Miracle-dz/UniFAR-FTUs huggingface dataset:
Inhibition of tumor cell adhesion , invasion and migration in human lung cancer cells by silencing of TRIM 27

TRIM27 belongs to the family of tripartite motif (TRIM)-containing proteins. Alterative in TRIM proteins have been implicated in several malignancies. The aim of this study was to evaluate the significance of TRIM27 expression in patients with lung cancer and to define its exact role in human lung cancer proliferation, adhesion, invasion and migration. Here, TRIM27 expression was higher in lung cancer tissues more than in adjacent lung tissues. Silencing of TRIM27 using siRNA suppressed proliferation, adhesion, invasion and migration of A549 and H460 cells. Meanwhile, silencing of TRIM27 decreased mRNA and protein expression levels of proliferating cell nuclear antigen (PCNA), cell division cycle 45 (CDC45), matrix metalloprotein 9 (MMP9) and twist family bHLH transcription factor 1 (TWIST1) in A549 and H460 cells. In conclusion, TRIM27 may act as an oncogene through suppressing proliferation and motility in lung cancer carcinogenesis and may serve as potential therapeutic target in lung cancer.
Local Arterial Infusion of Superparamagnetic Iron Oxide Particles in Hepatocellular Carcinoma: A Feasibility and 3.0 T MRI Study

Objectives:We sought to prove feasibility of selective arterial infusion of superparamagnetic iron oxide (SPIO) particles in patients with hepatocellular carcinoma (HCC). Materials and Methods:We studied 13 patients with HCC who underwent modified transarterial chemoembolization (TACE). Six patients received concurrent infusion of Ferucarbotran (Resovist, Schering, Berlin, Germany) in tumor-feeding arteries, and another 6 received MFL AS (MagForce, Nanotechnologies, Berlin, Germany). The iron content of both dispersions was 3.92 mg. One patient served as a control. All patients underwent magnetic resonance imaging (MRI) as baseline and immediate follow-up investigation. Results:Selective arterial infusion of both SPIO particles resulted in significant intratumoral signal intensity decrease on T1-weighted sequences (P < 0.0001), which was greater after MagForce infusion compared with Resovist (P = 0.002). Only minimal amounts of dispersed particles were found in adjacent normal liver parenchyma. No change in intratumoral signal intensity was noted when ferromagnetic particles were omitted. Conclusions:Modified TACE with selective arterial infusion of SPIO particles can be used for precise tumor targeting in patients with HCC, for which MagForce appeared superior to Resovist.
El fenómeno de la polisemia en la lexicografía actual: otra perspectiva

La multifuncionalidad de los signos lingüísticos, el que la misma forma léxica sirva para diferentes usos y reciba diferentes valores semántico-gramaticales, es un fenómeno del lenguaje natural que permite a los sistemas lingüísticos ser muy potentes y algo más económicos. Está ligado a las operaciones de comprensión y producción y apoyado, parece, en la capacidad de conexión múltiple de la mente humana. Fenómenos orales de homofonía (azar/azahar) y convenciones escritas de homografía (real, real y real) contribuyen a complicar este fenómeno: un universal del lenguaje humano (Makkai 1972). La multifuncionalidad se presenta en todos los componentes en que las lenguas pueden analizarse, pero se manifiesta específicamente en el componente léxico. El número tan elevado de unidades léxicas que las lenguas generan explica que este fenómeno sea más evidente en la descripción del léxico que no en la morfología o en la sintaxis. Precisamente uno de los nombres que recibe, el de homonimia, reconoce explícitamente el carácter ‘designativo’, uno de los caracteres propios del léxico. Las manifestaciones extremas del fenómeno se han caracterizado grosso modo como homonimia o como polisemia, según se parta para su descripción de la fonética (en la homonimia dos secuencias coincidentes en la forma fonética no están relacionadas semántico-gramaticalmente) o del significado (en la polisemia se reconocen diferentes valores semántico-gramaticales bajo una misma forma fónica). Homonimia y polisemia son las dos caras extremas del mismo fenómeno, que aquí se enfocará desde la multifuncionalidad, tomando como punto de partida el valor semántico-gramatical de las unidades léxicas, es decir, se enfocará desde la polisemia. La lexicografía está implicada totalmente en este fenómeno lingüístico. Por lo demás no cabe distinguir en él entre lingüística teórica y lingüística aplicada; la descripción y el uso de recursos lingüísticos es una parte importante de la metodología de análisis de la lingüística teórica.
Treatment of Chlamydia trachomatis with a small molecule inhibitor of the Yersinia type III secretion system disrupts progression of the chlamydial developmental cycle

