Miracle-dz/UniFAR-FTUs huggingface dataset:
Shifts in the Molecular Epidemiology of Campylobacter jejuni Infections in a Sentinel Region of New Zealand following Implementation of Food Safety Interventions by the Poultry Industry

This study provides a unique insight into the effects of food safety interventions implemented in one sector of the food industry on the transmission routes of a major foodborne agent. Following the implementation of food safety interventions by the poultry industry, shifts in the molecular epidemiology of Campylobacter jejuni infections in a sentinel region of New Zealand were observed. Targeted interventions to reduce disease incidence are effective but require continued surveillance and analysis to indicate where further interventions may be beneficial. ABSTRACT In 2006, New Zealand had the highest notification rate of campylobacteriosis in the world, and poultry was considered the leading source of campylobacteriosis. Implementation of food safety interventions by the poultry industry led to a decrease in the campylobacteriosis notification rate. The aim is to examine the impact of targeted food safety interventions implemented by the New Zealand poultry industry on the source attribution of Campylobacter jejuni infections in a sentinel region. Campylobacter jejuni isolates collected from the Manawatu region of New Zealand between 2005 and 2007 (“before intervention”) and 2008 and 2015 (“after intervention”) from human clinical cases, chicken meat, ruminant feces, environmental water, and wild bird sources were subtyped by multilocus sequence typing. Viable counts of Campylobacter spp. from carcasses were analyzed using a zero-inflated Poisson regression model. In the period before intervention, sequence type 474 (ST-474) was the most common sequence type (ST) recovered from human cases, accounting for 28.2% of the isolates. After intervention, the proportion of human cases positive for ST-474 reduced to 9.3%. Modeling indicated that chicken meat, primarily from one supplier, was the main source of C. jejuni infection in the Manawatu region before intervention. However, after intervention poultry collectively had a similar attribution to ruminants, but more human cases were attributed to ruminants than any single chicken supplier. Viable counts on carcasses were lower in all poultry suppliers after intervention. This study provides evidence of changes in the source attribution of campylobacteriosis following targeted food safety interventions in one sector of the food supply chain. IMPORTANCE This study provides a unique insight into the effects of food safety interventions implemented in one sector of the food industry on the transmission routes of a major foodborne agent. Following the implementation of food safety interventions by the poultry industry, shifts in the molecular epidemiology of Campylobacter jejuni infections in a sentinel region of New Zealand were observed. Targeted interventions to reduce disease incidence are effective but require continued surveillance and analysis to indicate where further interventions may be beneficial.
In search of solutions for evidence generation in rehabilitation: the second Cochrane Rehabilitation Methodology Meeting.

The availability of evidence to support rehabilitation has been a long-standing challenge. We believe that now is the time to start a systematic process of improvement. For this reason, Cochrane rehabilitation is producing an effort to improve the methodology used to generate evidence. Two Cochrane Rehabilitation Methodology Meetings (CRMM) have been performed (Paris, France, July 2018; Kobe, Japan, June 2019) and 2 others programmed (Milan, Italy, February 2020; Orlando, USA, March 2020). All material discussed during CRMMs is published. In this issue we have the papers from the Kobe CRMM about blinding, current reporting standards for population and comparison groups, and the protocol of the RCT Rehabilitation Checklists (RCTRACK) project.The articles published in this issue aim to help the rehabilitation scientific community to move forward, to improve the process of generating evidence in rehabilitation, and to strengthen our field as presented to the general scientific community. To accomplish these goals we need to clearly describe our methods and our approach to future rehabilitation research. Not all fields of study can apply the same methodology in science, but each field must define its research methodology and demonstrate the characteristics that make it a specific field of inquiry.
Occurrence and Molecular Phylogeny of Honey Bee Viruses in Vespids

