Miracle-dz/UniFAR-FTUs huggingface dataset:
Contacts between the LexA repressor–or its DNA‐binding domain–and the backbone of the recA operator DNA.

Using hydroxyl radical footprinting and ethylation interference experiments, we have determined the backbone contacts made by the entire LexA repressor and its amino‐terminal fragment with the recA operator DNA. These techniques reveal essentially the same contacts between both proteins and one side of the DNA helix if one assumes that the DNA stays in the normal B‐conformation. This result is somewhat unexpected because protection of guanine bases against methylation suggested a somewhat twisted recognition surface. The backbone contacts revealed by both methods are symmetrically disposed with respect to the center of the operator, providing further evidence that the operator binds two LexA monomers. Each half‐operator contains seven interfering phosphates. These phosphates are found on both sides of the 5′‐CTGT sequence that is believed to be the principal recognition target. On the side close to the center of the operator are found two phosphates, whereas the other five are clustered on the side apart from the dyad axis. We are not aware of such an extended cluster of interfering phosphates for any other DNA‐binding protein. A quantification of the hydroxyl radical footprints allowed us to compare further the affinity of the LexA repressor for the recA operator with that of its isolated DNA binding domain. We find an only 13‐fold higher binding constant for LexA than for its amino‐terminal domain, which is in good agreement with our earlier results for the uvrA operator using a completely different binding assay.
3D-Reconstruction of Soccer Scenes

The aim of this paper is to demonstrate the 3d reconstruction of human body poses and motions in the field of sports events. The reconstruction does not require any technical hardware but uses originally broadcasted television images only. A game scene is reconstructed as a 3-dimensional model and the user can look at this model not only in slow motion, but also from arbitrary positions and viewing angles of his/her choice. He/she can navigate through the scene assuming various viewing angles and positions. To judge on a penalty decision, the user can e.g. assume the viewpoint of the referee; or after a goal, the position of the goalkeeper can be taken to see whether the view on the ball was obstructed.
Sur une méthode originale d'identification des lois de comportement hyper-élastique des élastomères

Les lois de comportement hyper-elastique derivent d'un potentiel elastique W. Il existe deux approches pour la formulation de ce potentiel. La premiere est phenomenologique, en supposant que le materiau est isotrope, la fonctionnelle W depend de trois invariants I 1 (C), I 2 (C) et I 3 (C) du tenseur de Green-Cauchy droit. La deuxieme approche est macromoleculaire: basee sur le concept de modeles de reseaux. Le potentiel depend alors du premier invariant I 1 (C). Dans les deux cas, le defi est de construire des lois de comportement tridimensionnelles pouvant predire la reponse mecanique du materiau pour tous les modes de deformation. Afin d'identifier les parametres du materiau (en choisissant un potentiel phenomenologique), Diani et Rey [4] ont montre (aux grandes deformations) qu'on peut limiter la base de donnees experimentale a deux essais (essais de traction: uniaxiale et equibiaxiale). Cependant, la mise en oeuvre des essais de traction equibiaxiale n'est pas evidente, necessitant des montages specifiques. L'objectif de ce travail est de montrer qu'il est possible d'identifier les parametres a partir d'un essai de compression, equivalent a un essai de traction equibiaxiale. On montre aussi que la methode d'optimisation influe sur les valeurs numeriques des parametres du materiau et, par consequent, la finesse du lissage des courbes experimentales est amelioree. Nous avons utilise le modele de Gent [1] tenant compte de l'extensibilite maximale des chaines. Ce modele original s'apparente au modele a huit sous-chaines de Arruda et Boyce [3] et ne necessite l'identification que de deux parametres materiau (μ et J M ).
Mass Loss Controlled Thermal Pretreatment System to Assess the Effects of Pretreatment Temperature on Organic Matter Solubilization and Methane Yield from Food Waste

