Miracle-dz/UniFAR-FTUs huggingface dataset:
Does Poststroke Lower-Limb Spasticity Influence the Recovery of Standing Balance Control? A 2-Year Multilevel Growth Model

Background. Poststroke lower-limb spasticity (LLS) has been shown to degrade standing balance control by disrupting the temporal synchronization between individual limb centers of pressure (COPs). Time-varying changes in standing balance control associated with alterations in the extent of LLS have yet to be documented and are important to informing treatment strategies to improve such functional outcomes. Objective. The present work aimed to understand the natural recovery of standing balance control among stroke survivors with LLS using limb-specific indices of standing balance control. Furthermore, we sought to understand if time-varying changes in LLS were associated with alterations in standing balance control. Methods. A retrospective analysis of 92 participants was performed; 47 participants never exhibited LLS during the study (No_LLS), and 45 participants exhibited LLS during at least 1 testing session (LLS). Quiet standing for a duration of 30 s on 2 force platforms was recorded. Temporal synchrony and spatial symmetry of COP displacements were assessed, along with interlimb weight-bearing symmetry. Results. All variables, except spatial symmetry, indicated initial improvement followed by deceleration in the rate of balance control recovery. Limb-specific measures indicated that individuals with LLS exhibited deficits in balance control. The recovery trajectories were not different between groups, suggesting a similar rate, but reduced extent, of balance control recovery among the LLS relative to the No_LLS group. Only temporal synchrony was altered by time-varying changes in spasticity. Conclusions. The present results suggest that the reduction in spasticity may be beneficial to balance control recovery.
Effects of Unilateral Upper Limb Training in Two Distinct Prognostic Groups Early After Stroke

Background and Objective. Favorable prognosis of the upper limb depends on preservation or return of voluntary finger extension (FE) early after stroke. The present study aimed to determine the effects of modified constraint-induced movement therapy (mCIMT) and electromyography-triggered neuromuscular stimulation (EMG-NMS) on upper limb capacity early poststroke. Methods. A total of 159 ischemic stroke patients were included: 58 patients with a favorable prognosis (>10° of FE) were randomly allocated to 3 weeks of mCIMT or usual care only; 101 patients with an unfavorable prognosis were allocated to 3-week EMG-NMS or usual care only. Both interventions started within 14 days poststroke, lasted up until 5 weeks, focused at preservation or return of FE. Results. Upper limb capacity was measured with the Action Research Arm Test (ARAT), assessed weekly within the first 5 weeks poststroke and at postassessments at 8, 12, and 26 weeks. Clinically relevant differences in ARAT in favor of mCIMT were found after 5, 8, and 12 weeks poststroke (respectively, 6, 7, and 7 points; P < .05), but not after 26 weeks. We did not find statistically significant differences between mCIMT and usual care on impairment measures, such as the Fugl-Meyer assessment of the arm (FMA-UE). EMG-NMS did not result in significant differences. Conclusions. Three weeks of early mCIMT is superior to usual care in terms of regaining upper limb capacity in patients with a favorable prognosis; 3 weeks of EMG-NMS in patients with an unfavorable prognosis is not beneficial. Despite meaningful improvements in upper limb capacity, no evidence was found that the time-dependent neurological improvements early poststroke are significantly influenced by either mCIMT or EMG-NMS.
Exploitation of periodicity in logic simulation of synchronous circuits

An overwhelming majority of logic designers use synchronous logic design techniques to manage the complexity of their designs and rely on logic simulation techniques for design verification. Yet, logic simulators do not take advantage of the higher abstraction level provided by synchronous logic design techniques to improve their performance. A general technique is presented which takes advantage of the high degree of periodicity common in synchronous logic designs. It is shown that a performance improvement of at least 200% occurs when these techniques are applied within the COSMOS. simulation system to simulate large digital systems. <<ETX>>
Secretion of Flagellar Proteins by the Pseudomonas aeruginosa Type III Secretion-Injectisome System

