Miracle-dz/UniFAR-FTUs huggingface dataset:
Propagation of the yeast prion‐like [psi+] determinant is mediated by oligomerization of the SUP35‐encoded polypeptide chain release factor.

The Sup35p protein of yeast Saccharomyces cerevisiae is a homologue of the polypeptide chain release factor 3 (eRF3) of higher eukaryotes. It has been suggested that this protein may adopt a specific self‐propagating conformation, similar to mammalian prions, giving rise to the [psi+] nonsense suppressor determinant, inherited in a non‐Mendelian fashion. Here we present data confirming the prion‐like nature of [psi+]. We show that Sup35p molecules interact with each other through their N‐terminal domains in [psi+], but not [psi‐] cells. This interaction is critical for [psi+] propagation, since its disruption leads to a loss of [psi+]. Similarly to mammalian prions, in [psi+] cells Sup35p forms high molecular weight aggregates, accumulating most of this protein. The aggregation inhibits Sup35p activity leading to a [psi+] nonsense‐suppressor phenotype. N‐terminally altered Sup35p molecules are unable to interact with the [psi+] Sup35p isoform, remain soluble and improve the translation termination in [psi+] strains, thus causing an antisuppressor phenotype. The overexpression of Hsp104p chaperone protein partially solubilizes Sup35P aggregates in the [psi+] strain, also causing an antisuppressor phenotype. We propose that Hsp104p plays a role in establishing stable [psi+] inheritance by splitting up Sup35p aggregates and thus ensuring equidistribution of the prion‐like Sup35p isoform to daughter cells at cell divisions.
Lessons Learned: Transfer of the High‐Definition Circulating Tumor Cell Assay Platform to Development as a Commercialized Clinical Assay Platform

Planning and transfer of a new technology platform developed in an academic setting to a start‐up company for medical diagnostic product development may appear daunting and costly in terms of complexity, time, and resources. In this review we outline the key steps taken and lessons learned when a technology platform developed in an academic setting was transferred to a start‐up company for medical diagnostic product development in the interest of elucidating development toolkits for academic groups and small start‐up companies starting on the path to commercialization and regulatory approval.
Integrated Time-Resolved Fluorescence and Diffuse Reflectance Spectroscopy Instrument for Intraoperative Detection of Brain Tumor Margin

Time-resolved fluorescence (TRF) and diffuse reflectance (DR) spectroscopy are two optical biopsy modalities that have been studied in tumor diagnosis. Combination of TRF and DR spectroscopy allows us to obtain more features such as fluorescence intensity, lifetime, and optical properties; thus, potentially improving the tissue diagnostic accuracy. In this paper, an integrated TRF-DR spectroscopy instrument was developed to acquire TRF spectra as well as spatially resolved diffuse reflectance spectra in sequence for intraoperative detection of brain tumor margin. The performance of TRF-DR spectroscopy instrumentation was calibrated and evaluated using endogenous biomolecules, tissue phantoms, and ex vivo brain tumor specimens. The results demonstrated that the TRF-DR system is capable to retrieve the fluorescence and optical properties accurately.
Gait Parameters Associated With Responsiveness to Treadmill Training With Body-Weight Support After Stroke: An Exploratory Study

Background Task-specific training programs after stroke improve walking function, but it is not clear which biomechanical parameters of gait are most associated with improved walking speed. Objective The purpose of this study was to identify gait parameters associated with improved walking speed after a locomotor training program that included body-weight–supported treadmill training (BWSTT). Design A prospective, between-subjects design was used. Methods Fifteen people, ranging from approximately 9 months to 5 years after stroke, completed 1 of 3 different 6-week training regimens. These regimens consisted of 12 sessions of BWSTT alternated with 12 sessions of: lower-extremity resistive cycling; lower-extremity progressive, resistive strengthening; or a sham condition of arm ergometry. Gait analysis was conducted before and after the 6-week intervention program. Kinematics, kinetics, and electromyographic (EMG) activity were recorded from the hemiparetic lower extremity while participants walked at a self-selected pace. Changes in gait parameters were compared in participants who showed an increase in self-selected walking speed of greater than 0.08 m/s (high-response group) and in those with less improvement (low-response group). Results Compared with participants in the low-response group, those in the high-response group displayed greater increases in terminal stance hip extension angle and hip flexion power (product of net joint moment and angular velocity) after the intervention. The intensity of soleus muscle EMG activity during walking also was significantly higher in participants in the high-response group after the intervention. Limitations Only sagittal-plane parameters were assessed, and the sample size was small. Conclusions Task-specific locomotor training alternated with strength training resulted in kinematic, kinetic, and muscle activation adaptations that were strongly associated with improved walking speed. Changes in both hip and ankle biomechanics during late stance were associated with greater increases in gait speed.
Genetic control of Endosperm Balance Number (EBN) in the Solanaceae based on trisomic and mutation analysis.

