Miracle-dz/UniFAR-FTUs huggingface dataset:
Unpacking childhood experiences of abuse: Can clinicians identify their patients’ History of Abuse?

ABSTRACT Emotional, physical and sexual abuse, have been consistently linked to mental health problems in adults. Previous research found that mental health providers rarely ask their patients about their childhood experiences of abuse. No study to date has examined the convergence of clinicians’ and patients’ reports of childhood abuse. The current study applied a multi-method, multi-informant approach to explore the concordance between patients’ reports of childhood experiences of abuse and clinicians’ identification of their patients’ history of abuse. Assessment of an independent interviewer was included. A convenience sample of clinicians (N = 80) and their patients (N = 170) in mental health clinics in care-as-usual settings participated in the study. To assess the history of abuse clinicians and patients completed the Clinical Data Form, patients additionally completed the Childhood Trauma Questionnaire. Independent interviewer completed the Familial Experiences Interview. Findings show that across all informants, exposure to emotional abuse was most prevalent, followed by physical abuse and least prevalent was sexual abuse. Additionally, clinicians reported lower prevalence of physical and sexual abuse among their patients as compared with the patient and independent interviewer’s reports. Moderate to strong correlations were observed between clinicians, patients and independent interviewer reports of emotional, physical and sexual childhood abuse. Moreover, the severity of the patient’s history of abuse was related to greater accuracy in clinicians’ reports. Clinicians are advised to collect explicit information regarding childhood abuse through interviews or valid measures. Clinicians should pay special attention when assessing patients with moderate severity of childhood abuse since they are frequently under-identified.
Yak (Bos grunniens) bones collagen-derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β-catenin signaling pathway.

BACKGROUND As the world's population is transitioning gradually to an ageing stage, the incidence of osteoporosis increases annually. Yak bone is one of the major components of Tibetan medicine (traditional Chinese medicine, TCM) and its function has mainly been associated with an improvement in bone health, for example, against osteoporosis. However, the functional bioactive ingredients and the underlying mechanisms are still unclear. RESULTS Sequential purification of the yak bone hydrolysates was achieved by ultrafiltration, size exclusion chromatography and semi-preparative reverse phase high-performance liquid chromatography. After then, 35 novel peptides were identified by mass spectrometry analysis, of which peptide GPAGPPGPIGNV (GP-12) displayed the highest osteoblast proliferation-promoting activity with an increase of 42.7% in cell growth. In vitro stability study demonstrated that GP-12 was digested into smaller peptides (GP-9, GV-9, AV-10 and GP-11) after simulated gastrointestinal digestion and Caco-2 cells monolayer model. However, some of them still can be absorbed intact through the Caco-2 cell monolayers via paracellular route (Papp: 5.36±0.34 cm/s). Flow Cytometry results indicated that GP-12 enhanced osteoblastic proliferation by inducing alteration of cell cycle progression both from G0/G1 to S and from S to G2/M phases. Quantitative real-time PCR and western blot results revealed that GP-12 induced osteoblastic proliferation and differentiation with a dose-response manner through the activation of Wnt/β-catenin signaling pathway. CONCLUSION These findings highlighted that such peptides hold promise in discovery of candidates for functional and health promoting foods, which could be potentially used for the treatment of osteoporosis. This article is protected by copyright. All rights reserved.
Emergence of a Thrombospondin Superfamily at the Origin of Metazoans

