Miracle-dz/UniFAR-FTUs huggingface dataset:
Intestine-specific expression of Apobec-1 rescues apolipoprotein B RNA editing and alters chylomicron production in Apobec1−/− mice[S]

Intestinal apolipoprotein B (apoB) mRNA undergoes C-to-U editing, mediated by the catalytic deaminase apobec-1, which results in translation of apoB48. Apobec1−/− mice produce only apoB100 and secrete larger chylomicron particles than those observed in wild-type (WT) mice. Here we show that transgenic rescue of intestinal apobec-1 expression (Apobec1Int/O) restores C-to-U RNA editing of apoB mRNA in vivo, including the canonical site at position 6666 and also at approximately 20 other newly identified downstream sites present in WT mice. The small intestine of Apobec1Int/O mice produces only apoB48, and the liver produces only apoB100. Serum chylomicron particles were smaller in Apobec1Int/O mice compared with those from Apobec1−/− mice, and the predominant fraction of serum apoB48 in Apobec1Int/O mice migrated in lipoproteins smaller than chylomicrons, even when these mice were fed a high-fat diet. Because apoB48 arises exclusively from the intestine in Apobec1Int/O mice and intestinal apoB48 synthesis and secretion rates were comparable to WT mice, we were able to infer the major sites of origin of serum apoB48 in WT mice. Our findings imply that less than 25% of serum apoB48 in WT mice arises from the intestine, with the majority originating from the liver.
GSTM1 polymorphism in patients with primary open-angle glaucoma.

Primary open-angle glaucoma (POAG) is characterized by loss of retinal ganglion cells, optic nerve damage and irreversible loss of visual field. Glaucoma is the second leading cause of blindness worldwide. It was estimated that in 2010 there were about 60.5 million glaucoma cases worldwide; among these patients, 4.5 million will become bilaterally blind. Glutathione S-transferases (GST) are a group of drug-metabolizing enzymes of phase-II that act in the detoxification of xenobiotics and inactivate end-products formed as secondary metabolites during oxidative stress. Through PCR amplification, we analyzed the GSTM1 gene in DNA samples from 25 patients with POAG and 25 controls; 14 of the patients presented the GSTM1 gene null polymorphism while only eight of the control group had this gene.Although the POAG patients had a higher frequency of GSTM1, the difference was not significant (P = 0.0874); this lack of significance could be due to the small sample size.
Circadian Time Organization of Professional Firemen: Desynchronization—Tau Differing from 24.0 Hours—Documented by Longitudinal Self-assessment of 16 Variables

*This work is dedicated to the memories of Drs. Israel Ashkenazi and Erhard Haus, outstanding pioneers of medical chronobiology, dear colleagues, and distinguished gentlemen of exceptional human qualities.We investigated the circadian synchronization/desynchronization (by field-study assessment of differences in period, τ, of 16 coexisting and well-documented rhythms) of 30 healthy firemen (FM) exposed to irregular, difficult, and stressful nocturnal work hours who demonstrated excellent clinical tolerance (allochronism). Three groups of FM were studied (A = 12 FM on 24-h duty at the fire station; B = 9 FM on 24-h duty at the emergency call center; C = 9 day-shift administrative FM) of mostly comparable average age, body mass index, career duration, chronotype—morningness/eveningness, and trait of field dependence/independence. The self-assessed 16 circadian rhythms were (i) physiological ones of sleep-wake (sleep log), activity-rest (actography), body temperature (internal transmitter pill probe), right- and left-hand grip strength (hand dynamometer), systolic and diastolic blood pressure (BP) plus heart rate (ambulatory BP monitoring device); (ii) psychological ones (visual analog self-rating scales) of sleepiness, fatigue, fitness for work, and capacity to cope with aggressive social behavior; and (iii) cognitive ones of eye-hand skill and letter cancellation, entailing performance speed (tasks completed/unit time) and accuracy (errors). Data (4–6 time points/24 h; 2 591 480 values in total) were gathered continuously throughout two 8-d spans, one in winter 2010–2011 and one in summer 2011. Each of the resulting 938 unequal-interval time series was analyzed by a special power spectrum analysis to objectively determine the prominent τ. The desynchronization ratio (DR: number of study variables with τ = 24.0 h/number of study variables × 100) served to ascertain the strength/weakness of each rhythm per individual, group, and season. The field study confirmed, independent of group and season, coexistence of rather strong and weak circadian oscillators. Interindividual differences in DR were detected between groups and seasons (χ2, correlation tests, analysis of variance [ANOVA]). Moreover, in each group, both in winter and summer, a normal distribution was observed in the number of FM with rhythms with τ = 24.0 h, e.g., ranging from 5/16 (large desynchronization) to 16/16 (no desynchronization). Such a normal distribution with intraindividual stability over time (i.e., seasons) is consistent with the hypothesis of an inherited origin of a differential propensity to circadian desynchronization and which is supported by the distribution of τs in winter and summer following the Dian-Circadian Genetic Model, i.e., with τ = 24.0 h, τ = 24.0 h + n(0.8 h), and τ = 24.0 h − n(0.8 h).
The Amplitude of Circadian Oscillations: Temperature Dependence, Latitudinal Clines, and the Photoperiodic Time Measurement

