Miracle-dz/UniFAR-FTUs huggingface dataset:
MiCroBioloGiCal and MoleCular eValuation of MONILIOPHTHORA PERNICIOSA (aGariCales: MarasMiaCeae)

Moniliophthora perniciosa presents discrepancies in its taxonomy and is the causal agent of cocoa witches’ broom, which in Colombia is the second disease after moniliasis (Moniliophthora roreri). In this study 12 samples of the pathogen obtained from different cocoa materials from the Casa Luker S.A. Farm (Palestina, Caldas, Colombia) were analyzed. The DNA was obtained from monosporic cultures and from the fungus fructification. Each sample was evaluated from microbiological cultures using molecular markers ITS (ITS1, ITS2 and 5.8S genes). The Molecular ADN characterization obtained from direct extraction from the fungus fructification avoids artificial selection in the culture means and ensures a more realistic diagnosis of the pathogen diversity in the plantations. The molecular characterization allowed differentiation between M. pernicious and M. roreri.
Shadow IT - Systematic Literature Review

Shadow IT coexists with mandated information systems. Developed and applied by non­IT domain experts, it is as a rule, but not exclusively, used for resolving non­routine issues, for which mandated IS does not have appropriate functions. Shadow IT solutions are not supported or controlled by IT departments. The aim of the paper is to show to which extent shadow IT has been researched, as this area has not yet received the necessary attention from both academia and business. A systematic literature review was conducted in order to find the papers covering the topic of shadow IT. The following electronic databases were searched: Web of Science (Thomson Reuters), Scopus (Elsevier), and AIS Electronic Library (The Association for Information Systems (AIS)). More than half of the analysed papers are case studies in one or more organizations. The papers were published mostly in scientific conferences and in scientific journals. Based on the literature review, the reasons for shadow IT occurrence, the possibility of its management, as well as its advantages and disadvantages are presented. The most important conclusions and views regarding shadow IT, according to the authors’ opinion, are indicated at the end. The limitation of a systematic literature review is that only papers published in English are analysed. Besides identifying papers covering the shadow IT topics, the reasons for the emergence of shadow IT, the possibilities of its management and its advantages and disadvantages are systematized.
Optimization of Neuron cells Maturation and Differentiation

Neurodegenerative disease is the brain and spinal cord attacks to turn off his cell. One of the diseases that prevalence of HAD neurodegenerative is now increased by approximately 37%. The purpose of this study was to determine the optimal time of growth of cultured brain neurons in rats (Rattus norvegicus) and know the stages of morphological differentiation of rat brain neurons. Isolate of neuron cells was provided from fetal rat neurons, aged 18-19 days, and then cells grown in vitro on MEM+10%FBS+3%Penstrep culture medium. Cells growth observed once each day during 14 days. The results obtained are optimal neuronal cell growth are on the eighth day. Morphological differentiation of neuronal cells began on day 1 of the form neuroblast cell morphology apolar (rounded), the 2nd day of neuronal cell morphology in the form of bipolar neuroblast (already formed axon and dendrites), on day 3 to day 14 cells already mature, with cell morphology namely bipolar neurons, pyramidal and multipolar. Mature neuron cells that have optimizations on the 10th day.
The Atlas of Mouse Development

