Miracle-dz/UniFAR-FTUs huggingface dataset:
The immunomodulatory effect of vitamin D in chickens is dose-dependent and influenced by calcium and phosphorus levels.

Vitamin D requirement is estimated to be higher than recommended values for the first two weeks of a broiler chicken's life, and is heavily dependent on the concentrations of Ca and P in the diet. There are data indicating the beneficial effect of higher vitamin D levels on performance and overall health of the chickens. However, data on the role of higher vitamin D levels on the innate immune response of chickens are limited. Therefore, in the current study, we examined the effect of higher doses of vitamin D supplementation on the innate immune response in broiler chickens receiving optimal or calcium (Ca) and phosphorus (P) deficient diets. Three hundred Ross-308 male broiler chicks were randomly allocated into 60 cages with 5 birds per cage in a 3 × 2 factorial design with three levels of vitamin D and two levels of Ca/P with each experimental diet fed to 10 cages (10 replicates). Quantitative reverse transcription PCR (n = 5) was used to assess Toll-like receptor (TLR2b and 4), cytokine/chemokine (IL-12, IFN-γ, IL-10, IL-4, IL-13, IL-18, CxCLi2) and cathelicidin (CATH1, CATHB1, CATH3) transcription levels in peripheral blood mononuclear cells (PBMCs), spleen, and bursa of Fabricius. Vitamin D supplementation of the Ca and P deficient diet considerably augmented transcription of TLR2b, TLR4, CATH1, and CATHB1 and predominantly Th2 cytokines in spleen. Supplementation of the control diet with vitamin D downregulated TLR4 transcription, and dose-dependently increased CATH1, CATHB1, Th1, and Th2 cytokine transcription (Th2>Th1). All diets downregulated CATH3 transcription. In conclusion, vitamin D or its derivative 25-OH-D3 both have a robust immunomodulatory property with a more favorable Th2 response, while at the same time enhancing observed Th2 cytokine responses under both optimal and lower Ca and P inclusion levels in the diets of broiler chickens.
Postcategorical Auditory Distraction in Short-Term Memory: Insights From Increased Task Load and Task Type

Task-irrelevant speech impairs short-term serial recall appreciably. On the interference-by-process account, the processing of physical (i.e., precategorical) changes in speech yields order cues that conflict with the serial-ordering process deployed to perform the serial recall task. In this view, the postcategorical properties (e.g., phonology, meaning) of speech play no role. The present study reassessed the implications of recent demonstrations of auditory postcategorical distraction in serial recall that have been taken as support for an alternative, attentional-diversion, account of the irrelevant speech effect. Focusing on the disruptive effect of emotionally valent compared with neutral words on serial recall, we show that the distracter-valence effect is eliminated under conditions—high task-encoding load—thought to shield against attentional diversion whereas the general effect of speech (neutral words compared with quiet) remains unaffected (Experiment 1). Furthermore, the distracter-valence effect generalizes to a task that does not require the processing of serial order—the missing-item task—whereas the effect of speech per se is attenuated in this task (Experiment 2). We conclude that postcategorical auditory distraction phenomena in serial short-term memory (STM) are incidental: they are observable in such a setting but, unlike the acoustically driven irrelevant speech effect, are not integral to it. As such, the findings support a duplex-mechanism account over a unitary view of auditory distraction.
A Method to Calculate Adherence to Inhaled Therapy that Reflects the Changes in Clinical Features of Asthma.

