Miracle-dz/UniFAR-FTUs huggingface dataset:
Older adult US Medicare beneficiaries with untreated obstructive sleep apnea are heavier users of health care than matched control patients.

STUDY OBJECTIVES To examine the effect of untreated obstructive sleep apnea (OSA) on health care utilization (HCU) and costs among a nationally representative sample of Medicare beneficiaries. METHODS Our data source was a random 5% sample of Medicare administrative claims data for years 2006-2013. OSA was operationalized as (1) receipt of one or more International Classification of Disease, Version 9, Clinical Modification diagnostic codes for OSA in combination with (2) initiation of OSA treatment with either continuous positive airway pressure or oral appliance (OA) therapy. First, HCU and costs were assessed during the 12 months prior to treatment initiation. Next, these HCU and costs were compared between beneficiaries with OSA and matched control patients without sleep-disordered breathing using generalized linear models. RESULTS The final sample (n = 287,191) included 10,317 beneficiaries with OSA and 276,874 control patients. In fully adjusted models, during the year prior to OSA diagnosis and relative to matched control patients, beneficiaries with OSA demonstrated increased HCU and higher mean total annual costs ($19,566, 95% confidence interval [CI] $13,239, $25,894) as well as higher mean annual costs across all individual points of service. Inpatient care was associated with the highest incremental costs (ie, greater than control patients; $15,482, 95% CI $8,521, $22,443) and prescriptions were associated with the lowest incremental costs (ie, greater than control patients; $431, 95% CI $339, $522). CONCLUSIONS In this randomly selected and nationally representative sample of Medicare beneficiaries and relative to matched control patients, individuals with untreated OSA demonstrated increased HCU and costs across all points of service.
Increased mitochondrial apoptotic priming with targeted therapy predicts clinical response to re‐induction chemotherapy

Most patients with relapsed or refractory (R/R) acute myeloid leukemia (AML) do not benefit from current re‐induction or approved targeted therapies. In the absence of targetable genetic mutations, there is minimal guidance on optimal treatment selection particularly in the R/R setting highlighting an unmet need for clinically useful functional biomarkers. Blood and bone marrow samples from patients treated on two clinical trials were used to test the combination of lenalidomide (LEN) and MEC (mitoxantrone, etoposide, and cytarabine) chemotherapy in R/R AML patients. The bone marrow samples were available to test the clinical utility of the mitochondrial apoptotic BH3 and dynamic BH3 profiling (DBP) assays in predicting response, as there was no clear genetic biomarker identifying responders. To test whether LEN‐induced mitochondrial priming predicted clinical response to LEN‐MEC therapy, we performed DBP on patient myeloblasts. We found that short‐term ex vivo treatment with lenalidomide discriminated clinical responders from non‐responders based on drug‐induced change in priming (delta priming). Using paired patient samples collected before and after clinical LEN treatment (prior to MEC dosing), we confirmed LEN‐induced increased apoptotic priming in vivo, suggesting LEN enhanced vulnerability of myeloblasts to cytotoxic MEC chemotherapy. This is the first study demonstrating the potential role of DBP in predicting clinical response to a combination regimen. Our findings demonstrate that functional properties of relapsed AML can identify active therapies.
Integrative Network Analysis Linking Clinical Outcomes With Environmental Exposures and Molecular Variations in Service Personnel Deployed to Balad and Bagram.

OBJECTIVE To develop a computational approach to link clinical outcomes with environmental exposures and molecular variations measured in Department of Defense (DOD) serum-repository samples. METHODS International Classification of Diseases, Ninth Division codes which corresponded to cardiopulmonary symptoms for service personnel were selected to test for associations with deployment-related inhalation hazards and metabolomics, micro-RNA, cytokine, plasma markers, and environmental exposure analyses for corresponding samples. xMWAS and Mummichog were used for integrative network and pathway analysis. RESULTS Comparison between 41 personnel exhibiting new cardio-pulmonary diagnoses after deployment start-date to 25 personnel exhibiting no symptoms identified biomarkers associated with cardiopulmonary conditions. Integrative network and pathway analysis showed communities of clinical, molecular, and environmental markers associated with fatty acid, lipid, nucleotide, and amino acid metabolism pathways. CONCLUSIONS The current proof of principle study establishes a computational framework for integrative analysis of deployment-related exposures, molecular responses, and health outcomes.
Using Small-Sided Games in Field Hockey: Can They Be Used to Reach Match Intensity?

