Miracle-dz/UniFAR-FTUs huggingface dataset:
Greater Maternal Weight and the Ongoing Risk of Neural Tube Defects After Folic Acid Flour Fortification

OBJECTIVE: Maternal obesity is likely a risk factor for neural tube defects (NTDs). By late 1997, it became mandatory in Canada that all refined wheat flour be fortified with folic acid. Because overweight women may consume greater quantities of refined wheat flour, we questioned whether their risk of NTD changed after flour fortification. METHODS: A retrospective population-based study was conducted between 1994 and late 2000. We included all Ontarian women who underwent antenatal maternal screening at 15 to 20 weeks of gestation. Self-declared maternal date of birth, ethnicity, current weight, and the presence of pregestational diabetes mellitus were recorded in a standardized fashion on the maternal screening requisition sheet. The presence of NTDs was systematically detected both antenatally and postnatally. The risk of open NTD was evaluated across maternal weight quartiles and deciles, and an interaction between greater maternal weight and the presence of flour fortification was tested using multiple logistic regression analysis. RESULTS: A total of 292 open NTDs were detected among 420,362 women. The adjusted odds ratio (OR) for NTD was 1.2 (95% confidence interval [CI] 1.1–1.3) per 10-kg incremental rise in maternal weight. Comparing the highest with the lowest quartile of maternal weight, the adjusted OR for NTD was 2.6 (95% CI 1.8–4.0). A similar finding was observed for the highest compared with lowest weight deciles (adjusted OR 3.3, 95% CI 1.7–6.2). The interaction between elevated maternal weight and the presence of folic acid flour fortification was of borderline significance (P = .09). Before fortification, greater maternal weight was associated with a modestly increased risk of NTD (adjusted OR 1.4, 95% CI 1.0–1.8); after flour fortification, this effect was more pronounced (adjusted OR 2.8, 95% CI 1.2–6.6). CONCLUSION: These data emphasize the higher risk of NTD associated with increased maternal weight, even after universal folic acid flour fortification. Beyond periconceptional folic acid use, consideration should be given to testing whether prepregnancy weight reduction is an independent means of preventing NTD. LEVEL OF EVIDENCE: II-2
An evidence‐based approach to medicinal plants for the treatment of sperm abnormalities in traditional Persian medicine

Infertility is defined as inability of a sexually active couple to conceive after 1 year of regular intercourse without contraception. Male factors account for 20%–50% of cases of infertility. The aim of this study was to review medicinal plants that proposed to improve sperm abnormalities in traditional Persian medicine. For this purpose, PubMed, Scopus, GoogleScholar and Cochrane library were explored for medicinal plants used in traditional Persian medicine for sperm abnormalities to obtain studies giving any evidence for their efficacy and pharmacological mechanisms related to male infertility. Data were collected for the years 1966 to March 2015. For some of them, including Chlorophytum borivilianum, Crocus sativus, Nigella sativa, Sesamum indicum, Tribulus terrestris, Mucuna pruriens and Withania somnifera, more reliable evidence was found. The mechanisms involved in the beneficial effects of medicinal plants in sperm abnormalities are antioxidant, anti‐inflammatory, anti‐oedematous and venotonic activity as well as containing precursors for sperm production and increasing blood testosterone level. Various phytochemical categories including saponins, phytosterols, carotenoids, oxygenated volatile compounds, phenolic compounds and alkaloids seem to be responsible for these beneficial effects. Further studies are recommended for obtaining more conclusive results about the efficacy and safety of the mentioned medicinal plants.
THE EVOLUTION OF F1 POSTZYGOTIC INCOMPATIBILITIES IN BIRDS

