Miracle-dz/UniFAR-FTUs huggingface dataset:
Effect of renal nerves on expression of renin and angiotensinogen genes in rat kidneys.

In this study, we try to determine the influence of renal nerve activity on renal function, plasma renin activity (PRA), and the corresponding expression of renin and angiotensinogen genes in the kidney. In pentobarbitone-anesthetized rats, the left renal nerves were stimulated (15 V, 0.2 ms) at frequencies to reduce left renal blood flow by 15, 30, and 45%. There were corresponding reductions in glomerular filtration rate from 12 to 52% and absolute and fractional sodium excretions from 20 to 75%. PRA levels in control rats were 10.8 +/- 1.5 and were increased to 65.9 +/- 9.1, 144.2 +/- 19.7, and 277.2 +/- 22.0 ng angiotensin I.h-1.ml-1 after 1 h at each of the three levels of nerve stimulation. Renal renin mRNA was similar in innervated and denervated kidneys and was not affected by the lowest level of nerve stimulation; however, neurally induced decreases in blood flow to 30 and 45% increased renin mRNA levels by 3.0- and 3.4-fold (both P < 0.05), respectively. Angiotensinogen mRNA levels were higher (P < 0.05) in kidneys subjected to the lowest level of nerve stimulation, but when renal blood flow was reduced by 30 and 45%, expression of this gene was unchanged. Stimulation of the renal nerves in the presence of the beta 1-adrenoceptor antagonist atenolol only doubled PRA at the highest rates of stimulation. Neither renal renin nor angiotensinogen mRNA were changed during neurally mediated reductions in renal blood flow of 15 or 30% after administration of atenolol. (ABSTRACT TRUNCATED AT 250 WORDS)
Tourism Policy and Governance for Development

This chapter proposes a methodology to determine tourism policies that are effective in addressing the challenges of tourism as an instrument for development. A three-step process is proposed, including the preparation of a Green Paper that defines the different actors in the tourism system, as well as their functions vis-a-vis policy options; a White Paper that determines strategic positioning and a roadmap for action based on the diagnosis and analysis of the destination; and a Tourism Policy Plan that delineates the different governance actions. The model is examined from the perspective of the use of tourism as an instrument for development, with a consideration of the destination’s human, social capital, and participative governance systems.
Anaemia, iron deficiency and vitamin A status among school-aged children in rural Kazakhstan

Abstract Objectives To investigate the prevalence of anaemia and iron deficiency and vitamin A status among school-aged children in rural Kazakhstan and identify factors associated with anaemia in this population. Design A cross-sectional design. Setting School-aged children in rural Kazakhstan. Subjects Socio-economic and anthropometric information was collected from 159 school-aged children living in the Kzyl-Orda region of Kazakhstan. Blood samples were collected and the concentrations of haemoglobin (Hb), serum iron, serum ferritin (SF), erythrocyte protoporphyrin (EP), serum retinol and β-carotene, total iron binding capacity (TIBC), transferrin saturation (TS) and other haematological indices were measured. Results Among the 159 children, the prevalence of anaemia and iron deficiency defined by the multiple criteria model (SF, TS and EP) was 27% and 13%, respectively. Nine per cent had iron-deficiency anaemia and 21% had serum retinol value <1.05 μmol l−1. Mean SF and serum iron concentrations and TS were significantly lower in anaemic children than in their non-anaemic peers, while TIBC and EP were significantly higher in children with anaemia. Hb was significantly correlated with serum iron and retinol concentrations. Serum retinol and SF concentrations and mean corpuscular volume were significantly correlated with Hb by multiple regression analysis. Conclusions Anaemia among school-aged children in rural Kazakhstan appears to be related to iron indices and vitamin A status.
Pathological investigations on guillemots (Uria aalge) stranded on the Belgian coast during the winter of 1993–94