Abstract Duthie, GM, Thomas, EJ, Bahnisch, J, Thornton, HR, and Ball, K. Using small-sided games in field hockey: Can they be used to reach match intensity? J Strength Cond Res 36(2): 498–502, 2022—Small-sided games (SSGs) are commonly used in field hockey to replicate or overload physical and tactical competition demands; however, little evidence is available regarding if specific SSG formats achieve this. This research investigated how speed and acceleration differed between 5-minute SSG formats (2v2, 3v3, and 4v4) and also compared with competition. Elite male field hockey athletes (n = 10) participated in the SSGs with standard rules, on pitches of 30 × 20, 35 × 25, and 40 × 30 m, respectively (∼150 m2 per athlete excluding goal keepers). Global Positioning System devices measured speed (m·min−1) and acceleration (m·s−2). Differences between mean speed and acceleration during SSGs and 5-minute maximal mean values from competition (calculated using moving averages) were quantified using linear mixed models and described using standardized effect sizes (ES) and 90% confidence intervals. Between SSG formats, there were nonsubstantial differences for mean speed and acceleration (ES range −0.4 to 0.8). Compared with competition, mean speed was substantially lower for all SSG formats (ES range −1.4 to −1.8), whereas mean acceleration was higher (ES range 3.9–4.8). The SSG formats examined had a lower mean speed and higher mean acceleration than the maximal mean observed in competition. Therefore, these SSGs are an effective training drill for the development and overload of acceleration. However, manipulating SSG constraints to expose athletes to the maximal mean competition speed is required.
Converting Coherence to Signal-to-noise Ratio for Enhancement of Adaptive Ultrasound Imaging

High-frame-rate ultrasound is an emerging technique for functional ultrasound imaging. However, the lateral spatial resolution and contrast in high-frame-rate ultrasound with an unfocused transmit beam are inherently lower than those in conventional ultrasonic imaging based on the line-by-line acquisition using a focused ultrasonic beam because of the low directivity of the transmit beam. Coherence-based beamforming methods were introduced in ultrasound imaging for improvement of image quality. Such methods improve the lateral spatial resolution using the coherence among ultrasonic echo signals received by individual transducer elements. In this study, a new method based on the signal-to-noise ratio (SNR) among the element echo signals was developed for enhancement of the effect of the coherence factor (CF), which was previously developed for improvement in spatial resolution and contrast. In the proposed method, a new factor, namely, SNR factor, was introduced, and the relationship between the previously developed CF and SNR factor was discussed. The proposed method was implemented in plane wave imaging, and the performance was evaluated by simulated and phantom experiments. In simulation, the lateral spatial resolution and contrast obtained with the conventional CF were 0.23 mm and 47.0 dB, respectively, which were significantly better than 0.39 mm and 15.3 dB obtained by conventional delay-and-sum (DAS) beamforming. Using the proposed method, the lateral spatial resolution and contrast were further improved to 0.12 mm and 69.8 dB, respectively. Similar trends were found also in phantom experiments.
Individualization Recommended Model of Gaussian Symmetric Optimization in Independent Increment Learning

Targeting at the problem of inaccuracy in classification of common sorting algorithms in information individualization recommended applications, the letter put forward individualization recommended model of Gaussian symmetric optimization in independent increment learning. First, we apply optimal method of confidence coefficient based on neighbor K, making use of mass of data without name tag information, combining a small number of data with name tag information. Then we employ dichotomy optimal method on the basis of maximum gap, selecting data with highest level of confidence and Gaussian symmetric distribution to conduct independent increment learning so that the quality of individualization recommendation can be improved. The instance simulation proves that this individualization recommended model of Gaussian symmetric optimization in independent increment learning has higher classified accuracy and can be better applied in individualized application.
Conditional Deletion of LRP1 Leads to Progressive Loss of Recombined NG2-Expressing Oligodendrocyte Precursor Cells in a Novel Mouse Model

