Miracle-dz/UniFAR-FTUs huggingface dataset:
Rapidly progressive dementias and the treatment of human prion diseases

Importance of the field: Rapidly progressive dementia (RPD) has many possible etiologies and definitive treatment is reliant upon an accurate diagnosis from an appropriate diagnostic work-up. A large portion of the neurodegenerative causes of RPD are due to prion diseases (e.g., Creutzfeldt–Jakob disease). The study of prion diseases, for which there is no currently available treatment, has public health implications and is becoming increasingly more relevant to our understanding of other protein misfolding disorders including Alzheimer's disease, frontotemporal degeneration, and Parkinson's disease. Areas covered in this review: This article begins with an overview of the etiologies and diagnostic work-up of RPD followed by a detailed review of the literature concerning the treatment of human prion diseases (1971 to present). What the reader will gain: The reader will understand the differential diagnosis and work-up of RPD as it pertains to its treatment, as well as an in-depth understanding of treatments of human prion diseases. Take home message: An accurate diagnosis of the cause of RPD is of paramount importance when determining appropriate treatment. Most studies of the treatment for human prion diseases are case reports or case series, and results from only one randomized, placebo-controlled study have been reported in the literature (flupirtine). Studies have been hindered by disease heterogeneity and lack of standardized outcome measures. Although no effective prion disease treatment has been revealed through these studies, they provide important considerations for future studies.
Optimal Allocation of Variable Renewable Energy Considering Contributions to Security of Supply

Electricity markets are increasingly influenced by variable renewable energy such as wind and solar power, characterized by a pronounced weather-induced variability and imperfect predictability. As a result, the evaluation of the capacity value of variable renewable energy, i.e., its contribution to security of supply, gains importance. This paper develops a new methodology to endogenously determine the capacity value in large-scale investment and dispatch models for electricity markets. The framework allows balancing effects to be accounted for that arise due to the spatial distribution of generation capacities and interconnectors. The practical applicability of the methodology is shown with an application for wind power in Europe. We find that wind power can substantially contribute to security of supply in a decarbonized European electricity system in 2050, with regional capacity values ranging from 1-40%. Analyses that do not account for the temporal and spatial heterogeneity of the contribution of wind power to security of supply therefore lead to inefficient levels of dispatchable back-up capacity. Applying a wind power capacity value of 5% results in an overestimation of firm capacity requirements in Europe by 66 GW in 2050. This translates to additional firm capacity provision costs of 3.8 bn EUR per year in 2050, which represents an increase of 7%.
A retinoic acid response element is part of a pleiotropic domain in the phosphoenolpyruvate carboxykinase gene.

Several hormones, including insulin, glucagon, and glucocorticoids, regulate the expression of the rate-limiting gluconeogenic enzyme, phosphoenolpyruvate carboxykinase [GTP: oxaloacetate carboxy-lyase (transphosphorylating); EC 4.1. 1.32; PEPCK] in liver. In this report we demonstrate that retinoic acid (RA) also regulates PEPCK expression by inducing a 3-fold increase in the rate of transcription of the PEPCK gene. A RA response element located between -468 and -431 in the PEPCK promoter mediates a 7-fold increase in expression of a chimeric construct containing the basal PEPCK promoter ligated to the chloramphenicol acetyltransferase reporter gene. This element confers RA responsiveness through the heterologous thymidine kinase promoter and functions relatively independent of position and orientation. An 18-base-pair core sequence (-451 to -434) (i) mediates an effect of RA on PEPCK gene expression and contains motifs found in two other RA response elements; (ii) corresponds to AF1, an accessory factor element that is an integral component of the complex glucocorticoid response unit in the PEPCK gene promoter; (iii) is in a region involved in the developmental expression of the PEPCK gene; and (iv) shows homology to elements involved in the tissue-specific regulation of genes, including the hepatic apolipoprotein genes and the alpha 1-antitrypsin gene.
Calcified carotid atherosclerotic plaque is associated less with ischemic symptoms than is noncalcified plaque on MDCT.

