Miracle-dz/UniFAR-FTUs huggingface dataset:
Optimizing Child Nutrition Education With the Foodbot Factory Mobile Health App: Formative Evaluation and Analysis

Background Early nutrition interventions to improve food knowledge and skills are critical in enhancing the diet quality of children and reducing the lifelong risk of chronic disease. Despite the rise of mobile health (mHealth) apps and their known effectiveness for improving health behaviors, few evidence-based apps exist to help engage children in learning about nutrition and healthy eating. Objective This study aimed to describe the iterative development and user testing of Foodbot Factory, a novel nutrition education gamified app for children to use at home or in the classroom and to present data from user testing experiments conducted to evaluate the app. Methods An interdisciplinary team of experts in nutrition, education (pedagogy), and game design led to the creation of Foodbot Factory. First, a literature review and an environmental scan of the app marketplace were conducted, and stakeholders were consulted to define the key objectives and content of Foodbot Factory. Dietitian and teacher stakeholders identified priority age groups and learning objectives. Using a quasi-experimental mixed method design guided by the Iterative Convergent Design for Mobile Health Usability Testing approach, five app user testing sessions were conducted among students (ages 9-12 years). During gameplay, engagement and usability were assessed via direct observations with a semistructured form. After gameplay, qualitative interviews and questionnaires were used to assess user satisfaction, engagement, usability, and knowledge gained. Results The environmental scan data revealed that few evidence-based nutrition education apps existed for children. A literature search identified key nutrients of concern for Canadian children and techniques that could be incorporated into the app to engage users in learning. Foodbot Factory included characters (2 scientists and Foodbots) who initiate fun and engaging dialogue and challenges (minigames), with storylines incorporating healthy eating messages that align with the established learning objectives. A total of five modules were developed: drinks, vegetables and fruit, grain foods, animal protein foods, and plant protein foods. Seven behavior change techniques and three unique gamified components were integrated into the app. Data from each user testing session were used to inform and optimize the next app iteration. The final user testing session demonstrated that participants agreed that they wanted to play Foodbot Factory again (12/17, 71%), that the app is easy to use (12/17, 71%) and fun (14/17, 88%), and that the app goals were clearly presented (15/17, 94%). Conclusions Foodbot Factory is an engaging and educational mHealth intervention for the Canadian public that is grounded in evidence and developed by an interdisciplinary team of experts. The use of an iterative development approach is a demonstrated method to improve engagement, satisfaction, and usability with each iteration. Children find Foodbot Factory to be fun and easy to use, and can engage children in learning about nutrition.
Interdigitation Zone Change According to Glaucoma-Stage Advancement

Purpose To compare the macular interdigitation zone (IZ) of normal eyes with eyes showing different normal-tension glaucoma (NTG) stages. Methods Forty-two normal eyes (age, 56 ± 5.4 years), 45 pre-perimetric eyes (age, 59 ± 6.9 years), 51 mild-to-moderate glaucoma eyes (age, 58 ± 7.2 years; mean deviation [MD], –5.5 ± 3.0 dB), and 50 severe glaucoma eyes (age, 59 ± 6.9 years; MD, –15.1 ± 5.4 dB) were enrolled. All of the subjects underwent high-resolution spectral-domain optical coherence tomography (SD-OCT) to obtain 19 horizontal and 19 vertical macular B-scans 9 mm in length. The en face image of the scan area was divided into 589 rectangular boxes (side length of 375 µm). The IZ locations were marked on the corresponding image boxes. The IZ area was then quantified according to the number of boxes showing IZs among the 589 total boxes. Results The IZ area in the severe glaucoma eyes was significantly smaller than in the mild-to-moderate glaucoma eyes (28.99 ± 7.88 mm2 vs. 40.79 ± 7.46 mm2; P < 0.001), was smaller in the mild-to-moderate glaucoma eyes than in the pre-perimetric glaucoma eyes (40.79 ± 7.46 mm2 vs. 49.92 ± 8.10 mm2; P < 0.001), and was smaller still in the pre-perimetric glaucoma eyes than in the normal eyes (49.92 ± 8.10 mm2 vs. 56.85 ± 7.94 mm2; P < 0.001). In the 146 NTG eyes, a statistically significant correlation was found between IZ area and MD (r = 0.64; P < 0.001). Conclusions SD-OCT revealed a reduction in IZ area in NTG eyes, and the extent of the reduction was positively associated with glaucoma severity. These findings suggest, though tentatively, that changes in the outer retinal layer can occur in the course of glaucoma progression.
INTRODUÇÃO DE NOVOS GERMOPLASMAS DE MILHO NO BRASIL

