Miracle-dz/UniFAR-FTUs huggingface dataset:
Relevance of Blood–Brain Barrier Disruption After Endovascular Treatment of Ischemic Stroke: Dual-Energy Computed Tomographic Study

Background and Purpose— Computed tomographic (CT) high attenuation (HA) areas after endovascular therapy for acute ischemic stroke are a common finding indicative of blood–brain barrier disruption. Dual-energy CT allows an accurate differentiation between HA areas related to contrast staining (CS) or to brain hemorrhage (BH). We sought to evaluate the prognostic significance of the presence of CS and BH after endovascular therapy. Methods— A prospective cohort of 132 patients treated with endovascular therapy was analyzed. According to dual-energy CT findings, patients were classified into 3 groups: no HA areas (n=53), CS (n=32), and BH (n=47). The rate of new hemorrhagic transformations was recorded at follow-up neuroimaging. Clinical outcome was evaluated at 90 days with the modified Rankin Scale (poor outcome, 3–6). Results— Poor outcome was associated with the presence of CS (odds ratio [OR], 11.3; 95% confidence interval, 3.34–38.95) and BH (OR, 10.4; 95% confidence interval, 3.42–31.68). The rate of poor outcome despite complete recanalization was also significantly higher in CS (OR, 9.7; 95% confidence interval, 2.55–37.18) and BH (OR, 15.1; 95% confidence interval, 3.85–59.35) groups, compared with the no-HA group. Patients with CS disclosed a higher incidence of delayed hemorrhagic transformation at follow-up (OR, 4.5; 95% confidence interval, 1.22–16.37) compared with no-HA patients. Conclusions— Blood–brain barrier disruption, defined as CS and BH on dual-energy CT, was associated with poor clinical outcomes in patients with stroke treated with endovascular therapies. Moreover, isolated CS was associated with delayed hemorrhagic transformation. These results support the clinical relevance of blood–brain barrier disruption in acute stroke.
The Social Benefits of Balancing Creativity and Imitation: Evidence from an Agent-based Model

Although creativity is encouraged in the abstract it is often discouraged in educational and workplace settings. Using an agent-based model of cultural evolution, we investigated the idea that tempering the novelty-generating effects of creativity with the novelty-preserving effects of imitation is beneficial for society. In Experiment One we systematically introduced individual differences in creativity, and observed a trade-off between the ratio of creators to imitators, and how creative the creators were. Excess creativity was detrimental because creators invested in unproven ideas at the expense of propagating proven ones. Experiment Two tested the hypothesis that society as a whole benefits if individuals adjust how creative they are in accordance with their creative success. When effective creators created more, and ineffective creators created less (social regulation), the agents segregated into creators and imitators, and the mean fitness of outputs was temporarily higher. We hypothesized that the temporary nature of the effect was due to a ceiling on output fitness. In Experiment Three we made the space of possible outputs open-ended by giving agents the capacity to chain simple outputs into arbitrarily complex ones such that fitter outputs were always possible. With the capacity for chained outputs, the effect of social regulation could indeed be maintained indefinitely. The results are discussed in light of empirical data.
On distributed probabilistic algorithms for computer graphics

When attempting to solve a multi-variate optimisation problem, it is often a wise strategy to sacrifice a locally-optimal solution in the hope of finding a better global solution. Algorithms that solve optimisation problems in this manner are sometimes called hill climbing algorithms . Recently, several new hillclimbing approaches have been proposed and have gained in popularity for solving special classes of optimisation problems . These approaches are probabilistic in nature. We introduce one particular approach called simulated annealing, and show how a distributed version of it can be used to solve a basic problem in computer graphics and image processing: colour q uantisation.
Supporting Community-Based Prevention and Health Promotion Initiatives: Developing Effective Technical Assistance Systems

