Miracle-dz/UniFAR-FTUs huggingface dataset:
Régulation traductionnelle en réponse à la fécondation et en conditions perturbées dans l’embryon d’oursin

La traduction est une etape critique de la regulation de l'expression des genes. Chez l'oursin, la fecondation induit une augmentation de la synthese proteique, qui depend essentiellement de la traduction d'ARN messagers maternels. Cette synthese proteique est indispensable au deroulement des cycles cellulaires du developpement precoce. Le developpement embryonnaire de l'oursin constitue ainsi un modele de choix pour etudier la regulation de la traduction. Dans le cadre de cette these, le controle de la traduction a ete etudie chez l'oursin dans deux situations : le contexte physiologique de la fecondation et le contexte de l'induction de l'apoptose. Nous nous sommes interroges d'abord sur les mecanismes regulateurs impliques dans la synthese proteique apres fecondation. L'une des etapes limitantes de la traduction est l'initiation. Dans ce cadre, le facteur d'initiation eIF2 joue un role cle. eIF2 est responsable de l'apport de la methionine initiatrice au niveau du ribosome. Lorsque la sous-unite alpha d'eIF2 est phosphorylee, la synthese proteique globale est inhibee et la traduction selective de certains ARNm est stimulee. Dans les ovules non fecondes d'oursin, eIF2alpha est physiologiquement phosphoryle et la fecondation provoque sa dephosphorylation. En micro-injectant dans les ovules non fecondes un variant d'eIF2alpha mimant l'etat phosphoryle, nous avons montre que la dephosphorylation d'eIF2alpha est necessaire pour la premiere division mitotique chez l'oursin. Nous nous sommes interesses au lien entre la phosphorylation d'eIF2alpha et l'induction de l'apoptose chez l'oursin. En effet, la traduction d'ARNm codant pour des proteines pro- ou anti- apoptotiques influence directement la survie des cellules. L'embryon d'oursin possede la machinerie necessaire pour le declenchement de l'apoptose, apres induction par l'agent genotoxique MMS. Le traitement au MMS des embryons provoque la phosphorylation d'eIF2alpha. Dans cette situation, nous avons trouve que la kinase GCN2 est impliquee dans la phosphorylation d'eIF2alpha. En fin, dans le but d'etudier comment la machinerie traductionnelle module le recrutement polysomal, nous avons analyse le traductome en reponse a la fecondation et apres le traitement au MMS. Nous avons effectue une approche de sequencage a haut-debit des transcrits purifies par gradients de polysomes. L'analyse de ces transcrits nous permettra d'apprehender le reseau des genes regules au niveau traductionnel lors de la fecondation et de l'induction de l'apoptose dans les embryons d'oursin.
Traffic Volume Forecasting Model by using Elasticity Method and Exponential Smooth Model for Main National Roads of Rwanda

The accuracy in traffic forecasting helps the planners to have a good plan about the highways as the forecasting volume has been recognized as one of the factors that involve in the analysis of highway planning, reconstruction, new construction as well as maintenance. This paper presents the models to forecast the selected main roads in Rwanda by comparing two methods: elastic method and exponential smooth model. This study shows that the traffic forecasting by using exponential smooth model can overestimate and doesn’t consider the demographic as well as economic variables and shows that the elastic model is a best method to estimate and forecast the traffic volume as it combines the demographic data as well as the economic ones but the rate is slow. The best estimate is the combination of the two methods.
What is retained about common ground? Distinct effects of linguistic and visual co-presence

Common ground can be mutually established between conversational partners in several ways. We examined whether the modality (visual or linguistic) with which speakers share information with their conversational partners results in memory traces that affect subsequent references addressed to a particular partner. In 32 triads, directors arranged a set of tangram cards with one matcher and then with another, but in different modalities, sharing some cards only linguistically (by describing cards the matcher couldn't see), some only visually (by silently showing them), some both linguistically and visually, and others not at all. Then directors arranged the cards again in separate rounds with each matcher. The modality with which they previously established common ground about a particular card with a particular matcher (e.g., linguistically with one partner and visually with the other) affected subsequent referring: References to cards previously shared only visually included more idea units, words, and reconceptualizations than those shared only linguistically, which in turn included more idea units, words, and reconceptualizations than those shared both linguistically and visually. Moreover, speakers were able to tailor references to the same card appropriately to the distinct modality shared with each addressee. Such gradient, partner-specific adaptation during re-referring suggests that memory encodes rich-enough representations of multimodal shared experiences to effectively cue relevant constraints about the perceptual conditions under which speakers and addressees establish common ground.
Parasitoids incidence and diversity on maize stem borers Sesamia nonagrioides Lefèbvre and Ostrinia nubilalis Hübner in NW Spain

