Miracle-dz/UniFAR-FTUs huggingface dataset:
Histone Deacetylase 6 (HDAC6) Deacetylates Survivin for Its Nuclear Export in Breast Cancer*

Background: Survivin is an oncogenic protein that is acetylated by CBP, which restricts its location to the nuclear compartment and blocks its anti-apoptotic effect. Results: HDAC6 deacetylates survivin to promote its nuclear exit in estrogen receptor-positive breast cancer cells. Conclusion: Cross-talk between estrogen, CBP, and HDAC6 regulate the amount of nuclear acetylated survivin. Significance: Understanding how estrogen regulates survivin nuclear export may influence breast cancer treatment. Survivin is an oncogenic protein that is highly expressed in breast cancer and has a dual function that is dependent on its subcellular localization. In the cytosol, survivin blocks programmed cell death by inactivating caspase proteins; however, in the nucleus it facilitates cell division by regulating chromosomal movement and cytokinesis. In prior work, we showed that survivin is acetylated by CREB-binding protein (CBP), which restricts its localization to the nuclear compartment and thereby inhibits its anti-apoptotic function. Here, we identify histone deacetylase 6 (HDAC6) as responsible for abrogating CBP-mediated survivin acetylation in the estrogen receptor (ER)-positive breast cancer cell line, MCF-7. HDAC6 directly binds survivin, an interaction that is enhanced by CBP. In quiescent breast cancer cells in culture and in malignant tissue sections from ER+ breast tumors, HDAC6 localizes to a perinuclear region of the cell, undergoing transport to the nucleus following CBP activation where it then deacetylates survivin. Genetically modified mouse embryonic fibroblasts that lack mhdac6 localize survivin predominantly to the nuclear compartment, whereas wild-type mouse embryonic fibroblasts localize survivin to distinct cytoplasmic structures. Together, these data imply that HDAC6 deacetylates survivin to regulate its nuclear export, a feature that may provide a novel target for patients with ER+ breast cancer.
Evaluation of Acha (Digitaria exilis) Grain Fermented with Lactobacillus Species as a Probiotic Food

Aims: This study assess the effect of the fermented Acha samples in-vivo using apparently healthy and infected laboratory animals. Study Design: Acha was fermented in two forms (Local fermentation and controlled fermentation). Place and Duration of Study: Sample: Department of Medicine (Medical Unit IV) and Department of Radiology, Services Institute of Medical Sciences (SIMS), Services Hospital Lahore, between June 2009 and July 2010. Methodology: Acha was weighed into a fermenting container of 100 g and water of 1 litre was added to submerge it for 72 hours in the ratio 1:3. Microbial, proximate and mineral analysis was carried on all the samples. For 21 days, all fermented samples were used to feed rats infected with Escherichia coli and Shigella dysenteriae except for the control for in vivo study and evaluated for their probiotic potential. Also, hematological study and histopathology analysis were carried out on the small and large intestine of the Albino rats that was fed with the fermented samples. The various fermented samples were freeze dried to retain the organisms used for the fermentation. Results: Haematological study (PCV, WBC, RBC, Platelets, haemoglobin and differential leucocytes) and histopathology analysis (small intestine and large intestine) of rats from all experimental groups showed that Acha fermented with Lactobacillus acidophilus was able to rebuild shrinked and ruptured cells on the mucosal lining of the walls of the intestines. Conclusion: Acha fermented with Lactobacillus acidophilus was observed to have the best results on the weight of rats, white blood cell count, red blood cell count and probiotic effect on the intestine of the rats fed with it.
Indoor Localization with Aircraft Signals

The standard method for outdoor localization is GPS, because it is globally available, relatively accurate and receivers are inexpensive. However, GPS does not work well indoors due to low signal strength. We explore a new localization approach, which uses the same principle as GPS localization, but employs signals transmitted by aircraft. Compared to GPS, aircraft signals are strong and can be received indoors. Our prototype implementation achieves a user localization accuracy of approximately 25 meters.
ETANOL DE PRIMEIRA OU DE SEGUNDA GERAÇÃO? UMA COMPARAÇÃO ENTRE OS CICLOS PRODUTIVOS

