Miracle-dz/UniFAR-FTUs huggingface dataset:
Herbivory, plant resistance, and climate in the tree ring record: Interactions distort climatic reconstructions

To understand climate change, dendrochronologists have used tree ring analyses to reconstruct past climates, as well as ecological processes such as herbivore population dynamics. Such reconstructions, however, have been hindered by a lack of experiments that separate the influences of confounding impacts on tree rings, such as herbivores and the interactions of multiple factors. Our long-term experiments with scale insects on resistant and susceptible pines demonstrate three major points that are important to the application of this commonly used tool. (i) Herbivory reduced tree ring growth by 25–35%. (ii) The impact on ring growth distorted climate reconstruction, resulting in the overestimation of past moisture levels by more than 2-fold. Our data suggest that, if distortion because of herbivory has been a problem in previous reconstructions, estimates of the magnitude of recent climate changes are likely to be conservative. (iii) Our studies support a detectible plant resistance × herbivore × climate interaction in the tree ring record. Because resistance and susceptibility to herbivory are known to be genetically based in many systems, the potential exists to incorporate plant genetics into the field of dendrochronology, where it may be used to screen distortions from the tree ring record.
The GAy MEn Sex StudieS: design of an online registration of sexual behaviour of men having sex with men and preliminary results (GAMESSS-study).

BACKGROUND There has been limited investigation of the sexuality and sexual dysfunction in homosexuals by the sexual medicine community. The purpose of this article is to describe the methodology of the online GAy MEn Sex StudieS (GAMESSS) on the sexual behaviour and sexual dysfunctions of Belgian men who have sex with men (MSM). AIM To describe the methodology of an online study that investigate sexual behaviour and sexual function and dysfunction in a sample of Belgian MSM. METHODS An internet-based survey on sexual behaviour and sexual dysfunctions was administered to MSM between April and December 2008. The questionnaire was a compilation of the Kinsey Scale for Sexual Orientation, the Index of Premature Ejaculation (IPE), the Erection Quality Scale (EQS), the Female Sexual Function Index (FSFI), the Brief (Male) Sexual Function Inventory (BSFI) and the Gay Men Sexual Addiction Screening Test (G-SAST). The analytic sample comprised 1,830 Belgian men aged 18 years or older, who reported having sex with men. CONCLUSION The use of an online questionnaire is a convenient way to gather information from a hidden population such as MSM. The anonymity of the participants is guaranteed. The collected data can be easily analyzed. With this online study, we aim to detect variables in sexual behaviour (SB) and sexual dysfunction (SD) that can help to improve care for MSM. Belgian MSM have a very active and varied sex life. They are rather promiscuous and do not always practice safe sex. This behaviour poses a high risk of spreading sexually transmitted diseases. Additional research in this MSM population is needed.
Root surfaces after ultrasonic instrumentation with a polishing fluid.

OBJECTIVE The recently introduced Vector system is recommended to be used in conjunction with a polishing fluid containing hydroxylapatite granules (<10 Microm). The aim of the present study was to assess morphologic alterations of the root surface after ultrasonic treatment with the polishing fluid. METHOD AND MATERIALS Cementum of 30 freshly extracted human teeth was removed to a level of 4 mm below the cementoenamel junction. Surfaces were root-planed and conditioned with citric acid to remove the smear layer. Teeth were divided into 5 groups, with group 1 as the untreated control. Group 2 was solely treated with the ultrasonic system. In group 3, the ultrasonic system was used in conjunction with the polishing fluid. Group 4 was treated with a medium-hard toothbrush and the polishing fluid. Group 5 was treated only with the toothbrush. Specimens were evaluated by both light and scanning electron microscopy. RESULTS Dentinal tubules were not covered by a smear layer in the control group, whereas in group 2, a smear layer could be observed in some specimens. In group 3 dentinal tubules were always covered by a layer consisting of granules of about 2 Microm. In group 4 all samples showed a layer containing granules of about 5 Microm, and in group 5 a thin smear layer could be observed. CONCLUSION The use of a polishing fluid containing hydroxylapatite granules during treatment of the root surface results in a granular layer covering the dentinal tubules. This layer may reduce hypersensitivity after ultrasonic subgingival scaling.
Leadership mindset: the existence or absence of leadership styles that encourages talent management and its practices and how this affects both career orientation and burnout

