Miracle-dz/UniFAR-FTUs huggingface dataset:
Cloning and Expression of Nonstructural Protein NS1 of Dengue Virus Serotype 2

Early diagnosis of dengue virus (DENV) infection is affirmative for patient management and control of the disease. Detection of nonstructural-1 (NS1) antigen has been proven to provide early detection of DENV infection. Commercial NS1 antigen assays are available in Indonesia with variable sensitivity. In an attempt to develop an NS1-based diagnostic test, we successfully cloned NS1 gene of DENV2 to a glutathione Stransferase- based vector pGEX6P-1 in Escherichia coli system. The recombinant protein (pG2NS12) was expressed in E. coli BL21. After induction with isopropyl-β-D-thiogalactoside 0.1 mM for 4 h at 25 °C a recombinant protein GST-NS1 with molecular size of approximately 75 kDa was obtained. The fusion protein was insoluble and found in the pellet fraction of the cell lysate. Addition of lysozyme (10 mg mL -1 ) and DNase-I (7.2 mg mL -1 ) in the lysis buffer was necessary to collect proteins from the pellet fraction. The proteins in the cell pellet were fractionated through Sephadex-G100 column, and GST-NS1 was further purified with Glutathione-Sepharose 4B beads. To obtain pure recombinant NS1 protein to be used in the immunization of mice, the fusion protein was cut with PreScission Protease® by addition of 0.075% Triton-X 100 was necessary to cut the fusion protein. We found that antibodies that recognized the recombinant NS1 protein and DENV2 virus were produced in mice immunized with purified NS1 protein. Therefore, our recombinant NS1 could be used to produce antibody that is potentially useful for developing diagnostic assay to determine the presence of dengue virus NS1 antigen in patient sera.
A FLEXIBLE STATE SPACE MODEL AND ITS APPLICATIONS

The standard state space model treats observations as imprecise measurement of the Markovian states. Our flexible model handles the states and observations symmetrically, which are simultaneously determined by past observations and up to first‐lagged states. The only distinction between the states and observations is the observability. When it is applied to the autoregressive moving average, dynamic factor and stochastic volatility models, the state space form is both parsimonious and intuitive, for low‐dimension states are constructed simply by stacking all the relevant but unobserved components in the structural model.
True Metabolisable Energy and True Amino Acid Availability in Chinese Varieties of Dehulled and Hulled Soybean Meals Determined with Adult Roosters

Studies were conducted with intact White Leghorn roosters to determine the true metabolisable energy (TME) and the true amino acid availability (TAAA) in five dehulled and hulled soybean meals produced in China. 60 roosters, kept in individual cages, were fasted for 48 h and then tube-fed 50 g of one of experimental feedstuffs and their excreta was then collected for the subsequent 48 h period. Two separate collection periods were used with each meal being fed to 12 roosters. The birds were given a 15-day recovery period between collection periods. An additional 12 roosters were either fasted or fed a protein-free diet in order to estimate the extent of endogenous losses of energy and amino acids in excreta. The average values per bird for nitrogen loss, endogenous energy losses (EEL) and endogenous energy losses corrected to zero-nitrogen balance (EELn) were found to be 0.74 g, 47.0 kJ and 21.5 kJ, respectively. It was found that the TME and TAAA values of dehulled soybean meal were higher than those of hulled soybean meal. The TME and nitrogen-corrected TME metabolisable energy values of dehulled soybean meal were 10.58 and 10.74 MJ/kg, respectively, while the corresponding values for hulled soybean meal were 10.03 and 10.27 MJ/kg, respectively. The average indispensable and dispensable amino acid availability of dehulled soybean meal was 92.1 and 93.5%, compared with 89.3 and 91.4% for hulled soybean meal. Dehulled soybean meal would therefore appear to be superior to hulled soybean meal as a source of protein and energy for use in poultry rations. (Asian-Aust. J. Anim. Sci. 2003. Vol 16, No. 10 : 1487-1494)
Sensitivity to Meter in Auditory Feedback During Music Performance

