Miracle-dz/UniFAR-FTUs huggingface dataset:
EST analysis of gene expression in early cleavage-stage sea urchin embryos.

A set of 956 expressed sequence tags derived from 7-hour (mid-cleavage) sea urchin embryos was analyzed to assess biosynthetic functions and to illuminate the structure of the message population at this stage. About a quarter of the expressed sequence tags represented repetitive sequence transcripts typical of early embryos, or ribosomal and mitochondrial RNAs, while a majority of the remainder contained significant open reading frames. A total of 232 sequences, including 153 different proteins, produced significant matches when compared against GenBank. The majority of these identified sequences represented 'housekeeping' proteins, i.e., cytoskeletal proteins, metabolic enzymes, transporters and proteins involved in cell division. The most interesting finds were components of signaling systems and transcription factors not previously reported in early sea urchin embryos, including components of Notch and TGF signal transduction pathways. As expected from earlier kinetic analyses of the embryo mRNA populations, no very prevalent protein-coding species were encountered; the most highly represented such sequences were cDNAs encoding cyclins A and B. The frequency of occurrence of all sequences within the database was used to construct a sequence prevalence distribution. The result, confirming earlier mRNA population analyses, indicated that the poly(A) RNA of the early embryo consists mainly of a very complex set of low-copy-number transcripts.
Building comprehensible customer churn prediction models: A multiple kernel support vector machines approach

The in-depth understanding of customers' behavior is a crucial means in CRM for identifying its driving force and developing effective and personalized service activities. Relatively little research notices that it is key important in the competitive market to build comprehensible customer churn prediction models which can provide enterprises explicit customer behavior patterns. In this paper, a multiple kernel support vector machines (MK-SVMs) based customer churn prediction model is proposed to encapsulate three knowledge discovery tasks, which are feature selection, class prediction and decision rule extraction, into a whole framework A two-stage iteration of two convex optimization problems is designed for simultaneously feature selection and class prediction. Based on the selected features, support vectors are used to extract decision rules. An open CRM dataset is used to evaluate the performance of this approach. Experimental results show that MK-SVMs achieve promising performance on the heavily skewed dataset by means of a re-balanced strategy, and the extracted rules achieves high coverage and low false-alarm with small number of preconditions.
Luneburg theory of visual geodesics in binocular space perception; an experimental investigation.

To the Editor: —Replying to Drs. Shipley and Walker, I wish briefly to say: Shipley and Walker claim that work done in the darkroom alleys cannot be said to have been carried out under scotopic conditions. Reply: Unquestionably, points of light in a darkroom are perceived by the foveal cones, but a few points of light in a wholly dark surround do not prevent scotopic adaptation (the stars are seen at night with dark-adapted eyes). Therefore, experiments with darkroom alleys involve foveal (cone) perception by eyes which are in a state of scotopic adaptation—a complex which has not been sufficiently examined by darkroom experimenters. However, I have no objection to using the term darkroom alleys and have in general used this term in my article. Shipley and Walker claim I conclude that the white lighted and darkroom alleys give the same results. Reply: Nowhere do I conclude that
Viruses of Fish: An Overview of Significant Pathogens

The growing global demand for seafood together with the limited capacity of the wild-capture sector to meet this demand has seen the aquaculture industry continue to grow around the world. A vast array of aquatic animal species is farmed in high density in freshwater, brackish and marine systems where they are exposed to new environments and potentially new diseases. On-farm stresses may compromise their ability to combat infection, and farming practices facilitate rapid transmission of disease. Viral pathogens, whether they have been established for decades or whether they are newly emerging as disease threats, are particularly challenging since there are few, if any, efficacious treatments, and the development of effective viral vaccines for delivery in aquatic systems remains elusive. Here, we review a few of the more significant viral pathogens of finfish, including aquabirnaviruses and infectious hematopoietic necrosis virus which have been known since the first half of the 20th century, and more recent viral pathogens, for example betanodaviruses, that have emerged as aquaculture has undergone a dramatic expansion in the past few decades.
Clathrin‐dependent trafficking of subtilase cytotoxin, a novel AB5 toxin that targets the endoplasmic reticulum chaperone BiP

