Miracle-dz/UniFAR-FTUs huggingface dataset:
A Novel Validated Stability Indicating Analytical Method for Simultaneous Quantification of Metformin Hydrochloride and Empagliflozin in Bulk and Marketed Formulation by HPTLC using Box-Wilson Experimental Design Approach

Background: A novel stability indicating analytical method was developed and validated by High Performance Thin Layer Chromatography (HPTLC) using Design of experiment approach. The proposed method is useful for quantification of Metformin hydrochloride and Empagliflozin in bulk and its dosage forms simultaneously. Design of experiment approach was applied for optimization of chromatographic conditions. Materials and Methods: For optimization process independent variables were used as Isopropyl alcohol proportion in mobile phase, saturation time of chamber and distance travelled by mobile phase. Experiments were carried out on silica gel pre-coated plate using mobile phase as 2 % Ammonium acetate: Isopropyl alcohol: Triethylamine (4:6:0.1 v/v/v). Direct evaluation of chromatograms were done by TLC scanner with reflectance/absorbance mode set at 242 nm. Method was validated as per ICH Q2 (R1) requirements. Results: Correlation coefficients for calibration curves were found to be 0.985 and 0.988, the calibration curve is in concentration range of 5000-30000 ng band-1 and 125–750 ng band-1 for Metformin hydrochloride and Empagliflozin respectively. The method showed % recovery between 99.05 to 102.54 % for Metformin hydrochloride and 99.20 to 101.50 % for Empagliflozin. The method has a prospective to determine Metformin hydrochloride and Empagliflozin simultaneously. The Metformin hydrochloride and Empagliflozin were subjected to forced degradation studies like hydrolysis, oxidation, thermolysis and photodegradation. Conclusion: Proposed method has capacity to separate the Metformin hydrochloride and Empagliflozin in its degradation products. Hence one can apply this method effectively for routine analysis and during stability study as per regulatory requirements.
AGAMOTTO: How Persistent is your Persistent Memory Application?

Persistent Memory (PM) can be used by applications to directly and quickly persist any data structure, without the overhead of a ﬁle system. However, writing PM applications that are simultaneously correct and efﬁcient is challenging. As a result, PM applications contain correctness and performance bugs. Prior work on testing PM systems has low bug coverage as it relies primarily on extensive test cases and developer annotations. In this paper we aim to build a system for more thoroughly testing PM applications. We inform our design using a detailed study of 63 bugs from popular PM projects. We identify two application-independent patterns of PM misuse which account for the majority of bugs in our study and can be detected automatically. The remaining application-speciﬁc bugs can be detected using compact custom oracles provided by developers. We then present A GAMOTTO , a generic and extensible system for discovering misuse of persistent memory in PM applications. Unlike existing tools that rely on extensive test cases or annotations, A GAMOTTO symbolically executes PM systems to discover bugs. A GAMOTTO introduces a new symbolic memory model that is able to represent whether or not PM state has been made persistent. A GAMOTTO uses a state space exploration algorithm, which drives symbolic execution towards program locations that are susceptible to persistency bugs. A GAMOTTO has so far identiﬁed 84 new bugs in 5 different PM applications and frameworks while incurring no false positives.
Comparative Assessment of the Microbiological and Physicochemical Quality of a Laboratory Brewed ‘Burukutu’ and Commercialized Products Sold in Some Markets in Port Harcourt Nigeria

