Miracle-dz/UniFAR-FTUs huggingface dataset:
LOCAL MALADAPTATION IN THE ANTHER‐SMUT FUNGUS MICROBOTRYUM VIOLACEUM TO ITS HOST PLANT SILENE LATIFOLIA: EVIDENCE FROM A CROSS‐INOCULATION EXPERIMENT

Conventional wisdom holds that parasites evolve more rapidly than their hosts and are therefore locally adapted, that is, better at exploiting sympatric than allopatric hosts. We studied local adaptation in the insect‐transmitted fungal pathogen Microbotryum violaceum and its host plant Silene latifolia. Infection success was tested in sympatric (local) and allopatric (foreign) combinations of pathogen and host from 14 natural populations from a metapopulation. Seedlings from up to 10 seed families from each population were exposed to sporidial suspensions from each of four fungal strains derived from the same population, from a near‐by population (< 10 km distance), and from two populations at an intermediate (< 30 km) and remote (< 170 km) distance, respectively. We obtained significant pathogen X plant interactions in infection success (proportion of diseased plants) at both fungal population and strain level. There was an overall pattern of local maladaptation of this pathogen: average fungal infection success was significantly lower on sympatric hosts (mean proportion of diseased plants = 0.32 ± 0.03 SE) than on allopatric hosts (0.40 ± 0.02). Five of the 14 fungal populations showed no strong reduction in infection success on sympatric hosts, and three even tended to perform better on sympatric hosts. This pattern is consistent with models of time‐lagged cycles predicting patterns of local adaptation in host‐parasite systems to emerge only on average. Several factors may restrict the evolutionary potential of this pathogen relative to that of its host. First, a predominantly selfing breeding system may limit its ability to generate new virulence types by sexual recombination, whereas the obligately outcrossing host 5. latifolia may profit from rearrangement of resistance alleles by random mating. Second, populations often harbor only a few infected individuals, so virulence variation may be further reduced by drift. Third, migration rates among host plant populations are much higher than among pathogen populations, possibly because pollinators prefer healthy over diseased plants. Migration among partly isolated populations may therefore introduce novel host plant resistance variants more often than novel parasite virulence variants. That migration contributes to the coevolutionary dynamics in this system is supported by the geographic pattern of infectivity. Infection success increased over the first 10–km range of host‐pathogen population distances, which is likely the natural range of gene exchange.
DYNAMIC AND GENETIC CONSEQUENCES OF VARIATION IN HORIZONTAL TRANSMISSION FOR A MICROPARASITIC INFECTION

Transmission to a new host is a critical step in the life cycle of a parasite. Variation in the characteristics of the transmission process, for example, due to host demography, is assumed to select for different variants of the parasite. We have experimentally tested how variation in the time to transmission (early or late after infection) and exposure to adverse conditions outside the host (immediate or delayed contact with new host) interact to determine the success of the infection in the next host, using the trypanosome Crithidia bombi infecting its bumblebee host, Bombus terrestris. These two experimentally manageable steps mimic the processes of within‐ and among‐host selection for the parasite. We found that early transmission led to higher infection success in the next host as did immediate contact with the new host. However, there was no interaction between the two parameters as would be expected if early‐transmitted variants, resulting from rapid multiplication within the host, would be less adapted to the conditions encountered during the between‐host transfer or infection of the next host. Furthermore, typing the genetic variability of the parasites with microsatellites showed that the four different transmission routes of our experiment selected for different degrees of allelic diversity of the infecting parasite populations. The results support the idea that variation in the transmission process selects for different genotypic variants of the parasite. At the same time, the relationship of allelic diversity with infection intensity suggested that the coinfection model of May and Nowak (1995) may be appropriate, where each parasite is able to infect and multiply independent of others within the same host.
Vitamin D and muscle trophicity

