Miracle-dz/UniFAR-FTUs huggingface dataset:
Long Non-Coding RNA Expression Profiling in Aging Rats with Erectile Dysfunction

Background/Aims: Erectile dysfunction (ED) in aged people remains a topic of interest to andrological physicians. Long non-coding RNAs (lncRNAs), which form the largest group of non-coding RNAs, have been shown to regulate various biological processes. The function of lncRNAs in age-related erectile dysfunction (A-ED) pathogenesis remains poorly understood. Methods: This study aims to assess the differential expression profiles of mRNAs and lncRNAs between A-ED and normal control (NC) samples. Using a second-generation lncRNA microarray, we detected a total of 8,744 lncRNAs and 13,585 coding transcripts. Results: We identified 608 up-regulated and 406 down-regulated lncRNAs in A-ED compared with NC samples, by setting a filter of fold-change >2.0. Gene Ontology and pathway analysis revealed that a muscle contraction disorder induced by abnormal ion channels might play a critical role in the pathogenesis of A-ED. Conclusion: Our results show significantly altered expression profiles of lncRNAs and mRNAs between A-ED and NC. This study may provide information for further research on A-ED and may be helpful for finding a new therapeutic target for A-ED.
Positive and negative coupling of the endothelin ETA receptor to Ca2+‐permeable channels in rabbit cerebral cortex arterioles

1 Arteriolar segments were isolated from pial membrane and studied within 10 h. Current‐clamp and voltage‐clamp measurements were made by patch‐clamp recording from smooth muscle cells within arterioles. [Ca2+]i was measured from the smooth muscle cell layer by digital imaging of emission from fura‐PE3 which was loaded into arterioles by pre‐incubation with the acetoxymethyl ester derivative. The external diameter of arterioles was measured using a video‐dimension analyser. 2 Endothelin‐1 (ET1) was a potent constrictor of isolated arterioles and induced a sustained depolarization up to ‐27 mV and reduced membrane resistance (EC50 140‐170 pm). At a constant holding potential of ‐60 mV ET‐1 induced a transient followed by a sustained inward current. ET1 inhibited L‐type voltage‐dependent Ca2+ current. 3 ET1 induced a transient followed by sustained elevation of [Ca2+]i. The sustained effect was dependent on extracellular Ca2+. It occurred at a constant holding potential of ‐60 mV and was not inhibited by the Ca2+ antagonists nicardipine (1 μM) or D600 (10 μM). Thapsigargin (1 μM) completely depleted Ca2+ from caffeine‐ and ET1‐sensitive sarcoplasmic reticulum but did not inhibit the ET1‐induced sustained elevation of [Ca2+]i. ET1 effects on [Ca2+]i were prevented by the ETA receptor antagonist BQ123 (cyclo‐D‐Asp‐Pro‐D‐Val‐Leu‐D‐Trp). 4 The data suggest that ETA receptors are negatively coupled to L‐type Ca2+ channels and positively coupled to receptor‐operated Ca2+‐permeable channels. Inhibition of L‐type Ca2+ channel activity may suppress autoregulation, and Ca2+ influx through receptor‐operated channels may have a major functional role in the potent long‐lasting constrictor effect of endothelin‐1 in the cerebral microcirculation.
A Multi-channel infrastructure based on DCF access mechanism for Wireless LAN Mesh Networks compliant with IEEE 802.11