Since the discovery that honey bee viruses play a role in colony decline, researchers have made major breakthroughs in understanding viral pathology and infection processes in honey bees. Work on virus transmission patterns and virus vectors, such as the mite Varroa destructor, has prompted intense efforts to manage honey bee health. However, little is known about the occurrence of honey bee viruses in bee predators, such as vespids. In this study, we characterized the occurrence of 11 honey bee viruses in five vespid species and one wasp from four provinces in China and two vespid species from four locations in France. The results showed that all the species from China carried certain honey bee viruses, notably Apis mellifera filamentous virus (AmFV), Deformed wing virus (DWV), and Israeli acute paralysis virus (IAPV); furthermore, in some vespid colonies, more than three different viruses were identified. In France, DWV was the most common virus; Sacbrood virus (SBV) and Black queen cell virus (BQCV) were observed in one and two samples, respectively. Phylogenetic analyses of IAPV and BQCV sequences indicated that most of the IAPV sequences belonged to a single group, while the BQCV sequences belonged to several groups. Additionally, our study is the first to detect Lake Sinai virus (LSV) in a hornet from China. Our findings can guide further research into the origin and transmission of honey bee viruses in Vespidae, a taxon of ecological, and potentially epidemiological, relevance.
Improving Phenolic Total Content and Monoterpene in Mentha x piperita by Using Salicylic Acid or Methyl Jasmonate Combined with Rhizobacteria Inoculation

The effects of plant inoculation with plant growth-promoting rhizobacteria (PGPR) and those resulting from the exogenous application of salicylic acid (SA) or methyl jasmonte (MeJA) on total phenolic content (TPC) and monoterpenes in Mentha x piperita plants were investigated. Although the PGPR inoculation response has been studied for many plant species, the combination of PGPR and exogenous phytohormones has not been investigated in aromatic plant species. The exogenous application of SA produced an increase in TPC that, in general, was of a similar level when applied alone as when combined with PGPR. This increase in TPC was correlated with an increase in the activity of the enzyme phenylalanine ammonia lyase (PAL). Also, the application of MeJA at different concentrations in combination with inoculation with PGPR produced an increase in TPC, which was more relevant at 4 mM, with a synergism effect being observed. With respect to the main monoterpene concentrations present in peppermint essential oil (EO), it was observed that SA or MeJA application produced a significant increase similar to that of the combination with rhizobacteria. However, when plants were exposed to 2 mM MeJA and inoculated, an important increase was produced in the concentration on menthol, pulegone, linalool, limonene, and menthone concentrations. Rhizobacteria inoculation, the treatment with SA and MeJA, and the combination of both were found to affect the amount of the main monoterpenes present in the EO of M. piperita. For this reason, the expressions of genes related to the biosynthesis of monoterpene were evaluated, with this expression being positively affected by MeJA application and PGPR inoculation, but was not modified by SA application. Our results demonstrate that MeJA or SA application combined with inoculation with PGPR constitutes an advantageous management practice for improving the production of secondary metabolites from M. piperita.
Risk of Cardiovascular Disease Due to General Anesthesia and Neuraxial Anesthesia in Lower-Limb Fracture Patients: A Retrospective Population-Based Cohort Study

The purpose of this study was to analyze the relationship between elevated cardiovascular disease (CVD) risk and type of anesthesia by using the National Health Insurance Research Database (NHIRD) of Taiwan in a one-year follow-up period. We assessed whether general anesthesia (GA) or neuraxial anesthesia (NA) increased CVD occurrence in lower-limb fracture patients. Approximately 1 million patients were randomly sampled from the NHIRD registry. We identified and enrolled 3437 lower-limb fracture patients who had received anesthesia during operations conducted in the period from 2010 to 2012. Next, patients were divided into two groups, namely GA (n = 1504) and NA (n = 1933), based on the anesthetic technique received during surgery. Our results revealed that those receiving GA did not differ in their risk of CVD relative to those receiving NA, adjusted HR = 1.24 (95% CI: 0.80–1.92). Patients who received GA for more than 2 h also did not differ in their risk of CVD relative to those receiving NA for less than 2 h, adjusted HR = 1.43 (95% CI: 0.81–2.50). Moreover, in the GA group (i.e., patients aged ≥65 years and women), no significant difference for the risk of CVD events was observed. In conclusion, in our study, the difference in the risk of CVD between lower-limb fracture patients receiving NA and GA was not statistically significant. The incidence rate of CVD seemed to be more correlated with patients’ underlying characteristics such as old age, comorbidities, or admission to the intensive care unit. Due to the limited sample size in this study, a database which reviews a whole national population will be required to verify our results in the future.
Genome-wide identification and expression profile of the MADS-box gene family in Erigeron breviscapus