In 1976 many sources of new germplasm considered to have potential in Brazil were selected and introduced from CIMMYT. This material was evaluated in 51 experiments throughout Brazil in a network of national trials to determine its genetic potential. On the basis of these results, the following germplasm have potential in Brazil: Pool 21, 22, 25 and 26, Suwan DMR, Tuxpeno 1, Mezcla Tropical Blanco, Blanco Cristalino 1, Antigua x Vera Cruz 181, Mezcla Amarilla, Amarillo Cristalino, Amarillo Dentado and Subtropical, Antigua x Rep. Dominicana and Eto x Illinois. They were selected based upon high mean yield, earliness and low plant and ear height, except for Pools and Suwan DMA which were only considered for high mean yield.
Detection of novel and confirmation of very rare and rare HLA alleles by next generation sequencing in Croatia

Routine HLA typing in clinical practice encompassing solid organ and hematopoietic stem cells transplantation programs, disease association typing, volunteer marrow donor typing and population studies, provided a large dataset for studying HLA allele polymorphism in the Croatian population which led to the identification of new, very rare and rare HLA alleles. Over the last 4 years we have identified six new HLA alleles (HLA‐A*01:200, A*02:836, A*11:01:01:44, B*08:251, B*18:169 and C*05:46:01:02) and a number of very rare (HLA‐B*08:78, DRB1*12:39, DRB1*13:23:02 and DQB1*06:09:04) or rare (HLA‐A*24:41, B*39:40:01N, B*51:78:01, DRB1*01:31 and DRB1*14:111) alleles using sequence‐based typing methods. The reported data enhance the knowledge about HLA polymorphisms in the Croatian population and provide a foundation for further studies in population genetics.
Complete Genome Sequence of Halomonas meridiana Strain Slthf1, Isolated from a Deep-Sea Thermal Vent

Halomonas meridiana strain Slthf1 (ATCC BAA-801) is a Gram-negative bacterium that was isolated from a thermal vent in 1998. Here, we report the complete genome sequence of this strain, which has a 3.6-Mbp genome, containing 3,400 protein-coding sequences. ABSTRACT Halomonas meridiana strain Slthf1 (ATCC BAA-801) is a Gram-negative bacterium that was isolated from a thermal vent in 1998. Here, we report the complete genome sequence of this strain, which has a 3.6-Mbp genome, containing 3,400 protein-coding sequences.
Reweighting Randomized Controlled Trial Evidence to Better Reflect Real Life – A Case Study of the Innovative Medicines Initiative

Evidence from randomized controlled trials available for timely health technology assessments of new pharmacological treatments and regulatory decision making may not be generalizable to local patient populations, often resulting in decisions being made under uncertainty. In recent years, several reweighting approaches have been explored to address this important question of generalizability to a target population. We present a case study of the Innovative Medicines Initiative to illustrate the inverse propensity score reweighting methodology, which may allow us to estimate the expected treatment benefit if a clinical trial had been run in a broader real‐world target population. We learned that identifying treatment effect modifiers, understanding and managing differences between patient characteristic data sets, and balancing the closeness of trial and target patient populations with effective sample size are key to successfully using this methodology and potentially mitigating some of this uncertainty around local decision making.
Bridge-Enhanced Anterior Cruciate Ligament Repair Is Not Inferior to Autograft Anterior Cruciate Ligament Reconstruction at 2 Years: Results of a Prospective Randomized Clinical Trial