OBJECTIVE Risk assessment based on plaque vulnerability would be valuable in the management of asymptomatic carotid stenosis. The purpose of this study was to compare plaque morphology in symptomatic and asymptomatic patients with significant extracranial carotid artery stenosis using MDCT angiography. MATERIALS AND METHODS We identified 31 patients with greater than 60% carotid artery stenosis on MDCT angiography using the criteria of the North American Symptomatic Carotid Endarterectomy Trial Collaborators. We analyzed plaque density by blinded review in Hounsfield units in the atherosclerotic plaques of 15 symptomatic and 21 asymptomatic stenotic vessels for classification as soft, intermediate, or calcified. Data were analyzed using multiple logistic regression. RESULTS Even with age, traditional cardiovascular risk factors, and treatment taken into account, we found that calcified plaques were 21 times less likely to be symptomatic than noncalcified plaques (95% confidence interval for odds ratio, 0.003, 0.749; p = 0.030). No significant predictive value was found between soft (p = 0.23) or intermediate (p = 0.18) plaque morphology for the occurrence of symptoms. CONCLUSION MDCT angiography may help risk-stratify patients with asymptomatic carotid artery stenosis. Extracranial carotid artery calcified plaques causing stenosis are significantly less likely to be symptomatic and thus may be more stable than noncalcified plaques. This finding may have implications for the interpretation of calcification of atherosclerotic plaque in other vascular beds.
FASS is a Better Predictor of Poor Outcome in Lower Limb Blast Injury Than AIS: Implications for Blast Research

Objectives Due to the absence of clinical blast data, automotive injury data using the abbreviated injury score (AIS) has been extrapolated to define current North Atlantic Treaty Organisation (NATO) injury thresholds for anti-vehicle mine tests. We hypothesized that AIS, being a marker of fatality rather than disability, would be a worse predictor of poor clinical outcome compared with the lower limb–specific foot and ankle severity score (FASS). Methods Using a prospectively collected trauma database, we identified UK Service Personnel sustaining lower leg injuries from under-vehicle explosions from January 2006 to December 2008. A full review of all medical documentation was performed to determine patient demographics and the severity of lower leg injury, as assessed by AIS and FASS. Clinical endpoints were defined as (1) need for amputation or (2) poor clinical outcome (defined as amputation or ongoing clinical problems). Statistical models were developed to explore the relationship between the scoring systems and clinical endpoints. Results Sixty-three UK casualties (89 limbs) were identified with a lower limb injury after under-vehicle explosion. The mean age of the casualty was 26.0 years. At 33.6 months follow-up, 29.1% (26 of 89) required an amputation and 74.6% (67 of 89) having a poor clinical outcome. Only 9 (14%) casualties were deemed medically fit to return to full military duty. Receiver operating characteristic analysis revealed that both AIS = 2 and FASS = 4 could predict the risk of amputation, with FASS = 4 demonstrating greater specificity (43% vs. 20%) and greater positive predictive value (72% vs. 34%). In predicting poor clinical outcome, FASS was significantly superior to AIS. Probit analysis revealed that a relationship could not be developed between AIS and the probability of a poor clinical outcome. Conclusions Our study clearly demonstrates that AIS is not a predictor of long-term clinical outcome and that FASS would be a better quantitative measure of lower limb injury severity.
High CJD infectivity remains after prion protein is destroyed