ABSTRACT The opportunistic pathogen Pseudomonas aeruginosa utilizes an injectisome-type III secretion system (injectisome-T3SS) to elicit cytotoxicity toward epithelial cells and macrophages. Macrophage killing results from the cytotoxic properties of the translocated effector proteins (ExoS, ExoT, ExoU, and ExoY) and inflammasome-mediated induction of pyroptosis. Inflammasome activation can occur following Nlrc4-mediated recognition of cytosolic translocated flagellin (FliC). In the present study, we demonstrate that FliC is a secretion substrate of both the injectisome- and flagellum-associated T3SSs. Molecular analyses indicate that the first 20 amino-terminal residues of FliC are sufficient for secretion by the injectisome-T3SS and that the first 100 residues are sufficient for translocation of FliC into host cells. Although maximal inflammasome activation requires FliC, activation can also occur in the absence of FliC. This prompted us to examine whether other flagellar components might also be translocated into cells to elicit inflammasome activation. Indeed, we find that the flagellar cap (FliD), hook-associated (FlgK and FlgL), hook (FlgE), and rod (FlgE) proteins are secretion substrates of the injectisome-T3SS. None of these proteins, however, result in increased inflammasome activation when they are overexpressed in a fliC mutant and appear to be translocated into host cells. While a role in inflammasome activation has been excluded, these data raise the possibility that flagellar components, which are highly conserved between different bacterial species, trigger other specific host responses from the extracellular milieu or contribute to the pathogenesis of P. aeruginosa. IMPORTANCE The inflammasome is a host defense mechanism that recognizes invading bacteria and triggers an inflammatory immune response. The opportunistic pathogen P. aeruginosa produces both inflammasome agonists and antagonists. In this study, we demonstrate that overexpression of an agonist suppresses the activity of an antagonist, thereby resulting in inflammasome activation. Since the relative expression levels of agonists and antagonists likely vary between strains, these differences could be important predictors of whether a particular P. aeruginosa strain elicits inflammasome activation.
MIS-MV: optimization of multi-level logic with multiple-values inputs

Techniques are presented for the optimization of multi-level logic with multiple-valued input variables. The motivation for this is to tackle the input encoding problem in logic synthesis, where binary codes need to be found for the different values of a symbolic input variable. Multi-level multiple-valued optimization is used to generate constraints that are used to determine the codes. The state assignment problem in sequential logic synthesis can be approximated as an input encoding problem by ignoring the next state field, which is reasonable when the primary output logic, dominates the next state logic. A novel technique is presented for extracting common factors with multiple-valued variables, and it is shown how other multi-level optimization techniques are easily extended with multiple-valued variables. These ideas have been implemented as algorithms in the MIS-MV program. Practical issues are also presented regarding implementation. Experimental results are also given. <<ETX>>
More than One Way To Control Hair Growth: Regulatory Mechanisms in Enterobacteria That Affect Fimbriae Assembled by the Chaperone/Usher Pathway

ABSTRACT Many Gram-negative enterobacteria produce surface-associated fimbriae that facilitate attachment and adherence to eucaryotic cells and tissues. These organelles are believed to play an important role during infection by enabling bacteria to colonize specific niches within their hosts. One class of these fimbriae is assembled using a periplasmic chaperone and membrane-associated scaffolding protein that has been referred to as an usher because of its function in fimbrial biogenesis. The presence of multiple types of fimbriae assembled by the chaperone/usher pathway can be found both within a single bacterial species and also among different genera. One way of controlling fimbrial assembly in these bacteria is at the genetic level by positively or negatively regulating fimbrial gene expression. This minireview considers the mechanisms that have been described to control fimbrial gene expression and uses specific examples to demonstrate both unique and shared properties of such regulatory mechanisms.
Clinician’s Road Map to Wavelet EEG as an Alzheimer’s disease Biomarker