A long-standing question in research on the role of auditory feedback during production (including music performance) concerns the role of feedback content, which in music refers to pitch categories. Whereas Howell and colleagues claimed that producers are only sensitive to rhythmic synchronization between actions and sound (Howell, Powell, & Khan, 1983), serial shifts of pitch content (e.g., hearing the previous planned pitch at each keypress) disrupt piano performance (Pfordresher, 2005). However, some results suggest that the basis of disruption from altered pitches may relate to higher-order rhythms conveyed by the pattern of melodic accents in auditory feedback that communicate meter (cf. Jones, 1987). Thus, we tested whether participants would be disrupted while hearing a feedback sequence with a conflicting meter. In 2 experiments, participants performed either binary or ternary meter melodies while hearing auditory feedback that could be altered with respect to its metrical organization (thus forming a different pitch sequence), and/or its sequential alignment with the planned melody. Auditory feedback with conflicting metrical accents disrupted performance as did serial shifts of the planned melody. Thus, the disruptive effect of altered pitch may reflect rhythmic organization, albeit in a different sense than was suggested by Howell and colleagues.
A Proposal for a Machine Learning Classifier for Viral Infection in Living Cells Based on Mitochondrial Distribution

The study of viral infections using live cell imaging (LCI) is an important area with multiple opportunities for new developments in computational cell biology. Here, this point is illustrated by the analysis of the sub-cellular distribution of mitochon‐ drium in cell cultures infected by Dengue virus (DENV) and in uninfected cell cul‐ tures (Mock-infections). Several videos were recorded from the overnight experiments performed in a confocal microscopy of spinning disk. The density dis‐ tribution of mitochondrium around the nuclei as a function of time and space ρ(r , θ, t) was numerically modeled as a smooth interpolation function from the image data and used in further analysis. A graphical study shows that the behavior of the mitochondrial density is substantially different when the infection is present. The DENV-infected cells show a more diffuse distribution and a stronger angular variation on it. This behavior can be quantified by using some usual image process‐ ing descriptors called entropy and uniformity. Interestingly, the marked difference found in the mitochondria density distribution for mock and for infected cell is present in every frame and not an evidence of time dependence was found, which indicate that from the start of the infections the cells are showing an altered subcel‐ lular pattern in mitochondrium distribution. Ulteriorly, it would be important to study by analysis of time series for clearing if there is some tendency or approxi‐ mate cycles. Those findings are suggesting that using the image descriptors entropy and uniformity it is possible to create a machine learning classifier that could recog‐ nize if a single selected cell in a culture has been infected or not.
HIghMass—HIGH H i MASS, H i-RICH GALAXIES AT z ∼ 0 HIGH-RESOLUTION VLA IMAGING OF UGC 9037 AND UGC 12506

We present resolved H i observations of two galaxies, UGC 9037 and UGC 12506, members of a rare subset of galaxies detected by the ALFALFA extragalactic H i survey characterized by high H i mass and high gas fraction for their stellar masses. Both of these galaxies have M* > 1010 M☉ and M*, as well as typical star formation rates for their stellar masses. How can such galaxies have avoided consuming their massive gas reservoirs? From gas kinematics, stability, star formation, and dark matter distributions of the two galaxies, we infer two radically different histories. UGC 9037 has high central H i surface density (>10 M☉ pc−2). Its gas at most radii appears to be marginally unstable with non-circular flows across the disk. These properties are consistent with UGC 9037 having recently acquired its gas and that it will soon undergo major star formation. UGC 12506 has low surface densities of H i, and its gas is stable over most of the disk. We predict its gas to be H i-dominated at all except the smallest radii. We claim a very high dark matter halo spin parameter for UGC 12506 (λ = 0.15), suggesting that its gas is older, and has never undergone a period of star formation significant enough to consume the bulk of its gas.
1 Biomarkers in asthma and allergic rhinitis

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Biomarkers in asthma and allergic rhinitis Z. Diamant, J.D. Boot, E. Mantzouranis, R. Flohr, P.J. Sterk, R. Gerth van Wijk
Simulation and Optimization of Saponification of Ethyl Acetate in the Presence of Sodium Hydroxide in a Plug Flow Reactor Using Aspen Plus

