Miracle-dz/UniFAR-FTUs huggingface dataset:
TASCI—transcutaneous tibial nerve stimulation in patients with acute spinal cord injury to prevent neurogenic detrusor overactivity: protocol for a nationwide, randomised, sham-controlled, double-blind clinical trial

Introduction Neurogenic lower urinary tract dysfunction (NLUTD), including neurogenic detrusor overactivity (NDO) and detrusor sphincter dyssynergia, is one of the most frequent and devastating sequelae of spinal cord injury (SCI), as it can lead to urinary incontinence and secondary damage such as renal failure. Transcutaneous tibial nerve stimulation (TTNS) is a promising, non-invasive neuromodulatory intervention that may prevent the emergence of the C-fibre evoked bladder reflexes that are thought to cause NDO. This paper presents the protocol for TTNS in acute SCI (TASCI), which will evaluate the efficacy of TTNS in preventing NDO. Furthermore, TASCI will provide insight into the mechanisms underlying TTNS, and the course of NLUTD development after SCI. Methods and analysis TASCI is a nationwide, randomised, sham-controlled, double-blind clinical trial, conducted at all four SCI centres in Switzerland. The longitudinal design includes a baseline assessment period 5–39 days after acute SCI and follow-up assessments occurring 3, 6 and 12 months after SCI. A planned 114 participants will be randomised into verum or sham TTNS groups (1:1 ratio), stratified on study centre and lower extremity motor score. TTNS is performed for 30 min/day, 5 days/week, for 6–9 weeks starting within 40 days after SCI. The primary outcome is the occurrence of NDO jeopardising the upper urinary tract at 1 year after SCI, assessed by urodynamic investigation. Secondary outcome measures assess bladder and bowel function and symptoms, sexual function, neurological structure and function, functional independence, quality of life, as well as changes in biomarkers in the urine, blood, stool and bladder tissue. Safety of TTNS is the tertiary outcome. Ethics and dissemination TASCI is approved by the Swiss Ethics Committee for Northwest/Central Switzerland, the Swiss Ethics Committee Vaud and the Swiss Ethics Committee Zürich (#2019-00074). Findings will be disseminated through peer-reviewed publications. Trial registration number NCT03965299.
Involvement of chemosensory proteins in host plant searching in the bird cherry‐oat aphid

Chemosensory systems are considered to play an important role in host plant selection in herbivorous insects. However, few studies have focused on chemosensory proteins (CSPs) for aphid host‐location mechanisms. The roles of CSPs in searching for different Poaceae species (wheat, barley, triticale, maize and sorghum) were tested in Rhopalosiphum padi, an important cereal pest. The olfactometer assays showed that R. padi responds to plant odors. Seven R. padi CSP genes were identified. Influence of aphid morph, tissue and starvation state on expression patterns of CSPs was evaluated. Expression levels of CSP1, CSP4, CSP5 and CSP6 in winged aphids were significantly higher than those in wingless ones. Transcription levels of four genes (CSP1, CSP4, CSP5 and CSP6) were relatively higher in the head with antennae, and the four genes tended to be upregulated following starvation. Silencing of three CSPs (CSP4, CSP5 and CSP6) altered aphid host‐location behavior in response to the five different host plants tested. Three volatile compounds of host plants (octanal, [E]‐2‐hexenol and linalool) have significant attraction to winged R. padi according to the four‐arm olfactometer tests. Molecular docking predicted hydrogen bonding sites which played key roles in the binding of CSP4, CSP5 and CSP6 with volatile compounds. Knockdown of CSP4 or CSP5 significantly decreased the staying time of R. padi in the arms with octanal. However, knockdown of CSP6 could not affect the response of R. padi to octanal. These results bring evidence for the involvement of three CSPs in R. padi host‐location behavior.
STPA (System - Theoretic Process Analysis) Compliance with MIL - STD - 882E and other Army Safety Standards

7. MIL-STD-882E Compliance STAMP is an embodiment of the principles and approach called System Safety that was created in the defense industry about 60 years ago to cope with the increasingly complex defense systems that were being developed. STAMP was purposely created to provide a means for implementing the principles underlying System Safety so it should not be a surprise that it and the tools built on STAMP comply with and support the tasks in MIL-STD-882. In this section, the compliance with and support for each of the tasks in MIL-STD-882E is discussed.
Predicting Peritoneal Dissemination of Gastric Cancer in the Era of Precision Medicine: Molecular Characterization and Biomarkers

