Miracle-dz/UniFAR-FTUs huggingface dataset:
Oocyte Cryopreservation: Retrospective Analysis Of 5 Years Experience

OBJECTIVE: To analyze demographic and clinical data of patients who resorted to oocyte freezing between January 2014 and December 2018. STUDY DESIGN: Patients who applied to the Reproductive Endocrinology and Infertility Unit of Ankara University School of Medicine between January 2014 and December 2018 with the request of oocyte freezing were included in this study. The files and computer records of the patients were analyzed retrospectively and sociodemographic, clinical and laboratory data were evaluated. RESULTS: A total of 46 cycles were recorded in 40 patients over a 5-year period. The main indications were low ovarian reserve and/or advanced age (68.3%) and malignancy diagnosis (31.7%). There was a significant difference between elective fertility preservation and oncofertility preservation (Onco-FP) groups in terms of the age (38.4±4.7 vs 28.4±6.1; p=0.001). There was a significant difference between two groups in favor of oncofertility group in terms of anti-Mullerian hormone level, basal follicle-stimulating hormone level, antral follicle count, trigger day estradiol (E2) level, number of obtained oocytes, MII oocytes, and frozen oocytesCONCLUSION: According to our study, the most prominent oocyte cryopreservation indication was advanced age and/or low ovarian reserve. The number of oocytes collected from patients in the Onco-FP group and thus the number of frozen oocytes was significantly higher than in the elective fertility preservation group, due to younger ages and better ovarian reserve in the Onco-FP group. Abdominal administration of the technique is particularly important for virgin patients in our country. Oocyte freezing is a fertility protection method available in a wide range of indications for reproductive-aged women.
Enhancing cognitive and functional assessment for Alzheimer’s disease

iv Lay Summary . vii Abbreviation list . viii List of Tables . xix List of Figures . xxi Chapter I Introduction . 1 Part I . 1 Cognitive and functional decline in neurodegenerative disorders: where are we standing? 1 1.1 AD-Specific Markers: Associative memory tests . 3 Theory behind the associative memory tests . 4 1.2 AD-Specific Markers: The Temporary Memory Binding Task (TMB) . 5 Theories behind development of the TMB test . 6 Neuroanatomical distinction of relational and conjunctive associative memory . 8 Chapter I – Part II . 10 Everyday functional abilities: Measures, Barriers and Suggestions for Improvements. 10 1.3 Defining ADL scales. 10 1.4 New formats of ADL scales. 11 1.5 Self-reported vs. informant reports vs. performance measures . 12 1.6 Methodological issues . 13 1.7 How to overcome ADL challenges . 14 Chapter I – Part III . 16 1.8 Healthy ageing. 16 1.8. 1 Cognitive abilities in healthy ageing . 16
Immunolocalization of the ICE/Ced-3–Family Protease, CPP32 (Caspase-3), in Non-Hodgkin's Lymphomas, Chronic Lymphocytic Leukemias, and Reactive Lymph Nodes

Immunohistochemical analysis of the apoptosis-effector protease CPP32 (Caspase-3) in normal lymph nodes, tonsils, and nodes affected with reactive hyperplasia (n = 22) showed strong immunoreactivity in the apoptosis-prone germinal center B-lymphocytes of secondary follicles, but little or no reactivity in the surrounding long-lived mantle zone lymphocytes. Immunoblot analysis of fluorescence-activated cell sorted germinal center and mantle zone B cells supported the immunohistochemical results. In 22 of 27 (81%) follicular small cleaved cell non-Hodgkin's B-cell lymphomas, the CPP32-immunopositive germinal center lymphocytes were replaced by CPP32-negative tumor cells. In contrast, the large cell component of follicular mixed cells (FMs) and follicular large cell lymphomas (FLCLs) was strongly CPP32 immunopositive in 12 of 17 (71%) and in 8 of 14 (57%) cases, respectively, whereas the residual small-cleaved cells were poorly stained for CPP32 in all FLCLs and in 12 of 17 (71%) FMs, suggesting that an upregulation of CPP32 immunoreactivity occurred during progression. Similarly, cytosolic immunostaining for CPP32 was present in 10 of 12 (83%) diffuse large cell lymphomas (DLCLs) and 2 of 3 diffuse mixed B-cell lymphomas (DMs). Immunopositivity for CPP32 was also found in the majority of other types of non-Hodgkin's lymphomas studied. Plasmacytomas were CPP32 immunonegative in 4 of 12 (33%) cases, in contrast to normal plasma cells, which uniformly contained intense CPP32 immunoreactivity, implying downregulation of CPP32 in a subset of these malignancies. All 12 peripheral blood B-cell chronic lymphocyte leukemia specimens examined were CPP32 immunopositive, whereas 3 of 3 small lymphocytic lymphomas were CPP32 negative, suggesting that CPP32 expression may vary depending on the tissue compartment in which these neoplastic B cells reside. The results show dynamic regulation of CPP32 expression in normal and malignant lymphocytes.
S2k-Leitlinie „Akute infektiöse Gastroenteritis im Säuglings-, Kindes- und Jugendalter“ – AWMF Registernummer 068-003

