Miracle-dz/UniFAR-FTUs huggingface dataset:
Serotype variation among infectious bronchitis viral isolates taken from several areas of Java

Infectious bronchitis (IB) is an acute highly contagious viral respiratory disease of poultry caused by virus belongs to the family of Coronaviridae. The virus consist of many serotypes with low level of cross-protectivity among serotypes. Field data showed that the outbreaks of IB were frequently reported in chicken flocks, although vaccinations against the disease have been practiced. Hence, the study on serotype relationship among isolates of the viruses is essentially required. The aim of this study was to isolate and characterize IB viruses from chicken flocks in some areas of Java. Isolation of the virus was carried out in nine-day old embrionated chicken eggs and identified by means of agar gel precipitation (AGP) tests against standard antisera to IB virus. The serotypes of the IB viral isolates were determined by cross-neutralization tests in nine day old embryonated chicken eggs using r value derived from homologous and heterologous serum titres as criteria. This study obtained 12 IB viral isolates which were identified on the basis of the ability to cause lesions in chicken embryos and positive to agar gel presipitation test against standard positive antiserum to the virus. Based on the cross-neutralization tests in embryonated chicken eggs, isolate I.9 was formed to have relationship closed to Mass-41 serotype, while I.2, I. 3, and I.7 isolates were closely to the serotype of Con-46. Virus isolates (I.5, I.14, I.24, and I.25) were decided to have no serotype relationships to either Mass-41 or Con-46 serotype. Since the I.5, I.14, I.24 and I.25 isolates were not neutralized by antisera against the previous identified local infectious bronchitis viral isolates, and that were considered to be distinct serotype to the previously identified local IB viral isolates. Key words: Infectious bronchitis, virus, embryonated egg, cross neutralization test.
A Conceptual Model of the Relationship between Organizational Structure, Organizational Learning, and Organizational Innovativeness in Malaysian Higher Education Institution

In this era of globalization, creativity and innovation have become integral in the business world and without these elements, a business might not be able to maintain its place in the market. In this regard, organizations should acquire new knowledge and skills, as well as come out with brilliant can help them to maintain and improve their position in today’s highly competitive market. In this study, a concentrated evaluation of past studies on the relationship between organizational structure, organizational learning and organizational innovativeness has been conducted to find and fill the logical research gap to help companies deal with external opportunities and threats. In light of the dynamic-abilities hypothesis, this review will fill the gap between the constructs of competitive advantage and propose a conceptual framework which can contribute towards the improvement of the existing literature.
Herramienta de ayuda diagnóstica basada en redes neuronales para analizar angiogramas reticulares implementada en el Instituto para Niños Ciegos y Sordos

Las redes neuronales artificiales son una herramienta computacional que permite realizar aplicaciones de manera automatica que de momento solo podian ser realizadas por humanos. Esta tecnologia lidera el campo de la vision por computadora y los procesos de automatizacion. En este trabajo de grado se busca la implementacion de una red neuronal para resolver un problema de clasificacion logistica, la cual se puede entender como la probabilidad de que una entrada pertenezca a una clase en particular, esto, con el fin de suplir una necesidad identificada por el autor en una institucion sin animo de lucro. En la institucion se realizan examenes de angiografia reticular de manera diaria y el acumulo de resultados se vuelve un trabajo tedioso para los medicos y a su vez el tiempo de esperar para la entrega de los resultados es considerable. Asi pues, se propuso el diseno de una herramienta de ayuda diagnostica que permite clasificar las angiografias reticulares con base a la probabilidad de ser patologica o no patologica; con el fin de entregar a los medicos en orden de mayor probabilidad patologica y tratar de disminuir el tiempo de intervencion a los pacientes con alta probabilidad de patologia. Para el desarrollo de la herramienta se uso el lenguaje de programacion Python y la libreria de redes neuronales Tensorflow y Keras. Fue necesario la creacion de una base de datos con las imagenes angiografica proporcionadas por la institucion para el proceso de entrenamiento de la red neuronal. Posteriormente, se propusieron tres alternativas viables de redes neuronales artificiales que tuviesen la capacidad de procesar la informacion angiografica; lo siguiente fue entrenar y validar cada uno de los modelos y comparar su desempeno en datos no vistos por medio de la construccion de una matriz de confusion. Finalmente, se eligio el modelo como mejor resultado en la matriz de confusion y se realizo el deployment del modelo por medio de una GUI que permite la interaccion con el usuario y la herramienta de ayuda diagnostica
Measuring in vivo oxidative damage : a practical approach