The obligate intracellular bacterium Chlamydia trachomatis possesses a biphasic developmental cycle that is manifested by differentiation of infectious, metabolically inert elementary bodies (EBs) to larger, metabolically active reticulate bodies (RBs). The cycle is completed by asynchronous differentiation of dividing RBs back to a population of dormant EBs that can initiate further rounds of infection upon lysis of the host cell. Chlamydiae express a type III secretion system (T3SS) that is presumably employed to establish and maintain the permissive intracellular niche by secretion of anti‐host proteins. We hypothesize that T3SS activity is essential for chlamydial development and pathogenesis. However, the lack of a genetic system has confounded efforts to establish any role of the T3SS. We therefore employed the small molecule Yersinia T3SS inhibitor N’‐(3,5‐dibromo‐2‐hydroxybenzylidene)‐4‐nitrobenzohydrazide, designated compound 1 (C1), to examine the interdependence of the chlamydial T3SS and development. C1 treatment inhibited C. trachomatis but not T4SS‐expressing Coxiella burnetii development in a dose‐dependent manner. Although chlamydiae remained viable and metabolically active, they failed to divide significantly and RB to EB differentiation was inhibited. These effects occurred in the absence of host cell cytotoxicity and were reversible by washing out C1. We further demonstrate that secretion of T3S substrates is perturbed in C1‐treated chlamydial cultures. We have therefore provided evidence that C1 can inhibit C. trachomatis development and T3SS activity and present a model in which progression of the C. trachomatis developmental cycle requires a fully functional T3SS.
Design of a MgB2 Superconducting Synchronous Generator

A superconducting synchronous generator (SSG) is designed. The magnesium diboride (MgB2) superconducting coils are employed as the field windings. The stator is composed of conventional copper coils and iron core, while the rotor has no iron core. The whole cooling method is adopted in this paper. The temperature of stator area is 70~100 K. The operating temperature of rotor area is 20 K. The thermal barrier is not placed between the stator and the rotor as compared with the prior HTS generator, so the small air gap width would be possible. To study the electromagnetic characteristics of SSG, finite element method (FEM) is implemented to optimize the SSG and obtain the performance of the initial and optimized SSG. The results indicate that the optimized model has better performance.
Parametric rational unsharp masking for image enhancement

Unsharp masking is an effective enhancement tool to improve the visual quality of fine details in images. However, it also amplifies noisy and over-enhances steep edges. To address this problem, this paper proposes a parametric rational unsharp masking. It utilizes the horizontal and vertical gain factors to enhance image details in two directions independently. Experiments and comparisons are provided to demonstrate its excellent enhancement performance.
ENHANCED DEPTH IMAGING OPTICAL COHERENCE TOMOGRAPHY OF THE CHOROID IN CENTRAL SEROUS CHORIORETINOPATHY

Purpose: The purpose of the study was to evaluate the choroidal thickness in patients with central serous chorioretinopathy, a disease attributed to increased choroidal vascular hyperpermeability. Methods: Patients with central serous chorioretinopathy underwent enhanced depth imaging spectral-domain optical coherence tomography, which was obtained by positioning a spectral-domain optical coherence tomography device close enough to the eye to acquire an inverted image. Seven sections, each comprising 100 averaged scans, were obtained within a 5° × 30° rectangle to encompass the macula. The subfoveal choroidal thickness was measured from the outer border of the retinal pigment epithelium to the inner scleral border. Results: The mean age of subjects undergoing enhanced depth imaging spectral-domain optical coherence tomography was 59.3 years (standard deviation, 15.8 years). Seventeen of 19 patients (89.5%) were men, and 12 (63.2%) patients had bilateral clinical disease. The choroidal thickness measured in 28 eligible eyes of the 19 patients was 505 &mgr;m (standard deviation, 124 &mgr;m), which was significantly greater than the choroidal thickness in normal eyes (P ≤ 0.001). Conclusion: Enhanced depth imaging spectral-domain optical coherence tomography demonstrated a very thick choroid in patients with central serous chorioretinopathy. This finding provides additional evidence that central serous chorioretinopathy may be caused by increased hydrostatic pressure in the choroid.
Identification and evaluation of performance parameters for RE-BAR bending training simulator

