Miracle-dz/UniFAR-FTUs huggingface dataset:
Discharge characteristics of medial rectus and abducens motoneurons in the goldfish.

1. The discharge of antidromically identified medial rectus and abducens motoneurons was recorded in restrained unanesthesized goldfish during spontaneous eye movements and in response to vestibular and optokinetic stimulation. 2. All medial rectus and abducens motoneurons exhibited a similar discharge pattern. A burst of spikes accompanied spontaneous saccades and fast phases during vestibular and optokinetic nystagmus in the ON-direction. Firing rate decreased for the same eye movements in the OFF-direction. All units showed a steady firing rate proportional to eye position beyond their recruitment threshold. 3. Motoneuronal position (ks) and velocity (rs) sensitivity for spontaneous eye movements were calculated from the slope of the rate-position and rate-velocity linear regression lines, respectively. The averaged ks and rs values of medial rectus motoneurons were higher than those of abducens motoneurons. The differences in motoneuronal sensitivity coupled with structural variations in the lateral versus the medial rectus muscle suggest that symmetric nasal and temporal eye movements are preserved by different motor unit composition. Although the abducens nucleus consists of distinct rostral and caudal subgroups, mean ks and rs values were not significantly different between the two populations. 4. Every abducens and medial rectus motoneuron fired an intense burst of spikes during its corresponding temporal or nasal activation phase of the "eye blink. " This eye movement consisted of a sequential, rather than a synergic, contraction of both vertical and horizontal extraocular muscles. The eye blink could act neither as a protective reflex nor as a goal-directed eye movement because it could not be evoked in response to sensory stimuli. We propose a role for the blink in recentering eye position. 5. Motoneuronal firing rate after ON-directed saccades decreased exponentially before reaching the sustained discharge proportional to the new eye position. Time constants of the exponential decay ranged from 50 to 300 ms. Longer time constants after the saccade were associated with backward drifts of eye position and shorter time constants with onward drifts. These postsaccadic slide signals are suggested to encode the transition of eye position to the new steady level. 6. Motoneurons modulated sinusoidally in response to sinusoidal head rotation in the dark, but for a part of the cycle they went into cutoff, dependent on their eye position recruitment threshold. Eye position (kv) and velocity (rv) sensitivity during vestibular stimulation were measured at frequencies between 1/16 and 2 Hz. Motoneuronal time constants (tau v = rv/kv) decreased on the average by 25% with the frequency of vestibular stimulation. (ABSTRACT TRUNCATED AT 400 WORDS)
Step Time Variability and Pelvis Acceleration Patterns of Younger and Older Adults: Effects of Footwear and Surface Conditions

This study investigated the effects of various shoe features and surfaces on step time variability and pelvis accelerations (RMS) during walking in six younger and 22 older adults. Participants walked at a self-selected speed in five shoe conditions (standard, elevated heel, soft sole, hard sole, and high collar) on two surfaces: level and irregular. Results showed an age-related reduction in step time variability on the irregular surface and increased medio-lateral (ML) acceleration RMS in older people walking on the irregular versus the level surface. When wearing the elevated heel shoes, both young and old participants displayed significant reductions in pelvis ML accelerations, suggesting a compensatory strategy to counteract for lateral instability resulting from heel elevation. Overall, the findings demonstrate that older people are less able than younger ones to meet the stepping adaptability required for negotiating an irregular surface and, as a possible consequence, have more difficulty maintaining frontal plane stability on such a surface.
Enterobius vermicularis and Risk Factors in Healthy Norwegian Children

Background: The prevalence of Enterobius vermicularis in neighboring countries of Norway show large variation. The goal of this study was to investigate the prevalence among Norwegian children and possible risk factors. Methods: The children were participants in “Environmental Triggers of Type 1 Diabetes: the MIDIA study. ” The study involved 2 groups with different genetic risks of type 1 diabetes: A high-risk group carries the Human Leukocyte Antigen genotype conferring the highest risk for type 1 diabetes and a nonhigh-risk group consisting of children without this genotype. Scotch tape samples were collected on 3 consecutive days and examined by light microscopy. Results: A total of 18% (72/395) of children were positive for E. vermicularis. The highest prevalence (34%) was in children 6–11 years of age. Only 2 children were prior known positives. Increased number of siblings was linked to more infections, and there were fewer infections in the children with the high-risk genotype. Conclusion: E. vermicularis is a common parasite in Norwegian children. The likelihood of E. vermicularis infection depends on family size and prevalence increases with age. The reduced number of infections in the children carrying the high-risk genotype for type 1 diabetes is intriguing and should be investigated further.
Critical Factors Influencing the Occurrence of Vibrio cholerae in the Environment of Bangladesh

