Miracle-dz/UniFAR-FTUs huggingface dataset:
PTPL1/FAP-1 Negatively Regulates TRIP6 Function in Lysophosphatidic Acid-induced Cell Migration*

The LIM domain-containing TRIP6 (Thyroid Hormone Receptor-interacting Protein 6) is a focal adhesion molecule known to regulate lysophosphatidic acid (LPA)-induced cell migration through interaction with the LPA2 receptor. LPA stimulation targets TRIP6 to the focal adhesion complexes and promotes c-Src-dependent phosphorylation of TRIP6 at Tyr-55, which creates a docking site for the Crk Src homology 2 domain, thereby promoting LPA-induced morphological changes and cell migration. Here we further demonstrate that a switch from c-Src-mediated phosphorylation to PTPL1/Fas-associated phosphatase-1-dependent dephosphorylation serves as an inhibitory feedback control mechanism of TRIP6 function in LPA-induced cell migration. PTPL1 dephosphorylates phosphotyrosine 55 of TRIP6 in vitro and inhibits LPA-induced tyrosine phosphorylation of TRIP6 in cells. This negative regulation requires a direct protein-protein interaction between these two molecules and the phosphatase activity of PTPL1. In contrast to c-Src, PTPL1 prevents TRIP6 turnover at the sites of adhesions. As a result, LPA-induced association of TRIP6 with Crk and the function of TRIP6 to promote LPA-induced morphological changes and cell migration are inhibited by PTPL1. Together, these results reveal a novel mechanism by which PTPL1 phosphatase plays a counteracting role in regulating TRIP6 function in LPA-induced cell migration.
A real-valued MUSIC algorithm with forward/backward technique

This paper presents a novel real-valued multiple signal classification (RV-MUSIC) algorithm, which tremendously reduces the computation complexity than the complex one by a factor of four. The covariance matrix of the sensor output is firstly transformed into a real-valued covariance matrix via unitary forward/backward (FB) technique. Then the MUSIC algorithm in real domain is used to estimate the direction of arrival (DOA) by utilizing the symmetry properties of array structure. The proposed algorithm can solve problem with multiple incident signals in one-or two-dimensional scenarios. Simulation results show the effectiveness of the proposed methods.
Vers une distribution des MDP à grande échelle : étude de cas des voies navigables

Les reseaux de voies navigables devraient subir des changements importants en raison d'une volonte d'augmenter le trafic naval et des effets du changement climatique. Ces changements necessitent une gestion adaptative et resiliente de la ressource en eau et requierent donc une planification plus intelligente. Un modele representatif du reseau, utilisant des MDPs, est propose et teste afin d'optimiser la gestion de l'eau. Il fournit des resultats prometteurs, le modele propose permet de coordonner plusieurs entites sur plusieurs pas de temps de facon a eviter les inondations et secheresses dans le reseau. Cependant, la solution proposee ne permet pas de passer a l'echelle et n'est pas utilisable dans une application reelle. Les avantages et limitations de plusieurs approches de la litterature qui pourrait permettre de passer a l'echelle sont presentes et discutes sous le prisme de notre etude de cas.
Static magnetic fields affect cell size, shape, orientation, and membrane surface of human glioblastoma cells, as demonstrated by electron, optic, and atomic force microscopy

It is common knowledge that static magnetic fields (SMF) do not interact with living cells; thus, fewer studies of SMF compared with variable magnetic fields are carried out. However, evidence demonstrated that SMF affect cellular structures. To investigate the effect of exposure to increasing doses of SMF on cell morphology, human glioblastoma cells were exposed to SMF ranging between 80 and 3,000 G (8 and 300 mT).
Which patients talk about stressful life events and social problems to the general practitioner?

