Miracle-dz/UniFAR-FTUs huggingface dataset:
Interaction between PAR-3 and the aPKC–PAR-6 complex is indispensable for apical domain development of epithelial cells

The evolutionarily conserved polarity proteins PAR-3, atypical protein kinase C (aPKC) and PAR-6 critically regulate the apical membrane development required for epithelial organ development. However, the molecular mechanisms underlying their roles remain to be clarified. We demonstrate that PAR-3 knockdown in MDCK cells retards apical protein delivery to the plasma membrane, and eventually leads to mislocalized apical domain formation at intercellular regions in both two-dimensional and three-dimensional culture systems. The defects in PAR-3 knockdown cells are efficiently rescued by wild-type PAR-3, but not by a point mutant (S827/829A) that lacks the ability to interact with aPKC, indicating that formation of the PAR-3–aPKC–PAR-6 complex is essential for apical membrane development. This is in sharp contrast with tight junction maturation, which does not necessarily depend on the aPKC–PAR-3 interaction, and indicates that the two fundamental processes essential for epithelial polarity are differentially regulated by these polarity proteins. Importantly, highly depolarized cells accumulate aPKC and PAR-6, but not PAR-3, on apical protein-containing vacuoles, which become targeted to PAR-3-positive primordial cell-cell contact sites during the initial stage of the repolarization process. Therefore, formation of the PAR-3–aPKC–PAR-6 complex might be required for targeting of not only the aPKC–PAR-6 complex but also of apical protein carrier vesicles to primordial junction structures.
Severe myoclonic epilepsy of infancy (Dravet syndrome): Clinical and genetic features of nine Turkish patients

Purpose: Mutations of the α-1 subunit sodium channel gene (SCN1A) cause severe myoclonic epilepsy of infancy (SMEI). To date, over 300 mutations related to SMEI have been described. In the present study, we report new SCN1A mutations and the clinical features of SMEI cases. Materials and Methods: We studied the clinical and genetic features of nine patients diagnosed with SMEI at the Pediatric Neurology Department of Istanbul Medical Faculty. Results: Five patients had nonsense mutations, two had missense mutations, one had a splice site mutation and one had a deletion mutation of the SCN1A gene. Mutations at c.3705+5G splice site, p.trip153X nonsense mutation and deletion at c. 2416_2946 have not been previously described. The seizures started following whole cell pertussis vaccination in all patients. The seizures ceased in one patient and continued in the other eight patients. Developmental regression was severe in three patients, with frequent status epilepticus. The type of mutation was not predictive for the severity of the disease. Two of the three patients with severe regression had nonsense and missense mutations. Conclusions: Dravet syndrome can be result of several different types of mutation in SCN1A gene. Onset of the seizures after pertussis vaccination is an important clue for the diagnosis and neuro- developmental delay should be expected in all patients.
FlexCVA: A Continuously Variable Actuator for Active Orthotics

Active orthotic devices require actuators with high torque, a free movement mode, high efficiency, and small size and weight. The FlexCVA is a new type of actuator that addresses these requirements in a unique way. It provides continuously varying torque through the use of two belts alternately deflected by cams. The belts alternate in supplying output torque with one belt pulling the output while the slack is removed from the other belt. The drive ratio is automatically adjusted in response to the load by varying the deflection distance of the belts. The resulting actuator prototype has been demonstrated to supply up to 34 Nm of output torque, and its free movement mode allows it to be backdriven at speeds greater than 400 deg/sec
Effect of Guar Gum on Body Weight and Serum Lipids in Hypercholesterolemic Females

: The effect of guar gum (15 g/day) on serum lipids and body weight of middle-aged hypercholesterolemic females was studied in a double-blind controlled trial. No consistent changes were observed in serum cholesterol, triglycerides or HDL cholesterol in patients taking guar gum, placebo or no medication at all. A highly significant decrease in body weight (62.9 +/- 2.1 vs. 60.4 +/- 2.2 kg, p < 0.0005, paired comparison) was seen in subjects receiving guar gum, whereas body weight remained constant in the other two groups. It is concluded that the daily ingestion of 15 g of guar gum results in a permanent weight loss, but does not influence serum lipids in females with hypercholesterolemia.
Penalty Function Method for Peer Selection over Wireless Mesh Network