ABSTRACT The occurrence of outbreaks of cholera in Africa in 1970 and in Latin America in 1991, mainly in coastal communities, and the appearance of the new serotype Vibrio cholerae O139 in India and subsequently in Bangladesh have stimulated efforts to understand environmental factors influencing the growth and geographic distribution of epidemic Vibrio cholerae serotypes. Because of the severity of recent epidemics, cholera is now being considered by some infectious disease investigators as a “reemerging” disease, prompting new work on the ecology of vibrios. Epidemiological and ecological surveillance for cholera has been under way in four rural, geographically separated locations in Bangladesh for the past 4 years, during which both clinical and environmental samples were collected at biweekly intervals. The clinical epidemiology portion of the research has been published (Sack et al., J. Infect. Dis. 187:96-101, 2003). The results of environmental sampling and analysis of the environmental and clinical data have revealed significant correlations of water temperature, water depth, rainfall, conductivity, and copepod counts with the occurrence of cholera toxin-producing bacteria (presumably V. cholerae). The lag periods between increases or decreases in units of factors, such as temperature and salinity, and occurrence of cholera correlate with biological parameters, e.g., plankton population blooms. The new information on the ecology of V. cholerae is proving useful in developing environmental models for the prediction of cholera epidemics.
Does random urine drug testing reduce illicit drug use in chronic pain patients receiving opioids?

BACKGROUND Prescription drug abuse and illicit drug use are common in chronic pain patients. Adherence monitoring with screening tests, and urine drug testing, periodic monitoring with prescription monitoring programs, has become a common practice in recent years. Random drug testing for appropriate use of opioids and use of illicit drugs is often used in pain management practices. Thus, it is expected that random urine drug testing will deter use of illicit drugs, and also improve compliance. OBJECTIVES To study the prevalence of illicit drug use in patients receiving opioids for chronic pain management and to compare the results of illicit drug use with the results from a previous study. DESIGN A prospective, consecutive study. SETTING Interventional pain management practice setting in the United States. METHODS A total of 500 consecutive patients on opioids, considered to be receiving stable doses of opioids supplemental to their interventional techniques, were studied by random drug testing. Testing was performed by rapid drug screen. Results were considered positive if one or more of the monitored illicit drugs including cocaine, marijuana (THC), methamphetamine or amphetamines were present. RESULTS Illicit drug use was evident in 80 patients, or 16%, with marijuana in 11%, cocaine in 5%, and methamphetamine and/or amphetamines in 2%. When compared with previous data, the overall illicit drug use was significantly less. Illicit drug use in elderly patients was absent. CONCLUSION The prevalence of illicit drug abuse in patients with chronic pain receiving opioids continues to be a common occurence. This study showed significant reductions in overall illicit drug use with adherence monitoring combined with random urine drug testing.
Effect of Centella asiatica L (Umbelliferae) on Normal and Dexamethasone-Suppressed Wound Healing in Wistar Albino Rats

Centella asiatica is a reputed medicinal plant used in the treatment of various skin diseases in the Indian system of medicine. The objective of the study presented in this article was to evaluate the wound-healing potential of the ethanolic extract of the plant in both normal and dexamethasone-suppressed wound healing. The study was done on Wistar albino rats using incision, excision, and dead space wounds models. The extract of C asiatica significantly increased the wound breaking strength in incision wound model compared to controls (P < .001). The extract-treated wounds were found to epithelize faster, and the rate of wound contraction was significantly increased as compared to control wounds (P < .001). Wet and dry granulation tissue weights, granulation tissue breaking strength, and hydroxyproline content in a dead space wound model also increased at statistically significant levels as shown. The extract of the leaves had the effect of attenuating the known effects of dexamethasone healing in all wound models (P < .001, P < .05). The results indicated that the leaf extract promotes wound healing significantly and is able to overcome the wound-healing suppressing action of dexamethasone in a rat model. These observations were supported by histology findings.
Mind the Gap

Objective: The aim of this study was to evaluate the effects of key gap (distance between edges of keys) on computer keyboards on typing speed, percentage error, preference, and usability. Background: In Parts 1 and 2 of this series, a small key pitch (center-to-center distance between keys) was found to reduce productivity and usability, but the findings were confounded by gap. In this study, key gap was varied while holding key pitch constant. Method: Participants (N = 25) typed on six keyboards, which differed in gap between keys (1, 3, or 5 mm) and pitch (16 or 17 mm; distance between centers of keys), while typing speed, accuracy, usability, and preference were measured. Results: There was no statistical interaction between gap and pitch. Accuracy was better for keyboards with a gap of 5 mm compared to a 1-mm gap (p = .04). Net typing speed (p = .02), accuracy (p = .002), and most usability measures were better for keyboards with a pitch of 17 mm compared to a 16-mm pitch. Conclusions: The study findings support keyboard designs with a gap between keys of 5 mm over 1 mm and a key pitch of 17 mm over 16 mm. Applications: These findings may influence keyboard standards and design, especially the design of small keyboards used with portable devices, such as tablets and laptops.
Metric-based detection of robot kidnapping