Gastric cancer (GC) is a leading cause of worldwide cancer-related death. Being a highly heterogeneous disease, the current treatment of GC has been suboptimal due to the lack of subtype-dependent therapies. Peritoneal dissemination (PD) is a common pattern of GC metastasis associated with poor prognosis. Therefore, it is urgently necessary to identify patients at high risk of PD. PD is found to be associated with Lauren diffuse type GC. Molecular profiling of GC, especially diffuse type GC, has been utilized to identify molecular alterations and has given rise to various molecular classifications, shedding light on the underlying mechanism of PD and enabling identification of patients at higher PD risk. In addition, a series of diagnositc and prognostic biomarkers of PD from serum, peritoneal lavages and primary GCs have been reported. This comprehensive review summarizes findings on the multi-omic characteristics of diffuse type GC, the clinical significance of updating molecular classifications of GC in association with PD risk and research advances in PD-associated biomarkers.
The Precious Few Grams of Glucose During Exercise

As exercise intensity exceeds 65% of maximal oxygen uptake carbohydrate energy sources predominate. However, relative to the meager 4–5 g blood glucose pool size in a postabsorptive individual (0.9–1.0 g·L−1 × 5 L blood = 18–20 kcal), carbohydrate (CHO) oxidation rates of 20 kcal·min−1 can be sustained in a healthy and fit person for one hour, if not longer, all the while euglycemia is maintained. While glucose rate of appearance (i.e., production, Ra) from splanchnic sources in a postabsorptive person can rise 2–3 fold during exercise, working muscle and adipose tissue glucose uptake must be restricted while other energy substrates such as glycogen, lactate, and fatty acids are mobilized and utilized. If not for the use of alternative energy substrates hypoglycemia would occur in less than a minute during hard exercise because blood glucose disposal rate (Rd) could easily exceed glucose production (Ra) from hepatic glycogenolysis and gluconeogenesis. The goal of this paper is to present and discuss the integration of physiological, neuroendocrine, circulatory, and biochemical mechanisms necessary for maintenance of euglycemia during sustained hard physical exercise.
Brain mechanisms of insomnia: new perspectives on causes and consequences.

While insomnia is the second most common mental disorder, progress in our understanding of underlying neurobiological mechanisms has been limited. The present review addresses the definition and prevalence of insomnia and explores its subjective and objective characteristics across the 24-hour day. Subsequently, the review extensively addresses how the vulnerability to develop insomnia is affected by gene variants, early life stress and major life events and brain structure and function. Further supported by the clear mental health risks conveyed by insomnia, the integrated findings suggest that the vulnerability to develop insomnia could rather be found in brain circuits regulating emotion and arousal than in circuits involved in circadian and homeostatic sleep regulation. Finally, a testable model is presented. The model proposes that in people with a vulnerability to develop insomnia, the locus coeruleus is more sensitive to - or receives more input from - the salience network and related circuits, even during REM sleep, when it should normally be sound asleep. This vulnerability may ignite a downwards spiral of insufficient overnight adaptation to distress, resulting in accumulating hyperarousal which in turn impedes restful sleep and moreover increases the risk of other mental health adversity. Sensitized brain circuits are likely to be subjectively experienced as "sleeping with one eye open". The proposed model opens up the possibility for novel intervention studies and animal studies, thus accelerating the ignition of a neuroscience of insomnia, which is direly needed for better treatment.
Research Advances in the Effects of Environmental Factors on the Symbiotic Nitrogen Fixation of Legumes

Environmental factors are always one of the major reasons why the symbiotic nitrogen-fixing system of rhizobia and legumes cannot come into full play in agricultural production. Now the environmental factors involved with the research include water, nutrient elements; temperature, heavy metals, sodium salts, carbon dioxide, soil types and pH, etc. water stress can result in decreased root nodules and decreased nitrogen-fixation efficiency; besides nitrogen, phosphorous and potassium, trace elements remarkably affect nitrogen fixation; improper temperature can limit the nitrogen fixation by legumes to a certain extent; heavy metals could directly and indirectly affect the symbiotic nitrogen fixation in many manners and thus it is a research hotspot to find some pioneering gangue-colonizing legumes . The paper details the research in this respect as well as briefly analyzing the major current research problems and trends of this respect at home and abroad.
Convalescent Plasma Therapy for COVID-19: State of the Art

