Miracle-dz/UniFAR-FTUs huggingface dataset:
Behavioural and pharmaceutical interventions for the prevention of skin cancers in solid organ transplant recipients: a systematic review of randomised controlled trials

Objectives Solid organ transplant recipients are at increased risk of skin cancer, affecting more than 50% of recipients. We aimed to determine the effectiveness of interventions for behavioural change for sun protection or skin cancer prevention in solid organ transplant recipients. Design Systematic review. Data sources We searched MEDLINE, Embase, the Cochrane Central Register of Controlled Trials (CENTRAL) and CINAHL from inception to November 2019. Eligibility criteria We included randomised controlled trials that evaluated the effect of behavioural or pharmaceutical interventions on behavioural change or skin cancer prevention in solid organ transplant recipients. Data extraction and synthesis Risks of bias and evidence certainty were assessed using Cochrane and the Grading of Recommendations Assessment Development and Evaluation framework. Results Twenty trials (n=2295 participants) were included. It is uncertain whether behavioural interventions improve sun protection behaviour (n=3, n=414, standardised mean difference (SMD) 0.89, 95% CI −0.84 to 2.62, I2=98%) and knowledge (n=4, n=489, SMD 0.50, 95% CI 0.12 to 0.87, I2= 76%) as the quality of evidence is very low. We are uncertain of the effects of mammalian target of rapamaycin inhibitors on the incidence of non-melanocytic skin cancer (n=5, n=1080, relative risk 0.46, 95% CI 0.28 to 0.75, I2 =72%) as the quality of evidence is very low. Conclusions Behavioural and pharmaceutical preventive interventions may improve sun protective behaviour and knowledge, and reduce the incidence of non-melanocytic skin cancer, but the overall quality of the evidence is very low and insufficient to guide decision-making and clinical practice. PROSPERO registration number CRD42017063962.
Feasibility and utility of transbronchial cryobiopsy in precision medicine for lung cancer: Prospective single‐arm study

Cryoprobe is a novel transbronchial biopsy (TBB) tool that yields larger tissue samples than forceps. Pathological diagnosis and biomarker analysis, such as genetic alterations and programmed death‐ligand 1 (PD‐L1) expression, are paramount for precision medicine against lung cancer. We evaluated the safety and usefulness of cryoprobe TBB for lung cancer diagnosis and biomarker analysis. In this single‐center, prospective single‐arm study, patients suspected of having or diagnosed with primary lung cancer underwent cryoprobe TBB using flexible bronchoscopy after conventional forceps TBB from the same lesion. Cryoprobe TBB was performed in 121 patients. The incidence rate of severe bleeding and serious adverse events (4% [90% confidence interval: 2%‐9%]) was significantly lower than the expected rate (20% with 30% threshold, P < 0.01). Combining both central and peripheral lesions, the diagnostic yield rate of cryoprobe samples was 76% and that of forceps samples was 84%. Compared with forceps TBB samples, cryoprobe TBB samples were larger (cryoprobe 15 mm2 vs forceps 2 mm2) and resulted in a larger proportion of definite histomorphological diagnosis (cryoprobe 86% vs forceps 74%, P < 0.01), larger amounts of DNA extracted from samples (median: cryoprobe, 1.60 µg vs forceps, 0.58 µg, P = 0.02) and RNA (median: cryoprobe, 0.62 µg vs forceps, 0.17 µg, P < 0.01) extracted from samples, and tended to yield greater rates of PD‐L1 expression >1% (51% vs 42%). In conclusion, cryoprobe is a safe and useful tool for obtaining lung cancer tissue samples of adequate size and quality, which allow morphological diagnosis and biomarker analysis for precision medicine against lung cancer.
Prospective evaluation of vascular changes in acute respiratory infections in children with cystic fibrosis1 The preliminary results of this study were presented as an oral presentation at the 3nd Middle East Cystic Fibrosis Conference(21-23 March 2019, Istanbul, Turkey).