As research evidence for the effectiveness of community-based prevention has mounted, so has recognition of the gap between research and community practice. As a result, state and local governments are taking a more active role in building the capacity of community-based organizations to deliver evidence-based prevention interventions. Innovations are taking place in the establishment of technical assistance or support systems to influence the prevention and health education activities of community-based organizations. Several challenges for technical assistance systems are described: (1) setting prevention priorities and allocating limited technical assistance resources, (2) balancing capacity-building versus program dissemination efforts, (3) collaborating across categorical problem areas, (4) designing technical assistance initiatives with enough “dose strength” to have an effect, (5) balancing fidelity versus adaptation in program implementation, (6) building organizational cultures that support innovation, and (7) building local evaluative capacity versus generalizable evaluation findings.
Anger Recognition in Spoken Dialog Using Linguistic and Para-Linguistic Information

This paper proposes a method to recognize anger-dialog based on linguistic and para-linguistic information in speech. Anger is classiﬁed into two types; HotAnger (agitated) and ColdAnger (calm). Conventional prosody-features based on para-linguistic can reliably recognize the former but not the latter. To recognize anger more robustly, we apply other para-linguistic cues named dialog-features which are seen in conversational interactive situations between two speakers such as turn-taking and back-channel feedback. We also utilize linguistic-features which represent conversational emotional salience. They are acquired by Pearson’s chi-square test by comparing the automatically-transcribed texts between angry and neutral dialogs. Experiments show that the proposed feature combination improves the F-measure of ColdAnger and HotAnger by 26.9 points and 16.1 points against a baseline that uses only prosody.
Methodological issues in the evaluation of Internet-based interventions for problem drinking.

INTRODUCTION AND AIMS In recent years, there has been an increase in the number of Internet-based interventions (IBI) for alcohol problems and other addictive behaviours. However, it is risky to assume interventions that have been found to work in face-to-face modalities can be translated into IBI that are equally effective. DESIGN AND METHODS Using selected examples from the published works, this paper will identify some of the special considerations that are relevant to the evaluation of IBI. In addition, methodological issues found in the ongoing development and evaluation of the Check Your Drinking screener (http://www.CheckYourDrinking.net), an IBI for problem drinkers, will be discussed. RESULTS There have been several randomised control trials with promising results. A primary limitation of much of the research conducted to date is concerns regarding the generalisability of the findings. DISCUSSION AND CONCLUSIONS. Caution should be taken in assuming that the IBI, which have been found to work in tightly controlled efficacy trials, will display similar levels of effectiveness when used in 'naturalistic' settings (i.e. not face-to-face in a research environment). Positive results from studies using a variety of different research designs will advance the potential for IBI, as a new means of helping problem drinkers reduce their alcohol consumption. Because of their accessibility and anonymity, IBI could facilitate a broad provision of treatment services at a population level.
Prolonged corticosterone treatment of adult rats inhibits the proliferation of oligodendrocyte progenitors present throughout white and gray matter regions of the brain

It is well established that glucocorticoids inhibit the proliferation of progenitor cells that occurs in the hippocampal dentate gyrus of adult mammals. Active cell proliferation also occurs in the subventricular zone (SVZ) of the lateral ventricle and, to a lesser extent, throughout white and gray matter regions of the adult brain. The aim of the present study was to determine whether extrahippocampal cell proliferation is also affected by glucocorticoids. The cell proliferation marker bromodeoxyuridine (BrdU) was administered to control rats, to adrenalectomized rats, and to rats treated with a daily injection of corticosterone (10 mg/kg) for a period of 15 days. In control and adrenalectomized rats, high to low numerical densities of BrdU‐labeled nuclei were detected within the different forebrain regions examined. In rats treated with corticosterone, a dramatic decrease of cell proliferation was detected in the dentate gyrus, but also throughout all white and gray matter regions examined, except for the SVZ of the lateral ventricle. Double‐labeling experiments indicated that throughout the different white and gray forebrain regions examined, except for the SVZ, BrdU‐labeled nuclei were essentially associated with cells immunostained for the marker of oligodendrocyte progenitors NG2. These data indicate that glucocorticoids inhibit the proliferation of oligodendrocyte precursors located throughout the white and gray matter regions of the adult rat brain. Since the proliferation of oligodendrocyte precursors plays a major role in the processes of remyelination, these data raise the question of possible detrimental effects of therapeutic treatments of CNS trauma based on the administration of glucocorticoids. GLIA 31:219–231, 2000. © 2000 Wiley‐Liss, Inc.
Corporate Social Responsibility and Firms’ Performance: A Stratigraphical Analysis