Abstract Extracellular matrix (ECM) is considered central to the evolution of metazoan multicellularity; however, the repertoire of ECM proteins in nonbilaterians remains unclear. Thrombospondins (TSPs) are known to be well conserved from cnidarians to vertebrates, yet to date have been considered a unique family, principally studied for matricellular functions in vertebrates. Through searches utilizing the highly conserved C-terminal region of TSPs, we identify undisclosed new families of TSP-related proteins in metazoans, designated mega-TSP, sushi-TSP, and poriferan-TSP, each with a distinctive phylogenetic distribution. These proteins share the TSP C-terminal region domain architecture, as determined by domain composition and analysis of molecular models against known structures. Mega-TSPs, the only form identified in ctenophores, are typically >2,700 aa and are also characterized by N-terminal leucine-rich repeats and central cadherin/immunoglobulin domains. In cnidarians, which have a well-defined ECM, Mega-TSP was expressed throughout embryogenesis in Nematostella vectensis, with dynamic endodermal expression in larvae and primary polyps and widespread ectodermal expression in adult Nematostella vectensis and Hydra magnipapillata polyps. Hydra Mega-TSP was also expressed during regeneration and siRNA-silencing of Mega-TSP in Hydra caused specific blockade of head regeneration. Molecular phylogenetic analyses based on the conserved TSP C-terminal region identified each of the TSP-related groups to form clades distinct from the canonical TSPs. We discuss models for the evolution of the newly defined TSP superfamily by gene duplications, radiation, and gene losses from a debut in the last metazoan common ancestor. Together, the data provide new insight into the evolution of ECM and tissue organization in metazoans.
Channelrhodopsin-1 Phosphorylation Changes with Phototactic Behavior and Responds to Physiological Stimuli in Chlamydomonas

Rapid changes in the phosphorylation status of the light receptor ChR1 help the motile green alga Chlamydomonas reinhardtii adapt its phototactic sensitivity to varying environmental conditions. The unicellular alga Chlamydomonas (Chlamydomonas reinhardtii) exhibits oriented movement responses (phototaxis) to light over more than three log units of intensity. Phototaxis thus depends on the cell’s ability to adjust the sensitivity of its photoreceptors to ambient light conditions. In Chlamydomonas, the photoreceptors for phototaxis are the channelrhodopsins (ChR)1 and ChR2; these light-gated cation channels are located in the plasma membrane. Although ChRs are widely used in optogenetic studies, little is known about ChR signaling in algae. We characterized the in vivo phosphorylation of ChR1. Its reversible phosphorylation occurred within seconds as a graded response to changes in the light intensity and ionic composition of the medium and depended on an elevated cytosolic Ca2+ concentration. Changes in the phototactic sign were accompanied by alterations in the phosphorylation status of ChR1. Furthermore, compared with the wild type, a permanently negative phototactic mutant required higher light intensities to evoke ChR1 phosphorylation. C-terminal truncation of ChR1 disturbed its reversible phosphorylation, whereas it was normal in ChR2-knockout and eyespot-assembly mutants. The identification of phosphosites in regions important for ChR1 function points to their potential regulatory role(s). We propose that multiple ChR1 phosphorylation, regulated via a Ca2+-based feedback loop, is an important component in the adaptation of phototactic sensitivity in Chlamydomonas.
A 3D‐printed polycaprolactone/β‐tricalcium phosphate mandibular prosthesis: A pilot animal study

In this study, we assessed the effectiveness of a tonsil‐derived mesenchymal stem cell (TMSC)‐transplanted polycaprolactone/beta‐tricalcium phosphate prosthesis (specifically designed for easier fixing and grafting with a single scaffold) on rabbit mandible osteogenesis.
Pierre D. and the first photographs of Parkinson's disease

School of Pharmacy, University of Reading, Whiteknights, Reading, United Kingdom Royal Veterinary College, London, United Kingdom Department of Neurodegenerative Disease, UCL Institute of Neurology, London, United Kingdom Art and Medicine, New York, New York, USA National Hospital for Neurology and Neurosurgery, London, United Kingdom Guy’s and St. Thomas’ NHS Foundation Trust, St. Thomas’ Hospital, London, United Kingdom
Validity and Reliability of a Standardized Protocol for Assessing the One Repetition Maximum Performance During Overhead Pressing Exercises

