Miracle-dz/UniFAR-FTUs huggingface dataset:
[Isolation of quizalofop-p-ethyl-degrading bacteria from soil by DGGE-colony in situ hybridization].

Naturally occurring bacteria isolates capable of metabolizing pesticides have received considerable attention because they offer the possibility of both environmentally friendly and in situ remediation. The effect of herbicide quizalofop-p-ethyl on bacterial community in soil was analyzed using the technique of PCR-DGGE for isolating strains biodegrading quizalofop-p-ethyl. Results indicated that the soil bacterial community structures significantly changed after adding quizalofop-p-ethyl. The bacterial diversity of soil showed an increasing-decreasing-increasing trend. The largest changes occurred in the 9th day and then became stabilized. According to the sequencing results of bands in DGGE profiles, it inferred that members of bacterial genera Pseudomonas, Massilia and Burkholderia had tolerance to quizalofop-p-ethyl, and the potential for degradation. These microbial groups could be used to isolate and screen as the indigenous microbial resources to reduce pesticide residues. Digoxigenin-labeled probes had been synthesized based on the sequencing results of bands in the DGGE profiles, and three bacterial strains capable of biodegrading quizalofop-p-ethyl were isolated from soil by colony in situ hybridization technique. The strain named L1 was able to utilize quizalofop-p-ethyl as the sole source of carbon. The strain was identified as Pseudomonas sp. , based on the phylogenetic analysis of 16S rRNA. The degrading ability of strain L1 in minimal medium with quizalofop-p-ethyl was investigated by HPLC. The quizalofop-p-ethyl content decreased by almost 50% after 7 days, and the biomass of strain L1 increased while the content of quizalofop-p-ethyl was decreased. This confirmed that the strain L1 had the capacity of degradation. This result provided a basis for future research on degradation mechanism and functional genes.
Stereoselective Glucuronidation of Bupropion Metabolites In Vitro and In Vivo

Bupropion is a widely used antidepressant and smoking cessation aid in addition to being one of two US Food and Drug Administration–recommended probe substrates for evaluation of cytochrome P450 2B6 activity. Racemic bupropion undergoes oxidative and reductive metabolism, producing a complex profile of pharmacologically active metabolites with relatively little known about the mechanisms underlying their elimination. A liquid chromatography-tandem mass spectrometry assay was developed to simultaneously separate and detect glucuronide metabolites of (R,R)- and (S,S)-hydroxybupropion, (R,R)- and (S,S)-hydrobupropion (threo) and (S,R)- and (R,S)-hydrobupropion (erythro), in human urine and liver subcellular fractions to begin exploring mechanisms underlying enantioselective metabolism and elimination of bupropion metabolites. Human liver microsomal data revealed marked glucuronidation stereoselectivity [Clint, 11.4 versus 4.3 µl/min per milligram for the formation of (R,R)- and (S,S)-hydroxybupropion glucuronide; and Clmax, 7.7 versus 1.1 µl/min per milligram for the formation of (R,R)- and (S,S)-hydrobupropion glucuronide], in concurrence with observed enantioselective urinary elimination of bupropion glucuronide conjugates. Approximately 10% of the administered bupropion dose was recovered in the urine as metabolites with glucuronide metabolites, accounting for approximately 40%, 15%, and 7% of the total excreted hydroxybupropion, erythro-hydrobupropion, and threo-hydrobupropion, respectively. Elimination pathways were further characterized using an expressed UDP-glucuronosyl transferase (UGT) panel with bupropion enantiomers (both individual and racemic) as substrates. UGT2B7 catalyzed the stereoselective formation of glucuronides of hydroxybupropion, (S,S)-hydrobupropion, (S,R)- and (R,S)-hydrobupropion; UGT1A9 catalyzed the formation of (R,R)-hydrobupropion glucuronide. These data systematically describe the metabolic pathways underlying bupropion metabolite disposition and significantly expand our knowledge of potential contributors to the interindividual and intraindividual variability in therapeutic and toxic effects of bupropion in humans.
Review Articles: Uremic Malnutrition: New Insights Into an Old Problem