The most important insect pests on maize in Spain are the pink stem borer S. nonagrioides (Lefebvre) and the European corn borer O. nubilalis (Hubner). Parasitoid decree of control on stem borers and other maize pests in Spain is not known. The aim of this research was to evaluate the incidence and diversity of parasitoids on maize stem borers in the province of Pontevedra (NW Spain). Samplings of hybrid field maize plants (DMB-1570) were taken in four locations, from March 1999 to November 2000. Plants were dissected and stem borer overwintering and non-overwintering larvae were collected in separated plastic boxes. Larvae of Mythimua spp. and Helicoverpa armigera were also collected. Larvae were reared in the insectary and adult parasitoids obtained from them were determined. Diversity of parasitoids was in general very low. The most frequently found species on non-over-wintering larvae was Lydella thompsoni (Herting) (Diptera: Tachinidae). Differences in percentage of parasitism (PP) between O. nubilatis and S. nonagrioides were significant. Lydella thompsoni was also the dominant parasitoid on overwintering larvae. Its PP was higher on O. nubilalis than on S. nonagrioides. The correlation between number of stem borer per plain and percentage of parasitised larvae was not significant, neither for S. nonagrioides nor for O. nubilalis. Shannon-Wiener index as a measure of parasitoid diversity was similar in the different hosts. In conclusion, a very low parasitoid natural control on maize borers, in hybrid corn crops grown at the province of Pontevedra was found. Low numbers of parasitoids and low percentage of parasitism in the studied area make difficult to draw definitive conclusions. Stem borers seem to be protected from parasitoids by several circumstances. specifically the development of their immature stages within refuges (the stalks), but other factors probably would contribute to the situation.
Etude du dialogue hote / bacteries lactiques du yaourt chez des rats gnotobiotiques

L'amelioration de la digestion de lactose est une allegation "sante" liee aux ferments viviants du yaourt : Streptococcus thermophilus (S. thermophilus) et Lactobacillus delbrueckii ssp. bulgaricus (L. bulgaricus) validee par l'EFSA en 2010. La physiologie de S. thermophilus et de L. bulgaricus est connue dans le lait et particulierement le yaourt, alors qu'elle n'a ete que peu etudiee dans le tractus digestif (TD). Mon travail de these est base sur l'hypothese de travail suivante : l'utilisation de modeles animaux gnotobiotiques permet de mieux connaitre la physiologie des bacteries lactiques et de proposer des mecanismes d'action de leurs effets "sante". La strategie a donc ete d'obtenir des animaux mono-associes avec chacune des deux bacteries du yaourt ou les deux en meme temps. Les principaux resultats obtenus sont : 1/ S. thermophilus colonise le TD en s'adaptant progressivement a l'environnement colique et y induit une glycolyse massive et une production de lactate. La glycolyse est la signature majeure de S. thermophilus dans le TD et le lactate pourrait etre est la molecule "signal" qui induit une reponse chez l'hote par une augmentation des transporteurs de mono-carboxylates (SLC16A1 et SLC5A8) et d'une proteine impliquee dans l'arret du cycle cellulaire p27kip1. 2/ L'apport de lactose stimule la colonisation du TD, la glycolyse ainsi que la production de L-lactate par S. thermophilus in vivo. 3/ Contrairement a ce qui est observe pour S. thermophilus, L. bulgaricus ne s'implante pas en absence de lactose. Quand les deux bacteries sont en co-culture, S. thermophilus est toujours avantage numeriquement par rapport a L. bulgaricus aussi bien in vitro qu' in vivo. Au niveau nutritionnel, tous nos resultats sont coherents avec les allegations "sante" du yaourt avec un effet prebiotique du lactose. L'etude d'animaux gnotobiotiques a permis de proposer des nouvelles voies de regulation du metabolisme des sucres de bacteries lactiques et de nouvelles voies moleculaires (via le lactate) par lesquelles des bacteries lactiques et de nouvelles voies moleculaires (via le lactate) par lesquelles des bacteries lactiques pourraient influencer la physiologie de l'hote.
Investigation of cytotoxic effects of some novel synthesized iminothiazolidinone derivatives on HeLa cell line (CCL2)

