Miracle-dz/UniFAR-FTUs huggingface dataset:
Preparasi sel mamalia CHO-DG44 dan isolasi plasmid dari bakteri Eschericia coli DH5-alfa sebagai tahap dalam produksi protein terapeutik erythropoetin sebagai obat anemia

Erythropoietin is a hormone that stimulates the bone marrow to produce red blood cells needed by the human body to carry oxygen to vital organs. CHO-DG44 cells are one of the mammalian cells that can be used for the production of erythropoietin. This study aims was to determine the optimum growth of CHODG44 cells for 12 days using density and viability parameters and determine the characteristics of E. coli DH5 alpha bacterial DNA isolates using electrophoresis method which is suitable for transfection into CHO-DG44 cells. The results showed that CHO-DG44 cells had an average viability of 69.82% for media 1 and 75.99% for media 2 with a mean density of 3.130,000 cells/mL and 4,106,000 cells/mL respectively. While the culture media that produced cells with high viability were on day 4 for erlenmeyer 1 and day 6 for erlenmeyer 2. From the results of electrophoresis, plasmid DNA had a size of 5954 bp with a DNA JURNAL BIOLOGI UDAYANA 24(2): 87-95 P ISSN: 1410-5292 E ISSN: 2599-2856 88 concentration of 117 ng/μl, which according to the size and concentration of plasmid DNA so that it can be transfected into CHO-DG44 cells.
Gender Implications of Terrorism and Criminality in the North-East Nigeria- 2013- 2018, Borno State, Nigeria

in her own territory. The occurrence of violence such as that perpetrated by Boko Haram, on female students, the issues of kidnapping and banditry, the rampant cases of baby, child and adult rape of both female or male clan, and the illicit acts of human trafficking and drug abuse presents yet another hurdle hindering Nigeria’s progress. Nigerian security forces in dealings with terrorists and criminals like Boko Haram terrorists and other illicit groups have generated international concerns. Consequently, foreign nations are unwilling to lend a helping hand in sales of arms, rescue operations and humanitarian aids thereby worsening the humanitarian crises affecting mostly women and Abstract: The female gender is indeed an endangered species cut up in the manifestation of terrorist attacks, and other forms of violence and criminality. Nigeria recently experienced some levels of terrorism. The state of terrorism engendered by Boko Haram terrorist in Nigeria, especially in the North-Eastern part of the country, is quite worrisome, disheartening and alarming. Terrorist attacks of the Boko Haram sect have resulted in the killing of countless number of innocent people and wanton destruction of properties worth billions of naira through bombings, kidnapping and banditry. The study ascertains the impact of terrorism and criminality specifically on the female gender, caused by Boko Haram in Nigeria generally, using Mongocolis and Bakassi IDP camps in the north-eastern Nigeria Borno state as a case study. The research used both primary and secondary sources to collect data. Economic Deprivation theory was used to analyze the gender implication of terrorism and criminality. The study concluded that criminality and Boko Haram activities in North-Eastern Nigeria has crippled social economic activities, caused psychological harm to women, children as well as caused security imbalance. The research recommends an overhaul in the security approach to terrorism and criminalities in the country, backed up with extant laws and punitive measures to serve as deterrent to offenders. Also, government should provide sufficient skill, manpower, equipment and funding to the military and security agencies for the discharge of their job. Nigerian government should intervene quickly and provide adequate support to the victims, military and civilians where necessary.
Characterization of Secondary Structure of Pig Hair Fiber Using Fourier-transform Infrared Spectroscopy

ABSTRACT Hair or bristle is one of the key by-products of humane slaughter of pigs. In the present study, Fourier-transform infrared (FTIR) spectroscopy was used for examining protein secondary structure of hair fiber from different breeds of pigs (Ghungroo, Ninag Megha, Hampshire, and Duroc). Prominent amide I (1635–1648 cm−1) and amide II (1517–1528 cm −1) bands could be observed in the spectra. Differences in presence/absence of certain FTIR bands between breeds suggests influence of genetic background in the structure of hair fiber. Deconvolution resolved secondary structure of protein in amide I region of spectra into corresponding α-helix (38.0 ± 2.9%), β-sheets (32.2 ± 2.0%), β-turns (19.4 ± 3.1%) and unorganized structural components (10.3 ± 2.5%). Heating of fibers from 80°C to 120°C resulted in changes in amide regions and α-helix to β-sheet ratio of amide I band. Further, correlations were calculated between area under the curve of various FTIR bands and tensile properties of the fiber. Area of FTIR band at 1635 cm −1 was positively correlated with tenacity, initial modulus, extensibility, and work of rupture (r = 0.30 to 0.39). In nutshell, the study reveals subtle differences in secondary structure of hair with respect to breed or temperature treatment and suggests relation between FTIR spectral characteristics and tensile properties.
Modified Multi-Crossover Operator NSGA-III for Solving Low Carbon Flexible Job Shop Scheduling Problem

