Miracle-dz/UniFAR-FTUs huggingface dataset:
Long non-coding RNA H19 promotes proliferation of Hodgkin's lymphoma via AKT pathway.

PURPOSE To explore whether lncRNA (Long non-coding RNA) H19 could promote the development of Hodgkin's lymphoma (HL) by regulating cell proliferation via AKT pathway. METHODS H19 expressions in 60 HL tissues, 40 RH (reactive hyperplasia of lymph nodes) tissues, L428, A20 and Ly1 cell lines were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). H19 siRNA and pcDNA-H19 were constructed. Cell viability after altering H19 expression was detected by EdU and cell counting kit-8 (CCK-8) assay. The mRNA level of AKT in HL tissues and RH tissues was detected by qRT-PCR. The relationship between AKT and H19 was further detected by Western blot. RESULTS H19 was overexpressed in HL tissues and cell lines compared with those of controls. HL patients with huge lump and in Ann Arbor stage III-IV presented higher expression of H19. Besides, H19 expression was negatively correlated to overall survival (OS) of HL patients. In vitro experiments suggested that H19 downregulation decreased proliferation and viability of HL cells. AKT expression was upregulated in HL tissues compared with RH tissues, and was positively regulated by H19. Western blot results also indicated that H19 overexpression upregulated protein expression of AKT in HL cells. CONCLUSIONS Overexpressed lncRNA H19 promotes HL development by stimulating proliferation of HL cells via AKT pathway.
The influence of propofol on middle cerebral artery flow velocity (V MCA ) in patients with unruptured intracranial aneurysms during induction of general anaesthesia

Background: The estimated prevalence of unruptured intracranial aneurysms is 3%. Standard monitoring does not enable one to assess the influence of anaesthetics on the factors determining intracranial homeostasis. Thanks to transcranial Doppler ultrasonography, middle cerebral artery flow velocity (V MCA), reflecting cerebral blood flow, can be measured. The aim of the study was to assess the effects of propofol on intracranial homeostasis in patients with unruptured intracranial aneurysms during the induction of anaesthesia based on V MCA changes. Methods: The study encompassed 21 patients (group II) anaesthetised for elective craniotomy due to unruptured intracranial aneurysms. The control group (group I) included 21 patients who underwent discoidectomy. V MCA , as well as HR, MAP, etCO 2 , and SpO 2 were monitored at the following time points: T 0 — onset of study; T 1 — after 1 minute; T 2 — onset of preoxygenation; T 3 — after 1 minute of preoxygenation; T 4 — administration of fentanyl; T 5 — 1 minute after fentanyl; T 6 — administration of propofol; T 7 — 1 minute after propofol; T 8 — intubation; T 9 — 1 minute after intubation; T 10 — 2 minutes after intubation. Results: In both groups, no changes in mean HR, etCO 2 and SpO 2 were observed at the successive time points of observation. In groups I and II, an MAP decrease between T 6 and T 7 and an MAP increase between T 7 and T 9 were noted. There were no intergroup differences in mean values of MAP at the times of observation. In both groups and bilaterally, a V MCA decrease was recorded between T 6 and T 7 and an increase between T 7 and T 8 . There were no intergroup differences in mean values of V MCA at the times of observation. In both groups, a weak correlation between V MCA and MAP changes was found bilaterally. Conclusions: Propofol depresses the cerebral circulation during the induction of anaesthesia. The presence of an unruptured aneurysm does not affect the reactivity of the cerebral vessels during the induction of anaesthesia with propofol.
Browning and Beiging of Adipose Tissue: Its Role in the Regulation of Energy Homeostasis and as a Potential Target for Alleviating Metabolic Diseases Intact innervation is essential for diet-induced recruitment of brown adipose tissue