RATIONALE Currently, studies on adherence to inhaled medications report average adherence over time. This measure does not account for variations in the interval between doses, nor for errors in inhaler use. OBJECTIVES To investigate whether adherence calculated as a single area under the (concentration-time) curve (AUC) measure, incorporating the interval between doses and inhaler technique, was more reflective of patient outcomes than were current methods of assessing adherence. METHODS We attached a digital audio device (INhaler Compliance Assessment) to a dry powder inhaler. This recorded when the inhaler was used, and analysis of the audio data indicated if the inhaler had been used correctly. These aspects of inhaler use were combined to calculate adherence over time, as an AUC measure. Over a 3-month period, a cohort of patients with asthma was studied. Adherence to a twice-daily inhaler preventer therapy using this device and clinical measures were assessed. MEASUREMENTS AND MAIN RESULTS Recordings from 239 patients with severe asthma were analyzed. Average adherence that was based on the dose counter was 84.4%, whereas the ratio of expected to observed accumulated AUC, actual adherence, was 61.8% (P < 0.01). Of all the adherence measures, only adherence calculated as AUC reflected changes in asthma quality of life, β-agonist reliever use, and peak expiratory flow over the 3 months (P < 0.05 compared with other measures of adherence). CONCLUSIONS Adherence that incorporates the interval between doses and inhaler technique, and calculated as AUC, is more reflective of changes in quality of life and lung function than are the currently used measures of adherence. Clinical trial registered with www.clinicaltrials. gov (NCT 01529697).
Loudness summation of equal loud narrowband signals in normal-hearing and hearing-impaired listeners

Abstract Objective: Loudness perception of binaural broadband signals, e.g. speech shaped noise, shows large individual differences using frequency-dependent amplification which was adjusted to restore the loudness perception of monaural narrowband signals in hearing-impaired (HI) listeners. To better understand and quantify this highly individual effect, loudness perception of broadband stimuli consisting of a number of spectrally separated narrowband components which where individually adjusted to equal loudness is of interest. Design: Based on categorical loudness scaling, the loudness of an equal categorical loudness noise (ECLN) consisting of six third-octave noises was assessed. For loudness categories “medium” und “very loud” the required narrowband loudness was analysed. Study sample: Nine normal-hearing (NH) and ten HI listeners. Results: HI listeners showed lower narrowband loudness values compared to NH listeners, indicating an increased spectral loudness summation. More than 50% of the HI listeners showed higher binaural spectral loudness summation compared to NH listeners. The amount of binaural spectral loudness summation was highly correlated (r2 = 0.92) with the loudness level at “very loud” of aided speech shaped noise. Conclusions: The suggested ECLN measurement is suited to assess individual (binaural) broadband loudness in aided conditions, providing valuable information for hearing-aid fitting.
Three‐dimensional morphometric analysis of brain shape in nonsyndromic orofacial clefting

Previous studies report structural brain differences in individuals with nonsyndromic orofacial clefts (NSOFC) compared with healthy controls. These changes involve non‐uniform shifts in tissue volume within the cerebral cortex and cerebellum, suggesting that the shape of the brain may be altered in cleft‐affected individuals. To test this hypothesis, a landmark‐based morphometric approach was utilized to quantify and compare brain shape in a sample of 31 adult males with cleft lip with or without cleft palate (CL/P), 14 adult males with cleft palate only (CPO) and 41 matched healthy controls. Fifteen midline and surface landmarks were collected from MRI brain scans and the resulting 3D coordinates were subjected to statistical shape analysis. First, a geometric morphometric analysis was performed in three steps: Procrustes superimposition of raw landmark coordinates, omnibus testing for group difference in shape, followed by canonical variates analysis (CVA) of shape coordinates. Secondly, Euclidean distance matrix analysis (EDMA) was carried out on scaled inter‐landmark distances to identify localized shape differences throughout the brain. The geometric morphometric analysis revealed significant differences in brain shape among all three groups (P < 0.001). From CVA, the major brain shape changes associated with clefting included selective enlargement of the anterior cerebrum coupled with a relative reduction in posterior and/or inferior cerebral portions, changes in the medio‐lateral position of the cerebral poles, posterior displacement of the corpus callosum, and reorientation of the cerebellum. EDMA revealed largely similar brain shape changes. Thus, compared with controls, major brain shape differences were present in adult males with CL/P and CPO. These results both confirm and expand previous findings from traditional volumetric studies of the brain in clefting and provide further evidence that the neuroanatomical phenotype in individuals with NSOFC is a primary manifestation of the defect and not a secondarily acquired characteristic.
Beta‐catenin signaling in hepatic development and progenitors: Which way does the WNT blow?

