Miracle-dz/UniFAR-FTUs huggingface dataset:
Signal Transduction CYP 1 A 1 Regulates Breast Cancer Proliferation and Survival

Cytochrome P450-1A1 (CYP1A1) is an extrahepatic phase I metabolizing enzyme whose expression is suppressed under physiologic conditions but can be induced by substrates via the aryl hydrocarbon receptor (AhR). Recent studies have shown that the majority of breast cancer tumors constitutively express CYP1A1. These findings led us to test the hypothesis that CYP1A1 promotes breast cancer progression by evaluating the effects of CYP1A1 knockdown on the proliferation and survival of the MCF7 and MDA-MB-231 lines. Independently of estrogen receptor status, CYP1A1 knockdown decreased colony formation, decreased cell proliferation, blocked the cell cycle at G0-G1 associated with reduction of cyclin D1, and increased apoptosis associated with reduction of survivin. CYP1A1 knockdown markedly increased phosphorylation of AMPactivated protein kinase (AMPK) and decreased phosphorylation of AKT, extracellular signal-regulated kinases 1 and 2 (ERK1/2), and 70-kDa ribosomal protein S6 kinase (P70S6K). AMPK inhibition by compound C partially abrogated the proapoptotic effects of CYP1A1 knockdown, suggesting that effects of CYP1A1 knockdown are mediated in part through AMPK signaling. Consistent with CYP1A1 knockdown, pharmacologic reduction of CYP1A1 levels by the phytopolyphenol carnosol also correlated with impaired proliferation and induced AMPK phosphorylation. These results indicate that reduction of basal CYP1A1 expression is critical for inhibition of proliferation, which is not affected by a-naphthoflavone-mediated inhibition of CYP1A1 activity nor modulated by AhR silencing. This study supports the notion that CYP1A1 promotes breast cancer proliferation and survival, at least in part, through suppression of AMPK signaling and that reduction of CYP1A1 levels is a potential strategy for breast cancer therapeutics. Mol Cancer Res; 11(7);
Bacillus iocasae sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin.

A novel bacterial strain S36T was isolated from the deep-sea sediment collected from Pacmanus hydrothermal field, Manus Basin. The strain was Gram-stain-positive, aerobic, rod-shaped, endospore-forming, and motile. It was able to grow at 16-50 °C, pH 6.0-10.0, and in the presence of 0-11 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain S36T was a member of genus Bacillus and shares the highest sequence identity with Bacillus herbersteinensis D-1,5aT (97.0 %). The value of DNA-DNA hybridization between strain S36T and B. herbersteinensis D-1,5aT was 22.8 %. The cell wall diagnostic diamino acid of strain S36T was meso-diaminopimelic acid and the polar lipid profile of strain S36T contained diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The predominant respiratory quinine was MK-7. The major cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The genomic DNA G+C content of strain S36T was 43.0 mol%. On the basis of phylogenetic analysis, DNA-DNA hybridization, and phenotypic characteristics, it was concluded that strain S36T represents a novel species of the genus Bacillus, for which the name Bacillus iocasae sp. nov. was proposed. The type strain is S36T (=KCTC 33864T=DSM 104297T=CGMCC 1.16030T).
Use of spaceborne GPS Navigation sensor for autonomous LEO orbit determination

In this paper, a simple but fairly accurate algorithm, to determine orbit of the Low Earth Orbit (LEO) satellite, in its real time and with low computational burden is reported. This is done by using raw navigation solution provided by GPS Navigation sensor. A fixed step-size Runge-Kutta 4th order numerical integration method is selected for orbit propagation. Both, the Least square and Extended Kalman Filter (EKF) orbit estimation algorithms are developed and the results of the same are compared with each other. The least square algorithm converges after seven iterations. In the case of EKF, the algorithm converges after three iterations. Hence, EKF algorithm satisfies the criterions of low computation burden which is required for autonomous orbit determination.
A flexible, line-based JPEG 2000 decoder for digital cinema

The image compression standard JPEG 2000 proposes a large set of features, useful for today's multimedia applications. Unfortunately, its complexity is greater than older standards. Real-time applications such as digital cinema requires a specific hardware implementation. In this paper, a decoding scheme is proposed with two main characteristics. Firstly, the complete scheme takes place in an FPGA without accessing any external memory, allowing integration in a secured system. Secondly, a customizable level of parallelization allows to satisfy a broad range of constraints, depending on the signal resolution.
Service Demand of Senior for Spa on Wellness Tourism : An Explorative Study