Purpose of reviewWe review recent findings on the involvement of vitamin D in skeletal muscle trophicity. Recent findingsVitamin D deficiencies are associated with reduced muscle mass and strength, and its supplementation seems effective to improve these parameters in vitamin D-deficient study participants. Latest investigations have also evidenced that vitamin D is essential in muscle development and repair. In particular, it modulates skeletal muscle cell proliferation and differentiation. However, discrepancies still exist about an enhancement or a decrease of muscle proliferation and differentiation by the vitamin D. Recently, it has been demonstrated that vitamin D influences skeletal muscle cell metabolism as it seems to regulate protein synthesis and mitochondrial function. Finally, apart from its genomic and nongenomic effects, recent investigations have demonstrated a genetic contribution of vitamin D to muscle functioning. SummaryRecent studies support the importance of vitamin D in muscle health, and the impact of its deficiency in regard to muscle mass and function. These ‘trophic’ properties are of particular importance for some specific populations such as elderly persons and athletes, and in situations of loss of muscle mass or function, particularly in the context of chronic diseases.
PATTERNS OF DESCENT IN CLONAL LINEAGES AND THEIR MULTILOCUS FINGERPRINTS ARE RESOLVED WITH COMBINED GENE GENEALOGIES

Clonal lineages in the filamentous ascomycete (fungi) Sclerotinia sclerotiorum were determined by analysis of genealogies of four loci: the intergenic spacer of the nuclear ribosomal repeat (IGS; approximately 4 kb), the translation elongation factor (EF‐1α; approximately 300 bp), an anonymous region (44.11; approximately 700 bp), and the calmodulin gene (CAL; approximately 400 bp). Three of the four loci are physically unlinked. The combined analysis of the four loci provided the best estimate of phylogeny, which is consistent with a pattern of some recombination among clonal lineages against a background of predominant clonality. Comparison of gene genealogies with a phylogeny inferred from DNA fingerprints and a combined phylogeny of the entire dataset identified convergent or parallel changes in fingerprints. Analysis of the entire data matrix allowed us to resolve patterns of descent among clonal lineages that could not be inferred from fingerprints alone and to discern recent episodes of divergence that were not detected in gene genealogies. Prerequisites for applying this approach to other systems are a haploid context for inferring multiple gene genealogies (such as the mitochondrial genome) that indicate limited recombination and another data matrix that identifies recently evolved genotypes.
Ácido linoleico conjugado: interés actual en nutrición humana

El termino acido linoleico conjugado (ALC) incluye una serie de isomeros posicionales y geometricos del acido linoleico. Las principales fuentes naturales son la carne de animales rumiantes, la leche y los derivados lacteos. Ademas, existe en el mercado una amplia gama de alimentos que han sido enriquecidos en ALC (manipulacion de la dieta del animal, adicion de ALC procedente de otras fuentes naturals como el aceite de cartamo). La diferencia estriba en que, mientras el principal isomero presente de forma natural en los alimentos es el cis-9 , trans-11 , las preparaciones comerciales contienen cantidades similares de los isomeros cis-9 , trans-11 y trans-10 , cis-12 . Aunque el ALC es un componente minoritario en la dieta, en la actualidad had espertado gran interes y ha promovido una intensa investigacion acompanada de un boom publicitario debido a sus potenciales efectos sobre la salud: modificacion de la composicion corporal con reduccion de la masa grasa, propiedades antiaterogenicas, hipolipemiantes, antidiabetogenicas e inmunomoduladoras, entre otras. Estos efectos se han observado principalmente en modelos animales y cultivos celulares. Los estudios en humanos son escasos e incluso, a veces, contradictorios. En esta revision se profundiza en los trabajos de investigacion mas recientes sobre el interes nutricional del ALC. De la amplia revision realizada se deduce la necesidad de estudios adicionales en humanos que permitan obtener datos sobre las cantidades optimas de ingesta, asi como sobre los efectos tanto a corto como a largo plazo, y posibles efectos secundarios de cada isomero individual del ALC con el fin de determinar su seguridad y eficacia.
CORRELATED TROPHIC SPECIALIZATION AND GENETIC DIVERGENCE IN SYMPATRIC LAKE WHITEFISH ECOTYPES (COREGONUS CLUPEAFORMIS): SUPPORT FOR THE ECOLOGICAL SPECIATION HYPOTHESIS