The Wnt/β‐catenin pathway is an evolutionarily conserved signaling cascade that plays key roles in development and adult tissue homeostasis and is aberrantly activated in many tumors. Over a decade of work in mouse, chick, xenopus, and zebrafish models has uncovered multiple functions of this pathway in hepatic pathophysiology. Specifically, beta‐catenin, the central component of the canonical Wnt pathway, is implicated in the regulation of liver regeneration, development, and carcinogenesis. Wnt‐independent activation of beta‐catenin by receptor tyrosine kinases has also been observed in the liver. In liver development across various species, through regulation of cell proliferation, differentiation, and maturation, beta‐catenin directs foregut endoderm specification, hepatic specification of the foregut, and hepatic morphogenesis. Its role has also been defined in adult hepatic progenitors or oval cells especially in their expansion and differentiation. Thus, beta‐catenin undergoes tight temporal regulation to exhibit pleiotropic effects during hepatic development and in hepatic progenitor biology. Developmental Dynamics 240:486–500, 2011. © 2010 Wiley‐Liss, Inc.
Treatment of colored and real industrial effluents through electrocoagulation using solar energy

This study was undertaken to investigate the removal of Acid Orange 2 (sodium 4-[(2E)-2-(2-oxonaphthalen-1-ylidene) hydrazinyl] benzenesulfonate) and Reactive Blue 19 (2-Anthracenesulfonicacid,1-amino-9,10-dihydro-9,10-dioxo-4-[[3-[[2-(sulfooxy) ethyl] sulfonyl] phenyl] amino]-,sodium salt (1:2)) from synthesized and real effluents through electrocoagulation using solar cells for the purpose of improving economic efficiency of the process. The impact of a number of key operating parameters was explored including current density, anode type, temperature, pH, and electrolyte concentration. The current density of 45 Am−2 proved to be the optimum level for both dyes. The same optimum alternatives were found for the other parameters in both cases: iron anode, a temperature level of 25°C, a pH of 7, and an electrolyte concentration of 15 mg L−1. Both effluent samples were subjected to COD (chemical oxygen demand) and TOC (total organic carbon) tests. Cost analysis was performed for the treatment process.
Genetic analysis of the Pseudomonas aeruginosa PAO high-affinity branched-chain amino acid transport system by use of plasmids carrying the bra genes

About 30 mutants of Pseudomonas aeruginosa PAO defective in the high-affinity branched-chain amino acid transport system (LIV-I) were isolated by the selection for resistance to 4-aza-DL-leucine, a toxic leucine analog for LIV-I. All of the mutants were complemented by plasmid pKTH24, harboring the braC gene, which encodes the branched-chain amino acid-binding protein, and the four open reading frames named braD, braE, braF, and braG (T. Hoshino and K. Kose, J. Bacteriol. 172:5531-5539, 1990). We identified five cistrons corresponding to these bra genes by complementation analysis with various derivatives of pKTH24, confirming that the braD, braE, braF, and braG genes are required for the LIV-I transport system. We also found mutations that seem likely to be mutations in a promoter region for the bra genes and those with polarity in the intercistronic region between braC and braD. Analysis with an omega interposon showed that the bra genes are organized as an operon and are cotranscribed in the order braC-braD-braE-braF-braG from a promoter located in the 5'-flanking region of the braC gene.
Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis.

