Miracle-dz/UniFAR-FTUs huggingface dataset:
Quantitative Analysis of Methyl-Parathion Pesticide in Presence of Enzyme Substrate Using Tapered Fiber Optic Biosensor

Application of a non-adiabatic tapered fiber optic biosensor for real-time, label-free detection of methyl-parathion pesticide (MPT) in presence of acetylthiocholine iodide (ATCh) is presented. The limit of detection of MPT in the presence of 1 mM ATCh as an enzyme substrate is found to be as low as <inline-formula> <tex-math notation="LaTeX">$23\times 10\,\,^{-{9}}$ </tex-math></inline-formula> M (S/N=3). The proposed sensor shows a dynamic range of 2.3 nm and a working range of 0.12 to <inline-formula> <tex-math notation="LaTeX">$31~\mu \text{M}$ </tex-math></inline-formula>. More importantly, it has the capability of quantifying the binding constants of MPT to the enzyme acetylcholinesterase (AChE) by performing just a single experiment. This can be done through the kinetic analysis of the analyte-enzyme reaction in the presence/absence of ATCh. The results show an association rate of <inline-formula> <tex-math notation="LaTeX">$3.835\times 10\,\,^{{4}}\,\,\text{M}^{-{1}}\text{s}^{-{1}}$ </tex-math></inline-formula> and a dissociation rate of 0.2745 s<sup>−1</sup> for MPT-AChE binding in presence of ATCh which lead to a value of <inline-formula> <tex-math notation="LaTeX">$1.4\times 10\,\,^{{5}}\,\,\text{M}^{-{1}}$ </tex-math></inline-formula> for equilibrium binding constant and <inline-formula> <tex-math notation="LaTeX">$7.16~\mu \text{M}$ </tex-math></inline-formula> for dissociation constant. Detection of MPT in the tap and river water at low levels has been achieved and the sensor specificity examined against different substances. Based on the obtained results, our biosensor can pave the way toward low-cost, real-time, label-free, and quantitative determination of organophosphate pesticides in real samples.
Potential Availability of Dense Mammogrphy for Prevention of Breast Cancer in Korean Women

Breast cancer is the second leading type of incident cancer in Korean women. The patterns of incidence rates by age group during recently 10 years make doubt about a cohort effect. While there is strong evidence that dense mammography is a risk factor for breast cancer in Western women, there has been only one case-control study for Korean women. Dense mammography is known risk factor for breast cancer, and the proportion of dense mammography in Korean women varied by age group. Authors tried to find a pattern between the proportion of dense mammography and the incidence rate of breast cancer after 10 years by age group. The annual change from the peak age group into the next age group in both curves showed the same slope. These suggest that dense mammography might be a risk factor for breast cancer in Korean women. Some large-scale longitudinal studies are needed to evaluate the association, but the results can be used for controlling the prevention of breast cancer in Korean women.
Solutions of fractional kinetic equation and the generalized Galué type Struve function

Abstract This paper aims to establish extensive form of the fractional kinetic equation involving generalized Galué type Struve function using the technique of Sumudu transform. Further, numerical values of the results are interpreted and their graphical interpretation of the solutions by employing MATLAB is given. The results obtained here are quite general and capable of yielding a very large number of known and new results.
ARTSENS® Pen—portable easy-to-use device for carotid stiffness measurement: technology validation and clinical-utility assessment

Objective: The conventional medical imaging modalities used for arterial stiffness measurement are non-scalable and unviable for field-level vascular screening. The need for an affordable, easy-to-operate automated non-invasive technologies remains unmet. To address this need, we present a portable image-free ultrasound device—ARTSENS® Pen, that uses a single-element ultrasound transducer for carotid stiffness evaluation. Approach: The performance of the device was clinically validated on a cohort of 523 subjects. A clinical-grade B-mode ultrasound imaging system (ALOKA eTracking) was used as the reference. Carotid stiffness measurements were taken using the ARTSENS® Pen in sitting posture emulating field scenarios. Main results: A statistically significant correlation (r > 0.80, p < 0.0001) with a non-significant bias was observed between the measurements obtained from the two devices. The ARTSENS® Pen device could perform highly repeatable measurements (with variation smaller than 10%) on a relatively larger percentage of the population when compared to the ALOKA system. The study results also revealed the sensitivity of ARTSENS® Pen to detect changes in arterial stiffness with age. Significance: The easy-to-use technology and the automated algorithms of the ARTSENS® Pen make it suitable for cardiovascular risk assessment in resource-constrained settings.
How can governance support collaborative innovation in the public sector? A systematic review of the literature