The MADS-box gene family encodes transcription factors with many biological functions that extensively regulate plant growth, development and reproduction. Erigeron breviscapus is a medicinal herb used widely in traditional Chinese medicine, and is believed to improve blood circulation and ameliorate platelet coagulation. In order to gain a detailed understanding of how transcription factor expression may regulate the growth of this potentially important medicinal plant, a genome-wide analysis of the MADS-box gene family of E. breviscapus is needed. In the present study, 44 MADS-box genes were identified in E. breviscapus and categorized into five subgroups (MIKC, Mα, Mβ, Mγ and Mδ) according to their phylogenetic relationships with the Arabidopsis MADS-box genes. Additionally, the functional domain, subcellular location and motif compositions of the E. breviscapus MADS-box gene products were characterized. The expression levels for each of the E. breviscapus MADS-box (EbMADS) genes were analyzed in flower, leaf, stem and root organs, and showed that the majority of EbMADS genes were expressed in flowers. Meanwhile, some MADS genes were found to express high levels in leaf, stem and root, indicating that the MADS-box genes are involved in various aspects of the physiological and developmental processes of the E. breviscapus. The results from gene expression analysis under different pollination treatments revealed that the MADS-box genes were highly expressed after non-pollinated treatment. To the best of our knowledge, this study describes the first genome-wide analysis of the E. breviscapus MADS-box gene family, and the results provide valuable information for understanding of the classification, cloning and putative functions of the MADS-box family.
SiOC functionalization of MoS2 as a means to improve stability as sodium-ion battery anode

The development of feasible, scalable, and environmentally-safe electrode materials that provide stable cycling performance are critical for success of beyond lithium rechargeable batteries and supercapacitors. With respect to the sodium-ion battery (SIB) anodes constituting of transition metal dichalcogenides such as molybdenum disulfide (MoS2), poor cycle stability and fast capacity degradation, due to low electronic conductivity and dissolution of chemical species in the electrolyte, hinders use of these promising layered materials as SIB anodes. Herein we report chemical functionalization in MoS2 nanosheets with polymer-derived silicon oxycarbide or SiOC with the aim to preserve MoS2 from dissolution in the SIB organic electrolyte, without compromising its role in sodiation and desodiation processes. Our results suggest that a MoS2–SiOC composite electrode is effective in bringing improved cycle stability to sodium-ion cycling over neat MoS2 even after 100 cycles.
Soybean RNA interference lines silenced for eIF4E show broad potyvirus resistance

Abstract Soybean mosaic virus (SMV), a potyvirus, is the most prevalent and destructive viral pathogen in soybean‐planting regions of China. Moreover, other potyviruses, including bean common mosaic virus (BCMV) and watermelon mosaic virus (WMV), also threaten soybean farming. The eukaryotic translation initiation factor 4E (eIF4E) plays a critical role in controlling resistance/susceptibility to potyviruses in plants. In the present study, much higher SMV‐induced eIF4E1 expression levels were detected in a susceptible soybean cultivar when compared with a resistant cultivar, suggesting the involvement of eIF4E1 in the response to SMV by the susceptible cultivar. Yeast two‐hybrid and bimolecular fluorescence complementation assays showed that soybean eIF4E1 interacted with SMV VPg in the nucleus and with SMV NIa‐Pro/NIb in the cytoplasm, revealing the involvement of VPg, NIa‐Pro, and NIb in SMV infection and multiplication. Furthermore, transgenic soybeans silenced for eIF4E were produced using an RNA interference approach. Through monitoring for viral symptoms and viral titers, robust and broad‐spectrum resistance was confirmed against five SMV strains (SC3/7/15/18 and SMV‐R), BCMV, and WMV in the transgenic plants. Our findings represent fresh insights for investigating the mechanism underlying eIF4E‐mediated resistance in soybean and also suggest an effective alternative for breeding soybean with broad‐spectrum viral resistance.
Evaluation of Electrolyte Concentration and Pro-Inflammatory and Oxidative Status in Dogs with Advanced Chronic Kidney Disease under Dietary Treatment