The effects of thermal pretreatment (TP) on the main characteristics of food waste (FW) and its biochemical methane potential (BMP) and distribution of volatile fatty acids (VFAs) under mesophilic condition (35 ⁰C) were investigated. The TP experiments were carried out at 80 °C, 100 °C, 120 °C for 2 hour and 140 °C for 1 hour. The designed TP set-up was able to minimize the organic matter loss during the course of the pretreatments. Soluble organic fractions evaluated in terms of chemical oxygen demand (COD) and soluble protein increased linearly with pretreatment temperature. In contrast, the carbohydrate solubilization was more enhanced (30 % higher solubilization) by the TP at lower temperature (80 °C). A slight increment of soluble phenols was found, particularly for temperatures exceeding 100 °C. Thermally pretreated FW under all conditions exhibited an improved methane yield than the untreated FW, due to the increased organic matter solubilization. The highest cumulative methane yield of 442 (± 8.6) mL/gVSadded, corresponding to a 28.1 % enhancement compared to the untreated FW, was obtained with a TP at 80 °C. No significant variation in the VFAs trends were observed during the BMP tests under all investigated conditions.
Solids retention time in spherical biofilms in a biofilm airlift suspension reactor

Fluorescent microparticles were used as tracer beads to measure the dynamics of solids in spherical biofilms in a biofilm airlift suspension reactor. Attachment to, release from, and penetration into the biofilms of the tracer beads were measured. The coverage of the biofilm surface was low and the steady state particle concentration on the surface was dependent on the biofilm surface characteristics. The measured attachment rate constant was identical in both experiments and appeared to be determined by the hydrodynamic conditions in the turbulent reactor. The attachment rate was much faster than the release rate of the tracer beads and, therefore, the solidsretention time in the biofilm particle is not due to a simple reversible adsorption–desorption process. The heterogeneity of the distribution oftracer beads on different sectors on the biofilm surface decreased duringthe attachment period. Due to random detachment processes the heterogeneity of the tracer bead distribution increased during the release period
Mutagenic response of the endogenous hprt gene and lacI transgene in benzo[a]pyrene-treated Big Blue B6C3F1 mice.

Big Blue (BB) and generic B6C3F1 mice were given one to three i.p. injections of 50 mg/kg benzo[a]pyrene (B[a]P) in DMSO every other day to achieve cumulative doses of 50 to 150 mg/kg. Three weeks after treatment, the mutation frequency at the endogenous hprt gene and lacI transgene was measured in splenic T cells. Generic mice given 50, 100, and 150 mg/kg B[a]P displayed induced hprt frequencies (observed hprt frequency minus control frequency) of 5.5 +/- 1.0, 11 +/- 2.0, and 19 +/- 2.6 x 10(-6), respectively (average +/- SEM). In contrast, BB mice given 50 and 150 mg/kg B[a]P displayed induced hprt frequencies of 0.9 +/- 0.6 and 9.1 +/- 1.5 x 10(-6). 32P postlabelling revealed that the lower hprt response in BB mice correlated with lower amounts of BP-DNA adducts in spleen, liver, and lung 24 hours after B[a]P exposure. Western blot analysis of liver samples from B[a]P-treated mice suggests that the reduced adduct load in turn may be due to lower P450 1A1 levels in BB mice. The frequency of induced, nonsectored blue plaques (observed blue plaque frequency minus control frequency) in BB mice receiving 50 and 150 mg/kg B[a]P was 41 +/- 9 and 134 +/- 10 x 10(-6) (15- to 40-fold higher than the induced hprt frequency in the same treated animals). Sectored plaques were observed in both control and B[a]P groups but their frequency showed no relationship to dose (sectored frequency in all groups was approximately 20 x 10(-6)). To test whether persistent DNA adducts in the packaged lambda vector were contributing to the observed blue plaque frequency, purified lambda-LIZ DNA was treated in vitro with B[a]P diol epoxide (BPDE), packaged, and plated on E. coli lawn cells. Treatment with BPDE did not produce significant increases in homogeneous blue plaques, suggesting that the majority of mutants obtained from B[a]P-treated BB mice occurred in vivo. These results indicate that B[a]P exposure produces many more mutations at the lacI transgene than at the endogenous hprt locus.
In-flight cerebral oxygen status: continuous monitoring by near-infrared spectroscopy.

