Miracle-dz/UniFAR-FTUs huggingface dataset:
Spatial distribution of active genes implicated in the regulation of insulin‐like growth factor stimulatory loops in human decidual and placental tissue of first‐trimester pregnancy

We have previously shown that the insulin‐like growth factor‐2 (IGF‐2) gene is partially coexpressed with the IGF‐1 and ‐2 receptor genes in proliferative cytotrophoblasts of the human extraembryonic tissue. Here we show that high levels of IGF‐2 gene expression are not restricted to the embryonic tissue but can also be found in the decidua compacta. The IGF‐2 gene is thus expressed at high levels in the mesenchymal stroma of the decidua to establish potentially short‐range communication with primarily IGF‐1 receptor‐positive mesenchymal stroma cells. Conversely, the glandular and surface epithelia coexpress the IGF‐1 receptor and IGF‐1 genes, while the IGF‐2 gene is not detected above background levels. The potential control machanisms of these cell‐cell signalling pathways were investigated by the analysis of the spatial distribution of active IGF binding proteins (IGFBP) genes. The IGFBP‐3 gene is coexpressed with the IGF‐2 gene in proliferative cytotrophoblasts of the embryonic placenta. While active IGFBP‐1 and ‐2 genes in our hands cannot be detected in the embryonic placenta, all three IGFBP genes are expressed in complex and overlapping patterns in the decidua compacta. The results are discussed in terms of how the various IGFBP genes may operate in different cell types to restrict IGF local stimulatory pathways. © 1992 Wiley‐Liss, Inc.
Venous Thromboembolism in Pediatric Patients: Epidemiologic Data From a Pediatric Tertiary Care Center in Alabama

Venous thrombosis is an infrequent but serious cause of hospitalization in children. The epidemiology and natural history remains incompletely defined, especially in geographically distinct regions of the United States. We thus evaluated thrombosis in a single children's hospital over a 3-year period. Of 41,906 hospitalizations, 92 children were identified for review. The incidence of thrombosis was 21.9 per 10,000 admissions (0.22%). Venous thrombosis was of equal incidence in African-American and white patients. Locations of thrombosis included deep venous (51%), pulmonary (21%), renal vein (8%), intrahepatic (8%), and intracranial (12%). Risk factors for thrombosis included central catheter (32%), malignancy (18%), systemic infection (21%), neurologic disability (9%), cardiac (4%), nephrotic syndrome (3%), and autoimmune (6%). Six of 92 patients (7%) had thrombophilia. Positive family history of venous thromboembolism (VTE) or thrombophilic disorder predicted an abnormal test. Treatment included low-molecular-weight heparin (n=53), coumadin (n=12), heparin (n=10), tissue plasminogen activator (n=6), argatroban (n=1), thrombectomy (n=2), inferior vena cava filter (n=2), and no treatment (n=23). Seventy-seven percent demonstrated resolution of the VTE, 14% had persistent or recurrent VTE, and 9% died. Causes of death were malignancy, prematurity, septicemia, and congenital heart disease. Venous thrombosis is a serious comorbidity in hospitalized children. In our population, African-Americans had an equal incidence of VTE as whites. Positive family history predicted thrombophilia.
Hospitalisation of children aged 0–59 months with rotavirus gastro-enteritis before the introduction of routine vaccination (Sicily 2003–2012)

