Miracle-dz/UniFAR-FTUs huggingface dataset:
Dissecting and Modeling Oncogene Dependent Molecular Mechanisms in Lymphoma Genesis and Progression

Aggressive Non-Hodgkin lymphomas (aNHL) are a heterogeneous group of lymphomas. The constellation of oncogenic pathway activities can vary in patients with the same malignant disease. The pathological mechanisms behind the current criteria to distinguish individual aNHL subtypes are still poorly understood. Molecular models depicting the dynamics of oncogenic signaling and cross talk of the pathways can help to obtain a better understanding of lymphoma pathogenesis. Thereby the development of future targeted therapies, that will have to intervene with specific signaling modules to reverse pathway deregulation, will be enabled. We postulate that the description of a time-resolved global gene expression change provides new insights into the dynamics of oncogenic signaling and that the intervention into pathway intersections allows identifying new essential pathway nodes and cross talks for targeted therapies. To gain closer insight into distinct cell signaling networks and their relevance for NHL subtypes, firstly, high-dimensional data of time-resolved gene expression changes in BL2 cells induced by BCR or CD40 activation were generated. By using a newly established computational method we were able to describe 20 disjoint sets of genes to group their time courses. 15 significant sets of genes were identified for BCR stimulated samples and 5 significant sets of genes for CD40 activated samples, all displaying distinct time courses. We discovered a co-repression of LEF1, PTCH1 and NOTCH1 by activation of the B cell receptor. Functional assays revealed that BL cells are not responsive to Sonic hedgehog stimulation, but evidence is provided for a c-Myc-driven non-canonical regulation of this pathway. Furthermore, a working model for a causality network of BCR induced gene expression correlations was compiled. In addition, sustained activated BCR signaling induced a change of the gene expression profile towards a certain ‘DLBCL-likeness’. Therefore, a first step to develop a model displaying the causative factors for the non-mBL signature is provided. Secondly, the gene expression changes of BCR or CD40L stimulated BL2 cells after chemical inhibition of Tak1, JNK, p38 and IKK2 were monitored on the whole genome level. In BCR stimulated cells the activity of PI3K and Mek1/2 was as well inhibited. Additional biochemical analyses supported the view that Tak1 is a pivotal modulator of both CD40 and BCR mediated p38 and NF-κB signaling. Moreover, using (5Z)-7-Oxozeanol as well as TAK1-directed siRNA to inhibit Tak1 activity, a corresponding Tak1-Erk1/2 subnetwork was described. Thus, the present study and the herein allocated data provide deep insight into oncogenic pathway activities and enable a large variety of continuing studies, which may help to obtain a better understanding of the biology of lymphoma.
Epigenetic changes involved in hydroquinone-induced mutations

Abstract Hydroquinone (HQ), an active metabolite of benzene, plays an important role in the malignant hematopathy caused by benzene. Malignant transformation has been observed after 20 or 29 weeks of in vitro exposure to HQ and two years of in vivo exposure to HQ. Many epigenetic events have been identified in the process of HQ-induced mutations, suggesting that epigenetic changes may play important roles in the onset and process of HQ-induced carcinogenesis. In this review, we for the first time summarize the epigenetic mechanisms identified following exposure to HQ to provide more potential biomarkers and targets for the diagnosis and treatment of malignant hematopathy induced by benzene and its metabolites. The identified epigenetic changes are as follows: global DNA hypomethylation; hypomethylation of the thrombopoietin receptor MPL proto-oncogene; hypermethylation of the Rb and PTEN tumor suppressor genes; hypermethylation of erythroid-specific genes; enzymology of DNA methylation; phosphorylation, acetylation, and methylation of histones; aberrant expression of miRNAs and lncRNAs; and chromatin remodeling. Graphical Abstract
Lesion frequency in external genitals of bottlenose dolphins Tursiops truncatus in the north-central keys of Cuba.

