Miracle-dz/UniFAR-FTUs huggingface dataset:
Electrical Muscle Activity Pattern and Transcriptional and Posttranscriptional Mechanisms Regulate PKA Subunit Expression in Rat Skeletal Muscle

We have examined protein kinase A (PKA) subunit expression in adult rat skeletal muscles. Northern blots identified PKA catalytic alpha and regulatory (R) I alpha and RII alpha subunits as the major subunits expressed in slowly contracting soleus (SOL) and rapidly contracting extensor digitorum longus (EDL) muscles. In addition, the steady-state RNA levels of PKA subunit mRNAs and activities of RI alpha and RII alpha promoters are similar in SOL and EDL. These data indicate that posttranscriptional mechanisms account for the twofold differences in PKA subunit protein levels reported earlier. Electrical stimulation of denervated SOL with an EDL-like activity pattern (fast pattern) transformed SOL into an EDL-like muscle with regard to PKA protein levels. These experiments suggest that the posttranscriptional regulation is activity pattern-dependent. Denervation specifically increased RI alpha promoter activity and RI alpha mRNA levels in SOL and EDL. Further experiments indicated that the RI alpha 1a upstream sequences were activated following denervation. Direct electrical stimulation prevented the rise in RI alpha mRNA levels following denervation, demonstrating that electrical muscle activity regulates transcription.
Positive feedback regulation between Akt2 and MyoD during muscle differentiation. CLONING OF Akt2 PROMOTER.

Satoshi Kaneko, Richard I. Feldman, Lu Yu, Zhenguo Wu, Tatiana Gritsko, Sue A. Shelley, Santo V. Nicosia, Tsutomu Nobori, and Jin Q. Cheng This article has been withdrawn by the authors. The same data were used to represent different experimental conditions. Specifically, lanes 2 and 3 from the RNA panel from 10T1/2 were reused in lanes 1 and 2 from the RNA panel from MyoD-10T1/2 in Fig. 2C. The authors state that they stand by the overall conclusions of the study. THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 291, NO. 43, p. 22850, October 21, 2016 © 2016 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.
Progression of experimental chronic peri-implantitis in dogs: clinical and radiographic evaluation.

BACKGROUND The aim of this study was to evaluate the progression of experimental peri-implantitis in dogs using implants with different surface coatings. METHODS Thirty-six dental implants with four different surface coatings, commercially pure titanium (cpTi), titanium plasma sprayed (TPS), hydroxyapatite (HA), and acid-etched (AE), were placed in six mongrel dogs. Five months after implantation, peri-implantitis was induced by cotton ligatures to facilitate plaque accumulation for 60 days. After 60 days, the ligatures were removed and supragingival plaque control was initiated for 12 months. Probing depth (PD), clinical attachment level (CAL), vertical bone level (VBL), horizontal bone level (HBL), and mobility were obtained at baseline, and 20, 40, 60 (acute phase), and 425 days (chronic phase) after ligature removal. RESULTS PD and CAL changed around all implant surfaces after ligature placement (P<0.0001). However, the means of PD and CAL were not statistically significant among the different surfaces (P>0.05). The range of CAL variation, calculated between baseline and 60 days (acute phase) and between 60 and 425 days (chronic phase), decreased (P<0.05). Bone loss increased during the entire experiment (P<0.0001). The HA surface showed the greatest bone loss measurement (5.06+/- 0.38 mm) and the TPS showed the smallest bone loss (4.27+/- 0.62 mm). However, statistical significance was not assessed for different coatings (P>0.05). CONCLUSIONS The clinical data at the initial phase showed rapid and severe peri-implant tissue breakdown. However, removal of ligatures did not convert the acute destructive peri-implant phase to a non-aggressive lesion and the progression of peri-implantitis was observed at chronic phase. The experimental peri-implantitis in dogs may be a useful model to evaluate the progression of peri-implantitis.
2011 ASA/ACCF/AHA/AANN/AANS/ACR/ASNR/CNS/SAIP/SCAI/SIR/SNIS/SVM/SVS guideline on the management of patients with extracranial carotid and vertebral artery disease: executive summary

