Miracle-dz/UniFAR-FTUs huggingface dataset:
A STUDY ON LOAD BALANCING AND ENERGY EFFICIENCY ALGORITHM FOR MANAGING THE RESOURCES ACROSS HOST MACHINE IN CLOUD

Cut-Binary Tree algorithm for VM Placement: Hierarchical clustering is achieved by using the minimum cuts in the undirected graph which we are passing as an input for the algorithm [5] where each vertex is a collection of VM’s and Path is referred as traffic between VM's. Traffic between the VM’s is assigned the Non-negative capacity. And the capacity of cut is determined by sum of all edges. Minimum Cut problem is mainly used to find the smallest capacity in a given undirected graph. And this undirected graph is expressed in terms of the binary tree where it contains Left and Right Sub-trees; Right sub-tree traffic is greater than left Subtree, Leaf nodes represent only one VM.
Effect of Calcium Soap Supplementation Without or With Rumen- Protected Methionine and Lysine in Lactating Buffaloes' Ration on Milk Production and Composition

This investigation was carried out to study the effect of calcium soap fatty acid as energy concentration in diets without or with rumen-protected amino acids (methionine and lysine) on lactating Egyptian buffaloes performance. Four lactating buffaloes were used in single 4x4 Latin Square experiment. All animals were subjected to four different experimental diets; 1) control diet, consisted of concentrate feed mixture, berseem clover and rice straw without supplementation, 2) control diet supplemented with 400 g protected fat source (calcium soap, Magnapac®); 3) control diet supplemented with rumen-protected amino acids (15g Methionine + 40g Lysine); and 4) control diet supplemented with (400g Magnapac® + 15g Methionine + 40g Lysine). Results obtained indicated that actual milk yield did not increase, but the amount of 7% fat corrected milk (FCM) increased significantly (p 0.05).
Strategies for improving ruminant utilisation of highgrain diets: Pangenome of Streptococcus bovis

The field of microbiology is being transformed by the increased ease and reduced costs of DNA sequencing. An initiative of the United States Department of Energy (USDOE), Joint Genome Institute facilitated the establishment of a Community Sequencing Project, the Hungate 1000, an international collaborative venture which aimed to sequence the genomes of bacteria isolated from the rumen and intestinal tract of herbivores. The DAF Rumen Ecology Unit (REU) contributed 36 rumen bacteria and seven bacteriophages to this genome sequencing effort. Thirteen of these bacteria belonged to the Streptococcus bovis/Streptococcus equinus complex (SBSEC). These are virulent, fast-growing commensal bacteria which over-proliferate in the rumen of cattle when they transition too quickly from forage to high grain feedlot rations, causing rumen lactic acidosis. Costly management strategies based on lengthy feed transition times (up to 20 days), and the use of feed additives including rumen modifiers such as monensin and antibiotics, are currently used to control rumen acidosis. This project used the SBEC genome sequences generated by the Hungate 1000, as well as publically available SBEC genome sequences, to create a combined genome dataset or pangenome. This pangenome of 42 genome sequences was then used to identify: 1. factors which enable these organisms to rapidly proliferate on starch-rich diets, for example, carbohydrate degrading enzymes; and 2. new approaches to control these organisms, for example inhibitory peptides (bacteriocins) and enzymes, carried by naturally-occurring viruses predatory to bacteria (known as bacteriophage or phage), which can specifically target and puncture bacterial cell walls. During the course of the project, technical capacity of the REU was also developed to facilitate the analysis and secure storage of large sequence datasets, with the expansion of high performance computing resources and installation of specialist software. Several of the phage proteins identified by the pangenome analysis were shown to be intact and functional, with the formation and release of phage particles from bacterial host cells verified by transmission electron microscopy and proteomics analysis. These phage proteins represent potential candidates for further investigation as antimicrobials to control SBEC, and will be used as background proof of concept for the development of project proposals for submission to funding agencies.
Rheology and conductivity of phloem