Background: Recent evidence demonstrates that rotavirus vaccination is the best strategy for reducing rotavirus gastro-enteritis (RVGE) in young children. Aims: This study describes the epidemiology of RVGE hospitalisation of Sicilian children before universal rotavirus vaccination was introduced into the regional immunisation programme in January 2013. Methods: An observational study was undertaken by analyzing data obtained from the Regional Hospital Discharge database, including hospitalisation from 2003 to 2012 of subjects aged 0–59 months who lived in Sicily. Children discharged with the rotavirus-specific ICD-9-CM code of 008·61 on first or any diagnosis stage were considered to be RVGE cases. Results: From 2003 to 2012, 9317 children (median age 19 months, M/F ratio 1·19) were hospitalised with a diagnosis of RVGE. During the study period, annual rates of hospitalisation were between 2·64 and 4·68 cases/1000 children (mean 3·74 cases). Incidence rates were higher in children aged 6–11 months (8·85/1,000 children/year), decreasing significantly with age (P<0·001). RVGE hospitalisation peaked during winter and spring with a statistically significant downward trend throughout summer and autumn. Conclusion: During the study period, the cumulative risk of hospitalisation with RVGE was determined to be about one in 54 Sicilian children in the 1st 5 years of life. Although the present study cannot be considered a proper pharmaco-economic evaluation, the findings suggest that in Sicily the health and economic burden of RVGE hospitalisation strongly supports the introduction of rotavirus vaccination into the regional immunisation programme as a probably cost-saving intervention.
A Virtual Machine Cloning Approach Based on Trusted Computing

Cloud computing has become more and more popular and the technology of rapid virtual machine (VM) cloning is widely used in cloud computing environment to implement the fast deployment of VMs to meet the need of bursting computational request. However, the security issue of VM cloning technology is not considered thoroughly in current approaches. This paper proposes a virtual machine cloning approach based on trusted computing which deals with memory and disk of a VM separately. This approach resolves three problems: the identities verification of involved servers; the attestation of source VM and destination VM; the protection of integrity of transmitted data. DOI: http://dx.doi. org/10.11591/telkomnika.v11i11. 3558 Full Text: PDF
Law and disorders: illness/disability and the experience of everyday problems involving the law

The Legal Australia-Wide (LAW) Survey has demonstrated a strong relationship between the prevalence of legal problems and long-term illness/disability, with elevated levels for all types of illness/disability and particularly for mental illness. This paper draws on the LAW Survey data to explore how people with different types of illness/disability respond to the legal problems they face. The findings indicate that such people take action to resolve legal problems and seek formal legal and non-legal help at similar or higher rates than others. Given that people with a disability can face obstacles in accessing legal services, the findings may reflect greater need for help in dealing with legal problems or greater access to support services, which may afford more opportunity for referral.
Plasminogen Activation by Receptor-Bound Urokinase

The specific cellular receptor for urokinase-type plasminogen activator (uPA) is found on a variety of cell types and has been postulated to play a central role in the mediation of pericellular proteolytic activity. We have studied the kinetics of plasminogen (Plg) activation catalyzed by uPA specifically bound to its receptor on the human monocytoid cell-line U937 and demonstrate this process to have properties differing widely from those observed for uPA in solution. The solution-phase reaction was characterized by a K,,, of 25 p~ and for the cell-associated reaction this fell 40-fold to 0.67 p ~ , below the physiological Plg concentration of 2 p ~ . A concomitant 6-fold reduction in kc,, resulted in an increase in the overall catalytic efficiency, kCat/K,,,, of 5.7-fold. This high affinity Plg activation was abolished in the presence of a Plg-binding antagonist. In contrast to intact cells, purified uPA receptor (isolated from phorbol 12-myristate 13-ace-tate-stimulated U937 cells) was observed to partially inhibit uPA-catalyzed Plg activation, although activity against low molecular weight substrates was retained. Therefore, the cellular binding of Plg appears to be of critical importance for the efficient activation of Plg by receptor-bound uPA. Plasmin generated in the cell-surface Plg activation system described here was also observed to be protected from its principal physiological
Evolution of RNA viruses.

The evolution of RNA viruses has received a great deal of attention in the past several years. The subject is based on information very different from that considered: in other evolutionary discussions. As obligate intracellular parasites, viruses leave no fossil record; indeed the oldest historical accounts describing symptoms believed to be caused by viruses are only a millenium or two old (51, 73). Recently, however, the complete nucleotide sequences of the genomic RNAs of a number of viruses belonging to different virus families have been obtained, and these make possible detailed comparisons of the families on a molecular level. These comparisons have led to new insights into RNA virus evolution, including the beginning of taxonomy based on the relatedness of their genomes, a better understanding of the radiation of viruses to different hosts, and renewed speculation on the origin of viruses. In addition, partial or complete sequences have been obtained for many different strains of certain viruses or for different members of a given family. The comparative sequence data have led to a greater understanding of the rate of divergence of RNA viruses in nature and in cell culture. All of these studies, however, have been of viruses currently extant; the oldest virus isolates date back only to the turn of this century.
Web Image Search Using Attribute Assisted Re- Ranking Model