Over the last two decades in OECD countries an increasing number of firms are obtaining certification as Socially Responsible (CSR is the acronym for Corporate Social Responsibility). Several studies (including Preston and O’Bannon, 1997; Waddock and Graves, 1997; McWilliams and Sieger, 2001; Ullman, 1985) have sought to test whether there is a relation between Social Responsibility certification and the firms’ performance. Our work builds a CSR index that intersects two of the three main international indices (Domini 400 Social Index, Dow Jones Sustainability World Index, FTSE4Good Index), in order to overcome some problems related to the multiplicity of CSR definitions and certifications. By using this database, our work carries out a stratigraphical analysis in order to verify whether some variables are statistically different in the CSR group with respect to the benchmark case (non-CSR). The main results show that there are several interesting differences in some economic indicators between CSR and non-CSR firms and between USA and EU, and among different industrial sectors.
Simultaneous analysis of endogenous neurotransmitters and neuropeptides in brain tissue using capillary electrophoresis — microelectrospray‐tandem mass spectrometry

Capillary electrophoresis was combined with highly sensitive microelectrospray‐tandem mass spectrometry to simultaneously detect classical small molecule neurotransmitters as well as neuropeptides from discrete regions of the marmoset brain. A mixture of four classical neurotransmitters (glutamate, γ‐aminobutyric acid, acetylcholine, dopamine) and four neuropeptides (neurotensin, methionine‐enkephalin, leucine‐enkephalin and substance P 1—7) was studied to optimize the capillary electrophoresis conditions for separation, injection volume, and analysis time. γ‐Aminopropyltriethoxysilane‐coated capillaries and acetic acid electrolytes were used to avoid interactions between the sample and the capillary surface and to obtain a high anodic electroosmotic flow, which resulted in a short analysis time. Detection was performed using tandem mass spectrometry in the selected reaction monitoring mode using a triple quadrupole mass spectrometer. Samples were dissolved in ammonium acetate to achieve a transient‐isotachophoretic concentration step at the beginning of the separation and to make it possible to inject larger sample volumes, up to 140 nL. Small amounts of tissue from specific regions of the marmoset monkey brain were pretreated using solid‐phase extraction as a clean‐up and concentrating step. In the striatum we could detect endogenous glutamate, γ‐aminobutyric acid (GABA), acetylcholine and dopamine, as well as the neuropeptides methionine‐enkephalin and substance P 1—7 in the same analysis, using only 58 mm3 of brain tissue.
P2Y2 Receptor Regulates VCAM-1 Membrane and Soluble Forms and Eosinophil Accumulation during Lung Inflammation

ATP has been defined as a key mediator of asthma. In this study, we evaluated lung inflammation in mice deficient for the P2Y2 purinergic receptor. We observed that eosinophil accumulation, a distinctive feature of lung allergic inflammation, was defective in OVA-treated P2Y2-deficient mice compared with OVA-treated wild type animals. Interestingly, the upregulation of VCAM-1 was lower on lung endothelial cells of OVA-treated P2Y2−/− mice compared with OVA-treated wild type animals. Adhesion assays demonstrated that the action of UTP on leukocyte adhesion through the regulation of endothelial VCAM-1 was abolished in P2Y2-deficient lung endothelial cells. Additionally, the level of soluble VCAM-1, reported as an inducer of eosinophil chemotaxis, was strongly reduced in the bronchoalveolar lavage fluid (BALF) of P2Y2-deficient mice. In contrast, we observed comparable infiltration of macrophages and neutrophils in the BALF of LPS-aerosolized P2Y2+/+ and P2Y2−/− mice. This difference could be related to the much lower level of ATP in the BALF of LPS-treated mice compared with OVA-treated mice. Our data define P2Y2 as a regulator of membrane and soluble forms of VCAM-1 and eosinophil accumulation during lung inflammation.
Models and algorithms to study the common evolutionary history of hosts and symbionts.

