Miracle-dz/UniFAR-FTUs huggingface dataset:
Mitochondrial haplogroups in patients with rheumatoid arthritis: No association with disease and disease manifestations

Objective To describe the distribution of specific mitochondrial DNA (mtDNA) haplogroups (hgs) in a cohort of patients with rheumatoid arthritis (RA). Methods Two-hundred nineteen consecutive patients with RA had mtDNA isolated from their blood, sequenced and haplotyped. Patients were diagnosed according to the American College of Rheumatology (ACR)/European league against Rheumatism (EULAR) criteria. Demographic and clinical data were retrieved from the Danish nationwide database (DANBIO). Logistic regression analyses were performed to test for associations. Results One-hundred eighty-four patients were eligible for analysis. Haplogroup frequencies were: H (n = 88; 47.8%), U (n = 37; 20.1%), T (n = 22; 12.0%), J (n = 16; 8.7%), K (n = 11; 5.9%), HV (n = 6; 3.3%) and V (n = 4; 2.2%). The distribution of individual hgs was identical to the background population. Radiographic erosions were significantly associated with hg clusters JT (OR = 2.37, 95% confidence interval (CI): 1.07–5.53, p = 0.038). Significantly fewer patients from hg cluster JT received biological treatment (OR = 0.17, 95% CI: 0.03–0.87, p = 0.038). Albeit, none of these associations were significant when corrected for multiple tests. Conclusion There was no significant association between mtDNA hgs and presence of RA or disease manifestations. There was an, albeit insignificant, overrepresentation of patients with hg JT among patients with erosive disease; however, slightly fewer patients in the JT group were treated with biological drugs.
Involvement of Reactive Oxygen Species in Cytokine and Growth Factor Induction of c-fos Expression in Chondrocytes(*)

The cytokine tumor necrosis factor α (TNFα) and the growth factor basic fibroblast growth factor (bFGF) are known to induce early response genes such as c-fos and c-jun in various cell types. Activation of AP-1, a heterodimeric complex of Fos and Jun proteins, is required for matrix metalloproteinase production and cell proliferation. However, the signaling pathways by which these two factors influence the expression and activities of AP-1 remain currently poorly characterized. Several studies have shown that cytokines induce reactive oxygen species (ROS) production, but growth factor induction of ROS has not been reported. In the present study we demonstrate that both TNFα and bFGF induce ROS production, and that this is a common signaling event involved in the stimulation of c-fos gene expression in chondrocytes. To our knowledge, this is the first report directly demonstrating ROS production upon stimulation with a growth factor. TNFα and bFGF induction of ROS production is mediated through flavonoid-containing enzymes such as NADPH oxidase. Moreover, the ROS nitric oxide is not responsible for the induction of c-fos expression by TNFα and bFGF. In addition, the inhibitory effects of antioxidants on c-fos expression may account for their protective roles against proliferative and inflammatory diseases such as cancer, cardiovascular diseases, and arthritis.
Local development through rural entrepreneurship, from the Triple Helix perspective

PurposeBy using the Triple Helix model, the purpose of this paper is to uncover the perceptions of nascent entrepreneurs about a university–industry–government collaboration program, in particular about the role of each agent to foster rural entrepreneurship; the value and effect of this collaboration; and their own contributions to local development.Design/methodology/approachA qualitative case study method is adopted, involving semi-structured interviews with entrepreneurs and secondary data. The text is analyzed using qualitative content analysis.FindingsThe interaction of the Triple Helix partners is perceived as valuable both at the personal and the business levels. One of the most salient results is the value ascribed to the knowledge-rich environment created. Entrepreneurs are aware of their contribution to local development, identifying economic, social and cultural effects.Practical implicationsThe research strengthens the importance of the joint efforts of the Triple Helix partners by uncovering a number of outputs from their collaboration, which affect both the entrepreneurs and local development through entrepreneurship.Originality/valuePrevious studies assume that the Triple Helix fosters technological innovation that favors regional development, mainly by adopting a macro-level perspective. This study makes a contribution by furthering the knowledge on the micro-level dynamics of the Triple Helix, through the view of low tech, rural entrepreneurs, considering their context.
A retrospective analysis of addressing higher education students socio cultural differences in the Ethiopian educational systems and the prospect of multicultural approach

