Miracle-dz/UniFAR-FTUs huggingface dataset:
Secure state estimation and attack reconstruction for cyber-physical system with intermediate and robust observer

An intermediate and robust observer is presented in this article to robustly estimate secure state and reconstruct actuator attack for continuous-time/discrete-time cyber-physical system. First, we propose a general form of cyber-physical system under actuator attack and external disturbance, and then some assumptions are stated in order to facilitate further research. Second, a novel intermediate and robust observer is designed, based on which an augmented error equation is deduced, necessary and sufficient conditions for continuous-time/discrete-time cyber-physical system to obtain gain matrices are derived separately. Third, strict linear matrix inequality conditions are deduced by transforming the original problem into convex optimization problem. Finally, we conduct two simulation experiments with continuous-time and discrete-time unmanned ground vehicle model to show the effectiveness of the proposed secure state estimation and attack reconstruction approach.
Evaluation of Pesticide Residue Dynamics in Lettuce, Onion, Leek, Carrot and Parsley

The dynamics of 32 active substances contained in pesticide formulations (15 fungicides and 17 insecticides) were analyzed in iceberg lettuce, onion, leek, carrot, and parsley. Pesticide residues were monitored from the time of application until harvest. In total, 114 mathematical models of residue dissipation were developed using a first-order kinetic equation. Based on these models, it was possible to predict the action pre-harvest interval (the time between the last pesticide application and crop harvest) needed to attain a targeted action threshold (value significantly lower than the maximum limit) for low-residue vegetable production. In addition, it was possible to determine an action pre-harvest interval based on an action threshold of 0.01 mg kg−1 to produce vegetables intended for zero-residue production. The highest amount of pesticide residues were found in carrot and parsley leaves several days after treatment, and pesticide dissipation was generally slow. Lower amounts were found in leeks and lettuce, but pesticide dissipation was faster in lettuce. According to our findings, it seems feasible to apply reduced pesticide amounts to stay below unwanted residue levels. However, understanding the effectivity of reduced pesticide application for controlling relevant pest organisms requires further research.
Effect of Exogenous Galactose on EPS Production during Bioleaching of Pyrite by Acidithiobacillus ferrooxidans

Extracellular polymeric substances (EPS) play an important role in the attachment of bacteria to sulphide minerals, biofilm formation and efficiency of the bioleaching process. Previous studies have suggested a potential connection between galactose and EPS formation. In this context, the influence of exogenous galactose on EPS formation during the bioleaching of pyrite was studied. In order to fully adapt the microorganism to bioleaching conditions it was performed a total of five consecutive sub cultures, one every fifteen days, taking for each one inocula from previous culture in shake flasks with 200 ml of fermentation medium at 30°C, 200 rpm, 40 gL-1 mineral and an initial pH of 1,8. Assays were performed in a medium supplemented with exogenous galactose (0.25% w/v) and without exogenous galactose (control), both with an initial concentration of ferric sulphate in the first three sub cultures of 5 gL-1, decreasing in the last two sub cultures to 2.5 gL-1. Samples of three cultures in both conditions were analyzed using confocal laser scanning microscopy (CLSM) labelling the cells with propidium iodide and EPS carbohydrates with Wheat Germ Agglutinin (WGA). Samples obtained on the last day of the fifth culture showed that the EPS layer on the particle surface was 5.00 μm3/μm2 in the case of the control condition and 6.10 μm3/μm2 when bioleaching was carried out in the presence of exogenous galactose. Also it was observed that in the fifth sub culture the volumetric productivity of total iron in the control experiment was 0.0065 gL-1.h-1 compared with 0.0076 gL-1.h-1 obtained in presence of galactose. The results reveal that the presence of galactose in the bioleaching solution stimulates EPS's formation and apparently also favour the pyrite bioleaching process.
Effect of Different Dietary Energy Sources on Induction of Fatty Liver-Hemorrhagic Syndrome in Laying Hens