In medicinal chemistry, the thiazolidinones are a practical framework which can be leaned as a pharmacophore in a large diversity of biologically active compounds. Furthermore, they build up the base of antibacterials, anti-convulsant, anti-tumorals, antivirals, anti-diabetic, anti-inflammatory, anti- HIV compounds in many other therapeutic agents. In this study, the cytotoxic effects of some novel synthesized iminothiazolidinone derivatives (Compound A-E) on HeLa (3) cell line (CCL2) which arising from human cervical carcinoma were studied. Accordingly, kinetics parameters of proliferation rate, mitotic and the labeling index were determined upon the application of the iminothiazolidinone derivatives. 1 x 10 -6 , 5 x 10 -6 , 10 x 10 -6 M concentrations of the derivatives were implemented for 72 hours to find out the optimum concentrations, and the result was explicated by reproduction rate analysis. Parameters of the mitotic and labeling index of the cells which were treated with the optimum concentrations of the uniquely synthesized iminothiazolidinone derivatives for 0-72 hours were calculated. The results indicated that the tested compounds caused a remarkable decrease in the propagation of HeLa cell cultures and the 10 x 10 -6 M concentration was found to be the most effective concentration of the iminothiazolidinone derivatives regarding reducing the reproduction rate. Drugs can be obtained from these derivatives will offer a promising treatment modality in cervix carcinoma in the future.
Caracterización productiva de las variedades del cerdo ibérico, II: estudio preliminar del peso al nacimiento y pesos a las primeras edades

Se estudia el comportamiento de seis variedades del cerdo Iberico en el periodo predestete con vistas a la caracterizacion productiva preliminar de la raza. Para ello se utilizo una muestra compuesta por 2001 lechones entre machos y hembras donde se estudio el peso al nacimiento y los pesos a los 30 y 60 dias. De los resultados preliminares obtenidos concluimos un peso al nacimiento medio de 1,37 kg, un peso a los 30 dias de vida en torno a 13,49 kg y un peso a los 60 dias o peso de destete en alrededor de 20 kg, destacando la variedad Retinto como la poblacion que presenta mejor capacidad de crecimiento en este periodo fisiologico, quedando a la espera de profundizar este estudio con una muestra animal mas amplia.
Growth factors of the vascular and nervous systems. Functional characterization and biotechnology.

The "ras" superfamily - post-translational modifications and functional regulation, A.I. Magee et al transforming growth factor beta and growth suppression, J. Massague and M. Laiho structure, function and regulation of protein tyrosine phosphatases, N.K. Tonks PIGF - a gene coding for a novel human vascular permeability related protein, D. Maglione et al cell surface activation of latent TGF beta, D.B. Rifkin et al extracellular sequestration and release of fibroblast growth factor - a possible mechanism for indirect angiogenesis, I. Vlodavsky et al cytokine modulation of endothelial cell interaction with tumour cell, D. Lauri and E. Dejana adhesion molecules induced by cytokines on vascular endothelial cells, D.O. Haskard regulation of nerve growth factor in cortical astrocytes and neurons by interleukin-1, phorbol ester and dexamethasone, B.C. Wise et al synthesis and expression of recombinant human NGF in a mammalian cell line, C. Soranzo et al nerve growth factor effects on the neuroimmunoendocrine system - a biobehavioural perspective, E. Alleva and L. Aloe evidence for a role of nerve growth factor in the mammalian visual system, G. Carmignoto et al nerve growth factor pharmacology in peripheral neuropathies - an outlook, G. Vantini et al retinal ganglion neuronotrophic factor and its monoclonal antibody - functional studies, R.M. W. Chau et al functional characterization of the glucocorticoid regulatory unit of the rat alpha-1-acid glycoprotein promoter, D. Di Lorenzo et al insulin-like growth factor 1 and growth hormone levels in patients with type 1 diabetes mellitus, A. Giustina et al biosynthesis of vasculotropin and expression of vasculotropin receptors by cultured cells, H. Moukadiri et al.
In Vitro Metabolism in Preclinical Drug Development