The purpose of this research is to investigate and compare both First Generation Ethanol (FGE) and Second Generation Ethanol (SGE) life-cycles, under economical, technological, environmental and social aspects in Brazil, by means of literature review and expert interview. FSE demonstrated both economic and environmental advantages in contrast with fossil fuels; however, in spite of developments in mechanization aimed at the agriculture stage and the recent growth of schooling levels, FSE still stands far from non-agricultural industries. SGE indicates significant environmental and social advantages, correlated to FSE, due to its decreased use of natural resources and energy, its feedstock being FSE solid residue, and the increase in technology with better qualified labor. The FSE-SGE combinedcycle is a viable proposition to overcome these challenges on behalf of the sugarcane plants. Key-words: Ethanol, First Generation, Second Generation, Brazil.
Incorporating transportation time in multi-agent production network scheduling

The scheduling of factories that work in production network is a new type of scheduling problem that all of the developed single factory techniques are inappropriate for it. The aim of this paper is to propose the scheduling algorithm for such environment in which several factories disperse geographically in different places with parallel machines and each factory as a production agent may have a different objective function. We assume there are two types of production agent, i.e. some factories are interested in the sum of completion times and the remaining factories are interested in the makespan. In such system, a schedule should give enough flexibility to a local scheduler. This can be attained by transporting the jobs among factories from the overloaded machine to the machine which has fewer workloads. By incorporating the transportation assumption in problem definition, we first present a mathematical modelling for the new scheduling problem. We then used CPLEX solver to obtain Pareto solutions by applying ∈-constraint approach. Furthermore, in addition to a genetic algorithm (GA), we proposed a new evolutionary metaheuristic namely imperialist competitive algorithm (ICA) that armed with a new encoding scheme. Finally, the outputs obtained from mathematical algorithm, ICA and GA are reported.
OPTIMAL GROWTH CONDITIONS AND ANTIOXIDATIVE ACTIVITIES OF CYLINDROTHECA CLOSTERIUM (BACILLARIOPHYCEAE) 1

We isolated the unialgal strain of Cylindotheca closterium (Ehrenb.) Reimann et J. C. Lewin and produced an axenic strain using an antibiotic cocktail of enriched f/2 artificial seawater medium. The optimal growth conditions were estimated under 27 different combinations of temperature, salinity, and nutrients, and mass culture was performed based on the best specific growth conditions. Its antioxidant activities were determined from the extracts of methanol, water, and enzymes (proteases and carbohydrases). The maximum specific growth rate (μmax) varied from 0.63 to 0.97 · d−1. The maximum cell density was 7.20 × 104 cells · mL−1, while the μmax was 0.82 · d−1 in culture conditions of 20°C, 30 psu (practical salinity unit), and “F” nutrient concentrations on day 10 of the culture period. The scavenging rates for 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) free radical were 72.5% and 69.4% from Viscozyme and methanol extracts, respectively. The enzymatic extracts of C. closterium prepared by the hydrolyses of Amyloglucosidase (AMG) and Viscozyme showed 45.8% and 45.5% nitric‐oxide‐scavenging rates, slightly lower than the activity of alpha‐tocopherol (α‐tocopherol) but similar to butylated hydroxytoluene (BHT). The extract from methanol and water showed 44.8% and 44.4% scavenging rates, statistically similar with BHT. The metal‐chelating activities of the Kojizyme, Alcalase, methanol, Viscozyme, and Neutrase extracts were 67.1, 53.9, 53.2, 52.1, and 50.2 %, respectively, five to six times higher than the commercial antioxidants. The AMG, Viscozyme, and Neutrase extracts showed a remarkable linoleic acid peroxidation inhibition, which was higher than BHT and statistically similar with α‐tocopherol.
Ecological Limits on Clade Diversification in Higher Taxa

Species richness varies dramatically among groups of organisms, yet the causes of this variation remain poorly understood. Variation in species‐level diversification rates may partially explain differential species richness among clades, but older clades should also be more diverse, because they will have had more time to accumulate species. Surprisingly, studies that have investigated this question have reached dramatically different conclusions: several claim to find no such age‐diversity relationship, whereas a recent and more inclusive study reported that clade age and not diversification rate explains the variation in species richness among animal taxa. Here I address the relationship between clade age and species richness using a model‐based approach that controls for variation in diversification rates among clades. I find that species richness is effectively independent of clade age in four of five data sets. Even extreme among‐clade variation in diversification rates cannot account for the absence of a positive age‐diversity relationship in angiosperms, birds, and teleost fishes. I consider two alternative explanations for these results and find that a clade volatility model positing correlated speciation‐extinction dynamics does not underlie these patterns. Rather, ecological limits on clade growth, such as geographic area, appear to mediate temporal declines in diversification within higher taxa.
Diagnosis of and Screening for Cytomegalovirus Infection in Pregnant Women