---The main purpose of this study was to examine trends of treating within-country higher education students‘ socio-cultural differences in the Ethiopian education systems retrospectively and find evidence based explanation with regard to the level of effectiveness of the current multicultural approach for managing student diversity. The study used qualitative data collected from ranges of policystrategy documents and documented higher education student intergroup scenarios in the country today. The findings showed that during the Imperial regime assimilation was practiced where as during the Dergue era integration strategy was employed to bring about social cohesion in the institutions. These approaches did not bring about the envisaged peace in the country that the present EPRDF government which had seized government power and which had emerged from the student struggle itself installed a multicultural policy. Nevertheless, ethnic, linguistic and religious based mistrust and hostilities have been prevailing at universities since the implementation of the Policy. The findings suggested that ethnic, linguistic and religious diversity variables are equally vital and that the multicultural approach hardly resolved the multifaceted student flashpoints. The study suggested that the treatment of sociocultural diversity in the present educational system seems to be at a crossroad. It was recommended that in order to mitigate the prevailing identity based mistrust and hostility of students, a transformational diversity management approach that goes beyond the ̳unity within diversity‘ in which both differences and commonalities are equally embraced and celebrated should be developed that people would develop the attitude of ―I am, because we are‖ .
Student Stakeholders Impact Redesign for Management Practicum

Healthcare educators are continually challenged to respond to societal needs as they engage in the dynamic and ongoing process of curriculum redesign.The expectations of a group of stakeholders in the process, our students, were obtained through a survey process over time and led to change in the management and leadership practicum.The authors describe the survey process and its outcomes.
Effects of the new fast forward rotating five-shift roster at a Dutch steel company

This article reports a field study of a shift roster change in a large steel producer. The changes in the roster are threefold: (1) from backward rotating to forward rotating; (2) from rather slow (three) to fast rotating (two consecutive shifts); (3) the number of days off after the night shifts was changed from two to three. Company data cover 1 year before and 1 year after the implementation of the new roster and involve all employees in the five-shift system (4600 workers) and all daytime workers (1450 workers) in technical and maintenance jobs as a control group. The study reports a decrease in absence figures (particularly on midterm sickness absence) of in total 0.6%. Furthermore, improvements in health indicators are presented, such as fatigue, musculoskeletal complaints, relationship work and health and workload in the year after implementation of the new roster. These positive effects are stronger for older workers (50 + years old). The results were significantly more positive for the shift workers compared with the control group. Statement of Relevance:This article reports an evaluation study of the largest continuous roster change in the Netherlands for decades: 4600 employees at Corus changed from a slowly backward rotating schedule to a fast forward rotating schedule. The effects are mainly positive (0.6% less absence in the first year; less fatigue). A positive association with age was also found: older workers benefit more.
A Trichomonas vaginalis 120 kDa protein with identity to hydrogenosome pyruvate:ferredoxin oxidoreductase is a surface adhesin induced by iron

Trichomonas vaginalis, a human sexually transmitted protozoan, relies on adherence to the vaginal epithelium for colonization and maintenance of infection in the host. Thus, adherence molecules play a fundamental role in the trichomonal infection. Here, we show the identification and characterization of a 120 kDa surface glycoprotein (AP120) induced by iron, which participates in cytoadherence. AP120 is synthesized by the parasite when grown in 250 µM iron medium. Antibodies to AP120 and the electro‐eluted AP120 inhibited parasite adherence in a concentration‐dependent manner, demonstrating its participation in cytoadherence. In addition, a protein of 130 kDa was detected on the surface of HeLa cells as the putative receptor for AP120. By peptide matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS), the AP120 adhesin showed homology with a hydrogenosomal enzyme, the pyruvate:ferredoxin oxidoreductase (PFO) encoded by the pfoa gene. This homology was confirmed by immunoblot and indirect immunofluorescence assays with an antibody to the carboxy‐terminus region of the Entamoeba histolytica PFO. Reverse transcription polymerase chain reaction (RT‐PCR) assays showed that a pfoa‐like gene was better transcribed in trichomonads grown in iron‐rich medium. In conclusion, the homology of AP120 to PFO suggests that this novel adhesin induced by iron could be an example of moonlighting protein in T. vaginalis.
Sampling of cache congruence classes

Techniques for sampling of cache congruence classes are considered. A sampling policy that selects on the basis of where a reference hits the cache (physically) instead of when a reference arrives is examined. The notion of weighted misses is used to provide some meaningful interpretations of the miss ratios associated with workload partitioning. <<ETX>>
Lumen pressure within obliquely insonated absorbent solid cylindrical shells with application to Doppler flow phantoms

