Miracle-dz/UniFAR-FTUs huggingface dataset:
Species-Specific Drug Action in Schistosome Parasites Genetic and Molecular Basis of Drug Resistance and

, 1385 (2013); 342 Science et al. Claudia L. L. Valentim Species-Specific Drug Action in Schistosome Parasites Genetic and Molecular Basis of Drug Resistance and This copy is for your personal, non-commercial use only. clicking here. colleagues, clients, or customers by , you can order high-quality copies for your If you wish to distribute this article to others here. following the guidelines can be obtained by Permission to republish or repurpose articles or portions of articles ): January 3, 2014 www.sciencemag. org (this information is current as of The following resources related to this article are available online at http://www.sciencemag. org/content/342/6164/1385.full. html version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag. org/content/suppl/2013/11/20/science.1243106. DC1.html can be found at: Supporting Online Material http://www.sciencemag. org/content/342/6164/1385.full. html#ref-list-1 , 12 of which can be accessed free: cites 55 articles This article
A RETURN LOSS IMPROVEMENT OF SLOTTED SQUARE MICROSTRIP INSET-FEED PATCH ANTENNA

A simple square microstrip inset-feed patch antenna with slots for 2.4 GHz of WLAN applications is presented. Our proposed antenna design is a high gain, bandwidth, very low return loss and single band frequency. The antenna is capable to realize return loss (S11) of -45 dB and voltage standing wave ratio (VSWR) equals (1). The antenna can support WLAN applications at 2.4 GHz resonant frequency with bandwidth of 76.278 MHz. The antenna is excited by one port with microstrip inset feed line mechanism. The designed antenna is printed on Flame Retardant 4 (FR-4) substrate with 4.6 dielectric constant. The conducting patch and ground antenna material is copper (annealed). The square microstrip patch antenna has dimensions of 28.45 x 28.45 x 0.035 mm3 of patch plane. The omnidirectional radiation pattern of our antenna is 6.25 dBi. The proposed antenna has been simulated and analyzed through CST microwave studio (MWS) version 2018. The simulation results show that our proposed antenna is considered as a greet solution with an excellent performance to meet the requirements of various modern communication systems.
BreathEasy: Assessing Respiratory Diseases Using Mobile Multimodal Sensors

Mobil respiratory assessments using commodity smartphones and smartwatches are unmet needs for patient monitoring at home. In this paper, we show the feasibility of using multimodal sensors embedded in consumer mobile devices for non-invasive, low-effort respiratory assessment. We have conducted studies with 228 chronic respiratory patients and healthy subjects, and show that our model can estimate respiratory rate with mean absolute error (MAE) 0.72$\pm$0.62 breath per minute and differentiate respiratory patients from healthy subjects with 90% recall and 76% precision when the user breathes normally by holding the device on the chest or the abdomen for a minute. Holding the device on the chest or abdomen needs significantly lower effort compared to traditional spirometry which requires a specialized device and forceful vigorous breathing. This paper shows the feasibility of developing a low-effort respiratory assessment towards making it available anywhere, anytime through users' own mobile devices.
CONSTRUCTIONS ON NON COMMUTATIVE SCHEMES

In this paper we consider two constructions in non commutative schemes. One is a gluing of two categories at a common localization and the other is a crossed product. The latter, of course, is the more important topic here. Crossed products are originally de ned and extensively used in the theory of operator algebras [1]. They not only give examples of important classes of operator algebras, but many algebras are studied by decomposing them into certain crossed products. We believe that the crossed products also play equally an important role in the theory of non commutative schemes. As an example, we de ne
Convergence of peptidergic and non-peptidergic protein markers in the human dorsal root ganglion and spinal dorsal horn

