Miracle-dz/UniFAR-FTUs huggingface dataset:
Hydrogen Peroxide and Lipopolysaccharide Differentially Affect the Expression of MicroRNAs 10a, 33a, 21, 221 in Endothelial Cells Before and After Coculture With Monocytes

Inflammation and oxidative stress are important risk factors affecting various cells in the formation of atherosclerosis. MicroRNAs (miRs) are regulators of inflammation and atherogenesis. The expressions of endothelial cell (EC)-specific miR-10a and miR-21 and monocyte-specific miR-33a and miR-221 were investigated using coculture of the ECs and monocytes upon exposure to H2O2 as an oxidative stressor, and endotoxin/lipopolysaccharide (LPS) as a microbial stressor. Human umbilical endothelial cells (HUVECs) and peripheral blood mononuclear cells (or monocytes) were cocultured in M199 complete medium and were incubated with LPS (20 ng/mL) or H2O2 (1%) for 8 hours at 37°C. The HUVECs and monocytes were then separated from the cellular mix using a magnetic bead negative selection technique. The relative expression of miRs was determined by real-time polymerase chain reaction. In both cell types, H2O2 induced miR10a (P = 0.05) and LPS induced miR21 (P = 0.0003) compared to the untreated controls. Coculture increased miR-10a and miR-21 expression in monocytes (P = 0.0008 and <0.0001); however when cultured alone, HUVECs expressed higher levels of miR-10a and miR-21 (P < 0.0001 and <0.0001). Coculture decreased the expression of miR-33a in monocytes (P < 0.0001) while increasing miR221 in HUVECs and monocytes (P < 0.0001 and <0.0001). The expression pattern of miRs in HUVECs and monocytes changes in the coculture compared to culturing alone in response to oxidative and microbial toxic compounds. Moreover, different cellular stressors induce different athero-miRs, which may affect the course of inflammation.
TACI attenuates antibody production costimulated by BAFF‐R and CD40

B cell activating factor of the TNF family (BAFF), plays critical roles in B cell survival, activation, differentiation, and antibody (Ab) production. BAFF binds to three receptors: BAFF‐R, transmembrane activator and calcium‐modulator and cyclophilin ligand interactor (TACI) and B cell maturation antigen. While BAFF‐R is the primary receptor for B cell costimulation by BAFF, TACI is reported to serve as a positive or negative regulator for B cell responses depending on conditions. To determine the real role of TACI in B cell responses, we examined the functional relationship between TACI and BAFF‐R in Ab production from human peripheral blood B cells using agonistic mAb. BAFF‐R and CD40 enhanced IgG secretion and B cell proliferation, which were inhibited by TACI. Although TACI induced mild B cell apoptosis, its extent did not correlate with that of TACI‐mediated inhibition of IgG secretion. In addition, TACI inhibited B‐lymphocyte‐induced maturation protein‐1 expression, IgG secretion from previously IgG‐negative selected B cells, and activation‐induced cytidine deaminase expression enhanced by BAFF‐R and CD40. Importantly, BAFF‐R and CD40 enhanced B cell responsiveness to TACI‐mediated suppression. Thus, BAFF may attenuate T cell‐independent and ‐dependent B cell responses by TACI.
Perceived necessity explains the dissociation between logic and meaning: the case of "only if".

The purpose of this article was to investigate why sentences of the form "p only if q" often appear to have a different meaning than sentences of the form "if p, then q," despite their logical equivalence. The results of 3 experiments indicate that when "if . then" statements were equated with respect to necessity and temporal relations, different pragmatic relations (such as permission, causation, etc.) elicited similar "only if" judgments. However, different necessity relations elicited different "only if" judgments, regardless of the type of pragmatic relation expressed in the "if then" statement. These data suggest that "only if" judgements are primarily mediated by necessity and temporal relations and that pragmatic contexts may play a more indirect role, such as in the interpretation of necessity and temporal relations. Suggestions for how these findings might be incorporated into pragmatic schema theory (P. W. Cheng & K. J. Holyoak, 1985; P. W. Cheng, K. J. Holyoak, R. E. Nisbett, & L. M. Oliver, 1986) and mental models theory (P. N. Johnson-Laird & R. M. J. Byrne, 1991) are discussed.
Vitamin D Insufficiency and Deficiency among HIV-1-Infected Patients in a Tropical Setting