Alzheimer’s disease (AD) is considered the main cause of dementia in Western countries. Consequently, there is a need for an accurate, universal, specific and cost-effective biomarker for early AD diagnosis, to follow disease progression and therapy response. This article describes a new diagnostic approach to quantitative electroencephalogram (QEEG) diagnosis of mild and moderate AD. The data set used in this study was composed of EEG signals recorded from 2 groups: (S1) 74 normal subjects, 33 females and 41 males (mean age 67 years, standard deviation = 8) and (S2) 88 probable AD patients (NINCDS-ADRDA criteria), 55 females and 33 males (mean age 74.7 years, standard deviation = 7.8) with mild to moderate symptoms (DSM-IV-TR). Attention is given to sample size and the use of state of the art open source tools (LetsWave and WEKA) to process the EEG data. This innovative technique consists in associating Morlet wavelet filter with a support vector machine technique. A total of 111 EEG features (attributes) were obtained for 162 probands. The results were accuracy of 92.72% and area under the curve of 0.92 (percentage split test). Most important, comparing a single patient versus the total data set resulted in accuracy of 84.56% (leave-one-patient-out test). Particular emphasis was on clinical diagnosis and feasibility of implementation of this low-cost procedure, because programming knowledge is not required. Consequently, this new method can be useful to support AD diagnosis in resource-limited settings.
Long-Term Evolution Electromagnetic Fields Exposure Modulates the Resting State EEG on Alpha and Beta Bands

Long-term evolution (LTE) wireless telecommunication systems are widely used globally, which has raised a concern that exposure to electromagnetic fields (EMF) emitted from LTE devices can change human neural function. To date, few studies have been conducted on the effect of exposure to LTE EMF. Here, we evaluated the changes in electroencephalogram (EEG) due to LTE EMF exposure. An LTE EMF exposure system with a stable power emission, which was equivalent to the maximum emission from an LTE mobile phone, was used to radiate the subjects. Numerical simulations were conducted to ensure that the specific absorption rate in the subject’s head was below the safety limits. Exposure to LTE EMF reduced the spectral power and the interhemispheric coherence in the alpha and beta bands of the frontal and temporal brain regions. No significant change was observed in the spectral power and the inter-hemispheric coherence in different timeslots during and after the exposure. These findings also corroborated those of our previous study using functional magnetic resonant imaging.
Correlation Between Bispectral Index and Electrocorticographic Features During Epilepsy Surgery

Surgical resection guided by intraoperative electrocorticography (iECoG) has been in clinical use for many decades. The use of the bispectral index (BIS) for monitoring depth of anesthesia during different types of surgery, including epilepsy surgery, is increasing nowadays. The BIS is an EEG-derived variable indicating cortical electrical activity. However, the correlation between the BIS score and the iECoG score, with the purpose of optimizing the quality and time of the iECoG recordings in epilepsy surgery is unknown. The goal of this study was to evaluate the correlation between BIS values and iECoG parameters during the epilepsy surgery under anesthesia with propofol and fentanyl. This is a prospective study that included patients with epilepsy who underwent epilepsy surgery guided by BIS and iECoG (September 2008 to October 2013). Clinical, physiological, and sociodemographic characteristics are shown. We correlated the iECoG parameters (presence of burst suppressions [BS], suppression time [seconds], background frequency [Hz], and type of iECoG score by Mathern et al) with BIS values. We included 28 patients, 15/28 (53.5%) female, general mean age of 30.5 years (range 13-56 years). Patients underwent epilepsy surgery: 22/28 (79%) temporal and 6/28 (21%) extratemporal. We found a nonlinear polynomial cubic relationship between the mentioned variables noting that a BIS range of 40 to 60 gave the following results: iECoG BS periods <5 seconds, background frequency 10 to 17 Hz, and iECoG score 2 characterized by lack of >20-Hz background frequencies. No BS were observed with a BIS > 60. In conclusion BIS values and iECoG parameters during the epilepsy surgery under anesthesia with propofol and fentanyl have a nonlinear correlation. BS patterns were not found with a BIS > 60. These findings show that BIS is a nonlinear multidimensional measure, which possesses high variability with the iECoG parameters. BS patterns are not found with BIS > 60.
Stigmatization of Overweight and Obese Individuals: Implications for Mental Health Promotion