Kidnapping occurs when a robot is unaware that it has not correctly ascertained its position. As a result, the global map may be severely deformed and the robot may be unable to perform its function. This paper presents a metric-based technique for real-time kidnap detection that utilises a set of binary classifiers to identify all kidnapping events during the autonomous operation of a mobile robot. In contrast, existing techniques either solve specific cases of kidnapping, such as elevator motion, without addressing the general case or remove dependence on local pose estimation entirely, an inefficient and computationally expensive approach. Four metrics were evaluated and the optimal thresholds for the most suitable metrics were determined, resulting in a combined detector that has a negligible probability of failing to identify kidnapping events and a low false positive rate for both indoor and outdoor environments. While this paper uses metrics specific to 3D point clouds, the approach can be generalised to other forms of data, including visual, providing that two independent ways of estimating pose are available.
Identification of biological models from single-cell data: A comparison between mixed-effects and moment-based inference

Experimental techniques in biology such as microfluidic devices and time-lapse microscopy allow tracking of the gene expression in single cells over time. So far, few attempts have been made to fully exploit these data for modeling the dynamics of biological networks in cell populations. In this paper we compare two modeling approaches capable to describe cell-to-cell variability: Mixed-Effects (ME) models and the Chemical Master Equation (CME). We discuss how network parameters can be identified from experimental data and use real data of the HOG pathway in yeast to assess model quality. For CME we rely on the identification approach proposed by Zechner et al. (PNAS, 2012), based on moments of the probability distribution involved in the CME. ME and moment-based (MB) inference will be also contrasted in terms of general features and possible uses in biology.
Bortezomib Primes Glioblastoma, Including Glioblastoma Stem Cells, for TRAIL by Increasing tBid Stability and Mitochondrial Apoptosis

Purpose: Searching for novel approaches to sensitize glioblastoma for cell death, we investigated the proteasome inhibitor bortezomib. Experimental Design: The effect of bortezomib on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)–induced apoptosis signaling pathways was analyzed in glioblastoma cell lines, primary glioblastoma cultures, and in an in vivo model. Results: Bortezomib and TRAIL synergistically trigger cell death and reduce colony formation of glioblastoma cells (combination index < 0.1). Investigations into the underlying molecular mechanisms reveal that bortezomib and TRAIL act in concert to cause accumulation of tBid, the active cleavage product of Bid. Also, the stability of TRAIL-derived tBid markedly increases on proteasome inhibition. Notably, knockdown of Bid significantly decreases bortezomib- and TRAIL-mediated cell death. By comparison, silencing of Noxa, which is also upregulated by bortezomib, does not confer protection. Coinciding with tBid accumulation, the activation of Bax/Bak and loss of mitochondrial membrane potential are strongly increased in cotreated cells. Overexpression of Bcl-2 significantly reduces mitochondrial perturbations and cell death, underscoring the functional relevance of the mitochondrial pathway. In addition, bortezomib cooperates with TRAIL to reduce colony formation of glioblastoma cells, showing an effect on long-term survival. Of note, bortezomib profoundly enhances TRAIL-triggered cell death in primary cultured glioblastoma cells and in patient-derived glioblastoma stem cells, underlining the clinical relevance. Importantly, bortezomib cooperates with TRAIL to suppress tumor growth in an in vivo glioblastoma model. Conclusion: These findings provide compelling evidence that the combination of bortezomib and TRAIL presents a promising novel strategy to trigger cell death in glioblastoma, including glioblastoma stem cells, which warrants further investigation. Clin Cancer Res; 17(12); 4019–30. ©2011 AACR.
Cathelicidins: a family of endogenous antimicrobial peptides

The cytoplasmic granules of mammalian neutrophils contain several antimicrobial peptides. Some, like defensins, are fully processed before storage, whereas others are stored as precursors that require additional processing. Cathelicidins are bipartite molecules with an N-terminal cathelin domain and an antimicrobial C-terminal domain. Humans apparently have only one cathelicidin gene. Its product, hCAP-18, is present in the secondary (specific) granules of neutrophils, and its C-terminal antimicrobial peptide, LL-37, is liberated by proteinase 3 coincident with degranulation and secretion. Many nonmyeloid tissues also express hCAP-18, including epididymis, spermatids, keratinocytes, epithelial cells, and various lymphocytes. LL-37 stimulates chemotaxis, acting via the formyl peptide-like receptor-1. The structurally diverse cathelicidin-derived antimicrobial peptides of animals provide interesting models for pharmaceutical development. PR-39, a proline-rich porcine cathelicidin, has shown efficacy in limiting myocardial damage after experimental ischemia in rodent models. Porcine protegrins are in stage III clinical trials to prevent oral mucositis caused by radiation or chemo-therapy.
Lytic Replication of Kaposi's Sarcoma-Associated Herpesvirus Results in the Formation of Multiple Capsid Species: Isolation and Molecular Characterization of A, B, and C Capsids from a Gammaherpesvirus