by Sierra Dawn Beecher, Ph.D. Washington State University July 2016 Chair: Michael Knoblauch The Pressure Flow Hypothesis, presented in 1930 by Ernst Münch, is the most accepted model for photoassimilate translocation by the phloem of plants. Net photosynthetic sugar production occurs in source tissues, often mature leaves. These photoassimilates are transported along the sieve tube system to consumptive sink tissues such as roots, meristems, and seeds. Münch’s hypothesis states that a sugar concentration gradient between sources and sinks osmotically generates a hydrostatic pressure differential that drives sap flow. If phloem sap is driven by a pressure differential, geometries of the sieve tube system, and the rheology of phloem sap should scale to equations describing pressure flow in low Reynolds number tubes. Imaging methods developed in this work provide robust geometric and rheological data, expanding the phloem biophysics toolkit. Additionally, insight into a mysterious phenomenon in which transient inhibition of translocation is induced by sudden localized stem chilling is presented. A novel application of fluorescence recovery after photobleaching (FRAP) allowed simultaneous measurement of sap velocity, and diffusivity of a phloem-mobile probe. This technique was used to investigate the cold shock phenomenon directly at the site of cold stimulus at higher resolution than was previously possible. Cold-induced sap velocity declines were not accompanied by similar reductions in sap viscosity. A Fluorescence Lifetime Imaging Microscopy
Nanotechnological approaches for counteracting multidrug resistance in cancer

Every year, cancer accounts for a vast portion of deaths worldwide. Established clinical protocols are based on chemotherapy, which, however, is not tumor-selective and produces a series of unbearable side effects in healthy tissues. As a consequence, multidrug resistance (MDR) can arise causing metastatic progression and disease relapse. Combination therapy has demonstrated limited responses in the treatment of MDR, mainly due to the different pharmacokinetic properties of administered drugs and to tumor heterogeneity, challenges that still need to be solved in a significant percentage of cancer patients. In this perspective, we briefly discuss the most relevant MDR mechanisms leading to therapy failure and we report the most advanced strategies adopted in the nanomedicine field for the design and evaluation of ad hoc nanocarriers. We present some emerging classes of nanocarriers developed to reverse MDR and discuss recent progress evidencing their limits and promises.
Proportional analysis of non-proportional loadings

Low-cycle-fatigue damage characterization requires inclusion of both elastic and plastic components of cyclic hysteresis. Incremental plasticity needs to be used for understanding and estimating plastic strain on the plane of crack initiation or evaluation of the plastic energy. Total deformation plasticity, despite its obvious shortcomings, has some researchers’ attention as an alternative tool for plastic analysis required for damage characterization. As long as the loading path remains proportional results are reliable. However, for out-of-phase nonproportional loadings, some modifications of theories are needed. In the present work, a new total-deformation formulation, capable of treating nonproportional loadings, is proposed. With this formulation, nonproportional loadings are treated in a proportional manner. Hence, computational time is reduced appreciably.
Study of Aspergillus fumigatus pathogenicity and identification of putative virulence genes

Aspergillus fumigatus is considered to be the most prevalent airborne pathogenic fungus. lts infection begins with conidial germination in lungs where different types of aspergillosis can be developed. In profoundly immunocompromised individuals this pathogen can even disseminate via the bloodstream, resulting in a disseminated disease in which other organs might be infected. With the airo of improving our understanding of the pathogenicity mechanisms, a new expression microarray covering the entire genome of A. fumigatus was designed to analyze its transcriptome at the first steps of germination and along a disseminated infection. According to transcriptomic results, essential elements such as nitrogen, iron and zinc seemed to be available enough during the infection, raising doubts about the potential of metal uptake systems as therapeutic or diagnostic methods. However, genes involved in carbohydrate and secondary metabolism, and even those encoding unclassified and hypothetical proteins, stood out as indispensable pathways for extending the infection. This study also allowed the identification of genes whose expressions were enhanced along the infectious process and the implication in virulence of two of them was studied. The deletion of the abrl/ brownl gene developed an isolate with an increase in cell damage, suggesting that this knockout might produce and secrete a toxic intermediate. On the contrary, when a transcription factor was deleted a decrease in cell damage was observed, highlighting its implication in A. fumigatus pathogenecity. Nevertheless, more studies need to be done to assess whether any of them show attenuation in virulence and to shed light on the biological functions they are involved, which could lead to identify potentially targets to develop diagnostic or treatment strategies.
Drug resistance in an age-of-infection model

