Miracle-dz/UniFAR-FTUs huggingface dataset:
Differential expression of cell adhesion genes: Implications for drug resistance

It is well known that tumors arising from tissues such as kidney, pancreas, liver and stomach are particularly refractory to treatment. Searching for new anticancer drugs using cells in culture has yielded some effective therapies, but these refractory tumors remain intractable. Studies that compare cells grown in suspension to similar cells grown attached to one another as aggregates have suggested that it is adhesion to the extracellular matrix of the basal membrane that confers resistance to apoptosis and, hence, resistance to cytotoxins. The genes whose expression correlates with poor survival might, therefore, act through such a matrix‐to‐cell suppression of apoptosis. Indeed, correlative mining of gene expression and patient survival databases suggests that poor survival in patients with metastatic cancer correlates highly with tumor expression of a common theme: the genes involved in cell adhesion and the cytoskeleton. If the proteins involved in tethering cells to the extracellular matrix are important in conferring drug resistance, it may be possible to improve chemotherapy by designing drugs that target these proteins. © 2004 Wiley‐Liss, Inc.
Determination of soluble extractives and physico-chemical studies of bark of Sesbania sesban ( L ) Merr

Sesbania sesban (L) Merr. has shown potential as a multipurpose tree in agro-forestry systems. The bark of sesbania sesban has been traditionally used as astringent, in diarrhea, ulcers, bronchitis, tumors, cirrhoses of liver, hypertension & spleen enlargement. This plant has got good medicinal importance [1]. This study was intended to evaluate the physico-chemical and some pharmacognostic parameters of bark of sesbania sesban. The bark powder was studied for proximate analysis & quantitative determination in terms of parameters such as loss on drying, total ash value, acid-insoluble ash value, water-soluble ash values & extractive values in different solvents. The pharmacognostic parameters are useful for setting standards for crude drug and help to differentiate the plant sample from the adulterants. The pharmacognostic constants for the bark of this plant & the numerical standards reported in this work could be useful for the compilation of a suitable monograph for its proper identification.
Major histocompatibility complex class II gene associations with anti-U1 small nuclear ribonucleoprotein antibody. Relationship to immunoreactivity with individual constituent proteins.

OBJECTIVE To better define immunogenetic associations with the anti-U1 ribonucleoprotein (U1 RNP) autoantibody response. METHODS HLA class II alleles were determined by genotyping in 49 Japanese rheumatic disease patients with anti-U1 RNP antibody and 43 race-matched healthy controls. Immunoreactivities to U1 RNP constituent proteins (70K, A, B/B', and C) were detected by immunoblots using purified HeLa cell Sm antigen, and antibody titer was determined by passive hemagglutination assay. RESULTS DQB1*0302 was significantly more frequent in anti-U1 RNP-positive patients than in controls (43% versus 14%; odds ratio [OR] = 4.6, corrected P = 0.03). All anti-U1 RNP-positive patients had either a DQB1*0601, *0602, *0301, *0302, or *0303 allele, which share tyrosine at position 30, and the amino acid sequence Thr, Arg, Ala, Glu, Leu, Asp, and Thr at positions 71-77 in the DQB1 beta 1 domain. In contrast, one of these alleles was found in 81% of the controls (OR = 24, P = 0.002). In addition, anti-U1 RNP antibody was associated with unique DQB1*0302; DRB1*0401 haplotype. Anti-70K reactivity and antibody titer were positively associated with a basic amino acid residue, arginine or histidine, at position 13 (DR2 or DR4) and were negatively associated with the amino acid sequence Ile, Leu, Glu, and Asp at positions 67-70, which was present in some of the DR5-, DR6-, and DR8-associated alleles, in the DRB1 beta 1 domain. Anti-C reactivity was strongly associated with DR2, particularly with DRB1*1502. CONCLUSION The several shared epitopes located on HLA-DRB1 and DQB1 genes control the anti-U1 RNP autoantibody response.
Vascular angular remodeling by kissing-Y stenting in wide necked intracranial bifurcation aneurysms