Abstract Soriano, MA, García-Ramos, A, Torres-González, A, Castillo-Palencia, J, Ayuso, V, Marín, PJ, and Comfort, P. Validity and reliability of a standardized protocol for assessing the one repetition maximum performance during overhead pressing exercises. J Strength Cond Res 35(11): 2988–2992, 2021—The aim of this study was to determine the validity of performing 3 one repetition maximum (1RM) assessments for the push press (PP), push jerk (PJ), and split jerk (SJ) in sequence in one testing session vs. the criterion method (testing on separate days), while determining the between-session reliability of the combined assessment. Twenty-two well-trained men (n = 22; age: 28.5 + 1.3 years; height: 1.80 + 0.04 m; body mass: 84.9 + 1.9 kg; training experience: 4.27 + 4.08 years) participated in this study. The 1RM was assessed in a sequential order in the same testing session (combined 1RM assessment) for the PP, PJ, and SJ on 2 occasions, to determine between-session reliability. The 1RM for each exercise was also examined on 3 separate sessions to compare the results against the combined method. A high reliability, low variability, and low measurement error were evident for the PP (intraclass correlation coefficient [ICC] = 0.960; coefficient of variation [CV] = 1.8%; smallest detectable difference [SDD] = 7.1%), PJ (ICC = 0.978; CV = 1.5%; SDD = 5.4%) and SJ (ICC = 0.987; CV = 0.8%; SDD = 4.6%). In addition, there were no significant (p > 0.05) or meaningful (η2 ≤0.001) differences between the single and combined assessments. The high reliability and validity of the combined assessment suggest that practitioners and researchers may simplify the testing procedure by assessing the 1RM during the 3 main overhead pressing exercises in a single testing session.
Six Key Enablers for Machine Type Communication in 6G

While 5G is being rolled out in different parts of the globe, few research groups around the world $-$ such as the Finnish 6G Flagship program $-$ have already started posing the question: \textit{What will 6G be? } The 6G vision is a data-driven society, enabled by near instant unlimited wireless connectivity. Driven by impetus to provide vertical-specific wireless network solutions, machine type communication encompassing both its mission critical and massive connectivity aspects is foreseen to be an important cornerstone of 6G development. This article presents an over-arching vision for machine type communication in 6G. In this regard, some relevant performance indicators are first anticipated, followed by a presentation of six key enabling technologies.
A Recognition Method for Rice Plant Diseases and Pests Video Detection Based on Deep Convolutional Neural Network

Increasing grain production is essential to those areas where food is scarce. Increasing grain production by controlling crop diseases and pests in time should be effective. To construct video detection system for plant diseases and pests, and to build a real-time crop diseases and pests video detection system in the future, a deep learning-based video detection architecture with a custom backbone was proposed for detecting plant diseases and pests in videos. We first transformed the video into still frame, then sent the frame to the still-image detector for detection, and finally synthesized the frames into video. In the still-image detector, we used faster-RCNN as the framework. We used image-training models to detect relatively blurry videos. Additionally, a set of video-based evaluation metrics based on a machine learning classifier was proposed, which reflected the quality of video detection effectively in the experiments. Experiments showed that our system with the custom backbone was more suitable for detection of the untrained rice videos than VGG16, ResNet-50, ResNet-101 backbone system and YOLOv3 with our experimental environment.
Improved Complex Skill Acquisition by Immersive Virtual Reality Training: A Randomized Controlled Trial.