The low-density lipoprotein receptor-related protein 1 (LRP1) is a transmembrane receptor, mediating endocytosis and activating intracellular signaling cascades. LRP1 is highly expressed in the central nervous system (CNS), especially in oligodendrocyte precursor cells (OPCs). Previous studies have suggested LRP1 as a regulator in early oligodendrocyte development, repair of chemically induced white matter lesions, and cholesterol homeostasis. To circumvent embryonic lethality observed in the case of global LRP1 deletion, we generated a new inducible conditional knockout (KO) mouse model, which enabled an NG2-restricted LRP1 deficiency (NG2-CreERT2ct2/wtxR26eGFPflox/floxxLRP1flox/flox). When characterizing our triple transgenic mouse model, we noticed a substantial and progressive loss of recombined LRP1-deficient cells in the oligodendrocyte lineage. On the other hand, we found comparable distributions and fractions of oligodendroglia within the Corpus callosum of the KO and control animals, indicating a compensation of these deficits. An initial study on experimental autoimmune encephalomyelitis (EAE) was performed in triple transgenic and control mice and the cell biology of oligodendrocytes obtained from the animals was studied in an in vitro myelination assay. Differences could be observed in these assays, which, however, did not achieve statistical significance, presumably because the majority of recombined LRP1-deficient cells has been replaced by non-recombined cells. Thus, the analysis of the role of LRP1 in EAE will require the induction of acute recombination in the context of the disease process. As LRP1 is necessary for the survival of OPCs in vivo, we assume that it will play an important role in myelin repair.
Association between apurinic/apyrimidinic endonuclease 1 rs1760944 T>G polymorphism and susceptibility of cancer: a meta-analysis involving 21764 subjects

Abstract Background: Previous case–control studies have suggested that apurinic/apyrimidinic endonuclease 1 (APE1) rs1760944 T>G polymorphism may be associated with cancer risk. Here, we carried out an updated meta-analysis to focus on the correlation between APE1 rs1760944 T>G locus and the risk of cancer. Methods: We used the crude odds ratios (ORs) with their 95% confidence intervals (CIs) to evaluate the possible relationship between the APE1 rs1760944 T>G polymorphism and cancer risk. Heterogeneity, publication bias and sensitivity analysis were also harnessed to check the potential bias of the present study. Results: Twenty-three independent studies involving 10166 cancer cases and 11598 controls were eligible for this pooled analysis. We found that APE1 rs1760944 T>G polymorphism decreased the risk of cancer in four genetic models (G vs. T: OR, 0.87; 95% CI, 0.83–0.92; P<0.001; GG vs. TT: OR, 0.77; 95% CI, 0.69–0.86; P<0.001; GG/TG vs. TT: OR, 0.83; 95% CI, 0.77–0.89, P<0.001 and GG vs. TT/TG: OR, 0.85; 95% CI, 0.80–0.92, P<0.001). Results of subgroup analyses also demonstrated that this single-nucleotide polymorphism (SNP) modified the risk among lung cancer, breast cancer, osteosarcoma, and Asians. Evidence of publication bias was found in the present study. When we treated the publication bias with ‘trim-and-fill’ method, the adjusted ORs and CIs were not significantly changed. Conclusion: In conclusion, current evidence highlights that the APE1 rs1760944 T>G polymorphism is a protective factor for cancer susceptibility. In the future, case–control studies with detailed risk factors are needed to confirm or refute our findings.
An Automatic Head Surface Temperature Extraction Method for Top-View Thermal Image with Individual Broiler

