Miracle-dz/UniFAR-FTUs huggingface dataset:
Anticancer Effects of Ganoderma lucidum: A Review of Scientific Evidence

Abstract: "Lingzhi" (Ganoderma lucidum), a popular medicinal mushroom, has been used in China for longevity and health promotion since ancient times. Investigations into the anticancer activity of lingzhi have been performed in both in vitro and in vivo studies, supporting its application for cancer treatment and prevention. The proposed anticancer activity of lingzhi has prompted its usage by cancer patients. It remains debatable as to whether lingzhi is a food supplement for health maintenance or actually a therapeutic "drug" for medical proposes. Thus far there has been no report of human trials using lingzhi as a direct anticancer agent, despite some evidence showing the usage of lingzhi as a potential supplement to cancer patients. Cellular immune responses and mitogenic reactivity of cancer patients have been enhanced by lingzhi, as reported in two randomized and one nonrandomized trials, and the quality of life of 65% of lung cancer patients improved in one study. The direct cytotoxic and anti-angiogenesis mechanisms of lingzhi have been established by in vitro studies; however, clinical studies should not be neglected to define the applicable dosage in vivo. At present, lingzhi is a health food supplement to support cancer patients, yet the evidence supporting the potential of direct in vivo anticancer effects should not be underestimated. Lingzhi or its products can be classified as an anticancer agent when current and more direct scientific evidence becomes available.
Striatal dopamine transporters and cognitive functioning in healthy men and women.

OBJECTIVE There is growing interest in distinguishing the biological bases of sex differences in behavior from environmental influences. Sex hormone levels seem to be related to some cognitive abilities, particularly memory, and the dopaminergic system participates in the mediation of memory. The dopamine transporter is the primary indicator of dopaminergic tone. This study investigated the relationship between cognition and dopamine transporter availability in healthy men and women. METHOD Dopamine transporter levels were measured with a technetium-99m radiolabeled analog of cocaine, TRODAT-1, in 66 healthy volunteers (30 men and 36 women). A neuropsychological battery designed to target functions associated with dopaminergic activity was administered during the uptake interval between the radiopharmaceutical injection and image acquisition. RESULTS Women and younger participants had higher dopamine availability in the caudate nucleus, and these groups also performed better on verbal learning tasks. Furthermore, dopamine transporter availability was correlated with learning performance within groups. Relationships between dopamine availability in the caudate and putamen and executive and motor functioning were observed in women, but not in men. CONCLUSIONS The results provide further evidence for age effects and sex differences in the neuromodulatory influences of dopamine on behavior in humans.
Spatiotemporal features of neurogenesis in the retina of medaka, Oryzias latipes

The vertebrate retina is very well conserved in evolution. Its structure and functional features are very similar in phyla as different as primates and teleost fish. Here, we describe the spatiotemporal characteristics of neurogenesis in the retina of a teleost, medaka, and compare them with other species, primarily the zebrafish. Several intriguing differences are observed between medaka and zebrafish. For example, photoreceptor differentiation in the medaka retina starts independently in two different areas, and at more advanced stages of differentiation, medaka and zebrafish retinae display obviously different patterns of the photoreceptor cell mosaic. Medaka and zebrafish evolutionary lineages are thought to have separated from each other 110 million years ago, and so the differences between these species are not unexpected, and may be exploited to gain insight into the architecture of developmental pathways. Importantly, this work highlights the benefits of using multiple teleost models in parallel to understand a developmental process. Developmental Dynamics 237:3870–3881, 2008. © 2008 Wiley‐Liss, Inc.
Hijacking of leguminous nodulation signaling by the rhizobial type III secretion system

