Miracle-dz/UniFAR-FTUs huggingface dataset:
Endovascular Treatment for Cerebral Aneurysms Using Stents

Endovascular stent placement combined with coil embolization is an established procedure for the treatment of intracranial aneurysms. We describe lesion outcome using stents for the treatment of intracranial aneurysms. Sixty-nine patients (30 females and 39 males; age range 12–78 years) treated with stent placement at Beijing Tiantan Hospital over a six-year period. Seventy-six cerebral aneurysms were encountered and 74 lesions were treated with 79 stents. Outcome was evaluated using the Glasgow Outcome Scale (GOS) score during a mean follow-up period of 32 months. Of the 74 stented aneurysms, 34 (45.9%) were located in the anterior circulation and 40 (54.1%) in the posterior circulation. Forty-eight (64.9%) aneurysms were small and 26 (35.1%) were large or giant. In 67 (90.5%) aneurysms embolization was completed by packing the aneurysm sac with platinum coils. In five small fusiform and four saccular aneurysms, the stent was used alone. Six procedure-related complications were observed and a cause of death in 7.2% of the patients. Follow-up angiography obtained at three months to one year in 68 (91.9%) aneurysms demonstrated complete occlusion in 43 aneurysms with a progressive thrombosis rate of 19.4% (six out of 31 aneurysms). Aneurysm recurrence occurred in four (5.8%) patients, three were treated with coil embolization and one with placement of another stent. Overall long-term outcome was good (GOS Score 4 or 5) in 92.8%, and fatal (GOS Score 1) in 7.2% of the patients. Intracranial stents can be a useful addition to coil embolization for complex broad-based aneurysms. Favorable overall long-term outcome can be achieved in 92.8% of patients when using endovascular stent placement techniques.
Mycobacterium ulcerans infection in a child from angola: diagnosis by direct detection and culture

Buruli ulcer, caused by Mycobacterium ulcerans, is a chronic ulcerative skin disease, found predominantly in central and west Africa and Australia. A boy of 2.5 years of age from Angola was admitted to our hospital with severe kwashiokor and a large ulcer with undermined edges on the left side of the thorax. Further examination revealed anaemia, hypoproteinaemia, bacterial superinfection of the ulcer and intestinal parasites. Histological analysis showed acid-fast bacilli and histopathological changes typical of Buruli ulcer. M. ulcerans was detected by PCR and culture. The patient was treated by surgical excision of diseased skin, followed by split-skin grafting. He also received antibiotic therapy (ciprofloxacin, clarithromycin, rifabutin, and dapsone). After six months, the child was discharged from hospital in good condition. This is the first published case of Buruli ulcer from Angola.
Long-term outcome in pulmonary arterial hypertension patients treated with subcutaneous treprostinil

Pulmonary arterial hypertension (PAH) is fatal if untreated. Intravenous epoprostenol improves exercise capacity and haemodynamics in PAH, and increases survival in idiopathic PAH (IPAH). To evaluate the effects of subcutaneous (SC) treprostinil, a longer-acting prostacyclin analogue, followed by the addition of other PAH therapies if needed, 860 PAH patients treated with SC treprostinil for up to 4 yrs were followed. Survival is reported as Kaplan–Meier estimates. For 332 IPAH patients with baseline haemodynamics, observed survival is also compared with predicted survival using the National Institute of Health formula. Out of the 860 patients, 199 (23%) discontinued due to adverse events, 136 (16%) died, 117 (14%) discontinued due to deterioration, 29 (3%) withdrew consent and 11 (1%) underwent transplantation. In total, 97 patients (11%) switched from SC treprostinil to an alternative prostacyclin analogue; bosentan was added in 105 patients (12%) and sildenafil in 25 (3%). In conclusion, survival was 87–68% over 1–4 yrs for all 860 patients and 88–70% over 1–4 yrs with subcutaneous treprostinil monotherapy. For the idiopathic pulmonary arterial hypertension subset with baseline haemodynamics (n = 332), survival was 91–72% over 1–4 yrs. In contrast, predicted survival was 69–38% over 1–4 yrs. The safety profile for long-term subcutaneous treprostinil was consistent with previous short-term trials with no unexpected adverse events.
Development and Biological Analysis of a Neural Network Based Genomic Compression System

