Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression analysis of dreb subfamily genes in leaves and roots of Coffea canephora conilon subjected to drought

Coffea canephora is one of the most economically important coffee species and in Brazil, Conilon being the most widely cultivated plant of this species. Abiotic stresses such as temperature variations and drought periods are factors that significantly affect their production and tend to worsen with globally recognized climate changes. Rationale: In an attempt to understand the molecular responses of coffee plants in water deficit conditions, recent studies have identified candidate genes as CcDREB1D (Dehydration Responsive Element Binding Protein). This gene showed increased expression in response to drought in the leaves of clone 14 (drought tolerant) in relation to the clone 22 (drought susceptible) of C canephora Conilon (Marraccini et al., 2012). Methods: The main objectives of this work were (1) to identify DREB genes and their subgroups (SGs) of C. canephora, and (2) to analyze their expression by reverse transcription-quantitative PCR in leaves and roots of drought-tolerant (14, 73 and 120) and drought-susceptible (22) clones of C canephora Conilon submitted (or not) to drought stress. Results: Among the four subgroups (I-IV) of coffee DREB genes (Alves, 2015), we identified several DREB genes showing differential expression profiles in leaves in response to drought. For several of them, differential expression profiles under drought were observed between drought-tolerant and drought-susceptible clones of C. canephora. This was the case for Cc05_g06840 (SG-I) and Cc08_g13960 (SG-III) DREB that were highly up-regulated by drought in leaves of drought-tolerant clones 14 and 73, but not in those of the drought-susceptible clone 22. Conclusions and Perspectives: The fact that several DREB genes were differentially expressed under drought between drought-tolerant and drought-susceptible clones of C. canephora highly suggests that these genes play a key role in response of coffee plants to this abiotic stress. (Texte integral)
A RISC-V Based Coprocessor Accelerator Technology Research for Convolution Neural Networks

The advancements of neural networks have significantly improved the accuracy of a variety of intelligent applications, such as image processing, voice recognition and so on. However, one of the challenges is to accelerate the speed of inference with the networks designed to be deeper. In this paper, we first realize the algorithm involved in software. Then, combined with the expanded characteristic of RISC-V architecture, the acceleration of convolution operation taking up the largest proportion of computation in CNN is realized by coprocessor expansion mode on open-source Hummingbird E203 processor. Through tests, convolution coprocessor improves the performance of CNN while ensuring the accuracy in function. Finally, the Cifar-10 image classification, a common benchmark in machine learning, is used to verify the feasibility in functions and comparatively analysis the pure software-based and coprocessor-based implementations.
A record of hypertrophy of the tragus of great fruit-eating bat Artibeus lituratus (Chiroptera: Phyllostomidae)

In this work, we report and describe evidence of an anomaly in the tragus of an individual of Artibeus lituratus, captured in the Central Andes of Colombia. During field work in the Department of Caldas, municipality of Aranzazu, one individual of A. lituratus with unusual tragus form was trapped. This individual had a tragus with cylindrical shape and asymmetry. The individual was collected and deposited at the Museum of Natural History of the University of Caldas (MHN-UCa). A literature review was conducted to find similar cases using searching engines, but no previous reports of the anomaly were find. Furthermore, we reviewed the tragus of vouchers at the MHN-UCa and none of these had similar malformations. We concluded that this anomaly has not previously registered in Chiroptera.
Age effects on the development of stimulus over-selectivity are mediated by cognitive flexibility and selective attention

Stimulus over-selectivity is said to have occurred when only a limited subset of the total number of stimuli present during discrimination learning controls behavior, thus, restricting learning about the range, breadth, or all features of a stimulus. The current study investigated over-selectivity of 100 typically developing children, aged 3–7 (mean = 65.50 ± 17.31 SD months), using a visual discrimination task. Developmental trends in over-selectivity and their relationship to some cognitive variables (i.e., selective attention, sustained attention, and cognitive flexibility) were the target. Over-selectivity decreased with age, but this effect was mediated by the development of cognitive flexibility. Over-selectivity increased when a distractor task was introduced, which was not mediated by the other cognitive variables under investigation. The current results assist in the establishment of the theoretical underpinnings of over-selectivity by offering evidence of its underlying determinants and relating these to developmental trends.
Examination of eukaryotic chaperonin-mediated nascent chain folding in the cytosol: a photocrosslinking approach

