Miracle-dz/UniFAR-FTUs huggingface dataset:
miR‐483‐3p controls proliferation in wounded epithelial cells

The mechanisms that regulate keratinocyte migration and proliferation in wound healing remain largely unraveled, notably regarding possible involvements of microRNAs (miRNAs). Here we disclose up‐regulation of miR‐483‐3p in 2 distinct models of wound healing: scratch‐injured cultures of human keratinocytes and wounded skin in mice. miR‐483‐3p accumulation peaks at the final stage of the wound closure process, consistent with a role in the arrest of “healing” progression. Using an in vitro wound‐healing model, videomicroscopy, and 5‐bromo‐2'‐uridine incorporation, we observed that overexpression of miR‐483‐3p inhibits keratinocyte migration and proliferation, whereas delivery of anti‐miR‐483‐3p oligonucleotides sustains keratinocyte proliferation beyond the closure of the wound, compared with irrelevant anti‐miR treatment. Expression profiling of keratinocytes transfected with miR‐483‐3p identified 39 transcripts that were both predicted targets of miR‐483‐3p and down‐regulated after miR‐483‐3p overexpression. Luciferase reporter assays, Western blot analyses, and silencing by specific siRNAs finally established that kinase MK2, cell proliferation marker MKI67, and transcription factor YAP1 are direct targets of miR‐483‐3p that control keratinocyte proliferation. miR‐483‐3p‐mediated down‐regulation of MK2, MKI67, and YAP1 thus represents a novel mechanism controlling keratinocyte growth arrest at the final steps of reepithelialization. —Bertero, T. , Gastaldi, C. , Bourget‐Ponzio, I. , Imbert, V. , Loubat, A. , Selva, E. , Busca, R. , Mari, B. , Hofman, P., Barbry, P., Meneguzzi, G. , Ponzio, G. , Rezzonico, R. miR‐483‐3p controls proliferation in wounded epithelial cells. FASEB J. 25, 3092‐3105 (2011). www.fasebj. org
Daños provocados por enfermedades foliares y por heladas en E. globulus I. Efecto fenotípico sobre el comportamiento productivo posterior

Eucalyptus globulus es la especie forestal de mayor area plantada en Uruguay, existiendo en la actualidad mas de 250 mil hectareas de plantaciones. Sin embargo, debido al efecto combinado de diversos factores (falta de adaptacion a las condiciones agro-ecologicas de algunas regiones, susceptibilidad a varias enfermedades y utilizacion de fuentes de semilla inadecuadas), la productividad de esta especie ha sido en general bastante inferior a lo esperado. Si bien se reconoce que los problemas sanitarios son una de las principales limitantes productivas, las perdidas economicas provocadas por diferentes enfermedades aun no han sido cuantificadas. El presente trabajo realiza un aporte en este sentido mediante el analisis del efecto que diferentes niveles de dano foliar tienen sobre parametros productivos. El estudio se realizo en un test genetico en el cual en el primer ano se cuantifico el dano provocado por enfermedades y por heladas, mientras que al tercer ano se evaluo el crecimiento en diametro y la sobrevivencia. Los resultados de dicha evaluacion permitieron comprobar que un alto nivel de dano foliar, tanto de manchas como de defoliacion, puede provocar hasta un 40% de reduccion en el crecimiento en diametro al tercer ano. A su vez, el porcentaje de mortalidad hasta el tercer ano de los arboles que presentaron altos niveles de dano foliar puede llegar al 90%. El dano provocado por heladas durante el primer invierno tambien tiene un marcado efecto sobre el comportamiento posterior, con perdidas de crecimiento en diametro para los niveles mas altos de dano de hasta un 57% y una mortalidad hasta el tercer ano que puede llegar al 80%.
QUALITY OF LIFE AND PSYCHOLOGICAL FUNCTIONING OF ICD PATIENTS