Background. Life events and social problems are common in primary-care attenders and contribute to the high rate of emotional distress observed in this setting but are often not disclosed during the consultation. Physicians' characteristics associated with patients' disclosure of psychosocial problems are well documented, but less empirical evidence is available on the contribution of patients' variables. Method. Logistic regression analyses were performed on a set of clinical and psychosocial data from 319 primary-care attenders with stressful life events and/or social problems in the presence or absence of emotional distress, in order to identify the variables that predict disclosure of such problems during the consultation. Results. Two-thirds of patients with stressful life events and social problems had mentioned them to their GP. In both sexes a positive attitude about confiding and emotional distress were the best predictors of confiding. In men they accounted for 76% of correct predictions and, in women, together with past confiding, long-lasting relationship with GP, and coexistence of life events and social problems, for 81%. A positive confiding attitude was related in males to age and severity of medical condition and in females to age and experience of past confiding. Conclusions. In view of the high prevalence of positive attitude towards confiding the efforts by GPs should focus on the reduction of disclosure thresholds. This would require increased abilities to elicit psychosocial information and show emphatic understanding of patients' life difficulties. By contrast there is a need to promote a positive attitude in confiding that concerns only a minority of primary-care patients. More data are needed for shaping more specific strategies for these patients.
The relationship of coagulation factors to clinical complications of acute pancreatitis.

Alterations in coagulation factors have been reported during acute pancreatitis. Therefore the relationship of coagulation measurements to complications of pancreatitis was evaluated prospectively in 35 patients in whom 130 serial coagulation profiles were performed, consisting of fibrinogen, platelet count (PC), fibrinogen-fibrin-related-antigen (FR-antigen), prothrombin time (PT), partial thromboplastin time, thrombin time, euglobulin clot lysis, and Factors II, V and VII-X levels. During attacks of acute pancreatitis, over-all mean initial fibrinogen and PC of 268 mg. per 100 ml. and 214,000 per cubic millimiter rose significantly (p less than 0.005) to peaks of 362 mg. per 100 ml. and 477,800 per cubic millimeter by day 6 to 10. Mean initial FR-antigen of 4.8 microgram per milliliter rose to peak 7.4 microgram per milliliter on day 5. In 21 patients with mild pancreatitis, mean highest fibrinogen, PC, FR-antigen, and PT were 329 mg. per 100 ml. , 361,500 per cubic millimeter, 5.3 microng per milliliter and 14.1 seconds. These values were significantly higher (p less than 0.05 to 0.01) in patients with severe pancreatitis, being 422 mg. per 100 ml. 528,000 per cubic millimeter, 13.7 microng per milliliter, and 15.5 seconds, respectively. Evaluation of the relationship of coagulation measurements to early clinical features showd that mean initial fibrinogen levels were significantly higher (p less than 0.05 to 0.01) in patients with initial amylase greater than 1,000 Somogyi units percent, serum glutamic oxaloacetic transaminase (SGOT) greater than 250 S.F. U. percent, and initial 72 hour PAO2 less than 75 mm. Hg. Early hypoxemia also correlated (p less than 0.05) with elevated initial FR-antigen levels. Impaired early renal function correlated (p less than 0.01) with elevated initial PC only. Early hypocalcemia did not correlate with coagulation measurements. These findings demonstrate that marked changes in coagulation parameters occur during acute pancreatitis and are related to over-all morbidity. Correlation of early coagulation measurements with amylase levels and with respiratory, renal, and hepatic dysfunction suggests that enzyme-related intravascular coagulation may be implicated in the pathogenesis of these complications of pancreatitis.
A novel method that improves sensitivity of protein detection in PAGE and Western blot

We have developed a simple and inexpensive method that improves sensitivity of protein and antigen detection in standard PAGE procedures. Our technique uses a sample microloader device with a funnel‐like structure, filled with a 4% stacking gel. When attach to the top of a polyacrylamide slab gel, the proteins in a sample are concentrated by electrophoresis into a small volume as they emerge from the device's narrow outlet. Our microloader has several advantages over previous devices, including simple assembly, high versatility, and absence of cross‐contamination between lanes. Addition of this device to a slab gel results in a fivefold increase in the sensitivity of antigen detection in a Western blot. As a result, less protein is required for loading and signal detection. Our protocol is a straightforward modification of a standard experimental technique, and is especially useful when only limited sample quantities are available.
Health behaviors of smokers, ex-smokers, and never smokers in an HMO.