Background/aim Acute exacerbations and chronic inflammation are risk factors for cardiovascular disease (CVD) in cystic fibrosis (CF) patients. The aim of this study was to investigate the effects of acute exacerbation therapy on arterial stiffness in children with CF. Materials and methods Augmentation index (Aix) and pulse wave velocity (PWV) were measured before and after treatment and 1 month after the end of treatment in patients with acute exacerbation. The relationship between hemodynamic measurements and c-reactive protein (CRP) and pulmonary function tests (PFTs) was investigated. Results Measurements before and after treatment were evaluated in 27 patients and were repeated in 21 patients who were clinically stable 1 month following acute exacerbation. There was a significant decrease in CRP and an increase in spirometry parameters after treatment. While no significant difference was found between PWV (P = 0.33), a significant difference for Aix before (41.95 ± 12.96%) and after (30.95 ± 11.47%) treatment and before treatment and stable clinical condition (34.19 ± 14.36%) was obtained (P =0.00, and P =0.01, respectively). No significant difference in heart rate and other hemodynamic measurements was found. Pretreatment Aix is associated with poor clinical condition (PFTs, BMI, and clinical score) and systemic inflammation (CRP) (P <0.05). Conclusion The decrease of arterial stiffness (Aix) with acute exacerbation treatment in children with CF has been demonstrated. This result shows that systemic inflammation in CF may cause an increase in arterial stiffness and recurrent exacerbations may increase the risk of CVD.
Electrophysiological and behavioural responses of Melolontha melolontha to saturated and unsaturated aliphatic alcohols

Male Melolontha cockchafers are known to use green leaf volatiles induced by female feeding on host plants for their mate location. Earlier studies of the response of the European cockchafer, Melolontha melolontha L. (Coleoptera: Scarabaeidae), to different green leaf aldehydes, alcohols, and acetates revealed that only green leaf alcohols were attractive to males in the field. Females were not attracted at all by these volatiles. Here, we present a study that aimed to elucidate the structure–activity relationships of aliphatic alcohols. Both behavioural and physiological responses were studied in male and female M. melolontha by field tests and electroantennography. The compounds tested were saturated aliphatic alcohols with chain lengths between five and eight carbon atoms. Furthermore, the cockchafer's response to six‐carbon alcohols with (E)‐2‐, (E)‐3‐, (Z)‐2‐, (Z)‐3‐, and (Z)‐4‐configurated double bonds was tested. All compounds elicited dose‐dependent responses on the antennae of both sexes. In general, males showed a stronger normalized EAG response to the stimuli than females. However, only the naturally occurring six‐carbon alcohols, i.e., 1‐hexanol (E)‐2‐, (Z)‐3, and (E)‐3‐hexen‐1‐ol were attractive to M. melolontha males in the field. Females were not attracted to any of the tested compounds, confirming previous results on the olfactory orientation of Melolontha cockchafers.
Distinguishing between hidden testes and anorchia: The role of endocrine evaluation in infancy and childhood.

OBJECTIVE Non-palpable testes remain a diagnostic challenge, often involving exploratory laparoscopy. We evaluated the diagnostic value of a wide range of reproductive hormones in order to distinguish between bilateral cryptorchidism and bilateral anorchia. DESIGN In this retrospective study, we identified and included 36 boys with non-palpable testes (20 with cryptorchidism, 3 with congenital hypogonadotropic hypogonadism (CHH), and 13 with anorchia) at first examination during childhood. METHODS Information on karyotype, phenotype, surgical results from laparoscopy, and biochemistry was retrieved from patient files. We compared serum concentrations of AMH, inhibin B, FSH, LH, testosterone (T), estradiol, and hCG stimulation testing in cryptorchid and anorchid boys to serum concentrations in a large, age-matched control group. Receiver operating characteristic curves were used to determine cut-off values of each reproductive hormone as a predictor of the presence of functional testicular tissue. RESULTS Concentrations of AMH in 0-1 year olds: ≥155 pmol/L and >1-15 year olds: ≥19 pmol/L, inhibin B (≥22 pg/mL and ≥4 pg/mL), FSH (≤28.9 IU/L and ≤20.3 IU/L) and hCG-induced T (>1-15 year olds: ≥2 nmol/L) were significantly sensitive and specific markers in predicting the presence of functional testicular tissue in boys with non-palpable testes. In infancy, anorchid infants had significantly elevated gonadotropin levels while CHH had low levels. CONCLUSION Our findings suggest that laparoscopy may not be necessary in all boys with non-palpable testes if reproductive hormones unequivocally confirm the presence of functional testicular tissue while proving the absence may still be a diagnostic challenge.
Corin Overexpression Reduces Myocardial Infarct Size and Modulates Cardiomyocyte Apoptotic Cell Death