The advent of Next Generation Sequencing (NGS) technologies has resulted in a barrage of genomic data that is now available to the scientific community. This data contains information that is driving fields such as precision medicine and pharmacogenomics, where clinicians use a patient’s genetics in order to develop custom treatments. However, genomic data is immense in size, which makes it extremely costly to store, transport and process. A genomic compression system which takes advantage of intrinsic biological patterns can help reduce the costs associated with this data while also identifying important biological patterns. In this project, we aim to create a compression system which uses unsupervised neural networks to compress genomic data. The complete compression suite, GenComp, is compared to existing genomic data compression methods. The results are then analyzed to discover new biological features of genomic data. Testing showed that GenComp achieves at least 40 times more compression than existing variant compression solutions, while providing comparable decoding times in most applications. GenComp also provides some insight into genetic patterns, which has significant potential to aid in the fields of pharmacogenomics and precision medicine. Our results demonstrate that neural networks can be used to significantly compress genomic data while also assisting in better understanding genetic biology.
B-Cell Lymphoma Induction by Akv Murine Leukemia Viruses Harboring One or Both Copies of the Tandem Repeat in the U3 Enhancer

ABSTRACT Akv is an endogenous, ecotropic murine leukemia virus (MuLV) of the AKR strain. It has served as a prototype nonpathogenic or weakly pathogenic reference virus for studies of closely related potent lymphomagenic viruses such as the T-lymphomagenic SL3-3. We here report that Akv and an Akv mutant (Akv1-99) with only one copy of the 99-bp transcriptional enhancer induce malignant lymphomas with nearly 100% incidence and mean latency periods of 12 months after injection into newborn NMRI mice. Molecular analysis of tumor DNA showed that the majority of the tumors were of the B-cell type. Sequence analysis of proviral transcriptional enhancers in DNA of B-cell lymphomas revealed conservation of the enhancer sequence, as well as a lack of sequence duplications of the Akv1-99 variant, while the repeat copy number in Akv was subject to fluctuations. In support of a B-cell specificity of the Akv enhancer, a murine plasmacytoma cell line was found to sustain three- to fivefold-higher transient transcriptional activity upon the Akv and Akv1-99 enhancers than upon the enhancer of the T-lymphomagenic SL3-3 MuLV. Thus, the overall picture is that Akv MuLV possesses a B- lymphomagenic potential and that the second copy of the 99-bp sequence seems to be of minor importance for this potential. However, in one animal the lymphomas induced by Akv1-99 were of the T-cell type. Among the 24 tumors analyzed only this one harbored a clonal proviral integration in the c-myc locus. This provirus had undergone a duplication of a 113-bp sequence of the enhancer region, partly overlapping with the 99-bp repeat of Akv, as well as a few single nucleotide alterations within and outside the repeats. Taken together with previous studies, our results suggest that T- versus B-lymphomagenic specificity of the enhancer is governed by more than one nucleotide difference and that alterations in binding sites for transcription factors of the AML1 and nuclear-factor-1 families may contribute to this specificity.
Interplay of Biomechanical Constraints and Kinematic Strategies in Selecting Arm Postures

Abstract In this study, the authors examined the interplay between biomechanics and control strategies in the resolution of excess degrees of freedom at the joint level. Seven participants made aimed arm movements from 30 starting points and several starting postures to targets. Final arm postures for movements to a target exhibited substantial joint angle variation. Through regression modeling and by comparing observed final arm postures with biomechanically plausible postures, the authors identified 3 kinematic strategies: (a) Maintain deviations from the average angle at the starting point to the joint's final posture; (b) make torso rotations that are a fixed proportion of shoulder rotations; and (c) adopt a characteristic combination of 4 wrist-positioning approaches. The results demonstrated that kinematic strategies can account for substantial variance in final arm postures, if one takes into account 2 types of individual differences—those that arise inevitably from biomechanical constraints and those that reflect choices in movement strategy.
Four-Cantilever Trace Explosive Sensors with Dual SAMs Functionalized for Specific-Sensing Improvement and Nonspecific-Adsorption Depression

A top-down formed four-cantilever micro-sensor structure is equipped with self-assembled dual monolayers on the surfaces for specific detection to trace explosive and depression of environmental cross-talk. Sensor design of the four-lever scheme, micro-fabrication, surface modification of the dual monolayers and experimental results for ultra-sensitive detection of explosive are sequentially related. The testing results confirm that the sensor can detect TNT at the resolution level of several tens of ppt and can effectively depress the influences from environmental temperature and humidity change.
Prevalence of Vancomycin Resistant Enterococci in Hilla City, Iraq

