Miracle-dz/UniFAR-FTUs huggingface dataset:
Converging Pathways in the Regulation of Longevity and Metabolism in Caenorhabditis Elegans: A Dissertation

The lifespan of an organism is determined by a complex array of genetic, environmental and nutritional factors. Yet single gene manipulations have been shown to significantly extend lifespan in several model organisms. Of all the genes that have been studied thus far, components of the insulin/IGF-1 signaling (IIS) pathway have emerged as the most robust regulators of longevity. In addition, IIS also regulates development, energy metabolism and the response to stress in a conserved manner. In Caenorhabditis elegans , signaling through this pathway is initiated by activation of the insulin/IGF-1 receptor tyrosine kinase DAF-2, which then activates a PI3-kinase signaling pathway involving additional downstream serine/threonine kinases such as PDK-1, AKT-1, AKT-2 and SGK-1. The concerted action of these kinases results in the negative regulation of the single FOXO transcription factor homolog DAF-16. Under reduced signaling conditions, active DAF-16 is able to translocate into the nucleus and regulate the expression of hundreds of genes regulating longevity, stress resistance, metabolism and development. The PTEN phosphatase homolog DAF-18, which antagonizes IIS at the level of PI3-kinase, is a major negative regulator of the pathway. However, not much was known about additional phosphatases that negatively regulated the kinases in the pathway. Dephosphorylation is a critical regulatory mechanism by which cellular signaling homeostasis is maintained. Aberrant hyper-activation of growth factor signaling pathways, including IIS, has been implicated in several cancers. In addition, deregulation of IIS is also closely linked to Type II diabetes. Therefore, the identification phosphatases that balance kinase activity will provide a better understanding of the regulation of the IIS pathway under normal as well as disease conditions. A directed RNAi screen using dauer diapause was conducted in our lab to identify serine/threonine phosphatases that modulated IIS. My work in the Tissenbaum Lab has primarily focused on characterization of the top three candidates from this screen, the genes pptr-1 , pdp-1 and fem-2 . From these studies, we have also uncovered novel crosstalk between the IIS and TGF-β signaling pathways. In Chapter 2, we demonstrate that PPTR-1, a PP2A phosphatase regulatory subunit negatively regulates the IIS pathway by modulating AKT-1 dephosphorylation. PPTR-1 modulates several outputs of IIS similar to DAF-18. In addition, PPTR-1 co-localizes and physically interacts with its substrate, AKT-1. PPTR-1 modulates dephosphorylation of AKT-1 at a conserved threonine site and we show the molecular conservation of this interaction in mammalian adipocytes. Ultimately, this negative regulation by PPTR-1 results in increased DAF-16 nuclear localization and transcriptional activity. Next, in Chapter 3, we show how PDP-1 is a novel link between the IIS and TGF-β signaling pathways. Similar to DAF-18 and PPTR-1, PDP-1 regulates multiple outputs of the IIS pathway…
La perception des séquences consonantiques non-natives par les locuteurs monolingues de mandarin (Perception of non-native consonant sequences by Mandarin monolingual speakers)[In French]

Cette étude examine le rôle de la structure phonotactique native et des facteurs phonétiques dans la perception des séquences consonantiques non-natives. Des locuteurs monolingues de mandarin ont été testés dans les deux expériences suivantes: dans la première expérience, les locuteurs ont du décider s’ils entendaient une voyelle entre deux consonnes en écoutant des séquences intervocaliques-CC (akta) et leurs contrôles CVC (akata). Les participants mandarins monolingues ont tendance à percevoir une voyelle entre deux consonnes dans les deux séquences CC et CVC. Mais le pourcentage de la voyelle perçue varie selon les différentes séquences. Dans la deuxième expérience, les mêmes participants ont écouté des séquences CC initiales et intervocaliques (ktapa, akta) ainsi que CVC (katapa, akata) et les ont transcrites en Pinyin. Les stratégies observées dans la transcription: l’épenthèse, la métathèse, l’omission de C1 et celle de C2, montrent que les participants sont sensibles aux facteurs phonétiques. Les résultats des deux expériences suggèrent que la phonotactique native ainsi que des facteurs phonétiques affectent la perception des séquences non-natives.
Cryptic marine biota of the Raja Ampat island group : Preliminary results of the Raja Ampat Expedition (2007), Ekspedisi Widya Nusantara (E-Win) of the Indonesian Institute of Sciences (LIPI)

