Miracle-dz/UniFAR-FTUs huggingface dataset:
Phytochemical Profile and Antioxidant Activities of Coleus amboinicus Lour. Cultivated in Indonesia and Poland

Coleus amboinicus Lour. , Lamiaceae, is a perennial herb that is native to Indonesia and also cultivated in Africa, Asia and Australia. The major phytochemicals responsible for its bioactivity are rosmarinic acid (RA) and its analogues, flavonoids and abietane diterpenoids. The possibility of cultivation in a colder climate would extend the use of this herb and provide new opportunities to herb growers and livestock farmers. Our study to compare feed value and phytochemical composition of C. amboinicus plants cultivated in its original region, Indonesia, and in Poland. The crude protein content was significantly higher in plants cultivated in Indonesia compared to those cultivated in Poland—21% and 13% of dry matter, respectively. The higher ADF contents were detected in C. amboinicus cultivated in Indonesia, 38–41%, in comparison to 34% in plants cultivated in Poland. The phytochemical composition was also significantly influenced by the cultivation location. Polish samples were higher in polyphenols (RA and its analogues), and also had 1.5–2-fold higher antioxidant potential, as measured by DPPH scavenging, phosphomolybdenum reduction and Fenton reaction driven lipid peroxidation. The Indonesian samples contained more diterpenoid compounds, such as dihydroxyroyleanone, and the sum of terpenoids was ca. 10 times higher than in samples from Poland (15.59–23.64 vs. 1.87 µg/g of extracts). In conclusion, C. amboinicus is suitable for cultivation in non-optimal climatic conditions but some nutritional properties and bioactivity are significantly affected.
Perception and Knowledge of Patients from Different Regions in the Kingdom of Saudi Arabia towards Oral Hygiene and Oral Hygiene Aids

Aim: The present study aims to evaluate the perception and awareness of interdental aids in different regions of Saudi Arabia. Methods: A cross-sectional questionnaire-based study was conducted, in order to evaluate the perception and knowledge of patients towards oral hygiene products among the population of the Kingdom of Saudi Arabia. In total, 812 out of 1124 participants responded and completed the survey (response rate 72.2%). The data were collected using a self-administered structured questionnaire in English and Arabic. All statistical analyses were carried out using the SPSS 20 software. p < 0.05 was used to indicate statistical significance. Results: A total of 812 participants responded, of which 486 participants (60%) declared using a toothpaste and toothbrush for cleaning their teeth. The cohort consisted of 274 (34%) females who brushed twice daily, while 96 (33%) males brushed their teeth once a day and 18% of the participants did not even brush once a day. The results indicated that 332 (64%) female participants and 174 (60%) male participants had perception and knowledge of the use of dental floss or any other device to clean between their teeth, while 174 (48.50%) male participants and 174 (49.10%) female participants cleaned their tongue with the same brush, rather than using a tongue scrubber or any other aids. Conclusion: The total awareness of interdental aids in Saudi Arabia is unsatisfactory, as demonstrated by the participants not being conscious or informed about the maintenance of their oral health. A majority of participants did not report adopting basic techniques, such as tongue brushing. This study reveals that no interdental aids were used by 16% of the participants. Thus, it is crucial to develop an effective educational program which emphasizes oral healthcare in this population.
A Novel V2V Cooperative Collision Warning System Using UWB/DR for Intelligent Vehicles

The collision warning system (CWS) plays an essential role in vehicle active safety. However, traditional distance-measuring solutions, e.g., millimeter-wave radars, ultrasonic radars, and lidars, fail to reflect vehicles’ relative attitude and motion trends. In this paper, we proposed a vehicle-to-vehicle (V2V) cooperative collision warning system (CCWS) consisting of an ultra-wideband (UWB) relative positioning/directing module and a dead reckoning (DR) module with wheel-speed sensors. Each vehicle has four UWB modules on the body corners and two wheel-speed sensors on the rear wheels in the presented configuration. An over-constrained localization method is proposed to calculate the relative position and orientation with the UWB data more accurately. Vehicle velocities and yaw rates are measured by wheel-speed sensors. An extended Kalman filter (EKF) is applied based on the relative kinematic model to combine the UWB and DR data. Finally, the time to collision (TTC) is estimated based on the predicted vehicle collision position. Furthermore, through UWB signals, vehicles can simultaneously communicate with each other and share information, e.g., velocity, yaw rate, which brings the potential for enhanced real-time performance. Simulation and experimental results show that the proposed method significantly improves the positioning, directing, and velocity estimating accuracy, and the proposed system can efficiently provide collision warning.
Development of a 2-D Array Ultrasonic Transducer for 3-D Imaging of Objects Immersed in Water