Abstract We analyzed the rate at which postzygotic incompatibilities accumulate in birds. Our purposes were to assess the role of intrinsic F1 hybrid infertility and inviability in the speciation process, and to compare rates of loss of fertility and viability between the sexes. Among our sample more than half the crosses between species in the same genus produce fertile hybrids. Complete loss of F1 hybrid fertility takes on the order of millions of years. Loss of F1 hybrid viability occurs over longer timescales than fertility: some viable hybrids have been produced between taxa that appear to have been separated for more than 55 my. There is strong support for Haldane's rule, with very few examples where the male has lower fitness than the female. However, in contrast to Drosophila, fertility of the homogametic sex in the F1 appears to be lost before viability of the heterogametic sex in the F1. We conclude that the time span of loss of intrinsic hybrid fertility and viability is often, but not always, longer than the time to speciation. Premating isolation is an important mechanism maintaining reproductive isolation in birds. In addition, other factors causing postzygotic reproductive isolation such as ecological causes of hybrid unfitness, reduced mating success of hybrids, and genetic incompatibilities in the F2s and backcrosses may often be involved in the speciation process.
Cyclophilin–CD147 interactions: a new target for anti-inflammatory therapeutics

CD147 is a widely expressed plasma membrane protein that has been implicated in a variety of physiological and pathological activities. It is best known for its ability to function as extracellular matrix metalloproteinase inducer (hence the other name for this protein, EMMPRIN), but has also been shown to regulate lymphocyte responsiveness, monocarboxylate transporter expression and spermatogenesis. These functions reflect multiple interacting partners of CD147. Among these CD147‐interacting proteins cyclophilins represent a particularly interesting class, both in terms of structural considerations and potential medical implications. CD147 has been shown to function as a signalling receptor for extracellular cyclophilins A and B and to mediate chemotactic activity of cyclophilins towards a variety of immune cells. Recent studies using in vitro and in vivo models have demonstrated a role for cyclophilin–CD147 interactions in the regulation of inflammatory responses in a number of diseases, including acute lung inflammation, rheumatoid arthritis and cardiovascular disease. Agents targeting either CD147 or cyclophilin activity showed significant anti‐inflammatory effects in experimental models, suggesting CD147–cyclophilin interactions may be a good target for new anti‐inflammatory therapeutics. Here, we review the recent literature on different aspects of cyclophilin–CD147 interactions and their role in inflammatory diseases.
Impact of fixation method on postoperative complication rates following surgical stabilization of diaphyseal tibial fractures in cats

Summary Objectives: To compare the complication rate between open reduction and internal fixation (ORIF) and external skeletal fixation (ESF) for feline diaphyseal tibial fractures. Methods: In a retrospective study spanning a 10 year period, 57 feline tibial fractures stabilized via ESF or ORIF were included for analysis and complication rates were compared between the two methods. Results: In the overall study population, 23 (40.4%) cases suffered complications (9 major, 20 minor, 6 with both major and minor). All of the major complications occurred in the ESF group. Complications were more common in cats with ESF (50.0%) while only one (7.7%) of the ORIF cases suffered complications (OR 12.0 [CI: 2.09; 228.10], p = 0.02). Use of postoperative antibiotic medications was identified as a confounder. After adjusting for confounding, stabilization using ESF remained associated with a higher risk of complications (OR = 13.71 [CI: 2.18; 274.25], p = 0.02). Cats with ESF had a longer duration of follow-up (15.6 weeks; 95% CI: 13.0; 18.3) compared to ORIF (9.5 weeks; 95% CI: 6.4; 12.7) (p = 0.003), and a higher number of revisits (mean 3.0; 95% CI: 2.4; 3.6) than the ORIF group (mean 1.6; 95% CI: 0.9; 2.3) (p = 0.002). Clinical significance: This study demonstrates a significant difference in complication rates between the methods of stabilization, with ESF resulting in a significantly higher complication rate compared to ORIF. Based on these results, it may be prudent to select ORIF for stabilization of feline tibial fractures wherever practical.
Kinematic analysis of anterior cruciate ligament reconstruction in total knee arthroplasty.