Orientation: Leadership is both a theoretical and practical field, existing for the sole purpose of guiding individuals and teams towards desired organizational goals. Leaders have the responsibility to capacitate their sub-ordinates to ensure effective job performance. Even though organizations have policies and a specific culture, leaders and managers must constantly evaluate their practices in order to identify and encourage factors that may positively contribute to the realization of employees’ and organizational goals. Research Purpose: The objective of this research was to examine and appraise the leadership style of professionals within the North-West Provincial Department. Research approach, design and method: A descriptive measure was utilized with a stratified random sample (N = 205). The Multifactor Leadership Questionnaire and a biographical questionnaire were administered to investigate the leadership patterns of the participants. An exploratory factor analysis was used to determine the underlying factor structure of the variables. Main findings: The results suggest that there are insignificant but positive differences amongst different leadership patterns displayed by the professional employees in the NorthWest provincial department that was the research site. Practical/managerial implications: Leadership goes a long way in ensuring efficient practices and professionals within the department have to be further developed to ensure that transformational leadership traits are firmly instilled within them to enhance the success of employees, organizational teams and the organization at large. Contribution: The findings in this study increase the body of knowledge in the field of leadership of professional employees working in a professional department. This research generates new knowledge regarding the specific leadership styles that professionals display.
Progestin Receptor Immunoreactivity Within Steroid‐Responsive Vasopressin‐Immunoreactive Cells in the Male and Female Rat Brain

Progestin receptor immunoreactivity is found in the same regions of the bed nucleus of stria terminalis (BST) and centromedial amygdala (CMA) as steroid‐responsive vasopressin immunoreactive (AVP‐ir) cells. To test whether AVP‐ir cells express progestin receptors, brains of male rats were stained immunocytochemically for arginine vasopressin as well as progestin receptors. In BST and CMA, over 95% of AVP‐ir cells contained progestin receptor immunoreactivity. In contrast, none of the AVP‐ir cells in the suprachiasmatic, supraoptic and paraventricular nuclei expressed progestin receptor immunoreactivity. To study whether progestin receptor expression in AVP‐ir cells in the BST and CMA is responsive to gonadal steroids, male and female rats were castrated and implanted with either empty capsules or capsules filled with testosterone or oestradiol, respectively. Ten days later, brains were processed for AVP and progestin receptor immunoreactivity. Although there was no effect of hormonal status on the percentage of colocalized cells, the level of progestin receptor immunoreactivity was higher in rats that received gonadal steroids than those that did not. The presence of progestin receptor immunoreactivity in steroid responsive AVP‐ir cells, and the responsiveness of this expression to gonadal hormones, is consistent with the possibility that the effects of gonadal steroids on AVP‐ir expression in the BST and CMA may be mediated at least in part by progestin receptors.
14‐Deoxyandrographolide desensitizes hepatocytes to tumour necrosis factor‐alpha‐induced apoptosis through calcium‐dependent tumour necrosis factor receptor superfamily member 1A release via the NO/cGMP pathway

Andrographis paniculata (AP) has been found to display hepatoprotective effect, although the mechanism of action of the active compounds of AP in this context still remains unclear. Here, we evaluated the hepatoprotective efficacy of 14‐deoxyandrographolide (14‐DAG), a bioactive compound of AP, particularly its role in desensitization of hepatocytes to tumour necrosis factor‐alpha (TNF‐α)‐induced signalling of apoptosis.
Decaprenyl Diphosphate Synthesis in Mycobacterium tuberculosis