The massive growth of digital images over the web, required the best image retrieval techniques that can improve the retrieval accuracy of the images. Hence research focus has been shifted from designing of sophisticated algorithms that can reduce the semantic gap between visual features and the richness of human semantics. Hence many image re-ranking technique has been proposed to enhance the text based image results by taking the advantage of visual information contained in the images. But this e arlier techniques are based on the low level visual features. Hence the semantic attributes and low level features are exploited simultaneously by using hypergraph re-ranking method. A hypergraph model the relationship between the images by as per its rele vance score to order the images. Its simple belief is that visually analogous images should have related ranking scores. This modelling connection among more close samples will be able to domain the robust semantic similarity and thus expedite the great ranking performance. Keyword: - Search, Hypergraph, Attribute-assisted, image re-ranking.
The study of prevalence and antimicrobial susceptibility of tracheal bacterial strains isolated from pediatric patients.

The aim of this study was to investigate the prevalence and antimicrobial susceptibility of bacterial strains isolated from tracheal specimens obtained from pediatric patients admitted to a major children hospital in Tehran, in 2007. Tracheal specimens were cultured on the appropriate bacteriological media. Bacterial isolates were identified by standard biochemical and serological tests. Antimicrobial susceptibility testing was performed according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Pseudomonas spp. was identified as the most prevalent bacterial isolate (32%) followed by Staphylococcus aureus (27.6%). Thirty strains (16%) were identified as Klebsiella spp. , 18 (9.6%) as Enterobacter spp. and the rest belonged to coagolase negative Staphylococci, Streptococcus viridans, Acinetobacter spp. , Escherichia coli and Neisseria spp. All Pseudomonas spp. were resistant to ampicllin, kanamycin and ceftizoxime. Staphylococcus and Klebsiella spp. showed high degree of resistance to 40% of examined antibiotics.
Bench to bedside advances in the 21st century for primary headache disorders: migraine treatments for migraine patients.

Primary headache disorders represent a remarkable opportunity for clinical neuroscience to improve patients’ lives in substantial numbers and reduce societal economic burden (Olesen et al. , 2012). Of the neurological maladies (Goadsby et al. , 2002; Akerman et al. , 2011) one could never be accused of bloviating when presenting the impact or opining on the need for investment in research in headache disorders, particularly migraine (Shapiro and Goadsby, 2007). Migraine alone is the sixth leading cause of disability of any condition, and was the leading cause of neurological disability, globally in 1990; it is exactly sixth again in 2013 (Global Burden of Disease Study, 2015). It seems a scandal that major funders have not addressed the issue. As an example, the US National Institutes of Health – NIH-Wide Strategic Plan (https://www.nih.gov/about-nih/nih-wide-strategic-plan) identifies headache disorders and migraine as the most underfunded of all conditions when the burden of the disorder is considered; yet we await any serious attempt to resolve this conundrum. However, as we move into the 21st century there is much to be excited about as we strive to treat migraine patients with migraine medicines, as opposed, particularly in preventive management, to the rag-tag collection of hand-me-downs currently available (Goadsby and Sprenger, 2010). Of the strategies outlined in Table 1, those targeted at calcitonin gene-related peptide (CGRP)-based mechanisms are clearly front-running in terms of both excitement and likely impact. The CGRP story is set out elsewhere in detail (Ho et al. , 2010). In brief, studies in the 1980s (Goadsby et al. , 1983; McCulloch et al. , 1986) led the authors to be interested in the question of whether neuropeptides, such as CGRP or substance P, could be used to track trigeminovascular activation in experimental animals in a manner that could be translated …
Methicillin-resistant staphylococci