Background: Preclinical studies suggest that for complete midsubstance anterior cruciate ligament (ACL) injuries, a suture repair of the ACL augmented with a protein implant placed in the gap between the torn ends (bridge-enhanced ACL repair [BEAR]) may be a viable alternative to ACL reconstruction (ACLR). Hypothesis: We hypothesized that patients treated with BEAR would have a noninferior patient-reported outcomes (International Knee Documentation Committee [IKDC] Subjective Score; prespecified noninferiority margin, –11.5 points) and instrumented anteroposterior (AP) knee laxity (prespecified noninferiority margin, +2-mm side-to-side difference) and superior muscle strength at 2 years after surgery when compared with patients who underwent ACLR with autograft. Study Design: Randomized controlled trial; Level of evidence, 1. Methods: One hundred patients (median age, 17 years; median preoperative Marx activity score, 16) with complete midsubstance ACL injuries were enrolled and underwent surgery within 45 days of injury. Patients were randomly assigned to receive either BEAR (n = 65) or autograft ACLR (n = 35 [33 with quadrupled semitendinosus-gracilis and 2 with bone–patellar tendon–bone]). Outcomes—including the IKDC Subjective Score, the side-to-side difference in instrumented AP knee laxity, and muscle strength—were assessed at 2 years by an independent examiner blinded to the procedure. Patients were unblinded after their 2-year visit. Results: In total, 96% of the patients returned for 2-year follow-up. Noninferiority criteria were met for both the IKDC Subjective Score (BEAR, 88.9 points; ACLR, 84.8 points; mean difference, 4.1 points [95% CI, –1.5 to 9.7]) and the side-to-side difference in AP knee laxity (BEAR, 1.61 mm; ACLR, 1.77 mm; mean difference, –0.15 mm [95% CI, –1.48 to 1.17]). The BEAR group had a significantly higher mean hamstring muscle strength index than the ACLR group at 2 years (98.2% vs 63.2%; P < .001). In addition, 14% of the BEAR group and 6% of the ACLR group had a reinjury that required a second ipsilateral ACL surgical procedure (P = .32). Furthermore, the 8 patients who converted from BEAR to ACLR in the study period and returned for the 2-year postoperative visit had similar primary outcomes to patients who had a single ipsilateral ACL procedure. Conclusion: BEAR resulted in noninferior patient-reported outcomes and AP knee laxity and superior hamstring muscle strength when compared with autograft ACLR at 2-year follow-up in a young and active cohort. These promising results suggest that longer-term studies of this technique are justified. Registration: NCT02664545 (ClinicalTrials.gov identifier)
Cannabis-Infused Edible Products in Colorado: Food Safety and Public Health Implications.

Cannabis-infused "edibles" are a popular means of cannabis use, and the variety of edible food products available to consumers continues to grow. Although there has been much discussion on dose standardization, childproof packaging, and the prevention of overconsumption, the important topic of food safety has received less attention.We discuss potential food safety hazards associated with cannabis-infused edible food products, drawing on examples from Colorado, and describe edible-associated foodborne illness outbreaks and other contamination events.It is important for public health agencies, particularly environmental health and enteric disease programs, to be familiar with the cannabis industry, including regulatory partners, signs and symptoms of cannabis ingestion, the scope of edible products sold and consumed, and the food safety risks unique to cannabis products. (Am J Public Health. Published online ahead of print April 16, 2020: e1-e6. doi:10.2105/AJPH.2020.305601).
Spatially Dissociated Intracerebral Maps for Face- and House-Selective Activity in the Human Ventral Occipito-Temporal Cortex.

We report a comprehensive mapping of the human ventral occipito-temporal cortex (VOTC) for selective responses to frequency-tagged faces or landmarks (houses) presented in rapid periodic trains of objects, with intracerebral recordings in a large sample (N = 75). Face-selective contacts are three times more numerous than house-selective contacts and show a larger amplitude, with a right hemisphere advantage for faces. Most importantly, these category-selective contacts are spatially dissociated along the lateral-to-medial VOTC axis, respectively, consistent with neuroimaging evidence. At the minority of "overlap" contacts responding selectively to both faces and houses, response amplitude to the two categories is not correlated, suggesting a contribution of distinct populations of neurons responding selectively to each category. The medio-lateral dissociation also extends into the underexplored anterior temporal lobe (ATL). In this region, a relatively high number of intracerebral recording contacts show category-exclusive responses (i.e., without any response to baseline visual objects) to faces but rarely to houses, in line with the proposed role of this region in processing people-related semantic information. Altogether, these observations shed novel insight on the neural basis of human visual recognition and strengthen the validity of the frequency-tagging approach coupled with intracerebral recordings in epileptic patients to understand human brain function.
Temporal Variations in a Tropical Soft-Bottom Community, Venezuela

