Miracle-dz/UniFAR-FTUs huggingface dataset:
Design and implementation of an indoor test bench for validation of solar MPPT algorithms

As a solution to the problem of increasing deficit of electrical energy, the use of renewable energy sources is increasing. Application of renewable energy resources in electricity generation system is done in a variety of configurations such as standalone photovoltaic plants, standalone wind power plants, Microgrid system, etc. The system described and developed in this paper, aims to realize a low cost, reliable and robust test bench for testing various maximum power point tracking algorithms. This paper introduces photovoltaic simulator implemented using simple controllable DC power supply and PC, with the capability of simulating wide range of irradiance and temperature conditions. The system consists of PWM controlled boost chopper, microcontroller with inbuilt real time control capability and respective sensors for implementation of various algorithms. The proposed system is a part of COEP-Microgrid, a project initiated by the students of College of Engineering, Pune, which aims at establishing a Microgrid in the college campus. Perturb & Observe algorithm has been implemented on this test bench to check and verify the ability of the system.
omparison of monocyte-derived dendritic cells from colorectal cancer patients , on-small-cell-lung-cancer patients and healthy donors

Dendritic cells (DCs) are bone marrow-derived professional antigen presenting cells. Due to their role as potent inducers of immune responses, these cells are widely used as adjuvant in experimental clinical settings for cancer immune therapy. We have developed a DC-based vaccine using autologous blood monocytes loaded with allogeneic tumor cell lysate rich in cancer/testis antigens. This vaccine has at present been tested for activity in three phase II clinical trials including two cohorts of patients with advanced colorectal cancer (CRC) and one cohort of patients with advanced non-small-cell-lung-cancer (NSCLC). In the present paper we retrospectively compare the maturation profile based on surface marker expression on DCs generated from the three patient cohorts and between cancer patient cohorts and a cohort of healthy donors. Vaccines were generated under cGMP conditions and phenotypic profiles of DC were analyzed by flow cytometry and the obtained data were used as a basis to set guideline values for our quality control of GMP produced DC vaccines. Each vaccine batch was analyzed for the expression of the surface maturation and differentiation molecules CD14, CD1a, CD83, CD86, MHC class II and CCR7, and the optimal expression pattern is considered as CD14low, CD1a, CD83high, CD86high, MHC class IIhigh and CCR7high. In accordance with data from other studies including other types of cancer patients, especially breast cancer patients, we found that the maturation status of the DC batches depends on cancer type and atus o correlates with clinical st
Performance, blood and ruminal characteristics of cows receiving monensin and a magnesium supplement.

Forty-eight Angus and Hereford cows were used in a completely random design with a 2 X 2 factorial arrangement of treatments to evaluate the effects of monensin and two sources of Mg on performance, ruminal characteristics and mineral status of mature beef cows. Cows were fed wheat straw treated with NH3 during late gestation and straw treated with NH3 plus bromegrass haylage after calving along with a protein supplement that contained either MgO (7.5 g/kg) or MgSO4 (11 g/kg). One half of the cows received monensin (1 g/kg) in their protein supplement. During the precalving period, MgO without monensin increased (P less than .01) plasma Mg compared with Mg in plasma of cows receiving MgSO4 or MgO with monensin. Magnesium oxide also increased cow weight gains during late gestation (P less than .05) compared with weight gains of cows supplemented with MgSO4, but magnesium source had no effect on weight gain postcalving. Plasma glucose was increased by monensin (P less than .05) and by MgSO4 (P less than .01) during early lactation. Monensin increased the proportion of propionate before (P less than .05) and after (P less than .10) calving in the ruminal fluid of cows. Monensin also increased (P less than .05) cow weight gain precalving and tended to decrease (P less than .10) milk fat in lactating cows. Calf weight gains were not affected by treatment of dam. Magnesium oxide appeared to have higher Mg bioavailability than MgSO4 for cows precalving, but Mg bioavailability was not different postcalving. Monensin increased cow weight gains precalving and improved feed efficiency postcalving.
Microbial Community Structure in Midgut and Hindgut of the Humus-Feeding Larva of Pachnoda ephippiata (Coleoptera: Scarabaeidae)