Peripheral sensory neurons are characterized by their size, molecular profiles, and physiological responses to specific stimuli. In mouse, the peptidergic and non-peptidergic subsets of nociceptors are distinct and innervate different lamina of the spinal dorsal horn. The unique molecular signature and neuroanatomical organization of these neurons supports a labeled line theory for certain types of nociceptive stimuli. However, long standing evidence supports the polymodal nature of nociceptors in many species. We have recently shown that the peptidergic marker, CGRP, and the non-peptidergic marker, P2X3R, show largely overlapping expression at the mRNA level in human dorsal root ganglion (DRG). Herein, our aim was to assess the protein distribution of nociceptor markers, including their central projections, in the human DRG and spinal cord. Using DRGs obtained from organ donors, we observed that CGRP and P2X3R were co-expressed by approximately 33% of human DRG neurons and TrpV1 was expressed in ~60% of human DRG neurons. In the dorsal spinal cord, CGRP, P2X3R, TrpV1 and Nav1.7 protein stained the entirety of lamina II, with only P2XR3 showing a gradient of expression. This was confirmed by measuring the size of the substantia gelatinosa using Hematoxylin and Eosin staining of adjacent sections. Our findings are consistent with the known polymodal nature of most primate nociceptors and indicate that the central projection patterns of nociceptors are different between mice and humans. Elucidating how human nociceptors connect to subsets of dorsal horn neurons will be important for understanding the physiological consequences of these species differences.
DNA Methyltransferase Inhibitors with Novel Chemical Scaffolds

Inhibitors of DNA methyltransferases (DNMTs) are attractive compounds for epigenetic drug discovery. They are also chemical tools to understand the biochemistry of epigenetic processes. Herein, we report five distinct inhibitors of DNMT1 characterized in enzymatic inhibition assays that did not show activity with DNMT3B. It was concluded that the dietary component theaflavin is an inhibitor of DNMT1. Two additional novel inhibitors of DNMT1 are the approved drugs glyburide and panobinostat. The DNMT1 enzymatic inhibitory activity of panobinostat, a known pan inhibitor of histone deacetylases, agrees with experimental reports of its ability to reduce DNMT1 activity in liver cancer cell lines. Molecular docking of the active compounds with DNMT1, and re-scoring with the recently developed Extended Connectivity Interaction Features approach, had an excellent agreement between the experimental IC50 values and docking scores.
Cortical synaptic diversity naturally arises with heterogeneous neural populations

Synaptic inputs onto single cortical neurons in vivo exhibit substantial functional diversity with respect to sensory-driven activity. However, it is unclear what this diversity reflects, appearing counter-productive in generating tuned responses to specific stimuli. We propose that functional diversity naturally arises if neurons extract information encoded from noisy input populations. Focusing on a single sensory variable, orientation, we construct a probabilistic decoder that estimates orientation from the responses of a realistic hypothetical input population of neurons. Analytically derived weights exhibit diversity when input populations consist of noisy, correlated, and heterogeneous neurons. Weight diversity was necessary to accurately decode orientation. Further, in silico weight diversity matched the functional heterogeneity of dendritic spines imaged in vivo. This suggests that synaptic diversity is expected when information is extracted from realistic input populations, highlighting the importance of studying weighting structures in population coding theory and consideration in pursuits of the cortical connectome.
Registration of 24-hour accelerometric rest-activity profiles and its application to human chronotypes

ABSTRACT By collecting data continuously over 24 hours, accelerometers and other wearable devices can provide novel insights into circadian rhythms and their relationship to human health. Existing approaches for analyzing diurnal patterns using these data, including the cosinor model and functional principal component analysis, have revealed and quantified population-level diurnal patterns, but considerable subject-level variability remained uncaptured in features such as wake/sleep times and activity intensity. This remaining informative variability could provide a better understanding of chronotypes, or behavioral manifestations of one’s underlying 24-hour rhythm. Curve registration, or alignment, is a technique in functional data analysis that separates “vertical” variability in activity intensity from “horizontal” variability in time-dependent markers like wake and sleep times; this data-driven approach is well-suited to studying chronotypes using accelerometer data. We develop a parametric registration framework for 24-hour accelerometric rest-activity profiles represented as dichotomized into epoch-level states of activity or rest. Specifically, we estimate subject-specific piecewise linear time-warping functions parametrized with a small set of parameters. We apply this method to data from the Baltimore Longitudinal Study of Aging and illustrate how estimated parameters give a more flexible quantification of chronotypes compared to traditional approaches.
Potential for Genetic Improvement of Resistance to Perkinsus olseni in the Manila Clam, Ruditapes philippinarum, Using DNA Parentage Assignment and Mass Spawning

