Miracle-dz/UniFAR-FTUs huggingface dataset:
Salinity Effects on Seed Germination and Plant Growth of Guar

The potential of guar [Cyamopsis tetragonoloba (L.) Taub. ] as a feasible crop for saline soils was tested in this study by evaluating seed germination of 42 accessions of guar in sodium chloride (NaCI) solution. The seeds were germinated in 200 mM NaCI or double-distilled water (control). There were significant differences in salt tolerance among the accessions tested. Percentages of seeds germinating in the salt solution ranged from 7.7 to 90.3% of the control, with the higher percentages suggesting greater salt tolerance. Twelve of the forty-two accessions were selected to study the effect of salinity on plant growth. Significant differences were obtained between the accessions for seed yield per pod, but not for seed yield per plant, plant height, branch number, and pod number (percentage of control). The presence of variation in the germplasm suggests some potential for selecting cultivars with increased salt tolerance. Salinity studies should include both germination and plant trials, because germination assays for salt tolerance do not necessarily predict plant growth and yield in saline soils.
Infusions of Herbal Blends as Promising Sources of Phenolic Compounds and Bioactive Properties

Several plants have been used for medicinal applications and have been traditionally consumed as decoctions and infusions. Although some herbs are used alone as a beverage, they are often blended in mixtures to maximize their effects. Herein, the nutritional characterization of six infusions from herbal blends was evaluated using the official methods of analysis (AOAC international). A further characterization of the individual phenolic profile was also performed by HPLC-DAD/ESI-MSn, and finally bioactive potential was determined by evaluating the antioxidant, cytotoxic, anti-inflammatory, and antimicrobial activities of each blend. The wide variety of plants in each sample led to variability in the results for all analyzed parameters. However, blends containing 15% Laurus nobilis L. and 15% Juglan regia L. in their composition showed higher sugar content and energy contribution; higher concentration of phenolic compounds (phenolic acids and flavonoids); greater antioxidant, cytotoxic, and anti-inflammatory capacity; and also better antimicrobial effects against all the tested bacterial and fungal strains. Further studies will be necessary to evaluate the real synergistic effects that these two species show in the presence of other plants, and to evaluate their potential for application in various food, pharmaceutical, and nutraceutical products as infusion preparations.
Surgical Indications and Techniques for Adrenalectomy

Indications for adrenalectomy are malignancy suspicion or malignant tumors, non-functional tumors with the risk of malignancy and functional adrenal tumors. Regardless of the size of functional tumors, they have surgical indications. The hormone-secreting adrenal tumors in which adrenalectomy is indicated are as follows: Cushing’s syndrome, arises from hypersecretion of glucocorticoids produced in fasciculata adrenal cortex, Conn’s syndrome, arises from an hypersecretion of aldosterone produced by glomerulosa adrenal cortex, and Pheochromocytomas that arise from adrenal medulla and produce catecholamines. Sometimes, bilateral adrenalectomy may be required in Cushing’s disease due to pituitary or ectopic ACTH secretion. Adenomas arise from the reticularis layer of the adrenal cortex, which rarely releases too much adrenal androgen and estrogen, may also develop and have an indication for adrenalectomy. Adrenal surgery can be performed by laparoscopic or open technique. Today, laparoscopic adrenalectomy is the gold standard treatment in selected patients. Laparoscopic adrenalectomy can be performed transperitoneally or retroperitoneoscopically. Both approaches have their advantages and disadvantages. In the selection of the surgery type, the experience and habits of the surgeon are also important, along with the patient’s characteristics. The most common type of surgery performed in the world is laparoscopic transabdominal lateral adrenalectomy, which most surgeons are more familiar with. The laparoscopic anterior transperitoneal approach is the least preferred laparoscopic method in adrenalectomy. Retroperitoneal laparoscopic adrenalectomy can be performed with a posterior or lateral approach. In addition to conventional laparoscopy, laparoscopic surgery is robot-assisted, which can be administered by transperitoneal or retroperitoneal approach. In addition, conventional or robot-assisted laparoscopic adrenalectomy can be performed transabdominally or retroperitoneally using the single-port method. Today, partial adrenalectomy can be performed using laparoscopic techniques in bilateral adrenal masses, hereditary diseases with the risk of developing multiple adrenal tumors, and solitary masses of the adrenal gland. Open surgery is indicated in the case of malignancy or suspected malignancy and large tumors when laparoscopic surgery is contraindicated. The risk of conversion to open surgery is low (approximately 5%). The open transperitoneal anterior approach is the most common open intervention, especially in large tumors with malignancy or suspected malignancy. This procedure can be performed using a midline incision, bilateral or unilateral subcostal incision, Makuuchi or modified Makuuchi incision. Thoracoabdominal incision may be required, especially in the removal of large malignant lesions as a block. The open retroperitoneal approach can be applied posteriorly or laterally.
Plasma Metabolomics Profiles were Associated with the Amount and Source of Protein Intake: A Metabolomics Approach within the PREDIMED Study.

