Miracle-dz/UniFAR-FTUs huggingface dataset:
Protein sequencing by matrix-assisted laser desorption ionization-postsource decay-mass spectrometry analysis of the N-Tris(2,4,6-trimethoxyphenyl)phosphine-acetylated tryptic digests.

We have recently reported a simple procedure by which low picomole quantities of peptides can be modified to the corresponding N-Tris(2, 4,6-trimethoxyphenyl)phosphonium-acetyl (TMPP-Ac) derivatives (Z. H Huang, J. Wu, D. A. Gage, and J. T. Watson, Anal. Chem. 69, 137-144, 1997). This modification significantly facilitates sequence interpretation by providing exclusively N-terminal product ions (mainly a-type ions) in the fast-atom bombardment-MS/MS and matrix-assisted laser desorption ionization-postsource decay(MALDI-PSD)-MS spectra. The TMPP-Ac derivatization approach has been extended now for the direct derivatization of tryptic digests originating from 1-5 microg of proteins with molecular weights from 10-120 kDa. Our new procedure involves tryptic digestion in aqueous solution buffered to pH 8-8.2 with phosphate or Tris-HCl, followed by reaction with TMPP-acetic acid N-hydroxysuccinimide ester (TMPP-AcOSu bromide, 2-4 nmol reagent/microg protein, rt, 20 min) to provide N-terminally derivatized products, while the epsilon-NH2 groups in lysine remain unchanged. The resultant derivatized peptide mixture or its partially separated HPLC fractions are subsequently analyzed by MALDI-PSD-MS using 0.5- to 1-pmol aliquots, giving rise to product ion spectra that are easily interpretable. As there is no need for material transfer and change of buffer media, the tandem enzymatic-chemical reaction/MS analysis process is usually carried out with very high throughput (digestion, 1 h; reaction, 1/3 h; HPLC, 1 h; MALDI-PSD, 3-4 fragments/h). This procedure will be of potential use for obtaining sequence information directly from mixtures or as an adjunct of peptide mass mapping to provide protein identification with high confidence.
DMD pseudoexon mutations: splicing efficiency, phenotype, and potential therapy

The degenerative muscle diseases Duchenne (DMD) and Becker muscular dystrophy result from mutations in the DMD gene, which encodes the dystrophin protein. Recent improvements in mutational analysis techniques have resulted in the increasing identification of deep intronic point mutations, which alter splicing such that intronic sequences are included in the messenger RNA as “pseudoexons. ” We sought to test the hypothesis that the clinical phenotype correlates with splicing efficiency of these mutations, and to test the feasibility of antisense oligonucleotide (AON)–mediated pseudoexon skipping.
A review on urolithiasis in dogs and cats.

Urolithiasis is a nutritional disease that affects domestic carnivores. Past and recent literature on urolithiasis was reviewed for information on anatomical occurrence, physiology of urine formation, prevalence, mineral composition, clinical signs, laboratory findings, dissolution therapy, surgery and prevention of urolithiasis. The acquired knowledge of complexed and multifaceted urolithiasis is a tremendous achievement towards the treatment and control of the disease. However, eradication of the disease is the most challenging as it requires total overhaul of all the factors that are responsible for the formation of uroliths.
Utility of Optical Coherence Tomography Imaging with Angiographic Co-registration for the Guidance of Percutaneous Coronary Intervention

© R A D C L I F F E C A R D I O L O G Y 2 0 1 4 Intracoronary optical coherence tomography (OCT) is a light-based imaging modality able to visualise with high resolution (~10 μm) the vascular morphology and the acute and chronic effects of intervention with intracoronary devices. OCT could therefore find application in the guidance of percutaneous coronary intervention (PCI), allowing a thorough preprocedural lesion assessment, which enables accurate device sizing, selection of the vessel segment requiring treatment, and, thus, efficient planning of the implantation strategy (see Table 1). Moreover, it can be used for the assessment of the acute procedural result, allowing the estimation of stent expansion and vessel injury. Consequently, intravascular imaging can in this way assist in the optimisation of the acute implantation result, the significance of which is underscored by observations of an association between suboptimal implantation and stent failure. Importantly, several studies and meta-analyses have shown that the use of imaging guidance might improve outcome.
v-t Characteristics using extended disruptive effect model for impulses of varying front times

