Miracle-dz/UniFAR-FTUs huggingface dataset:
Streptococcus suis in Brazil: Genotypic, Virulence, and Resistance Profiling of Strains Isolated from Pigs between 2001 and 2016

Streptococcus suis remains an important challenge for the worldwide swine industry. Considering that Brazil is a major pork producer and exporter, proper monitoring of the pathogen and resistance rates are required. We present here the characterization of Brazilian S. suis strains isolated over a 15 year period by pulsed-field gel electrophoresis (PFGE) typing, capsular, virulence, and antimicrobial resistance profiling. Serotype prevalence revealed a predominance of serotype 2/½ followed by 3, 7, 1/14, 6, 8, 18, 28, and 27; the latter had not yet been reported in Brazil. Resistance profiling enabled the differentiation of nine profiles presenting resistance to three and up to eight antimicrobial classes. Even though an association between the most resistant strains and isolation year starting from 2009 was observed, a high frequency of multidrug-resistant strains isolated from 2001 to 2003 was also detected. This suggests that despite the isolation period, S. suis strains already presented high resistance selection pressure. A slight association of serotype 2/½ with some virulence profiles and PFGE pulsotypes was also identified. Nevertheless, no clonal dispersion or persistency of clones over the analyzed years and herds was detected.
Magnetic State Observer in NDT and SHM Studies

The article presents the project for the use of magnetic magnetometers in the 0 500 Hz band for remote, noncontact monitoring of object operation and assessment of the technical condition of ferromagnetic elements and systems. At the beginning, the theoretical foundations of passive magnetic tests and applied magnetometers (multi channels 3 D system) have been presented. Next, selected results of laboratory tests and applied measurement data analysis methods are presented. Then selected results of tests carried out on the real object are presented, among others Kaplan turbine and turbine jet engine. Finally, discussing the results of research, the strengths and weaknesses of the magnetic state observer were pointed out. It has been experimentally demonstrated that a remote magnetic state observer provides qualitative and quantitative diagnostic information that can be used in nondestructive testing (NDT) and structural health monitoring (SHM).
Thought Question: Does environmental ambient noise in high school classrooms make learning more challenging and affect student mathematics achievement? Research Article: Effects of Ambient Noise on the Measurement of Mathematics Achievement for Urban High School Students

Students from urban high schools are usually faced with adverse environmental factors in their pursuit of academic success. These factors make learning more challenging and may confound the measurement of academic performance itself. This study explores how one such factor, ambient noise, affects the measurement of mathematics achievement. Overall, about 40% of students were bothered by noise during testing. The more bothersome the noise is, the lower the math score tends to be. Noise coping explains about 10% of the test score difference, comparable with that by grade point average. These findings indicate a clear association between noise and math achievement measurement.
OMNIDIRECTIONAL STEREOSCOPIC VISION SYSTEMS FOR PLANETARY EXPLORATION ROVERS *

Exploration rovers using stereoscopic cameras to perceive their environment usually need to take several images in different directions to cover all their surroundings, due to the limited field of view (FOV) of conventional cameras. To be able to point the cameras in the desired directions, they need to be mounted on top of a pan-tilt unit (PTU). The PTU is a two-axis gimbal with two motors and its use entails not only an extra mass on the vehicle (typically on top of a mast that is therefore required to be stiffer and thus, heavier), but also additional failure points as well as an increased energy consumption.
Dried Rumen Digesta Pellet Can Enhance Nitrogen Utilization in Thai Native, Wagyu-Crossbred Cattle Fed Rice Straw Based Diets

