Miracle-dz/UniFAR-FTUs huggingface dataset:
Efficiency of Phage φ6 for Biocontrol of Pseudomonas syringae pv. syringae: An in Vitro Preliminary Study

Pseudomonas syringae is a plant-associated bacterial species that has been divided into more than 60 pathovars, with the Pseudomonas syringae pv. syringae being the main causative agent of diseases in a wide variety of fruit trees. The most common treatments for biocontrol of P. syringae pv. syringae infections has involved copper derivatives and/or antibiotics. However, these treatments should be avoided due to their high toxicity to the environment and promotion of bacterial resistance. Therefore, it is essential to search for new approaches for controlling P. syringae pv. syringae. Phage therapy can be a useful alternative tool to the conventional treatments to control P. syringae pv. syringae infections in plants. In the present study, the efficacy of bacteriophage (or phage) φ6 (a commercially available phage) was evaluated in the control of P. syringae pv. syringae. As the plants are exposed to the natural variability of physical and chemical parameters, the influence of pH, temperature, solar radiation and UV-B irradiation on phage φ6 viability was also evaluated in order to develop an effective phage therapy protocol. The host range analysis revealed that the phage, besides its host (P. syringae pv. syringae), also infects the Pseudomonas syringae pv. actinidiae CRA-FRU 12.54 and P. syringae pv. actinidiae CRA-FRU 14.10 strains, not infecting strains from the other tested species. Both multiplicities of infection (MOIs) tested, 1 and 100, were effective to inactivate the bacterium, but the MOI 1 (maximum reduction of 3.9 log CFU/mL) was more effective than MOI 100 (maximum reduction of 2.6 log CFU/mL). The viability of phage φ6 was mostly affected by exposure to UV-B irradiation (decrease of 7.3 log PFU/mL after 8 h), exposure to solar radiation (maximum reduction of 2.1 PFU/mL after 6 h), and high temperatures (decrease of 8.5 PFU/mL after 6 days at 37 °C, but a decrease of only 2.0 log PFU/mL after 67 days at 15 °C and 25 °C). The host range, high bacterial control and low rates of development of phage-resistant bacterial clones (1.20 × 10−3) suggest that this phage can be used to control P. syringae pv. syringae infections in plants, but also to control infections by P. syringae pv. actinidiae, the causal agent of bacterial canker of kiwifruit. Although the stability of phage φ6 was affected by UV-B and solar radiation, this can be overcome by the application of phage suspensions at the end of the day or at night.
Endogenous fluctuations in the dopaminergic midbrain drive behavioral choice variability

Significance Humans are surprisingly inconsistent in their behavior, often making different choices under identical conditions. Previous research suggests that intrinsic fluctuations in brain activity can influence low-level processes, such as the amount of force applied in a motor response. Here, we show that intrinsic prestimulus brain activity in the dopaminergic midbrain influences how we choose between risky and safe options. Using computational modeling, we demonstrate that endogenous fluctuations alter phasic responses in a decision network and thereby modulate risk taking. Our findings demonstrate that higher-order cognition is influenced by fluctuations in internal brain states, providing a physiological basis for variability in complex human behavior. Human behavior is surprisingly variable, even when facing the same problem under identical circumstances. A prominent example is risky decision making. Economic theories struggle to explain why humans are so inconsistent. Resting-state studies suggest that ongoing endogenous fluctuations in brain activity can influence low-level perceptual and motor processes, but it remains unknown whether endogenous fluctuations also influence high-level cognitive processes including decision making. Here, using real-time functional magnetic resonance imaging, we tested whether risky decision making is influenced by endogenous fluctuations in blood oxygenation level-dependent (BOLD) activity in the dopaminergic midbrain, encompassing ventral tegmental area and substantia nigra. We show that low prestimulus brain activity leads to increased risky choice in humans. Using computational modeling, we show that increased risk taking is explained by enhanced phasic responses to offers in a decision network. Our findings demonstrate that endogenous brain activity provides a physiological basis for variability in complex human behavior.
The Sibling Effect for Delinquency: Mediation by Proactive Criminal Thinking and Moderation by Age