ABSTRACT Uremic malnutrition is highly prevalent and is associated with poor clinical outcomes in end‐stage renal disease (ESRD) patients. Inadequate diet and a state of persistent catabolism play major roles in predisposing these patients to uremic malnutrition and appear to have an additive effect on overall outcome. Recent studies highlight the existence of a complex syndrome involving chronic inflammation, metabolic abnormalities, and hormonal derangements contributing to the increased morbidity and mortality observed in ESRD patients. Novel strategies such as appetite stimulants, anti‐inflammatory drugs, and anabolic hormones along with conventional nutritional supplementation may provide potential interventions to improve clinical outcome in ESRD patients.
Advanced Path Control Method for Filtering Secure Method in Sensor Networks

Summary In many wireless sensor network applications, sensor nodes that have a limited battery power are deployed in open and unattended environments. Owing to these features, an adversary can compromise the deployed sensor nodes and easily inject fabricated reports into the sensor network through the compromised nodes. This attack not only depletes a limited energy resource but also gives a false alarm for deceiving base station. Recently, filtering-based secure methods have been proposed to protect sensor networks from this type of attack. In these schemes, forwarding nodes verify all event reports to detect and drop a fabricated report. To verify received event reports, sensor nodes consume a significant amount of energy. In this paper, we propose an advance path control method by using a fuzzy system. The proposed method can conserves consumption energy and reduce latency. The sufficient resilience and energy efficiency of the proposed method are shown by the simulation results.
Comparison of concanavalin A reactive determinants on isolated haemocytes of parasite-infected and non-infected freshwater crayfish.

The specific binding of concanavalin A (Con A) to semigranular (SG) and granular (G) cells of the crayfishes Pacifastacus lelliusculus and Astacus astacus was shown by in vitro agglutination of fixed haemocytes and by direct labelling with fluorescein isothiocyanate-conjugated Con A (FITCCon A). When non-fixed cell monolayers from both species of crayf~sh were treated with FITC-Con A, capping of G cells was observed and nearly all of the SG cells showed patching. The percentage of capforming G cells from Psorospermium haeckelii-infected A. astacus w a s clearly higher than that of noninfected crayfish. Also in the signal crayfish P. leniusculus w h ~ c h carries the crayfish plague parasite Aphanomyces astaci within the cuticle, a high percentage of cap-forming G cells was present. In A. astacus which received a Zymosan injection, a higher percentage of cap formation of G cells was observed compared with that of control crayfish. These results show that the presence of parasites alters the binding pattern of Con A to the haemocyte surface dnd this altered binding pattern suggests that these cells have become activated for cellular defence reactions within the host haemolymph.
Soft and hard biometrics fusion for improved identity verification

Traditional biometric systems that are based on single biometric usually suffer from problems like unacceptable error rates, spoof attacks and insufficient population coverage. To improve system's reliability, two or more biometrics of the same identity could be combined in a manner that enhances the efficiency of the system. Multi-biometric systems integrate typical physiological (fingerprint, iris, face.etc.) and behavioral (e.g. gait, voice, signature.etc.) traits. However, other features like color of skin, color of irises and height can also be included to enhance the overall performance. In this paper, a framework for integrating a soft biometric like the color of a human iris within a multi modal biometric system that combines hard biometrics such as fingerprint and iris texture. Steerable pyramid filters and multi-channel log-Gabor filters are suggested here for extracting proper features of the fingerprints and iris textures respectively. Experimental results, considering two different fusion strategies, have shown a significant improvement in the performance compared to the results of the standalone combined fingerprint and iris matchers.
Development and Function of the Blood-Brain Barrier in the Context of Metabolic Control

