Miracle-dz/UniFAR-FTUs huggingface dataset:
Modelling of axonal B fields for Direct Neuronal Detection (DND) using MRI

Medical imaging has seen great advances over the last twenty years since the introduction of Magnetic Resonance Imaging (MRI). MRI has proved to be a very powerful technique providing the opportunity to observe very detailed body images which has taken the field of body and neuro-imaging to a new level. In addition MRI is widely used for functional imaging with a high spatial resolution. Functional imaging of the brain for the purpose of medical diagnosis and research has relied previously on the hemodynamic response based technique BOLD (Blood Oxygen Level Dependent) fMRI (functional Magnetic Resonance Imaging). Although this is a very powerful and informative imaging method the temporal resolution can be improved by exploiting other information that might be present when imaging the brain. The hypothesis tested here is that the weak neuronal magnetic fields that result from the local neuronal currents cause scanner field perturbations strong enough to appear as contrast on MR images. Such Direct Neuronal Detection (DND) of neuronal currents would allow real time imaging of the electrical activity of detailed regions of the brain, potentially leading to a better understanding of brain functionality.
Immunological changes in young and old adults during brief laboratory stress.

&NA; Few data are available on the response of the human immune system to acute psychological stressors under controlled laboratory conditions. Young female subjects (21–41 years) showed increases in natural killer (NK) cell activity, and in the numbers of circulating CD8 suppressor/cytotoxic T cells, and natural killer lymphocytes following a brief (12 minute) stressful mental arithmetic examination. Older female subjects (65–85 years) failed to show the stress‐related increase in NK activity. The psychological stress did lead to increases in the numbers of circulating CD8 suppressor/cytotoxic T cells and NK lymphocytes in old subjects to a similar degree as that seen in the young group. No changes in the numbers of helper/inducer T cells (CD4), total T cells (CD3), or B cells (CD20) were found following the stressor for either group. Cardiovascular, catecholamine, and subjective stress responses were similar for the two age groups. These results demonstrate that brief psychological stress is associated with some rapid immune cell changes, including release of CD8 suppressor/cytotoxic T cells and NK cells into circulation, and in young subjects, increases in NK activity. The absence of an NK activity increase in the older subjects indicates that NK cell mobilization and cell lysis induced by NK cells may be differentially affected by stress. The results also suggest the possibility of an age‐related deficit in the up‐regulation of NK activity under some environmental demands.
Embedded Countermovements: The Forging of Protected Areas and Native Communities in the Peruvian Amazon

ABSTRACT With the progressive establishment of protected areas for conservation and communal indigenous land, market economy has been regulated in the Peruvian Amazon. Interlinked networks and institutions situate the agency of institutional entrepreneurs championing forest protection and indigenous peoples’ rights over territory and resources. This paper shows how a critical integration of new institutionalisms in the social sciences provides a suitable theory frame to study the process of protectionist institutional change as envisioned by Karl Polanyi. The cumulative result of socially-embedded state activism –that is, regulatory activism that is embedded in society– is an institutional regime that strengthens the resources and capabilities of conservationist groups and Amazonian indigenous peoples’ organisations as collective constructs while facing the social forces of commodification.
Suicidal behavior, smoking, and familial vulnerability.

INTRODUCTION Smoking is a well-established correlate of suicidal behavior. It is not known if familial risk factors contribute to this association. METHODS Data were obtained via semistructured interviews with 1,107 twin fathers, 1,919 offspring between ages 12-32 years, and 1,023 mothers. Familial vulnerability to nicotine dependence and suicidal behavior was modeled via father and maternal self-report of these behaviors. Multinomial logistic regression models were computed with and without familial risk factors to estimate the association between offspring ever smoking, regular smoking, nicotine dependence, and a 4-level offspring suicide variable: (a) none, (b) ideation, (c) ideation + plan, and (d) ideation + plan + attempt or ideation + attempt. All models were stratified by gender and adjusted for sociodemographics, familial risk factors including parental suicidal behavior, nicotine dependence, and conduct disorder, and offspring conduct disorder, depression, alcohol abuse/dependence, and illicit drug abuse/dependence. RESULTS After adjusting for covariates and familial risk factors, ever smoking was not significantly associated with suicidal behavior in males and females. In males, regular smoking was associated with ideation + plan (odds ratio [OR] = 5.47; 95% CI: 1.05-28.60), and in females, regular smoking was associated with ideation + plan + attempt or ideation + attempt. In both genders, nicotine-dependent smoking was associated with ideation + plan + attempt or ideation + attempt (males: OR = 6.59; 95% CI: 1.91-22.70, females: OR = 3.37; 95% CI: 1.25-9.04). Comparison of models with and without familial risk factors indicated that there is no mediation of smoking status and suicidal behavior by familial risk. CONCLUSIONS Smoking and nicotine dependence are correlated with suicidal behaviour. Contributions from familial risk factors did not significantly alter this association.
Within‐Industry Agglomeration of Occupations: Evidence from Census Microdata