This study tested whether the sibling delinquency effect, like the peer influence effect, is mediated by proactive (planned, calculated, and amoral) criminal thinking. Youth who completed the Offending, Crime and Justice Survey (OCJS) and had a sibling living at home were divided into an early adolescent subsample (n = 795) and a mid-adolescent subsample (n = 532) after it was determined that age moderated the effect of sibling delinquency on proactive criminal thinking and serious offending. The results of a causal mediation analysis revealed a significant pathway running from sibling delinquency at Wave 1, to proactive criminal thinking at Wave 2, to serious offending at Wave 3, but only in the early adolescent subsample. These results suggest that the sibling delinquency effect may be the result of learning proactive criminal thinking in association with a delinquent sibling while still an early adolescent.
Flexible Fixation Technique for Lisfranc Injuries

Injuries to the Lisfranc complex range from purely ligamentous disruptions to fracture-dislocations of the tarsometatarsal joint. Treatment options include closed/open reduction with percutaneous pinning, open reduction and internal fixation (ORIF), and primary arthrodesis. We present a ligament reinforcement technique utilizing a flexible fixation device for the treatment of ligamentous Lisfranc injuries. Level of Evidence: Level V, expert opinion.
Scalable synthesis of water-dispersible 2D manganese dioxide monosheets

2D nanomaterials with atomic thickness usually exhibit high specific surface areas and atom exposure rates, which are suitable for surface reaction related applications. In this study, we selected the oxalate ions as the structure-inducing agent to synthesize δ-MnO2 ultrathin nanosheets (~4.5 nm) via a facile hydrothermal method. Subsequently, an efficient exfoliation method to prepare single-layer MnO2 nanosheets (~0.9 nm) with the major exposed {0 0 1} facets was successfully developed. We found that the oxalate ions play a major role in the growth and formation of δ-MnO2 ultrathin nanosheets, and the formation process of the ultrathin structure was also investigated. The resulting single-layer MnO2 nanosheets (monosheets) with exposed {0 0 1} facets showed much higher catalytic performance for carcinogenic airborne formaldehyde, better than few-layer ultrathin nanosheets and nanoflowers with exposed {1 0 0} facets. The reasons for the high catalytic activity of MnO2 monosheets can be attributed to its higher surface areas and oxygen vacancy concentration. Moreover, the density-functional-theory (DFT) theoretical calculations showed that the oxygen vacancy in single-layer {0 0 1} facets exhibited the strongest adsorption/activation ability to O2 and H2O, which was very favorable for catalytic oxidation of formaldehyde. The synthesis strategy of ultrathin nanosheets described in this article may serve as reference and guidance for the preparation of other 2D ultrathin nanomaterials.
Intrauterine Device Insertion during Cesarean Section in Women without Prenatal Contraception Counseling: Lessons from a Country with High Cesarean Rates

Abstract Objective The moment of admission for delivery may be inappropriate for offering an intrauterine device (IUD) to women without prenatal contraception counseling. However, in countries with high cesarean rates and deficient prenatal contraception counseling, this strategy may reduce unexpected pregnancies and repeated cesarean sections. Methods This was a prospective cohort study involving 100 women without prenatal contraception counseling. Postplacental IUD was offered after admission for delivery and placed during cesarean. The rates of IUD continuation, uterine perforation, and endometritis were assessed at 6 weeks and 6 months, and the proportion of women continuing with IUD at 6 months was assessed with respect to the number of previous cesareans. Results Ninety-seven women completed the follow-up. The rate of IUD continuation was 91% at 6 weeks and 83.5% at 6 months. The expulsion/removal rate in the first 6 weeks was not different from that between 6 weeks and 6 months (9 vs 9.1%, respectively). There were 2 cases of endometritis (2.1%), and no case of uterine perforation. Among 81 women continuing with intrauterine device after 6-months, 31% had undergone only the cesarean section in which the IUD was inserted, 44% had undergone 2 and 25% had undergone 3 or more cesarean sections. Conclusion Two thirds of the women who continued with IUD at 6 months had undergone 2 or more cesarean sections. Since offering trial of labor is unusual after 2 or more previous cesareans, we believe that offering IUD after admission for delivery may reduce the risk of repeated cesarean sections and its inherent risks.
Optimal Sampling and

