Miracle-dz/UniFAR-FTUs huggingface dataset:
Psychological manifestations in post myocardial infraction patients

Background: In the past few years a very important observation that came to light is the increase in prevalence of some psychiatric disorder in chronically ill patients. Symptoms of depression and anxiety are common following myocardial infarction (MI). Both the symptoms have been linked with increased risk of recurrent coronary artery disease (CAD), Severe psychological stress, major life events, and depression causes myocardial infarction (MI) in 15% of case of Coronary Artery Disease (CAD) and are associated with higher rates of morbidity and mortality. Objective: (1) To establish the diagnosis of myocardial infarction in patients. (2) To study and correlate various psychological manifestations in these patients. Methodology: The patients for the present study were taken up from those admitted in ICCU and OPD of department of Medicine G.M. C, Ambikapur, during the study period. 50 patients were studied having CAD (Myocardial infarction). The patients were history taken in detailed & examined (Physical and Psychiatric), investigated with reference to ECG proven myocardial infarction or cardiac enzymes proven MI. Results: We have found 46% of prevalence rate of depression in our study. Majority of patients (20%) have mild depression (score 10-13) on (HAD-Scale), 12% have major depression, 42% had Anxiety. Majority of patients 26% have mild anxiety. Only 6% have severe anxiety (score > 30) in our study. Conclusion: Personality feature of CAD patients are associated with emotional as sympathetic arousal which result in increases in catecholamine as consequent increase in mobilization of free fatty acid from adipose tissue, aggregation of platelets which may precipitate MI. So, a thorough research, prevention and rehabilitation of psychologically oriented care can increase the patients sense of security and is fundamental basis of successful medical care. ……………………………………………………………………………………………………………………………….
Catalytic Plasticity of the Aspartate/Glutamate Racemase Superfamily

The bacterial and archaeal Asp/Glu racemase enzyme superfamily contains a variety of catalytic functions that have great potential for use in industrial biocatalysis. Members of this superfamily include aspartate racemases (AspRs), glutamate racemases (GluRs), hydantoin racemases (HydRs), arylmalonate decarboxylases (AMDs) and maleate cis-trans isomerases (MIs). Despite their catalytic diversity, all characterised members share the same protein fold, catalytic cysteine residues and reaction intermediate. Attempts to exploit this evolutionary flexibility for new processes have had limited success so far, showing that the employed mechanisms are not yet fully understood. For example, the well-characterised Bordetella bronchiseptica AMD (BbAMD) enantiospecifically decarboxylates a range of arylmalonates was but is not able to decarboxylate alkylmalonates despite considerable efforts made by site directed mutagenesis. In this work an investigation of the sequence diversity of the superfamily was undertaken and a range of BbAMD sequence homologues was tested for both aryl- and alkylmalonate decarboxylation (Chapter 3). However, none of the homologues exhibited decarboxylation activity. Targeted mutation of active site residues in an attempt to introduce decarboxylase activity was also unsuccessful. In an alternative approach to identify new alkylmalonate decarboxylating enzymes, a range of bacterial strains capable of processing alkylmalonates was isolated using selective enrichment from soil samples (Appendix D). The only characterised superfamily enzymes without a described three dimensional protein structure are MIs. In order to illuminate the distinct mechanism of MIs, the activity of the superfamily member Nocardia farcinica MI (NfMI) was characterised (Chapter 4) and its structure was determined by X-ray crystallography (Chapter 5). A potent inhibitor and substrate analogue bromomaleate was found. Mutagenesis of the active site cysteine dyad confirmed its catalytic role and Cys76 was found to be more important than Cys194. The data support a mechanism initiated by nucleophilic attack by Cys76 on the double bond of maleate. Although alternative mechanisms cannot be excluded at present, these findings indicate that the mechanistic chemistry in the superfamily is more adaptable than previously thought.
Prey preferences and dietary overlap amongst Africa's large predators