SCOPE The plasma metabolomics profiles of protein intake has been rarely investigated. We aimed to identify the distinct plasma metabolomics profiles associated with overall intakes of protein as well as with intakes from animal and plant protein sources. METHODS AND RESULTS Cross-sectional analysis using data from 1,833 participants at high risk of cardiovascular disease. Plasma metabolomics analysis was performed using LC-MS. Associations between 385 identified metabolites and the intake of total, animal protein (AP) and plant protein (PP), and plant-to-animal ratio (PR) were assessed using elastic net continuous regression analyses. A double 10-cross-validation (CV) procedure was used and Pearson correlations coefficients between multi-metabolite weighted models and reported protein intake in each pair of training-validation datasets were calculated. A wide set of metabolites was consistently associated with each protein source evaluated. These metabolites mainly consisted of amino acids and their derivatives, acylcarnitines, different organic acids and lipid species. Few metabolites overlapped among protein sources (i.e. C14:0 SM, C20:4 carnitine, GABA and allantoin) but none of them towards the same direction. Regarding AP and PP approaches, C20:4 carnitine and dimethylglycine were positively associated with PP but negatively associated with AP. However, allantoin, C14:0 SM, C38:7 PE plasmalogen, GABA, metronidazole and trigonelline (N-methylnicotinate) behaved contrary. Ten-CV Pearson correlations coefficients between self-reported protein intake and plasma metabolomics profiles ranged from 0.21 for PR to 0.32 for total protein. CONCLUSIONS Different sets of metabolites were associated with total, animal and plant protein intake. Further studies are needed to assess the contribution of these metabolites in protein biomarkers' discovery and prediction of cardiometabolic alterations. This article is protected by copyright. All rights reserved.
Effects of Work and Recovery Duration and Their Ratio on Cardiorespiratory and Metabolic Responses During Aerobic Interval Exercise

Abstract Myrkos, A, Smilios, I, Zafeiridis, A, Iliopoulos, S, Kokkinou, EM, Douda, H, and Tokmakidis, SP. Effects of work and recovery duration and their ratio on cardiorespiratory and metabolic responses during aerobic interval exercise. J Strength Cond Res 36(8): 2169–2175, 2022—This study examined the effect of work and recovery durations and of work-to-rest ratio (WRR) on total exercise time and oxygen consumption (V̇o2max), on exercise time above 80, 90, and 95% of V̇o2max and HRmax, and on blood lactate concentrations during aerobic interval exercise. Twelve men (22.1 ± 1 year) executed, until exhaustion, 4 interval protocols at an intensity corresponding to 100% of maximal aerobic velocity. Two protocols were performed with work bout duration of 120 seconds and recovery durations of 120 (WRR: 1:1) or 60 seconds (WRR: 2:1), and 2 protocols with work bout duration of 60 seconds and recovery durations of 60 (WRR: 1:1) or 30 seconds (WRR: 2:1). When compared at equal exercise time, total V̇o2 and exercise time at V̇o2 above 80, 90, and 95% of V̇o2max were longer (p < 0.05) in 120:120, 120:60 and 60:30 vs. the 60:60 protocol. When analyzed for total exercise time (until exhaustion), total V̇o2 was higher (p < 0.01) in the 60:60 compared with all other protocols, and in the 120:120 compared with 120:60. Exercise time >95% of V̇o2max and HRmax was higher (p < 0.05) in the 120:120 vs. the 60:60 protocol; there were no differences among protocols for exercise time >90% of V̇o2max and HRmax. Blood lactate was lower (p < 0.05) in the 60:60 compared with all other protocols and in the 60:30 vs. the 120:60. In conclusion, when interval exercise protocols are executed at similar effort (until exhaustion), work and recovery durations do not, in general, affect exercise time at high oxygen consumption and HR rates. However, as work duration decreases, a higher work-to-recovery ratio (e.g., 2:1) should be used to achieve and maintain high (>95% of maximum) cardiorespiratory stimulus. Longer work bouts and higher work-to-recovery ratio seem to activate anaerobic glycolysis to a greater extent, as suggested by greater blood lactate concentrations.
Comprehensive analysis of aberrantly expressed long non-coding RNAs, microRNAs, and mRNAs associated with the competitive endogenous RNA network in cervical cancer