AIMS The aim of this study was to determine the occurrence and role of yeasts, enteric gram-negative rods and Enterococcus species in root-filled teeth with chronic apical periodontitis, and the antimicrobial effect of iodine potassium iodide (IKI) irrigation. METHODOLOGY Forty symptom-free root-filled teeth with chronic apical periodontitis were included in the study. The patients were divided into two groups. In group A the canals were filled with calcium hydroxide for 10-14 days after cleaning and shaping; in group B the canals were irrigated with IKI for 5 min after cleaning and shaping followed by a permanent root filling. Microbiological samples were taken from the canals before and after the chemomechanical preparation and after iodine irrigation (group B). RESULTS Microbes were isolated from 33 of 40 teeth in the initial sampling. Yeasts were isolated from six teeth, three of them together with E. faecalis. Enteric rods (Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis) were present in three teeth and E. faecalis was isolated from 21 of the 33 culture positive teeth, 11 in pure culture. Growth was detected in 10 teeth of the second samples. Six of the 10 cases were E. faecalis, with five being a pure culture. All third samples (after IKI) except one were negative. The number of microbial cells per sample did not correlate with lesion size. Two flare-ups were recorded, both in teeth with a mixed infection. CONCLUSION The high prevalence of enteric bacteria and yeasts in root-filled teeth with chronic apical periodontitis was established. IKI improved the antimicrobial effect of the treatment.
Optimal Local Approximation Spaces for Generalized Finite Element Methods with Application to Multiscale Problems

The paper addresses a numerical method for solving second order elliptic partial differential equations that describe fields inside heterogeneous media. The scope is general and treats the case of rough coefficients, i.e., coefficients with values in $L^{\infty}(\Omega)$. This class of coefficients includes as examples media with microstructure as well as media with multiple nonseparated length scales. The approach taken here is based on the the generalized finite element method (GFEM) introduced in [I. Babuska, G. Caloz, and J. E. Osborn, SIAM J. Numer. Anal. , 31 (1994), pp. 945–981] and elaborated in [I. Babuska, U. Banerjee, and J. Osborn, Int. J. Comput. Methods, 1 (2004), pp. 67–103], [I. Babuska and J. Melenk, Internat. J. Numer. Methods Engrg. , 40 (1997), pp. 727–758], and [T. Strouboulis, I. Babuska, and K. Copps, Comput. Methods Appl. Mech. Engrg. , 181 (2001), pp. 43–69]. The GFEM is constructed by partitioning the computational domain $\Omega$ into a collection of preselected subsets $\omega_{i}.
The development of hunger and fullness during a laboratory meal in patients with binge eating disorder.

OBJECTIVE This study aimed to test the hypothesis that, compared to similarly obese participants without BED, individuals with BED have a disturbance in the development of fullness and reduction of hunger during the course of a standard meal of large size. METHOD Thirteen patients with BED and 14 obese control participants consumed 975 g of a milkshake. Participants received no information about how much they had eaten or how much of the meal remained to be consumed. Participants were interrupted after every 75 g consumed to rate hunger and fullness. RESULTS Final fullness ratings were higher in patients with BED, but there were no differences in mean duration or mean rate of eating, or in changes in subjective ratings of hunger and fullness per gram of food. DISCUSSION The current study reports the surprising finding of no difference in reports of hunger and fullness between patients with BED and obese controls.
The AP-1 transcription factor component Fosl2 potentiates the rate of myocardial differentiation from the zebrafish second heart field

The vertebrate heart forms through successive phases of cardiomyocyte differentiation. Initially, cardiomyocytes derived from first heart field (FHF) progenitors assemble the linear heart tube. Thereafter, second heart field (SHF) progenitors differentiate into cardiomyocytes that are accreted to the poles of the heart tube over a well-defined developmental window. Although heart tube elongation deficiencies lead to life-threatening congenital heart defects, the variables controlling the initiation, rate and duration of myocardial accretion remain obscure. Here, we demonstrate that the AP-1 transcription factor, Fos-like antigen 2 (Fosl2), potentiates the rate of myocardial accretion from the zebrafish SHF. fosl2 mutants initiate accretion appropriately, but cardiomyocyte production is sluggish, resulting in a ventricular deficit coupled with an accumulation of SHF progenitors. Surprisingly, mutant embryos eventually correct the myocardial deficit by extending the accretion window. Overexpression of Fosl2 also compromises production of SHF-derived ventricular cardiomyocytes, a phenotype that is consistent with precocious depletion of the progenitor pool. Our data implicate Fosl2 in promoting the progenitor to cardiomyocyte transition and uncover the existence of regulatory mechanisms to ensure appropriate SHF-mediated cardiomyocyte contribution irrespective of embryonic stage. Summary: Early embryonic heart muscle differentiates in phases from two progenitor populations. AP-1 promotes the progenitor-to-cardiomyocyte transition specifically during the second phase.
The Correlation between Value Added Tax and Economic Growth in Romania

