Miracle-dz/UniFAR-FTUs huggingface dataset:
Predictors of smoking abstinence following a single-session restructuring intervention with self-hypnosis.

OBJECTIVE This study examined the relation of smoking and medical history, social support, and hypnotizability to outcome of a smoking cessation program. METHOD A consecutive series of 226 smokers referred for the smoking cessation program were treated with a single-session habit restructuring intervention involving self-hypnosis. They were then followed up for 2 years. Total abstinence from smoking after the intervention was the criterion for successful outcome. RESULTS Fifty-two percent of the study group achieved complete smoking abstinence 1 week after the intervention; 23% maintained their abstinence for 2 years. Hypnotizability and having been previously able to quit smoking for at least a month significantly predicted the initiation of abstinence. Hypnotizability and living with a significant other person predicted 2-year maintenance of treatment response. CONCLUSIONS These results, while modest, are superior to those of spontaneous efforts to stop smoking. Furthermore, they suggest that it is possible to predict which patients are most likely and which are least likely to respond to such brief smoking cessation interventions.
Hemolysis following platelet transfusions from ABO‐incompatible donors

Two hemolytic transfusion reactions related to isoagglutinins present in group O platelet concentrates are reported. The first, a severe reaction in a group A 10‐kg patient, resulted from transfusion of 200 ml of plasma containing a hemolytic anti‐A, titer 16, and an IgG anti‐ A, titer of 32,000. In the second case, a group B adult received between 50 and 70 ml of O plasma with a titer (16,000) of anti‐B. This was followed by hemolysis of approximately 40 percent of the patient's red cell volume.
Human Immunodeficiency Virus Type 1 Escapes from RNA Interference-Mediated Inhibition

ABSTRACT Short-term assays have suggested that RNA interference (RNAi) may be a powerful new method for intracellular immunization against human immunodeficiency virus type 1 (HIV-1) infection. However, RNAi has not yet been shown to protect cells against HIV-1 in long-term virus replication assays. We stably introduced vectors expressing small interfering RNAs (siRNAs) directed against the HIV-1 genome into human T cells by retroviral transduction. We report here that an siRNA directed against the viral Nef gene (siRNA-Nef) confers resistance to HIV-1 replication. This block in replication is not absolute, and HIV-1 escape variants that were no longer inhibited by siRNA-Nef appeared after several weeks of culture. These RNAi-resistant viruses contained nucleotide substitutions or deletions in the Nef gene that modified or deleted the siRNA-Nef target sequence. These results demonstrate that efficient inhibition of HIV-1 replication through RNAi is possible in stably transduced cells. Therefore, RNAi could become a realistic gene therapy approach with which to overcome the devastating effect of HIV-1 on the immune system. However, as is known for antiviral drug therapy against HIV-1, antiviral approaches involving RNAi should be used in a combined fashion to prevent the emergence of resistant viruses.
Coexistence of MACC1 and NM23‐H1 dysregulation and tumor budding promise early prognostic evidence for recurrence risk of early‐stage colon cancer

The tumor‐node‐metastasis (TNM) classification, the presence of a mucinous component, and signet ring cells are well‐known criteria for identifying patients at a high risk for recurrence and determining the therapeutic approach for early‐stage colon cancer (eCC). Nevertheless, recurrence can unexpectedly occur in some eCC cases after surgical resection. The aims of the present study were to evaluate the relation of dysregulated MACC1, c‐MET, and NM23‐H1 expression with the histopathological features of tumors in recurrence formation in eCC cases. A total of 100 sporadic eCC patients without poor prognosis factors were evaluated in this study. The relationship between the altered expression of MACC1, c‐MET, and NM23‐H1 and pathological microenvironmental features, including the presence of tumor budding and desmoplasia, were assessed. The primary outcomes, including 5‐year overall survival (OS) and disease‐free survival (DFS), were also measured. Compared with nonrecurrent patients, the expression level of MACC1 was 8.27‐fold higher, and NM23‐H1 was 11.36‐fold lower in patients with recurrence during the 5‐year follow‐up (p = 0.0345 and p = 0.0301, respectively). In addition, the coexistence of high MACC1 and low NM23‐H1 expression and tumor budding was associated with short OS (p < 0.001). We suggest that the combination of reduced NM23‐H1, induced MACC1, and the presence of tumor budding are promising biomarkers for the prediction of recurrence and may aid the stratification of patients with stage II colon cancer for adjuvant chemotherapy.
A simultaneous comparison of three neonatal pain scales during common NICU procedures.

