Miracle-dz/UniFAR-FTUs huggingface dataset:
Escherichia coli Ekspresyon Plazmiti pTolT’nin Nokta Mutasyonu Yöntemiyle Moleküler Modifikasyonu

Bakteriler rekombinant protein uretimi icin kolay kultur edilmeleri, basit yapilari ve genetik manipulasyonlara acik olmalari nedeniyle en cok tercih edilen konakcilardandir. Ozellikle Escherichia coli en yaygin olarak kullanilan prokaryotik konakci olarak pek cok arastirmada karsimiza cikmaktadir. Tum bu avantajlarinin yaninda, yabanci proteinlerin yuksek miktardaki uretimleri, Escherichia coli ’de inkluzyon cisimciklerinin olusmasina veya toksik etkilere neden olabilmektedir. Bu problemlerin asilmasi amaciyla gelistirilen pTolT ekspresyon vektoru heterolog proteinlerin Escherichia coli periplazmik bolge proteini TolAIII ile birlikte uretilmesine imkân saglamaktadir. Bununla birlikte TolAIII proteininde 31 ve 56. pozisyonda bulunan sisteinler bu sistemle uretilen diger proteinler ile istenmeyen disulfit kopruleri kurarak rekombinant proteinin aktivitesi uzerine etki etmektedir. Bu calismanin amaci TolAIII proteininde bulunan sisteinlerin kaldirilarak yerine serin amino asitlerinin getirilmesidir. In vitro nokta mutasyonu teknigi, gen klonlama ve ekspresyon stratejilerinin duzenlenmesi amaciyla vektor DNA dizilerinin modifiye edilmesinde, protein yapi ve fonksiyon calismalarinda ve genetik arastirmalarda siklikla kullanilan gelismis bir tekniktir. Bu teknikte mutajenik primerler vasitasiyla polimeraz zincir reaksiyonu (PZR) islemi ile kalip plazmid DNA’nin bircok yeni kopyasini meydana getirilir. Sunulan calismada QuikChange site-directed mutagenesis metoduyla TolAIII gen dizisinde C31S ve C56S mutasyonlari gerceklestirilmistir. DNA sekans analizi sonuclari ile teyit edilen mutasyonun ardindan elde edilen yeni plazmid pTolT Delta olarak adlandirilmistir. Boylece bu calisma ile gelistirilen yeni ekspresyon sistemi ile uretilecek rekombinant proteinlerin dogal uc boyutlu konformasyonlarini kazanmalari daha kolay olacaktir.
Molecular and cellular heterogeneity of gastric cancer explained by methylation-driven key regulators

Gastric cancer (GC) is a heterogeneous disease of diverse genetic, genomic, and epigenetic alterations. Tumor microenvironment (TME) also contributes to the heterogeneity of GC. To investigate GC heterogeneity, we developed an Integrative Sequential Causality Test (ISCT) to identify key regulators of GC by integrating DNA methylation, copy number variation, and transcriptomic data. Applying ISCT to three GC cohorts containing methylation, CNV and transcriptomic data, 11 common methylation-driven key regulators (ADHFE1, CDO1, CRYAB, FSTL1, GPT, PKP3, PTPRCAP, RAB25, RHOH, SFN, and SORD) were identified. Based on these 11 genes, gastric tumors were clustered into 3 clusters which were associated with known molecular subtypes, Lauren classification, tumor stage, and patient survival, suggesting significance of the methylation-driven key regulators in molecular and histological heterogeneity of GC. We further showed that chemotherapy benefit was different in the 3 GC clusters and varied depending on the tumor stage. Both immune/stromal proportions in TME and tumor cell genomic variations contributed to expression variations of the 11 methylation-driven key regulators and to the GC heterogeneity.
Investigation on Dynamic Response of Grid-Tied VSC During Electromechanical Oscillations of Power Systems