Subtilase cytotoxin (SubAB) is the prototype of a new family of AB5 cytotoxins produced by Shiga toxigenic Escherichia coli. Its cytotoxic activity is due to its capacity to enter cells and specifically cleave the endoplasmic reticulum (ER) chaperone BiP. However, its trafficking within target cells has not been investigated previously. In Vero cells, fluorescence colocalization with subcellular markers established that SubAB is trafficked from the cell surface to the ER via a retrograde pathway similar, but not identical, to those of Shiga toxin (Stx) and cholera toxin (Ctx), with their pathways converging at the Golgi. The clathrin inhibitor phenylarsine oxide prevented SubAB entry and BiP cleavage in SubAB‐treated Vero, HeLa and N2A cells, while cholesterol depletion did not, demonstrating that, unlike either Stx or Ctx, SubAB internalization is exclusively clathrin‐dependent.
Problemas resueltos de teoría de sistemas

espanolLa obra contiene una coleccion de problemas resueltos de Teoria de Sistemas, que es una asignatura troncal del segundo curso de Ingenieria Industrial, cuyo contenido comprende los conceptos basicos como sistemas dinamicos continuos y discretos, modelizacion de sistemas fisicos, transformada de Laplace, transformada en Z, representacion interna, respuesta en frecuencia, etc. Los problemas estan clasificados en diferentes categorias y resueltos con explicaciones detalladas para ayudar al estudiante a comprender mejor los conceptos teoricos. catalaL'obra conte una col·leccio de problemes resolts de Teoria de Sistemes, que es una assignatura troncal del segon curs d'Enginyeria Industrial, el contingut de la qual abraca els conceptes basics com ara sistemes dinamics continus i discrets, modelitzacio de sistemes fisics, transformada de Laplace, transformada en Z, representacio interna, resposta en frequencia, etc. Els problemes estan classificats en diverses categories i estan resolts amb explicacions detallades per a ajudar l'estudiant a comprendre millor els conceptes teorics. EnglishThe book includes a collection of resolved problems of Systems Theory, which is a core subject in the second course of Industrial Engineering, whose content comprises the basic concepts such as discrete and continuous systems, modeling of physical systems, converted into Laplace, converted into Z, internal representation, frequency response, etc. The problems are classified in different categories and resolved with detailed explanations in order to provide the student with a better understanding the basic concepts.
Modeling of evolutionary game between SNS and user: From the perspective of privacy concerns

SNS (Social Networking Sites) privacy has become an increasingly serious problem. The previous researches on the relationship between privacy concerns and interests are mostly concentrated in the indifference nodes (user behavior only), but there are long-term social behaviors for people in the social network environment. From the perspective of the dynamic evolution, SNS and users are the two continuous game groups, most of the game players would like to take the long-term strategy in face of the interest. This paper innovatively studied the long-term evolutionary game based on privacy concerns and the interests of the two sides: SNS and users. We introduced privacy concerns as a factor into the game for the first time, and constructed the evolutionary game of the interests of both sides through building the payoff matrix.
The abnormal glandular smear: cytologic prediction, colposcopic correlation and clinical management

We reviewed recent cytological reporting of abnormal glandular cells on cervical smears in order to assess the predictive value of these reports and the contribution of colposcopy in the assessment of these abnormalities. The study consisted of a 5-year retrospective review of the clinical management of 80 women with abnormal glandular cells on a cervical smear, with clinical and histopathological data available for review in the interval 1992-1996. There were two groups of women: (i) those referred with gynaecological symptoms and (ii) those with screen detected abnormalities who were asymptomatic and significantly younger than the first group. The predictive value of a glandular smear for malignancy was 42.5% and for premalignancy 28.8%. The most common lesions detected were cervical intraepithelial neoplasia (CIN) (13), endometrial cancer (13), cervical adenocarcinoma (10) and cervical intraepithelial glandular neoplasia (CIGN) (8). Four cases of endometrial carcinoma presented through screening. In the remainder a variety of benign conditions were identified as responsible for the abnormal smear. Failure to find an explanation for the abnormal smear only occurred in 8.8%. In developing a protocol for abnormal glandular smears, our observations indicate that: (a) those with abnormal bleeding require endometrial sampling; (b) for those with screen detected abnormality, colposcopy is valuable as it is a sensitive predictor of early invasion and can predict glandular abnormality; (c)diseases of the entire genital tract, non-gynaecological viscera and metastatic cancer can generate cytological abnormality; (d) screen detected borderline abnormality in endocervical cells is associated with CIN III.
Treatment utilization and unmet treatment need among Hispanics following brief intervention.