BACKGROUND This study aims to retain normal knee kinematics after knee replacement surgeries by reconstructing anterior cruciate ligament during total knee arthroplasty. METHOD We use computational simulation tools to establish four dynamic knee models, including normal knee model, posterior cruciate ligament retaining knee model, posterior cruciate ligament substituting knee model, and anterior cruciate ligament reconstructing knee model. Our proposed method utilizes magnetic resonance images to reconstruct solid bones and attachments of ligaments, and assemble femoral and tibial components according representative literatures and operational specifications. Dynamic data of axial tibial rotation and femoral translation from full-extension to 135 were measured for analyzing the motion of knee models. FINDINGS The computational simulation results show that comparing with the posterior cruciate ligament retained knee model and the posterior cruciate ligament substituted knee model, reconstructing anterior cruciate ligament improves the posterior movement of the lateral condyle, medial condyle and tibial internal rotation through a full range of flexion. The maximum posterior translations of the lateral condyle, medial condyle and tibial internal rotation of the anterior cruciate ligament reconstructed knee are 15.3 mm, 4.6 mm and 20.6 at 135 of flexion. INTERPRETATION Reconstructing anterior cruciate ligament in total knee arthroplasty has been approved to be an more efficient way of maintaining normal knee kinematics comparing to posterior cruciate ligament retained and posterior cruciate ligament substituted total knee arthroplasty.
Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy

The pathological mechanisms of diabetic retinopathy (DR), a leading cause of blindness in adults with diabetes mellitus, remain incompletely understood. Because microRNAs (miRNAs) represent effective DR therapeutic targets, we identified aberrantly expressed miRNAs associated with cellular dysfunction in early DR and detected their potential targets. We exposed human retinal endothelial cells (HRECs) and a cell line of retinal pigment epithelial (RPE) cells to high glucose (25 mmol/L, 1–7 days) to mimic DR progression and used streptozotocin-injected rats (4–8 weeks) for an in vivo diabetes model. HREC/RPE viability decreased after 24 h incubation and diminished further over 6 days, and Hoechst staining revealed hyperglycemia-induced HREC/RPE apoptosis. Although miR-124/-125b expression decreased with DR progression in vitro and in vivo, miR-135b/-199a levels decreased in retinal cells under hyperglycemia exposure, but increased in diabetic retinas. Moreover, miR-145/-146a expression decreased gradually in high-glucose-treated HRECs, but increased in hyperglycemia-exposed RPE cells and in diabetic rats. Our findings suggested that aberrant miRNA expression could be involved in hyperglycemia-induced retinal-cell dysfunction, and the identified miRNAs might vary in different retinal layers, with expression changes associated with DR development. Therefore, miRNA modulation and the targeting of miRNA effects on transcription factors could represent novel and effective DR-treatment strategies.
Transition outcomes for young adults with disabilities.

PURPOSE Transition to adulthood can be very challenging for children with special health care needs (CSHCN) especially for those with disabilities who experience functional limitations in activities at home, in school, and in the community. The study examined the transition outcomes in areas of health, education, and independent living for young adult with special health care needs (YASHCN) with disabilities. METHOD The study is a secondary data analysis of the 2007 Survey of Adult Transition and Health (SATH). Multivariate logistic regression analysis assessed the association between having disabilities and the transition outcomes. RESULTS Overall, YASHCN with disabilities reported favorable health related transition outcomes with improved access to primary care, care coordination, and physician engagement in transition discussions and connection to mentors. Furthermore, YASCHN with disabilities had higher odds of receiving Medicaid or other insurance for low income or disabilities as an adult (AOR=5.26, 95% CI=3.74, 7.04). However, they were less likely to report having control over personal finances, making friends, and obtaining a high school diploma. CONCLUSION The findings suggest that YASHCN with disabilities may be among the small proportion of CSHCNs who had a positive transition to adult health care services. However, transition outcomes related to independent living still need more improvements.
Basal and Stimulated Protein S-Nitrosylation in Multiple Cell Types and Tissues*