To identify the prevalence of enterococci species in hospitalized patients with urinary tract infection in Hilla City, to determine their susceptibilities to vancomycin and other antibiotics. Materials and methods: Two hundred urine samples were obtained from patients who were admitted to three main hospitals in Hilla city during a period of three months (from November 2010 to February 2011). After identification of enterococcal species by biochemical tests, the antibiotic susceptibility of isolates was determined by standard disk diffusion test according to CLSI. MIC tests for vancomycin and teicoplanin were also carried out for VRE isolates by methods of agar-dilution and HiComb test. Results: This study revealed that out of 200 samples, Enterococcous species was isolated only from 7 samples, which were identified to genus level. 5/7 isolates were catheter-related infection. According to susceptibility data obtained, Five isolates were resistant to vancomycin (71.43%) with an MIC ranging from 8-32 μg/ml but they were sensitive to teicoplanin (MIC < 0.25 μg/ml), thus exhibited VanB phenotype, and the majority of isolates (86%) showed multi-drug resistance pattern. All isolates were highly resistant (85.72%) for each of chloramphenicol, rifampicin and erythromycin. On the other hand, 71.43% of isolates were resistant to each of penicillin and tetracycline while they were moderately resistant to ampicillin (57.14%) and ciprofloxacin (42.86%), in comparison to relatively low resistance to nitrofurantoin 14.29%. Conclusion: Finding of this study shows an emergence of VRE along with increased rate of multi- drug resistant enterococci in the area of the study. قارعلا /ةلحلا ةنيدم يف نيسياموكنافلا داضمل ةمواقملا ةيوعملا تايحبسملا راشتنا لدعم
A 12-week, randomized, double-blind, placebo-controlled study evaluating the effect of eszopiclone 2 mg on sleep/wake function in older adults with primary and comorbid insomnia.

BACKGROUND Longer-term pharmacologic studies for insomnia in older individuals are sparse. OBJECTIVE To evaluate the efficacy and safety of 12 weeks of nightly eszopiclone in elderly outpatients with insomnia. METHODS Participants (65-85 years) met DSM-IV-TR criteria for insomnia with total sleep times (TST) < or = 6 h, and wake time after sleep onset (WASO) > or = 45 min. Participants were randomized to 12 weeks of eszopiclone 2 mg (n = 194) or placebo (n = 194), followed by a 2-week single-blind placebo run-out. Subject-reported measures of sleep (sTST, sleep latency [sSL], sWASO) and daytime function (alertness, concentration, wellbeing, ability to function) were assessed. AEs were monitored. RESULTS Subjects treated with 2 mg eszopiclone slept longer at night on average and at every individual time point compared to baseline than placebo subjects, as measured by TST over the 12-week double-blind period (P < 0.0001). Mean sTST over the double-blind period for eszopiclone-treated subjects was 360.08 min compared to 297.86 min at baseline, a mean change of 63.24 min. Over the double-blind period, eszopiclone-treated subjects also experienced a significantly greater improvement in sSL compared to placebo, with a mean decrease of 24.62 min versus a mean decrease of 19.92 min, respectively (P = 0.0014). Eszopiclone subjects also experienced a significantly greater decrease in WASO (mean decrease of 36.4 min) compared to placebo subjects (decrease of 14.8 min) (P < 0.0001). Post-discontinuation, sleep parameters were statistically improved versus baseline for eszopiclone (P-values < or = 0.01), indicating no rebound. The most common AEs (> or = 5%) were headache (eszopiclone 13.9%, placebo 12.4%), unpleasant taste (12.4%, 1.5%), and nasopharyngitis (5.7%, 6.2%). CONCLUSION In this Phase IV trial of older adults with insomnia, eszopiclone significantly improved patient-reported sleep and daytime function relative to placebo. Improvements occurred within the first week and were maintained for 3 months, with no evidence of rebound insomnia following discontinuation. The 12 weeks of treatment were well tolerated. CLINICAL TRIAL INFORMATION A Long-Term Safety and Efficacy Study of Eszopiclone in Elderly Subjects With Primary Chronic Insomnia; Registration #NCT00386334; URL - http://www.clinicaltrials. gov/ct2/show/NCT00386334? term=eszopiclone&rank=24
Exercise-based cardiac rehabilitation improves endothelial function assessed by flow-mediated dilation but not by pulse amplitude tonometry*