ABSTRACT Z-prenyl diphosphate synthases catalyze the sequential condensation of isopentenyl diphosphate with allylic diphosphates to synthesize polyprenyl diphosphates. In mycobacteria, these are precursors of decaprenyl phosphate, a molecule which plays a central role in the biosynthesis of essential mycobacterial cell wall components, such as the mycolyl-arabinogalactan-peptidoglycan complex and lipoarabinomannan. Recently, it was demonstrated that open reading frame Rv2361c of the Mycobacterium tuberculosis H37Rv genome encodes a unique prenyl diphosphate synthase (M. C. Schulbach, P. J. Brennan, and D. C. Crick, J. Biol. Chem. 275:22876-22881, 2000). We have now purified the enzyme to near homogeneity by using an Escherichia coli expression system and have shown that the product of this enzyme is decaprenyl diphosphate. Rv2361c has an absolute requirement for divalent cations and an optimal pH range of 7.5 to 8.5, and the activity is stimulated by both detergent and dithiothreitol. The enzyme catalyzes the addition of isopentenyl diphosphate to geranyl diphosphate, neryl diphosphate, ω,E,E-farnesyl diphosphate, ω,E,Z-farnesyl diphosphate, or ω,E,E,E-geranylgeranyl diphosphate, with Km values for the allylic substrates of 490, 29, 84, 290, and 40 μM, respectively. The Km value for isopentenyl diphosphate is 89 μM. The catalytic efficiency is greatest when ω,E,Z-farnesyl diphosphate is used as the allylic acceptor, suggesting that this is the natural substrate in vivo, a conclusion that is supported by previous structural studies of decaprenyl phosphoryl mannose isolated from M. tuberculosis. This is the first report of a bacterial Z-prenyl diphosphate synthase that preferentially utilizes an allylic diphosphate primer having the α-isoprene unit in the Z configuration, indicating that Rv1086 (ω,E,Z-farnesyl diphosphate synthase) and Rv2361c act sequentially in the biosynthetic pathway that leads to the formation of decaprenyl phosphate in M. tuberculosis.
Technology and Sector Clustering in the Greek Economy

The aim of this paper is to identify the economic sectors in the Greek economy which are dynamic in terms of their technological characteristics expressing its economic perspectives. To do this, the paper uses the Clustering Analysis methodology for grouping the various sectors of economic activity in Greece. The twenty-one sectors of economic activity are thus assembled into clusters presenting similar technology characteristics and the empirical results are discussed. The technical analysis is based on Growth Accounting methodology to estimate technological change, as well as labor and capital productivity in the various sectors of the Greek economy over the period 1988-1998. The results show that the various sectors of economic activity tend to form three (3) distinct clusters experiencing similar technological and growth characteristics. Meanwhile, the technological level, as measured through annual growth in Total Factor Productivity, has remained practically unchanged. Finally, technological change accounts for about 40% of economic growth, which is slightly lower compared with the relative performance of other O.E. C.D. countries.
Career duration in the NHL: pushing and pulling on Europeans?

ABSTRACT Using a panel of National Hockey League players from 2000 through 2013, we analyse the determinants of career length in the league. In our analysis, we include both performance variables and nationality of origin to determine their importance in determining career length. We find that European-born players have shorter careers than North American-born players holding performance constant and Russian-born players have even shorter careers than other Europeans. We further find that Russian-born players have even shorter careers than other players after the 2005 lockout. These shorter careers are consistent with both exit discrimination against European and Russian players who are pushed out of the league as well as voluntary exit by European and Russian players who are pulled out of the league due to opportunities in their home countries.
Cognitive screening for dementia in primary care: a systematic review

ABSTRACT Background: Identifying dementia in primary care could minimize the impact of a late intervention; however, it shows high rates of misdiagnosis. One of the reasons seems to be the lack of knowledge of adequate cognitive screening instruments. This is a systematic review of the available instruments for the primary care context. Method: For this systematic review, articles were collected according to the following combined key terms: “cognitive screening” and “dementia” and “primary care” and “review”. Studies should be reviews focusing on cognitive screening instruments best used in primary care setting. Results: Thirteen reviews were selected. In total, it was considered 34 cognitive screening instruments. Half of the instruments can be applied in an adequate time-limit for primary care context. Memory is the most commonly assessed cognitive function (91%). Almost half of the tests are mentioned to have influence of education or cultural factors (44%). Conclusion: Tests such as 6CIT, AMT, GPCOG, Mini-Cog, MIS, MoCA, and STMS seem to be good alternatives to the use of the Mini-Mental State Examination when considering factors such as application time, sensitivity, specificity, and number of studies. However, there is a wide range of tests with different characteristics, therefore it is recommended that the professional gets some expertise in a few number of instruments in order to be able to choose which to use, or use in combination, depending on the setting and the profile of the patient.
WHY ARE ECCENTRIC EXERCISES EFFECTIVE FOR ACHILLES TENDINOPATHY?