Simple Summary Generated rumen digesta is wasted and becomes an environmental contaminant in most slaughterhouses in Thailand. Dried rumen digesta (DRD) is a mixture of digestible and indigestible feed residues and are fairly rich in nutrients. DRD has the capacity to become an alternative protein source for ruminants’ diets. DRD in pellet form could be an alternative strategic supplement for Thai-native, Wagyu-crossbred cattle to enhance N balances and microorganisms. Abstract The goal of the current research was to study the effects of a diet of dried rumen digesta pellets (DRDP) on diet utilization, ruminal microorganisms, and ruminal microbes in Thai native, Wagyu-crossbred cattle. Four Thai native, Wagyu-crossbred, beef cattle were assigned to a 4 × 4 Latin square design to supplement DRDP levels at 0, 50, 100, and 150 g/d, respectively. Rice straw intake, total intake, and estimated energy intake varied significantly among the different DRDP levels. Nitrogen intake, apparent N absorption, and apparent N retention were significantly enhanced when compared to the 0 g/d DRDP. DRDP supplementation at 150 g/d produced the greatest apparent digestibility of crude protein compared to the group that was not fed DRDP. Supplementation of DRDP did not alter the population of protozoa, whereas the addition of 150 g DRDP significantly increased the fungal zoospore. Supplementation of DRDP at various levels did not change the concentration of volatile fatty acid (VFA) or the VFA profiles. Thus, DRDP could be an alternative strategic supplement for Thai-native, Wagyu-crossbred cattle in order to enhance N utilization and fungal zoospores.
Modulation of adiponectin receptors AdipoR1 and AdipoR2 by phage display-derived peptides in in vitro and in vivo models

Abstract Type 2 diabetes (T2D) is often linked to metabolic syndrome, which assembles various risk factors related to obesity. Plasma levels of adiponectin are decreased in T2D and obese subjects. Aiming to develop a peptide able to bind adiponectin receptors and modulate their signalling pathways, a 12-amino acid sequence homologous in AdipoR1/R2 has been targeted by phage display with a linear 12-mer peptide library. The selected peptide P17 recognises AdipoR1/R2 expressed by skeletal muscle, liver and pancreatic islets. In HepaRG and C2C12 cells, P17 induced the activation of AMPK (AMPKα-pT172) and the expression of succinate dehydrogenase and glucokinase; no cytotoxic effects were observed on HepaRG cells. In db/db mice, P17 promoted body weight and glycaemia stabilisation, decreased plasma triglycerides to the range of healthy mice and increased adiponectin (in high fat-fed mice) and insulin (in chow-fed mice) levels. It restored to the range of healthy mice the tissue levels and subcellular distribution of AdipoR1/R2, AMPKα-pT172 and PPARα-pS12. In liver, P17 reduced steatosis and apoptosis. The docking of P17 to AdipoR is reminiscent of the binding mechanism of adiponectin. To conclude, we have developed an AdipoR1/AdipoR2-targeted peptide that modulates adiponectin signalling pathways and has therapeutic relevance for T2D and obesity associated pathologies.
The phenomenology of pain in Parkinson’s disease

Background Parkinson’s disease (PD) is a neurodegenerative disorder that is the second most common disorder after Alzheimer’s disease. PD includes both “motor” and “non-motor” symptoms, one of which is pain. The aim of this study was to investigate the clinical characteristics of pain in patients with PD. Methods This cross-sectional study included 250 patients diagnosed with PD, 70% of which had mild to moderate PD (stages 2/3 of Hoehn and Yahr scale). The average age was 67.4 years, and the average duration since PD diagnosis was 7.1 years. Relevant data collected from PD patients were obtained from their personal medical history. Results The prevalence of pain was found to be high (82%), with most patients (79.2%) relating their pain to PD. Disease duration was correlated with the frequency of intense pain (R: 0.393; P < 0.05). PD pain is most frequently perceived as an electrical current (64%), and two pain varieties were most prevalent (2.60 ± 0.63). Our findings confirm links between pain, its evolution over time, its multi-modal character, the wide variety of symptoms of PD, and the female sex. Conclusions Our results demonstrated that the pain felt by PD patients is mainly felt as an electrical current, which contrasts with other studies where the pain is described as burning and itching. Our classification is innovative because it is based on anatomy, whereas those of other authors were based on syndromes.
Association between HLA-DRB1* allele polymorphism and caries susceptibility in Han Chinese children and adolescents in the Xinjiang Uygur Autonomous Region