Under physiological conditions, the brain consumes over 20% of the whole body energy supply. The blood-brain barrier (BBB) allows dynamic interactions between blood capillaries and the neuronal network in order to provide an adequate control of molecules that are transported in and out of the brain. Alterations in the BBB structure and function affecting brain accessibility to nutrients and exit of toxins are found in a number of diseases, which in turn may disturb brain function and nutrient signaling. In this review we explore the major advances obtained in the understanding of the BBB development and how its structure impacts on function. Furthermore, we focus on the particularities of the barrier permeability in the hypothalamus, its role in metabolic control and the potential impact of hypothalamic BBB abnormities in metabolic related diseases.
Manipulation Control with Dynamic Tactile Sensing

In this paper we describe ongoing work toward eventdriven dextrous manipulation. In this context, the events are primarily determined through tactile and force/torque sensing. We begin with a review of recent work in tactile event detection and its role in the control of manipulation. We then consider control issues, focusing on the problem of accomplishing smooth transitions as the constraints, dynamic equations and control objectives change from one phase of a manipulation task to the next. Smooth transitions are essential in dextrous manipulation because of the typically low inertias of the grasped object and the fingers Ñ the object accelerates quickly and the fingertip sensors produce large signals in response to disturbances at the contacts. Finally, we describe a language that we are developing to facilitate programming of dextrous manipulation tasks with multiple control modes and tactile sensors.
Finite-time frequency synchronization in microgrids

This paper proposes a finite time frequency controller that synchronizes the microgrid frequency to the nominal frequency and shares the active power among distributed generators (DG) based on their active power ratings. The finite-time control accelerates the synchronization speed and provides the synchronization for microgrid frequency and DG active powers in a finite period of time. In addition, the proposed control is distributed; i.e., each DG only requires its own information and the information of its neighbors on the communication network graph. The efficacy of the proposed finite-time frequency control subsequent to islanding process and load changes is verified for an islanded microgrid test system.
Molecular heterogeneity of Krabbe disease

Krabbe disease (globoid cell leukodystrophy) is an autosomal recessive neurodegenerative disorder that a†ects both the central and peripheral nervous system due to an enzymatic defect of galactocerebrosidase (GALC). Following its cloning, many mutations in the galactocerebrosidase gene have been reported, but the correlation between phenotype and genotype was not clear in many cases. In this study we further investigated the molecular defects in another 10 patients (6 Japanese and 4 non-Japanese), using cultured skin Ðbroblasts, and found 10 mutations, of which 8 were novel, including a nonsense mutation (W647X) and 7 missense mutations (G43R, S52F, T262I, Y319C, W410G, R515H, T652R) in the coding region. Some phenotype-speciÐc mutations were found but the other mutations were private. Mutations reported so far have been distributed over the whole GALC gene and it is difficult to speculate on functional domains of the GALC protein and phenotypically speciÐc regions.
Why Hysterectomy Rate are Lower in India

Compared to a higher frequency of hysterectomy (HT; 10-20%) in other countries(1) a lower rate (4-6%) has been reported from India. (2–5) However, the reasons for this lower rate have not been adequately researched. The present study was therefore planned to estimate the rate of HT in the adult women of study area, and to ascertain the factors associated with decision making regarding HT.
It’s What You Say and How You Say It: Exploring Textual and Audio Features for Podcast Data

Podcasts are relatively new media in the form 001 of spoken documents or conversations with a 002 wide range of topics, genres, and styles. With 003 a massive increase in the number of podcasts 004 and their listener base, it is beneficial to un-005 derstand podcasts better, to derive insights into 006 questions such as what makes certain podcasts 007 more popular than others or which tags help in 008 characterizing a podcast. In this work, we pro-009 vide a comprehensive analysis of hand-crafted 010 features from two modalities, i.e., text and 011 audio. We explore multiple feature combina-012 tions considering podcast popularity prediction 013 and multi-label tag assignment as proxy down-014 stream tasks. In our experiments, we use doc-015 ument embeddings, affective features, named 016 entities, tags, and topics as the textual features , 017 while multi-band modulation and traditional 018 speech processing features constitute the audio 019 features . We find the audio feature prosody and 020 textual affective features, sentiment and emo-021 tions are significant for both the downstream 022 tasks. We observe that the combination of tex-023 tual and audio features helps in improving per-024 formance in the popularity prediction task. 025
Computing Approximate Certain Answers over Incomplete Databases