This paper presents sore theoretical and experimental results on optimal sampling and quantization of synthetic apsrture radar (SAR) signals. It includes a description of a derived theoretical relationship between the pixel signal-to-noise ratio of processed SAR images and the number of quantization bits per sampled signal, assuming homogeneous extended targets. With this relationship known, a solution may be realized for the problem of optimal allocation of a fixed data bitvolume (for specified surface area and resolution criterion) between the number of samples and the number of b1ts-per-sample. The results indicate that to achieve the best possible image quality for a fixed bit rate and a given resolution criterion, one should quantize individual samples coarsely and thereby maximize the number of multiple looks. The theoretical results are then compared with simulation results obtained by processing aircraft SAR data.
Role of the Microenvironment in Regulating Normal and Cancer Stem Cell Activity: Implications for Breast Cancer Progression and Therapy Response

The epithelial cells in an adult woman’s breast tissue are continuously replaced throughout their reproductive life during pregnancy and estrus cycles. Such extensive epithelial cell turnover is governed by the primitive mammary stem cells (MaSCs) that proliferate and differentiate into bipotential and lineage-restricted progenitors that ultimately generate the mature breast epithelial cells. These cellular processes are orchestrated by tightly-regulated paracrine signals and crosstalk between breast epithelial cells and their tissue microenvironment. However, current evidence suggests that alterations to the communication between MaSCs, epithelial progenitors and their microenvironment plays an important role in breast carcinogenesis. In this article, we review the current knowledge regarding the role of the breast tissue microenvironment in regulating the special functions of normal and cancer stem cells. Understanding the crosstalk between MaSCs and their microenvironment will provide new insights into how an altered breast tissue microenvironment could contribute to breast cancer development, progression and therapy response and the implications of this for the development of novel therapeutic strategies to target cancer stem cells.
Contribution to the Study of the Synthesis of Biped Motion with Enhanced Degree of Anthropomorphism

Rapid development of humanoid robots brings about new shifts of the boundaries of Robotics as a scientific and technological discipline. In relation to this, the work raises some new fundamental questions concerning the necessary anthropomorphism of humanoid robots, and how to achieve sufficiently high degree of anthropomorphism with a reasonable number of degrees of freedom. The paper describes a study of the role of hands and twolink trunk in the synthesis of anthropomorphic gait is investigated.
Importance of Virulence Factors for the Persistence of Oral Bacteria in the Inflamed Gingival Crevice and in the Pathogenesis of Periodontal Disease

Periodontitis is a chronic inflammation that develops due to a destructive tissue response to prolonged inflammation and a disturbed homeostasis (dysbiosis) in the interplay between the microorganisms of the dental biofilm and the host. The infectious nature of the microbes associated with periodontitis is unclear, as is the role of specific bacterial species and virulence factors that interfere with the host defense and tissue repair. This review highlights the impact of classical virulence factors, such as exotoxins, endotoxins, fimbriae and capsule, but also aims to emphasize the often-neglected cascade of metabolic products (e.g., those generated by anaerobic and proteolytic metabolism) that are produced by the bacterial phenotypes that survive and thrive in deep, inflamed periodontal pockets. This metabolic activity of the microbes aggravates the inflammatory response from a low-grade physiologic (homeostatic) inflammation (i.e., gingivitis) into more destructive or tissue remodeling processes in periodontitis. That bacteria associated with periodontitis are linked with a number of systemic diseases of importance in clinical medicine is highlighted and exemplified with rheumatoid arthritis, The unclear significance of a number of potential “virulence factors” that contribute to the pathogenicity of specific bacterial species in the complex biofilm–host interaction clinically is discussed in this review.
Biological, nutritional, and therapeutic significance of Moringa oleifera Lam

