Miracle-dz/UniFAR-FTUs huggingface dataset:
SOCS1 methylation in patients with newly diagnosed acute myeloid leukemia

The proliferation and differentiation of hematopoietic precursor cells depend on various cytokines. The suppressor of cytokine signaling‐1 (SOCS1) down‐regulates Janus kinases/signal transducers and activators of transcription (JAK/STAT) pathway activity and inhibits the biological effects of cytokines. SOCS1 has been shown to have tumor‐suppressor activity, and methylation of this gene, resulting in transcriptional silencing, has been found in 65% of hepatocellular carcinoma and has been suggested to play an important role in the development of the cancer. The methylation status of the SOCS1 gene in acute myeloid leukemia (AML) has not been reported before. In this study, we analyzed SOCS1 methylation in 89 patients with newly diagnosed AML and correlated the result with immunophenotypes, cytogenetics, clinical features, and treatment outcome. SOCS1 methylation was found in the leukemic cells from 53 patients (60%). Thirteen (76%) of the 17 patients with t(15;17) had SOCS1 methylation, whereas this gene was methylated in only one (11%) of the nine patients with t(8;21). The frequencies of SOCS1 methylation among various cytogenetic subgroups differed significantly (P = 0.014). Other clinical and laboratory parameters and the disease‐free survival and overall survival were similar between patients with and without SOCS1 methylation. In conclusion, SOCS1 methylation occurs in more than half of AML cases, correlates with cytogenetic abnormalities, and may play an important role in the development of subsets of AML. © 2003 Wiley‐Liss, Inc.
ACE ID genotype and the muscle strength and size response to unilateral resistance training.

PURPOSE To examine associations among the angiotensin I-converting enzyme (ACE) insertion (I)/deletion (D) polymorphism and the response to a 12-wk (2 d.wk) unilateral, upper-arm resistance training (RT) program in the trained (T, nondominant) and untrained (UT, dominant) arms. METHODS Subjects were 631 (mean+/-SEM, 24.2+/-0.2 yr) white (80%) men (42%) and women (58%). The ACE ID genotype was in Hardy-Weinberg equilibrium with frequencies of 23.1, 46.1, and 30.8% for ACE II, ID, and DD, respectively (chi=1.688, P=0.430). Maximum voluntary contraction (MVC) and one-repetition maximum (1RM) assessed peak elbow flexor muscle strength. Magnetic resonance imaging measured biceps muscle cross-sectional area (CSA). Multiple variable and repeated-measures ANCOVA tested whether muscle strength and size differed at baseline and pre- to post-RT among T and UT and ACE ID genotype. RESULTS Baseline muscle strength and size were greater in UT than T (P<0.001) and did not differ among ACE ID genotype in either arm (P >or= 0.05). In T, MVC increases were greater for ACE II/ID (22%) than DD (17%) (P<0.05), whereas 1RM (51%) and CSA (19%) gains were not different among ACE ID genotype pre- to post-RT (P >or= 0.05). In UT, MVC increased among ACE II/ID (7%) (P<0.001) but was similar among ACE DD (2%) pre- to post-RT (P >or= 0.05). In UT, 1RM (11%) and CSA (2%) increases were greater for ACE DD/ID than ACE II (1RM, 7%; CSA, -0.1%) (P<0.05). ACE ID genotype explained approximately 1% of the MVC response to RT in T and approximately 2% of MVC, 2% of 1RM, and 4% of CSA response in UT (P<0.05). CONCLUSION ACE ID genotype is associated with the contralateral effects of unilateral RT, perhaps more so than with the muscle strength and size adaptations that result from RT.
Structured Interviews and the Diagnosis of Personality Disorders*

Structured interviews were developed to improve the reliability of the diagnosis of personality disorders, as it has occurred in other areas of psychopathology. Several such interviews are described in this review, which draws together reports scattered across a wide literature. The appearance of structured interviews in Britain reflected the early interest there in the special problems associated with diagnosing personality disorders, whereas in America their development had to await publication of the DSM-III. Studies using these techniques have illustrated important aspects of clinical diagnosis and can be expected to assign value to the diagnosis of personality disorders in the future.
Neuroendocrine differentiation in colorectal carcinomas.

