Miracle-dz/UniFAR-FTUs huggingface dataset:
“New” inhalant plant allergens*

Specific IgE measurements obtained from patients suffering from respiratory allergy (n = 952) show that, despite similar climatic conditions, there are clear regional differences in pollen sensitization between North Rhine-Westphalia and Bavaria. The data on sensitization levels and pollen concentration was taken from the research and development project Ufoplan 3710 61 228 of the German Environment Agency for North Rhine-Westphalia and Bavaria (2011 – 2014). Most poly-sensitized patients have already shown sensitization, both in the form of cross-reactivity and species-specific sensitization, to “new” pollen allergens, such as Bermuda grass and olive tree. These plants are currently not common in Germany, but may become considerably more widespread due to the increase in average yearly temperatures caused by the global warming. The other “new” aeroallergens discussed here are plants that can be found throughout Germany, such as nettle, cypress, and pine. Their current sensitization levels are higher than 8%; however, their clinical impact appears to be underestimated. For clinical practice it is important to identify when patients’ symptoms are typically severe and which regional plants might be responsible for the patients’ complaints in this period of time, as this affects further diagnostic strategy. Allergens having an immune effect can then be targeted by specific immunotherapies. The information on complaints of the patients should be regularly recorded in symptom diaries. Recording this information for at least 1 year may allow to discover a correlation between specific types of pollen and allergy symptoms.
Changes of Main Aromatic Compounds and Fatty Acids Contents of Cucumber Fruits during Development in Solar-greenhouse

The contents of the primary aromatic compounds,linolenic acid and linoleic acid in cucumber(Cucumis sativus L.cv 'Xintaimici')fruits developed at different monthes,and the changes of them during development in January were investigated.The result showed that cucumber fruits contain 2E,6Z-nonadienal,2E-nonenal,2E-hexenal,2,6-nonadienol,hexanal,pentanal,2-pentanenol,3,6-nonadienol with higher relative contents of volatile compounds.The relative content of 2E,6Z-nonadienal,the characteristic flavor compounds of cucumber fruits developed in May was the highest,and in November the lowest.The relative contents of 2,6-nonadienol and 3,6-nonadienol,with cucumber aroma,and the ratio of 2E,6Z-nonadienal/2E-nonenal,which have close correlation with flavor quality of cucumber fruits developed in January were the highest,and November the lowest.The linolenic acid and linoleic acid content which developed in January were more than those of in May,indicated that it was advantage to accumulation of unsaturated fatty acids under low temperature and light intensity condition.The relative content of 2E,6Z-nonadienal,2,6-nonadienol and 3,6-nonadienol,and the ratio of 2E,6Z-nonadienal/2E-nonenal all increased greatly during fruits development,and came to the peak at the market maturity.However,they were decreased as reaping period delayed.The contents of 2E-nonenal,2E-hexenal,hexanal,pentanal,et al.decreased as fruits development,and those of 2E-nonenal,2E-hexenal and hexanal were to the lowest,then increased fleetly.The inolenic acid and linoleic acid contents and their ratio in cucumber fruits during development changed as a similar rule as 2E,6Z-nonadienal and 2,6-Nonadienol content and their ratio.The flavor quality of cucumber fruits had distinct relativity with linolenic acid and linoleic acid contents.
Analysing gene expression : a handbook of methods : possibilities and pitfalls

VOLUME 1.Foreword. Preface.Table of Contents.List of Contributors.1. Basic concepts of gene expression.2. Sample preparation and supplementary tools.3. Methods for analysing mRNA expression.VOLUME 2.4. High-throughput and industrial methods for mRNA expression analysis.5. Protein expression analysis.6. Methods for mRNA and protein expression analysis in situ and in vivo.7. Computational methods and bioinformatic tools.Index.
Assessment of the quality of life of patients with multiple sclerosis

Introduction. Multiple sclerosis (lat. sclerosis multiplex – MS) is one of the most common causes of non-traumatic disability in the group of “young adults”. The incidence is estimated at 33 people per 100,000 inhabitants. Patients have to deal with its consequences for the rest of their lives, which affects many aspects of their lives. People with MS have a low level of self-acceptance and negative self-esteem. Aim. The aim of the study was to assess the quality of life of MS patients. The following research questions were posed: Is there a correlation between the assessed quality of life of patients with multiple sclerosis and the degree of disability? What factors determine the quality of life of an MS patient? Material and methods. The study involved 128 people suffering from MS, including 87 women and 41 men, aged 20-67. The average age of respondents was 38.2 (± 11). The tests were carried out using a diagnostic survey. The obtained test results were subjected to statistical analysis using the STATISTICA PL V.12. Results. People with multiple sclerosis determine their level of quality of life as medium, the biggest impact on this assessment are disease ailments, emotional state and fatigue. The degree of disability does not affect the assessment of the quality of life of a patient with multiple sclerosis. Conclusions. The degree of disability is not a factor that influences the quality of life assessment of a patient with multiple sclerosis The sex of the patient and the type of disease are factors that influence the quality of life assessment of a patient with multiple sclerosis. Keywords
Depósito electrolítico de metales: influencia de aditivos y complejantes en el crecimiento de los cristales y en la calidad de los depósitos

