Miracle-dz/UniFAR-FTUs huggingface dataset:
The Anabolic Response to Dietary Protein Is Not Limited by the Maximal Stimulation of Protein Synthesis in Healthy Older Adults: A Randomized Crossover Trial

We have recently demonstrated in young adults that an anabolic response with mixed meal protein intake above ~35 g/meal, previously recognized as an “optimal” protein dose, was further stimulated. However, it is unknown if this applies to older adults. We therefore examined anabolic response to a mixed meal containing either 35 g (MOD, moderate amount of protein) or 70 g (HIGH, high amount of protein) in a randomized cross-over metabolic study in older adults (n = 8). Primed continuous infusions of L-[2H5] phenylalanine and L-[2H2]tyrosine were performed to determine whole-body protein kinetics and muscle protein fractional synthesis rate (MPS) in basal fasted and fed states. Whole-body protein kinetics (NB, net protein balance; PS, protein synthesis; PB, protein breakdown) and MPS was expressed as changes from the baseline post-absorptive state. Consistent with our previous findings in young adults, both feedings resulted in a positive NB, with HIGH being more positive than MOD. Furthermore, NB (expressed as g protein∙240 min) increased linearly with an increasing amount of protein intake, expressed relative to lean body mass. The positive NB was achieved due mainly to the suppression of PB in both MOD and to a greater extent HIGH, while PS was only increased in HIGH. Consistent with the whole-body data, MPS was significantly higher in HIGH than MOD. Plasma concentrations of essential amino acids and insulin were greater in HIGH vs. MOD. We conclude that in the context of mixed meals, whole-body anabolic response linearly increases with increasing protein intake primarily through the suppression of PB, and MPS was further stimulated with protein intake above the previously considered “optimal” protein dose in older adults.
Melissopalynology and antioxidant properties used to differentiate Schefflera abyssinica and polyfloral honey

Honey can be categorized as monofloral and polyfloral honey. There is a strong interest in science and commerce, to further differentiate honey. In the present study, Schefflera abyssinica and polyfloral honey from Sheka Forest, Ethiopia was investigated. Botanical origin was determined based on Melissopalynology. Refractive index, moisture, sugars, ash, pH, free acidity, hydroxymethylfurfural, optical density, diastase activity, protein, and color were determined based on the standard method of the international honey commission (IHC) and AOAC. Antioxidant activity and Antioxidant content were determined using UV- visible spectroscopy. The level of pollen dominancy for monofloral honey (Schefflera abyssinica) ranged from 76.2 to 85.8%. The polyfloral honey stuffed with a variety of pollen grain ranged from 2.2% (Coffea arabica) to 23.2% (Schefflera abyssinica). Schefflera abyssinica honey contained more total phenolic compounds (75.08 ± 2.40 mg GAE/100g), and total flavonoids (42.03 ± 1.49 mg QE/100 g), as well as had stronger DPPH (44.43 ± 0.97%) and hydrogen peroxide (78.00 ± 4.82%) scavenging activity. The principal component analysis revealed that Schefflera abyssinica honey associated with the antioxidant properties of total phenolic, total flavonoids, DPPH, and H2O2. , which revealed that floral honey sources can essentially differentiated by antioxidant patterns. The higher electrical conductivity (0.42 ± 0.02 mS/cm), ash (0.41 ± 0.05 g/100g), pH (4.01 ± 0.08), optical density (0.26 ± 0.03) and diastase activity (5.21 ± 0.17 Schade units) were recorded in polyfloral honey. Schefflera abyssinica and polyfloral honey satisfy the requirement of national and international standards. The pollen analysis in combination with antioxidant properties distinguishes Schefflera abyssinica from polyfloral honeys.
Early spring SST distribution in the Baltic Sea: in search of the coldest water

We examine three hypotheses of formation of waters of the Cold Intermediate Layer (CIL) in the Baltic Sea: the coldest baltic waters are formed (1) at the beginning of spring warming in the Arkona and Bornholm basins, (2) in the centers of mesoscale vortexes (similar to those in the Black Sea), and (3) in the convergence zones of alongshore fronts while cooling over shelves (as in the Mediterranean Sea).
Type 2 Diabetes Alters Vascular Cannabinoid Receptor 1 Expression, Phosphorylation Status, and Vasorelaxation in Rat Aorta