ABSTRACT The guts of soil-feeding macroinvertebrates contain a complex microbial community that is involved in the transformation of ingested soil organic matter. In a companion paper (T. Lemke, U. Stingl, M. Egert, M. W. Friedrich, and A. Brune, Appl. Environ. Microbiol. 69:6650-6658, 2003), we show that the gut of our model organism, the humivorous larva of the cetoniid beetle Pachnoda ephippiata, is characterized by strong midgut alkalinity, high concentrations of microbial fermentation products, and the presence of a diverse, yet unstudied microbial community. Here, we report on the community structure of bacteria and archaea in the midgut, hindgut, and food soil of P. ephippiata larvae, determined with cultivation-independent techniques. Clone libraries and terminal restriction fragment length polymorphism analysis of 16S rRNA genes revealed that the intestines of P. ephippiata larvae contain a complex gut microbiota that differs markedly between midgut and hindgut and that is clearly distinct from the microbiota in the food soil. The bacterial community is dominated by phylogenetic groups with a fermentative metabolism (Lactobacillales, Clostridiales, Bacillales, and Cytophaga-Flavobacterium-Bacteroides [CFB] phylum), which is corroborated by high lactate and acetate concentrations in the midgut and hindgut and by the large numbers of lactogenic and acetogenic bacteria in both gut compartments reported in the companion paper. Based on 16S rRNA gene frequencies, Actinobacteria dominate the alkaline midgut, while the hindgut is dominated by members of the CFB phylum. The archaeal community, however, is less diverse. 16S rRNA genes affiliated with mesophilic Crenarchaeota, probably stemming from the ingested soil, were most frequent in the midgut, whereas Methanobacteriaceae-related 16S rRNA genes were most frequent in the hindgut. These findings agree with the reported restriction of methanogenesis to the hindgut of Pachnoda larvae.
An empirical study on the impact of the gift cards' properties on the effectiveness of the gift cards promotion

According to the test that we made based on the theory of consumer behavior, we found how purchase amount threshold, gift card face value and using restriction affect the effectiveness of the gift cards promotion. Consumers' perception and purchasing wishes tend to be lower with a higher threshold, which influence the transaction value consumers have to pay to obtain the extra compensation. In addition, consumers also tend to have higher perception and willingness to pay with a higher gift card face value; because the gift card's economic benefit can change the consumer's price perception. However, the combined effect of a high threshold and a high gift card face value is going to create higher perceptions of the consumer.
The Clinical Chemistry of Chimpanzees. I. Determination of Aminotransferase Baseline Values for Hepatitis Studies

Viral hepatitis in chimpanzees produces negligible symptomatology, and serum aminotransferase changes may be minimal. To maximize the predictive value of these determinations, which are the only serum indicators available for non‐A non‐B (NANB) hepatitis infection, normal ranges for aspartate and alanine aminotransferases (AST, ALT) were examined and categorized according to age and sex. Males were found to have higher values than females, and adults higher values than juveniles. The kinetic method used and the values obtained are described. Differences in methodologies and reporting units are discussed.
Evaluation of study discontinuations with tapentadol inmmediate release and oxycodone immediate release in patients with low back or osteoarthritis pain.

OBJECTIVE To examine discontinuations due to nausea and/or vomiting or constipation with tapentadol immediate release (IR) or oxycodone IR treatment. DESIGN Post hoc analyses of a 90-day, phase 3, randomized, double-blind, flexible-dose study. SETTING United States, Canada, United Kingdom. PATIENTS Adults with moderate to severe low back or osteoarthritis (OA) pain for > or =3 months. INTERVENTIONS Tapentadol IR 50 or 100 mg or oxycodone HCI IR 10 or 15 mg every 4-6 hours as needed. MAIN OUTCOME MEASURES Adverse events and discontinuations were recorded. RESULTS Post hoc analyses included data from 679 patients receiving tapentadol IR and 170 receiving oxycodone IR (4:1 randomization). Tapentadol IR 50 or 100 mg and oxycodone HCI IR 10 or 15 mg provided similar levels of pain relief Overall, 21.2 percent of patients receiving tapentadol IR discontinued treatment for adverse events versus 31.2 percent of patients receiving oxycodone IR. The percentage of patients who discontinued for nausea and/or vomiting was significantly lower with tapentadol IR (5.9 percent) than oxycodone IR (14.7 percent; p = 0.0003); patients treated with oxycodone IR discontinued because of nausea and/or vomiting significantly earlier than with tapentadol IR (p < 0.0001). The percentage of patients who discontinued because of constipation was significantly lower with tapentadol IR (1.5 percent) than with oxycodone IR (5.9 percent; p = 0.0023); patients treated with oxycodone IR discontinued because of constipation significantly earlier versus tapentadol IR (p = 0.0003). CONCLUSIONS A lower percentage of patients discontinued because of nausea and/or vomiting or constipation with tapentadol IR versus oxycodone IR while receiving comparable pain relief suggesting tapentadol may improve the management of low back and OA pain.
Cross-Linking and Intracorneal Ring Segments--Review of the Literature.