Cervical cancer is a common malignant disease that poses a serious health threat to women worldwide. Growing research efforts have focused on protein-coding and non-coding RNAs involved in the tumorigenesis and prognosis of various types of cancer. The potential molecular mechanisms and the interaction among long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and mRNAs require further investigation in cervical cancer. In the present study, lncRNA, miRNA, and mRNA expression profiles of 304 primary tumor tissues from patients with cervical cancer and 3 solid normal tissues from The Cancer Genome Atlas (TCGA) dataset were studied via RNA sequencing (RNA-seq). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using R package clusterProfiler to annotate the principal functions of differentially expressed (DE) mRNAs. Kaplan-Meier analysis was also conducted to investigate the effects of DElncRNAs, DEmiRNAs, and DEmRNAs on overall survival. A total of 2,255 mRNAs, 133 miRNAs, and 150 lncRNAs that were differentially expressed were identified with a threshold of P<0.05 and |fold change (FC)|>2. Functional enrichment analysis indicated that DEmRNAs were enriched in cancer-associated KEGG pathways. Furthermore, 255 mRNAs, 15 miRNAs, and 12 lncRNAs that were significantly associated with overall survival in cervical carcinoma were also identified. Importantly, an miRNA-mediated competitive endogenous RNA (ceRNA) network was successfully constructed based on the expression profiles of DElncRNAs and DEmRNAs. More importantly, it was found that the lncRNA EPB41L4A-AS1 may function as a pivotal regulator in carcinoma of the uterine cervix. Taken together, the present study has provided novel insights into investigating the potential mechanisms underlying tumorigenesis, development, and prognosis of cervical cancer, and presented new potential avenues for cancer therapeutics.
Macular Abnormalities Associated With 5α-Reductase Inhibitor.

Importance The neuroprotective action of sex hormones has been described. Data on the association between 5α-reductase inhibitor (5-ARI), a male sex hormone antagonist, and macular abnormalities are lacking to date. Objective To assess the association between the use of 5-ARI for treatment of benign prostate hypertrophy and/or androgenic alopecia in men and macular abnormalities on optical coherence tomography imaging. Design, Setting, and Participants This retrospective case-control, cross-sectional study included electronic health record data from 31 male patients who showed foveal cavitation on spectral-domain optical coherence tomography imaging from January 1, 2016, to June 30, 2019. Exposures Receipt of 5-ARI for at least 2 years as treatment of benign prostate hypertrophy and/or androgenic alopecia. Main Outcomes and Measures Clinical data and multimodal imaging findings and the proportion of 5-ARI users. Results Among 31 male patients with foveal cavitation, 5-ARI was used for 10 of 14 patients (71.4%) with macular abnormalities of unknown origin and for 2 of 17 patients (11.8%) with macular abnormalities of well-known specific origin (P = .001). The mean age of these 14 patients was 74.7 years (range, 60.1-88.0 years). In the 15 eyes of 10 patients who had received 5-ARI for macular abnormalities of unknown origin, mean (SD) age was 72.8 (7.5) years, mean (SD) length of time receiving 5-ARI was 72.3 (39.2) months, and mean (SD) logMAR visual acuity was 0.08 (0.10) (Snellen equivalents, 20/24 [20/25]). Optical coherence tomography imaging showed a disease spectrum ranging from tiny foveal cavitation to an impending macular hole. Of the total male patients, 80.0% (8 of 10) had no symptoms. Conclusions and Relevance The findings suggest that macular abnormalities associated with 5-ARI are characterized by cystoid abnormalities and foveal cavitation in male patients, which may progress to outer foveal defect and macular hole. These macular abnormalities associated with a male sex hormone antagonist suggested by this investigation warrant further corroboration.
Biodegradation of Polystyrene by Pseudomonas sp. Isolated from the Gut of Superworms