There is ample empirical evidence that phenotypic diversification in an adaptive radiation is the outcome of divergent natural selection related to differential resource use. In contrast, the role of ecological forces in favoring and maintaining reproductive isolation in nature remains poorly understood. If the same forces driving phenotypic divergence are also responsible for speciation, one would predict a correlation between the extent of trophic specialization (reflecting variable intensity of divergent natural selection) and that of reproductive isolation being reached in a given environment. We tested this hypothesis by comparing the extent of morphological and genetic differentiation between sympatric dwarf and normal whitefish ecotypes (Coregonus sp.) from six lakes of the St. John River basin (eastern Canada and northern Maine). Eight meristic variables, 19 morphometric variables, and six microsatellite loci were used to quantify morphological and genetic differentiation, respectively. Dwarf and normal ecotypes in each lake differed primarily by traits related to trophic specialization, but the extent of differentiation varied among lakes. Significant but variable genetic divergence between ecotypes within lakes was also observed. A negative correlation was observed between the extent of gene flow between ecotypes within a lake and that of their morphological differentiation in trophic‐related traits. The extent of reproductive isolation reached between dwarf and normal whitefish ecotypes appears to be driven by the potential for occupying distinct trophic niches and, thus, by the same selective forces driving tropic specialization in each lake. These results therefore support the hypothesis of ecological speciation.
Inhibitors of cannabinoid receptors and glucose metabolism

Purpose of reviewAbdominal obesity is closely related to type 2 diabetes and overactivity of the endocannabinoid system. The present review aims at evaluating the role of endocannabinoid system in glucose dysregulation and the effects of cannabinoid 1 receptor blockade on glucose metabolism in both animal models and overweight/obese humans, especially with type 2 diabetes. Recent findingsCannabinoid 1 receptors have been identified not only in the brain, but also in the adipose tissue, the gut, the liver, the skeletal muscle and even the pancreas, all organs playing a key role in glucose metabolism and type 2 diabetes. Rimonabant, the first selective cannabinoid 1 receptor blocker in clinical use, has been shown to reduce body weight, waist circumference, glycated haemoglobin, triglycerides, insulin resistance index, and to increase HDL cholesterol and adiponectin concentrations in patients with type 2 diabetes, confirming data on nondiabetic overweight/obese patients. Almost half of the metabolic changes, including glycated haemoglobin reduction, could not be explained by weight loss, in agreement with direct peripheral effects. SummaryCannabinoid 1 blockade reduces food intake and body weight and improves metabolic regulation beyond just weight loss. Because of its positive effect on glucose metabolism, rimonabant deserves consideration in the treatment of overweight/obese patients with type 2 diabetes.
Measuring muscle blood flow: a key link between systemic and regional metabolism

Purpose of reviewTo provide a brief overview of the main techniques to measure muscle blood flow in humans and highlight some of the strengths and weaknesses associated with each technique. Recent findingsPeak muscle blood flow values of 300 ml/min per 100 g are possible in humans during heavy exercise performed with small muscle mass. This value is far higher than that which appears in most textbooks. Accurate and reliable techniques are therefore essential in measuring muscle blood flow. Current invasive techniques commonly used include indicator dilution (thermodilution and dye dilution) and radiolabel tracer washout (e.g. 133Xe washout) methods. Although invasive techniques have provided valuable insight into tissue blood flow, noninvasive techniques such as venous occlusion plethysmography and Doppler ultrasound are frequently used and provide accurate measurements of blood flow. Newer imaging techniques (MRI, positron emission tomography, and contrast-enhanced ultrasonography) promise increased resolution of measurements of local blood flow, including in discrete tissues in which more classical techniques are not able to be used. SummaryMuscle blood flow is a key link in the interplay and regulation of systemic and local muscle metabolism. Recognizing the advantages and limitations of each technique is essential to translational researchers studying the effects of nutrition and metabolism on muscle blood flow.
‘Just Helping’: Children living with a parent with young onset dementia

