Miracle-dz/UniFAR-FTUs huggingface dataset:
Impact of IgG3 Subclass and C1q‐Fixing Donor‐Specific HLA Alloantibodies on Rejection and Survival in Liver Transplantation

Recent literature confirms donor‐specific HLA alloantibodies (DSA) impair 5‐year survival in some but not all liver transplant recipients. In an effort to improve DSA testing's association with rejection and death, we retrospectively evaluated 1270 liver transplant recipients for the presence of IgG3 and C1q‐fixing DSA. In patients with preformed DSA, 29 and 51% had IgG3 and C1q‐fixing DSA, respectively. In patients with de novo DSA, 62% and 67% had IgG3 and C1q‐fixing DSA, respectively. When different types of DSA positive patients were compared to DSA negative patients, multivariable analysis showed that IgG3 DSA positivity had the highest numerical hazard ratio for death (IgG3: HR = 2.4, p < 0.001; C1q: HR = 1.9, p < 0.001; standard DSA: HR = 1.6, p < 0.001). Similarly, multivariable analysis demonstrated de novo IgG3 DSA positivity compared to no DSA had the highest hazard ratio for death (IgG3: HR = 2.1, p = 0.004; C1q: HR = 1.9, p = 0.02; standard DSA: HR = 1.8, p = 0.007). Preformed C1q‐fixing class II DSA showed the strongest correlation with early rejection. In conclusion, preformed and de novo IgG3 subclass DSA positive patients had the highest absolute HR for death in side‐by‐side comparison with C1q and standard DSA positive versus DSA negative patients; however, IgG3 negative DSA positive patients still had inferior outcomes compared to DSA negative patients.
Social networks as a tool for job search

The information technology has caused an absolute revolution. Therefore, searching job through social networks is highlighted as it is perspective way how to find a job. At the global level, social networks are used by more than 90% of companies in process of recruitment. Nowadays, social networks are beginning to be applied by Czech and Slovak companies as well. The objective of the research is to identify the differences in the using of social networks in job search in Czech and Slovak Republic. Findings indicate that, only 2% of Czech respondents preferred social networks as a significant way of finding a job. Following the results, social networks were identified less credible by Slovak respondents, but on the other hand more then 4% of Slovak respondents preferred social networks as a toll for job search. Despite the fact that the employees in the Czech Republic and Slovakia do not really use social networks to find employment, this situation can change as respondents express the opinion acoording which social networks have potential for the future. If social networks are used more by employers as well as by potential candidates, both parties will be satisfied.
Analysis of melanoma cells in peripheral blood by reverse transcription‐polymerase chain reaction for tyrosinase and MART‐1 after mononuclear cell collection with cell preparation tubes: a comparison with the whole blood guanidinium isothiocyanate RNA isolation method

&NA; Melanoma cell detection in peripheral blood by tyrosinase reverse transcription‐polymerase chain reaction (RT‐PCR) is usually performed on RNA isolated from whole blood using a guanidinium isothiocyanate (GITC)/phenol extraction method or from Ficoll Hypaque isolated mononuclear cells. The first method contains environmentally harmful reagents, and the second is laborious in the preanalytical steps. Cell preparation tubes (CPTs) are ready‐to‐use Ficoll Hypaque‐based tubes that avoid the time‐consuming and critical loading on Ficoll Hypaque. We examined whether CPTs can be used to determine melanoma cell dissemination in peripheral blood. We first investigated whether melanoma cells were retained in the mononuclear cell layer. All six morphologically different melanoma cell lines studied in the spiking experiments were retained in the upper layer. In further experiments, we were able to detect low dilutions of added SK‐MEL‐28 cells more consistently after nested RT‐PCR for tyrosinase or MART‐1 in the RNA isolated from mononuclear cells from CPTs than from RNA isolated with the GITC method. In addition, RNA was extracted from paired blood samples from 24 analysable stage III and stage IV melanoma patients and analysed for the presence of tyrosinase and MART‐1 RNA using both the CPT/RNeasy and the whole blood/GITC method. The quality of the CPT/RNeasy RNA was better than the RNA isolated from whole blood with GITC/phenol. However, the RT‐PCR results were less unequivocal: MART‐1 mRNA was more often detected with CPT/RNeasy compared with whole blood/GITC (six versus three), whereas tyrosinase mRNA was found less often in CPT/RNeasy RNA (two versus eight). Taken together these results suggest that the CPT isolation method is suitable for the isolation of mononuclear cells, including melanoma cells.
Reactive oxygen species generated by microbial NADPH oxidase NoxA regulate sexual development in Aspergillus nidulans