Pathological investigations were carried out on 67 guillemots (Uria aalge) washed up on the Belgian coast between November 1993 and March 1994. Emaciation and acute haemorrhagic gastroenteropathy were observed in more than 70 per cent of the birds. There was no statistical relationship between the level of oil contamination and the severity of the lesions. Differences in bodyweight were accounted for by age, sex, emaciation, and acute haemorrhagic gastroenteropathy. The birds had a severe weight deficit but the concentrations of pollutants were below acutely toxic levels.
Functional analysis of the sialyltransferase complexes in Escherichia coli K1 and K92

The polysialyltransferase (polyST) structural gene, neuS, for poly alpha 2,8sialic acid (PSA) capsule synthesis in Escherichia coli K1 was previously mapped near the kps region 1 and 2 junction (S. M. Steenbergen and E. R. Vimr, Mol. Microbiol. 4:603-611, 1990). Present Southern and colony blot hybridization results confirmed that neuS was a region 2 locus and indicated apparent homology with neuS from E. coli K92, bacteria that synthesize a sialyl alpha 2,8-2,9-linked polymer. A K1- mutant with an insertion mutation in neuS was complemented in trans by K92 neuS, providing direct evidence that neuS encoded the PSA polymerase. A 2.9-kb E. coli K1 kps subclone was sequenced to better characterize polyST. In addition to neuS, the results identified a new open reading frame, designated neuE, the linker sequence between regions 1 and 2, and the last gene of region 1, kpsS. The kpsS translational reading frame was confirmed by sequencing across the junction of a kpsS'-lacZ+ fusion. PolyST was identified by maxicell analysis of nested deletions and coupled in vitro transcription-translation assays. PolyST's derived primary structure predicted a 47,500-Da basic polypeptide without extensive similarity to other known proteins. PolyST activity was increased 31-fold and was membrane localized when neuS was cloned into an inducible expression vector, suggesting, together with the polyST primary structure, that polyST is a peripheral inner membrane glycosyltransferase. However, polyST could not initiate de novo PSA synthesis, indicating a functional requirement for other kps gene products. The existence of a sialyltransferase distinct from polyST was suggested by identification of a potential polyprenyl-binding motif in a C-terminal membrane-spanning domain of the predicted neuE gene product. Direct evidence for a quantitatively minor sialyltransferase activity, which could function to initiate PSA synthesis, was obtained by phenotypic analysis of mutants with multiple defects in sialic acid synthesis, degradation, and polymerization. The results provide an initial molecular description of K1 and K92 sialyltransferase complexes and suggest a possible common function for accessory kps gene products.
Factors Associated With Noncompletion of Latent Tuberculosis Infection Treatment: Experience From the PREVENT TB Trial in the United States and Canada.

BACKGROUND Overall rates of noncompletion of treatment (NCT) for latent tuberculosis infection (LTBI) in the PREVENT TB trial were 18% for 3 months of directly observed once-weekly rifapentine (maximum dose, 900 mg) plus isoniazid (maximum dose, 900 mg) (3HP-DOT) and 31% for 9 months of daily self-administered isoniazid (maximum dose, 300 mg; 9H-SAT). NCT for LTBI reduces its effectiveness. The study objective was to assess factors associated with NCT for LTBI among adult participants enrolled at US and Canadian sites of the PREVENT TB trial. METHODS This was a post hoc exploratory analysis of the randomized, open-label PREVENT TB trial. Factors were analyzed by univariate and multivariate logistic regression (with enrollment site as a random effect). RESULTS From 6232 participants analyzed, 1406 (22.6%) did not complete LTBI treatment (317 NCT attributed to an adverse event [NCT-AE] and 1089 NCT attributed to reasons other than an adverse event [NCT-O]). The proportion of NCT-AE was similar with both regimens (3HP-DOT = 6.4% vs 9H-SAT = 5.9%; P = .23); NCT-O was higher among participants enrolled in 9H-SAT (9H-SAT = 24.5% vs 3HP-DOT = 12.7%; P = .02). Among those in the NCT-AE group, being non-Hispanic and receiving 3HP-DOT, having cirrhosis and receiving 9H-SAT, alcohol consumption among men, and use of concomitant medication were associated with NCT-AE. Among those in the NCT-O group, receiving 9H-SAT, missing ≥1 early visit, men receiving 9H-SAT, men with a history of incarceration, alcohol abuse, use ever of intravenous drugs, younger age receiving 9H-SAT, and smoking were associated with NCT-O. CONCLUSIONS Factors associated with NCT, such as missing a clinic visit early during treatment, might help identify persons for whom tailored interventions could improve completion of LTBI treatment. CLINICAL TRIALS REGISTRATION NCT00023452.
Ship target recognition using high resolution range profiles based on FMT and SVM