OBJECTIVE This study evaluated neonatal pain scales during procedures commonly performed in a neonatal intensive care unit. DESIGN Evaluated were the Neonatal Infant Pain Scale (NIPS), the Comfort scale, and a new scale known as the Scale for Use in Newborns (SUN). Four procedures were scored: intubation, intravenous catheter insertion, endotracheal tube suctioning, and diaper changes. Scoring was done before, during, and after each procedure. Thirty-three patients were tested during 68 procedures with 1,428 scale scores. RESULTS All scales demonstrated significant changes. In before-versus-during for each procedure, the increase in pain scale score was significant for the NIPS, Comfort scale, and SUN. All three scales also demonstrated a return to baseline (before-vs.-after) for the four procedures, except for the Comfort scale, which remained elevated (p < .05) following diaper change. The NIPS had a significantly larger coefficient of variation (CV, 188% +/- 99%), whereas the Comfort scale and SUN had small CVs (27% +/- 5% and 33% +/- 8%, respectively). In evaluating potential confounding influences, it was found that infants > 2.5 kg on sedative or analgesic medications appeared to have procedure-related accentuation and sustained elevation in scale scores, whereas swaddling seemed to provide little added benefit. CONCLUSIONS The pain scale scores identify changes in an infant's behavior/physiologic state. It is unclear whether these changes are totally "pain specific. " In comparing the three scales, the SUN overall was a preferable tool because of its ease of use, scale symmetry, and scoring consistency.
Robot therapy for functional recovery of the upper limbs: a pilot study on patients after stroke.

OBJECTIVE To verify the possibility of administering robot-aided therapy for the upper limbs in patients after stroke; to evaluate patients' degree of acceptance and compliance with the treatment; to establish if the treatment has an effect on motor impairment and functional outcome. DESIGN Quasi-experimental, uncontrolled study. SUBJECTS Fourteen patients with chronic hemiparesis after stroke. METHODS Patients were treated with a robotic system for the upper limbs (ReoGo; Motorika Medical Ltd, Israel). Subjects performed the following assessment, at the start (T0), at the end of treatment (T1), and at the follow-up performed one month after the end of treatment (T2): Fugl-Meyer test (FM) for upper limbs; strength evaluation; Ashworth scale; visual analogue scale (VAS) for pain; Frenchay Arm test (FAT); Box and Block test (B & B); Functional Independence Measure (FIM); ABILHAND Questionnaire; Timed Up and Go test (TUG); Euro-Quality of Life questionnaire and; a VAS for treatment satisfaction were administered to the subjects. RESULTS Total scores of FM, B&B, FAT and FIM showed a statistically significant improvement from T0 and T1 (FM p < 0.002, B&B p < 0.012, FAT p < 0.023, FIM p < 0.007) and from T0 and T2 (FM p < 0.003, B&B p < 0.011, FAT p < 0.024, FIM p < 0.027). No statistically significant differences were found between evaluations at T1 and T2 (FM p < 0.595, B&B p < 0.491, FAT p < 0.317, FIM p < 0.180). CONCLUSION The sample was capable of completing the treatment and demonstrated good participant satisfaction. This pilot study led to the finding of a clinical improvement and excellent patient compliance. It can be hypothesized that the results are robot-dependent and that they were learned and then maintained. However, the study is limited in that a control group was not used. As such, it is desirable to continue this study with a control group, as well as by designing a prospective longitudinal randomized controlled trial study.
Analysis and Approximation of a Fractional Cahn-Hilliard Equation