Vitamin D plays role in bone health and the regulation of the immune system. A cross-sectional study of serum 25-hydroxyvitamin D (25[OH]D) levels was conducted among HIV-1-infected Thai patients to determine the prevalence and associated factors of low vitamin D levels (25[OH]D <30 ng/mL) in tropical setting. 25-Hydroxyvitamin D was measured by liquid chromatography/tandem mass spectrometry. Of 178 patients, 58% received antiretroviral therapy at median (interquartile range [IQR]) duration of 7.4 (5.9-8.5) years. The prevalence of 25(OH)D deficiency (<20 ng/mL) and insufficiency (20-29.9 ng/mL) was 26.8% and 44.9%, respectively. Multivariate analysis showed that receiving efavirenz (EFV) was significantly associated with low vitamin D status (odds ratio = 3.60; 95% confidence interval, 1.06-12.15, P <.05). The mean (±standard deviation) level of 25(OH)D in patients receiving and not receiving EFV was 22.9 (6.6) and 28.6 (10.7) ng/mL, respectively, (P <.05). Low vitamin D status is common and needs to be assessed among HIV-infected patients including tropical residents especially when EFV is used.
Brand Analysis in Social Network Services: Results from Content Analysis in Twitter Regarding the US Smartphone Market

Marketing is the front-end strategy of firms to communicate the value of their products or services to customers; therefore, innovations in marketing have tremendous value in comparison to the whole innovation strategy of firms. The emergence of social network services (SNSs) as a dominant communication platform among firms and users provides an opportunity to evaluate the innovativeness of a firm’s marketing strategy. With an analysis of Twitter data, the study indicates how users react to content from different profile types. This result could inspire firms and the social media strategists of companies to diversify their content over multiple user profiles.
Building the education revolution (BER) program: governing the implementation of project policies

The project world is plagued with the interest and influence of diverse stakeholders shaping major project policy strategies. From deception, strategic manipulation to illusions of control, stakeholders using power and any other means to influence project strategies to achieve their desired ends: the tribalism and Machiavellianism of the project world. Governing tribalism or Machiavellianism especially at the front-end of projects is absolutely essential for project policy success. But how does a project manager shape a major project policy into success? The purpose of this research is to investigate tribalism and Machiavellianism and implement the necessary governance mechanisms to shape the implementation of project policies to optimize its success. This requires a deep understanding of human relational behavior. A case study approach was adopted that investigated Australia's largest infrastructure program in history, the AUD $16.2 billion Building the Education Revolution (BER) program, which was plagued with power and politics and Machiavellianism of the project world. Factors such as collective institutional leadership with formal and informal governance mechanisms in rationalizing decisions were identified as essential aspects from the case study. It was also revealed that if the factors are implemented at the front-end of project policies and managed appropriately it could significantly limit the potentness of the tribal or Machiavelli plague and optimize the chances of project policy success.
Minimization of Torque Ripple and Copper Losses in Switched Reluctance Drive

This paper presents a control technique for torque-ripple minimization in the switched reluctance motor (SRM) drive, based on a torque-sharing function (TSF) concept. In the proposed method, the reference torque is directly translated into the reference current waveform using the analytical expression. Optimization criteria of a TSF that are concerned with secondary objectives, such as minimization of copper losses or maximization of drive performance, are described. In addition, a novel family of TSFs is introduced. An optimal TSF can be easily extracted from the proposed family to satisfy one of the secondary objectives or to create balance between more of them. Control performances of the two extracted TSFs and the two optimized conventional (linear and sinusoidal) TSFs are compared. These four TSFs keep the copper losses to nearly the theoretical minimum. Each of them provides approximately the same operation efficiency of the considered three-phase 6/4 SRM drive. However, due to extension of the commutation angle between adjacent phases, TSFs from the proposed family provide better torque-speed characteristics. Moreover, one of them expands the possible speed range of torque-ripple-free drive operation, and another one, which provides the best torque-speed characteristics, reduces the peak phase current.
Targeting of protein translation as a new treatment paradigm for prostate cancer

