Miracle-dz/UniFAR-FTUs huggingface dataset:
RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation

Cardiac arrest (CA) is the leading cause of death around the world. Survivors after CA and cardiopulmonary resuscitation (CPR) develop moderate to severe cognitive impairment up to 60% at 3 months. Accumulating evidence demonstrated that long non-coding RNAs (lncRNAs) played a pivotal role in ischemic brain injury. This study aimed to identify potential key lncRNAs associated with early cognitive deficits after CA/CPR. LncRNA and mRNA expression profiles of the hippocampus in CA/CPR or sham group were analyzed via high-throughput RNA sequencing, which exhibited 1920 lncRNAs and 1162 mRNAs were differentially expressed. These differentially expressed genes were confirmed to be primarily associated with inflammatory or apoptotic signaling pathways through GO and KEGG pathway enrichment analysis and coding-noncoding co-expression network analysis. Among which, five key pairs of lncRNA-mRNA were further analyzed by qRT-PCR and western blot. We found that the lncRNANONMMUT113601.1 and mRNA Shc1, an inflammation and apoptosis-associated gene, exhibited the most significant changes in hippocampus of CA/CPR mice. Furthermore, we found that the correlations between this lncRNA and mRNA mainly happened in neurons of hippocampus by in situ hybridization. These results suggested that the critical pairs of lncRNA-mRNA may act as essential regulators in early cognitive deficits after resuscitation.
Sense of Purpose in Life and Likelihood of Future Illicit Drug Use or Prescription Medication Misuse

Supplemental digital content is available in the text. ABSTRACT Objective In the United States, 28.6 million people used illicit drugs or misused prescription drugs in the last 30 days. Thus, identifying factors linked with lower likelihood of future drug misuse is an important target for research and practice. Sense of purpose in life has been linked with better behavioral and physical health outcomes. Furthermore, a higher sense of purpose may reduce the likelihood of drug misuse because it has been linked with several protective factors including enhanced ability to handle stress, higher pain tolerance, and lower impulsivity. However, the association between sense of purpose and drug misuse has been understudied. Thus, we tested whether people with a higher sense of purpose at baseline had a lower likelihood of future drug misuse 9 to 10 years later. Methods This study included 3535 middle-aged adults from the Midlife in the United States Study who were not misusing drugs at baseline. Using multiple logistic regression models, we assessed whether baseline purpose in life was associated with risk of misusing drugs 9 to 10 years later. Results Among respondents not misusing drugs at baseline, people in the highest quartile of purpose (versus lowest quartile) had a substantially lower likelihood of future drug misuse in a model adjusting for demographic variables (odds ratio = 0.50, 95% confidence interval = 0.31–0.83). Associations remained evident after additionally adjusting for psychological distress, baseline health, and health behaviors. Conclusions A growing knowledge base suggests that a sense of purpose can be increased. Additional research is needed to evaluate sense of purpose as a novel target in the prevention and reduction of drug misuse.
Visual cues used in the choice of mate by fowl and their potential importance for the breeder industry

Modern fowl breeder production has resulted in a wide disparity between the environment, mating behaviour and morphology of modern breeds in comparison with progenitor species. The environment is strictly controlled in most respects and dim lighting is maintained with luminaires that were originally designed for humans and that differ substantially in terms of intensity and colour balance from natural daylight. The elaborate mating displays observed in feral poultry and red jungle fowl rarely, if ever, occur in breeder houses. In most avian species females choose the males, whereas cockerels in breeder houses often appear to mate forcibly and aggressively with hens in the absence of any assessment by the hen. Cockerel and hen breeder fowl also differ morphologically from their progenitor species through genetic selection (larger and heavier) or mutilations (dubbing, etc). Given the apparently large differences between the subtle, gradually evolved mating ‘ecology’ found naturally and the production environments found commercially, it is perhaps surprising that little consideration has been given to this disparity in modern breeder production environments. An understanding of the factors governing choice of mate in commercial breeder houses and the influence of the visual environment may highlight novel methods whereby efficiency of these enterprises can be improved and elements of the welfare of breeder fowl assessed.
Casticin induces apoptosis and mitotic arrest in pancreatic carcinoma PANC-1 cells

