Miracle-dz/UniFAR-FTUs huggingface dataset:
Resistência de espécies de Mycobacterium a diferentes condições de stress relacionadas com tratamentos de água

V Lista Tabelas . VIII Lista de Figuras . IX Lista de Abreviaturas. XII 1. Introdução . 1 1.1. Perspetiva Histórica . 1 1.2. Género Mycobacterium . 2 1.3. Epidemiologia . 3 1.4. Sistemas de distribuição de água e a presença de microrganismos . 4 1.5. Parede Celular . 7 1.6. Biofilmes . 9 2. Objetivos . 10 3. Materiais e Métodos . 10 3.1. Crescimento dos isolados de MNT em estudo . 10 3.2. Extração DNA . 10 3.3. Identificação dos isolados de MNT em estudo . 10 3.4. Ensaio de crescimento de isolados a diferentes temperaturas . 13 3.4.1. Inoculação de isolados de MNT . 13 3.4.2. Quantificação da concentração total de proteínas (μg/mL) das células bacterianas . 13 3.5. Ensaio de crescimento de isolados a diferentes pHs . 14 3.5. 1. Inoculação de isolados de MNT . 14 3.5. 2. Quantificação da concentração total de proteínas (μg/mL) das células bacterianas . 14 3.6. Ensaio de crescimento a diferentes concentrações de Cloro . 15 3.7. Ensaio de crescimento em condições de privação de nutrientes . 15 3.7. 1. Produção de ATP . 15 3.7. 2. Microscopia de Fluorescência Auramina-Rodamina . 16 Resistência de espécies de Mycobacterium a diferentes condições de stress relacionadas com o tratamento de água VII 3.7. 3. Ensaio PCR Tempo Real . 16 3.8. Permeação através de superfícies . 16 4. Tratamento de Resultados . 17 4.1. Ensaio de crescimento de isolados a diferentes temperaturas . 17 4.1.1. Inoculação de isolados de MNT . 17 4.1.2. Quantificação da concentração total de proteínas (μg/mL) das células bacterianas . 20 4.2. Ensaio de crescimento de isolados a diferentes pHs . 25 4.2. 1. Inoculação de isolados de MNT . 25 4.2. 2. Quantificação da concentração total de proteínas (μg/mL) de células bacterianas . 28 4.3. Crescimento a diferentes concentrações de cloro . 34 4.4. Crescimento em condições de privação de nutrientes . 37 4.4.1. Forma das células – Microscopia . 37 4.4.2. Produção de ATP . 42 4.4.3. Monitorização da concentração total de bactérias (UG/mL) . 42 4.5. Migração através de diferentes tipos de solos . 45 5. Discussão . 47 6. Conclusão . 48 7. Referências bibliográficas . 49 Resistência de espécies de Mycobacterium a diferentes condições de stress relacionadas com o tratamento de água
Towards open-ended VQA models using transformers

In this work, we introduce a new architecture to address the Visual Question Answering problem, an open field of research in the NLP and Vision community. In the last few years, with the advent of Deep Learning and the exponential growth of computing power, researches came up with brilliant solutions to tackle the problem. However, most of the related work share a standard limitation: the number of possible answers is usually restricted to a limited set of candidates, limiting the power of such models. In this work, we describe a new architecture that employs new state-of-the-art language models, such as the Transformer, to generate open-ended answers. In the end, our contribution to the scientific community lies in a new approach that allows VQA systems to generate unconstrained answers. First, we introduce the necessary background as well as the most critical computational models to deal with text and images. Ultimately, we show that our architecture compares well with other VQA models, setting a new baseline for future work.
Discovery, Bioactivity Evaluation, Biosynthetic Gene Cluster Identification, and Heterologous Expression of Novel Albofungin Derivatives