This study uses worker‐level data on industry, occupation, and place of work to explore differences in the spatial properties of production, administrative, and R&D occupation groups within industries. To measure differences, we calculate location quotients at the local labor market level and the Duranton and Overman (2005) agglomeration index for each group. We find appreciable differences in the spatial distribution of occupation groups within most manufacturing industries, with R&D occupations consistently exhibiting the highest degree of spatial concentration. Our results are consistent with the core theoretical and empirical results in the agglomeration literature.
Prevalence and Clinical Correlates of Residual Depressive Symptoms in Bipolar II Disorder

Background: Most patients with unipolar and bipolar I disorder have residual symptoms, despite successful treatment. The appraisal of subsyndromal symptomatology has important implications for pathophysiological models of disease and relapse prevention. Residual symptoms in bipolar II disorder were studied insufficiently. The study of residual symptoms in bipolar II disorder is important, because many depressed outpatients may suffer from it and because bipolar II disorder may be distinct from type I. The study aims were to assess the prevalence and clinical correlates of persistent residual depressive symptoms in bipolar II disorder. Methods: 138 consecutive patients with bipolar II disorder and 83 unipolar disorder outpatients, presenting for major depressive episode treatment in private practice, were interviewed with the Structured Clinical Interview for DSM-IV Axis I Disorders – Clinician’s Version. Study variables were persistent (more than 2 years) residual depressive symptoms, age, gender, age at onset, illness duration, recurrences, axis I comorbidity, severity, psychotic, melancholic and atypical features. Results: The prevalence of residual depressive symptoms was 44.9% in bipolar II disorder and 43.3% in unipolar disorder. Residual depressive symptoms in bipolar II and unipolar disorders were significantly and positively associated with illness duration and recurrences. Conclusions: Persistent residual depressive symptoms were common in bipolar II disorder. Residual unipolar and bipolar II depressive symptoms were related to duration of illness and number of recurrences. Reducing these variables could reduce and prevent residual symptoms. A mechanism of kindling (more mood episodes leading to worse outcome) could be that of leaving a larger and larger amount of residual symptoms after the acute episode has subsided.
Large-Scale Analysis of Genes that Alter Sensitivity to the Anticancer Drug Tirapazamine in Saccharomyces cerevisiaes⃞

Tirapazamine (TPZ) is an anticancer drug that targets topoisomerase II. TPZ is preferentially active under hypoxic conditions. The drug itself is not harmful to cells; rather, it is reduced to a toxic radical species by an NADPH cytochrome P450 oxidoreductase. Under aerobic conditions, the toxic compound reacts with oxygen to revert back to TPZ and a much less toxic radical species. We have used yeast (Saccharomyces cerevisiae) as a model to better understand the mechanism of action of TPZ. Overexpression of NCP1, encoding the yeast ortholog of the human P450 oxidoreductase, results in greatly increased sensitivity to TPZ. Likewise, overexpression of TOP2 (encoding topoisomerase II) leads to hypersensitivity to TPZ, suggesting that topoisomerase II is also a target of TPZ in yeast. Thus, our data show that yeast mimics human cells in terms of TPZ sensitivity. We have performed robot-aided screens for altered sensitivity to TPZ using a collection of approximately 4600 haploid yeast deletion strains. We have identified 117 and 73 genes whose deletion results in increased or decreased resistance to TPZ, respectively. For example, cells lacking various DNA repair genes are hypersensitive to TPZ. In contrast, deletion of genes encoding some amino acid permeases results in cells that are resistant to TPZ. This suggests that permeases may be involved in intracellular uptake of TPZ. Our discoveries in yeast may lead to a better understanding of TPZ biology in humans.
Metabolic correlates with obstructive sleep apnea in obese subjects.