Appropriate peer selection from the discovered file holders plays a vital role for peer-to-peer (P2P) file sharing over wireless mesh networks (WMNs). When peers choose their own utility-maximizing strategies for coalition and peer formation, the solution is always sub-optimal. Peer formation, based on only application layer information, also results in inefficient use of network bandwidth. When multiple recipient-peers try to access the same file from same source-peer simultaneously, contention may occur on the shared wireless channel. On the discovery of multiple source-peers, corresponding recipient-peer may choose optimal source-peer in favor of increased network throughput. We formulate the joint peer selection and utility maximization problem as a mixed integer nonlinear programming (MINLP) framework. We also propose penalty-based heuristic genetic algorithm (GA) to solve the MINLP. The results show that our favorable-peer selection strategy results in higher aggregate throughput by selecting optimum source-peers with better load distribution and minimum interference.
Unilateral Cleft Lip and Nose Repair; Closed Approach Dallas Protocol Completed Patients

Unilateral primary lip/nose repair, closed approach using the Dallas protocol presents step-by-step technique with 10 completed cases. Adjustments and corrections frequently used in the lip/nose repair are presented. A discussion of various techniques used today with their advantages and disadvantages is reviewed. Ten completed cases are presented with their long-term outcomes. Recommendations for developing countries regarding this and other techniques are made for the care of the unilateral primary cleft lip, nose, alveolus, and palate.
An Instrument for the Measurement Of Infant‐Adult Synchrony

The Dyadic Mini Code (DMC) is designed to measure levels of synchrony in early infant-adult interaction. Based on important components of synchronous interaction reported in the literature, the instrument consists of six items, a total score, and a summary rating. Videotapes of 38 term and preterm mother-infant pairs were studied to establish reliability and validity of the tool. Interrater reliability was determined using Cohen's kappa and percent of agreement; kappa was . 86 for the total score and ranged from . 63 to . 92 for each of the six items. A discrimination index, applied to each item, indicated that four items are good predictors of the final rating and two require further testing. Dyadic Mini Code summary ratings compared to mean coherence values computed from Monadic Phase Scale (Tronick, Als, & Brazelton, 1980) scores on the same data yielded moderate concurrent validity; point bi-serial analysis, rpb = . 488, p < . 01; and X2 = 4.878, df = 1, Fisher's exact test (1-tail) = p < . 05. Construct validity was demonstrated by finding that the instrument discriminated between preterm and term dyads according to expected differences, X2 = 4.071, df = 1, Fisher's exact test (1-tail) = p < . 05. Further refinement of the DMC is indicated.
Effect of Syndesmosis Injury in SER IV (Weber B)–Type Ankle Fractures on Function and Incidence of Osteoarthritis

Background: Syndesmosis fixation of Lauge-Hansen SER IV, Weber B ankle fractures is controversial. This study compared a matched pair of SER IV patients with stress proven syndesmotic injuries with the same number without stress proven syndesmotic injury in terms of functional, pain, and radiologic result. Methods: The study was based on a RCT-study comparing syndesmotic transfixation to no fixation in SER IV (Weber B)–type ankle fractures. Twenty-four patients with a syndesmosis injury found on the intraoperative 7.5 Nm standardized external rotation test were compared to 24 patients with a stable syndesmosis. The pairs were matched by fracture morphology, sex, and age. The primary outcome measure was ankle function as assessed by the Olerud–Molander score. Weight-bearing plain radiographs and 3T MRI of the injured ankle were used to assess ankle joint congruity and osteoarthritis, according to Kellgren-Lawrence classification and cartilage defects. Results: The Olerud–Molander score was 86 in syndesmosis injury patients and 90 in patients with normal syndesmosis (P = .28). The incidence of ankle joint osteoarthritis on plain radiographs was not significantly different between the groups (Grade I 5% vs 21%; Grade II 86% vs 75%; Grade III 9% vs 4%; P = .34). MRI scans showed TC joint cartilage defects in 54% of the patients: 12 (56%) in the syndesmosis injury group vs 13 (54%) in the group without syndesmosis injury (P > .9). Conclusion: After 4 to 6 years of follow-up, clinical and radiologic outcome were not different in patients with syndesmosis injury compared to patients with stable syndesmosis in SER IV (Weber B)–type ankle fractures. Level of Evidence: Prognostic Level II, comparative study.
Therapeutic drug monitoring of modified release once daily tacrolimus in de novo renal transplant with conversion to a twice daily generic in the stable period