Convalescent plasma (CP) therapy has been used since the early 1900s to treat emerging infectious diseases; its efficacy was later associated with the evidence that polyclonal neutralizing antibodies can reduce the duration of viremia. Recent large outbreaks of viral diseases for which effective antivirals or vaccines are still lacking has renewed the interest in CP as a life-saving treatment. The ongoing COVID-19 pandemic has led to the scaling up of CP therapy to unprecedented levels. Compared with historical usage, pathogen reduction technologies have now added an extra layer of safety to the use of CP, and new manufacturing approaches are being explored. SUMMARY Convalescent plasma (CP) therapy has been used since the early 1900s to treat emerging infectious diseases; its efficacy was later associated with the evidence that polyclonal neutralizing antibodies can reduce the duration of viremia. Recent large outbreaks of viral diseases for which effective antivirals or vaccines are still lacking has renewed the interest in CP as a life-saving treatment. The ongoing COVID-19 pandemic has led to the scaling up of CP therapy to unprecedented levels. Compared with historical usage, pathogen reduction technologies have now added an extra layer of safety to the use of CP, and new manufacturing approaches are being explored. This review summarizes historical settings of application, with a focus on betacoronaviruses, and surveys current approaches for donor selection and CP collection, pooling technologies, pathogen inactivation systems, and banking of CP. We additionally list the ongoing registered clinical trials for CP throughout the world and discuss the trial results published thus far.
Effect of lobaplatin on apoptosis of breast cancer MCF-7 cells and expression of MTDH gene

Objective To investigate the effect of lobaplatin on the apoptosis of MCF-7cells and the influence on MTDH gene expression.Methods MTT assay and flow cytometry were used to detect the effect of lobaplatin on the proliferation and apoptosis of MCF-7 cells.RT-PCR assay was used to detect the effect of lobaplatin on MTDH mRNA expression.Results 5-40mg/L lobaplatin could inhibit the proliferation of MCF-7 cells significantly,with the tendency of concentration-time-dependence(P0.05). The apoptosis rates of MCF-7 cells treated with 5mg/L,10mg/L and 15mg/L lobaplatin were 19.08%,24.23% and 65.71%,respectively,which showed obvious dose-dependence.The expression of MTDH mRNA could be inhibited by certain concentration of lobaplatin and no more MTDH mRNA could be detected with more than 10mg/L lobaplatin.Conclusion Lobaplatin could inhibit the proliferation of MCF-7 cells and induce apoptosis.Lobaplatin may be affect the migration and invasion of breast cancer cells by inhibiting MTDH gene expression.
The Relationship between Maternal Personality Disorder and Early Birth Outcomes: A Systematic Review and Meta-Analysis

(1) Background: Women with personality disorder are at risk of social and emotional problems which impact deleteriously on everyday functioning. Moreover, a personality disorder diagnosis has been established to have an adverse impact upon pregnancy outcomes and child health. Understanding this impact is critical to improving both maternal and child outcomes. This systematic review and meta-analysis will evaluate the contemporary evidence regarding these relationships. (2) Methods: Prospero and Cochrane were searched for any systematic reviews already completed on this topic. Academic Search Premier, CINAHL Complete, MEDLINE, PsycARTICLES, PsycINFO via the EBSCO host, and the Web of Science Core Collection were searched to include research articles published between 1980 and 2019. A total of 158 records were identified; 105 records were screened by reviewing the abstract; 99 records were excluded; 6 full text articles were assessed for eligibility; 5 records were included in the review. (3) Results: All the included studies reported on preterm birth. The meta-analysis indicates significant risk of preterm birth in women with personality disorder (overall odds ratio (OR) 2.62; CI 2.24–3.06; p < 0.01). Three studies reported on low birth weight, with the meta-analysis indicating a raised risk of low birth weight of the babies born to women with personality disorder (overall OR 2.00 CI 1.12–3.57 (p = 0.02)). Three studies reported on appearance, pulse, grimace, activity, and respiration (APGAR) score, with the meta-analysis of OR’s indicating a risk of low APGAR score in women with personality disorder (overall OR 2.31; CI 1.17–4.55; p = 0.02). (4) Conclusions: The infants of women with personality disorder are at elevated risk of preterm birth, low birth weight and low APGAR score.
Response of Soil Respiration and Its Components to Warming and Dominant Species Removal along an Elevation Gradient in Alpine Meadow of the Qinghai-Tibetan Plateau.