This study focuses on the dynamics of a grid-tied voltage source converter (GVSC) during electromechanical oscillations. A small-signal model with GVSC port variables (DC voltage and AC power) as the outputs and a terminal voltage vector as the input is derived to reveal the passive response of the GVSC on the basis of the power equation in the d–q coordinate system. An input–output transfer function matrix is constructed according to the proposed model. The frequency response of this matrix in the electromechanical bandwidth is described to reflect the dynamic behavior of the GVSC. The effects of the operation parameters, i.e., the grid strength, reference value of the control system, and grid voltage, on the dynamic behavior of the GVSC in the electromechanical bandwidth, are investigated using frequency domain sensitivity. Analysis results show that the GVSC generates responses with respect to the electromechanical mode. These responses have different sensitivities to the operation parameters. The IEEE 10-machine power system simulation is performed, and the power hardware-in-the-loop platform with the GVSC was applied to validate the analysis.
Pulp, Leaf, Peel and Seed of Avocado Fruit: A Review of Bioactive Compounds and Healthy Benefits

ABSTRACT Avocado (Persea americana Mill) is a native American fruit. Its industrial processing generates a large number of wastes (leaves, peels, and seeds). These wastes are a source of bioactive compounds which have been attributed biological activities. We aim to compile scientific research on bioactive compounds of avocado pulp and wastes and their potential biological properties. Main bioactive compounds identified in pulp and wastes are polyphenols, carotenoids, tocopherols, and phytosterols. Thus, wastes extracts have reported numerous biological activities, e.g., antimicrobial, anti–inflammatory, anticancer, antidiabetic, antihypertensive. Therefore, potential applications in food and pharmaceutical industries can be issued.
ANALYSING THE PHYLOGENETIC STATUS OF SEVEN SPECIES OF THE SUPERFAMILY COREOIDEA (INSECTA: HEMIPETRA: HETEROPTERA) FROM SOUTH INDIA

Coreoidea is one of the Superfamily in Heteropteran suborder of class Insecta. Most of the Coreoid bugs are pests since they cause damage to many economically important crops. In the present work, eight specimens of seven species belonging to the three families of Superfamily Coreoidea from Kerala have been presented. The Mitochondrial Cytochrome Oxidase 1 sequences of these specimens are compared with the world database. One of among them is a new submission from the world, while others are pioneer submissions from Kerala, India. This study proves that CO1 barcoding is an excellent molecular tool for species level identification of unknown taxa.
Observational learning in food choices: The effect of product familiarity and closeness of peers

Funding information University of Copenhagen, in the KU2016 Plants for a Changing World project; Center for Macooecology, Evolution and Climate, Grant/Award Number: DNRF 96 Abstract The behavior among peers may be interpreted as useful information in individuals' decision processes, although such observational learning is typically not accommodated in consume choice models. This study incorporates information about other consumers' behavior in a choice experiment to evaluate if it affects choice probabilities. Market share is used as a proxy to signal that a product is chosen by many of the individual's peers. Although the effect is associated with diversity, there is a segment of individuals that perceive high market shares to carry relevant information for their food purchasing decision. Respondents who expect to hold similar or more positive preferences towards an unfamiliar food attribute relative to their peers are positively affected by a high market share. Taking information signals from peers' behavior into account may improve understanding of consumer behavior and improve our ability to predict the reception of new food aspects. [EconLit Citations: Q18; D12; D83].
İnsulin Duyarlılaştırıcı İlaçlar Metformin ve Roziglitazonun Polikistik Over Sendromunda Etkinliklerinin Karşılaştırılması