Purpose of review The current overview will summarize some of the developments in the area of protein translation, including their relation to the therapeutic targeting of prostate cancer. Recent findings Translational control, mediated by the rate-limiting eukaryotic translation initiation factor 4E (eIF4E), drives selective translation of several oncogenic proteins, thereby contributing to tumor growth, metastasis, and treatment resistance in various cancers, including prostate cancer. As an essential regulatory hub, several oncogenic hyperactive signaling pathways appear to converge on eIF4E to promote tumorigenesis. Several approaches that target the eIF4E-dependent protein translation network are being actively studied, and it is likely that some may ultimately emerge as promising anticancer therapeutics. Summary An array of inhibitors has shown promise in targeting specific components of the translational machinery in several preclinical models of prostate cancer. It is hoped that some of these approaches may ultimately have relevance in improving the clinical outcomes of patients with advanced prostate cancer.
Stratified Random Forest for Genome-wide Association Study

For high dimensional genome-wide association (GWA) case-control data of complex disease, there are usually a large portion of single-nucleotide polymorphisms (SNPs) that are irrelevant with the disease. A simple random sampling method in random forest using default mtry parameter to choose feature subspace, will select too many subspaces without informative SNPs. Exhaustive searching an optimal mtry is often required in order to include useful and relevant SNPs and get rid of vast of non-informative SNPs. However, it is very time-consuming and not favorable in GWA study for high dimensional data. This paper proposes a stratified sampling method for feature subspace selection to generate decision trees in a random forest for GWA high-dimensional data. We employ two genome-wide SNP data sets (Parkinson case control data comprised of 408,803 SNPs and Alzheimer case control data comprised of 380,157 SNPs) to demonstrate that the proposed stratified sampling method is effective, and it can generate better random forest with higher accuracy and lower error bound than those by Breiman's random forest generation method.
Reconstitution of Protective Immune Responses against Cytomegalovirus and Varicella Zoster Virus Does Not Require Disease Development in Pediatric Recipients of Umbilical Cord Blood Transplantation

CMV and varicella zoster virus (VZV) are significant causes of morbidity and mortality following umbilical cord blood transplantation (UCBT). However, the kinetics of reconstitution and protective potential of antiviral cell-mediated immune responses following UCBT remain poorly characterized. In this study, the reconstitution of CMV- and VZV-specific T cell responses was assessed using IFN-γ ELISPOT in 28 children who underwent UCBT to treat hematological or inherited disorders. Barely detectable in the first 3 mo posttransplantation, CMV- and VZV-specific T cell responses were observed in 30.4% and 40.3% of study subjects after 36 mo of follow-up. Four of five CMV-seropositive subjects developed detectable levels of circulating CMV DNA (DNAemia), and 5 of 17 VZV-seropositive patients experienced herpes zoster during the posttransplant period. Four CMV-seronegative subjects developed IFN-γ responses against CMV, and four subjects developed a VZV-specific IFN-γ response without clinical signs of infection. No CMV- or VZV-related events were observed in study subjects following the development of CMV- or VZV-specific responses > 150 spot-forming units/106 PBMCs, consistent with T cell-mediated protection. Finally, famciclovir prophylaxis did not strictly prevent the reconstitution of the VZV-specific T cell repertoire, because the frequency of T cells producing IFN-γ in response to VZV Ags reached levels consistent with protection in two nonzoster subjects. Monitoring of CMV- and VZV-specific cell-mediated immunity could inform immunocompetence and guide the initiation and cessation of antiherpetic prophylaxis in UCBT recipients.
28 Friendship, housing environment and economic resources: what influences social network size after age 50?