Objectives: While the consequences of caring for younger people with dementia have been a growing area of research, little is known about the children of these individuals. This study aimed to discover whether children of younger people with dementia can be compared to other young carers, the impact of their caring on mood, burden and resilience and what could promote coping. Method: In-depth interviews were carried out with 12 participants aged 11–18. A grounded theory methodology was used, supplemented with three quantitative measures. Results: Four higher order categories were identified focusing on: discovering dementia; developing a new relationship; learning to live with it and going through it together. Few participants showed depressive symptomatology, but more than half showed high levels of burden and most showed moderate levels of resilience. A three-stage process model of adapting to dementia is proposed, with children moving through grief to emotional detachment and increased maturity. Conclusion: This study has implications for service development in light of the National Dementia Strategy and proposals for whole family approaches for young carers. Whole family working by both dementia and children's services could reduce the burden on children of people with young onset dementia.
Fixation Probabilities Depend on Life History: Fecundity, Generation Time and Survival in a Burst-Death Model

Abstract The burst-death model has been developed to describe the life history of organisms with variable generation times and a burst of a fixed number of offspring. The model also includes an optional constant clearance rate, such as washout from a chemostat, and the possibility of sustained periods of population growth followed by severe bottlenecks, as in serial passaging. In this model, a beneficial mutation can either increase the burst rate or the burst size, or reduce the clearance rate, thus increasing survival. In this article we examine the effects of these three possible mechanisms on both the Malthusian fitness and the fixation probability of the lineage. We find that equivalent relative increases in the burst rate or burst size confer equivalent increases in the Malthusian fitness of a lineage, whereas increasing survival typically has a more moderate effect on Malthusian fitness. In contrast, for beneficial mutations that confer the same increase in fitness, mutations that increase survival are the most likely to fix, followed by mutations that increase the burst rate. Mutations that increase the burst size are the least likely to fix. These results imply that mutant lineages with the highest Malthusian fitness are not, in many cases, the most likely to escape extinction.
Socially Acquired Host-Specific Mimicry and the Evolution of Host Races in Horsfield'S Bronze-Cuckoo Chalcites Basalis

Abstract Coevolution between parasites and their hosts typically leads to increasing specialization on host species by the parasite. Where multiple hosts are parasitized, specialization on each host can result in genetic divergence within the parasite population to create host races, and, ultimately, new species. We investigate how host-specific traits arise in Horsfield's bronze-cuckoo Chalcites basalis nestlings. Newly hatched cuckoos evict host young from the nest, yet in the absence of a model they accurately mimic the different begging calls of a primary host (superb fairy-wren, Malurus cyaneus) and a secondary host (buff-rumped thornbill, Acanthiza reguloides). Using cross-fostering experiments, we show that begging calls are modified after parasitism, through experience. Further, we demonstrate the mechanism by which mimetic calls are acquired. All cuckoo nestlings initially produced the call of their primary host. When cross-fostered as eggs to a secondary host, calls increased in variability and were rapidly modified to resemble those of the secondary host through shaping by host parents. We suggest that plasticity in the development of host-specific traits after parasitism is likely to reduce selection for host race formation.
Fatty acids and postprandial inflammation

