Miracle-dz/UniFAR-FTUs huggingface dataset:
HAGLROS is overexpressed and promotes non-small cell lung cancer migration and invasion.

INTRODUCTION Non-small cell lung cancer was one of the most common and deadly cancers worldwide. Long non-coding RNAs had been implicated in multiple human cancers, including non-small cell lung cancer. In this study, we focused on a novel long non-coding RNA, HAGLROS, in non-small cell lung cancer. MATERIAL AND METHODS In this study, we used GEPIA dataset to analyse the expression levels of HAGLROS in non-small cell lung cancer samples and normal tissues. Then, we analysed Kaplan-Meier Plotter database to reveal the association between HAGLROS expression and overall survival time in patients with non-small cell lung cancer. Moreover, we used small interfering RNA-mediated knockdown to reduce HAGLROS expression in A549 and H1299 cells. Cell Counting Kit-8 assay was used to detect the effect of HAGLROS on cell proliferation. Transwell assays were used to determine the effect of HAGLROS on cell migration and invasion. Co-expression analysis and bioinformatics analysis were conducted to predict the potential functions of HAGLROS in non-small cell lung cancer. RESULTS We identified HAGLROS was significantly overexpressed in non-small cell lung cancer samples compared to normal tissues. Higher expression of HAGLROS was significantly associated with shorter overall survival time in patients with non-small cell lung cancer. Moreover, we found knockdown of HAGLROS in non-small cell lung cancer cells remarkably suppressed tumour proliferation, migration and invasion. By conducting bioinformatics analysis, we found HAGLROS was involved in regulating multiple cancer-related pathways, including Spliceosome, DNA replication, cell cycle, chromosome segregation and sister chromatid segregation. CONCLUSIONS Our results for the first time demonstrated HAGLROS may serve as a target for new therapies in non-small cell lung cancer.
Adaptation and Validation of the Multi-Dimensional Perceived Autonomy Support Scale for Physical Education to the Spanish Physical Exercise Context

The interaction between the teacher and the student is essential in order to encourage adherence to physical exercise or sports by young people. In this sense, the support of the autonomy of the teacher for the students has been analyzed in a one-dimensional way. Therefore, the aim of this study was to adapt and validate the Multidimensional Scale of Support for Autonomy Perceived for Physical Education the context of Spanish to physical exercise, in order to have a multidimensional scale. A total of 2329 young people from various educational centers in Andalusia (Spain) participated in the study. The factorial structure of the questionnaire was examined through an exploratory factorial analysis and two confirmatory factorial analyses. In addition, an analysis of invariability by sex and age was carried out. The results reflected that the validated questionnaire showed adequate psychometric properties, being invariable with respect to sex and age.
MORPHOANATOMY OF NOTHOFAGUS ALESSANDRII SEEDS AND ITS USE IN THE VARIABILITY OF POPULATIONS

Nothofagus alessandrii es una especie endemica del area Mediterranea de Chile, de la cual no se cuenta con informacion sobre la estructura anatomica de sus semillas. Existen muy pocos estudios sobre la variabilidad morfologica y de germinacion entre sus poblaciones. El objetivo de este estudio fue describir la estructura morfoanatomica de semillas de N. alessandrii con el fin de comparar la morfologia y la germinacion entre cuatro poblaciones geograficamente separadas. Se seleccionaron semillas de cuatro origenes geograficos a lo largo de la distribucion latitudinal de la especie, y se midieron el tamano, la forma, el peso seco, y la germinacion. Los resultados mostraron que las semillas de N. alessandrii tienen una estructura anatomica similar a la de otras especies de la familia Fagaceae como Fagus sylvatica. No se encontraron diferencias significativas en las caracteristicas morfologicas de los lotes estudiados, ni tampoco en el poder germinativo, por lo que no se ha observado una variacion clinal entre poblaciones, aunque la poblacion localizada mas al sur presento variaciones en longitud y peso, sugiriendo la posibilidad de ecotipo diferenciado.
The use of the win odds in the design of non-inferiority clinical trials

