New Rules for Club Development: New Insights into Human Small Airway Epithelial Club Cell Ontogeny and Function.

The human airways are lined with a pseudostratified epithelium comprised of a number of distinct populations of cells with specialized effector functions (1, 2). These include ciliated cells, secretory cells (goblet and club), basal cells, and neuroendocrine cells, although the abundance and prevalence of each distinct population vary considerably throughout the proximal–distal axis of the airways and also exhibit significant interspecies differences. Pathological airway remodeling is a prominent feature of many chronic lung diseases, including chronic obstructive pulmonary disease, asthma, and cystic fibrosis, and encompasses substantial pathological alterations in the airway epithelium. However, the etiology of these pathological changes is poorly understood, owing in part to a lack of basic knowledge regarding the mechanisms that regulate differentiation and repair of these epithelial populations. Accordingly, elucidating the specific roles of resident stem cells or progenitors that are responsible for differential postnatal growth, maintenance of homeostasis, and regeneration of the airway epithelium is of fundamental importance (3). It is clear that the lungs are capable of intrinsic repair, and, given the right circumstances, this opens up the possibility of designing and implementing regenerative medicine strategies to repair lung damage across a broad range of diseases. constitute Club cells represent the major secretory cells of the smallairway epithelium in humans, constituting approximately 20% of such cells, and are clearly distinguishable morphologically by their smooth, dome-shaped apical surface that extends into the lumen, and their expression of SCG1A1 protein (also known as club cell 10) (4). Much of our knowledge concerning the ontogeny and function of these specialized epithelial cells originates from studies conducted in mice; however, there are substantial anatomical differences between human and murine airways that complicate translatability (5). Importantly, basal cells, a recognized stem cell population in both mouse and human lungs, are only present in the trachea of mouse lungs, whereas in humans, the pseudostratified epithelium containing basal cells extends much farther down the respiratory tree, as far as the respiratory bronchioles; thus, the mouse trachea more closely resembles the smaller airways seen in humans. Furthermore, club cells line all conducting airways of the murine lung, but are restricted to just the small airways in humans. In the pseudostratified epithelium of the mouse trachea, basal cells act as the primary progenitor cells, capable of self-renewal and differentiation into club and ciliated cells (6–8). The club cells of the mouse trachea exist as a transiently amplifying population, but their capacity for selfrenewal and multilineage differentiation is enhanced after injury (6). Conversely, a self-renewing population of club cells maintain the basal cell–deficient epithelium of the distal bronchial and bronchiolar airways in mice, functioning as progenitors to ciliated cells and mucin-secreting goblet cells (6, 9). Furthermore, studies in mice have highlighted that club cells play important protective roles by participating in immune modulation, oxidative stress reduction, and xenobiotic metabolism (10–13). However, given the marked differences in the composition of cells lining the mouse and human airways, it is vital that we clarify the origin and roles of human airway club cells in homeostasis and repair before we move further along the translational pathway. Although basal cells are progenitors of ciliated and mucus-producing cells in human airways (8, 14), the ontogeny and function of human small airway club cells are unknown, and this is the subject of the elegant study reported by Zuo and colleagues (pp. 1375–1388) in this issue of the Journal (15). The authors used principal component gradient analysis to demonstrate an ontological link between small airway basal cells and club cells in healthy nonsmokers, and subsequently showed that isolated basal cells differentiated into club cells in air–liquid interface (ALI) cultures. Thus, in keeping with findings from the mouse trachea, basal cells were convincingly shown to be a progenitor to club cells under steady-state conditions in human small airways. Although this conclusion is compelling, it should be acknowledged that the in vitro culture and differentiation of basal cells into a pseudostratified epithelium at the ALI may never truly reflect the complex microenvironmental cues and interactions that regulate airway epithelial homeostasis in the in vivo context of the human lung, as epitomized by the overrepresentation of basal cells seen in the ALI cultures. Nor does it preclude the potential for club cell self-renewal, as seen in mice and supported by previous observations of club cell proliferation in human small airways (4). Furthermore, given studies in mice, it would be prudent to question whether the relative significance of basal cells as club cell progenitors during postnatal lung growth is different from that observed after lung injury. Are club cells that arise from basal cells functionally the same as those that arise from existing club cells, and is differentiation aberrant or subverted in chronic lung disease? As discussed above, studies in mice demonstrated that club cells not only self-renew but also have the capacity to differentiate into multiple mature epithelial cell types, including ciliated and mucus-producing cells (6, 9). Accordingly, it is intriguing that transcriptome and ALI culture analyses defined subsets of Originally Published in Press as DOI: 10.1164/rccm.201805-0925ED on June 7, 2018