The use of the implantable cardioverter-defibrillator (ICD) for life threatening ventricular arrhythmias is standard therapy, in large part because clinical trials data have consistently demonstrated its superiority over medical treatment in preventing sudden cardiac death.1 This success prompts closer examination and refinement of quality of life (QOL) outcomes in ICD patients. Although no universal definition of QOL exists, most researchers agree that “quality of life” is a generic term for a multi-dimensional health outcome in which biological, psychological, and social functioning are interdependent.2 To date, the clinical trials demonstrating the efficacy of the ICD have focused primarily on mortality differences between the ICD and medical treatment. While the majority of the QOL data from these trials is yet to be published, many small studies are available for review and support the concept that ICD implantation results in desirable QOL for most ICD recipients.3 In some patients, however, these benefits may be attenuated by symptoms of anxiety and depression when a shock is necessary to accomplish cardioversion or defibrillation. This paper reviews the published literature on QOL and psychological functioning of ICD patients and outlines the clinical and research implications of these findings. Definitive conclusions about QOL differences between patients managed with an ICD and those treated with antiarrhythmic drugs are difficult to make in the absence of large, randomised, controlled trials. Available evidence indicates that ICD recipients experience a brief decline in QOL from baseline but improve to pre-implant levels after one year of follow up.4 The largest clinical trial data published in final form is from the coronary artery bypass graft (CABG) Patch trial which randomised patients to ICD (n = 262) versus no ICD (n = 228) while undergoing CABG surgery.5 In contrast to May and colleagues,4 data from this trial indicate …
Sampled-Data Based Average Consensus Control for Networks of Continuous-Time Integrator Agents With Measurement Noises

In this paper, sampled-data based average-consensus control is considered for networks consisting of continuous-time first-order integrator agents under a noisy distributed communication environment. The impact of the sampling size and the number of network nodes on the system performances is analyzed. The control input of each agent is based only on the information measured at the sampling instants from its neighborhood rather than the complete continuous process, and the measurement of its neighbors' states are corrupted by communication noises. By probability limit theory and the property of graph Laplacian, it is shown that for a connected network, when the sampling size is sufficiently small, the static mean square error between the individual state and the average initial states of all nodes is arbitrarily small. Furthermore, by choosing properly the consensus gains the almost sure consensus can be achieved. It is worth pointing out that an uncertainty principle of Gaussian networks is obtained, which tells us that in the case of white Gaussian noises, no matter what the sampling size is, the product of the static and transient performance indexes is always equal to or larger than a constant depending on the noise intensity, network topology and the number of network nodes.
Exhaled nitric oxide in spray painters exposed to isocyanates: effect modification by atopy and smoking

Background Isocyanate asthma is one of the most frequently identified forms of occupational asthma in industrialised countries. The underlying mechanisms have not been clarified. There is only limited information about the relationship between exhaled nitric oxide (eNO) and occupational exposure to isocyanates and asthma. Objectives To investigate the association between isocyanate exposure and eNO levels in isocyanate-exposed workers and to elucidate whether eNO acts as a marker of airway inflammation controlling for smoking and atopy in an industry-wide survey. Methods Information on estimated personal isocyanate exposure, measured eNO levels, health effects and sensitisation were analysed in 229 workers from a cross-sectional study. Univariate and multiple regression analyses were used to explore the exposure–response relationships between isocyanate exposure and eNO, stratified by smoking and atopy. Results A marginally significant exposure–response relationship was found between isocyanate exposure and eNO in atopic, non-smokers (p=0.054). eNO was significantly associated with atopy and smoking, bronchial hyper-responsiveness (BHR), work-related conjunctivitis and rhinitis after adjustment for age, gender, atopy and smoking (p<0.05). A borderline significant association was found between eNO and asthma-like symptoms after adjustment for age, gender, atopy and current smoking (p=0.055). In a small group of isocyanate-exposed workers with positive serum-specific immunoglobulin E (IgE) antibodies to hexamethylene diisocyanate (HDI), elevated eNO levels were clearly exposure related. eNO was associated with the positive specific IgG antibodies to HDI in non-atopic, non-smokers (p=0.03). Conclusions Increased eNO levels may indicate increased airway inflammation in atopic, non-smokers exposed to isocyanates especially at higher levels of isocyanate exposure.
Genetic based Local Ternary Pattern Feature Extraction for mitigating replay attacks

