Miracle-dz/UniFAR-FTUs huggingface dataset:
Application of modified atmosphere packaging on aquacultured fish and fish products: A review

Abstract The aquaculture industry has undergone a rapid and continuous growth during the last decade due to increased demands in seafood consumption and reduced pelagic fish production. The aquaculture sector can provide products with a consistent flow and quality standards to cover the market's needs. The modified atmosphere packaging (MAP) technique has evolved over the last two decades and many reports indicate its beneficial effect on many quality parameters during fish and shellfish preservation. The use of MAP can clearly offer an advantage to the safer distribution of quality aquacultured fishery products. This article summarizes most of the experimental data of packaging techniques applied (MAP, VP, various pretreatments and packaging materials) on aquacultured fish and fish products to provide a clear view of the potential for a future commercial use.
Unique regulation of c-Jun N-terminal kinase by PYK2/CAK-beta in angiotensin II-stimulated vascular smooth muscle cells.

Activation of tyrosine kinases is believed to play a central role in angiotensin II (AngII) signaling. Here, we have investigated whether a tyrosine kinase, PYK2, is functionally involved in AngII-induced c-Jun N-terminal kinase (JNK) activation in vascular smooth muscle cells (VSMCs). Adenovirus expressing PYK2 kinase-inactive mutant K457A or a tyrosine phosphorylation site mutant Y402F was transfected in VSMCs. AngII-induced JNK phosphorylation was markedly enhanced by K457A, whereas it was suppressed by Y402F. Protein synthesis induced by AngII was also enhanced by K457A and inhibited by Y402F. In this regard, K457A suppressed PYK2 kinase activation by AngII, whereas it enhanced AngII-induced PYK2 Tyr(402) phosphorylation. By contrast, Y402F inhibited PYK2 Tyr(402) phosphorylation, whereas it markedly enhanced AngII-induced PYK2 kinase activation. Thus, we conclude that PYK2 kinase activity negatively regulates JNK activation and protein synthesis, whereas Tyr(402) phosphorylation positively regulates these events in AngII-stimulated VSMCs, suggesting a unique role of PYK2 in mediating vascular remodeling.
Maternal Plasma Prolactin Levels in Preeclampsia

Maternal plasma prolactin, estradiol-17β, and free estriol levels were estimated in 118 normal pregnancies and in 90 patients with preeclampsia at 35 to 40 weeks' gestation. In the preeclamptic patients, the prolactin values were similar to those of normal pregnancy; the levels were not affected by the severity of disease. At 37 to 38 weeks' gestation the maternal plasma free estriol levels correlated positively with the prolactin levels in preeclampsia (r = .313; P < .05; N = 64). No correlation was found between the levels of prolactin and estradiol-17β or between prolactin and urinary estrogen. Fetal distress was related to decreased plasma estradiol levels, but this was not reflected in the prolactin concentrations. The results of the present study show that the estimation of plasma prolactin level is of no clinical significance in patients with preeclampsia.
To Transmit or Not to Transmit: A Discrete Event-Triggered Communication Scheme for Networked Takagi–Sugeno Fuzzy Systems

This paper first proposes a discrete event-triggered communication scheme for a class of networked Takagi-Sugeno (T-S) fuzzy systems. This scheme has two main features: 1) Whether or not the sampled state should be transmitted is determined by the current-sampled state and the error between the current-sampled state and the latest transmitted state. Compared with those in a periodic time-triggered communication scheme, the communication bandwidth utilization is considerably reduced while preserving the desired control performance; and 2) it is a discrete event-triggered communication scheme due to the fact that the triggered conditions are only measured and checked at a constant sampling period. Compared with a continuous event-triggered communication scheme, the special hardware for continuous measurement and computation is no longer needed. Second, a networked T-S fuzzy model is delicately constructed, which not only considers nonuniform time scales in the networked T-S fuzzy model and the parallel distributed compensation fuzzy control rules but includes the aforementioned state error as well. Third, a stability criterion and a stabilization criterion about the networked T-S fuzzy system are derived, respectively. The stability criterion and stabilization criterion can provide a tradeoff to balance the required communication resource and the desired performance: Lowering the desired performance allows the network to allocate more limited bandwidth to other nodes in need. Finally, a numerical example is given to show the effectiveness of the proposed method.
Asian medical marketing, a review of factors affecting Asian medical tourism development