Examination of Eukaryotic Chaperonin-Mediated Nascent Chain Folding in the Cytosol: A Photocrosslinking Approach. (August 2003) Stephanie Anne Etchells, B.Sc.H. , Queen’s University Chair of Advisory Committee: Dr. Arthur E. Johnson TRiC (TCP-1 ring complex), a type II chaperonin, facilitates protein folding, and we previously showed that TRiC crosslinks to ribosome-bound actin and luciferase nascent chains. Here, it was found that actin and luciferase nascent chains were adjacent to more than one TRiC subunit at different stages of translation. Six and seven out of the eight TRiC subunits were photocrosslinked to the luciferase and actin nascent chains, respectively. Actin nascent chains with widely-spaced, site-specific probe locations were adjacent to the same three TRiC subunits (α, β and ε) at different stages of translation. The exposure of other TRiC subunits to nascent chains varied with the length and identity of the nascent chain. In addition, the presence or absence of ATP influences the photocrosslinking yields. This suggests that ATP alters the conformation of the subunits and/or their affinity for the nascent chain. Photocrosslinking also revealed that TRiC is in close proximity to the exit site of the ribosomal tunnel, presumably to create a protected folding environment for the nascent chain.
Diagnosis and Therapeutic Management of Babesia gibsoni Infection in Dog

Canine babesiosis is a life threatening haemoprotozoan disease mainly caused by Babesia canis or Babesia gibsoni. Amongst the various prevalent canine vector borne diseases, babesiosis is a common disease caused by intra-erythrocyte protozoa B. gibsoni is a small piroplasm (1.0–2.5μm), that occurs as a single ring form or pyriform bodies within the erythrocyte (Conrad et al., 1991; Birkenheuer et al., 1999). Dogs get infection when they are inoculated with sporozoites of B. gibsoni present in tick saliva. After inoculation, Babesia sporozoites invade erythrocytes where they multiply International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 8 (2020) Journal homepage: http://www.ijcmas. com
A Novel Lentiviral Vector Model to Investigate the Mechanism of Insertional Mutagenesis by Aberrant Splicing

Retrovirus vector-mediated insertional mutagenesis (IM) can lead to serious cancerous risks in gene therapy. Although multiple cases of IM-induced malignancies have been reported in clinical trials with gammaretroviral vectors, no evidence for malignancies has been reported with HIV-derived lentiviral vectors (LVs). However, the 2010 clinical trial for s-thalassaemia had reported the clonal dominance of erythroblasts in one patient. This was caused by the LV integration into the HMGA2 gene locus and the up-regulation of HMGA2 protein by the formation of host-vector chimeric transcripts (Cavazzana-Calvo et al., 2010). The observed pattern of splicing in the HMGA2 locus, splice-in, is splicing from a host splice donor to a vector splice acceptor. Considering that this literature is the first and only evidence that implies mutagenicity of LVs, We thought that formation of splice-in fusion transcripts could be a key factor to contribute potential LV-mediated IM. Therefore, we set our aim of investigating the mechanism of splice-in caused by vector integration by designing a novel LV model that can induce splice-in fusion transcripts, and by applying to an in vitro IM assay previously established by our group (Bokhoven et al., 2009). The IM assay utilise IL-3 dependent Bcl15 cell transformation into IL-3 independent, which can be analysed for the transformation mechanism via LV integration. Phenotypic analysis of the isolated mutant clone (the IL-3I clone) suggested secretion of autocrine factors and the cause of which was interrogated by molecular analysis. Additionally, the IL-3I clone and two bulk populations from different steps of an IM assay were subjected to RNA sequencing (RNA-Seq) to compare the isolated host genes and investigate chimeric mRNAs. Importantly, RNA-Seq identified the Angpt1 fusion transcript but in the splice-out form using a cryptic vector SD as a possible cause for cell transformation of the IL-3I clone. This gene locus has Retroviral Tagged Cancer Gene Database (RTCGD) hits and was also detected by LM-PCR. A variety of splice sites were used in the detected possible fusion mRNAs. To confirm, Angpt1 loci is the major cause of IL-3 independence and the pattern of splice sites use depend integration site; further analysis is required and discussed in the final chapter.
Analysis Price, Perception of Quality, and Promotion with Intervening Brand Trust Toward Purchase Intention Honda Vario 150CC (Case Study at PT Wahana Makmur Sejati)