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Implication of HR components on Employee Performance: A comparative analysis of Public & Private Sector employees

: Globalization and technological advancement has made market competitive. To survive in market every organization needs to maintain high performance. It can only be possible when the employees working in organization perform more efficiently than competitors. Increase in competition of market has made employee performance a big question to organization for its survival in global competition. Study reveals performance of employee is affected by various component of human resource management i.e. Job satisfaction, leadership, training &development and work stress. HRM is managing people effectively and efficiently to achieve strategic aims of organization. This study focuses on analyzing the impact of HR components on performance of employee. It also compares impact of HR component respect to public and private organizations. The study finds HR components have significant positive relationship with employee performance except work stress. Study also interprets findings irrespective of organization type. Implication of HR components on employee performance: A comparative analysis of public and sector employees. Pakistan Administrative Review,
Inappropriate reference intervals for carboxyhemoglobin at some Florida hospitals.

We recently conducted an investigation of unintentional carbon monoxide (CO) poisonings in hurricane-affected areas of Florida during the 2004 hurricane season (1). During this process, we reviewed the medical records of 167 persons treated for CO poisoning at 10 hospitals around the state, including 2 with hyperbaric oxygen chambers. Blood carboxyhemoglobin (HbCO) concentrations are used to confirm the diagnosis of CO poisoning, although these concentrations are not always well correlated with clinical manifestation or outcome. However, a 1995 survey of 23 hospitals and laboratories in the Boston area …
Developing social virtual worlds using NetEffect

This paper describes NetEffect, an infrastructure for developing, supporting, and managing large, media-rich, 3-D social virtual worlds for use by several thousand geographically-dispersed users using low-end computers (PCs) and modems. It has been modularized into six components, in the form of a toolkit, which simplifies the development of network-based virtual worlds. NetEffect partitions a whole virtual world into communities, allocates these communities among a set of servers, and migrates clients from one server to another as clients move through the communities. It devotes special attention to minimizing the network traffic, particularly the traffic that must go through servers. HistoryCity, a virtual world for children, has been developed on NetEffect, which is being beta-tested for deployment in Singapore.
APC-dependent suppression of colon carcinogenesis by PPARγ

Activation of PPARγ by synthetic ligands, such as thiazolidinediones, stimulates adipogenesis and improves insulin sensitivity. Although thiazolidinediones represent a major therapy for type 2 diabetes, conflicting studies showing that these agents can increase or decrease colonic tumors in mice have raised concerns about the role of PPARγ in colon cancer. To analyze critically the role of this receptor, we have used mice heterozygous for Pparγ with both chemical and genetic models of this malignancy. Heterozygous loss of PPARγ causes an increase in β-catenin levels and a greater incidence of colon cancer when animals are treated with azoxymethane. However, mice with preexisting damage to Apc, a regulator of β-catenin, develop tumors in a manner insensitive to the status of PPARγ. These data show that PPARγ can suppress β-catenin levels and colon carcinogenesis but only before damage to the APC/β-catenin pathway. This finding suggests a potentially important use for PPARγ ligands as chemopreventative agents in colon cancer.
Treating Tobacco Use and Dependence

This Quick Reference Guide for Clinicians contains strategies and recommendations from the Public Health Service-sponsored Clinical Practice Guideline, Treating Tobacco Use and Dependence. The guideline was designed to assist clinicians; smoking cessation specialists; and health care administrators, insurers, and purchasers in identifying and assessing tobacco users and in delivering effective tobacco dependence interventions. It was based on an exhaustive systematic review and analysis of the extant scientific literature from 1975-1999, and uses the results of more than 50 meta-analyses. This Quick Reference Guide summarizes the guideline strategies for providing appropriate treatments for every patient. Effective treatments for tobacco dependence now exist, and every patient should receive at least minimal treatment every time he or she visits a clinician. The first step in this process—identification and assessment of tobacco use status—separates patients into three treatment categories: (1) patients who use tobacco and are willing to quit should be treated using the “5 A’s” (Ask, Advise, Assess, Assist, and Arrange); (2) patients who use tobacco but are unwilling to quit at this time should be treated with the “5 R’s” motivational intervention (Relevance, Risks, Rewards, Roadblocks, and Repetition); and (3) patients who have recently quit using tobacco should be provided relapse prevention treatment.
Phenotypic specificity of aneuploid states in Aspergillus nidulans.

