Miracle-dz/UniFAR-FTUs huggingface dataset:
SHORT COMMUNICATION Transient forebrain ischemia induces persistent hyperactivity of large conductance Ca 2+ -activated potassium channels via oxidation modulation in rat hippocampal CA1 pyramidal neurons

The present study examined temporal changes in activity of large conductance, Ca 2+ -activated potassium (BKCa) channels in postischemic CA1 pyramidal neurons at 2, 6, 24 and 48 h after reperfusion. These changes in activity and possible cellular mechanisms were examined using the inside‐out configuration of patch clamp. The unitary conductance of postischemic BKCa channels increased transiently to 119% of the control at 2 h after reperfusion, and recovered to the control level thereafter. A persistent increase in [Ca 2+ ]i sensitivity of BKCa channels was observed in postischemic CA1 neurons with the maximal sensitivity to [Ca 2+ ]i at 6 h after reperfusion while channel voltage- dependence showed no obvious changes. Kinetic analyses showed that the postischemic enhancement of BKCa channel activity was due to longer open times and shorter closed times as there was no significant changes in opening frequency after ischemia. Glutathione disulphide markedly increased BKCa channel activity in normal CA1 neurons, while reducing glutathione caused a decrease in BKCa channel activity by reducing the sensitivity of this channel to [Ca 2+ ]i in postischemic CA1 neurons. Similar modulatory effects on postischemic BKCa channels were also observed with another redox couple, DTNB and DTT, suggesting an oxidation modulation of BKCa channel function after ischemia. The present results indicate that a persistent enhancement in activity of BKCa channels, probably via oxidation of channels, in postischemic CA1 pyramidal neurons may account for the decrease in neuronal excitability and increase in fAHP after ischemia. The ischemia-induced augmentation in BKCa channel activity may be also associated with the postischemic neuronal injury.
Resonable Selection of Antibiotics during Perioperative Period in Cesarean Section

Objective:To investigate the optimal antibiotics to prevent postoperative infection during perioperative period in cesarean section.Methods:A total of 673 patients enrolled in the study were divided into 3 groups randomly,Group A(Ampicillin,n=232),Group B(Cefazolin,n=223)and Group C(Ceftriaxone,n=218). The antibiotics were injected by intravenous drop infusion 30 minutes before operation,immediately after operation and 6 hours after operation in group A and B,but in group C without the time point immediately after operation.The effects on prevention of infection by the antibiotics were evaluated.Results:There was no significant difference among these 3 groups in the incidence of fever and hemogram abnormality(P0.05). The rate of abdominal incision postoperative infections was significantly lower in Group A compared with Group B and Group C(P0.05),while there was no significant difference between Group B and Group C(P0.05). There was no intrauterine infection,abdominopelvic cavity or urinary system infection among these three groups.Conclusions:Using Ampicillin to prevent postoperative infection during perioperative period is a widespread,promotional schedule which has the advantages of good effects,safety and cheap.
SYNTHESIS AND CHARACTERIZATION OF BENZOTRIAZOLIUM-BASED IONIC LIQUIDS FOR TECHNETIUM-99M SEPARATION FROM MOLYBDENUM BY IL-MEDIATED EXTRACTION PROCESS

Various separation techniques for separation of technetium-99m ( 99m Tc) from molybdenum-99 ( 99 Mo) are being developed to overcome the drawbacks of using 99 Mo from neutron activation technique. Ionic liquids (ILs) were used in many extraction processes in metal separation due to their high selectivity. In this research, two benzotriazolium-based ionic liquids were used as co-extractant in 99m Tc/ Mo separation process via liquid-liquid extraction. 1-octyl-3-methyl-benzotriazolium iodide ([MeOcBtu]I) and 1-octyl-3-methyl-benzotriazolium bis (trifluoromethanesulfonyl) imide ([MeOcBtu]TF 2 N) were successfully synthesized and analyzed by FTIR, 1 H-NMR and 13 C-NMR. Extraction processes were conducted in various organic solvents, pH, and extraction time without/ with ionic liquids addition. The data of ratio between 99m Tc distribution coefficient compared to Mo distribution coefficient showed that the addition of ionic liquids exhibited significant improvement of separation factor. The separation factor of extraction using conventional water immiscible solvent ranged between 0 – 8 and increased to 30 – 600 as ILs were added. The optimum conditions which achieved highest separation factor were pH 14 using [MeOcBtu]I-chloroform. Benzotriazolium-based ionic liquid potential to be developed as extractant in the separation of 99m Tc from 99 Mo. Keywords : Ionic liquids, benzotriazolium, technetium-99m, molybdenum, liquid-liquid extraction.
Unsupervised Pattern Recognition Chemometrics for Distinguishing Different Egyptian Olive Varieties Using a New Integrated Densitometric Reversed-Phase High-Performance Thin-Layer Chromatography—Image Analysis Technique