Pancreatic cancer is one of the most malignant tumors that responded poorly to currently available chemotherapy. Casticin, a flavonoid compound, has been reported to induce apoptosis in various cancer cell lines. However, there is no report on the anti-pancreatic cancer potential of casticin. In the present study, we showed for the first time that casticin strongly inhibits the growth of PANC-1 pancreatic carcinoma cells. The anti-proliferative effect assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5Diphenyltetrazolium Bromide (MTT) assay demonstrated that casticin inhibited the growth of PANC-1 cells in a dose-dependent manner. Further analysis using flow cytometry and immunoblot showed that the growth inhibitory effect of casticin was associated with cell cycle arrest at G2/M phase and induction of apoptosis. Mechanistic studies indicated that the induction of apoptosis was associated with up-regulation of pro-apoptotic protein Bax, down-regulation of anti-apoptotice protein Bcl-2 and activation of caspase-3. These findings suggest that casticin may be a promising candidate for treating human pancreatic cancer.
Risk Factors Associated With Early and Late Posttraumatic Multiorgan Failure. An Analysis from Retrauci.

OBJECTIVE To analyse factors associated with the development of early and late multiorgan failure (MOF) in trauma patients admitted to the intensive care unit (ICU). METHODS Spanish Trauma ICU Registry (RETRAUCI). Data collected from 52 trauma ICU between March 2015 and December 2019. We analysed the incidence, outcomes and the risk factors associated with early (< 72 h) or late (beyond 72 h) MOF in trauma ICU patients. Multiple logistic regression analysis was performed to analyse associated factors. RESULTS After excluding patients with incomplete data, 9598 trauma ICU patients constituted the study population. Up to 965 patients (10.1%) presented with MOF, distributed by early MOF in 780 patients (8.1%) and late MOF in 185 patients (1.9%). The multivariate analysis showed that early MOF was associated with: ISS ≥ 16 (OR 2.80), hemodynamic instability (OR from 2.03 to 43.05), trauma-associated coagulopathy (OR 2.32) and acute kidney injury (OR 4.10). Late MOF was associated with: age > 65 years (OR 1.52), hemodynamic instability (OR from 1.92 to 9.94), acute kidney injury (OR 4.22) and nosocomial infection (OR 17.23). MOF was closely related to mortality (crude OR (95%CI) 4.77 (4.22-5.40)). CONCLUSIONS Multiorgan failure was recorded in 10% of trauma ICU patients, with early MOF being the predominant form. Early and late MOF forms were associated with different risk factors, suggesting different pathophysiological pathways. Early MOF was associated with higher severity of injury and severe bleeding-related complications and late MOF with advanced age and nosocomial infection.
Increased platelet distribution width and reduced IL-2 and IL-12 are associated with thrombocytopenia in Plasmodium vivax malaria

BACKGROUND Thrombocytopenia in malaria involves platelet destruction and consumption; however, the cellular response underlying this phenomenon has still not been elucidated. OBJECTIVE To find associations between platelet indices and unbalanced Th1/Th2/Th17 cytokines as a response to thrombocytopenia in Plasmodium vivax infected (Pv-MAL) patients. METHODS Platelet counts and quantification of Th1/Th2/Th17 cytokine levels were compared in 77 patients with uncomplicated P. vivax malaria and 37 healthy donors from the same area (endemic control group - ENCG). FINDINGS Thrombocytopenia was the main manifestation in 55 patients, but was not associated with parasitaemia. The Pv-MAL patients showed increases in the mean platelet volume (MPV), which may be consistent with larger or megaplatelets. Contrary to the findings regarding the endemic control group, MPV and platelet distribution width (PDW) did not show an inverse correlation, due the increase in the heterogeneity of platelet width. In addition, the Pv-MAL patients presented increased IL-1β and reduced IL-12p70 and IL-2 serum concentrations. Furthermore, the reduction of these cytokines was associated with PDW values. MAIN CONCLUSIONS Our data demonstrate that an increase in MPV and the association between reductions of IL-2 and IL-12 and PDW values may be an immune response to thrombocytopenia in uncomplicated P. vivax malaria.
Human papillomavirus vaccine timing associated with eventual human papillomavirus diagnosis in women