OBJECTIVE To evaluate neuroendocrine differentiation in tumours from patients with colorectal carcinomas by immunostaining with antibodies against human chromogranin A (CgA) and neuron-specific enolase (NSE), and to correlate these finding with serum levels of CgA and pancreastatin-like immunoreactivity (PST-LI). We also investigated the degree of neuroendocrine differentiation found in colorectal carcinomas of different embryonic origins (hindgut and midgut). PATIENTS AND METHODS The presence of CgA and NSE in tumours from 91 patients with colorectal carcinoma was investigated using immunohistochemistry; levels of CgA and PST-LI in sera from 55 of these patients were determined using radioimmunoassays. RESULTS Immunostaining for CgA and NSE was found in 15 and 36% of tumours, respectively. One or both markers of neuroendocrine differentiation were demonstrated in 40% of the colorectal carcinomas. In patients who died during the study, 23 and 51% expressed CgA and NSE in their tumours, respectively, while the corresponding values in survivors were 11 and 27% (P = 0.14 and P = 0.025, respectively). The median survival time tended to be lower in patients with neuroendocrine differentiation of their tumours than in those without such differentiation (P = 0.1). The expression of NSE was significantly higher in colorectal carcinomas derived from the midgut than in those of hindgut origin. Elevated serum levels of CgA and PST-LI were found in 38 and 43% of the patients, respectively; 62% had elevated levels of one or both markers. Of the patients with elevated serum levels of CgA or PST-LI, 44% had positive immunohistochemistry for either NSE or CgA compared with 29% of those with normal serum levels of CgA and PST-LI (P = 0.27). Serum levels of CgA were increased in a significantly higher percentage of patients with colorectal carcinomas of midgut than of hindgut origin (63 and 28%, respectively, P = 0.03). Of patients with hindgut-derived carcinomas who died during the study, 64% had raised serum values of CgA compared with 19% of those who survived (P = 0.023). The corresponding figures for PST-LI elevation were 75 and 44%, respectively (P = 0.096). In the midgut group, no difference was demonstrated for these parameters between survivors and non-survivors. CONCLUSION Neuroendocrine differentiation was demonstrated in 40% of the colorectal carcinomas investigated and was more frequent in midgut-derived carcinomas than in those of hindgut origin. We have shown for the first time that serum levels of CgA and PST-LI are elevated in patients with colorectal carcinomas. In accordance with previous studies, our data support the value of neuroendocrine differentiation as an indicator of poor prognosis; they also suggest a role for serum CgA and PST-LI as prognostic markers.
Decreased platelet expression of myosin regulatory light chain polypeptide (MYL9) and other genes with platelet dysfunction and CBFA2/RUNX1 mutation: insights from platelet expression profiling

Summary. We have reported on a patient with thrombocytopenia, impaired platelet aggregation, secretion, phosphorylation of pleckstrin and myosin light chain (MLC), and GPIIb–IIIa activation, associated with a heterozygous mutation in transcription factor CBFA2 (core binding factor A2, RUNX1 or AML1). To obtain insights into the abnormal platelet mechanisms and CBFA2‐regulated genes, we performed platelet expression profiling in four control subjects and the patient using the Affymetrix U133 GeneChips. In the patient, 298 probe sets were significantly downregulated at least 2‐fold. MLC regulatory polypeptide (MYL9 gene) was decreased ∼77‐fold; this is an important finding because agonist‐stimulated MLC phosphorylation is decreased in patient platelets. Genes downregulated ≥ 5‐fold include those involving calcium binding proteins (CABP5), ion transport (sodium/potassium/Ca exchanger, SLC24A3), cytoskeletal/microtubule proteins (erythrocyte membrane protein band 4.1‐like 3, EPB41L3; tropomyosin 1, TPM1; tubulin, alpha 1, TUBA1), signaling proteins (RAB GTPase activating protein 1‐like, RABGAP1L; β3‐endonexin, ITGB3 BP) and chemokines (platelet factor 4 variant 1, PF4V1; chemokine CXCL5, CXCL5). These and other downregulated genes are relevant to the patient's platelet defects in function and production. These studies provide the first proof of concept that platelet expression profiling can be applied to obtain insights into the molecular basis of inherited platelet defects.
Los eventos, herramienta clave en las estrategias de comunicación de marcas turísticas: de la linealidad a la transmedialidad