Large quantities of plastic waste represent a grave social issue. Various attempts have been made to solve plastic waste problems, such as methods of natural plastic degradation. Currently, polystyrene (PS) is one of the most widely used plastics in many industries; therefore, degrading PS becomes a critical global issue. In this study, we isolated Pseudomonas sp. , a strain of plastic-degrading bacteria known to survive only in the soil, from the gut of the superworms. Thus far, the degradation of PS by Pseudomonas sp. has barely been explored. We examined PS degradation using electronic microscopy, and measured changes in atomic distribution and contact angles with water droplets on the PS surface that represent a chemical change from hydrophobicity to hydrophilicity. During the process of PS degradation by Pseudomonas sp. , we examined chemical structural changes using X-ray photoelectron spectroscopy (XPS), Fourier transform-infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR) to check for the formation of C=O bonds and changes towards hydrophilicity. RT-qPCR was used to measure the gene expression level of serine hydrolase in Pseudomonas, an enzyme that mediates the plastic degradation. Our findings indicate that Pseudomonas present in the gut of the superworms participates in the degradation of plastics following ingestion. Moreover, this study also identified a candidate enzyme related to PS degradation in Pseudomonas for the first time. Thus, the findings of this study prove significance not only in presenting a novel function of Pseudomonas in the gut of superworms, but also in highlighting a potential solution for PS degradation.
Nitrogen and phosphorus cycling following prescribed burning in natural and managed Aleppo pine forests

Two prescribed burnings (downhill and uphill fires) were conducted in two stands of Aleppo pine (Pinus halepensis Mill.): a natural stand and a managed stand that was subject to thinning, pruning, and shrub removal. The concentrations of nitrogen (N) and phosphorus (P) in the pine needles and regrowth of the main shrub species, Quercus coccifera L. , and the quantities of N and P in the needle fall and in the forest floor were measured during the 6 months following the fires. The concentrations of N and P in the pine needles and leaves of Q. coccifera increased compared with the unburned control after both fires in the natural stand, where there was only a slight reduction in fuel during prescribed burnings and where there was an abundant fall of scorched needles. In contrast, the chemical composition of the foliage was unchanged after the fires in the managed stand, where there was a greater reduction in fuel, and where only small quantities of scorched needles fell. This study showed that first opening p.
Perturbations in the catecholamine metabolism and protective effect of “3-(3, 4-dimethoxy phenyl)-1-4(methoxy phenyl) prop-2-en-1-one” during ketamine-induced schizophrenia: an in vivo and in silico studies

Abstract Different kinds of secondary metabolites present in the medicinal plants play an important role to alleviate different human ailments including neurodegenerative disorders such as Parkinson’s, Alzheimer’s, epilepsy and schizophrenia etc. Recently we have isolated and characterized a novel bioactive compound viz. 3-(3,4-dimethoxy phenyl)-1-4(methoxy phenyl)prop-2-en-1-one from the methanolic extract of Celastrus paniculatus (CP) which has been widely used for the treatment of neurodegenerative diseases. The present investigation is mainly aimed to evaluate the neuroprotective potential of the above bioactive compound against ketamine-induced schizophrenia with particular reference to catecholaminergic metabolism using in vivo and in silico methods. Ketamine-induced schizophrenia caused significant elevation in biogenic amines (epinephrine, nor epinephrine, dopamine and 5-HT) and monoamine oxidase activity levels which were restored to normal during the treatment with the bioactive compound akin to the reference compound, clozapine. In addition, the compound has shown highest binding score against all the biogenic amine receptors viz. D1, D2, D3, D4 and serotonin receptor, 5-HT2A with lowest inhibition constant values than the reference compound, clozapine. The present findings suggest that modulation of CNS monoamine neurotransmitter system might partly contribute to the impairments associated with schizophrenia and the plant compound alleviates the monoaminergic abnormalities associated with the neurological dysfunction. Communicated by Ramaswamy H. Sarma
Endovascular Thrombectomy with or without Intravenous Alteplase in Acute Stroke.