The hypothesis that host prion protein (PrP) converts into an infectious prion form rests on the observation that infectivity progressively decreases in direct proportion to the decrease of PrP with proteinase K (PK) treatment. PrP that resists limited PK digestion (PrP‐res, PrPsc) has been assumed to be the infectious form, with speculative types of misfolding encoding the many unique transmissible spongiform encephalopathy (TSE) agent strains. Recently, a PK sensitive form of PrP has been proposed as the prion. Thus we re‐evaluated total PrP (sensitive and resistant) and used a cell‐based assay for titration of infectious particles. A keratinase (NAP) known to effectively digest PrP was compared to PK. Total PrP in FU‐CJD infected brain was reduced to ≤0.3% in a 2 h PK digest, yet there was no reduction in titer. Remaining non‐PrP proteins were easily visualized with colloidal gold in this highly infectious homogenate. In contrast to PK, NAP digestion left 0.8% residual PrP after 2 h, yet decreased titer by >2.5 log; few residual protein bands remained. FU‐CJD infected cells with 10× the infectivity of brain by both animal and cell culture assays were also evaluated. NAP again significantly reduced cell infectivity (>3.5 log). Extreme PK digestions were needed to reduce cell PrP to <0.2%, yet a very high titer of 8 logs survived. Our FU‐CJD brain results are in good accord with the only other report on maximal PrP destruction and titer. It is likely that one or more residual non‐PrP proteins may protect agent nucleic acids in infectious particles. J. Cell. Biochem. 112: 3630–3637, 2011. © 2011 Wiley Periodicals, Inc.
Neuropsychiatric Complications after Cardiopulmonary Bypass: Cerebral Protection by a Barbiturate

The authors prospectively investigated the ability of thiopental to decrease neuropsychiatric complications as a consequence of openventricle operations requiring cardiopulmonary bypass. Eighty-nine randomly assigned patients received sufficient thiopental to maintain electroencephalographic silence throughout the period from before atrial cannulation to termination of bypass. These patients received an average of 39.5 mg/kg of thiopental, while 93 control patients received only fentanyl. On the first postoperative day, five thiopental (5.6%) and eight control (8.6%) patients exhibited clinical neuro-psychiatric abnormalities. By the tenth postoperative day, all neuropsychiatric dysfunction had resolved in the thiopental group but persisted in seven (7.5%) control patients (P < 0.025). The incidence of complications was significantly related to calcification of replaced valves, aortic valve replacement, advanced age, and prolonged bypass, but not to low blood pressure during perfusion. The authors believe their data are consistent with embolism as the most important cause of sensory-motor neurologic dysfunction following cardiopulmonary bypass. The data also provide evidence that thiopental in sufficient dosage can reduce the clinical consequences of these events. This is the first demonstration of cerebral protection by a barbiturate in humans.
Circuit models explaining bandwidth behavior of small circular cavity backed patch antennas

The objective of this work is to determine the orders of magnitude of the bandwidth available from a patch antenna enclosed in a small finite circular cavity. Numerical investigations were performed using commercial software where the antenna was optimized to resonate at 2.3GHz. We considered cavities with aperture diameters of 32mm (0.25 λ0), 40mm (0.3 λ0) and 50mm (0.38λ0) and substrate permittivity of 3.66, 6.15 and 10.2 inside the cavity volume. Results for bandwidth behavior are shown in dependence on cavity height up to the maximum height where matching the patch antenna with a probe feed was still possible. Bandwidth performance is also analyzed analytically using simple transmission line models that can describe the antenna behavior in a qualitative manner and explain several unexpected results shown in the paper. Numerical results for patch antennas are also compared to analytical predictions for a simple cavity antenna of the same size without a patch, assumed to be excited only by the fundamental waveguide mode.
SAR image change detection based on nonstationary analysis and conditional random fields

In this paper, we propose a synthetic aperture radar (SAR) image change detection algorithm based on SAR image nonstationary analysis and conditional random field (CRF) model. This algorithm segments the SAR images into two nonstationary regions with the Triplet Markov Field (TMF) model which incorporates a U field to describe the images' nonstationary nature. The combined posterior probability of the CRF model consists of two parts: the unary potential and the pairwise potential. The unary potential is modeled by support vector machine (SVM) using the texture features extracted from the two-temporal SAR images, and the pairwise potential is constructed by the multilevel logistical model to capture the spatial interactions of the log-ratio image. Both the unary and pairwise potentials are improved with the introduction of the U field. The parameters of the pairwise potential are estimated by the iterative conditional estimation (ICE) algorithm. The iterative conditional model (ICM) algorithm is utilized for updating the combined posterior probability, and the final change detection results are obtained when it converges. Finally, experimental results on four two-temporal SAR images validate the effectiveness of the proposed image change detection algorithm.
Human papillomavirus infection: biology, epidemiology, and prevention