ABSTRACT Recently, medical tourism has been considered as a profitable economic sector in developing countries. In this study, we have reviewed articles from 2000 to 2017 on medical tourism marketing in Asian countries. We have found that perceived service quality and satisfaction are the most important factors to attract medical tourists. Moreover, a lack of factors like coordination among medical market stakeholders, medical services quality, insurance coverage, and effective laws are the major barriers to medical travel cited in studies. The results suggest that more specific models should be presented for Asian medical marketing, especially in niche markets of this industry.
A THEORY OF MODULAR EVOLUTION FOR BACTERIOPHAGES *

The modular theory of virus evolution has clear experimental support among the temperate bacteriophages of the enteric bacteria. However, there is also similar genetic and DNA heteroduplex evidence for such evolution among other families of bacteriophages: the virulent bacteriophages of the enterics comprise several families: the T-even group, the T3-T7 group (which has many members among different species of bacteria, including bacteria as widely divergent as E. coli and Caulobacter crescentus. It nicely explains the diffusion of very similar homologous bacteriophages into hosts whose own DNAs have diverged very greatly from each other in nucleotide sequence. It also accounts for the rigorous maintenance of regulatory schemes while units of function (including regions coding for proteins) diverge more rapidly. It should also be noted that the considerations that make modular evolution seem advantageous for bacteriophages apply equally well to viruses of higher organisms. Furthermore, the kinds of heteroduplex similarity observed among animal viruses are reminiscent of what is found for bacteriophages. Viruses found in widely divergent hosts show much greater similarity than would be expected; quite possibly animal viruses also evolve as a population of interchangeable modules.
Cross-desensitization of Chemoattractant Receptors Occurs at Multiple Levels

To define the molecular mechanisms of cross-regulation among chemoattractant receptors, we stably co-expressed, in a rat basophilic leukemia (RBL-2H3) cell line, epitope-tagged receptors for the chemoattractants formylmethionylleucylphenylalanine (fMLP), a peptide of the fifth component of the complement system (C5a), and interleukin-8 (IL-8). All the expressed receptors underwent homologous phosphorylation and desensitization upon agonist stimulation. When co-expressed, epitope-tagged C5a receptor (ET-C5aR) and epitope-tagged IL-8 receptor (ET-IL-8RA) were cross-phosphorylated by activation of the other. Activation of epitope-tagged fMLP receptor (ET-FR) also cross-phosphorylated ET-C5aR and ET-IL-8RA, but ET-FR was totally resistant to cross-phosphorylation. Similarly, C5a and IL-8 stimulation of [35S]guanosine 5′-3-O-(thio)triphosphate (GTPγS) binding and Ca2+ mobilization were cross-desensitized by each other and by fMLP. Stimulation of [35S]GTPγS binding by fMLP was also not cross-desensitized by C5a or IL-8, however, Ca2+ mobilization was, suggesting a site of inhibition distal to G protein activation. Consistent with this desensitization of Ca2+ mobilization, inositol 1,4,5-trisphosphate release in RBL-2H3 cells expressing both ET-C5aR and ET-FR revealed that fMLP and C5a cross-desensitized each other's ability to stimulate phosphoinositide hydrolysis. Taken together, these results indicate that receptor cross-phosphorylation correlates directly with desensitization at the level of G protein activation. The ET-FR was resistant to this process. Of note, cross-desensitization of ET-FR at the level of phosphoinositide hydrolysis and Ca2+ mobilization was demonstrated in the absence of receptor phosphorylation. This suggests a new form of chemoattractant cross-regulation at a site distal to receptor/G protein coupling, involving the activity of phospholipase C.
Central opioidergic neurotransmission in complex regional pain syndrome

