Miracle-dz/UniFAR-FTUs huggingface dataset:
Swimming muscles power suction feeding in largemouth bass

Significance Over one-half of all vertebrate species are ray-finned fishes. Across this extraordinary diversity, the most common feeding mode is suction feeding: rapid expansion of the mouth to suck in water and food. Here, we find that the power required for suction expansion is generated primarily by the axial swimming muscles. Rather than being restricted to the low power capacity of the small cranial muscles, suction-feeding fishes have co-opted the massive swimming muscles for this powerful feeding behavior. Therefore, the evolution of axial muscles in ray-finned fishes should now be considered in the context of feeding as well as locomotion, changing our perspective on musculoskeletal form and function in over 30,000 species. Most aquatic vertebrates use suction to capture food, relying on rapid expansion of the mouth cavity to accelerate water and food into the mouth. In ray-finned fishes, mouth expansion is both fast and forceful, and therefore requires considerable power. However, the cranial muscles of these fishes are relatively small and may not be able to produce enough power for suction expansion. The axial swimming muscles of these fishes also attach to the feeding apparatus and have the potential to generate mouth expansion. Because of their large size, these axial muscles could contribute substantial power to suction feeding. To determine whether suction feeding is powered primarily by axial muscles, we measured the power required for suction expansion in largemouth bass and compared it to the power capacities of the axial and cranial muscles. Using X-ray reconstruction of moving morphology (XROMM), we generated 3D animations of the mouth skeleton and created a dynamic digital endocast to measure the rate of mouth volume expansion. This time-resolved expansion rate was combined with intraoral pressure recordings to calculate the instantaneous power required for suction feeding. Peak expansion powers for all but the weakest strikes far exceeded the maximum power capacity of the cranial muscles. The axial muscles did not merely contribute but were the primary source of suction expansion power and generated up to 95% of peak expansion power. The recruitment of axial muscle power may have been crucial for the evolution of high-power suction feeding in ray-finned fishes.
Cellular softening mediates leukocyte demargination and trafficking, thereby increasing clinical blood counts

Significance Clinical hematologists have long known that antiinflammatory glucocorticoids such as dexamethasone and blood pressure-supporting catecholamines such as epinephrine cause leukocytes to demarginate from the vascular wall and microvasculature into the main circulation, significantly elevating the effective white blood cell count. Canonically, this has been attributed to down-regulation of adhesion molecules such as selectins, but we show that a purely mechanical phenomenon caused by leukocyte softening plays a major role as well. Our work provides an answer to an old hematological problem and reveals a mechanism in which the immune system simply alters leukocyte stiffness to regulate leukocyte trafficking. This has clinically relevant implications for the inflammatory process overall as well as for hematopoietic stem cell mobilization and homing. Leukocytes normally marginate toward the vascular wall in large vessels and within the microvasculature. Reversal of this process, leukocyte demargination, leads to substantial increases in the clinical white blood cell and granulocyte count and is a well-documented effect of glucocorticoid and catecholamine hormones, although the underlying mechanisms remain unclear. Here we show that alterations in granulocyte mechanical properties are the driving force behind glucocorticoid- and catecholamine-induced demargination. First, we found that the proportions of granulocytes from healthy human subjects that traversed and demarginated from microfluidic models of capillary beds and veins, respectively, increased after the subjects ingested glucocorticoids. Also, we show that glucocorticoid and catecholamine exposure reorganizes cellular cortical actin, significantly reducing granulocyte stiffness, as measured with atomic force microscopy. Furthermore, using simple kinetic theory computational modeling, we found that this reduction in stiffness alone is sufficient to cause granulocyte demargination. Taken together, our findings reveal a biomechanical answer to an old hematologic question regarding how glucocorticoids and catecholamines cause leukocyte demargination. In addition, in a broader sense, we have discovered a temporally and energetically efficient mechanism in which the innate immune system can simply alter leukocyte stiffness to fine tune margination/demargination and therefore leukocyte trafficking in general. These observations have broad clinically relevant implications for the inflammatory process overall as well as hematopoietic stem cell mobilization and homing.
Is intrinsic asthma synonymous with infection?