The Qinghai-Tibetan Plateau is experiencing unprecedented temperature rises and changes in plant community composition owing to global warming. Few studies focused on the combined effects of warming and changes in species composition on soil respiration (Rs). We conducted a 4-year experiment (2015-2018) to examine the influences of warming and dominant plant species removal on Rs and its autotrophic (Ra) and heterotrophic (Rh) components, along an elevation gradient (3200, 3700, and 4000 m) for alpine meadow of the Qinghai-Tibetan Plateau. Results showed that warming positively affected Rs, and the stimulation of Rs gradually diminished at 3200 m but remained stable at 3700 m and 4000 m as warming progressed. Warming did not influence Ra at all sites. Dominant species removal produced hysteretic behavior that decreased Ra (29%) at 3700 m but increased Ra (55%) at 4000 m in 2018. No significant effect of dominant species removal on Rh was observed. Significant interactive effects of warming and dominant species removal were detected only on Ra at 3700 and 4000 m. Accordingly, under future warming, soil organic matter decomposition at higher elevation will enhance positive feedback to atmospheric CO2 concentration more than at lower elevation, thus accelerating soil organic carbon loss.
Molecular Physiology of Bile Acid Signaling in Health, Disease and Aging.

Over the last two decades, bile acids (BAs) have become established as important signaling molecules that enable fine-tuned inter-tissue communication from the liver, their site of production, over the intestine, where they are modified by the gut microbiota, to virtually any organ, where they exert their pleiotropic physiological effects. The chemical variety of BAs, to a large extent determined by the gut microbiome, also allows for a complex fine-tuning of adaptive responses in our body. This review provides an overview of the mechanisms by which BA receptors coordinate several aspects of physiology and highlights new therapeutic strategies for diseases underlying pathological BA signaling.
The influence of selenium on Pro and MDA contents of rape seedling stage

Applies soil cultured and hydrophically grown of combination manner in pots ,designs different concentration selenium liquid to treat and culture rape,analyzes Pro and MDA content of rape seedling,The results are as follows,different treatment Pro content:12μg Se·ml -1 15μg Se·ml -1 18μg Se·ml -1 21μg Se·ml -1 24μg Se·ml -1 9μg Se·ml -1 control,and MDA content,24μg Se·ml -1 21μg Se·ml -1 control15μg Se·ml -1 18μg Se·ml -1 12μg Se·ml -1 9μg Se·ml -1 The proper application of selenium can decrease MDA content but high Se concentration can increase MDA content,adding selenium can raise Pro content,enhance hardiness of rape seedling
Theoretical And Practical Considerations Regarding The Importance Of Investment In Technology And Information In The Process Of Economic Growth

The main purpose of this study is to capture ,based on new theories of economic growth in the knowledgebased economic development, the importance of investing in information are as a new factor of production, we propose a different treatment of essential identifiable knowledge structures: technologies and information . Meanwhile, the article aims to capture the role that information plays in this highly complex process of economic growth. I realized through this expose, a representation of specific investments in information behavior and reasoning phenomena sustainable development concepts.
Molecular Dynamics Simulations on Interindividual Variability of Intestinal Fluids: Impact on Drug Solubilization

Efficient delivery of oral drugs is dependent on their solubility in human intestinal fluid, a complex and dynamic fluid that contains colloidal structures composed of small molecules. These structures solubilize poorly water-soluble compounds, increasing their apparent solubility, and possibly their bioavailability. In this study, we conducted coarse-grained molecular dynamics simulations with data from duodenal fluid samples previously acquired from five healthy volunteers. In these simulations, we observed the self-assembly of mixed micelles of bile salts, phospholipids, and free fatty acids. The micelles were ellipsoids with a size range of 4–7 nm. Next, we investigated micelle affinities of three model drugs. The affinities in our simulation showed the same trend as literature values for the solubility enhancement of drugs in human intestinal fluids. This type of simulations is useful for studies of events and interactions taking place in the small intestinal fluid.
Conformational dynamics of the periplasmic chaperone SurA.

