Miracle-dz/UniFAR-FTUs huggingface dataset:
Rhomboid family intramembrane proteases in prokaryotes: mechanism, substrate repertoires and biological functions in the Gram-positive bacterium Bacillus subtilis.

Rhomboid proteases are a class of serine intramembrane proteases, a large family of enzymes that catalyze the proteolytic cleavage of membrane proteins within their transmembrane regions, in the hydrophobic environment of cellular lipid membranes. Rhomboid proteases were discovered in 2001 in Drosophila. In their pioneering study, Lee et al. identified the essential role of Rhomboid-1 protein (Rhom-1), which proteolytically activates the epidermal growth factor (EGF) receptor signaling pathway, in the early stages of fly eye development. Members of the rhomboid superfamily – active proteases (rhomboids) as well as their catalytically-dead counterparts (rhomboid-like proteins, including iRhoms and Derlins) are widely conserved, implying their biological significance. Rhomboids are present in all kingdoms of life from archea to humans, while proteolytically inactive rhomboid-like proteins are present in eukaryotes only. Rhomboid superfamily proteins play roles in a wide range of processes, as diverse as signaling in metazoan development, mitochondrial biogenesis in yeast, host-cell invasion by protozoan parasites, protein quality control in the endoplasmic reticulum (ER) or bacterial quorum sensing. Rhomboids are the best understood intramembrane proteases from a structural and mechanistic points of view. Most of the work has been done on the rhomboid protease GlpG from the Gram-negative bacterium Escherichia coli. The thesis focuses on the mechanistic characterization of the intramembrane rhomboid protease GlpG from the Gram-negative bacterium Escherichia coli and on the identification of biological role of YqgP from the Gram-positive bacterium Bacillus subtilis. Based on genetic analyses, GlpG-like and YqgP-like proteases are highly populated among bacterial rhomboid proteins and are also present in several pathogens such as Gram-negative Salmonella or Shigella and Gram-positive Listeria or Staphylococcus. To refine our knowledge of the mechanistic principles covering substrate specificity, we have mapped the amino acid preferences of the GlpG rhomboid protease and developed its substratederived inhibitors. The tools that we developed for GlpG rhomboid, were subsequently used to characterise the biological function of YqgP. We analysed the degradome and the interactome of YqgP in Bacillus subtilis in vivo and identified MgtE, the main magnesium transporter in Bacillus subtilis, as the natural substrate of YqgP. Finally, we showed that YqgP cooperates with the membrane embedded AAA+ protease FtsH during membrane protein quality control in Bacillus subtilis, representing an ancestral membrane protein degradation system conceptually similar to the eukaryotic ER associated degradation.
GeneWeld: a method for efficient targeted integration directed by short homology

Choices for genome engineering and integration involve high efficiency with little or no target specificity or high specificity with low activity. Here, we describe a targeted integration strategy, called GeneWeld, and a vector series for gene tagging, pGTag (plasmids for Gene Tagging), which promote highly efficient and precise targeted integration in zebrafish embryos, pig fibroblasts, and human cells utilizing the CRISPR/Cas9 system. Our work demonstrates that in vivo targeting of a genomic locus of interest with CRISPR/Cas9 and a donor vector containing as little as 24 to 48 base pairs of homology directs precise and efficient knock-in when the homology arms are exposed with a double strand break in vivo. Given our results targeting multiple loci in different species, we expect the accompanying protocols, vectors, and web interface for homology arm design to help streamline gene targeting and applications in CRISPR compatible systems. In Brief Wierson et al. describe a targeted integration strategy, called GeneWeld, and a vector series for gene tagging, pGTag, which promote highly efficient and precise targeted integration in zebrafish, pig fibroblasts, and human cells. This approach establishes an effective genome engineering solution that is suitable for creating knock-in mutations for functional genomics and gene therapy applications. The authors describe high rates of germline transmission (50%) for targeted knock-ins at eight different zebrafish loci and efficient integration at safe harbor loci in porcine and human cells.
Algoritmo para Codificação e Decodificação de Códigos Polares visando Aplicações em Sistemas 5G