Objective: Complex regional pain syndrome (CRPS) is a chronic pain condition characterized by sensory, motor, and autonomic symptoms. It develops after limb trauma and may be associated with relevant psychiatric comorbidity. As there is evidence for central pathophysiology which might be related to an altered opioidergic neurotransmission, we investigated the cerebral opioid receptor status under resting conditions in this patient population. Methods: In this case-control study, 10 patients with CRPS and 10 age- and gender-matched healthy subjects underwent a PET scan using the subtype-nonselective opioidergic radioligand [18F]fluoroethyl-diprenorphine. As a surrogate for regional cerebral opioid receptor availability, the opioid receptor binding potential (OR-BP) was assessed by means of the modified Logan plot with reference region input for categorical group comparison and correlation with clinical data in the patient group. Results: Patients with CRPS showed reduced OR-BP in contralateral amygdala and parahippocampal gyri and increased OR-BP in contralateral prefrontal cortical areas. When OR-BP in the midcingulate cortex and the ipsilateral temporal cortex was low, the McGill pain rating index was high. In general, when anxiety and depression scales were high, contralateral temporal OR-BP was high as well. In addition, the anxiety scale decreased with increasing OR-BP in the contralateral parahippocampal cortex. Conclusions: These results demonstrate altered central opioidergic neurotransmission in CRPS. The correlation of regional opioid receptor availability to measures of pain, anxiety, and depression underlines the clinical importance of these findings.
Quality control of blood irradiation with a teletherapy unit: damage to stored red blood cells after cobalt‐60 gamma irradiation

BACKGROUND: Previous publications have documented the damage caused to red blood cells (RBCs) irradiated with X‐rays produced by a linear accelerator and with gamma rays derived from a 137Cs source. The biologic effects on RBCs of gamma rays from a 60Co source, however, have not been characterized.
Sequence and properties of comQ, a new competence regulatory gene of Bacillus subtilis

The sequence and properties of the comQ gene are described. comQ was predicted to encode a 34,209-Da protein, and the product of comQ was shown to be required for the development of genetic competence. The apparent transcriptional initiation and termination sites of comQ were mapped, and the location of a likely E sigma A promoter was inferred. The expression of comQ was maximal early in growth and declined as the cells approached the stationary phase. This expression was not dependent on any of the competence regulatory genes tested (comA, comP, sin, abrB, degU, and spo0A). Disruption of comQ in the chromosome prevented the development of competence as well as the transcription of comG, a late competence operon. This disruption also decreased the expression of srfA, a regulatory operon needed for the expression of competence. These and other results suggest a role for ComQ early in the hierarchy of competence regulatory genes, probably as a component of a signal transduction system.
Chlamydia species infect human vascular endothelial cells and induce procoagulant activity.

BACKGROUND Chlamydia pneumoniae infections have been linked with myocardial infarction, stroke, and the development of atherosclerosis by epidemiologic studies, immunohistochemical studies, and electron microscopic studies. The mechanisms underlying this association are unknown. METHODS Using cultured human venous endothelial cells, we investigated whether C pneumoniae, C trachomatis (types H and L2/434/BU) could infect these cells. The ability of infected cells to express procoagulant (tissue factor) activity was also measured using clotting and chromogenic substrate assays. Adhesion of platelets to chlamydia-infected cells was also quantitated. RESULTS We found that C pneumoniae, C trachomatis type H, and C trachomatis L2/434/BU could infect cultured human umbilical vein endothelial cells and stimulate a 4-fold increase in expression of tissue factor, which reached a peak 18 hours postinfection. Tissue factor expression was enhanced even in the presence of tetracycline, suggesting that the chlamydial factor responsible for stimulating synthesis of endothelial cell tissue factor was preformed. Platelet adhesion was significantly enhanced when endothelial cells were infected by chlamydia species. CONCLUSIONS These in vitro studies suggest possible pathogenic mechanisms that may explain the association of thrombotic events with C pneumoniae infection, including pathologically enhanced production of tissue factor by human endothelial cells and enhanced focal platelet deposition.
Do Practicing Planners Value Plan Quality? Insights From a Survey of Planning Professionals in Ontario, Canada