ABSTRACT In 2012, Pocock and colleagues proposed the win ratio (WR) statistics for the analysis of controlled clinical trials with a composite endpoint. This approach takes into account clinical priorities among multiple components of the composite. Subjects in the test group are compared with subjects in the control group in all possible pairwise comparisons. The WR is calculated as the number of “winners” divided by the number of “losers” in the test group. The WR statistic is intuitive; however, it is not clear how it should be applied when the number of ties is substantial. To handle the ties, Dong et al. recently introduced the win odds (WO) statistics, which assigns 50% of the ties to both the numerator and the denominator of the WR statistic. In this work, we discuss the WO in the design of a Non-Inferiority (NI) trial with a composite endpoint, as ties in NI trials may reflect comparable treatment effect and the number of ties may be substantial. We extend the large sample inference for the WR to the WO and demonstrate the utility of the WO in the design of NI trials through simulations.
Raising Bioethical Consciousness in an Introductory Life Sciences Course.

VALUES QUESTIONS must be raised in science courses. In this article, I will outline my reasons for this belief, showing a shift in societal concerns and in the training of biology teachers and researchers. Objectives and exercises for lecture, laboratory, and discussion in high school and college survey courses will then be suggested. Social weighing of such issues as environmental development for recreation, industry, and wilderness; of energy versus ecology; of uses of genetic engineering, require an input not only of scientific facts but also of considered opinion. Traditionally the nonmajors biology course contained "interesting" units on pollution, birth control, and drugs. Biology majors memorized the carbon cycle, Krebs cycle, reproductive cycle, and DNA helix without being invited to explore their implications. Yet science is not value-free; that contention in itself implies a high value on objectivity. Visscher, in calling for a science based on a humanistic system of personal and group ethics (1975) noted that the intrinsic ethic of science is a devotion to verifiable truth. But biologists have learned values implicitly-values such as curiosity, search for truth, precision of knowledge-and since we have also valued hierarchy, people-centered values have ranked below objectivity. It was not part of the curriculum in our training to discuss scientists' responsibility for, or prediction of, possible applications of our discoveries. Did you discuss Social Darwinism, for example, in the genetics course? Appropriate use of herbicides in botany? Costs of synthetic vs. natural vitamins in biochemistry? Probably the personality profile of scientists as selfless, logical, choosing things over persons, avoiding the human world of complexity and ambiguity (Holton 1975), which drew us to science in the first place, was reinforced by our professional training, which stressed research design, regarded teaching as a nuisance, and deemphasized social issues. Now social circumstances have made us aware of our need to clarify our own values and to take part in social decisions. Molecular biologists have found it necessary to pause and consider appropriate containment of gene transplants (Berg et al. 1975). Experiments indicating subject willingness to inflict pain under experimenter authority have raised questions about appropriate research. Premedical students are urged to develop awareness of issues such as human dignity, autonomy, and nontherapeutic research prior to entry into the busy medical curriculum. Increased federal funding of research and education has increased the demand for social accountability and for the use of technology for national goals. Doubtless all will benefit from our asking: what is important to us? A conscious effort has been made to introduce such considerations into our life sciences courses at Worcester (Massachusetts) Polytechnic Institute (WPI). The WPI plan of education includes a degree requirement examining the reciprocal shaping of technology
A rapid method using Ricinus communis for the estimation of phloem translocation of xenobiotics

A simple and relatively rapid test has been developed that enables the phloem mobility of xenobiotics to be assessed. Compounds are applied by injection of an aqueous solution into the petioles of plants of castor bean (Ricinus communis var. Gibsonii), and phloem sap is collected from two incisions made in the stem below the treated leaves over the period of 2 to 4 h after application. Amounts of compounds in the sap are measured by liquid scintillation counting following thin-layer chromatography for radio-labelled samples or by high-pressure liquid chromatography for non-radio-labelled compounds. A semi-quantitative estimate of mobility in phloem can be obtained from these tests, including an estimate of the potential for long-distance transport via phloem. Mobilities in phloem measured for 28 compounds, mostly weak acids, are placed within a general framework for understanding the phloem transport of compounds in terms of their physico-chemical properties. This approach can be used to predict and interpret the behaviour of new compounds from a knowledge of their polarity and acid strength.
Flight activity, reproduction, and adult nutrition of the beet webworm, Spoladea recurvalis (Lepidoptera : Pyralidae)