CHROMATOGRAPHIC PROCEDURES.Lipid Peroxidation Determination by HPLC (R. Bevan). Measurement of Oxidized Bases in DNA and Biological Fluids by Gas Chromatography Coupled to Mass Spectrometry (T. Douki, et al.). The Measurement of Protein Oxidation by HPLC (H. Griffiths, et al.). MEASUREMENT OF 8-OXO DEOXYGUANOSINE.Measurement of 8-Oxo-2' -deoxyguanosine in Cellular DNA by High Performance Liquid Chromatography-Electrochemical Detection (M. Evans). Immunochemical Detection of 8-Oxodeoxyguanosine in DNA (M. Cooke & K. Herbert). Urinary Measurement of 8-OxodG (8-Oxo-2' -deoxyguanosine) (H. Poulsen, et al.). CELLULAR-BASED METHODS.Measurement of Oxidative DNA Damage Using the Comet Assay (A. Collins). Measuring Oxidative DNA Damage by Alkaline Elution (M. Pflaum & B. Epe). MOLECULAR-BASED ASSAYS.A 32P-Postlabelling Protocol to Measure Oxidative DNA Damage (G. Jones & M. Weinfeld). Mapping Reactive Oxygen-Induced DNA Damage at Nucleotide Resolution (H. Rodriguez & S. Akman). ANTIOXIDANT ACTIVITY.Measurements of Plasma Antioxidant Activity (S. Maxwell). Index.
Gut regulatory peptides as mediators of gastrointestinal tract growth, motility and development of secretion in young ruminants

In mammalian species, the prenatal development of foetal tissues is programmed to result in the organization of different functions of the organism. During development, non-genetic factors are involved and act through regulatory substances. It is frequently said that the GIT represents “the largest endocrine organ in the body”. Most of these substances depend on an intricate regulatory complex increasingly recognized as a unique system having multiple aspects, including hormonal, nervous and immune ones. The link in this unique system comprises a number of substances whose task is to translate the signals. Among them, the gut regulatory peptides are considered to be substances that play a pivotal role in digestive functions. This review will deal with recent advances in the regulation of digestive functions in which the gut regulatory peptides are involved during development in calf i.e., during foetal life, after birth, during maintenance at the preruminant stage, and at weaning. This report will be devoted in particular to gut regulatory peptides as mediators of gastrointestinal (GI) growth, motility and development of secretion and adaptations. Increasing knowledge about the biological functions and mechanisms of gut regulatory peptide actions could help in managing the growth and maturation of the gastrointestinal tract (GTI), to better optimize digestive motility and productions. It is particularly important in the young to help them to better pass through such critical stages as the neonatal and weaning periods.
Building a scientific workbench in Pharo

Over the last years, the need for better user interfaces in scientific computing has become apparent by the enormous growth in popularity of computational notebooks. However, the user experience provided by these notebooks is very limited compared to the live programming environments of Smalltalk, and first studies on how they are actually used in research studies point out various weaknesses. We present the first steps we have taken towards building a scientific workbench in Pharo, based on the Glamorous Toolkit, and outline future developments towards that goal.
Bacterial amyloids promote type I interferon production and accelerate autoimmunity (BA6P.124)

Infections can be pathogenic in systemic lupus erythematosus (SLE). Curli is a bacterial amyloid part of enteric bacterial biofilms, like those of E. coli and Salmonella. We have recently found that curli fibers form highly immunogenic composites with DNA within the biofilm. Here we studied the immune response to curli-DNA complexes in the context of murine SLE. For in vitro studies, we stimulated bone marrow-derived dendritic cells (cDCs) from WT and lupus-prone mice with curli amyloid from S. Typhimurium biofilms. A dose titration of curli amyloid induced robust proinflammatory cytokine and type I-IFN responsive gene expression in cDCs. Using synthetic curli peptide, we found that curli and nucleic acids synergize to activate cDCs. We injected in vivo pre-diseased lupus-prone NZB/W-F1 mice i.p. with curli-DNA complexes and found that they accelerate disease, inducing high titers of anti-dsDNA and anti-chromatin autoantibodies. Curli-DNA complexes induced autoantibodies also in non-autoimmune C57BL/6 mice. Finally, we infected NZB/W-F1 mice i.p. with curli-expressing or curli mutant E. coli or Salmonella. Curli-competent bacteria triggered far higher autoantibody titers than curli mutant strains. In conclusion, biofilm-derived curli-DNA complexes are potent activators of DCs and can rapidly break tolerance and accelerate autoimmunity in mice, suggesting that bacterial amyloids and biofilm-based bacterial infections are important environmental triggers in lupus.
Differential gene expression in Melipona scutellaris (Hymenoptera, Meliponini): effect of juvenile hormone III