Most works that address 2-D array ultrasonic transducers for underwater applications are about the geometry aspects of the array and beamforming techniques to make 3-D images. They look for techniques to reduce the number of elements from wide apertures, maintaining the side lobes and the grating lobes at acceptable levels, but not many details about the materials and fabrication processes are described. To overcome these gaps, this paper presents in detail the development of a 2-D array ultrasonic transducer prototype that can individually emit and receive ultrasonic pulses to make 3-D images of immersed reflectors within a volume of interest (VOI). It consists of a 4 × 4 matrix ultrasonic transducer with a central frequency of 480 kHz. Each element is a 5 mm sided square cut into a 1–3 piezocomposite. The center-to-center distance of two contiguous elements (pitch) was chosen to be greater than half wavelength, to increase the amplitude of emission and reception of signals with larger elements. Artifacts generated by grating lobes were avoided by restricting the field of view in the azimuth and elevation directions within 40° × 40° and applying the sign coherence factor (SCF) filter. Two types of backing layer materials were tested, one with air and another made of epoxy resin, on the transducers called T1 and T2, respectively. The pulse echoes measured with T1 had 2.6 dB higher amplitude than those measured with T2, and the bandwidths were 54% and 50% @ −6 dB, respectively, exciting the element with a single rectangular negative pulse. The 3-D images obtained with full matrix capture (FMC) data sets acquired of objects from 0.2 to 1.15 m motivate the development of a 2-D array transducer with more elements, to increase the angular resolution and the range.
Influence of Tumor Microenvironment and Fibroblast Population Plasticity on Melanoma Growth, Therapy Resistance and Immunoescape

Cutaneous melanoma (CM) tissue represents a network constituted by cancer cells and tumor microenvironment (TME). A key feature of CM is the high structural and cellular plasticity of TME, allowing its evolution with disease and adaptation to cancer cell and environmental alterations. In particular, during melanoma development and progression each component of TME by interacting with each other and with cancer cells is subjected to dramatic structural and cellular modifications. These alterations affect extracellular matrix (ECM) remodelling, phenotypic profile of stromal cells, cancer growth and therapeutic response. The stromal fibroblast populations of the TME include normal fibroblasts and melanoma-associated fibroblasts (MAFs) that are highly abundant and flexible cell types interacting with melanoma and stromal cells and differently influencing CM outcomes. The shift from the normal microenvironment to TME and from normal fibroblasts to MAFs deeply sustains CM growth. Hence, in this article we review the features of the normal microenvironment and TME and describe the phenotypic plasticity of normal dermal fibroblasts and MAFs, highlighting their roles in normal skin homeostasis and TME regulation. Moreover, we discuss the influence of MAFs and their secretory profiles on TME remodelling, melanoma progression, targeted therapy resistance and immunosurveillance, highlighting the cellular interactions, the signalling pathways and molecules involved in these processes.
Proteomic Profile of Saliva in Parkinson’s Disease Patients: A Proof of Concept Study

Parkinson’s disease (PD) is a progressive neurodegenerative disorder. It affects many organs. Lewy bodies—a histopathological “hallmark” of PD—are detected in about 75% of PD submandibular gland samples. We hypothesize that saliva can be a source of biomarkers of PD. The aim of the study was to evaluate and compare the salivary proteome of PD patients and healthy controls (HC). Salivary samples from 39 subjects (24 PD patients, mean age 61.6 ± 8.2; 15 HC, mean age 60.9 ± 6.7) were collected. Saliva was collected using RNA-Pro-Sal kits. Label-free LC-MS/MS mass spectrometry was performed to characterize the proteome of the saliva. IPA analysis of upstream inhibitors was performed. A total of 530 proteins and peptides were identified. We observed lower concentrations of S100-A16, ARP2/3, and VPS4B in PD group when compared to HC. We conclude that the salivary proteome composition of PD patients is different than that of healthy controls. We observed a lower concentration of proteins involved in inflammatory processes, exosome formation, and adipose tissue formation. The variability of expression of proteins between the two groups needs to be considered.
A Review of Vision-Based On-Board Obstacle Detection and Distance Estimation in Railways