The Manila clam Ruditapes philippinarum, a major cultured shellfish species, is threatened by infection with the microparasite Perkinsus olseni, whose prevalence increases with high water temperatures. Under the current trend of climate change, the already severe effects of this parasitic infection might rapidly increase the frequency of mass mortality events. Treating infectious diseases in bivalves is notoriously problematic, therefore selective breeding for resistance represents a key strategy for mitigating the negative impact of pathogens. A crucial step in initiating selective breeding is the estimation of genetic parameters for traits of interest, which relies on the ability to record parentage and accurate phenotypes in a large number of individuals. Here, to estimate the heritability of resistance against P. olseni, a field experiment mirroring conditions in industrial clam production was set up, a genomic tool was developed for parentage assignment, and parasite load was determined through quantitative PCR. A mixed-family cohort of potentially 1,479 clam families was produced in a hatchery by mass spawning of 53 dams and 57 sires. The progenies were seeded in a commercial clam production area in the Venice lagoon, Italy, where high prevalence of P. olseni had previously been reported. Growth and parasite load were monitored every month and, after 1 year, more than 1,000 individuals were collected for DNA samples and phenotype recording. A pooled sequencing approach was carried out using DNA samples from the hatchery broodstock and from a Venice lagoon clam population, providing candidate markers used to develop a 245-SNP panel. Parentage assignment for 246 F1 individuals showed sire and dam representation were high (75 and 85%, respectively), indicating a very limited risk of inbreeding. Moderate heritability (0.23 ± 0.11–0.35 ± 0.13) was estimated for growth traits (shell length, shell weight, total weight), while parasite load showed high heritability, estimated at 0.51 ± 0.20. No significant genetic correlations were found between growth-associated traits and parasite load. Overall, the preliminary results provided by this study show high potential for selecting clams resistant to parasite load. Breeding for resistance may help limit the negative effects of climate change on clam production, as the prevalence of the parasite is predicted to increase under a future scenario of higher temperatures. Finally, the limited genetic correlation between resistance and growth suggests that breeding programs could incorporate dual selection without negative interactions.
Pharmacological Activities of Psoralidin: A Comprehensive Review of the Molecular Mechanisms of Action

Analysis of the most relevant studies on the pharmacological properties and molecular mechanisms of psoralidin, a bioactive compound from the seeds of Cullen corylifolium (L.) Medik. confirmed its complex therapeutic potential. In the last years, the interest of the scientific community regarding psoralidin increased, especially after the discovery of its benefits in estrogen-related diseases and as a chemopreventive agent. Growing preclinical pieces of evidence indicate that psoralidin has anticancer, antiosteoporotic, anti-inflammatory, anti-vitiligo, antibacterial, antiviral, and antidepressant-like effects. Here, we provide a comprehensive and critical review of psoralidin on its bioavailability, pharmacological activities with focus on molecular mechanisms and cell signaling pathways. In this review, we conducted literature research on the PubMed database using the following keywords: “Psoralidin” or “therapeutic effects” or “biological activity” or “Cullen corylifolium” in order to identify relevant studies regarding PSO bioavailability and mechanisms of therapeutic effects in different diseases based on preclinical, experimental studies. In the light of psoralidin beneficial actions for human health, this paper gathers complete information on its pharmacotherapeutic effects and opens new natural therapeutic perspectives in chronic diseases.
Distinct roles of dorsal and ventral subthalamic neurons in action selection and cancellation