The objective of the present study was to determine by differential display reverse transcriptase–polymerase chain reaction (DDRT-PCR) the effects of juvenile hormone (JH) III applied during the late larval 3 (L3) phase on gene expression in Melipona scutellaris. A temporal window of expression of feminizing genes exists during the late L3 and pre-defecating larval phases when these genes can be turned on or off by the action of JH, which is able to mediate the differentiation of female larvae into queens. Combination of the HT11A-AP4 primers revealed differential expression in L3 individuals treated with JH III for 1 h, with weak expression of the transcript, while intense expression was observed for controls and individuals treated for 4 h. Combination of the HT11G-AP4 and HT11G-AP5 primers showed suppression of the gene products for each primer combination in 1-h treated larvae compared to untreated control individuals of the same age and individuals treated for 4 h. Differential gene expression was also observed during development. These results demonstrate that the JH III may suppress or alter gene expression profiles during phase L3 of M. scutellaris.
Knowledge Management Practices in New Public Universities in Saudi Arabia

Knowledge management (KM) is the creation, sharing, and application of information. KM is a leading strategy among business organisations that is relativity new to the educational fields, including higher education. We argue that KM assists new public universities in reforming their leadership and pedagogical activities and overcoming social economic challenges. This study explores KM in a 10-year-old public university in Saudi Arabia by analysing 212 survey responses to assess the university’s readiness for KM, including acquiring a skilled workforce, strengthening the organisational structure and culture, and providing technological support. The responses indicated significant relationships between KM practices and all three-readiness areas, as well as between KM and education and gender. The findings suggest that successful implementation of KM requires support in many areas.
Organic and inorganic solutes contents in cowpea under salt stress at different phenological stages.

This study aimed to evaluate the application of saline and/or water, alternately, at different developmental stages of cowpea cv. Epace 10. The experiment was conducted in a greenhouse at Embrapa Agroindustria Tropical. The experimental design was in randomized blocks with six treatments and four replications. The plants were subjected to irrigation with water or saline solution, according to following treatments: 1. irrigation with water until the end of phase III, 2. irrigation with saline water until the end of phase III, 3. irrigation with water until the end of phase I and with saline water until the end of Phase III, 4. irrigation with saline water until the end of phase I and with water until the end of phase III, 5. irrigation with water until the end of phase II and with saline water until the end of phase III, 6. irrigation with saline water until the end of phase II and with water until the end of phase III, when the data were collected and analysed. The chloride content of water stressed plants was higher in leaves and that of sodium was higher in stems. The content of carbohydrate of the roots was increased by saline water treatment until phase III. The salinity increased levels of N-amino solutes in leaves and decreased in roots. The protein content decreased both in leaves and roots. The joint analysis of data showed a higher sensitivity of this crop in phase III (from flowering to fruiting).
Construção de indicadores de produtividade da mão de obra com base no modelo de estratificação

A presenca de mao de obra com alta produtividade desempenha um papel significativo na realizacao de projetos. Dispostos a melhorar a eficiencia e a produtividade nos canteiros de obras, diversos esforcos tem sido realizados pelas empresas da industria da construcao civil de edificacoes no Brasil. Contudo, essa boa pratica ainda nao faz parte da rotina de grande parte das empresas de micro e pequeno porte (MPEs). O presente trabalho propoe uma alternativa para a gestao da produtividade da mao de obra, adaptada ao contexto das empresas especializadas de servicos de execucao (EEEs), que atuam na execucao dos subsistemas das edificacoes. Nesse contexto, o objetivo do trabalho consiste em apresentar um metodo de avaliacao da produtividade, baseado no “Modelo de Estratificacao” e na Razao Unitaria de Producao (RUP). O metodo foi adaptado e aplicado em duas obras reais de construcao de edificios. Foram investigadas as atividades que potencialmente influenciaram a produtividade da mao de obra e determinado o conjunto de indicadores de produtividade para esses servicos.
Ate Poput Sacred Roof In Traditional Houses Renovating Process: The Culture Semiotic Perspective Of Maluku

This study examines Ate Poput as one of local culture form that has a significant meaning, especially in renovating traditional houses process. The qualitative analysis with explorative approach method was used. The results show that Ate Poput is interpreted as a spare for the integration genealogy the process of chanting indicates that the involvement of all families, mainly those genealogies exist of the family does not only strengthen the sense of togetherness in carrying out the procession, but the important thing is to re-establish live of families separated for a long time because of different activities, both residential and religious differences. As a marker of family safety, the renovating an old house with an Ate Poput procession believed a medium that can protect families, especially those who will occupy old houses. Ate poput procession as one of the instruments of sustainability of local culture, this procession becomes a guide and learning for domestic children despite absorbing high social energy both in terms of energy time, and budget. As part of an effort to preserve local culture, the procession of Ate poput is still protected because by maintaining the sustainability of the local culture, at the same time respecting the ancestral traditions finds its momentum.
Examining the Self-Harm and Suicide Contagion Effects of the Blue Whale Challenge on YouTube and Twitter: Qualitative Study