Introductory Remarks. Methodology. General Observations on the Staging of Postimplantation Mouse Embryos. General Observations on the Plates. Embryogenesis. Assessment of Developmental Stage of Pre- and Postimplantation Mouse Embryos Based on the Staging System of Theiler (1989): Preimplantation Period. Stages 1-5. Postimplantation Period. Stages 6-26. Scanning Electron Micrographs, and Special Systems: External Appearance of Intact Embryos Isolated between 7.5-9.5 Days p.c. (Text Associated with Plate 42). External Appearance of Intact Embryos Isolated between 9.5-11.5 Days p.c. , and Differentiation of the Limbs over the Period from 9.5-117.5 Days p.c. (Text Associated with Plates 43-145). External Appearance of Intact Embryos Isolated between 11.5-118.5 Days p.c. (Text Associated with Plate 46). Differentiation of the Cephalic Region in Embryos Isolated between 9.5-111.5 Days p.c. --Scanning Electron Microscopy Series (Text Associated with Plate 47). Differentiation of the Eye--Scanning Electron Microscopy and Histological Series (Text Associated with Plates 48-153). Differentiation of the Pituitary (Text Associated with Plates 54-157). Development of the Tongue (Text Associated with Plate 58). Closure of the Palate (Text Associated with Plates 59-162). Morphology of the Spinal Cord (Text Associated with Plate 63). Early Stages in the Development of the Heart (Text Associated with Plates 64 and 65). Differentiation of the Lungs (Text Associated with Plate 66). Histochemical Demonstration of Primordial Germ Cells (Text Associated with Plate 67). Differentiation of the Urogenital Ridges, Gonads, and Genital Duct System (Text Associated with Plates 68-171). Differentiation of the Kidney (Text Associated with Plate 72). Discontinuities Occasionally Found in the Visceral Yolk Sac (Text Associated with Plates 73 and Plate 74, a-1f). Differentiation of the Placenta (Text Associated with Plate 74, g-1i and Plate 75). Disposition of the Extra-embryonic Membranes Associated with Various Types of Twinning (Text Associated with Plates 76-179). Asynchronous Implantation (Text Associated with Plate 80). Embryo Code Numbers for Serially-Sectioned Embryos Illustrated in Plates 1-141. Chapter References.
Comparison of Glottal Closure Instants Detection Algorithms for Emotional Speech

In production of voiced speech, epochs or glottal closure instants (GCIs) refer to the instants of significant excitation of the vocal tract. Extraction of GCIs is used as a pre-processing stage in many areas of speech technology, such as in prosody modification, speech synthesis and voice source analysis. In the past decades, several GCI detection algorithms have been developed and most of them provide excellent results for speech signals produced using modal (normal) type of phonation. There are, however, no studies comparing multiple state-of-the-art GCI detection methods in emotional speech. In this paper, we compare six GCI detection algorithms using emotional speech and known evaluation metrics. We use the Berlin EMO-DB acted emotional speech database which contains seven emotions and simultaneous electroglottography (EGG) recordings as ground truth. The results show that all six GCI detection algorithms give best performance in processing speech of neutral emotion and that the performance degrade particularly in emotions of high arousal (anger and joy). To improve the performance of GCI detection in emotional speech, the study underlines the importance of local average pitch period estimates.
Theory of Mind, pragmatics and the brain

Abstract Theory of Mind (ToM) is a neurocognitive system that allows the perceiver to attribute mental states, such as intentions, beliefs, or feelings, to others’ actions. The aim of the present work is to analyse the engagement of the ToM system in communication, in particular, in communicative intention processing. To this aim, we propose an Intention Processing Network (IPN) with its own principles and mechanisms, that is, a brain network differentially engaged according to the complex intertwining of the context, goal, and action involved. According to our IPN model, a set of brain regions of the ToM system (i.e. left and right temporoparietal junction, precuneus, and medial prefrontal cortex) are differentially involved in comprehending different types of intention, such as private or social intentions. We provide independent and convergent evidence on the role of the IPN model in communicative intention processing and we show that the engagement of the IPN does not depend upon the communicative means used, that is, written language, auditory language, or gesture. Evidence deriving from different experimental paradigms, including neuroimaging, lesion, neurodegenerative, and brain stimulation studies are discussed. In our view, this evidence establishes a link between ToM and pragmatics studies and suggests the role of intention processing as a core feature of human communication.
Potential and limits of game-based learning

Because educational games increasingly find their way into the classroom, we aim at outlining potentials but also limitations of game-based learning. First, we compile widely acknowledged benefits, like the ability of learning games to engage and meet the learner's situation. Moreover, we argue that the reduced use of games for learning in higher education does not seem warranted. We propose a three-dimensional framework that highlights the potential of adaptive game-based learning to catalyse the learning process by addressing cognitive, emotional and social factors of the learner and characteristics of the respective learning environment. We emphasise mandatory considerations like the inability of 'serious games' to replace but complement and enhance traditional educational approaches as well as the misunderstanding that the current generation has a 'native' understanding of technology. With adequate resources, theoretical frameworks, interdisciplinary competencies and clear goals, one can realise effective game-based learning that meets the requirements of modern education.
FORMAÇÃO EM GESTÃO PARA APOIO À REFORMA DA ATENÇÃO PRIMÁRIA À SAÚDE EM PORTUGAL E PAÍSES AFRICANOS LUSÓFONOS