Problem, research strategy, and findings: Researchers have developed conceptualconsensus on core plan characteristics that contribute to plan quality, but rarely assess how practitioners view plan quality. I explore how Canadian planners view the characteristics thought to create quality plans. I asked planners in 290 municipalities across southern Ontario (Canada) their view of the importance of plan quality and asked that they identify additional plan quality characteristics, rate the infl uence of various plan characteristics, and explain why they omitted certain characteristics in their offi cial plans. I find that practitioners valued quality plans because they facilitate implementation and help inspire the community while adding credibility and legitimacy to planning processes and the planning profession. Planners did not value all plan elements equally; they felt that clear and enforceable polices were crucial, but that it was less important to report participation in plan preparation or discuss monitoring or interorganizational cooperation in the offi cial plan. Planners did not always include important plan quality elements in their offi cial plans because they lacked suffi cient resources to prepare comprehensive plans and rarely signifi – cantly updated older plans. These case study findings may not be generalizable to other jurisdictions in Canada or internationally, but still offer useful insights. Takeaway for practice: Scholars should ensure that planning practitioners have a key role in shaping understanding of plan quality. Academics and practitioners should work together to create refl ective practitioners who use plan quality characteristics to guide them as they create or update plans. The profession needs to better train current and future planners on how to prepare and evaluate quality plans through improvements in planning curricula and professional development courses offered by the American Planning Association and the Canadian Institute of Planners.
Clinical Implications of New Drugs and Regimens for the Treatment of Drug-resistant Tuberculosis

The emergence of drug-resistant tuberculosis (TB) is a growing problem worldwide. The lack of safe and effective drugs, together with the frequent development of adverse drug reactions can result in worse outcomes. Therefore, new TB drugs able to bolster the current TB treatment regimen are urgently required. Novel drugs that are effective and safe against Mycobacterium tuberculosis are required to reduce the number of drugs and the duration of treatment in both drug-susceptible TB and multi-drug-resistant (MDR)-TB. This review covers promising novel TB drugs and regimens that are currently under development. Bedaquiline and delamanid are the most promising novel drugs for the treatment of MDR-TB, each having a high efficacy and tolerability. However, the best regimen for achieving better outcomes and reducing adverse drug reactions remains yet to be determined, with safety concerns regarding cardiac events due to QT prolongation still to be addressed. Pretomanid is a novel drug that potentially shortens the duration of treatment in both drug-susceptible and drug-resistant TB. Many regimens consisting of injection free drugs with shorter treatment duration compared to the conventional treatment are now undergoing clinical trials. Therefore a simple and short treatment with higher efficacy, and lesser adverse drug reactions and drug-drug interaction is expected for patients with MDR-TB.
Regulation of Protein Arginine Methyltransferase 8 (PRMT8) Activity by Its N-terminal Domain*

Human protein arginine methyltransferase PRMT8 has been recently described as a type I enzyme in brain that is localized to the plasma membrane by N-terminal myristoylation. The amino acid sequence of human PRMT8 is almost 80% identical to human PRMT1, the major protein arginine methyltransferase activity in mammalian cells. However, the activity of a recombinant PRMT8 GST fusion protein toward methyl-accepting substrates is much lower than that of a GST fusion of PRMT1. We show here that both His-tagged and GST fusion species lacking the initial 60 amino acid residues of PRMT8 have enhanced enzymatic activity, suggesting that the N-terminal domain may regulate PRMT8 activity. This conclusion is supported by limited proteolysis experiments showing an increase in the activity of the digested full-length protein, consistent with the loss of the N-terminal domain. In contrast, the activity of the N-terminal truncated protein was slightly diminished by limited proteolysis. Significantly, we detect automethylation at two sites in the N-terminal domain, as well as binding sites for SH3 domain-containing proteins. We suggest that the N-terminal domain may function as an autoregulator that may be displaced by interaction with one or more physiological inducers.
Optimization and development of concurrent EEG-fMRI data acquisition setup for understanding neural mechanisms of brain

