Miracle-dz/UniFAR-FTUs huggingface dataset:
The Effects of Valsartan on Cardiac Function and Pro-Oxidative Parameters in the Streptozotocin-Induced Diabetic Rat Heart

Diabetes mellitus is a major risk factor for cardiovascular diseases, while cardiovascular diseases are a leading cause of morbidity and mortality worldwide. The renin–angiotensin– aldosterone system controls renal, cardiovascular, adrenal function and regulates fluid and electrolyte balance as well as blood pressure. Because of his role, inhibition of reninangiotensin-aldosteron system is another therapy approach that reduces the risk of diabetes and cardiovascular disease. In this study, our goal was to evaluate effect of valsartan,as inhibitor of angiotensin II receptor type 1, on cardiac tissue and function, with focus on cardiodynamic and oxidative stress. The present study was carried out on 20 adult male Wistar albino rats (8 week old and with body masses of 180-200 g). Rats were divided randomly into 2 groups (10 animals per group). Healthy animals treated with 1 μM of valsartan and streptozotocin-induced diabetic animals perfused with 1 μM of valsartan 4 weeks after the induction of diabetes. Our results demonstrated that acute application of valsartan has different effect on cardiodynamics in rat heart of diabetic and healthy animals but did not improve cardiac function in hyperglycemia-induced changes. A challenge for further investigations are studies with chronic or acute administration, alone or in combination with other angiotensin-converting-enzyme inhibitor in various models of diabetes.
Caracterisation des proteines de granules denses de toxoplasma gondii : Etude des interactions protéiques et lipidiques et du rôle des hélices alpha-amphipatiques de GRA2

At the interface with the host cell compartment, the parasitophorous vacuole of the intracellular parasite Toxoplasma gondii comprises several membranous systems, including the membranous nanotubular network formed upon secretion of the dense granule protein GRA2. Using biochemical approaches, I have shown that the GRA proteins form oligomeric complexes, which could explain the solubility of these membrane proteins within the dense granules and after secretion into the vacuolar soluble fraction. Using molecular and cellular approaches, I have investigated the involvement of each of the three GRA2 amphipathic alpha-helices in post-secretory membrane association of the protein, likely via association to phosphatidylinositol phosphates, and in the GRA2 induced-formation of the membranous nanotubular network. These studies led us to propose models for the interaction of GRA2 with membranes and for the tubulation of the membranous nanotubular network.
Caring for the bearded dragon

The Bearded Dragon (Pogona spp), a native of Australia, is now found worldwide as a popular pet. Anecdotal reports indicate that it is starting to surpass the Iguana as the preferred pet reptile in the United States. In Australia, it is found widely distributed in the wild, although strict wildlife regulations mean that it is relatively uncommonly kept as a pet. It is a member of the family Agamidae – the dragon lizards - a group of lizards found throughout the western Pacific, Australia, Indo-Malaysia, Asia and Africa. Characterized by a lack of autotomy (tail loss), the presence of tubercles and spines on juxtaposed scaled skin and a broad, flat fleshy tongue, this family is represented in Australia by the Bearded Dragon, water dragons, the Frilled Lizard and many other species. Most agamids are terrestrial, although a few are semiarboreal. They are oviparous, usually laying their eggs in shallow burrows.
Récepteurs cutanés à la mélanocortine de type 1 (MC1R) et réponses oxydatives aux UVA dans des kératinocytes humains HaCaT