Overvoltages due to internal and external sources stress the insulation of the power system equipment. Usually the insulation design of any power equipment is tested with standard lightning impulse voltage. In practical cases, the insulations are stressed with overvoltages of non-standard waveshapes like steep fronted, Very Fast Transient Overvoltages (VFTO) and oscillatory impulses also. This demands the study on behavior of insulating materials under non-standard impulses. The prediction of v-t characteristics of any insulating material for such non-standard overvoltages is required for proper insulation design. In this regard, an attempt is made to characterize air insulation using existing Disruptive Effect model for standard lightning impulse (1.2/50 μs) and is found successful only for time to breakdown (tb) greater than 2 μs. Hence, an extended Disruptive Effect model is proposed to cater to tb less than 2μs and the model is verified up to VFTO. The model is also verified for impulses of varying front times between 90 ns to 1.2 μs with oscillations.
Intelligent Models to Identification and Treatment of Outliers in Electrical Load Data

The Electrical Load data are stored in each time interval generating big databases with high dimensional data. Each data stored contains significant information that can assist the planning and operation of electrical system. In the data analysis step, many aspects must be considered such as the data consistency and the identification and treatment of outliers. This is a critical step because data quality is directly reflected in the results of the planning and operation of electrical system. This paper proposes two models for the identification and treatment of outliers in electrical load data. The first model was built using the ensemble technique through a combination of individual models. The second model was created from an expert system that uses a rules database to detect outliers. The processing of the outliers detected is conducted through a combination of non-outliers load in the same time interval. To evaluate the performance, the models were applied in a historical load database measured in the Northeast of Brazil during year of 2006. The results showed that the proposed models showed satisfactory results in terms of detection as well in the treatment of outliers.
68Ga-PSMA PET/CT Detects the Location and Extent of Primary Prostate Cancer

We evaluated the accuracy of PET/CT with 68Ga-PSMA-HBED-CC—a 68Ga-conjugated ligand of human prostate-specific membrane antigen (PSMA)—to localize cancer in the prostate and surrounding tissue at initial diagnosis. Methods: Twenty-one patients with biopsy-proven prostate cancer underwent 68Ga-PSMA-HBED-CC (68Ga-PSMA) PET/CT at a median of 4 d (range, 0–47 d) before radical prostatectomy. Based on a 6-segment model, the Gleason score and proportion of tumor tissue within each segment (segmental tumor burden, or STB) as determined by histopathology (STBHP) were correlated with SUVmax and STB as determined by different SUV cutoffs for 68Ga-PSMA PET (STBPET1–6). Furthermore, the involvement of seminal vesicles and other extracapsular extension were assessed by histopathology and PET/CT. Results: Histopathology-positive segments (n = 100 of 126; 79%) demonstrated a significantly higher mean ± SD SUVmax (11.8 ± 7.6) than histopathology-negative segments (4.9 ± 2.9; P < 0.001). Receiver-operating-characteristic analysis revealed an optimal SUVmax cutoff of 6.5 for discrimination of histopathology-positive segments from histopathology-negative segments (area under the curve, 0.84; P < 0.001), which gave 67% sensitivity, 92% specificity, a 97% positive predictive value, a 42% negative predictive value, and 72% accuracy. STBPET3 as determined by (2 × blood SUV) + (2 × SD) correlated best with STBHP (Pearson ρ = 0.68; P < 0.001; mean difference ± SD, 19% ± 15%). PET/CT correctly detected invasion of seminal vesicles (n = 11 of 21 patients; 52%) with 86% accuracy and tumor spread through the capsule (n = 12; 57%) with 71% accuracy. Conclusion: 68Ga-PSMA PET/CT accurately detected the location and extent of primary prostate cancer. Our preliminary findings warrant further investigation of 68Ga-PSMA PET/CT in conjunction with needle biopsy.
Affine models for motion and shape recovery