It is essential that the medical profession play a central role in critically evaluating the evidence related to drugs, devices and procedures for the detection, management or prevention of disease. Properly applied, rigorous, expert analysis of the available data documenting absolute and relative benefits and risks of these therapies and procedures can improve the effectiveness of care, optimize patient outcomes and favorably affect the cost of care by focusing resources on the most effective strategies. One important use of such data is the production of clinical practice guidelines that, in turn, can provide a foundation for a variety of other applications, such as performance measures, appropriate use criteria, clinical decision support tools and quality improvement tools. The American College of Cardiology Foundation (ACCF) and the American Heart Association (AHA) have jointly engaged in the production of guidelines in the area of cardiovascular disease since 1980. The ACCF/AHA Task Force on Practice Guidelines (Task Force) is charged with developing, updating and revising practice guidelines for cardiovascular diseases and procedures, and the Task Force directs and oversees this effort. Writing committees are charged with assessing the evidence as an independent group of authors to develop, update or revise recommendations for clinical practice. Experts in the subject under consideration have been selected from both organizations to examine subject specific data and write guidelines in partnership with representatives from other medical practitioner and specialty groups. Writing committees are specifically charged to perform a formal literature review; weigh the strength of evidence for or against particular tests, treatments, or procedures; and include estimates of expected health outcomes where data exist. Patient specific modifiers, comorbidities and issues of patient preference that may influence the choice of tests or therapies are considered. When available, information from studies on cost is considered but data on efficacy and clinical …
Landmine detection with holographic radar

Holographic radar is a promising method of detecting landmines. Taking advantage of high plan-view resolution, holographic radar is able to discriminate targets from clutter objects. However, as high-frequency electromagnetic waves attenuate fast in soil, the performance of holographic radar in field experiments needs to be validated. Hence, we conduct experiments in different environments with the holographic radar system developed by our lab. To speed up data acquisition, we adopt a radial sampling scheme. The corresponding data processing algorithm, named `wavenumber domain filtered backprojection', is presented and verified with numerical simulation and field experimental data. The experimental results confirmed the feasibility and efficiency of holographic radar in detecting shallowly buried targets.
Biosensing Based on Luminescent Semiconductor Quantum Dots and Rare Earth Up-Conversion Nanoparticles

A biosensor is a device incorporating a biological sensing element either intimately connected to or integrated within a transducer, which is mainly determined by specific molecular recognition such as enzyme-substrate, antibody-antigen and so on. Currently, with the development of nanoscience and nanotechnology, more and more interest has been focused on using nanoparticles to fabricate biosensors (Alivisatos, 2004; Katz, 2004; Rosi 2005). Several kinds of biological sensors based on semiconductor quantum dots (QDs), gold nanoparticles (GNPs), carbon nanotubes (CNTs), fullerene, dendrimer nanoparticles have been presented. Semiconductor QDs are a new class of fluorescent materials for biosensor. In comparison with conventional organic dyes and fluorescent proteins, they have unique optoelectronic properties with size-tunable light emission, superior signal brightness, resistance to photobleaching and broad absorption spectra for simultaneous excitation of multiple fluorescence colors (Alivisatos, 1996). However, the use of semiconductor QDs for biosensor application still has some limitations (Jaiswal, 2004), for instance, the potential toxicity of QDs may pose risks to human health and the environment under certain conditions (Derfus, 2004). In addition, the absorption of UV and visible light by biological samples often induces autofluorescence, which interferes with fluorescent signals obtained from exogenous biomarkers. Moreover, if biological samples are prolonged exposure to UV radiation, it would cause the samples photo-damage and mutation. The drawbacks of QDs in biosensing application have prompted the development of upconverting nanoparticles (UCNs) emerged as another class of new biosensing materials. Usually, UCNs exhibit intense visible or near-infrared light excited by near-infrared light according to the anti-stokes law. The UCNs also show a sharp emission bandwidth, long lifetime, tunable emission, high photostability, low bio-toxicity and good biocompatibility, which are less harmful to biological samples and have greater penetration depth through biological samples than conventional ultraviolet excitation. Moreover, UCNs can be easily coupled to proteins or other biological macromolecular systems and used in a variety of
Efficient encoding and decoding algorithm used in Reed-Solomon codes for multiple fault-tolerance memories