The mesh topology based on the standard IEEE 802.11 for wireless LANs appears to be a very promising architecture for achieving a ubiquitous wireless Internet access in the future. However, the current IEEE 802.11 protocol is aimed at single access point (AP) environments and many problems related to the wireless meshed interconnection of APs and mobile terminals (MTs) remain to be solved. Some proposed solutions to build such mesh architectures are based on single-channel ad-hoc oriented schemes in which IEEE 802.11 protocol has been modified. The main problem with this type of schemes, however, lies in the very low performance of the single-channel architecture itself. The task group S of IEEE 802.11 is currently working out standards for IEEE 802.11-compliant mesh architectures in a number of usage scenarios including residential, office and campus/community/public access network but a lot of work remains to be done since the group was established just on last year. In this paper we propose a new multi-channel mesh architecture for hot zones which works using a distributed coordination function (DCF)-based technique for interconnecting APs. A major advantage of our proposed scheme is that, putting routing issues aside, it introduces no change into the MAC protocol of IEEE 802.11. Our simulations results obtained in OPNET show a good performance of our proposed scheme in terms of throughput and delay. We also show interesting results related with the size of packets and the mesh architecture itself that could lead to further research in the future
Exploring the Person-Centeredness of an Innovative E-Supportive System Aimed at Person-Centered Care: Prototype Evaluation of the Care Expert

Integrated in a multiphase development project, the Care Expert is a prototype of a novel e-supportive system aiming to mediate person-centered care in the context of outpatient oncology. At this early stage of development, the current study was conducted aiming at exploring the person-centeredness concept underlying the Care Expert version 1.0 and its usability for patients receiving outpatient chemotherapy for breast cancer. Within a user-centered design, we followed a mixed-methods approach entailing subjective assessment and diagnostic evaluation of the prototype. Four women undergoing outpatient chemotherapy participated in individual sessions and rated highly the system’s usability. Their accounts led to identifying three supportive functions: continuous communication, reinforcement of self-driven agency, and cooperative agency with a sense of being looked after. We discuss the results in relation to theoretical fields that might guide further the development of the supportive system and usability recommendations. Care Expert has the potential to mediate person-centered care in outpatient oncology. Nevertheless, additional cycles of iterative development with the software team and of participatory design focusing on oncology nurses’ perspectives are required before departing to the feasibility phase in intervention research.
Lymphocyte adhesion to psoriatic dermal endothelium: mechanism and modulation.

Psoriasis is characterized by the hyperproliferation of keratinocytes in the epidermis and the accumulation of activated CD4+ T lymphocytes in the upper dermis. We have recently tested the hypothesis that the abnormal endothelial proliferation in the dermal papillae of psoriatic lesions may be mechanistically linked to the expression of endothelial ligands capable of promoting lymphocytes binding and extravasation. The results indicated that specialized endothelial cells lining the post-capillary venules of psoriatic lesions are capable of promoting the selective adherence of human CD4+ T cells and its memory subset. In contrast, B cells, CD8+ T cells, and CD45RA+ T cells are deficient in their capacities to bind. The adhesion process is energy and calcium dependent and involves tissue-specific lymphocyte receptors, with LFA-1 molecules playing an accessory role. We concluded that transformation of the dermal endothelium into a lymphocyte-receptive phenotype by defined growth factors or cytokines may represent a positive feedback mechanism promoting lymphocyte migration into the diseased sites.
Summarizing Online Discussions by filtering posts

In this paper, we attempt to summarize online discussions by filtering posts. Selecting the highly related posts from the discussion boards leads to a summarized version of the discussion. Online Discussion Summarizer (ODS) is based on unsupervised information retrieval techniques. Four features are used in the summarization function; which are the term frequency inverse post frequency, title term frequency, description term frequency and author reputation. This paper shows that combining the four features in the same function results in higher accuracy than using each alone. ODS was able to summarize online discussions with an accuracy of 72%, precession of 83% and recall of 62%.
Multiplexing efficiency of high order MIMO in mobile terminal in different propagation scenarios

The multiplexing efficiency of 4×4 higher order MIMO with different MIMO topologies under different propagation scenarios in a mobile terminal is presented in this study. The mean effective gain, correlation coefficient and multiplexing efficiency of different MIMO topologies are compared and the impact from the angular spread of the incoming wave is also studied. The results are instructive for the high order MIMO antenna design in mobile terminal.
Cessation in the use of tobacco – pharmacologic and non‐pharmacologic routines in patients