Background Research suggests that direct exposure to suicidal behaviors and acts of self-harm through social media may increase suicidality through imitation and modeling, particularly in more vulnerable populations. One example of a social media phenomenon that demonstrates how self-harming behavior could potentially be propagated is the blue whale challenge. In this challenge, adolescents and young adults are encouraged to engage in self-harm and eventually kill themselves. Objective This paper aimed to investigate the way individuals portray the blue whale challenge on social media, with an emphasis on factors that could pose a risk to vulnerable populations. Methods We first used a thematic analysis approach to code 60 publicly posted YouTube videos, 1112 comments on those videos, and 150 Twitter posts that explicitly referenced the blue whale challenge. We then deductively coded the YouTube videos based on the Suicide Prevention Resource Center (SPRC) safe messaging guidelines as a metric for the contagion risk associated with each video. Results The thematic analysis revealed that social media users post about the blue whale challenge to raise awareness and discourage participation, express sorrow for the participants, criticize the participants, or describe a relevant experience. The deductive coding of the YouTube videos showed that most of the videos violated at least 50% of the SPRC safe and effective messaging guidelines. Conclusions These posts might have the problematic effect of normalizing the blue whale challenge through repeated exposure, modeling, and reinforcement of self-harming and suicidal behaviors, especially among vulnerable populations such as adolescents. More effort is needed to educate social media users and content generators on safe messaging guidelines and factors that encourage versus discourage contagion effects.
A Study on Relationship Between Sleep Time and HbA1c in Korean Adult Female

The purpose of this study was to determine the relationship between glycated hemoglobin and sleep time in each age group of Korean females. The candidates of study subjects were 16, 277 females from 7th National Health and Nutrition Survey (2016-2017) and raw data of 6,266 females were included in final analysis after. For the analysis, complex sample chi-square test, General Linear Model (GLM), and complex sample logistic model were performed. Results: The sleep time showed statistically significant difference in adolescent and middle-aged group, however, this significance survived only in middle-aged group after applying covariates, indicating that the longer sleep time is associated with the higher risk of diabetes mellitus. It is expected that, based on these results, the monitoring of sleep time and glycated hemoglobin is used in screening diabetes mellitus complications and other various health problems.
Characterising the molecular heterogeneity within human inhibitory interneurons, at single-cell resolution

Inhibitory interneurons are a heterogeneous class of GABAergic neurons, classified into discrete subtypes based on the expression of molecular markers, physiology, morphology and connectivity. However, the mechanism of interneuron diversification is poorly understood. hPSC-derived interneurons may help solve the mystery, by providing an unrestricted trajectory of interneuron differentiation, which is not confounded by species differences. Although, how well the model recapitulates native development remains under scrutiny. Moreover, the full extent of interneuron subtype diversity that can be achieved in vitro is unclear. Here an evaluation of hPSC model of interneuron differentiation has been presented. To tease apart the molecular heterogeneity, longitudinal single-cell RNA sequencing was conducted. The analysis revealed a heterogeneous MGE-like enriched progenitor population that differentiated into a diverse population of interneuron-like cells. On examining the authenticity of the model, using a novel cross-comparison to the native human MGE (10WPC-15WPC), encouraging similarities were found, especially between post-mitotic hPSC-derived interneurons and 15WPC MGE. However, several shortcomings of the model were also indicated. First, under-representation of specific genes encoding for transcription factors. Second, absent or low expression of late MGE markers and subtypes. Enforced expression of the under-expressed transcription factors may provide a means to enrich the interneuron-like population with these subtypes. Furthermore, the molecular resemblance to foetal-derived interneurons was reflected in the immature intrinsic and evoked neuronal responses of hPSC-derived interneurons, recorded using whole-cell patch clamp. Nevertheless, monosynaptic viral tracing demonstrated the capability of the interneuron-like cells to make synaptic connections, which (although not extensive) may be enhanced in a co-culture system. Overall, the interneuron differentiation of hPSC holds promise as a neurodevelopmental model. The novel characterisation of hPSC-derived interneurons and human MGE, at single-cell resolution, has simultaneously v revealed the limitations and refinements necessary to generate more authentic and defined interneuron subtypes in vitro. Thereby, widening the scope and validity of the model.