Introduction Single stent deployment leads to a change in vascular geometry in wide necked bifurcation aneurysms. In some complex cases, the use of the single stent technique might not be sufficient or may not be feasible. The kissing-Y stenting technique appears to be an alternative endovascular treatment option. The aim of this study was to evaluate the effects of the kissing-Y stenting technique on vascular angular remodeling. Methods 21 patients with wide necked intracranial bifurcation aneurysms at different sites (10 anterior communicating artery, 6 middle cerebral artery, 3 basilar artery, 1 vertebral artery/posterior inferior cerebellar artery, 1 internal carotid artery/posterior communicating artery) were treated with 44 closed cell stents (follow-up 2012–2016) using the kissing-Y stenting technique. We analyzed vascular angle geometry between the mother and both affected daughter vessels by digital subtraction angiography, before and after stent deployment, using standard working projections. Results Endovascular treatment of wide necked intracranial aneurysms using the kissing-Y stenting technique significantly decreased the angle between the bifurcation branches from 130.4±9.5° to 91.5±9.1° (p<0.0001). Conclusions Kissing-Y stenting in wide necked bifurcation aneurysms leads to vascular angular remodeling of both affected branches. The resulting straightening of the bifurcation angle may prevent aneurysmal recurrence.
Uridine kinase: Altered subunit size or enzyme expression as a function of cell type, growth stimulation, or mutagenesis

Using antibody prepared against pure undine kinase from Ehrlich ascites cells, we have measured the expression of enzyme protein by the Western blot technique. Variations were observed in the Mr of the enzyme subunit for uridine kinase from different species: 32,000 (mouse Ehrlich ascites cells), 30,000 (normal human lymphocytes), 28,000 (mouse tissues), 27,500 (rat tissues). For different normal tissues from the same species, there was no significant variation in the subunit size. Transformed human and mouse cell lines, selected for a deficiency of uridine kinase activity in the presence of inhibitors activated by this enzyme, expressed two cross‐reacting proteins, one with a normal (30,000) and one with a smaller (21,000) subunit molecular weight than was found in the parental cell line (human lymphoma), or only a smaller protein of Mr 25,000 (mouse lymphoma). Our results show that selection protocols using metabolite inhibitors do not always repress the expression of the enzyme but instead may lead to selection of those cells that have a mutation in the uridine kinase gene, resulting in the expression of an inactive enzyme. The expression of uridine kinase protein changes when cells are stimulated to divide. For both mouse fibroblasts and human lymphocytes, expression of urndine kinase protein as well as activity clearly increased after cells were stimulated to grow. In fibroblasts, increases are seen by 3 hr after stimulation, and plateau after 9 hr at a sevenfold increase. In lymphocytes, no change is seen until 12 hr after stimulation, and a plateau is not reached until 72 hr, with a total increase of ∼50‐fold. There has been considerable interest in the possibility of uridine kinase isozymes. Except for cells that have been mutagenized, the present results show that, as judged by subunit molecular weight, there appears to be only one enzyme form in normal and neoplastic cells or in cells in which uridine kinase activity is induced.
Endothelial dysfunction and high cardiovascular risk profile in severe alcoholics improve only partially following a medium-term alcohol withdrawal.

BACKGROUND Little is known about brachial artery flow-mediated vasodilatation (FMD) in active and medium-term withdrawing heavy alcoholics (HA). METHODS FMD and some parameters of cardiovascular (CV) risk were measured in 29 HA (average alcohol intake 135, range 86 to 215 g per day) at baseline and after a 9 ± 7 months withdrawal and in 35 teetotalers. RESULTS HA showed baseline impaired maximal % FMD (8.5 ± 5.4 SD vs. 14.9 ± 7.4, <0.001 vs. teetotalers), higher systolic (SBP) and diastolic (DBP) blood pressure (+24 mm Hg, <0.001; +15 mm Hg, <0.01), uric acid (5.3 ± 1.1 vs. 4.4 ± 0.8 mg/dl, <0.05), high-sensitivity C-reactive protein (hs-CRP; 2.7 ± 2.0 vs. 1.0 ± 0.9 mg/l, <0.02), endothelin-1 (ET-1, 0.88 ± 0.36 vs. 0.37 ± 0.10 pg/ml,<0.001), asymmetric dimethylarginine (ADMA, 0.50 ± 0.21 vs. 0.41 ± 0.12 μmol/l, p < 0.001), homeostasis model assessment of insulin resistance (HOMA-IR) (2.3 ± 1.1 vs. 1.2 ± 0.4, <0.001), and urinary 8-isoprostane (U8-iso-PGF2α) (237.2 ± 172.4 vs. 168.5 ± 96.6 pg/mg creatinine, <0.05). After withdrawal, SBP fell by 15 mm Hg, DBP by 11 mm Hg (p < 0.001), and hs-CRP by 0.94 mg/l (p < 0.02), all remaining still higher than teetotalers (<0.05, 0.01, 0.05 respectively). ET-1, HOMA-IR, and U8-iso-PGF2α were unchanged (p = NS vs. baseline, <0.05 to 0.001 vs. teetotalers). Maximal % FMD rose (to 10.6 ± 6.2, p < 0.04), but it still remained impaired (<0.04 vs. teetotalers). ADMA increased further to 0.64 ± 0.15 μmol/l (<0.05 vs. baseline, <0.02 vs. teetotalers). CONCLUSIONS HA show marked endothelial dysfunction (ED) and high BP, impaired insulin sensitivity, inflammation, increased oxidative stress, and elevated ET-1 and ADMA, which are unaffected or only partially reversed by a medium-term alcohol withdrawal. ED and related abnormalities persist in detoxified alcoholics, thus contributing to a greater CV morbidity and mortality.
Experimental Study of the Intra-Channel Nonlinearity Influence on Single-Band 100G Coherent Optical OFDM Systems