Introduction: Approximately one‐third of the adult population in industrial countries and 70% in several Asian countries are daily smokers. Tobacco is now regarded as the world's leading cause of death. Approximately two‐thirds of lifelong smokers eventually die because of smoking. Smoking cessation is the most effective action to reduce mortality in patients with chronic obstructive pulmonary disease (COPD) and coronary heart disease.
Balancing ecological complexity in predictive models: a reassessment of risk models in the mountain pine beetle system

1. The nature of ecological risk assessment is to predict the probability of an event, such as extinction or invasion, in a location where the event has rarely occurred. This typically requires developing risk models from data on events in different locations. One perplexing challenge in developing these models is to find the optimal balance of model complexity that reflects the tactical details of a system, but is sufficiently strategic to be applicable under a wide range of situations. 2. Here we address the balance of complexity in risk models for the mountain pine beetle system. Mountain pine beetles (Dendroctonus ponderosae Hopkins) are destructive pests of pine forests in western North America. Much effort has gone into collecting empirical evidence and developing mechanistic models of infestation dynamics, which has resulted in a wealth of process-based information. Current risk models, however, are based solely on indices of stand susceptibility that do not incorporate much of this ecological understanding. In practice, current risk models have proven ineffective at predicting the risk or extent of an infestation. 3. We assemble an ecological framework of the beetle-host interaction that allows us to compare across phenomenological and mechanistic models. We demonstrate that current risk models predict only ranked risk among forest stands, as opposed to absolute risk, and thereby provide an explanation for their limited ability to predict risk in practice. By comparing existing models with the ecological framework, we identify the primary factors determining risk, and propose which dynamical processes should be modelled explicitly, and which might be strategically abstracted. 4. Synthesis and applications. Balancing model complexity in predictive risk models is challenging for systems with complex ecology and imperfect information. Here we draw together a wide range of empirical and modelling work in the mountain pine beetle system to develop a strategic framework of the ecological interactions. Through this framework, we demonstrate why current risk models have been ineffective in predicting risk, and suggest a starting point for future risk models that explicitly describe the dynamical processes necessary to predict absolute risk.
The influence of renal function on the renal clearance of morphine and its glucuronide metabolites in intensive-care patients.

1. The relationships between renal creatinine clearance and the renal clearances of morphine, morphine-3-glucuronide (M3G) and morphine-6-glucuronide (M6G) were studied in fifteen intensive-care patients who were receiving morphine sulphate by constant intravenous infusion and who had diverse renal function. 2. An arterial blood sample was collected before and after a 4-5 h urine collection. Plasma and urine concentrations of morphine, M3G and M6G were measured by h.p.l.c. Plasma binding of all three compounds in drug-free plasma from healthy volunteers was determined by ultrafiltration. Measured renal creatinine clearance (CLCr,meas) was calculated from plasma and urinary creatinine concentrations (from h.p.l.c.). Also, creatinine clearance was predicted (CLCr,pred) from routine laboratory determination of plasma creatinine (Jaffe method). 3. There were significant linear relationships (P less than 0.001) between CLCr,meas and the renal clearances of morphine, M3G and M6G. The unbound renal clearance of morphine exceeded CLCr,meas (P less than 0.002) while the unbound renal clearances of M3G and M6G did not differ from CLCr,meas (P greater than 0.5). 4. In ten of the patients who received a constant infusion of morphine for at least 6 h, the dose-normalised plasma concentrations of M3G and M6G increased with decreasing CLCr,pred. Significant (P less than 0.001) relationships were observed between the reciprocal of CLCr,pred and the dose-normalised plasma concentrations of M3G and M6G. 5. The results indicate the importance of renal function in determining the renal clearances and plasma concentrations of M3G and M6G during intravenous infusion with morphine in intensive-care patients.
Does Early Supplementation Affect Long-Term Breastfeeding?