Nearly all cases of cervical cancer are attributable to human papillomavirus (HPV), making it a significant women’s health issue. Though there have been advances in the prevention of HPV via vaccination, significant barriers continue to suppress vaccination rates for girls. Delaying vaccination until after sexual debut increases a woman’s chance of HPV infection, but there has been no quantification of this risk in the literature. The present study sought to address this gap via secondary data analysis with 173 female participants from the 2015–2016 National Health and Nutrition Examination Survey dataset. Results indicate that women in the sample who received the HPV vaccine after their sexual debut were 2.63 times more likely than women who receive the vaccine before their sexual debut to report an HPV diagnosis. These results have clear public and sexual health implications.
Detecting Common Eye Diseases Using the First Teleophthalmology GlobeChek Kiosk in the United States: A Pilot Study.

PURPOSE The aim of this study was to assess the benefit and feasibility of the teleophthalmology GlobeChek kiosk in a community-based program. DESIGN Single-site, nonrandomized, cross-sectional, teleophthalmologic study. METHODS Participants underwent comprehensive evaluation that consists of a questionnaire form, brief systemic evaluation, screening visual field (VF), and GlobeChek kiosk screening, which included but not limited to intraocular pressure, pachymetry, anterior segment optical coherence tomography, posterior segment optical coherence tomography, and nonmydriatic fundus photography. The results were evaluated by a store-and-forward mechanism and follow-up questionnaires were obtained through phone calls. RESULTS A total of 326 participatents were screened over 4 months. One hundred thirty-three (40.79%) participants had 1 condition in either eye, and 47 (14.41%) had >1 disease. Seventy (21.47%) had glaucoma, 37 (11.34%) narrow-angles, 6 (1.84%) diabetic retinopathy, 4 (1.22%) macular degeneration, and 43 (13.10%) had other eye disease findings. Age >65, history of high blood pressure, diabetes mellitus, not having a dental examination >5 years, hemoglobn A1c measurement of ≥5.6, predibates risk score of ≥9, stage 2 hypertension, and low blood pressure were found to be significant risk factors. As for the ocular parameters, all but central corneal thickness, including an intraocular pressure >21 mm Hg, vertical cup to disc ratio >0.7, visual field abnormalities, and retinal nerve fiber layer thinning were found to be significant. CONCLUSIONS GlobeChek kiosk is both workable and effective in increasing access to care and identifying the most common causes of blindness and their risk factors.
A Large-Scale European Union Study of Aircrew Fatigue During Long Night and Disruptive Duties.

INTRODUCTION: We examined aircrew fatigue during the following flight duty periods (FDPs) mentioned in the European Union (EU) Flight Time Limitations (FTLs): night FDPs longer than 10 h and FDPs typical of disruptive schedules (early starts, late finishes, and nights). An alternative way of classifying night FDPs was also examined to reveal possible subcategories that warrant special attention.METHODS: A total of 392 aircrew members (96 women) representing 24 airlines participated in the study. Their FDPs were measured by a diary, sleep by the diary and wrist-actigraphy, and fatigue by the Karolinska Sleepiness Scale (KSS) over 14 consecutive days. The KSS ratings given at top of descent (TOD) served as the main outcome.RESULTS: The probability of high fatigue (KSS ≥ 7) at TOD was 0.41 and 0.32 during long (>10 h) and short night (≤10 h) FDPs, respectively. The corresponding value was 0.19 for early starts, 0.31 for late finishes, 0.34 for night FDPs, and 0.15 for day FDPs (reference). The main predictors of high fatigue were FDP's encroachment on the window of circadian low (WOCL, 02:00 h-05:59 h) and prior sleep. Within the night category, FDPs fully covering the WOCL showed the highest probability of high fatigue at TOD (0.42). DISCUSSION: Late finish and night FDPs warrant special attention in fatigue management. Within the night category, the same holds for FDPs that fully cover the WOCL. To manage fatigue, adjustments of the FTLs seem to be a limited strategy and therefore other measures, including maximizing preflight sleep, are needed.Sallinen M, van Dijk H, Aeschbach D, Maij A, Åkerstedt T. A large-scale European Union study of aircrew fatigue during long night and disruptive duties. Aerosp Med Hum Perform. 2020; 91(8):628-635.
T-DNA tagging of a flowering-time gene and improved gene transfer by in planta transformation of Arabidopsis