There is substantial evidence that proteinS-nitrosylation provides a significant route through which nitric oxide (NO)-derived bioactivity is conveyed. However, most examples of S-nitrosylation have been characterized on the basis of analysis in vitro, and relatively little progress has been made in assessing the participant forms of nitric-oxide synthase (NOS) or the dynamics of protein S-nitrosylationin situ. Here we utilize antibodies specific for the nitrosothiol (SNO) moiety to provide an immunohistochemical demonstration that protein S-nitrosylation is coupled to the activity of each of the major forms of NOS. In cultured endothelial cells, SNO-protein immunoreactivity increases in response to Ca2+-stimulated endothelial NOS (eNOS) activity, and in aortic rings, endothelium-derived and eNOS-mediated relaxation (EDRF) is coupled to increased protein S-nitrosylation in both endothelial and associated smooth muscle cells. In cultured macrophages, SNO-protein levels increase upon cytokine induction of induced NOS (iNOS), and in PC12 cells, increased proteinS-nitrosylation is linked to nerve growth factor induction of neuronal NOS (nNOS). In addition, we describe developmental and pathophysiological increases in SNO-protein immunoreactivity within human lung. These results, which demonstrate Ca2+, neurohumoral, growth factor, cytokine, and developmental regulation of protein S-nitrosylation that is coupled to NOS expression and activity, provide unique evidence for the proposition that this ubiquitous NO-derived post-translational protein modification serves as a major effector of NO-related bioactivity.
RNA Interference Screen Reveals an Essential Role of Nedd4–2 in Dopamine Transporter Ubiquitination and Endocytosis

The function of the dopamine transporter (DAT) to terminate dopamine neurotransmission is regulated by endocytic trafficking of DAT. To elucidate the mechanisms of DAT endocytosis, we generated a fully functional mutant of the human DAT in which a hemagglutinin epitope (HA) was incorporated into the second extracellular loop. The endocytosis assay, based on the uptake of an HA antibody, was designed to study constitutive- and protein kinase C (PKC)-dependent internalization of HA-DAT expressed in non-neuronal cells and rat dopaminergic neurons. Large-scale RNA interference analysis of PKC-dependent endocytosis of HA-DAT revealed the essential and specific role of an E3 ubiquitin ligase, Nedd4–2 (neural precursor cell expressed, developmentally downregulated 4–2), as well as the involvement of adaptor proteins present in clathrin-coated pits, such as epsin, Eps15 (epidermal growth factor pathway substrate clone 15), and Eps15R (Eps15-related protein). Depletion of Nedd4–2 resulted in a dramatic reduction of PKC-dependent ubiquitination of DAT. Endogenous Nedd4–2, epsin, and Eps15 were coimmunoprecipitated with heterologously expressed human HA-DAT and endogenous DAT isolated from rat striatum. A new mechanistic model of DAT endocytosis is proposed whereby the PKC-induced ubiquitination of DAT mediated by Nedd4–2 leads to interaction of DAT with adaptor proteins in coated pits and acceleration of DAT endocytosis.
Basic Fibroblast Growth Factor Modulates Density of Blood Vessels and Preserves Tight Junctions in Organotypic Cortical Cultures of Mice: A New In Vitro Model of the Blood–Brain Barrier

This study was performed to examine the maintenance of blood vessels in vitro in cortical organotypic slice cultures of mice with special emphasis on basic fibroblast growth factor (FGF-2), which is known to promote angiogenesis and to preserve the integrity of the blood–brain barrier. Slices of neonatal day 3 or 4 mouse brain were maintained for 3, 7, or 10 d in vitro (DIV) under standard culture conditions or in the presence of FGF-2. Immunohistochemistry for factor VIII-related antigen or laminin revealed a relative low number of blood vessels under standard conditions. In contrast, moderate FGF-2 concentrations increased the number of vessels: with 0.5 ng/ml FGF-2 it was 1.4-fold higher after DIV 3 or 1.5-fold after DIV 7 compared with controls; with 5 ng/ml it was almost doubled in both cases. With an excess of 50 ng/ml, FGF-2 vessels were reduced after DIV 3 or similar to controls after DIV 7. FGF receptor 1 was preferentially found on endothelial cells; its immunolabeling was reduced in the presence of the ligand. Cell death detected by an ethidium bromide analog or the apoptosis marker caspase-3 was barely detectable during the 10 d culture period. Immunolabeling of the tight junction proteins ZO-1 (zonula occludens protein 1), occludin, claudin-5, and claudin-3 revealed evidence for structural integrity of the blood–brain barrier in the presence of moderate FGF-2 concentrations. In conclusion, FGF-2 maintains blood vessels in vitro and preserves the composition of the tight junction. Hence, we propose FGF-2-treated organotypic cortical slices as a new tool for mechanistic studies of the blood–brain barrier.
Has implementing an ecolabel increased sustainable tourism in Barcelona