We derive a fractional Cahn--Hilliard equation (FCHE) by considering a gradient flow in the negative order Sobolev space $H^{-\alpha}$, $\alpha\in [0,1]$, where the choice $\alpha=1$ corresponds to the classical Cahn--Hilliard equation while the choice $\alpha=0$ recovers the Allen--Cahn equation. The existence of a unique solution is established and it is shown that the equation preserves mass for all positive values of fractional order $\alpha$ and that it indeed reduces the free energy. We then turn to the delicate question of the $L_\infty$ boundedness of the solution and establish an $L_\infty$ bound for the FCHE in the case where the nonlinearity is a quartic polynomial. As a consequence of the estimates, we are able to show that the Fourier--Galerkin method delivers a spectral rate of convergence for the FCHE in the case of a semidiscrete approximation scheme. Finally, we present results obtained using computational simulation of the FCHE for a variety of choices of fractional order $\alpha$. It is.
Image-based three-dimensional finite element modeling approach for upper airway mechanics

The goal of the project is to investigate new computational tools for understanding normal upper airway mechanics and the pathophysiology of obstructive sleep apnea (OSA), a disorder characterized by repetitive pharyngeal collapse and occlusion during sleep. To relate the airway mechanical parameters to anatomy, and explore the roles of primary muscle properties, airway shape and structure, a new computational system for modeling of rat upper airway passive mechanics based on magnetic resonance imaging (MRI) was developed. The model is capable of computing stress and strain distributions under arbitrary loading, and dynamic animation of three-dimensional airway motion
Managing data lakes in big data era: What's a data lake and why has it became popular in data management ecosystem

The concept of a data lake is emerging as a popular way to organize and build the next generation of systems to master new big data challenges, but there are lots of concerns and questions for large enterprises to implement data lakes. The paper discusses the concept of data lakes and shares the author's thoughts and practices of data lakes.
A novel function of RNase P from Escherichia coli: processing of a suppressor tRNA precursor.

The leuX gene of Escherichia coli codes for a suppressor tRNA and forms a single gene operon containing its own promoter and Q‐independent terminator. An analysis of the in vitro processing of leuX precursor revealed that the processing of the 5′ end took place in a single‐step reaction catalysed by RNase P while the 3′ processing involved two successive reactions. The endonucleolytic cleavage activity of the 3′ precursor sequence was found to copurify with RNase P. Heat inactivation of thermosensitive RNase P from two independent E. coli mutants abolished the cleavage activity of both the 5′ and 3′ ends. These results altogether suggest that RNase P carries the activity of 3′ end cleavage as well as that of 5′ processing. In the presence of Mg2+ alone, the leuX precursor was found to be self‐cleaved at a site approximately 13 nt inside from the 5′ end of mature tRNA. The self‐cleaved precursor tRNA was no longer processed by the 3′ endonuclease, suggesting that the 3′ endonuclease recognizes a specific conformation of the precursor tRNA for action.
Marital Interaction in Middle and Old Age: A Predictor of Marital Satisfaction?

Many studies point out the importance of marital satisfaction for well-being. However, although being married is still the norm in middle and old age, research on the determinants of marital satisfaction has neglected long-term marriages. While research on short-term marriages mainly focuses on partner fit (e.g., in personality traits and socio-economic status), marital interactions should be more important for marital satisfaction in long-term marriages. In this article we examine the role of both stable, dispositional factors and of marital interaction in predicting marital satisfaction in long-term marriages in middle and old age. With data from 588 married women and men in middle and old age who participated in the Interdisciplinary Longitudinal Study of Adult Development (ILSE), we examined age and gender differences in the role of socio-economic status, personality, and marital interaction as predictors of marital satisfaction. Results showed a) that socio-economic factors and personality played a minor role in predicting marital satisfaction, and b) that a high quality of dyadic interaction was particularly important for the marital satisfaction of women. The implications of these findings for future research and interventions on marital interaction in middle and old age are discussed.
Selective Decline in Information Processing in Subgroups of Multiple Sclerosis: An 8-Year Longitudinal Study