Gene tagging with insertional mutagens greatly facilitates the isolation of novel genes. A new collection of Arabidopsis T-DNA tag insertion lines (n=2165) was generated by in planta transformation. Whole plants were vacuum-infiltrated in a suspension of Agrobacterium carrying the pGKB5 tagging vector. The efficiency of transformation increased with addition of the surfactant Silwet L-77 (0.005% v/v) to the Agrobacterium suspension. Visual screens of the T-DNA lines identified two mutants with floral defects. Allelism tests suggested that a mutation in the GIGANTEA gene was responsible for the late-flowering phenotype of one of the mutants. Linkage analysis indicated that the GIGANTEA gene was tagged in this mutant.
DETECCIÓN EN MUESTRA CLÍNICA E IDENTIFICACIÓN DE AISLADOS DEL VIRUS DE LA BRONQUITIS INFECCIOSA AVIAR POR UN ENSAYO DE REVERSO TRANSCRIPCIÓN ACOPLADO A REACCIÓN EN CADENA DE LA POLIMERASA

La bronquitis infecciosa aviar (BIA) es una enfermedad viral ampliamente diseminada en el mundo y se considera una de las principales causas de perdidas economicas significativas en la industria avicola, debido a que afecta el desarrollo de aves de engorde y ponedoras. En Cuba, el diagnostico actual se realiza por aislamiento viral y es dificil su identificacion por metodos convencionales. Para identificar la posible presencia del virus de la BIA en aislados cubanos se aplico un ensayo de reverso transcripcion acoplado a reaccion en cadena de la polimerasa (RT-PCR) especifico para este virus. Las muestras de traquea-pulmon fueron tomadas como grupos de tres y cuatro aves despues de la necropsia de cada una, evaluandose un total de once aves. Las secuencias del virus de la BIA fueron detectadas en dos de los tres grupos evaluados. En uno de estos grupos se logro ademas, la deteccion del virus a partir de la muestra clinica; aspecto importante para la diferenciacion con enfermedades graves que cursan con un cuadro clinico similar como la Influenza aviar y la Enfermedad de Newcastle. La RT-PCR mostro potencial para ser usado en la identificacion de aislados del virus de la BIA, asi como en el examen directo a partir de muestras clinicas, lo que aporta posibilidades de su empleo en nuestro pais para la deteccion rapida, sensible y especifica de este agente, aspecto esencial en el diagnostico y control de esta infeccion viral.
Teeth Classification Based on Haar Wavelet Transform and Support Vector Machine

—To improve the efficiency of stomatology practitioners, this paper proposed a novel teeth type classification method. Our method was based on three successful components: Haar wavelet transform, principal component analysis, and support vector machine. We create a 120-image dataset, with 30 images for incisor, canine, premolar, and molar. The results showed our method achieved an overall classification accuracy of 81.83± 1.79%, better than decision tree and multilayer perceptron methods.
Management of fisheries on forage species: the test-bed for ecosystem approaches to fisheries

In the1970s and1980s, core ideas aboutmanagementof fisheries on forage species emerged fromworkon thedynamics of foodwebmodels and multispecies assessments, leading to proposals for management that took some account of the role of forage species in marine ecosystems. Key developments in those years are summarized in the first part of this paper. From the 1980s to the 2000s, studies of the response of forage species to environmental variation brought into question the robustness of management strategies for forage species. As a result, additional management strategies were proposed to accommodate environmental drivers as well as dependent predators. The paper reviews these developments. This paper brings these separate lines together in a systematic framework for evaluating the performance of six different management strategies for forage species, relative to four different ecosystem considerations, as well as relative to the contribution of forage fisheries to economic prosperity and food security. The tabulatedoutcomes synthesize primary and secondary literature and meeting deliberations as the application of an ecosystem approach to management has evolved. No strategy is optional for all forage fisheries. As experience accumulates, the guidance in the tables comprising the framework will improve.
Influence of Heavy Metals on Seed Germination and Early Seedling Growth in Eruca sativa Mill.