Certain answers are a widely accepted semantics of query answering over incomplete databases. Since their computation is a coNP-hard problem, recent research has focused on developing evaluation algorithms with correctness guarantees, that is, techniques computing a sound but possibly incomplete set of certain answers. In this paper, we show how novel evaluation algorithms with correctness guarantees can be developed leveraging conditional tables and the conditional evaluation of queries, while retaining polynomial time data complexity.
EMPREENDEDORISMO SOCIAL: ESTUDO BIBLIOMÉTRICO SOBRE A PRODUÇÃO NACIONAL E INTERNACIONAL

Este estudo realizou um mapeamento da producao cientifica nacional e internacional do periodo compreendido entre 2010 e 2015 sobre empreendedorismo social. O presente trabalho emprega metodos bibliometricos para analisar bases de dados cientificas, e apresenta dados acerca da quantidade de artigos publicados e de periodicos encontrados; autores que mais publicaram; autores mais citados; temas recorrentes relacionados ao empreendedorismo social e metodos de pesquisa mais utilizados. Diante dos resultados, foi possivel verificar que a producao cientifica geral sobre o tema proposto apresentou um declinio em 2014, seguido por um crescimento em 2015. Alem disso, nota-se a predominância de publicacoes norte-americanas, enquanto as publicacoes nacionais encontram-se em fase de consolidacao teorica, buscando bases conceituais para que possam contribuir e se tornar relevantes para este campo do conhecimento.
The genetics of parallel evolution: a case study using thermal and non-thermal ecotypes of Mimulus guttatus from Yellowstone National Park

ii Acknowledgements.iii List of tables.v List of figures.v Introduction.1 Materials & Methods.8 Results.19 Discussion.31 Conclusion.43 References.47 Tables.54 Figure legends.57 Figures.61
Shotgun Sequencing

Shotgun sequencing is a laboratory technique for determining the DNA sequence of an organism's genome. T he method involves breaking the genome into a collection of small DNA fragments that are sequenced individually. A computer program looks for overlaps in the DNA sequences and uses them to place the individual fragments in their correct order to reconstitute the genome. Qeios · Definition, November 27, 2019
Vacuolar ATPase Regulates Zymogen Activation in Pancreatic Acini*

Supramaximal concentrations of cholecystokinin or its analogue caerulein have been shown to stimulate the proteolytic activation of zymogens within the pancreatic acinar cell and initiate acute pancreatitis. Previous studies suggest that a low pH compartment might be required for activation. To test this hypothesis, the effects of agents that modulate intracellular pH on caerulein-induced trypsin and chymotrypsin activation were studied. Pretreatment of pancreatic acini with the proto-ionophore monensin (10 μm) and the weak base chloroquine (40 μm) inhibited activation. Pre-incubation with the vacuolar ATPase (V-ATPase) inhibitors bafilomycin A1 and concanamycin A also decreased activation in a concentration-dependent manner with 50% inhibition at ∼50 and 25 nm, respectively. Caerulein stimulation caused a time- and concentration-dependent translocation of soluble V-ATPase V1 subunits to a membrane fraction, a marker of V-ATPase activation. Carbachol also stimulated translocation at supramaximal concentrations. Elevation of cytosolic Ca2+ by thapsigargin was sufficient to induce translocation. Thus, stimulation of V-ATPase activity appears to be required for agonist-induced zymogen activation in the pancreatic acinar cell.
A Zebrafish Model of Cryptococcal Infection Reveals Roles for Macrophages, Endothelial Cells, and Neutrophils in the Establishment and Control of Sustained Fungemia