Significance Root–nodule symbiosis between leguminous plants and rhizobia requires rhizobial Nod factors (NFs) and their leguminous receptors (NFRs). Here we show that symbiosis in the soybean rhizobium Bradyrhizobium elkanii is promoted by the type III secretion system (T3SS), which delivers virulence factors via pathogenic bacteria. Nodulation tests and expression analyses using mutants of both B. elkanii and soybean (Glycine max) revealed that rhizobial T3SS activates host nodulation signaling in the absence of NFs and NFRs. These results suggest that rhizobia have adopted a pathogenic system that stimulates their legume hosts to initiate symbiotic programs. Root–nodule symbiosis between leguminous plants and nitrogen-fixing bacteria (rhizobia) involves molecular communication between the two partners. Key components for the establishment of symbiosis are rhizobium-derived lipochitooligosaccharides (Nod factors; NFs) and their leguminous receptors (NFRs) that initiate nodule development and bacterial entry. Here we demonstrate that the soybean microsymbiont Bradyrhizobium elkanii uses the type III secretion system (T3SS), which is known for its delivery of virulence factors by pathogenic bacteria, to promote symbiosis. Intriguingly, wild-type B. elkanii, but not the T3SS-deficient mutant, was able to form nitrogen-fixing nodules on soybean nfr mutant En1282. Furthermore, even the NF-deficient B. elkanii mutant induced nodules unless T3SS genes were mutated. Transcriptional analysis revealed that expression of the soybean nodulation-specific genes ENOD40 and NIN was increased in the roots of En1282 inoculated with B. elkanii but not with its T3SS mutant, suggesting that T3SS activates host nodulation signaling by bypassing NF recognition. Root-hair curling and infection threads were not observed in the roots of En1282 inoculated with B. elkanii, indicating that T3SS is involved in crack entry or intercellular infection. These findings suggest that B. elkanii has adopted a pathogenic system for activating host symbiosis signaling to promote its infection.
More on Marriage, Fertility, and the Distribution of Income

According to Pareto, the distribution of income depends on "the nature of the people comprising a society, on the organization of the latter, and, also, in part, on chance. " An overlapping generations model of marriage, fertility and income distribution is developed here. The "nature of the people" is captured by attitudes toward marriage, divorce,fertility, and children. Singles search for mates in a marriage market. They are free to accept or reject marriage proposals. Married agents make their decisions through bargaining about work, and the quantity and quality of children. They can divorce. Social policies, such as child tax credits or child support requirements, reflect the "organization of the (society). " Finally, "chance" is modeled by randomness in income, opportunities for marriage, and marital bliss.
Comparison of Single Genome and Allele Frequency Data Reveals Discordant Demographic Histories

Inference of demographic history from genetic data is a primary goal of population genetics of model and nonmodel organisms. Whole genome-based approaches such as the pairwise/multiple sequentially Markovian coalescent methods use genomic data from one to four individuals to infer the demographic history of an entire population, while site frequency spectrum (SFS)-based methods use the distribution of allele frequencies in a sample to reconstruct the same historical events. Although both methods are extensively used in empirical studies and perform well on data simulated under simple models, there have been only limited comparisons of them in more complex and realistic settings. Here we use published demographic models based on data from three human populations (Yoruba, descendants of northwest-Europeans, and Han Chinese) as an empirical test case to study the behavior of both inference procedures. We find that several of the demographic histories inferred by the whole genome-based methods do not predict the genome-wide distribution of heterozygosity, nor do they predict the empirical SFS. However, using simulated data, we also find that the whole genome methods can reconstruct the complex demographic models inferred by SFS-based methods, suggesting that the discordant patterns of genetic variation are not attributable to a lack of statistical power, but may reflect unmodeled complexities in the underlying demography. More generally, our findings indicate that demographic inference from a small number of genomes, routine in genomic studies of nonmodel organisms, should be interpreted cautiously, as these models cannot recapitulate other summaries of the data.
Electrophysiology of a slow (0.5‐4 Hz) intrinsic oscillation of cat thalamocortical neurones in vivo.