The genus Moringa Adans. comprises 13 species, of which Moringa oleifera Lam. native to India and cultivated across the world owing to its drought and frost resistance habit is widely used in traditional phytomedicine and as rich source of essential nutrients. Wide spectrum of phytochemical ingredients among leaf, flower, fruit, seed, seed oil, bark, and root depend on cultivar, season, and locality. The scientific studies provide insights on the use of M. oleifera with different aqueous, hydroalcoholic, alcoholic, and other organic solvent preparations of different parts for therapeutic activities, that is, antibiocidal, antitumor, antioxidant, anti‐inflammatory, cardio‐protective, hepato‐protective, neuro‐protective, tissue‐protective, and other biological activities with a high degree of safety. A wide variety of alkaloid and sterol, polyphenols and phenolic acids, fatty acids, flavanoids and flavanol glycosides, glucosinolate and isothiocyanate, terpene, anthocyanins etc. are believed to be responsible for the pragmatic effects. Seeds are used with a view of low‐cost biosorbent and coagulant agent for the removal of metals and microbial contamination from waste water. Thus, the present review explores the use of M. oleifera across disciplines for its prominent bioactive ingredients, nutraceutical, therapeutic uses and deals with agricultural, veterinarian, biosorbent, coagulation, biodiesel, and other industrial properties of this “Miracle Tree. ”
Spatiotemporally Controllable Multiplexed Photothermal Microfluidic Pumping under Monitoring of On-Chip Thermal Imaging.

Intelligent contactless microfluidic pumping strategies have been increasingly desirable in operation of lab-on-a-chip devices. Herein, we present a photothermal microfluidic pumping strategy for on-chip multiplexed cargo transport in a contactless and spatiotemporally controllable fashion, based on the application of near-infrared laser-driven photothermal effect in microfluidic paper-based devices (μPDs). Graphene oxide (GO)-doped thermoresponsive poly (N-isopropylacrylamide)-acrylamide (NIPAM-AcAm) hydrogels served as the photothermally responsive cargo reservoirs on the μPDs. In response to remote contactless irradiation by an 808 nm laser, on-chip phase transition of the composite hydrogels was actuated in a switch-like manner as a result of the photothermal effect of GO, enabling robust on-chip pumping of cargoes from the hydrogels to pre-defined arrays of reaction zones. The thermal imaging technique was employed to monitor the on-chip photothermal pumping process. The microfluidic pumping performance can be spatiotemporally controlled in a quantitative way by remotely tuning the laser power, irradiation time, and GO concentration. The pumping strategy was exemplified by using FeCl3 and horseradish peroxidase (HRP) as the model cargoes to implement on-chip Prussian blue (PB)- and 3,3',5,5'-tetramethylbenzidine (TMB)-based colorimetric reactions, respectively. Furthermore, multiplexed on-demand microfluidic pumping was achieved by flexibly adjusting the irradiation pathway and the microfluidic pattern. The new microfluidic pumping strategy shows great promise for diverse microfluidic applications due to its flexibility, high integratability into lab-on-a-chip devices, and contactless and spatiotemporal controllability.
Total Serum Magnesium Levels and Calcium-To-Magnesium Ratio in Sickle Cell Disease

Background and Objectives: Imbalance of calcium/magnesium ratio could lead to clinical complications in sickle cell disease (SCD). Low levels of magnesium have been associated with sickling, increased polymerization and vaso-occlusion (VOC) in sickle cell due to cell dehydration. The K-Cl cotransport plays a very important role in sickle cell dehydration and is inhibited by significantly increasing levels of magnesium. The study evaluated total serum magnesium levels and computed calcium/magnesium ratio in SCD patients and “healthy” controls. Materials and Methods: The study was a case-control cross-sectional one, involving 120 SCD patients (79 Haemoglobin SS (HbSS)and 41 Haemoglobin SC (HbSC)) at the steady state and 48 “healthy” controls. Sera were prepared from whole blood samples (n = 168) and total magnesium and calcium measured using a Flame Atomic Absorption Spectrometer (Variant 240FS manufactured by VARIAN Australia Pty Ltd., Melbourne, VIC, Australia). Calcium/magnesium ratios were calculated in patients and the controls. Results: The prevalence of hypomagnesemia and hypocalcaemia among the SCD patients was observed to be 39.17% and 52.50% respectively, higher than the controls (4.17% and 22.92%, for hypomagnesemia and hypocalcaemia, respectively). Level of magnesium was significantly lower in the SCD patients compared to their healthy counterparts (p = 0.002). The magnesium level was further reduced in the HbSS patients but not significantly different from the HbSC patients (p = 0.584). calcium/magnesium ratio was significantly higher in the SCD patients (p = 0.031). Although calcium/magnesium ratio was higher in the HbSC patients compared to those with the HbSS genotype, the difference was not significant (p = 0.101). Conclusion: The study shows that magnesium homeostasis are altered in SCD patients, and their levels are lower in HbSS patients. Although calcium/magnesium ratio is significantly higher in SCD patients compared with controls, there is no significant difference between patients with HbSS and HbSC genotypes. Magnesium supplementation may be required in sickle cell patients.
Gender-specific differences in white matter microstructure in healthy adults exposed to mild stress