Objectives: To review and summarize relevant studies on combined use of corneal crosslinking and intracorneal ring segments (ICRS) for the treatment of corneal ectasia. Methods: A literature search was performed using the key words “corneal collagen cross-linking” and “intra-corneal ring segments. ” Results: Crosslinking is a well-accepted treatment option for corneal ectasia and can be combined with corneal ICRS insertion as needed. The advent of crosslinking has dramatically reduced the number of corneal transplants performed for patients with keratoconus or postrefractive ectasia. Intracorneal ring segments, although flatten the corneal surface, do not prevent the progression of corneal ectasia. This is why the combination of crosslinking and ICRS is potentially synergistic. Previous studies reported that combining these two complementary interventions has been shown to be safe and effective. Conclusions: The effects of ICRS are enhanced and stabilized by crosslinking, but the ideal combined technique is not known at present.
Ionic Mechanisms and Vectorial Model of Early Repolarization Pattern in the Surface Electrocardiogram of the Athlete

Background: The electrocardiogram (ECG) of the athlete displays particular characteristics as a consequence of both electrophysiological and autonomic remodeling of the heart that follows continued physical training. However, doubts persist on how these changes directly interact during ventricular activation and repolarization ultimately affecting surface ECG waveforms in athletes.
Structural and functional epitopes of the human adhesion receptor CD58 (LFA‐3)

CD58 (LFA‐3), a heavily glycosylated protein of 40–70 kDa, is expressed on a broad range of hematopoietic and non‐hematopoietic cells. It serves as a physiological ligand of the CD2 receptor, present on T cells and natural killer cells, and plays, thus, an important role in lymphocyte adhesion and T cell activation through CD2. Whereas several epitopes and their respective function are known for CD2, a similarly detailed characterization of CD58 is still lacking. We raised a panel of novel murine monoclonal antibodies (mAb) against recombinant human CD58 and describe here the identification of six structurally and/or functionally distinct epitopes on the CD58 molecule. All epitopes were found to be present in equal numbers on a wide range of CD58+ cells, none of them being differentially up‐regulated following cell activation or malignant transformation. Two of these epitopes represent functionally relevant sites, involved in binding of CD58 to CD2 and T cell activation via CD2. One further epitope appears to be selectively involved in CD58‐mediated activation, whereas the other three displayed no functional effects. The new mAb allow for the first time the detection of CD58 in enzyme‐linked immunosorbent assays and immunofluorescence while bound to its receptor CD2 in human serum or on freshly isolated blood cells. Finally, one mAb was found to specifically cross‐react with T11TS, the equivalent of CD58 in sheep.
The Importance of Inter-Species Cell-Cell Co-Aggregation between Lactobacillus plantarum ML11-11 and Saccharomyces cerevisiae BY4741 in Mixed-Species Biofilm Formation

Cells of Lactobacillus plantarum ML11-11, an isolate from Fukuyama pot vinegar, and the yeast Saccharomyces cerevisiae formed significant mixed-species biofilms with concurrent inter-species co-aggregation. The co-aggregation did not occur with heated or proteinase K-treated ML11-11 cells, or in the presence of D-mannose, suggesting that surface proteins of ML11-11 and mannose-containing surface substance(s) of yeast were the predominant contributing factors. Sugar fatty acid ester inhibited mixed-species biofilm formation, but did not inhibit co-aggregation, suggesting that the cell-cell adhesion and cell-polystylene adhesion are controlled by different mechanisms. Microscopic observation and microflora analysis revealed that inter-species co-aggregation plays an important role in the formation of the mixed-species biofilm.
Novel Therapeutic concepts Drug-induced arrhythmia : pharmacogenomic prescribing ?