A implementacao das comunicacoes sem fio de quinta geracao (5G) estao previstas para 2020 e tornaram-se um grande alvo para diversas pesquisas. Deste modo um dos estudos desenvolvidos sao sobre tecnicas de codificacao de canal, pois ao utilizar uma boa tecnica de codificacao e possivel se aproximar cada vez mais da capacidade do canal, que e a melhor forma de transmitir uma informacao de forma confiavel atraves de um canal de comunicacao. A codificacao polar e um dos candidatos para compor os sistemas 5G em comparacao com outras codificacoes conhecidas, devido ao bom desempenho e baixa complexidade de implementacao, [2]. Codigos polares sao codigos de bloco (N, K), contruidos a partir da combinacao e divisao de canais de comprimento finito, utilizando a tecnica proposta por [1] chamada de polarizacao de canal. Neste trabalho, apresentamos uma implementacao computacional da codificacao e decodificacao de codigos polares com taxa R = 1/2 e diferentes comprimentos N , utilizando o software MATLAB. [. ]
The BK channel gating ring is strongly coupled to the voltage sensor

The open probability of large conductance voltage- and calcium-dependent potassium (BK) channels is regulated allosterically by changes in the transmembrane voltage and intracellular concentration of divalent ions (Ca2+ and Mg2+). The divalent cation sensors reside within the gating ring formed by eight Regulator of Conductance of Potassium (RCK) domains, two from each of the four channel subunits. Overall, the gating ring contains 12 sites that can bind Ca2+ with different affinities. Using patch-clamp fluorometry, we have shown robust changes in FRET signals within the gating ring in response to divalent ions and voltage, which do not directly track open probability. Only the conformational changes triggered through the RCK1 binding site are voltage-dependent in presence of Ca2+. Because the gating ring is outside the electric field, it must gain voltage sensitivity from coupling to the voltage-dependent channel opening, the voltage sensor or both. Here we demonstrate that alterations of voltage sensor dynamics known to shift gating currents produce a cognate shift in the gating ring voltage dependence, whereas changing BK channels’ relative probability of opening had little effect. These results strongly suggest that the conformational changes of the RCK1 domain of the gating ring are tightly coupled to the voltage sensor function, and this interaction is central to the allosteric modulation of BK channels.
Experimentos de ciencias naturales para grado sexto a partir de cultivo de café

El aprendizaje de las ciencias naturales en escuelas rurales ha ido perdiendo relevancia para los estudiantes debido a la implementacion de modelos como Escuela Nueva, el cual se basa en una ensenanza multigrado, que limita al docente en cuanto al desarrollo de actividades experimentales que permitan reforzar los conceptos abordados en el plan de estudios. Por la razon anterior, el principal objetivo de la investigacion fue el diseno e implementacion de una secuencia experimental para grado sexto fundamentada en el modelo de cognicion situada, el programa escuela y cafe, los proyectos pedagogicos productivos y la cultura caficultora del municipio de Quipile (Cundinamarca). La metodologia utilizada fue Investigacion-Accion cuya etapa inicial dio origen a cuatro talleres experimentales: estudio de suelos, germinacion de semilla de cafe, elaboracion de filtro de agua y fabricacion de productos alimenticios a base de cafe. Como resultado de la investigacion fue posible encontrar que los estudiantes conocen las etapas del cultivo de cafe, pero utilizan terminos cotidianos transmitidos por sus padres para explicar los diferentes procesos que suceden en el. Con la aplicacion de los diferentes talleres experimentales fue posible que los estudiantes estructuran su lenguaje a uno mas cientifico al explicar caracteristicas del cultivo, ya que utilizaron conceptos como tejido embrionario, transporte activo y pasivo, mezcla homogenea entre otros. Ademas fue evidente que al relacionar un fenomeno cultural y economico del contexto con los contenidos de la escuela, se propicia una mayor comprension de los conceptos cientificos y se les atribuye un mayor significado para la realidad del estudiante.
Influence of maternal factors on the rumen microbiome and subsequent host performance1

