Miracle-dz/UniFAR-FTUs huggingface dataset:
Sperm DNA damage in potentially fertile homozygous beta-thalassaemia patients with iron overload.

BACKGROUND To test the hypothesis that human sperm DNA could sustain iron-induced oxidative damage and reduce its fertilizing ability, we studied patients with homozygous beta-thalassaemia major (HbTh) as a model of iron overload. METHODS Sperm from six thalassaemic patients and five age-matched controls were assessed by the sperm chromatin structure assay (SCSA) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) assay. Semen parameters, endocrine markers of testicular function, iron profiles and the presence of organ dysfunction were also determined. RESULTS All patients with HbTh were iron overloaded (median ferritin: 2251 microg/l) and had evidence of spontaneous spermatogenesis. Thalassaemic patients had more sperm DNA damage than the controls (P < 0.01). The sperm DNA damage by SCSA and TUNEL were positively correlated (P < 0.05). Sperm motility and TUNEL results were negatively correlated (P < 0.05), while the age of onset of chelation and sperm DNA damage were positively associated with both SCSA (R(2) = 0.80, P = 0.016) and TUNEL data (R(2) = 0.67, P < 0.044). No other biochemical or clinical data were associated with sperm DNA damage. CONCLUSIONS The increase in sperm DNA damage and the negative correlation between sperm motility and DNA damage suggest that iron overload in HbTh predisposes sperm to oxidative injury. This finding has important implications in assisted reproductive procedures such as ICSI where there is increased risk of transmitting defective DNA to the offspring.
Managing customer knowledge through the use of big data analytics in tourism research

ABSTRACT The use of big data is growing in relevance and importance in tourism management research. Companies operating in this industry are exploiting big data analytics and developing systems to manage customer knowledge and provide the best service in the right place at the right time. This paper aims to provide a systematic literature review to present issues associated with the use of big data in tourism and identify future research directions on the topic. To achieve this aim, this paper develops a citation network analysis methodology to drive the content analysis and explore the content of 109 selected papers. The findings of this review highlight that although there is an increasing number of contributions on the topic, there are yet some issues that require to be further developed. In particular, the paper identifies research gaps and consequent research questions that represent an agenda for both researchers and practitioners.
Tracing the roots of early sexual debut among adolescents in psychiatric care.

OBJECTIVE To identify the most important social and personal characteristics related to early sexual debut among troubled teenagers. METHOD One hundred ninety-eight youths aged 12-19 years were recruited from outpatient mental health clinics and completed self-reports and interviews about their age of sexual debut; family, peer, and partner relationships (e.g., parental hostile control, negative peer influence, need for intimacy); and personal characteristics (e.g., achievement motivation, externalizing problems). Broad-band (externalizing, internalizing) and narrow-band (depression/anxiety, delinquency) psychopathology was assessed with the Youth Self-Report and Child Behavior Checklist. RESULTS Optimal Data Analysis was used to generate a classification tree model to identify variables associated with whether or not youths initiated oral, vaginal, and/or anal sexual activity before or after age 14. Three social context variables (parental hostile control, negative and positive peer influence) and one personal characteristic (externalizing problems) correctly classified 87.4% of teenagers as initiating sexual activity at < or =14 or >14 years of age. CONCLUSIONS Parental behavior and peer influence were the most important variables associated with the timing of sexual debut. Results support a social-personal framework for understanding sexual risk-taking among adolescents in psychiatric care, and the data offer relatively strong evidence that specific factors could be used to identify troubled teens at risk for early sexual debut.
9‐cis‐retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF‐I expression in cumulus‐granulosa cells