Among the most recent described viral diseases for bottlenose dolphins Tursiops truncatus is Papillomatosis; a kindly tumour entity that affects skin and mucosa of different parts in the body of aquatic and terrestrial animals, including humans. With the goal to determine the presentation frequency of these lesions in external genital, 263 bottlenose dolphins from north central coast of Sabana-Camaguey archipelago were studied between the years 2000 and 2010. Based on clinical identification of external warty lesions compatible with Papilloma, the 30% of all animals presented them. The presentation frequency of these lesions in males was higher than in females (20% vs. 10%, p<0,001). Besides, it was evident that immature animals are more susceptible to the disease than adults (25% vs. 5%). This is the first report in Cuban waters and the second in Atlantic waters, about the presence of Papilloma in genital of wildlife bottlenose dolphins, with a high and sustained presentation frequency. (
Etude des mécanismes physiologiques et moléculaires permettant la prise en charge des substrats hydrophobes par la levure Yarrowia lipolytica au niveau pariétal

Yarrowia lipolytica a la particularite de pousser sur de nombreux substrats hydrophobes et de les metaboliser via la β-oxydation ou de les stocker dans des corps lipidiques. Le premier contact entre la cellule et les substrats hydrophobes se deroule sur la surface cellulaire. A cette etape, la composition de la paroi cellulaire en proteines, en β-glucane et en chitine pourrait jouer un role important sur l’adhesion et la prise en charge des substrats hydrophobes. Dans le cadre de cette these, nous avons etudie l’effet d’une source de carbone hydrophobe (par rapport au glucose) et de la composition du milieu de culture sur la structure parietale et la production de melanine chez Y. lipolytica. Les resultats de nos analyses biochimiques et moleculaires ont montre que l’oleate de methyle (un compose utilise comme modele de source de carbone hydrophobe) a induit des modifications au niveau de la paroi cellulaire et que ces modifications ont conduit ou non a l’adhesion de gouttelettes lipidiques a la surface cellulaire ; a une modification du contenu des composes parietaux ; a la resistance des cellules a des composes toxiques ayant pour cible la paroi cellulaire ; et a la modification des profils d’expression de 27 genes analyses codant des proteines de paroi cellulaire. L’identite des genes surexprimes suggere la participation de leurs proteines dans l’integrite de la paroi cellulaire, dans l’adhesion des gouttelettes lipidiques et dans la reponse au stress par l’oleate de methyle et/ou le manque d’azote. Nos resultats ont aussi montre que la presence d’oleate de methyle, huile de ricin et ricinoleate de methyle a induit la production de DHN-melanine chez une souche genetiquement modifiee de Y. lipolytica (MTLY40-2p) dont la β-oxydation est affectee
Individual-based population assessment for cetaceans : using photographs to infer abundance, demography and individual quality

Seasonal reproduction in odontocetes has primarily been linked to bottom-up forcing through changes in the physical environment and/or prey availability, with little consideration of top-down selection through seasonal changes in predation pressure. Here we report on a distinct seasonal peak in calving for bottlenose dolphins (Tursiops truncatus) using Little Bahama Bank, despite its tropical latitude, and describe an inverse seasonal peak in the risk of predation by sharks, inferred by the incidence of fresh shark-bite wounds. Analysis of photo-identification data from 1992-2007 revealed that all (16/16) neonates were documented during a six month “winter” period (Nov-Apr), whereas 13/16 of all fresh shark-bite wounds were documented during the contrasting six month “summer” period (May-Oct). Notably, the timing of wound acquisitions coincided with the season for intense tropical cyclones in the NW Atlantic, and 11/16 of the fresh wounds were documented immediately after three intense hurricanes passed directly over the study site, likely forcing dolphins into deeper waters with higher risk of predation by sharks. We suggest that calving seasonality may be at least in part a response to the selective disadvantage of rearing a young calf during the potentially dangerous summer. Predation and calving seasonality in Tursiops CHAPTER 2
LIVING WITH HIGH BURDEN OF CARE: EXPERIENCE OF CAREGIVERS OF PEOPLE WITH SEVERE MENTAL ILLNESS IN RURAL ETHIOPIA