The genetic control of Endosperm Balance Number (EBN) was studied using trisomics and induced mutation. In order to induce and detect a change in a factor determining the EBN of the male, pollen from tetraploid Datura was irradiated and used to pollinate diploids. No triploids were produced in over 70 000 fertilizations. At least 70 would have been expected if the deletion of the function of a single gene could change the EBN. An attempt was made to find a particular chromosome that could alter the EBN of the female when it was present as the extra chromosome in 2x + 1 x 4x crosses. In tests of trisomics in both Datura and Solanum (potato) no chromosome could be found that changed the EBN. Therefore, it is concluded that more than one gene and more than one chromosome is involved in determining EBN. Key words : crossing barriers, endosperm, Datura, potato, speciation.
IDENTIFICATION OF MASTIGOCLADUS LAMINOSUS GENES ASSOCIATED WITH ENHANCED NITROGEN FIXATION PERFORMANCE

Hutchins, Patrick, M.S., June 2014 Cellular, Molecular, and Microbial Biology IDENTIFICATION OF MASTIGOCLADUS LAMINOSUS GENES ASSOCIATED WITH ENHANCED NITROGEN FIXATION PERFORMANCE Chairperson: Dr. Scott Miller Understanding population variation for fitness-related traits is important for our comprehension of evolutionary adaptation and of how populations respond to environmental change. Here, I investigate variation in nitrogen fixation performance for an ecologically-variable population of the cyanobacterium Mastigocladus laminosus from White Creek, a nitrogen-limited, geothermally-influenced stream in Yellowstone NP. I next take a population genomics approach to identify candidate loci associated with superior performance. Variation among strains and temperature dependence of the nitrogen fixation process were the most important factors in a linear mixed effects model. Absolute and relative measures of genetic differentiation between strains from the upper quartile of nitrogen fixation performance and the other 75% of strains showed that only a small subset of loci were associated with superior nitrogen fixation. Most notably, the strains that fixed the most nitrogen contained a premature stop codon in a regulatory histidine kinase gene, but this allele was present at low frequency in other strains. Because this nonsense mutation eliminates many important functional sites in the protein, this allele is expected to be non-functional. Both the full-length and the putative null allele, as well as a third recombinant allele, were expressed during nitrogen step-down and in the presence of combined nitrogen. Future studies will investigate whether the nonsense mutation results in transcriptional rewiring that is favorable for nitrogen fixation.
Alpha‐subunit and human chorionic gonadotropin‐β immunoreactivity in patients with malignant endocrine gastroenteropancreatic tumours

Abstract. In the serum of patients with malignant endocrine gastroenteropancreatic (GEP) tumours, both α‐subunit (α‐SU), common to all glycoprotein hormones, as well as free β‐subunit of human chorionic gonadotropin (hCG‐β) have been reported to be elevated in a substantial fraction. Both have been discussed as markers of malignancy in these neoplasms. In the present study we evaluated the diagnostic significance of α‐SU and hCG‐β as serum markers in patients with malignant endocrine gastroenteropancreatic tumours.
Speed and Capacity of Language Processing Test: Normative Data From an Older American Community-Dwelling Sample