BACKGROUND Little is known about the in-flight cerebral oxygen status (COS) of fighter pilots. The purpose of this study was to evaluate the COS of fighter pilots during an F-15DJ flight. METHODS Three male F-15DJ fighter pilots volunteered to serve as test subjects during aerial gunnery training (AGT) missions. During the flight, the pilots' COS was continuously monitored from the right forehead using a NIRO-300G near-infrared spectrophotometer. This new instrument is capable of measuring the concentration changes in the brain of oxygenated hemoglobin (O2Hb), reduced hemoglobin (HHb), total hemoglobin (cHb, O2Hb + HHb), and the tissue oxygenation index (TOI, O2Hb/cHb). RESULTS Continuous changes of COS within the brain were clearly demonstrated using NIRO-300G, the changes included reductions in O2Hb, cHb, and TOI with increased +Gz, in a mirror image fashion. The maximum decreased concentration of hemoglobin during flight ranged from 12.8 to 25.6 micromol x L(-1) (of brain tissue). CONCLUSIONS We believe this study is the first monitoring of COS during F-15 actual flight. The monitoring of COS during F-15 flight showed that the effect of +Gz was to simultaneously lower blood flow in a mirror image fashion. Our results demonstrated that near-infrared spectroscopy (NIRO-300G) provides a reliable and sensitive method for the monitoring of pilots' COS during flight.
Pressure applied by the emergency/Israeli bandage.

The primary objective of the study was to determine the amount of pressure exerted by a bandage modified with a "pressure bar. " The data were collected using Emergency Bandages with and without the pressure bar. In addition to measuring the pressure under the pressure bar, other pressure sensors were used to measure the pressure being exerted to other areas under the elastic Emergency Bandage (at 90 degrees, 180 degrees, and 270 degrees), but not directly under the pressure bar to determine the effectiveness of the pressure bar bandage to apply localized pressure over a wound without applying unnecessary pressure over the other areas. Two sets of statistical tests conducted: "t-tests" assuming unequal variances from two samples and the "analysis of variance" (ANOVA), single factor. From the results, it is concluded that the Emergency Bandage pressure bar is very effective in elevating the applied pressure directly under the pressure bar while at the same time not applying unnecessary pressure over other areas covered by the bandage, which allows control of hemorrhage at the site of injury (under the pressure bar area) without having to have a full tourniquet effect. Perfusion of the capillaries of the hand and fingers were found to be adequate by observation of the fingers tips (finger nail quick) and subjective pulse measurement at the wrist (radial artery).
Association of human endogenous retroviruses with multiple sclerosis and possible interactions with herpes viruses

The hypothesis that human endogenous retroviruses (HERVs) play a role in autoimmune diseases is subject to increasing attention. HERVs represent both putative susceptibility genes and putative pathogenic viruses in the immune‐mediated neurological disease multiple sclerosis (MS). Gammaretroviral HERV sequences are found in reverse transcriptase‐positive virions produced by cultured mononuclear cells from MS patients, and they have been isolated from MS samples of plasma, serum and CSF, and characterised to some extent at the nucleotide, protein/enzyme, virion and immunogenic level.
Persistence of two Rhizobium etli inoculant strains in clay and silty loam soils

The persistence of Rhizobium etli strains CE3 and Ph 163 was studied in two soil types representing major French bean growing areas in Egypt. Clay soil from MENOUFIA and silty loam soil from ISMAILIA were planted by bean Cultivars; Bronco and Giza 6. The inoculation with strain Ph 163 in the first bean cultivation was significantly higher in nodule biomass and number; whereas, the strain CE3 was significantly higher in plant biomass accumulation (Moawad et al. 2004). The persisting inocula strains seem to perform differently in the two soils in terms of nodulation, biomass accumulation and N‐uptake by the two cultivars as compared with their performance with the first inoculation. CE3 strain persisting in the soil performed better than Ph.163 strain. The nodule occupancy by the persisting inoculant rhizobial was determined by two approaches; fluorescent antibody (FA) technique and other Rep‐PCR fingerprinting. Both techniques were close in the evaluation of persisting inoculant strains which nodulated beans in the second planting season without inoculation. The results obtained showed that both strains are good survivors in the two soils. (© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
CCR3 blockade as a new therapy for asthma