Aims/Purpose: Caregivers of people with severe mental illness (SMI) experience substantial burden from providing care. This burden is likely to be higher in low- and middle-income countries where statutory medical and social services are scarce. We conducted a study aiming to evaluate the level of caregiver burden among caregivers of people with SMI. Methods: The study was population-based cross-sectional evaluation conducted in the Sodo district of the Gurage zone in south Ethiopia. Three hundred people with SMI and matched caregivers were included. The involvement evaluation questionnaire (IEQ), a widely used tool for assessing caregiver burden, was administered. The core tool assessing burden has four domains (urging, worry, supervision and tension). Results: Caregivers were predominantly women (52.5%), married (67.6%), under 45 years of age (57.5%) and had little or no formal education (60.9%). The overall mean burden score (95% confidence interval (CI)) was 45.3 (43.2, 47.4); highest for “urging” (mean=15.5; 95% CI=14.6, 16.4) and “worrying” domains (mean=13.7; 95% CI=12.9, 14.4). Being female caregiver, first degree relative, experiencing distress and caring for patients with higher levels of symptomatology and disability were significantly associated with higher overall burden score. Conclusions: As anticipated, caregivers in this setting have substantially higher levels of burden of caring compared with care givers in Europe and other settings. Interventions should aim not only at decreasing patient symptoms and improving functionality, but also at decreasing caregiver distress focused on women and first-degree relatives.
Effect of HRM Practices on Constructive Deviance in Pharmaceuticals Companies: Mediating by Ethical Climate

The purpose of the current study is to elaborate that how ability, motivation and opportunity enhancing human resource management practices effect on constructive deviance in pharmaceuticals companies. The study also examine that how ethical climate mediates the relationship between them. In present study the data is collected from pharmaceuticals companys employees of Thailand. Under this study, the AMO theory is essential to explain the importance of the research framework. It is considered as fundamental theory and this study add knowledge in ability, motivation and opportunity theory. For fulfilling the nucleus aim of the current study the deductive approach is used. The data is collected from employees through survey method. The questionnaire method is used under this study for collecting the data from the respondents. Simple random sampling technique is used under this study for data collection from 300 employees. Total 300 questionnaires are distributed out of which 270 questionnaire collected from employees. The smart PLS is used under this study for analyzing the relation among variables. The study is correlational among variables. The primary concern of this study is to identifying the relationship among variables such as AMO enhancing HRM practices, constructive deviance and ethical climate. Moreover, there are limited studies available on pharmaceuticals human resource management department of Asian economies. Still, the human resource management practices are not properly followed in developing countries. So, this study provides greater knowledge about HRM practices according to ability, motivation and opportunity theory. Similarly, the practical implication and future direction are provided at the end of the study under the discussion heading.
Smartphone Addiction andndash; A New Disorder or Just a Hype

For last few years there has been a considerable interest in the field of Behavioral addiction. As a result of which Pathological gambling has been included in DSM-V as a disease entity. With the advent of smartphone and its huge popularity among youngsters, has raised the issue of Smartphone addiction. We searched the internet with the key words – smartphone addiction, adolescents, practical solution, digital literacy and behavioral addiction. From the selected studies we analyzed the-risk factors, assessment and diagnosis, consequences and practical solutions for the smartphone addiction in youngsters.
METHOD FOR ASSESSING THE STRUCTURAL RELIABILITY OF NETWORKS WITH UNDETERMINED TOPOLOGY

This paper shows the relevance of the task of assessing the structural reliability of networks with undetermined topology. Proposed is a method for assessing the structural reliability of networks of undetermined topology based on taking into account the basic structural characteristics of the network (the number of nodes and branches, the degree of network connectivity, the maximum allowable rank of paths, and others). To obtain an estimate of the structural reliability for a network of any dimension and any topology, expressions are proposed in the scientific research to determine the number of paths of various ranks, which must be taken into account when calculating the structural reliability index by the upper and lower bounds method.
Application of Microsatellite Markers in Grapevine and Olives