This paper presented here introduces a technique for automatic recognition of ship targets from high range resolution range profiles. The technique incorporates Fourier-Mellin transform (FMT) and support vector machine (SVM) to identify ship targets. According to the shift invariance of the Fourier transform and scale invariance of the Mellin transform, the high resolution range profiles are processed using FMT to form the feature vectors. Within the recognizer, the multi-class SVM is used based on binary tree, which can reduce the number of SVM classifiers and repetitive training samples effectively. The recognition results from simulation data and measurement data of ship target range profiles prove the validity and robustness of the method proposed.
Microfluidic Quantitative PCR for Simultaneous Quantification of Multiple Viruses in Environmental Water Samples

ABSTRACT To secure food and water safety, quantitative information on multiple pathogens is important. In this study, we developed a microfluidic quantitative PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, human norovirus, rotavirus, sapovirus, and hepatitis A and E viruses. Murine norovirus and mengovirus were also quantified in our MFQPCR system as a sample processing control and an internal amplification control, respectively. River water contaminated with effluents from a wastewater treatment plant in Sapporo, Japan, was collected and used to validate our MFQPCR system for multiple viruses. High-throughput quantitative information was obtained with a quantification limit of 2 copies/μl of cDNA/DNA. Using this MFQPCR system, we could simultaneously quantify multiple viral pathogens in environmental water samples. The viral quantities obtained using MFQPCR were similar to those determined by conventional quantitative PCR. Thus, the MFQPCR system developed in this study can provide direct and quantitative information for viral pathogens, which is essential for risk assessments.
Personality in DSM–5: Helping Delineate Personality Disorder Content and Framing the Metastructure

The transition from the Diagnostic and Statistical Model of Mental Disorders (4th ed., text revision [DSM–IV–TR]; American Psychiatric Association, 2000) to the fifth edition (DSM–5) represents an unprecedented opportunity to integrate dimensional personality trait models into the official nosology. Not surprisingly, a variety of issues have arisen in contemplating this challenging integration. In this article, we address how a dimensional personality trait model could be a helpful component of DSM–5, from the perspective of our roles as work group members and advisors involved in the creation of a trait model and corresponding assessment instrument. We focus in particular on two potential roles for a trait model in DSM–5 that are under official consideration. First, a dimensional personality trait model might be helpful in delineating the content of personality disorders. Second, a trait model might assist in organizing the “metastructure” of DSM–5 (i.e., the arrangement of chapters and other broader classificatory rubrics).
Using Skin Models to Assess the Effects of a Protection Cream on Skin Barrier Function

Background: There is a basic necessity to understand the mechanisms of the protective effects of emulsions. This would promote the development of protective cosmetics and therefore improve the prevention and treatment of occupational skin diseases. However, for such studies, no reliable skin model is available. Objective: An in vitro skin model test was developed to evaluate the protective mechanism of cosmetic ingredients. Methods: The efficacy of three products was assessed by an in vivo test (Repetitive Occlusive Irritation Test) and then 3-dimensional skin model tests were carried out. Results: In vivo test results demonstrate that the best protection against sodium dodecyl sulphate is offered by a multiple emulsion. In the case of a skin model test, sodium dodecyl sulphate led to cell damage, an increase in pro-inflammatory markers and some barrier lipids. The multiple emulsion increased the content of skin lipids, without inducing irritation or cell death. Conclusion: Skin models react similarly to sodium dodecyl sulphate compared to human skin and therefore they are suitable to study barrier repair after sodium dodecyl sulphate damage. It is likely that the superior protective effect of the multiple emulsion in vivo is based on the increased amount of skin barrier lipids.
Climate Change and Gender Justice: International Policy and Legal Responses