OBJECTIVE To examine links between obstructive sleep apnea (OSA), insulin resistance, and dyslipidemia. STUDY DESIGN Obese (body mass index [BMI] >95th percentile for age and gender) children who snored (n = 62, 46 males, age 10.89 [5-16 years] underwent polysomnography and metabolic studies. RESULTS Respiratory disturbance index (RDI) was 9.23 (0-95), with 23 children (39%) recommended for treatment. Fasting insulin levels were 154.6 pmol/L +/- 79.8 (52-486), and fasting glucose levels were high in 7 children (11%). Fasting insulin levels correlated with sleep variables, including log transformed RDI (log(10)RDI) (P =.01), desaturation events (P =.05), arousal index (P =.01), and sleep-time with oxygen saturation in arterial blood <90% (P =.03) (adjusted r (2) = 0.21, F = 3.9, P =. 005), but not with age, or BMI Z score. Log(10)RDI correlated with fasting insulin (P =.001) and BMI Z score (P =.03) (adjusted r (2) = 0.12, F = 3.9, P =. 005), but not age or other metabolic variables. The correlation between log(10)RDI and fasting insulin persisted in models combining sleep and metabolic variables: log(10)RDI, adjusted r (2) = 0.75, F = 35.2, P <. 001, and for fasting insulin, adjusted r (2) = 0.42, F = 6.1, and P <. 001. CONCLUSIONS The severity of OSA (log(10)RDI) correlated with fasting insulin levels, independent of BMI. Insulin levels may be further elevated as a consequence of OSA in obese children.
Short-term tracheal occlusion in fetal lambs with diaphragmatic hernia improves lung function, even in the absence of lung growth.

BACKGROUND/PURPOSE Prolonged tracheal occlusion (TO) accelerates lung growth but impairs surfactant production. Short-term TO results in less lung growth but preserves type II cell function. The authors studied the effects of short-term TO on lung physiology in diaphragmatic hernia. METHODS Diaphragmatic hernia was created in 9 fetal lambs at 90 to 95 days. Five were left uncorrected (CDH), 4 underwent 2-week TO (108 to 122 days; CDH + TO). Five unoperated lambs served as controls. Near-term (136 days) fetuses were ventilated for 90 to 150 minutes. Pulmonary arterial pressure, postductal blood gases, quasistatic compliance, total lung capacity (TLC), and lung weight to body weight (LW/BW) were measured. RESULTS There was an overall survival rate of 89% at full term. Short-term occlusion did not induce lung growth (TLC and LW/BW, 6.07 +/- 2.92 mL/kg and 0.022 +/- 0.008 in CDH, 4.86 mL/kg and 0.019 +/- 0.005 in CDH + TO, 10.81 +/- 3.55 mL/kg and 0.036 +/- 0.006 in controls, respectively). However, pulmonary hypertension in CDH (47.4 +/- 12.32/35.8 +/- 12.19 torr) was corrected by short-term occlusion (20.2 +/- 4.0/16.0 +/- 4.8 torr in CDH + TO, P< .05, single-factor analysis of variance [ANOVA]; similar to control). Best pO2 and pCO2 improved after occlusion (CDH, 48.6 +/- 6.7 torr and 107.1 +/- 34.3 torr, respectively; CDH + TO, 101.5 +/- 16.3 torr and 81.9 +/- 2.4 torr; control, 291.4 +/- 4.7 torr and 37.7 +/- 17.3), as did oxygenation index (P < .05, CDH vCDH + TO; CDH, 97.2 +/- 23.0; CDH + TO, 28.7 +/- 3.1; control, 5.6 +/- 0.6). CONCLUSIONS Short-term TO corrects pulmonary hypertension and improves gas exchange in fetal lambs with diaphragmatic hernia despite failure to produce accelerated lung growth. Inducing lung maturation and correcting the physiological derangement in diaphragmatic hernia may be more important than achieving lung growth alone.
Inflation Dynamics In A Small Developing Economy: An Empirical Analysis for Ghana