Low carbon manufacturing has received increasingly more attention in the context of global warming. The flexible job shop scheduling problem (FJSP) widely exists in various manufacturing processes. Researchers have always emphasized manufacturing efficiency and economic benefits while ignoring environmental impacts. In this paper, considering carbon emissions, a multi-objective flexible job shop scheduling problem (MO-FJSP) mathematical model with minimum completion time, carbon emission, and machine load is established. To solve this problem, we study six variants of the non-dominated sorting genetic algorithm-III (NSGA-III). We find that some variants have better search capability in the MO-FJSP decision space. When the solution set is close to the Pareto frontier, the development ability of the NSGA-III variant in the decision space shows a difference. According to the research, we combine Pareto dominance with indicator-based thought. By utilizing three existing crossover operators, a modified NSGA-III (co-evolutionary NSGA-III (NSGA-III-COE) incorporated with the multi-group co-evolution and the natural selection is proposed. By comparing with three NSGA-III variants and five multi-objective evolutionary algorithms (MOEAs) on 27 well-known FJSP benchmark instances, it is found that the NSGA-III-COE greatly improves the speed of convergence and the ability to jump out of local optimum while maintaining the diversity of the population. From the experimental results, it can be concluded that the NSGA-III-COE has significant advantages in solving the low carbon MO-FJSP.
AMBIENTE DE ENSINO-APRENDIZAGEM NA FORMAÇÃO INICIAL UNIVERSITÁRIA DE EDUCAÇÃO FÍSICA

Objetivou-se analisar as percepções de estudantes universitários de Educação Física em distintos níveis de progressão no curso, sobre o ambiente de ensino-aprendizagem na formação inicial. Participaram 273 estudantes (Bacharelado n=150; Licenciatura n=123) do curso de licenciatura em Educação Física de uma universidade pública de Santa Catarina, Brasil, os quais responderam à versão adaptada do Questionário de Avaliação do Ambiente Percebido da Formação Inicial em Educação Física. O teste Qui-quadrado foi utilizado para analisar as associações entre o nível de progressão discente nos cursos e suas percepções sobre o ambiente de formação inicial. Os resultados indicaram a predominância de aulas/vivências práticas e de avaliações teóricas escritas, especialmente nas fases iniciais. As experiências poucos frequentes de observação, e o aprendizado pela prática ou por observação foi o papel assumido, especialmente evidenciados nos semestres finais. Apesar de os estudantes terem se percebido ativos quanto ao seu nível de participação, indicaram que os professores são os principais responsáveis por tomar as decisões. Conclui-se que a formação inicial em Educação Física necessita reconfigurar determinadas práticas de ensino-aprendizagem para aumentar o envolvimento e a responsabilidade discentes pelo próprio processo formativo.
EFEKTIVITAS PERLINDUNGAN MASKER GEL PEEL OFF EKSTRAK ETANOL 96% KULIT BUAH MANGGIS (Garcinia mangostana L.) DALAM MEMPERTAHANKAN PH KULIT TIKUS WISTAR JANTAN YANG DIBERI PAPARAN SINAR UV-B