This experiment was conducted to determine the effect of different dietary energy sources on hepatic lipid content and induction of Fatty Liver-Hemorrhagic Syndrome in laying hens. One hundred and fifty Lohmann commercial layers were divided into three groups of fifty birds each. According to a single factorial arrangement, birds were given one of three experimental diets with control(group 1, 2.65Mcal/kg),or high energy diet which was offered with 7% lard (group 2, 3.06Mcal/kg) and offered with 26% cornstarch (group 3, 3.00Mcal/kg). High energy diets decreased feed intake and egg production (p<0.01), average egg weight of group 3 (cornstarch) was decreased (p<0.01); In chicks fed the 7% lard diet or 26% cornstarch ad libitum, hepatic EE (ether extract) and triglyceride were increased significantly, cornstarch could increase hepatic EE, triglyceride and total cholesterol more significant than lard. The average activity of ALT and AST of high energy was increased to be more than 2 times than control treatment of the last three times blood collection. The LDH of high energy diet was increased with no significant difference of the first three times blood collection. In our experiment, the birds fed the 7% lard diets had the largest increase in plasma triglyceride, but there were no significant treatment effects on plasma triglyceride content by cornstarch all the experiment period. The average hemorrhage score of group 2(lard diet) was the highest in the three groups. In chicks fed the 7% lard diet or 26% cornstarch ad libitum had significantly higher MDA values. In the present study, plasma ALT, AST, LDH activities and TC concentration were positively correlated with hepatic TG concentration (p<0.01). The results showed that measurement of enzyme activities indicative of liver damage in birds, particularly AST, ALT, and plasma TC concentration, is a valuable tool in the diagnosis of fatty liver-hemorrhagic syndrome in a flock of layers. These results suggest that dietary carbohydrate can induce FLHS more efficiently that dietary fat, the hens overfeed high fat diets have significantly more liver hemorrhage than high carbohydrate diets.
Selection Response in a Divergent Selection Experiment for Birth Weight Variability in Mice Compared with a Control Line

Simple Summary The divergent selection for birth weight variability was previously proven by the difference between the two selected mice lines, but the comparison with a control line made it possible to measure independently the differential response in each of the low and high birth weight variability lines. In conclusion, the genetic response was much higher for the low variability line than for the high variability line. This was extremely satisfactory given that homogeneity provides advantages in terms of animal welfare and robustness. Abstract Birth weight (BW) in animal production is an economically important trait in prolific species. The laboratory mouse (Mus musculus) is used as an experimental animal because it is considered a suitable model for prolific species such as rabbits and pigs. Two mouse lines were divergently selected for birth weight variability with a third line of non-selected control population of the same origin as the animals starting the experiment. The objective of this study was, therefore, to compare and evaluate the differential response of each line. The animals were from the 17th generation of both low and high BW variability lines of the divergent selection experiment, including in addition animals from the control line. The dataset contained 389 records from 48 litters of the high line, 734 records from 73 litters of the low line, and 574 records from 71 litters of the control line. The studied traits were as follows: the BW, the BW variance, the BW standard deviation, the BW coefficient of variation within-litter, the weaning weight (WW), the litter size at birth and at weaning, the weight gain, and the preweaning survival. The model included the line effect jointly with the parturition number and its interaction, the linear and quadratic LS as covariates except for the LS trait itself when analyzing litter traits, as well as the pup sex when analyzing individual traits. The low line had a lower BW and WW, but a higher litter size, and greater robustness owing to a higher survival at weaning. As a model of livestock animals, the findings from this experiment led to a proposal of selection for pig production that would combine an increase in litter size with higher survival and welfare. Compared with the control line, a much higher response was observed in the low variability line than in the high line, making it extremely satisfactory given that homogeneity provides advantages in terms of animal welfare and robustness.
Retrospectively assessed subjective effects of initial opioid use differ between opioid misusers with opioid use disorder (OUD) and those who never progressed to OUD: Data from a pilot and a replication sample

Attempts to identify opioid users with increased risk of escalating to opioid use disorder (OUD) have had limited success. Retrospectively assessed subjective effects of initial opioid misuse were compared in a pilot sample of opioid misusers (nonmedical use ≤60 times lifetime) who had never met criteria for OUD (N = 14) and heroin‐addicted individuals in treatment for OUD (N = 15). Relative to opioid misusers without a lifetime OUD diagnosis, individuals with OUD reported greater euphoria and other positive emotions, activation, pruritus, and internalizing symptoms. Consistent with these findings, proxy Addiction Research Center Inventory (ARCI) Amphetamine Group, and Morphine Benzedrine Group scale mean item scores were significantly higher in those with OUD. Replication was attempted in opioid misusers with (N = 25) and without OUD (N = 25) who were assessed as part of an ongoing genetic study. We observed similar significant between‐group differences in individual subjective effect items and ARCI scale mean item scores in the replication sample. We, thus confirm findings from prior reports that retrospectively assessed subjective responses to initial opioid exposure differ significantly between opioid users who do, and do not, progress to OUD. Our report extends these findings in comparisons limited to opioid misusers. Additional research will be necessary to examine prospectively whether the assessment of subjective effects after initial use has predictive utility in the identification of individuals more likely to progress to OUD.
Expanding Contraception Access for Women With Opioid-Use Disorder: A Qualitative Study of Opportunities and Challenges