The purpose of this secondary data analysis from two different samples was to examine the effect of early supplementation with manufactured milks on breastfeeding status at 20 weeks postpartum in mothers of healthy term infants. In two convenience samples of 120 and 223, respectively, breastfeeding mothers were followed up for 20 weeks postpartum or until weaning occurred. The breastfeeding rate at 20 weeks postpartum was significantly greater for mothers who reported feeding exclusively mother's milk the second week after delivery compared with mothers who breastfed and simultaneously supplemented with manufactured infant milks. Of the mothers in samples one and two who exclusively fed human milk during week 2 postpartum, 63.0% and 59.7%, respectively, were still breastfeeding at week 20, compared with 28.1 % and 24.2 %, respectively, who supplemented with artificial milks. There was no significant difference between these two groups of mothers and their intended duration of breastfeeding. Early introduction of supplemental bottles of artificial milks is associated with a decrease in the amount of human milk the infant receives as well as with early weaning.
P-CIP1, A Novel Protein That Interacts with the Cytosolic Domain of Peptidylglycine α-Amidating Monooxygenase, Is Associated with Endosomes*

The cytosolic domain of the peptide processing enzyme peptidylglycine α-amidating monooxygenase (PAM) contains signals that direct its trafficking in the secretory and endosomal pathways. Using the yeast two-hybrid system, Alam et al.(Alam, M. R., Caldwell, B. D., Johnson, R. C., Darlington, D. N., Mains, R. E., and Eipper, B. A. (1996) J. Biol. Chem. 271, 28636) identified three proteins that interact with a fragment of the PAM cytosolic domain containing these targeting signals. We cloned the rat and human cDNAs encoding PAM COOH-terminalinteractor protein-1 (P-CIP1). Both cDNAs contain an open reading frame that encodes a novel protein of 435 amino acids. The P-CIP1 protein is highly conserved from rat to human (85% identity) but does not display significant homology to proteins in the GenBank data base. In vitro, P-CIP1 interacts with the cytosolic domain of wild type PAM-1, but does not interact with mutant PAM-1 proteins that fail to target correctly when expressed in endocrine cells. P-CIP1 contains multiple consensus serine/threonine phosphorylation sites and a region predicted to form a coiled-coil at the COOH terminus. When expressed in endocrine cells or fibroblasts, P-CIP1 is distributed in a punctate pattern in the perinuclear area but does not significantly overlap the distribution of transfected wild type PAM-1. The distribution of P-CIP1 displays significant overlap with the distribution of the secretory carriermembrane proteins, internalized Texas Red-conjugated transferrin, and Rab11. The data suggest that P-CIP1 associates with vesicles in the recycling endosomal pathway, and may play a role in regulating the trafficking of integral membrane PAM.
Plasma damage immunity of thin gate oxide grown on very lightly N/sup +/ implanted silicon

Plasma damage immunity of gate oxide grown on very low dose (2/spl times/10/sup 13//cm/sup 2/) N/sup +/ implanted silicon is found to be improved compared to a regular gate oxide of similar thickness. Both hole trapping and electron trapping are suppressed by the incorporation of nitrogen into the gate oxide. Hole trapping behavior was determined from the relationship between initial electron trapping slope (IETS) and threshold voltage shifts due to current stress. This method is believed to be far more reliable than the typical method of initial gate voltage lowering during current stress.
Position Paper: Gastric Lavage

Gastric lavage should not be employed routinely, if ever, in the management of poisoned patients. In experimental studies, the amount of marker removed by gastric lavage was highly variable and diminished with time. The results of clinical outcome studies in overdose patients are weighed heavily on the side of showing a lack of beneficial effect. Serious risks of the procedure include hypoxia, dysrhythmias, laryngospasm, perforation of the GI tract or pharynx, fluid and electrolyte abnormalities, and aspiration pneumonitis. Contraindications include loss of protective airway reflexes (unless the patient is first intubated tracheally), ingestion of a strong acid or alkali, ingestion of a hydrocarbon with a high aspiration potential, or risk of GI hemorrhage due to an underlying medical or surgical condition. A review of the 1997 Gastric Lavage Position Statement revealed no new evidence that would require a revision of the conclusions of the Statement.
Recommended Internet Service Provider Security Services and Procedures

The purpose of this document is to express what the engineering community as represented by the IETF expects of Internet Service Providers (ISPs) with respect to security. It is not the intent of this document to define a set of requirements that would be appropriate for all ISPs, but rather to raise awareness among ISPs of the community’s expectations, and to provide the community with a framework for discussion of security expectations with current and prospective service providers.
Influence of child and adolescent psychiatric disorders on young adult personality disorder.