The aim of this study is to find out if by implementing an ecolabel in destinations, applying an SDM model, tourism sustainability increases. In order to this, three measures have been put into practice in Barcelona: (1) a focus group interview to key stakeholders (DMOs) see their implication with the certification project; (2) the implementations of two different actions relevant for the study: (a) to confront and pair up the certification body´s set of criteria with the Global Sustainable Tourism Council (GSTC) ones, (b) check if the certification adapts to Font´s study about ecolabels; and finally, (3) a discussion to find out if the media and local citizens felt in a city touristic ally sustainable. The findings show that Barcelona tourism board and other stakeholders were concerned about tourism sustainability. Besides, ecolabel can be believed to be trustworthy and reliable by adapting it to Font´s study (2001) and by confronting it with GSTC destination criteria. However, local population seems not to be satisfied at all with how the city is getting so many tourism. They seems not to understand labels, but they understand the negative impacts from tourism in their way of life. The limitation to this study is that just the Barcelona case is analyzed. The contribution is that it is actually putting into practice well known concepts that have usually been studied in theoretical ways.
V-BLAST transmission scheme based on mobile robot in WSN

Aiming at the requirements of high-speed data transmission and energy consumption in the case of emergency relief and real-time detection, a new mobile robot & V-BLAST based cooperative MIMO transmission scheme and a new efficient ML detection algorithm are proposed in this paper. Mobility of robot can reduce the communication distance between the sensor nodes, MIMO-based WSN can use its diversity gain to overcome fading effects and can also use its multiplexing gain to increase the data transmission rate. Combined with traditional decoding OSIC algorithm, the vectors need to be detected in Low-complexity approximate maximum likelihood algorithm are reduced. So, the computational complexity of the proposed algorithm is decreased greatly. Simulation results show that the proposed scheme effectively reduces network energy consumption, and the low-complexity maximum likelihood detection algorithm further improve energy-efficiency.
Victorian Voting: Party Orientation and Class Alignment Revisited

Much of what we know about the alignment of voters with parties comes from mass surveys of the electorate in the postwar period or from aggregate electoral data. Using individual elector level panel data from the 19th century UK poll books, we reassess the development of a party-centred electorate in the United Kingdom. We show that (i) the electorate was party-centred by the time of the extension of the franchise in 1867; (ii) a decline in candidate-centred voting is largely attributable to changes in the behaviour of the working class and (iii) the enfranchised working class aligned with the Liberal left. This early alignment of the working class with the left cannot entirely be explained by a decrease in vote buying. The evidence suggests instead that the alignment was based on the programmatic appeal of the Liberals. We argue that these facts can plausibly explain the subsequent development of the party system.
Medication use among pediatric patients with chronic musculoskeletal pain syndromes at initial pain clinic evaluation.

AIM To characterize medication use by adolescents with chronic musculoskeletal pain syndromes before an initial multidisciplinary clinic visit. PATIENTS & METHODS A cross-sectional sample of 120 adolescents and parents reported on standardized assessment measures, with medication use data extracted from the medical chart and categorized. RESULTS On average, 3.2 medications were reported; 70% used more than one pain-specific medication including opioids (17%), nonopioids (31%), psychotropics/neuropathics (45%) and other medications (13%). Adolescents with complex regional pain syndrome consistently reported greatest use of opioid, psychotropic/neuropathic and other pain medications. A regression model explained 17% of the variance in pain medication use. Nonpain medication use and disability contributed unique variance - pain duration and intensity did not. CONCLUSION Greater attention to factors contributing to prescriptive practices, medication use and long-term outcomes is warranted.