Previous studies have suggested a role of the endocannabinoid system in metabolic diseases, such as diabetes. We investigated the effect of diabetes on cannabinoid receptor type 1 (CB1) expression and cannabinoid-induced vasorelaxation in rat aorta rings. Aortas from healthy rats and from rats with experimentally induced diabetes were used to compare the vasorelaxant effect of the cannabinoid agonist arachidonylcyclopropylamide (ACPA) and CB1 expression and localization. After 4–8 weeks of diabetes induction, CB1 receptor expression and CB1 phosphorylation were higher in aortic rings, in association with greater vasorelaxation induced by the CB1 agonist ACPA compared to healthy rats. The vasorelaxant effect observed in healthy rats is similar throughout the study. Further studies are needed to elucidate the implications of CB1 receptor overexpression in diabetes and its influence on the progression of the cardiovascular complications of this metabolic disease.
Isolation and Characterization of Porcine Astrovirus 5 from a Classical Swine Fever Virus-Infected Specimen

Porcine astroviruses are mainly associated with gastroenteritis and neurological diseases in pigs, and five genotypes have been identified (PAstV1-5). However, the clinical manifestations of genotypes other than PAstV1 have not yet been determined because of the failure of in vitro virus isolation. Here, we report a surprising isolation of a PAstV5 strain from a clinical classical swine fever virus (CSFV)-infected tissue sample, which can stably passage in PK-15 cells, and coinfection with CSFV significantly enhanced the replication of PAstV5, possibly through suppression of beta interferon production. Thus, the first isolated PAstV5 strain will be useful for investigating the biological and pathogenic properties of this virus, and the findings obtained in this study provide new insights into defining the interaction mechanism between CSFV and PAstV5. ABSTRACT Many new astroviruses have been identified in humans and other animals in recent years, but only a few have been successfully isolated for extensive biological study. Here, we report an unusual isolation of a porcine astrovirus 5 (PAstV5) strain from a clinical classical swine fever virus (CSFV)-infected tissue sample. Incubation of porcine PK-15 cells with an extract of the CSFV-positive tissue resulted in unexpected cytopathic effects (CPEs), and high-throughput viromic sequencing identified PAstV5 and porcine circovirus type 2 (PCV2) as well as CSFV in the culture. After clearance of CSFV and PCV2, a pure PAstV5 strain, named PAstV5-AH29-2014, was obtained. Analysis revealed virus of typical astroviral morphology with a genome of 6,448 nucleotides, sharing 84.3 to 88.9% nucleotide identity with previously published PAstV5 strains. A mechanistic study showed that CSFV coinfection was likely an important factor for successful isolation by significantly enhancing PAstV5 replication in PK-15 cells via suppression of a type I interferon response. Altogether, PAstV5-AH29-2014, as the first isolated PAstV5 strain, will provide critical material for the investigation of the biological and pathogenic properties of this virus as well as for future development of relevant biological and diagnostic reagents. IMPORTANCE Porcine astroviruses are mainly associated with gastroenteritis and neurological diseases in pigs, and five genotypes have been identified (PAstV1-5). However, the clinical manifestations of genotypes other than PAstV1 have not yet been determined because of the failure of in vitro virus isolation. Here, we report a surprising isolation of a PAstV5 strain from a clinical classical swine fever virus (CSFV)-infected tissue sample, which can stably passage in PK-15 cells, and coinfection with CSFV significantly enhanced the replication of PAstV5, possibly through suppression of beta interferon production. Thus, the first isolated PAstV5 strain will be useful for investigating the biological and pathogenic properties of this virus, and the findings obtained in this study provide new insights into defining the interaction mechanism between CSFV and PAstV5.
External validation and clinical usefulness of three commonly used cardiovascular risk prediction scores in an Emirati population: a retrospective longitudinal cohort study