Radiasi sinar UV-B memiliki kapasitas untuk menghasilkan reactive oxygen species (ROS) yang menyebabkan terjadinya photoaging yang dapat diamati dari nilai pH kulit. Ekstrak kulit buah manggis diketahui memiliki kandungan antioksidan yang tinggi dan telah diformulasi menjadi sediaan masker gel peel off . Sediaan ini diketahui mampu meningkatkan toleransi kulit tikus terhadap paparan sinar UV-B. Pada penelitian ini dilakukan pengujian untuk mengetahui efektivitas masker gel peel off ekstrak kulit buah manggis dalam menjaga pH kulit tikus wistar jantan yang diberi paparan sinar UV-B. Penelitian dilakukan dengan menggunakan 30 hewan uji yang dibagi dalam 3 kelompok, yaitu perlakuan I (basis masker gel peel off ), perlakuan II (masker gel peel off ekstrak etanol 96% kulit buah manggis) dan kontrol (tanpa perlakuan). Kelompok perlakuan I dan perlakuan II dipapar dengan UV-B dengan dosis total 840 mJ/cm² selama 28 hari. Hasil penelitian menunjukkan bahwa kelompok perlakuan II berbeda bermakna dengan kelompok perlakuan I dan kelompok kontrol dengan nilai signifikansi P<0,05. Sehingga diketahui masker gel peel off ekstrak kulit buah manggis mampu mempertahankan pH kulit tikus wistar jantan yang dipapar sinar UV-B.
Transcriptome Signatures of Dysregulated Brain Dynamics Induce Entangled Network States.

Marks of brain disorders may be visible through abnormal networks characterized by states with distinct signatures or connectivity patterns. Apart from neurodegenerations, drug abuse or eventually addiction may exert complex perturbative effects over human health, including damage to various organs and especially brain inflammation, volume shrinkage and functional deficits. These can also be regarded as signs of accelerated ageing, which involve multiple comorbidities associated with long-lasting cognitive impairments. The present study investigates drug effects at brain level through transcriptional changes potentially reflecting specific molecular and cellular dysregulation marks. As signatures arising in various brain regions may change, the data here analyzed refer to cross-referenced transcriptome profiles that were in part experimentally measured and in part computed. A methodological aim refers to network inference centered on a few well-characterized entangled states whose connectivity patterns identify clusters and motifs. At the biological annotation level an interesting role is played by the Vascular Endothelial Growth Factor A (VEGFA), a central connector of kinases involved in cytoskeleton rearrangement, synaptogenesis and synaptic functions, neurogenesis, cell growth, cell adhesion, neurohormone signaling apoptosis. VEGFA associations in both co-expression and protein-protein interaction networks reveal patterns linked to MCL1, a BCL2 family Apoptosis regulator.
Expulsion: A type of forced mobility experienced by homeless people in Canada

ABSTRACT In the last 30 years, researchers have increasingly examined (im)mobility to understand why homelessness continues in the Global North. Studies consistently identify how people experiencing homelessness are excluded and displaced. However, the relationship between social and spatial marginalization is not well understood. In this article, I argue that a more complete understanding of mobility requires joint attention to social exclusion and spatial displacement. I develop the concept of expulsion to examine homeless mobilities. I draw on data from a longitudinal survey of homeless people’s inter-regional mobilities over ten years in Canada. I identify six modes of expulsion: residential expulsions, service exclusions, warehousing, racial banishments, violent expulsions, and ostracism. In each of these instances, people are mobilized to the social exterior while they are simultaneously displaced spatially. By developing a theory of expulsion, this paper contributes a new framework for understanding forced migration, coerced mobility, and involuntary displacement.
RELATIONSHIPS BETWEEN FRUGIVOROUS- BIRDS AND THEIR HABITATS IN FRAGMENTED FOREST AREAS NEAR TO VILLAVICENCIO CITY, VILLAVICENCIO, META, COLOMBIA.

The degradation of the Llanos foothill ecosystems is one of the biggest ecological problems in the areas surrounding the city of Villavicencio, Meta. The generation of fragments produces new conditions for the establishment of bird guilds. This study was carried out during 2017 - 2018 in areas of the campus Loma Linda at the Santo Tomas University. It was evaluated whether the forest interior, forest border and grassland areas constitute habitats that support the establishment of frugivorous birds. The key factors that affect the richness, diversity and abundance of bird species were identified, from which recommendations were made for the establishment of conservation measures. The richness of birds and their abundance was positively correlated with intermediate values of plant richness and diversity and with the presence and coverage of species of palms and lianas. The abundance and diversity of frugivorous birds were higher in border areas and lower in grasslands and forest interiors. Apparently the diversity of the habitat in border areas of fragmented lands increases the diversity of frugivorous birds through an increase in the abundance values. Artificial enrichment is recommended with different tree, shrubs and palms species planted in the edge and interior areas of the forest, and the maintenance of remaining trees and shrubs in the areas of shrub grasslands. Keywords : fragmentation, frugivorous-birds, diversity, natural regeneration.
Structural and magnetic versatility of the manganese(II)/copper complex[Cu(X4obbz)]2- system (X4obbz = oxamido-N,N'-bis(3,5-dihalogenobenzoato); X = Cl, Br). Crystal structures and magnetic properties of MnCu(Cl4obbz)(H2O)5 and MnCu(Br4obbz)(H2O)3.cntdot.2.5H2O