BACKGROUND There has been limited literature on immersive virtual reality (VR) simulation in orthopaedic education. The purpose of this multicenter, blinded, randomized controlled trial was to determine the validity and efficacy of immersive VR training in orthopaedic resident education. METHODS Nineteen senior orthopaedic residents (resident group) and 7 consultant shoulder arthroplasty surgeons (expert group) participated in the trial comparing immersive VR with traditional learning using a technical journal article as a control. The examined task focused on achieving optimal glenoid exposure. Participants completed demographic questionnaires, knowledge tests, and a glenoid exposure on fresh-frozen cadavers while being examined by blinded shoulder arthroplasty surgeons. Training superiority was determined by the outcome measures of the Objective Structured Assessment of Technical Skills (OSATS) score, a developed laboratory metric, verbal answers, and time to task completion. RESULTS Immersive VR had greater realism and was superior in teaching glenoid exposure than the control (p = 0.01). The expert group outperformed the resident group on knowledge testing (p = 0.04). The immersive VR group completed the learning activity and knowledge tests significantly faster (p < 0.001) at a mean time (and standard deviation) of 11 ± 3 minutes than the control group at 20 ± 4 minutes, performing 3 to 5 VR repeats for a reduction in learning time of 570%. The immersive VR group completed the glenoid exposure significantly faster (p = 0.04) at a mean time of 14 ± 7 minutes than the control group at 21 ± 6 minutes, with superior OSATS instrument handling scores (p = 0.03). The immersive VR group scored equivalently in surprise verbal scores (p = 0.85) and written knowledge scores (p = 1.0). CONCLUSIONS Immersive VR demonstrated substantially improved translational technical and nontechnical skills acquisition over traditional learning in senior orthopaedic residents. Additionally, the results demonstrate the face, content, construct, and transfer validity for immersive VR. CLINICAL RELEVANCE This adequately powered, randomized controlled trial demonstrated how an immersive VR system can efficiently (570%) teach a complex surgical procedure and also demonstrate improved translational skill and knowledge acquisition when compared with a traditional learning method.
Perceptual inference employs intrinsic alpha frequency to resolve perceptual ambiguity

The brain uses its intrinsic dynamics to actively predict observed sensory inputs, especially under perceptual ambiguity. However, it remains unclear how this inference process is neurally implemented in biasing perception of ambiguous inputs towards the predicted percepts. Using electroencephalography and intracranial recordings, we first show that the alpha-band frequency defines a unified time window for perceptual grouping across both space and time: information segments, either spatially or temporally segregated, will be integrated if they fall within the same alpha cycle. Moreover, predictions employ this prior knowledge on intrinsic alpha frequency to shift perceptual inference towards the most possibly observed percepts. Multivariate decoding analysis showed that perceptual inference, based on variance in prestimulus alpha frequency (PAF), biases post-stimulus neural representations by inducing preactivation of the predicted percepts. fMRI results additionally showed that prestimulus activity and intrinsic organization status in the frontoparietal attentional network predict perceptual outcomes, probably by modulating occipitoparietal PAFs.
Perceptions of the Use of Alcohol and Drugs after Sudden Bereavement by Unnatural Causes: Analysis of Online Qualitative Data

Bereavement is associated with an increased risk of psychiatric morbidity and all-cause mortality, particularly in younger people and after unnatural deaths. Substance misuse is implicated but little research has investigated patterns of drug or alcohol use after bereavement. We used a national online survey to collect qualitative data describing whether and how substance use changes after sudden bereavement. We conducted thematic analysis of free-text responses to a question probing use of alcohol and drugs after the sudden unnatural (non-suicide) death of a family member or a close friend. We analysed data from 243 adults in British Higher Education Institutions aged 18–40, identifying two main themes describing post-bereavement alcohol or drug use: (1) sense of control over use of drugs or alcohol (loss of control versus self-discipline), (2) harnessing the specific effects of drugs or alcohol. Across themes we identified age patterning in relation to substance misuse as a form of rebellion among those bereaved in childhood, and gender patterning in relation to men using alcohol to help express their emotions. The limitations of our sampling mean that these findings may not be generalizable from highly-educated settings to young people in the general population. Our findings describe how some young bereaved adults use drugs and alcohol to help them cope with traumatic loss, and suggest how clinicians might respond to any difficulties controlling substance use.
Computational analysis of functional single nucleotide polymorphisms associated with SLC26A4 gene