This paper provides a review of the literature on vision-based on-board obstacle detection and distance estimation in railways. Environment perception is crucial for autonomous detection of obstacles in a vehicle’s surroundings. The use of on-board sensors for road vehicles for this purpose is well established, and advances in Artificial Intelligence and sensing technologies have motivated significant research and development in obstacle detection in the automotive field. However, research and development on obstacle detection in railways has been less extensive. To the best of our knowledge, this is the first comprehensive review of on-board obstacle detection methods for railway applications. This paper reviews currently used sensors, with particular focus on vision sensors due to their dominant use in the field. It then discusses and categorizes the methods based on vision sensors into methods based on traditional Computer Vision and methods based on Artificial Intelligence.
Threading the needle: How humans influence predator-prey spatiotemporal interactions in a multiple-predator system.

Perceived predation risk and the resulting antipredator behavior varies across space, time, and predator identity. Communities with multiple predators that interact and differ in their use of space, time of activity, and hunting mode create a complex landscape for prey to avoid predation. Anthropogenic presence and disturbance have the potential to shift interactions among predators and prey and the where and when encounters occur. We examined how white-tailed deer (Odocoileus virginianus) fawn spatiotemporal antipredator behavior differed along an anthropogenic disturbance gradient that had black bears (Ursus americanus), coyotes (Canis latrans), bobcats (Lynx rufus), and humans present. We quantified 1) spatial co-occurrence in species distributions, 2) temporal overlap across the diel cycle, and 3) spatiotemporal associations between humans, bears, coyotes, bobcats, adult male deer, and fawns. We also examined how deer vigilance behavior changed across the anthropogenic disturbance gradient and survey duration. Anthropogenic disturbance influenced spatiotemporal co-occurrence across multiple scales, often increasing spatiotemporal overlap among species. In general, species' spatial co-occurrence was neutral or positive in anthropogenically disturbed environments. Bears and fawns, coyotes and adult male deer, and bobcats and fawns all had higher temporal overlap in the agriculture-development matrix sites. In addition, factors that influenced deer vigilance (e.g., distance to forest edge and predator relative abundance) in the agriculture-development matrix sites did not in the forest matrix site. By taking into account the different antipredator behaviors that can be detected and the different scales these behaviors might occur, we were able to gain a more comprehensive picture of how humans reduce available niche space for wildlife, creating the neutral and positive spatiotemporal associations between species that studies have been seeing in more disturbed areas.
Venous malformation vessels are improperly specified and hyperproliferative

Venous malformations (VMs) are slow-flow malformations of the venous vasculature and are the most common type of vascular malformation with a prevalence of 1%. Germline and somatic mutations have been shown to contribute to VM pathogenesis, but how these mutations affect VM pathobiology is not well understood. The goal of this study was to characterize VM endothelial and mural cell expression by performing a comprehensive expression analysis of VM vasculature. VM specimens (n = 16) were stained for pan-endothelial, arterial, venous, and endothelial progenitor cell proteins; proliferation was assessed with KI67. Endothelial cells in the VM vessels were abnormally orientated and improperly specified, as seen by the misexpression of both arterial and endothelial cell progenitor proteins not observed in control vessels. Consistent with arterialization of the endothelial cells, VM vessels were often surrounded by multiple layers of disorganized mural cells. VM endothelium also had a significant increase in proliferative endothelial cells, which may contribute to the dilated channels seen in VMs. Together the expression analysis indicates that the VM endothelium is misspecified and hyperproliferative, suggesting that VMs are biologically active lesions, consistent with clinical observations of VM progression over time.
Be Mindful of Your Metaphors about Microbes