Fischer AW, Schlein C, Cannon B, Heeren J, Nedergaard J. Intact innervation is essential for diet-induced recruitment of brown adipose tissue. Am J Physiol Endocrinol Metab 316: E487–E503, 2019. First published December 21, 2018; doi:10.1152/ajpendo.00443. 2018. — The possibility that recruitment and activation of brown adipose tissue (BAT) thermogenesis could be beneficial for curtailing obesity development in humans prompts a need for a better understanding of the control of these processes [that are often referred to collectively as diet-induced thermogenesis (DIT)]. Dietary conditions are associated with large changes in blood-borne factors that could be responsible for BAT recruitment, but BAT is also innervated by the sympathetic nervous system. To examine the significance of the innervation for DIT recruitment, we surgically denervated the largest BAT depot, i.e., the interscapular BAT depot in mice and exposed the mice at thermoneutrality to a high-fat diet versus a chow diet. Denervation led to an alteration in feeding pattern but did not lead to enhanced obesity, but obesity was achieved with a lower food intake, as denervation increased metabolic efficiency. Conclusively, denervation totally abolished the diet-induced increase in total UCP1 protein levels observed in the intact mice, whereas basal UCP1 expression was not dependent on innervation. The denervation of interscapular BAT did not discernably hyper-recruit other BAT depots, and no UCP1 protein could be detected in the principally browning-competent inguinal white adipose tissue depot under any of the examined conditions. We conclude that intact innervation is essential for diet-induced thermogenesis and that circulating factors cannot by themselves initiate recruitment of brown adipose tissue under obesogenic conditions. Therefore, the processes that link food intake and energy storage to activation of the nervous system are those of significance for the further understanding of diet-induced thermogenesis.
$CSIsnoop$ : Inferring Channel State Information in Multi-User MIMO WLANs

Channel state information (CSI) has been proposed to enhance physical layer security by functioning as a shared secret between a transmitter and a receiver, because it decorrelates over half wavelength distances and cannot be predicted based on locations of the transmitter and receiver in rich scattering environments. Consequently, CSI was employed to generate passwords, to authenticate the source of packets, and to inject artificial noise to thwart eavesdroppers. However, in this paper, we present CSIsnoop, and show that an attacker can infer CSI in a multi-user MIMO WLAN, even when both channel sounding sequences from the access point and CSI measurement feedback from the clients are encrypted during downlink (explicit) channel sounding, or when uplink (implicit) channel sounding is employed. The insights of CSIsnoop are that the CSI of clients can be computed based on transmit beamforming weights at the access point, and that the transmit beamforming weights can be estimated from downlink beamforming transmission. In other words, we reveal the fundamental conflict between using CSI to optimize PHY design by beamforming and ensuring the confidentiality of CSI. We implement CSIsnoop on a software defined radio and conduct experiments in various indoor environments. Our results show that on average CSIsnoop can infer CSI of the target client with an absolute normalized correlation of over 0.99, thereby urging reconsideration of the use of CSI as a tool to enhance physical layer security in multi-user MIMO WLANs.
Desmoglein 1 Regulates Invadopodia by Suppressing EGFR/Erk Signaling in an Erbin-Dependent Manner

Loss of the desmosomal cell–cell adhesion molecule, Desmoglein 1 (Dsg1), has been reported as an indicator of poor prognosis in head and neck squamous cell carcinomas (HNSCC) overexpressing epidermal growth factor receptor (EGFR). It has been well established that EGFR signaling promotes the formation of invadopodia, actin-based protrusions formed by cancer cells to facilitate invasion and metastasis, by activating pathways leading to actin polymerization and ultimately matrix degradation. We previously showed that Dsg1 downregulates EGFR/Erk signaling by interacting with the ErbB2-binding protein Erbin (ErbB2 Interacting Protein) to promote keratinocyte differentiation. Here, we provide evidence that restoring Dsg1 expression in cells derived from HNSCC suppresses invasion by decreasing the number of invadopodia and matrix degradation. Moreover, Dsg1 requires Erbin to downregulate EGFR/Erk signaling and to fully suppress invadopodia formation. Our findings indicate a novel role for Dsg1 in the regulation of invadopodia signaling and provide potential new targets for development of therapies to prevent invadopodia formation and therefore cancer invasion and metastasis. Implications: Our work exposes a new pathway by which a desmosomal cadherin called Dsg1, which is lost early in head and neck cancer progression, suppresses cancer cell invadopodia formation by scaffolding ErbB2 Interacting Protein and consequent attenuation of EGF/Erk signaling.
Konzeption und Realisierung einer mobilen Android Anwendung zur Depressionserkennung