We report on an experimental investigation of the intra-channel nonlinearity effects in single-band 100G coherent optical orthogonal frequency division multiplexing (CO)-OFDM systems. We first show that instead of using traditional single-polarization training symbols (TS's), which suffer from nonlinearity-induced nonuniform phase shifts, correlated dual-polarization TS's should be used in reduced-guard-interval CO-OFDM systems with short symbol durations. Next, we demonstrate that the intra-channel nonlinearity tolerance in CO-OFDM systems can be improved by employing fewer subcarriers for signal generation.
Contrasting short‐ and long‐timescale effects of vegetation dynamics on water and carbon fluxes in water‐limited ecosystems

While it is generally believed that the magnitude and composition of vegetation cover influence land‐atmosphere water and carbon fluxes, observations indicate that in some cases, fluxes are insensitive to land cover contrasts. This seeming inconsistency may be resolved by contrasting fluxes over short and long timescales. To explore this potential contrast, we developed and tested a model designed to simulate daily to decadal land surface water and carbon fluxes and vegetation dynamics for water‐limited ecosystems. The model reproduces (R2 > 0.76) observed daily fluxes under increasing water limitation and captures representative dynamics of leaf area and fractional cover of dominant grass and wood vegetation. We parameterized the model for southern African savannas and conducted two sets of numerical experiments with either having fixed (static) grass and wood covers or allowing them to adjust dynamically with production. Static simulations reveal that the direct effect of rainfall on soil moisture is more important than the prevailing grass and wood cover states in controlling annual transpiration and production. Dynamic simulations indicate sensitivity of daily fluxes to vegetation cover states during high soil water periods. However, depletion of finite soil water prevents an integrated effect from lasting over interstorm to annual timescales. Correspondingly, while seasonal vegetation dynamics enhance seasonality in fluxes, vegetation dynamics have only minor influence on annual transpiration and production. In fact, annual rainfall explains most (R > 0.85) of the temporal variation in annual water and carbon fluxes. Hence, despite alteration of daily and seasonal distributions of fluxes, for water‐limited ecosystems, vegetation dynamics have little effect on annual transpiration and production.
Fast Volumetric Ultrasound B-Mode and Doppler Imaging with a New High-Channels Density Platform for Advanced 4D Cardiac Imaging/Therapy

A novel ultrasound (US) high-channels platform is a pre-requisite to open new frontiers in diagnostic and/or therapy by experimental implementation of innovative advanced US techniques. To date, a few systems with more than 1000 transducers permit full and simultaneous control in both transmission and receiving of all single elements of arrays. A powerful US platform for implementing 4-D (real-time 3-D) advanced US strategies, offering full research access, is presented in this paper. It includes a 1024-elements array prototype designed for 4-D cardiac dual-mode US imaging/therapy and 4 synchronized Vantage systems. The physical addressing of each element was properly chosen for allowing various array downsampled combinations while minimizing the number of driving systems. Numerical simulations of US imaging were performed, and corresponding experimental data were acquired to compare full and downsampled array strategies, testing 4-D imaging sequences and reconstruction processes. The results indicate the degree of degradation of image quality when using full array or downsampled combinations, and the contrast ratio and the contrast to noise ratio vary from 7.71 dB to 2.02 dB and from 2.99 dB to −7.31 dB, respectively. Moreover, the feasibility of the 4-D US platform implementation was tested on a blood vessel mimicking phantom for preliminary Doppler applications. The acquired data with fast volumetric imaging with up to 2000 fps allowed assessing the validity of common 3-D power Doppler, opening in this way a large field of applications.
The heterodimeric association between the membrane proteins of Semliki Forest virus changes its sensitivity to low pH during virus maturation