Since their discovery in the 80s, microsatellites have become a popular molecular marker for 9 studying plant genomes and are still the marker system of choice for various applications, 10 such as genetic diversity and genetic structure studies, fingerprinting of individuals, parent‐ 11 age analyses and mapping studies. Although they have been used as a PCR marker system 12 for more than 20 years now [1, 2], the numerous recent publications on their use confirm 13 their durability and relevance. This is mainly due to their intrinsic properties (associated 14 high polymorphisms) and a constant evolution of the technical methodology in terms of 15 high throughput, ease of use and price. The starting methodology was based on radioactive 16 labelled amplified microsatellite alleles separated on polyacrilamide gels. Nowadays, highly 17 multiplexed fluorescently labelled microsatellites are commonly genotyped in capillary 18 based automatic systems. 19
Structure and activity investigations of the cell fate determinant, SpoIIE, from Bacillus subtilis

For many years the Gram positive bacterium Bacillus subtilis has been a model organism for prokaryotic cell and molecular biology. The asymmetric cell division which B. subtilis undergoes during sporulation is a simple system by which to study the process of cell differentiation. Sporulation is governed by a series of genetic temporal and spatial controls. Gene regulation brought about by a series of σ factors and transcriptional regulators is coupled to key morphological stages or checkpoints. σF initiates the first step in a cascade of complex genetic control which eventually produces a resilient endospore. The activation of σF, the first compartment-specific sigma factor, in the forespore and its regulation through interaction between three proteins; SpoIIAA, SpoIIAB and SpoIIE, is of particular interest. SpoIIE, a protein phosphatase which binds to the asymmetric division septum, is a crucial factor in the selective activation of σF in the forespore. Of three putative domains in SpoIIE only the C-terminal PP2C phosphatase domain has been structurally characterised. The central domain, domain II, of SpoIIE has been assigned a role in interaction with the cell division machinery; however mutational studies have shown that, in addition, this domain is also responsible for the regulation of phosphatase activity. This work describes the isolation and characterisation of three new fragments of SpoIIE containing elements of the central cytoplasmic domain of SpoIIE. These include a fragment found to accurately represent the N-terminal solubility limit of domain II which shows a high degree of oligomeric character. The fragments isolated show specific phosphatase activity against SpoIIAA~P, albeit at reduced rates compared to the free phosphatase domain, which indicates an inhibitory role for SpoIIE domain II against the PP2C domain. Three ultimately unsuccessful approaches were attempted to isolate a co-complex of SpoIIE and SpoIIAA~P for structural characterisation. A tendency for domain II containing SpoIIE fragments to precipitate in the presence of Mn(2+) is also identified. An in vivo investigation into the sporulation efficiencies of amino acid substitutions in a potential regulatory interface between domains II and III of SpoIIE indicated no strong sporulation defects.
Artificial Neural Network for Accurate Retrieval of Fiber Brillouin Frequency Shift With Non-Local Effects

Brillouin optical time-domain analysis (BOTDA) that operates over a long sensing fiber is prone to be affected by the detrimental non-local effects (NLE); since NLE can distort Brillouin gain spectrum (BGS), therefore correctly retrieving Brillouin frequency shift (BFS) is very challenging. Recently, the basic artificial neural networks (B-ANN) has been demonstrated to retrieve BFS effectively, in the case that a distortion-free BGS can be obtained. However, in the more general cases with NLE, a neural network for retrieving BFS in BOTDA data has not been proposed. In this paper, firstly the physical origin of NLE is analyzed theoretically and experimentally in detail. Then a specific ANN (NLE-ANN) is proposed to deal with the BOTDA data affected by NLE for the first time. The experimental verifications show that with the cooperative implementation of NLE-ANN and B-ANN, the effective retrieval of BFS along the whole fiber can be achieved, even though the BGS has been affected by NLE. This work proposes a promising approach in traditional BOTDA, because the upper limit of probe power could be raised by artificial intelligence to boost the sensing performance.
Efficient Pseudo-chaotic Number Generator for Cryptographic Applications