Metabolism is a major determinant governing both pharmacokinetics and clinical response of the majority of drugs and a great deal of effort is now directed at assessing key metabolic parameters in the early stages of drug development. Several in vitro methods are now available for determination of metabolic features, yielding data that predict in vivo behavior of the studied drug molecules. This article focuses on in vitro metabolism in preclinical drug development. Keywords: metabolism; in vitro methods; preclinical drug development; enzymes
The Chromosomes of the Cupressaceae

Summary Following an account of some improved methods for handling the somatic chromosomes of conifers. the karyotypes of representative species of Tetraclinis. Actinostrobus, Callitris, and Fitzroya are illustrated and described. Surprisingly. Fitzroya cupressoides is a polyploid (2n = 4x = 44) and therefore a ranty m the gymnosperms.
Multispecies interactions among a plant parasite, a pollinator and a seed predator affect the reproductive output of an invasive plant, Cytisus scoparius

Interactions between species pairs are almost always mediated by interactions with other species. The outcomes of these multispecies interactions are often difficult to predict and are rarely studied. In addition to their theoretical importance, multispecies interactions are also important for management situations. Where multiple agents are used to control invasive species, interactions between agents may either enhance or reduce the impacts on the target species, or may simply have additive effects. In this study, conducted in a Mediterranean-type woodland in Australia, we examined how the interaction between an invasive legume, Cytisus scoparius (Leguminosae), its pollinator, Apis mellifera and a seed predator, Bruchidius villosus (Bruchidae), are modified by a native hemiparasitic vine, Cassytha pubescens (Loranthaceae). The parasite had a direct negative effect on C. scoparius, reducing flowering by 50% and consequently fruit and seed production. Despite having fewer flowers, infected plants had the same proportion of ‘tripped’ flowers, an indirect measure of pollinator visitation, as uninfected plants; although fruit formed on infected plants it was more likely to abort prematurely. Seed predation by B. villosus was lower on parasite-infected C. scoparius plants than in uninfected plants. Although Ca. pubescens had an antagonistic effect on B. villosus, in consort, the two agents reduced overall seed production by an average of 62%. The acquired parasite Ca. pubescens was more effective in reducing reproductive output than the introduced seed predator, B. villosus, and shows potential as a biocontrol agent for C. scoparius. We documented a subadditive effect of two biological enemies on the invasive species, where the acquired parasite had a stronger effect than the introduced seed predator.
Residual cyst with semisolid content, a clinical case.

Objective: to present a clinical case of a residual cyst in the right maxillary region with semisolid content. Main data of the case: 61-year-old patient who comes to consultation for the realization of upper jaw dentures, during the clinical examination a slight facial asymmetry of the right side is observed; radiographic and tomographic examination revealed a radiolucent lesion in the right premolar region. It is diagnosed as a residual cyst and an enucleation is decided; during the surgical procedure a viscous content of yellowish coloration and semi-solid content is observed. Main comments: it is very rare to find residual cysts with semi-solid content, since they generally have purulent or serosanguinous contents.
Core Decomposition in Multilayer Networks

Multilayer networks are a powerful paradigm to model complex systems, where multiple relations occur between the same entities. Despite the keen interest in a variety of tasks, algorithms, and analyses in this type of network, the problem of extracting dense subgraphs has remained largely unexplored so far. As a first step in this direction, in this work, we study the problem of core decomposition of a multilayer network. Unlike the single-layer counterpart in which cores are all nested into one another and can be computed in linear time, the multilayer context is much more challenging as no total order exists among multilayer cores; rather, they form a lattice whose size is exponential in the number of layers. In this setting, we devise three algorithms, which differ in the way they visit the core lattice and in their pruning techniques. We assess time and space efficiency of the three algorithms on a large variety of real-world multilayer networks. We then move a step forward and study the problem of extracting the inner-most (also known as maximal) cores, i.e., the cores that are not dominated by any other core in terms of their core index in all the layers. inner-most cores are typically orders of magnitude less than all the cores. Motivated by this, we devise an algorithm that effectively exploits the maximality property and extracts inner-most cores directly, without first computing a complete decomposition. This allows for a consistent speed up over a naïve method that simply filters out non-inner-most ones from all the cores. Finally, we showcase the multilayer core-decomposition tool in a variety of scenarios and problems. We start by considering the problem of densest-subgraph extraction in multilayer networks. We introduce a definition of multilayer densest subgraph that tradesoff between high density and number of layers in which the high density holds, and exploit multilayer core decomposition to approximate this problem with quality guarantees. As further applications, we show how to utilize multilayer core decomposition to speed-up the extraction of frequent cross-graph quasi-cliques and to generalize the community-search problem to the multilayer setting.
Intracerebroventricular Anti‐Ang‐(1‐12)‐IgG Improves Baroreflex Sensitivity and Heart Rate Variability in Transgenic (mRen2)27 Hypertensive Rats