Fil: Pary, Paola. Provincia de Buenos Aires. Gobernacion. Comision de Investigaciones Cientificas. Centro de Investigaciones en Tecnologia de Pinturas. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - La Plata. Centro de Investigaciones en Tecnologia de Pinturas; Argentina
The effects of hypoxia on cyclooxygenase-2 expression and eicosanoid synthesis / by Maryanne Demasi.

S ARISII{G FROM THIS THESIS EICOSANOID PRODUCTION IN HUMAN MONOCYTES: INVOLVEMENT OF COX-I AND COX-2. Demasi-M, Cleland-LG, Caughey-GE, James-MJ Proceedings of Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists, Newcastle, NSW (2000). ASSAY OF COX-I AND COX-2 ACTIVITY IN HUMAN MONOCYTES. Demasi-M, Cleland-LG, Caughey-GE, James-MJ Australian Rheumatology Association National Conference (2001). EFFECT OF HYPOXIA ON MONOCYTE COX-2 EXPRESSION AND EICOSANOID PRODUCTION. Demasi-M, Cleland-LG, Caughey-GB, James-MJ Australian Rheumatology Association State Conference (2001). HYPOXIA IN INFLAMED JOINTS; MECHANISTIC IMPLICATIONS. DCMASi.M, Cleland-LG, Cook-Johnson-Rl, Caughey-GE, James-MJ. Medical Staff Society Research Presentation Royal Adelaide Hospital (2002). (Awarded the Medical Staff Society Res earch prize for best s cientifi c pres ent ation). MECHANISM FOR INCREASED COX-2 EXPRESSION IN HYPOXIC MONOCYTES. Demasi-M, Cleland-LG, Cook-Johnson-Rl, Caughey-GE, James-MJ. Australian Rheumatology Association State Conference (2002).
The effect of pulsatile flow on co-cultured retinal endothelial & pericyte cells

Microvascular cell fate decisions are hallmarks of the microvascular cell response to injury and play a crucial role in the pathogenesis of retinal disease. Abnormalities in retinal blood flow play a critical role in remodeling of the retinal vasculature by altering microvascular endothelial and pericyte cell fate (proliferation, apoptosis and migration). Retinal blood flow is controlled locally by vasodilators such as nitric oxide, prostacyclin and the vasoconstrictor endothelin-1 , with considerable evidence linking retinal pathologies such as Normal Tension Glaucoma and Diabetic Retinopathy to altered retinal blood flow Shear stress has previously been shown to modulate EC production of these vasoactive agents in macrovascular cells. Therefore, using a perfused transcapillary coculture of bovine microvascular retinal endothelial cells (BRECs) and bovine retinal pericytes (BRPs), we examined the acute and chronic effect of pulsatile flow on the release of these vasoactive mediators and their subsequent role in modulating retinal vascular cell fate. Acute exposure to pulsatile flow increased BREC NO, PGI2 & ET-1 formation and release Similarly, chronic exposure to pulsatile flow enhanced NO and PGI2 release while concomitantly inhibiting ET-1 in these cells In parallel studies, there was an increase in BRP apoptosis following exposure to high pulsatile flow, whereas BREC apoptosis decreased. Furthermore, the pulsatile flow-induced increases in BRP apoptosis is dependent on increased PGI2 , whereas both ET-1 and NO mediate the protective effect of increased flow on BRECs survival. Notch receptor-hgand interactions and the Hedgehog signalling pathway have been strongly implicated in vascular morphogenesis and remodelling of the embryonic vasculature, with Hedgehog acting upstream of Notch signalling during development. We therefore tested the hypothesis that Hedgehog (Hh) and Notch pathway interact to promote changes in vascular cell fate in BRECs and BRPs in vitro in response to changes in pulsatile flow.
Squelette membranaire chez Paramecium tetraurelia : caractérisation d'une nouvelle famille multigénique et analyse par les approches GFP et RNAi