Abstract Stress is a powerful moderator of brain plasticity and may affect several physiological functions such as the endocrine and the immune system. The impact of stress can be protective or detrimental according to several factors such as level of the stressor and age of occurrence. Also, the impact may differ in males and females. We aim to analyze the effect of mild levels of early and recent stress on white matter microstructure in healthy volunteers. MRI acquisition of diffusion tensor images with a 3.0 T scanner was performed on 130 healthy subjects (71 males and 59 females). Severity of early and recent stress was rated, respectively, on the Risky Families Questionnaire and on the Schedule of Recent Experiences; subjects were divided into low stress and mild stress groups. Mild early stress associated with lower fractional anisotropy (FA) in the cingulate gyrus compared to low early stress. Females reported reduced FA compared to males in the low-stress group in the internal capsule, posterior corona radiata, posterior thalamic radiation, superior longitudinal fasciculus, and sagittal stratum whereas no difference was observed in the mild stress group. An additive effect of early and recent stress was observed in posterior corona radiata, retrolenticular part of the internal capsule, and superior longitudinal fasciculus. The impact of early stress on WM microstructure in healthy subjects is different in males and females. While males seem to be more sensitive to early stress, an additive effect of early and recent stress manifests itself in females. Layman summary Mild levels of early stress associate with lower white matter integrity measured by fractional anisotropy. Females and males show differences in white matter integrity when exposed to low levels of early stress with females showing lower white matter integrity compared to males. No difference in white matter integrity was observed for males and females exposed to mild levels of stress. Mild stress in females is associated with higher white matter integrity. Males seem to be more sensitive to early stress while females are more affected when early stress is followed by stress in adult life.
The Palestinian Elections: Sweeping Victory, Uncertain Mandate

Abstract:The surprise victory of Hamas in the 26 January 2006 Palestinian Authority parliamentary elections has captured worldwide attention and raised major questions about the direction of Palestinian politics and the future of Israeli-Palestinian relations. By giving Hamas a parliamentary majority, Palestinian voters were neither endorsing extremism nor rejecting the peace process. Other Palestinian institutions have the potential to restrain Hamas, but there is a risk that it will turn to Iran or Syria for help. A Hamas-led PA aligned to an Iranian-Syrian axis could lead to either the collapse of the PA or the end of the Palestinian democratic experiment.
Fgf8a mutation affects craniofacial development and skeletal gene expression in zebrafish larvae

ABSTRACT Craniofacial development is tightly regulated and therefore highly vulnerable to disturbance by genetic and environmental factors. Fibroblast growth factors (FGFs) direct migration, proliferation and survival of cranial neural crest cells (CNCCs) forming the human face. In this study, we analyzed bone and cartilage formation in the head of five dpf fgf8ati282 zebrafish larvae and assessed gene expression levels for 11 genes involved in these processes. In addition, in situ hybridization was performed on 8 and 24 hours post fertilization (hpf) larvae (fgf8a, dlx2a, runx2a, col2a1a). A significant size reduction of eight out of nine craniofacial cartilage structures was found in homozygous mutant (6–36%, P<0.01) and heterozygous (7–24%, P<0.01) larvae. Also, nine mineralized structures were not observed in all or part of the homozygous (0–71%, P<0.0001) and heterozygous (33–100%, P<0.0001) larvae. In homozygote mutants, runx2a and sp7 expression was upregulated compared to wild type, presumably to compensate for the reduced bone formation. Decreased col9a1b expression may compromise cartilage formation. Upregulated dlx2a in homozygotes indicates impaired CNCC function. Dlx2a expression was reduced in the first and second stream of CNCCs in homozygous mutants at 24 hpf, as shown by in situ hybridization. This indicates an impairment of CNCC migration and survival by fgf8 mutation. Summary: A function-blocking mutation in fgf8a causes craniofacial malformations in zebrafish larvae due to impaired cranial neural crest cell migration and survival.