Leitlinie der Gesellschaft für pädiatrische Gastroenterologie und Ernährung (GPGE) gemeinsam mit der Deutschen Gesellschaft für Kinderund Jugendmedizin (DGKJ), dem Berufsverband der Kinderund Jugendärzte (BVKJ), der Deutschen Gesellschaft für pädiatrische Infektiologie (DGPI), der österreichischen Gesellschaft für Kinderund Jugendheilkunde (ÖGKJ), der Deutschen Gesellschaft für Gastroenterologie, Verdauungsund Stoffwechselstörungen (DGVS), dem Arbeitskreis „Krankenhaus & Praxishygiene“ der AWMF und der Deutschen Gesellschaft für Pflegewissenschaft e. V. (DGPW)
Evolving therapeutic goals in Crohn's disease management

Summary The main objectives in Crohn's disease are to avoid disease complications and preserve the patient's quality of life. Early disease control and close monitoring with specific targets to reach might be the only way to change the disease course. In two decades, we have moved from clinical response to full remission (clinical and endoscopic remission) requiring a tight monitoring of both symptoms and objective signs of inflammation. This review summarizes the concepts of tight control and treat-to-target and their potential for disease modification.
Clinical and experimental approaches to dermal and epidermal repair : normal and chronic wounds : proceedings of the Third International Symposium on Tissue Repair, held in Miami, Florida, January 10-14, 1990

Clinical spectrum of chronic wounds anatomy, pathophysiology, problems, and solutions vascular factors governing heating in chronic wounds, T.Hunt if wounds could talk, G.Mulder clinical studies on growth factors in pressure sores - preliminary report, M.Robson et al the extracellular wound environment and its role in wound healing - characteristics of fetal repair, T.Krummel et al wound fluid - the growth environment, T.Hunt contemporary approaches to wound healing, wound dressings - current and future, W.Eaglstein et al assessment of wound healing speed in man, J.Viljanto.
Advanced glycation end products facilitate the proliferation and reduce early apoptosis of cardiac microvascular endothelial cells via PKCβ signaling pathway: Insight from diabetic cardiomyopathy

Objective: To investigate the effects of advanced glycation end products (AGEs) on the proliferation and apoptosis of cardiac microvascular endothelial cells (CMECs) in rats and their underlying signaling pathway. Methods: CMECs were isolated from Sprague–Dawley rats. We first examined the effects of AGEs on the proliferation and apoptosis of CMECs and then tested whether protein kinase C (PKC) β blockers could counteract the effects of AGEs. The PKC agonists phorbol 12-myristate 13-acetate (PMA) and PKCβ blockers were also used to verify whether PKC could act independently on CMECs. The receptor for AGEs (RAGE)–small interfering RNA (siRNA) transfection was used to verify the effect of AGEs on PKC. Following the above steps, we explained whether AGEs regulated the CMEC proliferation and early apoptosis through the PKCβ signaling pathway. Proliferation of CMECs was detected using the Cell Counting Kit-8 (CCK-8) assay, and early apoptosis was determined using the Annexin V- Fluorescein Isothiocyanate (FITC)/propidium iodide (PI) double staining. Expression of proliferation and apoptosis-related proteins and PKC phosphorylation were determined by western blotting analysis. Cell cycle distributions were assayed using a BD FACSCalibur cell-sorting system. Results: AGEs facilitated the proliferation of CMECs, upregulated phosphorylated extracellular signal regulated kinase (p-ERK), and accelerated the entry of cells from G1 phase to the S+G2/M phase, which was consistent with the upregulated cyclin D1 by AGEs. AGEs inhibited early apoptosis of CMECs by increasing the expression of survivin and decreasing the expression of cleaved-caspase3. All these effects can be reversed by PKCβ1/2inhibitors. In addition, AGE upregulated the RAGE expression and phosphorylation of PKCβ1/2 in CMECs, while the inhibition of RAGE reversed the phosphorylation, as well as the effects of AGEs on proliferation and apoptosis in CMECs. Conclusion: The study indicated that AGEs facilitated the proliferation and reduced early apoptosis of CMECs via the PKCβ signaling pathway.
To Study the Role of Rutin as Antioxidant and its Neuroprotective Effect in Type II Diabetic Retinopathy