The study is exploring the Spa service demand and features in wellness tourism for seniors. In addition to the senior of respondents, the 50-64 years (pre-senior) of the respondents are also considered to compare the two types of sample features and the differences of Spa service demand . As following constructs the Spa service system design and management of reference. Therefore, this study aimed at Taiwan one of the city's population aged 50 to 64 (potential seniors) and 65 years of age population (seniors) are two groups to investigate. After the statistical analysis of the samples, there are three main findings are shown as follow: (1) By factor analysis, "society /learning", "relaxation", "Spa therapy / functional", "facility", "health information to provide/ consultation " and "healthy environment/diet", the six wellness tourism of spa service demand factors were extracted. (2) 65 years or more of senior in the "society / learning" and "health information to provide / consultation" of demand factors that were significantly higher than the 50 to 64 years of pre-senior. However, 50 to 64 years of pre-senior in "facility" of demand factor that was significantly higher than 65 years or more of Senior. (3) Used Chi-square to test 65 years or more of senior and 50 to 64 years of pre-senior in the demographic features. The results showed that the former has a higher education level and a higher spent on travel.
Transmembrane domains of the syndecan family of growth factor coreceptors display a hierarchy of homotypic and heterotypic interactions

The single-pass transmembrane domains (TMDs) of the syndecan family of cell surface adhesion molecules have been implicated in functional protein–protein interactions. Although each paralog contains a conserved GxxxG dimerization motif, we show here that the syndecan-1 TMD dimerizes weakly, the syndecan-3 and syndecan-4 TMDs each dimerize strongly, and the syndecan-2 TMD dimerizes very strongly. These markedly different levels of self-association suggest that paralog TMDs play different roles in directing functional interactions of each full-length syndecan family member. We further show that each syndecan TMD forms detergent-resistant heteromeric complexes with other paralogs, and that these interactions exhibit selectivity. Although heteromeric interactions among full-length syndecan paralogs have not been reported, we argue that the distinct hierarchy of protein–protein interactions mediated by the syndecan TMDs may give rise to considerable complexity in syndecan function. The demonstration that TMD homodimerization and heterodimerization can be mediated by GxxxG motifs and modulated by sequence context has implications for the signaling mechanisms of other cell surface receptors, including the integrins and the erbB family.
Non-intubated Thoracic Surgery: Wedge Resections for Peripheral Pulmonary Nodules

The feasibility of performing pulmonary resections of peripheral lung nodules has been one of the main objectives of non-intubated thoracic surgery. The aim was to obtain histological characterization and extend a radical intended treatment to oncological patients unfit for general anesthesia or anatomic pulmonary resections. There is mounting evidence for the role of wedge resection in early-stage lung cancer treatment, especially for frail patients unfit for general anesthesia and anatomic resections with nodules, demonstrating a non-aggressive biological behavior. General anesthesia with single lung ventilation has been associated with a higher risk of ventilator-induced barotrauma and volotrauma as well as atelectasis in both the dependent and non-dependent lungs. Nonetheless, general anesthesia has been shown to impair the host immune system, eventually favoring both tumoral relapses and post-operative complications. Thus, non-intubated wedge resection seems to definitely balance tolerability with oncological radicality in highly selected patients. Nonetheless, differently from other non-surgical techniques, non-intubated wedge resection allows for histological characterization and possible oncological targeted treatment. For these reasons, non-intubated wedge resection is a fundamental skill in the core training of a thoracic surgeon. Main indications, surgical tips, and post-operative management strategies are hereafter presented. Non-intubated wedge resection is one of the new frontiers in minimal invasive management of patients with lung cancer and may become a standard in the armamentarium of a thoracic surgeon. Appropriate patient selection and VATS expertise are crucial to obtaining good results.
Microbial Ecology Dynamics during Rye and Wheat Sourdough Preparation