The adult longevity, reproductive period, age-specific flight activity, suitable temperature range for flight, and effect of adult feeding on the subsequent flight of Spoladea recurvalis Fabricius were studied in the laboratory. Flight activity was evaluated using a tethered flight-mill. Females had a pre-oviposition period of 3 d, and continued to lay eggs up to 20 d post-emergence. Females flew longest between 17 and 23°C, and 10% of females were capable of continuous long-duration flight even at 12°C. Females flew more actively in their pre-oviposition period, but the females' reproduction period was not clearly separated from their active flight period. The mating status of females had no clear effect on their flight activity. Uptake of honey solution (nectar) was essential for adult survival, and the survival rate was reduced markedly when honey solution was withheld. When honey solution was supplied after 5 d of starvation, the overall longevity of the moths was the same as that of the moths continuously supplied with honey solution since emergence. When honey solution was removed even for 1 d, subsequent flight activity was significantly depressed. Thus, the prediction that the strong propensity for long flights by S. recurvalis may be derived from their dispersal flights to seek flower nectar was not supported from the present experiment.
Public Health Nurse Perceptions of Evolving Work and How Work is Managed: A Qualitative Study.

AIM To explore public health nurse (PHN) perspectives of their evolving work and how PHN work is managed in a Canadian health region. BACKGROUND Professional and public health organizations describe PHN practice roles as population focused work. Healthcare management directs PHN work to achieve specific goals. METHODS In this qualitative study, data were collected during focus groups with 42 PHN participants in one health region. Focus group data were analyzed for meanings and themes. RESULTS PHNs perceived increasing immunizations and limited resources for PHN work meant that population focused care for the public was rationed or missing. Participants perceived the healthcare organization directed, managed, and assigned PHN specialist work, however PHNs managed their client focused practice with knowledge, reasoning, and support from colleagues. CONCLUSIONS Evolving visible PHN work was managed by health organizational management directives to increase immunizations and disease control. PHNs managed their evolving visible and invisible work supported by their knowledge, practice values, and PHN colleagues. IMPLICATIONS FOR NURSING MANAGEMENT Nursing management must lead and communicate the vision supporting better health, better population focused care and health outcomes to PHNs and stakeholders, while reviewing resources needed to optimize public health nursing and improve population health.
Construction of a yrA plasmid for genetic characterization of fluoroquinolone-resistant Staphylococcus aureus

We have constructed a gyrA trans-complementation plasmid, pAT512, by cloning the wild-type gyrA gene of Staphylococcus aurues into expression vector pAT392. Introduction by electrotransformation of pAT512 into a high-level fluoroquinolone resistant mutant of S. aureus (ciprofloxacin MIC= 16 μg ml−1) having a gyrA mutation which results in a Ser-84 to Leu substitution, reduced the MICs of norfloxacin and ciprofloxacin for the host of four- and eight-fold, respectively.
Effect of log position in European Larch (Larix decidua Mill.) tree on the technological properties of particleboard

The impact of log position in European Larch (Larix decidua Mill.) tree on the physical, mechanical, and surface properties of the particleboard was investigated. The logs were divided into five segments from the butt to the top of tree, which were 0–3 m, 3–6 m, 6–9 m, 9–12 m, and 12–15 m, respectively. The fiber length and wall thickness of the wood decreased with the increase in the tree height while the lumen diameter decreased. Similarly, the amount of cellulose and lignin decreased with the increase in tree height while the amount of hemicelluloses increased. The highest solubility values (hot and cold water, NaOH, and alcohol-benzene) and pH of the wood were found in the butt log, followed by the middle log, and top log, respectively. The physical (thickness swelling, water absorption) and mechanical properties (modulus of rupture, modulus of elasticity, and internal bond), and surface quality (surface roughness and contact angle) of the particleboards were negatively affected by increasing tree height. The best properties were obtained for the particleboards produced from the particles of the butt log (0–3 m).
Annual reproductive cycle and annual cycles of reproductive hormones in plasma of female Niveoscincus metallicus (Scincidae) from Tasmania