The periplasmic protein SurA is the primary chaperone involved in the biogenesis of bacterial outer membrane proteins (OMPs) and is a potential antibacterial drug target. The three-dimensional structure of SurA can be divided into three parts, a core module formed by the N- and C- terminal regions, and two peptidyl-prolyl isomerase (PPIase) domains P1 and P2. Despite the determination of several SurA-peptide complex structures, the functional mechanism of this chaperone remains elusive and the roles of the two PPIase domains are yet unclear. Herein, we characterize the conformational dynamics of SurA by using solution NMR and single-molecule fluorescence resonance energy transfer (smFRET) methods. We demonstrate a "closed-to-open" structural transition of the P1 domain that is correlated with both chaperone activity and peptide binding, and show that the flexible P2 domain can also occupy conformations that closely contact the NC core module. Our results offer the structural basis for the counteracting roles of the two PPIase domains in regulating the SurA chaperone activity.
W:Ti Flexible Transversal Electrode Array for Peripheral Nerve Stimulation: A Feasibility Study

The development of hardware for neural interfacing remains a technical challenge. We introduce a flexible, transversal intraneural tungsten:titanium electrode array for acute studies. We characterize the electrochemical properties of this new combination of tungsten and titanium using cyclic voltammetry and electrochemical impedance spectroscopy. With an in-vivo rodent study, we show that the stimulation of peripheral nerves with this electrode array is possible and that more than half of the electrode contacts can yield a stimulation selectivity index of 0.75 or higher at low stimulation currents. This feasibility study paves the way for the development of future cost-effective and easy-to-fabricate neural interfacing electrodes for acute settings, which ultimately can inform the development of technologies that enable bi-directional communication with the human nervous system.
Complex Variant Discovery Using Discordant Cluster Normalization

Complex genomic structural variants (CGSVs) are abnormalities that present with three or more breakpoints, making their discovery a challenge. The majority of existing algorithms for structural variant detection are only designed to find simple structural variants (SSVs) such as deletions and inversions; they fail to find more complex events such as deletion-inversions or deletion-duplications, for example. In this study, we present an algorithm named CleanBreak that employs a clique partitioning graph-based strategy to identify collections of SSV clusters and then subsequently identifies overlapping SSV clusters to examine the search space of possible CGSVs, choosing the one that is most concordant with local read depth. We evaluated CleanBreak's performance on whole genome simulated data and a real data set from the 1000 Genomes Project. We also compared CleanBreak with another algorithm for CGSV discovery. The results demonstrate CleanBreak's utility as an effective method to discover CGSVs.
Zinc Therapy in Early Alzheimer’s Disease: Safety and Potential Therapeutic Efficacy

Zinc therapy is normally utilized for treatment of Wilson disease (WD), an inherited condition that is characterized by increased levels of non-ceruloplasmin bound (‘free’) copper in serum and urine. A subset of patients with Alzheimer’s disease (AD) or its prodromal form, known as Mild Cognitive Impairment (MCI), fail to maintain a normal copper metabolic balance and exhibit higher than normal values of non-ceruloplasmin copper. Zinc’s action mechanism involves the induction of intestinal cell metallothionein, which blocks copper absorption from the intestinal tract, thus restoring physiological levels of non-ceruloplasmin copper in the body. On this basis, it is employed in WD. Zinc therapy has shown potential beneficial effects in preliminary AD clinical trials, even though the studies have missed their primary endpoints, since they have study design and other important weaknesses. Nevertheless, in the studied AD patients, zinc effectively decreased non-ceruloplasmin copper levels and showed potential for improved cognitive performances with no major side effects. This review discusses zinc therapy safety and the potential therapeutic effects that might be expected on a subset of individuals showing both cognitive complaints and signs of copper imbalance.