The merits of chemometrics in categorizing different Egyptian olive chemovarieties based on their compositional integrity were implemented in this study. Fingerprints of 9 different olive leaves varieties cultivated in Egypt were established using reversed-phase high-performance thin-layer chromatography (RP-HPTLC) prior to and after post-chromatographic derivatization with natural product–polyethylene glycol (NP/PEG) reagent and image analysis using ImageJ® software in order to build 2 separate data matrices. The chromatographic fingerprints were separately subjected to unsupervised pattern recognition multivariate analysis to build 2 separate models using principal component analysis (PCA) and hierarchical clustering analysis (HCA) algorithms to explore the distribution pattern of different chemovarieties. The second model which involved olive samples’ fingerprints after post-chromatographic derivatization exhibited greater ability to reveal a broader spectrum of phytoconstituents with enhanced sensitivity. Densitometric RP-HPTLC quantification of oleuropein marker was compared to image analysis approach using Sorbfil TLC Videodensitometer® by newly developed and validated methods. Densitometry exhibited better performance characteristics than image analysis method and therefore was executed for determination of oleuropein concentration in the 9 Egyptian olive varieties. Oleuropein marker solely was found to be inadequate for standardization of olive leaves varieties. This study demonstrated a comprehensive approach for the rapid classification of different Egyptian olive varieties, which is crucial to warranting their chemical-consistency and, thereafter, effective consistency.
Synergistic Effect of PPFD and Mycorrhization for Efficient in vitro Propagation of Dendrobium chrysanthum Wall. ex Lindl.

Orchids of the family Orchidaceae are one of the largest angiosperm families of the plant kingdom. Over the past 40 years orchids have been subjected to high commercial demand for their beautiful and vivid colored flowers, delicate scents and medicinal properties. However, increased biotic influences e.g., deforestation, socio-economic development and excessive exploitation for commercial purposes have led to a considerable depletion International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 10 (2019) Journal homepage: http://www.ijcmas. com
TARGETING Kv2.1/SYNTAXIN INTERACTION FOR NEUROPROTECTION

The regulation of cytosolic potassium is a convergent factor in cell death programs of many mammalian cell types including central nervous system neurons. Physiological levels of potassium concentrations can suppress the activation of critical caspases and nucleases necessary for cell death. Under physiological conditions, several ion channels and exchangers sustain intracellular potassium levels. Conversely, tightly regulated molecular pathways facilitate the depletion of intracellular potassium after injury to very low concentrations, enabling cell death mechanisms to proceed. Several research groups have shown that preventing the loss of intracellular potassium after injury through various approaches can increase the survivability of several cell types. In neurons, the main regulator of intracellular potassium after injury is the delayed rectifier potassium channel Kv2.1. Strategies aimed to ameliorate Kv2.1-dependent neuronal cell death have been investigated over the past several years. We have come to understand that lethal oxidative damage set forth unique, zinc-dependent phosphorylation of Kv2.1, leading to enhanced membrane channel insertion and elevated potassium efflux currents. Critical to this pathway is the protein-protein interaction between Kv2.1 and the cell surface soluble NSF attachment protein receptor (SNARE) syntaxin 1A (syntaxin). Interrupting this interaction has been shown to improve neuronal survival in vitro. In this dissertation, I report several studies that further clarify the molecular interactions between Kv2.1 and syntaxin, and provide the first in vivo evidence that disrupting the Kv2.1-syntaxin binding is a viable neuroprotective strategy. We explored several approaches using both peptide-based and synthetic small molecules as the protective agent. To cap off these findings, I provide preliminary data that leverages the hepatitis virus protein NS5A to suppress Kv2.1-dependent cell death in ischemic stroke. We intend for the work presented here to serve as the basis to further unravel the role of Kv2.1 in other neurodegenerative conditions and eventually make the translational leap to improve clinical treatments.
Experimental Data Treatment of the Pipeline Steel Polarization Curve under AC Interference