© The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com Transl. Anim. Sci. 2018.2:S101–S105 doi: 10.1093/tas/txy058
Nutritive compositions of locally available jackfruit seeds (Artocarpus heterophyllus) in Bangladesh

Abstract The nutritive value of jackfruit seeds was investigated using the following parameters -proximate composition and mineral composition. The aim of this study is to determine the physico-chemical properties of jackfruit seeds. Three types of jackfruits namely Khaja (green, hard and smooth, with juicy pulp and small seeds), Gala (rough, soft, with thin pulp, not very juicy, and large seeds) and Durosha (rough, soft, intermediate size of pulps and seeds) were taken under investigation. The fruits were cut, the seeds removed, sliced, dried at 60 °C for 24 hrs, packaged in polyethelyne bag and kept in a refrigerator (~20±2° C). The moisture content, dry matter and ash content was found around 21.10- 42.25%, 57.75- 78.90% and 2.13- 4.07% respectively among the varieties. A good amount of protein content was found in all varieties of jackfruit seeds, it ranged from 13-18%. Crude fibre content of seed varied from 1.56-2.60%. Jackfruit is a good source of many mineral contents like N, P, K, Ca, Mg, S, Zn, Cu etc. Starch content in seed was found from 12.86- 17.90%. The information achieved from the study could be helpful for the students, teachers, scientists who has been engaged with the biochemical analysis of different types of jackfruits in Bangladesh.
Gene co-expression modulating terpene metabolism is associated with plant anti-herbivore defence during initial flowering stages

To protect themselves from herbivore attacks, especially during early developmental stages, plants produce volatiles possessing various ecological and physiological functions. Here, we identified three genes cloned from Lavandula angustifolia. These genes, designated as LaTPS7, LaTPS8, and LaCYP71D582, were hypothesized to be active in plant defense during early developmental stages. The in-vitro assays showed LaTPS7 produced eight compounds including camphene, myrcene, limonene etc. and LaTPS8 catalyzed nine volatiles from α-pinene, sylvestrene to fenchol etc. using geranyl diphosphate (GPP) and nerolidyl diphosphate (NPP) as substrate separately. However, LaTPS7 present in plastids only, synthesized limonene within Nicotiana benthamiana. Limonene was then converted into carveol by LaCYP71D582 present in the endoplasmic reticulum. LaTPS8, also located in plastids, synthesized α-pinene and sylvestrene. Odour response of aphids (Myzus persicae) and ladybugs (Harmonia axyridis) showed that volatiles from transgenic tobacco leaves repelled aphids and attracted ladybugs. LaTPS7 promoter GUS stain assay in Arabidopsis thaliana showed that LaTPS7 displayed a wound-induced expression in leaves. Together, our findings show that these compounds and gene-expression pattern played important roles in protecting plants during vulnerable stages. More practically, these plant tactics can be exploited in agriculture to decrease the use of insecticides, thereby contributing to improved human and environmental health.
Modelação espacial aplicada ao Ordenamento do Território Inteligente

a análise, modelação e simulação, com recurso a informação geográfica com resolução muito alta, da transformação de uso e ocupação do solo à escala local (município de Portimão, Portugal). As regras de transição foram definidas a partir de um modelo espacial de regressão logística, tendo sido posteriormente incorporadas num modelo convencional de AC. A dimensão e as tendências gerais da transformação de uso e ocupação do solo foram analisadas e estimadas através da aplicação das cadeias de Markov. Os principais resultados da investigação indicam que as áreas artificializadas no município de Portimão têm vindo a crescer massivamente, em particular junto dos principais aglomerados urbanos e ao longo da costa, revelando uma dispersão urbana precoce e intensiva ao longo do tempo. Celulares; uso ocupação do solo
Strengths Work in Social Work Education at HAWK, Germany