An integrated study on the effect of renal diet on mineral metabolism, fibroblast growth factor 23 (FGF-23), total antioxidant capacity, and inflammatory markers has not been performed previously. In this study, we evaluated the effects of renal diet on mineral metabolism, oxidative stress and inflammation in dogs with stage 3 or 4 of chronic kidney disease (CKD). Body condition score (BCS), muscle condition score (MCS), serum biochemical profile, ionized calcium (i-Ca), total calcium (t-Ca), phosphorus (P), urea, creatinine, parathyroid hormone (PTH), FGF-23, interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor alpha (TNF-α) and total antioxidant capacity (TAC) were measured at baseline (T0) and after 6 months of dietary treatment (T6). Serum urea, P, t-Ca, i-Ca, PTH, FGF-23, IL-6, IL-10, TNF-α and TAC measurements did not differ between T0 and T6. Serum creatinine (SCr) was increased at T6 and serum PTH concentrations were positively correlated with serum SCr and urea. i-Ca was negatively correlated with urea and serum phosphorus was positively correlated with FGF-23. Urea and creatinine were positively correlated. The combination of renal diet and support treatment over 6 months in dogs with CKD stage 3 or 4 was effective in controlling uremia, acid–base balance, blood pressure, total antioxidant capacity, and inflammatory cytokine levels and in maintaining BCS and MCS.
Predicted Position Error Triggers Catch-Up Saccades during Sustained Smooth Pursuit

Abstract For humans, visual tracking of moving stimuli often triggers catch-up saccades during smooth pursuit. The switch between these continuous and discrete eye movements is a trade-off between tolerating sustained position error (PE) when no saccade is triggered or a transient loss of vision during the saccade due to saccadic suppression. de Brouwer et al. (2002b) demonstrated that catch-up saccades were less likely to occur when the target re-crosses the fovea within 40–180 ms. To date, there is no mechanistic explanation for how the trigger decision is made by the brain. Recently, we proposed a stochastic decision model for saccade triggering during visual tracking (Coutinho et al., 2018) that relies on a probabilistic estimate of predicted PE (PEpred). Informed by model predictions, we hypothesized that saccade trigger time length and variability will increase when pre-saccadic predicted errors are small or visual uncertainty is high (e.g., for blurred targets). Data collected from human participants performing a double step-ramp task showed that large pre-saccadic PEpred (>10°) produced short saccade trigger times regardless of the level of uncertainty while saccade trigger times preceded by small PEpred (<10°) significantly increased in length and variability, and more so for blurred targets. Our model also predicted increased signal-dependent noise (SDN) as retinal slip (RS) increases; in our data, this resulted in longer saccade trigger times and more smooth trials without saccades. In summary, our data supports our hypothesized predicted error-based decision process for coordinating saccades during smooth pursuit.
Análise do desgaste e da sobrecarga de cuidadores/ familiares de idosos com doença de Alzheimer causado pelos sintomas psicológicos e comportamentais* Analysis of caregiver burden of elderly with Alzheimer disease caused by behavioral psychological symptons of dementia