This paper presents an affine model for 3-D motion and shape recovery using two perspective views and their relative 2-D displacement field. The 2-D displacement vectors are estimated as parameters of a 2-D affine model that generalizes standard block matching by allowing affine shape deformations of image blocks and affine intensity transformations. The matching block size is effectively found via morphological size histograms. The parameters of the 3-D affine model are estimated using a least-squares algorithm that requires solving a system of linear equations with rank three. Some stabilization of the recovered motion parameters under noise is achieved through a simple form of MAP estimation. A multi-scale searching in the parameter space is also used to improve accuracy without high computational cost. Experiments on applying these affine models to various real world image sequences demonstrate that they can estimate dense displacement fields and recover motion parameters and object shape with relatively small errors.
Assessment of Inflammasome Formation by Flow Cytometry

Inflammasomes are large protein complexes formed in response to cellular stresses that are platforms for recruitment and activation of caspase 1. Central to most inflammasome functions is the adapter molecule ASC (apoptosis‐associated speck‐like protein containing a caspase‐recruitment domain) that links the inflammasome initiator protein to the recruited caspases. ASC is normally diffuse within the cell but within minutes of inflammasome activation relocates to a dense speck in the cytosol. The dramatic redistribution of ASC can be monitored by flow cytometry using parameters of fluorescence peak height and width when immunostained or tagged with a fluorescent protein. This can be used to define cells with active inflammasomes within populations of primary macrophages and monocytes, allowing quantification of responses and flow‐sorting of responding cells. Protein structural requirements for ASC speck formation and recruitment of caspases to ASC specks can be assessed by expressing components in HEK293 cells. This provides rapid quantification of responding cell number and correlation with the expression level of inflammasome components within single cells. © 2016 by John Wiley & Sons, Inc.
A technique for the perfusion of laboratory animals for the recovery of schistosomes.

The search for and the recovery of schistosomes from laboratory animals presents a problem if the total number of worms in the animal must be ascertained, or if comparative figures must be compiled on the number of worms present in the blood vessels of various organs and regions. The most important area involved is the mesenteric-portal system, but worms may also be present, especially during their migration, in the pulmonary and systemic circulations. Inspection of the mesenteric veins and main portal vein, and manual dissection or comminution of the liver are not likely to reveal all of the worms, especially the small ones, and the time consumed in removing them individually is considerable. A search for worms early in the infection would be impossible by this technique. A desirable method is one which will standardize the individual differences of the operators, effect a reliable removal of worms, and be applicable to early infections. A modification of the perfusion technique of Faust and Meleney (1924) serves these purposes. The principle of this technique is to isolate the organs and regions to be examined and to perfuse the vessels in which the worms may be present from their smallest to their largest branches. They accomplished this in the case of the mesenteric system and the extremities by perfusing from artery to vein, in the case of the liver by perfusing from hepatic vein to the portal vein, and in the case of the lungs by perfusing from artery to vein. Improvements of the technique, made in this laboratory, include supplying a continuous flow of the perfusing fluid, arranging the apparatus in such a way that several animals may be perfused simultaneously, and adding details to obtain more complete collection of worms.
Design of a Dual W- and D-Band PLL

This paper describes the design considerations and performance of the highest frequency phase-locked loop (PLL) reported to date. The PLL was fabricated in a 0.13-μm SiGe BiCMOS process and integrates on a single die: a fundamental-frequency 86-92 GHz Colpitts voltage-controlled oscillator (VCO), a differential push-push 160-GHz Colpitts VCO with two differential outputs at 80 GHz, a programmable divider chain, the charge pump, and all loop filter components. It achieves the lowest W- and D-band phase noise of -93 dBc/Hz at 90 GHz and -87.5 dBc/Hz at 163 GHz, both measured at a 100 kHz offset, and demonstrates an extended locking range of 80-100 GHz at the fundamental frequency, and 160-169 GHz at the second harmonic output of the push-push VCO. The single-ended PLL output power is -3 dBm at 90 GHz and -25 dBm at 164 GHz. The chip consumes 1.25 W from 1.8 V, 2.5 V, and 3.3 V supplies and occupies 1.1 mm × 1.7 mm, including pads.
Bipartite Digraphs Debates

A novel graph-based model for aggregating dichotomous preferences is introduced. The output opinion is viewed as a consensual situation, paving the way of using graph operations to describe properties of the aggregators. The outputs are also dichotomous preferences which could be useful in some applications. New axiomatic characterizations of aggregators corresponding to usual majority or approval &disapproval rule are presented. Integrating and exploiting Dung’s Argumentation Frameworks and their semantics into our model is another contribution of the present paper.
Reduction of air pollution potential through environmental planning.