Abstract Africa supports Earth's richest assemblage of large predators, which coexist despite a high degree of dietary overlap. This study used reviews of the prey preferences of African wild dog Lycaon pictus, cheetah Acinonyx jubatus, leopard Panthera pardus, lion P. leo, and spotted hyaena Crocuta crocuta to investigate the degree of dietary overlap and dietary niche breadth amongst the guild. Wild dogs and cheetahs exhibited the greatest dietary overlap and smallest dietary niche breadth, while lions exhibited the least dietary overlap and, with leopards, had the broadest dietary niche breadth. Increased extinction risk within the guild was related to lower dietary niche breadth. The behavioural and morphological specializations of the two most threatened predators (wild dogs and cheetahs) limit the prey available to them, and increases the potential for dietary competition. Conversely, the large body mass and group hunting strategy of lions and the predatory flexibility of leopards and spotted hyaenas minimizes the effects of dietary overlap, assuring a more secure status. This study intimates reasons why cheetahs and African wild dogs are naturally less common than lions, leopards and spotted hyaenas in unmodified landscapes. The methods used can be applied to all adequately studied faunal guilds and could highlight previously undetected competitors.
Axogial communication mediated by soluble neuregulin-1 and bdnf

AXOGIAL COMMUNICATION MEDIATED BY SOLUBLE NEUREGULIN-1 AND BDNF by ZHENZHONG MA August 2011 Advisor: Jeffrey A. Loeb, M.D., Ph.D. Major: Molecular Biology & Genetics Degree: Doctor of Philosophy During peripheral nervous system development, successful communication between axons and glial cells including Schwann cells in peripheral nervous system and oligodendrocytes in central nervous system, is required for the proper functions of both neurons and glia. Three types of alternatively-spliced proteins belonging to the neuregulin1 (NRG1) gene family of growth and differentiation factors are essential for Schwann cell survival and peripheral nerve development. While membrane-bound NRG1 forms (type III) has been strongly implicated in the regulation of myelination process at late stage of Schwann cell development, little is known about the role of soluble, heparin-binding forms of NRG1 (type I/II) in regulating early Schwann cell development in vivo. These forms are rapidly released from axons in vitro by Schwann cell-secreted neurotrophic factors, and, unlike membrane-bound forms, have a unique ability to diffuse and adhere to heparan sulfate-rich cell surfaces. We harness this natural targeting ability of soluble NRG1 to develop a novel antagonist by fusing its heparin-binding domain (HBD) to the soluble human epidermal growth factor receptor 4
Clinical Evaluation of Post Total Hip / Knee Replacement in Hip / Knee Spine Syndrome

Insiden osteoarthritis pada hip joint , osteoarthritis pada knee joint , dan degenerative lumbar spondylosis semakin meningkat seiring dengan bertambahnya populasi penduduk usia tua. Kasus Hip/Knee Spine syndrome sendiri cukup sering ditemukan. Namun literatur yang mengevaluasi kondisi klinis, khususnya keluhan low back pain pada pasien dengan hip/knee spine syndrome post total hip/knee replacemen t masih sangat terbatas Tujuan dari penelitian ini adalah untuk mengevaluasi efek dari total hip/knee replacement pada pasien dengan hip/knee spine syndrome , berkaitan dengan keluhan low back pain . Penelitian ini adalah observasional retrospektif. Penelitian ini menggunakan 5 orang pasien post total hip/knee replacement yang juga didapatkan keluhan low back pain . Pasien yang terpilih akan dievaluasi klinis dengan menggunakan kuesioner LBP VAS Score , Oswestry Disability Index , Hip Harris Score untuk pasien post total hip replacement , dan Oxford Knee Score untuk pasien post total knee replacement . Dilakukan pengamatan pre dan post operasi pada seluruh pasien. Data pasien didapatkan dari Rumah Sakit Orthopaedi dan Traumatologi Surabaya mulai bulan Juni-November 2015. Berdasarkan test paired samples, didapatkan hasil sig 0,001 (< 0,05) pada skor VAS dan sig 0,033 (<0,05) pada skor ODI. Maka dapat disimpulkan bahwa terdapat perbedaan yang signifikan antara skor VAS LBP dan skor ODI sebelum dan setelah terapi Hip/Knee Replacement pada pasien dengan Hip/Knee Spine Syndrome .
Ichthyoses: Clinical, Biochemical, Pathogenic and Diagnostic Assessment