Multiple sclerosis (MS) is an inflammatory and degenerative disease of the central nervous system (CNS) that causes white matter and cortical lesions over many years. The CNS is selectively affected by the disease with a great variety of symptoms between patients. In this study, we describe the impact on various aspects of cognition over an 8-year follow-up period in 31 consecutive MS patients subgrouped as relapsing remitting (RR) MS, secondary progressive (SP) MS, and primary progressive (PP) MS. Results showed a differential pattern of cognitive decline already at baseline in speed of information processing. During the follow-up, a pronounced decline occurred in speed of information processing, finger-motor speed, copying geometrical designs, episodic memory, and visuospatial short-term memory. A striking difference was observed between a marked decline in visual reaction time, whereas no significant change was seen in auditory reaction time. In contrast, there was no time-related decline in verbal abilities. However, an initial marked cognitive impairment predicted further cognitive decline over the 8-year follow-up. Information-processing tests were found to be an especially strong predictor of long-term cognitive decline. In addition, high EDSS score at follow-up was associated with decline in information processes. Results also showed that SP-MS patients deteriorated significantly more than the other two groups, particularly in visual compared to auditory information processing. To conclude, cognitive decline appeared particularly in SP-MS patients and in visual information processing.
Procalcitonin in Children with Escherichia coli O157:H7 Associated Hemolytic Uremic Syndrome

Shiga toxin producing Escherichia coli (STEC) are noninvasive enteric pathogens that may cause hemorrhagic colitis (HC) and diarrhea-associated hemolytic uremic syndrome (D+ HUS). We hypothesized that development of D+ HUS is associated with increased serum procalcitonin (PCT) levels. PCT was measured by an immunoluminometric assay in 113 patients. Concentrations of PCT were different in normal controls, disease control groups (rotavirus enteritis, HC due to non-STEC pathogens, chronic renal failure), and children with uncomplicated O157:H7 HC or D+ HUS. Children with D+ HUS showed higher PCT levels than those with uncomplicated O157:H7 HC, and increased concentrations were noted in cases requiring peritoneal dialysis. Severely increased concentrations were observed in children with D+ HUS on d 5 or 6 after the onset of enteritis, whereas serial measurements in those with uncomplicated O157:H7 HC remained within the normal range throughout the first week of illness. PCT was correlated with serum concentrations of lipopolysaccharide (LPS)-binding protein and serum levels of alanine aminotransferase. Using two separate sets of real-time PCR primers, we were unable to detect elevated PCT mRNA transcripts in nonadherent undifferentiated (monocytic) or differentiated (macrophage-like) THP-1 cells stimulated with purified Shiga toxin-1 and/or LPS. Our data show that serum levels of PCT are associated with the severity of illness in children with D+ HUS. Further studies are needed to determine the role of PCT in the pathogenesis of thrombotic microangiopathy associated to childhood D+ HUS.
Optimal design of electrical drive and power Converter for hybrid electric powertrain

A modeling and design method of electrical drive and power converter applied to a powertrain for a heavy duty Series-Parallel Hybrid Electric Vehicle (HEV) is described in this paper. A Surface-Mounted Permanent Magnet Synchronous Motor (SMPMSM) is considered and the motor performances are validated with Finite Element Method (FEM) simulations. Moreover, the authors investigated an optimization strategy focused on multi-criteria optimization, taking into account electrical motor modeling, electromechanical behavior modeling and the overall losses modeling including motor losses and converter losses. The optimization of the powertrain is achieved realizing a compromise between the efficiency and the motor weight minimization.
Effects of COL8A1 on the proliferation of muscle‐derived satellite cells

Collagen type VIII alpha 1 chain (COL8A1) is a component of the extracellular matrix. Our previous studies suggested that COL8A1 is associated with the proliferation of muscle‐derived satellite cells (MDSCs). Additionally, it has been demonstrated that COL8A1 promotes the proliferation of smooth muscle cells and liver cancer cells. Therefore, we predicted that COL8A1 is associated with the proliferation of bovine MDSCs, which have potential applications in research. In this study, we constructed vectors to activate and repress COL8A1 in bovine MDSCs using the CRISPR/Cas9 technique and determined the effects of COL8A1 modulation by EdU labeling, Western blotting, and dual‐luciferase reporter assays. The results showed that activation of COL8A1 increased the number of EdU‐positive cells and expression of the proliferation markers cyclin B1 (CCNB1) and P‐AKT. The expression of P‐Akt was unchanged after addition of LY294002 (a protein kinase inhibitor capable of blocking the signal transduction pathway of the phosphoinositide 3‐kinase). In contrast, repression of COL8A1 reduced the number of EdU‐positive cells and expression of CCNB1 and P‐AKT. We also observed upregulation and downregulation of COL8A1 following the overexpression and repression of EGR1, respectively. The dual‐luciferase reporter assay revealed that EGR1 regulates the promoter activity of COL8A1. To our knowledge, this is the first study demonstrating that EGR1 positively regulates the expression of COL8A1, which in turn promotes the proliferation of bovine MDSCs via the PI3 K/AKT signaling pathway.
The design of zero-IF receiver of UHF RFID reader