ABSTRACT:Monetary policy over the data period is aimed at sustaining the growth in the money supply achieved through a tight monetary policy stance, thereby ensuring price and exchange rate stability. This paper aims to empirically examine the share of exchange rate pass-through and local monetary targets in explaining inflation dynamics in Ghana. Annual data is obtained from International Financial Statistics, Organization for Economic Cooperation and Development, and Bank of Ghana’s Annual Reports. The aggregate time series used in the estimation are money (broad money supply, M2, defined as currency plus demand, time and savings deposits held by the public), real income per capita, and the real effective exchange rate. The UK consumer Price index is used as a proxy for the foreign price level since the UK has been Ghana’s largest trading partner over much of the data period. The Treasury bill rate is used as the domestic nominal rate of interest. The Consumer Price Index measures the domestic price level. Using Pesaran, Shin and Smith’s bounds testing approach to cointegration and an error correction model, the paper finds empirical evidence for both short and long run inflationary consequences from changes in monetary factors. The results indicate that changes in money supply, real effective exchange rate, nominal interest rate, real income per capita and foreign prices have a significant influence on the behavior and path of inflation in the long run. However, the short-run dynamics based on an error correction model shows that money supply has no significant impact on the rate of inflation. While real effective exchange rate and nominal interest rate have significant effects, the main driving force behind domestic inflation in the short-run appears to be import prices and real income per capita. The findings highlight the need for an alternative operational target such as the monetary conditions index or an explicit inflation-targeting framework to improve policy credibility.
How to Draft ‘Good’ Minutes of Monetary Policy Decisions

The minutes of monetary policy decisions at central banks vary substantially. A common norm does not exist. To ensure accountability and guide policy expectations, the minutes should satisfy some basic requirements. In search of criteria for ‘good’ minutes, it is relevant to look at how supreme courts write their decisions. Four criteria for ‘good’ minutes are proposed. The minutes of six central banks are assessed against the proposed criteria. In spite of the substantial variation in format, their minutes largely satisfy the criteria. The central banks' minutes seem to reflect their individual communication histories. The central banks could learn from each other, but also from the supreme courts, especially in terms of how they organise the writing of the minority view.
Chemosensory regulation of developmental gene expression in Myxococcus xanthus

The δ-proteobacterium Myxococcus xanthus coordinates its motility during aggregation and fruiting body formation. While searching for chemotaxis genes in M. xanthus, we identified a third chemotaxis-like gene cluster, the che3 cluster, encoding homologs to two methyl-accepting chemotaxis proteins (MCPs), a CheW, a hybrid CheA, a CheB, a CheR, but no CheY. Mutations in mcp3A, mcp3B, and cheA3 did not show obvious defects in motility or chemotaxis but did affect the timing of entry into development. Mutations in these genes caused early aggregation of starving cells, even at low cell densities. Furthermore, these mutants showed pronounced overexpression of the developmentally regulated Tn5lac fusions Ω4403, Ω4411, and Ω4521 as well as overexpression of mbhA and tps, markers for peripheral rods and aggregating cells, respectively. Divergently transcribed from the che3 promoter region is another gene, crdA (chemosensory regulator of development), predicted to encode a transcriptional activator of σ54-dependent promoters. To test the hypothesis that CrdA functions as the cognate response regulator for the histidine kinase CheA3, CrdA and CheA3 were assayed and found to interact strongly in the yeast two-hybrid system. Mutant analysis showed that crdA cells were delayed in development (12–24 h) and delayed in MbhA production relative to the wild type. An mcp3BcrdA double mutant displayed the crdA phenotype, indicating that crdA is epistatic to mcp3B. We conclude that the Che3 chemotaxis-like system functions to control developmental gene expression by regulating a σ54 transcriptional activator, CrdA.
Predicting the Development of Tuberculosis with the Tuberculin Skin Test and QuantiFERON Testing.