Electroencephalography (EEG) and functional magnetic resonance (fMRI) both are considered as non-invasive neuroimaging modalities. Both are used for understanding brain functionalities in cognitive neuroscience as well as in clinical applications. EEG gives high temporal resolution and it has poor spatial resolution. On the other hand, fMRI has very high spatial resolution and poor temporal resolution. For deep understanding of neural mechanisms inside human brain, it is desirable to get the higher spatiotemporal resolution of human brain at the same time. Concurrent EEG-fMRI data recording solve the problem of higher spatiotemporal resolution. It can be also helpful to understand the neural mechanism inside human brain effectively. The concurrent EEG-fMRI recording requires MRI compatible EEG equipment which can be placed inside the higher magnetic field of MRI scanner and also synchronization is required to make setup concurrent. To get higher signal to noise ratio (SNR), optimization of data acquisition parameters plays a significant role. In this paper, we discussed the some real issues during data acquisition and their optimization. We have developed the concurrent EEG-fMRI setup and also successfully recorded the EEG-fMRI data concurrently by optimizing the data acquisition parameters involved. Artifacts have been removed from the data and further, data fusion framework is proposed for combine analysis of EEG and fMRI data.
Crystal Structure of Get4-Get5 Complex and Its Interactions with Sgt2, Get3, and Ydj1*♦

Get3, Get4, and Get5 in Saccharomyces cerevisiae participate in the insertion of tail-anchored proteins into the endoplasmic reticulum membrane. We elucidated the interaction between Get4 and Get5 and investigated their interaction with Get3 and a tetratricopeptide repeat-containing protein, Sgt2. Based on co-immunoprecipitation and crystallographic studies, Get4 and Get5 formed a tight complex, suggesting that they constitute subunits of a larger complex. In contrast, although Get3 interacted physically with the Get4-Get5 complex, low amounts of Get3 co-precipitated with Get5, implying a transient interaction between Get3 and Get4-Get5. Sgt2 also interacted with Get5, although the amount of Sgt2 that co-precipitated with Get5 varied. Moreover, GET3, GET4, and GET5 interacted genetically with molecular chaperone YDJ1, suggesting that chaperones might also be involved in the insertion of tail-anchored proteins.
Catch 22 faculty tenure for public universities: new evidence employing Färe–Primont productivity indexes

ABSTRACT With decreases in funding support, U.S. public universities have increasingly substituted less costly nontenure eligible faculty for tenured and tenure-track faculty while newly imposed funding mandates require evidence of productivity improvements tied to student graduation success. Universities have been forced into a Catch 22 dilemma as to what faculty mix produces optimal student/funding outcomes. To date, guidance provided by empirical evidence is mixed. This article offers new evidence employing Färe–Primont productivity indexes and parametric estimates to determine how faculty status affects graduation productivity. Results indicate that a reversal in the employment trend is in order so as to increase tenured relative to nontenure eligible faculty and improve student graduation success and, ultimately, university funding.
VIN6: VIN-based IPv6 provider independent addressing for future vehicular internet communications

Recent years witnessed growing concerns regarding scalability of today's Internet architecture. Universality of the single IP numbering space is one cause of unbound growth of entropy and inter-domain routing tables in the core network. Recently, the IAB proposed to review the Internet addressing architecture to separate name and location spaces. In this paper, we propose an instance of VIN-based IPv6 addressing, compatible with other Future Internet efforts, to tackle this issue with regards to the vehicular communications. We design a global hierarchical identification space of up to 235 unique and distinct vehicles per manufacturer (216 manufacturers). An original method of mapping these identifiers to addresses and guarantee the uniqueness conservation is proposed. The objective is to create a sustainable addressing architecture for vehicular communications and introduce the manufacturer as a new vehicular services provider. We also propose a network architecture design that allows to use pseudonyms as a way to tackle identity privacy concerns.