Amac: Polikistik over hastaligi, dogurganlik cagindaki kadinlarda en sik gorulen endokrin hastaligidir. Insulin direnci bu hastalik tablosunun ana patolojilerden birisidir. Insulin duyarlilastirici ajanlar ise bu sendromun tedavisinde klinik ve biyokimyasal acidan basari elde edilmesi amaciyla kullanilmasina karsin, bu ajanlarin PKOS’de kullanimini destekleyen direk veriler netlik kazanmamistir. PKOS’da metformin ve roziglitazon tedavilerinin biyokimyasal ve klinik parametreler uzerine etkilerinin degerlendirmesi ve iki ilacin kiyaslanmasi. Metot: Calismaya PKOS tanisi almis 24 kadin dahil edildi. Tum katilimcilarin bazal antropometrik (VKI, bel cevresi) olcumleri kaydedildikten sonra adetin 2 ile 6. gunleri arasinda gece acligini takiben aclik palzma glikozu, insulin, LH, FSH, DHEAS, total and serbest testosterone duzeyleri olculdu. Insulin direnci gostergesi olarak HOMA-IR (Homeostasis model assessment score-insulin resistance) hesaplandi. Modifiye Ferriman Gallwey yontemi ile hirsutizm skorlamasi yapildi. Sonrasinda tum kastilimcilar metformin (1700mg/gun) veya roziglitazon (4mg/gun) tedavilerine rastgele ayrildilar. Yirmi dort haftalik tedavi suresi sonunda bazal degerlendirmesi yapilan tum olcutler tekrar degerlendirildi. Bazal ve son nokta olcumleri tum grupta ve tedavi gruplarinda ayri ayri karsilastirildi. Bulgular: Bazal olcumler icinde insulin ve serbest testosteron duzeyleri arasinda istatistiksel olarak anlamli bir iliski saptandi. Insulin duyarlilastirici ilaclar ile tedavi sonrasinda tum grup degerlendirildiginde, serbest testosteron duzeyleri, hirsutizm skorlari ve mastruel siklus sikliginda istatistiksel olarak anlamli bicimde duzelme gozlenirken (sirasiyla, P=.004, P=.049 ve P=.001), aclik insulin duzeylerinde ve HOMA-IR olcumlerinde duzelme ise istatistiksel olarak anlamli degildi. Tedavi gruplari ayri ayri analiz edildiginde, metformin grubu hirsutizm skorlarinda istatistiksel olarak anlamli olmayan bir duzelme gozlenirken diger olcutlerde benzer sonuclar elde edildi. Sonuc: Bu bulgular, metformin ve roziglitazon tedavilerinin PKOS’nin klinik ve biyokimyasal bulgularinda iyilesme sagladigini gostermektedir. Bu iki ilac etkinligi acisindan biri digerine ustun olmadigi soylenebilir. Ancak daha cok denek iceren calismalara ihtiyac vardir.
Role of Exopolysaccharide in Pantoea agglomerans Interactions with Bacteriophages

Fire blight is the common name given to a disease caused by phytopathogen, Erwinia amylovora. This pathogen infects plants belonging to the rosaceous family, particularly the commercially grown apple and pear cultivars in North America. Agriculture and Agri-Food Canada Research team has focused on the use of bacteriophages as biological control agents for the control of fire blight. Bacteriophage persistence in the orchard environment can be affected by various environmental factors such as exposure to ultraviolet light and dry conditions. In order to improve phage viability during application, a carrier-phage system was developed that uses a non-pathogenic epiphytic bacterium, named “the carrier”, that protects the bacteriophages during processing and field applications. The bacterium used is Pantoea agglomerans, which delivers and propagates the phages to the open blossoms, prior to the arrival of E. amylovora. In addition, the carrier alone may act as a biological control agent on the blossom surface, competing for space and producing antibiotics targeting E. amylovora. The aim of this study was study the interaction between the phage and the carrier bacterium, P. agglomerans. The exopolysaccharide (EPS) layer, is the first cellular component which bacteriophages encounter during the infection of P. agglomerans. PCR was conducted and it confirmed that 6 genes involved in EPS biosynthesis were present within the four different isolates of P. agglomerans. A CRISPR/Cas9 knockout and lambda red recombineering protocol was used to construct EPS deficient P. agglomerans mutants. The deficient populations were confirmed using colony appearance, PCR and Sangar sequencing. A quantitative PCR was conducted to quantify and compare the different bacteriophage populations between the wild-type and deficient EPS mutants. The EPS mutants were found to have shown a significantly decreased or complete lack of growth in both Myoviridae and Podoviridae infections. MSc Thesis: P. agglomerans phage interaction P. Zaprzala
Heteronymous muscle responses to noisy achilles tendon vibration during standing