ABSTRACT In the case of a disease spreading over a time-scale comparable to the average lifetime in a host population, when the infectiousness of individuals depends on the tine since the onset of infection and when infections involve both drug-sensitive and drug-resistant strains of a pathogen, resistance may develop during the treatment of drug-sensitive strains. If increasing the treatment rate reduces the reproduction number of the drug-sensitive strain to a value below the reproduction number of the drug-resistant strain, then the disease may persist at a boundary equilibrium where only drug-resistant infection is present.
Isolation and Characterization of D-Myo-Inositol-3-Phosphate Synthase Gene Family Members in Soybean

The objective of this research was to isolate genes encoding isoforms of the enzyme D-myo-inositol 3-phosphate synthase (MIPS, E.C. 5.5.1.4) from soybean and to characterize their expression, especially with respect to their involvement in phytic acid biosynthesis. A MIPS-homologous cDNA, designated GmMIPS1, was isolated via PCR using total RNA from developing seeds. Southern blot analysis and examination of MIPS-homologous soybean EST sequences suggested that GmMIPS1 is part of a multigene family of at least four similar members. The sequences of promoter and genomic regions of GmMIPS1 and GmMIPS2 revealed a high degree of sequence conservation. Northern and western blot analyses showed that MIPS transcript and protein are abundantly expressed early in seed development. Immunolocalization of MIPS protein in developing seeds confirmed expression of MIPS early in seed
Geographic patterns of alpine flora in China in relation to environmental and spatial factors

Alpine areas are global biomes with extreme environments and distinct boundaries. Studies on the geography of alpine flora help us to understand the formation of flora in an extremely cold and segregated environment, as well as the floristic relations between mountains. We integrated alpine flora data from 14 main mountain ranges in China, measured floristic similarities using the Jaccard index, and explored the percentage composition of areal-types of seed plants and the effects of geographical distance and environmental variation on flora similarities using correlation analyses and Mantel tests. Results indicated that Chinese al·研究报告·
The plant pathogen Erwinia amylovora: a proteome investigation

Partial funding by project no 101513 of the Agency of Innovation by Science and Technology (IWT‐Flanders, Belgium) is acknowledged. Michelle Holtappels is indebted to the IWT for a predoctoral fellowship. We thank Erik Royackers for technical assistance. We acknowledge the financial support from the Hercules Foundation in the framework of the project R‐3986 ‘LC‐MS@UHasselt: Linear Trap Quadrupool‐Orbitrap mass spectrometer’.
Identification of early biomarkers of neoplastic transformation in mouse tumour models of breast carcinogenesis

Tumour development is not an entirely cell-autonomous process, being dependent on the ability of bystander cells, mostly of bone marrow (BM) origin, to establish a pro-tumourigenic microenvironment. We hypothesize that identification of early BM changes would confirm that BM senses the onset of a neoplastic lesion in peripheral tissues even at very early stages of carcinogenesis and that such peripheral alterations could offer the opportunity to identify key signalling molecules that may represent early biomarkers of cancerogenesis and could allow discovering novel potential therapeutic targets. To test such hypothesis we took advantage of the MMTV-NeuT (NeuT) transgenic mouse model of mammary carcinogenesis and found that profound BM modifications in the composition and spatial arrangement of the hematopoietic populations, such as increase of immature myeloid granulocytic cells, contraction of B lymphoid pools, displacement of BM niches characterize NeuT mice with overt carcinomas in comparison to wild-type mice and that such modifications were already detectable at earlier stages of carcinogenesis, although less significant. Genes differentially expressed in the BM between transgenic and controls with overt tumour have been used to design an ad hoc “late gene signature” that has been tested and validated in two additional breast cancer models. Early NeuT BM samples already show differentially expressed genes in comparison to controls and they are efficiently separated by the late gene signature in two clusters: those with normal/mild dysplasia in their mammary glands from those with sever dysplasia/early carcinomas. Different circulating microRNA profiles characterized early stages of tumourigenesis and BM down-regulated genes are among their predictive targets. With an unprecedented level of integration between BM mRNA and microRNA profiles, BM immunolocalization, circulating microRNA profile and primary lesion histopathology we could demonstrate that the BM is an early sensor of incipient mammary tumour and that the alterations observed in advanced carcinomas are, at least in part, already present at early stages.
Pukia ohtsukai sp. nov., a new species of "cydippid" ctenophore from Japan

