Miracle-dz/UniFAR-FTUs huggingface dataset:
Role of cyclooxygenase‐1 and ‐2 in endothelium‐dependent contraction of atherosclerotic mouse abdominal aortas

The objective of this study was to determine the role of cyclooxygenase (COX)‐1 or ‐2 in endothelium‐dependent contraction under atherosclerotic conditions. Atherosclerosis was induced in apoE knockout (apoE−/−) mice and those with COX‐1−/− (apoE−/−‐COX‐1−/−) by feeding with high fat and cholesterol food. Aortas (abdominal or the whole section) were isolated for functional and/or biochemical analyses. As in non‐atherosclerotic conditions, the muscarinic receptor agonist acetylcholine (ACh) evoked an endothelium‐dependent, COX‐mediated contraction following NO synthase (NOS) inhibition in abdominal aortic rings from atherosclerotic apoE−/− mice. Interestingly, COX‐1 inhibition not only abolished such a contraction in rings showing normal appearance, but also diminished that in rings with plaques. Accordingly, only a minor contraction (<30% that of apoE−/− counterparts) was evoked by ACh (following NOS inhibition) in abdominal aortic rings of atherosclerotic apoE−/−‐COX‐1−/− mice with plaques, and none was evoked in those showing normal appearance. Also, the contraction evoked by ACh in apoE−/−‐COX‐1−/− abdominal aortic rings with plaques was abolished by non‐selective COX inhibition, thromboxane‐prostanoid (TP) receptor antagonism, or endothelial denudation. Moreover, it was noted that ACh evoked a predominant production of the prostacyclin (PGI2, which mediates abdominal aortic contraction via TP receptors in mice) metabolite 6‐keto‐PGF1α, which was again sensitive to COX‐1 inhibition or COX‐1−/−. Therefore, in atherosclerotic mouse abdominal aortas, COX‐1 can still be the major isoform mediating endothelium‐dependent contraction, which probably results largely from PGI2 synthesis as in non‐atherosclerotic conditions. In contrast, COX‐2 may have only a minor role in such response limited to areas of plaques under the same pathological condition.
Suppressive effect of interleukin-4 neutralization differs for granulomas around Schistosoma mansoni eggs injected into mice compared with those around eggs laid in infected mice

The principal pathological manifestation of murine Schistosoma mansoni infection is the egg-induced granuloma. Synchronous pulmonary granulomas forming around intravenously injected schistosome eggs are widely used to study the immunopathology of schistosomiasis. A number of anticytokine antibody treatments have a remarkable effect in modulating granulomas in this model but little effect on the size of hepatic granulomas around laid eggs during experimental infection. To examine this discrepancy, we examined the effects of anticytokine antibodies on liver and lung granulomas around injected eggs and around eggs laid during infection in both locations. Anti-interleukin-4 (IL-4) treatment greatly reduced the volume of granulomas around eggs injected into the liver via the portal vein and around eggs injected into the lung via the tail vein. On the contrary, granulomas around eggs laid by worms in either the liver or the lung during the course of infection were not significantly decreased in size by anti-IL-4 treatment. Thus, site is not important for the disparate effects of anti-IL-4 in granuloma formation around injected versus laid eggs. This effect is seen in naive and sensitized animals and is most probably due to differences in the quality of injected eggs versus those laid in situ by the worms.
Machine Learning Takes on Health Care: Leonard D'Avolio's Cyft Employs Big Data to Benefit Patients and Providers

When Leonard D'Avolio (Figure 1) was working on his Ph.D. degree in biomedical informatics, he saw the power of machine learning in transforming multiple industries; health care, however, was not among them. "The reason that Amazon, Netflix, and Google have transformed their industries is because they have embedded learning throughout every aspect of what they do. If we could prove that is possible in health care too, I thought we would have the potential to have a huge impact," he says.
Vibrio cholerae-Induced Inflammation in the Neonatal Mouse Cholera Model