The crude extract of Streptomyces chrestomyceticus exhibited strong and broad activities against most “ESKAPE pathogens. ” We conducted a comprehensive chemical investigation for secondary metabolites from the S. chrestomyceticus strain and identified two novel albofungin (alb) derivatives, i.e., albofungins A (1) and B (2), along with two known compounds, i.e., albofungin (3) and chloroalbofungin (4). The chemical structures of the novel compounds were elucidated using HRMS, 1D and 2D NMR, and electronic circular dichroism spectroscopy. The draft genome of S. chrestomyceticus was sequenced, and a 72 kb albofungin (alb) gene cluster with 72 open reading frames encoding type II polyketide synthases (PKSs), regulators, and transporters, and tailoring enzymes were identified using bioinformatics analysis. The alb gene cluster was confirmed using the heterologous expression in Streptomyces coelicolor, which successfully produced the compounds 3 and 4. Furthermore, compounds 1–4 displayed remarkable activities against Gram-positive bacteria and antitumor activities toward various cancer cells. Notably, compounds 1 and 3 showed potent activities against Gram-negative pathogenic bacteria. The terminal deoxynucleotidyl transferase (dUTP) nick-end labeling and flow cytometry analysis verified that compound 1 inhibited cancer cell proliferation by inducing cellular apoptosis. These results indicated that albofungins might be potential candidates for the development of antibiotics and antitumor drugs.
Early-Life Circumstances and Adult Locus of Control: Evidence from 46 Developing Countries

Early-life circumstances have a long-term impact on adult outcomes such as health, wealth, and happiness. Using exogenous variation in weather conditions across 46 developing countries over time, this study examines the impact of experiencing weather shocks in childhood on adult non-cognitive skills, namely, locus of control. The results show that those who experienced rainfall shortage before age five are more likely to believe that they cannot control their life outcomes. However, the impact diminishes by their early forties. This study also demonstrates the negative impact of weather shocks on voting behavior. Finally, underlying mechanisms for this relationship are explored.
Implikasi WORK ENGAGEMENT, Fun at work, DAN JOB SATISFACTION TERHADAP ORGANIZATIONAL CITIZENSHIP BEHAVIOR PADA KARYAWAN ATRIA HOTEL GADING SERPONG

Atria Hotel Gading Serpong berdiri sejak tahun 2010 di bawah naungan Parador Hotels & Resorts. Atria Hotel Gading Serpong merupakan hotel bintang empat berstandar internasional yang berkomitmen dengan standar internasional untuk melayani kebutuhan pasar lokal di bidang konferensi dan perjalanan dinas. Tujuan penelitian ini adalah untuk mengetahui pengaruh fun at work, work engagement, dan job satisfaction terhadap OCB pada karyawan Atria Hotel Gading Serpong. Metode penelitian yang digunakan adalah metode penelitian kuantitatif dengan metode pengumpulan data menggunakan kuesioner. Teknik pengambilan sampel dilakukan dengan teknik probability sampling dengan metode proportionate stratified sampling dan menggunakan rumus slovin. Data-data yang diperoleh kemudian diolah menggunakan software IBM SPSS Statistic versi 25. Hasil yang dicapai dalam penelitian ini menunjukkan bahwa work engagement, dan job satisfaction memiliki pengaruh positif terhadap OCB, sedangkan fun at work tidak memiliki pengaruh terhadap OCB pada karyawan Atria Hotel Gading Serpong. Kata kunci : work engagement, fun at work, job satisfaction, OCB
Higher order mode dielectric resonator antenna excited using microstrip line

In this paper, the square-shaped dielectric resonator antenna (DRA) operating on higher order (𝑇𝐸𝛿13) mode for the fifth generation (5G) communication applications is presented. The proposed DR antenna is excited by using a microstrip feed line and designed at the operating frequency of 28 GHz. The Rogers RT/Duroid 5880 material having a thickness of 0.254mm and a dielectric constant of 2.2 is used for the substrate. The commercial CST microwave studio (CST MWS) is used for the optimization and simulation of the antenna design. The reflection coefficient, antenna gain, radiation efficiency, VSWR and radiation pattern are studied. A -10dB bandwidth of 4.6% (1.3 GHz) for VSWR<2 with a gain of 5 dBi and radiation efficiency of 89%. The proposed antenna design is suitable for future 5G wireless communication applications. In this paper, the square-shaped dielectric resonator antenna (DRA) operating on higher order ( TE δ 13 ) mode for the fifth generation (5G) communication applications is presented. The proposed DR antenna is excited by using a microstrip feed line and designed at the operating frequency of 28 GHz. The Rogers RT/Duroid 5880 material having a thickness of 0.254mm and a dielectric constant of 2.2 is used for the substrate. The commercial CST microwave studio (CST MWS) is used for the optimization and simulation of the antenna design. The reflection coefficient, antenna gain, radiation efficiency, VSWR and radiation pattern are studied. A -10dB bandwidth of 4.6% (1.3 GHz) for VSWR<2 with a gain of 5 dBi and radiation efficiency of 89%. The proposed antenna design is suitable for future 5G wireless communication applications.
Brain connectivity is modularly represented in the genome