The budding and the fusion processes of the enveloped animal virus Semliki Forest virus serve the purpose of transporting its nucleocapsid, containing its genome, from the cytoplasm of an infected cell into that of an uninfected one. We show here that, in the infected cell, the viral membrane (spike) proteins p62 and E1 are organized as heterodimers which are very resistant to dissociation in acidic conditions. In contrast, the mature form of the heterodimer, E2E1, which is found in the virus particle and which is generated by proteolytic processing of p62, is very prone to dissociate upon treatment with mildly acidic buffers. We discuss the possibility that this difference in behavior of the intracellular precursor form and the mature form of the spike protein complex represents an important regulatory mechanism for the processes involving membrane binding around the nucleocapsid during budding and membrane release from the nucleocapsid at the stage of virus fusion.
A novel chitosan CpG nanoparticle regulates cellular and humoral immunity of mice.

OBJECTIVE To develop a safe and novel immunoadjuvant to enhance the immunity and resistance of animals against E. coli infection. METHODS An 88-base immunostimulatory oligodeoxynuleotide containing eleven CpG motifs (CpG ODN) was synthesized and amplified by PCR. The chitosan nanoparticle (CNP) was prepared by ion linking method to entrap the CpG ODN that significantly promotes the proliferation of lymphocytes of pig in vitro. Then the CpG-CNP was inoculated into 21-day old Kunming mice, which were orally challenged with virulent K88/K99 E. Coli 35 days after inoculation. Blood was collected from the tail vein of mice on days 0, 7, 14, 21, 28, 35, 42, and 49 after inoculation to detect the changes and content of immunoglobulins, cytokines and immune cells by ELISA, such as IgG, IgA, IgM, IL-2, IL-4, and IL-6. RESULTS The CpG provoked remarkable proliferation of lymphocytes of pig in vitro in comparison with that of control group (P < 0.05). The inoculation with CpG-CNP significantly raised the content of IgG, IgM, and IgA in the sera of immunized mice (P < 0.05). The levels of IL-2, IL-4, and IL-6 in the mice significantly increased in comparison with those in controls (P < 0.05), so was the number of white blood cells and lymphocytes in immunized mice. The humoral and cellular immunities were significantly enhanced in immunized mice, which resisted the infection of E. coli and survived, while the control mice manifested evident symptoms and lesions of infection. CONCLUSIONS CpG-CNP can significantly promote cellular and humoral immunity and resistance of mice against E. coil infection, and can be utilized as an effective adjuvant to improve the immunoprotection and resistance of porcine against infectious disease.
Transmission line model design formula for microstrip antennas with slots

A technique, based on the transmission line model, for the analysis of a rectangular microstrip antenna, in which more than one slot have been embedded in its non-radiating edges, is presented. Using this method, the number and the dimensions of the slots in a microstrip antenna can be calculated, in order to get a desired lower resonant frequency from a much smaller patch than the initial rectangular one.
E FAR-HD : ENHANCED FUZZY ASSOCIATION RULE MINING USING JARO-WINKLER DISTANCE FOR HIGH DIMENSIONAL DATASETS

Association Rule Mining (ARM) with fuzzy logic theory accelerates the easy process of mining of latent frequent or recurrent patterns constructed on their own frequencies in the form of association rules from any transactional and relational datasets containing objects and items to imply the most recent trends in the given dataset. These mined recurrent patterns or fuzzy association rules employ either for physical data analysis or also influenced to compel further mining tasks like categorization (classification) and collecting (clustering) which helps domain area experts to systematize decision-making. In the conception of data mining, generally fuzzy Association Rule Mining (FARM) technique has been expansively adopted in transactional and relational datasets those datasets containing objects and items who have a fewer to medium amount of attributes/dimensions. Few techniques have also implemented for high dimensional dataset also, but whether those techniques have also works well for low dimensional datasets are yet to be proven out. Hence, in this paper we propose E-FAR-HD algorithm which is an enhanced version of FARHD algorithm that premeditated absolutely for large or high-dimensional datasets. We have intended this EFAR-HD algorithm that increases the accuracy of FAR-HD algorithm on the smaller datasets and remove the chances of misses when FAR-HD has tested on smaller datasets such as contact lens or patient dataset.
A Buck-Boost topology based hybrid converter for standalone nanogrid applications