ABSTRACT The bacterial ecology during rye and wheat sourdough preparation was described by 16S rRNA gene pyrosequencing. Viable plate counts of presumptive lactic acid bacteria, the ratio between lactic acid bacteria and yeasts, the rate of acidification, a permutation analysis based on biochemical and microbial features, the number of operational taxonomic units (OTUs), and diversity indices all together demonstrated the maturity of the sourdoughs during 5 to 7 days of propagation. Flours were mainly contaminated by metabolically active genera (Acinetobacter, Pantoea, Pseudomonas, Comamonas, Enterobacter, Erwinia, and Sphingomonas) belonging to the phylum Proteobacteria or Bacteroidetes (genus Chryseobacterium). Their relative abundances varied with the flour. Soon after 1 day of propagation, this population was almost completely inhibited except for the Enterobacteriaceae. Although members of the phylum Firmicutes were present at very low or intermediate relative abundances in the flours, they became dominant soon after 1 day of propagation. Lactic acid bacteria were almost exclusively representative of the Firmicutes by this time. Weissella spp. were already dominant in rye flour and stably persisted, though they were later flanked by the Lactobacillus sakei group. There was a succession of species during 10 days of propagation of wheat sourdoughs. The fluctuation between dominating and subdominating populations of L. sakei group, Leuconostoc spp. , Weissella spp. , and Lactococcus lactis was demonstrated. Other subdominant species such as Lactobacillus plantarum were detectable throughout propagation. As shown by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) analysis, Saccharomyces cerevisiae dominated throughout the sourdough propagation. Notwithstanding variations due to environmental and technology determinants, the results of this study represent a clear example of how the microbial ecology evolves during sourdough preparation.
Replication slippage involves DNA polymerase pausing and dissociation

Genome rearrangements can take place by a process known as replication slippage or copy‐choice recombination. The slippage occurs between repeated sequences in both prokaryotes and eukaryotes, and is invoked to explain microsatellite instability, which is related to several human diseases. We analysed the molecular mechanism of slippage between short direct repeats, using in vitro replication of a single‐stranded DNA template that mimics the lagging strand synthesis. We show that slippage involves DNA polymerase pausing, which must take place within the direct repeat, and that the pausing polymerase dissociates from the DNA. We also present evidence that, upon polymerase dissociation, only the terminal portion of the newly synthesized strand separates from the template and anneals to another direct repeat. Resumption of DNA replication then completes the slippage process.
Distinct signaling of Drosophila chemoreceptors in olfactory sensory neurons

Significance Drosophila is a popular model system for the study of olfaction. However, the physiological properties of its olfactory sensory neurons, both intrinsic and responsive, remain unclear. We have succeeded, for the first time, in patch-clamp recording from targeted Drosophila OSNs, revealing the distinct signaling of odorant receptors (Ors) and ionotropic receptors (Irs). We found that Ir-driven receptor currents did not adapt, whereas Or responses strongly adapted. Surprisingly, although Or adaptation increased odor sensitivity at high odor backgrounds, it reduced odor sensitivity at low backgrounds. Adaptation permeates all senses, and the finding of dynamic gain control by adaptation in Drosophila Or-expressing OSNs sheds light on the understanding of adaptation in other sensory systems. In Drosophila, olfactory sensory neurons (OSNs) rely primarily on two types of chemoreceptors, odorant receptors (Ors) and ionotropic receptors (Irs), to convert odor stimuli into neural activity. The cellular signaling of these receptors in their native OSNs remains unclear because of the difficulty of obtaining intracellular recordings from Drosophila OSNs. Here, we developed an antennal preparation that enabled the first recordings (to our knowledge) from targeted Drosophila OSNs through a patch-clamp technique. We found that brief odor pulses triggered graded inward receptor currents with distinct response kinetics and current–voltage relationships between Or- and Ir-driven responses. When stimulated with long-step odors, the receptor current of Ir-expressing OSNs did not adapt. In contrast, Or-expressing OSNs showed a strong Ca2+-dependent adaptation. The adaptation-induced changes in odor sensitivity obeyed the Weber–Fechner relation; however, surprisingly, the incremental sensitivity was reduced at low odor backgrounds but increased at high odor backgrounds. Our model for odor adaptation revealed two opposing effects of adaptation, desensitization and prevention of saturation, in dynamically adjusting odor sensitivity and extending the sensory operating range.
Residential Electricity Consumption Behavior Mining Based on System Cluster and Grey Relational Degree