Objectives Cardiovascular disease (CVD) risk prediction models are useful tools for identifying those at high risk of cardiovascular events in a population. No studies have evaluated the performance of such risk models in an Arab population. Therefore, in this study, the accuracy and clinical usefulness of two commonly used Framingham-based risk models and the 2013 Pooled Cohort Risk Equation (PCE) were assessed in a United Arab Emirates (UAE) national population. Design A 10-year retrospective cohort study. Setting Outpatient clinics at a tertiary care hospital, Al-Ain, UAE. Participants The study cohort included 1041 UAE nationals aged 30–79 who had no history of CVD at baseline. Patients were followed until 31 December 2019. Eligible patients were grouped into the PCE and the Framingham validation cohorts. Exposure The 10-year predicted risk for CVD for each patient was calculated using the 2008 Framingham risk model, the 2008 office-based Framingham risk model, and the 2013 PCE model. Primary outcome measure The discrimination, calibration and clinical usefulness of the three models for predicting 10-year cardiovascular risk were assessed. Results In women, the 2013 PCE model showed marginally better discrimination (C-statistic: 0.77) than the 2008 Framingham models (C-statistic: 0.74–0.75), whereas all three models showed moderate discrimination in men (C-statistic: 0.69‒0.70). All three models overestimated CVD risk in both men and women, with higher levels of predicted risk. The 2008 Framingham risk model (high-risk threshold of 20%) classified only 46% of women who subsequently developed incident CVD within 10 years as high risk. The 2013 PCE risk model (high-risk threshold of 7.5%) classified 74% of men who did not develop a cardiovascular event as high risk. Conclusions None of the three models is accurate for predicting cardiovascular risk in UAE nationals. The performance of the models could potentially be improved by recalibration.
‘Lab-on-a-chip’ for cell engineering: towards cellular models mimicking in vivo

79 One of the main scopes of modern cell engineering is development of cellular models that can replace animals in drug screening and toxicological tests, so called alternative methods. Construction of the alternative model is a very challenging task due to a richness of factors creating the in vivo environment. Th e monolayer cell culture — cultivation of adhesive cells on artifi cial surfaces such as glass or polymer — lack most of the in vivo-like interactions, but still is the only tool for the majority of applications. One of the most prospective approaches on mimicking in vivo environment is “Lab-on-a-chip” technology. Microfl uidic devices off er lots of advantages over traditional in vitro culture, e.g. much higher cell volume-to-extracellular fl uid volume ratio or possibility of regulation of hydrodynamic stress. Th is presentation aims to introduce latest advances of our team in microfl uidic cell culture devices. Our novel approach is to cultivate three dimensional multicellular aggregates (spheroids) in microenvironments arranged in a microfl uidic system. Th e geometry and materials of the system allow for cultivation, observation and analysis of multicellular spheroids. Th e results presented concern multicellular tumor spheroids (MCTS) rising from human cancer cells, which are considered to represent most of the conditionings of cancer tumor in vivo. Th e fully developed MCTS microdevice will be a reliable tool for anticancer drug screening, as the results most likely will be in a close accordance with the results obtained in vivo.
Une théorie de la fonctionnelle de la densité moléculaire pour la solvatation dans l'eau

La theorie de la fonctionnelle de la densite classique est utilisee pour etudier la solvatation de solutes quelconques dans le solvant eau. Une forme approchee de la fonctionnelle d’exces pour l’eau est proposee. Cette fonctionnelle necessite l’utilisation de fonctions de correlation du solvant pur. Celles-ci peuvent etre calculees par simulations numeriques, dynamique moleculaire ou Monte Carlo ou obtenues experimentalement. La minimisation de cette fonctionnelle donne acces a l’energie libre de solvatation ainsi qu’a la densite d’equilibre du solvant. Differentes corrections de cette fonctionnelle approchee sont proposees. Une correction permet de renforcer l’ordre tetraedrique du solvant eau autour des solutes charges, une autre permet de reproduire le comportement hydrophobe a longue distance de solutes apolaires. Pour realiser la minimisation numerique de la fonctionnelle, la theorie a ete implementee sur une double grille tridimensionnelle pour les coordonnees angulaires et spatiales, dans un code de minimisation fonctionnelle ecrit en Fortran moderne, mdft. Ce programme a ete utilise pour etudier la solvatation en milieu aqueux de petits solutes atomiques neutres et charges et de petites molecules polaires et apolaires ainsi que de solutes plus complexes, une argile hydrophobe et une petite proteine. Dans chacun des cas la theorie de la fonctionnelle de la densite classique permet d’obtenir des resultats similaires a ceux theoriquement exacts obtenus par dynamique moleculaire, avec des temps de calculs inferieurs d’au moins trois ordres de grandeurs.
Thermohaline Fingerprints of the Greenland-Scotland Ridge and Fram Strait Subsidence Histories