Genetic and Evolutionary Feature Extraction (GEFE) is a feature extraction technique that can be used to optimize feature extractors for any uniquely textured image. This technique has been extensively used with the Local Binary Pattern feature extraction algorithm for biometric recognition. This work proposes the combination of GEFE and the Local Ternary Pattern feature extraction technique. GEFE+LTP will be compared to GEFE+LBP for Iris recognition. We will then test the effectiveness of GEFE_LTP towards mitigating replay attacks.
Radiation‐induced dementia in patients cured of brain metastases

When a patient with cancer develops a brain metastasis, death is usually imminent, but aggressive treatment in some patients with limited or no systemic disease yields long-term survival. In such patients, delayed deleterious effects of therapy are particularly tragic. We report 12 patients who developed delayed complications of whole brain radiotherapy (WBRT) given as sole treatment (4 patients) or in combination with surgical resection (8 patients). Within 5 to 36 months (median, 14) all patients developed progressive dementia, ataxia, and urinary incontinence causing severe disability in all and leading to death in 7. No patient had tumor recurrence when neurologic symptoms began. Cortical atrophy and hypodense white matter were identified by CT in all. Contrast-enhancing lesions were seen in 3 patients; 2 of the lesions yielded radionecrosis on biopsy. Autopsies on 2 patients revealed diffuse chronic edema of the hemispheric white matter in the absence of tumor recurrence. Corticosteroids and ventriculoperitoneal shunt offered significant but incomplete improvement in some patients. The total dose of WBRT was only 2,500 to 3,900 cGy, but daily fractions of 300 to 600 cGy were employed. We believe that these fractionation schedules, several of which are used commonly, predispose to delayed neurologic toxicity, and that more protracted schedules should be employed for the safe and efficacious treatment of good-risk patients with brain metastases. The incidence of WBRT-induced dementia was only 1.9 to 5.1% in the 2 populations reviewed here; however, this underestimates the incidence because only severely affected patients could be identified from chart review.
The zta transactivator involved in induction of lytic cycle gene expression in Epstein-Barr virus-infected lymphocytes binds to both AP-1 and ZRE sites in target promoter and enhancer regions

The BZLF1 or zta immediate-early gene of Epstein-Barr virus (EBV) encodes a 33-kilodalton phosphorylated nuclear protein that is a specific transcriptional activator of the EBV lytic cycle when introduced into latently infected B lymphocytes. We have shown previously that the divergent EBV DSL target promoter contains two zta-response regions, one within the minimal promoter and the other in an upstream lymphocyte-dependent enhancer region. In this study, we used footprinting and gel mobility retardation assays to reveal that bacterially synthesized Zta fusion proteins bound directly to six TGTGCAA-like motifs within DSL. Four of the Zta-binding sites lay adjacent to cellular TATA and CAAT factor-binding sites within the minimal promoter, and two mapped within the enhancer region. Single-copy oligonucleotides containing these Zta-binding sites conferred Zta responsiveness to heterologous promoters. In addition, the Zta protein, which possesses a similar basic domain to the conserved DNA-binding region of the c-Fos, c-Jun, GCN4, and CREB protein family, proved to bind directly to the consensus AP-1 site in the collagenase 12-O-tetradecanoylphorbol-13-acetate response element. Cotransfection with zta also trans activated a target reporter gene containing inserted wild-type 12-O-tetradecanoylphorbol-13-acetate response element oligonucleotides. Cellular AP-1 binding activity proved to be low in latently EBV-infected Raji cells but was induced (together with the Zta protein) after activation of the lytic cycle with 12-O-tetradecanoylphorbol-13-acetate. We conclude that EBV may have captured and modified a cellular gene encoding a c-jun-like DNA-binding protein during its evolutionary divergence from other herpesviruses and that this protein is used to specifically redirect transcriptional activity toward expression of EBV lytic-cycle genes in infected cells.
Population-Based Prevalence of Abnormal Cervical Cytology Findings and Local Risk Factors in Ibadan, Nigeria: Implications for Cervical Cancer Control Programs and Human Papilloma Virus Immunization