Resumo Apresentamos lições que resultaram de atividades de capacitação dos gestores conduzidas em Portugal no contexto da reforma da atenção primária em saúde e nos países africanos de língua oficial portuguesa, em termos do planejamento e da gestão dos serviços hospitalares e de saúde pública. Descrevemos três programas de formação-ação realizados pela Unidade de Saúde Pública Internacional do Instituto de Higiene e Medicina Tropical de Lisboa, com o apoio de parceiros portugueses e internacionais como a Organização Mundial da Saúde e o Instituto de Medicina Social da Universidade do Estado do Rio de Janeiro. Os programas foram desenvolvidos na base da identificação das necessidades de competências dos participantes e focaram a resolução de problemas concretos com o objetivo de ajudar os gestores a enfrentar as dificuldades inerentes aos processos de reforma. Apesar do seu valor intrínseco, por si só não se mostram suficientes, uma vez que são sempre necessários outros mecanismos, como o acompanhamento continuado dos gestores, sistemas de incentivos coerentes com os objetivos das reformas, ferramentas e recursos (financiamento, sistemas de informação, pessoal qualificado suficiente) adequados para implementar as mudanças. Além disso, a sustentabilidade das intervenções de fortalecimento das capacidades carece de apoio continuado dos decisores políticos.
The Raw and the Stolen

Cooking is a human universal that must have had widespread effects on the nutrition, ecology, and social relationships of the species that invented it. The location and timing of its origins are unknown, but it should have left strong signals in the fossil record. We suggest that such signals are detectable at ca. 1.9 million years ago in the reduced digestive effort (e.g., smaller teeth) and increased supply of food energy (e.g., larger female body mass) of early Homo erectus. The adoption of cooking required delay of the consumption of food while it was accumulated and/or brought to a processing area, and accumulations of food were valuable and stealable. Dominant (e.g., larger) individuals (typically male) were therefore able to scrounge from subordinate (e.g., smaller) individuals (typically female) instead of relying on their own foraging efforts. Because female fitness is limited by access to resources (particularly energetic resources), this dynamic would have favored females able to minimize losses to theft. To do so, we suggest, females formed protective relationships with male co‐defenders. Males would have varied in their ability or willingness to engage effectively in this relationship, so females would have competed for the best food guards, partly by extending their period of sexual attractiveness. This would have increased the numbers of matings per pregnancy, reducing the intensity of male intrasexual competition. Consequently, there was reduced selection for males to be relatively large. This scenario is supported by the fossil record, which indicates that the relative body size of males fell only once in hominid evolution, around the time when H. erectus evolved. Therefore we suggest that cooking was responsible for the evolution of the unusual human social system in which pair bonds are embedded within multifemale, multimale communities and supported by strong mutual and frequently conflicting sexual interest.
An Energy Storage System for Recycling Regenerative Braking Energy in High-Speed Railway

This paper proposes an energy storage system (ESS) for recycling the regenerative braking energy in the high-speed railway. In this case, a supercapacitor-based storage system is integrated at the DC bus of the back to back converter that is connected to the two power phases of the traction power system (TPS). In order to ensure the suitability of the ESS in the TPS, the operation modes are classified by considering the load conditions of the TPS and the state-of-charge limit of the supercapacitor. Then, a master-slave control strategy with a central controller is proposed. In which, the central controller realizes the flexible management for all operation modes, by means of the state machine logic. Meanwhile, the master-slave control serves for coordinating the operation of the multiple converters according to the commands from the central controller. Finally, the capabilities of the proposed ESS are validated by sufficient experiments under different operation modes and the simulation based on the field data.
An interactive system for humanoid robot SHFR-III