Changes in ocean gateway configuration are known to induce basin-scale rearrangements in ocean characteristics throughout the Cenozoic. However, there is large uncertainty in the relative timing of the subsidence histories of ocean gateways in the northern high latitudes. By using a fully coupled General Circulation Model we investigate the salinity and temperature changes in response to the subsidence of two key ocean gateways in the northern high latitudes during early to middle Miocene. Deepening of the Greenland-Scotland Ridge causes a salinity increase and warming in the Nordic Seas and the Arctic Ocean. While warming this realm, deep water formation takes place at lower temperatures due to a shift of the convection sites to north off Iceland. The associated deep ocean cooling and upwelling of deep waters to the Southern Ocean surface causes a cooling in the southern high latitudes. These characteristic impacts in response to the Greenland-Scotland Ridge deepening are independent of the Fram Strait state. Subsidence of the Fram Strait for a deep Greenland-Scotland Ridge causes less pronounced warming and salinity increase in the Nordic Seas. A stronger salinity increase is detected in the Arctic while temperatures remain unaltered, which further increases the density of the North Atlantic Deep Water. This causes an enhanced contribution of North Atlantic Deep Water to the abyssal ocean and on the expense of the colder southern source water component. These relative changes largely counteract each other and cause little warming in the upwelling regions of the Southern Ocean.
Aspegillus terreus: From Soil to Industry and Back

Aspergillus terreus is an important saprophytic filamentous fungus that can be found in soils. Like many other soil microorganisms, A. terreus demonstrates multiple functions and offers various important metabolites, which can be used in different fields of human activity. The first application of A. terreus on an industrial level is the production of itaconic acid, which is now considered as one of the most important bioproducts in the Green Chemistry field. The general schemes for itaconic acid production have been studied, but in this mini-review some lines of future research are presented based on analysis of the published results. A. terreus is also intensively studied for its biocontrol activity and plant growth-promoting effect. However, this microorganism is also known to infect important crops such as, amongst others, rice, wheat, potato, sugar cane, maize, and soybean. It was suggested, however, that the balance between positive vs. negative effects is dependent on the soil-plant-inoculant dose system. A. terreus has frequently been described as an important human pathogen. Therefore, its safety manipulation in biotechnological processes for the production of itaconic acid and some drugs and its use in soil-plant systems should be carefully assessed. Some suggestions in this direction are discussed, particularly concerning the uses in crop production.
Standard and advanced separation: PUREX processes for nuclear fuel reprocessing

Abstract: The PUREX process for separating uranium and plutonium from irradiated nuclear fuels has been extensively studied and successfully operated industrially over the preceding five plus decades. It is anticipated that PUREX will play an important role in upcoming and advanced future nuclear fuel cycles. The first objective of this chapter is to provide the background information required to status the present state of the art as currently practiced at the industrial scale. The second objective is to examine the modifications ready, or nearly so, for implementation into the next generation of PUREX reprocessing facilities, thereby further expanding the utility and operation of the process for use in nuclear fuel cycles of the future.
Sur la structure électronique de quelques borures d'éléments de transition

2014 There is a strong analogy between the magnetic properties (magnetizations, Curie points and susceptibilities) and the residual resistivities of borides of general formula M1-xM’xB and (M1-xM’x)2B, where M and M’ are metals of the first transition series, and those of the metallic alloys M1-xM’x. This shows that a partially filled d type narrow band is of peculiar importance in the chemical binding of these compounds. The residual resistivities of the mixed borides for which x « 1 are theoretically related to their magnetic behaviour through the occurence of virtual bound states. LE JOURNAL DE PHYSIQUE TOME 27, MAI-JUIN 1966,
A survey on cloud computing, security Challenges, architecture, applications & solutions