BACKGROUND In a large randomized trial examining ethnic differences in response to a brief alcohol intervention following an alcohol-related injury, we showed that Hispanics, but not non-Hispanics, were more likely to reduce alcohol intake in comparison with treatment as usual (Addiction 105:62, 2010). The current study evaluates whether the observed improvements in drinking outcomes previously reported among Hispanics following brief intervention might be related to prior or subsequent treatment utilization. METHODS This study is a secondary analysis of data collected in a randomized clinical trial that evaluated ethnic differences in the effect of a brief motivational intervention (BMI) on alcohol use among medical inpatients admitted for alcohol-related injury. For this study, statistical analyses were carried out to compare alcohol use, alcohol problems, treatment utilization, and unmet treatment need between Hispanic (n = 537) and non-Hispanic White (n = 668) inpatients. In addition, we examined the relationship between prior treatment utilization and unmet treatment need and alcohol use outcomes following brief intervention and the impact of brief intervention on subsequent treatment utilization and unmet treatment need. RESULTS In comparison with non-Hispanic Whites, Hispanics at baseline reported heavier drinking, more alcohol problems, greater unmet treatment need, and lower rates of treatment utilization. Among Hispanics, multilevel analyses showed that prior treatment utilization or unmet treatment need did not moderate the effect of BMI on alcohol outcomes. Furthermore, BMI did not significantly impact subsequent treatment utilization or unmet treatment need among Hispanics. Finally, treatment utilization and unmet treatment need at 6 months were not significant mediators between BMI and alcohol use outcomes at follow-up. CONCLUSIONS The benefits of brief intervention among Hispanics do not appear to be better explained by subsequent engagement in mutual help groups or formal substance abuse treatment. Prior history of treatment, regardless of the severity of alcohol problems, does not appear to influence the impact of brief intervention on alcohol use among Hispanics. These findings support prior results reporting the benefits of brief intervention among Hispanics and demonstrate that these improvements are not related to prior or subsequent treatment utilization.
Hacking into the emotion-creativity link : two new approaches to interactive systems that influence the relationship between emotion and creativity

Emotions can influence creative thinking. The ability of people to have the emotions that augment creativity can therefore help them to achieve higher creative task performance. How to design interactive systems that can effectively make use of this potential is, however, still an unanswered question. To explore possible answers to this question we have developed two novel approaches to interactive systems that can be used to effectively hack into the emotion-creativity link. One approach we developed enables a system to hack into the function of motor expressions in emotion regulation, in order to regulate the emotions that happen spontaneously during a creative task. We demonstrate that embodied interactions designed based on motor expressions, while used to interact with a system, can influence an intended emotion, and thereby influence the relationship between emotion and creativity. The second approach that we developed enables a system to hack into the cognitive appraisal processes that help cause emotion during a creative task. We demonstrate that believable computer generated feedback about the originality of a user’s own ideas, can be manipulated to help cause an intended emotion, determine its intensity, and thereby also influence the relationship between emotion and creativity. The contribution of this thesis is the development of two novel approaches to interactive systems that aim to influence the emotion-creativity link and in particular the explication of the mechanisms underlying these approaches. The studies form a novel contribution to both interactive systems research and the creativity sciences.
ANTIHYPOXIC ACTION OF 5-PHENYLPROPYNYLIDENE RHODANINE DERIVATIVE IN HYPOXIA-INDUCED RAT