ABSTRACT No single diagnostic test for cytomegalovirus (CMV) infection is currently available for pregnant women at all stages of gestation. Improved accuracy in estimating the timing of primary infections can be used to identify women at higher risk of giving birth to congenitally infected infants. A diagnostic algorithm utilizing immunoglobulin G (IgG), IgM, and IgG avidity was used to prospectively screen serum from 600 pregnant women enrolled from two groups: ≤20 weeks gestation (n = 396) or >20 weeks gestation (n = 204). PCR testing of urine and/or blood was performed on all seropositive women (n = 341). The majority (56.8%) of women were CMV IgG seropositive, with 5.5% being also CMV IgM positive. In the IgM-positive women, 1.2% had a low-avidity IgG, indicating a primary CMV infection and a high risk of intrauterine transmission. Two infants with asymptomatic CMV infection were born of mothers who had seroconverted in the second trimester of pregnancy. Baseline, age-stratified CMV serostatus was established from 1,018 blood donors. Baseline seropositivity from a blood donor population increased with age from 34.9% seroprevalence at less than 20 years of age to 72% seroprevalence at 50 years of age. Women at high risk of intrauterine transmission of CMV were identified at all stages of gestation. Women infected with CMV during late gestation may be more likely to transmit the virus, so failure to detect seroconversions in late gestation may result in failure to detect infected neonates.
Rule-based algorithm for the classification of sitting postures in the sagittal plane from the Cardiff Body Match measurement system

Abstract The purpose of this paper is to present the design and implementation of a novel rule-based algorithm for the classification of sitting postures in the sagittal plane. The research focused on individuals with severe musculoskeletal problems and, thus, specific requirements for posture and pressure management. Clients’ body shapes were captured using the Cardiff Body Match system developed by the Rehabilitation Engineering Unit, Cardiff and Vale University Health Board. The algorithm consists of four main steps: the first step is the symmetry line detection, the second step involves the mathematical analysis of the curvature of the backrest profile, the third step is the sitting posture classification and the fourth step is the extraction of the geometric parameters from the curve. The results show the classification system was successful in identifying four types of curves characterizing sitting postures using local derivatives as curve descriptors with an overall accuracy of 93.9%.
Predicting Stock Prices Using LSTM

: The art of forecasting the stock prices has been a difficult task for many of the researchers and analysts. In fact, investors are highly interested in the research area of stock price prediction. For a good and successful investment, many investors are keen in knowing the future situation of the stock market. Good and effective prediction systems for stock market help traders, investors, and analyst by providing supportive information like the future direction of the stock market. In this work, we present a recurrent neural network (RNN) and
E6* oncoprotein expression of human papillomavirus type‐16 determines different ultraviolet sensitivity related to glutathione and glutathione peroxidase antioxidant defence

Abstract: Clinical observations of non‐melanoma skin cancer in immunocompromised patients, such as organ transplant recipients, suggest co‐operative effects of human papillomavirus (HPV) and ultraviolet (UV) radiation. The aim of the present study is to evaluate UV sensitivity and DNA damage formation according to antioxidant status in HPV16‐infected keratinocytes. We used SKv cell lines, infected with HPV16 and well characterized for their proliferative and tumorigenic capacities. We showed that SKv cell lines presented various E6* (a truncated form of E6) RNA levels. We demonstrated that the higher oncoprotein RNA expression level was associated with a higher resistance to solar‐simulated radiation, more specifically to UVB radiation and to hydrogen peroxide. Moreover, this high resistance was associated with a low oxidative DNA damage formation after UV radiation and was related to high glutathione content and glutathione peroxidase activities. Therefore, the results of our study suggest that E6* levels could modulate the glutathione/glutathione peroxidase pathway providing a mechanism to protect HPV‐infected keratinocytes against an environmental oxidative stress, such as UV radiation.
The Genetic Basis of Singlet OxygenInduced Stress Responses of Arabidopsis thaliana