Construction rebars (steel concrete reinforcing bars) are used to provide structural strength and reinforcement for the concrete structure. This requires the bending and cutting of the rebar to proper size before they can be used for construction. A novel haptic based barbending simulator has been devised which enables the trainees to learn and improve the construction rebar bending skill in a safe and controlled way. With its limited assessment and reporting features, the computerised virtual training simulator proves to be effective in training. Adding the features like personalized skill tracking and predictive performance modeling holds even more potential in supporting the training program. Towards this goal, a user performance modeling needs to be done which includes an initial study on performance parameters, assessment criteria and data collection before remodeling the simulator. This paper presents a study on the performance parameters for the bar bending simulator and also evaluates its effectiveness in modeling expert and novice performances. During this study we also hypothesize the parameters that can distinguish an expert and novice performances which was validated with 2 classification techniques — Support vector machine and J48 Decision tree. While revealing the classification rules J48 algorithm provides 78.94% accuracy where as SVM provides 94.737% accuracy. The study also shows that the 2 performance parameters force applied over time and bend angle accuracy are effective to distinguish expert and novice level of expertize for the construction rebar bending skill.
Simulación Numérica de Transporte de Material Granular por Vibración

Industrias como: química, farmacéutica, semiconductores, agro-alimentaria, minera, metalúrgica, construcción y vidrio hacen uso de material granular en sus procesos y aplicaciones. Las disciplinas científico-técnicas que estudian terremotos, desprendimientos y avalanchas emplean los conocimientos que tenemos de material granular. Azúcar, sal, harina, mineral de hierro, cemento, rocas y arena son ejemplos de productos que nos rodean habitualmente y que están formados por infinidad de partículas sólidas.
Radiation characteristics of zeroth-order resonators composed of 2-D dielectric-based composite right/left handed metamaterial structures

Zeroth-order resonators composed of 2-D dielectric-based composite right/left handed (CRLH) metamaterial structures and their radiation characteristics are investigated. The balanced CRLH structures are designed and fabricated to excite the zeroth-order resonance for the large scale size. The radiation characteristics of the resonators for several configurations with different number of unit cells are numerically simulated and measured. The measurement results are in good agreement with the numerical results, and validate the enhancement of the radiation directivity and gain with the increase in the number of the unit cells.
The Discovery and Development of SU14813, a Next-Generation Multitargeted Tyrosine Kinase Inhibitor for the Treatment of Human Malignancies

Commentary on: [Shem Patyna, A. Douglas Laird, Dirk B. Mendel, Anne-Marie O'Farrell, Chris Liang, Huiping Guan, Tomas Vojkovsky, Stefan Vasile, Xueyan Wang, Jeffrey Chen, Maren Grazzini, Cheng Y. Yang, Joshua O. Haznedar, Juthamas Sukbuntherng, Wei-Zhu Zhong, Julie M. Cherrington, and Dana Hu-
3‐Substituted GABA analogs with central nervous system activity: A review

Gabapentin and Pregabalin are both 3‐alkylated γ‐amino butyric acid (GABA) analogs. Gabapentin was designed as a lipophilic GABA analog and was first synthesized as a potential anticonvulsant and was launched in 1994 as add‐on therapy for the treatment of epilepsy. In this review the discovery and development of gabapentin as an anticonvulsant are discussed. During human trials and while in clinical use, it became apparent that gabapentin induced some other potentially useful therapeutic effects in chronic pain states and behavioral disorders. A review of animal and clinical data relating to these other potential therapeutic utilities is presented. Pregabalin was identified after an investigation into other 3‐substituted GABA analogs. It has since been shown to have a similar pharmacological profile to gabapentin with greater potency in preclinical models of pain and epilepsy. Studies of the mechanism(s) of action of these compounds are discussed. Work towards identifying new analogs of both gabapentin and pregabalin is also reviewed. © 1999 John Wiley & Sons, Inc. Med Res Rev 19, 149–177, 1999.
Tissue‐specific transcriptional enhancers may act in trans on the gene located in the homologous chromosome: the molecular basis of transvection in Drosophila.