In this paper, we are going to emphasize on the advancement in computing technology cloud computing is an incredible innovation to perform complex processing. It decreases the need of costly equipment’s and dedicated storage. The connection between information and cloud computing is inspected along . The definition, attributes and grouping of huge information along with certain discourses on cloud computing are presented with focus on accessibility, information honesty, information quality, adaptability, security and administration. There is a rapid development in cloud computing and Internet of things regard the field of wireless communication. We present a survey on the security issues of both the technologies. We studied about the contributions of cloud computing to the Internet of things technology. It will illustrate the improvements on the internet of things on the basis of cloud. We will discuss the pros and cons of merging the technologies. The financial effects on innovation will be discussed, it will mainly emphasize on macroeconomics development. In this examination the results of rivalry and market structures will be brought to spotlight . Secondly the impact on industries and firms renting the storage from the providers will be discussed and how it effects the economics. Vehicular networking has become a significant area due to standardization, traffic management, road safety and infotainment. Vehicles carry more communication systems in sensing power. It can promote intelligent transportation system. Vehicular cloud computing remarkably effects traffic, the executives and street security in a split by utilizing the vehicular assets.
Rediscovery of Vampyressa thyone (Chiroptera, Phyllostomidae) in Honduras: updated distribution and notes on its conservation

In Central America, Vampyressa is represented by two species, Vampyressa elisabethae and V. thyone. The latter is the only representative of the genus in Honduras and the most recent record was in 2007. Six surveys were carried out in the Departments of Cortes, Atlantida and Gracias a Dios. Only seven individuals of V. thyone were recorded between March 2016 and September 2017. The low abundance of V. thyone supports the fact that it is an uncommon species in the country; however, these records update the distribution in northern Honduras. We recommend systematic bat assessments on the three protected areas in which we recorded V. thyone for a better comprehension of the local populations on this species in the country
Is Deteriorating Air Quality Impacting Stock Performance in India? Air Quality Index Predicts Stock Performance

Background/objectives: This study attempts to examine a systematic relationship between air quality index (AQI) and the performance of stock markets in India. India has ranked among the bottom five countries in environmental performance index (EPI) by World Economic Forum (WEF). Previous studies in this field conducted in other parts of the world indicate a direct relation between environmental performance and market performance; however, the case of India may prove to be different considering the Indian environmental policy, environmental accountability, population, and income and development aspects. Methods: We have employed linear regression technique to ascertain the impact of AQI on stock returns. AQI data from 13 stations across India are the dependent variables. These dependent variables are each regressed individually for each stock market index from Bombay Stock Exchange (BSE) equity index, Sensex. Therefore, there are 17 models where AQI from the chosen 13 stations has been regressed with each of the industry specific index. A descriptive analysis is also performed to know the yearly averages of the AQI across stations used in the study. Findings: Our results confirm that there exists a negative association between AQI and stock returns for top-performing growth stocks. The results also indicate that there is a positive association between AQI and capital intensive industry stocks, while a negative association was observed in the case of labor-intensive industries. The findings indicate that AQI is a predictor for stock returns in India in the short term. Application: Environmental influences on stock market performance are becoming more potent than ever before as the negative externalities of pollution impact the financial performance of corporations.
Thermal profile of broilers infected by Eimeria tenella

In study, infrared thermography (IRT) was assessed as a means of detecting the changes in body temperature in poultry coccidiosis, as well as determining IRT used in imaging and measuring the regional changes in skin temperature that occur in the poultry during this infection. Broilers were inoculated with 5×10 2 or 1×10 3 oocysts of Eimeria tennella.The group of control animals remained non-inoculated throughout the study. The study showed that coccidiosis affected temperature homeostasis in animals significantly. Body surface temperature decreases significantly (p<0.05). IRT is a promising tool that may provide a more objective an quantitative information about thermal status of animals.