This work presents the modelling and simulation of saponification of ethyl acetate in the presence of sodium hydroxide in a plug flow reactor using Aspen Plus simulation software. Plug flow reactors are widely used in the industry due to the non-mixing property. The use of plug flow reactors becomes significant when there is a need for continuous large scale reaction or fast reaction. Plug flow reactors have a high volumetric unit conversion as the occurrence for side reactions is minimum. In this research Aspen Plus V8.0 has been successfully used to simulate the plug flow reactor. In order to simulate the process as accurately as possible HYSYS Peng-Robinson EOS package was used as the property method. The results obtained from the simulation were verified by the experiment carried out in the EDIBON plug flow reactor module. The correlation coefficient (r2) was 0.98 and it proved that simulation results satisfactorily fit for the experimental model. The developed model can be used as a guide for understanding the reaction kinetics of a plug flow reactor.
iii COMPARISON OF PLATE MEDIA FOR ISOLATION OF CAMPYLOBACTER FROM LIVE BROILERS AND SCHEDULED DELIVERY OF BROILER FLOCKS TO REDUCE CROSS-CONTAMINATION WITH CAMPYLOBACTER

Several selective media containing a variety of different antibiotic supplements are available for culturing thermophilic enteropathogenic campylobacters from live broilers. In the present work, we compared the efficiency of different plate media in two different experiments. In our first study eighty birds were placed in four batteries (groups A, B, C and D). The birds were challenged in drinking water with 10 CFU of C. jejuni per ml. Three birds per group were killed with CO2 on day 8, 15, 21, 28 and 35 their ceca were removed, weighed and serially diluted in phosphate buffer solution (PBS). Birds in treatment D were gavaged with PBS containing gentamicin sulfate at different concentrations. Dilutions were plated onto BAP (Bap-B), Bap-H, modified charcoal cefoperazone deoxycholate (mCCDA), Campy-Line (CL) and modified Campy-Cefex (mCC) agar plates. In our second study, 180 fecal samples were collected in six independent trips, in which three houses from three different farms were visited per trip (total = 18 houses/farms, 10 samples per house). The samples were pooled and three pooled samples per house were tested for Campylobacter. Cecal samples were also collected in two out of the six trips (5 samples per house). Both fecal and cecal samples
Effect of Oxygen Therapy by Venturi Mask versus Non Invasive Ventillation on the Outcome of Patients Who Devolope Hypoxia after Open Heart Surgery

Background: Cardiothoracic surgery is a common cause of acute respiratory failure and is associated with increased morbidity and mortality. We aimed to compare the outcomes of open heart surgery patients treated using oxygen delivery devices with patients who receive NIV as a first-line therapy for hypoxemic respiratory failure. Materials and Methods: 40 patients who developed acute hypoxemic respiratory failure after open heart surgery and admitted to cardiothoracic ICU 20 patients received NIV and 20 patient received oxygen by venture mask. For all patients the following measurements were performed before and after CPAP AND Venture use: CBC, blood urea, serum creatinine body temperature, chest X-ray, Arterial blood gases (arterial pH, sodium bicarbonate, pcO2, SpO2 and PaO2-to-FiO2 ratio). Results: Mean PO2 and SO2 have increased after using of both venture and Cpap, increase in both PCO2 and HCO3 levels after using Venturi mask, CPAP mask was superior to venturi mask in avoiding the need of intubation, decreasing The ICU stay median length and also median length of hospitalization, all were lower in CPAP group than venture group. Also the mortality rate was lower in CPAP group than the venturi group. Conclusion: Using CPAP mask in severe AHRF following open heart surgery can avoid intubation, decreases the levels of tachypnea and arterial hypoxemia, decreases ICU stay, the length of hospitalization and also decreases the mortality rate compared with patients receiving high-concentration oxygen therapy with venture mask.
04 AGGRESSIVE AND PEACEFUL BEHAVIOR IN MULTIAGENT SYSTEMS ON CELLULAR SPACE