Collaborative innovation has been increasingly adopted by the public sector to address complex issues and improve its efficiency. Despite the developments in the literature on this subject, recent studies still indicate the need to investigate how public governance could be organized to support collaborative processes of innovation. Through a systematic review of 36 empirical studies, this article aims to contribute to bridging this gap by identifying the characteristics that should form governance in order to support the collaborative innovation process in the public sector. The results show that the establishment of relationships of trust, supported by technology tools and promoted by leaders committed to well-established goals, is a characteristic of governance that has a positive influence on collaborative innovation processes. However, there is still a need to develop more studies that indicate the characteristics of governance that may contribute to the implementation of innovation and not just to its creation processes. Points for practitioners Collaboration has been shown to be a more positive strategy for innovation in the public sector in comparison with strategies that seek isolation or competition. However, it is necessary to establish governance mechanisms that contribute to the involvement of stakeholders and to the achievement of the intended results so that this strategy becomes successful. By reviewing the results of prior empirical experiences, this article identifies that in order for collaborative innovation processes in the public sector to be successful, governance must predict: the participation of top management and managers with decision-making power; the setting of clear goals and needs; the generation of useful innovation that benefits stakeholders; and the establishment of tools that facilitate communication, interaction, and the sharing of information and knowledge. This information provides managers and public policymakers with key indicators, learned from prior mistakes and accomplishments, for the implementation of this innovation strategy in their organizations.
Formal Verification of Control System Software

The verification of control system software is critical to a host of technologies and industries, from aeronautics and medical technology to the cars we drive. The failure of controller software can cost people their lives. This book provides control engineers and computer scientists with an introduction to the formal techniques for analyzing and verifying this important class of software. Too often, control engineers are unaware of the issues surrounding the verification of software, while computer scientists tend to be unfamiliar with the specificities of controller software. The book provides a unified approach that is geared to graduate students in both fields, covering formal verification methods as well as the design and verification of controllers. It presents a wealth of new verification techniques for performing exhaustive analysis of controller software. These include new means to compute nonlinear invariants, the use of convex optimization tools, and methods for dealing with numerical imprecisions such as floating point computations occurring in the analyzed software. As the autonomy of critical systems continues to increase—as evidenced by autonomous cars, drones, and satellites and landers—the numerical functions in these systems are growing ever more advanced. The techniques presented here are essential to support the formal analysis of the controller software being used in these new and emerging technologies.
Single molecule imaging reveals cFos is capable of binding to and diffusing on DNA tightropes independently of cJun

AP-1 proteins are members of the basic leucine zipper (bZIP) protein family of dimeric transcription factors, responsible for controlling many integral cellular processes. These proteins form dimers with each other, and their aberrant expression can lead to a number of cancer types. The oncogenic transcription factor AP-1 binds its target TRE site (5′TCA[G/C]TGA), however the physical mechanism of how this is achieved is not understood. Such an understanding is essential to know how these proteins function, and could offer the potential to uncover new drug targets. The archetypal AP-1 complex is formed by cFos and cJun, which heterodimerise via their bZIP domains. Here, we set out to investigate how these proteins interact with DNA using a real-time single molecule fluorescence imaging approach. Using DNA tightropes as a substrate, we determine that the AP-1 bZIP dimers cJun:cFos and cJun:cJun rapidly scan DNA using a 1D diffusional search with an average diffusion constant of 0.14 μm2s-1 and 0.26 μm2s-1 respectively. Remarkably, we also found that cFos was able to bind to and diffuse on DNA (0.29 μm2s-1) both as a monomer and homodimer. Periods of diffusion were punctuated by pauses, suggesting a mechanism for how AP-1 may rapidly find its target sites on DNA. Taken together the results we have obtained indicate a considerably more complex and graded interaction between cFos, cJun and DNA than has been reported previously.
SILD synthesis of the efficient and stable electrocatalyst based on CoO-NiO solid solution toward hydrogen production