Burukutu is an indigenous, brown coloured, cloudy, alcoholic beverage traditionally brewed using mainly sorghum grains. This study was aimed at the comparison of microbial quality, physicochemical properties, proximate composition and minerals content of laboratory brewed burukutu (LBB) and commercial products sold in some markets in Port Harcourt. Sixteen (16) samples of the commercialized product were evaluated from Elele (EM), Rumuokoro (RM) and Choba (CM) markets, Port Harcourt, Rivers State using Standard Methods while LBB was produced in our laboratory using Standard Procedures. Our result indicated an increase in aerobic mesophiles, total coliforms, faecal coliforms, fungi and lactic acid bacteria (LAB) counts during the early stages of the brewing process of laboratory produced burukutu but only LAB was detected in the final product. The overall microbial count of burukutu from the three markets revealed that CM>EM>RM although all the samples had higher microbial count compared with the LBB. Staphylococcus aureus, Escherichia coli, Lactobacillus sp. , Lactobacillus sp. , Aspergillus sp. and Saccharomyces cerevisiae were present during the brewing stages of burukutu. Enterobacter aerogenes and Mucor sp. were detected in the commercial samples purchased from the three markets. Lactobacillus fermentum and L. brevis had the highest and least frequency of occurrence in all the samples, respectively. The pH, titratable acidity, alcohol content and soluble solids of the fermenting LBB were within the range of 3.03. 4, 0.6-0.97%, 3.0-4.7% and 0.98-0.99%, whereas in the commercialized samples, the equivalent values were 3.0-3.1, 0.00-0.10%, 2.1-4.0% and 0.650. 82%, respectively. Moisture, crude fat, ash, crude protein and carbohydrate content of the commercialized samples were within the range of 29.1-31.2%, 10.8-14.2%, 3.8-4.85%, 2.9-5.7%, and 44.7-51.7% compared to the range of values obtained in the LBB (52.1%, 15.7%, 9.8%, 7.6% and 15.4%), respectively. The following minerals elements K, Ca, Na, Zn and Mg present in the LBB were higher than the values obtained in the commercialized alcoholic beverage. The results from this study, suggest that the quality of LBB is better than the commercialized products. Therefore, it is recommended that in order to improve the overall quality of commercially brewed burukutu for the benefit of everyone irrespective of social status, good manufacturing practices (GMP) and good hygienic practices (GHP) should be strictly adhered to by local producers and vendors.
Aggregated Low-Density Lipoprotein Induces LRP1 Stabilization Through E3 Ubiquitin Ligase CHFR Downregulation in Human Vascular Smooth Muscle Cells

Objective—Low density lipoprotein retention and aggregation in the arterial intima are key processes in atherogenesis. Aggregated LDL (agLDL) is taken up through low-density lipoprotein receptor-related protein 1 (LRP1) by human vascular smooth muscle cells (VSMC). AgLDL increases LRP1 expression, at least in part, by downregulation of sterol regulatory element-binding proteins. It is unknown whether agLDL has some effect on the ubiquitin-proteasome system, and therefore on the LRP1 receptor turnover. The objective of this study was to analyze the effect of agLDL on the degradation of LRP1 by the ubiquitin-proteasome system in human VSMC. Methods and Results—Human VSMC were isolated from the media of human coronary arteries. Ubiquitinylated LRP1 protein levels were significantly reduced in human VSMC exposed to agLDL (100 μg/mL) for 20 hours (agLDL: 3.70±0.44 a.u. versus control: 9.68±0.55 a.u). Studies performed with cycloheximide showed that agLDL prolongs the LRP1 protein half life. Pulse-chase analysi.
Energy and Transmission Efficiency Enhancement in Passive Optical Network Enabled Reconfigurable Fronthaul Supporting Smart Homes

Smart home technologies are growing actively all around the world. As a result, great pressures are imposed on internet of things networks by dynamic traffic and plenty of devices. The passive optical network is considered one of the most promising fronthaul technologies. In particular, the time and wavelength division multiplexing passive optical network has shown the advantage of high capacity and received attention recently. In support of internet of things networks, the energy and transmission efficiency has emerged as an important issue on the time and wavelength division multiplexing passive optical network enabled fronthaul networks. In this paper, we try to enhance the energy and transmission efficiency of the time and wavelength division multiplexing passive optical network enabled reconfigurable fronthaul. Fronthaul links’ load balancing is also taken into consideration. An integer non-linear programming model is employed to formulate the joint optimization problem. We also provide an adaptive genetic algorithm-based approach with fast convergence. The simulation results show that the active units of fronthaul can be dynamically switched on/off with the traffic variation and a significant energy saving is achieved. In addition, the maximum transmission efficiency increases by 87% with integer non-linear programming method in off-peak periods.
Differences in Motion Accuracy of Baduanjin between Novice and Senior Students on Inertial Sensor Measurement Systems