With the increasing density of transistor and the probability of MBUs (Multiple Bits Upsets), Hamming code is not enough to provide required reliability in memories. Therefore multiple error correction codes are necessity. In this paper, Reed-Solomon (RS) codes are proposed to protect memories against MBUs which can achieve higher error correction capability (e.g. 8 bits). Besides a novel and low overhead multiplication algorithm is proposed and used in RS codes encoding and decoding process. At last, the encoder and decoder are implemented using Verilog HDL and validated through a number of simulations. The experiment results show that compared with other ECCs, RS code has higher error correction capability and lower area overhead.
Airway Smooth Muscle: Is There a Phenotype Associated with Asthma?

Increases in airway smooth muscle (ASM) mass characterize the pathology of patients who died of asthma. Recent studies also show that bronchial biopsies from individuals diagnosed with mild-to-moderate asthma also have increased ASM mass. As a consequence of such increases and the role of ASM in regulating bronchomotor tone, ASM plays a pivotal role in asthma pathophysiology. In the following review, we summarize the clinical and basic science evidence that suggests that ASM is a phenotypically distinct tissue whose therapeutic manipulation is critical for overall asthma management.
Improved prognosis of patients with primary biliary cirrhosis that have a biochemical response to ursodeoxycholic acid.

BACKGROUND & AIMS Ursodeoxycholic acid (UDCA) improves laboratory liver test results in patients with primary biliary cirrhosis (PBC). Few studies have assessed the prognostic significance of biochemical data collected following UDCA treatment. We performed a prospective multicenter study of patients with PBC treated with UDCA to compare prognosis with biochemical response. METHODS PBC was classified as early (pretreatment bilirubin and albumin levels normal), moderately advanced (one level abnormal), or advanced (both levels abnormal). Biochemical response was defined as proposed by Pares (decrease in alkaline phosphatase [ALP] level>40% of baseline level or normal level), Corpechot (ALP level<3-fold the upper limit of normal [ULN], aspartate aminotransferase level<2-fold the ULN, bilirubin level<1-fold the ULN), and our group (Rotterdam; normalization of abnormal bilirubin and/or albumin levels). RESULTS The study included 375 patients, and median follow-up time was 9.7 (range, 1.0-17.3) years. The prognosis for early PBC was comparable with that of the Dutch population and better than predicted by the Mayo risk score. Survival of responders was better than that of nonresponders, according to Corpechot and Rotterdam criteria (P<.001). Prognosis of early PBC was comparable for responders and nonresponders; prognosis of responders was significantly better in those with (moderately) advanced disease. CONCLUSIONS Prognosis for UDCA-treated patients with early PBC is comparable to that of the general population. Survival of those with advanced PBC with biochemical response to UDCA is significantly better than for nonresponders. Thus, UDCA may be of benefit irrespective of the stage of disease. Prognostic information, based on bilirubin and albumin levels, is superior to that provided by ALP levels.
Heterologous repressor-operator recognition among four classes of tetracycline resistance determinants

Homologous and heterologous repressor-operator interactions among four different classes of tetracycline resistance determinants have been compared. These are represented by RP1/Tn1721 (class A), R222/Tn10 (class B), pSC101/pBR322 (class C), and RA1 (class D). By the use of the purified repressor proteins of class A (TetRA) and class B (TetRB), operator sequences of all four classes are recognized by both with an identical stoichiometry of four repressor subunits per control sequence, but with different affinities. In vitro transcription has been used to demonstrate regulatory activities of TetRA and TetRB upon all four classes of tet genes. Tetracycline acted as an inducer. A functional relationship among the tet regulatory systems was also shown in vivo by complementation of a class A tetR'-galK fusion mutant with the tetR genes of classes A, B, and C. Repression of tetRA-linked galactokinase was ca. 80% in the presence of tetRA or tetRC, and ca. 50% in the presence of tetRB. Taken together, these results demonstrate heterologous repressor-operator interaction, suggesting close relationships among the four classes of Tcr determinants.
Acceptability ratings of tracheoesophageal speech