Strains of staphylococci resistant to methicillin were identified immediately after introduction of this drug. Methicillin-resistant strains have unusual properties, the most notable of which is extreme variability in expression of the resistance trait. The conditions associated with this heterogeneous expression of resistance are described. Methicillin resistance is associated with production of a unique penicillin-binding protein (PBP), 2a, which is bound and inactivated only at high concentrations of beta-lactam antibiotics. PBP2a appears to be encoded by the mec determinant, which also is unique to methicillin-resistant strains. The relationships between PBP2a and expression of resistance and implications for the mechanism of resistance are discussed. The heterogeneous expression of methicillin resistance by staphylococci poses problems in the detection of resistant strains. Experience with several susceptibility test methods is reviewed and guidelines for performance of these tests are given. Treatment of infections caused by methicillin-resistant staphylococci is discussed. Vancomycin is the treatment of choice. Alternatives have been few because methicillin-resistant strains often are resistant to multiple antibiotics in addition to beta-lactam antibiotics. New agents which are active against methicillin-resistant staphylococci are becoming available, and their potential role in treatment is discussed.
Peripheral neuropathies associated with monoclonal proteins: A clinical review

Over the last decade, the increasing use of serum and urine protein electrophoretic screening of patients with idiopathic peripheral neuropathy has led to greater recognition of peripheral neuropathy syndromes that are associated with monoclonal proteins and plasma cell dycrasias. After careful evaluation, most of these patients have benign monoclonal gammopathy, followed in frequency by primary systemic amyloidosis and osteosclerotic myeloma, with occasional cases associated with osteolytic multiple myeloma, Waldenstrom's macroglobulinemia, gamma heavy chain disease, and other rare disorders. Several of these syndromes have distinctive presentations and are recognizable clinically, whereas others (especially multiple myeloma neuropathy) are diverse clinically and are not clearly distinguishable from other chronic neuropathies. The discovery of lgM‐kappa monoclonal proteins directed at myelin antigens in some patients with benign monoclonal gammopathy and the delineation of the syndrome of neuropathy and multiorgan involvement in osteosclerotic myeloma are important developments which may shed light on the mechanism of the remote effects of malignancies on the nervous system.
Pertuzumab Protects the Achilles' Heel of Trastuzumab—Emtansine

Trastuzumab emtansine (T-DM1) represents a significant advancement in the treatment of HER2+ breast cancers. Its clinical efficacy however will be limited by the development of therapeutic resistance. In this report, the HER3 ligand neuregulin is shown to mediate T-DM1 resistance, which was overcome by administration of pertuzumab, a steric inhibitor of HER2 dimerization. Clin Cancer Res; 20(2); 278–80. ©2013 AACR.
A Functional Driver Analyzing Concept

It is evident that a lot of accidents occur because of drowsiness or inattentiveness of the driver. The logical consequence is that we have to find methods to better analyze the driver. A lot of research has been spent on camera-based systems which focus on the driver's eye gaze or his head movement. But there are few systems that provide camera-free driver analyzing. This is the main goal of the work presented here which is structured in three phases, with the operational goal of having a working driver analyzer implemented in a car. The main question is: is it possible to make statements concerning the driver and his state by using vehicle data from the CAN Bus only? This paper describes the current state of driver analyzing, our overall system architecture, as well as future work. At the moment, we focus on detecting the driving style of a person.
Predominance of a Rare Type of HIV-1 in Estonia

An earlier study has indicated that a complex recombinant HIV-1 strain dominates the epidemic in Estonia. The objective of this study was to further investigate the molecular epidemiology and genetic structure of HIV-1 in Estonia. Most of the investigated individuals became infected after August 2000 when HIV-1 started to spread rapidly among Estonian intravenous drug users (IDUs). Two viral DNA regions, gag/pol and gp41, were sequenced and subtyped from peripheral blood mononuclear cells or plasma from 141 individuals. Phylogenetic analysis in the gp41 region revealed that the most frequent type of the virus among IDUs was a circulating recombinant form, CRF06_cpx, whereas a few samples showed highest sequence similarity to a subtype A strain circulating in Ukraine and Russia. Likewise, in the gag/pol region, most of the samples were classified as CRF06_cpx, with a few classified as subtype A. In this region, however, 16% of the sequences turned out to be mosaic unique recombinant forms consisting of CRF06_cpx and subtype A. At least 9 mosaic forms were identified, each with distinct patterns of multiple crossover. To characterize Estonian CRF06_cpx as well as recombinant isolates in more detail, 4 near-full-length HIV-1 genomes were sequenced.
Higher opioid doses predict poorer functional outcome in patients with chronic disabling occupational musculoskeletal disorders.