Les ultraviolets A (UVA) sont carcinogenes et produisent des especes reactives de l'oxygene (ERO). Le recepteur a la melanocortine de type 1 (MC1R) est un recepteur couple aux proteines G (RCPG) qui est implique dans la melanogenese et dans l'inflammation cutanee. Certains variants du gene sont associes a un risque accru de melanomes et de carcinomes cutanes. Le MC1R est exprime surtout dans les melanocytes mais son expression peut etre induite par les UV in vitro dans les keratinocytes et in vivo dans la peau. Le recepteur MC1R est active par l'α-MSH. L'objectif de ce travail de these a ete d'etudier les effets du recepteur MC1R sur le stress oxydatif induit par les UVA dans des lignees keratinocytaires humaines HaCaT exprimant le recepteur MC1R ou son variant non fonctionnel Arg151Cys. Nous avons montre que la production d'ERO intracellulaire induite par les UVA est fortement inhibee dans les cellules HaCaT-MC1R et que cette inhibition est renforcee en presence d'α-MSH. L'inhibition du stress oxydatif induit par les UVA dans les cellules transfectees par le MC1R est en partie dependante de la phosphorylation de la sous-unite activatrice, NoxA1 de la NADPH oxydase. Le traitement des cellules HaCaT-MC1R par un inhibiteur du recepteur au facteur de croissance epidermique (EGFR) restaure l'habilite de ces cellules a induire un stress oxydatif apres irradiation UVA. Ces resultats montrent que l'activite constitutive du recepteur MC1R dans des keratinocytes pourrait inhiber le stress oxydatif induit par les UVA via des mecanismes dependants de l'AMPc et de l'EGFR.
Efectos de los Mohos Presentes en Uvas Cosechadas Tardíamente sobre la Calidad de Los Mostos y Vinos Cabernet Sauvignon

Wine grapes (Vitis vinifera L.) have been historically harvest according to their soluble solid contents, (>22.5%) and tartaric acidity (6-7 g·L-1). However, the phenolic content has been proposed and used lately as a better index to determine harvest date. This has implied to delay harvest significantly which has favoried the development of molds that can affect must and wine qualities. The mold incidence found in 13 Cabernet sauvignon vineyards at harvest varied from 4.7 to 66.7%, and were identified as olive green molds (Cladosporium herbarum, Alternaria alternata, Ulocladium sp.), gray mold (Botrytis cinerea), dark brown mold (Aspergillus niger), and Phoma sp. These fungi species, with the exception of B. cinerea, were not necessarily associated to rotten berries. C. herbarum and A. alternata were the most frequently species, found in 11 and 7 of 13 sample sites studied, respectively. Weight of moldy grapes was significantly lower than weight of apparently healthy grapes (p 22,5 %) y la acidez total (6-7 g·L-1), expresada como acido tartarico. Sin embargo, en los ultimos anos se ha privilegiado la madurez fenolica como criterio de cosecha. Esta situacion ha determinado postponer considerablemente la fecha de cosecha lo que ha favorecido el desarrollo de mohos superficiales en las bayas al momento de vinificar. Los mohos identificados en uvas Cabernet sauvignon provenientes de 13 vinedos comerciales a la cosecha correspondieron a mohos verde olivaceos (Cladosporium herbarum, Alternaria alternata, Ulocladium sp.), moho gris (Botrytis cinerea), moho negro (Aspergillus niger) y Phoma sp. Con la excepcion de B. cinerea, estos hongos no necesariamente estuvieron asociados a pudriciones. Cladosporium herbarum y A. alternata fueron las especies mas frecuentes, las que se identificaron en 11 y 7 de 13 muestras de uvas Cabernet sauvignon, obtenidas en vinedos comerciales a la cosecha. En promedio, la perdida de peso se estimo en 32,8%, siendo siempre significativamente (p<0,05) inferior el peso de las uvas mohosas. El vino obtenido con uvas mohosas presento menor intensidad colorante, un matiz mas amarillo, menor contenido de antocianos y de polifenoles totales. A su vez disminuyo el contenido de anhidrido sulfuroso libre y aumento el anhidrido sulfuroso combinado. Ademas la fermentacion alcoholica se retardo en al menos 12 dias en relacion con la fermentacion de un mosto con uvas aparentemente sanas cosechadas en el mismo vinedo. En consecuencia, se concluye que la alta incidencia de mohos superficiales presentes en las uvas al momento de la cosecha afecta negativamente el peso de la cosecha y las cualidades sensoriales de los vinos obtenidos, particularmente el color, olor y sabor. La tendencia generalizada de la industria chilena a cosechar tardiamente en busca de una mejor calidad del vino resultante no necesariamente permitiria cumplir con este objetivo.
Telestroke: Overcoming Barriers to Lifesaving Treatment in Rural Hospitals