“No man is an island” wrote the poet John Donne. Even economists, the champions of atomistic agents maximising their utility, have developed formal models of altruism, household bargaining, reciprocity, or peer effects. More simply, social ties have been shown to be important for a variety of economic and non-economic outcomes and they are likely to be particularly relevant in old age when help from others becomes a necessity. Bonsang and van Soest (2012) motivate their study of the satisfaction with social contacts of older Europeans by referring to various studies on the strong link between satisfaction with social life and subjective well-being (Van Praag & Ferrer-i-Carbonell 2008). In this chapter we take a different perspective. Rather than assessing the importance of social networks, we focus on the determinants of their size. Are social networks a purely personal affair, or are they influenced by factors such as the housing environment where a person lives? From the novels of Dickens and the reports of Villermé in the 19th century to the fundraising of Abbé Pierre in post WWII France, many have stressed the morbid influence of slums, or of lack of sun and space. More recently the “Moving to Opportunity for Fair Housing” randomised experiment in the U.S.A. demonstrated the programme’s greater long term beneficial impact on health and subjective well-being rather than on economic outcomes (Ludwig et al. 2008). Studies on the influence of the environment on older individuals’ well-being are less common (Cagney et al. 2009, Everson-Rose et al. 2011). If well-being depends on having many social ties and if the environment influences the number of social ties, an issue for economic policy, especially in a time of economic crisis,
Cumulative Incidence of False-Positive Results in Repeated, Multimodal Cancer Screening

PURPOSE Multiple cancer screening tests have been advocated for the general population; however, clinicians and patients are not always well-informed of screening burdens. We sought to determine the cumulative risk of a false-positive screening result and the resulting risk of a diagnostic procedure for an individual participating in a multimodal cancer screening program. METHODS Data were analyzed from the intervention arm of the ongoing Prostate, Lung, Colorectal, and Ovarian (PLCO) Cancer Screening Trial, a randomized controlled trial to determine the effects of prostate, lung, colorectal, and ovarian cancer screening on disease-specific mortality. The 68,436 participants, aged 55 to 74 years, were randomized to screening or usual care. Women received serial serum tests to detect cancer antigen 125 (CA-125), transvaginal sonograms, posteroanterior-view chest radiographs, and flexible sigmoidoscopies. Men received serial chest radiographs, flexible sigmoidoscopies, digital rectal examinations, and serum prostate-specific antigen tests. Fourteen screening examinations for each sex were possible during the 3-year screening period. RESULTS After 14 tests, the cumulative risk of having at least 1 false-positive screening test is 60.4% (95% CI, 59.8%–61.0%) for men, and 48.8% (95% CI, 48.1%–49.4%) for women. The cumulative risk after 14 tests of undergoing an invasive diagnostic procedure prompted by a false-positive test is 28.5% (CI, 27.8%–29.3%) for men and 22.1% (95% CI, 21.4%–22.7%) for women. CONCLUSIONS For an individual in a multimodal cancer screening trial, the risk of a false-positive finding is about 50% or greater by the 14th test. Physicians should educate patients about the likelihood of false positives and resulting diagnostic interventions when counseling about cancer screening.
Increased blood–brain barrier permeability in type II diabetes demonstrated by gadolinium magnetic resonance imaging

Objectives: Patients with type II diabetes are at increased risk of cognitive impairment. The retinal and renal complications of diabetes follow microvascular damage permitting small arterioles to leak, hence the cerebral damage might also follow loss of blood–brain barrier (BBB) integrity. Magnetic resonance (MR) brain imaging with intravenous gadolinium (Gd) diethylenetriamine pentaacetic acid (Gd-DTPA) was used to identify increased BBB permeability. Methods: Ten well controlled type II diabetic patients aged 65–70 years and 10 controls underwent MR brain imaging with fluid attenuated inversion recovery (FLAIR); T1 weighted (T1W) volumetric imaging before; and T1W volumetric imaging at 5, 15, 30, 45, 60, and 90 minutes after intravenous Gd-DTPA. The T1W image before Gd-DTPA was subtracted from the images at each time point after Gd-DTPA. Net signal intensity was plotted against time for different brain regions. White matter hyperintensities were scored from the FLAIR image. Results: The signal intensity/time curves showed that brain signal intensity increased more in the diabetic group than controls during the first 15 minutes after Gd-DTPA, particularly in the basal ganglia (p=0.018). Signal intensity in controls peaked at five minutes and diabetics at 15 minutes. Subjects with more white matter hyperintensities had greater signal increase after Gd-DTPA, whether diabetic or not (p=0.001). Conclusions: Increased BBB permeability with MR imaging was detected in patients with type II diabetes or white matter hyperintensities. Increased permeability of the BBB might account for some of the cerebral effects of type II diabetes, and so possibly also for the effect of other conditions that affect the microvasculature (like hypertension), on the brain.
APOBEC3 proteins and genomic stability