The relationship between macroinfaunal density fluctuations and temporal variations of environmental parameters were studied. Physico-chemical, sediment, and biological parameters from a tropical soft-bottom community were followed monthly during a 2 1/2 years survey, and their possible relationships were elucidated using multivariate analyses. The community at the study site was composed of 37% polychaetes, 21% crustaceans (mainly amphipods), 15% nematodes, 5% oligochaetes and 22% shared among gastropods, bivalves, and other minor groups. Results showed little temporal variation for environmental and sediment variables, except for the grain-size fractions, and clear fluctuations in macroinfaunal densities. Polychaete total abundances showed two annual peaks, one during the summer months (mainly June to July, but May and July in 1991) and a second one towards the end of the year (November to December). Crustaceans, the second major group, showed only one annual peak, either early (April-May 1990 and 1991) or late summer (July-August 1989). Similar temporal density variations were also noted for the nematodes, with one annual peak during June and July (but exceptionally high values in February 1990 and 1991) and lowest values in April 1990 and January 1991. The molluscs (gastropods and bivalves) occurred with very low densities and showed no clear temporal trend. Polychaetes, the dominant group, were represented by 22 species belonging to 15 families, with spionids, paraonids, and capitellids as the families with the highest richness. Maximum species richness was obtained in October 1990 and April 1991 and minimum richness in July 1990 and January 1991, with 10 polychaete species (± 2 species) as a monthly average. In terms of abundance, 21% of the polychaetes were represented by Lumbrineris tetraura , 10% by Prionospio pygmaea , 5% by the cirratulid Tharyx sp . , 5% by Magelona pettiboneae , and less than 2% for the rest of the species of this group. Lumbrineris tetraura , the dominant species, had two annual density peaks per year except in 1990, where no peak for the summer months was evident. Prionospio pygmaea had the highest densities during September-October 1989 and the lowest during April, June, and August 1991. Maximal values were also observed for Tharyx sp. in November-December 1989, and during April, June, and July of the same year for Magelona pettiboneae . Results of the ordination analyses by CCA revealed little association between faunal densities and sediment parameters suggesting that other factors may be responsible for the macroinfaunal density patterns at the study site, such as factors related to biological interactions between species or factors extrinsic to the community, related to the input of external pulses of organic material. We propose that macroinfaunal density peaks may be a consequence of a combination of the above factors at our study site.
Exploring the impact of safety behaviour use on cognitive, psychophysiological, emotional and behavioural responses during a speech task

Abstract Background: There is a debate among researchers and clinicians regarding whether the judicious use of safety behaviours (SBs) during exposure therapy is helpful or detrimental. Central to this debate is the premise that SBs may interfere with one’s ability to gather disconfirmatory evidence. Aims: No study to date has assessed how SB use may impact cognitive mechanisms implicated during an exposure-like task. We investigated multiple cognitive, emotional, psychophysiological and behavioural underpinnings of exposure with and without SBs. Method: Speech anxious participants (n = 111) were randomly assigned to deliver an evaluated speech with or without SBs. Self-reported anxiety ratings and psychophysiological arousal measures were recorded at baseline, in anticipation of the speech, and following the speech. Measures of working memory, ability to gather disconfirmatory evidence, speech duration, objective and subjective speech performance, and speech task acceptability were administered. Results: There were no differences between conditions on working memory, self-reported anxiety, psychophysiological arousal, ability to gather disconfirmatory evidence, speech duration, or objective and subjective speech performance. All participants were able to gather disconfirmatory evidence. However, condition did influence willingness to deliver future speeches. Our sample was largely female undergraduate students, and we offered only a small number of specific safety behaviours. Conclusions: Judicious SB use may not necessarily be detrimental, but clients may believe them to be more helpful than they actually are.
Mouse γ-Synuclein Promoter-Mediated Gene Expression and Editing in Mammalian Retinal Ganglion Cells