BACKGROUND Opioids are frequently used for the postoperative treatment of chronic disabling occupational musculoskeletal disorders. In many such cases, long-term opioid use persists because of patient requests for ongoing pain relief. Little is known about the relationship between chronic opioid use and functional recovery in these patients. METHODS A total of 1226 patients with a chronic disabling occupational musculoskeletal disorder were consecutively admitted into an interdisciplinary functional restoration program. They were divided into two groups: 630 patients who reported no opioid use at the time of admission (No group) and 596 patients who reported some opioid use at the time of admission (Yes group). The 516 patients for whom daily opioid doses could be determined were further divided into four subgroups: Low (<30 mg, n=267), Medium (31 to 60 mg, n=112), High (61 to 120 mg, n=78), and Very High (>120 mg, n=59). During the initial weeks of treatment, patients consented to be weaned from all opioid medications. In addition, the patients were assessed before and after rehabilitation with regard to self-reported measures of pain, function, and depression and were analyzed for change. One year after the termination of treatment, socioeconomic outcomes were assessed to measure work and financial status, healthcare utilization, and recurrent injury-associated pain. RESULTS A higher post-injury opioid dose was associated with a greater risk of program noncompletion, which was anticipated because of the requirement that patients taper opioids. High opioid use was significantly related to important socioeconomic outcomes, such as lower rates of return to work and work retention as well as higher healthcare utilization (p<or=0.05 for all). Moreover, at one year after treatment, the group reporting the highest opioid use was 11.6 times as likely to be receiving Social Security Disability Income/Supplemental Security Income as compared with the group reporting no opioid use at the time of admission into the program. CONCLUSIONS Chronic opioid use beginning after a work-related injury is a predictor of less successful outcomes for patients whose final treatment intervention is an interdisciplinary functional restoration program. Higher dose levels are associated with progressively greater indemnity and medical costs for ongoing disability. Physicians involved in the treatment of chronic disabling occupational musculoskeletal disorders should be aware of problems associated with permitting long-term opioid use in patients with a disabling occupational disorder.
Tromboangeítis obliterante: reto a la medicina y a la rehabilitación. Presentación de un caso

Thromboangiitis obliterans (TAO) is few frequent in our environment, with occlusive arterial that occurs mainly in the small and medium-sized vessels, of unknown cause with humoral and immunological factors that affect peripheral microcirculation. One case is presented who finished with bilateral amputation of the lower limbs, with heavy smoking and physical, mental and family repercutions, without getting work installation. Bibliography was reviewed, it’s underline the importance of smoking in the disease onset and its maintenance, its epidemiology, its diagnosis criteria, complementary studies, proposed treatments and its natural evolution. The final outcome of present case was failed. Iloprost is a available drug only in Europe. Transference surgery of omentus, sympathectomy and spinal cord stimulation had not been effectives. Amputation of lower limbs is a final resource for saving patient’s life.
Progress on the GAMS-6 double crystal γ-spectrometer

Measuring the wavelength of gamma-rays emitted after a neutron capture reaction allows a direct determination of the molar Planck constant NAh. Currently these measurements are realized using the high resolution double crystal spectrometers GAMS4 and GAMS5 placed at the high flux reactor of the Institut Laue-Langevin in Grenoble, France. So far it was possible to achieve a relative uncertainty of 4 times 10-7. In this contribution we describe the progress in building a new generation crystal spectrometer, GAMS 6, which is meant to lower the uncertainty to the 10-8 level.