Rackemann described the ‘intrinsic asthma’ population over 50 years ago as a unique subgroup that was characterized by onset of progressive loss of lung function beginning later in life, possibly after a respiratory infection. It has also been associated with a female predominance, aspirin‐sensitive bronchospasm, and nasal polyposis. While the aetiology is not understood, we propose that persistent respiratory infections play a central role in the development of intrinsic asthma.
Chemical, experimental, and morphological evidence for diagenetically altered melanin in exceptionally preserved fossils

Significance Melanin is a widespread pigment that provides black to reddish brown hues to organisms. Recent evidence has shown that melanin is retained in exceptionally preserved fossils, including feathered dinosaurs, allowing the reconstruction of ancient color patterns. However, little is known about the chemical preservation of melanin or its distribution in the fossil record. Here, we show that melanin is preserved in a number of soft-bodied fossils, but its burial under high pressure and temperature for millions of years alters its original chemistry. The widespread occurrence of melanin substantiates the applicability of reconstructing aspects of original color patterns and allows us to dismiss the alternative suggestion that these structures are microbial in origin. In living organisms, color patterns, behavior, and ecology are closely linked. Thus, detection of fossil pigments may permit inferences about important aspects of ancient animal ecology and evolution. Melanin-bearing melanosomes were suggested to preserve as organic residues in exceptionally preserved fossils, retaining distinct morphology that is associated with aspects of original color patterns. Nevertheless, these oblong and spherical structures have also been identified as fossilized bacteria. To date, chemical studies have not directly considered the effects of diagenesis on melanin preservation, and how this may influence its identification. Here we use time-of-flight secondary ion mass spectrometry to identify and chemically characterize melanin in a diverse sample of previously unstudied extant and fossil taxa, including fossils with notably different diagenetic histories and geologic ages. We document signatures consistent with melanin preservation in fossils ranging from feathers, to mammals, to amphibians. Using principal component analyses, we characterize putative mixtures of eumelanin and phaeomelanin in both fossil and extant samples. Surprisingly, both extant and fossil amphibians generally exhibit melanosomes with a mixed eumelanin/phaeomelanin composition rather than pure eumelanin, as assumed previously. We argue that experimental maturation of modern melanin samples replicates diagenetic chemical alteration of melanin observed in fossils. This refutes the hypothesis that such fossil microbodies could be bacteria, and demonstrates that melanin is widely responsible for the organic soft tissue outlines in vertebrates found at exceptional fossil localities, thus allowing for the reconstruction of certain aspects of original pigment patterns.
Developments in allergen‐specific immunotherapy: from allergen extracts to allergy vaccines bypassing allergen‐specific immunoglobulin E and T cell reactivity

Allergen‐specific immunotherapy (SIT) is the only specific and disease‐modifying approach for the treatment of allergy but several disadvantages have limited its broad applicability. We argue that the majority of the possible disadvantages of SIT such as unwanted effects, poor efficacy and specificity as well as inconvenient application are related to the poor quality of natural allergen extracts, which are the active ingredients of all currently available allergy vaccines. Because of the progress made in the field of molecular allergen characterization, new allergy vaccines based on recombinant allergens, recombinant hypoallergenic allergen derivatives and allergen‐derived T cell peptides have entered clinical testing and hold promise to reduce the side‐effects and to increase the specificity as well as the efficacy of SIT. Here, we present a refined immunotherapy concept, which is based on the use of peptides derived from allergen surfaces that exhibit reduced, allergen‐specific IgE as well as T cell reactivity. These peptides when fused to non‐allergenic carriers give rise to allergen‐specific protective IgG responses with T cell help from a non‐allergenic carrier molecule. We summarize the experimental data demonstrating that such peptide vaccines can bypass allergen‐specific IgE as well as T cell activation and may be administered at high doses without IgE‐ and T cell‐mediated side‐effects. Should these peptide vaccines prove efficacious and safe in clinical trials, it may become possible to develop convenient, safe and broadly applicable forms of SIT as true alternatives to symptomatic, drug‐based allergy treatment.
The natural history of light smokers: a population-based cohort study.

INTRODUCTION Among cigarette smokers, lower levels of consumption, defined as smoking fewer cigarettes per day (CPD) or not smoking daily, are becoming more common. The relationship between cigarette consumption and smoking frequency (daily or nondaily) is not well characterized, and the natural history of light smoking (defined here as smoking < or =10 CPD) is poorly understood. METHODS We assessed changes in CPD and smoking frequency over time among light smokers (< or =10 CPD) and very light smokers (< or =5 CPD), using a population-based longitudinal survey of 3,083 adult smokers in Massachusetts who were interviewed three times over a 4-year follow-up period (in 2000-2001, 2002-2003, and 2005-2006). We used logistic regression to identify factors associated with light smokers' progression to heavier smoking or smoking reduction/quitting. RESULTS Seventy percent of very light smokers were nondaily smokers. Very light nondaily smokers differed from very light daily smokers by younger age, higher socioeconomic status, a social smoking pattern, later smoking initiation, less evidence of nicotine addiction, and more recent and planned cessation efforts. Very light nondaily smokers and smokers consuming 6-10 CPD were more likely to remain in the same smoking category and were less likely to increase consumption than were very light daily smokers. Factors independently associated with increasing consumption among very light smokers were smoking daily, nicotine dependence, White ethnicity, social smoking, and having more friends who smoked; among smokers consuming 6-10 CPD, male gender and lack of quitting self-efficacy were associated with increasing consumption. CONCLUSION Our findings indicate that most light smoking is not a gateway to heavier smoking.
HIV/AIDS and HCV in prisons: A select annotated bibliography (part 2)