espanolLa presente investigacion aborda el reto que plantea la utilizacion de eventos en la creacion y desarrollo de marcas turisticas. Desde el enfoque del protagonismo absoluto del individuo, se hace un recorrido conceptual de los eventos, de los eventos 2.0, de la narrativa transmedia y del transmedia branding para evaluar la explotacion de su potencial comunicativo en la comunicacion de marca. El objetivo principal se materializa en un analisis de la implementacion de las premisas de la narrativa transmedia en la comunicacion 2.0 de eventos. A partir de un muestreo intencional de trece destinos turisticos culturales espanoles, Sitios Patrimonio Mundial, se analiza la difusion de eventos y la interaccion generada en las redes sociales Facebook y Twitter mediante la metodologia del case studies. Los resultados revelan una gestion incipiente de la comunicacion transmedia de los eventos 2.0 en ambas redes sociales. Pese a ello, el analisis realizado y la bibliografia consultada corroboran el papel clave de los eventos en la creacion de marcas pais, territorio, turisticas, etc., y sustentan la principal aportacion de esta investigacion: la definicion de las premisas de implementacion de la narrativa transmedia en el ambito de los eventos. EnglishThe present research deals with the challenge posed by the use of events in the creation and development of tourist brands. From the perspective of the absolute protagonism of the individual, we make a conceptual overview of the events, events 2.0, storytelling transmedia and transmedia branding in order to evaluate the exploitation of its communicative potential in brand communication. The main goal is materialized in an analysis of the implementation of the premises of the storytelling transmedia in the 2.0 communication of events. From a deliberate sampling of thirteen Spanish cultural tourist destinations, World Heritage Sites, we analyze the dissemination of events and the interaction generated in the social networks Facebook and Twitter through the methodology of case studies. The results reveal an emerging management in the transmedia communication of events 2.0 in both social networks. In spite of this, the analysis carried out and the literature rewieved corroborate the key role of events in the creation of country, territory or tourist marks and support the principal contribution of this research: a series of recommendations for the implementation of transmedia storytelling in the events industry.
Primary cutaneous CD30‐positive large cell lymphoma

Primary cutaneous CD30‐positive large cell lymphoma is a recently described entity, recognized by immunohistochemical criteria. Spontaneous regression may occur and although relapses are frequent, most patients have a favourable prognosis. We describe a 83‐year‐old woman with a large ulcerated tumour on her right forearm, with 2 months evolution. Histological and immunohistochemical examination revealed a CD30‐positive large cell lymphoma. Systemic involvement was not detected. The tumour regressed spontaneously. Cutaneous relapses occurred one year later, all with spontaneous regression. After a 2‐year follow‐up period, the patient is well, without concurrent extracutaneous disease. This case is in keeping with other reports of spontaneous regression and favourable outcome of this type of cutaneous lymphoma.
Evidence That Light, Carbon Dioxide, and Oxygen Dependencies of Leaf Isoprene Emission Are Driven by Energy Status in Hybrid Aspen1

Leaf isoprene emission scales positively with light intensity, is inhibited by high carbon dioxide (CO2) concentrations, and may be enhanced or inhibited by low oxygen (O2) concentrations, but the mechanisms of environmental regulation of isoprene emission are still not fully understood. Emission controls by isoprene synthase, availability of carbon intermediates, or energetic cofactors have been suggested previously. In this study, we asked whether the short-term (tens of minutes) environmental control of isoprene synthesis results from alterations in the immediate isoprene precursor dimethylallyldiphosphate (DMADP) pool size, and to what extent DMADP concentrations are affected by the supply of carbon and energetic metabolites. A novel in vivo method based on postillumination isoprene release was employed to measure the pool size of DMADP simultaneously with the rates of isoprene emission and net assimilation at different light intensities and CO2 and O2 concentrations. Both net assimilation and isoprene emission rates increased hyperbolically with light intensity. The photosynthetic response to CO2 concentration was also hyperbolic, while the CO2 response curve of isoprene emission exhibited a maximum at close to CO2 compensation point. Low O2 positively affected both net assimilation and isoprene emission. In all cases, the variation in isoprene emission was matched with changes in DMADP pool size. The results of these experiments suggest that DMADP pool size controls the response of isoprene emission to light intensity and to CO2 and O2 concentrations and that the pool size is determined by the level of energetic metabolites generated in photosynthesis.
Intensive care unit physician staffing: financial modeling of the Leapfrog standard.