Cardiovascular Sciences Research Centre, St George’s University of London, London SW17 0RE, UK; Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0575, USA; Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN 37232 0575, USA; and Office of Personalized Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232 0575, USA
Additive interaction between renin-angiotensin system and lipid metabolism for cancer in type 2 diabetes mellitus

This is an uncopyedited electronic version of an article accepted for publication in Diabetes. The American Diabetes Association, publisher of Diabetes, is not responsible for any errors or omissions in this version of the manuscript or any version derived from it by third parties. The definitive publisher-authenticated version will be available in a future issue of Diabetes in print and online at http://diabetes.diabetesjournals. org. Diabetes Publish Ahead of Print, published online April 28, 2009
Características del nivel de empatía hacia el paciente en internos de medicina de dos hospitales nacionales

Objetivo: Determinar las características en el nivel de empatía hacia el paciente en los internos de Medicina del Hospital Nacional “Daniel Alcides Carrión” del callao (HNDAC) y del Hospital “María Auxiliadora” (HMA) de San Juan de Miraﬂores , su relación con la edad, sexo, tipo, procedencia de la universidad y el tipo de especialidad a seguir. Materiales y métodos: Se realizó un estudio transversal, descriptivo y de correlación en 131 internos de Medicina el 2012, usando la Escala de Empatía Médica de Jeﬀerson (EEMJ). Para el análisis de cada variable independiente se usó la prueba t de Student, el ANOVA para la correlación de cada ítem y el coeﬁciente de correlación de Pearson para el análisis entre las variables y los factores de la EEMJ.Resultados:La media de empatía total fue (112.27±11.85), con un Alfa de Cronbach (0,85). Las mujeres obtuvieron mayor empatía que los varones (p>0,05). El nivel de empatía de los internos con el rango de edad entre 26-30 años fue mayor (p>0,05). El promedio de empatía de los internos de universidades públicas fue mayor (p>0,05). El promedio de empatía en los internos que desean seguir una especialidad ligada al paciente fue mayor al de los internos deseos de seguir una especialidad ligada a la tecnología. (p>0,05). El promedio de empatía en internos de Lima fue mayor (p>0,05). En el análisis de grado de relación de los ítems de la EEMJ se obtuvieron resultados relacionados a los tres factores ya establecidos en estudios anteriores. Conclusiones: El nivel de empatía encontrado se asemeja a los reducidos niveles del mismo en estudios previos realizados a estudiantes de medicina de los últimos años y el personal de salud en otros países.
Olive domestication and diversification in the Mediterranean Basin.

Olive (Olea europaea ssp. europaea) is the most important oil fruit crop in temperate areas, but the origin of the cultivated olive remains unclear. The existence of one or several domestication events in the Mediterranean Basin (MB) is still debated. We analyzed a dataset of 387 cultivated and wild accessions that were genotyped at 25 simple-sequence repeat (SSR) loci. The sample represented genetic diversity at the geographic extremes of the MB. We inferred relationships among samples and also applied approximate Bayesian computation to estimate the most probable demographic model of our samples. Cultivated olives clustered into three different gene pools (Q1, Q2 and Q3), corresponding loosely to the west, central and eastern MB, respectively. Q1 consisted primarily of accessions from southern Spain, retained the fingerprint of a genetic bottleneck, and was closely related to accessions from the eastern MB. Q2 showed signs of recent admixture with wild olives and may derive from a local domestication event in the central MB. Overall our results suggest that admixture shaped olive germplasm and perhaps also local domestication events.
REDUCTION AND CARBOXYMETHYLATION OF SOME OF THE GLIADIN WHEAT PROTEINS

Pure β-Gliadin from Maris Widgeon wheat homogeneous on gel chromatography, disc gel electrophoresis (PAGE) and purified on ion exchange chromatography was subjected to investigation for possible presence of intermolecular disulphide bonds. The protein was reduced by β mercaptoethanol followed by alkylation. Reduced and alkylated β-gliadin was then isolated after chromatography through G-75 and G-100 Sephadex gels. PAGE electrophoresis of the two halves showed two mobility bands. The molecular weight of the proteins after reduction and alkylation was the same as the original β-gliadin before it was subjected to reduction and alkylation. It was concluded that they were not two chains of the same protein but two separate proteins.
Step length adaptation for walking assistance