Depressionen werden heute immer haufiger und sollen 2020 die zweithaufigste Volkskrankheit sein. Sie zeichnen sich z. B. durch Interessenlosigkeit und Traurigkeit aus, konnen das Leben der Betroffenen stark beeintrachtigen und im schlimmsten Fall sogar zum Suizid fuhren. Um Depressionen zu erkennen, konnen Smartphones genutzt werden. Diese werden immer leistungsfahiger und somit wird auch das mobile Datensammeln immer haufiger eingesetzt. Das Datensammeln kann mit Hilfe von Fragebogen erfolgen. Gerade durch Fortschritte im Bereich von mobilem Internet wird dies ebenfalls unterstutzt, denn so konnen Fragebogen jederzeit online von zu Hause oder unterwegs ausgefullt werden. Ebenfalls ist eine direkte Auswertung und das Anzeigen von Feedback fur den Nutzer leichter. Daraus ergibt sich die Problemstellung Depressionen effizient zu erkennen. Das Personal in Arztpraxen erkennt Depressionen oftmals nicht oder Menschen mit Depressionen bekommen keine Behandlung, da sie sich durch Vermeidungsverhalten nicht die Moglichkeiten eroffnen, die bereitstehen. Doch durch fruhzeitige Erkennung konnen Depressionen oftmals gut behandelt werden. Im Rahmen dieser Arbeit wird eine mobile Android Anwendung fur Smartphones und Tablets konzipiert und implementiert. Es soll ein Modell aus Rollen, Studien und Fragebogen umgesetzt werden. Als Rollen gibt es Patienten und Arzte, die jeweils in ihrem Bereich der App verschiedene Funktionalitaten bereitgestellt bekommen. Ein Patient kann an Studien teilnehmen und die Fragebogen dieser ausfullen und abgeben. Ein Arzt ist als Verantwortlicher einer Studie dazu in der Lage alle abgegebenen Fragebogen dieser Studie auswerten zu lassen und fur den entsprechenden Patienten freizuschalten. Die Anwendung soll durch das Ausfullen und direkte Auswerten von Fragebogen dabei helfen Depressionen fruhzeitig zu erkennen, zur Behandlung wurde sie aber nicht konzipiert.
Contribution of PET-CT to staging, gross tumour volume definition, planning and response assessment in IMRT for nasopharyngeal carcinoma

Abstract The effectiveness of PET-CT (positron emission tomography–computed tomography) was investigated for staging target delineation compared with CT-MR (computed tomography–magnetic resonance) and early response of intensity-modulated radiotherapy (IMRT). Gross tumour volume–clinical target volume (GTV-CTV) differences between PET-CT and CT-MR for 14 nasopharyngeal carcinoma (NPC) patients were compared. Evaluation of doses of organs at risk (OARs) was done by IMRT plans. Responses of IMRT were evaluated with both sets. PET-CT changed MR-based TNM (Tumour Lymph Nodes Metastasis) in 11 of 14 patients. The median GTVNP (nasopharyx gross tumour volume) was 49.25 and 18.8 cm3 for CT-MR and PET-CT, respectively. In eight cases, GTVNP in the PET-CT was smaller than the CT-MR. The PET-CT presented a larger GTVNP than the CT-MR for six cases. Mean doses for the parotid glands were found to be higher than in CT-MR-based plan in one patient although he had smaller GTVNP at the PET-CT. The median follow-up was 16 months. Only one patient experienced recurrence in the CTVNP (nasopharyx clinical target volume). MR showed a decrease in the size-number of lymph nodes in four patients whereas PET-CT showed no uptake. All patients had positive responses to IMRT in their second control MR and PET-CT. PET-CT could improve tumour delineation. This enables an increase in dose inside the CTV. PET-CT provided significant information on the control scans for most of our patients whose MR imaging showed residual or recurrence.
The why and how our trauma patients die: A prospective Multicenter Western Trauma Association study