This study presents normative data for the Speed and Capacity of Language Processing (SCOLP) test from an older American sample. The SCOLP comprises 2 subtests: Spot-the-Word, a lexical decision task, providing an estimate of premorbid intelligence, and Speed of Comprehension, providing a measure of information processing speed. Slowed performance may result from normal aging, brain damage (e.g., head injury), or dementing disorders or may represent the intact performance of someone who always performed at the low end of normal. The SCOLP enables the clinician to differentiate between these possibilities. Adequate age-appropriate norms to differentiate dementia from normal aging do not exist. We present data from 424 older community-dwelling Americans (75-94 years old). The results confirm that information processing speed slows with increasing age. By contrast, increasing age has little effect on lexical decision. Thus, our data suggest that the SCOLP shows promise as a tool to help distinguish between normal aging and the early stages of dementia.
A new hydrated ammonium hydroxyborate, (NH(4))(2)[B(10)O(14)(OH)(4)].H(2)O.

The structure of a new synthetic compound, diammonium tetrahydroxydecaborate monohydrate, has been determined by single-crystal X-ray diffraction. It crystallizes in triclinic space group P-1 and all atoms occupy general sites. The title compound is composed of [B(10)O(15)(OH)(4)](4-) ions as the fundamental building blocks, and these are linked end-to-end by sharing two common O atoms, thus producing infinite chains of composition [B(10)O(14)(OH)(4)](n)(2n-). These chains are linked by hydrogen bonds, thus forming borate sheets. Water molecules and ammonium ions between these sheets connect adjacent sheets via hydrogen bonds.
Immunoelectron microscopy in mycosis fungoides and benign dermatoses. Expression of CD3, CD4 and CD7 receptors.

UNLABELLED Mycosis fungoides is an epidermotropic cutaneous T-cell lymphoma (CTCL). Specimens for presented study were taken from sixteen patients with MF confirmed by immunohistochemical methods and PCR and from nine patients with benign dermatoses. To demonstrate CD3, CD4 and CD7 antigens immunogold method was used. We saw morphological differences between lymphocytes from MF and benign dermatoses. In MF, CD3 and CD4 were present rather in form of clusters placed on the surface of cell. On the contrary -CD3 to CD7 distribution analysis showed that these antigens were present rather individually, however there were seen clusters as well. In MF tumor stage labelling decreased in following order: CD7, CD3 and CD4. We also found internalisation of studied antigens via the coated structures of the cell membrane -especially in tumor stage. In benign dermatoses the majority of all receptors was present on the cell membrane. Our work showed differences in localization of studied antigens, between MF stages, what can suggest their possible translocation in cells. We also found loss of CD3 and CD7 antigens in tumor stage what might be use as adiagnosis tool for this disease. KEYWORDS mycosis fungoides, benign dermatoses, CD3, CD4 and CD7 receptors, immumogold labelling.
Order Reduction by Explicit Moment Matching Based on State Variable Approach

The paper presents a sistematic procedure for order reduction needed in an efficient analog large-scale circuit simulation. The procedure uses a reduced number of the original circuit moments to obtain an accurate approximation of the circuit function corresponding to an equivalent reduced-size circuit. An efficient program for state equation formulation and transfer function generation allow the computation of the original circuit moments. Some examples are done to illustrate the method.
Care Management

this chapter includes nine activities and three assessments for training community health workers (CHWs) to provide care management services Care management (sometimes referred to as care coordination or case management) involves supporting clients to establish clear goals and to identify their needs for specific resources or services Clients are supported to take action to achieve their goals and access desired resources
Transfer RNA recognition by aminoacyl‐tRNA synthetases

The aminoacyl‐tRNA synthetases are an ancient group of enzymes that catalyze the covalent attachment of an amino acid to its cognate transfer RNA. The question of specificity, that is, how each synthetase selects the correct individual or isoacceptor set of tRNAs for each amino acid, has been referred to as the second genetic code. A wealth of structural, biochemical, and genetic data on this subject has accumulated over the past 40 years. Although there are now crystal structures of sixteen of the twenty synthetases from various species, there are only a few high resolution structures of synthetases complexed with cognate tRNAs. Here we review briefly the structural information available for synthetases, and focus on the structural features of tRNA that may be used for recognition. Finally, we explore in detail the insights into specific recognition gained from classical and atomic group mutagenesis experiments performed with tRNAs, tRNA fragments, and small RNAs mimicking portions of tRNAs. © 2000 John Wiley & Sons, Inc. Biopoly 52: 1–28, 1999
Performance and Mechanisms of Excess Sludge Reduction in the Cannibal™ Process