Chez la paramecie, l'epiplasme se presente sous forme d'ecailles independantes disposees autour de chaque appareil ciliaire et composees d'une multitude de proteines appelees epiplasmines. Dans une premiere partie, nous avons contribue a l'annotation du genome macronucleaire de Paramecium tetraurelia a partir de la sequence de 2 epiplasmines (EPI-1 et EPI-2) et identifie 39 genes supplementaires. L'analyse des sequences indique que la signature de cette nouvelle famille est un domaine de 79 acides amines. L'expression de l'epiplasmine 1 couplee a la GFP confirme la localisation de cette proteine au niveau des ecailles epiplasmiques. La deuxieme partie porte sur l'analyse fonctionnelle d'epiplasmines a partir d'une approche ARN interference. Nous avons obtenu un phenotype caracterise par un blocage repete de la cytocinese conduisant a des organimes monstrueux qui sont finalement lyses apres 72 h d'induction. L'analyse en microscopie a fluorescence a revele l'absence de sillon de division.
THE MICROHETEROGENEITY OF PLASMA ALBUMINS. II. PREPARATION AND SOLUBILITY PROPERTIES OF SUBFRACTIONS.

In the first paper of this series (1) a model for microheterogeneity of plasma albumins was presented together with some critical evidence in support of its adoption. According to this model plasma albumin consists of a very large number of similar molecular species. The N-F transition of the protein as observed by electrophoresis, solubility, optical rotatory dispersion, and other methods is actually the resultant of a heterogeneity distribution function1 superimposed on the pH dependence of individual transitions. Each component species undergoes an immeasurably fast, highly cooperative isomerization reaction at a slightly different characteristic pH. At a specific pH within the over-all N-F transition region most of the molecules exist virtually 100% of the time in either the N or the F form. ,4 minor fraction of the molecules is in effective dynamic equilibrium between N and F forms. This last category comprises species whose individual N-F transitions occur at or close to the prevailing pH of the solution. Experiments based on the difference in solubility of N and F forms in the presence of high salt concentrations were cited (1) in substantiation of the microheterogeneity model. As a logical application of the solubility property it should be possible to fractionate the population into narrower subpopulations by removing precipitate at various values of pH within the range of the N-F transition. The subpopulations thus obtained should exhibit N-F transitions different from each other and from the parent population. Only a subpopulation precipitated symmetrically about the pH midpoint of a symmetrical distribution would be expected to display an N-F transition with a midpoint that coincides with that of the whole population. Moreover, the observed transition of any subfraction should cover a narrower pH range than that of the parent albumin. Herein we discuss the preparation of plasma albumin subfractions, their solubility properties, and related problems. A subsequent paper of this series will concern other physicochemical studies of such subfractions.2 Some electrophoretic results have already been reported (2).
Effects of peripartum propylene glycol supplementation on nitrogen metabolism, body composition and gene expression for the major proteolytic systems in skeletal muscle in transition dairy cows

Early-lactating dairy cows mobilize body protein, primarily from skeletal muscle, to provide amino acids which are directed towards gluconeogenesis and milk protein synthesis. Propylene glycol (PG) is a precursor of ruminal propionate, and our hypothesis was that its dietary inclusion could attenuate skeletal muscle wasting by reducing amino acid-driven gluconeogenesis. The major objectives of this study were to delineate the effects of preand post-partum PG supplementation in transition dairy cows on whole-body nitrogen (N) balance, urinary 3-methylhistidine (3-MH) excretion, body composition, and gene expression profiles for the major protein degradation pathways in skeletal muscle. Sixteen pregnant cows (7 primiparous and 9 multiparous) were paired based on expected calving dates and then randomly assigned within each pair to either a basal diet (control) or basal diet plus 600 mL/d of PG (PG). Diets were fed twice daily for ad libitum intake, and PG was fed in equal amounts as a top dress. All measurements were conducted at 3 time intervals starting at d -14 ± 5, d 15 and d 38 relative to calving. Propylene glycol had no effect (P > 0.05) on whole-body N balance, urinary 3-MH excretion, and body composition. However, N balance was lower (P < 0.001) at d 15 and d 38, compared to d -14. Urinary excretion of 3-MH was lower at d 14 than at d 15 (P = 0.01) and d 38 (P = 0.001). Supplemental PG had no effect (P > 0.05) on body weight (BW), and all components of empty BW. On average, cows fed both diets mobilized 19 kg of body fat and 14 kg of body protein between d -14 and d 38. Supplemental PG had no effect on mRNA abundance in skeletal muscle for mcalpain (P = 0.96) and 14-kDa ubiquitin-carrier protein E2 (14-kDa E2) (P = 0.54); however, PG supplementation down-regulated mRNA expression for μ-calpain at d 15 (P = 0.02), and tended to down-regulate mRNA expression for ubiquitin at d 15 (P =
Reconstruction of seed dispersal via modeling, seedling recruitment, and dispersal efficiency of Hemiphaga novaeseelandiae in Vitex lucens and Prumnopytis ferruginea in New Zealand