BACKGROUND The preponderance of evidence suggests that smokers do not engage in health-promoting lifestyles compared to nonsmokers. Few studies, however, have considered possible differences in health behaviors among smokers at different stages of intention to change. In this paper we examined the relationship of smoking stage of change to health behaviors in an HMO population. METHODS Data were obtained from a baseline health status and preventive service evaluation survey mailed to 8000 HMO members over the age of 40. The sample was stratified based on the presence of one or more chronic diseases. Regression analyses were performed adjusting for covariates and weighting based on the stratification. RESULTS Smokers appear to vary by stage of change in their practice of health-promoting behaviors. After controlling for age, gender, professional advice, chronic disease, and education, smokers classified in early stages of change demonstrated significantly fewer positive health practices compared to never smokers. A consistent pattern emerged across health behaviors. CONCLUSIONS Smokers in the earliest stages of change appear to have the most room for improvement compared to ex-smokers and never smokers. Behavior change other than smoking could be an opportunity to engage early stage smokers.
Impact of simulation training on time to initiation of cardiopulmonary resuscitation for first-year pediatrics residents.

BACKGROUND Pediatrics residents have few opportunities to perform cardiopulmonary resuscitation (CPR). Enhancing the quality of CPR is a key factor to improving outcomes for cardiopulmonary arrest in children and requires effective training strategies. OBJECTIVE To evaluate the effectiveness of a simulation-based intervention to reduce first-year pediatrics residents' time for 3 critical actions in CPR: (1) call for help, (2) initiate bag-mask ventilation, and (3) initiate chest compressions. METHODS A prospective study involving 31 first-year pediatrics residents at a children's hospital assigned to an early or late (control) intervention group. Residents underwent baseline assessment followed by repeat evaluations at 3 and 6 months. Time to critical actions was scored by video review. A 90-minute educational intervention focused on skill practice was conducted following baseline evaluation for the early-intervention group and following 3-month evaluation for the late-intervention group. Primary outcome was change in time to initiating the 3 critical actions. Change in time was analyzed by comparison of Kaplan-Meier curves, using the log-rank test. A 10% sample was timed by a second rater. Agreement was assessed using intraclass correlation (ICC). RESULTS There was a statistically significant reduction in time for all 3 critical actions between baseline and 3-month evaluation in the early intervention group; this was not observed in the late (control) group. Rater agreement was excellent (ICC ≥ 0.99). CONCLUSIONS A simulation-based educational intervention significantly reduced time to initiation of CPR for first-year pediatrics residents. Simulation training facilitated acquisition of critical CPR skills that have the potential to impact patient outcome.
Surgical wound infections in the intensive care unit: the nurse's role.

Surgical wound infections are increasing in frequency. They have a negative effect on length of stay among intensive care unit (ICU) patients and patients on other wards. The reported incidence of surgical wound infections ranges from 15% to 38%; commonly associated microorganisms include Staphylococcus aureus, coagulase-negative Staphylococcus, Enterococcus, and Escherichia coli. Risk factors for the development of a surgical wound infection include type of procedure and reason for surgical intervention, presence of comorbidities, and operating room environment. Interventions for the prevention of surgical wound infections begin in the preoperative process, and they continue throughout the perioperative and postoperative period, and even after the patient is discharged home. Prevention and management of surgical wound infections requires evidence-based care in both the ICU and on the surgical wards. This article reviews knowledge of the causes of surgical wound infections, and implications for prevention and management.
Leptin is cleared from the circulation primarily by the kidney.

OBJECTIVE This study was performed to determine the pharmakocinetics of recombinant leptin in lean rats and to test the hypothesis that the kidneys play an important role in the clearance of leptin from the circulation. DESIGN 126I-leptin was administered by bolus intravenous injection. Blood samples were taken at various time points ranging from 1-180 min after administration and assayed for leptin. Pharmacokinetic parameters were determined in normal animals and after either bilateral nephrectomy or bilateral ureteral ligation. RESULTS Leptin was eliminated from the circulation following a three compartment model. The importance of the kidneys to the systemic clearance of leptin was studied by administrating leptin to binephrectomized rats. The systemic clearance of leptin in anephric rats was only 19% of that calculated for control animals. In order to assess the role of glomerular filtration, both ureters were ligated 5 h before leptin administration. Ureteral ligation reduced the systemic clearance of leptin by 30%. CONCLUSION These findings demonstrate that the short half life of leptin in the circulation is mainly determined by efficient renal clearance which is mediated in part by glomerular filtration.
A Single Chip Integrated Sol-Gel Thin Film LC Sensor for Measuring Moisture in ppm Level