Objective The rate of caries and the mean number of decayed, missing, or filled teeth were reported to be significantly higher in children in the Xinjiang Uygur Autonomous Region than in children in eastern China. Little is known regarding the genetic basis of caries among residents of the Xinjiang Uygur Autonomous Region. This study investigated the association between HLA-DRB1 alleles and caries susceptibility in Han Chinese children and adolescents in the Xinjiang Uygur Autonomous Region. Methods HLA-DRB1 allele frequency was assessed in DNA samples from buccal swabs of 42 patients with caries and 123 healthy control participants using a polymerase chain reaction method with sequence-specific primers. The chi-squared test or Fisher’s exact test, followed by Bonferroni correction, was used to calculate differences in allele frequencies between groups. Results Compared with the healthy controls, the allele frequency of HLA-DRB1*13 was significantly higher in patients with caries in the Xinjiang Uygur Autonomous Region (35.71% vs. 18.70%). The allele frequency of HLA-DRB1*09 was significantly lower in patients with caries than in healthy controls (4.76% vs. 25.20%). Conclusions HLA-DRB1*13 alleles could confer greater caries susceptibility, whereas HLA-DRB1*09 could be protective against caries pathogenesis, in Han Chinese children in the Xinjiang Uygur Autonomous Region.
Overweight in Young Athletes: New Predictive Model of Overfat Condition

The aim of the study is to establish a simple and low-cost method that, associated with Body Mass Index (BMI), differentiates overweight conditions due to a prevalence of lean mass compared to an excess of fat mass during the evaluation of young athletes. 1046 young athletes (620 male, 426 female) aged between eight and 18 were enrolled. Body composition assessments were performed with anthropometry, circumferences, skinfold, and bioimpedance. Overweight was established with BMI, while overfat was established with the percentage of fat mass: 3.5% were underweight, 72.8% were normal weight, 20.1% were overweight, and 3.5% were obese according to BMI; according to the fat mass, 9.5% were under fat, 63.6% were normal fat, 16.2% were overfat, and 10.8% were obese. Differences in overfat prediction were found using BMI alone or with the addition of the triceps fold (area under the receiver operating characteristics curve (AUC) for BMI = 0.867 vs. AUC for BMI + TRICEPS = 0.955, p < 0.001). These results allowed the creation of a model factoring in age, sex, BMI, and triceps fold that could provide the probability that a young overweight athlete is also in an overfat condition. The calculated probability could reduce the risk of error in establishing the correct weight status of young athletes.
‘Even peacekeepers expect something in return’: A qualitative analysis of sexual interactions between UN peacekeepers and female Haitians

ABSTRACT The UN maintains a zero-tolerance policy on sexual interactions between peacekeepers and beneficiaries of assistance. Our research describes the lived experience of engaging sexually with UN peacekeepers during Mission des Nations Unies pour la Stabilisation en Haiti (MINUSTAH) from the perspectives of Haitian women/girls. Eighteen semi-structured interviews were conducted with Haitian women raising children fathered by MINUSTAH peacekeepers. Transcripts were analyzed according to empirical phenomenology. Adverse socio-economic conditions were key contextual factors. Three themes related to the nature of the sexual interactions emerged: sexual violence, transactional sex, and long-term transactional relationships imbedded in perceptions of love. Most sexual interactions were transactional and nuanced since the peacekeeper assumed the role of romantic and material provider. Sexual consent was conceptualised as the ability to weigh the benefits and consequences of engaging sexually with peacekeepers. Sexual violence was identified among minors and in instances of sexual abuse. This study provides empirical evidence to support a nuanced understanding of sexual relationships between women/girls and peacekeepers. In addition to holding peacekeepers accountable, a harm-reduction approach that aims to raise awareness for peacekeeping codes of conduct and provide comprehensive reproductive and sexual education should be considered.
Sacituzumab govitecan, an antibody‐drug conjugate targeting trophoblast cell‐surface antigen 2, shows cytotoxic activity against poorly differentiated endometrial adenocarcinomas in vitro and in vivo