The Raja Ampat expedition in 2007 was organized by the National Museum of Natural History Naturalis in Leiden in cooperation with the Research Centre for Oceanography (RCO-LIPI, Jakarta) as part of Ekspedisi Widya Nusantara (E-Win expedition) of the Indonesian Institute of Sciences (LIPI). The research team consisted of scientists from Indonesia, the Netherlands, Palau and the United States. The Raja Ampat islands are located northwest of Bird’s Head' peninsula in Papua, Indonesia. This area has recently gained much media attention due to its high marine biodiversity and the recent discovery of several new species of corals and fish. The expedition was based at Kri Eco Resort of Papua Diving, on Kri Island. The expedition aimed to study cryptic biota, which consist of species that lead hidden lives. They may be difficult to find because of their small size, their successful camouflage or mimicry, or because they live in habitats that are nearly inaccessible or easily overlooked. Some species resemble phylogenetically related species, called sibling species, which can be detected with the help of molecular (DNA) analyses. Use of camouflage is seen in many animals that live in close association with a host. In the case of such interspecific associations, the expedition members also studied the host organisms. Marine lakes, deep land inward bays, narrow channels, and deep sandy bottoms underneath reef slopes are examples of habitats that were studied despite their limited accessibility. All together, many different marine environments were studied.
Nest-Site Selection in the Eastern Box Turtle, Terrapene carolina carolina, in a Population in Central Illinois

Nest site selection in turtles might allow females to increase the survival of their offspring but little is known about the variables that influence that choice. I examined the microhabitat variables at nest sites of the eastern box turtle, Terrapene carolina carolina, at a Boy Scout camp in Shelby County, Illinois, to determine if females chose nest sites that differed from random sites within the camp. Nest sites differed from random sites in vegetation height and composition, percentage ground and canopy cover, and light intensity. All females placed their nests in open habitat within the camp or a pasture bordering the camp. I suggest that, due to the importance of incubation temperature on hatchling development, open sites were selected due to the higher incubation temperatures they provide.
RNA-protein crosslinking identifies novel targets for the nuclear RNA surveillance machinery

The RNA binding proteins Nrd1 and Nab3 function in transcription termination by RNA Pol II, acting via interactions with the CTD of the largest polymerase subunit, particularly on snRNA and snoRNA genes. They also participate in nuclear RNA surveillance and ncRNA degradation, functioning together with the exosome and the Trf-Air-Mtr4 polyadenylation (TRAMP) complexes. To better understand the signals for surveillance and ncRNA degradation, I applied an RNA-protein crosslinking approach in combination with Solexa sequencing. This approach identified in vivo binding sites for Nrd1, Nab3 and Trf4. Several million sequences were recovered and mapped to the yeast genome. This identified three classes of substrates: 1) Expected targets, including snRNAs, snoRNAs and characterized short ncRNAs. 2) Unknown but anticipated substrates, including several hundred previously uncharacterized ncRNAs that lie antisense to protein coding genes (asRNAs). 3) Unexpected targets, including many Pol III transcribed precursor RNAs. Bioinformatics analyses of the high-throughput sequencing data revealed that known binding motifs for Nrd1 and Nab3 were frequently recovered. Many recovered RNAs contained non-templated oligo(A) tails with an average of 2-5 nt length. This clearly distinguishes targets for surveillance machinery from polyadenylated mRNAs that get stabilized by polyadenylation (A70-90 in yeast). For a few selected, predicted asRNAs I was able to validate the crosslinking data by demonstrating that corresponding long RNAs are both detectable and increased by loss of Nrd1, Nab3, Trf4 or the exosome component Rrp6. Interestingly, loss of Nrd1 or Nab3 led to transcriptional read through on long asRNA transcripts. In addition, I have identified pre-TLC1 (telomerase RNA) as a target for the surveillance machinery. Processing of this long ncRNA was only poorly characterized in yeast but I could demonstrate that its transcription termination and maturation is mainly dependent on actions of the Nrd1-Nab3-Sen1, TRAMP4 and exosome complexes. It was previously reported that Nrd1-Nab3 acts only on short RNAs, due to the association with Ser5 phosphorylated CTD. My findings suggest that action of Nrd1Nab3 is not exclusively on Ser5 phosphorylated form of the CTD. Unexpectedly the Pol II associated factors Nrd1 and Nab3 bound Pol III precursor transcripts. Surveillance of Pol III transcripts was dependent on Nrd1 and Nab3 since depletion of Nrd1 or Nab3 led to accumulation of pre-tRNAs. In addition, I could demonstrate that pre-RNase P RNA is oligoadenylated in vivo, which was dependent on Nrd1, Nab3 and Trf4. Together, my findings suggest a revised model of nuclear RNA surveillance in which Nrd1-Nab3 not only acts in co-transcriptional RNA recognition on Pol II transcripts but also post-transcriptionally on Pol III RNAs. The TRAMP complex is recruited to the defective RNA by the Nrd1-Nab3 complex, which remains associated with the RNA through the process of polyadenylation, until the exosome degrades the aberrant transcript.
Endothelins in Biology and Medicine