The rates of HIV infection among prisoners in most countries are significantly higher than in the general population. HCV seroprevalence rates are even higher. While most prisoners living with HIV or AIDS and/or HCV contract their infection(s) outside the institutions before imprisonment, there is evidence that the risk of being infected in prison, in particular through sharing of contaminated injecting equipment and through unprotected sex, is great. Outbreaks of HIV infection have been documented in a number of countries. Since the early 1990s, various countries have introduced HIV and, to a lesser extent, HCV prevention programmes in prisons. Part 2 of the select annotated bibliography on HIV/AIDS and HCV in prisons contains selected “essential” articles and reports that provide information about (1) prevalence of HIV, HCV, and risk behaviours in prisons; (2) transmission of HIV and HCV in prisons; and (3) measures aimed at preventing HIV and HCV infection in prisons: education, voluntary testing and c.
Laboratory professionals in Africa : the backbone of quality diagnostics

Laboratories have historically been under supported in developing country health systems resulting in poor quality diagnosis and inadequate disease surveillance. Laboratory professionals are predominantly male with relatively limited female labor participation, with potential gender based barriers to advancement. The focus on communicable diseases has meant that funding for broader public health laboratory services has been relatively neglected. In this paper the authors present a number of strategies to address these problems based on the outcomes from a literature review and case studies conducted in four African countries. Improved registration and human resource planning are required to establish the scale of the problem and to develop country specific strategies to address skills shortages. More high quality pre-service training is needed to supply the service with suitably skilled professional staff to address the current deficit. Innovative in-service training is essential to maintain competence and collaboration is required with the private sector to utilize their expertise. A clear career structure with transparent promotional opportunities is required to recruit and retain staff in the public sector. The establishment of suitable work environments and regulatory and representative bodies will also support recruitment and retention as well as enhance quality. It is also clear that this cadre has been underrepresented in human resources for health research and more activity in this area will lead to greater understanding of the problems and provide more potential solutions.
Production diversity and dietary diversity in smallholder farm households

Significance Given that hunger and malnutrition are still widespread problems in many developing countries, the question of how to make agriculture and food systems more nutrition-sensitive is of high relevance for research and policy. Many of the undernourished people in Africa and Asia are small-scale subsistence farmers. Diversifying production on these farms is often perceived as a promising strategy to improve dietary quality and diversity. This hypothesis is tested with data from smallholder farm households in Indonesia, Kenya, Ethiopia, and Malawi. Higher farm production diversity significantly contributes to dietary diversity in some situations, but not in all. Improving small farmers’ access to markets seems to be a more effective strategy to improve nutrition than promoting production diversity on subsistence farms. Undernutrition and micronutrient malnutrition remain problems of significant magnitude in large parts of the developing world. Improved nutrition requires not only better access to food for poor population segments, but also higher dietary quality and diversity. Because many of the poor and undernourished people are smallholder farmers, diversifying production on these smallholder farms is widely perceived as a useful approach to improve dietary diversity. However, empirical evidence on the link between production and consumption diversity is scarce. Here, this issue is addressed with household-level data from Indonesia, Kenya, Ethiopia, and Malawi. Regression models show that on-farm production diversity is positively associated with dietary diversity in some situations, but not in all. When production diversity is already high, the association is not significant or even turns negative, because of foregone income benefits from specialization. Analysis of other factors reveals that market access has positive effects on dietary diversity, which are larger than those of increased production diversity. Market transactions also tend to reduce the role of farm diversity for household nutrition. These results suggest that increasing on-farm diversity is not always the most effective way to improve dietary diversity in smallholder households and should not be considered a goal in itself. Additional research is needed to better understand how agriculture and food systems can be made more nutrition-sensitive in particular situations.
Chemically Robust Ambipolar Organic Transistor Array Directly Patterned by Photolithography

Organic ambipolar transistor arrays for chemical sensors are prepared on a flexible plastic substrate with a bottom-gate bottom-contact configuration to minimize the damage to the organic semiconductors, for the first time, using a photolithographically patternable polymer semiconductor. Well-balanced ambipolar charge transport is achieved by introducing graphene electrodes because of the reduced contact resistance and energetic barrier for electron transport.
Thinking from God’s perspective decreases biased valuation of the life of a nonbeliever