Plants under oxidative stress suffer from damages that have been interpreted as unavoidable consequences of injuries inflicted upon plants by toxic levels of reactive oxygen species (ROS). However, this paradigm needs to be modified. Inactivation of a single gene, EXECUTER1, is sufficient to abrogate stress responses of Arabidopsis thaliana caused by the release of singlet oxygen: External conditions under which these stress responses are observed and the amounts of ROS that accumulate in plants exposed to these environmental conditions do not directly cause damages. Instead, seedling lethality and growth inhibition of mature plants result from genetic programs that are activated after the release of singlet oxygen has been perceived by the plant.
Microsomal Opiate Receptors: Characterization of Smooth Microsomal and Synaptic Membrane Opiate Receptors

Abstract: In continuing studies on smooth microsomal and synaptic membranes from rat forebrain, we compared the binding properties of opiate receptors in these two discrete subcellular populations. Receptors in both preparations were saturable and stereospecific. Scatchard and Hill plots of [3H]naloxone binding to microsomes and synaptic membranes were similar to plots for crude membranes. Both synaptic membranes and smooth microsomes contained similar enrichments of low‐ and high‐affinity [3H]naloxone binding sites. No change in the affinity of the receptors was observed. When [3H]D‐ala2‐D‐leu5‐enkephalin was used as ligand, microsomes possessed 60% fewer high‐affinity sites than did synaptic membranes, and a large number of low‐affinity sites. In competition binding experiments microsomal opiate receptors lacked the sensitivity to (guanyl‐5′‐yl)imidodiphosphate [Gpp(NH)p] shown by synaptic and crude membrane preparations. In this respect microsomal opiate receptors resembled membranes that were experimentally guanosine triphosphate (GTP)‐uncoupled with N‐ethylmaleimide (NEM). Agonist binding to microsomal and synaptic membrane opiate receptors was decreased by 100 mM NaCl. Like NEM‐treated crude membranes, microsomal receptors were capable of differentiating agonist and antagonists in the presence of 100 mM NaCl. MnCl2 (50–100 μM) reversed the effects of 100 mM NaCl and 50 μM GTP on binding of the μ‐specific agonist [3H]dihydromorphine in both membrane populations. Since microsomal receptors are unable to distinguish agonists from antagonists in the presence of Gpp(NH)p, they are a convenient source of guanine nucleotide‐uncoupled opiate receptors.
Long-Term Stability of Parental Representations in Depressed Outpatients Utilizing the Parental Bonding Instrument

This study addressed two questions: (a) What are the 30-month, 60-month, and 90-month test-retest reliabilities of the Parental Bonding Instrument (PBI); and (b) are recollections of parental bonding stable across changes in the level of depressed mood? Participants included 97 outpatients with primary early-onset dysthymic disorder and 45 outpatients with episodic major depressive disorder. Follow-ups were conducted at 30, 60, and 90 months after entry into the study. The PBI was scored using both the original two-factor model and a three-factor solution. As indexed by intraclass correlations, stability for the original two-factor model ranged from . 64 to . 88, with a median of . 77. The intraclass correlations for the three-factor model ranged from . 54 to . 87, with a median of . 73. The intraclass correlations were generally higher between adjacent assessments, but the decrease over increasingly longer intervals was quite small. Overall, the results indicated that the PBI is highly stable for intervals as long as 90 months. In addition, reports of parental bonding were relatively stable despite significant changes in the level of depressed mood. Thus, the present study provides evidence for the long-term stability of parental representations, as measured by the PBI, in depressed outpatients.
Hábitos de los adolescentes sobre el uso de las redes sociales: caso de estudio en secundarias públicas

Actualmente todos los adolescentes desean contar con tecnologia de punta que les permita estar comunicados a traves del Internet, por tal razon en este articulo se realizo una investigacion sobre el uso que los alumnos de 3er. Grado de las secundarias publicas en la zona de Ocotlan, Jalisco le dan a las Redes Sociales. Para determinar cuales son las que mas utilizan, para que las usan, que informacion comparten, si estan monitoreados por sus padres, tambien se detecta si realizan actividades de Ciberbullying; o si las emplean como material didactico para estar en contacto con sus profesores. Posteriormente se realizan recomendaciones que tanto padres como profesores deben tomar en cuenta para que el impacto de las Redes Sociales en los estudiantes sea positivo y no negativo; para evitar que adquieran adiccion a las Redes Sociales o tengan algun peligro.
Hypoxia induces lineage modulation of Ewing’s sarcoma tumor cells into EWS-FLI-1 + vascular pericytes