Amelioration of the air pollution problem, whether the result of mobile or stationary source emissions, will depend primarily upon mechanical controls and fuel and process changes. Urban and transportation planning, however, may provide supplementary means for reducing both emissions of pollutants and the exposure of persons to undesirably high ambient concentrations. The body of information in this paper is directed to the planners and policy-makers whose decisions influence the nature of our environment. Three general areas are identified in which the policies, plans, and standards developed by environmental planners influence the air pollution problem : 1. Long-term, large-scale urban planning involving entire or substantial parts of metropolitan areas. 2. Design and operation of transportation systems. 3. Location, design, and construction of individual roadways and structures. In each of these three areas, the Air Pollution Control Office has initiated projects that will assist planners in evaluating.
Latent homologues for the neural crest as an evolutionary novelty

The neural crest is a craniate synapomorphy and a bona fide evolutionary novelty. Recently, researchers considering intriguingly similar patterns of gene expression, cell behaviors, and embryogenetic processes in noncraniate deuterostomes have suggested that cephalochordates, urochordates, and echinoderms or their ancestors might have possessed cells that were precursors to the neural crest or its constituent cells. To emphasize the caution with which similarities at genetic, cellular, or embryological levels should be interpreted as substantiations for cell, germ layer, or tissue homologies, we present and evaluate additional tantalizing evidence that could be considered as documenting neural crest precursors in precraniates. Furthermore, we propose an evolutionary context—latent homologue—within which these data should be interpreted.
Aspectos físicos e socioeconômicos da bacia hidrográfica do Rio Ivinhema

O conteudo e apresentado em sete capitulos: 1 - Resumo; 2 - Introducao; 3 - Aspectos fisicos; 4 - Aspectos socioeconomicos; 5 - Aptidao agricolas das terras; 6 - Consideracoes finais; 7 - Referencias bibliograficas.
Metabolites of mitragynine.

Microbial transformation of the alkaloid mitragynine by the fungus Helminthosporum sp. has elaborated two major metabolites. The compounds were isolated from the biological milieu and their structures were elucidated as mitragynine pseudoindoxyl and hydroxy mitragynine pseudoindoxyl. Tests to date have indicated quite high pharmacological activity by mitragynine pseudoindoxyl. Both metabolites were synthesized to verify structures.
Inflammation, neovascularization and intra-plaque hemorrhage are associated with increased reparative collagen content: Implication for plaque progression in diabetic atherosclerosis

Sustained inflammation may stimulate a reparative process increasing early reparative type III collagen synthesis, promoting atherosclerotic plaque progression. We evaluated inflammation, neovascularization, intra-plaque hemorrhage (IPH), and collagen deposition in human aortic atherosclerotic plaques from patients with and without diabetes mellitus (DM). Plaques were procured at autopsy from lower thoracic and abdominal aorta from DM (n = 20) and non-DM (n = 22) patients. Inflammation and neovascularization were quantified by double-label immunochemistry and the IPH grade was scored using H&E-stained sections. Type I and type III collagens were quantified using Picro-Sirius red stain with polarization microscopy and computerized planimetry. In non-DM plaques, 27%, 40%, and 33% had mild, moderate and severe inflammation in the fibrous cap and shoulder compared with 2%, 30% and 68% in DM plaques (p < 0.001). The geometric mean neovessel count was increased in DM versus non-DM plaques (140 [95% CI: 119—165] versus 59 [95% CI: 51—70]; p < 0.001). The IPH grade was increased in DM verses non-DM plaques (0.82 ± 0.11 versus 0.29 ± 0.11; p < 0.001) (percentage grade). The density of type III was increased in DM plaques (0.16 ± 0.01 versus 0.06 ± 0.01; p < 0.001) with a non-significant reduction in type I density in DM when compared with non-DM (0.28 ± 0.03 versus 0.33 ± 0.03; p = 0.303) (content per mm2). The increase in type III collagen content correlated with total neovessel content (r = 0.58; p < 0.001) in DM plaques. In conclusion, our study suggests that enhanced type III collagen deposition was associated with inflammation, neovascularization and IPH, and may be a contributing factor in DM plaque progression.
Performance analysis of communication protocols for Internet of things platforms