This study aimed to evaluate the motion accuracy of novice and senior students in Baduanjin (a traditional Chinese sport) using an inertial sensor measurement system (IMU). Study participants were nine novice students, 11 senior students, and a teacher. The motion data of all participants were measured three times with the IMU. Using the motions of the teacher as the standard motions, we used dynamic time warping to calculate the distances between the motion data of the students and the teacher to evaluate the motion accuracy of the students. The distances between the motion data of the novice students and the teacher were higher than that between senior students and the teacher (p < 0.05 or p < 0.01). These initial results showed that the IMU and the corresponding mathematical methods could effectively distinguish the differences in motion accuracy between novice and senior students of Baduanjin.
Morphological vs. DNA metabarcoding approaches for the evaluation of stream ecological status with benthic invertebrates: Testing different combinations of markers and strategies of data filtering

Macroinvertebrate assemblages are the most common bioindicators used for stream biomonitoring, yet the standard approach exhibits several time‐consuming steps, including the sorting and identification of organisms based on morphological criteria. In this study, we examined if DNA metabarcoding could be used as an efficient molecular‐based alternative to the morphology‐based monitoring of streams using macroinvertebrates. We compared results achieved with the standard morphological identification of organisms sampled in 18 sites located on 15 French wadeable streams to results obtained with the DNA metabarcoding identification of sorted bulk material of the same macroinvertebrate samples, using read numbers (expressed as relative frequencies) as a proxy for abundances. In particular, we evaluated how combining and filtering metabarcoding data obtained from three different markers (COI: BF1‐BR2, 18S: Euka02 and 16S: Inse01) could improve the efficiency of bioassessment. In total, 140 taxa were identified based on morphological criteria, and 127 were identified based on DNA metabarcoding using the three markers, with an overlap of 99 taxa. The threshold values used for sequence filtering based on the “best identity” criterion and the number of reads had an effect on the assessment efficiency of data obtained with each marker. Compared to single marker results, combining data from different markers allowed us to improve the match between biotic index values obtained with the bulk DNA versus morphology‐based approaches. Both approaches assigned the same ecological quality class to a majority (86%) of the site sampling events, highlighting both the efficiency of metabarcoding as a biomonitoring tool but also the need for further research to improve this efficiency.
SARS-CoV-2 Cysteine-like Protease Antibodies Can Be Detected in Serum and Saliva of COVID-19–Seropositive Individuals

Key Points SARS-CoV-2 protease (Mpro) stimulates a strong Ab response. Detection of Mpro Abs in serum or saliva can provide a new diagnostic tool. Currently, there is a need for reliable tests that allow identification of individuals that have been infected with SARS-CoV-2 even if the infection was asymptomatic. To date, the vast majority of the serological tests for SARS-CoV-2–specific Abs are based on serum detection of Abs to either the viral spike glycoprotein (the major target for neutralizing Abs) or the viral nucleocapsid protein that is known to be highly immunogenic in other coronaviruses. Conceivably, exposure of Ags released from infected cells could stimulate Ab responses that might correlate with tissue damage and, hence, they may have some value as a prognostic indicator. We addressed whether other nonstructural viral proteins, not incorporated into the infectious viral particle, specifically the viral cysteine-like protease, might also be potent immunogens. Using ELISA tests, coating several SARS-CoV-2 proteins produced in vitro, we describe that COVID-19 patients make high titer IgG, IgM, and IgA Ab responses to the Cys-like protease from SARS-CoV-2, also known as 3CLpro or Mpro, and it can be used to identify individuals with positive serology against the coronavirus. Higher Ab titers in these assays associated with more-severe disease, and no cross-reactive Abs against prior betacoronavirus were found. Remarkably, IgG Abs specific for Mpro and other SARS-CoV-2 Ags can also be detected in saliva. In conclusion, Mpro is a potent Ag in infected patients that can be used in serological tests, and its detection in saliva could be the basis for a rapid, noninvasive test for COVID-19 seropositivity.
High dimensional, robust, unsupervised record linkage