A simple single chip integrated moisture sensor capable of wireless operation through inductive coupling has been proposed. The structure consists of planar inductor and parallel plate capacitor fabricated by sol-gel thin film of γ-Al2O3 (5 μm thick). The sensor chip is capable of measuring ppm level moisture in gaseous environment. The resonance frequency shift of the sensor in the presence of moisture excited by a high frequency ac signal is measured by impedance analyzer (4294A Agilent). The frequency sensitivity of the structure for a moisture range from 100 to 5000 ppm is nearly 119 Hz/ppm. The response and recovery times are 7 and 24 s, respectively. The experimental results show that the output is linear (4.2%), reproducible and stable. Device can be applied for wireless moisture measurement in ppm level.
Identification of secreted phosphoprotein 1 gene as a new rheumatoid arthritis susceptibility gene.

OBJECTIVE To evaluate the contribution of the SPP1 rs11439060 and rs9138 polymorphisms, previously reported as autoimmune risk variants, in the rheumatoid arthritis (RA) genetic background according to anti-citrullinated protein antibodies (ACPAs) status of RA individuals. METHODS We analysed a total of 11,715 RA cases and 26,493 controls from nine independent cohorts; all individuals were genotyped or had imputed genotypes for SPP1 rs11439060 and rs9138. The effect of the SPP1 rs11439060 and rs9138 risk-allele combination on osteopontin (OPN) expression in macrophages and OPN serum levels was investigated. RESULTS We provide evidence for a distinct contribution of SPP1 to RA susceptibility according to ACPA status: the combination of ≥3 SPP1 rs11439060 and rs9138 common alleles was associated mainly with ACPA negativity (p=1.29×10(-5), ORACPA-negative 1.257 (1.135 to 1.394)) and less with ACPA positivity (p=0.0148, ORACPA-positive 1.072 (1.014 to 1.134)). The ORs between these subgroups (ie, ACPA-positive and ACPA-negative) significantly differed (p=7.33×10(-3)). Expression quantitative trait locus analysis revealed an association of the SPP1 risk-allele combination with decreased SPP1 expression in peripheral macrophages from 599 individuals. To corroborate these findings, we found an association of the SPP1 risk-allele combination and low serum level of secreted OPN (p=0.0157), as well as serum level of secreted OPN correlated positively with ACPA production (p=0.005; r=0.483). CONCLUSIONS We demonstrate a significant contribution of the combination of SPP1 rs11439060 and rs9138 frequent alleles to risk of RA, the magnitude of the association being greater in patients negative for ACPAs.
Extensive Variation in Intracellular Symbiont Community Composition among Members of a Single Population of the Wood-Boring Bivalve Lyrodus pedicellatus (Bivalvia: Teredinidae)

ABSTRACT Shipworms (wood-boring bivalves of the family Teredinidae) harbor in their gills intracellular bacterial symbionts thought to produce enzymes that enable the host to consume cellulose as its primary carbon source. Recently, it was demonstrated that multiple genetically distinct symbiont populations coexist within one shipworm species, Lyrodus pedicellatus. Here we explore the extent to which symbiont communities vary among individuals of this species by quantitatively examining the diversity, abundance, and pattern of occurrence of symbiont ribotypes (unique 16S rRNA sequence types) among specimens drawn from a single laboratory-reared population. A total of 18 ribotypes were identified in two clone libraries generated from gill tissue of (i) a single specimen and (ii) four pooled specimens. Phylogenetic analysis assigned all of the ribotypes to a unique clade within the γ subgroup of proteobacteria which contained at least five well-supported internal clades (phylotypes). By competitive quantitative PCR and constant denaturant capillary electrophoresis, we estimated the number and abundance of symbiont phylotypes in gill samples of 13 individual shipworm specimens. Phylotype composition varied greatly; however, in all specimens the numerically dominant symbiont belonged to one of two nearly mutually exclusive phylotypes, each of which was detected with similar frequencies among specimens. A third phylotype, containing the culturable symbiont Teredinibacter turnerae, was identified in nearly all specimens, and two additional phylotypes were observed more sporadically. Such extensive variation in ribotype and phylotype composition among host specimens adds to a growing body of evidence that microbial endosymbiont populations may be both complex and dynamic and suggests that such genetic variation should be evaluated with regard to physiological and ecological differentiation.
A Reduced Progenitor Pool Population Accounts for the Rudimentary Appearance of the Septum, Medial Pallium and Dorsal Pallium in Birds