RATIONALE The identification of patients with latent tuberculosis infection, who are at higher risk to develop active disease, is an important component of disease control. OBJECTIVES We aim to compare the usefulness of the QuantiFERON-TB Gold in-tube assay and the tuberculin skin test to predict the development of active tuberculosis during follow-up, using positive and negative predictive values, positive likelihood ratios, and stratified level of risk. METHODS The study included contacts of tuberculosis cases diagnosed between 2007 and 2009. All contacts included were from the first circle of exposure. Tuberculin skin test and QuantiFERON test were performed and a chest radiograph was obtained during the contact's study. MEASUREMENTS AND MAIN RESULTS A total of 1,335 contacts were followed up for 4 years: a smear-positive index case was identified for 937 contacts, of whom 15 developed active tuberculosis and had initially presented with positive tuberculin skin test/QuantiFERON results, a normal chest radiograph, and no symptoms. The positive predictive value was 4% for QuantiFERON and 2% for the tuberculin skin test (when ≥5 mm). The probability of developing active disease was 2.36 times higher with a positive QuantiFERON, and 1.3 times higher with a positive tuberculin skin test. The positive predictive value was 17%, and the positive likelihood ratio was 7.53 for untreated contacts with a positive QuantiFERON. Stratifying according to initial QuantiFERON results showed a 6.36 times higher risk of developing active tuberculosis for patients with a QuantiFERON result greater than or equal to 10 IU/ml. Among bacillus Calmette-Guérin-vaccinated patients, a tuberculin skin test induration greater than or equal to 15 mm correlated better with a positive QuantiFERON. CONCLUSIONS QuantiFERON results were more accurate than tuberculin skin test results in predicting tuberculosis. Although all contacts with QuantiFERON-positive results are at risk of developing tuberculosis, those with a tuberculin skin test induration greater than or equal to 15 mm and QuantiFERON greater than or equal to 10 IU/ml are at highest risk. This has important implications in the clinical management of tuberculosis contacts.
Comparative Evaluation of Anti-HER2 Affibody Molecules Labeled with 64Cu Using NOTA and NODAGA

Imaging using affibody molecules enables discrimination between breast cancer metastases with high and low expression of HER2, making appropriate therapy selection possible. This study aimed to evaluate if the longer half-life of 64Cu (T1/2 = 12.7 h) would make 64Cu a superior nuclide compared to 68Ga for PET imaging of HER2 expression using affibody molecules. The synthetic ZHER2:S1 affibody molecule was conjugated with the chelators NOTA or NODAGA and labeled with 64Cu. The tumor-targeting properties of 64Cu-NOTA-ZHER2:S1 and 64Cu-NODAGA-ZHER2:S1 were evaluated and compared with the targeting properties of 68Ga-NODAGA-ZHER2:S1 in mice. Both 64Cu-NOTA-ZHER2:S1 and 64Cu-NODAGA-ZHER2:S1 demonstrated specific targeting of HER2-expressing xenografts. At 2 h after injection of 64Cu-NOTA-ZHER2:S1, 64Cu-NODAGA-ZHER2:S1, and 68Ga-NODAGA-ZHER2:S1, tumor uptakes did not differ significantly. Renal uptake of 64Cu-labeled conjugates was dramatically reduced at 6 and 24 h after injection. Notably, radioactivity uptake concomitantly increased in blood, lung, liver, spleen, and intestines, which resulted in decreased tumor-to-organ ratios compared to 2 h postinjection. Organ uptake was lower for 64Cu-NODAGA-ZHER2:S1. The most probable explanation for this biodistribution pattern was the release and redistribution of renal radiometabolites. In conclusion, monoamide derivatives of NOTA and NODAGA may be suboptimal chelators for radiocopper labeling of anti-HER2 affibody molecules and, possibly, other scaffold proteins with high renal uptake.
Sleep disturbance is common among servicemembers and veterans of Operations Enduring Freedom and Iraqi Freedom.

Sleep routines that develop as an adaptation or reaction to deployment can persist upon return stateside. Sleep problems intensify and are intensified by psychiatric distress. This research presents the findings of a comprehensive survey of sleep impairment in relation to demographic data, military history, combat exposure, and mental illness symptoms among a general sample of 375 servicemembers and veterans of Operations Enduring Freedom and Iraqi Freedom (OEF/OIF) at a wide range of times postdeployment. Sleep impairment was assessed with the Pittsburgh Sleep Quality Index (PSQI) and the Addendum for PTSD. Posttraumatic stress disorder (PTSD), depression, and anxiety symptoms were evaluated, with the PTSD Checklist-Military, the Patient Health Questionnaire-9 and the Generalized Anxiety Disorder-7. Sleep problems were common across the sample, with 45.4% of participants reporting sleep onset greater than 30 minutes, 21.4% typically achieving less than 4.5 hours of total sleep time, and 56% reporting being awake in bed more than 15% of the night. Global PSQI scores classified 89% of the sample as "poor sleepers. " Sleep problems were more severe among servicemembers with less education, from lower ranks (E1-E3), with greater combat exposure, and greater depression, anxiety, and PTSD symptoms. These findings suggest the need for routine screening of sleep problems among veterans and increased professional training in interventions for insomnia and nightmares. For individuals experiencing sleep problems with concurrent psychiatric symptoms, addressing sleep concerns may be one less-stigmatizing way to transition servicemembers and veterans into needed mental health services.