ABSTRACT Cryptococcal meningoencephalitis is a fungal infection that predominantly affects immunocompromised patients and is uniformly fatal if left untreated. Timely diagnosis is difficult, and screening or prophylactic measures have generally not been successful. Thus, we need a better understanding of early, asymptomatic pathogenesis. Inhaled cryptococci must survive the host immune response, escape the lung, and persist within the bloodstream in order to reach and invade the brain. Here we took advantage of the zebrafish larval infection model to assess the process of cryptococcal infection and disease development sequentially in a single host. Using yeast or spores as infecting particles, we discovered that both cell types survived and replicated intracellularly and that both ultimately established a sustained, low-level fungemia. We propose that the establishment and maintenance of this sustained fungemia is an important stage of disease progression that has been difficult to study in other model systems. Our data suggest that sustained fungemia resulted from a pattern of repeated escape from, and reuptake by, macrophages, but endothelial cells were also seen to play a role as a niche for cryptococcal survival. Circulating yeast collected preferentially in the brain vasculature and eventually invaded the central nervous system (CNS). As suggested previously in a mouse model, we show here that neutrophils can play a valuable role in limiting the sustained fungemia, which can lead to meningoencephalitis. This early stage of pathogenesis—a balanced interaction between cryptococcal cells, macrophages, endothelial cells, and neutrophils—could represent a window for timely detection and intervention strategies for cryptococcal meningoencephalitis.
Iron kinetics with emphasis on iron overload.

BODY IRON IN MAN is estimated at 35 to 45 mg/kg or a total of about 3 g.1 The major portion of body iron, some 30 mg/kg, is held in the circulating red cell mass and erythroid marrow as hemoglobin. The only other fraction of quantitative significance is storage iron, amounting to another 15 mg/kg in the adult male and varying from 0 to 10 mg/kg in the female. The manner in which body iron is maintained and its movement within the body become important in understanding anemia and iron overload states. For the purposes of this discussion, three aspects of iron exchange will be considered: exchange between man and his environment, internal iron exchange and, more specifically, storage iron exchange.
Association between unmet needs and quality of life of cancer patients: A population-based study

Abstract Background. Two conceptually different morbidity outcomes unmet needs and health-related quality of life are used to identify cancer patients in need of clinical attention and to evaluate rehabilitation programmes. The knowledge on the interrelation between unmet needs and health-related quality of life is scarce. This paper studies the hypothesis that patient-perceived unmet needs of rehabilitation during the cancer trajectory are associated with decreased quality of life. Material and methods. Based on registers, a Danish population-based cohort of adult, incident, mixed-site cancer patients diagnosed between 1 October 2007 and 30 September 2008 was established. At 14 months following diagnosis participants completed a questionnaire including health-related quality of life (EORTC QLQ C-30), psychological distress (POMS-SF), and unmet needs with regard to physical, emotional, family-oriented, sexual, work-related, and financial problems. Unmet needs were assessed through six ad hoc questions. Results. Questionnaires were received from 3439 of 4947 patients, resulting in a response rate of 70%. The three most frequent cancer types were breast (28.4%), prostate (14.6%) and colorectal cancer (15.2%). Overall, 60.1% expressed an unmet need of rehabilitation in at least one area, physical and emotional problems being the most frequent (40.0% and 37.5%). For all scales of the EORTC QLQ C-30 and POMS, significant adjusted mean differences were observed between patients with unmet needs in at least one area and patients with no unmet needs (p-values < 0.001). These differences were well above levels usually considered clinically relevant. Further, impairment increased with increasing number of areas in which unmet needs were reported. Discussion. We confirmed the hypothesis that patient-perceived unmet needs of rehabilitation during the cancer trajectory are associated with decreased quality of life. This study supports the use of unmet needs questions to identify patients in need of clinical attention. Interventions reducing cancer patients’ perceived needs of rehabilitation may enhance quality of life.