We aimed to investigate the effect of exercise on endothelium-dependent vasodilator function assessed simultaneously in the brachial artery and in the distal arterial bed by flow-mediated dilation and the pulse amplitude tonometry method, respectively, in coronary artery disease patients. The study included 146 patients with stable coronary artery disease (123 men, mean age 62 ± 9 years) who participated in the Cardiac Rehabilitation and Genetics of Exercise performance study. All patients completed a 12-week supervised cardiac rehabilitation programme (three sessions per week at an intensity of 80% of the heart rate reserve). At baseline and upon completion of the training, we measured brachial artery diameters by means of ultrasound scanning (linear array transducer of 12 MHz) and simultaneously assessed pulse amplitudes in the fingertip using a pulse amplitude tonometry device both at rest and after reactive hyperaemia induced by a 5-min forearm cuff occlusion. Peak oxygen uptake significantly increased (+22%; p < 0.0001) and flow-mediated dilation improved from 10.0% to 13.1% (+37%; p < 0.0001), whereas the reactive hyperaemia index of the pulse amplitude tonometry method remained unchanged (p = 0.47) following exercise-based cardiac rehabilitation. However, the basal digital pulse amplitude (+58%; p < 0.001) increased as a result of training, as did the digital pulse amplitude after reactive hyperaemia (+22%; p < 0.05). Exercise-based cardiac rehabilitation is associated with an improvement in endothelial function, as can be measured by flow-mediated dilation but not by the reactive hyperaemia index of the pulse amplitude tonometry method.
[Expression of laminin 5 in rat skin wounds regulated by recombinant thymosin beta4].

OBJECTIVE To explore the mechanism of recombinant thymosin beta4 (Tbeta4) accelerating skin wound healing in rats by regulating laminin 5 expression. METHODS Two full thickness 8 mm punch wounds were made at the costovertebral angle on dorsal surface of each adult male rats weighing 200-250 g. Sixty rats were randomized into the control group (n = 15) and the experimental group (n = 45), which was subdivided into low, medium and high dose groups (n = 15). Tbeta4 was applied topically at 2, 6, 18 microg in 50 microL PBS for every 12 hours after model making in the experimental group. The identical amounts of phosphate buffered saline was applied in the control group. Wound healing was observed after model making and immunohistochemical observation was conducted 2, 4 and 7 days after operation. RESULTS Seven days after operation, wound contracted obviously and most of the wounds connected well with the margin. In the control group, low dose group, medium dose group and high dose group, the wound healing rate were 7.67% +/- 5.46%, 29.01% +/- 7.43%, 26.54% +/- 11.49% and 10.39% +/- 3.96% respectively 2 days after operation; 28.16% +/- 13.76%, 37.99% +/- 13.05%, 42.00% +/- 9.56% and 39.58% +/- 12.74% respectively 4 days after operation; 59.08% +/- 19.02%, 64.15% +/- 17.92%, 77.39% +/- 8.45% and 69.78% +/- 8.45% respectively 7 days after operation. At 2 days after operation, significant differences were notified in healing rats between 3 sub-experimental groups and the control group (P < 0.05). Immunohistochemistry staining showed that there was a little more positive expression of laminin 5 2 days after operation that beneficial to promote the proliferation and differentiation of cell in every group, including positive cells and ECM. But in medium group there was fewer expression, only at the borderline and bottom of the wound, while the expression significantly increased 4 days after operation (P < 0.05) and there was a relative high expression 7 days after operation (P < 0.01). CONCLUSION Tbeta4 can inhibit the expression of laminin 5 early, and then up-regulate laminin 5 expression to moderate the reformation of ECM, promote the migration of epidemic cell and accelerate skin wound healing.
The Tissue Is the Issue: Personalized Medicine for Non-Small Cell Lung Cancer

The development of molecular markers is crucial for improving therapy in advanced non-small cell lung cancer (NSCLC). Here, we review perspectives of biomarker-driven personalized therapy in NSCLC, with particular reference to the detection of EML4-ALK gene rearrangements. Different molecular assays, validation, and clinical application to different types of tissue specimens are discussed. Clin Cancer Res; 16(20); 4909–11. ©2010 AACR.
Control strategies to prevent total hip replacement-related infections: a systematic review and mixed treatment comparison