Professions in human services are committed to supporting the personal development of the people they work with. According to the international definition of social work1, empowerment and enhancing the wellbeing of people is a central mission of social workers. However, in times of budget cuts, austerity programs and an increasing caseload this mission proves to be an enormous challenge for social workers or other practitioners as well for clients. For the last 30 years, neoliberal management strategies have been implemented in many countries with the goal to make care systems more efficient. The management strategies put a focus on output rather than outcome. With these changes in place, the clients’ interests often cannot be focused on intensely anymore. Instead of empowering people, professionals in human services are held back and constrained.
Vehicle detection using histogram of oriented gradients and real adaboost

The vehicle detection system is an important technology because it has many applications in traffic fields such as traffic monitoring, counting the number of vehicles passing, calculating the speed of an oncoming vehicle, and so on. Histogram Of Oriented Gradients (HOG) is a feature descriptor used for object detection. HOG describes features based on some local histogram of gradient orientation weighted by gradient magnitude. Real AdaBoost is a learning algorithm which combined weak classifier into a strong classifier that represents the final output of the boosted classifier. This research aims to detect vehicles in the static image using a Histogram Of Oriented Gradients methods and Real Adaboost. The steps of vehicle detection are pra-processing, feature extraction process with HOG, and classification process with Real AdaBoost. The testing result shows that the system can detect vehicles with an accuracy level of 91.78 % from 292 testing images, from 257 images of the vehicle and 35 images of not vehicle.
Analysis of Artificial Neural Network Point Forecasting Models and Prediction Intervals for Solar Irradiance Estimation

An accurate knowledge on solar irradiance prediction is particularly required for proper development and planning of Photovoltaic (PV) energy systems. The main purpose of the present research is to assess the accuracy of Artificial Neural Networks (ANN) short-term forecast of univariate solar irradiance time series, with conventional point prediction and Prediction Intervals (PIs), comparing models. The Lower Upper Bound Estimation trained with Particle Swarm Optimization (PSO-LUBE) was used for PIs estimation. Solar irradiance data collected from a station in Amazon region in Brazil was used to train and test the models. Results demonstrate that all ANN models yield good accuracy in terms of prediction error: 8.1-8.5% for normalized root Mean Square Error (nRMSE), 5.8-6.0% for normalized Mean Absolute Error (nMAE) and 94-95% for determination coefficient (R2). However, due to the accuracy of PI information (Coverage Probability = 94.94% and PI Normalized Average Width = 32.50%), PSO-LUBE was the best method tested for decision-making.
Laktosa, Lemak Dan Produksi Susu Pada Sapi Perah Laktasi yang Diberi Total Mixed Ration Berbasis Jerami Jagung Teramoniasi

Tujuan dari limbah penelitian ini mengevaluasi efek dari metode pemberian makan pada total ransum campuran (TMR) pada jagung strawbase amoniak pada susu laktosa, lemak susu, dan produksi susu pada sapi perah. Percobaan dilakukan dengan menggunakan rancangan acak kelompok (RAK) dengan 3 perlakuan dan 5 kelompok berdasarkan bulan laktasi. Perlakuan diaplikasikan pada T0 = non TMR, T1 = TMR pada dasar jerami jagung, dan T2 = TMR pada dasar jerami jagung teramoniasi. Data dianalisis dengan analisis prosedur varians dan diikuti oleh uji kontras ortogonal. Hasil uji kontras orthogonal menunjukkan bahwa rata-rata susu laktosa dan lemak susu pada perlakuan non TMR (T0) tidak berbeda nyata (p> 0,05) dibandingkan dengan TMR pada basis jerami jagung dengan dan tanpa amoniak. Rata-rata susu laktosa dan lemak susu pada perlakuan TMR pada basis amoniak jerami jagung (T2) secara signifikan lebih tinggi dibandingkan dengan TMR pada basis jerami jagung tanpa amoniak (T1). Rata-rata produksi susu tidak dipengaruhi oleh pengobatan (p> 0,05). TMR pada basis amoniak jerami jagung (T2) menghasilkan produksi tertinggi susu terkoreksi 4% lemak adalah 2085,83 kg / laktasi. Fenomena ini menunjukkan bahwa TMR pada amonium jagung berbasis ampas efektif diterapkan oleh masyarakat untuk meningkatkan produktivitas sapi perah.
A phylogenetic study of Pouteria (Sapotaceae) in Malesia and Australasia using morphology and molecular data