Endothelin Receptors Endothelin Receptors: Pharmacological Subtyping, Agonists and Antagonists, C.R. Hiley Endothelin Receptor Molecular Biology, J.P. Huggins Distribution of Endothelin Receptors, A.P. Davenport Peptide Receptor Interactions, D. Guedin, C. Frelin, and P. Vigne Endothelin-Induced Intracellular Signaling Pathways, H. Schramek and M.J. Dunn Endothelin Synthesis and Degradation Endothelin Converting Enzymes and Related Inhibitors, M. Biguad and H. D'Orchymont Endothelin Metabolism, S. Kaw and M. Yanagisawa Endothelin Pathophysiology Cardiovascular Effects of Endothelin, B. Gumusel, Q. Hao, J.K. Chang, A. Hyman, and H. Lippton Effects of Endothelin in the Kidney, P. Nambi and D.P. Brooks Endothelin-Induced Responses in the Pulmonary System, B. Battistini and T.D. Warner Effects of Endothelins on the Nervous System, M. van den Buuse Urogenital Actions of Endothelins, K.S. Sang and J.P. Huggins Endothelin in the Eye, N.N. Osborne Ligand Structure and Activity Relationships Conformational Studies of Endothelins and Analogs, J.T. Pelton Endothelin Agonists, J.T. Pelton Development of Endothelin Agonists, A.M. Doherty and W.C. Patt Clinical Studies Clinical Pharmacology and Pathophysiology of the Endothelins, W.G. Haynes and D.J. Webb
National Association of School Psychologists Principles for Professional Ethics

I. Respecting the Dignity and Rights of All Persons School psychologists engage only in professional practices that maintain the dignity of all individuals. In their words and actions, school psychologists demonstrate respect for the autonomy of persons and their right to self-determination, respect for privacy, and a commitment to just and fair treatment of all persons. Principle I.1. Autonomy and Self-Determination (Consent and Assent) Principle I.2. Privacy and Confidentiality Principle I.3. Fairness and Justice
Gluthione transferases and gamma-glutamyl transpeptidases

By reading, you can know the knowledge and things more, not only about what you get from people to people. Book will be more trusted. As this glutathione transferases and gamma glutamyl transpeptidases, it will really give you the good idea to be successful. It is not only for you to be success in certain life you can be successful in everything. The success can be started by knowing the basic knowledge and do actions.
Two Cases of Isolated ACTH Deficiency Presenting As Hyponatremia - Case Report