The two compounds MnCu(Cl 4 obbz)(H 2 O) 5 and MnCu(Br 4 obbz)(H 2 O) 3 •2.5H 2 O have been synthesized. Cl 4 obbz stands for oxamido-N,N'-bis(3,5-dichlorobenzoato) and Br 4 obbz for oxamido-N,N'-bis(3,5-dibromobenzoato). Their crystal structures have been determined. The tetrachloro derivative crystallizes in the monoclinic system, space group P2 1 /n
The Warburg Effect: A computational cancer modeling approach

The approach of complex systems has been increasingly used by the different areas of knowledge, such as physics, biology, social sciences, and economy. In studies started in the 1920s that resulted in the publication of a paper in 1956, Warburg was one of the ﬁrst to study the origin of tumorigenic cells, changing the metabolic process of oxidative phosphorylation to glycolysis in cellular respiration, and its implications in the control and proliferation of tumor cells. In this report, we propose a computational method to study the Warburg effect; the competitive advantage of different strains; and cooperation mechanisms in the uptake of nutrients used for energy generation. The objective here is to discuss the trade-off between high-yielding organisms and high reproduction rate. We propose a model to study the competition between normal and tumor cells exposed to hypoxia, or low presence of oxygen, and a more acidic environment. We qualitatively predict the invasion of tumor cells for a broad range of parameter values. This is useful to calibrate a model with real data.
Effect of Lithium Ions on Rheology and Interfacial Forces in Ethylammonium Nitrate and Ethanolammonium Nitrate

The effect of added Li+ to two ionic liquids (ILs), ethylammonium nitrate (EAN) and ethanolammonium nitrate (EtAN), has been investigated using rheology and colloidal probe atomic force microscopy (AFM). Rheology data revealed a complex viscosity dependence that can be ascribed to the different bulk nanostructures. AFM force curves revealed steps for the neat ILs, analogous to those in previous studies. The addition of Li+ broadened the steps, which is likely an effect of ion clusters formed. Friction measurements corroborate this data and also showed that the structure of EtAN is much more prone to change as Li+ is added. These results demonstrate the complex behavior of ILs on interfaces and the effect of perturbing such interactions.
Microbial Spoilage of Fruits: A Review on Causes and Prevention Methods

ABSTRACT Fruits production and consumption are generally increasing because of consumers’ awareness of healthy nutrition. Fruit quality and safety are both important since fruits are vulnerable to microbial deterioration due to their nutritional composition. Spoilage microorganisms invade fruits during the period of growth and mainly during post-harvest handling. Physical damage of the fruit tissue may lead to microbial contamination. Fortunately, fruit spoilage is preventable and this discussion intends to provide an overview of the sources, distinctive factors, and causes of fruit spoilage and some of the commonly adopted methods and practices to prevent fruit spoilage.
Ant Colony Optimization Implementation on Traveling Salesman Problem to Achieve the Shortest Logistic Route

Transportation refers to the movement of everything from raw materials to finished goods between various facilities in the supply chain. In transportation, the exchange between responsiveness and efficiency is manifested in the choice of transportation modes. Because transportation costs can be as much as one third of the supply chain operating costs, the decision made here is very important. Traveling Salesman Problem is one of the best-known NP-hard problems where there is no precise algorithm to solve it in polynomial time. The ACO algorithm has good potential for problem solving and recent research that has attracted a lot of attention, in particular is the case of solving NP-Hard problems. One of the earliest best works is completing TSP using ACS (Ant Colony System). Ant System is the first ACO algorithm with its main characteristics being that at each iteration, the pheromone values are updated by all (m) ants who have built a solution in the iteration itself. From the arrangement of the shipping routes that have been implemented by the Ant Colony Optimization on the Traveling Salesman Problem, the amount of savings in the transportation mode of trucks to mileage is 37%.
RATE OF AWARENESS OF THE HEALTH BENEFIT AND CONSUMPTION OF MORINGA IN NASARAWA, NIGERIA