1. Electrophysiologically identified thalamocortical neurones have been intra‐ and extracellularly recorded in acutely prepared cats, under different anaesthetic conditions. 2. A slow (0.5‐4 Hz) membrane potential oscillation was observed in thalamocortical cells recorded in motor, sensory, associational and intralaminar thalamic nuclei. The oscillation consisted of rhythmic low‐threshold spikes alternating with after‐hyperpolarizations. 3. About 80% of the neurones with intact cortical connections were set into the slow oscillatory mode by bringing their membrane potential to between ‐68 and ‐90 mV. The oscillation did not depend upon the occurrence of fast action potentials and did not outlast the imposed hyperpolarization. 4. Anatomical or functional disconnection from related cortical areas resulted in a membrane potential hyperpolarization of about 9 mV and in the occurrence of spontaneous slow oscillations in virtually all recorded neurones. The intrinsic nature of the phenomenon was supported by the lack of rhythmic postsynaptic potentials as the cells were prevented from oscillating by outward current injection. 5. In contrast with other thalamic nuclei, the slow oscillation has not been observed in anterior thalamic neurones despite their having similar basic electrophysiological properties. 6. Barbiturate administration suppressed the slow oscillatory mode, an effect accompanied by a decrease in the membrane input resistance. 7. Multiunit recordings of spontaneously oscillating cells showed epochs characterized by phase‐related firing. This synchronous discharge was paralleled by a clear‐cut build‐up of field potentials in the frequency range of electroencephalogram slow or delta waves. 8. These results demonstrate that the majority of thalamocortical neurones are endowed with electrophysiological properties allowing them to oscillate at 0.5‐4 Hz, if they have a membrane potential more negative than ‐65 mV and a high input resistance. Such a condition is physiologically achieved in the deepest stages of electroencephalogram‐synchronized sleep, as a result of brain stem‐thalamic as well as cortico‐thalamic deafferentation. We postulate a thalamic contribution in the genesis of electroencephalogram delta waves during slow wave sleep, once independently oscillating thalamocortical cells become in phase.
The Role of Formin Tails in Actin Nucleation, Processive Elongation, and Filament Bundling*

Background: Formins build essential actin-based structures. Results: The tails of Cappuccino and other formins contribute to both nucleation and processive filament elongation by binding actin monomers and filaments, respectively. Conclusion: Formin tails tune actin assembly. Their role in processivity was not previously recognized. Significance: Identifying the functions of the tail domain will lead to an understanding of how Capu and other formins function and are regulated. Formins are multidomain proteins that assemble actin in a wide variety of biological processes. They both nucleate and remain processively associated with growing filaments, in some cases accelerating filament growth. The well conserved formin homology 1 and 2 domains were originally thought to be solely responsible for these activities. Recently a role in nucleation was identified for the Diaphanous autoinhibitory domain (DAD), which is C-terminal to the formin homology 2 domain. The C-terminal tail of the Drosophila formin Cappuccino (Capu) is conserved among FMN formins but distinct from other formins. It does not have a DAD domain. Nevertheless, we find that Capu-tail plays a role in filament nucleation similar to that described for mDia1 and other formins. Building on this, replacement of Capu-tail with DADs from other formins tunes nucleation activity. Capu-tail has low-affinity interactions with both actin monomers and filaments. Removal of the tail reduces actin filament binding and bundling. Furthermore, when the tail is removed, we find that processivity is compromised. Despite decreased processivity, the elongation rate of filaments is unchanged. Again, replacement of Capu-tail with DADs from other formins tunes the processive association with the barbed end, indicating that this is a general role for formin tails. Our data show a role for the Capu-tail domain in assembling the actin cytoskeleton, largely mediated by electrostatic interactions. Because of its multifunctionality, the formin tail is a candidate for regulation by other proteins during cytoskeletal rearrangements.
Analysis and Performance Enhancement of Vector-Controlled VSC in HVDC Links Connected to Very Weak Grids

Voltage source converter (VSC)-based high-voltage direct current (HVDC) transmission systems have been employed widely in recent years. However, connecting a VSC-HVDC link to a very weak grid (a high-impedance grid) is challenging. A vector-controlled VSC is incapable of injecting/absorbing its maximum theoretical active power in such grids. A simple yet effective control system for a standard vector-controlled VSC in a very weak grid condition has not been reported in the literature. This paper, benefiting from a comprehensive small-signal model, presents a detailed analysis of the VSC dynamics and shows how the assumptions made for designing VSC regulators in strong grids are no longer valid in very weak grids. The paper then proposes and compares two straightforward solutions: retuning the control parameters and using an artificial bus for converter-grid synchronization. Both methods enable the VSC to operate at the maximum theoretical active power at a very weak grid condition (i.e., at unity short-circuit ratio) by minimal modification in the widely accepted vector control method. The advantages and disadvantages of each method are discussed. The analytical results are verified by detailed nonlinear time-domain simulation results.
Functional Analysis of the Stability Determinant AlfB of pBET131, a Miniplasmid Derivative of Bacillus subtilis (natto) Plasmid pLS32