NADPH oxidases (Nox) have been characterized as higher eukaryotic enzymes used deliberately to produce reactive oxygen species (ROS). The recent discovery of new functional members of the Nox family in plants and animals has led to the recognition of the increasing importance of ROS as signals involved in regulation of diverse cellular processes such as defence, growth and signalling. Here, we address the role of NADPH oxidase‐generated ROS in the biology of the filamentous fungus Aspergillus nidulans. We characterize the noxA gene and show that it encodes a member of a novel NADPH oxidase subfamily ubiquitous in lower eukaryotes. Deletion of noxA specifically blocks differentiation of sexual fruit bodies (cleistothecia), without affecting hyphal growth or asexual development. Accordingly, the noxA gene is induced during sexual development, peaking at the time of cleistothecia differentiation and in parallel with the hülle cell‐associated catalase peroxidase gene cpeA. This expression pattern is not dependent on transcription factors SteA and StuA, which are essential for cleistothecia formation. In contrast, noxA‐dependent premature sexual development correlates with noxA derepression in ΔsakA null mutants, connecting stress MAPK signalling to the regulated production of ROS. Using a nitroblue tetrazolium (NBT) assay to detect superoxide, we found that hülle cells and cleistothecia initials produce superoxide in a process inhibited by NADPH oxidase inhibitor DPI and markedly reduced in ΔnoxA mutants. Furthermore, using H2DCFDA, we detected that H2O2 and possibly other ROS are generated in a NoxA‐dependent fashion, mainly in the external walls from cleistothecia initials. The essential role of NoxA‐generated ROS in A. nidulans sexual differentiation and the presence of one or two noxA homologues in all analysed filamentous fungi suggest that NADPH oxidase‐generated ROS play important roles in fungal physiology and differentiation.
Méthodes numériques pour les écoulements supersoniques avec application au calcul du bruit rayonné par un jet sur-détendu

Cette these est consacree au calcul et a l’etude du champ acoustique rayonne par un jet fortement supersonique, sur-detendu et chauffe. L’approche numerique utilisee consiste a resoudre simultanement l’ecoulement turbulent et le champ proche acoustique par simulation des grandes echelles.Afin de resoudre les equations de Navier-Stokes instationnaires et compressibles, des schemas numeriques de derivation spatiale et d’integration temporelle peu dissipatifs et peu dispersifs sont utilises. Pour le systeme de coordonnees cylindriques, une procedure de deraffinement progressive au voisinage de l’axe du jet est proposee pour augmenter le pas de temps de l’integration temporelle. Une procedure de capture de choc est finalement developpee pour supprimer les oscillations de Gibbs aux voisinages des chocs. Cette procedure est explicite, adaptative et conservative.Un jet rond, sur-detendu et presentant un nombre de Mach d’ejection de 3.30 ainsi qu’une pression et une temperature de reservoir de 28.6 × 105Pa et 1144K est resolu par simulation des grandes echelles. Ce jet est initialement laminaire et possede un nombre de Reynolds de 0.94 × 105. Les champs aerodynamique et acoustique obtenus sont compares aux donnees disponibles dans la litterature. Afin de pouvoir identifier les sources acoustiques,le champ turbulent est caracterise le long de la couche de melange, notamment en utilisant une analyse spectrale et une decomposition selon les modes azimutaux. Les champs acoustiques proche et lointain sont enfin etudies et differentes composantes de bruit sont identifiees a partir de comparaisons avec des mesures et des modeles de la litterature, ainsi qu’en etablissant des liens avec les proprietes de l’ecoulement turbulent. L’influence des effets de la propagation non lineaire des ondes acoustiques est finalement discutee.
Bertini Theorems and Lefschetz Pencils over Discrete Valuation Rings