Among the inflammatory cells infiltrating the lungs of asthmatic patients, eosinophils and Th2 cells are thought to play a central role in the pathogenesis of this disease. Several studies have implicated that chemokines are prime candidates for being responsible for the selective recruitment of the leukocyte subsets found in atopic diseases. Regulated upon activation, normal T-cell-expressed and secreted (RANTES), monocyte chemoattractant protein-3 (MCP-3), MCP-4 and the eotaxins, for example, have been shown in vitro to potently induce eosinophil chemotaxis as well as initiate several other pro-inflammatory activities such as integrin activation, lipid mediator biosynthesis and degranulation. Ligand binding and chemotaxis experiments with these chemokines demonstrated that a G-protein coupled-receptor (GPCR) cloned from eosinophils, termed CCR3, was responsible for producing a chemokine selectivity profile identical to that of eosinophils. In addition, blocking CCR3 on eosinophils, with a monoclonal antibody, completely abolished eosinophil responses to these chemokines. Together these studies strongly suggest a central role for this receptor in eosinophil trafficking. CCR3 has also been found on in vitro derived Th2 cells and on T-cells co-localising with eosinophils in diseased tissue, thus revealing a possible pathogenic mechanism for T-cell recruitment into the airways. Therefore, blockade of CCR3 represents a highly attractive and innovative strategy for asthma therapy.
Positive Display of Methylated Sites: A Novel Method for the Detection of Promoter Methylation

Promoter methylation represents an important mechanism for silencing gene expression in higher eukaryotes. To study methylation of the promoter of the tumor suppressor p16INK4a, a fast and simple method was developed that, in contrast to previous studies, relies on the positive display of methylated sites (PDM). The method is based on bisulfite treatment of DNA, polymerase chain reaction (PCR)-amplification of the modified DNA and restriction digest of de novo created restriction sites to positively display DNA methylation in a background of unmethylated DNA. Since methylated as well as unmethylated DNA is amplified, information on the proportion of both is provided. Using this approach, 33 ductal invasive carcinomas, 4 normal mammary tissues, and 4 cell lines were analyzed for methylation. Methylation in the p16INK4a promoter was detected in 1 of 33 carcinomas (3%) and in 0 of 4 normal tissues. The conclusion is that PDM provides a useful tool in determining the degree and pattern of promoter methylation and is suitable to screen large series of tissue samples.
Refractoriness, habituation, and mismatch effects in the auditory event-related potential to alternating stimuli

Amplitude enhancement in the N1 component of auditory event-related potentials (ERPs) to alternately presented sounds has been referred as a typical example for the effect of release from neural refractoriness. We tested this hypothesis to see whether some other effects also contribute to this phenomenon. Two tones of different frequencies were presented singly or in pairs, and ERPs were recorded using monotonous (mnt) and alternating (alt) sequences of these stimuli. Comparison of the ‘alt–mnt’ difference waveforms recorded with single and paired stimuli supported the refractoriness hypothesis. A mismatch negativity-like wave, however, was also observed, questioning the constraint of ‘at least two consecutive standards before deviant’ presumed in most mismatch negativity studies. This paradigm made it possible to delineate the ERP components related to refractoriness and mismatch detection processes.
Bcl-xL-inhibitory BH3 mimetic ABT-737 depletes platelet calcium stores.

To the editor: Recently, several studies have been published in Blood that have examined the effect of Bcl-xL–inhibitory BH3 mimetics on platelet function and lifespan. [1][1][⇓][2]–[3][3] These reports agree that 2 related BH3 mimetics, ABT-737 and ABT-263, induce platelet apoptosis. This is
A Context-aware Service Centric Approach for Service Oriented Architectures

Evolution in the fields of telecommunication and software engineering has promoted the birth of a new generation of software architectures known as Context-Aware Service Oriented Architectures (CASOA) which are articulated on a new design and development paradigm called Context-Aware Service (CAS). However, the ambiguity of the context concept and the multiplicity of services execution contexts make CAS hard to build and show why a generic approach, in accordance with best practices of software engineering for designing such services, is necessary. This paper focuses on a CAS design approach for building CASOA. To deal with such architectures development, challenges such as context management and dynamic service adaptation have to be faced. We propose in this article a design process that exploits both of our context and CAS specifications and metamodels in order to fulfil the passage from a core service in Service Oriented Architecture (SOA) to a CAS in CASOA. This passage is satisfied across a mechanism that, inspired by the Aspect Paradigm concepts, considers the service adaptations as aspects.
Treatment of Fibrous Dysplasia of the Mandible with Radical Excision and Immediate Reconstruction: Case Report