Niveoscincus metallicus is a small viviparous skink common in Tasmania. We describe the annual reproductive cycle of female N. metallicus and relate this to changes in plasma levels of progesterone and estradiol. Vitellogenesis begins in autumn (March), with first matings in April, prior to entering winter torpor. Ovulation occurs in mid-September about two weeks after spring emergence. Size adjusted atresia of vitellogenic follicles was 8% in the population studied. No loss of embryos in utero was observed and young are born in January. Plasma estradiol levels are significantly elevated during the preovulatory phase. Progesterone concentrations are low through most of the year but rise during gestation and are highest during the second trimester, declining well before parturition.
Neurophysiological impairments in multiple sclerosis—Central and peripheral motor pathways

A systematic review of the literature was conducted comparing neurophysiological outcomes in persons with multiple sclerosis (PwMS) to healthy controls (HC), in studies of the central nervous system (CNS) function comprising motor evoked potentials (MEP) elicited by transcranial magnetic stimulation (TMS) and in studies of the peripheral nervous system (PNS) function comprising electroneuronography (ENG) outcomes elicited by peripheral nerve stimulation. Studies comparing neuromuscular function, assessed during maximal voluntary contraction (MVC) of muscle, were included if they reported muscle strength along with muscle activation by use of electromyography (EMG) and/or interpolated twitch technique (ITT). Studies investigating CNS function showed prolonged central motor conduction times, asymmetry of nerve conduction motor pathways, and prolonged latencies in PwMS when compared to HC. Resting motor threshold, amplitude, and cortical silent periods showed conflicting results. CNS findings generally correlated with disabilities. Studies of PNS function showed near significant prolongation in motor latency of the median nerve, reduced nerve conduction velocities in the tibial and peroneal nerves, and decreased compound muscle action potential amplitudes of the tibial nerve in PwMS. ENG findings did not correlate with clinical severity of disabilities. Studies of neuromuscular function showed lower voluntary muscle activation and increased central fatigue in PwMS, whereas EMG showed divergent muscle activation (ie, EMG amplitude) during MVC. When comparing the existing literature on neurophysiological motor examinations in PwMS and HC, consistent and substantial impairments of CNS function were seen in PwMS, whereas impairments of the PNS were less pronounced and inconsistent. In addition, impairments in muscle activation were observed in PwMS.
Respiratory Mitochondrial Efficiency and DNA Oxidation in Human Sperm after In Vitro Myo-Inositol Treatment

Semen samples are known to contain abnormal amounts of reactive oxygen species (ROS) and oxygen free radicals; therefore, the identification of antioxidant molecules able to counteract the oxidative damage caused by ROS is foresight. Indeed, improving semen quality in terms of motility and reduction in DNA damage, can significantly improve the fertilization potential of sperm in vitro. To this regard, myo-inositol, based on its antioxidant properties, has been reported to be effective in improving sperm quality and motility in oligoasthenozoospermic patients undergoing assisted reproduction techniques when used as a dietary supplementation. Moreover, in vitro treatment demonstrated a direct relationship between myo-inositol, mitochondrial membrane potential and sperm motility. This experimental study aimed to evaluate the effects of myo-inositol (Andrositol-lab) in vitro treatment on sperm motility, capacitation, mitochondrial oxidative phosphorylation and DNA damage. Our results demonstrate that myo-inositol induces a significant increase in sperm motility and in oxygen consumption, the main index of oxidative phosphorylation efficiency and ATP production, both in basal and in in vitro capacitated samples. Moreover, we provide evidence for a significant protective role of myo-inositol against oxidative damage to DNA, thus supporting the in vitro use of myo-inositol in assisted reproductive techniques. Even if further studies are needed to clarify the mechanisms underlying the antioxidant properties of myo-inositol, the present findings significantly extend our knowledge on human male fertility and pave the way to the definition of evidence-based guidelines, aiming to improve the in vitro procedure currently used in ART laboratory for sperm selection.
Factors in B cell competition and immunodominance