Background and Aim: Primary (Ia) afferents have been shown to influence both homonymous (parent muscle) and heteronymous (muscles other than the parent muscle) motoneurons. Specifically, the Ia muscle spindle afferents of Soleus have been shown to influence motoneurons of muscles acting at the knee and hip joints through both monosynaptic and polysynaptic pathways in humans [1,2]. The purpose of this study was to investigate the heteronymous (thigh and back muscle) responses to noisy Achilles tendon vibration during quiet standing. Methods: Noisy vibration (10-115 Hz) was applied to right Achilles tendon to investigate the frequency characteristics of the heteronymous responses in bilateral Semitendinosus, Vastus Lateralis, and Erector Spinae muscles. Participants (n=12; 5 Male) maintained standing balance for a series of two minute trials. Coherence was estimated between the tendon probe acceleration (input signal) and the rectified EMG (output signal) of each muscle. Results: We found significant coherence between the tendon probe acceleration and the EMG of ipsilateral Soleus (across a 10-70 Hz bandwidth), Semitendinosus (10-60 Hz), and Vastus Lateralis (10-60 Hz). Responses were observed bilaterally in Erector Spinae (10-50 Hz), with both ipsilateral and contralateral muscles showing similar coherence. Conclusions: Results from this study provide evidence that muscle spindle feedback from the triceps surae influences the activity of proximal muscles that are important for the control of standing balance. [1] Pierrot-Deseilligny et al., Exp. Brain Res. 42, 337-350, 1981; [2] Meunier et al., Exp. Brain Res. 96, 534-544, 1993;Acknowledgments: Funded by NSERC
Seroprevalence of hepatitis B and C virus in patients who undergo hemodialysis in Antalya province, Turkey

Abstract Background Chronic hepatitis B (HBV) and C virus (HCV) infections are important causes of morbidity and mortality in patients who undergo hemodialysis (HD). Objectives To define seroprevalence of HBV and HCV in patients who underwent HD in Antalya province, Turkey. Methods We included 1347 patients with end-stage renal failure who underwent HD at one of the 23 centers in Antalya province from January 01 to March 31, 2014 in this retrospective cross-sectional study. Hepatitis B surface antigen (HBsAg) and anti-HCV seropositivity were assessed clinically using a third-generation enzyme-linked immunosorbent assay. HBV DNA and HCV RNA were determined in HBsAg positive and anti- HCV positive HD patients respectively. Results Of the patients included, 805 (59.8%) were male. Mean age (± standard deviation) of the patients was 53.9 ± 17.0 (range 17–89) years. The sera of 2.4% patients was positive for HBsAg, and the sera of 5.5% of the patients was positive for anti-HCV. The sera of 56% of patients positive for HBsAg was also positive for HBV DNA, and the sera of 43% of patients positive for anti-HCV was also positive for HCV RNA. Coexistence of HBsAg and anti-HCV was 1.02%. Conclusions The present study showed that the prevalence of chronic HBV and HCV infection in patients who underwent HD in Antalya province was moderate-to-low for Turkey. Compliance of HD centers with infection control rules, isolation of HBsAg positive patients, isolation of the equipment used for HBV susceptible patients, and active surveillance of the HD patients may have resulted in lower prevalence rates.
Utilization of Rice Flour, Awak Banana, Red Beans, Herbal Chicken Liver and Feet to Enrich Micronutrients Content of Complementary Food

The prevalence of stunting in a toddler is still quite high. The dominant cause is related to the low acquisition of complementary foods (CF) during the second year, especially in the fulfillment of micronutrients. Therefore, the study of the use of potential food is needed to enrich the nutrient content, especially micronutrients in CF formulations, to overcome the problem of stunting in the first thousand days of children life. This paper discusses the use of rice flour (R), awak banana (Bn), red beans (B), herbal chicken livers (L) and feet (F) in CF formulation. Formulation of CF was carried out through experimental studies using a complete randomized design with three formulas, namely RBnBLF, RBnBF, and RBnBL. The nutrient content was carried out by sending samples to Laboratory in Jogjakarta. The results showed that the third formula CF has a protein content and fat almost the same i.e. respectively 14% and 11%, carbohydrate content 56.1-61.18% and energy 380.56-397.96%, vitamin A as much as 319.99-439. 85μg, vitamin C 39.11-54,51mg, calcium 0.51-1.26%, phosphorus 0.37-0.59%, iron 19.69-24,07mg, and Zinc 5.46. 0mg. It was concluded that the use of several potential foods can enrich the micronutrient content of CF
Leadership Practices of Supervisory Employees: An Exploration of Current Practices at a Southeastern Veterans Affairs Medical Center