The y2 mutation resulted from the insertion of the gypsy element into the X‐linked yellow locus of Drosophila melanogaster. As a consequence of this insertion, transcriptional enhancers that control the expression of the yellow gene in the wings and body cuticle of adult flies are unable to act on the yellow promoter, resulting in a tissue‐specific phenotype characterized by mutant coloration in these structures. Some yellow null alleles (yn) are able to complement the y2 phenotype giving rise to near wild type y2/yn females. The molecular structure of the yellow locus in complementing and noncomplementing mutations was determined by cloning and sequencing the various alleles examined. From the information obtained in these studies, we propose a model suggesting that the complementing wild type phenotype of y2/yn flies might be due to the ability of functional wing and body cuticle transcriptional enhancers located in the yn locus to act in trans on the promoter of the yellow gene found in the y2‐containing chromosome. Furthermore, this transactivation is abolished by the presence of an intact promoter in cis, suggesting that promoter competition between the yellow genes located on each homolog precludes the activation in trans by transcriptional enhancers in favour of cis effects on their own promoter.
Is It Necessary to Repeat Fecal Occult Blood Tests with Borderline Results for Colorectal Cancer Screening?

The fecal immunochemical test (FIT) is the initial non-invasive investigation of choice for population-based colorectal cancer (CRC) screening. We evaluated the positivity rate in repeated tests using the same fecal specimen that showed borderline results in the FIT. A total of 6,465 patients were tested with the FIT in a tertiary-care hospital from July to December 2016. FIT was done using OC-Sensor PLEDIA (Eiken Chemical Co., Tokyo, Japan). Among 6,465 patients, 364 (5.6%) patients showed a positive FIT result of over 20 µg Hb/g feces. A total of 112 (1.7%) patients showed borderline scores of 10.2–20 µg Hb/g feces, and 5,989 (92.6%) patients showed negative results of less than 10 µg Hb/g feces. Among the 101 repeat-tested patients, 19 (18.8%) of the patients' scores converted to levels above the positive cut-off threshold. Repeated results of 19 patients showed score elevations from 20.2 to 68 µg Hb/g feces. These results suggest that it is most important to analyze properly prepared samples, even if only once. Therefore, the laboratory staff should ensure the proper preparation of stool specimens for FIT. Laboratory directors should choose the best cut-off value for detecting CRC at their respective institutions.
HtStuf: High‐Throughput Sequencing to Locate Unknown DNA Junction Fragments

Advances in high‐throughput sequencing have led to many new technologies for assessing genomes and population diversity. In spite of this, inexpensive and technically simple methods for efficiently pinpointing the location of transgenes and other specific sequences in large genomes are lacking. Here we report the development of a modified TA cloning and Illumina sequencing method called high‐throughput sequencing to locate unmapped DNA fragments (HtStuf). Transgenic insertion sites were identified and confirmed in nine out of 10 transgenic soybean [Glycine max (L.) Merr. ] lines, and major rearrangements of the transgene were detected in these lines. Additionally this method was used to map insertions of the introduced DNA transposon, mPing, in four T6 lines derived from a single event. Fifteen of the mPing insertion sites were validated with polymerase chain reaction. Together, these data demonstrate the simplicity and effectiveness of this novel sequencing method.
Soluble and insoluble dietary fibre intake and risk factors for metabolic syndrome and cardiovascular disease in middle-aged adults: the AWHS cohort.