Non-specific symptoms such as asthenia, anorexia, unintentional weight loss, nausea/vomiting particularly in the elderly population are often overlooked both by caretakers and physicians. Deteriorating general health and neuro-psychiatric symptoms are often attributed to depression in the elderly population. The most common electrolyte abnormality, hyponatremia is again more common in the elderly population. Often neglected chronic hyponatremia remains asymptomatic in many cases. Once hyponatremia is detected clinicians should establish a proper diagnosis before supplementation. Failure to diagnose such cases will lead to repeated hospitalization, poor quality of life, wastage of resources and even death. Isolated ACTH deficiency (IAD) is a rare disorder and potentially fatal. IAD can present rarely as hyponatremia and diagnosis can easily be missed if not suspected. IAD may not be as rare as earlier thought as more and more such cases are been reported. Here we report two cases of generalized weakness and nausea; they were repeatedly hospitalized and treated for hyponatremia and ultimately diagnosed as IAD in our hospital.
Inovasi Teknologi Media Sosial: Keberhasilan Proses Adopsi Meningkatkan Kelincahan Organisasi

This paper discussed the model of business digital with the capabilities to adopt the technological innovation of social media and to optimize the resources to improve the performance agility of business organizations. Competitiveness needs speed and innovation in responding to the exploitation of organizational improvement and benefit. This research applied a qualitative descriptive and explanatory method and referred to a number of previous studies concerning adoption, social media, and organizational agility. This research aimed to explore how far the adoption of technological innovation of social media in contributing to the formulation for the improvement of organizational performance agility and to the identification of factors ensuring the implementation success. The result showed that the adoption of technological innovation of social media affect in improving the agility of small and medium organizations. Organizations possessing agility can adapt every change quickly. The adoption success becoming the basis of digital marketing strategies depends on technology, organizations, and environment. Awareness and willingness are also determiners. Digitization makes organizations more agile in adapting the competitiveness.
Stress-Induced Gene Expression in Plants

Alterations in gene expression in response to water deficit nuclear genes expressed during seed desiccation - relationship with responses to stress gene expression in relation to salt tolerance gene expression during anoxia gene expression under low temperature stress regulation of heat shock gene expression heat shock proteins and thermotolerance ozone effects on gene expression and molecular approaches to breeding for air pollution tolerance gene expression in relation to metal toxicity and tolerance pathogen-induced gene expression.
Embryonic and Postembryonic Development of Percocypris pingi pingi （Tchang）

This study aimed to investigate the morphological characteristics of Percocypris pingi pingi( Tchang) at the embryonic and postembryonic developmental periods and to demonstrate the early developmental stage of P. pingi pingi( Tchang). According to the results,the fully mature eggs were spherical,orange-colored,sticky,2. 2 mm in diameter; the maximum outer diameter reached 3. 2- 3. 8 mm after absorbing water. When water temperature was( 18 ± 0. 5) ℃,the first cleavage occurred at 2h 28 min post-fertilization; organs began to form at 45 h 23 min post-fertilization; larvae were hatched at 126 h 28 min post-fertilization.Newly hatched larvae exhibited a total length of 10. 4 mm,with large and compressed yolk sacs. According to the morphological characteristics,the embryonic developmental process of P. pingi pingi( Tchang) could be divided into seven continuous stages,including fertilized egg stage,cleavage stage,blastula stage,early gastrula stage,neurula stage,organ formation stage and hatching stage,which were further divided into 32 phases. When water temperature ranged from 18. 8 ℃to 25. 0 ℃,the whole developmental process of P. pingi pingi( Tchang) from larvae to juveniles lasted 93 d. According to the morphological and ecological characteristics,the postembryonic developmental process of P. pingi pingi( Tchang) could be divided into three stages,including yolk-sac larva stage,late larva stage and junenile stage.
Added vegetable and fish oils to low‐fat pollen diets: effect on honey bee (Apis mellifera L.) consumption