The treatment of polarization curves of pipeline steels subject to AC (Alternating Current) was studied. The Tafel exploration, the non-linear curve fitting method, and the SYMADEC (synthesis, matching, and deconvolution of polarization curves) algorithm were used to evaluate the Tafel slopes and the corrosion current density from a set of experimental polarization curves. The results of the Tafel exploration show a large variation when different linear portions were selected for the calculation. The non-linear curve fitting method is sensitive to noise and easy to fail when used to fit planarization data presenting AC signal. SYMADEC is a robust method to fit the experimental polarization curves, which do not present a Tafel region. Moreover, the results of SYMADEC can be deconstructed into the anodic and cathodic components providing more kinetics information about the electrode process. Therefore, in the study of the AC corrosion behavior by polarization curves exhibiting no Tafel region, the experimental data should be carefully treated to obtain accurate values of the kinetics parameters. The use of SYMADEC is recommended in this case.
Uniformly 13C Labeled Lignin Internal Standards for Quantitative Pyrolysis−GC−MS Analysis of Grass and Wood

With the ever-advancing lignocellulose valorization strategies, lignin analyses need to advance as well. However, lignin quantification still heavily relies on unspecific, time- and sample-consuming gravimetric, and spectrophotometric analyses. Here, we demonstrate that lignin isolates from uniformly 13C-labeled wheat straw, willow, and douglas fir serve as “ideal” internal standards for pyrolysis gas chromatography high-resolution mass spectrometry (py–GC–HR-MS) analyses of plant biomass, allowing the accurate and precise quantification and structural characterization of lignin in grasses, hardwoods, and softwoods. The 13C lignin internal standards were comprehensively structurally characterized by HSQC NMR and py–GC–HR-MS analyses, and their application for lignin quantification was validated in biomass model systems and in actual plant biomass. For all botanical origins and species, the lignin content was determined within 5% relative deviation of the Klason benchmark. These results establish the capab.
Open-label, multicenter, phase II trial of ABI-007 in previously treated and previously untreated patients with metastatic malignant melanoma

7558 Background: ABI-007 (Abraxane TM), is an albumin-bound, nanoparticle form of paclitaxel with an improved therapeutic index that may be related to biological interactions with endogenous albumin receptors (eg, gp60) and binding proteins (eg, SPARC). The albumin-binding protein SPARC (osteonectin) was overexpressed in primary and metastatic malignant melanomas but not in normal melanocytes (Ledda et al, J Invest Derm 108:210, 1997) and was a predictor of clinical outcome in thin melanomas (Massi et al, Human Path 30:339, 1999). Methods: We conducted a Phase II study of ABX administered weekly for 3 weeks q4w without premedication in chemotherapy-naive (CN) (150 mg/m2) and previously treated (PT) (100 mg/m2) patients (pts) with measurable metastatic malignant melanoma (MMM). Results: 13 CN and 36 PT pts have been enrolled; 11 CN and 13 PT pts are currently evaluable for toxicity and response. Median age was 59 (range, 32–79); median number of metastatic sites was 2 (range, 1–8); median ECOG PS was 0 (ra.
V. Nerve Fibers of Spinal and Vagus Origin Associated with the Cephalic Sympathetic Nerves