OBJECTIVE To evaluate from a hospital's perspective the costs and savings, over a 1-yr period, of implementing The Leapfrog Group's Intensive Care Unit Physician Staffing (IPS) standard compared with the existing standard of nonintensivist staffing in adult intensive care units. DESIGN Using published data, we developed a financial model of costs and savings for 6-, 12- and 18-bed intensive care units using conservative estimates for all variables. Sensitivity analyses, including a best-case and worst-case scenario, were performed to evaluate the impact of changing assumptions on the outcome of the model. SETTING Nonrural hospitals in the United States. PATIENTS : All adult intensive care unit patients. INTERVENTIONS The IPS standard requires that intensive care units have a dedicated intensivist present during daytime hours. Outside of these hours, an intensivist must be immediately available by pager, and a physician or "physician extender" must be in the hospital and able to immediately reach intensive care unit patients. MEASUREMENTS AND MAIN RESULTS Cost savings ranged from 510,000 to 3.3 million US dollars for 6- to 18-bed intensive care units. The best-case scenario demonstrated savings of 4.2-13 million US dollars. Under the worst-case scenario, there was a net cost of 890,000 to 1.3 million US dollars. CONCLUSIONS Financial modeling of implementation of the IPS standard using conservative assumptions demonstrated cost savings to hospitals. Only under worst-case scenario assumptions did intensivist staffing result in additional cost to hospitals. These economic findings must be interpreted in the context of significant reductions in patient morbidity and mortality rates also associated with intensivist staffing. Given the magnitude of its clinical and financial impact, hospital leaders should be asking "how to" rather than "whether to" implement The Leapfrog Group's ICU Physician Staffing standard.
Monensin inhibits the processing of herpes simplex virus glycoproteins, their transport to the cell surface, and the egress of virions from infected cells

HEp-2 cells or Vero cells infected with herpes simplex virus type 1 were exposed to the ionophore monensin, which is thought to block the transit of membrane vesicles from the Golgi apparatus to the cell surface. We found that yields of extracellular virus were reduced to less than 0.5% of control values by 0.2 microM monensin under conditions that permitted accumulation of cell-associated infectious virus at about 20% of control values. Viral protein synthesis was not inhibited by monensin, whereas late stages in the post-translational processing of the viral glycoproteins were blocked. The transport of viral glycoproteins to the cell surface was also blocked by monensin. Although the assembly of nucleocapsids appeared to be somewhat inhibited in monensin-treated cells, electron microscopy revealed that nucleocapsids were enveloped to yield virions, and electrophoretic analyses showed that the isolated virions contained immature forms of the envelope glycoproteins. Most of the virions which were assembled in monensin-treated cells accumulated in large intracytoplasmic vacuoles, whereas most of the virions produced by and associated with untreated cells were found attached to the cell surface. Our results implicate the Golgi apparatus in the egress of herpes simplex virus from infected cells and also suggest that complete processing of the viral envelope glycoproteins is not essential for nucleocapsid envelopment or for virion infectivity.
[Cerebral amyloid angiopathies].

PATHOLOGY FINDINGS Cerebral amyloid angiopathies are defined by the presence of amyloid deposits on the walls of cerebral vessels. These amyloid deposits are found in the media of arterioles of the leptomeninges and the cortex. They are sometimes associated with Alzheimer-type lesions. Overt amyloid vasculopathy characterized by lesions of the vascular wall with a media totally replaced by amyloid substance may be observed. CLINICAL EXPRESSION Lobular hemorrhage, often with recurrent episodes is the most frequent manifestation of cerebral amyloid angiopathy. Cerebral infarcts or leukoencephalopathy may also be observed. An association with cerebral angiitis has been reported in a few cases. SPORADIC OR FAMILIAL DISEASE: Several types can be distinguished depending on the sporadic or familiar nature of the disease pattern as well as the chemical make-up of the protein deposit. In sporadic cerebral amyloid angiopathy the deposit is composed of protein A beta, and in familial cases, of protein A beta, cystatin C, gelsosine or transthyretin. Mutation of the genes coding for amyloid substance constituents have been identified in diverse forms of familial cerebral amyloid angiopathy.
One-dimensional Si/Ge nanowires and their heterostructures for multifunctional applications—a review