: Type II Diabetic Retinopathy is most common cause of visual loss in the world and it is referred as a blinding complication of diabetes that damages retina of the eye. In hyperglycemic condition it is been reported there is reduction in synthesis of various neuroprotective factor in diabetic patients retina as comparison of non diabetic. Neuroprotective Factors includes pigment epithelial-derived factor, brain derived neurotrophic factor, nerve growth factor, somatostatin, and interstitial retinol-binding protein. Prior studies have discovered that T2DR is associated with neurodegeneration and apoptosis that take place in the retina and the hyperglycemia-induced overproduction of reactive oxygen species. Thus, oxidative stress has been regarded as key factor for causing ocular disease and induces local inflammation, mitochondrial dysfunction, and microvascular dysfunction, disruption of blood- retinal barrier and cell apoptosis of retinal capillary cells.
High-Throughput Functional Ex-Vivo Drug Testing and Multi-Omics Profiling in Patients with Acute Myeloid Leukemia

Acute myeloid leukemia (AML) is the most common leukemia in adults and the prognosis is dismal. The heterogeneity of driver mutations in AML from one patient to another is significant. Although new treatment targets and modalities have recently been introduced, a lack of therapies to cover the entire spectrum of molecularly distinct AML classes remains a challenge. Furthermore, often only a fraction of the patients carrying mutations actually respond to the drugs predicted to target such genomic subtypes in AML. Our question was if outcome can be improved by optimizing existing and emerging therapies? To address this, we applied direct functional drug testing ex vivo in patients with newly diagnosed AML, along with deep molecular profiling to identify new therapeutic and diagnostic opportunities for specific genetic subclasses. To date, 122 patient samples have been included in the study. We have collected AML patient mononuclear cells isolated from fresh bone marrow aspirates from the Karolinska University Hospital Huddinge and Uppsala University Hospital, or applied biobanked samples from the Swedish acute leukemia biobank. The cells from the patient are tested for 72h with up to 525 conventional and investigative oncology drugs in a 5-point concentration range to determine optimal treatment options. The test is based on bulk cell viability and the cells are cultured in bone marrow stroma-conditioned media. The data is compared to healthy bone marrow controls to filter out generally toxic drugs and combinations. Excess patient material is applied for multi-omics analysis, including genome sequencing, deep proteomic profiling, concentrations of soluble factors, bulk/single-cell RNA sequencing and mass cytometry. Our results indicate significant heterogeneity in functional drug responses across individual patients, even among those with the same founder mutations. We could identify potential targeted treatments for most patients based on the exvivo testing. For instance, patients that show resistance to the Bcl-2 inhibitor Venetoclax can be associated with a distinct drug sensitivity response pattern that includes drugs with different mechanisms of actions. Based on the clustering of drug response data in all clinical samples, we could identify groups of patients with similar global drug responses, and drugs with similar patterns of efficacy across all patients. Lehmann: Pfizer: Membership on an entity's Board of Directors or advisory committees; Abbive: Membership on an entity's Board of Directors or advisory committees; TEVA: Consultancy, Membership on an entity's Board of Directors or advisory committees. Kallioniemi:Medisapiens: Other: Co-founder and stockholder; Sartar Therapeutics: Other: Co-founder and stockholder; Astra-Zeneca: Other: Joint Grant; Pelago: Other: Joint Grant; Takara: Other: Joint Grant; Abbot: Other: Licensing income.
Psychomotor retardation in recurrent depression and the related factors

Purpose: The article aims at presenting the current state of knowledge concerning the relationship between psychomotor retardation, balance control and modulation of bioelectric brain activity and the patient’s mental state, temperament traits and selected cognitive functions in patients suffering from recurrent depressive disorder. Views: The article contains an overview of research published between 1998 and 2018 based on PubMed, Google Scholar databases on both the main symptoms of depression with particular focus on psychomotor functions, and the relationship between motor activity and temperament traits (in particular neuroticism, trait anxiety, depressive temperament and the dominance of the BIS – Behavioral Inhibition System over the BAS – Behavioral Activation System), as well as selected cognitive functions (e.g. processing speed, attention span, operating memory and visual-spatial memory). The latter, as reported by studies both in this group of patients (recurrent depression) and in other groups (e.g. schizophrenia) may also be lowered during the remission period. The article lists simple and easily available tools that have been used for years in neurological and psychological clinical work, along with quantitative methods of data collection (QEEG, actigraphy, posturography). Conclusions: In patients with recurrent depressive disorders, a change in motor functions is observed, manifested by a motor retardation and changes in the balance and gait dynamics, as well as changes in the quantitative assessment of bioelectric brain activity. The relationship between these biological parameters and clinical symptoms of depression and their usefulness as biomarkers of depression is currently being evaluated in an experimental study conducted by the authors.
The relationship between dietary intakes during pregnancy and incidence of postpartum depression: a case-control study