Altered expression of corin, a cardiac transmembrane serine protease, has been linked to dilated and ischemic cardiomyopathy. However, the potential role of corin in myocardial infarction (MI) is lacking. This study examined the outcomes of MI in wild-type vs. cardiac-specific overexpressed corin transgenic (Corin-Tg) mice during pre-MI, early phase (3, 24, 72 h), and late phase (1, 4 weeks) post-MI. Corin overexpression significantly reduced cardiac cell apoptosis (p < 0.001), infarct size (p < 0.001), and inhibited cleavage of procaspases 3, 9, and 8 (p < 0.05 to p < 0.01), as well as altered the expression of Bcl2 family proteins, Bcl-xl, Bcl2 and Bak (p < 0.05 to p < 0.001) at 24 h post-MI. Overexpressed cardiac corin also significantly modulated heart function (ejection fraction, p < 0.0001), lung congestion (lung weight to body weight ratio, p < 0.0001), and systemic extracellular water (edema, p < 0.05) during late phase post-MI. Overall, cardiac corin overexpression significantly reduced apoptosis, infarct size, and modulated cardiac expression of key members of the apoptotic pathway in early phase post-MI; and led to significant improvement in heart function and reduced congestion in late phase post-MI. These findings suggest that corin may be a useful target to protect the heart from ischemic injury and subsequent post-infarction remodeling.
Antigen-Antibody Interaction

The purpose of this experiment is to illustrate how antigen-antibody interactions can be applied to the study of evolution. Antigens are substances that stimulate an immune response from the body. There are two general categories of antigens: pathogens and allergens (Karp 1996). Pathogens include viruses, bacteria, fungi, protozoan parasites and toxins. Allergens include pollen, spores, and food additives. Antibodies are globular proteins produced by B lymphocytes that bind to an antigen and target the antigen for destruction. Antibodies are located in interstitial fluid and plasma and are mostly effective against pathogens that have not invaded host cells. The Ouchterlony test is a double diffusion method on an agar plate that clearly shows any reaction between antigens and antibody (Ouchterlony & Nilsson 1973). Antigens and antibody are inoculated onto an agar plate into separate wells where they diffuse through the medium. As long as the pore sizes within the agar gel substance are large enough, an assumption is made that diffusion of randomly moving particles can freely occur. The agar must be an inert material that does not react with the testing materials. If the antibody recognizes and binds to an antigen on the plate, a white precipitate will form. The precipitate itself is too large to continue to diffuse-once a precipitate is formed, it is stationary. The antigens for this experiment are albumins from various ungulates: horses, pigs, cows and sheep. Albumins are small globular proteins isolated from blood. Albumin is produced by the liver and participates in homeostasis by influencing the osmotic balance of plasma. Antiserum is a liquid containing antibodies that have been exposed to a specific antigen. For example, antihorse serum includes antibodies that will react against antigens isolated from a horse. Antisera were purchased from Sigma, a chemical supply company (PO Box 14508, St. Louis, MO 63178, USA; 800-325-3010). Students learn in lecture and lab that an outstanding characteristic of antibodies is their property of specificity. The general hypothesis being tested is: a specific antigen will react with a specific antibody that has been formed against it, and form a precipitate. Therefore, horse albumin should react only with anti-horse serum, pig albumin with anti-pig, and so on. The results can be used to support the current classification of the ungulates at the order and suborder level. To accomplish this, students must become informed about the following subjects:
Adaptation of H9N2 Influenza Viruses to Mammalian Hosts: A Review of Molecular Markers

