Miracle-dz/UniFAR-FTUs huggingface dataset:
Stem Cell-Induced Pulp Regeneration Can Be Enhanced by Administration of CCL11-Neutralizing Antibody in the Ectopic Tooth Transplantation Model in the Aged Mice.

OBJECTIVE Pulp regeneration by stem cell transplantation declines due to age-related reduction. We hypothesized that administration of a cytokine together with the cell transplantation may improve the stem cell niche microenvironment and promote regeneration. CCL11 is implicated as a factor in aging. This investigation was performed to investigate the changes in the quality of the regenerated pulp by administration of CCL11 antibody in the aged mice and elucidate the underlying mechanisms. MATERIALS AND METHODS Mobilized dental pulp stem cell (MDPSC) transplants were characterized in an ectopic tooth root transplantation model in both the aged and young mice. The amount of regenerated pulp tissue was analyzed in the transplants with continuous administration of CCL11 antibody compared with those without the antibody administration. Blood CCL11 levels were assessed at the onset of the experiment. Furthermore, immunostaining of CD68 together with CD11c or CD206 for M1 and M2 macrophage, respectively, were performed. Each double-positive cell count of M1 and M2 macrophages and M1/M2 ratio in the transplants with administration were compared with those without administration both in the aged and young mice. RESULTS The administration of CCL11 antibody enhanced pulp regeneration and significantly reduced the blood CCL11 level in the aged mice. As the number of M1 macrophages decreased, the M1/M2 ratio in the treated aged mouse was less than that in the untreated aged mouse. There was, however, significant difference between the treated aged mouse and the untreated young mouse. CONCLUSION CCL11 antibody has the potential to enhance and stimulate pulp regeneration in the aged mice.
p19-Targeting ILP Protein Blockers of IL-23/Th-17 Pro-Inflammatory Axis Displayed on Engineered Bacteria of Food Origin

IL-23-mediated Th-17 cell activation and stimulation of IL-17-driven pro-inflammatory axis has been associated with autoimmunity disorders such as Inflammatory Bowel Disease (IBD) or Crohn’s Disease (CD). Recently we developed a unique class of IL-23-specific protein blockers, called ILP binding proteins that inhibit binding of IL-23 to its cognate cell-surface receptor (IL-23R) and exhibit immunosuppressive effect on human primary blood leukocytes ex vivo. In this study, we aimed to generate a recombinant Lactococcus lactis strain which could serve as in vivo producer/secretor of IL-23 protein blockers into the gut. To achieve this goal, we introduced ILP030, ILP317 and ILP323 cDNA sequences into expression plasmid vector containing USP45 secretion signal, FLAG sequence consensus and LysM-containing cA surface anchor (AcmA) ensuring cell-surface peptidoglycan anchoring. We demonstrate that all ILP variants are expressed in L. lactis cells, efficiently transported and secreted from the cell and displayed on the bacterial surface. The binding function of AcmA-immobilized ILP proteins is documented by interaction with a recombinant p19 protein, alpha subunit of human IL-23, which was assembled in the form of a fusion with Thioredoxin A. ILP317 variant exhibits the best binding to the human IL-23 cytokine, as demonstrated for particular L.lactis-ILP recombinant variants by Enzyme-Linked ImmunoSorbent Assay (ELISA). We conclude that novel recombinant ILP-secreting L. lactis strains were developed that might be useful for further in vivo studies of IL-23-mediated inflammation on animal model of experimentally-induced colitis.
Presenting Symptoms Independently Predict Mortality in Septic Shock: Importance of a Previously Unmeasured Confounder*