Metaphors are ubiquitous in science and have important implications for how we frame our research objectives as well as how we communicate to the public. This piece focuses on the power of metaphors to shape our attitude and actions toward antimicrobial-resistant bacteria. ABSTRACT Metaphors are ubiquitous in science and have important implications for how we frame our research objectives as well as how we communicate to the public. This piece focuses on the power of metaphors to shape our attitude and actions toward antimicrobial-resistant bacteria. It begins by emphasizing the pervasiveness of war metaphors to describe bacteria. Then it highlights that, with this type of framing, the solutions follow a similar suit. Ultimately, this metaphorical framing can imply dangerously incorrect solutions to the problem of antibiotic resistance. I propose that we need metaphors that represent the problem of antimicrobial resistance as an ecological and evolutionary issue rather than a single bacterial enemy. I end by offering a new metaphor that does not downplay the healthy fear we should have for antimicrobial-resistant bacteria but acknowledges that living things evolve and self-preserve. This piece is a call to action to use metaphors that express microbes’ exceptional resilience rather than our brute strength in combat against them.
Laterality 2020: Response to the article commentaries

ABSTRACT In our recent opinion paper “Laterality 2020: entering the next decade”, we highlighted trends that we thought are likely to shape laterality research in the 2020s. Our opinion paper inspired 11 commentaries by experts from several disciplines which discussed a wide range of topics complementing the 10 trends we identified in the opinion paper. In this reply, we summarize and discuss the 11 commentaries by clustering them into 3 different main topics. The topic that was covered by the largest number of commentaries was the role of comparative and evolutionary approaches in laterality research. Moreover, several comments focused on the ontogenesis of hemispheric asymmetries and the importance of reliability and validity in laterality research. Embracing the technical advances, research trends and controversies laid out in the commentaries will significantly improve our understanding of several of the core questions of laterality research.
Three-Month Outcomes of Myopic Astigmatism Correction With Small Incision Guided Human Cornea Treatment.

PURPOSE To evaluate refractive and visual outcomes of small incision guided human cornea treatment (SmartSight; SCHWIND eye-tech-solutions) in the treatment of myopic astigmatism with the use of a new femtosecond laser system. METHODS This retrospective, observational case series study included 104 eyes of 56 patients who underwent SmartSight to correct myopic astigmatism and completed the 3-month follow-up. Procedures were performed with a SCHWIND ATOS femtosecond laser. RESULTS Preoperatively, mean manifest spherical equivalent refraction was -6.12 ± 1.99 diopters (D) (range: -3.00 to -11.50 D) and astigmatism was 1.02 ± 0.56 D (range: 0.25 to 2.75 D). Three months postoperatively, it was +0.47 ± 0.32 D (range: -0.75 to +1.00 D) and 0.27± 0.22 D (range: 0.00 to 0.75 D), respectively (both P < .05). Spherical equivalent correction within ±0.50 D was achieved in 62 eyes (60%), and cylindrical correction in 90 eyes (87%). Preoperative corrected distance visual acuity (CDVA) was 20/20 or better in 94 eyes (90%) and postoperative uncorrected distance visual acuity was 20/20 or better in 96 eyes (92%). No eye lost two or more Snellen lines of CDVA. CONCLUSIONS Myopic astigmatism correction with Smart-Sight provided good results for efficacy, safety, predictability, and visual outcomes in the first 3 months of follow-up. [J Refract Surg. 2021;37(5):304-311. ].
Different glutamate sources and endogenous co‐agonists activate extrasynaptic NMDA receptors on amacrine cells of the rod pathway microcircuit

The NMDA receptors (NMDARs) expressed by AII and A17 amacrine cells, the two main inhibitory interneurons of the rod pathway microcircuit in the mammalian retina, are exclusively extrasynaptic, activated by ambient levels of glutamate, and molecularly distinct, with AII and A17 amacrines expressing GluN2B‐ and GluN2A‐containing receptors, respectively. This important sensory microcircuit thus provides a unique model to study the activation and function of extrasynaptic NMDARs. Here, we investigated the sources of glutamate and the endogenous co‐agonists (d‐serine or glycine) that activate these distinct populations of NMDARs. With acute slices from rat retina, we used whole‐cell voltage‐clamp recording and measurement of current noise to monitor levels of NMDAR activity. Pre‐incubation of retina with bafilomycin A1 (an inhibitor of neurotransmitter uptake into synaptic vesicles) abolished NMDAR‐mediated noise in AII, but not A17 amacrines, suggesting a vesicular source of glutamate activates AII NMDARs, whereas a non‐vesicular source activates A17 NMDARs. Pre‐incubation of retina with l‐methionine sulfoximine (an inhibitor of glutamine synthetase) also abolished NMDAR‐mediated noise in AII, but not A17 amacrines, suggesting a neuronal source of glutamate activates AII NMDARs, whereas a glial source activates A17 NMDARs. Enzymatic breakdown of d‐serine reduced NMDAR‐mediated noise in AII, but not A17 amacrines, suggesting d‐serine is the endogenous co‐agonist at AII, but not A17 NMDARs. Our results reveal unique characteristics of these two populations of extrasynaptic NMDARs. The differential and independent activation of these receptors is likely to provide specific contributions to the signal processing and plasticity of the cellular components of the rod pathway microcircuit.
Scavenging reactive oxygen species mimics the anoxic response in goldfish pyramidal neurons.