Good hyperplane sections, whose existence is assured by Bertini’s theorem, and good families of hyperplane sections, so-called Lefschetz pencils, are well-known constructions and powerwful tools in classical geometry, i.e., for varieties over a field. But for arithmetic questions one is naturally led to the consideration of models over Dedekind rings and, for local questions, to schemes over discrete valuation rings. It is the aim of this note to provide extensions of the mentioned constructions to this situation. We point out some new phenomena, and give an application to the class field theory of varieties over local fields with good reduction. See also [JS] for more arithmetic applications. Let A be a discrete valuation ring with fraction field K, maximal ideal m and residue field F = A/m. Let η = Spec(K) and s = Spec(F ) be the generic and closed point of Spec(A), respectively. For any scheme X over A we let Xη = X ×A K and Xs = X ×A F be its generic and special fibre, respectively.
Optically driven CPW fed slot antennas and arrays for wireless communications

This paper presents a comparison of three different types of CPW fed slot antennas integrated with a photodetector. The antennas are driven by optical power converted into microwave power using the photodetector which is directly attached to the antennas without the use of any amplifying devices. The operating frequency of these antennas is around 18.5 GHz. This paper presents the approach for matching a photodetector to a single element or to an array of elements. Measured data and simulations for various antenna/photodetector configurations are compared and discussed.
Dynamic reconfiguration of threads in real-time system working on precision time regime

The paper concerns design of real time systems that meet precision time (PRET) requirements. A new, original architecture of the multithread embedded system with programmable interleaved pipelining is introduced. Main components are described with special attention devoted to the interleave controller. This element of the system is responsible for controlling of the order of threads loaded into the processor's pipeline. The idea of shadow deadline processing arbiter responsible for dynamic reconfiguration of performed threads (tasks) is given. Results of the implementation and simulation of different arbitration schemes are discussed. Conclusions emphasizing the flexibility and advantages of the proposed solution summarize the paper.
A human hepatitis B viral enhancer element.

Fragments of the cloned hepatitis B virus (HBV) genome were assayed in vivo for the presence of a transcriptional enhancer element. We demonstrate that sequences positioned approximately 450 bp upstream from the HBcAg gene promoter are required for its efficient activity. These HBV stimulatory sequences activate transcription when inserted upstream to a heterologous SV40 early promoter. Like other known enhancer elements, this HBV sequence acts in an orientation‐independent manner. Furthermore, the HBV enhancer element exhibits a preferred activity in a human hepatoma cell line.
NEOWISE STUDIES OF SPECTROPHOTOMETRICALLY CLASSIFIED ASTEROIDS: PRELIMINARY RESULTS

The NEOWISE data set offers the opportunity to study the variations in albedo for asteroid classification schemes based on visible and near-infrared observations for a large sample of minor planets. We have determined the albedos for nearly 1900 asteroids classified by the Tholen, Bus, and Bus–DeMeo taxonomic classification schemes. We find that the S-complex spans a broad range of bright albedos, partially overlapping the low albedo C-complex at small sizes. As expected, the X-complex covers a wide range of albedos. The multiwavelength infrared coverage provided by NEOWISE allows determination of the reflectivity at 3.4 and 4.6 μm relative to the visible albedo. The direct computation of the reflectivity at 3.4 and 4.6 μm enables a new means of comparing the various taxonomic classes. Although C, B, D, and T asteroids all have similarly low visible albedos, the D and T types can be distinguished from the C and B types by examining their relative reflectance at 3.4 and 4.6 μm. All of the albedo distributions are strongly affected by selection biases against small, low albedo objects, as all objects selected for taxonomic classification were chosen according to their visible light brightness. Due to these strong selection biases, we are unable to determine whether or not there are correlations between size, albedo, and space weathering. We argue that the current set of classified asteroids makes any such correlations difficult to verify. A sample of taxonomically classified asteroids drawn without significant albedo bias is needed in order to perform such an analysis.
Avian influenza virus A H7N9 infects multiple mononuclear cell types in peripheral blood and induces dysregulated cytokine responses and apoptosis in infected monocytes.