Purpose of reviewPostprandial inflammation is an independent factor in evaluating food quality in addition to the well known parameters of nutritional value, caloric content and amount of carbohydrates, fats, proteins, minerals and vitamins. Among the latter, the quality and quantity of fatty acids in a meal is a major determinant of the magnitude of postprandial inflammation. Purpose of this review is to describe this exciting new area of research and its repercussions in the way we, the consumers, and the food industry evaluate the type and quantity of fat in food. Recent findingsA number of, by now classical, epidemiologic studies have documented a strong association between the type of fatty acids consumed and the incidence of cardiovascular diseases. Recently published reports suggest that the adverse effect of dietary fatty acids on cardiovascular health depends on their postprandial modification of innate immunity ending in the so-called ‘postprandial metabolic inflammation’. SummaryThe quantity of fat and its qualitative characteristics such as the percentage of saturated fatty acids and the ratio of n-3 to n-6 polyunsaturated fatty acids (PUFAs) in a meal have emerged as major determinants of the magnitude of postprandial inflammatory response. In this review, we will summarize all experimental evidence suggesting that the two families of PUFA appear to have antagonistic effects on postprandial inflammation, n-3 PUFA being anti-inflammatory while n-6 PUFA proinflammatory.
Patterns of Endemism and Species Richness in Malagasy Cophyline Frogs Support a Key Role of Mountainous Areas for Speciation

Abstract Cophyline narrow-mouthed frogs (Anura: Microhylidae) are a diverse endemic radiation of Madagascar. Cophylines contain a high proportion of range restricted species and constitute a good model system to understand patterns of evolutionary diversification in tropical ecosystems. We combine spatial and phylogenetic analyses for a near-complete taxon sample to test competing explanations for patterns of species richness (SR) and endemism. Our reconstruction of the phylogeny of cophylines indicates the presence of 22 new species and several instances of nonmonophyly. We found a strong historical signal in current cophyline ranges indicating a high degree of spatial niche conservatism in clade diversification, with clades occurring in the North of Madagascar constituting the most derived in the phylogeny. We identified six positively correlated centers of SR and endemism that can neither be explained by stochastic models such as elevational or latitudinal mid-domain effect, nor by low-elevation river catchments. Instead, the locations of these centers in areas spanning a high altitudinal range in combination with specific climatic parameters support a key role of mountainous areas for speciation of these anurans, although we cannot exclude an influence of habitat loss due to human impact. High conservation priority is ascribed to these areas.
Elderly people who committed suicide – their contact with the health service. What did they expect, and what did they get?

Objective: Contact between elderly patients and healthcare professionals may be crucial for suicide prevention if suicidal tendencies are revealed and help is to be provided. The objective of the study was to investigate how elderly suicide cases had perceived the health service and what characterised their contact with it. Method: This is a psychological autopsy study based on qualitative interviews with people who had known 1 of the total of 23 suicide cases aged over 65. The 63 informants were relatives, general practitioners (GPs) and home-based care workers. The systematic text condensation method was applied to analyse interviews. Results: Many of the elderly expressed distrust of health service once their functional decline began. They feared losing their autonomy if they became dependent on help, and many therefore refused health service provisions. Communication between them and helpers failed. As they gradually became more dependent on medical care, many experienced that they were not given the desired help, which confirmed their distrust. Conclusion: Contact between these people and the health service must inspire confidence for it to prevent suicide. Elderly people at risk of suicide are vulnerable: they feel degraded if their autonomy is threatened by health personnel. The structure and organisation of the health service, and each worker's contact with the elderly, must preserve their dignity. Dignity must be evinced through the healthcare professionals’ treatment of elderly people and a system that meets their needs.
A systematic analysis of genetic dilated cardiomyopathy reveals numerous ubiquitously expressed and muscle‐specific genes

Despite considerable progress being made in genetic diagnostics for dilated cardiomyopathy (DCM) using panels of the most prevalent genes, the cause remains unsolved in a substantial percentage of patients. We hypothesize that several previously described DCM genes with low or unknown prevalence have been neglected, which, if catalogued, could increase the yield of diagnostic DCM testing. The aim of this study is to catalogue all genetic evidence on DCM comprehensively.