BACKGROUND Historically, hemorrhage has been attributed as the leading cause (40%) of early death. However, a rigorous, real-time classification of the cause of death (COD) has not been performed. This study sought to prospectively adjudicate and classify COD to determine the epidemiology of trauma mortality. METHODS Eighteen trauma centers prospectively enrolled all adult trauma patients at the time of death during December 2015 to August 2017. Immediately following death, attending providers adjudicated the primary and contributing secondary COD using standardized definitions. Data were confirmed by autopsies, if performed. RESULTS One thousand five hundred thirty-six patients were enrolled with a median age of 55 years (interquartile range, 32–75 years), 74.5% were male. Penetrating mechanism (n = 412) patients were younger (32 vs. 64, p < 0.0001) and more likely to be male (86.7% vs. 69.9%, p < 0.0001). Falls were the most common mechanism of injury (26.6%), with gunshot wounds second (24.3%). The most common overall primary COD was traumatic brain injury (TBI) (45%), followed by exsanguination (23%). Traumatic brain injury was nonsurvivable in 82.2% of cases. Blunt patients were more likely to have TBI (47.8% vs. 37.4%, p < 0.0001) and penetrating patients exsanguination (51.7% vs. 12.5%, p < 0.0001) as the primary COD. Exsanguination was the predominant prehospital (44.7%) and early COD (39.1%) with TBI as the most common later. Penetrating mechanism patients died earlier with 80.1% on day 0 (vs. 38.5%, p < 0.0001). Most deaths were deemed disease-related (69.3%), rather than by limitation of further aggressive care (30.7%). Hemorrhage was a contributing cause to 38.8% of deaths that occurred due to withdrawal of care. CONCLUSION Exsanguination remains the predominant early primary COD with TBI accounting for most deaths at later time points. Timing and primary COD vary significantly by mechanism. Contemporaneous adjudication of COD is essential to elucidate the true understanding of patient outcome, center performance, and future research. LEVEL OF EVIDENCE Epidemiologic, level II.
From omics technologies to personalized transfusion medicine

ABSTRACT Introduction: Blood transfusion is the single most frequent in-hospital medical procedure, a life-saving intervention for millions of recipients worldwide every year. Storage in the blood bank is an enabling strategy for this critical procedure, as it logistically solves the issue of making ~110 million units available for transfusion every year. Unfortunately, storage in the blood bank promotes a series of biochemical and morphological changes to the red blood cell that compromise the integrity and functionality of the erythrocyte in vitro and in animal models, and could negatively impact transfusion outcomes in the recipient. Areas covered: While commenting on the clinical relevance of the storage lesion is beyond the scope of this manuscript, here we will review recent advancements in our understanding of the storage lesion as gleaned through omics technologies. We will focus on how the omics-scale appreciation of the biological variability at the donor and recipient level is impacting our understanding of red blood cell storage biology. Expert commentary: Omics technologies are paving the way for personalized transfusion medicine, a discipline that promises to revolutionize a critical field in medical practice. The era of recipient-tailored additives, processing, and storage strategies may not be too far distant in the future.
Obstetric outcome of teenage pregnancies in a tertiary care centre in Mangalore, Karnataka, India

Background: Teenage pregnancy is a social problem affecting maternal and child health. In India, teenage pregnancy is an important public-health problem, although the national policy of the Government of India advocates the minimum legal age of marriage for girls to be 18 years. The aim of the present study was to observe the obstetric outcome in Teenage pregnancies Methods: A retrospective analysis of all Teenage pregnancies was done over a period of 1 year from August 2014 to July 2015. Case sheets of all teenage mothers delivered during that period were retrieved. Maternal and neonatal outcome were analyzed and compared with pregnant women ≥20 years of age. Results: 5859 deliveries occurred during the study period, teenage deliveries were 457.The prevalence of teenage deliveries was 7.8%. Cesarean delivery rate was 56% vs 42% (p value 0.05). Teenage mothers had increased Low birth weight rates 24% vs 10% (p<0.05). Conclusions: Teenage pregnancies has a negative impact on maternal and perinatal health. Hence strict laws should be enforced to prohibit teenage marriages. Adolescent girls should be educated about safe sex practices, contraception and complications of teenage pregnancies on maternal and child health.
Association Between Diethylhexyl Phthalate Exposure and Thyroid Function: A Meta-Analysis.