This paper develops several propositions concerning the lability of the amplitude of Drosophila circadian pacemakers. The first is that the amplitude of the pacemaker's motion, unlike its period, is markedly temperature-dependent. The second is that latitudinal variation in pacemaker amplitude (higher in the north) is responsible for two very different sets of observations on Drosophila circadian systems at successively higher latitudes. One of these is a cline in D. auraria's phase-shifting response to light, which steadily weakens in a succession of more northerly strains. The other, concerning D. littoralis in the very far north, is a cline in the rate at which eclosion activity becomes arrhythmic (the circadian rhythm damps out) in constant darkness; damping is faster in the north. The third proposition concerns a plausible selection pressure for the cline in pacemaker amplitude that we propose underlies the two directly observed clines. Two points are emphasized: (1) The amplitude of the pacemaker's daily oscillation declines as the duration of the entraining light pulse (photoperiod) is increased; and (2) the duration of the daily photoperiods throughout the breeding season is steadily increased as one moves toward the poles. Selection for conservation of pacemaker amplitude (during the breeding season) would produce the latitudinal cline we propose. The fourth, and final proposition is that since the amplitude of the pacemaker's daily motion responds systematically to change in photoperiod, amplitude is clearly one way—and a temperature-dependent way—in which insect circadian systems may sense seasonal change. These propositions concerning the temperature and latitude dependence of pacemaker amplitude may be relevant to a wider array of circadian pacemakers than Drosophila.
Four-wave-mixing suppression effect of dispersion varying fiber

This letter describes the four-wave-mixing (FWM) suppression effect of dispersion varying fiber (DVF) whose chromatic dispersion increases (or decreases) along its length. The FWM suppression performance is investigated for the dispersion variation rate and its cycle number in the DVF. Moreover, it is clarified experimentally that the DVF functions efficiently in wavelength division multiplexing (WDM) systems with an input power of more than 10 dBm/ch.
Enhanced viability imaging: Improved contrast in myocardial delayed enhancement using dual inversion time subtraction

In delayed contrast‐enhanced MRI for the assessment of myocardial viability, the TI time in a gated inversion‐recovery segmented gradient echo sequence is usually selected to null signal from normal myocardium. Although this TI time generates good contrast between the enhancing infarcted tissue and normal myocardium, there is usually less contrast between the infarct and the blood pool. A subtractive technique utilizing two acquisitions at a long and short TI time is proposed to improve the delineation between infarct‐blood and infarct‐myocardium. The concept was demonstrated in six mongrel dogs with reperfused myocardial infarction. Infarct‐normal myocardium contrast (signal difference) using the proposed enhanced viability imaging (ENVI) technique was 142 ± 50% (P < 0.001) that of standard magnitude inversion recovery (IR), while at the same TI time for the primary image, infarct‐blood contrast, was 247 ± 136% (P < 0.002) that of magnitude IR. Accounting for increased noise due to the subtraction, signal difference‐to‐noise ratios (SDNR) did not show a significant change for infarct‐myocardium but infarct‐blood SDNR for ENVI was 174 ± 105% that of magnitude‐IR (P < 0.03). Thus, marked improvement in the delineation of the infarcted zone was noted over a range of TI times. Magn Reson Med 53:1484–1489, 2005. © 2005 Wiley‐Liss, Inc.
Pathogenesis, Molecular Genetics, and Genomics of Mycobacterium avium subsp. paratuberculosis, the Etiologic Agent of Johne’s Disease

Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of Johne’s disease in ruminants causing chronic diarrhea, malnutrition, and muscular wasting. Neonates and young animals are infected primarily by the fecal–oral route. MAP attaches to, translocates via the intestinal mucosa, and is phagocytosed by macrophages. The ensuing host cellular immune response leads to granulomatous enteritis characterized by a thick and corrugated intestinal wall. We review various tissue culture systems, ileal loops, and mice, goats, and cattle used to study MAP pathogenesis. MAP can be detected in clinical samples by microscopy, culturing, PCR, and an enzyme-linked immunosorbent assay. There are commercial vaccines that reduce clinical disease and shedding, unfortunately, their efficacies are limited and may not engender long-term protective immunity. Moreover, the potential linkage with Crohn’s disease and other human diseases makes MAP a concern as a zoonotic pathogen. Potential therapies with anti-mycobacterial agents are also discussed. The completion of the MAP K-10 genome sequence has greatly improved our understanding of MAP pathogenesis. The analysis of this sequence has identified a wide range of gene functions involved in virulence, lipid metabolism, transcriptional regulation, and main metabolic pathways. We also review the transposons utilized to generate random transposon mutant libraries and the recent advances in the post-genomic era. This includes the generation and characterization of allelic exchange mutants, transcriptomic analysis, transposon mutant banks analysis, new efforts to generate comprehensive mutant libraries, and the application of transposon site hybridization mutagenesis and transposon sequencing for global analysis of the MAP genome. Further analysis of candidate vaccine strains development is also provided with critical discussions on their benefits and shortcomings, and strategies to develop a highly efficacious live-attenuated vaccine capable of differentiating infected from vaccinated animals.
Distinct set of kinases induced after retrieval of spatial memory discriminate memory modulation processes in the mouse hippocampus

Protein phosphorylation and dephosphorylation events play a key role in memory formation and various protein kinases and phosphatases have been firmly associated with memory performance. Here, we determined expression changes of protein kinases and phosphatases following retrieval of spatial memory in CD1 mice in a Morris Water Maze task, using antibody microarrays and confirmatory Western blot. Comparing changes following single and consecutive retrieval, we identified stably and differentially expressed kinases, some of which have never been implicated before in memory functions. On the basis of these findings we define a small signaling network associated with spatial memory retrieval. Moreover, we describe differential regulation and correlation of expression levels with behavioral performance of polo‐like kinase 1. Together with its recently observed genetic association to autism‐spectrum disorders our data suggest a role of this kinase in balancing preservation and flexibility of learned behavior. © 2013 Wiley Periodicals, Inc.
Delay-Dependent Stability of Single-Loop Controlled Grid-Connected Inverters with LCL Filters

LCL filters have been widely used for grid-connected inverters. However, the problem that how time delay affects the stability of digitally controlled grid-connected inverters with LCL filters has not been fully studied. In this paper, a systematic study is carried out on the relationship between the time delay and stability of single-loop controlled grid-connected inverters that employ inverter current feedback (ICF) or grid current feedback (GCF). The ranges of time delay for system stability are analyzed and deduced in the continuous s-domain and discrete z-domain. It is shown that in the optimal range, the existence of time delay weakens the stability of the ICF loop, whereas a proper time delay is required for the GCF loop. The present work explains, for the first time, why different conclusions on the stability of ICF loop and GCF loop have been drawn in previous studies. To improve system stability, a linear predictor-based time delay reduction method is proposed for ICF, while a time delay addition method is used for GCF. A controller design method is then presented that guarantees adequate stability margins. The delay-dependent stability study is verified by simulation and experiment.
The reproductive cycle of the male sleep snake Sibynomorphus mikanii (Schlegel, 1837) from southeastern brazil