ABSTRACT Vibrio cholerae is the causative agent of the acute diarrheal disease of cholera. Innate immune responses to V. cholerae are not a major cause of cholera pathology, which is characterized by severe, watery diarrhea induced by the action of cholera toxin. Innate responses may, however, contribute to resolution of infection and must be required to initiate adaptive responses after natural infection and oral vaccination. Here we investigated whether a well-established infant mouse model of cholera can be used to observe an innate immune response. We also used a vaccination model in which immunized dams protect their pups from infection through breast milk antibodies to investigate innate immune responses after V. cholerae infection for pups suckled by an immune dam. At the peak of infection, we observed neutrophil recruitment accompanied by induction of KC, macrophage inflammatory protein 2 (MIP-2), NOS-2, interleukin-6 (IL-6), and IL-17a. Pups suckled by an immunized dam did not mount this response. Accessory toxins RtxA and HlyA played no discernible role in neutrophil recruitment in a wild-type background. The innate response to V. cholerae deleted for cholera toxin-encoding phage (CTXϕ) and part of rtxA was significantly reduced, suggesting a role for CTXϕ-carried genes or for RtxA in the absence of cholera toxin (CTX). Two extracellular V. cholerae DNases were not required for neutrophil recruitment, but DNase-deficient V. cholerae caused more clouds of DNA in the intestinal lumen, which appeared to be neutrophil extracellular traps (NETs), suggesting that V. cholerae DNases combat NETs. Thus, the infant mouse model has hitherto unrecognized utility for interrogating innate responses to V. cholerae infection.
The Impact of Alexithymia on Relationship Quality and Satisfaction Following Traumatic Brain Injury

Objectives:To explore relationship quality and satisfaction in couples following traumatic brain injury (TBI), obtaining the perspective of both patients with TBI and their noninjured partner; to examine the impact of alexithymia on ratings of relationship quality and satisfaction following TBI. Participants:A total of 47 patients with TBI and their noninjured partners. Measures:Twenty-item Toronto Alexithymia Scale; Index of Marital Satisfaction; and Dyadic Adjustment Scale. Results:Significant differences emerged between patients with TBI and their noninjured partners on measures of relationship quality and satisfaction, with patients reporting significantly fewer relationship problems and greater levels of overall relationship satisfaction. In contrast, the noninjured partners of patients with TBI and acquired alexithymia reported significantly lower ratings of overall relationship quality, adjustment, consensus, and cohesion. Conclusion:The presence of alexithymia in persons with TBI is an important factor affecting relationship quality and satisfaction. Future research should continue to explore the impact of emotional deficit disorders following TBI on the quality of relationships following injury and, in turn, the risk of relationship breakdown.
Voltage‐dependent magnesium block of adenosine‐triphosphate‐sensitive potassium channel in guinea‐pig ventricular cells.

1. The adenosine‐5'‐triphosphate (ATP)‐sensitive K+ channel of guinea‐pig ventricular cells was examined in the presence and absence of internal Mg2+ or Na+ using an open cell‐attached configuration of the patch‐clamp technique. 2. Millimolar concentrations of internal Mg2+ ([Mg2+]i) produced marked fluctuations in the outward current, and the amplitude of the open‐channel current was reduced with increasing [Mg2+]i. Millimolar Na+ applied internally also decreased the mean amplitude of the outward current, but the increase in current noise was not obvious. These effects became larger when the membrane potential was shifted to be more positive from the K+ equilibrium potential (EK). At potentials negative to EK the inward current was affected by neither internal Mg2+ nor Na+. 3. The external application of Na+, Mg2+ or Ca2+, however, failed to affect the single‐channel current. 4. After removal of both internal Mg2+ and Na+, the mean open‐channel current‐voltage relationship became virtually linear. Referring to these unblocked values, relative amplitudes were determined at different levels of [Mg2+]i or [Na+]i. The dose‐response relations gave a Hill coefficient of approximately 1 for Mg2+ block and approximately 2 for Na+ block. The half‐maximum concentrations (Kh) for both Mg2+ and Na+ block were shifted to lower values with increasing positive potentials. 5. The power‐density spectrum of the open‐channel current noise induced by internal Mg2+ showed a Lorentzian function with a corner frequency above 1 kHz, suggesting that the current noise is due to rapid fluctuations of open‐channel current between blocked and unblocked states. The corner frequencies gave Mg2+ block and unblock rate constants which were of the order of 10(7) M‐1 s‐1 and 10(4) s‐1, respectively. 6. With increasing external K+ concentration ([K+]o) from 0 to 140 mM the current fluctuations became less prominent, and Kh for Mg2+ block was shifted to higher values. Raising [K+]o enhanced the unblock rate derived from the noise analysis while the block rate was not significantly altered. 7. The above findings could be explained by assuming a binding site for one Mg2+ or two Na+ located 30‐35% of the electrical drop across the membrane from the inner mouth of the channel, thereby resulting in the ionic block of K+ passage. An apparent inward rectification observed in the single‐channel current‐voltage relation is attributable to the blockade of the channel by intracellular Mg2+ and/or Na+.
Dysfunction of protein kinase FA/GSK-3 alpha in lymphocytes of patients with schizophrenic disorder.