Thirty‐one naive listeners rated the intelligibility and acceptability of tracheoesophageal, esophageal, and normal laryngeal speech of 15 adult speakers. Intelligibility was measured using a multiple‐choice word test while acceptability was rated on a seven‐point equal‐appearing interval scale. Judgments were made on the features of fluency, pitch/quality, speech rate, inflection, and overall acceptability. Tracheoesophageal speech was found not to differ significantly from normal speech in intelligibility, rate, and inflection, but was less acceptable for fluency, pitch/quality and overall acceptability. Tracheoesophageal speech was more acceptable than esophageal speech for all features measured.
Polymorphisms in Nucleotide Excision Repair Genes and DNA Repair Capacity Phenotype in Sisters Discordant for Breast Cancer

Interindividual differences in DNA repair capacity (DRC) may play a critical role in breast cancer risk. Previously, we determined that DRC measured via removal of in vitro–induced benzo[a]pyrene diolepoxide-DNA adducts in lymphoblastoid cell lines was lower in cases compared with controls among sisters discordant for breast cancer from the Metropolitan New York Registry of Breast Cancer Families. We have now determined genotypes for seven single nucleotide polymorphisms in five nucleotide excision repair genes, including Xeroderma pigmentosum complementation group A (XPA +62T>C), group C (XPC Lys939Gln and Ala499Val), group D (XPD Asp312Asn and Lys751Gln), and group G (XPG His1104Asp) and ERCC1 (8092 C>A) in a total of 160 sister pairs for whom DRC phenotype data were available. Overall, there were no statistically significant differences in average DRC for most of the genotypes. A final multivariate conditional logistic model, including three single nucleotide polymorphisms (XPA +62T>C, XPC Ala499Val, and XPG His1104Asp) and smoking status, only modestly predicted DRC after adjusting for case-control status and age of blood donation. The overall predictive accuracy was 61% in the model with a sensitivity of 78% and specificity of 39%. These findings suggest that those polymorphisms we have investigated to date in nucleotide excision repair pathway genes explain only a small amount of the variability in DRC. (Cancer Epidemiol Biomarkers Prev 2006;15(9):1614–20)
Reputation-Based Trust in federated testbeds utilizing user experience

In this paper a novel reputation-based trust framework in federated testbeds is presented, termed as Federated Trust and User Experience (FTUE) Framework. Our aim is to assist peer experimenters in properly selecting resources from the federated community by constructing a quantitative view of the trustworthiness of each testbed. The FTUE framework combines the perceived user experience, as provided by user feedback, with the monitoring data retrieved for the respective experiments. Several situations that may arise in conducting and evaluating experiments were identified, properly formulated and simulated. The results presented demonstrate that the FTUE framework has the ability to dynamically adjust the reputation under challenging conditions, as well as being resilient to malicious attacks. Therefore, it could provide a paradigm for dynamic reputation building and trustworthiness among testbed services and users.
Occupational solvent exposure, genetic variation of DNA repair genes, and the risk of non-Hodgkin’s lymphoma

The main objective of this study was to test the hypothesis that genetic variations in DNA repair genes may modify the association between occupational exposure to solvents and the risk of non-Hodgkin’s lymphoma (NHL). A population-based case–control study was conducted on Connecticut women including 518 histologically confirmed incident NHL cases and 597 controls. Unconditional logistic regression models were used to estimate the odds ratios and effect modification from the 30 single nucleotide polymorphisms in 16 DNA repair genes of the association between solvent exposure and the risk of NHL overall and subtypes. Single nucleotide polymorphisms in MGMT (rs12917) and NBS1 (rs1805794) significantly modified the association between exposure to chlorinated solvents and the risk of NHL (Pfor interaction=0.0003 and 0.0048, respectively). After stratification by major NHL histological subtypes, MGMT (rs12917) modified the association between chlorinated solvents and the risk of diffuse large B-cell lymphoma (Pfor interaction=0.0027) and follicular lymphoma (Pfor interaction=0.0024). A significant interaction was also observed between occupational exposure to benzene and BRCA2 (rs144848) for NHL overall (Pfor interaction=0.0042). Our study results suggest that genetic variations in DNA repair genes modify the association between occupational exposure to solvents and the risk of NHL.
Broadband Doherty power amplifiers: Advances and challenges