Over the past several decades, knowledge of the biology and epidemiology of human papillomavirus (HPV) infection has increased tremendously. However, there are still many unanswered questions concerning the interaction of the virus with its host. The virus has been identified as a necessary causal agent for cervical squamous neoplasia and has been linked to the development of neoplasia in several other mucosal sites. The viral oncogenes E6 and E7 are the major players in the virus' scheme to evade the immune system and use the host cell replication machinery to survive. Many risk factors for infection with HPV have been identified; however, the focus now centers on identifying risk factors for persistence of the infection as it is likely that transient infections play a very small role in the overall development of clinical disease. Prevention measures to date have centered around screening programs, mostly for cervical cancer, including the perfection of screening techniques and inclusion of molecular testing for HPV into screening regimens. The development of prophylactic and therapeutic vaccines has also increased as primary prevention measures appear to have the best hope for long-term effects on cancer incidence.
Chondroitin sulfate proteoglycan surrounds a subset of human and rat CNS neurons

Chondroitin sulfate proteoglycan (CS‐PG) bearing glycosaminoglycan (GAG) chains containing unsulfate (COS) and 6‐sulfate (C6S) disaccharides was immunolocalized in rat and human CNS by using monoclonal antibodies (MAb) specific for the two disaccharides. The immunostaining with both MAb was restricted to the periphery of a neuronal subset in rat and human CNS. Double immunofluorescence showed codistribution of the antigens around the same neuronal population. The staining with anti‐COS MAb was stronger than with anti‐C6S MAb, suggesting that the proteoglycan (PG) contains mainly COS disaccharjdes. In different rat cortical areas, 40–;60/mm2 positive interneurons were found, the visual cortex showing the highest value. In human cortex, positivity was also observed around the soma of some pyramidal cells. In the rat, positive neurons were also localized in deep cerebellar nuclei, reticular nucleus of the thalamus, and other structures of the midbrain and hindbrain. CA3 region of hippocampus and the external layer of pyriform cortex were characterized by positivity of the neuropil.
Cystic Echinococcosis

ABSTRACT Echinococcosis is one of the 17 neglected tropical diseases (NTDs) recognized by the World Health Organization. The two major species of medical importance are Echinococcus granulosus and Echinococcus multilocularis. E. granulosus affects over 1 million people and is responsible for over $3 billion in expenses every year. In this minireview, we discuss aspects of the epidemiology, clinical manifestations, and diagnosis of cystic echinococcosis or cystic hydatid disease caused by E. granulosus.
Hsp72 is an early and sensitive biomarker to detect acute kidney injury

This study was designed to assess whether heat shock protein Hsp72 is an early and sensitive biomarker of acute kidney injury (AKI) as well as to monitor a renoprotective strategy. Seventy‐two Wistar rats were divided into six groups: sham‐operated and rats subjected to 10, 20, 30, 45 and 60 min of bilateral ischemia (I) and 24 h of reperfusion (R). Different times of reperfusion (3, 6, 9, 12, 18, 24, 48, 72, 96 and 120 h) were also evaluated in 30 other rats subjected to 30 min of ischemia. Hsp72 messenger RNA (mRNA) and protein levels were determined in both kidney and urine. Hsp72‐specificity as a biomarker to assess the success of a renoprotective intervention was evaluated in rats treated with different doses of spironolactone before I/R. Renal Hsp72 mRNA and protein, as well as urinary Hsp72 levels, gradually increased relative to the extent of renal injury induced by different periods of ischemia quantified by histomorphometry as a benchmark of kidney damage. Urinary Hsp72 increased significantly after 3 h and continued rising until 18 h, followed by restoration after 120 h of reperfusion in accord with histopathological findings. Spironolactone renoprotection was associated with normalization of urinary Hsp72 levels. Accordingly, urinary Hsp72 was significantly increased in patients with clinical AKI before serum creatinine elevation. Our results show that urinary Hsp72 is a useful biomarker for early detection and stratification of AKI. In addition, urinary Hsp72 levels are sensitive enough to monitor therapeutic interventions and the degree of tubular recovery following an I/R insult.
Catheter Ablation of Recurrent Scar‐Related Ventricular Tachycardia Using Electroanatomical Mapping and Irrigated Ablation Technology: Results of the Prospective Multicenter Euro‐VT‐Study