A new species of “cydippid” ctenophore, Pukia ohtsukai sp. nov., is described from material collected in Nishino-omote Port, Tanegashima Island, Japan. It can be distinguished from the only other member of the genus by the presence of perradial canals, lack of bilateral diverticula beneath the comb plates, a lower number of comb plates per comb row (19–21), the presence of black pigment patches at the oral ends of the comb rows and around the statocyst, and by the length of the pole plate, which extends only to the aboral tips of the comb rows.
Design and Evaluation of a Cross-cultural and Trans-disciplinary Global In- novation Course

From 2000 to 2005, he was with the Fab Operations Engineering Department, IBM Corporation, Burlington, VT, USA. He is currently an Associate Professor in the Department of Industrial and Systems Engineering at KAIST, South Korea. His research interests focus on semiconductor wafer manufacturing, persistent UAV service, education as a service and eco-design. He has published over 75 peer reviewed journal and conference papers in these areas.
EVIDENTIAL VALUE FROM INK-JET PRINTERS

The widespread use of ink-jet printers has made it necessary for document examiners to find a method of linking a questioned document to an individual ink-jet printer. The identification and characterisation of defects produced by ink-jet cartridges provides a means of achieving this. This study was primarily to investigate the physical nature of defects in inkjet printers and show how printer outputs and printers can be linked. Thus it was hoped to establish whether or not ink-jet printers can be linked to their print-outs through their physical appearance on the paper and if so to estimate the evidential value of any defects or other physical features. By identifying such features it was hoped that a protocol for the examination of this type of printer could be developed. Defects can be easily introduced into printouts by blocking the electrical defects that control a particular print-head. The behaviour of the defects thus produced is different for different types of ink-jet print cartridges. By studying the behaviour of such defects it is possible to devise a strategy for the examination to distinguish between different printers. Not all nozzles get blocked with the same frequency so if a particularly rare nozzle gets blocked then the evidential value in linking that particular printer to the printout will increase.
Pengaruh gaya keibubapaan terhadap stres akademik: kecerdasan emosi sebagai moderator

Kajian ini bertujuan untuk mengkaji pengaruh gaya keibubapaan (autoritarian, autoritatif dan pennisif) terhadap stres akademik dalam kalangan pelajar sekolah menengah. Selain itu, kajian ini turut mengkaji kecerdasan emosi sebagai moderator dalam hubungan antara gaya keibubapaan dengan stres akademik. Kajian ini berbentuk kuantitatif dengan menggunakan kaedah tinjauan melalui borang soal selidik dalam mengumpulkan data. Seramai 533 orang pelajar tingkatan empat dari enam buah sekolah sekitar daerah Kota Kinabalu, Sabah telah terpilih menjadi responden dalam kajian lni dengan menggunakan pensampelan rawak mudah. Satu set borang soal selidik telah digunakan, terdiri daripada Parental Authority Questionnaire (PAQ) untuk mengukur gaya keibubapaan, Academic Stress Scale (ASS) untuk mengukur stres akademik dan the Assessing Emotions Scale (AES) untuk mengukur kecerdasan emosi dalam kalangan pelajar. Data yang diperoleh dianalisis mengikut susunan hipotesis dengan menggunakan dua jenls ujlan laitu ujian regresl pelbagai metod stepwise dan ujian regresi hierarki dengan menggunakan perisian IBM Statistical Package for the Social Sciences (SPSS) versi 24. Hasil keputusan menunjukkan gaya keibubapaan autoritarian memberi pengaruh secara signifikan dan positif kepada stres akademik dalam kalangan pelajar dengan nilai sumbangan sebanyak 1.2 peratus varians. Meskipun nilai sumbangan varians adalah kecil, gaya keibubapaan autoritarian masih memberi kesan kepada stres akademik pelajar. Sebaliknya, gaya keibubapaan autoritatif dan pennisif pula tidak memberi pengaruh secara signifikan kepada stres akademik pelajar. Manakala, hasil analisls ujian regresl hierarki pula menunjukkan bahawa kecerdasan emosi tidak bertindak sebagal moderator dalam ketiga-tiga gaya keibubapaan terhadap stres akademik dalam kalangan pelajar. Implikasinya, hasil dapatan kajian ini amat berguna khususnya kepada golongan ibu bapa dalam mengaplikasikan gaya keibubapaan yang sesual terutama apabila menyentuh berkaitan akademik anak-anak kerana pendekatan yang kurang sesuai akan memberi kesan jangka panjang kepada psikologi pelajar.
Construction of the Recombinant Lentiviral Vector Containing Human GH1 Gene and Its Expression in HEK293T Cells