This research was conducted to analyze the effect of Price, Perceived Quality, and Promotion on Purchase Intention on Honda Vario 150CC products with Intervening Brand Trust at PT Wahawa Makmur Sejati. The data used in this study is questionnaire data taken from 200 respondents using convenience sampling. Meanwhile, the data analysis was performed using quantitative statistics using Structural Equation Modeling (SEM) assisted by LISREL version 9.2 software. The results showed that Price, Perceiveds of Quality and Promotion had a significant effect on Brand Trust, and also Brand Trust had a significant effect on Consumer Purchase Intention. In the research results, the direct and indirect relationship, both Price, Perceived of Quality and Promotion, directly have a significant effect on the purchase intention of Vario 150CC. However, the variable Price and Perceived Quality indirectly through Brand Trust does not have a significant effect on consumer Purchase Intention. The results of this research can be used as a reference both academically and practically in implementation management by PT WAHANA MAKMUR SEJATI.
LIVRO DIDÁTICO PARA O ENSINO DE LÍNGUA INGLESA NO ENSINO FUNDAMENTAL I: REFLEXÕES SOBRE CONTRIBUIÇÕES DIDÁTICAS PARA A FORMAÇÃO CIDADÃ

Este artigo propoe-se a analisar o livro didatico Super Stars 1 da editora Oxford e seu respectivo manual do professor, desenvolvido para alunos brasileiros do 1° ano do Ensino Fundamental e utilizado para as aulas regulares de Lingua Inglesa em uma Rede Municipal de Ensino do Estado de Sao Paulo. No âmbito da escola publica municipal, a analise, insere-se em uma abordagem qualitativa de pesquisa de carater descritivo e critico (GODOY, 1995) e focara em criterios delineados a partir dos principais documentos nacionais norteadores para o Ensino Fundamental I e para o ensino de lingua adicional, como a Base Nacional Comum Curricular (BRASIL, 2018), os Parâmetros Curriculares Nacionais para o EFI (BRASIL, 1997) e para lingua estrangeira moderna (BRASIL, 1998), alem de dialogar com um suporte teorico que embasa o aprendizado de lingua adicional em uma perspectiva socio-historica-discursiva atraves do desenvolvimento dos multiletramentos, materializados nos diferentes generos discursivos (BAKHTIN, 2006, 2011; KUMARAVADIVELU, 2003; ROCHA, 2007, 2010; ROJO, 2009, 2012; VYGOTSKY, 1991, 2001a, 2001b). Com este trabalho, foi possivel concluir que, ao apresentar a lingua por meio de vocabulario e frases isoladas, o livro didatico, em questao, pouco se envolve em situacoes comunicativas voltadas para a funcao social do Ingles. Assim, surge a necessidade de investimento em pesquisas sobre a elaboracao de materiais didaticos de Lingua Inglesa, voltados para os anos iniciais do Ensino Fundamental, que sejam condizentes com os objetivos da educacao publica nacional, levando em consideracao os interesses da comunidade local e as especificidades para essa faixa-etaria, devido a crescente expansao do ensino do idioma nas escolas publicas.
Construction and Application of Big Data System in Automobile Intelligent Factory