Flow phantoms used in medical ultrasound usually employ a plastic tube as a blood vessel mimic. These tubes often have acoustic properties differing significantly from the tissue and blood-mimicking media, which results in distortion of the acoustic pressure field within the tubes and, hence, of the Doppler flow spectra. Previous analyses of this problem have used some form of the infinite plate transmission coefficient, although at least one ray-based analysis has considered a cylindrical interface but with zero wall thickness. In this paper, we compare these approximate pressure fields with the exact solution for oblique incidence on a viscoelastic cylindrical shell at 5 MHz to find for which materials the plate approximation is valid. The shell has water both inside and outside, but it can be modified to use a different fluid inside and also to include absorption in either fluid. We find the plate approximation is reasonable for soft tubes such as the copolymer Cflex (Cole-Palmer, Niles, IL) but much less so for hard tubes such as polymethylmethacrylate (PMMA).
Leptin Regulation of Neuroendocrine Systems

The discovery of leptin has enhanced understanding of the interrelationship between adipose energy stores and neuronal circuits in the brain involved in energy balance and regulation of the neuroendocrine axis. Leptin levels are dependent on the status of fat stores as well as changes in energy balance as a result of fasting and overfeeding. Although leptin was initially thought to serve mainly as an anti-satiety hormone, recent studies have shown that it mediates the adaptation to fasting. Furthermore, leptin has been implicated in the regulation of the reproductive, thyroid, growth hormone, and adrenal axes, independent of its role in energy balance. Although it is widely known that leptin acts on hypothalamic neuronal targets to regulate energy balance and neuroendocrine function, the specific neuronal populations mediating leptin action on feeding behavior and autonomic and neuroendocrine function are not well understood. In this review, we have discussed how leptin engages arcuate hypothalamic neurons expressing putative orexigenic peptides, e.g., neuropeptide Y and agouti-regulated peptide, and anorexigenic peptides, e.g., pro-opiomelanocortin (precursor of alpha-melanocyte-stimulating hormone) and cocaine- and amphetamine-regulated transcript. We show that leptin's effects on energy balance and the neuroendocrine axis are mediated by projections to other hypothalamic nuclei, e.g., paraventricular, lateral, and perifornical areas, as well as other sites in the brainstem, spinal cord, and cortical and subcortical regions.
Availability and Price of Food Products with and without Trans Fatty Acids in Food Stores around Elementary Schools in Low- and Medium-Income Neighborhoods

The purpose of this exploratory, descriptive, and cross-sectional study was to investigate the relationship between the price and availability of food products with and without trans fatty acids in food stores near elementary schools located in low- and medium-income neighborhoods of a Brazilian city. The supply of products containing trans fatty acids was higher in both regions, and these products were also cheaper. It is noteworthy that this availability may influence food choices and, consequently, the health status of children and adolescents, since this population is more likely to buy less-healthy foods when these are more available, accessible, and financially attractive.
Bipolar cell–photoreceptor connectivity in the zebrafish (Danio rerio) retina

Bipolar cells convey luminance, spatial, and color information from photoreceptors to amacrine and ganglion cells. We studied the photoreceptor connectivity of 321 bipolar cells in the adult zebrafish retina. 1,1′‐Dioctadecyl‐3,3,3′,3′‐tetramethylindocarbocyanine perchlorate (DiI) was inserted into whole‐mounted transgenic zebrafish retinas to label bipolar cells. The photoreceptors that connect to these DiI‐labeled cells were identified by transgenic fluorescence or their positions relative to the fluorescent cones, as cones are arranged in a highly ordered mosaic: rows of alternating blue‐ (B) and ultraviolet‐sensitive (UV) single cones alternate with rows of red‐ (R) and green‐sensitive (G) double cones. Rod terminals intersperse among cone terminals. As many as 18 connectivity subtypes were observed, 9 of which—G, GBUV, RG, RGB, RGBUV, RGRod, RGBRod, RGBUVRod, and RRod bipolar cells—accounted for 96% of the population. Based on their axon terminal stratification, these bipolar cells could be further subdivided into ON, OFF, and ON–OFF cells. The dendritic spread size, soma depth and size, and photoreceptor connections of the 308 bipolar cells within the nine common connectivity subtypes were determined, and their dendritic tree morphologies and axonal stratification patterns compared. We found that bipolar cells with the same axonal stratification patterns could have heterogeneous photoreceptor connectivity whereas bipolar cells with the same dendritic tree morphology usually had the same photoreceptor connectivity, although their axons might stratify on different levels. J. Comp. Neurol. 520:3786–3802, 2012. © 2012 Wiley Periodicals, Inc.
Effect of Timely Insulin Administration on Pancreatic B-Cells of Otsuka-Long-Evans-Tokushima-Fatty (OLETF) Strain Rats