III ACKNOWLEDGEMENTS . V TABLE OF CONTENTS . VI LIST OF FIGURES . XI LIST OF PLATES . XVI LIST OF TABLES . XVII 1 LITERATURE REVIEWS, THESIS OBJECTIVES, CONTRIBUTION, AND NOMENCLATURE . 1 1.1 Review of seed dispersal and seedling distributions. 1 1.1.1 What is seed dispersal? 1 1.1.2 Why are seeds dispersed? 2 1.1.2.1 Escape hypothesis . 2 1.1.2.2 Species diversity . 3 1.1.2.3 Effects of gut passage of seeds on germination . 4 1.1.3 Ecological factors and seed dispersal . 4 1.1.3.1 Colonisation, recruitment, & ovule abortion . 4 1.1.3.1.1 Colonisation . 5 1.1.3.1.2 Recruitment . 5 1.1.3.1.3 Ovule abortion . 5 1.1.3.2 The influence of seed dispersal on population structure . 6 1.1.3.3 The influence of seed dispersal on community structure . 6 1.1.3.4 The influence of animal communities on seed dispersal . 6 1.1.3.5 The influence of habitat fragmentation on seed dispersal . 7 1.1.4 Seed and seedling distributions . 7 1.1.4.1 Seed shadows (spatial patterns of seed rain) . 7 1.1.4.2 Measuring seed shadows . 9 1.1.4.3 Measuring seedling distributions . 10 1.1.4.4 Spatial concordance of seed shadows vs. seedling recruitment . 10 1.1.4.5 Analyzing the spatial concordance of seed shadows vs. seedling recruitment . 11 1.1.4.6 Long distance seed dispersal distributions . 12 1.1.4.7 Measurement of long distance seed dispersal . 13 1.2 Review of theoretical models of seed dispersal and seedling recruitment . 14 1.2.1 Phenomenological seed dispersal models . 14 1.2.2 Mechanistic seed dispersal models . 15 1.2.3 Seedling recruitment models . 16 1.2. 4 Assessment of seed dispersal models . 16 1.3 Review of the natural history of Vitex lucens and Prumnopytis ferruginea (study trees) . 17 1.3.1 Physical descriptions . 17 1.3.2 Fruiting phenology . 18 1.3.3 Microsite preferences . 21 1.4 Review of the main dispersal vector (Hemiphaga novaeseelandiae) . 22 1.4.1 Dispersal effectiveness and efficiency . 22 1.4.2 Fruit quality preference . 24 vii 1.5 Objectives of the thesis . 24 1.6 Contribution of my research to broader theoretical questions of seed dispersal ecology . 25 1.7 Details of nomenclature . 27 2 METHODS PART 1: STUDY REGION LOCATIONS AND SITE DESCRIPTIONS . 28 2.1 Region 1: Wenderholm Regional Park (puriri only) . 28 2.1.1 Location maps. 28 2.1.2 Sites description . 30 2.2 Region 2: Waitakere Ranges (miro & puriri) . 32 2.2.1 Location maps. 32 2.2.2 Sites description . 37 2.3 Region 3: Hunua Ranges (miro only) . 41 2.3.1 Location maps. 41 2.3.2 Sites description . 43 3 METHODS PART 2: EMPIRICAL FIELD-WORK AND MATERIALS . 45 3.
Impact du vrillage sur les forces électromagnétiques dans l’entrefer : Applications aux machines asynchrones