The genus Pouteria Aublet is a pan-tropical group and many of its species produce high quality timber and edible fruit. In 1991, based on morphological characters Pennington combined the genus Planchonella Pierre into Pouteria, expanding the latter genus to nine sections and 325 species. However, many Planchonella species were not included in his account and doubt remains about the generic limits of Pouteria sensu Pennington. This study has examined relationships amongst genera in the tribe Chrysophylleae and infrageneric relationships of the species of genus Pouteria in Malesia and Australasia within a phylogenetic framework. The hypotheses of monophyly of genus Pouteria and tribe Chrysophylleae have been tested using DNA sequences drawn from two genes (nuclear ITS and chloroplast ndhV), and 64 morphological characters scored from vegetative and reproductive organs. The analysis included 47 representative species in genus Pouteria in Malesia and Australasia that were previously classified under Planchonella and Pouteria sensu lato and currently classified as Pouteria sensu Pennington (Pennington 1991). Other genera from Sapotaceae included in the analysis were Chrysophyllum, Niemeyera, Pichonia, Pycnandra and Xantolis (tribe Chrysophyllae) and Mimusops, Palaquium, Manilkara (from other tribes) as outgroups. The previously published ndhF and ITS sequences of twelve New Caledonian Pouteria and seventeen species of other genera in Chrysophylleae: Pycnandra, Niemeyera, Leptostylis, Pichonia, and Xantolis from GenBank (Bartish et al. 2005) are also
Meaningful employment contributes to an improved quality of life

This paper attempts to explore the role of meaningful employment for enhancing the quality of life. Globally all the academicians, politicians, developmental economists and social scientists are concerned with enhancing the possibilities and capabilities of human beings so that they can spend their life in a qualitative and desirable way. Quality of life is complex and broader term which has many dimensions such as long and healthy life, education and decent standard of living. The stability of the job, pay according to the work, recognition of the ability, fair treatment by employer and all such conditions which contribute to economic prosperity of an individual and this in turn leads to high quality of life. The paper seeks to highlight the role of meaningful employment in enhancing the quality of life through job satisfaction, fulfillment of basic needs and satisfaction of social and psychological needs of an individual. Quality of individual lives is critical for the overall development of any nation especially in this globalized world.
"Beyond black and blue" : intimate partner violence as a form of family violence against women and common mental disorders in Mumbai informal settlements

The thesis investigates family violence against women living in informal settlements in Mumbai, and its associations with mental health. Globally and in India, a third of women experience violence from an intimate partner in their lifetime, an estimate that would be higher if it accounted for other perpetrators within the family. The thesis includes qualitative and quantitative studies. The quantitative study was a survey of 482 women that investigated violence in detail, including its types, perpetrators, and timescales. Symptoms of common mental disorders were assessed with the General Health Questionnaire-12 and levels of self-esteem with the Rosenberg self-esteem questionnaire. Associations were explored through multivariable linear and ordinal logistic regression, adjusted for socio-demographic covariates. The qualitative study included semi-structured individual interviews and focus group discussions with 33 women, exploring narratives of violence, responses, coping, and resilience. Analysis took a Framework approach. The prevalence of violence was high - 44% over a lifetime - but in line with other national surveys. More women reported emotional violence than other forms. In-laws were the main perpetrators of emotional and economic violence, and husbands of physical and sexual violence. Emotional violence showed the strongest positive association with symptoms of common mental disorders and lower self-esteem. In the qualitative study, women described patterns of violence that often included the marital family as perpetrators. Responses to violence included feelings, thoughts, and behaviours ranging between active and passive. Women described a ceiling of tolerance that influenced their responses to violence and was informed by their context and previous experiences. The thesis adds to the current literature by exploring violence in detail and combining survey data with women’s narratives. It highlights the need to further investigate family violence and emotional violence, particularly in other South Asian settings where patterns of patrilocal residence and joint families are common.