As the nation’s healthcare system moves through the 21 st century, unprecedented changes are occurring on both a local and global stage. Healthcare organizations are faced with creating and implementing leadership strategies to enhance the overall patient experience. When coupled with the need to ensure increased nurse job satisfaction, improved cost-effectiveness and healthy organizational cultures with fiscally sound budgets, improvement work has led efforts to ensure effective leadership techniques are used across an agency. This task may be viewed as commonplace for some organizations, while others may perceive this process as a complete paradigm shift from historical practices related to leadership style, behaviors and performance. A successful transition during this time of unprecedented change may depend on an organization’s ability to accept and implement the tenets of transformational leadership. Empirical research illustrates that transformational leadership empowers staff, increases job satisfaction and facilitates cost-effectiveness while constructing an environment conducive to the development of a supportive organizational culture. In an effort to ascertain the current state of leadership at a southeastern Veterans Affair Medical Center, this research study explored the self-reporting leadership practices of all supervisory staff employed at the facility. As a means of measurement, the Leadership Practices Inventory (LPI) was coupled by demographic questionnaire developed by the researcher. Both measurement tools were used to collect the data.
Land Use Change Impacts on Water Erosion in Rwanda

Rwanda has experienced accelerated soil erosion as a result of unsustainable human activities and changes in land use. Therefore, this study aimed at applying the RUSLE (Revised Universal Soil Loss Equation) model using GIS (Geographical Information System) and remote sensing to assess water erosion in Rwanda, focusing on the erosion-prone lands for the time span 2000 to 2015. The estimated mean annual soil losses were 48.6 t ha−1 y−1 and 39.2 t ha−1 y−1 in 2000 and 2015, respectively, resulting in total nationwide losses of approximately 110 and 89 million tons. Over the 15 years, 34.6% of the total area of evaluated LULC (land use/land cover) types have undergone changes. The highest mean soil loss of 91.6 t ha−1 y−1 occurred in the area changing from grassland to forestland (0.5%) while a mean soil loss of 10.0 t ha−1 y−1 was observed for grassland converting to cropland (4.4%). An attempt has been made to identify the embedded driving forces of soil erosion in Rwanda. As a result, we found that mean soil loss for Rwanda’s districts in 2015 was significantly correlated with poverty (r = 0.45, p = 0.013), increased use of chemical fertilizers (r = 0.77, p = 0.005), and especially was related to extreme poverty (r = 0.77, p = 0.000). The soil conservation scenario analysis for Rwanda’s cropland in 2015 revealed that terracing could reduce the soil loss by 24.8% (from 14.6 t ha−1 y−1 to 11.7 t ha−1 y−1). Most importantly, the study suggests that (1) terracing integrated with mulching and cover crops could effectively control water erosion while ameliorating soil quality and fertility, and (2) reforestation schemes targeting the rapid-growing tree species are therefore recommended as an important feature for erosion control in the study area.
An ethnographic study to understand the patient and nurse experience of rounding

Background Nurse rounding has been widely practiced in the UK since 2012, transferred from the US as a nursing intervention which positively impacts on patient outcomes. Current evidence highlighted a dominance of quantitative studies tentatively linking rounding to impact measures of reduced falls, pressure sore incidence and reduced use of call bells. Outcome measures fail to elicit an understanding of what rounding means from the perspective of the patient or the nurse, moreover applied research evidence in the UK was limited. This study was designed to understand how the practice of rounding impacts on patient experience and nursing care in the NHS. Method Ethnographic methodology was used to see, listen and talk about rounding with nurses and patients. Data collection involved participant observations (38), nurse (34) and patient (34) interviews alongside the analysis of documentary data across two NHS in-patient wards. Findings Findings exposed a culture of rounding practice different from the process described in the literature, encapsulated within four themes: Presence, Actioning Care, Playing the Routine, and Engagement. Reduced falls rates and pressure ulcer prevalence were flawed outcomes of rounding practice, rounding had both social and clinical outcomes depending on the person delivering the round, and patients valued the regular presence of the rounder particularly the nurse in charge. The rounding model generated defined the outcomes of nursing rounding. Conclusion The new knowledge identified rounding practice in its current form was in danger of becoming a tick box exercise with limited impact on patient experience and patient safety. However, recommendations identify opportunities exist for nursing to develop rounding as a mode of care delivery or as a way of offering social presence and engagement to patients. The constituents and constructs of the rounding process need to be further understood to find the true value of the practice to nursing.