Telestroke is the use of telemedicine specifically for stroke care. In the United States, about 40 neurologists are available per 100,000 persons. Acute ischemic stroke is often misdiagnosed by primary care or emergency medicine physicians in up to 30 percent of cases. A key advantage of using telestroke is the ability to treat patients in an efficient manner in rural areas where neurologists may not always be readily accessible. The purpose of this research study was to assess the main barriers of telestroke network implementation in rural hospitals. The methodology applied was a systematic literature review with a total of 75 sources used. Significant barriers exist preventing widespread success and adoption of telestroke, including high upfront capital expenditures, insufficient reimbursement by payers, and regulatory hurdles. While the telestroke referral network adds a significant financial burden on hospitals through infrastructure costs such as equipment purchases, staffing, training, and maintenance of the network, it can offer cost savings from the hospital’s point of view while also improving patient outcomes. From a regulatory perspective, the use of telestroke has been tied to telehealth regulations and may not be well defined in all areas in terms of medical board rules, national standards, or clarifications of malpractice surrounding the use of this technology.
Body Mass Index, Health Related Behaviors and Obesity among Female Adolescent Students

Obesity is a worldwide public health problem affecting all levels and age groups within the society. Health-related behaviors can negatively affect adolescents' health which contributes to the main causes of morbidity and mortality in this age group. Early identification of health risk behaviors during adolescents are considered to be an important element in the prevention of obesity. Aim: to identify the relationship between body mass index (BMI), health related behaviors and obesity among female adolescent students. Design: a descriptive design was used . Setting: The study was conducted at Technical Institute of Nursing, Ain Shams University. Sample: Simple random sample of 100 female adolescent students from the previously mentioned setting Tools of data collection: included a structured interview questionnaire to assess student's body mass index and health behaviors related to obesity Results: The results showed that less than half of students were normal weight (47%) compared to 39% for overweight and obese and 14% for underweight. The most common health problems as reported by the students were snoring during sleep 14%, anemia 13%, back & knee pain 11%, headache and high blood pressure 10%, diabetes and bronchial asthma 6%. Adolescents had some unhealthy behaviors regarding eating pattern, technology usage, exercise programs. There is a significant correlation between students' body mass index and their eating pattern. As regards students' body mass index and their technology usage, television watching was significantly associated with overweight and obese students than normal weight and underweight students. It was clear that the majority of participants reporting that they use internet more than 3 hours per day and they eat while using the internet. Only 19%of overweight and 16.7% of obese students have exercise program in compared to 57.1% of underweight and 72.3% of normal weight students instead of the majority of the sample did not have any barriers to regular exercise. Conclusion. The study concluded that, there was a strong relationship between body mass index, health related behaviors and obesity among female adolescent students. A high prevalence of them had unhealthy behaviors related to dietary pattern, usage of technology, exercise programs. Recommendations: The study recommended that there is a need for application of educational programs regarding healthy behaviors to reduce the tendency of overweight and obesity among adolescent students.
Chemical Structure and Functional Organization of lac Repressor from Escherichia coli

INTRODUCTION Jacob and Monod (1961) proposed in their operon model that control genes would make repressors which would turn off the structural genes. This was an act of turning away from the hitherto discussed instructive theories of control. The essential and also new idea put forward was that the repressor acts as an intermediate to make the connection between the signal provided by the inducer or corepressor molecules and the target for the control, the operator. The signal molecules bind to the repressor and alter its affinity for the operator. If we want to understand these interactions, we have to answer the question about the molecular nature and chemical structure of both elements. The isolations of nonsense mutations in the λ repressor C I gene (Jacob et al. 1962; Thomas and Lambert 1962) and in the lac repressor I gene (Bourgeois et al. 1965; Muller-Hill 1966) provided the first convincing genetic evidence for the nature of repressors. Suppression restored repressor activity and showed that the repressor genes encode proteins. The final proof was brought forth by Gilbert and Muller-Hill (1966) with the isolation of the lac repressor. They demonstrated that the repressor is a protein which binds β -D-galactosides in vitro and cosediments with lac operator DNA only in the absence of the gratuitous inducer isopropyl-1-thio- β -D-galactopyranoside (IPTG) (Gilbert and Muller-Hill 1966Gilbert and Muller-Hill 1967). The amount of repressor made by the haploid wild-type cell is very small. The intracellular concentration of lac repressor was estimated by Gilbert and Muller-Hill (1966) to be on the.
Isolation and identification of Fusarium species associated with Fusarium ear rot disease of corn.