The human APOBEC3 family of cytidine deaminases constitutes a cellular intrinsic defense mechanism that is effective against a range of viruses and retro-elements. While it is well established that these enzymes are powerful mutators of viral DNA, the possibility that their activity could threaten the integrity of the host genome has only recently begun to be investigated. Here, we discuss the implications of new evidence suggesting that APOBEC3 proteins can mediate the deamination of cellular DNA. The maintenance of genomic integrity in the face of this potential off-target activity must require high fidelity DNA repair and strict regulation of APOBEC3 gene expression and enzyme activity. Conversely, the ability of specific members of the APOBEC3 family to activate DNA damage signaling pathways might also reflect another way that these proteins contribute to the host immune response.
Wnt/β-Catenin/Tcf Signaling Induces the Transcription of Axin2, a Negative Regulator of the Signaling Pathway

ABSTRACT Axin2/Conductin/Axil and its ortholog Axin are negative regulators of the Wnt signaling pathway, which promote the phosphorylation and degradation of β-catenin. While Axin is expressed ubiquitously, Axin2 mRNA was seen in a restricted pattern during mouse embryogenesis and organogenesis. Because many sites of Axin2 expression overlapped with those of several Wnt genes, we tested whether Axin2 was induced by Wnt signaling. Endogenous Axin2 mRNA and protein expression could be rapidly induced by activation of the Wnt pathway, and Axin2 reporter constructs, containing a 5.6-kb DNA fragment including the promoter and first intron, were also induced. This genomic region contains eight Tcf/LEF consensus binding sites, five of which are located within longer, highly conserved noncoding sequences. The mutation or deletion of these Tcf/LEF sites greatly diminished induction by β-catenin, and mutation of the Tcf/LEF site T2 abolished protein binding in an electrophoretic mobility shift assay. These results strongly suggest that Axin2 is a direct target of the Wnt pathway, mediated through Tcf/LEF factors. The 5.6-kb genomic sequence was sufficient to direct the tissue-specific expression of d2EGFP in transgenic embryos, consistent with a role for the Tcf/LEF sites and surrounding conserved sequences in the in vivo expression pattern of Axin2. Our results suggest that Axin2 participates in a negative feedback loop, which could serve to limit the duration or intensity of a Wnt-initiated signal.
Guided self-help for bulimia nervosa in primary care: a randomized controlled trial

Background. To increase access to cognitive behavioural therapy for bulimia nervosa new delivery modes are being examined. Guided Self-Help (GSH) in primary care is potentially valuable in this respect. This research aimed to compare outcomes following GSH delivered by general practitioners (GPs) in the normal course of their practice to a delayed treatment control (DTC) condition, and to examine the maintenance of treatment gains at 3 and 6 months following completion of GSH. Method. Participants were 109 women with full syndrome or sub-threshold bulimia nervosa, randomly allocated to GSH (n=54) and DTC (n=55). The GSH group received direction and support from a GP over a 17-week period while working through the manual in Bulimia Nervosa and Binge-Eating: A Guide to Recovery by P. J. Cooper (1995). GSH and DTC groups were assessed pre-treatment and 1 week following the 17-week intervention or waiting interval. The GSH group was reassessed at 3- and 6-month follow-up. Results. Intention-to-treat analyses at end of treatment revealed significant improvements in bulimic and psychological symptoms in GSH compared with DTC, reduction in mean frequency of binge-eating episodes by 60% in GSH and 6% in DTC, and remission from all binge-eating and compensatory behaviours in 28% of the GSH and 11% of the DTC sample. Treatment gains were maintained at 3- and 6-month follow-up. Conclusion. Outcomes in GSH compare favourably with those of specialist-delivered psychological treatments. These findings are considered in light of the nature of the therapy offered and the primary care context.