UNLABELLED Achilles Tendinopathy is a complex problem, with the most common conservative treatment being eccentric exercises. Despite multiple studies assessing this treatment regime little is known about the mechanism of effect. This lack of understanding may be hindering therapeutic care and preventing optimal rehabilitation. Of the mechanisms proposed, most relate to tendon adaptation and fail to consider other possibilities. The current consensus is that tendon adaptation does not occur within timeframes associated with clinical improvements, therefore the clinical benefits must occur through another unidentified pathway. This clinical commentary critically reviews each of the proposed theories and highlights that muscle alterations are observed prior to onset of Achilles Tendinopathy and during the disease. Evidence shows that the observed muscle alterations change with treatment and that these adaptations have the ability to reduce tendon load and thereby improve tendon health. The purpose of this clinical commentary is to review previous theories regarding the mechanisms by which eccentric exercise might affect Achilles tendinopathy and offers a novel mechanism by which the plantarflexor muscles may shield the Achilles tendon. LEVEL OF EVIDENCE 5.
Phosphorylation of Yeast Plasma Membrane H+-ATPase by Casein Kinase I*

The plasma membrane H+-ATPase of Saccharomyces cerevisiae is subject to phosphorylation by a casein kinase I activity in vitro. We show this casein kinase I activity to result from the combined function of YCK1 and YCK2, two highly similar and plasma membrane-associated casein kinase I homologues. First, H+-ATPase phosphorylation is severely impaired in the plasma membrane of YCK-deficient yeast strains. Furthermore, the wild-type level of the phosphoprotein is restored by the addition of purified mammalian casein kinase I to the mutant membranes. We used the H+-ATPase as well as a synthetic peptide substrate that contains a phosphorylation site for casein kinase I to compare kinase activity in membranes prepared from yeast cells grown in the presence or absence of glucose. The addition of glucose results in increased H+-ATPase activity which is associated with a decline in the phosphorylation level of the enzyme. Mutations in both YCK1 and YCK2 affect this regulation, suggesting that H+-ATPase activity is modulated by glucose via a combination of a “down-regulating” casein kinase I activity and another, yet uncharacterized, “up-regulating” kinase activity. Biochemical mapping of phosphorylated H+-ATPase identifies a major phosphopeptide that contains a consensus phosphorylation site (Ser-507) for casein kinase I. Site-directed mutagenesis of this consensus sequence indicates that Glu-504 is important for glucose-induced decrease in the apparent Km for ATP.
Balancing Forest‐Regeneration Probabilities and Maintenance Costs in Dry Grasslands of High Conservation Priority

Abstract: Abandonment of agricultural land has resulted in forest regeneration in species‐rich dry grasslands across European mountain regions and threatens conservation efforts in this vegetation type. To support national conservation strategies, we used a site‐selection algorithm (MARXAN) to find optimum sets of floristic regions (reporting units) that contain grasslands of high conservation priority. We sought optimum sets that would accommodate 136 important dry‐grassland species and that would minimize forest regeneration and costs of management needed to forestall predicted forest regeneration. We did not consider other conservation elements of dry grasslands, such as animal species richness, cultural heritage, and changes due to climate change. Optimal sets that included 95–100% of the dry grassland species encompassed an average of 56–59 floristic regions (standard deviation, SD 5). This is about 15% of approximately 400 floristic regions that contain dry‐grassland sites and translates to 4800–5300 ha of dry grassland out of a total of approximately 23,000 ha for the entire study area. Projected costs to manage the grasslands in these optimum sets ranged from CHF (Swiss francs) 5.2 to 6.0 million/year. This is only 15–20% of the current total estimated cost of approximately CHF30–45 million/year required if all dry grasslands were to be protected. The grasslands of the optimal sets may be viewed as core sites in a national conservation strategy.
Differential Effects of Sucrose and Auxin on Localized Phosphate Deficiency-Induced Modulation of Different Traits of Root System Architecture in Arabidopsis1[C][W][OA]