Significance Religious belief is often seen as a key cause of human conflict because it is said to promote preferential treatment of adherents and to harden group boundaries. Here, we examined a critical aspect of this link in the context of the Israeli–Palestinian conflict, a multigenerational violent conflict with significant religious aspects. We find that although Muslim Palestinian participants valued Palestinian over Jewish Israeli lives when making difficult moral choices, they believed that Allah preferred them to make moral decisions that valued the lives of Palestinians and Jewish Israelis more equally. Beliefs about God may promote more equal valuation of human life regardless of religious identity, encouraging application of universal moral rules to believers and nonbelievers alike. Religious belief is often thought to motivate violence because it is said to promote norms that encourage tribalism and the devaluing of the lives of nonbelievers. If true, this should be visible in the multigenerational violent conflict between Palestinians and Israelis which is marked by a religious divide. We conducted experiments with a representative sample of Muslim Palestinian youth (n = 555), examining whether thinking from the perspective of Allah (God), who is the ultimate arbitrator of religious belief, changes the relative value of Jewish Israelis’ lives (compared with Palestinian lives). Participants were presented with variants of the classic “trolley dilemma,” in the form of stories where a man can be killed to save the lives of five children who were either Jewish Israeli or Palestinian. They responded from their own perspective and from the perspective of Allah. We find that whereas a large proportion of participants were more likely to endorse saving Palestinian children than saving Jewish Israeli children, this proportion decreased when thinking from the perspective of Allah. This finding raises the possibility that beliefs about God can mitigate bias against other groups and reduce barriers to peace.
Effects of acute psychosocial stress on cigarette craving and smoking.

INTRODUCTION Stress is thought to influence use of drugs, including cigarette smoking, but the mechanisms by which it does so are not clear. In this study, we investigated the effects of acute psychosocial stress on cigarette craving, the subjective effects of smoking, and smoking behavior in daily smokers. METHODS Healthy male and female smokers participated in two experimental sessions in which they were exposed to the Trier Social Stress Test or a nonstressful control task. For 2 hr after each task, they had repeated opportunities to either smoke or earn money. Physiological (heart rate, cortisol, and alpha-amylase) and subjective (anxiety and desire to smoke) measures were obtained before and after the tasks and after each smoking opportunity. RESULTS Stress significantly increased cigarette craving but it did not increase smoking. When individual differences in nicotine dependence were taken into account, stress influenced CO boost and pleasure from smoking the first cigarette. DISCUSSION Our results support previous evidence that acute psychosocial stress increases smoking desire.
Videotape review versus peer review for evaluating communication skills used by undergraduate dental students during a child’s initial dental consultation

Aim: The primary aim was to compare undergraduate dental students’ preferences of videotape and observer peer review in evaluating undergraduate dental students’ communication skills during child initial consultation. The Secondary Research aims were 1. To examine the intra- and inter-observer agreement of an established Paediatric Consultation Scale (PCAT) over a one-week period for evaluating a dental student consultation with a child-patient and their parent. 2. To assess the correlation between parental opinions concerning the quality of the consultation using a combination of the dentist-patient interaction tool and students’ opinions. Methods: A pilot study of (42) undergraduate dental students from Leeds Dental Institute together with 21 children and their parents participated in this study. Undergraduate dental students acted as either peer observers or consulting dentists. The consulting dentists conducted a first clinical visit appointment for the child and parent; the peer observers observed the consultation and recorded observations using a Paediatric Consultation Assessment Tool (PCAT). Following the completion of the consultation, the consulting dentist was asked to complete the PCAT scale to critique their own performance throughout the consultation. Furthermore, the parents were asked for their opinions of the consultation using the dentist-patient interaction tool. Finally, the consultation was videotaped by the researcher. One week later, the same peer observer and consulting dentists reviewed the videotape with the researcher. They completed a further PCAT scale, as well as a qualitative questionnaire, with the objective to explore their feelings in terms of which method they preferred, video-tape review or peer review. Results: Using framework analysis the qualitative questionnaire was evaluated and showed that the students’ prefer video review feedback. Cohen’s Kappa was used to test the agreement between students and showed slight agreement. Spearman’s rank correlation was used to test the correlation between the parental satisfaction and the students’ opinion and showed week correlation. Conclusion: The under graduate students preferred the video review over the peer review. However, the students failed to agree on the quality of the consultation and none of their opinion represented the parental opinion.