The isomer 9‐cis of retinoic acid (9‐cis‐RA) exerts a beneficial effect on bovine in vitro development when added to in vitro maturation (IVM) culture. In the present work, 9‐cis‐RA 5 nM was found to be stimulatory as opposed to 500 nM (toxic). Cumulus–oocyte complexes (COCs) were treated with the found physiological dose 9‐cis‐RA 5 nM, and the next determinations performed: (1) relative expression of midkine (MK) and IGF‐I, by reverse transcriptase‐polymerase chain reaction (RT‐PCR), in cumulus‐granulosa cells detached from oocytes; (2) cytoplasmic granular migration, by labeling of oocytes with fluoroscein isothiocyanate lectins; and (3) in vitro survival of blastocysts after vitrification and warming. Gene expression of MK was enhanced by 9‐cis‐RA, but not by 1% ethanol (vehicle). However, we did not detect IGF‐I expression, both in dependence on or in the absence of 9‐cis‐RA acting on cumulus‐granulosa cells. The ability of vitrified blastocysts to survive in vitro was not improved by 9‐cis‐RA. Nevertheless, since only blastocysts obtained from oocytes matured with serum survived, more factors should be considered when evaluating cryopreservation survival. The complete granular migration observed in oocytes matured with 9‐cis‐RA anticipates the gain in developmental competence of the oocyte, being MK probably involved in this beneficial effect. Mol. Reprod. Dev. 66: 247–255, 2003. © 2003 Wiley‐Liss, Inc.
Occurrence and predictors of left ventricular systolic dysfunction at hospital discharge and in long-term follow-up after acute myocardial infarction treated with primary percutaneous coronary intervention.

BACKGROUND Post-ST-segment elevation myocardial infarction (STEMI) left ventricular systolic dysfunction (LVSD) has been identified as an important marker of poor prognosis. AIM To assess the prevalence and course of LVSD at hospital discharge and in long-term follow-up in STEMI patients treated with primary percutaneous coronary intervention (pPCI). METHODS We enrolled 205 patients (157 male, 48 female) with a first STEMI. Echocardiography was performed before hospital discharge and 12 months after STEMI. Left ventricular systolic function (LVSF) parameters were assessed: left ventricular ejection fraction (LVEF), wall motion score index (WMSI), and average peak systolic mitral annular velocity (S') by tissue Doppler echocardiography (TDE). B-type natriuretic peptide plasma concentration was measured at admission (BNP(admission)) and at discharge (BNP(discharge)). RESULTS We found moderate LVSD, both at hospital discharge and after 12 months. Significant global LVSD (LVEF ≤ 40%) was observed in 34% of patients at discharge, and 21% after 12 months (p 〈 0.001). Significant regional LVSD (WMSI ≥ 1.7) after 12 months was less frequent than at discharge (21% vs 33%; p 〈 0.001). More patients had significant longitudinal LVSD (S' ≤ 6.0 cm/s) after 12 months compared to discharge (28% vs 23%; p 〈 0.001). Severe global LVSD (LVEF ≤ 30%) was rare. Univariate logistic regression analysis revealed the predictors of significant global LVSD at 12 months after STEMI to be: anterior location of STEMI; pre-discharge echocardiographic parameters of LVSF and left ventricle size and mass; prepPCI angiographic indices; ratio of the difference of BNP(discharge) and BNPa(dmission) to BNP(admission) expressed as % (BNP(delta) %); time from onset of pain to balloon, and the use of abciximab. Multivariate logistic regression analysis found independent predictors of significant global LVSD at 12 months to be: BNP(delta) % and LVEF at discharge with optimal cut-off values of 728.2% for BNP(delta) % and 37% for LVEF. CONCLUSIONS Patients with a first STEMI treated with pPCI present moderate LVSD, both at hospital discharge and after 12 months. In long-term follow-up, we found an improvement in global LVSF, and, albeit a smaller, improvement in regional LVSF. No improvement in longitudinal LVSF was observed. The increase of BNP during hospitalisation, and LVEF at discharge, are independent predictors of significant global LVSD at 12 months after a first STEMI treated with pPCI. Pre-discharge peak systolic mitral annular velocity obtained by TDE may be useful in predicting LVEF in long-term follow-up in this group of patients.
Bacterial species identification after DNA amplification with a universal primer pair.