To date, most studies comparing birds and mammals have focused on the similarities in brain development, architecture and connectivity. However, major differences in size, anatomy and organization exist in the telencephalon of adult birds and mammals. For instance, the septum, medial pallium and dorsal pallium of birds appear rudimentary compared with those of mammals. To identify the developmental processes that give rise to this difference in size and anatomy of the septum, medial pallium and dorsal pallium, the thickness of the ventricular zone that encompasses these regions was measured in embryonic birds (i.e. chickens, sparrows) and mammals (i.e. rabbits, hedgehogs, shrews, platypus). Cumulative bromodeoxyuridine (BrdU) labeling in chickens at embryonic day 7 and 8 was also used to examine levels of cell proliferation in the ventricular zone of the septum, medial pallium and dorsal pallium. The study’s main finding is that the ventricular zone of the septum, medial pallium and dorsal pallium is thinner in birds than in mammals. In chickens, the septum, medial pallium and dorsal pallium ventricular zone harbor few proliferating (i.e. BrdU+) cells. Collectively, these findings suggest that a reduced progenitor pool population account for the ‘rudimentary’ appearance of the avian septum, medial pallium and dorsal pallium.
Security of Mobile Single Sign-On: A Rational Reconstruction of Facebook Login Solution

While there exist many secure authentication and authorization solutions for web applications, their adaptation in the mobile context is a new and open challenge. In this paper, we argue that the lack of a proper reference model for Single Sign-On (SSO) for mobile native applications drives many social network vendors (acting as Identity Providers) to develop their own mobile solution. However, as the implementation details are not well documented, it is difficult to establish the proper security level of these solutions. We thus provide a rational reconstruction of the Facebook SSO flow, including a comparison with the OAuth 2.0 standard and a security analysis obtained testing the Facebook SSO reconstruction against a set of identified SSO attacks. Based on this analysis, we have modified and generalized the Facebook solution proposing a native SSO solution capable of solving the identified vulnerabilities and accommodating any Identity Provider.
Details of the ultrastructure of Rickettsia prowazekii grown in the chick yolk sac

Details of the Ultrastructure of Rickettsia prowazekii Grown in the Chick Yolk Sac R. L. ANACKER, E. G. PICKENS,1 AND D. B. LACKMAN2 Rocky Mountain Laboratory, National Institute ofAllergy and Infectious Diseases, Hamilton, Montana 59840 Received for publication 8 May 1967 The fine structure of Rickettsia prowazekii as M. Shestopalova, Acta Virol. 10:260, 1966; R. G. revealed in thin sections of infected monkey Bird, N. Kordova, and J. R6hacek, Acta Virol. kidney cells, yolk sac of embryonated chicken 11:60, 1967). In our studies, we have observed eggs, louse gut, or tick hemocytes has recently three additional morphological features of the
Effects of Inorganic Seeds on Secondary Organic Aerosol (SOA) Formation

Atmospheric aerosol has significant influences on human health (Kaiser, 2005), visibility degradation (Cheng et al., 2011), and climate change (Satheesh and Moorthy, 2005). It was found that organic aerosols (OA) was the most abundant component of atmospheric aerosol (He et al., 2001) and more than 50% of the total OA are secondary organic aerosols (SOA) (Duan et al., 2005). SOA are produced from the oxidation of volatile organic compounds (VOCs) followed by gas-particle partitioning of the semivolatile organic products. Among the various VOCs, aromatic hydrocarbons are one type of SOA precursors which have drawn the most attention due to their abundance in the air and high SOA contribution to urban atmospheres (Lewandowski et al., 2008). Toluene and m-xylene are the two of the most abundant aromatic hydrocarbon species.