Optic neuropathies are a group of optic nerve (ON) diseases caused by various insults including glaucoma, inflammation, ischemia, trauma, and genetic deficits, which are characterized by retinal ganglion cell (RGC) death and ON degeneration. An increasing number of genes involved in RGC intrinsic signaling have been found to be promising neural repair targets that can potentially be modulated directly by gene therapy, if we can achieve RGC specific gene targeting. Optic neuropathies are a group of optic nerve (ON) diseases caused by various insults including glaucoma, inflammation, ischemia, trauma, and genetic deficits, which are characterized by retinal ganglion cell (RGC) death and ON degeneration. An increasing number of genes involved in RGC intrinsic signaling have been found to be promising neural repair targets that can potentially be modulated directly by gene therapy, if we can achieve RGC specific gene targeting. To address this challenge, we first used adeno-associated virus (AAV)-mediated gene transfer to perform a low-throughput in vivo screening in both male and female mouse eyes and identified the mouse γ-synuclein (mSncg) promoter, which specifically and potently sustained transgene expression in mouse RGCs and also works in human RGCs. We further demonstrated that gene therapy that combines AAV-mSncg promoter with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing can knock down pro-degenerative genes in RGCs and provide effective neuroprotection in optic neuropathies. SIGNIFICANCE STATEMENT Here, we present an RGC-specific promoter, mouse γ-synuclein (mSncg) promoter, and perform extensive characterization and proof-of-concept studies of mSncg promoter-mediated gene expression and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing in RGCs in vivo. To our knowledge, this is the first report demonstrating in vivo neuroprotection of injured RGCs and optic nerve (ON) by AAV-mediated CRISPR/Cas9 inhibition of genes that are critical for neurodegeneration. It represents a powerful tool to achieve RGC-specific gene modulation, and also opens up a promising gene therapy strategy for optic neuropathies, the most common form of eye diseases that cause irreversible blindness.
Characterisation of A Maltogenic Alpha Amylase ofAspergillus Niger and Studies on The Enzyme Overproducing Mutant

Characterisation of a 53 kDa maltogenic α-amylase from the culture filtrate of A. niger is new to literature. Production of this enzyme by the fungus could be improved by UV mutation and temperature selection. Studies using the maltogenic α-amylase over producing mutant showed that high molecular mass precursor starch hydrolysing enzyme is degraded to G1 and G2 forms of glucoamylase and maltogenic α-amylase by autocatalysis in order to overcome secretional difficulties associated with reduced cell wall porosity to high molecular mass proteins. Standardization of fermentation conditions to a pilot plant scale described a process for the enzyme production by submerged fermentation. In defining the media components for maltogenic α-amylase production, acarbose as a screening reagent appeared effective since it inhibited all other amylases.
Draft Genome Sequences of Seven Streptococcus canis Strains Isolated from Diseased Companion Animals in Japan

The draft genomes of seven Streptococcus canis isolates from diseased dogs and cats in Japan are reported here. The genome sizes ranged from 1.901 Mbp to 2.203 Mbp, with G+C contents of 39.5% to 39.8%. The sequence types, antimicrobial resistance genotypes, and S. canis M-like protein alleles were all characterized. ABSTRACT The draft genomes of seven Streptococcus canis isolates from diseased dogs and cats in Japan are reported here. The genome sizes ranged from 1.901 Mbp to 2.203 Mbp, with G+C contents of 39.5% to 39.8%. The sequence types, antimicrobial resistance genotypes, and S. canis M-like protein alleles were all characterized.
Strong Metal-Support Interactions between Pt Single Atoms and TiO2.

Strong metal-support interaction (SMSI) has gained great attention in heterogeneous catalysis field. However, whether single - atom catalyst can exhibit SMSI remains unknown. Here, we demonstrated that the SMSI can occur on TiO 2 supported Pt single atoms but at a much higher reduction temperature than that on Pt nanoparticles (NPs). In SMSI state Pt single atoms neither are covered by, nor sink into the subsurface of, TiO 2 support. The suppression of CO adsorption on Pt single atoms stems from the coordination saturation (18 electron role) rather than the physical coverage by support. Based on the new finding it is revealed that in 3-nitrostyrene hydrogenation reaction single atoms are the real active sites while Pt NPs barely contribute to the activity through selectively encapsulating the NP sites. The finding in this work provides a new approach to study the active sites by tuning the SMSI state.