The diagnosis of bacterial infections can be difficult and time consuming. Rapid and reliable molecular triage of potentially infected patients, particularly the young and the elderly, would prevent unnecessary hospitalizations, reduce associated medical costs, and improve the quality of care. Polymerase chain reaction (PCR) amplification utilizing a universal bacterial primer pair, followed by hybridization with species-specific probes, would allow rapid identification of the presence or absence of bacterial DNA, along with an identification of the bacterial species present. Molecular microbiological analyses will require access to bacterial strain standards that can be catalogued and distributed to clinical laboratories. We amplified template DNA in filter paper spots containing boiled bacteria from 14 clinical isolates using a universal primer pair for the 16S ribosomal RNA (rDNA) coding sequence. Species-specific probes were hybridized to the amplification products for bacterial species identification. We conclude that template DNA can be identified with species-specific probes after universal bacterial amplification with a single primer pair. We also demonstrate a rapid and efficient method for the long-term storage and cataloguing of bacterial DNA for use in quality control at clinical laboratories adopting molecular diagnostic methodologies. We speculate that PCR amplification combined with species-specific probe hybridization not only will represent an improvement over culture-based methods in terms of speed, sensitivity, and cost, but will also allow for the identification of unculturable bacteria and emerging or reemerging pathogenic organisms.
3-6 junction photovoltaic cells for space and terrestrial concentrator applications

Multi-junction solar cells made from III-V compound semiconductors are the highest efficient photovoltaic devices today. Different solar cell structures have been developed for space as well as terrestrial concentrator applications in a close collaboration between the Fraunhofer ISE and RWE-SSP in Germany. Efficiencies up to 29.1% (AM0) have been recently achieved for a 30.2 cm/sup 2/ GaInP/GaInAs/Ge device at RWE-SSP. Triple-junction solar cells with remaining factors up to 88 % after irradiation with 1 MeV electrons at a fluence of 10/sup 15/ cm/sup -2/ have been demonstrated at Fraunhofer ISE. Multi-junction solar cells with 5 and even 6 junctions for even higher radiation hardness are under development. On the other hand lattice mismatched triple-junction solar cells are an excellent solution for terrestrial concentrator applications. Efficiencies up to 35.2 % at a concentration ratio of 443-637 (AM1.5d, low AOD) have been obtained.
Proteomic profiling of cerebrospinal fluid identifies biomarkers for amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is characterized by degeneration of motor neurons. We tested the hypothesis that proteomic analysis will identify protein biomarkers that provide insight into disease pathogenesis and are diagnostically useful. To identify ALS specific biomarkers, we compared the proteomic profile of cerebrospinal fluid (CSF) from ALS and control subjects using surface‐enhanced laser desorption/ionization‐time of flight mass spectrometry (SELDI‐TOF‐MS). We identified 30 mass ion peaks with statistically significant (p < 0.01) differences between control and ALS subjects. Initial analysis with a rule‐learning algorithm yielded biomarker panels with diagnostic predictive value as subsequently assessed using an independent set of coded test subjects. Three biomarkers were identified that are either decreased (transthyretin, cystatin C) or increased (carboxy‐terminal fragment of neuroendocrine protein 7B2) in ALS CSF. We validated the SELDI‐TOF‐MS results for transthyretin and cystatin C by immunoblot and immunohistochemistry using commercially available antibodies. These findings identify a panel of CSF protein biomarkers for ALS.
An age of deference

THE SOCIAL ORDER At the beginning of the great treatise on the laws and customs of England known as Bracton , the anonymous early thirteenth-century author attempted a classification of persons. His first and most basic division is between the free and the unfree, freedom being ‘the natural power of every man to do what he pleases, unless forbidden by law or force’. A second division is between male, female and hermaphrodite. Yet another classification is between those who are in their own power ( potestas ) and those who are in the power of another. This power may be paternal, that is to say the power of the father, or it may be seigniorial power, which Bracton regards here as essentially power over bondmen (the unfree). This division is complicated by the fact that there are also people who may be said to be in the care rather than the power of others, and by wives, who are ‘under the rod’. These, then, are the basic divisions of mankind. But Bracton highlights a further difference. There are some men of great eminence who rule over others. In spiritual matters these are the pope and his archbishops, bishops and other lesser prelates. In temporal matters they are the king, earls and barons, magnates or vavasours – men, that is, ‘of great dignity’ – and knights.
Appliance usage prediction using a time series based classification approach