Purpose: As almost nine in ten pregnancies among women with opioid use disorder (OUD) are unintended, expanding access to contraception is an underutilized but potentially effective strategy in increasing reproductive agency and reducing the overall burden of neonatal abstinence syndrome. We aimed to identify where and how contraceptive services could be integrated into existing points-of-contact for women with OUD. Approach: In-depth qualitative interviews. Setting: Three diverse catchment areas in Missouri. Participants: Women with OUD (n = 15) and professional stakeholders (n = 16) representing five types of existing OUD service points: syringe exchange programs, recovery support programs, substance use treatment programs, emergency departments, and Federally Qualified Health Centers. Method: Interviews were audio-recorded, transcribed, and thematically coded using Dedoose software. Results: Six themes emerged as essential components for integrating contraceptive services into existing points-of-contact for women with OUD: (1) reach women with unmet need; (2) provide free or affordable contraception; (3) maximize service accessibility; (4) provide patient-centered care; (5) employ willing, qualified contraceptive providers; and (6) utilize peer educators. Participants affirmed the overall potential benefit of contraceptive service integration and illuminated various opportunities and challenges relevant to each type of existing service point. Conclusion: As health promotion initiatives look to increase access to contraception among women with OUD, these six’ participant-identified components offer essential guidance in selecting advantageous points-of-contact and addressing remaining gaps in services.
Circular RNA expression and association with the clinicopathological characteristics in papillary thyroid carcinoma

Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. Circular RNAs (circRNAs) are a novel class of RNAs, with higher stability and tissue specificity, which may be of value as novel clinical markers. High-throughput RNA sequencing was used to profile the expression of circRNAs in 5 pairs of cancer and normal tissues, and reverse transcription-quantitative PCR (RT-qPCR) analysis was employed to verify the results of the RNA sequencing in 45 cases of PTC. The dysregulated circRNA expression and clinicopathological characteristics were assessed and the potential roles of circRNAs in the cellular miRNA and mRNA network were predicted using bioinformatics analysis. The results demonstrated that, compared with normal tissues, a total of 53 circRNAs were dysregulated in tumour tissues, and 8 circRNAs were validated at the mRNA level (P<0.001 and P<0.01). Among those, the expression of chr5:161330882-161336769- (P=0.015), chr9:22046750-22097364+ (P=0.041) and chr8:18765448-18804898- (P=0.036) were obviously associated with the BRAFV600E mutation, chr12:129699809-129700698- was associated with capsular invasion (P=0.025) and chr5:38523418-38530666- was associated with pT stage (P=0.037) and lymph node metastasis (P=0.002). Therefore, some dysregulated circRNAs were found to be associated with BRAFV600E mutation, capsular invasion, advanced pT stage and lymph node metastasis of PTC, indicating that circRNAs may be involved in tumourigenesis and cancer progression, and they may be putative biomarkers for the diagnosis and evaluation of progression of PTC.
Application of bile acids in drug formulation and delivery

Bile acids are naturally produced in humans and are known to provide human health benefits through their endocrinological, microfloral, metabolic and other åffects that are still to be elucidated. In recent years, there has been a growing interest in using bile acids as absorption enhancers for drug delivery. Bile acids are amphiphilic molecules with a unique ability to facilitate and promote drug permeation through biological membranes. The role of bile acids in promoting drug permeation has been experimentally illustrated in various pharmaceutical formulations including oral, nasal, ocular, buccal, pulmonary and rectal delivery as well as through the blood–brain barrier. Recently, bile acids have drawn attention in the field of drug delivery due to their ability to act as a drug carrier system in the form of mixed micelles, bilosomes and chemical conjugates with drug molecules. Bile acids have demonstrated a unique ability to enhance the epithelial transport of hydrophilic drugs through the paracellular route and that of hydrophobic compounds through both paracellular and transcellular routes. The aim of this review is to discuss various chemical and pharmaceutical aspects of BAs and their potential applications in drug formulation and delivery.
New device with force sensors for laparoscopic liver resection – investigation of grip force and histological damage

Abstract Introduction As the benefits of minimally invasive surgery are recognized, the rate of laparoscopic liver resection (LLR) is rapidly increasing. Liver tissue is fragile compared to tissue of the stomach and colon. In endoscopic and robotic surgery, sufficient tactile sensation is yet to be obtained. Therefore, it is necessary to measure and indicate the grip force of forceps during surgery. We developed a new device consisting of force sensors and investigated its grip force and the resulting histological damage to liver tissue. Material and methods We measured the grip force generated during laparoscopic surgery in pigs using the forceps with pressure sensors developed by us. Throughout the hepatectomy, we measured the grip force generated by the forceps in real time. We investigated the histological damage to the liver caused by using the forceps with different grip forces. Results The subject produced a mean grip force of 1.75 N during the procedures. The maximum grip force was 3.38 N. By grasping the tissues of the liver with forceps, bleeding and destruction of the hepatic lobules were observed in a manner dependent on increasing grip force. Conclusion The new device is necessary for preventing liver damage in laparoscopic hepatic resection.
Effects of fermented rice bran on DEN-induced oxidative stress in mice: GSTP1, LINE-1 methylation, and telomere length ratio.