In order to improve the utilization of the residential electricity consumption data which contains the information on the user’s electricity consumption habits, a residential electricity consumption behaviors mining algorithm model is constructed. Firstly, according to the attribute, the collected data can be divided into the global data and the phase data, then the appropriate global variables are selected to mine the user’s electricity consumption patterns in the near future on the system clustering algorithm. Based on the theory of grey relational analysis, combing phase data with the power modes to analyze the potential characteristics of residential electricity consumption behaviors deeply that verify the ability of latest power mode to predict household electricity consumption situation in the coming few days and the effect of dominant phase variables on the peak load shifting. Finally, from the actual data of a certain family, the proposed data mining algorithm is testified that it can effectively explore the electricity consumption behavior habits and characteristics of the family.
A Novel Risk Score to Predict In-Hospital Mortality in Patients With Acute Myocardial Infarction: Results From a Prospective Observational Cohort

Objectives The aim of this study was to develop and validate a novel risk score to predict in-hospital mortality in patients with acute myocardial infarction (AMI) using the Heart Failure after Acute Myocardial Infarction with Optimal Treatment (HAMIOT) cohort in China. Methods The HAMIOT cohort was a multicenter, prospective, observational cohort of consecutive patients with AMI in China. All participants were enrolled between December 2017 and December 2019. The cohort was randomly assigned (at a proportion of 7:3) to the training and validation cohorts. Logistic regression model was used to develop and validate a predictive model of in-hospital mortality. The performance of discrimination and calibration was evaluated using the Harrell’s c-statistic and the Hosmer-Lemeshow goodness-of-fit test, respectively. The new simplified risk score was validated in an external cohort that included independent patients with AMI between October 2019 and March 2021. Results A total of 12,179 patients with AMI participated in the HAMIOT cohort, and 136 patients were excluded. In-hospital mortality was 166 (1.38%). Ten predictors were found to be independently associated with in-hospital mortality: age, sex, history of percutaneous coronary intervention (PCI), history of stroke, presentation with ST-segment elevation, heart rate, systolic blood pressure, initial serum creatinine level, initial N-terminal pro-B-type natriuretic peptide level, and PCI treatment. The c-statistic of the novel simplified HAMIOT risk score was 0.88, with good calibration (Hosmer–Lemeshow test: P = 0.35). Compared with the Global Registry of Acute Coronary Events risk score, the HAMIOT score had better discrimination ability in the training (0.88 vs. 0.81) and validation (0.82 vs. 0.72) cohorts. The total simplified HAMIOT risk score ranged from 0 to 121. The observed mortality in the HAMIOT cohort increased across different risk groups, with 0.35% in the low risk group (score ≤ 50), 3.09% in the intermediate risk group (50 < score ≤ 74), and 14.29% in the high risk group (score > 74). Conclusion The novel HAMIOT risk score could predict in-hospital mortality and be a valid tool for prospective risk stratification of patients with AMI. Clinical Trial Registration [https://clinicaltrials.gov], Identifier: [NCT03297164].
Hematologic and Plasma Biochemistry Values in White Storks (Ciconia ciconia)

Abstract Hematologic and plasma biochemistry parameters of the white stork (Ciconia ciconia) were studied. Blood samples were taken from a total of 80 adult white storks kept in captivity in Hungarian zoos and bird repatriation stations, between 2002 and 2006. Hematologic (packed cell volume, 46.3% ± 5.3%; hemoglobin concentration, 127.8 ± 20.4 g/L; red blood cell counts, 2.28 ± 0.35 1012/l/l; white blood cell counts, 21.6 ± 4.2 109/l/l; heterophils, 61.0% ± 9.8% [13.1 ± 3.2 × 109/L]; lymphocytes, 34.3% ± 9.1% [7.4 ± 2.5 × 109/L]; monocytes, 3.44% ± 2.3% [0.78 ± 0.57 × 109/L]; eosinophils 0.75% ± 0.91% [0.16 ± 0.21 × 109/L]; basophils 0.38% ± 0.56% [0.04 ± 0.07 × 109/L]) and plasma biochemistry values (aspartate aminotransferase, 267.5 ± 145.8 U/L; L-γ-glutamyltransferase, 47.6 ± 49.3 U/L; lipase, 70.3 ± 60.6 U/L; creatine kinase, 443.9 ± 182.2 U/L; lactate dehydrogenase, 880.4 ± 293.6 U/L; alkaline phosphatase, 177.5 ± 116.6 U/L; amylase, 917.6 ± 314.3 U/L; glutamate dehydrogenase, 7.3 ± 4.0 U/L; total protein, 45.2 ± 8.1 g/L; uric acid, 459.2 ± 254.3 µmol/L; and bile acids, 46.3 ± 20.5 µmol/L) were determined. The results obtained can be used as reference values, because there are no established values previously reported for adult white storks.
Focused‐ion‐beam fabrication of ZnO nanorod‐based UV photodetector using the in‐situ lift‐out technique