Purpose Post-partum depression (PPD) is a mood disorder that affects 20-40 per cent of women in their post-delivery period worldwide. The purpose of this paper is to compare dietary intakes of energy, macronutrients, cholesterol, saturated fatty acids (SFAs), mono-unsaturated fatty acids (MUFAs), poly-unsaturated fatty acids (PUFAs), some micronutrients and antioxidants in PPD patients with healthy controls. Design/methodology/approach This case-control study was conducted on 163 women in postpartum period (81 PPD and 82 non-PPD) using Edinburgh questionnaire for the diagnosis of PPD. Dietary nutrients intake was assessed using 147-item semi-quantitative food frequency questionnaire (FFQ). Portion sizes of food items were converted to grams per day. Logistic regression models were used to estimate the association between tertiles of dietary intakes with the odds ratio (OR) of PPD. Findings According to the fully adjusted model, highest tertile compared to lowest tertile dietary intake of SFAs [OR = 0.01; 95 per cent confidence interval (CI) = 0.00, 0.01, p = 0.001], MUFAs (OR = 0.01; 95 per cent CI = 0.00, 0.02, p < 0.001), total fats (OR = 0.01; 95 per cent CI =0.00, 0.01, p < 0.001) and cholesterol (OR = 0.06 ; 95 per cent CI = 0.01, 0.08, p < 0.001), thiamine (OR = 0.01; 95 per cent CI = 0.00, 0.01, p < 0.001), riboflavin (OR = 0.10; 95 per cent CI = 0.02, 0.39, p < 0.001), pyridoxine (OR = 0.03; 95 per cent CI =0.01, 0.32, p < 0.001), folate (OR = 0.01; 95 per cent CI = 0.00, 0.01, p < 0.001), cobalamine (OR = 0.01; 95 per cent CI = 0.00, 0.01, p < 0.001) , selenium (OR = 0.79 ; 95 per cent CI =1.36, 3.32, p < 0.001), iron (OR =0.68; 95 per cent CI = 0.24, 0.94, p < 0.001) and iodine (OR = 0.36; 95 per cent CI =1.10, 1.38, p < 0.001) had a protective effect on the incidence of PPD. Furthermore, higher intake of vitamin A and beta-cryptoxanthin can increase the incidence of PPD (OR =114.29; 95 per cent CI =17.85, 118.12, p < 0.001) and (OR = 4.85; 95 per cent CI = 1.49, 15.69, p = 0.015), respectively. Originality/value PPD may have destructive effects on the relationship between mother and infant. Results of previous studies demonstrated nutrients are required for the synthesis of neurotransmitters and have biochemical role in the nervous system.
Radiotherapy may improve survival of ES-SCLC with distant metastasis only for patients with one metastatic site: A population-based study

The aim of the present study was to investigate the prognostic impact of RT on patients with extensive stage small cell lung cancer (ES-SCLC) and distant metastasis. Using the Surveillance Epidemiology and End Results (SEER) database, 8,595 patients with ES-SCLC exhibiting distant metastasis treated between 2010 and 2013 were identified. Patient baseline characteristics were compared using the χ2 test. The Kaplan-Meier test was used to analyze subgroup cancer-specific survival (CSS) rate, and differences were compared using a log-rank test. Univariate and multivariate Cox regression models were used to analyze the prognostic variables on CSS. RT was determined to be an independent prognostic factor for patient CSS (P<0.001). In addition, RT could improve the CSS of patients with ES-SCLC with one metastatic lesion (hazard ratio, 0.63; 95% confidence interval, 0.59–0.68; P<0.001), including the bone, brain, liver and lung metastatic sites. However, for patients with two metastatic sites, RT did not improve CSS regardless of metastasis pattern (all P>0.05). To conclude, RT may improve the survival rate of patients with ES-SCLC with distant metastasis, particularly in those with only one metastatic site.
To assess clinical features in patients with CKD- A clinical study