As the number of human infections with avian and swine influenza viruses continues to rise, the pandemic risk posed by zoonotic influenza viruses cannot be underestimated. Implementation of global pandemic preparedness efforts has largely focused on H5 and H7 avian influenza viruses; however, the pandemic threat posed by other subtypes of avian influenza viruses, especially the H9 subtype, should not be overlooked. In this review, we summarize the literature pertaining to the emergence, prevalence and risk assessment of H9N2 viruses, and add new molecular analyses of key mammalian adaptation markers in the hemagglutinin and polymerase proteins. Available evidence has demonstrated that H9N2 viruses within the Eurasian lineage continue to evolve, leading to the emergence of viruses with an enhanced receptor binding preference for human-like receptors and heightened polymerase activity in mammalian cells. Furthermore, the increased prevalence of certain mammalian adaptation markers and the enhanced transmissibility of selected viruses in mammalian animal models add to the pandemic risk posed by this virus subtype. Continued surveillance of zoonotic H9N2 influenza viruses, inclusive of close genetic monitoring and phenotypic characterization in animal models, should be included in our pandemic preparedness efforts.
Contributions of innate immunity to allograft rejection and survival

Experimental clinical evidence has highlighted the importance of early posttransplantation events in determining allograft survival. Graft inflammation is initiated by activation of cellular and humoral components of innate immunity in an antigen-independent manner. Subsequently, the alloreactive response is driven by donor-specific T-cell–dependent adaptive immunity. A positive cross-talk between innate and adaptive responses governs the magnitude of the host-versus-graft response. This article reviews recent studies on the cellular and molecular pathways through which innate immunity may trigger allograft rejection.
An Integrated Solution for 3D Heritage Modeling Based on Videogrammetry and V-SLAM Technology

This paper presents an approach for 3D reconstruction of heritage scenes using a videogrammetric-based device. The system, based on two video cameras with different characteristics, uses a combination of visual simultaneous localization and mapping (SLAM) and photogrammetry technologies. VSLAM, together with a series of filtering algorithms, is used for the optimal selection of images and to guarantee that the user does not lose tracking during data acquisition in real time. The different photogrammetrically adapted tools in this device and for this type of handheld capture are explained. An evaluation of the device is carried out, including comparisons with the Faro Focus X 330 laser scanner, through three case studies in which multiple aspects are analyzed. We demonstrate that the proposed videogrammetric system is 17 times faster in capturing data than the laser scanner and that the post-processing of the system is fully automatic, but takes more time than the laser scanner in post-processing. It can also be seen that the accuracies of both systems and the generated textures are very similar. Our evaluation demonstrates the possibilities of considering the proposed system as a new professional-quality measurement instrument.
Segmenting Purple Rapeseed Leaves in the Field from UAV RGB Imagery Using Deep Learning as an Auxiliary Means for Nitrogen Stress Detection

Crop leaf purpling is a common phenotypic change when plants are subject to some biotic and abiotic stresses during their growth. The extraction of purple leaves can monitor crop stresses as an apparent trait and meanwhile contributes to crop phenotype analysis, monitoring, and yield estimation. Due to the complexity of the field environment as well as differences in size, shape, texture, and color gradation among the leaves, purple leaf segmentation is difficult. In this study, we used a U-Net model for segmenting purple rapeseed leaves during the seedling stage based on unmanned aerial vehicle (UAV) RGB imagery at the pixel level. With the limited spatial resolution of rapeseed images acquired by UAV and small object size, the input patch size was carefully selected. Experiments showed that the U-Net model with the patch size of 256 × 256 pixels obtained better and more stable results with a F-measure of 90.29% and an Intersection of Union (IoU) of 82.41%. To further explore the influence of image spatial resolution, we evaluated the performance of the U-Net model with different image resolutions and patch sizes. The U-Net model performed better compared with four other commonly used image segmentation approaches comprising support vector machine, random forest, HSeg, and SegNet. Moreover, regression analysis was performed between the purple rapeseed leaf ratios and the measured N content. The negative exponential model had a coefficient of determination (R²) of 0.858, thereby explaining much of the rapeseed leaf purpling in this study. This purple leaf phenotype could be an auxiliary means for monitoring crop growth status so that crops could be managed in a timely and effective manner when nitrogen stress occurs. Results demonstrate that the U-Net model is a robust method for purple rapeseed leaf segmentation and that the accurate segmentation of purple leaves provides a new method for crop nitrogen stress monitoring.
Trichinella spiralis-induced mastocytosis and erythropoiesis are simultaneously supported by a bipotent mast cell/erythrocyte precursor cell