Climate change raises issues of justice for different subjects of law – states and individuals. It is therefore not surprising that international policy and legal responses to climate change took equity concerns on board by considering differentiated responsibilities for climate change and taking respective capabilities of states into account in assigning the role to protect the climate system for the benefit of present and future generations of humankind. While the link between gender and climate change has not always been obvious, there is increasing evidence to demonstrate that women and men experience climate change differently; that climate change increases women’s vulnerability; and gender inequalities worsen women’s coping capacities. This article looks at the relationship between gender and climate change and how international policies and laws on gender and climate change address the interface. It also highlights the increasing advocacy for the inclusion of gender justice in international climate change debates. It concludes that including gender in the laws, policies and discussions on climate change brings a critical constituency to these platforms and also enhances the effectiveness of the interventions aimed at dealing with climate change because of the roles that women play in different programmes and contexts.
Industrial ecology and eco-industrial development: case studies from Italy

Eco-industrial parks (EIPs) are becoming a way to guarantee a sustainable development of productive areas. Many scholars describe the main features of EIPs around the world contributing to the update of technological best practices and management options for these areas. Despite this, the Italian experiences on EIPs are not so well described in the literature while in Italy is present a fervent policy and industrial situation on this matter. The paper aims to bridge this gap describing the Italian most advanced experiences in the field of eco-industrial development. The paper aims at enriching the international knowledge on EIPs including technical data and the main features of Italian case studies and compare these with some international experiences.
On capacity optimization in multi-radio multi-channel wireless networks with directional antennas

Exploiting multiple radio interfaces over multiple channels and using directional antennas are promising technologies to enhance the performance of wireless networks. However, in such a multi-dimensional network resource space, assignment of radios and channels and configuration of antenna directions are coupled, making the complexity for network capacity optimization dramatically increase. Existing work has considered either multi-radio multi-channel (MRMC) networks or networks with directional antennas (DA); however, there lacks a generic framework for such complex MRMC-DA wireless networks. In this paper, we employ the tuple concept to define Link- Radio-Antenna-Channel tuple links, which are then utilized as building blocks to construct a multi-dimensional conflict graph (MDCG) of the MRMC-DA network. The MDCG model facilitates mapping the original MRMC-DA network into a simple virtual single-radio single-channel network, on which the capacity optimization problem can be formulated as a linear program. To circumvent searching the exponentially many independent sets, we apply the delayed column generation method to design our algorithm. Simulations demonstrate the performance of the proposed method and analyze the different effects of the numbers of channels, radios and antenna choices.
Dual regulating effects of gastrodin on extracellular dopamine concentration in rats models of Tourette's syndrome