Lower exoskeleton has gained considerable interests in rehabilitation and health care applications, since it has the potential to help the paraplegia patients to walk again. On the control of these lower exoskeletons, the predefined gait control methods are commonly used, which requires the pilot to adjust his/her movements to follow the predefined motion trajectories. In the meanwhile, the desired trajectories are predefined from healthy persons or extrapolated from clinical gait analysis (CGA) datasets. However, in individual walking assistance situations, the exoskeleton should have the ability to adapt with variant motions of the pilot, since the pilot will change his motion in different walking situations. This paper presents a novel step length adaptation method to adapt the pilot's motion for walking assistance exoskeletons. In this paper, the exoskeleton robot is model as a special Hybrid Human-Exoskeleton Agent (HHEA), which considers both the exoskeleton and the pilot. The Dynamic Movement Primitives (DMP) is utilized to model the exoskeleton gait trajectories dynamically, in which the relationship between the gait length and Center of Mass (CoM) of HHEA is considered. In the training process, a Reinforcement Learning (RL) method is employed to update the parameters of dynamic gait model online. We demonstrate the efficiency of the proposed step length adaptation method in simulation environment as well as a lower limb exoskeleton system named as AssItive DEvice for paRaplegics (AIDER). Experimental results shows that the proposed step length adaptation method is able to adapt variant motions of the pilot during walking.
Rehabilitation robotics: a review

This article reviews various selected literature on rehabilitation robotics. The literature was obtained mainly from journals and conference proceedings of the robotic, rehabilitative or biomedical engineering associations. It has been classified into three categories: rehabilitation robot systems, evaluation and key technologies. Commercially available robots, new projects, users' experiences and requirements, and fundamental research are introduced. A comprehensive list of references is provided.
Mechanism of action of a repressor of dioxin-dependent induction of Cyp1a1 gene transcription

A dominant mutant of Hepa-1 cells, c31, expresses a repressor that prevents 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-dependent stimulation of Cyp1a1 transcription. The repressor acts via the xenobiotic-responsive elements (XREs), which are the DNA-binding sites for the aryl hydrocarbon (Ah) receptor-TCDD complex during transcriptional activation of the gene. High-salt nuclear extracts prepared from c31 cells grown with TCDD contained normal levels of the Ah receptor which bound the XRE with normal affinity, as judged by in vitro gel mobility shift assays. Furthermore, extracts prepared from these cells, grown either with or without TCDD, contained no novel XRE-binding proteins compared with extracts from wild-type Hepa-1 cells. However, in vivo genomic footprinting demonstrated that TCDD treatment leads to binding of the Ah receptor to the XREs in Hepa-1 but not mutant cells. This finding suggests that the repressor associates with the Ah receptor to prevent its binding to the XREs and that high-salt treatment either causes dissociation of the receptor/repressor complex or fails to extract the repressor from nuclei. The results underscore the importance of using both in vivo and in vitro assays for analyzing DNA-protein interactions.
G4 DNA Binding by LR1 and Its Subunits, Nucleolin and hnRNP D, A Role for G-G pairing in Immunoglobulin Switch Recombination*

The immunoglobulin heavy chain switch regions contain multiple runs of guanines on the top (nontemplate) DNA strand. Here we show that LR1, a B cell-specific, duplex DNA binding factor, binds tightly and specifically to synthetic oligonucleotides containing G-G base pairs (K D ≤ 0.25 nm). LR1 also binds to single-stranded G-rich sequences (K D ≈ 10 nm). The two subunits of LR1, nucleolin and hnRNP D, bind with high affinity to G4 DNA (K D = 0.4 and 0.5 nm, respectively). LR1 therefore contains two independent G4 DNA binding domains. We propose that LR1 binds with G-G-paired structures that form during the transcription of the S regions that is prerequisite to recombination in vivo. Interactions of donor and acceptor S regions with subunits of the LR1 could then juxtapose the switch regions for recombination.