One of the key issues in Multi-Agent simulation approach is a consolidation of great model variety. Many researches govern own unique models that are similar in basic principles but for complex adaptive systems such as Artificial Ecosystems slight difference in architecture and parameters calibration could affect crucially on the emergent properties of the model. As it was denoted by the pioneers of the Artificial Ecosystems modelling Robert Axtell and Robert Axelrod: variety of Multi-Agent models need introduction of methods and technics that allows consolidating of its results. In work we present modification of model similar to classic Artificial Life spatial lattice models and trace the exhibition of aggressive and peaceful behavior depending on the income resource. We consider results of both models’ simulation as it was proposed in «docking models» method by Axtell and Axelrod.
34 Physical Object Repre sen ta tions for Perception and Cognition

How might we explain these flexible, seemingly effortless judgments? This chapter pre sents an answer centered at the notion of physical object repre sen tations (PORs), a basic system of knowledge that supports perceiving, learning, and reasoning about all the objects in our environment— their shapes, appearances, affordances, substances, and the way they react to forces applied to them. Our goal here is to outline a computational framework for studying the form and content of PORs in the mind and brain. PORs can be considered an interface between perception and cognition, linking what we perceive to how we plan our actions and talk about the world. Despite their fundamental role in perception, many impor tant questions about object repre sen ta tions remain open. What kind of information formats or data structures underlie PORs so as to support the many ways in which humans flexibly and creatively interact with the world? How can properties of objects be inferred from sensory inputs, and how are they represented in neural cir cuits? How can these repre sen ta tions integrate sense data across vision, touch, and audition? After introducing the computational ingredients of POR theory from a reverseengineering perspective, we review recent work that is beginning to answer some of these questions. We focus on three case studies: (1) how PORs can explain human judgments in intuitive physics, across a broad range of physical outcome prediction scenarios; (2) how PORs provide a substrate for physically mediated object shape perception in scenarios where traditional visual cues fail and a natu ral substrate for multimodal (visualhaptic) perception and crossmodal transfer; and (3) how in one domain of highlevel vision— face perception— PORs might be computed by neural cir cuits, and how thinking in terms of PORs suggests a new way to interpret multiple stages of pro cessing in the primate brain.
Microsurgical Management of Intraspinal Tumors in the Lumbar Spine: Hemilaminectomy as an Alternative to Laminectomy

Introduction: Laminectomy is the classical surgical technique done for exposure of the spinal cord in the removal of intraspinal lesions. Here, we report a case where multilevel hemilaminectomy has been done to remove a long segment intraspinal tumor extending from D11 to L5. Case report: A 47-year-old lady who had backache for 5 years, difficulty in walking for 6 month. She came to us with paraplegia (grade 0 power of both lower limbs), decreased sensation of both lower limbs and bladder incontinence. MRI revealed an intradural mass lesion extending from D11 to L5 level. Multilevel hemilaminectomy was performed from D11 to L5 and near total resection of the tumor was done. Postoperatively, patient showed improvement with grade 1-2 power in lower limbs at the end of 3 months. Advantages of hemilaminectomy: The main advantage of hemilaminectomy is the complete preservation of dorsal static structures of the vertebral column; such as the spinous process, the interspinous and supraspinous ligaments, and the unilateral preservation of the intervertebral joints, laminae, ligamentum flavae and the paraspinal muscles. Conclusion: It is without doubt that patient morbidity and spine stability is better when surgery is performed through a narrow corridor. It can be concluded that hemilaminectomy is a safe and better alternative to laminectomy in the removal of long segment intradural tumors.
Evolution of cosmic magnetic fields: From the very early Universe, to recombination, to the present