The various manifestations of ichthyoses are classified either by their appearance or their molecular genetics. This volume focuses on generalized, inherited disorders of cornification, which constitute an ever-enlarging group of monogenic diseases caused by a large number of genes that affect a broad array of cellular functions. The authors' overview reflects their unique perspective that the clinical phenotype in the inherited ichthyoses mirrors a 'best attempt' by a metabolically compromised epidermis to maintain a barrier sufficiently impermeable for survival in a desiccating external environment. The basis for threats to survival is illuminated, and the systemic problems, including growth failure, also reflect a compromised barrier. A new consensus classification of these disorders is provided, and the distinguishing clinical features of each disorder are described. Further, the latest molecular genetic information is succinctly reviewed with up-to-date and comprehensive references. Yet, the major emphases of this volume are on disease pathogenesis and on the identification of key ultrastructural features. This publication will prove an invaluable aid to dermatologists, pediatric dermatologists and pediatricians dealing with patients with inherited ichthyoses. In addition, clinical geneticists and dermatopathologists will find it interesting reading.
Origines et évolution de lignées hydrothermales

De par leur originalite, certains organismes de la faune hydrothermale ont ete classes dans des nouveaux groupes taxonomiques de haut rang. De precedentes etudes moleculaires ont permis d? en reassigner certains dans des lignees connues, occasionnant parfois d? importantes reductions de rangs taxonomiques. Par ailleurs, en phylogenie moleculaire, il est difficile d? optimiser a la fois l? echantillonnage taxonomique et le nombre de marqueurs. Cette these illustre cette limitation, mais fournit des avancees sur la comprehension de l? origine et l? evolution de trois groupes hydrothermaux. Chez les vers Polynoidae, l? approche multigene appliquee sur un grand nombre d? especes suggere au moins deux evenements de colonisation de ce milieu. Toutefois, le manque de resolution des marqueurs pour les n? uds profonds entrave la comprehension de l? histoire de ces colonisations. Une limitation similaire avait empeche de replacer les familles de crevettes Alvinocarididae et de crabes Bythograeidae dans leur infra-ordre respectifs (Caridea et Brachyura). Deux approches de recherche et d? identification de marqueurs sont donc testees pour ces groupes. La premiere, basee sur le sequencage de genome mitochondrial (facilement generalisable), resout les relations profondes des Brachyura et place les especes de Bythograeidae disponibles proches des Xanthidae. La seconde, basee sur le sequencage de transcriptomes permet d? identifier des marqueurs suffisamment conserves pour resoudre les relations inter-familles chez les Caridea. Cette approche est moins generalisable, mais les marqueurs identifies pourront a posteriori etre recherches dans un echantillonnage large par capture a l? aide de sonde.
Long Period Variables in Local Group dwarf Irregular Galaxies

Abstract The long-term SAAO survey of Local Group galaxies in the near-infrared (JHKs) has included five dwarf irregulars (dIrr), namely, NGC 6822, IC 1613, WLM, Sgr dIG and NGC 3109. We have found long-period (Mira) variables in all of them. Most of the Miras, which follow a linear LMC period-luminosity (PL) relation well, are carbon-rich. A small group of oxygen-rich Miras are brighter than the linear PL relation predicts, presumably because they are undergoing hot-bottom burning (HBB).
Novel diarrhoea viruses

Novel Rotaviruses in Animals and Man. Nucleic Acid-Based Analyses of Non-Group A Rotaviruses. Seroepidemiology and Molecular Epidemiology of the Chinese Rotavirus. Enteric Adenoviruses. Astroviruses: Human and Animal. Small Round Viruses: Classification and Role in Food-Borne Infections. The Candidate Caliciviruses. Immunobiology of Norwalk Virus. Toroviridae: A Proposed New Family of Enveloped RNA Viruses. Breda and Breda-Like Viruses: Diagnosis, Pathology and Epidemiology. Comparative Pathology of Infection by Novel Diarrhoea Viruses. Clinical Trials of Rotavirus Vaccines. The Diagnostic Gap in Diarrhoeal Aetiology. Final General Discussion. Chairman's Closing Remarks. Index of Contributors. Subject Index.
Molecular analysis of the rlsA gene regulating levan production by the fireblight pathogen Erwinia amylovora