Angiotensin (Ang)‐(1‐12) is a novel Ang peptide that may serve as a precursor to Ang II. Intracerebroventricular (ICV) neutralization of endogenous Ang‐(1‐12) lowered systolic blood pressure (SBP) in transgenic (mRen2)27 rats over‐expressing the murine Ren2 gene (‐26 ± 7 mm Hg at day 10; p < 0.05 vs. baseline; n = 6), but not in rats given a renin inhibitor, pre‐immune IgG or artificial cerebrospinal fluid (aCSF) (n = 6 ‐ 10 each). In (mRen2)27 rats, baroreflex sensitivity (BRS) and heart rate variability (HRV) are reduced. To assess whether brain Ang‐(1‐12) neutralization improves BRS and HRV, male (mRen2)27 rats (22‐28 wks old; n = 3 each group) were administered ICV anti‐Ang‐(1‐12)‐IgG (IgG; 2.7 μg/hr), combination (CMB) of anti‐Ang‐(1‐12)‐IgG and a rat renin inhibitor (WFML‐1, 10 μg/kg/day) or aCSF via osmotic pump for 2 wks. On day 11, mean arterial pressure in awake rats was lower in IgG and CMB rats compared with rats given aCSF (116 ± 3, 125 ± 4 and 142 ± 4 mm Hg, respectively, p < 0.05 vs. aCSF). Sequence and spectral analysis of spontaneous BRS and HRV revealed overall better vagal function in IgG treated rats (Seq All: 1.3 ± 0.1 msec/mm Hg; SDRR: 7.5 ± 0.3 msec, respectively) versus the aCSF group (0.6 ± 0.1 msec/mm Hg; 2.5 ± 1.2 msec; p < 0.05). There were no additional improvements in the CMB group over IgG alone. Thus, endogenous brain Ang‐(1‐12), as an active substrate for Ang II production, impairs BRS and HRV. HL‐51952, HL‐56973
Variação da temperatura de superfície em diferentes usos do solo na cidade de São Carlos-SP

O balanco de energia e o fluxo radiativo produzidos nas superficies urbanas sao objetos de poucos estudos no Brasil. O objetivo deste trabalho foi verificar a variacao da temperatura superficial aparente em diferentes bairros na cidade de Sao Carlos-SP. A metodologia fundamenta-se na utilizacao de um termometro digital infravermelho fixado em um tripe, com a afericao de pontos cardeais e colaterais nas angulacoes de 10o, 0o, em diversas classificacoes do uso do solo urbano definidas pelo Laboratorio Quadro do Paisagismo do Brasil - QUAPA. Os resultados apontam que as temperaturas mais elevadas foram registradas para a angulacao -10o, altura aproximada ao nivel do pedestre. Palavras-chave: clima urbano, micro clima, canyon urbano, espacos livres. http://dx.doi. org/10.17127/got/2018.13. 014 Data de submissao: 2017-02-24 Data de aprovacao: 2017-10-29 Data de publicacao: 2018-06-30
Differences in school anxiety, self-esteem and perfectionism according to school level and sex in Chilean primary school students

This study is carried out with the aim of analyzing the school anxiety, self-esteem and perfectionism shown by primary education children, establishing differences regarding gender and school level. The participants were 498 students of 5th and 6th grade belonging to 7 educational centers from Nuble Region, Chile. School anxiety was evaluated with the Visual Analogue Scale for Anxiety-Revised (VAAR). Self-esteem was evaluated with the Self-Esteem Inventory for Primary Education (SEI-P) and perfectionism with the Spanish version of the Child and Adolescent Perfectionism Scale (CAPS). Regarding the school level, there are statistical differences in anxiety levels, the scores are higher in the children of the 5th grade, but in the III domain of self-esteem and considering the total score of perfectionism, students from 6th grade obtain higher scores. Similarly, the results based on gender indicate that the in terms of Critical Self-Oriented Perfectionism and the total perfectionism, boys obtain statistically significant higher scores than girls. These results reflect the complexity of the measured variables and allow to project situated and contextualized psychoeducational interventions.