In order to study the performance and mechanisms of excess sludge reduction in the activated sludge that incorporates the Cannibal Process, laboratory activated sludge systems incorporating an anaerobic bioreactor into the sludge recycle stream were operated. In this study, the solids production in the Cannibal system was about 35-40% of the conventional system under steady state conditions. The reduction in waste sludge was optimized when the interchange rate, (the ratio of sludge fed from the activated sludge system to the bioreactor compared to the total mass in the activated sludge system) was set at about 10 %. It was found that the release of protein from the anaerobic bioreactor was greater than that from the aerobic bioreactor. The SOUR data suggested that the released protein from the anaerobic bioreactor was easily degraded when the sludge was returned to the activated sludge system. It was also found that when the proportion of sludge added to the anaerobic bioreactor in batch tests was approximately 10%, the protein release was about 30 mg/L. When the proportion of sludge added was increased to 26 to 41%, the release was reduced to 10 and 6 mg/L, respectively. Within 30 hours, the protein release was complete. This suggests that there is an optimum or maximum amount of recycle or interchange (~10%) for the process to function best.
Data Security for Unreliable Clouds Using Reliable Encryption

A data distributor has given sensitive data to a set of supposedly trusted agents (third parties). Some of the data are leaked and found in an unauthorized place (e.g., on the web or somebody’s laptop). We propose data allocation strategies that improve the probability of identifying leakages. In Image and Video we can apply watermarking for detecting guilty agent. All the data will be stored on the cloud so Reliable Re-encryption in unreliable clouds technique is used. In this data owner store encrypted data in the cloud, and issue decryption keys to authorized users. When a user is revoked, the data owner will issue re-encryption commands to the cloud to re-encrypt the data, to prevent the revoked user from decrypting the data, and to generate new decryption keys to valid users, so that they can continue to access the data. In this project a time based re-encryption scheme is used for cloud servers to automatically re-encrypt data based on their internal clocks.
Glowing Connection Experiments With Alternating Currents Below 1${\rm A}_{\rm rms}$

It was experimentally shown that a glowing connection can develop in an electrical switch at a terminal connection comprised of a connector that utilizes a spring action against a conductor to provide electrical contact in household electrical appliances where vibration exists, and when the connection is loose. This can happen at a current flow such as 0.6 Arms at 120-V. Further, the glow can provide sufficient heat to degrade plastic in contact with the terminal components. Violent arcing involving intense gasification can then develop on nearby plastic areas, sometimes accompanied by intensely glowing areas inside the plastic. Sometimes the intense glowing areas inside plastic develop without the violent arcing. The intense arcing or the glow inside plastic often develops into flaming, which can be of sufficient intensity and duration to ignite combustible materials near the connection.
Beschäftigungsmultiplikatoren und die Besetzung von Arbeitsplätzen in Österreich

Mit dem vom WIFO entwickelten DYNK-Modell, einem disaggregierten, makrookonomischen Modell der osterreichischen Wirtschaft, werden Wertschopfungs- und Beschaftigungsmultiplikatoren fur unterschiedliche Endnachfragekategorien berechnet. Die Beschaftigungseffekte werden uber den Beschaftigungsmultiplikator getrennt fur Selbstandige und Unselbstandige dargestellt; fur die Gruppe der Unselbstandigen erfolgt eine weitere Differenzierung nach Alter und Geschlecht sowie gesicherten bzw. neu geschaffenen Stellen. Fur die neu geschaffenen Arbeitsplatze werden mit dem Vacancy-Chain-Ansatz unter Verwendung des Markov-Modells die Zahl der notwendigen Zugange an Personen und deren Verteilung auf einzelne arbeitsmarktrelevante Personengruppen bestimmt.
Modèles $p$-minimaux des variétés abéliennes