The subthalamic nucleus (STN) supports action selection by inhibiting all motor programs except the desired one. Recent evidence suggests that STN can also cancel an already selected action when goals change, a key aspect of cognitive control. However, there is little neurophysiological evidence for a dissociation between selecting and cancelling actions in the human STN. We recorded single neurons in the STN of humans performing a stop-signal task. Movement-related neurons suppressed their activity during successful stopping whereas stop-signal neurons activated at low-latencies regardless of behavioral outcome. In contrast, STN and motor-cortical beta-bursting occurred only later in the stopping process. Task-related neuronal properties varied by recording location from dorsolateral movement to ventromedial stop-signal tuning. Therefore, action selection and cancellation coexist in STN but are anatomically segregated. These results show that human ventromedial STN neurons carry fast stop-related signals suitable for implementing cognitive control.
Effects of Visual Locomotion and Tactile Stimuli Duration on the Emotional Dimensions of the Cutaneous Rabbit Illusion

In this study, we assessed the emotional dimensions (valence, arousal, and dominance) of the multimodal visual-cutaneous rabbit effect. Simultaneously to the tactile bursts on the forearm, visual silhouettes of saltatorial animals (rabbit, kangaroo, spider, grasshopper, frog, and flea) were projected on the left arm. Additionally, there were two locomotion conditions: taking-off and landing. The results showed that the valence dimension (happy-unhappy) was only affected by the visual stimuli with no effect of the tactile conditions nor the locomotion phases. Arousal (excited-calm) showed a significant difference for the three tactile conditions with an interaction effect with the locomotion condition. Arousal scores were higher when the taking-off condition was associated with the intermediate duration (24 ms) and when the landing condition was associated with either the shortest duration (12 ms) or the longest duration (48 ms). There was no effect for the dominance dimension. Similar to our previous results, the valence dimension seems to be highly affected by visual information reducing any effect of tactile information, while touch can modulate the arousal dimension. This can be beneficial for designing multimodal interfaces for virtual or augmented reality.
Accounting earnings management and breach of covenants: an empirical study in Brazil

Received in 10/23/2019. Ask to Revise on 10/27/2019. Resubmitted on 4/24/2020. Accepted on 6/18/2020 by Dr. José Elias Feres Almeida (Assistant Editor) and by Dr. Gerlando Augusto Sampaio Franco de Lima (Editor). Published on 9/30/2020. Organization responsible for the journal: Abracicon. Abstract Objective: This study aims to show that the Brazilian companies listed on B3, due to the imminent breach of the ratios established in the covenants, have a higher level of earnings management. Method: To identify the group of suspicious companies (close to the breach), we collected ratios of covenant breach in the notes to the financial statement, subsequently confronted with the performance indices generated based on Economática data. The analysis was developed using econometric tests, by means of multiple linear regression estimated by OLS. Results: The results point to robust evidence that suspicious Brazilian companies (close to breach) act in order to improve their outcome, possibly to avoid the breach of covenants. Contribution: this study aims to contribute to disclose the fragility of financial covenants inserted in debt contracts and, consequently, in the exposure to higher risk of third-party capital. Thus, the effect of the insertion of financial covenants would be reverse to what creditors expect.
Somatosensory evoked potentials that index lateral inhibition are modulated according to the mode of perceptual processing: comparing or combining multi-digit tactile motion