ZnO nanorods and nanowires are promising candidates as new types of high‐sensitivity ultraviolet (UV) photodetectors due to their wide bandgap and large aspect ratio. In this study, single‐crystalline ZnO nanorods were grown on glass substrates by a hydrothermal method. Specific structural, morphological, electrical, and optical measurements were carried out. A single ZnO nanorod‐based photodetector was fabricated using the in‐situ lift‐out technique in a focused ion beam (FIB/SEM) instrument and characterized. With a source wavelength of 370 nm and an applied bias of 1 V, the responsivity of the ZnO nanorod is 30 mA/W. The single ZnO nanorod photodetector exhibits an ultraviolet (UV) photoresponse promising for potential applications as cost‐effective UV detectors. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Disaccharidase Activities in Dyspeptic Children: Biochemical and Molecular Investigations of Maltase-Glucoamylase Activity

Background Maltase-glucoamylase enzyme plays an important role in starch digestion. Glucoamylase deficiency is reported to cause chronic diarrhea in infants, but its role in dyspeptic children is unknown. Methods Glucoamylase and other disaccharidase specific activities were assayed from duodenal biopsy specimens in 44 children aged 0.5–18 years (mean, 10 ± 5 years) undergoing endoscopy to evaluate dyspeptic symptoms. All subjects had normal duodenal histology. Intestinal organ culture was used to evaluate synthesis and processing of maltase-glucoamylase. Sequencing of the maltase-glucoamylase coding region was performed in subjects with low activity or variation of isoform in organ culture. Results Twenty-two of the dyspeptic children had one or more disaccharidases with low specific activity. Twelve subjects (28%) had low activity of glucoamylase. Eight subjects had low activities of glucoamylase, sucrase, and lactase. Low glucoamylase activity was not correlated with the isoform phenotype of maltase-glucoamylase as described by metabolic labeling and sodium dodecyl sulfate electrophoresis. Novel nucleotide changes were not detected in one subject with low glucoamylase activity or in two subjects with variant isoforms of maltase-glucoamylase peptides. Conclusion Twelve of 44 dyspeptic children had low specific activity of duodenal maltase-glucoamylase. Eight of these children had low specific activity of all measured disaccharidases.
Non-fragile mixed LQR/H∞ control problem for linear discrete-time systems with controller uncertainty

This paper considers the discrete-time state feedback non-fragile mixed LQR/H∞ control problem with controller uncertainty. It is assumed that the controller has a time-varying norm-bounded uncertainty. The controller solving the discrete-time non-fragile control problem is designed. Necessary and sufficient conditions for the existence of such a controller are given in terms of an algebraic Riccati equation.
Comparison of rat liver parenchymal and nonparenchymal cells in the activation of promutagens

While the liver consists of both parenchymal cells (PC) and nonparenchymal cells (NPC), virtually all studies on promutagen activation have been performed using PC. To evaluate the comparative roles of PC and NPC in promutagen activation, we cocultivated a cell line generally considered to have an insignificant level of xenobiotic metabolism, Chinese hamster ovary (CHO) cells, with either PC, NPC, or a combination of both. The mixed culture was treated with two promutagens: dimethylnitrosamine (DMN) and 3‐methylcholanthrene (3‐MC). The induction of 6‐thioguanine resistant mutants was evaluated using the well‐established CHO/hypoxanthine‐guanine phosphoribosyl transferase (HGPRT) assay. Activation of promutagens, as indicated by an increase in mutant frequency in CHO cells, was observed only when the PC were present with the CHO cells during the treatment period. No activation was observed with NPC. Coculturing of PC and NPC yielded essentially the same results as PC alone. P‐450 mixed function monooxygenase activity measured by the 7‐ethoxycoumarin‐O‐deethylase assay further substantiates that PC had a significantly higher xenobiotic metabolism activity than NPC. Our study therefore indicates that PC, not NPC, are the major cell population in the liver responsible for the activation of promutagens. © 1992 Wiley‐Liss, Inc.