Objectives: Presenting symptoms in patients with sepsis may influence rapidity of diagnosis, time-to-antibiotics, and outcome. We tested the hypothesis that vague presenting symptoms are associated with delayed antibiotics and increased mortality. We further characterized individual presenting symptoms and their association with mortality. Design: Retrospective cohort study. Setting: Emergency department of large, urban, academic U.S. hospital. Patients: All adult patients with septic shock treated in the emergency department between April 2014 and March 2016. Interventions: None. Measurements and Main Results: Of 654 septic shock cases, 245 (37%) presented with vague symptoms. Time-to-antibiotics from first hypotension or elevated lactate was significantly longer for those with vague symptoms versus those with explicit symptoms of infection (1.6 vs 0.8 hr; p < 0.01), and in-hospital mortality was also substantially higher (34% vs 16%; p < 0.01). Patients with vague symptoms were older and sicker as evidenced by triage hypotension, Sequential Organ Failure Assessment score, initial serum lactate, and need for intubation. In multivariate analysis, vague symptoms were independently associated with mortality (adjusted odds ratio, 2.12; 95% CI, 1.32–3.40; p < 0.01), whereas time-to-antibiotics was not associated with mortality (adjusted odds ratio, 1.01; 95% CI, 0.94–1.08; p = 0.78). Of individual symptoms, only the absence of fever, chills, or rigors (odds ratio, 2.70; 95% CI, 1.63–4.47; p < 0.01) and presence of shortness of breath (odds ratio, 1.97; 95% CI, 1.23–3.15; p < 0.01) were independently associated with mortality. Conclusions: More than one third of patients with septic shock presented to the emergency department with vague symptoms that were not specific to infection. These patients had delayed antibiotic administration and higher risk of mortality even after controlling for demographics, illness acuity, and time-to-antibiotics in multivariate analysis. These findings suggest that the nature of presenting symptoms is an important component of sepsis clinical phenotyping and may be an important confounder in sepsis epidemiologic studies.
Viral glycoproteomes: technologies for characterization and outlook for vaccine design

It has long been known that surface proteins of most enveloped viruses are covered with glycans. It has furthermore been demonstrated that glycosylation is essential for propagation and immune evasion for many viruses. The recent development of high‐resolution mass spectrometry techniques has enabled identification not only of the precise structures but also the positions of such post‐translational modifications on viruses, revealing substantial differences in extent of glycosylation and glycan maturation for different classes of viruses. In‐depth characterization of glycosylation and other post‐translational modifications of viral envelope glycoproteins is essential for rational design of vaccines and antivirals. In this Review, we provide an overview of techniques used to address viral glycosylation and summarize information on glycosylation of enveloped viruses representing ongoing public health challenges. Furthermore, we discuss how knowledge on glycosylation can be translated to means to prevent and combat viral infections.
Clinical significance of PDEF factor expression and its relation to androgen receptor in ER− breast cancer

The mechanism of androgen receptor (AR) promoting tumour growth in oestrogen receptor‐negative (ER−) breast cancer (BC) is undetermined. Prostate‐derived ETS factor (PDEF) is highly restricted to the hormone‐regulated tissues of epithelial cells, such as those in the prostate, breast and other tissues. It has been demonstrated that PDEF expression is associated with AR in prostate cancer. In this research, we aimed to investigate the relationship between PDEF and AR in ER− BC.
High efficiency triple-stacked class-E power amplifier with novel dynamic biasing network

In this paper, we present a 5-GHz high efficiency class-E power amplifier. Due to zero voltage switching and zero voltage derivative switching conditions, class-E power amplifiers (PAs) are highly efficient. But the reliability of device caused by excessively high peak voltages should be considered carefully. In order to alleviate the device stress, a triple-stacked class-E PA with proposed dynamic biasing network is presented, which can divide the voltage stress equally among three transistors. The supply voltage of the proposed PA is increased compared to common source or cascode class-E PA, which enhances the efficiency of class-E PA. Because of the high supply voltage and operating frequency of 5 GHz, the proposed class-E PA requires small shunt capacitance, which limits the transistor size. The proposed parallel resonant inductor breaks this limitation and avoids the power loss caused by the large on-resistance of small-sized transistors. The proposed PA is implemented in a 0.18 μm CMOS process with the chip area of 0.235 mm2. Simulation results show that the PA achieves 49.5% power-added efficiency (PAE) and 53.7% drain efficiency while delivering peak output power of 22.3 dBm at 5 GHz.
Chromatin organization by an interplay of loop extrusion and compartmental segregation