The network organization of human brain functional connectivity is strikingly similar to that of inter-regional gene coexpression. Yet, whether gene coexpression explains functional connectivity with an accuracy that is equivalent across the cortex, or whether different sets of genes explain distinct aspects of functional connectivity remains unknown. Furthermore, it is unclear how the relationship between gene coexpression and brain connectivity might change over development, relate to heritability, and track behavior. Here, we leverage several large multimodal data sets to demonstrate that different sets of genes statistically account for different aspects of brain network architecture. Critically, gene coexpression is more strongly related to functional connectivity than to structural connectivity. Brain regions whose connections are well fit by gene coexpression also tend to have connections whose strengths are commonly shared across humans, co-vary with behavior, and display stereotyped development over adolescence. In contrast, brain regions whose connections are not well fit by gene coexpression tend to connect diverse functional network modules and be strongly heritable. Our results lend support to the notion that functional connectivity is modularly represented in the genome and mediates between genotypes and cognitive phenotypes.
Grain Yield and Quality of Organic Crops Grown under Reduced Tillage and Diversified Sequences

793 The area devoted to organic agricultural production has grown significantly worldwide over the past decade, despite crop yields being reported to be lower with organic than with conventional management practices (Berner et al., 2008; Campiglia et al., 2015; Ponisio et al., 2015). Significant increases in organic production have also occurred in western Canada (Organic Agriculture Centre of Canada, 2017). In southern Manitoba, Canada, Entz et al. (2005) reported that grain yields of flax (Linum usitatissimum L.) ranged from an average of 20 to 70% lower with organic vs. conventional management. Similarly, Benaragama et al. (2016a) reported that in west-central Saskatchewan, Canada organic management reduced the grain yield of cereal, pulse and oilseed crops by 32 and 35% compared to conventional systems that used reducedand high-inputs, respectively. Further, Entz et al. (2001) reported, based on a survey of organic farms in eastern regions of the Canadian prairies (1991 to 1996), that organic grain and forage yields ranged from one-half to almost double the conventional yields. In Maryland, Cavigelli et al. (2008) also reported that yields of corn (Zea mays L.) and soybean (Glycine max L. Merr.) were generally higher in conventional than organic systems, but there were no consistent differences in the yield of wheat (Triticum aestivum L.) among the management systems. Under the dry conditions of Montana, winter wheat grown in an organic system had similar or greater grain productivity and superior grain quality compared with well-fertilized winter wheat grown in a no-till cropping system (Miller et al., 2008). These highly variable yield and quality differences being reported between organic and non-organic systems appear to depend on the specific management practices employed and the site characteristics (Campiglia et al., 2015; Seufert et al., 2012). Differences in environmental conditions primarily affect weed infestations and nitrogen supply in organic systems which, in turn, influence crop yields (Campiglia et al., 2015). Benaragama et al. (2016a) determined that organic systems had 7× and 4× greater weed density and 32 and 35% lower yields than conventional systems that used reducedor high-inputs, respectively, but the higher weed density over time did not result in a continuous decline in crop yields in the organic systems. However, Benaragama et al. (2016b) also showed that in the absence Grain Yield and Quality of Organic Crops Grown under Reduced Tillage and Diversified Sequences
The Three-dimensional Electromagnetic Simulation Analysis Of Amorphous Ferromagnetic Microwires

Amorphous microwires cast from ferromagnetic Fe-based alloys have very unique magneto-anisotropic and high frequency impedance properties that make them very attractive for power electronics applications. These effects are explored for the creation of new inductors and transformers. In this work, the focus is on the study of magnetic coupling by anisotropic magnetization. The results confirm the magnetization is angle dependent, in which the easy magnetic axis along the z axis exhibits anisotropic properties and exhibits isotropic properties in the x and y axes. Using 3D finite element method (FEM) simulations, this research compares magnetic microwire to ferrite and metglas cores when used in AC and DC applications. Bobbin and toroidal inductors are analyzed. Detailed information is given on the geometric setup, assumptions made, and results obtained by the software and analytic discussions. Concluding suggestions are made for future work.
A Review on Azole Derivatives as Potent Anticancer Agents