Endometrial cancer is the most common gynecologic malignancy in developed countries. The antibody–drug conjugate (ADC) sacituzumab govitecan (SG) targets trophoblast cell‐surface antigen‐2 (Trop‐2) – a cell‐surface glycoprotein highly expressed in many epithelial tumors – and delivers the active metabolite of irinotecan SN‐38 to Trop‐2‐positive tumor cells. We evaluated Trop‐2 expression in endometrial endometrioid carcinoma (EC) tissues and the activity of SG against primary poorly differentiated EC cell lines and xenografts. Trop‐2 expression was assessed in 143 formalin‐fixed–paraffin‐embedded tumors and seven primary tumor cell lines by immunohistochemistry and flow cytometry, respectively. Cell viability of primary tumor cell lines was assessed following exposure to SG, or control antibodies. Antibody‐dependent cell cytotoxicity (ADCC) against Trop‐2‐positive and Trop‐2‐negative EC cell lines was measured in vitro using 4‐h chromium release assays. A Trop‐2‐positive EC xenograft model was used to determine the in vivo activity of SG. Moderate‐to‐strong staining was detected in 84% (120/143) of EC samples, whereas 43% (3/7) of the primary EC cell lines tested overexpressed Trop‐2. EC cell lines overexpressing Trop‐2 were significantly more sensitive to SG compared to control ADC (P = 0.014 and P = 0.005). Both SG and the unconjugated parental antibody hRS7 mediated high ADCC against Trop‐2‐positive cell lines. Moreover, SG induced significant bystander killing of Trop‐2‐negative tumors cocultured with Trop‐2‐positive tumors. In the xenograft model, intravenous administration of SG twice weekly for three weeks was well tolerated and demonstrated impressive tumor growth inhibition against poorly differentiated, chemotherapy‐resistant EC xenografts (P = 0.011). In summary, SG is a novel ADC with remarkable preclinical activity against poorly differentiated EC cell lines overexpressing Trop‐2. These findings warrant future clinical trials.
Hypertrophic Cardiomyopathy: An Overview of Genetics and Management

Hypertrophic cardiomyopathy (HCM) is a genetically heterogeneous cardiac muscle disorder with a diverse natural history, characterized by unexplained left ventricular hypertrophy (LVH), with histopathological hallmarks including myocyte enlargement, myocyte disarray and myocardial fibrosis. Although these features can cause significant cardiac symptoms, many young individuals with HCM are asymptomatic or mildly symptomatic. Sudden cardiac death (SCD) may occur as the initial clinical manifestation. Over the past few decades, HCM has been considered a disease of sarcomere, and typically as an autosomal dominant disease with variable expressivity and incomplete penetrance. Important insights into the genetic landscape of HCM have enhanced our understanding of the molecular pathogenesis, empowered gene-based diagnostic testing to identify at-risk individuals, and offered potential targets for the development of therapeutic agents. This article reviews the current knowledge on the clinical genetics and management of HCM.
Microstructure and Mechanical Properties of Al-12Si Alloys Fabricated by Ultrasonic-Assisted Laser Metal Deposition

This paper presents a method of ultrasonic-assisted laser metal deposition of Al-12Si alloy. The effects of the ultrasonic power and remelting treatment on the development of porosity, microstructural evolution, and tensile properties of the deposits were investigated. The results suggested that a combination of an ultrasonic vibration and remelting treatment could prolong the existence of the molten pool and the effect of the ultrasound. Therefore, the density of the samples increased from 95.4% to 99.1% compared to the as-prepared samples. The ultrasonic action in the molten pool could not only increase the density of the samples but also refine the grains and improve the tensile properties of the samples. Metallographic observation showed that the maximum size of the primary α-Al dendrites were refined from 277.5 µm to 87.5 µm. The ultimate tensile strength and elongation of the remelting treatment samples with ultrasonic vibration were ~227 ± 3 MPa and 12.2% ± 1.4%, respectively, which were approximately 1.17 and 1.53 times those of the as-prepared samples, respectively. According to the tensile properties and fracture analysis, the density increase dominated the improvement of the mechanical properties.
Small RNAs and extracellular vesicles: New mechanisms of cross-species communication and innovative tools for disease control

1 Department of Microbiology and Plant Pathology, Center for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, California, United States of America, 2 Department of Biology, Ludwig-Maximilians University of Munich (LMU), Munich, Germany, 3 Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom, 4 Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom
Electrical stimulation of the ventral tegmental area evokes sleep‐like state transitions under urethane anaesthesia in the rat medial prefrontal cortex via dopamine D1‐like receptors