ABSTRACT Bacillus subtilis plasmid pBET131 is a derivative of pLS32, which was isolated from a natto strain of Bacillus subtilis. The DNA region in pBET131 that confers segregational stability contains an operon consisting of three genes, of which alfA, encoding an actin-like ATPase, and alfB are essential for plasmid stability. In this work, the alfB gene product and its target DNA region were studied in detail. Transcription of the alf operon initiated from a σA-type promoter was repressed by the alfB gene product. Overproduction of AlfA was inhibitory to cell growth, suggesting that the repression of the alf operon by AlfB is important for maintaining appropriate levels of AlfA. An electrophoretic mobility shift assay and footprinting analysis with purified His-tagged AlfB showed that it bound to a DNA region containing three tandem repeats of 8-bp AT-rich sequence (here designated parN), which partially overlaps the −35 sequence of the promoter. A sequence alteration in the first or third repeat did not affect the AlfB binding and plasmid stability, whereas that in the second repeat resulted in inhibition of these phenomena. The repression of alfA-lacZ expression was observed in the constructs carrying a mutation in either the first or third repeat, but not in the second repeat, indicating a correlation between plasmid stability, AlfB binding, and repression. It was also demonstrated by the yeast two-hybrid system that AlfA and AlfB interact with each other and among themselves. From these results, it was concluded that AlfB participates in partitioning pBET131 by forming a complex with AlfA and parN, the mode of which is typified by the type II partition mechanism.
The Effects of Retrospective Household Income on Neurocognitive Disorders: Results from Project Frontier Data

The relationship between health status and socioeconomic status (SES) has been well known. Having a disease or illness is not only a medical or biological process but also a social outcome [1-4]. SES can be measured by several variables such as education, income, and occupation. For instance, an individual is more likely to use brain function, which inhibits to develop neurocognitive disorder, when a person has more education and/or higher occupational status [5-8]. Several studies reported a negative correlation between SES and cognitive disorders, whereby the higher the SES, the lower the chance of being diagnosed with a cognitive disorder [7,9,10]. This paper utilized household income as an SES index. Household or individual income is not thoroughly investigated in terms of their relationship with neurocognitive disorders [10,11]. Income may not be directly connected to brain use, yet it has significant relationship with lifestyle, health insurance and health cost coverage, all of which have a documented impact on health status [10,12].
An Analysis of the Methodologies adopted by CSR Rating Agencies

Purpose This paper aims to analyse the similarities and differences in the methodologies adopted by corporate social responsibility (CSR) rating agencies. Design/methodology/approach The authors gather secondary and primary evidences of practices from selected agencies on the methodologies and criteria they rely upon to assess a firm’s CSR performance. Findings The authors find not only evidence of similarities in the methodologies adopted by the CSR rating agencies (e.g. the use of environment, social and governance themes, exclusion criteria, adoption of positive criteria, client/“customised” input, quantification) but also several elements of differences, namely, in terms of the thresholds for exclusion, transparent vs confidential approach, industry-specific ratings and weights for each dimension. Drawing from Sandberg et al.’s (2009) conceptualisations, the authors tentatively argue that this mixed picture may reflect competing organisational pressures to adopt a differentiation approach at the strategic and practical levels whilst recognising, and incorporating, the “globalising” tendencies of the CSR business at the terminological levels. Social implications Although these data are based on a relatively small number of agencies, the findings and analysis convey some implications for users of CSR ratings and policymakers, particularly in light of the recent Paris 2016 Agreement on Climate Change and the increased emphasis on the monitoring of social, environmental and governance performance. Originality/value The authors contribute to the literature by highlighting how key intermediate rating organisations operationalise notions of CSR.
Overexpression of Several Arabidopsis Histone Genes Increases Agrobacterium-Mediated Transformation and Transgene Expression in Plants[W]