ABSTRACT Many perceptual studies focus on the brain’s capacity to discriminate between stimuli. However, our normal experience of the world also involves integrating multiple stimuli into a single perceptual event. Neural mechanisms such as lateral inhibition are believed to enhance local differences between sensory inputs from nearby regions of the receptor surface. However, this mechanism would seem dysfunctional when sensory inputs need to be combined rather than contrasted. Here, we investigated whether the brain can strategically regulate the strength of suppressive interactions that underlie lateral inhibition between finger representations in human somatosensory processing. To do this, we compared sensory processing between conditions that required either comparing or combining information. We delivered two simultaneous tactile motion trajectories to index and middle fingertips of the right hand. Participants had to either compare the directions of the two stimuli, or to combine them to form their average direction. To reveal preparatory tuning of somatosensory cortex, we used an established event-related potential design to measure the interaction between cortical representations evoked by digital nerve shocks immediately before each tactile stimulus. Consistent with previous studies, we found a clear suppression between cortical activations when participants were instructed to compare the tactile motion directions. Importantly, this suppression was significantly reduced when participants had to combine the same stimuli. These findings suggest that the brain can strategically switch between a comparative and a combinative mode of somatosensory processing, according to the perceptual goal, by preparatorily adjusting the strength of a process akin to lateral inhibition.
Easy identification of insertion sequence mobilization events in related bacterial strains with ISCompare

Motivation Bacterial genomes are composed by a core and an accessory genome. The first composed of housekeeping and essential genes, while the second is composed, in its majority, of mobile genetic elements, including transposable elements (TEs). Insertion sequences (ISs), the smallest TEs, have an important role in genome evolution, and contribute to bacterial genome plasticity and adaptability. ISs can spread in a genome, presenting different locations in nearly related strains, and producing phenotypic variations. Few tools are available which can identify differentially located ISs (DLIS) on assembled genomes. Results We developed ISCompare to profile IS mobilization events in related bacterial strains using complete or draft genome assemblies. ISCompare was validated using artificial genomes with simulated random IS insertions and real sequences, achieving the same or better results than other available tools, with the advantage that ISCompare can analyse multiple ISs at the same time and outputs a list of candidate DLIS. We think that ISCompare provides an easy and straightforward approach to look for differentially located ISs on bacterial genomes. Availability and implementation ISCompare was implemented in python3 and its source code is freely available for download at https://github.com/maurijlozano/ISCompare. Supplementary information Supplementary data are available at https://github.com/maurijlozano/ISCompare.
Beta-cell specific insulin resistance promotes glucose-stimulated insulin hypersecretion

Insulin receptor (Insr) protein can be found at higher levels in pancreatic β-cells than in most other cell types, but the consequences of β-cell insulin resistance remain enigmatic. Ins1cre allele was used to delete Insr specifically in β-cells of both female and male mice which were compared to Ins1cre-containing littermate controls at multiple ages and on multiple diets. RNA-seq of recombined β-cells revealed significant differences in multiple pathways previously implicated in insulin secretion and cellular fate, including rewired Ras and NFκB signaling. Male, but not female, βInsrKO mice had reduced oxygen consumption rate, while action potential and calcium oscillation frequencies were increased in Insr knockout β-cells from female, but not male mice. Female βInsrKO and βInsrHET mice exhibited elevated insulin release in perifusion experiments, during hyperglycemic clamps, and following i.p. glucose challenge. Deletion of Insr did not reduce β-cell mass up to 9 months of age, nor did it impair hyperglycemia-induced proliferation. Based on our data, we adapted a mathematical model to include β-cell insulin resistance, which predicted that β-cell Insr knockout would improve glucose tolerance depending on the degree of whole-body insulin resistance. Indeed, glucose tolerance was significantly improved in female βInsrKO and βInsrHET mice when compared to controls at 9, 21 and 39 weeks. We did not observe improved glucose tolerance in adult male mice or in high fat diet-fed mice, corroborating the prediction that global insulin resistance obscures the effects of β-cell specific insulin resistance. We further validated our in vivo findings using the Ins1-CreERT transgenic line and found improved glucose tolerance 4 weeks after tamoxifen-mediated Insr deletion. Collectively, our data show that loss of β-cell Insr alone is sufficient to drive glucose-induced hyperinsulinemia, thereby improving glucose homeostasis in otherwise insulin sensitive dietary and age contexts.