La machine asynchrone est a ce jour la reference dans les moteurs de traction ferroviaire. De nombreuses contraintes dictees par des normes notamment au niveau du bruit acoustique poussent l’industrie a developper des moteurs plus silencieux. Le vrillage connu pour reduire les ondulations de couple semble apporter egalement une reelle solution afin de diminuer les vibrations et le bruit emis par la machine. C’est dans ce contexte que ce projet en collaboration avec Alstom-Transport situe a Ornans et le soutien de MEDEE (pole regional sur la maitrise energetique) est realise. Cette these se focalise plus particulierement sur le calcul des forces electromagnetiques dans l’entrefer a l’origine des vibrations, du bruit et du couple. Des modeles analytiques et a elements finis estimant les forces electromagnetiques et integrant le vrillage sont presentes et confrontes a des mesures experimentales de vibration, de bruit et de couple. Les modeles utilises sont valables pour les machines asynchrones a cage d’ecureuil mais sont facilement adaptables pour les machines synchrones. Ils permettent de comprendre l’origine des forces electromagnetiques responsables du bruit et de trouver des configurations de machines moins bruyantes en jouant sur divers parametres tels que : le vrillage, mais aussi le nombre de paires de poles, le nombre d’encoches au stator et au rotor, la forme des encoches, la distribution du bobinage, etc.
LASER RAMAN SPECTROSCOPY : SOME CLINICAL APPLICATIONS

Recent advances in lasers, multichannel detectors and PC-based spectroscopic instrumentation have made Raman spectroscopy an invaluable tool for characterization of clinical samples like tissue, body fluids and cytological smears. Efforts are being made at present to develop Raman spectroscopy techniques for optical pathology, early detection of neoplasia, analysis of body fluids, optical imaging, spectral markers for identifying clinical conditions, etc. Some of the results which will lead to such applications are presented in this paper.
Finding differential splice junctions in RNA-Seq data as transcriptional biomarkers for prostate cancer

Alternative RNA splicing is a naturally occurring phenomenon that has been associated with different types of cancer. Detecting splice junctions in the genome of an organism is the key to the study of alternative splicing. RNA-Seq as a high-throughput sequencing technology has recently opened new horizons on the studying of various fields of transcriptomics, such as gene expression, chimeric events and alternative splicing. In this research, we study prostate cancer from the viewpoint of splicing events as the second most common cancer in North America. We have proposed a method for differentially detecting splice junctions, and in a broader sense splice variants, from RNA-Seq data. We have designed a 2-D peak finding algorithm to combine and remove the dubious junctions across different samples of our population. A scoring mechanism is used to select junctions as features for prediction of cancer RNA-Seq data belonging to patients diagnosed with prostate cancer against benign samples. These junctions could be proposed as potential biomarkers for prostate cancer. We have employed support vector machines which proved to be highly successful in prediction of prostate cancer.
Sistemas miméticos de los cuerpos laminares epidérmicos para el tratamiento de la piel

El estrato corneo (SC) es la capa mas superficial de la epidermis. Su funcion es actuar como barrera, evitando la perdida de agua y la entrada de microorganismos. El SC esta formado por corneocitos rodeados por lipidos que se organizan en forma de laminas. Estos lipidos son ceramidas, colesterol, acidos grasos libres y sulfato de colesterol. Los cuerpos laminares epidermicos son organulos secretores responsables de la formacion de esta estructura lipidica intercelular. En muchas enfermedades de la piel como la dermatitis atopica, la funcion de estos organulos esta alterada y como consecuencia se observa una composicion lipidica irregular y una barrera cutanea ineficiente que puede ser causa y efecto de la propia enfermedad. El objetivo principal de esta tesis ha sido desarrollar un sistema lipidico que imite la morfologia, estructura y composicion de los cuerpos laminares epidermicos con el proposito de que pueda imitar tambien la funcion de estos organulos mejorando la funcion barrera. Con este objetivo se diseno una serie de sistemas lipidicos (denominados LBms) que incluian lipidos del SC ademas de un lipido con efecto antimicrobiano. Mediante criomicroscopia electronica se vio que estaban formados por vesiculas que encapsulan estructuras discoidales de manera similar a los organulos endogenos. En el presente trabajo se evaluo el efecto que tiene la aplicacion topica del LBms in vitro en piel previamente deslipidizada e in vivo tanto en voluntarios con barrera cutanea comprometida como en un modelo de raton de dermatitis inducida. Los resultados in vitro demostraron que el LBms penetra a traves de los espacios del SC y restablece la estructura lipidica laminar de este tejido. Ademas, se vio que cuanto mas se acercaba la composicion del LBms a la del SC mejor funcionaba reparando la barrera. Los resultados in vivo realizados en voluntarios, demostraron un aumento de la capacidad de la piel de retener agua despues de ser tratada con el LBms, indicando que el sistema refuerza la barrera. En ratones con dermatitis inducida, el tratamiento con el LBms mejoro las lesiones cutaneas. Tambien redujo la perdida de agua transepidermica indicando una reparacion de la barrera cutanea y disminuyo la inflamacion. En conclusion, el LBms puede ser de interes para el desarrollo de nuevos tratamientos para dermatitis atopica.