A detailed numerical and analytical examination of the evolution of stochastic magnetic fields between a putative magnetogenesis era at high cosmic temperatures T{approx}100 MeV-100 GeV and the present epoch is presented. The analysis includes all relevant dissipation processes, such as neutrino- and photon-induced fluid viscosities as well as ambipolar and hydrogen diffusion. A simple and intuitive analytical model matching the results of the three-dimensional MHD simulations allows for the prediction of prerecombination and present day magnetic field correlation lengths and energy densities as a function of initial magnetic field energy density, helicity, and spectral index. Our conclusions are multifold. (a) Initial primordial fields with only a small amount of helicity are evolving into maximally helical fields at the present. Furthermore, the simulations show a self-similarity in the evolution of maximally helical fields implying a seemingly acausual amplification of magnetic fields on large scales is observed. (b) There exists a correlation between the strength of the magnetic field B at the peak of its spectrum and the location of the peak, given at the present epoch by B{approx_equal}5x10{sup -12} G(L/kpc), where L is the magnetic field correlation length determined by the initial properties of the magnetic field. (c) Concerning studies ofmore » the generation of cosmic microwave background (CMBR) anisotropies due to primordial magnetic fields of B{approx}10{sup -9} G on > or approx. 10 Mpc scales, such fields are not only impossible to generate in early causal magnetogenesis scenarios but also seemingly ruled out by distortions of the CMBR spectrum due to magnetic field dissipation on smaller scales and the overproduction of cluster-magnetic fields. (d) The most promising detection possibility of CMBR distortions due to primordial magnetic fields may be on much smaller scales at higher multipoles l{approx}10{sup 6} where the signal is predicted to be the strongest. (e) It seems possible that magnetic fields in clusters of galaxies are entirely of primordial origin, without invoking dynamo amplification. Such fields would be of (precollapse) strength 10{sup -12}-10{sup -11} G with correlation lengths in the kpc range and would also exist in voids of galaxies. « less
Arrhythmic Risk in Long-QT Syndrome Polymorphism Acting as a Protective Modifier Against KCNQ 1 Identification of a

Horie, Swan, Lauri Toivonen, Annukka M. Lahtinen, Kimmo Kontula, Wataru Shimizu, Minoru Federica Dagradi, Alessandro Vicentini, Didier Klug, Paul A. Brink, Althea Goosen, Heikki Seiko Ohno, Véronique Fressart, Maria Cristina Monti, Cristina Crocamo, Matteo Pedrazzini, Sabine Duchatelet, Lia Crotti, Rachel A. Peat, Isabelle Denjoy, Hideki Itoh, Myriam Berthet, Arrhythmic Risk in Long-QT Syndrome Polymorphism Acting as a Protective Modifier Against KCNQ1 Identification of a
1 Anesthetic complications — how bad things happen

The delivery of anesthesia in any setting is not without risk. The environment is complex, uncertain, and ever-changing. Human performance of this potentially hazardous task can be unpredictable and imperfect, especially in times of urgency, intensity, and time pressure. Risk and human error cannot be eliminated, but can be reduced and managed by eliminating a culture of blame and punishment and replacing it with a culture of vigilance and cooperation to expose and remediate system weaknesses, which, in combination, often lead to error and injury. The concept is straightforward. Most patients do not enjoy going to the dentist. Although patients understand that pain can be eliminated with local anesthesia, fear and anxiety still fuel avoidance of necessary care. Dentistry has responded to these issues by providing options for various levels of sedation, analgesia, or general anesthesia in the dental office. Usually, all goes well. Patients are satisfied; necessary dental work gets done. Sometimes, however, things do not go well. Complications (adverse events, sentinel events) are defined as unplanned, unexpected, unintended, and undesirable patient outcomes: death, physical/psychological injury, or any unexpected variation in a process or outcome that demands notice. Errors are deviations from accuracy or correctness, usually, caused by a fault (mistake) for example, carelessness, misjudgment, or forgetfulness. Most errors have no obvious effect on patients, yet most (82%) preventable complications in the past involved human error (Cooper et al., 1978). Errors are categorized according to persons or systems (Reason, 2000). Person approach refers to individual human error: forgetfulness, inattention, lapses (temporary failure of memory), preoccupation, violation (conscious deviation from a rule), loss of situational awareness, and fixation errors. Human errors lead to specific technical, judgmental, or monitoring mistakes, examples of which are given in Table 1.1. System approach refers to practice conditions: staff training, equipment, schedule density, health history gathering, policies, procedures, checklists, and so on. Latent errors can lay dormant in practices for years, only to be exposed during a triggering event, which then leads to an adverse outcome in a susceptible patient. Although it is tempting to blame a complication on a single human error (e.g., the practitioner gave the wrong drug and the patient died), seldom is this the case. Most complications are now known to be due to an unfortunate temporal alignment of a series of errors, which results in injury. These errors can arise from multiple