The role of dopamine in regulating sleep‐state transitions during, both natural sleep and under anaesthesia, is still unclear. Recording in vivo in the rat mPFC under urethane anaesthesia, we observed predominantly slow wave activity (SWA) of <1 Hz in the local field potential interrupted by occasional spontaneous transitions to a low‐amplitude‐fast (LAF) pattern of activity. During periods of SWA, transitions to LAF activity could be rapidly and consistently evoked by electrical stimulation of the ventral tegmental area (VTA). Spontaneous LAF activity, and that evoked by stimulation of the VTA, consisted of fast oscillations similar to those seen in the rapid eye movement (REM)‐like sleep state. Spontaneous and VTA stimulation‐evoked LAF activity occurred simultaneously along the dorsoventral extent of all mPFC subregions. Evoked LAF activity depended on VTA stimulation current and could be elicited using either regular (25–50 Hz) or burst stimulation patterns and was reproducible upon repeated stimulation. Simultaneous extracellular single‐unit recordings showed that during SWA, presumed pyramidal cells fired phasically and almost exclusively on the Up state, while during both spontaneous and VTA‐evoked LAF activity, they fired tonically. The transition to LAF activity evoked by VTA stimulation depended on dopamine D1‐like receptor activation as it was almost completely blocked by systemic administration of the D1‐like receptor antagonist SCH23390. Overall, our data demonstrate that activation of dopamine D1‐like receptors in the mPFC is important for regulating sleep‐like state transitions.
Calcium Intake from Food and Supplemental Sources Decreased in the Canadian Population from 2004 to 2015.

BACKGROUND In light of the publications (2008-2014) linking calcium intake, mainly from supplements, to the elevated risk of cardiovascular events, there is a need to determine if there have been any changes in usual intakes of calcium among Canadians. OBJECTIVE We aimed to examine changes in dietary and total usual intake of calcium among Canadians aged ≥1 y over the period 2004-2015. METHODS We used nutrition data from 2 nationally representative surveys conducted in 2004 and 2015 (CCHS 2004 Cycle 2.2 and CCHS-Nutrition 2015). This study included all the Canadians across 10 provinces, and the analyses were performed across different age/sex groups. We used the National Cancer Institute (NCI) method to estimate the usual intake of calcium and the prevalence of calcium inadequacy. Multiple logistic regression was performed to assess the relation between supplement uses and sociodemographic variables. RESULTS From 2004 to 2015, the usual intake of calcium from food sources significantly decreased in calcium supplement nonusers (from 872 ± 18.2 mg/d to 754 ± 18.0 mg/d), but not in calcium supplement users. The contribution of calcium from the Milk and alternatives food group significantly decreased by 7.5% and 6.1% in calcium supplement users and nonusers, respectively. The prevalence of calcium supplement use significantly decreased from 2004 to 2015 in the Canadian population, from 27.5% to 22.0%. During this time, the percentage contribution of calcium from supplemental sources significantly decreased among Canadians, especially women. The prevalence of calcium inadequacy increased from 58.0% to 68.0% in supplement nonusers; however, among users of calcium supplements, the prevalence of calcium inadequacy remained at ∼31%. CONCLUSIONS Calcium intake from both food and supplemental sources decreased in the Canadian population over an 11-y period, which must be addressed by policy-makers in their efforts to decrease the high prevalence of calcium inadequacy.
Finite-Time Attitude Stabilization Adaptive Control for Spacecraft with Actuator Dynamics

For the attitude stabilization of spacecraft with actuator dynamics, this paper proposed a finite-time control law. Firstly, the dynamic property of the actuator is analyzed by an example. Then, a basic control law is derived to achieve the finite-time stability using the double fast terminal sliding mode manifold. When there is no prior knowledge of time matrix of the actuator, an adaptive law is proposed to estimate the unknown information. An adaptive control law is derived to guarantee the finite-time convergence of the attitude, and a Lyapunov-based analysis is provided. Finally, simulations are carried out to demonstrate the effectiveness of the proposed control law to the attitude stabilization with the actuator dynamics. The results show that the high-precision attitude control performance can be achieved by the proposed scheme.