Human growth hormone (hGH) is a protein with multiple roles in a range of biological functions such as protein, carbohydrates and lipid metabolisms as well as immunity, tissue development and overall growth. One of the major class of biopharmaceuticals in mammalian cells is the production of recombinant pharmaceutical proteins. In this study, we constructed a lentiviral vector carrying coding region of human GH1 (hGH) gene in order to production of recombinant hGH in mammalian cell line. hGH gene was amplified from a plasmid containing full-length hGH coding sequence and then cloned into the lentiviral vector pCDH-GFP. The HEK293T cells were transduced by the lentivirus particles as a targeted cell. hGH expression status in the recombinant cells were confirmed by RT-PCR. Additionally, western blotting analysis results showed that the recombinant cells maintained a stable hGH expression during five weeks of continuous culture. In conclusion, results of current study suggested that constructed lentiviral vector can potentially be used for a stable production of recombinant hGH protein in HEK293T cells. This methodology could be served as a foundation for further research and may open new insights toward therapeutic protein manufacturing.
Antagonistic activity of plant-associated microorganisms against Phytophthora infestans

Phytophthora infestans is a pathogen threatening potato and tomato tillag es. Environmentally safe biological methods are searche d for the prevention against this pathogen. Many biocontrolling agents occur in plant s or on their surfaces. Our studies were aimed at searching for effective antagonists a gainst P. infestans among the isolates of endophyte and epiphyte bacteria. 37 endophyte an d 26 epiphyte bacterial strains isolated from Equisetum arvense, Elymus repens and Chenopodium album L. were studied. The bacterial strains were screened for their antag o istic activity against Phytophthora infestans. The inhibitory effect of the bacteria was estimat ed based on the calculation of the per cent of relative growth. All tested isolates sh owed antagonistic properties against P. infestans. The strongest activity was observed for the B. subtilis strain. Plant species and the tested parts of the plants had an important influence on the antagonistic activity of bacterial strains isolated from these plants. The e ndophyte growth rate inhibition of P. infestans was higher than for the epiphyte strains and was o ver 40% for most isolates. Bacterial biocontrolling agents should be searched amo ng endophytes of the studied plants and not among bacteria colonizing their phyllospher e.
Polimorfismos en el gen de la proteína c reactiva como marcadores de riesgo cardiovascular en población chiapaneca

Los sindromes coronarios agudos (SCA), como el infarto agudo de miocardio y la angina inestable, a nivel mundial son los principales responsables de la mortalidad por cardiopatia isquemica, de manera directa o indirecta; originados por la formacion de un trombo oclusivo, cuya causa mas frecuente es la aparicion de una fisura en la capsula fibrosa de las lesiones ateroscleroticas. Se ha establecido una clara relacion entre la presencia de actividad inflamatoria en las placas y la aparicion de SCA.
Palliative care: A review

The last quarter of the 20 century created broad advances in hospital and palliative care. Palliative care is an approach that elevates the personal satisfaction of patients and their families by challenging the issue related to perilous ailment. The objective is to avoid or treat the side effects or symptoms, at the correct time as conceivable that could be normal considering the present situation. This article provides an insight in the importance of palliative care and how various symptoms can be managed to enhance the quality of life in patients suffering from various life threatening diseases or malignancies.