OBJECTIVE This study examines associations between childhood psychopathology and young adult personality disorder in a random sample of 551 youths, who were 9 to 16 years old at first assessment. METHOD Subjects were evaluated for DSM-III-R psychiatric disorders. Information was obtained prospectively from youths and their mothers at three points over 10 years. The predictive effects of prior axis I disorders and adolescent axis II personality disorder clusters A, B, and C on young adult personality disorder were examined in logistic regression analyses. RESULTS The odds of young adult personality disorder increased given an adolescent personality disorder in the same cluster. Prior disruptive disorders, anxiety disorders, and major depression all significantly increased the odds of young adult personality disorder independent of an adolescent personality disorder. In addition, comorbidity of axis I and axis II disorders heightened the odds of young adult personality disorder relative to the odds of a disorder on a single axis. CONCLUSIONS Assessment of personality pathology before late adolescence may be warranted. Childhood or adolescent axis I disorders may set in motion a chain of maladaptive behaviors and environmental responses that foster more persistent psychopathology over time. Identification and treatment of childhood disorder may help to reduce that risk.
Mid millimeter waves for broadband satellite communication 72– 100 GHz

We use high elevation satellite systems and 95% availability to extend the attractiveness of millimeter wave communications. Millimeter wave satellite communication in the 72-100 GHz region is indicated to be attractive for most of the Temperate Zone, with a 95% non rainy condition. The worldwide attenuation function is given (Appendix).
Study of WPT system for charging portable devices on a desk

Wireless power transfer (WPT) is interesting again because it is an effective technology to realize the ubiquitous society. WPT via magnetic resonant coupling is a new technology which power can be transferred via coupled magnetic resonant in the non-radiative near-field. A simple wireless desktop charging system via coupled magnetic resonant was proposed in this paper. This system contains two same structure minimize antennas which was embedded into the monitor and the portable electronic devices. The transfer efficiency related with different transfer conductions was simulated and analysed. Experimental results show that the power transfer efficiency is more than 70% when the distance between two antennas are 30mm. The receiving antenna can be also used as a communication antenna because the reflection coefficient of antenna is low when antenna resonant at high communication frequency bandwidth of UHF.
NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants

NDP kinases (NDPKs) are multifunctional proteins that regulate a variety of eukaryotic cellular activities, including cell proliferation, development, and differentiation. However, much less is known about the functional significance of NDPKs in plants. We show here that NDPK is associated with H2O2-mediated mitogen-activated protein kinase signaling in plants. H2O2 stress strongly induces the expression of the NDPK2 gene in Arabidopsis thaliana (AtNDPK2). Proteins from transgenic plants overexpressing AtNDPK2 showed high levels of autophosphorylation and NDPK activity, and they have lower levels of reactive oxygen species (ROS) than wild-type plants. Mutants lacking AtNDPK2 had higher levels of ROS than wild type. H2O2 treatment induced the phosphorylation of two endogenous proteins whose molecular weights suggested they are AtMPK3 and AtMPK6, two H2O2-activated A. thaliana mitogen-activated protein kinases. In the absence of H2O2 treatment, phosphorylation of these proteins was slightly elevated in plants overexpressing AtNDPK2 but markedly decreased in the AtNDPK2 deletion mutant. Yeast two-hybrid and in vitro protein pull-down assays revealed that AtNDPK2 specifically interacts with AtMPK3 and AtMPK6. Furthermore, AtNDPK2 also enhances the myelin basic protein phosphorylation activity of AtMPK3 in vitro. Finally, constitutive overexpression of AtNDPK2 in Arabidopsis plants conferred an enhanced tolerance to multiple environmental stresses that elicit ROS accumulation in situ. Thus, AtNDPK2 appears to play a previously uncharacterized regulatory role in H2O2-mediated MAPK signaling in plants.