This paper have elaborated the technology and application of RFID briefly, and analyzed zero intermediate-frequency receiver's theory and several inevitable key questions when used in the system. For example, the receive scotoma which produces by the label position change, signal direct-current displacement and so on. The paper proposes the solution and has carried on the actual electric circuit's test and the confirmation.
Retention of the duplicated cellular retinoic acid‐binding protein 1 genes (crabp1a and crabp1b) in the zebrafish genome by subfunctionalization of tissue‐specific expression

The cellular retinoic acid‐binding protein type I (CRABPI) is encoded by a single gene in mammals. We have characterized two crabp1 genes in zebrafish, designated crabp1a and crabp1b. These two crabp1 genes share the same gene structure as the mammalian CRABP1 genes and encode proteins that show the highest amino acid sequence identity to mammalian CRABPIs. The zebrafish crabp1a and crabp1b were assigned to linkage groups 25 and 7, respectively. Both linkage groups show conserved syntenies to a segment of the human chromosome 15 harboring the CRABP1 locus. Phylogenetic analysis suggests that the zebrafish crabp1a and crabp1b are orthologs of the mammalian CRABP1 genes that likely arose from a teleost fish lineage‐specific genome duplication. Embryonic whole mount in situ hybridization detected zebrafish crabp1b transcripts in the posterior hindbrain and spinal cord from early stages of embryogenesis. crabp1a mRNA was detected in the forebrain and midbrain at later developmental stages. In adult zebrafish, crabp1a mRNA was localized to the optic tectum, whereas crabp1b mRNA was detected in several tissues by RT‐PCR but not by tissue section in situ hybridization. The differential and complementary expression patterns of the zebrafish crabp1a and crabp1b genes imply that subfunctionalization may be the mechanism for the retention of both crabp1 duplicated genes in the zebrafish genome.
Cyclic-di-GMP-Mediated Repression of Swarming Motility by Pseudomonas aeruginosa: the pilY1 Gene and Its Impact on Surface-Associated Behaviors

ABSTRACT The intracellular signaling molecule cyclic-di-GMP (c-di-GMP) has been shown to influence surface-associated behaviors of Pseudomonas aeruginosa, including biofilm formation and swarming motility. Previously, we reported a role for the bifA gene in the inverse regulation of biofilm formation and swarming motility. The bifA gene encodes a c-di-GMP-degrading phosphodiesterase (PDE), and the ΔbifA mutant exhibits increased cellular pools of c-di-GMP, forms hyperbiofilms, and is unable to swarm. In this study, we isolated suppressors of the ΔbifA swarming defect. Strains with mutations in the pilY1 gene, but not in the pilin subunit pilA gene, show robust suppression of the swarming defect of the ΔbifA mutant, as well as its hyperbiofilm phenotype. Despite the ability of the pilY1 mutation to suppress all the c-di-GMP-related phenotypes, the global pools of c-di-GMP are not detectably altered in the ΔbifA ΔpilY1 mutant relative to the ΔbifA single mutant. We also show that enhanced expression of the pilY1 gene inhibits swarming motility, and we identify residues in the putative VWA domain of PilY1 that are important for this phenotype. Furthermore, swarming repression by PilY1 specifically requires the diguanylate cyclase (DGC) SadC, and epistasis analysis indicates that PilY1 functions upstream of SadC. Our data indicate that PilY1 participates in multiple surface behaviors of P. aeruginosa, and we propose that PilY1 may act via regulation of SadC DGC activity but independently of altering global c-di-GMP levels.