Hyperhaploid and hyperdiploid colonies of Aspergillus nidulans , disomic or trisomic for one or more chromosomes respectively, are abnormal in phenotype and somatically unstable. It has been shown for Glasgow strains that a different specific phenotype arises, depending upon which chromosome is additional (Pollard, Kafer & Johnston, 1968). This report extends this system and shows that irrespective of the residual genetic background of the isolate, the system of phenotypic specificity applies.
Robust sliding mode control for fuzzy conjugate Lorenz chaotic system

The conjugate Lorenz chaotic system is a newly coined chaotic system. In this paper, the Takagi-Sugeno (T-S) fuzzy model for the chaotic system is presented; then based on Lyapunov stability theory and using the linear matrix inequalities (LMIs) technique, the robust sliding mode controller for the T-S fuzzy conjugate Lorenz system with uncertainties was designed. The obtained sliding mode controller guarantees the global fuzzy conjugate Lorenz system confined on the sliding surface is asymptotically stable. Finally, numerical simulations are given to illuminate the effectiveness of the theoretical results.
Impact of HR Practices on Customer Satisfaction

This research examined organizational commitment as a mediator between HR practices and customer satisfaction of 3 job groups from 13 banking companies. This research by the authors was undertaken to explore the unknown and to introduce an important mediating factor ‘Group Commitment’. Interest in strategic human resource management (SHRM) has heightened as a result of a number of studies that found a relationship between HR systems variously labeled “high performance”, “commitment”, or “involvement” models and firm financial performance. Both commitment level and consensus were predicted to influence customer satisfaction. Results found that commitment level mediated the relationship between HR practices and customer satisfaction. There is from the research a positive relationship between • HR practices and organizational commitment • Commitment and customer satisfaction ratings • HR practices and customer satisfaction. Intriguingly the influence of HR practices on customer satisfaction is mediated by organizational commitment. Therefore HR practices can positively enhance customer satisfaction by improving commitment and involvement in the employees. Although consensus on HR practices should theoretically affect the consensus of commitment that develops, the researchers did not find support for this hypothesis. Similarly, no effect for consensus of commitment was found on consensus of customer satisfaction, a measure of service consistency. Collectively, these results suggest that it is the overall mean level that is most important when considering commitment. There are several possible empirical explanations that could account for why the researchers did not find an effect for consensus. The small sample size could mean that the researchers do not have enough power to detect an effect of consensus. Another possible explanation could be the restricted range of commitment and customer satisfaction measures. Future research should seek additional performance outcomes at the job level from larger samples and increase statistical power. Use of a one item rating of customer satisfaction is also problematic. Future research should seek to develop fuller measures of customer satisfaction. Another limitation of the current study involves the use of agreement on “existence” of practices as a proxy for “strength” of practices or index of the communication quality.
Evaluating change orders and their impacts on construction project performance in Lagos, Nigeria

The construction of a project is a complex process, which could make orders unavoidable. Identifying the causes of change orders on construction projects is therefore imperative in order to avoid potential changes in future. This study investigated change orders and construction project performance in Lagos state, focusing on the causes of change orders on construction projects. This is to assess their impact on construction project and minimize their occurrences. The objectives are to investigate the causes of change orders; to examine the important measures to control change orders; to examine the impact of change orders on construction projects and to examine the level of occurrence. The research was carried out using convenience sampling technique. Questionnaire survey was used to elicit information in which 50 questionnaires were distributed to the building construction professionals in Lagos state. A total of 45 questionnaires representing a 90% response rate were analysed using frequency, mean and standard deviation. The result indicated that the major cause of change orders is change of plan or scope of work, the most important measure to control change orders is involving all the parties involved in projects, and the major adverse impact of change orders on construction projects is cost overrun. Conclusively, various causes of change orders can be curtailed through awareness and adequate participation of project parties. It is recommended that practitioners should be cautious of the causes of change orders on construction projects. Construction professionals should as well adopt proper planning among parties involved to control change orders. Keywords: Change Orders, Measures, Impact, Causes, Occurrence