The Arabidopsis thaliana histone H2A-1 is important for Agrobacterium tumefaciens–mediated plant transformation. Mutation of HTA1, the gene encoding histone H2A-1, results in decreased T-DNA integration into the genome of Arabidopsis roots, whereas overexpression of HTA1 increases transformation frequency. To understand the mechanism by which HTA1 enhances transformation, we investigated the effects of overexpression of numerous Arabidopsis histones on transformation and transgene expression. Transgenic Arabidopsis containing cDNAs encoding histone H2A (HTA), histone H4 (HFO), and histone H3-11 (HTR11) displayed increased transformation susceptibility, whereas histone H2B (HTB) and most histone H3 (HTR) cDNAs did not increase transformation. A parallel increase in transient gene expression was observed when histone HTA, HFO, or HTR11 overexpression constructs were cotransfected with double- or single-stranded forms of a gusA gene into tobacco (Nicotiana tabacum) protoplasts. However, these cDNAs did not increase expression of a previously integrated transgene. We identified the N-terminal 39 amino acids of H2A-1 as sufficient to increase transient transgene expression in plants. After transfection, transgene DNA accumulates more rapidly in the presence of HTA1 than with a control construction. Our results suggest that certain histones enhance transgene expression, protect incoming transgene DNA during the initial stages of transformation, and subsequently increase the efficiency of Agrobacterium-mediated transformation.
Operative Treatment of Adolescent Idiopathic Scoliosis With Posterior Pedicle Screw-Only Constructs: Minimum Three-Year Follow-up of One Hundred Fourteen Cases

Study Design. Preoperative review of a prospective study, single institution, consecutive series. Objective. To analyze the intermediate-term follow-up of consecutive adolescent idiopathic scoliosis (AIS) patients treated with pedicle screw constructs. Summary of Background Data. There have been no reports of the intermediate-term findings in North America following posterior spinal fusion with the use of pedicle screw-only constructs. Methods. One hundred and fourteen consecutive patients having a minimum 3-year follow-up (mean 4.8 ± 1.1; range, 3.0–7.3 years) with AIS were evaluated. The average age at surgery was 14.9 ± 2.2 years. Radiographic measurements included preoperative (Preop), postoperative (PO), 2-year (2 years), and final follow-up (FFU). A chart review evaluated PFTs, Scoliosis Research Society scores, presence of thoracoplasty, Risser sign, Lenke classification, and complications. Results. The most frequent curve pattern was Lenke type 1 (45.6%), followed by type 3 (21.9%). The average main thoracic curve measured 59.2° ± 12.2 SD Preop, and corrected to 16.8° ± 9.9 PO (P < 0.0001). Sagittal thoracic alignment (T5–T12) decreased from 25.8° to 15.5° at FFU (P = 0.05). Nash-Moe grading for apical vertebral rotation (AVR) in the proximal thoracic curve decreased from 2.0 Preop to 1.1 at FFU (P < 0.0001), and AVR in the thoracolumbar/lumbar spine decreased from 1.6 Preop to 1.1 at FFU (P < 0.0001). Importantly, the horizontalization of the subjacent disc measured −8.3° Preop which decreased to −0.9° PO (P < 0.001). PFT follow-up averaged 2.4 years with a 7.1% improvement in FVC (P = 0.004) and 8.8% in FEV1 (P < 0.0001). SRS scores averaged 83.0% at latest follow-up. Age, gender, Risser sign, or complications did not have a significant effect on outcomes. There were 2 cases of adding-on, 3 late onset infections, 1 with a single pseudarthrosis, but no neurologic complications. Conclusion. This is the largest (N = 114), consecutive series of North American patients with AIS treated with pedicle screws having a minimum of 3-year follow-up. The average curve correction was 68% for the main thoracic, 50% for the proximal thoracic, and 66% for the thoracolumbar/lumbar curve at final follow-up.