Significance Human DNA is 2 m long and is folded into a 10-μm-sized cellular nucleus. Experiments have revealed two major features of genome organization: Segregation of alternating active and inactive regions into compartments, and formation of compacted local domains. These were hypothesized to be formed by different mechanisms: Compartments can be formed by microphase separation and domains by active, motor-driven, loop extrusion. Here, we integrate these mechanisms into a polymer model and show that their interplay coherently explains diverse experimental data for wild-type and mutant cells. Our results provide a framework for the interpretation of chromosome organization in cellular phenotypes and highlight that chromatin is a complex, active matter shaped by an interplay of phase segregation and loop extrusion. Mammalian chromatin is spatially organized at many scales showing two prominent features in interphase: (i) alternating regions (1–10 Mb) of active and inactive chromatin that spatially segregate into different compartments, and (ii) domains (<1 Mb), that is, regions that preferentially interact internally [topologically associating domains (TADs)] and are central to gene regulation. There is growing evidence that TADs are formed by active extrusion of chromatin loops by cohesin, whereas compartmentalization is established according to local chromatin states. Here, we use polymer simulations to examine how loop extrusion and compartmental segregation work collectively and potentially interfere in shaping global chromosome organization. A model with differential attraction between euchromatin and heterochromatin leads to phase separation and reproduces compartmentalization as observed in Hi-C. Loop extrusion, essential for TAD formation, in turn, interferes with compartmentalization. Our integrated model faithfully reproduces Hi-C data from puzzling experimental observations where altering loop extrusion also led to changes in compartmentalization. Specifically, depletion of chromatin-associated cohesin reduced TADs and revealed finer compartments, while increased processivity of cohesin strengthened large TADs and reduced compartmentalization; and depletion of the TAD boundary protein CTCF weakened TADs while leaving compartments unaffected. We reveal that these experimental perturbations are special cases of a general polymer phenomenon of active mixing by loop extrusion. Our results suggest that chromatin organization on the megabase scale emerges from competition of nonequilibrium active loop extrusion and epigenetically defined compartment structure.
Clinical evaluation and thermal and chemical characterization of hydrocolloid dressing

This study aimed to evaluate clinically and technologically the hydrocolloid, which is a polymeric material used to prevent nasal injury in newborns (NBs) submitted to noninvasive ventilation (NIV). The study was divided into two stages. In the first, a control case study was performed with 11 NBs hospitalized at the Neonatal Intensive Care Units (NICUs) of a public hospital in the South of Brazil, and who required NIV with nasal hydrocolloid protection. The stage of the injury and the need for exchanging nasal protection were recorded daily until the suspension of NIV. In the second stage, a hydrocolloid sample was subjected to Fourier Transform Infrared Spectroscopy (FTIR) and Thermogravimetric Analysis (TGA). The incidence of the injury was 36.36%, all of them being stage I. The hydrocolloid showed no detachment during the use, and did not need to be replaced. As for FTIR, the adhesive surface presented bands with characteristics of a thermoplastic elastomer and sodium carboxymethylcellulose. And the non-adhesive surface showed characteristics of a polyether urethane thermoplastic elastomer. In the TGA, the hydrocolloid presented thermal stability up to 100° C. So, we concluded that the hydrocolloid is an effective protection to prevent the nasal injury presenting good adhesion to the nasal base of the NB.
Communications for sanitary teleservices in a smart city

A Smart City is characterized by being a desirable place to live and that encourages initiatives that affect the well-being of citizens, anticipating their needs in an efficient, flexible and sustainable way. This work describes a scenario of improvement in the quality of care of the citizens in a Smart City environment through the intensive use of communications, devices and specific applications. For the evaluation of benefits of this hypothetical scenario, a tool developed on the OMNET ++ simulation environment called “SimulCity” will be used, that will allow evaluating the quality of service offered to users. Finally, results of this scenario will be shown.
The Changes of CTX, DPD, Osteocalcin, and Bone Mineral Density During the Postmenopausal Period