Background: Vasculogenesis and angiogenesis are required for expansion of the Ewing’s sarcoma vasculature. Our previous studies demonstrated that pericytes and DLL4 Notch signaling pathway are critical to the formation of new tumor vessels, but how tumor microenvironment regulates tumor vasculature is not well understood. Methods: Using unique EWS-FLI-1 fusion protein as tumor hallmark to determine tumor cell phenotype in pericytes. Investigate that hypoxia induced Ewing’s sarcoma (ES) tumor cells express stem cell characteristics and transdifferentiated into pericytes. Identify pericyte property in ES tumor cells by transfection of special Desmin promoter-driven GFP vector. Results: We discovered that a subset of tumor vascular pericytes expressed EWS-FLI-1 in Ewing’s sarcoma patient tumor samples and xenograft mouse tumor vessels suggesting that these pericytes originated from Ewing’s sarcoma tumor cells. These EWS-FLI-1 + pericytes were in hypoxic areas. Culturing TC71 and A4573 Ewing’s sarcoma cells under hypoxic condition induced sphere formation, and up-regulation of stem cell and pericyte markers. This hypoxia-induced lineage modulation was in the CD133+ tumor cells, enhanced by DLL4 and inhibited by a ? -secretase inhibitor. To confirm that Ewing’s tumor cells transdifferentiated into pericytes, TC71 and A4573 cells were transfected with a Desmin promoter-driven GFP vector. Culturing these transfected cells under hypoxic condition resulted in GFP expression confirming differentiation into a pericyte lineage. Injecting transfected cells into mice resulted in a subset of tumor vascular pericytes that expressed GFP. Conclusion: This is the first to demonstrate that hypoxic tumor microenvironment triggers Ewing’s sarcoma tumor cells transdifferentiated into pericytes that contribute to tumor vessel formation. These novel findings suggest that an additional therapeutic approach may inhibit tumor vascular expansion, tumor growth and metastasis.
CAPILLARY-BASED MICROREACTOR FOR SCREENING PEPTIDE CATALYSTS IN THE ALDOL REACTION

In recent years, the concept of miniaturization has been applied to many areas of chemistry, one of which is the synthesis arena with the development of new microreactor technology. Compared with conventional scale vessels, microreactors are advantageous in many aspects, such as faster mixing, better heat transfer, minimal reagent use and great safety. However, the technology of microreactors is still immature and most organic reactions are simply carried out based on the existing concept and setup of 'lab-on-a-chip'. The analysis-oriented chips are always incompatible with demands of organic synthesis and so the development of synthesis-oriented microreactors is of great need. Our laboratory developed a novel capillary-based reactor system that was specific for high-throughput synthesis and screening. The computer-controlled reactor system integrated standard HPLC apparatus (autosampler, pump), fused-silica capillaries and GC in which separate zones of reactants and catalysts can be combined and loaded serially into a single reactor capillary, reacted in parallel and ejected serially for online GC analysis. One of the applications of our microreactor was to study peptide-catalyzed aldol reaction. We chose a model aldol reaction with benzaldehyde and acetone substrates and known catalyst L-proline. Chiral GC separation conditions were optimized for determination of chiral aldol products. The optimum reaction conditions were 10 mol% L-proline catalyst, DMSO and acetone 1:1 (v/v), room temperature and 4 hr reaction time. A little amount of acetic acid was added to increase L-proline solubility in organic solvents. Several peptides were preliminarily screened in the microreactor. Unfortunately, all of them showed poor activities. The next step is to keep screening active peptide catalysts by our microreactor. Besides, novel solvents will be studied to further increase product yield and selectivity. The microreactor will also be optimized to increase its throughput and efficiency. The optimization process will be based on the combination of mathematical calculation (Mathcad software) and experiments. Moreover, the design of the microreactor will be improved in some units to make the system capable of accommodating more types of reactions such as multi-step reactions, gas-phase reactions or gas/liquid multiphase reactions.