Anti-helminth responses require robust type 2 cytokine production that simultaneously promotes worm expulsion and initiates the resolution of helminth-induced wounds and hemorrhaging. However, how infection-induced changes in hematopoiesis contribute to these seemingly distinct processes remains unknown. Recent studies have suggested the existence of a hematopoietic progenitor with dual mast cell-erythrocyte potential. Nonetheless, whether and how these progenitors contribute to host protection during an active infection remains to be defined. Here, we employed single cell RNA-sequencing and identified that the metabolic enzyme, carbonic anhydrase (Car) 1 marks a predefined bone marrow-resident hematopoietic progenitor cell (HPC) population. Next, we generated a Car1-reporter mouse model and found that Car1-GFP positive progenitors represent bipotent mast cell/erythrocyte precursors. Finally, we show that Car1-expressing HPCs simultaneously support mast cell and erythrocyte responses during Trichinella spiralis infection. Collectively, these data suggest that mast cell/erythrocyte precursors are mobilized to promote type 2 cytokine responses and alleviate helminth-induced blood loss, developmentally linking these processes. Collectively, these studies reveal unappreciated hematopoietic events initiated by the host to combat helminth parasites and provide insight into the evolutionary pressure that may have shaped the developmental relationship between mast cells and erythrocytes.
Growth differentiation factor 15 in adverse cardiac remodelling: from biomarker to causal player

Heart failure is a growing health issue as a negative consequence of improved survival upon myocardial infarction, unhealthy lifestyle, and the ageing of our population. The large and complex pathology underlying heart failure makes diagnosis and especially treatment very difficult. There is an urgent demand for discriminative biomarkers to aid disease management of heart failure. Studying cellular pathways and pathophysiological mechanisms contributing to disease initiation and progression is crucial for understanding the disease process and will aid to identification of novel biomarkers and potential therapeutic targets. Growth differentiation factor 15 (GDF15) is a proven valuable biomarker for different pathologies, including cancer, type 2 diabetes, and cardiovascular diseases. Although the prognostic value of GDF15 in heart failure is robust, the biological function of GDF15 in adverse cardiac remodelling is not fully understood. GDF15 is a distant member of the transforming growth factor‐β family and involved in various biological processes including inflammation, cell cycle, and apoptosis. However, more research is suggesting a role in fibrosis, hypertrophy, and endothelial dysfunction. As GDF15 is a pleiotropic protein, elucidating the exact role of GDF15 in complex disease processes has proven to be a challenge. In this review, we provide an overview of the role GDF15 plays in various intracellular and extracellular processes underlying heart failure, and we touch upon crucial points that need consideration before GDF15 can be integrated as a biomarker in standard care or when considering GDF15 for therapeutic intervention.
Staheliomyces (Phallales) visited by Trigona (Apidae): melittophily in spore dispersal of an Amazonian stinkhorn?

Fungi are an important group of organisms disregarded by many biologists perhaps because they are neither plants nor animals. Estimations range from 65,000 to up to 250,000 fungus species (Hawksworth et al. 1985). Their role in ecosystems is often not sufficiently appreciated. To give only one example: about half of the recognized beetle families feed primarily on fungi or plant material substantially altered by fungal enzymes (Lawrence 1989). Contrary to their secret underground life the fruiting bodies are exposed as most fungal spores are dispersed by wind. However, insects may also play an important role in spore dispersal, as in some parasites (e.g. Alexander 1987, Ingold 1953, Jennersten 1988, Roy 1993, Strobel & Matern 1982) and in the stinkhorns (Phallales). The fruiting bodies of Phallus impudicus L. ex Pers. and other taxa of Phallaceae and Clathraceae attract mainly saprophagous and coprophagous flies which feed on the slimy, fetid gleba (e.g. Andersson 1989, Parmenter 1951, Schremmer 1963). The spores pass the digestive tract obviously unharmed (Fulton 1889, Ingold 1953, Schremmer 1963). In addition to such internal spore transport and consecutive dispersal, portions of the spore mass may be dispersed externally by sticking to the flies' surface.