The study aims to investigate the relationship between the revenues collected from the VAT and the GDP in Romania, for the period of 1993-2016. The interest in studying this relationship derives from the importance gained by this tax over the years and from the actuality of the economic growth in Romania. The methods used are: estimating a regression model and estimating the autoregressive vector model using Eviews 9. The regression model has the gross domestic as a dependent variable and as an independent variable the revenues from the VAT. The results obtained from the analysis indicates that there is a positive and direct relationship between the two variables and the variation of the GDP is explained by the revenues from the VAT
Distribution of Ultrabithorax proteins in Drosophila

We have used a monoclonal antibody to examine the distribution of Ultrabithorax (Ubx) proteins in Drosophila embryos and imaginal discs by immunofluorescence. Ubx proteins are nuclear and show a spatially restricted distribution in the nervous system, epidermis and mesoderm. Labelling extends from the first thoracic segment (T1) to the eighth abdominal segment (A8) in the midline cells, from T2 to A8 in the ventral nervous system and epidermis and from A1 to A8 in the somatic mesoderm. In the nervous systems and epidermis the patterns of labelling exhibit a repeat unit, the Ubx metamere, that is out of phase with the segmental repeat unit. At least in the epidermis this repeat unit appears to extend between anterior‐posterior compartment boundaries and consists of a posterior compartment together with the succeeding anterior compartment. The most prominently labelled metamere in the nervous system and epidermis is that comprising the posterior region of T3 and the anterior region of A1. Within each metamere the nuclei are heterogeneously labelled. Clear heterogeneity of labelling is also seen amongst the nuclei of the T3 imaginal discs.
Alphabet pattern approach for color fabric texture classification

The present paper proposes a new approach for color fabric texture classification based on the occurrences of alphabet patterns. Generally, Patterns are identified on grey level image but the present approach identifies the patterns on RGB color image. The present approach identify the patterns on 3 color channel i.e. Red channel, Green channel and Blue channel. The present approach uses alphabet patterns using 5 pixels in 3×3 sub image. Based on the cumulative occurrences of 5 pixel alphabet patterns on 3×3 sub image, the present paper derives a user defined algorithm to classify the fabric textures into one of the four categories i.e. Silk, Woolen, Nylon and Cotton.
Acceptability of universal screening for intimate partner violence in voluntary HIV testing and counseling services in South Africa and service implications

Abstract Women who experience intimate partner violence (IPV) are more likely to be HIV positive. Many of these women never receive any relevant services, and those who do often access them only after events have escalated to a crisis requiring the police or emergency medical care. Evidence from other settings suggests that routinely asking women about experiences of partner violence is received positively. Voluntary counseling and testing (VCT) services would seem an ideal opportunity to screen for IPV in South Africa. However, in low resource settings, VCT is carried out by lay counselors with few skills. We therefore conducted a qualitative study that explored women's experience of IPV screening in VCT services, and explored implications for VCT counseling. The study was conducted in a clinic in Johannesburg where we trained the lay counselors to do IPV screening. In-depth interviews were conducted with 35 women attending VCT services. Participants were recruited before they attended VCT, and 12 women participated in a follow-up interview. A focus group was held with lay counselors a year after the intervention was implemented. Findings suggest that women were supportive of being asked about their experiences of IPV during VCT sessions. Reasons for supporting IPV screening at VCT services include the limited access of many women to health services. Many women who were aware of their HIV risk felt powerless to discuss condom use, HIV testing, and infidelity with their male partners. Women directly related such experience of gender power inequality to HIV risk. One year after training, there was no evidence of screening continuing. Our findings suggest that the focus should shift from asking about violence to equipping counselors to discuss gender inequality in relationships more broadly. This needs to be part of basic counselor training as it is integral to approaching HIV risk reduction.