Most patients with avian influenza A H7N9 virus (H7N9) infection suffer from severe illness, accompanied by dysregulated cytokine/chemokine response, delayed viral clearance and impaired neutralizing antibody response. Here, we evaluated the role of peripheral blood mononuclear cells (PBMCs) in the pathogenesis of H7N9 infection using an ex vivo infection model. H7N9 infected a significantly higher percentage of PBMCs (23.9 %) than those of avian influenza A H5N1 virus (H5N1) (12.3 %) and pandemic H1N1 virus (pH1N1) (5.5 %) (P<0.01). H7N9 infected significantly more B and T lymphocytes than H5N1. When compared with pH1N1, H7N9-infected PBMCs had significantly higher mRNA levels of proinflammatory cytokines and type I interferons (IFNs) at 6 h post-infection (p.i.), but significantly lower levels of IFN-γ and IP-10 at 12 h p.i. Among the PBMCs, CD14+ monocytes were most permissive to H7N9 infection. The percentage of infected CD14+ monocytes was significantly higher for H7N9 than that of pH1N1, but not significantly different from that of H5N1. H7N9-infected monocytes showed higher expression of MIP-1α, MIP-1β and RANTES than that of pH1N1 at 6 h p.i. H7N9- but not pH1N1-infected monocytes died rapidly via apoptosis. Furthermore, pH1N1- but not H7N9-infected monocytes showed increased expression of the monocyte activation and differentiation markers. Unlike pH1N1, H7N9 showed similar PBMC/monocyte cytokine/chemokine expression profile, monocyte cell death and expression of activation/differentiation markers to H5N1. Besides proinflammatory cytokine activation leading to a cytokine storm, impaired IFN-γ production, rapid monocytic death and lack of monocyte differentiation may affect the ability of H7N9-infected innate immune cells to recruit protective adaptive immunity.
Scrapie associated fibrils in the diagnosis of scrapie in sheep

Previous research has consistently demonstrated by electron microscopy the presence of scrapie associated fibrils in brain extracts prepared from mice and hamsters with clinical signs of experimental scrapie. In the present study similar fibrils were seen in all the brain extracts prepared from 11 Cheviot or Suffolk sheep with natural or experimental scrapie that had been diagnosed clinically and confirmed neuropathologically. They were not found in the brain extracts of nine sheep that did not have scrapie and which included four that had been injected with infected material but did not develop the disease. The presence of such fibrils can therefore be used as an additional diagnostic criterion for natural scrapie in sheep.
Financial Leverage and Financial Performance: Empirical Evidence From Listed Textile Industry of Pakistan

The textile sector in Pakistan is the largest manufacturing industry and recognizable model of resistance economy. Over centuries, textile sector has been the country’s backbone making available sources of occupation and export returns. Generally, financial leverage is the organizational capability of using borrowed money. It can be described as a fraction, to which a company uses fixed income securities such as debt and equity. The objective of the current study is measuring the impact of financial leverage on firm’s financial condition. It is essential to know whether a positive or negative relationship exists between two terms, financial leverage and financial performance of the textile industry; evidence from the listed firms of Pakistani textile industry from 2007-2016. Pooled regression analysis technique shows that there is positive relationship between financial leverage and financial performance (Returns on Assets, Sales Growth and Net Profit Margin). On the other hand, negative relationship exists between Return on Equity ratio. It is confirmed that the organizations have more profitability, might enhance the financial performance having more levels of financial leverage. This study also gives evidence by estimating different facts. It exposes that the main elements of the textile industry in Pakistan enhance their financial performance by employing the financial leverage strategy and can attain a sustainable future growth by making decisions about the selection of their optimum capital structure.
Acid phosphatase mutants in Chlamydomonas: isolation and characterization by biochemical, electrophoretic and genetic analysis.

In order to isolate acid phosphatase mutants in the green alga Chlamydomonas reinhardi, a staining method for detecting the enzyme activity in colonies has been developed. The occurrence of more than one acid phosphatase brought about some difficulty in the selection of mutants. We have, however, found an original method of selection based on the differential heat sensitivity of the enzymes. After treatment of the wild-type strain with N-methyl-N'-nitro-N-nitrosoguanidine, two types of mutants were recovered, then analyzed by biochemical and electrophoretic methods. In the first class of mutants (P(1), P(2), P(3),. ) a heat-stable acid phosphatase bound to cellular debris of the crude extract was missing. The mutant P(a), representing the second class of mutations, was lacking a soluble heat-sensitive enzyme. These mutations were genetically different and exhibited mendelian inheritance.
The role of light and growth regulators in the opening of the dentaria petiolar hook.