Cancer a disease where anomalous cells divide irrepressibly and abolish body tissue with potential to invade or extent to other parts of the body which acknowledged as metastasis. Although substantial advancement in chemotherapy, but the delinquent of drug resistance enforced the search of new class of drugs with more efficacy. Azoles are five-membered heterocyclic system displayed numerous pharmacological activity proved the future prospective in pharmaceutical industry. This review summarizes the latest development of azoles as novel chemotherapeutic agents with improved therapeutic competency.
PENERAPAN MODEL UTAUT2 UNTUK MENJELASKAN BEHAVIORAL INTENTION DAN USE BEHAVIOR PENGGUNAAN MOBILE BANKING DI KOTA MALANG

Penelitian ini bertujuan untuk mengetahui penerimaan dan pengunaan mobile banking di Kota Malang dengan menggunakan model UTAUT2. Mobile banking adalah sebuah layanan yang disediakan oleh bank untuk mendukung berbagai macam transaksi perbankan. Jenis penelitian ini adalah explanatory research yang menjelaskan hubungan dan pengaruh antara satu variabel dengan variabel lainnya melalui pengajuan hipotesis. Penelitian ini menggunakan sampel sebanyak 150 responden dengan penyebaran kuesioner online. Sampel terdiri dari responden yang menggunakan mobile banking. Analisis data dalam penelitian ini menggunakan analisis jalur (path analysis) dengan menggunakan program SmartPLS 3.0. Hasil pengujian terhadap kedelapan hipotesis dapat disimpulkan bahwa performance expectancy, effort expectancy, social influence, hedonic motivation, dan price value memiliki pengaruh signifikan terhadap behavioral intention. Facilitating condition, habit dan behavioral intention memiliki pengaruh signifikan terhadap use behavior. Kata Kunci: UTAUT2, mobile banking, behavioral intention, use behavior
The NuA4 acetyltransferase and non-canonical chromatin-associated MRN/ATM activity temporally define senescence-associated secretory programs

Senescent cells display senescence-associated (SA) phenotypic programs such as proliferation arrest (SAPA) and secretory phenotype (SASP), which mediate their impact on tissue homeostasis. Curiously, senescence-inducing persistent DNA double-strand breaks (pDSBs) cause an immediate DNA damage response (DDR) and SAPA, but SASP requires days to develop, suggesting it requires additional molecular events. Here, we show that pDSBs provoke delayed recruitment of MRN/ATM and KAT5/TRRAP (NuA4/Tip60) complexes to global chromatin. This coincided with activating histone marks on up-regulated SASP genes, whereas depletion of these complexes compromised the SASP. Conversely, histone deacetylase inhibition triggered accelerated MRN/ATM/Tip60-dependent SASP without pDSBs, interlacing acetylation and non-canonical DNA damage-independent, low-level DDR activity in SASP maturation. DDR/acetylation regulation of SA programs is preserved in human cancer cells, suggesting novel targets for modifying treatment-induced SA phenotypes. We propose that delayed chromatin recruitment of acetyltransferases cooperates with non-canonical DDR signaling to ensure SASP activation only in the context of senescence, and not in response to a transient DNA damage-induced proliferation arrest. HIGHLIGHTS (4 MAX, 85 CHARACTERS EACH) -Tip60 complex cooperate with non-canonical DDR activity to trigger paracrine signals -Low-level chromatin-associated ATM activity is required for the SASP -The intensity and temporal dynamics of the SASP can be manipulated eTOC blurb (40 words) A temporal ballet between DNA damage response ATM/MRN and NuA4/Tip60 complexes control senescence-associated paracrine signals that are important during aging and cancer therapy, suggesting that DNA damage responses, acetylation, and chromatin remodeling can be exploited to manipulate senescence and benefit health.
Vitamin B17 Ameliorates Methotrexate-Induced Reproductive Toxicity, Oxidative Stress, and Testicular Injury in Male Rats