BACKGROUND In acute ischemic stroke, there is uncertainty regarding the benefit and risk of administering intravenous alteplase before endovascular thrombectomy. METHODS We conducted a trial at 41 academic tertiary care centers in China to evaluate endovascular thrombectomy with or without intravenous alteplase in patients with acute ischemic stroke. Patients with acute ischemic stroke from large-vessel occlusion in the anterior circulation were randomly assigned in a 1:1 ratio to undergo endovascular thrombectomy alone (thrombectomy-alone group) or endovascular thrombectomy preceded by intravenous alteplase, at a dose of 0.9 mg per kilogram of body weight, administered within 4.5 hours after symptom onset (combination-therapy group). The primary analysis for noninferiority assessed the between-group difference in the distribution of the modified Rankin scale scores (range, 0 [no symptoms] to 6 [death]) at 90 days on the basis of a lower boundary of the 95% confidence interval of the adjusted common odds ratio equal to or larger than 0.8. We assessed various secondary outcomes, including death and reperfusion of the ischemic area. RESULTS Of 1586 patients screened, 656 were enrolled, with 327 patients assigned to the thrombectomy-alone group and 329 assigned to the combination-therapy group. Endovascular thrombectomy alone was noninferior to combined intravenous alteplase and endovascular thrombectomy with regard to the primary outcome (adjusted common odds ratio, 1.07; 95% confidence interval, 0.81 to 1.40; P = 0.04 for noninferiority) but was associated with lower percentages of patients with successful reperfusion before thrombectomy (2.4% vs. 7.0%) and overall successful reperfusion (79.4% vs. 84.5%). Mortality at 90 days was 17.7% in the thrombectomy-alone group and 18.8% in the combination-therapy group. CONCLUSIONS In Chinese patients with acute ischemic stroke from large-vessel occlusion, endovascular thrombectomy alone was noninferior with regard to functional outcome, within a 20% margin of confidence, to endovascular thrombectomy preceded by intravenous alteplase administered within 4.5 hours after symptom onset. (Funded by the Stroke Prevention Project of the National Health Commission of the People's Republic of China and the Wu Jieping Medical Foundation; DIRECT-MT ClinicalTrials.gov number, NCT03469206.).
Ampliconic Genes on the Great Ape Y Chromosomes: Rapid Evolution of Copy Number but Conservation of Expression Levels

Abstract Multicopy ampliconic gene families on the Y chromosome play an important role in spermatogenesis. Thus, studying their genetic variation in endangered great ape species is critical. We estimated the sizes (copy number) of nine Y ampliconic gene families in population samples of chimpanzee, bonobo, and orangutan with droplet digital polymerase chain reaction, combined these estimates with published data for human and gorilla, and produced genome-wide testis gene expression data for great apes. Analyzing this comprehensive data set within an evolutionary framework, we, first, found high inter- and intraspecific variation in gene family size, with larger families exhibiting higher variation as compared with smaller families, a pattern consistent with random genetic drift. Second, for four gene families, we observed significant interspecific size differences, sometimes even between sister species—chimpanzee and bonobo. Third, despite substantial variation in copy number, Y ampliconic gene families’ expression levels did not differ significantly among species, suggesting dosage regulation. Fourth, for three gene families, size was positively correlated with gene expression levels across species, suggesting that, given sufficient evolutionary time, copy number influences gene expression. Our results indicate high variability in size but conservation in gene expression levels in Y ampliconic gene families, significantly advancing our understanding of Y-chromosome evolution in great apes.