The majority of all vaccines work by inducing protective antibody responses. The mechanisms by which the B cells responsible for producing protective antibodies are elicited to respond are not well understood. Interclonal B cell competition to complex antigens, particularly in germinal centers, has emerged as an important hurdle in designing effective vaccines. This review will focus on recent advances in understanding the roles of B cell precursor frequency, B cell receptor affinity for antigen, antigen avidity, and other factors that can substantially alter the outcomes of B cell responses to complex antigens. Understanding the interdependence of these fundamental factors that affect B cell responses can inform current vaccine design efforts for pathogens with complex proteins as candidate immunogens such as HIV, influenza, and coronaviruses.
Efffect of Feeding Probiotic (Lactobacillus cremoris) on Growth and Survival of Macrobrachium rosenbergii Post Larvae

Abstract Suralikar, V. and Sahu, N. P. 2001. Efffect of feeding probiotic (Lactobacillus cremoris) on growth and survival of Macrobrachium rosenbergii post larvae. J. Appl. Anim. Res., 20: 117–124. To study the viability of probiotic (Lactobacillus cremoris) and optimum concentration in the diet, its impact on growth and survival of Macrobrachium rosenbergii post larvae (PL), three hundred PL (140–170 mg) were equally divided in five experimental groups having four replicates each. Five experimental diets prepared by adding different concentrations of L. cremoris (T1, 2.8 × 10 11 cfu/100g; T2, 5.7x 1011 cfu/100g; T3, 8.5 × 10 11 cfu/100g; T4, 11.4 × 10 11 cfu/100g; T5, control) with similar all other ingredients were fed for 60 days. Although insignificant (P<0.05) but growth promoting effect was elucidated at a minimum concentration of 8.5 × 10 11 cfu/100g (L. cremoris) diet. But maximum concentration of inclusion remained inconclusive and needs further investigation. Maximum viability of L. cremoris in the pelleted diet was found to be 18 days at 4 C. Survival was not affected due to feeding of L. cremoris in the diet of Macrobrachium rosenbergii PL.
A Cross-Sectional Exploration of Cytokine–Symptom Networks in Breast Cancer Survivors Using Network Analysis

Purpose The purpose of this study is to (a) visualize the symptom–cytokine networks (perceived stress, fatigue, loneliness, perceived cognitive impairment, daytime sleepiness, sleep quality, and 13 cytokines) and (b) explore centrality metrics of symptom–cytokine networks in breast cancer survivors who completed chemotherapy treatment. Methods Cross-sectional analysis of data collected from 66 breast cancer survivors who were on average three years post chemotherapy completion. Perceived stress, fatigue, loneliness, perceived cognitive impairment, daytime sleepiness, and sleep quality were measured with self-report instruments, and a panel of 13 cytokines was measured from serum using multiplex assays. Symptoms and cytokines were simultaneously evaluated with correlations, network analysis, and community analysis. Results Network analysis revealed the nodes with the greatest degree and closeness were interleukin-2, granulocyte-macrophage colony-stimulating factor, interleukin-13, and perceived cognitive impairment. Node betweenness was highest for perceived cognitive impairment and interleukin-2. Community analysis revealed two separate communities of nodes within the network (symptoms and the cytokines). Several edges connected the two communities including perceived cognitive impairment, stress, fatigue, depression, interleukin-2, granulocyte-macrophage colony-stimulating factor, interleukin-8, interleukin-13, and interleukin-10. Partial correlation analyses revealed significant negative relationships between interleukin-2 and fatigue, loneliness, stress, and perceived cognitive impairment (rs = −.27 to −.37, ps < .05) and a significant negative relationship between perceived cognitive impairment and granulocyte-macrophage colony-stimulating factor (r = −.34, p < .01). Conclusions Our analyses support that perceived cognitive impairment, stress, loneliness, depressive symptoms, and fatigue co-occur and extend the literature by suggesting that interleukin-2 may contribute to the underlying mechanistic pathway of these co-occurring symptoms. Our findings add to a growing body of literature that is shifting to study symptoms as they co-occur, or cluster, rather than individual symptoms.