Catheter Ablation of Ventricular Tachycardia. Introduction: Ventricular tachycardia (VT) late after myocardial infarction is an important contributor to morbidity and mortality. This prospective multicenter study assessed the efficacy and safety of electroanatomical mapping in combination with open‐saline irrigated ablation technology for ablation of chronic recurrent mappable and unmappable VT in remote myocardial infarction.
Physical Education - Based Teaching Personal Social Responsibility

The general purpose of this study is to examine the effect of Physical Education learning based on Teaching personal Social Responsibility in increasing student's responsibility level. This study uses experimental Quasi with pre-test post-test control group design. The subjects of the study were male and female high school students of grade 11 who were between 15-17 years old. The instrument used is Tools for Assessing Responsibility Based Physical education (TARE). Quantitative analysis of Paired Sample t-test and independent sample t-test will be conducted to explain validation testing of this research. The results showed that physical education learning based on TPSR had a significant effect on the improvement of student responsibility level. The results of this study conclude that Teaching Personal Social responsibility model is suitable to be implemented in physical education in high school and give significant benefit in increasing student responsibility level.
Pancreatic duct: morphologic and functional evaluation with dynamic MR pancreatography after secretin stimulation.

PURPOSE To assess the utility of dynamic MR pancreatography in the evaluation of the behavior of the pancreatic duct after secretin stimulation and to estimate pancreatic exocrine reserve in patients suspected of having acute recurrent or chronic pancreatitis. MATERIALS AND METHODS Ten healthy volunteers and 13 patients suspected of having pancreatic disease and no obvious markers of chronic pancreatitis were studied. Single-shot turbo spin-echo T2-weighted dynamic MR pancreatograms were obtained before and every 30 seconds during the 10 minutes after secretin administration. Morphologic features and diameter of the pancreatic duct were monitored before and during secretin stimulation. Duodenal filling volume was graded. Results were compared with those of endoscopic retrograde cholangiopancreatography and secretin stimulation testing with collection of pancreatic fluid. RESULTS Secretin improved the delineation of ductal morphologic features in both groups. Persistent dilatation of the pancreatic duct after stimulation (five patients) was a significant finding (P = .0015) in the diagnosis of papillary stenosis. The mean duodenal filling score was significantly lower in patients with reduced exocrine function (six patients) than that in the volunteers (P < .0001). CONCLUSION The use of dynamic MR pancreatography with secretin stimulation may be useful for diagnosing pancreatic papillary stenosis or dysfunction and for detecting reduced pancreatic exocrine reserve.
Rethinking Bullying Interventions for High School Students: A Qualitative Study

The purpose of this study was to obtain feedback to inform a modification of a brief, school-based bullying intervention originally developed for elementary and middle school students to be age appropriate for the high school level. We investigated the in-depth, inner experiences of high school students randomly selected to participate in a brief, bystander bullying intervention program. Thorough qualitative analysis, we found students spoke about (a) the complexity of bullying in high school, (b) cyberbullying and increased potential consequences related to social media, (c) the normalization and minimization of bullying, (d) lack of adult support, (e) an appreciation for the program’s strategies for students to take action, and (f) the importance of training activities to help build rapport and increase awareness. We discuss implications for counselors and counselor educators based on our findings.