Currently, nanocrystalline NiO is well known as one of the best non-noble metal electrode material with low overpotential (OP) but mediocre stability. On the contrary, CoO has remarkable stability but the high values of OP. In this work, a method is proposed to achieve the stability of nickel oxide-based electrode materials while maintaining a low OP via the synthesis of a nanocrystalline CoO–NiO solid solution. Nanocrystals of CoO–NiO solid solution were synthesized by successive ionic layer deposition (SILD). XRD, SEM, and EDX analysis show that the CoO– NiO sample consists of 3 – 5 nm isometric crystallites of the solid solution mentioned above and Ni/Co ratio is equal to 45.4 % / 54.6 % at. Electrochemical investigation of the nanocrystalline CoO–NiO solution as electrode material shows OP values of −240 mV at a current density (CD) of 10 mA/cm, Tafel slope values of 78 mV/dec for hydrogen production from water-ethanol solution (10 % vol.) and high cyclic stability – only 3 mV degradation at 10 mA/cm after 100 cycles of cyclic voltammetry. Thus, it was shown that the synthesis of a solid solution within the proposed approach makes it possible to maintain the high electrocatalytic properties inherent in NiO, but with high stability in a wide range of overpotential and in the high cyclic load inherent in CoO.
Immune activation and clinical responses following long-term infusion of anti-GD2 antibody ch14.18/CHO in combination with interleukin-2 in high-risk neuroblastoma patients.

10028 Background: A less toxic treatment using long-term infusion (LTI) of anti-GD2antibody ch14.18/CHO and subcutaneous interleukin-2 (IL-2) may improve outcome in high risk neuroblastoma (NB). Methods: 53 NB patients received 5/6 cycles of 6x106 IU/m2 s.c. IL-2 (d1-5; 8-12), LTI of 100 mg/m2 ch14.18/CHO (d8-17) and 160 mg/m2 oral 13-cis-RA (d19-32) in a single center program. Usage of i.v. -morphine and scores established for pediatric pain assessment were documented. Effector cells (NK- and T-cell subsets), ch14.18/CHO levels and GD2 specific killing of neuroblastoma cells by ADCC, CDC, and whole blood were analyzed. KIR/KIRL mismatch and Fcγ-receptor polymorphisms were determined with a validated PCR-based method for KIR, HLA, FCGR2A (H131R), -3A (V158F) and -3B (NA1/NA2). Clinical response was assessed following INRG criteria by mIBG, MRI/CT, BM and catecholamines. Results: LTI of ch14.18/CHO translated into a decreasing degree of i.v. morphine usage allowing for treatment in the outpatient setting. T.
Factores de riesgo para sobrepeso y obesidad en lactantes Risk factors for overweight and obesity in infants

ABSTRACTIntroduction: At one year of age, factors that favor the develo-pment of obesity and other metabolic disorders in later stages of life are present. Objective: To identify perinatal, societal, and dietary risk factors for excess weight and obesity in 12 months infants. Subjects and methods: Case-control studies with infants under the care of Family Medicine Units. These cases studied were overweight or obese children, and control cases were children at a healthy weight. Each group included 152 infants, who were evaluated based on their responses to a questionnaire, their re-cords, and perinatal, societal, and dietary variables. Results: The variables with statistical signiﬁ cance were high pre-gestational weight, high gestational weight gain (OR 1.79-3.88), absence of breastfeeding and exclusive formula feeding (OR 2.02 and 1.75). Conclusion: In the presence of pre-pregnancy obesity and exclusive formula feeding, there is a 64.4% greater risk of becoming overweight or obese at 12 months old.Key words: infants, excess weight, weight problems, obesity, perinatal, diet.
Intelligence in Tourist Destinations Management: Improved Attention-based Gated Recurrent Unit Model for Accurate Tourist Flow Forecasting

Accurate tourist flow forecasting is an important issue in tourist destinations management. Given the influence of various factors on varying degrees, tourist flow with strong nonlinear characteristics is difficult to forecast accurately. In this study, a deep learning method, namely, Gated Recurrent Unit (GRU) is used for the first time for tourist flow forecasting. GRU captures long-term dependencies efficiently. However, GRU’s ability to pay attention to the characteristics of sub-windows within different related factors is insufficient. Therefore, this study proposes an improved attention mechanism with a horizontal weighting method based on related factors importance. This improved attention mechanism is introduced to the encoding–decoding framework and combined with GRU. A competitive random search is also used to generate the optimal parameter combination at the attention layer. In addition, we validate the application of web search index and climate comfort in prediction. This study utilizes the tourist flow of the famous Huangshan Scenic Area in China as the research subject. Experimental results show that compared with other basic models, the proposed Improved Attention-based Gated Recurrent Unit (IA-GRU) model that includes web search index and climate comfort has better prediction abilities that can provide a more reliable basis for tourist destinations management.
Structural adaptation of oxygen tolerance in 4-hydroxybutyrl-CoA dehydratase, a key enzyme of archaeal carbon fixation