Objective: To investigate the prevalence of abnormal cervical cytological findings and local risk factors in Ibadan, Nigeria. Study Design: All women aged ≥15 years in each household in Idikan, Ibadan, were invited to participate in a population-based study. Structured questionnaires were administered to all consenting women. Conventional cervical Papanicolaou smears obtained from sexually active women were classified using the 2001 Bethesda system. The diagnoses were correlated with sociodemographic data and risk factors. Results: Of 2,870 women aged ≥15 years estimated to live in Idikan, 1,204 sexually active women consented to pelvic examination and cervical smears. Results were available for 1,104 women (mean age: 39.8 years). Mean ages at menarche, first sexual intercourse and first pregnancy were 16.1, 20.3 and 20.7 years, respectively. Cytological results were categorized into atypical squamous cells of undetermined significance and atypical glandular cells 22 (1.99%); low-grade 43 (3.89%) and high-grade squamous intraepithelial lesions (HSIL) 17 (1.54%); invasive cancer 2 (0.18%) and normal 593 (53.8%) and reactive changes 427 (38.7%). The prevalence of epithelial abnormalities is 7.6%. Significant host-related factors in those with HSIL and invasive cancer included older age (mean 56.2 years), high parity and gravidity, lack of formal education and being divorced (p < 0.05). Conclusions: This study provides prevalence data and local risk factors for abnormal cervical cytology in a Nigerian population, which will be useful for planning future cervical cancer control programs.
[Noise Effects on Mental Health: a review of literature].

UNLABELLED Any human activity generates noise. It is considered as a risk factor for people's health. The present review of literature has assessed the impact of noise on mental health; it is summarized into four points: objective, methods, results and conclusion. OBJECTIVE The main objective of this study is to expose the actual knowledge state of noise effects on mental health after overview and critical analysis of literature to identify the acquired and shortcomings, to reflect on research direction in terms of noise pollution in the future. METHODS The literature review was conducted based on: research of keys words in articles published, research of the number of quotations of articles in Journal Citation Reports (JCR), published in web of science, research of impact factor of journals. One hundred articles were selected, after analyzing contents, items were classified into: fundamental studies (25%), experimental studies (50%), and epidemiological studies (25%). RESULTS The fundamental studies have verified the hypothesis according to which noise generates stress. Researchers have dosed hormones of stress in plasma, urine and saliva in individuals exposed to noise of different decibels. The results found were unanimous: The rates of stress hormones found, were significantly high in three liquids. This means that noise causes stress. For experimental studies, researchers have experienced the role of noise on memory, attention and performance. Human subjects were exposed to different decibels to assess level of disruption to their memory, attention, and performance. The results revealed that noise disturbs memory, distracts attention and decreases performance. Experimental studies are the most abundant and constitute 50% of the current literature review.The epidemiological studies have evaluated the intellectual performance of students in schools located in noisy environments and residents in areas surrounding airports, railways and highways. RESULTS have revealed that students in schools located in noisy environments have presented cognitive impairment. CONCLUSION This review of literature on noise effects on mental health lead to the following conclusion. It seems to exist a consensus in fundamental and experimental studies on the detrimental effect of noise but results from epidemiological are not convincing and require further studies.
In vitro maturation of human oocytes and cumulus cells using a co-culture three-dimensional collagen gel system.