Energy management for residential homes and offices require the prediction of the usage(s) or service request(s) of different appliances present in the house. The hardware requirement is more simplified and practical if the task is only based on energy consumption data and no other sensors are used. The proposed model tries to formalize such an approach using a time-series based multi-label classifier which takes into account correlation between different appliances among other factors. In this work, prediction results are shown for 1-hour in the future but this approach can be extended to predict more hours in the future as per the requirement(with restrictions). The learned models and decision tree showing the important factors in the input information is also discussed.
Fatty Acid Regulation of Gene Transcription

ABSTRACT Dietary fat has a dual role in human physiology: a) it functions as a source of energy and structural components for cells; b) it functions as a regulator of gene expression that impacts lipid, carbohydrate, and protein metabolism, as well as cell growth and differentiation. Fatty acid effects on gene expression are cell-specific and influenced by fatty acid structure and metabolism. Fatty acids interact with the genome through several mechanisms. They regulate the activity or nuclear abundance of several transcription factors, including PPAR, LXR, HNF-4, NFκB, and SREBP. Fatty acids or their metabolites bind directly to specific transcription factors to regulate gene transcription. Alternatively, fatty acids indirectly act on gene expression through their effects on a) specific enzyme-mediated pathways, such as cyclooxygenase, lipoxygenase, protein kinase C, or sphingomyelinase signal transduction pathways; or b) pathways that involve changes in membrane lipid/lipid raft composition that affect G-protein receptor or tyrosine kinase-linked receptor signaling. Further definition of these fatty acid-regulated pathways will provide insight into the role dietary fat plays in human health and the onset and progression of several chronic diseases, like coronary artery disease and atherosclerosis, dyslipidemia and inflammation, obesity and diabetes, cancer, major depressive disorders, and schizophrenia.
Signaling pathways crucial for craniofacial development revealed by endothelin-A receptor-deficient mice.

Most of the bone and cartilage in the craniofacial region is derived from cephalic neural crest cells, which undergo three primary developmental events: migration from the rhombomeric neuroectoderm to the pharyngeal arches, proliferation as the ectomesenchyme within the arches, and differentiation into terminal structures. Interactions between the ectomesenchymal cells and surrounding cells are required in these processes, in which defects can lead to craniofacial malformation. We have previously shown that the G-protein-coupled endothelin-A receptor (ET(A)) is expressed in the neural crest-derived ectomesenchyme, whereas the cognate ligand for ET(A), endothelin-1 (ET-1), is expressed in arch epithelium and the paraxial mesoderm-derived arch core; absence of either ET(A) or ET-1 results in numerous craniofacial defects. In this study we have attempted to define the point at which cephalic neural crest development is disrupted in ET(A)-deficient embryos. We find that, while neural crest cell migration in the head of ET(A)(-/-) embryos appears normal, expression of a number of transcription factors in the arch ectomesenchymal cells is either absent or significantly reduced. These ET(A)-dependent factors include the transcription factors goosecoid, Dlx-2, Dlx-3, dHAND, eHAND, and Barx1, but not MHox, Hoxa-2, CRABP1, or Ufd1. In addition, the size of the arches in E10.5 to E11.5 ET(A)(-/-) embryos is smaller and an increase in ectomesenchymal apoptosis is observed. Thus, ET(A) signaling in ectomesenchymal cells appears to coordinate specific aspects of arch development by inducing expression of transcription factors in the postmigratory ectomesenchyme. Absence of these signals results in retarded arch growth, defects in proper differentiation, and, in some mesenchymal cells, apoptosis. In particular, this developmental pathway appears distinct from the pathway that includes UFD1L, implicated as a causative gene in CATCH 22 patients, and suggests parallel complementary pathways mediating craniofacial development.
Differentiation Strategy, R&D Intensity, and Sustainability of Accounting Earnings: With a Focus on Biotech Firms

In this paper, we examine the association between management strategy and a firm’s sustainability of accounting earnings over almost 20,000 firm-year observations in South Korea between 2000 and 2017. Using the differentiation strategy developed by Porter for measuring management strategy, we find that firms that implement the differentiation strategy make more-sustainable accounting earnings, as measured by the persistence of return on assets. In addition, regardless of the magnitude of R&D investments, proper implementation of the differentiation strategy is positively associated with the sustainability of accounting outcomes. However, except when sales increase, a reduction in R&D intensity has a negative impact on the persistence of accounting income for biotech firms. This is because biotech firms are not be able to continue to strengthen core competencies and thus cannot ultimately implement a differentiating strategy effectively.
Evaluation of the antifungal activities of various extracts from Pistacia atlantica Desf

