Prevalence of Chlamydia trachomatis in women of Al-Diwanyia city

This work included three groups of women who attended to the maternity and pediatrics hospital in the period march to July 2010, group A were healthy asymptomatic unmarried women included 168 sample, group B was 122 asymptomatic married women and group C included 123 women who were suffering from spontaneous abortion identified by gynecologist and sera samples were screened by anti-Chlamydia IgG Elisa kit . Obtained results showed that the total tested cases was 413 included 383 (92.74%) case were negative and 30 (7.26%) sample gave positive result to C. trachomatis . Most cases were recorded the highest ration during the age group 20-40 years old . Prevalence of C. trachomatis in healthy asymptomatic unmarried women was 4 (2.38 %) as shown in , while in the second group of healthy asymptomatic married women was 5 (4.09%) . The highest prevalence ratio was documented in the group of women who were suffering from spontaneous abortion ,about 21(17%) . Obtained results indicate the importance of chlamydia infections among women in which abortion and other medical complication could occur, which needs an early testing for the infection . ةصلاخلا زوــمت ىـلإ راذآ ةرـتفلا نـم ةــیناویدلا ةـنیدم يـف لاـفطلأاو ةیئاسـنلا ىفشـتسم نـعجا ر يتاوـللا ءاسـنلا نـم عیماــجم ةـثلاث لـمعلا اذـه نمضـت ٢٠١٠ . تنمضت يتلاو تاجوزتم ریغلا ءاحصلأا ءاسنلا نم تناك ىلولأا ةعومجملا ١٦٨ تلمش ةیناثلا ةعومجملا و ةنیع ١٢٢ نم ةنیع ســنلا ضا رــعأ نودــب تاــجوزتملا ءا ، تنمضــت ةــثلاثلا ةــعومجملا ١٢٣ تصــخش طاقــسإ نــم نیناــع يتاوــللا ءاســنلا نــم ةــنیع لاصــملأا تاــنیع عون ایدیملاكلا دادضلأ ا زیللاا صحفب IgG ، ناـك رـبتخا يذـلا يـلكلا تلااحلا ددع ٤١٣ تنمضـت ةـنیع ٣٨٣ ) ٩٢. ٧٤ (% و ةبلاـس تـناك ٣٠ ) ٧. ٢٦ (% ةبجوم ةجیتن تطعأ ةنیع ایدیملاكلا ببسب . نیـب اـم ةـیرمعلا تاـئفلا يـف ةباصلإا تزكرت ٢٠ ٤٠ ةنـس . جئاـتنلا تـنیب كلذـك تاــجوزتملا رــیغ ءاحــصلأا ءاســنلا نیــب ایدــیملاكلا راشــتنا نأ ىــلإ ةلصــحتسملا ٥ ) ٢. ٣٨ (% ، ةــجیتنلا تــناك ةــیناثلا ةــعومجملا يــف اــمنیب ةبجوم ٥ ) ٤. ٠٩ (% ةنیع . ىلعلأا راشتنلاا ةبسن تلجسو يلاوـح تـناك ثـیح طاقـسلإا نـم نیناع يتاوللا ءاسنلا تلمش يتلا ةعومجملا دنع ٢١ ) ١٧ . (% ىلإ جاتحت يتلا و ةیبناج ضا رعأو طاقسإ نم نیناع يتاوللا ءاسنلا نیب ایدیملاكلاب ةباصلإا ةیمهأ ىلإ ةلجسملا جئاتنلا تراشأو نه نم ةصاخو ءاسنلا ىلع ا رطخ هفصوب ةباصلإا نع ركبملا فشكلا لمحلا رمعب . Introduction: Chlamydia trachomatis is an obligate intracellular gram negative bacteria that infect the epithelial cells of the lower genital tract in both males and females. These organisms are members of the family Chlamydiaceae, an unusual group of obligate intracellular bacteria (Brooks et. al.,2001) . The members of this family are considered to be Gram negative, but are difficult to stain with the Gram. They have metabolic and structural differences from most bacteria, including a dependence on adenosine triphosphate(ATP)and guanosine triphosphate (GTP) from the host(Mims et.al, 1995). Infection with C. trachomatis can led to severe complications of the reproductive tract and adverse pregnancy outcomes. The common clinical manifestations of this infection include cervicitis, pelvic inflammatory disease and tubal factor infertility (Singh el. al., 2010). In pregnant women, Chlamydia infection has been associated with an increased Journal of Babylon University/Pure and Applied Sciences/ No.(4)/ Vol.(21): 2013 ١٢٣٣ risk of ectopic pregnancy, preterm delivery, spontaneous abortion, low birth weight, premature rupture of membranes, perinatal mortality and postpartum endometritis (Mardh, 2002). Reports of spontaneous abortion in women caused by Chlamydia date back to 1950s. Chlamydia is the most common reported noticeable disease in the USA and the leading cause of bacterial STI in unindustrialized countries (Popischil et. al.,2002). It is estimated that as many 50% of infected men and 70% of infected women unknowingly harbour C. trachomatis (Gerbase et. al.,1998), which can persist for up to 3 months (Rahm et. al.,1986) and probably longer. The asymptomatic nature of C. trachomatis infection may facilitate its spread in the at risk population and promote a reservoir of infection (Ripa,1990). Abortion represent an important problem in Iraq, several epidemiological studies had been carried in an attempt to determine the nature and causes of such cases. Abortion in human cases was caused mainly by Toxoplasma gonidii and Cytomegalovirus. This study was aimed to investigate the focusing on the prevalence of C. trachomatis in both healthy asymptomatic and aborted women of AlQadisyia city/Iraq. Methods: This work included three groups of Iraqi women who were attended to the central bank of blood testing and the Maternity and pediatrics teaching hospital of AlDiwanyia city , group A were healthy asymptomatic unmarried women included 168 sample, group B was 122 asymptomatic married women and group C included 123 women who were suffering from spontaneous abortion identified by gynecologist, sera samples were collected in clean containers and were tested for anti-Chlamydia trachomatis IgG Elisa kit (abcam.USA). Procedure was carried as recommended by manufacture, Elisa for Detection of Chlamydia trachomatis by direct immunoenzymatic method of the sandwich type(Baron et. al.,1994). Statistical analysis included estimation of LSD on probability level p<0.01 by using SPSS soft were version 10.0, graphs were done by using Excel (Niazi, 2000). Results: Obtained results showed that the total tested cases was 413 included 383 (92.74%) case were negative and 30 (7.26%) sample gave positive result to C. trachomatis (fig-1). Most cases were recorded the highest ration during the age group 20-40 years old (fig-2). Prevalence of C. trachomatis in healthy asymptomatic unmarried women was 4 (2.38 %) as shown in (fig-3), while in the second group of healthy asymptomatic married women was 5 (4.10%) (fig-4). The highest prevalence ratio was documented in the group of women who were suffering from spontaneous abortion ,about 21(17.07%) (fig-5).