Heavy metals present in soil and water naturally or as contaminants from human activities can cause bioaccumulation affecting the entire ecosystem and pose harmful health consequences in all life forms. Some famous non-food hyperaccumulators such as Thlaspi caerulescens, Sedum alfredii, Pteris vittata, Arabidopsis halleri and Athyrium yokoscense are of very little economic value, making it difficult for them to be used for phytoremediation. In this paper, the influence of heavy metals Cu, Ni, Zn, Hg, Cr, Pb and Cd on seed germination and early seedling growth in oil crop Eruca sativa was evaluated under laboratory conditions. Our results indicated that among the 7 heavy metals tested, only Ni at higher concentrations (1 mM and above) significantly decreased the Eruca seed germination in a dose-dependent manner. All heavy metals except Zn and Ni decreased the root length first, then the shoot length or the fresh seedling weight, and seed germination was always the last to be influenced. With Ni, the root length, shoot length and fresh seedling weight were stimulated when Ni concentrations were under 1 mM; with Zn, the root length, shoot length and fresh seedling weight were increased by all concentrations tested (0.20 - 5.0 mM). Our results indicated that Eruca is tolerant or moderately tolerant to Cu, Hg, Cr, and Cd and highly tolerant to Pb, Ni and Zn, and can be developed as an industrial oil crop for phytoremediation of soils contaminated by heavy metals.
Named Data Networking in Vehicular Ad Hoc Networks: State-of-the-Art and Challenges

Information-centric networking (ICN) has been proposed as one of the future Internet architectures. It is poised to address the challenges faced by today’s Internet that include, but not limited to, scalability, addressing, security, and privacy. Furthermore, it also aims at meeting the requirements for new emerging Internet applications. To realize ICN, named data networking (NDN) is one of the recent implementations of ICN that provides a suitable communication approach due to its clean slate design and simple communication model. There are a plethora of applications realized through ICN in different domains where data is the focal point of communication. One such domain is intelligent transportation system realized through vehicular ad hoc network (VANET) where vehicles exchange information and content with each other and with the infrastructure. Up to date, excellent research results have been yielded in the VANET domain aiming at safe, reliable, and infotainment-rich driving experience. However, due to the dynamic topologies, host-centric model, and ephemeral nature of vehicular communication, various challenges are faced by VANET that hinder the realization of successful vehicular networks and adversely affect the data dissemination, content delivery, and user experiences. To fill these gaps, NDN has been extensively used as underlying communication paradigm for VANET. Inspired by the extensive research results in NDN-based VANET, in this paper, we provide a detailed and systematic review of NDN-driven VANET. More precisely, we investigate the role of NDN in VANET and discuss the feasibility of NDN architecture in VANET environment. Subsequently, we cover in detail, NDN-based naming, routing and forwarding, caching, mobility, and security mechanism for VANET. Furthermore, we discuss the existing standards, solutions, and simulation tools used in NDN-based VANET. Finally, we also identify open challenges and issues faced by NDN-driven VANET and highlight future research directions that should be addressed by the research community.
A case of cowpox virus infection in the UK occurring in a domestic cat and transmitted to the adult male owner

DEAR EDITOR, A 43-year-old man presented with a 25 9 20-mm fluctuant, nontender, solitary, haemorrhagic bulla with central necrosis on the left volar wrist, left-arm swelling (shown on days 2, 4 and 8), lymphangitis and pyrexia of 38 5 °C. His cat had recently become unwell and developed several dozen 3–4-mm erythematous annular and eroded lesions. These histologically demonstrated epidermal necrosis and pyogranulomatous dermatitis with large eosinophilic cytoplasmic inclusions within keratinocytes, which were consistent with poxvirus infection. Public Health England (Porton Down, UK) performed polymerase chain reaction of the haemorrhagic bulla fluid from the man, which identified Orthopoxvirus (suspected cowpox). Cats infected by bank voles can transmit the self-limiting disease to humans through minor skin fissures, remaining localized in immunocompetent individuals. The end of compulsory vaccinia vaccination 40 years ago and subsequent reduced cross-protection among orthopoxviruses may result in increased incidence of zoonotic Orthopoxvirus infections. C. Haddadeen, M. Van Ouwerkerk, T. Vicek and A. Fityan Department of Dermatology, University Hospital Southampton NHS Foundation Trust, Southampton, UK; Pet Vet, Veterinary Dermatology, Thornhill, Southampton, UK; and Department of Veterinary Pathology, Finn Pathologists, Harleston, Norfolk, UK Correspondence: Adam Fityan. Email: adam.fityan@uhs.nhs. uk