Single Nucleotide Polymorphisms (SNPs) are the most common candidate mutations in human beings that play a vital role in the genetic basis of certain diseases. Previous studies revealed that Solute Carrier Family 26 Member 4 (SLC26A4) being an essential gene of the multi-faceted transporter family SLC26 facilitates reflexive movement of Iodide into follicular lumen through apical membrane of thyrocyte. SLC26A4 gene encodes Pendred protein, a membrane glycoprotein, highly hydrophobic in nature, present at the apical membrane of thyrocyte functioning as transporter of iodide for thyroid cells. A minor genetic variation in SLC26A4 can cause Pendred syndrome, a syndrome associated with thyroid glands and deafness. In this study, we performed in-silico analysis of 674 missense SNPs of SLC26A4 using different computational platforms. The bunch of tools including SNPNEXUS, SNAP-2, PhD-SNP, SNPs&GO, I-Mutant, ConSurf, and ModPred were used to predict 23 highly confident damaging and disease causing nsSNPs (G209V, G197R, L458P, S427P, Q101P, W472R, N392Y, V359E, R409C, Q235R, R409P, G139V, G497S, H723R, D87G, Y127H, F667C, G334A, G95R, S427C, R291W, Q383H and E384G) that could potentially alter the SLC26A4 gene. Moreover, protein structure prediction, protein-ligand docking and Molecular Dynamics simulation were performed to confirm the impact of two evident alterations (Y127H and G334A) on the protein structure and function.
Temporal information loss in the macaque early visual system

Stimuli that modulate neuronal activity are not always detectable, indicating a loss of information between the modulated neurons and perception. To identify where in the macaque visual system information about periodic light modulations is lost, signal-to-noise ratios were compared across simulated cone photoreceptors, lateral geniculate nucleus (LGN) neurons, and perceptual judgements. Stimuli were drifting, threshold-contrast Gabor patterns on a photopic background. The sensitivity of LGN neurons, extrapolated to populations, was similar to the monkeys' at low temporal frequencies. At high temporal frequencies, LGN sensitivity exceeded the monkeys' and approached the upper bound set by cone photocurrents. These results confirm a loss of high-frequency information downstream of the LGN. However, this loss accounted for only about 5% of the total. Phototransduction accounted for essentially all of the rest. Together, these results show that low temporal frequency information is lost primarily between the cones and the LGN, whereas high-frequency information is lost primarily within the cones, with a small additional loss downstream of the LGN.
Cesarean delivery: Trend in indications over three decades within a major city hospital network

The cesarean delivery rate has been increasing globally in recent decades. The reasons for this are complex and subject to ongoing debate. Investigation of the indications for cesarean delivery and how these have changed over an extended period of time could provide insight into the reasons for changing obstetric practice. Our objective was to explore contributing factors to the increasing rate of cesarean delivery by examining the incidence of and indications for cesarean delivery over the past three decades at our institutions.
Uncovering the activities, biological roles, and regulation of bacterial cell wall hydrolases and tailoring enzymes

Bacteria account for 1000-fold more biomass than humans. They vary widely in shape and size. The morphological diversity of bacteria is due largely to the different peptidoglycan-based cell wall structures that encase bacterial cells. Although the basic structure of peptidoglycan is highly conserved, consisting of long glycan strands that are cross-linked by short peptide chains, the mature cell wall is chemically diverse. Peptidoglycan hydrolases and cell wall–tailoring enzymes that regulate glycan strand length, the degree of cross-linking, and the addition of other modifications to peptidoglycan are central in determining the final architecture of the bacterial cell wall. Historically, it has been difficult to biochemically characterize these enzymes that act on peptidoglycan because suitable peptidoglycan substrates were inaccessible. In this review, we discuss fundamental aspects of bacterial cell wall synthesis, describe the regulation and diverse biochemical and functional activities of peptidoglycan hydrolases, and highlight recently developed methods to make and label defined peptidoglycan substrates. We also review how access to these substrates has now enabled biochemical studies that deepen our understanding of how bacterial cell wall enzymes cooperate to build a mature cell wall. Such improved understanding is critical to the development of new antibiotics that disrupt cell wall biogenesis, a process essential to the survival of bacteria.