Background and Purpose: Despite the availability of various treatments for fungal diseases, there are some limitations in the management of these conditions due to multiple treatment-related side-effects. The present study was designed to investigate the antifungal properties of different extracts from Pistacia atlantica Desf. Materials and Methods: Different parts of P. atlantica (i.e., dried fruit, fresh fruit and dried leaf) were separately extracted via percolation method with 80% methanol and water. Gas chromatography/mass spectrometry (GC/MS) analysis was performed to determine the main constituents of leaf and fruit extracts from P. atlantica. In vitro anti-Candida activities of the extracts against Candida albicans, Candida glabrata and Saccharomyces cerevisiae were studied. For this purpose, the minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) were determined, using broth microdilution method, according to the modified M27-A3 protocol on yeasts, proposed by the Clinical and Laboratory Standards Institute (CLSI). Results: Based on GC/MS analysis, the main constituents of P. atlantica fruit extracts were β-myrcene (41.4%), α-pinene (32.48%) and limonene (4.66%), respectively, whereas the major constituents of P. atlantica leaf extracts were trans-caryophyllene (15.18%), α-amorphene (8.1%) and neo-allo-ocimene (6.21%), respectively. As the findings indicated, all the constituents exhibited both fungistatic and fungicidal activities, with MICs ranging from 6.66 to 26.66 mg/mL and MFCs ranging from 13.3 to 37.3 mg/mL, respectively. Among the evaluated extracts, the methanolic fresh fruit extract of P. atlantica was significantly more effective than other extracts (P<0.05). Conclusion: Based on the findings of the present study, novel antifungal agents need to be developed, and use of P. atlantica should be promoted in the traditional treatment of Candida infections.
The Glutathione/Glutaredoxin System Is Essential for Arsenate Reduction in Synechocystis sp. Strain PCC 6803

ABSTRACT Arsenic resistance in Synechocystis sp. strain PCC 6803 is mediated by an operon of three genes in which arsC codes for an arsenate reductase with unique characteristics. Here we describe the identification of two additional and nearly identical genes coding for arsenate reductases in Synechocystis sp. strain PCC 6803, which we have designed arsI1 and arsI2, and the biochemical characterization of both ArsC (arsenate reductase) and ArsI. Functional analysis of single, double, and triple mutants shows that both ArsI enzymes are active arsenate reductases but that their roles in arsenate resistance are essential only in the absence of ArsC. Based on its biochemical properties, ArsC belongs to a family that, though related to thioredoxin-dependent arsenate reductases, uses the glutathione/glutaredoxin system for reduction, whereas ArsI belongs to the previously known glutaredoxin-dependent family. We have also analyzed the role in arsenate resistance of the three glutaredoxins present in Synechocystis sp. strain PCC 6803 both in vitro and in vivo. Only the dithiolic glutaredoxins, GrxA (glutaredoxin A) and GrxB (glutaredoxin B), are able to donate electrons to both types of reductases in vitro, while GrxC (glutaredoxin C), a monothiolic glutaredoxin, is unable to donate electrons to either type. Analysis of glutaredoxin mutant strains revealed that only those lacking the grxA gene have impaired arsenic resistance.
Does air pollution decrease inbound tourist arrivals? The case of Beijing

ABSTRACT Tourism industry might be affected by pollution, discouraging current tourism and damaging destination’s image, which compromises future arrivals. This paper quantifies the impact of air pollution on inbound tourism analyzing the case of Beijing city, China. Applying a standard tourism demand function, short-run and long-run impacts are explored in a VECM framework. Results show that air pollution had negative effects on tourist arrivals in the long-run, but not in the short-run. Also, there is a long-run causal relation between air pollution and inbound arrivals from most source countries, while air pollution does not Granger-cause a short-run tourist arrivals decrease.