Moringa is frequently considered as significant starvation food as a result of its high protection from dry season and bone-dry conditions attributable to their tuberous roots. It has acquired its name as the supernatural occurrence tree because of its stunning recuperating capacities for different sicknesses and even some constant illnesses. The study evaluated the Rate of awareness of the health benefit and consumption of moringa in Nasarawa, Nigeria. Cross-sectional data from the primary source was used for this study. Two hundred and ten (210) individuals responded to the survey hence that constitutes the sample size used for the study. Descriptive statistics were used to analyze the data set. From the result the mean age was 34.5 years, most (71.42%) of the respondents were male and married. Most (52.38) of the respondents were civil servants. From the results, the rate of awareness about moringa was calculated to be 0.9047 (90.47). Only 57.14% percent consume moringa leaf or any of the part despite the health benefit. The study concluded that the rate of awareness about moringa was high however the consumption rate was low because of difficulty of access. It was therefore recommended that an advocacy and awareness campaign be carried out to encourage urban dwellers to plant moringa as part of their ornamental crops in their compound, this will ease the difficult encounter in accessing the tree.
Safety Nearby and Financial Welfare: Common Barriers to Safer Neighborhood and Financial Welfare

Crimes are prevalent in human societies. This article examines common barriers in accessing safer neighborhood and financial welfare. The two are likely to share some commonality since socioeconomic factors may underlie. Using individual survey data of the World Values Survey, my results show that socio-economic resources (e.g., income, social class, poverty, and savings) indeed touch not only financial satisfaction, but also neighborhood safety. The results of this paper offer important policy implications. That is, there may be some common efforts which can improve the joint inequalities in accessing safe living environment and sense of financial welfare.
Protective role of Populus tomentiglandulosa against hydrogen peroxide-induced oxidative stress in SH-SY5Y neuronal cells

Oxidative stress caused by the overproduction of reactive oxygen species (ROS) is known as an etiology of neurodegenerative diseases. Populus tomentiglandulosa (PT), a member of the Salicaceae family, is widely grown in Korea and has been reported to exert protective effects on cerebral ischemia by attenuating of oxidative stress and neuronal damage. In the present study, we investigated the antioxidant activity and neuroprotective effects of an ethanol extract and four fractions [ n -butanol, ethyl acetate (EtOAc), chloroform, and n -hexane] of PT under in vitro and cellular systems. The extract and four fractions of PT showed 1,1-diphenyl-2-picrylhydrazyl (DPPH), (cid:129) OH, and O 2 − radical scavenging activities in a dose-dependent manner. In particular, the EtOAc fraction of PT had the strongest DPPH, (cid:129) OH, and O 2 − radical scavenging activities among the extract and other fractions. Therefore, we further investigated the neuroprotective effect of the EtOAc fraction of PT against oxidative stress in H 2 O 2 -induced SH-SY5Y cells. Treatment with H 2 O 2 significantly decreased cell viability and lactate dehydrogenase (LDH) release, and it also increased the ROS levels compared to the normal group. However, treatment with the EtOAc fraction of PT significantly increased cell viability. Moreover, the EtOAc fraction of PT-treated group significantly suppressed ROS production and LDH release compared to the H 2 O 2 -induced control group. In conclusion, our findings indicated that PT had in vitro antioxidant activity and neuroprotective effects against oxidative stress. Therefore, PT could be used as a natural agent for protection against oxidative stress.
Wage Setting Behaviour of Firms in Industrial Estates of Khyber Pakhtunkhwa, Pakistan

The objective of the study is to analyze the wage setting behaviour of firms. For this study four major industrial estates of Khyber Pakhtunkhwa. Using stratified random sampling, data is collected from 342 firms. Multinomial logistic model is used to estimate the effects of determinants on wage change. Most of the firms change wage rate once in a year. Majority of the firms follow time-dependent wage policy. Half of the firms, which are following time-dependent wage policy, change the wage rate at the end or start of the fiscal year. Most of the firms are not found to index wages. Moreover, the percentage of firms not involved in wage indexation is higher for daily workers than for permanent worker. Labor productivity, employment level in the economy, government regulations, taxes, demand for the product, and inflation are important factors of wage change, but the most important factor is the labour productivity and least important is pressure from the labour union. Imperfect competition and size of the firms are the important determinants of wage flexibility, while wage indexation, information set of expected inflation and fringe benefits provided to workers are the important determinants of wage rigidity. It is important for Pakistan to avoid wage cuts at the times of recession and disinflationary policies be carefully designed as sacrifice ratio, albeit moderate, is not zero.