Alzheimer’s disease (AD) is characterized as a degenerative disease that affects manydomains, including behavior and can cause burden in caregivers/family member of these individuals.The aim of the study was to analyze the caregiver burden in relation to the presence and intensityof Behavioral and Psychological Symptoms in Dementia (BPSD). We used socio demographic profile, Neuropsychiatric Inventory (NPI) and NPI - caregiver distress (NPI-D) for our evaluation.We interviewed 30 caregivers of elderly with Alzheimer disease according to NINCDS-ADRDAcriteria, divided in mild level (n=15) and moderate level (n=15) according to DSM-III-R criteria.The most prevalent symptoms were, in the mild level, anxiety and depression, and in the moderatelevel they were anxiety and agitation. The analyses conducted between caregivers’ distress andBPSD showed us that the symptoms with the greatest correlation in the mild level were agitationfollowed by disinhibition. In relation to caregiver burden there was a significant correlation withdelirium in mild level and apathy in moderate level. In conclusion, it was observed that the BPSDthat affect the burden caregivers differ according to the level of the disease and the knowledge ofthese impacts allows us to reflect and propose actions to implement public policies and interventionsin the care of the elderly and their caregivers/family members
Autophagy Promotes Porcine Parvovirus Replication and Induces Non-Apoptotic Cell Death in Porcine Placental Trophoblasts

Autophagy plays important roles in the infection and pathogenesis of many viruses, yet the regulatory roles of autophagy in the process of porcine parvovirus (PPV) infection remain unclear. Herein, we show that PPV infection induces autophagy in porcine placental trophoblasts (PTCs). Induction of autophagy by rapamycin (RAPA) inhibited the occurrence of apoptotic cell death, yet promoted viral replication in PPV-infected cells; inhibition of autophagy by 3-MA or ATG5 knockdown increased cellular apoptosis and reduced PPV replication. Interestingly, we found that in the presence of caspase-inhibitor zVAD-fmk, PPV induces non-apoptotic cell death that was characterized by lysosomal damage and associated with autophagy. Induction of complete autophagy flux by RAPA markedly promoted PPV replication compared with incomplete autophagy induced by RAPA plus bafilomycin (RAPA/BAF) in the early phase of PPV infection (24 h.p.i.). Meanwhile, induction of complete autophagy with RAPA increased lysosomal damage and non-apoptotic cell death in the later phase of PPV infection. Therefore, our data suggest that autophagy can enhance PPV replication and promote the occurrence of lysosomal-damage-associated non-apoptotic cell death in PPV-infected porcine placental trophoblasts.
The relationship between weight indices and injuries and mortalities caused by the motor vehicle accidents: a systematic review and meta-analysis

Abstract: Background: The relationship between weight indices and injuries and mortality in motor vehicle accidents is unknown. Systematic review studies addressing the collection and analysis of the relationship in investigations are very limited. The purpose of this systematic review is to deter-mine the relationship between BMI, obesity and overweight with mortality and injuries and their severity and vulnerable organs after the motor vehicle accident. Methods: The databases (MEDLINE/PUBMED, EMBASE, Web of Science, etc) were searched for relevant abstracts using certain keywords. Of all the articles, similar ones were removed considering different filters. The collected data were entered into the STATA SE v 13.1. The heterogeneity of the data was analyzed using i2 statistics. In addition, the estimates of the study were done based on the age group (children and adults) and the impact of obesity on different regions of the body. Results: A direct relationship was observed between the overall BMI and the degrees of injuries (CI=0.503-1.139), and mortality due to motor vehicle accident (CI=1.267-1.471). A positive relationship was found between obesity and AIS+2 (CI=0.653-1.426), and AIS+3 (CI=1.184-1.741), and ISS (CI=1.086-1.589). Also, a negative relationship between overweight and inju-ries rates, and a direct relationship between overweight and mortality (CI=0.979-1.167), and injuries with index of AIS+2 (CI=1.178-0.768) and AIS+3 (CI=0.48-2.186) were found. Conclusions: The prediction of injury, mortality and severity of injuries in the motor vehicle accident by the variable of obesity and overweight determines the need to design prevention programs for this vulnerable group at all levels.