Abstract Natural Erwinia amylovora can display a reduced level of levan synthesis. A gene ( rlsA ) responsible for the suppression of low levansucrase synthesis by E. amylovora was cloned by complementation of natural levan-deficient strains lacking levansucrase expression. The rlsA region was sequenced, and an open reading frame of 266 amino acids was found. Sequence analysis revealed that RlsA resembles a transcriptional activator and may be a member of the LysR family. Overexpression of rlsA from a high copy number plasmid led to a two-fold increase of levan production, but had no influence on amylovoran synthesis. A mutant created by site-directed mutagenesis of wild type strain Eal/79 had the same phenotype as natural levan-deficient strains. The rlsA gene of natural levan deficient strains (with the exception of strain PD494) could be amplified by PCR, yielding the same DNA fragments as wild type strains. The gene was absent in a strain with a large deletion affecting the hrp region of E. amylovora . A match of the nucleotide sequence of rlsA with a sequence database revealed its identity with an incomplete open reading frame located downstream of two recently characterised dsp genes.
Caracterização morfológica e climática da sub-bacia do córrego Cana-Brava do município de Guaraciama – MG

O objetivo deste trabalho foi caracterizar morfometrica e climaticamente a sub-bacia hidrografica do corrego Cana-Brava do municipio de Guaraciama - MG, alem de gerar modelos topograficos da area. Esta sub-bacia e representativa da regiao do Alto Jequitinhonha, sendo pertencente a UPGRH – JQ1. As informacoes foram extraidas da carta topografica vetorial do mapeamento sistematico do municipio de Bocaiuva – MG, com o intuito gerar os resultados dos indices morfometricos e os modelos topograficos da area de estudo. Os softwares utilizados para estas atividades foram o AutoCAD 2004 e o ArcGIS 9.1. Tambem foram extraidas informacoes de series historicas normais mensais e anuais de temperatura media, precipitacao media, umidade relativa e evaporacao total, disponiveis nas Normais Climatologicas (1961 a 1990) de 8 estacoes climatologicas do estado de Minas Gerais proximas a area de estudo, com o intuito de espacializar o parâmetro pelo software ArcGIS 9.1. Contudo, em se tratando da caracterizacao morfometrica, observou-se que a sub-bacia possui tendencia mediana a grandes enchentes, media densidade de drenagem e predominância de relevo plano na area. Em termos de clima, pode-se concluir que ha sazonalidade da precipitacao e inexistencia de periodo de inverno caracteristico . E por fim, o software ArcGIS 9.1 apresentou-se com um importante e fundamental instrumento para estudos relacionados a hidrologia.
Molecular and biochemical characterisation of SiaP as a sialic acid binding protein component of a TRAP transporter of sialic acid

Sialic acid utilisation plays an important role in the growth and persistence of the obligate human mucosal pathogen Haemophilus influenzae, which causes respiratory tract infections, septicaemia and meningitis. Like many other bacteria, H. influenzae can use host-derived sialic acids as carbon, nitrogen and energy sources, but also as a terminal modification on the LPS to better evade the human immune system. H. influenzae takes up exogenous sialic acid via a tripartite ATP-independent periplasmic (TRAP) transporter, SiaPQM. This possesses an extracytoplasmic substrate binding protein (SBP), SiaP, which binds the substrate in the periplasm and delivers it to the specific membrane permease, SiaQM. SiaP contains two globular domains, which close around the substrate upon binding. Here, the mechanism of sialic acid binding by SiaP is investigated using site-directed mutagenesis of residues in the ligand binding site and analogues of sialic acid. These, and several mutations on the surface of SiaP, were investigated for their effect on transport by SiaPQM in vitro, using SiaQM reconstituted into proteoliposomes, and in vivo, using expression of siaPQM in an E. coli strain lacking its native sialic acid transporter, NanT. It is demonstrated that stabilisation of the carboxylate group of sialic acid by the totally conserved Arginine-147 is important for high-affinity ligand binding, but is not essential for transport. Mutation of Aparagine-150 to Aspartate abolishes the function of the transporter without affecting ligand binding, suggesting the existence of a critical interaction between the components of the transporter. The catabolism of the sialic acid analogues was also examined in E. coli expressing different sialic acid transporters. This indicates that a wide variety of sialic acid analogues are potential carbon sources in many pathogenic bacteria.
Seasonal flight dynamics of the apple clearwing moth (Synanthedon myopaeformis Borkh., Lepidoptera: Sesiidae) based on catches in pheromone traps