Morphological changes of the pancreas and impairment of pancreatic B-cell function in a model rat (Otsuka Long Evans Tokushima Fatty [OLETF]) with non-insulin dependent diabetes mellitus might be the result of over-activity of B-cells to compensate for insulin insensitivity. To test this possibility, we studied whether the histological and functional alterations in the pancreas of male OLETF rats were improved by treatment with insulin for a certain period, which might reduce the burden to B-cells. Groups of 6 male OLETF rats and 5 or 4 male non-diabetic control Long Evans Tokushima Otsuka (LETO) rats received injections of insulin (Ultralente MC; 10 U/kg/day to OLETF rats, 5 U/kg/day to LETO rats) or saline subcutaneously, once a day for 3 weeks from 24 weeks of age. Then their insulin responses to glucose (200 mg/dl) and arginine (10 mmol/l) were examined by perfusion of the pancreas. The morphological features of their pancreata were also examined. The insulin response to glucose in OLETF rats treated with insulin was significantly higher than that of OLETF rats treated with saline (sigma IRI 142.5 +/- 27.0 vs. 37.4 +/- 6.3 ng/ml.20 min, p < 0.05) and unlike in the latter showed the normal two phases. The morphological changes of the pancreas in the insulin-treated OLETF rats were remarkably ameliorated, animals showing no enlargement and only slight fibrosis of islets. Thus treatment with insulin was effective for preventing B-cell dysfunction and morphological changes of the pancreas in NIDDM model rats.
Perioperative risk factors in obese patients for bariatric surgery: a Singapore experience.

INTRODUCTION Morbid obesity and obstructive sleep apnoea (OSA) are increasingly encountered in anaesthetic practice today. Difficult intubation may be seen more frequently in our practice. This high-risk group may also be more prone to complications in the postoperative period. METHODS We reviewed a consecutive series of patients who had undergone laparoscopic gastric banding at our institution from 2001 to 2006. The incidence of difficult intubation, early postoperative complications and its attendant risk factors were studied. RESULTS Severe OSA and neck circumference greater than 44 cm were factors associated with difficult intubation in morbidly obese patients who presented for bariatric surgery. Asthma and increasing age may be associated risk factors for adverse events in the postoperative period. CONCLUSION It is important to anticipate and prepare for a difficult intubation scenario in patients with severe OSA and a larger neck circumference. Close monitoring is recommended for patients with respiratory comorbidities and advanced age.
A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration

ABSTRACT Fascin is an actin-binding and bundling protein that is highly upregulated in most epithelial cancers. Fascin promotes cell migration and adhesion dynamics in vitro and tumour cell metastasis in vivo. However, potential non-actin bundling roles for fascin remain unknown. Here, we show for the first time that fascin can directly interact with the microtubule cytoskeleton and that this does not depend upon fascin-actin bundling. Microtubule binding contributes to fascin-dependent control of focal adhesion dynamics and cell migration speed. We also show that fascin forms a complex with focal adhesion kinase (FAK, also known as PTK2) and Src, and that this signalling pathway lies downstream of fascin–microtubule association in the control of adhesion stability. These findings shed light on new non actin-dependent roles for fascin and might have implications for the design of therapies to target fascin in metastatic disease. Summary: Fascin associates directly with microtubules independently of F-actin binding, and this contributes to microtubule dynamics, adhesion assembly and cell migration.
PERILS OF TAX REFORM

Tax reforms dangle possibilities of improving the tax system, but are fraught with perils that are evident from the 2017 U.S. experience and caution against frequent reforms of its ilk. The first peril is that reforms containing tax provisions selected simply on the basis of their projected revenue contributions will produce less tax revenue than anticipated, illustrative calculations suggesting shortfalls of roughly 8–16 percent. The second peril is that reforms will not advance the objectives of efficiency and tax equity to the extent that they include provisions intended to influence future tax legislation or government spending. The third peril is that reforms designed without sufficient appreciation of transitional gains and losses will offer inadequate and misdirected transition relief. And the fourth peril is that reforms stoke expectations of future tax changes, discouraging investment and encouraging costly tax avoidance. Tax reforms that apply sound principles will reduce or even avoid these perils.
The Causal Impact of Women's Age at Marriage on Domestic Violence in India

We examine the causal effect of women's age at marriage on prevalence of domestic violence using newly available household data from India. We employ an empirical strategy that utilizes variation in age at menarche to obtain exogenous variation in women's age at marriage. We find robust evidence that a one-year delay in women's marriage causes a significant decline in physical violence, although it has no impact on sexual or emotional violence. Further, we show that the effect of women's marital age on physical violence arises primarily because older brides, as compared to younger brides, are more educated. Overall, our findings underscore the importance of better enforcement of existing social policies that seek to delay marriages of women, as well as formulation of newer interventions (especially those which would ensure that marriage delays are associated with women staying longer in school), to reduce the prevalence of domestic violence in developing countries.