Little is known about which commercial oils could be used as an ingredient in improving artificial feedstuffs for honey bees. To test whether the oils are palatable or whether honey bees showed a preference, they were added to a low-fat pollen known to be attractive to bees. Oils were added at 2% above the known oil content of the pollen to a level about half that of a range of melliferous plants. Of 27 different plant and fish-based oils, only linseed and coconut oil-enhanced pollen diets were consumed by bees at a significantly greater rate (P < 0.05) than pollen itself. Other oils added to pollen that had higher but not significantly different consumption rates by bees to pollen in palatability tests were (highest to lowest): evening primrose, almond, grape seed, apricot, olive, blended vegetable, orange essential oil (EO), linoleic acid, soyabean, avocado, mustard seed, cod liver, sesame and canola. Other oils found unpalatable and discarded from further testing were: gingelly, castor, peanut, rose EO, oleic acid, fish, sunflower, macadamia, rice bran, clary sage EO and lavender EO. At 2%, two of the four essential oils tested were found to be significantly unpalatable and not preferred by bees. The concentration of lavender and clary sage in a pollen diet was perhaps too high. Using alcohol via the addition of 2% rum to enhance volatiles from the pollen diet effectively increased consumption of pollen diets by honey bees. Using both palatability and preference testing methodologies to determine the attractiveness of diets to bees gave the same result. Either test will give the researcher a way of determining the food value of ingredients in diets or full diets.
Principles for Designing an AI Competition, or Why the Turing Test Fails as an Inducement Prize

If the artificial intelligence research community is to have a challenge problem as an incentive for research, as many have called for, it behooves us to learn the principles of past successful inducement prize competitions. Those principles argue against the Turing test proper as an appropriate task, despite its appropriateness as a criterion (perhaps the only one) for attributing intelligence to a machine.
Intra- and interspecific plant-soil interactions, soil legacies and priority effects during old-field succession

1. Legacy effects of plant influences on abiotic and biotic soil properties can result in priority effects that influence the structure and composition of plant communities. To better understand the role of these plant–soil interactions, here we expand the concept of plant–soil feedbacks from a within-species approach (intraspecific plant–soil feedback) to a between-species approach (interspecific plant–soil interactions). 2. In a greenhouse experiment, we tested how the early successional Jacobaea vulgaris affects its own performance and the performance of 30 co-occurring plant species via changes in abiotic and biotic soil conditions. In addition, we examined the reciprocal effect of the co-occurring species on J. vulgaris. 3. Our study had three important results. First, J. vulgaris exhibits strong negative plant–soil feedback. Secondly, there were large differences among the co-occurring species in interspecific plant–soil effects on J. vulgaris growth. Approximately, half the species reduced J. vulgaris performance, whereas the other half had no effect. Thirdly, soil conditioned by J. vulgaris had a positive or neutral effect on the growth of the co-occurring species. 4. To test the soil effects of entire plant communities, in 10 old-fields that differed in time since abandonment we recorded the identity of all plants surrounding J. vulgaris individuals. We calculated the weighted soil effect of this community on J. vulgaris and the reciprocal effect of J. vulgaris on the community. There was a positive linear relationship between time since abandonment and the weighted feedback effect of J. vulgaris on the plant community. 5. We suggest three mechanisms how the legacy of plant–soil interactions may enhance the rate of succession through priority effects: early successional plant species exert negative plant–soil feedback; co-occurring plant species cause negative interspecific plant–soil effects to the early successional species; and the early successional species have overall positive interspecific plant–soil effects on the co-occurring plant species. 6.Synthesis. The performance of an early successional species can be reduced directly by the legacy effects of intraspecific plant–soil feedback, as well as indirectly by the legacy effects of both intra- and interspecific plant–soil interactions. These intra- and interspecific plant–soil interactions can prioritize transitions of plant species in plant communities.
Users psychological profiles for leisure activity recommendation: user study

Leisure activities are essential in the individual's pursuit of well-being and higher satisfaction with life. In the last few years, the event-based social networks and event-promoting services on Face-book, Couchsurfing etc., have seen a boosting use. Their users are looking for personalised experience but they are often overwhelmed with the number of available options. To overcome this issue, recommender systems serve as users' personal guides. They exploit various techniques and types of influence (geographic, temporal, social, etc.). In this work, we investigate the impact of individuals' psychological profiles on their selection of leisure activities. We describe a comprehensive user study that we have conducted and an associated collection that could be further exploited for the recommendation of leisure activities.