Phosphorus, one of the essential elements for plants, is often a limiting nutrient in soils. Low phosphate (Pi) availability induces sugar-dependent systemic expression of genes and modulates the root system architecture (RSA). Here, we present the differential effects of sucrose (Suc) and auxin on the Pi deficiency responses of the primary and lateral roots of Arabidopsis (Arabidopsis thaliana). Inhibition of primary root growth and loss of meristematic activity were evident in seedlings grown under Pi deficiency with or without Suc. Although auxin supplementation also inhibited primary root growth, loss of meristematic activity was observed specifically under Pi deficiency with or without Suc. The results suggested that Suc and auxin do not influence the mechanism involved in localized Pi sensing that regulates growth of the primary root and therefore delineates it from sugar-dependent systemic Pi starvation responses. However, the interaction between Pi and Suc was evident on the development of the lateral roots and root hairs in the seedlings grown under varying levels of Pi and Suc. Although the Pi+ Suc− condition suppressed lateral root development, induction of few laterals under the Pi− Suc− condition point to increased sensitivity of the roots to auxin during Pi deprivation. This was supported by expression analyses of DR5∷uidA, root basipetal transport assay of auxin, and RSA of the pgp19 mutant exhibiting reduced auxin transport. A significant increase in the number of lateral roots under the Pi− Suc− condition in the chalcone synthase mutant (tt4-2) indicated a potential role for flavonoids in auxin-mediated Pi deficiency-induced modulation of RSA. The study thus demonstrated differential roles of Suc and auxin in the developmental responses of ontogenetically distinct root traits during Pi deprivation. In addition, lack of cross talk between local and systemic Pi sensing as revealed by the seedlings grown under either the Pi− Suc− condition or in the heterogenous Pi environment highlighted the coexistence of Suc-independent and Suc-dependent regulatory mechanisms that constitute Pi starvation responses.
Advancing geriatrics education: an efficient faculty development program for academic hospitalists increases geriatric teaching.

BACKGROUND Hospitalists care for an increasing number of older patients. As teachers, they are uniquely positioned to teach geriatric skills to residents. Faculty development programs focused on geriatrics teaching skills are often expensive and time-intensive, and may not enhance trainee learning. OBJECTIVES To evaluate a train-the-trainer (TTT) model designed to equip hospitalists with knowledge and skills to teach geriatric topics to residents in a time-constrained, resource-limited environment. DESIGN Cross-sectional survey. SETTING Academic tertiary hospital. INTERVENTION A 10-hour geriatric curriculum, the Reynolds Program for Advancing Geriatrics Education (PAGE), cotaught by geriatricians and hospitalists at preexisting noon conferences over 1 year that consisted of exportable teaching modules. MEASUREMENTS Session leaders' and faculty participants' satisfaction, hospitalist geriatrics teaching self-efficacy, residents' self-report of frequency of geriatric teaching received, and frequency of geriatric skill use. RESULTS The curriculum was highly rated by session leaders and hospitalist faculty. Hospitalists perceived improvement in geriatric teaching skills, indicating (1: "unlikely" to 5: "highly likely") that they are likely to use these teaching tools in the future (M = 4.61, standard deviation [SD] = 0.53). Residents reported both significantly more geriatrics teaching by hospitalists (P < 0.05) and a borderline significant increase in their practice of geriatric clinical skills (P = 0.05). CONCLUSIONS A time-efficient geriatric faculty development program for hospitalists suggests improvement in the amount and quality of geriatrics teaching and skill practice among faculty and residents at an academic medical center. Concise faculty development programs within preexisting faculty meetings may be a feasible, successful method to increase geriatric skill development in the hospital setting.