Surface temperature variation in a broiler’s head can be used as an indicator of its health status. Surface temperatures in the existing thermograph based animal health assessment studies were mostly obtained manually. 2185 thermal images, each of which had an individual broiler, were captured from 20 broilers. Where 15 broilers served as the experimental group, they were injected with 0.1mL of pasteurella inoculum. The rest, 5 broilers, served as the control group. An algorithm was developed to extract head surface temperature automatically from the top-view broiler thermal image. Adaptive K-means clustering and ellipse fitting were applied to locate the broiler’s head region. The maximum temperature inside the head region was extracted as the head surface temperature. The developed algorithm was tested in Matlab® (R2016a) and the testing results indicated that the head region in 92.77% of the broiler thermal images could be located correctly. The maximum error of the extracted head surface temperatures was not greater than 0.1 °C. Different trend features were observed in the smoothed head surface temperature time series of the broilers in experimental and control groups. Head surface temperature extracted by the presented algorithm lays a foundation for the development of an automatic system for febrile broiler identification.
QUERCETIN SUPPLEMENTATION PREVENTS CHANGES IN THE SEROTONIN AND CASPASE-3 IMMUNOREACTIVE CELLS OF THE JEJUNUM OF DIABETIC RATS.

BACKGROUND Serotonin (5-HT) is present in the epithelial enterochromaffin cells (EC), mast cells of the lamina propria and enteric neurons. The 5-HT is involved in regulating motility, secretion, gut sensation, immune system and inflammation. OBJECTIVE Evaluate the effects of diabetes and quercetin supplementation on serotoninergic cells and its cell loss by apoptosis in jejunal mucosa of streptozotocin-induced diabetic rats (STZ-rats). METHODS Twenty-four male Wistar rats were divided into four groups: normoglycemic (C), normoglycemic supplemented with 40 mg/day quercetin (Q), diabetic (D) and diabetic supplemented with 40 mg/day quercetin (DQ). After 120 days, the jejunum was collected and fixated in Zamboni's solution for 18 h. After obtaining cryosections, immunohistochemistry was performed to label 5-HT and caspase-3. Quantification of 5-HT and caspase-3 immunoreactive (IR) cells in the lamina propria, villi and crypts were performed. RESULTS The diabetic condition displayed an increase of the number of 5-HT-IR cells in villi and crypts, while decreased number of these cells was observed in lamina propria in the jejunum of STZ-rats. In the diabetic animals, an increased density of apoptotic cells in epithelial villi and crypts of the jejunum was observed, whereas a decreased number of caspase-3-IR cells was observed in lamina propria. Possibly, quercetin supplementation slightly suppressed the apoptosis phenomena in the epithelial villi and crypts of the STZ-rats, however the opposite effect was observed on the 5-HT-IR cells of the lamina propria. Quercetin supplementation on healthy animals promoted few changes of serotoninergic function and apoptotic stimuli. CONCLUSION These results suggest that quercetin supplementation mostly improved the serotonergic function affected by diabetes maybe due to antioxidant and anti-inflammatory properties of quercetin.
Clinical and genetic spectrum of an orphan disease MPAN: a series with new variants and a novel phenotype.

INTRODUCTION Pathogenic variations in C19orf12 are responsible for two allelic diseases: mitochondrial membrane protein-associated neurodegeneration (MPAN); and spastic paraplegia type 43 (SPG43). MPAN is an orphan disease, which presents with spasticity, dystonia, peripheral nerve involvement, and dementia. The pattern of iron accumulation on brain MRI may be a clue for the diagnosis of MPAN. SPG43, on the other hand, is characterised by progressive lower limb spasticity without brain iron accumulation. We here present clinical and genetic findings of MPAN patients with potentially pathogenic C19orf12 variants. MATERIALS AND METHODS Patients from 13 different families having progressive motor symptoms with irritative pyramidal signs and brain iron accumulation were screened for C19orf12 gene variants. RESULTS C19orf12 screening identified seven variants associated with MPAN in eight patients from seven families. We associated two pathogenic variants (c.24G > C; p.(Lys8Asn) and c.194G > A; p.(Gly65Glu)) with the MPAN phenotype for the first time. We also provided a genetic diagnosis for a patient with an atypical MPAN presentation. The variant c.32C > T; p.(Thr11Met), common to Turkish adult-onset MPAN patients, was also detected in two unrelated late-onset MPAN patients. CONCLUSIONS Genetic analysis along with thorough clinical analysis supported by radiological findings will aid the differential diagnosis of MPAN within the neurodegeneration with brain iron accumulation spectrum as well as other disorders including hereditary spastic paraplegia. Dystonia and parkinsonism may not be the leading clinical findings in MPAN patients, as these are absent in the atypical case. Finally, we emphasise that the existence of frameshifting variants may bias the age of onset toward childhood.
Artificial speech for intensive care unit (ICU) patients and laryngectomees