Objective To investigate appropriate treatment time and useful bone turnover markers (BTMs) for monitoring bone turnover during the postmenopausal period, we analyzed changes of two bone resorption markers; serum carboxyterminal telopeptide of collagen I (s-CTX), urine deoxypyridinoline (u-DPD), one bone formation marker; serum osteocalcin (s-OC), and bone mineral density (BMD) in Korean postmenopausal women. Methods Seventy-eight menopausal women were divided into three groups according to postmenopausal period: group I (0–5 years), group II (6–10 years), group III (≥10 years). All groups were subdivided into an osteoporosis group (T-score≤-2.5) and a non-osteoporosis group (T-score>-2.5). BTMs such as s-CTX, u-DPD, s-OC, and BMD (g/cm2) were measured by dual-energy X-ray absorptiometry (DXA) in all patients. Analysis of variables among groups based on the postmenopausal period was performed using ANOVA. Results There was significant negative correlation between BMD and postmenopausal period. The levels of all BTMs including s-CTX, u-DPD, and s-OC were highest in group II and the increased levels of all BTMs subsequently declined in group III. The levels of BTMs were higher in the osteoporosis groups than in the non-osteoporosis groups in all subjects. It was statistically significant that the level of s-CTX in group I was higher in the osteoporosis group than in the non-osteoporosis group. Conclusion This study showed that bone resorption and bone formation were the highest 5–10 years after menopause, and s-CTX is more useful than u-DPD among the bone resorption markers. It’s important to measure serially both BMD and BTM within 10 years after menopause for accurate diagnosis and management for postmenopausal osteoporosis.
How Healthy Lifestyle Factors at Midlife Relate to Healthy Aging

With increasing life expectancies worldwide, it is an important public health issue to identify factors that influence the quality of aging. We aimed to investigate the individual and combined roles of lifestyle factors at midlife for healthy aging (HA). We analyzed data from 2203 participants of the French ”Supplémentation en Vitamines et Minéraux Antioxydants” (SU.VI.MAX) cohort aged 45–60 years at baseline (1994–1995), and assessed the combined impact of lifestyle factors (weight, smoking status, physical activity, alcohol consumption, and diet) on HA (absence of chronic diseases and function-limiting pain, good physical and cognitive functioning, functional independence, no depressive symptoms, and good social and self-perceived health) with a five-component healthy lifestyle index (HLI). Relative risks (RR) and 95% confidence intervals (CIs) were estimated using a robust-error-variance Poisson regression. Approximately 39% of our sample aged healthily. After adjustment for potential confounders, a one-point increase in HLI was related to an 11% higher probability of HA (95% CI = 6%, 16%; p < 0.001). The proportions of HA attributable to specific factors based on the “population attributable risk” concept were 7.6%, 6.0%, 7.8%, and 16.5% for body mass index (BMI), physical activity, diet quality, and smoking status, respectively. This study highlights the importance of healthy lifestyle habits at midlife for the promotion of good overall health during aging.
Hemochromatosis: pathophysiology, evaluation, and management of hepatic iron overload with a focus on MRI

ABSTRACT Introduction: Hereditary hemochromatosis (HH) is an autosomal recessive disorder that occurs in approximately 1 in 200–250 individuals. Mutations in the HFE gene lead to excess iron absorption. Excess iron in the form of non-transferrin-bound iron (NTBI) causes injury and is readily uptaken by cardiomyocytes, pancreatic islet cells, and hepatocytes. Symptoms greatly vary among patients and include fatigue, abdominal pain, arthralgias, impotence, decreased libido, diabetes, and heart failure. Untreated hemochromatosis can lead to chronic liver disease, fibrosis, cirrhosis, and hepatocellular carcinoma (HCC). Many invasive and noninvasive diagnostic tests are available to aid in diagnosis and treatment. MRI has emerged as the reference standard imaging modality for the detection and quantification of hepatic iron deposition, as ultrasound (US) is unable to detect iron overload and computed tomography (CT) findings are nonspecific and influenced by multiple confounding variables. If caught and treated early, HH disease progression can significantly be altered. Area covered: The data on Hemochromatosis, iron overload, and MRI were gathered by searching PubMed. Expert commentary: MRI is a great tool for diagnosis and management of iron overload. It is safe, effective, and a standard protocol should be included in diagnostic algorithms of future treatment guidelines.
Older adults use a prefrontal regulatory mechanism to reduce negative memory vividness of a highly emotional real-world event