This study describes the male reproductive cycle of Sibynomorphus mikanii from southeastern Brazil considering macroscopic and microscopic variables. Spermatogenesis occurs during spring–summer (September–December) and spermiogenesis or maturation occurs in summer (December–February). The length and width of the kidney, the tubular diameter, and the epithelium height of the sexual segment of the kidney (SSK) are larger in summer–autumn (December–May). Histochemical reaction of the SSK [periodic acid‐Schiff (PAS) and bromophenol blue (BB)] shows stronger results during summer–autumn, indicating an increase in the secretory activity of the granules. Testicular regression is observed in autumn and early winter (March–June) when a peak in the width of the ductus deferens occurs. The distal ductus deferens as well as the ampulla ductus deferentis exhibit secretory activities with positive reaction for PAS and BB. These results suggest that this secretion may nourish the spermatozoa while they are being stored in the ductus deferens. The increase in the Leydig cell nuclear diameter in association with SSK hypertrophy and the presence of sperm in the female indicate that the mating season occurs in autumn when testes begin to decrease their activity. The peak activity of Leydig cells and SSK exhibits an associated pattern with the mating season. However, spermatogenesis is dissociated of the copulation characterizing a complex reproductive cycle. At the individual level, S. mikanii males present a continuous cyclical reproductive pattern in the testes and kidneys (SSK), whereas at the populational level the reproductive pattern may be classified as seasonal semisynchronous. © J. Morphol. , 2013. © 2012 Wiley Periodicals, Inc.
Secondary prevention clinics for coronary heart disease: four year follow up of a randomised controlled trial in primary care

Abstract Objectives: To evaluate the effects of nurse led clinics in primary care on secondary prevention, total mortality, and coronary event rates after four years. Design: Follow up of a randomised controlled trial by postal questionnaires and review of case notes and national datasets. Setting: Stratified, random sample of 19 general practices in north east Scotland. Participants: 1343 patients (673 intervention and 670 control) under 80 years with a working diagnosis of coronary heart disease but without terminal illness or dementia and not housebound. Intervention: Nurse led secondary prevention clinics promoted medical and lifestyle components of secondary prevention and offered regular follow up for one year. Main outcome measures: Components of secondary prevention (aspirin, blood pressure management, lipid management, healthy diet, exercise, non-smoking), total mortality, and coronary events (non-fatal myocardial infarctions and coronary deaths). Results: Mean follow up was at 4.7 years. Significant improvements were shown in the intervention group in all components of secondary prevention except smoking at one year, and these were sustained after four years except for exercise. The control group, most of whom attended clinics after the initial year, caught up before final follow up, and differences between groups were no longer significant. At 4.7 years, 100 patients in the intervention group and 128 in the control group had died: cumulative death rates were 14.5% and 18.9%, respectively (P=0.038). 100 coronary events occurred in the intervention group and 125 in the control group: cumulative event rates were 14.2% and 18.2%, respectively (P=0.052). Adjusting for age, sex, general practice, and baseline secondary prevention, proportional hazard ratios were 0.75 for all deaths (95% confidence intervals 0.58 to 0.98; P=0.036) and 0.76 for coronary events (0.58 to 1.00; P=0.049) Conclusions: Nurse led secondary prevention improved medical and lifestyle components of secondary prevention and this seemed to lead to significantly fewer total deaths and probably fewer coronary events. Secondary prevention clinics should be started sooner rather than later. What is already known on this topic Several effective interventions exist for the secondary prevention of coronary heart disease, but implementing them in practice has proved difficult Secondary prevention programmes for coronary heart disease have improved short term outcomes such as processes of care and quality of life What this study adds Short term improvements in uptake of secondary prevention produced by nurse led clinics are maintained in the longer term Improved medical and lifestyle components of secondary prevention produced by nurse led clinics seem to lead to fewer total deaths and coronary events
Crevasse detection with GPR across the Ross Ice Shelf, Antarctica