The sympathetic nerves in the cephalic region, according to the current teaching, are derived from the superior cervical sympathetic ganglia and consist wholly of postganglionic fibers. These fibers, having their origin in the superior cervical sympathetic ganglia, extend into the cephalic region mainly through the nerves associated with the internal and external carotid arteries. Clinical data affording evidence of the existence in the head and face of afferent nerve fibers which enter the central nervous system through spinal nerves have long been available. For example, Sluder (1920) described a syndrome having its origin in the orbit or the mucous membranes of the nose and paranasal sinuses which not infrequently includes pain referred to the shoulder, arm, forearm and hand. Herpetic eruptions on the chest accompanying lesions of the nasal mucosa and the mastoid also have been reported. Terracol (1932) cited a case in which operation for the relief of nasal obstruction was followed by eruption on the chest and another in which dressings of the mastoid wound caused violent pains in the arm and eruptions on the chest. Failure of section of the sensory root of the trigeminal nerve to relieve the pain of facial neuralgia in certain atypical cases also has been reported by not a few surgeons. In certain cases the pain of facial neuralgia has persisted following section of the nervus intermedius, in addition to section of the sensory root of the trigeminal nerve. These phenomena have called attention to the possible role in atypical facial neuralgia of fibers associated
Long Non-Coding RNAs Are Commonly Deregulated in Hodgkin Lymphoma

Hodgkin’s lymphoma (HL) is a B-cell neoplasm characterized by a minority of neoplastic cells within an extensive inflammatory background. Long non-coding RNAs (lncRNAs) have diverse roles in transcriptional, posttranscriptional and epigenetic mechanisms in normal cell physiology. Deregulation of lncRNA expression has been implicated in a number of cancers. Up to date it is unclear to what extent lncRNAs are involved in the pathogenesis of Hodgkin lymphoma (HL). We designed a custom made microarray based on a published lncRNA catalog of 10,509 lncRNA loci (Cabili et al., 2011). The array also contains probes for all protein coding genes allowing simultaneous detection of the lncRNA and mRNA expression pattern. We analyzed 6 HL cell lines and three samples of purified tonsillar naive, germinal center (GC) and memory B cells. To get more insight into the potential function of individual differentially expressed lncRNAs we determined lncRNA enrichment in cytoplasmic and nuclear fractions of two HL cell lines. Furthermore, we studied to what extent lncRNAs are enriched in the Argonaute 2 (Ago2) containing complexes as an indication for interaction with miRNAs. In a comparison between the three different normal B-cell subsets, we observed 565 differentially expressed lncRNA probes. Overall, naive and memory B cell subsets showed a highly similar expression pattern while GC B cells showed a distinct lncRNA expression pattern. A significant differential expression between HL and normal GC B-cells was observed for 717 lncRNA probes. Interestingly, 109 of those overlap with the 565 lncRNAs differentially expressed between the normal B cell subsets. Differential expression for 4 in HL up and 2 downregulated lncRNAs was confirmed using qRT-PCR. To get a first indication about their potential function, we evaluated the subcellular location of the lncRNAs by comparing the abundance in nuclear and cytoplasmic fractions relative to the total fraction. Overall, 26% of the expressed lncRNAs were consistently enriched in the nuclear fraction and 10% were predominantly enriched in the cytoplasmic fraction. Of the 717 differentially expressed lncRNAs 82 were consistently enriched in the nucleus and 16 in the cytoplasm. Our Ago2 pull-down experiments revealed that 9% of the mRNAs and 3% of the expressed lncRNAs are consistently enriched in Ago2 complexes. In total, we observed evidence for miRNA binding for 17 of the in HL differentially expressed lncRNAs. In conclusion, we identified a specific lncRNA expression pattern in GC B cells and observed for more than 700 lncRNAs a differentially expression pattern between HL and normal GC B-cells. For 98 lncRNAs we found a specific subcellular enrichment and for 17 we observed enrichment in the Ago2 fraction, indicative of miRNA-lncRNA interactions. Follow-up studies will determine to what extent these lncRNAs contribute to the pathogenesis of Hodgkin lymphoma. Disclosures: No relevant conflicts of interest to declare.
The Association between Epstein-Barr virus (EBV) Past Infection with the Risk of Oral Squamous Cell Carcinoma (OSCC)