Fusarium species associated with corn (Zea mays) are very diverse and widely distributed throughout Malaysia. Out of 657 samples cultured, a total of 220 Fusarium isolates were obtained from corn plants showing the typical symptoms of Fusarium ear rot in 12 locations throughout Malaysia. All the isolates were identified into 10 species based on the morphological characteristics that emphasized on growth rates, colony features and microscopic characteristics. A total of 117 Fusarium isolates were classified into four species in the section Liseola and their allied, tentatively identified as F. proliferatum (58), F. subglutinans (34), F. verticillioides (24), and F nygamai (1). Meanwhile, F. proliferatum was the most prevalent species in all the sampling areas. 103 isolates, which were classified into six other Fusarium species belonging to different sections, were also isolated and identified, and these included F semitectum (47), F oxysporum (20), F. pseudograminearum (19), F solani (15), F equiseti (1), and F. longipes (1). F semitectum was the highest among other common saprophytic fungi in corn. F pseudograminearum was only isolated from the samples obtained from Cameron Highlands, Pahang. In term of species diversity, Fusarium species was the highest obtained in Semenyih, Selangor, with H'=1.72.
FE(II) OXIDATION BY ANAEROBIC PHOTOTROPHIC BACTERIA: MOLECULAR MECHANISMS AND GEOLOGICAL IMPLICATIONS

In this thesis, the hypothesis that photoautotrophic Fe(II)-oxidizing bacteria catalyzed the deposition of Banded Iron Formations (BIFs), an enigmatic class of ancient sedimentary rocks is explored. Ecophysiological, geochemical, genetic and biochemical approaches are taken to elucidate the molecular mechanism of photoautotrophic Fe(II) oxidation in an effort to identify molecular biosignatures that are unique to this metabolism and capable of being preserved BIFs. In an ecophysiological approach, we show that Fe(II) oxidation by these phototrophs proceeds at appreciable rates in the presence of high concentrations of H2 when CO2 is abundant. These findings substantiate a role for the involvement of these phototrophs in BIF deposition under the presumed geochemical conditions of the Archean. In a geochemical approach, we find that although phylogenetically distinct phototrophs fractionate Fe isotopes in a way that is consistent with Fe isotopic values found in Precambrian BIFs, it is unlikely that this fractionation can be used as a biosignature for this metabolism given its similarity to fractionations produced by abiotic Fe(II) oxidation reactions. In two distinct genetic approaches, we identify genes involved in Fe(II) oxidation in Rhodopseudomonas palustris TIE-1 and Rhodobacter SW2. Genes identified in TIE-1 encode a predicted integral membrane protein that appears to be part of an ABC transport system and a putative CobS, an enzyme involved in cobalamin (vitamin B12) biosynthesis. Candidate genes on a cloned fragment of the Rhodobacter SW2 genome that confer Fe(II) oxidation activity to a non-oxidizing strain include those predicted to encode permeases and a protein with potential redox capability. Finally, in a preliminary biochemical approach, c-type cytochromes and other proteins that are exclusive or more highly expressed under Fe(II) growth conditions in TIE-1 and SW2 are identified in SDS-PAGE gels. The work described here furthers our search for a biosignature unique to photoautotrophic Fe(II) oxidation by providing mechanistic information on this metabolism.
A photographic evaluation of factors prevalent in Nepalese smile: A cross-sectional study