Methotrexate (MTX; 4-amino-10-methylfolic acid) is a folic acid reductase inhibitor used to treat autoimmune diseases and certain types of cancer. Testicular toxicity resulting from MTX is a significant side effect that may cause subsequent infertility. The present study was conducted to examine the ameliorating effects of vitamin B17 (VitB17) against testicular toxicity induced by MTX in male rats. A total of 50 male albino rats were equally divided into five groups [control group; vitamin B17 group (VitB17) administered VitB17 only; methotrexate group administered MTX only; cotreated group, (VitB17+MTX) and posttreated group (MTX+VitB17)]. In methotrexate group (MTX), a significant decrease was observed in body weight and the testicular weight, as well as the levels of plasma testosterone, luteinizing hormone and follicle-stimulating hormone compared with control. The sperm count, viability, morphology index, total motility, and progressive motility also decreased in MTX rats compared with control. Furthermore, the levels of reduced glutathione, catalase, and superoxide dismutase, as well as proliferating cell nuclear antigen protein expression, in the testicular tissue decreased in MTX compared with control. In addition, MTX caused a significant increase in DNA and tissue damage compared with control. However, VitB17 ameliorated these effects, indicating that it has a preventative and curative effect against MTX-induced reproductive toxicity in male rats. The protective effect of VitB17 may be associated to its antioxidant properties as it possibly acts as a free-radical scavenger and lipid peroxidation inhibitor, as well as its protective effect on the levels of GSH, SOD, and CAT.
Effectiveness of dexamethasone injection in the pterygomandibular space before and after lower third molar surgery

Background Previous studies have investigated the effects of dexamethasone injections into the pterygomandibular space and compared them to those of controls; however, the effects of dexamethasone injections before and after lower third molar surgery on postoperative complications have not been studied. This research investigated the postoperative sequelae of dexamethasone injections before and after surgery into the pterygomandibular space. The aim of this study was to evaluate the effects of preoperative and postoperative injections of 4 mg of dexamethasone into the pterygomandibular space on postoperative pain, facial swelling, and the restriction of mouth opening following lower third molar surgical removal. Methods Twenty-seven participants with bilateral symmetrical lower impacted third molars were included in this study. Each participant was randomly allocated to one of two groups. Group A received injections of 1 ml dexamethasone (4 mg/mL) and 1 mL placebo into the pterygomandibular space before and after surgery, respectively. Group B received the same doses of placebo before surgery and dexamethasone after surgery. Results A significant restriction of mouth opening on the second postoperative day was observed in both groups. Nonetheless, the postoperative restriction of mouth opening, facial swelling, postoperative pain, and analgesic consumption after lower third molar surgical removal were not significantly different in the two groups. Conclusions Regardless of the time of administration, dexamethasone injections into the pterygomandibular space resulted in satisfactory control of the postoperative sequelae of the mandibular third molar surgical removal.
Synchronization of Sensory Gamma Oscillations Promotes Multisensory Communication

Abstract Rhythmic neuronal activity in the gamma range is a signature of cortical processing and its synchronization across distant sites has been proposed as a fundamental mechanism of network interactions. While this has been shown within sensory streams, we tested whether cross talk between the senses relies on similar mechanisms. Direct sensory interactions in humans (male and female) were studied with a visual–tactile amplitude matching paradigm. In this task, congruent stimuli are associated with behavioral benefits, which are proposed to be mediated by increased binding between sensory cortices through coherent gamma oscillations. We tested this hypothesis by applying 4-in-1 multi-electrode transcranial alternating current stimulation (tACS) with 40 Hz over visual and somatosensory cortices. In phase stimulation (0°) was expected to strengthen binding and thereby enhance the congruence effect, while anti-phase (180°) stimulation was expected to have opposite effects. Gamma tACS was controlled by alpha (10 Hz) and sham stimulation, as well as by applying tACS unilaterally while visual–tactile stimuli were presented lateralized. Contrary to our expectations, gamma tACS over the relevant hemisphere delayed responses to congruent trials. Additionally, reanalysis of EEG data revealed decoupling of sensory gamma oscillations during congruent trials. We propose that gamma tACS prevented sensory decoupling and thereby limited the congruence effect. Together, our results favor the perspective that processing multisensory congruence involves corticocortical communication rather than feature binding. Furthermore, we found control stimulation over the irrelevant hemisphere to speed responses under alpha stimulation and to delay responses under gamma stimulation, consistent with the idea that contralateral alpha/gamma dynamics regulate cortical excitability.