The Internet of Things (loT) allows transform current Internet communication in an automatically way through Machine-to-Machine (M2M) communication. The IoT probably has become one of the most popular networking concepts that has the potential to bring out many benefits. However, IoT has brought some communications challenges. In the next three years, it is expected that more than 20 billion IoT devices to be connected, thus, communication protocols must allow massive multicasting, low overhead, and simplicity to operate on small platforms. This paper presents a comparative study of the main communication protocols that allows the implementation of an IoT platform, to determine their computational load, overhead and network bandwidth. Communication protocols such as MQTT, AMQP, CoAP and XMPP were tested to determine CPU processing, transferred bytes and sent packets for different communication scenarios.
An investigation into the human serum “interactome”

The protein content of human serum is composed of a millieu of proteins from almost every type of cell and tissue within the body. The serum proteome has been shown to contain information that directly reflects pathophysiological states and represents an invaluable source of diagnostic information for a variety of different diseases. Unfortunately, the dynamic range of protein abundance, ranging from ≫ mg/mL level to ≪ pg/mL level, renders complete characterization of this proteome nearly impossible with current analytical methods. To study low‐abundance proteins, which have potential value for clinical diagnosis, the high‐abundant species, such as immunoglobulins and albumin, are generally eliminated as the first step in many analytical protocols. This step, however, is hypothesized to concomitantly remove proteins/peptides associated with the high‐abundant proteins targeted for depletion. In this study, immunoprecipitation was combined with microcapillary reversed‐phase liquid chromatography (μRPLC) coupled on‐line with tandem mass spectrometry (MS/MS) to investigate the low‐molecular‐weight proteins/peptides that associate with the most abundant species in serum. By this targeted isolation of select highly abundant serum proteins, the associated proteins/peptides can be enriched and effectively identified by μRPLC‐MS/MS. Among the 210 proteins identified, 73% and 67% were not found in previous studies of the low‐molecular‐weight or whole‐serum proteome, respectively.
Meat Science and Applications

Meat science - chemistry, biochemistry and biotechnology: meat composition postmortem muscle chemistry meat colour flavours of meat products analytical methods meat biotechnology. Meat safety: microbiology of meats meat safety drug residues in meat - emerging issues. Slaughtering and carcass processing: antemortem handling and welfare slaughtering and processing equipment carcass processing -factors affecting quality carcass processing - quality controls electrical inputs and meat processing. Processing meats: meat and meat products spices and flavourings for meat and meat products intermediate-moisture meat and dehydrated meat manufacturing of reduced-fat, low-fat and fat-free emulsion sausage meat packaging - protection, preservation and presentation meat curing technology meat smoking technology meat canning technology meat fermentation technology. Meat production, byproducts, workers' safety and waste management: meat production meat co-products occupational safety waste management.
Psychology of Computer Use: XLVI. Age-Related Differences in the Mapping of Auditory Icons to Visual Icons in Computer Interfaces for Children

An investigation was conducted to characterize how children ages 6 through 9 identify auditory icons present in educational software. 24 subjects were required to map auditory icons to visual icons, both present in a computer interface. The interface used in the experiment was constructed with Visual Basic and involved 40 auditory icons, 40 corresponding visual icons, and 66 extraneous visual icons. It was hypothesized that older children would be better able to map the auditory icons to visual icons due to more extensive exposure to everyday sounds. The results supported the hypothesis and suggestions for additional research were provided.
Synthesis and Analysis of Nucleosides Bearing Pyrrolepolyamide Binding to DNA

Abstract An efficient synthesis of adenosine bearing pyrrolepolyamide 1 was achieved by coupling of 3 with 2. The CD spectra obtained at several [ligand ]/[duplex] ratios allowed verification of the formation complex of the DNA duplex [d(CGCAAATTGGC)/d(GCCAATTTGCG)] with 1.