INTRODUCTION The Westernization of the Mediterranean lifestyle has led to a modification of certain dietary habits such as a decrease in the consumption of dietary fibre-rich foods. The impact of these changes on cardiovascular diseases (CVD) has been studied over the last few years and the effect of the different sources of fibre on cardiovascular risk parameters and coronary heart disease (CHD) continues to create controversy. OBJECTIVE To evaluate the association between the source of dietary fibre and the prevalence of metabolic syndrome (MetS) and other cardiovascular risk factors in a Spanish working population. SUBJECTS AND METHODS The study was carried out in a sample of 1592 Spanish workers free of CVD (40-55 years old) within the Aragon Workers' Health Study (AWHS) cohort. Sociodemographic, anthropometric, clinical and biochemical data were collected. Fibre intake was assessed by means of a validated 136-items semiquantitative food-frequency questionnaire. MetS was defined by using the modified National Cholesterol Education Programme - Adult Treatment Panel III (NCEP- ATP III) definition. RESULTS After adjusting for possible confounding factors, we found an inverse association between insoluble fibre intake and systolic and diastolic blood pressure, total cholesterol, triglycerides, apolipoprotein B100 and ratio TG/HDL. Soluble fibre was inversely associated with triglycerides and apolipoprotein B100. Furthermore, prevalence of MetS was found to be lower (OR 0.62, 95% CI: 0.40-0.96) in those participants in the highest quartile of insoluble fibre intake. CONCLUSION A higher intake of insoluble fibre could play an important role in the control and management of hypertension, lipid profile and MetS.
Associations between substance use disorders and major depression in parents and late adolescent-emerging adult offspring: an adoption study.

AIMS To examine whether major depressive disorder (MDD) and substance use disorders [SUDs: specifically, nicotine dependence (ND), alcohol use disorders (AUDs), and cannabis use disorders (CUDs)] in parents predicted increased risk for these disorders in late adolescent-emerging adult offspring and, specifically, the extent to which the pattern of risk differed for adopted and non-adopted youth. PARTICIPANTS Late adolescent and emerging adult participants from the Sibling Interaction and Behavior Study (mean age = 18.8), a community-based investigation of adopted and non-adopted adolescents, and their parents (adoptive parents of adopted youth, biological parents of non-adopted adolescents) were included. MEASUREMENTS Structured interviews were used to assess these disorders. FINDINGS (i) When the same disorder in parents and adolescents was examined, parental MDD was associated with increased risk for MDD among both adopted (P < 0.001) and non-adopted (P < 0.01) adolescents; in contrast, SUDs were associated with increased risk for the same SUD in non-adopted offspring (all P < 0.01). (ii) When cross-SUD effects were examined, for the most part, each SUD was associated with increased risk for other SUDs among non-adopted but not adopted offspring (most P < 0.05). (iii) When MDD-SUD associations were examined, parental ND and CUDs predicted increased risk for MDD in non-adopted (P < 0.001), but not adopted, adolescents. These effects tended to remain significant when adjusting for within-person comorbidity (P < 0.05). CONCLUSIONS Major depressive disorder in parents appears to be a risk factor for late adolescent-emerging adult major depressive disorder but not substance use disorder in offspring, with this risk being environmentally mediated. Substance use disorder in parents appears, via genetic mediation, to increase risk of substance use disorder in adolescent offspring, and cannabis and nicotine use disorders in parents contribute similarly to major depressive disorder in those offspring.
Exercise, bone and nutrition

Predisposition to poor skeletal health resulting in osteoporotic fracture is a major public health problem, the future economical impact of which is likely to be phenomenal. Two mechanisms principally determine adult bone health: (1) maximum attainment of peak bone mass (PBM); (2) the rate of bone loss with advancing age. Both aspects are regulated by a combination of endogenous and exogenous factors, and although genetic influences are believed to account for up to 75 % of the variation in bone mass, there is still room for modifiable factors to play a vital role. Weight-bearing physical activity is beneficial to the skeleton, but clarification of the exact type, intensity and duration required for optimum bone mass is needed. Excessive levels of exercise, which result in amenorrhoea, are detrimental to skeletal health. The importance of Ca to bone remains controversial. There is evidence that Ca is effective in reducing late post-menopausal bone loss, but more research is required on the long-term benefit of increased Ca intake on PBM attainment. Vitamin D ‘insufficiency’ appears to be widespread amongst population groups and is an area of considerable public health concern. The role of other micronutrients on bone metabolism remains to be fully quantified, but data from a combination of experimental, clinical and observational studies suggest a positive link between alkaline-forming foods and indices of bone health. The influence of nutrient–gene interactions on the skeleton requires further elucidation, but it may be useful in the future to target nutrition advice at those individuals who are genetically susceptible to osteoporosis.