© Association des collaborateurs de Nicolas Bourbaki, 1962, tous droits réservés. L’accès aux archives du séminaire Bourbaki (http://www.bourbaki. ens.fr/) implique l’accord avec les conditions générales d’utilisation (http://www.numdam.org/conditions). Toute utilisation commerciale ou impression systématique est constitutive d’une infraction pénale. Toute copie ou impression de ce fichier doit contenir la présente mention de copyright.
Diabetes insipidus secondary to intracranial sarcoidosis confirmed by low‐field magnetic resonance

A 32‐year‐old woman who had been diagnosed as having pulmonary sarcoidosis 18 months previously presented with thirst and polyuria. The diagnosis of diabetes insipidus was confirmed biochemically and magnetic resonance imaging (MRI) revealed sarcoid involvement of the pituitary stalk. © Academic Press, Inc.
The NAD(P)H: quinine oxidoreductase 1 (NQO1) gene 609 C>T polymorphism is associated with gastric cancer risk: evidence from a case-control study and a meta-analysis.

UNLABELLED The association between the NAD(P)H quinone oxidoreductase 1 (NQO1) gene C609T polymorphism (rs1800566) and gastric cancer has been widely evaluated, but a definitive answer is so far lacking. We first conducted a case-control study to assess this association in a large Han Chinese population, and then performed a meta-analysis to further address this issue. Although our case-control association study indicated no significant difference in the genotype and allele distributions of C609T polymorphism between gastric cancer patients and controls, in the meta analysis involving 4,000 subjects, comparison of alleles 609T and 609C indicated a significantly increased risk (46%) for gastric cancer (95% confidence interval (95%CI) for odds ratio (OR)=1.20- 1.79) in individuals with the T allele. The tendency was similar to the homozygote (OR=1.81, 95%CI: 1.16-2.84), dominant models (OR=1.41, 95%CI: 1.12-1.79), as well as recessive model (OR=1.58, 95%CI: 1.06-2.35). Stratified analysis by study design demonstrated stronger associations in population-based than in hospital-based studies. And ethnicity-based analysis demonstrated a significant association in Asians. We conclude that the NQO1 gene C609T polymorphism increases the risk for gastric cancer, especially in Asian populations.
Annual changes in brain concentration of arginine vasotocin and isotocin correspond with phases of reproductive cycle in round goby, Neogobius melanostomus

Reproductive cycle of seasonally breeding fish is synchronized with changes of photoperiod and temperature in environment. We hypothesize that arginine vasotocin (AVT) and isotocin (IT) are involved in timing and synchronization of seasonal reproductive activity in the round goby (Neogobius melanostomus). To verify this hypothesis, we examined the annual profiles of brain AVT and IT in round goby males and females in relation to their reproductive cycle. Wild round gobies were exposed to annual environmental changes in their natural habitats from where they were sampled monthly over a year. AVT and IT were measured using HPLC with fluorescence detection preceded by solid-phase extraction. This study shows seasonal variations in brain AVT and IT levels. Profiles of changes were similar in males and females: the peak of AVT was observed before spawning in March-April, whereas that of IT during spawning in May–June. Furthermore, the lowest AVT level was noted out of breeding season from November to January, while the level of IT decreased immediately at the end of the spawning. The results show that high AVT levels correlate with pre-spawning period whereas the highest IT levels correspond to spawning. A significant decline in AVT and IT in non-spawning season coincided with the quiescent phase of gametogenesis in both sexes.
LAMP in L.A.'s skid row: a model for community-based support services.

In most states, we reneged on our promise to provide community-based program support to persons deinstitutionalized from psychiatric hospitals. Now many people are blaming those with mental illness for their failure to thrive and have begun creating new laws and methods for reinstitutionalization. This chapter describes a community-based program that is demonstrably effective. It involves the full participation of homeless people diagnosed with mental illness and attests to what can work.