Hypoxia,which is inadequate of tissue oxygen supply. Investigation of Antihypoxic activity in two models of experimental hypoxia i.e. haemic hypoxia and circulatory hypoxia. In addition, several studies suggest that thiazolidine derivative improve of oxygen supply to tissues under condition of its deficiency and decrease of incompletely oxidizes product of metabolism. Highly resistant and low resistant rats divided by their individual resistance to hypoxia. Pathological process developing in animals placed under the vacuum bell jar was made with exposure time 60 min.Medicinal agents (5-phenylpropynylidene rhodanines) was introduced and after modeling pathology in dose 1/10 LD50.The control group of animals was subjected without introducing of medicinal agents. Content of nitrite-ion in serum and in homogenate was determined applying methods. Reduction of nitric oxide synthesis was observed in liver hypoxic hypoxia. This can be accompanied by increase the level of lipoperoxidation processes and by suppression of antioxidant activity and mitochondrial enzymes. Furthermore the study indicated that hypoxia is inadequate of tissue oxygen supply, there was number of drugs for treatment of hypoxia such as circulatory hypoxia and haemic hypoxia. Evaluating the mechanism of 5-phenylpropynylidene rhodanine derivatives should show some potential action of antihypoxic activity.
Periventricular heterotopia in fragile X syndrome

The authors describe two unrelated individuals with fragile X syndrome (FXS) due to marked expansion and instability of the CGG trinucleotide repeats within the fragile X mental retardation 1 gene (FMR1) and periventricular heterotopia (PH). This observation suggests that the FMR1 gene is involved in neuronal migration and that abnormal neuronal migration, even beyond the resolution of MRI, contributes to the neurologic phenotype of FXS.
The role of high-frequency hearing in age-related speech understanding deficits

An experiment was conducted to further explore the effect of ageing on speech understanding under degraded listening conditions. Two groups of subjects, aged 20-39 years and 50-75 years were tested. Measurements were made of hearing thresholds in each ear from 0.25-10 kHz, consonant discrimination in quiet and continuous speech spectrum noise (S/N = −10 dB) and monophonic and stereophonic frequency selectivity. Neither group would have been diagnosed as hearing-impaired. Nonetheless, the older group had significantly higher hearing thresholds, which increased systematically with frequency. Poorer consonant discrimination in noise was observed for the older group. This outcome was correlated with high-frequency thresholds, not with age. There was no between-group difference in stereophonic frequency selectivity, minimizing the possibility of age-related changes in central auditory processing. Monophonic frequency selectivity, an index of cochlear processing, was correlated with speech understanding. The results support the conclusion that observed age-related effects are secondary to cochlear dysfunction.
High-pressure processing of Turkish white cheese for microbial inactivation.

High-pressure processing (HPP) of Turkish white cheese and reduction of Listeria monocytogenes, total Enterobacteriaceae, total aerobic mesophilic bacteria, total molds and yeasts, total Lactococcus spp. , and total Lactobacillus spp. were investigated. Cheese samples were produced from raw milk and pasteurized milk and were inoculated with L. monocytogenes after brining. Both inoculated (ca. 10(7) to 10(8) CFU/g) and noninoculated samples were subjected to HPP in a high-pressure food processor at 50 to 600 MPa for 5 and 10 min at 25 degrees C. Reductions in L. monocytogenes, total aerobic mesophilic bacteria, Lactococcus spp. , and Lactobacillus spp. in both pasteurized- and raw-milk cheese samples and reductions in total molds and yeasts and total Enterobacteriaceae counts in raw-milk cheese samples increased with increased pressure (P < or = 0.05). The maximum reduction of the L. monocytogenes count, ca. 4.9 log CFU/g, was obtained at 600 MPa. Because of the highly inhibitory effect of pasteurization, the total molds and yeasts and total Enterobacteriaceae counts for the cheese samples produced from pasteurized milk were below the detection limit both before and after HPP. There was no significant difference in inactivation of L. monocytogenes, total aerobic mesophilic bacteria, Lactococcus spp. , and Lactobacillus spp. under the same treatment conditions for the raw milk and pasteurized milk cheeses and for 5- and 10-min treatment times (P > 0.05). No significant change was detected in pH or water activity of the samples before and after HPP. Our findings suggest that HPP can be used effectively to reduce the microbial load in Turkish white cheese.
Substrate Integrated Waveguide Filters: Design Techniques and Structure Innovations