The association of Epstein-Barr virus (EBV) with oral cancer has been widely reported in the past. However, previous studies mainly focused on the current infection of EBV without acknowledging the possibility of past infection in patients which may lead to oral cancer development. The present study aims to investigate the correlation between past EBV infections with Oral Squamous Cell Carcinoma (OSCC). Both Immunoglobulin M (IgM) and Immunoglobulin G (IgG) antibodies against EBV were screened to detect the presence of EBV in sera of OSCC patients using Enzyme-Linked Immunosorbent Assay (ELISA). The use of IgM antibody against EBV confirms current infection in patients, whereas IgG antibody would predict past infection throughout patients’ lifetime. Through the present study, we would be able to confirm whether patients with past EBV infection have a significant risk in developing oral cancer. ELISA tests were carried out to detect the presence of EBV IgG and IgM in 206 OSCC and control serum samples. Statistical analysis was performed using SPSS 12.0. 1. Our results had shown that 96.6% (n = 199) of OSCC samples and 97.2% (n = 130) control were positive with EBV VCA IgG, however, none of the OSCC and control samples was positive for EBV VCA IgM. The presence of EBV VCA IgG in both OSCC and control suggest that past EBV infection does not play a significant role as a risk indicator for OSCC. Therefore, the association between EBV and OSCC was not well demonstrated in this study.
The success of single débridement and primary closure of acute postoperative infections after open reduction and internal fixation

Background:The development of surgical site infection in the presence of hardware after open reduction and internal fixation is an ongoing dilemma. There are no evidence-based guidelines to guide the number of surgical débridements and irrigations before definitive wound closure. The purpose of the present study was to assess the prevalence of infection resolution using a single debridement and primary wound closure in the acute postoperative infection and to identify risk factors for failure of single débridements and primary wound closure. Methods:We retrospectively reviewed 61 postoperative infections (60 patients) after open reduction and internal fixation of fractures that were treated with a single débridement and primary closure. For the analysis, the cases were categorized as the success group (n=29) and the failure group (n=32). Data were collected from a review of the patients’ medical record. Variables were compared between the two groups using univariate and multivariate logistic regression analysis. Results:Twenty-nine infections (48%) in 28 patients achieved successful outcome after single débridement and primary closure without hardware removal. Risk factors for treatment failure, identified by multivariate analysis, were Orthopaedic Trauma Association (OTA) classification B and C fractures (odds ratio=2.489; 95% confidence interval=1.085, 5.713; P=0.031) and C-reactive protein at admission (odds ratio=1.019; 95% confidence interval=1.004, 1.034; P=0.014). Conclusions:Acute postoperative infection after open reduction and internal fixation treated by single débridement with primary closure and hardware retention is successful in only half of the infections. This result may be improved by patient selection based on certain risk factors.
Analysis of flavour compounds and prediction of sensory properties in sea buckthorn (
 Hippophaë rhamnoides
 L.) berries

The aim of this study was to investigate sugars, organic acids, flavonol glycosides (FGs), proanthocyanidins and volatiles as flavour compounds in sea buckthorn (SB) berries of five cultivars and to predict the sensory properties of berries. The profiles of flavour compounds in SB berries varied significantly among the cultivars. Total proanthocyanidins and FGs were highest in ‘Pertsik’ and ‘Raisa’, respectively. Total volatiles were highest in ‘Vorobyevskaya’ and lowest in ‘Raisa’. A previously established PLS model was used to predict the sensory properties of SB berries based on the non‐volatile flavour compounds. The mouth‐drying astringency can be predicted the most reliably, which has the highest regression coefficients with quinic acid, isorhamnetin‐3‐O‐sophoroside‐7‐O‐rhamnoside and total proanthocyanidins. Bitterness cannot be predicted using the model. ‘Pertsik’ berries were predicted to be more mouth‐drying astringency and sour than those of ‘Raisa’. The research supports the cultivar selection in cultivation and industry of SB berries.