BACKGROUND Deficiencies remain in the ability of in vitro-matured human oocytes to acquire full developmental competence and give rise to a healthy pregnancy. A clear deficiency of current systems utilizing human oocytes has been the absence of cumulus cells. In the present study, a three-dimensional (3D) co-culture system exploiting an extracellular matrix was developed and compared to conventional methods for its ability to support maturation of human oocytes. METHODS AND RESULTS Cumulus cells were embedded into a 3D collagen gel matrix with individual oocytes added to each gel. Oocytes from the same patient cultured in the gel matrix matured to metaphase II at rates similar to those of cumulus-free oocytes cultured in individual microdrops. Following maturation of oocytes and fixation of intact gels, chromatin and cytoskeletal elements were assessed in oocytes and cumulus cells. The activities of the key cell cycle kinases, maturation-promoting factor (MPF) and mitogen-activated protein kinase (MAPK), were compared in oocytes matured under the two culture conditions. Compared with denuded oocytes, co-cultured oocytes exhibited increased MAPK activity, but no difference in MPF levels. CONCLUSIONS This work characterizes a novel and efficacious culture system that takes advantage of the unique properties of the extracellular matrix, a 3D microenvironment, and the presence of cumulus cells for maturing human oocytes in vitro.
The Relationship between Proliferating Cell Nuclear Antigen Expression and Histomorphometrical Alterations in Cyclosporin A-Induced Gingival Overgrowth in Rats

The aim of this study was to investigate the relationship between Proliferating Cell Nuclear Antigen (PCNA) expression and histomorphometrical alterations in cyclosporin A (CsA)-induced gingival overgrowth with or without microbial dental plaque accumulation. Forty male Wistar rats were equally divided into 4 groups; Group I (control); Group II (CsA); Group III (ligature); Group IV (ligature and CsA). After 8 weeks of experimental period, rats were subsequently decapitated and mandibular molars were dissected. Gingival overgrowth was determined by measuring depth of the gingival sulcus, then the mandible were decalcified and serial sections were obtained for histomorphometric and immunohistochemical analysis. Histomorphometric analysis included the measurement of epithelial thickness; immunohistochemical analysis included the assessment of PCNA expression in the oral and sulcular epithelium of buccal and lingual gingiva. Epithelial thickness and PCNA expression were significantly increased in buccal oral epithelium of Group II (p < 0.05) and in all regions in Group IV (p < 0.05) compared to control group. Also gingival overgrowth was more prominent in Group IV in comparison to Group II. These results indicate that CsA-induced gingival alterations are closely accociated with increased epithelial proliferative activity, and dental plaque accumulation seems not to be an essential but to be an aggrevating factor for the progression of the lesion.
Evaluación del rendimiento de redes IP utilizando plataformas de virtualización y métodos de simulación

Las plataformas de virtualizacion y los metodos de simulacion constituyen dos tecnologias prominentes en el ambito de la investigacion, que son utilizadas para medir el rendimiento de las redes IP. En esta investigacion se ha disenado, implementado y puesto en funcionamiento escenarios de red simulados (mediante NS-2) y virtualizados (mediante Xen) con el fin de validar el rendimiento de redes IP. Consecuentemente, se ha realizado varios experimentos utilizando estas dos tecnologias con el proposito de verificar como se va degradando el rendimiento de la red a medida que se incrementan equipos en la misma. Los resultados experimentales iniciales ilustraron que existen diferencias al evaluar el rendimiento de la red a pesar de someter las dos tecnologias con los mismos escenarios y a las mismas pruebas. Esto se debe en el caso de la Simulacion a que los parametros deben ser rigurosamente programados para mejorarlos. En cambio, en el caso de la Virtualizacion, se deben a la falta de adaptacion de otras condiciones operacionales como temporizacion y mejoramiento de la plataforma de hardware. En este contexto, se realizaron pruebas adicionales incluyendo diversos algoritmos de generacion de trafico, evaluacion del ancho de banda (BW) y estimacion de paquetes perdidos. Esto permitio finalmente determinar la degradacion del rendimiento de la red al evaluar los resultados obtenidos mediante Simulacion con NS-2 y Virtualizacion con Xen.