Goldfish are one of a few species able to avoid cellular damage during month-long periods in severely hypoxic environments. By suppressing action potentials in excitatory glutamatergic neurons, the goldfish brain decreases its overall energy expenditure. Co-incident with reductions in O2 availability is a natural decrease in cellular reactive oxygen species (ROS) generation, which has been proposed to function as part of a low oxygen signal transduction pathway. Therefore, using live-tissue fluorescence microscopy, we found that ROS production decreased by 10% with the onset of anoxia in goldfish telencephalic brain slices. Employing whole-cell patch-clamp recording, we found that like severe hypoxia the ROS scavengers N-acetyl cysteine (NAC) and MitoTEMPO, added during normoxic periods, depolarized membrane potential (severe hypoxia -73.6 to - 61.4 mV; NAC -76.6 to -66.2 mV; and MitoTEMPO -71.5 mV to -62.5 mV) and increased whole-cell conductance (severe hypoxia 5.7 to 8.0 nS; NAC 6 nS to 7.5 nS; and MitoTEMPO 6.0 nS to 7.6 nS). Also, in a subset of active pyramidal neurons these treatments reduced action potential firing frequency (severe hypoxia 0.18 Hz to 0.03 Hz; NAC 0.27 Hz to 0.06 Hz and MitoTEMPO 0.35 Hz to 0.08 Hz ). Neither severe hypoxia nor ROS scavenging impacted action potential threshold. The addition of exogenous hydrogen peroxide could reverse the effects of the antioxidants. Taken together, this supports a role for a reduction in [ROS] as a low oxygen signal in goldfish brain.
Presence of Listeria monocytogenes in Ready-to-Eat (RTE) meat products sold at retail stores in Costa Rica and analysis of contributing factors.

Listeria monocytogenes is a pathogenic bacterium associated with RTE meat products sold at the retail level. The objective of this research was to determine the prevalence of Listeria monocytogenes in RTE meat products sold at retail in Costa Rica and to study the factors associated with the levels of contamination; analyzed factors include hygienic practices within stores (cutting techniques, microbial contamination of products) and the behavior of the isolates (persistence against antimicrobials and transfer potential). A total of 190 samples of RTE meat products were collected and analyzed for the presence of coliforms and Listeria species. Isolates of L. monocytogenes were then evaluated in terms of resistance to disinfectants (quaternary ammonium compounds and chlorine) and their transfer potential from food contact surfaces (knife and cuttingboards). Overall Listeria spp. prevalence was 37,4% (71/190); L. innocua was present in 32,1% (61/190) of the products and L. monocytogenes was found in just 2,6% (5/190) of the samples. Most of the contaminated samples were cut with a knife at the moment of purchase (44,2%). When analyzing practices within the stores, it was observed that L. monocytogenes transfer from inoculated knife to "salchichón" was higher for samples cut right at the beginning of the experiment. Also, L. monocytogenes transfer from inoculated cuttingboards was independent of the number of slices but contamination from plastic was higher than wood. Regarding L. monocytogenes resistance to disinfectants, average reductions of 2,6 ± 1,1 log CFU/mL were detected after 6 minutes of exposure to 200 ppm of chlorine; however, chlorine resistance varied among the strains. Prevalence of L. monocytogenes in RTE meat products sold at retail could be associated with handling practices within the stores; further studies are necessary to estimate the impact of these practices on the overall risk for consumers.