Abstract We develop a technique for record linkage on high dimensional data, where the two datasets may not have any common variable, and there may be no training set available. Our methodology is based on sparse, high dimensional principal components. Since large and high dimensional datasets are often prone to outliers and aberrant observations, we propose a technique for estimating robust, high dimensional principal components. We present theoretical results validating the robust, high dimensional principal component estimation steps, and justifying their use for record linkage. Some numeric results and remarks are also presented.
Leptomeningeal Carcinomatosis: Challenges in Diagnosis and Treatment

Leptomeningeal carcinomatosis occurs in approximately 3% to 5% of all cancer patients. Leptomeningeal metastasis from primary breast cancer is the most common etiology for all leptomeningeal diseases. Triple-negative breast cancer has significantly higher metastasis rates and a poorer prognosis compared to hormone-positive breast cancer. Leptomeningeal carcinomatosis is often difficult to diagnosis and can affect any level of the central nervous system. Imaging and CSF analysis often allows for the diagnosis of leptomeningeal diseases. However, once leptomeningeal metastasis is confirmed prognosis is often poor and treatment options remain limited. This case report and literature review is meant to highlight an uncommon late-stage complication of breast cancer because early diagnosis and treatment significantly impacts morbidity and mortality.
Totally laparoscopic total gastrectomy with technique of functional end-to-end esophagojejunostomy by linear stapler without previous resection of the esophagus and jejunum

Gastric cancer is popular in the world including Vietnam. Despite there is a variety of treatments for gastric cancer, surgery is the most effective approach. Azagra is the first one who conducted the laparoscopic total gastrectomy in 1993. Until now, this approach was widely applied and benefited patients. Its advantages include the small scar, mild pain after surgery, little blood loss, a fast time for recovery, low frequency of incidents and complications after surgery, good quality of life and ABSTRACT
Sensitivity of the Edinburgh Criteria for Lobar Intracerebral Hemorrhage in Hereditary Cerebral Amyloid Angiopathy

Background and Purpose: The Edinburgh computed tomography and genetic criteria enable diagnosis of cerebral amyloid angiopathy (CAA) associated lobar intracerebral hemorrhage (ICH) but have not been validated in living patients. We assessed the sensitivity of the Edinburgh criteria in patients with acute lobar ICH due to Dutch-type hereditary CAA; a genetic and pure form of CAA. Methods: We retrospectively analyzed computed tomography-scans from a cohort of consecutive Dutch-type hereditary CAA patients who presented with ≥1 episode(s) of acute lobar ICH at the Leiden University Medical Center. Presence of subarachnoid hemorrhage (SAH) and finger-like projections (FLP) were determined. Association of SAH and FLP with ICH volume was analyzed using multivariate linear regression. Results: We included 55 Dutch-type hereditary CAA patients (mean age 56 years, 55% men) with a total of 107 episodes of acute lobar ICH. SAH was present in 82/107 (76%) and FLP in 62/107 (58%), resulting in a sensitivity of 76% for SAH and 58% for FLP. In 56 (52%), both markers were present. Nineteen (18%) lobar ICH showed no SAH extension or FLP. ICH volume was significantly associated with presence of SAH (median volume 4 versus 28 mL; P=0.001) and presence of FLP (median volume 7 versus 39 mL; P<0.001). With an ICH volume of ≥40 mL, the sensitivity of the presence of both SAH and FLP was >81% (95% CI, 70%–92%), whereas in ICH volumes <15 mL the sensitivity was <50%. Conclusions: The computed tomography-based Edinburgh criteria seem to be a sensitive diagnostic test for CAA-associated lobar ICH, although they should be used with caution in small-sized lobar ICH.
Biomechanical Response to External Biofeedback During Functional Tasks in Individuals with Chronic Ankle Instability.