Monostotic fibrous dysplasia of the mandible is an unusual manifestation of the disease that is usually benign, occurs in young individuals, and is managed by conservative curettage or debridement. We present a case of persistent fibrous dysplasia complicated by pain and intraoral bony exposure that was successfully managed by radical resection and reconstruction with a free fibular flap. Although mandibular fibrous dysplasia is preferentially managed conservatively, treatment of this disease has evolved to a point where total excision and immediate reconstruction may be the treatment of choice and offer the best outcome.
Peirce on logical diagrams

L'A. se propose d'etablir les veritables motivations qui ont amene C. S. Peirce a modifier les diagrammes de Euler et Venn dans le volume IV de ses «Collected papers». Comparant les formules de Boole avec son systeme des graphiques existentiels, l'A. montre que Peirce definit les regles syntaxiques de transformation de diagramme en diagramme qui regissent le raisonnement relationnel
Effect of realistic MIMO channels correlation on the MIMO indoor systems detectors

A realistic stochastic multiple-input multiple-output (MIMO) channel is presented in this paper. It is shown that the spatial correlation depends on the statistical properties of the channel, the antenna pattern, the inter-element spacing and the array configuration. Furthermore, the total spatial correlation matrix is equal to the Kronecker product of the transmit and receive correlation matrices. In this paper, the effect of correlation on the MIMO system performance using zero forcing and minimum mean squared error detectors is also investigated. It is shown that an increase in channel correlation between the transmission and paths results in a decrease in SNR at the receiver, thus in a performance loss.
Microinvasive Glaucoma Surgery

Aim: The aim of the article is to discuss issues concerning microinvasive glaucoma surgery. Material and Methodology, Discussion: The article presents definitions of microinvasive procedures and their classification, and the most important types of implants as well as their implantation technique and their mechanism of action. The article also contains the most important reports concerning the indications and results of studies presented in the literature. Conclusions: Microinvasive techniques are based on physiological aspects of aqueous humor flow in the eye and their role in the glaucoma treatment algorithm is yet to be determined.
The impact of climate change on food security and agricultural production in the Pak Phanang River Basin, Thailand

The Pak Phanang River Basin used to be an important rice growing area in southern Thailand. However, the environmental changes in the area decreased the rice planting there because of a lack of fresh water and salt water intrusion. This area was selected as one of the King of Thailand's initiative projects in 1978 to restore land fertility, improve food security, and increase the quality of the life of local people. The basin consists of three provinces covering 13 districts (amphoe): Pak Phanang, Chian Yai, Chaloem Phra Kiat, Hua Sai, Cha-uat, Ron Phibun, Chulabhorn, Lan Saka, Phra Phrom and Amphoe Mueang Nakhon Si Thammarat in Nakhon Si Thammarat Province, Khuan Khanun and Pa Phayom in Phatthalung Province, and Ranot in Songkhla Province. The total area of the basin is approximately 304,000 ha, including 80,000 ha of paddy fields. The total population of the area is about 1.2 million people. The objectives of the research were to study the impact of climate change on rice production in the basin, to find suitable areas for rice production, and to discuss food security for the area. It was found that the concrete impact of the El Niño and La Niña phenomena on rice production in the study area was not clear. The study of the rice consumption in the basin showed that the demand for milled rice for local consumption was approximately 114,005.63 tons per year, derived from 172,735.80 tons of unhusked rice or raw rice. The study of land suitability for paddy rice showed that two levels of land suitability were found, including high and medium suitability. The estimation of the production capability for unhusked rice was 182,516.37 tons per year. It was found that the food security regarding rice was low because the yearly difference between production and consumption was only 9,780.57 tons, which could be used in less than one month as reserve consumption in the following year. The study of the effects of drought in the area showed that the higher elevations indicated higher drought risk because the loss of water at higher elevations was faster than at lower elevations in terms of water drainage and evaporation processes. Five levels of drought risk were classified: very high risk (elevation higher than 20 m), high risk (elevation between 16–20 m), moderate risk (elevation between 11–15 m), low risk (elevation between 6–10 m), and very low risk (elevation less than 6 m), which covered the areas of 0.06%, 0.27%, 3.57%, 13.23%, and 82.87%, respectively.