Purpose: Evaluate the dual regulating effects of gastrodin on striatal extracellular dopamine (DA) concentration in Tourette's syndrome (TS) rat models, and explore the underlying pharmacological mechanisms. Materials and Methods: Seventy Wistar rats were randomly divided into control group and TS model group. The former was intraperitoneally injected with saline (0.9%), while in the later, the rats were injected with Apomorphine (Apo) and 3,3’-iminodipropionitrile (IDPN) respectively to manipulate two kinds of TS rat models. Both Apo and IDPN induced rats were further assigned to three conditions, and the related rats were treated respectively by oral gavage with saline, gastrodin and Haloperidol (Hal). Data of stereotypy of the rats were collected. After 8 weeks, the extracellular content of DA and HVA in striatum were examined by intracerebral microdialysis and follow-up high-performance liquid chromatography (HPLC), and the expression of dopamine transporter (DAT) was probed by Western blot. Results: Gastrodin improved the stereotyped behaviors in TS rats. Furthermore, it down-regulated the elevated striatal extracellular DA concentration in Apo-induced rats and up-regulated the decreased DA content in the rats exposed to IDPN. Meanwhile, a dramatic down-regulation was detected in DAT protein expression in Apo + GAS group, while an opposite profile was showed in the IDPN + GAS group. Conclusions: The dual regulating effects of gastrodin on extracellular DA level have been established, and the related mechanisms would be the dual regulating effects of gastrodin on the expression of DAT, a glycoprotein in the regulation of the extracellular DA concentration.
Plutonium--health implications for man. Some problems in measuring plutonium in the environment.

Excellent analytical procedures are available for measuring plutonium in all kinds of environmental samples. Conditions are discussed under which complete sample decomposition and exchange with isotopic tracers are obtained, thus ensuring accuracy in the measurements. The major problems arise in obtaining sufficient samples to be statistically representative of the populations involved and in obtaining homogeneous samples for analysis. (auth)
Cell membrane potential and resistance in liver.

1. Isolated segments of mouse liver were placed in a Perspex bath through which physiological saline solutions of varying composition were circulated. Two microelectrodes were inserted in different liver cells under microscopic control allowing measurement of distance between the two micro‐electrode tips. Current pulses were injected through one of these electrodes, causing electrotonic potential changes in nearby cells by current spread through intercellular junctions. These electrotonic potential changes were recorded with the second micro‐electrode. The spatial decrement of the amplitude of the electrotonzpotential changes and their dependence on extracellular ion concentrations were analysed by three‐dimensional cable analysis, modified to account for the geometry of the tissue. 2. During exposure to control solution the mean resting cell membrane potential was ‐37 mV, the space constant for intracellular current spread (lambda3 = square root of Rm/chrRi) was 390 micron and Ri, a measure which includes the intracellular resistivity and the junctional resistances, was 1.4 komegacm. From these values, and an estimate of tissue cell membrane density (chi) obtained by others, the specific membrane resistance (Rm) was calculated to be 5.1 komegacm2. 3. Replacement of extracellular Na+ by K+ resulted in a large depolarization and a large decrease in the membrane resistance. Replacement of extracellular Na+ by choline resulted in a small transient hyperpolarization and a small increase in the membrane resistance. Replacement of extracellular Cl‐ by methylsulphate or sulphate or of NaCl by sucrose resulted in a small transient depolarization and a large increase in the membrane resistance. 4. Glucagon (10(‐7) M) and adrenaline (10(‐5) M) evoked membrane hyperpolarization and reduction of membrane resistance (Rm). 5. The resting membrane ion conductance can be considered to consist of three components, Cl conductance (GCl), GK and GNa. The results suggest that GCl greater than GK greater than GNa. Changes in extracellular ion concentrations specifically alter the permeability properties of the cell membrane. The glucagon action can be explained in part by an increase in GK.
Mitigation of topological inconsistency attacks in RPL‐based low‐power lossy networks

The RPL is a routing protocol for low‐power and lossy networks. A malicious node can manipulate header options used by RPL to create topological inconsistencies, thereby causing denial of service attacks, reducing channel availability, increasing control message overhead, and increasing energy consumption at the targeted node and its neighborhood. RPL overcomes these topological inconsistencies via a fixed threshold, upon reaching which all subsequent packets with erroneous header options are ignored. However, this threshold value is arbitrarily chosen, and the performance can be improved by taking into account network characteristics. To address this, we present a mitigation strategy that allows nodes to dynamically adapt against a topological inconsistency attack based on the current network conditions. Results from our experiments show that our approach outperforms the fixed threshold and mitigates these attacks without significant overhead. Copyright © 2015 John Wiley & Sons, Ltd.