BACKGROUND Diethylhexyl phthalate (DEHP) is widely used in industrial products, particularly as plasticizers and softeners. Because it is used extensively, DEHP has been detected in humans worldwide. Although epidemiological studies suggest that DEHP can disrupt the function of the hypothalamic-pituitary-thyroid (HPT) axis, evidence on the association between DEHP exposure and thyroid function remains inconclusive. Therefore, a comprehensive meta-analysis was performed to investigate the association between DEHP exposure and the HPT axis in humans. METHODS A literature search of the MEDLINE, EMBASE, and Web of Science databases was conducted to search for studies in which the correlation coefficient values or regression coefficient values between three major DEHP metabolites (i.e., monoethylhexyl phthalate [MEHP], mono [2-ethyl-5-hydroxyhexyl] phthalate [MEHHP], and mono [2-ethyl-5-oxohexyl] phthalate) and thyrotropin, free thyroxine (T4), or total T4 were determined. The association between DEHPs and thyroid hormone levels were evaluated using Pearson's correlation coefficients. RESULTS Thirteen eligible articles were included. Urinary MEHP and MEHHP concentration was negatively correlated with total T4. Pooled correlation coefficients between MEHP/MEHHP and total T4 were -0.02 [confidence interval (CI) -0.05 to 0.00] and -0.03 [CI -0.05 to -0.01], respectively. Urinary mono (2-ethyl-5-oxohexyl) phthalate concentration was positively correlated with thyrotropin, and the pooled correlation coefficient was 0.02 [CI 0.00-0.04]. CONCLUSIONS The findings of this meta-analysis suggest a significant association between the exposure of DEHP metabolites and the function of the HPT axis.
Constitutional mosaicism in RASA1‐related capillary malformation‐arteriovenous malformation

Capillary malformation‐arteriovenous malformation (CM‐AVM) is caused by germline RASA1 and EPHB4 alterations. RASA1 intralesional second hits have also been reported. Here we report RASA1 constitutional mosaicism, defined here as the presence of a mosaic variant in all cell types of an individual, in two patients with CM‐AVM. High‐throughput sequencing was used to search for RASA1 pathogenic variants in blood samples from two unrelated patients with CM‐AVM. An affected tissue sample from one of the patients was also analyzed. Both patients showed different nonsense RASA1 variants in mosaic, ranging from 7% to 21.5%, in blood samples and in the corresponding affected tissue sample from one of the patients. In conclusion, we report for the first time the presence of RASA1 constitutional mosaicism in CM‐AVM. Constitutional mosaicism has implications for accurate molecular diagnosis and recurrence risk and helps to explain the great phenotypic variability in CM‐AVM.
Heat-Stable Enterotoxins of Enterotoxigenic Escherichia coli and Their Impact on Host Immunity

Enterotoxigenic Escherichia coli (ETEC) are an important diarrhea-causing pathogen and are regarded as a global threat for humans and farm animals. ETEC possess several virulence factors to infect its host, including colonization factors and enterotoxins. Production of heat-stable enterotoxins (STs) by most ETEC plays an essential role in triggering diarrhea and ETEC pathogenesis. In this review, we summarize the heat-stable enterotoxins of ETEC strains from different species as well as the molecular mechanisms used by these heat-stable enterotoxins to trigger diarrhea. As recently described, intestinal epithelial cells are important modulators of the intestinal immune system. Thus, we also discuss the impact of the heat-stable enterotoxins on this role of the intestinal epithelium and how these enterotoxins might affect intestinal immune cells. Finally, the latest developments in vaccination strategies to protect against infections with ST secreting ETEC strains are discussed. This review might inform and guide future research on heat-stable enterotoxins to further unravel their molecular pathogenesis, as well as to accelerate vaccine design.