurs and can be dealt with at level of grammar above (and beyond) the phonological’ (Sankoff 1972:45). This extension, however, has not been without controversy and continues to fuel heated debates in the field. This article addresses some of the contentious issues that quantitative research on (morpho-)syntactic variation has brought about. Can we operationalise variation in the field of morphosyntax as a sociolinguistic variable? If yes, how do we define such a variable? Is morphosyntax constrained in the same way as variation in the sound system? How do we quantify variability in the field of morphosyntax in an accountable manner? What do we quantify out of? I also introduce some of the methods used by researchers dealing with non-phonological variation, focusing especially on the quantification of morphosyntactic and discourse variables.
On the effect of functionalizer chain length and water content in polyethylene/silica nanocomposites: Part I — Dielectric properties and breakdown strength

A series of nanoparticles was prepared by functionalizing a commercial nanosilica with alkylsilanes of varying alkyl tail length, from propyl to octadecyl. By using a constant molar concentration of silane, the density of alkyl groups attached to each system should be comparable. The effect of chain length on the structure of the resulting nanosilica/polyethylene nanocomposites was examined and comparison with an unfilled reference system revealed that, other than through a weak nucleating effect, the inclusion of the nanosilica does not affect the matrix structure. Since water interacts strongly with applied electric fields, water was used as a dielectric probe in conjunction with dielectric spectroscopy to examine the effect of the nanofiller and its surface chemistry on the system. Sets of samples were prepared through equilibrating under ambient conditions, vacuum drying and water immersion. While the water content of the unfilled polymer was not greatly affected, the water content of the nanocomposites varied over a wide range as a result of water accumulation, in a range of states, at nanoparticle interfaces. The effect of water content on breakdown behavior was also explored and, in the unfilled polymer, the breakdown strength was found to depend little on exposure to water (∼13% reduction). In all the nanocomposites, the increased propensity for these systems to absorb water meant that the breakdown strength was dramatically affected (>66% reduction).
Running mileage, movement mileage, and fitness in male U.S. Navy recruits.

PURPOSE The purpose of this study was to determine whether there is a relationship between overall fitness improvement and varying amounts of running and movement mileage. METHODS Subjects were male U.S. Navy recruits (N = 1703, 25 divisions), ages 17-35 yr (mean age = 20.1 +/- 2.9 yr), who attended boot camp from April 1996 through August 1996. During the first week of training, recruits performed a 1.5-mile run to determine baseline fitness levels. The results from the initial run were compared with a final 1.5-mile run conducted 6 wk later. RESULTS Based on an age-adjusted fitness scale for a 1.5-mile run time, about one third of the recruits began recruit training in "Excellent-Superior" condition (N = 558), one third began in "Good" condition (N = 582), and one third began in "Poor-Fair" condition (N = 563). Running mileage among divisions ranged from 11.5 to 43.5 miles for the entire 7-wk training period (mean = 22.7 +/- 7.2 miles; 8-22 run days, mean = 13 +/- 4 d). In addition to running, the divisions accumulated many movement miles (110-202 miles; mean = 145 +/- 26 miles) while marching in formation. Recruits who began training in Poor-Fair condition improved the most with an average decrease in run time of 1:55 +/- 1:06 min (15.6% improvement). The Good group improved by 47 +/- 37 s (7.3% improvement), and the Excellent-Superior group improved by 17 +/- 32 s (2.9% improvement). CONCLUSION The magnitude of fitness improvement, as measured by run time improvement, was directly related to baseline fitness level but not related to movement mileage or high-intensity run mileage accrued during training.
Ambipolar nano-crystalline-silicon TFTs with submicron dimensions and reduced threshold voltage shift

Hydrogenated nano-crystalline-silicon (nc-Si) thin-film transistors (TFTs) are primary candidates for use in neuromorphic circuits and systems [1]. Such devices can be fabricated at low temperatures and over large areas, allowing cheap processing and three-dimensional integration with CMOS structures. The major drawbacks of nc-Si TFTs include low carrier mobility, threshold voltage (VT) shift under bias stress and lack of p-channel operation due to unintentional n-type doping by oxygen impurity present in the nc-Si layer [2]. We have fabricated nc-Si TFTs that minimize all the above drawbacks, and are thus well suited for use in neuromorphic applications.
INTERSENSORY FACILITATION IN RAPID SINGLE-JOINT VOLUNTARY ACTIVATION AND CANCELLATION OF ARM MOVEMENTS

The ability to initiate and cancel actions is a basic requirement for motor control in humans. Rapid movements to stationary targets over single joints are characterized by triphasic bursts of electromyographic (EMG) activity. While analysis of reaction time in motor activation tasks, in relation to different modalities of sensory inputs, has studied, its diametrically opposite task of motor cancellation has not been adequately addressed. We studied 9 normal right-handed subjects using biceps (agonist) and triceps (antagonist) EMG recordings. Each underwent 3 motor activation and 3 motor cancellation tasks to light, sound and dual stimuli (6 blocks). The former consisted of ballistic elbow flexion over 45 degrees, while the latter involved dropping of the forearm from a 45-degree elbow flexion angle. For motor activation, onset latencies and duration of agonist (Lat1, Dur1) and antagonist (Lat2, Dur2) muscles were recorded. For motor cancellation, onset latencies and duration of agonist (Lat1 only) and antagonist (Lat2, Dur2) were noted. Motor cancellation showed significantly shorter Dur2 EMG bursts (p < .0005) for all 3 stimuli conditions. Lat1 and Lat2 demonstrated significant correlation (p < 0.0005 for all), with the exception of dual stimulus condition during motor cancellation (p = 0.089). While dual stimulus during motor cancellation resulted in significantly shorter Lat2 (p = .013) in comparison with light and sound stimuli, this was not evident for motor activation tasks. The findings suggest that while a common central program exists for executing motor activation and cancellation, generation of antagonist activity in the latter may involve distinct neural pathways specifically robust to the effects of intersensory facilitation. This is discussed in relation to reciprocal motor oscillatory activity manifestations at the level of single joint movements.