Background: Chronic kidney disease (CKD) encompasses a spectrum of different pathophysiological processes associated with abnormal kidney function and a progressive decline in glomerular filtration rate. The present study was conducted to assess clinical features in patients with CKD. Materials and Methods: The present study was conducted on 64 CKD patients of both genders. Detailed history and clinical examination of the patients was done. Clinical features were recorded. USG was done in all patients. Results: Out of 64 patients, males were 40 and females were 24. Common features were nausea in 49, vomiting in 32, anorexia in 30, bodyache in 46, Dyspnea in 25, diarrhea in 12, hiccups in 35, itching in 38 and unconsciousness in 7. The difference was significant ( p< 0.05). The mean blood urea level was 246.5 mg/dl, serum creatinine was 16.8 mg/dl, hemoglobin was 7.2 g/dl, sodium level was 147.7 mmol/l, potassium level was 5.2 mmol/l, uric acid was 9.6 mg/dl. The difference was significant ( p< 0.05). Conclusion: Raised sodium level raised potassium level, reduced hemoglobin level in patients with CKD. Common finding was nausea, vomiting, anorexia, bodyache, dyspnea, diarrhea, hiccups and itching.
Morphology of the Roots and Canals of Mandibular Third Molars, Their Symmetry and Related Factors Using Cone-Beam Computed Tomography

Introduction: Adequate knowledge about the anatomy of the roots and canals of mandibular third molars is imperative for a successful treatment. This study aimed to use cone-beam computed tomography (CBCT) to evaluate the morphology of the roots and canals of mandibular third molars and their symmetry. Methods and Materials: Totally, 110 CBCT images of bilateral mandibular third molars were evaluated in terms of the number of roots and canals, canal type, isthmus location, location of accessory canals, degree of root curvature and its direction, age and sex of patients and the symmetry of variables in the right and left sides of the mandible. The descriptive statistics, t-test and chi-square test were applied to analyze data. Results: Of all, 71.36% of mandibular third molars had two roots, 20.9% had one single root and 7.72% had three roots. The distal root had one single canal in 89.08%. The mesial root had one single canal in 44.25% and two canals in 49.42%. The Vertucci’s types I (45.40%) and IV (34.48%) had the highest frequency in the mesial root while type I (91.95%) had the highest frequency in the distal root. The deviation of mesial root was towards the distal while distal roots were mainly straight. Conclusion: The symmetry between the right and left mandibular third molars was significantly high. In case of encountering anatomical complexities in dental treatment of a mandibular third molar, dental clinicians should consider the high possibility of presence of the same condition in the contralateral mandibular third molar.
The effects of melatonin administration in different times of day on the brown adipose tissue in rats with high-calorie diet-induced obesity

The aim of our study was to determine morpho-functional state (area of nucleus, brown adipocytes and also area and number of lipid droplets in each cells, general optical density of tissue) of brown adipose tissue in rats with high-calorie (high fat) dietinduced obesity after melatonin administration in different time of the day (morning and evening). Melatonin was administered daily by gavage for 7 weeks in dose 30 mg/kg either 1 h after lights-on (ZT01) or 1 h before lights-off (ZT11) rats with high-calorie diet (HCD). Besides morphometric parameters as well were measured related visceral fat weight and related brown adipose tissue mass. Rats with HCD had huge changes in brown adipocytes morphology, which summarized in become resembles of classical white adipocytes: grown lipid droplets and cells area, but goes down lipid droplets number and optical density of brown adipose tissue. In general brown adipose tissue with above mentioned characteristic from HCD rats lose their ability to conduct strongly thermoproduction function. After melatonin used in rats with HCD arise leveling of pathological changes, which associated with consumption of HCD. Namely, in groups HCD ZT01 and HCD ZT11 we obtain decreased cells and lipid droplets area, increased lipid droplets number and optical density of brown adipose tissue, in relation to group HCD. Therese received changes has evidence about functionally active brown adipose tissue state, which can also dissipate of exceed energy (lipids – triacylglycerols) amount into whole organism during heat production for avoid to its storage in white adipose tissue and in outside adipose tissue. In addition, evening administration of melatonin (group HCD ZT11) demonstrate more activated state of brown adipose tissueand also related visceral weight gain less, than morning(group HCD ZT01). In conclusions, melatonin influence on morpho-functional state brown adipose tissue in rats with HCD, moreover evening administration can use for obesity therapy via its strong action on activate brown adipocytes.