ABSTRACT Despite the discovery of Epstein-Barr virus more than 35 years ago, a thorough understanding of gammaherpesvirus capsid composition and structure has remained elusive. We approached this problem by purifying capsids from Kaposi's sarcoma-associated herpesvirus (KSHV), the only other known human gammaherpesvirus. The results from our biochemical and imaging analyses demonstrate that KSHV capsids possess a typical herpesvirus icosahedral capsid shell composed of four structural proteins. The hexameric and pentameric capsomers are composed of the major capsid protein (MCP) encoded by open reading frame 25. The heterotrimeric complexes, forming the capsid floor between the hexons and pentons, are each composed of one molecule of ORF62 and two molecules of ORF26. Each of these proteins has significant amino acid sequence homology to capsid proteins in alpha- and betaherpesviruses. In contrast, the fourth protein, ORF65, lacks significant sequence homology to its structural counterparts from the other subfamilies. Nevertheless, this small, basic, and highly antigenic protein decorates the surface of the capsids, as does, for example, the even smaller basic capsid protein VP26 of herpes simplex virus type 1. We have also found that, as with the alpha- and betaherpesviruses, lytic replication of KSHV leads to the formation of at least three capsid species, A, B, and C, with masses of approximately 200, 230, and 300 MDa, respectively. A capsids are empty, B capsids contain an inner array of a fifth structural protein, ORF17.5, and C capsids contain the viral genome.
How can SMEs Leverage Political Ties and Technological Innovation Capability to Acquire Government Assistance in a Transition Economy?

While government assistance can help SMEs remove resource deficiencies, little research has been conducted from the firms' point of view to investigate the factors that can enable firms to win competition for government assistance. This paper attempts to address this gap by building a theoretical model to explore the effects of political ties and technological innovation capability on government assistance as well as the moderating effects of opportunity exploration capability and market-oriented transition. Six hypotheses are proposed and tested using a sample of 169 Chinese SMEs. It was found that both political ties and technological innovation capability have significantly positive effects on government assistance. Furthermore, opportunity exploration capability strengthens the effect of political ties while it weakens that of technological innovation capability. Market-oriented transition strengthens the positive role of technological innovation capability while it does not significantly weaken the role of political ties.
Usage of Social Media by Children and Teenagers: Results of EU KIDS Online II

This chapter presents the results of EU Kids Online II project about the children’s and adolescent’s uses of the social network sites. The results showed both the main differences and similarities regarding this issue both at the European and at the country (i.e. Romania) level. Although at the European level one can notice the emergence of different groups of users, in Romania, the use of the Internet is only at the beginning and has no clear pattern. The individual characteristics in the self-efficacy variant positively vary with the using of SNS and, at the same time, none of the negative individual characteristics predicts the possessing of a profile on a social network. The strongest connection exists between having competences regarding the use of the Internet and owning a profile on a social network. Moreover, the results agree with previous researches that highlight a complex influence of the parental mediation on the social behaviors.
Blood Lactate

SummaryThe blood lactate response to exercise has interested physiologists for over fifty years, but has more recently become as routine a variable to measure in many exercise laboratories as is heart rate. This rising popularity is probably due to: a) the ease of sampling and improved accuracy afforded by recently developed micro-assay methods and/or automated lactate analysers; and b) the predictive and evaluative power associated with the lactate response to exercise. Several studies suggest that the strong relationship between exercise performance and lactate-related variables can be attributed to a reflection by lactate during exercise of not only the functional capacity of the central circulatory apparati to transport oxygen to exercising muscles, but also the peripheral capacity of the musculature to utilise this oxygen. For example, several studies contrast the relationship between V̇O2 max and endurance running performance with that between a lactate variable and the same running performance. In every study, the lactate variable is more highly correlated with performance. Similarly, prescribing training intensity as a function of the lactate concentration elicited by the training may prove to be a means of obtaining a more homogeneous adaptation to training in a group of athletes or subjects than is obtained by setting intensity as a function of maximal heart rate or % V̇O2 max. A review of the recent literature shows that the lactate response to supramaximal exercise is a sensitive indicator of adaptation to ‘sprint training’ and is correlated with supramaximal exercise performance. This review also describes the possible applications of lactate measurements to enhance the rate of recovery from high intensity exercise. Although the lactate response to exercise is reproducible under standardised conditions it can be influenced by the site of blood sampling, ambient temperature, changes in the bodys’s acid-base balance prior to exercise, prior exercise, dietary manipulations, or pharmacological interpretation.