CONTEXT Altered biomechanics displayed by individuals with chronic ankle instability (CAI) is a potential cause for recurring injuries and posttraumatic osteoarthritis. Current interventions are unable to modify aberrant biomechanics leading research efforts to determine if real-time external biofeedback is capable of producing changes. OBJECTIVE Determine real-time effects of visual and auditory biofeedback on functional task biomechanics in individuals with CAI Design: Crossover study Setting: Laboratory Patients or Other Participants: Nineteen physically active adults with CAI (23.95±5.52 years; 168.87±6.94 cm; 74.74±15.41 kg, female=12) volunteered. INTERVENTION Participants randomly performed single-leg static balance, step-downs, lateral-hops, and forward-lunges during a baseline and two biofeedback conditions. Auditory biofeedback was given through a pressure sensor placed under the lateral foot connected to a buzzer eliciting a noise when pressure exceeded the set threshold. Visual biofeedback was given through a cross-line laser secured to the dorsum of the foot. Cues given during the biofeedback conditions were used to promote proper biomechanics during each respective task. MAIN OUTCOME MEASURE(S) Location of center of pressure (COP) data points during balance with eyes-open and closed during each condition. Plantar pressure during functional tasks were extracted in the lateral column of the foot. Secondary outcomes of interested were COP area and velocity, time-to-boundary during static balance, and additional plantar pressure measures. RESULTS Both biofeedback conditions reduced COP in the anterolateral quadrant while increasing COP in the posteromedial quadrant of the foot during eyes open balance, the auditory condition produced similar changes during eyes closed trials. Auditory biofeedback increased heel pressure during step-downs, while decreasing lateral forefoot pressure-time integral during lunges. Visual biofeedback increased lateral heel pressure and increased lateral heel and midfoot pressure-time integral during hops. CONCLUSIONS Real-time improvements in balance strategies were observed during both external biofeedback conditions. Visual and auditory biofeedback appear to effectively moderate different functional task biomechanics.
Entrepreneurial Nurses in the Literature: A Systematic Literature Review.

AIM This study explores the main characteristics of entrepreneurial nursing. BACKGROUND Nurses have acted entrepreneurially in a multitude of ways since the very beginning of the nursing profession. Still, it is unclear what it means for a nurse to be entrepreneurial. Entrepreneurial nurses are often described inconsistently, by non-inclusive terms not founded in research. There is a need to examine the essence of entrepreneurial nursing roles. EVALUATION I conducted the research as a systematic literature review of 647 documents. Analysing empirical descriptions of entrepreneurial nurses' employment status, contexts, knowledge, activities, barriers and motivations led to the description of two entrepreneurial nursing roles. KEY ISSUES The employment status of entrepreneurial nurses represent an important difference in their activities, challenges and motivations. CONCLUSIONS Entrepreneurial nurses may act as nurse entrepreneurs or nurse intrapreneurs. These roles are empirically rooted in the field of nursing, and theoretically rooted in the field of entrepreneurship. IMPLICATIONS FOR NURSING MANAGEMENT Entrepreneurial nurses are influenced by the support they get and the challenges they face in the healthcare system. Knowing the characteristics of entrepreneurial nurses are important for nurse managers, as they lead employees who may become nurse entrepreneurs or nurse intrapreneurs.
R-loops at centromeric chromatin contribute to defects in kinetochore integrity and chromosomal instability in budding yeast

R-loops, the byproduct of DNA–RNA hybridization and the displaced single-stranded DNA (ssDNA), have been identified in bacteria, yeasts, and other eukaryotic organisms. The persistent presence of R-loops contributes to defects in DNA replication and repair, gene expression, and genomic integrity. R-loops have not been detected at centromeric (CEN) chromatin in wild-type budding yeast. Here we used an hpr1∆ strain that accumulates R-loops to investigate the consequences of R-loops at CEN chromatin and chromosome segregation. We show that Hpr1 interacts with the CEN-histone H3 variant, Cse4, and prevents the accumulation of R-loops at CEN chromatin for chromosomal stability. DNA–RNA immunoprecipitation (DRIP) analysis showed an accumulation of R-loops at CEN chromatin that was reduced by overexpression of RNH1 in hpr1∆ strains. Increased levels of ssDNA, reduced levels of Cse4 and its assembly factor Scm3, and mislocalization of histone H3 at CEN chromatin were observed in hpr1∆ strains. We determined that accumulation of R-loops at CEN chromatin contributes to defects in kinetochore biorientation and chromosomal instability (CIN) and these phenotypes are suppressed by RNH1 overexpression in hpr1∆ strains. In summary, our studies provide mechanistic insights into how accumulation of R-loops at CEN contributes to defects in kinetochore integrity and CIN.