As compared to normal people, the lymphocytes of patients with schizophrenia were found to have an impairment of ATP.Mg-dependent protein phosphatase activation. More importantly, the impaired protein phosphatase activation in the lymphocytes of schizophrenic patients could be consistently and completely restored to normal by exogenous pure protein kinase FA/glycogen synthase kinase-3 alpha (kinase FA/GSK-3 alpha) (the activating factor of ATP.Mg-dependent protein phosphatase), indicating that the molecular mechanism for the impaired protein phosphatase activation in schizophrenic patients may be due to a functional loss of kinase FA/GSK-3 alpha. Immunoblotting and kinase activity analysis in an anti-kinase FA/GSK-3 alpha immunoprecipitate further demonstrate that both cellular activities and protein levels of kinase FA/GSK-3 alpha in the lymphocytes of schizophrenic patients were greatly impared as compared to normal controls. Statistical analysis revealed that the lymphocytes isolated from 37 normal people contain kinase FA/GSK-3 alpha activity in the high levels of 14.8 +/- 2.4 units/mg of cell protein, whereas the lymphocytes of 48 patients with schizophrenic disorder contain kinase FA/GSK-3 alpha activity in the low levels of 2.8 +/- 1.6 units/mg, indicating that the different levels of kinase FA/GSK-3 alpha activity between schizophrenic patients and normal people are statistically significant. Taken together, the results provide initial evidence that patients with schizophrenic disorder may have a common impairment in the protein levels and cellular activities of kinase FA/GSK-3 alpha, a multisubstrate protein kinase and a multisubstrate protein phosphatase activator in their lymphocytes.
Improved neuronal transgene expression from an AAV‐2 vector with a hybrid CMV enhancer/PDGF‐β promoter

Adeno‐associated virus type 2 (AAV‐2) vectors are highly promising tools for gene therapy of neurological disorders. After accommodating a cellular promoter, AAV‐2 vectors are able to drive sustained expression of transgene in the brain. This study aimed to develop AAV‐2 vectors that also facilitate a high level of neuronal expression by enhancing the strength of a neuron‐specific promoter, the human platelet‐derived growth factor β‐chain (PDGF) promoter.
Immigration and the ephemerality of a natural population bottleneck: evidence from molecular markers

Population bottlenecks are often invoked to explain low levels of genetic variation in natural populations, yet few studies have documented the direct genetic consequences of known bottlenecks in the wild. Empirical studies of natural population bottlenecks are therefore needed, because key assumptions of theoretical and laboratory studies of bottlenecks may not hold in the wild. Here we present microsatellite data from a severe bottleneck (95% mortality) in an insular population of song sparrows (Melospiza melodia). The major findings of our study are as follows: (i) The bottleneck reduced heterozygosity and allelic diversity nearly to neutral expectations, despite non–random survival of birds with respect to inbreeding and wing length. (ii) All measures of genetic diversity regained pre–bottleneck levels within two to three years of the crash. This rapid recovery was due to low levels of immigration. (iii) The rapid recovery occurred despite a coincident, strong increase in average inbreeding. These results show that immigration at levels that are hard to measure in most field studies can lead to qualitatively very different genetic outcomes from those expected from mutations only. We suggest that future theoretical and empirical work on bottlenecks and metapopulations should address the impact of immigration.
Special Interest Groups Versus Voters and the Political Economics of Attention