In the United States obesity has been considered an epidemic, with approximately 72.5 million adults classified as obese. Despite the increasing prevalence of obesity, stigmatization of obese individuals has not decreased. There is a widespread stereotype of and negative attitude toward overweight and obese individuals. Stigmatization of these individuals has led to discrimination, as well as feelings of shame and guilt. Although much has been written about obesity, the goals for prevention, and strategies for behavior change, there are few guidelines for practitioners who are working with overweight and obese individuals to minimize the effects stigma might have on overall mental health and consequent behavior change strategies. The purpose of this review is to help guide those working with overweight and obese individuals to understand more fully stigmatization and the role it may play in their approach to treatment.
Structure of Compositionally Simple Lipopolysaccharide from Marine Synechococcus

ABSTRACT Lipopolysaccharide (LPS) is the first defense against changing environmental factors for many bacteria. Here, we report the first structure of the LPS from cyanobacteria based on two strains of marine Synechococcus, WH8102 and CC9311. While enteric LPS contains some of the most complex carbohydrate residues in nature, the full-length versions of these cyanobacterial LPSs have neither heptose nor 3-deoxy-d-manno-octulosonic acid (Kdo) but instead 4-linked glucose as their main saccharide component, with low levels of glucosamine and galacturonic acid also present. Matrix-assisted laser desorption ionization mass spectrometry of the intact minimal core LPS reveals triacylated and tetraacylated structures having a heterogeneous mix of both hydroxylated and nonhydroxylated fatty acids connected to the diglucosamine backbone and a predominantly glucose outer core-like region for both strains. WH8102 incorporated rhamnose in this region as well, contributing to differences in sugar composition and possibly nutritional differences between the strains. In contrast to enteric lipid A, which can be liberated from LPS by mild acid hydrolysis, lipid A from these organisms could be produced by only two novel procedures: triethylamine-assisted periodate oxidation and acetolysis. The lipid A contains odd-chain hydroxylated fatty acids, lacks phosphate, and contains a single galacturonic acid. The LPS lacks any limulus amoebocyte lysate gelation activity. The highly simplified nature of LPSs from these organisms leads us to believe that they may represent either a primordial structure or an adaptation to the relatively higher salt and potentially growth-limiting phosphate levels in marine environments.
Priority-based real-time stream coding over multi-channel under sliding window erasures

Multi-channel technology can be used to provide higher transmission ability to the bandwidth-intensive and delay-sensitive real-time streams. However, traditional channel capacity theories and coding schemes are seldom designed for the real-time streams with strict delay constraint, especially when multiple channels are used. This paper considers a real-time stream system, where real-time messages with different importance should be transmitted through several packet erasure channels, and be decoded by the receiver within a fixed delay. We introduce the concepts of Symmetric Real-time (SR) stream and Asymmetric Real-time (AR) stream according to the stream characteristics. The multi-channel transmission capacities are derived for SR and AR streams under sliding window erasures, which can be used to guide the design of efficient real-time stream coding approaches. A Maximum Equilibrium Intra-session Code (MEIC) is presented for SR and AR streams, and is shown asymptotically able to achieve the corresponding transmission capacities. Finally, the performance of MEIC is verified by simulations.
Inflammation and autoimmunity in patients anti-neutrophil nuclear antibodies positive

Two patients, female, with overlap syndrome AIH/PSC and pANNA positive, was placed at two extremities of the bimodal curve that characterised the AIH I° behaviour.Therefore they show difference of age (V.L. 76 years and E.G. 8 years) and different prognosis and therapy answer: the disease of the young patient has an aggressive outcome and fails the treatment more commonly.The more important theory to explain this different features was the “molecular footprint hypothesis” that considers decisive the type of HLA II°. The two patients considered have the same HLADR3. The serological analysis and the recent pathogenically orientations show that the local motives regulate the features of disease: in the child the inflammatory component prevails and maintains a chronic granulomatous aggression, whereas in the old patient the non specific autoimmune IgG prevails and drives a fibrosis evolution and immunosuppressive therapy response.The common positive pANNA can be expression of common chronic auto-inflammatory granulomatous origin.
Enhancement of chronic cyclosporine nephrotoxicity by sodium depletion in an experimental mouse model