Under the background of intelligent manufacturing, the application of industrial big data is the key to improve the utilization efficiency of manufacturing resources. Starting from the purpose of big data application and how to replace the efficiency of enterprise resource utilization, aiming at the construction of intelligent and transparent big data system in automobile factory, this paper combed the concept of big data system construction, discussed the construction ideas of big data system, constructed the big data system framework suitable for discrete manufacturing enterprises, and gave the methods of data acquisition, transmission, storage, processing and Analysis of the specific program. For big data applications, taking the enterprise intelligent operation cockpit, intelligent electronic process system and enterprise epidemic prevention and health cloud system as examples, the scene and application effect of big data application are demonstrated. The construction and application of big data system in intelligent and transparent automobile factory provides a reference model for further promoting intelligent manufacturing driven by big data.
Recent advances of triboelectric nanogenerator based applications in biomedical systems

Funding information Chinese University of Hong Kong, Grant/ Award Number: 4055086; Hong Kong Polytechnic University, Grant/Award Number: P0030234; Research Grants Council, University Grants Committee, Grant/Award Number: 24206919 Abstract Triboelectric nanogenerators (TENGs) are a promising power and sensing solution for biomedical applications attributed to the high electrical output and sensitivity with low-cost and widely available materials. Here, the recent developments in TENG-based biomedical applications with various structures and functionalities are discussed and summarized. Wearable TENGs perform well in motion and respiratory monitoring with excellent flexibility and low bio-risks. Implantable TENGs enable interactions with the cardio-cerebral vascular system and nervous system to monitor and treat different diseases with good biodegradability. Furthermore, explorations of TENGs in in vitro systems, including drug delivery, cellular proliferation and differentiation, are reviewed. Perspectives on the biomedical applications of TENGs are proposed based on investigation in this area in the past two decades. With continuous efforts in miniaturization and optimization of robustness, the TENG-based self-powered biomedical systems will definitely play an important role in the healthcare market in the future.
High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci

The practical use of molecular markers is facilitated by cost-effective detection techniques. In this work, wheat insertion site-based polymorphisms (ISBP) markers were set up for genotyping using high-resolution melting analysis (HRM). Polymorphic HRM-ISBP assays were developed for wheat chromosomes 4A and 3B and used for wheat variability assessment. The marker sequences were mapped against the wheat genome reference sequence, targeting interesting genes. Those genes were located within or in proximity to previously described quantitative trait loci (QTL) or meta-quantitative trait loci (MQTL) for drought and heat stress tolerance, and also yield and yield related traits. Eighteen of the markers used tagged drought related genes and, interestingly, eight of the genes were differentially expressed under different abiotic stress conditions. These results confirmed HRM as a cost-effective and efficient tool for wheat breeding programs.
Estimating disperser abundance using open population models that incorporate data from continuous detection passive integrated transponder arrays

Autonomous passive integrated transponder (PIT) tag antenna systems continuously detect individually marked organisms at one or more fixed points over long time periods. Estimating abundance using data from autonomous antennas can be challenging because these systems do not detect unmarked individuals. Here we pair PIT antenna data from a tributary with mark–recapture sampling data in a mainstem river to estimate the number of fish moving from the mainstem to the tributary. We then use our model to estimate abundance of non-native rainbow trout (Oncorhynchus mykiss) that move from the Colorado River to the Little Colorado River, the latter of which is important spawning and rearing habitat for federally endangered humpback chub (Gila cypha). We estimate that 226 rainbow trout (95% confidence interval: 127–370) entered the Little Colorado River from October 2013 to April 2014. We discuss the challenges of incorporating detections from autonomous PIT antenna systems into mark–recapture population models, particularly in regards to using information about spatial location to estimate movement and detection probabilities.
Pansharpening by Complementing Compressed Sensing with Spectral Correction