Asymmetric information between voters and legislative representatives poses a major challenge to the functioning of representative democracy. We examine whether representatives are more likely to serve long-term campaign donors instead of constituents during times of low media attention to politics. Combining data on campaign finance donations made by individuals and special interest groups with information on their preferences for particular bills, we construct novel measures of electoral and organized interests pressure that representatives face with regard to specific legislative votes. In our analysis based on 490 roll calls between 2005 and 2014 in the US House of Representatives, we find strong evidence that representatives are more likely to vote with special interests and against constituency interests when the two are in conflict. Importantly, the latter effect is significantly larger when there is less attention on politics. Thereby, we draw on exogenous newsworthy shock events that crowd out news on the legislative process, but are themselves not related to it. The opportunistic behavior seems not to be mediated by short-term scheduling of sensitive votes right after distracting events.
And yet it matters: referendum campaigns and vote decision in Eastern Europe

ABSTRACT Referendums campaigns are important and earlier research closely analysed their general functioning, effects on turnout, and the importance of media and information for voting behaviour. However, the role of referendum campaigns as such (with all its components) in shaping voting behaviour was widely neglected. This article seeks to partially fill this gap in the literature and argues that referendum campaign is an important predictor of the voting decision as long as people perceive it as informative and follow it. We investigate this effect in the context of three referendums organized in 2015–2016 in Bulgaria, Poland and Slovakia. The results indicate that these two variables explain the decision of citizens to support referendums across different settings. Their effects are consistent and significantly stronger than alternative explanations employed in the literature such as the limited effect of campaigns, second-order elections, partisan cues or amount of information received.
PROTON GRADIENT REGULATION5 Is Essential for Proper Acclimation of Arabidopsis Photosystem I to Naturally and Artificially Fluctuating Light Conditions[W]

This work demonstrates that photosystem I, not photosystem II, is susceptible to photodamage under natural fluctuations in light intensity in Arabidopsis thaliana. PGR5 is an indispensable protein for protection of photosystem I, particularly in young plants. PGR5-mediated protection takes place via regulation of linear electron transfer, rather than via cyclic electron transfer. In nature, plants are challenged by constantly changing light conditions. To reveal the molecular mechanisms behind acclimation to sometimes drastic and frequent changes in light intensity, we grew Arabidopsis thaliana under fluctuating light conditions, in which the low light periods were repeatedly interrupted with high light peaks. Such conditions had only marginal effect on photosystem II but induced damage to photosystem I (PSI), the damage being most severe during the early developmental stages. We showed that PROTON GRADIENT REGULATION5 (PGR5)–dependent regulation of electron transfer and proton motive force is crucial for protection of PSI against photodamage, which occurred particularly during the high light phases of fluctuating light cycles. Contrary to PGR5, the NAD(P)H dehydrogenase complex, which mediates cyclic electron flow around PSI, did not contribute to acclimation of the photosynthetic apparatus, particularly PSI, to rapidly changing light intensities. Likewise, the Arabidopsis pgr5 mutant exhibited a significantly higher mortality rate compared with the wild type under outdoor field conditions. This shows not only that regulation of PSI under natural growth conditions is crucial but also the importance of PGR5 in PSI protection.
Autonomous flight control for RC helicopter using camera image

In recent years, there are a lot of research on the subject of autonomous flight control using a small radio control helicopter. An autonomous flight control system for a RC helicopter needs to get three dimensional position and attitude, methods using a GPS/IMU and various sensors, and methods using input images from camera have been investigated. However, usable environments are limited according to selection of sensors. On the other hands, methods using input camera images need to setup many artificial markers otherwise an estimation error will accumulate. In this research, we propose a RC helicopter position and attitude estimation method for flight control in a wide area which does not require sensors and many artificial markers by detecting natural feature points from camera images. In this paper, we report about the measurement of three dimensional position and attitude of the RC helicopter and the feature point extraction.
Dynamically evoked, discrete-threshold electromyography in the extreme lateral interbody fusion approach.