A secure and efficient pseudo-random number generator is crucial for the security of a stream cipher or block cipher in a cryptosystem. This paper is devoted to the design and analysis of a new robust Pseudo-Chaotic Number Generator (PCNG) based on discrete chaotic maps over a finite field, and a multiplexing mechanism. The proposed PCNG contains three discrete chaotic maps: Piece-Wise Linear Chaotic Map (PWLCM), skew tent and logistic map. XOR operators are applied on these maps to form three optional intermediate outputs that are selected by the multiplexing mechanism to produce the final pseudo-chaotic sequence. The XOR operators and multiplexing mechanism can increase the dynamic pseudo-chaotic properties significantly. The PCNG is designed over a finite 2 32 field, has a simple structure and can be easily implemented. It has been implemented on a new stream cipher. Security analyses and statistical experiments have been carried out and the results have proven that the proposed PCNG can generate effective pseudo-random numbers and the stream cipher exhibits good security properties with large secret key space and high key sensitivity. Therefore, the proposed PCNG can be successfully applied in cryptosystems.
Radiation Dose from Computed Tomography Scanning in Patients at Songklanagarind Hospital: Diagnostic Reference Levels

Objective: To determine diagnostic reference levels (DRLs) of computed tomography (CT) radiation doses in terms of CT dose index volume (CTDIvol) and dose length product (DLP) of CT scans of the head, chest and abdomen for patients at Songklanagarind Hospital, Thailand. Material and Methods: This was a retrospective analysis of 463 randomly selected head, chest and abdominal CT stuides from 416 patients enrolled from July 1st to 31st 2017. The CTDIvol, DLP and clinical indication for each CT study were conducted. The median and third quartile values were analysed and compared to the standard international DRLs. The DRL was defined as the third quartile value. Results: The DRLs for CTDIvol, and DLP of head, chest and whole abdominal CT were 57.5, 11.6 and 13.1 milliGray (mGy), and 1,102.6, 474.7 and 624.4 milliGray x centimetre (mGy.cm), respectively. The most common clinical indications were stroke (29.1%) for head CT and malignancy for both chest (73.6%) and abdominal CTs (49.6%). Conclusion: The DRLs of each CT region were mostly below standard international DRLs of Australia, Europe, Japan, the United Kingdom and the United States. The clinical indication for malignancy had significant difference in the DLP values than other clinical indications in head and chest CT.
Detecção Automática de Descargas Epileptiformes Interictais com o Uso de Redes Complexas

A epilepsia e uma desordem cerebral caracterizada pela presenca de convulsoes que afeta em torno de 1% de toda a populacao mundial. Convulsoes sao episodios que podem ser breves e quase imperceptiveis ou podem ser longos e dolorosos (convulsoes tonico-clonicas generalizadas) [3]. O eletroencefalograma (EEG) e um dos testes diagnosticos mais importantes para a investigacao de pacientes epilepticos com disturbios convulsivos. Atraves da analise do sinal de EEG e possivel identificar padroes patologicos de atividade entre convulsoes conhecidas como Descargas Epileptiformes Interictais (DEIs). As DEIs sao claramente diferenciadas da propria crise epileptica e sua identificacao pode auxiliar o prognostico de crises epilepticas [2]. [. ]
Consumer ethics: insights from business professionals

This research aims to generate new insights into consumer ethics by tapping into business executives' first-hand experience. The overarching goal of this novel, discovery-oriented approach is to illuminate the interactive relationships between business and consumer ethics, and to offer contextualized insights into consumers' (un)ethical behaviors. ,Three focus group interviews were conducted with senior business executives representing nine different industry sectors. Thematic analysis was performed to identify key themes for an integrative model. ,Four key themes emerged, highlighting: (1) the mutual influence between business and consumer ethics, (2) the nature and intensity of consumer ethics, (3) the dual influence of digital communication, and (4) the partial influence of consumer education. The themes gave rise to an integrative conceptual model. ,This research was limited somewhat by the small and judgmental sample. ,Consumers' growing demands for business ethics underscore the need for companies to elevate ethical considerations. The amplified consumer voice on social media is dreaded by business practitioners and is regarded as unethical consumer behavior to be actively managed. ,Business and consumer ethics can mutually influence each other in a benign or vicious circle. Consumer education is effective in some but not all domains. ,Business practitioners' insights reveal (1) the interactivity of business and consumer ethics and (2) the diversity of (un)ethical consumer behaviors. They point to the need for an enriched definition of consumer ethics and an expansion on the categorical structure of consumers' (un)ethical practices.