Pansharpening (PS) is a process used to generate high-resolution multispectral (MS) images from high-spatial-resolution panchromatic (PAN) and high-spectral-resolution multispectral images. In this paper, we propose a method for pansharpening by focusing on a compressed sensing (CS) technique. The spectral reproducibility of the CS technique is high due to its image reproducibility, but the reproduced image is blurry. Although methods of complementing this incomplete reproduction have been proposed, it is known that the existing method may cause ringing artifacts. On the other hand, component substitution is another technique used for pansharpening. It is expected that the spatial resolution of the images generated by this technique will be as high as that of the high-resolution PAN image, because the technique uses the corrected intensity calculated from the PAN image. Based on these facts, the proposed method fuses the intensity obtained by the component substitution method and the intensity obtained by the CS technique to move the spatial resolution of the reproduced image close to that of the PAN image while reducing the spectral distortion. Experimental results showed that the proposed method can reduce spectral distortion and maintain spatial resolution better than the existing methods.
Seedling emergence and activity of some antioxidant enzymes of canola (Brassica napus) can be increased by seed priming

SUMMARY Late sowing causes substantial yield reduction in canola. This yield reduction is principally due to lower emergence and poor crop establishment because of prevailing low temperatures. A field experiment was conducted using five sowing dates over two growing seasons (2014/15 and 2015/16). The effect of two seed-priming strategies (hydropriming and osmopriming with zinc sulphate, ZnSO4) was measured in three canola cultivars (Okapi, Zarfam and Talayeh). Results showed that both seed-priming treatments were effective in improving emergence percentage (EP) of canola cultivars. The highest EP occurred in the earlier sowing in both years. On 5 September, EP for the hydropriming, osmopriming and no-primed treatments were 89, 93 and 73% in Okapi, 83, 89 and 69% in Zarfam, and 81, 89 and 72% in Talayeh, respectively (data averaged across both years). Delay in sowing date compared with 5 September caused a sharp reduction in EP in primed seed and control conditions. Maximum seedling emergence rate and coefficient of uniformity of emergence were seen in early sowing for Okapi cultivar with ZnSO4 osmopriming. Experimental evidence showed that in canola cultivars the activities of superoxide dismutase, peroxidase and catalase were higher in the leaves of plants from seed primed with ZnSO4 compared with seed from hydroprimed plants. Cultivar analysis of total antioxidant content activities indicated, the maximum increase was observed in the Okapi and Talayeh cultivars.
Comparisons of Spontaneous Mutation Rates and Estimates of Numbers of Cell Divisions in the Germlines of Three Metazoans.

Germline mutation rates measured per generation per locus in three species of Metazoa are used to address questions of the constancy of mutation rates per generation, per year and per germcell division. Null mutation rates per generation are: 3.6 x 10-6 for human X-linked disease, 8.9 x 10-6 for Specific Locus Test (SLT) in male mice, 3.2 x 10-6 for SLT in female mice. 8.0 x 10-6 for SLT in male Drosophila melanogaster (D.m.), 1.6 x 10~6 for SLRL test in male D.m. and 2.0 x 10-6 for SLRL test in female D.m. They were found to be very similar across the species with some variation between loci and tests. Using much of the same data and estimates of male generation time of 30 years for humans, 9 months for mouse, and 18 days for D.m. , male mutation rates per year are calculated. Varying over 3 orders of magnitude, the results do not support a time-dependent mechanism for mutation rates. These germlines are described in detail and estimations of numbers of germcell divisions per generation are made. They are 399 for a 30-year-old human male, 31 for a human female, 62 for a 9-monin-oid mouse maie, 25 for a mouse female, 35-5 for an 18-day-old D.m. male and 36.5 for a 25-day-old D.m. female. Comparisons of mutation rates per cell division, along with data regarding parental origin of human mutations and male-driven molecular evolution, provide support for an effect of cell division upon mutation rates, but with additional factors which cause