OBJECT because the psoas muscle, which contains nerves of the lumbar plexus, is traversed during the extreme lateral interbody fusion (XLIF) approach, appropriate nerve monitoring is needed to avoid nerve injury during surgery and prevent approach-related neural deficit. This study was performed to assess the effectiveness of dynamically evoked electromyography (EMG) to detect and prevent neural injury during the XLIF approach. METHODS one hundred two patients undergoing XLIF at L3-4 and/or L4-5 were enrolled in a prospective, multicenter, nonrandomized clinical study. The EMG threshold values for each of the 3 successive dilators were recorded at the surface of the psoas muscle, mid-psoas, and on the spine. At each location, the dilators were rotated 360°, taking recordings immediately posterior, superior, anterior, and inferior. For each dilator, the authors noted the rotational position (the angle in degrees) at which the lowest threshold was found. Findings of pre- and postoperative neurological examinations were also recorded. RESULTS nerves were identified within proximity of the dilators (alert-level EMG feedback) in 55.7% of all cases during the XLIF approach. Although nerves were more commonly identified in the posterior margin (63%), there was significant variability in the location of nerves identified. Despite the fact that the posterior half of the disc space was targeted in 90% of cases, no significant long-lasting neural deficits were identified in any case; 27.5% experienced new iliopsoas/hip flexion weakness and 17.6% experienced new postoperative upper medial thigh sensory loss. Transient motor deficits were identified in 3 patients (2.9%), and all had resolved by the 6-month follow-up visit. CONCLUSIONS the ability to identify and report a discrete, real-time EMG threshold during the transpsoas approach helps to avoid nerve injury and is required for the safe performance of the XLIF procedure. Additionally, nerve location is variable, thus reinforcing the need for real-time directional and proximity information.
GPU backed Data Mining on Android Devices

Choosing an appropriate programming paradigm for high-performance computing on low-power devices can be useful to speed up calculations. Many Android devices have an integrated GPU and - although not officially supported - the OpenCL framework can be used on Android devices for addressing these GPUs. OpenCL supports thread and data parallelism. Applications that use the GPU must account for the fact that they can be suspended by the user or the Android operating system at any moment. We have created a wrapper library that allows to use OpenCL on Android devices. Already written OpenCL programs can be executed with almost no modification. We have used this library to compare the performance of the DBSCAN and Kmeans algorithms on an integrated GPU of an Arm-v7 tablet with other single and multithreaded implementations on the same device. We have investigated which programming paradigm and language allows the best tradeoff between execution speed and energy consumption. Using the GPU for HPC on Android devices can help to carry out computationally intensive machine learning or data mining tasks in remote areas, under harsh environmental conditions and in areas where energy supply is an issue.
Psychosocial Factors Related to Posttraumatic Growth in Breast Cancer Survivors: A Review

In this article, we reviewed quantitative studies regarding psychosocial factors associated with posttraumatic growth (PTG) in patients with breast cancer to elucidate our understanding of a model of PTG process. PsycInfo, Embase, Medline, Web of Knowledge were used for the search. Only quantitative, English written studies that used the Posttraumatic Growth Inventory (PTGI) measure administered to breast cancer patients were included. The initial search yielded 90 publications. Of those, 22 studies satisfied inclusion criteria and formed the basis of the review. Personality traits (e.g., optimism and openness), cognitive processing of cancer (e.g., deliberate rumination), perceived threat of the disease, coping strategies (e.g., problem-focused), and social support were identified to be related to PTG in women with breast cancer. Demographic characteristics (e.g., age at cancer diagnosis) were also found to play a key role in PTG. The findings of this review provided support to Tedeschi and Calhoun’s functional–descriptive model of PTG process. Further directions for research and clinical implications are provided.