Objective To synthesise the available evidence and estimate the comparative efficacy of control strategies to prevent total hip replacement (THR)-related surgical site infections (SSIs) using a mixed treatment comparison. Design Systematic review and mixed treatment comparison. Setting Hospital and other healthcare settings. Participants Patients undergoing THR. Primary and secondary outcome measures The number of THR-related SSIs occurring following the surgical operation. Results 12 studies involving 123 788 THRs and 9 infection control strategies were identified. The strategy of ‘systemic antibiotics+antibiotic-impregnated cement+conventional ventilation’ significantly reduced the risk of THR-related SSI compared with the referent strategy (no systemic antibiotics+plain cement+conventional ventilation), OR 0.13 (95% credible interval (CrI) 0.03–0.35), and had the highest probability (47–64%) and highest median rank of being the most effective strategy. There was some evidence to suggest that ‘systemic antibiotics+antibiotic-impregnated cement+laminar airflow’ could potentially increase infection risk compared with ‘systemic antibiotics+antibiotic-impregnated cement+conventional ventilation’, 1.96 (95% CrI 0.52–5.37). There was no high-quality evidence that antibiotic-impregnated cement without systemic antibiotic prophylaxis was effective in reducing infection compared with plain cement with systemic antibiotics, 1.28 (95% CrI 0.38–3.38). Conclusions We found no convincing evidence in favour of the use of laminar airflow over conventional ventilation for prevention of THR-related SSIs, yet laminar airflow is costly and widely used. Antibiotic-impregnated cement without systemic antibiotics may not be effective in reducing THR-related SSIs. The combination with the highest confidence for reducing SSIs was ‘systemic antibiotics+antibiotic-impregnated cement+conventional ventilation’. Our evidence synthesis underscores the need to review current guidelines based on the available evidence, and to conduct further high-quality double-blind randomised controlled trials to better inform the current clinical guidelines and practice for prevention of THR-related SSIs.
Professional Skills Requirements of IT Professional Practice: Australian IT Graduate Perspectives

This paper examines the professional work experiences of recent Australian Information Technology graduates. The aim is to understand and describe the typical professional skill requirements of IT professional practice, identify graduate perceptions of the practical relevance of their university courses to the professional skill requirements of their work and to identify the challenges that graduates face when they commence employment. The research findings, collected from new IT graduates, will provide IT faculties in universities with evidence to support the preparation of graduates for professional practice through the development of appropriate curricula. Similarly, the evidence will help IT employers develop transition strategies for new IT graduates.
Can young adult patients with proteinuric IgA nephropathy perform physical exercise?

BACKGROUND It is not known whether physical exercise increases daily proteinuria in patients with proteinuric nephropathies, thus accelerating progression of the renal lesion. This study evaluates the acute effects of physical exercise on proteinuria in young adults with immunoglobulin A (IgA) nephropathy. METHODS Changes induced by intense physical exercise on quantitative and qualitative proteinuria were evaluated in basal conditions and after 10 days of ramipril therapy in 10 patients with IgA nephropathy, normal glomerular filtration rate (GFR), proteinuria between 0.8 and 1.49 g/24 h, and "glomerular" microhematuria before and after the end of a maximal treadmill Bruce test (B-test). The basal study also was performed in 10 age- and sex-matched healthy volunteers. RESULTS At rest, GFR averaged 141 +/- 23 mL/min; it increased by 16.3% +/- 3.3% (P < 0.005) and 7.1% +/- 1.6% at 60 and 120 minutes after the B-test, respectively. At rest, GFR-corrected proteinuria averaged protein of 0.76 +/- 0.21 mg/min/100 mL GFR; it increased to 1.55 +/- 0.28 mg/min/100 mL GFR after 60 minutes (P < 0.001) and declined to 0.60 +/- 0.11 mg/min/100 mL GFR at 120 minutes after the end of the B-test. The pattern of urinary proteins remained unchanged, as did microhematuria. Daily proteinuria was not different from the basal value on the day of the B-test. After ramipril therapy, patients showed a reduction in GFR, but no change in daily GFR-corrected proteinuria, pattern of urinary proteins, or hematuria. CONCLUSION The increase in proteinuria after exercise in our patients is significant and is not prevented by ramipril therapy, but lasts less than 120 minutes. Therefore, it cannot modify daily proteinuria. Thus, these data do not support the need to reduce acute physical activity in patients with nonnephrotic renal diseases.