Aim: The aim of this study was to find out the prevalence of different characteristics of smile and to determine the level of normalcy based on the above. Materials and Methods: The standardized digital photographs of 529 participants were collected and transferred to personal computer in which the different characteristics of smile were visually inspected. Results: Smile with average upper lip position (54%) straight upper lip curvature in smile (46.9%), parallel incisal line to upper border of lower lip (51.8%) and incisal edges of maxillary dentition not touching to lower lip (62.4%) were most occurring characteristics with statistically significant prevalence of gingival and mucosal smiles amongst females. Conclusions: The study outlined few most occurring characteristics in smile of Nepalese population. The average upper lip position, straight upper lip curvature and parallel incisal line were some of the most prevalent characteristics in Nepalese smile.
Kinematic Analysis of the Musculoskeletal System Diabetic Type I

This study aimed to apply the principles of biomechanics science correctly, also to be is nested in the treatment of diabetics, and establish kinematic record for musculoskeletal diabetics. In order to improve the quality of life and care for diabetics. To achieve this, the researchers used the kinematic analysis of the musculoskeletal system for (8) mail patients with type1 diabetes, purposive selected and belonging to Jordanian team of Diabetes (age: 21±0.7years, height: 164± 4 cm, mas:58± 0.2 kg, the experience of the disease, 13.3±0.8 years). The study sample was filmed by using video camera (Sony HDR-CX220E) reached speed (50) frame/s. The study results showed that musculoskeletal system for Diabetic Type I differences in their shape of musculoskeletal the rest of the population, consequently people with Type 1 Diabetes have a swayback posture and a lower chest forward posture. and the average of sample study in the flexion spine(77)° and (15)°for extension spine. Where it formed 81% from natural flexion spine and 45% from natural extension spine. The researchers recommend: the necessity informed Diabetic Type I on kinematic analysis of the musculoskeletal system.
Recirculation of innate lymphocyte subsets in the skin

The trafficking of innate-like lymphocytes, such as γδ T cells and B-1 B cells, has garnered comparatively little attention from the immunological community relative to conventional T and B cells. However, recent studies have shown that innate-like cell subsets are critical for immune regulation and host defense. In this study, we use a classic ovine lymph cannulation model to describe the phenotype and function of γδ T cells migrating through the skin. We find that γδ T cells traveling in the skin-draining afferent lymph are IFN-γand/or IL-17-producing effector cells that express high levels of the skinand inflammation-seeking molecule Eselectin ligand. Notably, they also lack expression of CCR7, indicating that they use alternative receptors for egress. Next, we analyze B cell subset composition, repertoire, and trafficking in the skin of sheep in the lymphatic cannulation model. We find a heterogeneous population of B cells in the skin and skin-draining lymph increases in inflammation that contains a subset of B-1-like B cells coexpressing IgM and CD11b. Furthermore, we show that skin accumulation of B cells and antibody-secreting cells during inflammation increases local antibody titers, which may augment host defense and autoimmunity. We then extend our findings of cutaneous B-1 B cells to the mouse, analyzing both uninflamed skin and skin with chronic inflammation from complete Freund's adjuvant, well as human tissue. We find that B-1 B cells, unlike conventional follicular B-2 B cells, efficiently enter into the inflamed skin and differentially express the trafficking molecule α4β1 integrin (VLA-4), which facilitates their entry. Furthermore, innate B cells are a contributing source of cutaneous IL-10 in both IL-10-reporter mice and normal human skin. These findings, initiated in the sheep model then followed up and supported by experiments in mice and human tissues, demonstrate the evolutionary similarity between mammalian species. They also validate the utilization of multiple models to allow for experimental setups not possible in all species. More importantly, the further characterization of γδ T cells and the new description of skin B and B-1 cells uncovers additional targets for regulating the cellular composition of a cutaneous immune response. In summary, the data support a model in which innate-like lymphocytes are poised to migrate into barrier sites, including the skin, where they rapidly provide requisite effector functions, such as cytokine and/or antibody production, and fulfill an emerging role in skin immunity. Degree Type Dissertation Degree Name Doctor of Philosophy (PhD) Graduate Group Cell & Molecular Biology First Advisor Gudrun F. Debes