Remarkable progress has been made in the field of one-dimensional semiconductor nanostructures for electronic and photonic devices. Group-IV semiconductors and their heterostructures have dominated the years of success in microelectronic industry. However their use in photonic devices is limited since they exhibit poor optical activity due to indirect band gap nature of Si and Ge. Reducing their dimensions below a characteristic length scale of various fundamental parameters like exciton Bohr radius, phonon mean free path, critical size of magnetic domains, exciton diffusion length etc result in the significant modification of bulk properties. In particular, light emission from Si/Ge nanowires due to quantum confinement, strain induced band structure modification and impurity doping may lead to the integration of photonic components with mature silicon CMOS technology in near future. Several promising applications based on Si and Ge nanowires have already been well established and studied, while others are now at the early demonstration stage. The control over various forms of energy and carrier transport through the unconstrained dimension makes Si and Ge nanowires a promising platform to manufacture advanced solid-state devices. This review presents the progress of the research with emphasis on their potential application of Si/Ge nanowires and their heterostructures for electronic, photonic, sensing and energy devices.
Effect of Selective Capsulorrhaphy on the Passive Range of Motion of the Glenohumeral Joint

Background: Capsulorrhaphy of the glenohumeral joint is a common surgical procedure for the treatment of instability caused by increased capsular laxity. The effect of capsulorrhaphy on the range of motion of the shoulder is poorly understood.Methods: We simulated localized capsular contractures by selective capsular plications in eight human cadaveric shoulders and studied the effect of such plications on the passive range of glenohumeral abduction, flexion, and external and internal rotation in different degrees of abduction. A 0.5 or 1-N-m torque was applied to the humerus, and the range of glenohumeral motion was measured with electronic goniometers in three planes and compared with those of the intact shoulder.Results: Anterosuperior capsular plication most markedly affected external rotation of the adducted arm, decreasing it by a mean of 30.1° (p < 0.0001). Anteroinferior plication significantly reduced abduction by a mean of 19.4° (p < 0.0001) and external rotation by a mean of 20.6° (p = 0.0046). Posterosuperior plication mostly limited internal rotation of the adducted arm (mean decrease, 16.1°, p = 0.0045). On the average, total anterior and total posterior plication each limited flexion by approximately 20° (p = 0.005) and abduction by ≥15° (p < 0.005), whereas total anterior plication limited external rotation by >30° (p £ 0.0002) and total posterior plication limited internal rotation by >20° (p < 0.0001). Total inferior capsular plication restricted abduction (by a mean of 27.7°, p = 0.0001), flexion, and rotation. Total superior plication restricted external rotation and flexion.Conclusions and Clinical Relevance: Localized plications of the glenohumeral joint capsule lead to predictable patterns of loss of glenohumeral mobility. If plication is planned, losses of movement can be anticipated. The findings of this study may assist surgeons in identifying the parts of the capsule that are contracted and that may need lengthening.
Market-based Short-term Electricity Price Forecast Based on EEMD and ARIMA

With the development and implementation of pilots in the Chinese spot market, the importance of electricity price forecasting to various entities in the electricity market is also constantly emerging. At the same time, with the continuous deepening of China's electricity market construction, the electricity market structure of each province gradually tends to be market-oriented, and the original electricity price forecasting method has been unable to adapt to the future changes in the structure of the electricity market. Based on the characteristics of real-time electricity prices in the spot market, this study proposes a short-term electricity price mixed forecasting model based on ARIMA and EEMD to predict the 24-hour electricity price. This hybrid model uses the ARIMA model to predict the time series decomposed by EEMD, which improves the stationarity of the electricity price series and improves the accuracy. Using MATLAB and SPSS tools, reference the electricity price data of the America PJM day-ahead market to verify the proposed prediction model. The results show that the hybrid model can significantly improve the forecast accuracy of electricity prices compared with the traditional ARIMA method. Keywords—Electricity price forecast; EEMD; ARIMA; Mixed model; Electricity market