A method and prototype device to provide artificial speech for intensive care unit (leU) patients and laryngectomees is presented. The method assists these patients to produce natural sounding speech by "mouthing the words". A review of the current communication techniques for these patients is presented. The limitations of these techniques suggests that there is a need for a device that produces natural sounding speech (pitch variation and glottal sound source that resembles the actual glottal pulse generated by the vibrating vocal folds) and a device that is user friendly. As vocal folds only vibrate during vowel production, only vowel sounds are considered. Since pitch variation plays a major role in the naturalness of a person's voice, a number of alternative (automatic) pitch control tecbniques were explored. A unique pitch control technique utilising the changes in jaw height when a person "mouth the words" is presented. The electroglottographic (EGG) signal is used as the glottal sound source signal for this research as the properties of the EGG signal offers a number of advantages compared with other glottal sound source measurement techniques. A new glottal source model known as the twin-bar model, based on EGG measurements from normal volunteers, is also introduced. This model changes the shape of the glottal pulse based on a single parameter: pitch. Perceptual testing of the simulated voice using the twin-bar glottal model and two other well-known models on volunteers showed that the twin-bar model produces more natural sounding voice than the other two models. A new artificial speech system combining the automatic pitch control technique Gaw height) and the glottal sound source (twin-bar model) was constructed. It also includes a number of extra functions that would further improves the speech produced with this system. Existing technology on a laptop (e.g. serial port communication, bluetooth transceivers and USB port) is utilised for the construction of the prototype, with the laptop as the signal processing unit. The prototype was tested on a normal subject.
Photosynthesis in non‐foliar tissues: implications for yield

Summary Photosynthesis is currently a focus for crop improvement. The majority of this work has taken place and been assessed in leaves, and limited consideration has been given to the contribution that other green tissues make to whole‐plant carbon assimilation. The major focus of this review is to evaluate the impact of non‐foliar photosynthesis on carbon‐use efficiency and total assimilation. Here we appraise and summarize past and current literature on the substantial contribution of different photosynthetically active organs and tissues to productivity in a variety of different plant types, with an emphasis on fruit and cereal crops. Previous studies provide evidence that non‐leaf photosynthesis could be an unexploited potential target for crop improvement. We also briefly examine the role of stomata in non‐foliar tissues, gas exchange, maintenance of optimal temperatures and thus photosynthesis. In the final section, we discuss possible opportunities to manipulate these processes and provide evidence that Triticum aestivum (wheat) plants genetically manipulated to increase leaf photosynthesis also displayed higher rates of ear assimilation, which translated to increased grain yield. By understanding these processes, we can start to provide insights into manipulating non‐foliar photosynthesis and stomatal behaviour to identify novel targets for exploitation in continuing breeding programmes.
The novel aminoglycoside, ELX-02, permits CTNSW138X translational read-through and restores lysosomal cystine efflux in cystinosis

Background Cystinosis is a rare disorder caused by recessive mutations of the CTNS gene. Current therapy decreases cystine accumulation, thus slowing organ deterioration without reversing renal Fanconi syndrome or preventing eventual need for a kidney transplant.15-20% of cystinosis patients harbour at least one nonsense mutation in CTNS, leading to premature end of translation of the transcript. Aminoglycosides have been shown to permit translational read-through but have high toxicity level, especially in the kidney and inner ear. ELX-02, a modified aminoglycoside, retains it read-through ability without the toxicity. Methods and findings We ascertained the toxicity of ELX-02 in cells and in mice as well as the effect of ELX-02 on translational read-through of nonsense mutations in cystinotic mice and human cells. ELX-02 was not toxic in vitro or in vivo, and permitted read-through of nonsense mutations in cystinotic mice and human cells. Conclusions ELX-02 has translational read-through activity and produces a functional CTNS protein, as evidenced by reduced cystine accumulation. This reduction is comparable to cysteamine treatment. ELX-02 accumulates in the kidney but neither cytotoxicity nor nephrotoxicity was observed.