Autotrophic microorganisms that convert inorganic carbon into organic matter were key players in the evolution of life on Earth. As the early atmosphere became oxygenated, microorganisms needed to develop mechanisms for oxygen protection, especially those relying on enzymes containing oxygen-sensitive metal clusters (e.g., Fe-S). Here we investigated how 4-hydroxybutyryl-CoA dehydratase (4HBD) - the key enzyme of the 3-hydroxypropionate/4-hydroxybutyrate (HP/HB) cycle for CO2-fixation - adapted as conditions shifted from anoxic to oxic. 4HBD is found in both anaerobic bacteria and aerobic ammonia-oxidizing archaea (AOA). The oxygen-sensitive bacterial 4HBD and oxygen-tolerant archaeal 4HBD share 59 % amino acid identity. To examine the structural basis of oxygen tolerance in archaeal 4HBD, we determined the atomic resolution structure of the enzyme. Two tunnels providing access to the canonical [4Fe-4S] cluster in oxygen-sensitive bacterial 4HBD were closed with four conserved mutations found in all aerobic AOA and other archaea. Further biochemical experiments and molecular dynamics simulations support our findings that restricting access to the active site is the key to oxygen tolerance, explaining how active site evolution drove a major evolutionary transition. Significance statement Autotrophy (primary production) was the first life strategy on Earth. Before photosynthesis (using solar energy to fix carbon dioxide), life relied on chemical reactions for energy. These chemosynthetic reactions are present in all domains of life, including archaea possessing the most energy-efficient carbon fixation pathway - the 3-hydroxypropionate/4-hydroxybutyrate cycle. This efficiency results from enzyme modifications, including enhanced enzyme stability and catalysis of multiple reactions. We reveal the first structure of aerobic 4-hydroxybutyryl-CoA dehydratase (4HBD) from ammonia-oxidizing archaea. These archaea are among the most abundant organisms on the planet, and their 4HBD active site evolved oxygen tolerance to support aerobic metabolism. This modification can provide further insight into enzyme evolution on early earth, as photosynthesis developed and began oxygenating the atmosphere.
To profit or not to profit? That is the wrong question

In social economy research, the issue of ‘profit’ (whether to make and/or distribute it) is a dominant framing concept. We argue that this dominance (even negatively referenced) maintains the hegemony of financial capital and the ‘for-profit’ paradigm. By refusing to accept a definition that labels organisations in terms of what they are not, scholars of co-operative development, social enterprise and voluntary action can better understand the value-creating activities of third sector organisations (TSOs) on their own terms. We argue that scholars and policy makers are complicit in maintaining the dominance of financial capital and the for-profit paradigm when they adopt for-profit/non-profit language in debates about their field. The counter-narrative we offer is based on engagement with work from the International Integrated Reporting Council (IRC) and FairShares Association (FSA). By comparing six capitals defined by the IIRC with the FSA’s statement on ‘six forms of wealth’, we offer a new way to account for the wealth creation of co-operatives, (other) social enterprises and voluntary associations. This ‘for-purpose’ framework for TSOs “reclaims the conversation” by identifying the wealth creation of social enterprises in comparison with the wealth destruction of private companies locked into chrematistic accounting and reporting practices which focus on the pursuit of profit for its own sake. This allows the fundamental differences between for-purpose enterprises (primarily social) and not-for-purpose businesses (primarily financial) to be clearly articulated.
Disease activity and functional disability in rheumatoid arthritis patients