This paper expounds on the recent advancements in the area of broadband Doherty power amplifiers (DPA). It begins by outlining the major sources of performance limitations in DPAs when employed to efficiently amplify multi-band signals scattered over extended frequency bandwidth. It goes on to describe a number of novel techniques devised to broaden the bandwidth of classical two-way DPAs. Finally, a broadband three-way DPA is introduced as a potential solution to efficiently amplify signals with extended peak to average power ratios. Measurement results obtained using a number of broadband DPA demonstrators are reported.
p21waf1 mRNA Contains a Conserved Element in Its 3′-Untranslated Region That Is Bound by the Elav-like mRNA-stabilizing Proteins*

The Elav-like proteins are specific mRNA-binding proteins that regulate mRNA stability. The neuronal members of this family (HuD, HuC, and Hel-N1) are required for neuronal differentiation. In this report, using purified HuD protein we have localized a high affinity HuD binding site to a 42-nucleotide region within a U-rich tract in the 3′-untranslated region p21waf1 mRNA. The binding of HuD to this site is readily displaced by an RNA oligonucleotide encoding the HuD binding site of c-fos. The sequence of this binding site is well conserved in human, mouse, and rat p21waf1 mRNA. p21waf1 is an inhibitor of cyclin-dependent kinases and proliferating cell nuclear antigen and induces cell cycle arrest at G1/S, a requisite early step in cell differentiation. The identification of an Elav-like protein binding site in the 3′-untranslated region of p21waf1 provides a novel link between the induction of differentiation, mRNA stability, and the termination of the cell cycle.
Study on pricing level of C2C electronic commerce platform

In recent years, with the popularity of the Internet in China C2C shopping websites are booming. Although large-scale have accumulated the number of users, but still no one can achieve our profit C2C platform. As a new business model, electronic commerce has made a significant influence on people's life. Firstly this paper divides the user's utility into the utility associated with risk and the utility not related to risk. Because the particularity of the network environment, the user's utility is affected by risk factors. Secondly we focus on the factors influencing on optimal pricing level of C2C electronic commerce network in three stages, which included network externalities, income, the level of credit and service cost. Finally we draw some conclusions which provide certain guiding significance for people.
Management of suspected myocardial infarction before admission: updated audit

Although the timing of aspirin administration is not as critical as thrombolytic treatment in patients with suspected acute myocardial infarction, aspirin should be given as soon as possible after the onset of symptoms.1 2 We reported a low rate (3%) of aspirin administration by general practitioners in patients with suspected acute myocardial infarction.3 We also found a low rate of intravenous opiate administration and inappropriate use of intramuscular analgesia by general practitioners before patients were admitted to hospital.3 Most of the media's attention, however, focused on aspirin. We therefore repeated an audit of the rate of aspirin and opiate administration by general practitioners to patients with chest pain before their referral to this coronary care unit. We also assessed the final …
Numerical Analysis on Tunable Multilayer Nanoring Waveguide

We propose a multilayer nanoring waveguide with ultrahigh refractive indices in this letter. The field confinement of the multilayer nanoring waveguide is stronger than the same outside radius of graphene-coated nanowire waveguide from the comparison of the normalized mode area. The influence of changing structure parameters on the properties of nanoring waveguide is analyzed. Based on the characteristic of the tunable surface conductivity of graphene, the effective refractive indices and propagation lengths of graphene plasmons modes can be tuned by the chemical potential of graphene. Furthermore, the geometry of the multilayer nanoring waveguide is compact and the method of chemical vapor deposition can be used to manufacture the proposed waveguide.