N-diethylnitrosamine (DEN), a well-known carcinogen, not only induces excessive reactive oxygen species but also suppresses DNA methylation. This study investigated the effect of fermented rice bran (FRB) treatment on DEN-induced oxidative stress through DNA methylation and telomere length analysis. To evaluate the potential protective role of FRB in oxidative stress, two different doses of FRB, DEN, and their combination were administered to mice that were preadapted or not to FRB. Glutathione-S-transferase P1 (GSTP1) methylation levels significantly decreased at 2 and 24 hr after FRB and DEN co-administration in mice with and without pre-adaptation. Moreover, GSTP1 mRNA was upregulated under DEN-induced oxidative stress. Furthermore, changes in long interspersed nuclear element-1 methylation were observed from the viewpoint of genomic instability. In addition, FRB preadapted mice displayed a lower telomere length ratio than the non-adapted mice, suggesting that FRB adaptation offers advantages over the non-adapted conditions in terms of inflammation suppression. PRACTICAL APPLICATIONS: DEN induces excessive ROS, which is associated with oxidative stress on DNA and other cellular components, resulting in inflammation. This study shows that FRB may alleviate DEN-triggered oxidative stress, based on changes in GSTP1, LINE-1 methylation, and telomere length ratios, thereby, revealing the potential of dietary intervention during inflammation. Furthermore, this study furthers the current understanding of DNA methylation mechanisms underlying the antioxidant and anti-inflammatory effects of functional food components. These results indicate that dietary inclusion of FRB may help decrease oxidative DNA damage and its associated inflammation at early stages of a disease.
Characterisation of Ex Vivo Liver Thermal Properties for Electromagnetic-Based Hyperthermic Therapies

Electromagnetic-based hyperthermic therapies induce a controlled increase of temperature in a specific tissue target in order to increase the tissue perfusion or metabolism, or even to induce cell necrosis. These therapies require accurate knowledge of dielectric and thermal properties to optimise treatment plans. While dielectric properties have been well investigated, only a few studies have been conducted with the aim of understanding the changes of thermal properties as a function of temperature; i.e., thermal conductivity, volumetric heat capacity and thermal diffusivity. In this study, we experimentally investigate the thermal properties of ex vivo ovine liver in the hyperthermic temperature range, from 25 °C to 97 °C. A significant increase in thermal properties is observed only above 90 °C. An analytical model is developed to model the thermal properties as a function of temperature. Thermal properties are also investigated during the natural cooling of the heated tissue. A reversible phenomenon of the thermal properties is observed; during the cooling, thermal properties followed the same behaviour observed in the heating process. Additionally, tissue density and water content are evaluated at different temperatures. Density does not change with temperature; mass and volume losses change proportionally due to water vaporisation. A 30% water loss was observed above 90 °C.
Development of a Winged Tine to Relieve Mining‐Related Soil Compaction after Bauxite Mining in Western Australia

Deep ripping, using a conventional chisel‐tine to a nominal depth of 1.4 m, was introduced to Alcoa’s operations on the Darling Plateau in 1969 following windthrow of a number of trees. Although research into mine floor compaction showed that this ripping depth was sufficient to remove the mining compaction, tillage studies showed that plastic failure around the point of a conventional tine ripping to this depth was actually adding to deep compaction rather than removing it. To improve tillage at depth, wing‐like structures were attached to the shank of a conventional chisel‐tine directly behind the point. These wings lift and till the soil across a broad front. Wingspans between 0.75 and 1.8 m were tested, and a final design of 1.8‐m wingspan adopted for ripping during the 1980s and 1990s. Availability of suitable steels in the late 1990s allowed the winged tines to be fabricated from scratch, enabling the fitting of wear plates to almost completely eliminate shank wear and breakage problems, though these additions have resulted in some loss of tillage efficiency. Modification of the bulldozer ripping box is highly desirable for ripper design to be improved further, particularly to overcome lateral sliding when contour ripping is undertaken on steep slopes. An alternative is to divide the ripping process in two: a pre‐rip up and down the slope followed by shallow ripping on contour. A two‐step ripping process is now being used at Alcoa’s Western Australian bauxite mines.