The phenomenon of the etiolated hook is not restricted to the hypocotyl of the dicotyledenous plant (e.g., Phaseolus) but appears to serve a similar, adaptive function in the petioles of certain rhizomatous plants. The commonly employed regulants of hypocotyl hook opening were tested for their effect on the petiolar hook of Dentaria diphylla. The hook was found to require both light (red light promoted, far red inhibited) and the intact leaf for opening. The leaf requirement was fully replaced by gibberellic acid (0.04% in lanolin) but only in light; cobalt chloride (0.1-1.0 mm) promoted a partial opening in dark with or without leaf; and coumarin (1 mm), indoleacetic acid (1-4% in lanolin), and ethylene 10 microliter per liter all inhibited opening of hooks with or without lamina. The absolute requirement for light and leaf tissue and the replacement of proximal tissue by GA(3) alone represent marked differences in the physiology of hypocotyl and petiolar hooks. These differences are believed to indicate the necessity for concomitant leaf maturation in petiolar hook opening.
Multiclass Support Vector Machines with Simultaneous Multi-Factors Optimization for Corporate Credit Ratings

— Corporate credit rating prediction is one of the most important topics, which has been studied by researchers in the last decade. Over the last decade, researchers are pushing the limit to enhance the exactness of the corporate credit rating prediction model by applying several data-driven tools including statistical and artificial intelligence methods. Among them, multiclass support vector machine (MSVM) has been widely applied due to its good predictability. However, heuristics, for example, parameters of a kernel function, appropriate feature and instance subset, has become the main reason for the critics on MSVM, as they have dictate the MSVM architectural variables. This study presents a hybrid MSVM model that is intended to optimize all the parameter such as feature selection, instance selection, and kernel parameter. Our model adopts genetic algorithm (GA) to simultaneously optimize multiple heterogeneous design factors of MSVM.
Chemical images: Technical approaches and issues

Chemical mapping techniques using Raman microscopy are introduced, and using an example of a pharmaceutical tablet, the practical aspects of data collection and processing to produce a chemical image of the sample are detailed. Issues related to data processing, instrument standards, chemical image reportable errors, and the interpretation of chemical images are presented to encourage debate, develop solutions, and promote use in other challenging scientific applications. applications. © 2006 International Society for Analytical Cytology
A dynamic subspace learning method for tumor classification using microarray gene expression data

Among most of the subspace learning methods, Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) are classic ones. PCA tries to maximize the total scatter across all classes. In this case, however, the data set, with a small between-class scatter and a large within-class scatter, can also have a large total scatter. It conflicts with Maximum Margin Criterion (MMC) which tries to maximize the between-class scatter and minimize the within-class scatter. To address the conflict problem, we proposed a dynamic subspace learning method which can balance the objectives of PCA and MMC simultaneously by searching for the best coefficient. Our experiments are implemented by classification on two tumor microarray datasets. Firstly a simple t-test was used for gene selection, then our novel method was applied to gene extraction, and finally we adopted KNN and SVM classifiers to evaluate the effectiveness of our method. Results show that the new feature extractors are effective and stable.
The effects of brand hearsay on brand trust and brand attitudes

ABSTRACT Utilizing Grace and O’Cass’s Service Brand Verdict (SBV) framework, this research suggests that brand hearsay (i.e., advertising/promotion, word-of-mouth, and non-paid publicity) affects brand trust (i.e., affective and cognitive trust), which then leads to forming brand attitudes. This study tests the model and hypotheses based on responses from a sample of 346 respondents recruited by online sampling. A two-stage procedure is conducted to assess the measurement model and structural model using a bootstrapping technique with SmartPLS 3. The findings demonstrate that advertising/promotion and WOM (eWOM) had effects on affective trust while publicity did not have effect on affective trust. Also, advertising/promotion had a direct positive effect on cognitive trust, whereas neither WOM (eWOM) nor publicity did. In the brand trust-attitude link, affective trust not only fosters cognitive trust to brands, but also affects brand attitude positively.