This paper proposes a novel hybrid converter derived from a conventional Buck Boost DC-DC Converter. The principle of Augmented Sinusoidal Pulse Width Modulation (ASPWM) is used to derive the hybrid topology. It provides both AC and DC voltages using a single DC source as input. As a result of ASPWM switching, the voltage across the Buck-Boost stage inductor will contain a line frequency component, which can be extracted by a frequency selective network. The DC voltage gain for buck-boost converter with ASPWM has been derived and it is shown that buck-boost converter acts as a boost converter for all ranges of DC offset under ASPWM switching. The circuit has been extensively simulated in MATLAB/Simulink for various load conditions. The proposed circuit will have applications in standalone nanogrids where the loads will essentially be AC as well as DC.
Eicosanoid production by mouse peritoneal macrophages during Toxoplasma gondii penetration: role of parasite and host cell phospholipases

The metabolism of endogenous arachidonic acid by mouse resident peritoneal macrophages infected in vitro with Toxoplasma gondii was studied. Prelabeling of macrophages with [5,6,8,9,11,12,14,15-3H]arachidonic acid and challenge with tachyzoites for 15 min resulted in a high mobilization of free labeled arachidonic acid (178%) in the culture medium. The parasites also triggered the synthesis of 6-keto-prostaglandin F1 alpha (47%), prostaglandin E2 (44%), leukotrienes C4 and D4 (33%) and 5-, 12-hydroxyeicosatetraenoic acids (155%). The study indicated that during the intracellular development phase of the parasites, 6-keto-prostaglandin F1 alpha (38%), prostaglandin E2 (31%) leukotrienes C4 and D4 (15%), hydroxyeicosatetraenoic acids (43%), and free arachidonic acid (110%) were secreted into the culture medium. Pretreatment of tachyzoites with phospholipase A2 inhibitors (4-p-bromophenacyl bromide and quinacrine) and no calcium in the culture medium resulted in inhibition of tachyzoite penetration into the macrophages and a decrease of the arachidonic acid metabolism. The triggering of the arachidonic acid cascade by T. gondii was dependent on the active penetration of the parasites into the macrophages, whereas preincubation of the macrophages with phospholipase A2 inhibitors did not affect penetration or free arachidonic acid release, thereby supporting a role for parasite phospholipase in the penetration process and in arachidonic acid mobilization from macrophage membrane phospholipids. Moreover, treatment of macrophages with phospholipase A2 inhibitors decreased the activities of the cyclooxygenase and lipoxygenase pathways, also suggesting an activation of host cell phospholipase A2 by the parasite.
Modal vocal fundamental frequency of young adults.

Recordings of 100 male and 100 female young adults who were judged to have normal speech, voice, and hearing were analyzed for vocal fundamental frequency. A technique of determining modal fundamental frequency by systematic screening of the recorded samples through variable filters was employed. The mean of the vocal fundamental frequencies of males was found to be 116.65 hertz and the mean of the vocal fundamental frequencies of females was 217.00 Hz.
VLSI DESIGN OF LMS ADAPTIVE FIR FILTER FOR HIGH SPEED APPLICATION SARSAN

In this paper, we show an effective design for the implementation of a delayed least mean square adaptive filter and low power reconfigurable finite impulse response filter for achieving lower adaptationdelay and area-delay-power efficient implementation. we use a novel partial product generator and propose a strategy for optimized balanced pipelining across the time-consuming combinational blocks of the structure. From synthesis results, we find that the proposed design offers nearly 17% less area-delay product (ADP) and nearly 14% less energy-delay product (EDP) than the best of the existing systolic structures, on average, for filter lengths N = 8,16, and 32. We propose an efficient fixed-point implementation scheme of the proposed architecture, and derive the expression for steady-state error. We show that the steady-state mean squared error obtained from the analytical result matches with the simulation result. Moreover, we have proposed a bit-level pruning of the proposed architecture, which provides nearly 8% saving in ADP and 18% saving in EDP .