Recently, the apple clearwing moth, Synanthedon myopaeformis Borkh. (Lepidoptera: Sesiidae), proved to be an economically important pest in Bulgaria. Possibilities for monitoring of the pest by pheromone traps were studied in the years 2003-2005. The investigations were carried out in experimental apple orchards of the Fruit Growing Institute and Agricultural University in Plovdiv. The effectiveness of Delta sticky traps and dry funnel traps were tested. Dry traps and pheromone dispensers were products of Csalomon® (Institute of Plant Protection, Hungarian Academy of Sciences, Budapest) while Delta sticky traps were home-made in Bulgaria. The pheromone traps showed a satisfactory effectiveness and selectivity. The flight of apple clearwing moth began in the middle of May and lasted from three to three and half months. Mass flight occurred between the middle of June and the end of July. Sticky and funnel traps may be successfully used for monitoring flight activity of apple clearwing moth.
Proportionate spatial sampling and equal‐time sampling of mobile animals: A dilemma for inferring areal dependence

Patch or island area is one of the most frequently used variables for inference in conservation biology and biogeography, and is often used in ecological applications. Given that all of these disciplines deal with large spatial scales, exhaustive censusing is not often possible, especially when there are large numbers of patches (e.g. for replication and control purposes). Therefore, data for patches or islands are usually collected by sampling. We argue that if area is to be used as an inferential factor, then the objects under study (i.e. the patches) must be characterized on an areal basis. This necessarily means that fixed-area sampling is inadequate (e.g. a single standard quadrat or transect set within patches irrespective of the patch area) and that some form of area-proportionate sampling is needed (e.g. a fixed areal proportion of each patch is surveyed by random allocation of standard quadrats across each patch). However, use of area-proportionate sampling is not usually dissociated from the increased temporal intensity of sampling that arises from using this approach. The dilemma we see is deciding how much of the area-specificity of variables such as species richness, rare-species indices or probabilities of occurrence of individual species is related to the area-proportionate survey protocol and how much is due to the temporal intensity of surveys. We undertook a study in which we balanced temporal and spatial effects by increasing the time spent surveying smaller patches of vegetation to account for the area-ratio difference. The estimated species richness of birds of the box-ironbark system of central Victoria, Australia, was found to depend strongly upon area when area-proportionate sampling alone was performed. When time-balancing was imposed upon area-proportionate sampling, the differences between smaller (10-ha) and larger (40-ha) areas were much reduced or effectively disappeared. We show that species found in the additional surveys used to conduct the time-balancing were significantly less abundant than species recorded in area-proportionate sampling. This effect is probably most severe for mobile animals, but may emerge in other forms of sampling.
Influence of coastal eddies and counter-currents on the influx of spiny lobster, Panulirus argus, postlarvae into Florida Bay

Postlarvae of the spiny lobster Panulirus argus migrate from offshore in the Florida Keys into their juvenile habitat in Florida Bay through interisland channels. The influx of postlarvae was monitored monthly over the new-moon period at Long Key and Whale Harbor channels (July 1997-June 1999). Although the channels were only 30 km apart, their influx patterns differed. At Long Key, influx peaked every 2-3 months, whereas at Whale Harbor the peaks were in winter and of higher magnitudes. The influx pattern at Long Key was highly correlated with the strength of coastal counter-current flow in the two-week period prior to sampling. Counter- current flow was correlated with alongshore (upstream) wind stress, but the latter was not a significant predictor of postlarval influx. Coastal counter-current flow is hypothesized to indicate the presence of a cyclonic, mesoscale eddy offshore. Satellite imagery confirmed the presence of these eddies offshore of the Middle Florida Keys often when positive postlarval influx and counter-current anomalies were observed. These eddies can facilitate onshore larval transport, and their variable temporal and spatial properties can cause transport variability over a scale of several tens of kilometres along the Keys.