In this Ph.D. work, we proposed models and algorithms to study the common evolutionary history of hosts and symbionts. The first goal was to analyse the robustness of the methods of phylogenetic tree reconciliations, which are a common way of performing such study. This involves mapping one tree, most often the symbiont’s, to the other using a so-called event-based model. The events considered in general are cospeciation, duplication, host switch, and loss. The host and the symbiont phylogenies are usually considered as given and without any errors. The objective here was to understand the strengths and weaknesses of the parsimonious model used in such mappings of one tree to another, and how the final results may be influenced when small errors are present, or are introduced in the input datasets. This may correspond either to a wrong choice of present-day symbiont-host associations in the case where multiple ones exist, or to small errors related to a wrong rooting of the symbiont tree. Our results show that the choice of leaf associations and of root placement may have a strong impact on the variability of the reconciliation output. We also noticed that the host switch event has an important role in particular for the rooting problem. The second goal of this Ph.D. was to introduce some events that are little or not formally considered in the literature. One of them is the spread, which corresponds to the invasion of different hosts by a same symbiont. In this case, as when spreads are not considered, the optimal reconciliations obtained will depend on the choice made for the costs of the events. The need to develop statistical methods to assign the most appropriate ones therefore remains of actuality. Two types of spread are introduced: vertical and horizontal. The first case corresponds to what could be called also a freeze in the sense that the evolution of the symbiont “freezes” while the symbiont continues to be associated with a host and with the new species that descend from this host. The second includes both an invasion, of the symbiont which remains with the initial host but at the same time gets associated with (“invades”) another one incomparable with the first, and a freeze, actually a double freeze as the evolution of the symbiont “freezes” in relation to the evolution of the host to which it was initially associated and in relation to the evolution of the second one it “invaded”. Our results show that the introduction of these events makes the model more realistic, but also that it is now possible to directly use datasets with a symbiont that is associated with more than one host at the same time, which was not feasible before.
Periplaneta americana Extracts Accelerate Liver Regeneration via a Complex Network of Pathways

Successful recovery from hepatectomy is partially contingent upon the rate of residual liver regeneration. The traditional Chinese medicines known as Periplaneta americana extracts (PAEs) positively influence wound healing by promoting tissue repair. However, the effect of PAEs on liver regeneration is unknown. We used a mouse liver regeneration model after 70% partial hepatectomy (PH) and a hepatocyte culture to determine whether PAEs can promote liver regeneration as effectively as skin regeneration and establish their modes of action. L02 cells were divided into serum-starved control (NC) and three PAEs (serum starvation + 0.1 mg/ml, 0.5 mg/ml, or 1 mg/ml PAEs) groups. L02 cell proliferation was assessed at 24 h, 48 h, and 72 h by CCK-8 assay. Forty male C57 mice were randomly divided into control (NC), normal saline (NS), PAEs400 (400 mg/kg/d), and PAEs800 (800 mg/kg/d) groups (n = 10 per group). The NS and both PAEs groups were administered normal saline and PAEs, respectively, by gavage for 10 days. Two hours after the tenth gavage, the NS and both PAEs groups were subjected to 70% PH and the residual liver was harvested after 48 h. The hepatic regeneration rate was evaluated and hepatocyte proliferation was estimated by immunohistochemical (IHC) staining for Ki-67. Twelve DEG libraries (three samples per group) were prepared and sequencing was performed in an Illumina HiSeq 2000 (Mus_musculus) at the Beijing Genomics Institute. The genes expressed in the liver tissues and their expression profiles were analyzed by bioinformatics. KEGG was used to annotate, enrich, and analyze the pathways. PAEs promoted hepatocyte proliferation in vitro and in vivo and accelerated mouse liver regeneration after 70% PH. The screening criteria were fold change (FC) ≥ 2 and q-value < 0.001. We identified 1,092 known DEGs in PAEs400 and PAEs800. Of these, 153 were categorized in cellular processes. The KEGG analysis revealed that the aforementioned DEGs participated in several signaling pathways closely associated with cell proliferation including PI3K-Akt, MAPK, Apelin, Wnt, FoxO, mTOR, Ras, VEGF, ErbB, Hippo, and AMPK. It was concluded that PAEs can effectively improve liver regeneration via the synergistic activation of different signaling pathways.
Repair of Iatrogenic Temporal Lobe Encephalocele after Canal Wall Down Mastoidectomy in the Presence of Active Cholesteatoma