Previous research has revealed an age-related shift in how individuals recall events from their personal past, with older adults reporting events that are more positive than young adults. We recently showed that age-by-valence interactions may be partially driven by a prefrontally mediated control mechanism recruited by older adults during retrieval of negative laboratory events to reduce phenomenological richness. Specifically, age was associated with greater increases in prefrontal recruitment during retrieval of negative relative to positive events, with this recruitment linked to decreases in hippocampal activity and subjective vividness ratings. In the current study, we examined whether older adults may rely on a similar mechanism during retrieval of a complex, highly emotional real-world event. Participants (n=58, age: 18–87 years) were presented with images related to the 2013 Boston Marathon Bombings and were asked to retrieve a memory associated with each image. Images cued participants with either negative (associated with fear, destruction, and sadness) or positive (images of hope, resilience, support) features. This study replicated previous episodic memory tasks: age was associated with more negative hippocampal connectivity with dorsomedial prefrontal regions during retrieval of memories triggered by negative relative to positive cues. Such findings suggest that older adults may be recruiting a similar regulatory mechanism during retrieval of both negative laboratory stimuli and highly negative events from their past. These findings are discussed in relation to previous work showing that young and older adults interact differently with the negative details related to a highly negative event.
A canadian french emotional speech dataset

Until recently, there was no emotional speech dataset available in Canadian French. This was a limiting factor for research activities not only in Canada, but also elsewhere. This paper introduces the newly released Canadian French Emotional (CaFE) speech dataset and gives details about its design and content. This dataset contains six different sentences, pronounced by six male and six female actors, in six basic emotions plus one neutral emotion. The six basic emotions are acted in two different intensities. The audio is digitally recorded at high-resolution (192 kHz sampling rate, 24 bits per sample). This new dataset is freely available under a Creative Commons license (CC BY-NC-SA 4.0).
LiReD: A Light-Weight Real-Time Fault Detection System for Edge Computing Using LSTM Recurrent Neural Networks

Monitoring the status of the facilities and detecting any faults are considered an important technology in a smart factory. Although the faults of machine can be analyzed in real time using collected data, it requires a large amount of computing resources to handle the massive data. A cloud server can be used to analyze the collected data, but it is more efficient to adopt the edge computing concept that employs edge devices located close to the facilities. Edge devices can improve data processing and analysis speed and reduce network costs. In this paper, an edge device capable of collecting, processing, storing and analyzing data is constructed by using a single-board computer and a sensor. And, a fault detection model for machine is developed based on the long short-term memory (LSTM) recurrent neural networks. The proposed system called LiReD was implemented for an industrial robot manipulator and the LSTM-based fault detection model showed the best performance among six fault detection models.
Category-aware hierarchical caching for video-on-demand content on youtube

Content delivery networks (CDNs) carry more than half of the video content in today's Internet. By placing content in caches close to the users, CDNs help increasing the Quality of Experience, e.g., by decreasing the delay until a video playback starts. Existing works on CDN cache performance focus mostly on distinct caching metrics, such as hit rate, given an abstract workload model. Moreover, the nature of the geographical distribution and connection of caches is often oversimplified. In this work, we investigate the performance of cache hierarchies while taking into account the presence of a mixed content workload comprising multiple categories, e.g., news, comedy, and music. We consider the performance of existing caching strategies in terms of cache hit rate and deterioration costs in terms of write operations. Further, we contribute a design and an evaluation of a content category-aware caching strategy, which has the benefit of being sensitive to changing category-specific content popularity. We evaluate our caching strategy, denoted as ACDC (Adaptive Content-Aware Designed Cache), using multiple caching hierarchy models, different cache sizes, and a real world trace covering one week of YouTube requests observed in a large European mobile ISP network. We demonstrate that ACDC increases the cache hit rate for certain hierarchies up to 18.39% and decreases transmission latency up to 12%. Additionally, a decrease in disk write operations up to 55% is observed.