Celem pracy by3a ocena korelacji pomiêdzy aktywnoœci1 choroby a niepe3nosprawnoœci1 oraz innymi sk3adowymi jakoœci ¿ycia u pacjentów hospitalizowanych z powodu zaostrzenia reumatoidalnego zapalenia stawów. Wœród 169 pacjentów hospitalizowanych w Klinice Reumatologii, którzy zgodzili siê wype3niæ przygotowany kwestionariusz, 87% stanowi3y kobiety. U ka¿dego chorego jednorazowo oceniono liczbê bolesnych i obrzêkniêtych stawów (wg indeksu 28-stawowego), wartoœæ OB, stê¿enie CRP, d3ugoœæ trwania sztywnoœci porannej, ból i stan zdrowia w 100-mm skali VAS, nastêpnie wyliczono wskaŸnik DAS 28 (3 zmienne) jako sumaryczny wyk3adnik aktywnoœci choroby. W ocenie stopnia niepe3nosprawnoœci wykorzystano kwestionariusz HAQ oraz skalê Likerta dla wydolnoœci funkcjonalnej i zakresu wykonywanych zajêæ domowych. Dane analizowano za pomoc1 systemu statystycznego SAS. Mediany dla wieku i czasu trwania choroby wynosi3y odpowiednio 56 i 6 lat. Badana grupa charakteryzowa3a siê wysok1 aktywnoœci1 choroby (mediana DAS 28=5,43) oraz wysokim poziomem niepe3nosprawnoœci (mediana HAQ=1,88). Stwierdzono korelacjê pomiêdzy niepe3nosprawnoœci1 (HAQ) a aktywnoœci1 reumatoidalnego zapalenia stawów wyra¿on1 wspó3czynnikiem DAS 28 (r=0,44; p<0,0001), ogólnym stanem zdrowia (r=0,42; p<0,0001), aktywnoœci1 choroby w ci1gu ostatnich 6 mies. (r=0,41; p<0,0001) i w dniu oceny (r=0,37; p<0,0001), czasem trwania sztywnoœci porannej (r=0,40; p<0,0001), nasileniem bólu w skali VAS (r=0,33; p<0,0001), a tak¿e stê¿eniem CRP w surowicy (r=0,32; p<0,0001). WskaŸnik aktywnoœci choroby DAS 28 by3 równie¿ znamiennie statystycznie skorelowany z wszystkimi powy¿szymi czynnikami, w szczególnoœci z bólem (r=0,44; p<0,0001). Stwierdzono ponadto, S u m m a r y
Entrepreneurship Preference among University Students: an Evidence of Entrepreneurship Education Program

This study aims to answer the effectiveness of EEP toward entrepreneurial preference in Indonesia. Entrepreneurship Education Program (EEP) has become one of the main instruments of growth of many governments around the world, including the Indonesian government. Nevertheless, polemics and debates have arisen in Indonesia regarding its implementation effectiveness. Through this study, we also emphasize the utilization of family (parents’) work background to understand the effectiveness of EEP further. Surveys among university students were conducted in this research, particularly to those who were exposed to EEP during their studies. By using the Theory of Planned Behavior (TPB), the findings conveyed that EEP is indeed effective and in line with students’ entrepreneurial preferences. Of the three factors, only subjective norms have a contradicting relationship. Similar results occur using family work background, i.e., families with entrepreneurial-work backgrounds and families who do not have an entrepreneurial-work background. This research contributes to the behavioral theory, particularly in mitigating EEP polemics among researchers and practitioners, as well as assess the effectiveness of EEP.
A Review of Researches on Accounting in China Brought by Artificial Intelligence*

—In recent years, with the innovation and development of science and technology, the application of artificial intelligence technology in accounting field has become more and more extensive and in-depth. The integration of artificial intelligence and accounting is both an opportunity and a challenge. This paper combs the research literatures in the field of accounting brought by artificial intelligence, gets to know the basic situation of the research literatures, analyzes the application status, influence and coping strategies of artificial intelligence in accounting field, points out the limitations of existing research, and provides reference recommendations for the future research trend.
Review—Biomass Derived Carbon Materials for Electrochemical Sensors

Sensors have become integral part of our lives. Electrochemical sensors are the oldest and the most commercially used sensors. Biomass carbonization by pyrolysis and hydrothermal methods are discussed as a cost-effective strategy for fabrication of electrodes for electrochemical sensing applications. Porosity and surface area along with graphitic nature of bio derived carbon materials greatly affects the performance of electrochemical sensors. Various techniques are used to improve the surface properties so as to enhance the electrocatalytic behavior of working electrodes. Synthetic and bioderived carbon materials are compared for their electrochemical sensing applications.