Because of the inherent structural flexibility in coupling design and topological arrangement, substrate integrated waveguide (SIW) filter topologies enjoy better out-of-band frequency selectivity and/or in-band phase response with the allocation of finite transmission zeros (FTZs). In the first article in this series, basic design rules and fundamental electrical characteristics have been presented that indicate the superior performances of SIW structures and their filter applications. Advanced design techniques and innovative structure features have recently been reported in a large number of publications. They include cross couplings realized by physical and nonphysical paths and SIW filters with dual-mode or multimode techniques. Miniaturization-enabled techniques including low-temperature cofired ceramic (LTCC) technology have been developed and applied to the development of SIW filters to reduce the size for low-gigahertz applications using nontransverse electromagnetic (non-TEM) modes. Wideband SIW filters, multiband SIW filters, and reconfigurable SIW filters have also been reported by various research groups. This article reviews these advanced and innovative SIW filter technologies, and related examples are presented and discussed.
Bioactivity of freeze-dried platelet-rich plasma in an adsorbed form on a biodegradable polymer material

Owing to the necessity for the immediate preparation from patients’ blood, autologous platelet-rich plasma (PRP) limits its clinical applicability. To address this concern and respond to emergency care and other unpredictable uses, we have developed a freeze-dried PRP in an adsorbed form on a biodegradable polymer material (Polyglactin 910). On the polymer filaments of PRP mesh, which was prepared by coating the polymer mesh with human fresh PRP and subsequent freeze-drying, platelets were incorporated, and related growth factors were preserved at high levels. This new PRP mesh preparation significantly and reproducibly stimulated the proliferation of human periodontal ligament cells in vitro and neovascularization in a chorioallantoic membrane assay. A full-thickness skin defect model in a diabetic mouse demonstrated the PRP mesh, although prepared from human blood, substantially facilitated angiogenesis, granulation tissue formation, and re-epithelialization without inducing severe inflammation in vivo. These data demonstrate that our new PRP mesh preparation functions as a bioactive material to facilitate tissue repair/regeneration. Therefore, we suggest that this bioactive material, composed of allogeneic PRP, could be clinically used as a promising alternative in emergency care or at times when autologous PRP is not prepared immediately before application.
Changeover Time Reduction and ProductivityImprovement by Integrating Conventional SMEDMethod with Implementation of MES for BetterProduction Planning and Control

This paper addresses a setup time reduction through SMED with an integration of MES (planning system interface). Reduction of changeover time has always been critical in most of the manufacturing industries. Most of the changeover or setup time reduction are governed or associated with Shingo’s Single Minute Exchange of Die(SMED) method that suggest the conversion of internal setup operations to external operations. SMED has been initiated by most of the companies but failed to implement it. The basic reason behind this is the strict application of SMED method which does not follow the systematic approach to implement this conversion and also it is not the best method to reduce the setup time in all the circumstances and situations. In this paper SMED integration with MES (which deals with Planning System Interface) is presented that has been developed specifically for an automotive supplier. The validation of the proposed method was done through implementation on manufacturing company which manufactures radiator, compressor and HVAC. The methodology used is SMED method with MES which deals with strategic definition and preparatory activities. It also includes communication which should be two ways so as the MES can keep the planning system properly informed about activities such as labour data, inventory changes, work order progress, project targets and timescale definition, selection of the appropriate team and coordinator, allocation of specific roles and responsibilities to each team member, training of team and shop floor staff on the new methodology and changeover standards. By implementing the SMED with MES improvement programme, the company achieved much reduction on changeover time which led to the increase in high productivity.