Summary: The major adverse reaction to cyclosporine A (CsA) is chronic nephrotoxicity. to elucidate the pathogenesis of CsA nephrotoxicity and the potential role of the renin‐angiotensin system (RAS) in mediating this toxicity, a reliable mouse model would enable us to take advantage of various probes, and gene knockout animals available in this species, but not in the rat, would be extremely useful. Using the manoeuvre of sodium depletion and activation of RAS, an experimental model in the mouse has been developed to reproduce structural and functional characteristics of chronic CsA nephrotoxicity in humans. Mice were treated with CsA subcutaneously at dosages of 10 mg/kg per day and 30 mg/kg per day or olive oil as a placebo on a low sodium diet (LS) (0.01% sodium) for 28 days. Comparisons were made with mice treated with CsA at 100 mg/kg per day on a normal sodium diet (NS; 0.4% sodium) for 56 days. Cyclosporine A treatment induced significant increases in serum creatinine and blood urea nitrogen (BUN) and a decrease in creatinine clearance in mice on LS (P<0.05 vs placebo). Sodium‐depleted animals on CsA 30mg/kg per day treatment developed significant histological lesions in the kidneys consisting of tubulointerstitial fibrosis, arteriolopathy, nephrocalcinosis, and tubular injury after 28 days (P<0.001 vs placebo). By contrast, CsA treatment in mice on NS did not induce functional and structural abnormalities in the kidneys even up to 56 days at a higher dose. This mouse model can be useful to gain further insights into the pathogenetic mechanisms of chronic CsA nephropathy.
Gene Transfer Efficiency in Gonococcal Biofilms: Role of Biofilm Age, Architecture, and Pilin Antigenic Variation

ABSTRACT Extracellular DNA is an important structural component of many bacterial biofilms. It is unknown, however, to which extent external DNA is used to transfer genes by means of transformation. Here, we quantified the acquisition of multidrug resistance and visualized its spread under selective and nonselective conditions in biofilms formed by Neisseria gonorrhoeae. The density and architecture of the biofilms were controlled by microstructuring the substratum for bacterial adhesion. Horizontal transfer of antibiotic resistance genes between cocultured strains, each carrying a single resistance, occurred efficiently in early biofilms. The efficiency of gene transfer was higher in early biofilms than between planktonic cells. It was strongly reduced after 24 h and independent of biofilm density. Pilin antigenic variation caused a high fraction of nonpiliated bacteria but was not responsible for the reduced gene transfer at later stages. When selective pressure was applied to dense biofilms using antibiotics at their MIC, the double-resistant bacteria did not show a significant growth advantage. In loosely connected biofilms, the spreading of double-resistant clones was prominent. We conclude that multidrug resistance readily develops in early gonococcal biofilms through horizontal gene transfer. However, selection and spreading of the multiresistant clones are heavily suppressed in dense biofilms. IMPORTANCE Biofilms are considered ideal reaction chambers for horizontal gene transfer and development of multidrug resistances. The rate at which genes are exchanged within biofilms is unknown. Here, we quantified the acquisition of double-drug resistance by gene transfer between gonococci with single resistances. At early biofilm stages, the transfer efficiency was higher than for planktonic cells but then decreased with biofilm age. The surface topography affected the architecture of the biofilm. While the efficiency of gene transfer was independent of the architecture, spreading of double-resistant bacteria under selective conditions was strongly enhanced in loose biofilms. We propose that while biofilms help generating multiresistant strains, selection takes place mostly after dispersal from the biofilm.