We have used 400-MHz ground penetrating radar (GPR) to detect crevasses within a shear zone on the Ross Ice Shcelf, Antarctica, to support traverse operations. The transducer was attached to a 6.5-m boom and pushed ahead of an enclosed tracked vehicle. Profile speeds of 4.8-11.3 km / hr allowed real- time crevasse image display and a quick, safe stop when required. Thirty-two crevasses were located with radar along the 4.8 km crossing. Generally, crevasse radar images were characterized by dipping reflections above the voids, high-amplitude reflections originating from ice layers at the base of the snow-bridges, and slanting, diffracting reflections from near-vertical crevasse walls. New cracks and narrow crevasses (40 cm width) show no distinct snow bridge structure, few diffractions, and a distinct band where pulse reflections are absent. Wide (0.5-5.0 m), vertical wall crevasses show distinct dipping snow bridge layering and intense diffractions from ice layers near the base of the snow bridge. Pulse reflections are absent from voids beneath the snow bridges. Old, wide (3.W.O m) and complexly shaped crevasses show well-developed, broad, dipping snow-bridge layers and a high-amplitude, complex, diffraction pattern. The crevasse mitigation process, which included hot-water drilling, destroying the bridges with dynamite, and back-filling with bulldozed snow, afforded an opportunity to ground-truth GPR interpretations by comparing void size and snow-bridge geametry with the radar images. Second season radar profiles colllected along the identical flagged route confirmed stability of the filled crevasses and identified eight new cracks opened by ice extension. Our experiment demonstrates the utility and reliability of real-time crevasse detection with GPR. Keywords-crevasse, snow stratigraphy, ground penetrating radar, traverse, McMurdo shear zone
The Changing Roles of Personnel Managers: Old Ambiguities, New Uncertainties

There have been notable attempts to capture the changing nature of personnel roles in response to major transformations in the workplace and the associated rise of 'HRM'. A decade ago Storey (1992) explored the emerging impact of workplace change on personnel practice in the UK and proposed a new fourfold typology of personnel roles: 'advisors', 'handmaidens', 'regulators' and 'changemakers'. Have these four roles changed now that HRM has increasingly become part of the rhetoric and reality of organizational performance? If Storey's work provides an empirical and analytical benchmark for examining issues of 'role change', then Ulrich's (1997) work in the USA offers a sweeping prescriptive end-point for the transformation of personnel roles that has already been widely endorsed by UK practitioners. He argues that HR professionals must overcome the traditional marginality of the personnel function by embracing a new set of roles as champions of competitiveness in delivering value. Is this a realistic ambition? The new survey findings and interview evidence from HR managers in major UK companies presented here suggests that the role of the personnel professional has altered in a number of significant respects, and has become more multifaceted and complex, but the negative counter-images of the past still remain. To partly capture the process of role change, Storey's original fourfold typology of personnel roles is re-examined and contrasted with Ulrich's prescriptive vision for the reinvention on the HR function. It is concluded that Storey's typology has lost much of its empirical and analytical veracity, while Ulrich's model ends in prescriptive overreach by submerging issues of role conflict within a new rhetoric of professional identity. Neither model can adequately accommodate the emergent tensions between competing role demands, ever-increasing managerial expectations of performance and new challenges to professional expertise, all of which are likely to intensify in the future. Copyright Blackwell Publishing Ltd 2003.
The social media logic of political interaction: Exploring citizens' and politicians' relationship on Facebook and Twitter

This paper examines citizens’ interactions with politicians in social media in what is referred to as an everyday context, unmediated by news media. Through a representative survey, we compare the influence of a series of socio-demographical variables such as political interest, age, gender and education on the interaction levels between citizens and politicians. The article argues that the social media logic can be operationalized into “connected affordances”: Redistribution, Interacting and Acknowledging — which are the three types of user practices on Facebook and Twitter. The study finds that Facebook is a service where “ordinary” people engage in political interaction with politicians — and receive replies from politicians — while Twitter is mostly used by just a small group of the population for these purposes. Hence, the popularity of Facebook could be seen as allowing for new connections between citizens and politicians without news media as mediators. Our results suggest that the stronger the political interest the citizens express, the more connective affordances are utilized — such as commenting and sharing. Political interest also impacts what response citizens receive from political actors in social media.