Objective: Although mastoid and middle ear obliteration provides the ultimate repair of an encephalocele, retained squamous epithelium may result in the occult recurrence of cholesteatoma. For most patients, a preferable technique is to perform a canal-wall-up mastoidectomy with middle fossa craniotomy. However, temporal lobe encephaloceles are occasionally found in patients with canal-wall-down cavities along with active cholesteatoma. We sought to describe our management strategy for this dilemma. Study Design: Retrospective review. Setting: Tertiary referral center. Patients: We reviewed all patients with encephaloceles treated by the primary surgeon. Patients without active cholesteatoma and a canal-wall-down cavity were excluded. Intervention: Surgical management of the encephalocele and cholesteatoma. Main Outcome Measure: Successful repair and a noninfected ear. Results: Three patients met the inclusion criteria. All had previous canal-wall-down surgery for cholesteatoma by outside surgeons and presented with chronic otorrhea, large tegmen defects, and brain herniation into the mastoid cavity. All had incomplete removal of their posterior canal wall. Our management strategy involved completing the canal-wall-down mastoidectomy and repairing the temporal floor defect using a three-layer closure via a middle fossa craniotomy. This included suture repair of the dural defect with or without a graft, a temporalis muscle rotation flap, and a split-calvarial bone graft. All patients recovered from their surgery without evidence of further cerebrospinal fluid leak, encephalocele, or cholesteatoma with a minimum follow-up time of 6 months. Conclusions: A temporal lobe encephalocele can be safely repaired while maintaining a mastoid bowl. This may be the safest treatment option for patients with active cholesteatoma.
Methods to improve gene signal: Application to cDNA microarrays

Microarrays are high throughput biological assays that allow the screening of thousands of genes for their expression. The main idea behind microarrays is to compute for each gene a unique signal that is directly proportional to the quantity of mRNA that was hybridized on the chip. A large number of steps and errors associated with each step make the generated expression signal noisy. As a result, microarray data need to be carefully preprocessed before their analysis can be assumed to lead to reliable and biologically relevant conclusions. This thesis focuses on developing methods for improving gene signal and further utilizing this improved signal for higher level analysis. To achieve this, first, approaches for designing microarray experiments using various optimality criteria, considering both biological and technical replicates, are described. A carefully designed experiment leads to signal with low noise, as the effect of unwanted variations is minimized and the precision of the estimates of the parameters of interest are maximized. Second, a system for improving the gene signal by using three scans at varying scanner sensitivities is developed. A novel Bayesian latent intensity model is then applied on these three sets of expression values, corresponding to the three scans, to estimate the suitably calibrated true signal of genes. Third, a novel image segmentation approach that segregates the fluorescent signal from the undesired noise is developed using an additional dye, SYBR green RNA II. This technique helped in identifying signal only with respect to the hybridized DNA, and signal corresponding to dust, scratch, spilling of dye, and other noises, are avoided. Fourth, an integrated statistical model is developed, where signal correction, systematic array effects, dye effects, and differential expression, are modelled jointly as opposed to a sequential application of several methods of analysis. The methods described in here have been tested only for cDNA microarrays, but can also, with some modifications, be applied to other high-throughput technologies.