The natural interaction between human and robot is full of challenges but indispensable. In this article, a human–robot interactive system is designed for humanoid robot SHFR-III. The system consists of three subsystems: multi-sensor positioning subsystem, emotional interaction subsystem, and dialogue subsystem. The multi-sensor positioning subsystem is designed to improve the positioning accuracy, the emotional interaction subsystem uses bimodal emotional recognition model and fuzzy emotional decision-making model to realize the emotion recognition and expression feedback to the interactive objects, and the dialogue subsystem with personal information can complete the response consistent with the default information and avoid conflicting responses . The experimental results show that the multi-sensor positioning subsystem has good environmental adaptability and positioning accuracy, the emotional interaction subsystem can achieve human-like emotional feedback, and the dialogue subsystem can achieve more natural, logical, and consistent responses.
Applicazione di tecniche isotopiche per valutare la risposta funzionale di cedui mediterranei al taglio di avviamento all’alto fusto

Abstract: Discrimination of stable isotopes of carbon and hydrogen provides an effective tool for interpreting time-integrated responses of plants to environmental conditions and delineate sources of plant water uptake. In this work, isotopic analyses were carried out in two Mediterranean oak forests in which a thinning experiment on replicated plots has been performed. Changes in carbon isotope discrimination suggests an increase of water use efficiency soon after thinning. Together with changes in the hydrogen isotopic composition in xylem sap, this may suggest that trees are able of a rather prompt physiological acclimation to cope effectively to new environmental conditions and changes in resource availability.
Power control method for improving efficiency of laser‐based wireless power transmission system

Laser-based wireless power transmission (LWPT) systems are one of the most promising systems in the long-range wireless power transfer field. However, few studies have focused on the efficiency characteristics of LWPT systems. Therefore, most LWPT systems are open-loop systems, making it difficult to best utilise their lasers and photovoltaic cells, thus preventing the system efficiency from being further improved. In this study, a closed-loop power control method is proposed for LWPT systems to explore the best performance conditions for such systems. To this end, the efficiency characteristics of a system are investigated by means of theoretical analysis and experimental measurements. The results show that the duty cycle of the laser input current is one of the key factors for improving the system efficiency. Then, a dual closed-loop controller, which includes an optimal duty cycle search algorithm, is proposed based on the obtained system characteristics. Finally, a small-scale prototype is fabricated and tested in the laboratory to verify the effectiveness of the proposed power control method.
Girişimcilerin Gözünden Girişimciliği Etkileyen İçsel ve Dışsal Faktörler

Bu calismada bireylerin girisimci olmasinda rol oynayan icsel ve dissal faktorlerin neler oldugu ve bu faktorlerden hangisinin girisimcilik kariyerini secmede digerlerine ustunluk kurdugunun ortaya konulmasi amaclanmistir. Bu amac dogrultusunda nitel bir arastirma gerceklestirilmistir. Bu baglamda Turkiye’nin cesitli illerinde isyeri sahibi olan, 9’u erkek ve 7’si kadin olmak uzere toplam 16 girisimci ile derinlemesine gorusmeler yapilmistir. Gorusmelerde onceden hazirlanmis ve 10 adet yari yapilandirilmis sorudan olusan gorusme formu uygulanmistir. Elde edilen veriler tematik ag analizi yontemine gore NVivo paket programi araciligiyla analiz edilmistir. Arastirmanin bulgularina gore bireyleri girisimcilige iten unsurlar arasinda icsel faktorler ve dissal faktorler rol oynamaktadir. Fakat icsel faktorlerin daha etkili oldugu gorulmustur. Ozellikle kisilik ozelliklerinin girisimcilik kariyerini secmede en onemli unsur oldugu tespit edilmistir. Arastirma, icsel ve dissal faktorleri bir arada irdelemesi ve girisimcilik kariyerini tercih etme potansiyeli olanlardan ziyade halihazirda girisimcilik yapan bireylere odaklanmasi yonuyle diger calismalardan ayrilmaktadir. Ayrica girisimcilerin gorusleri dogrultusunda sahip olunan ideallerin ve hayallerin bireyleri girisimcilige iten birer faktor olmasinin kesfedilmesi, calismanin literature yonelik ozgun bir katki sunmasini saglamistir.
A meiosis-specific form of the Anaphase Promoting Complex/Cyclosome regulates the oocyte-to-embryo transition in Drosophila

Cell cycle transitions during mitosis and meiosis must proceed in an irreversible manner. At the heart of this is the Anaphase Promoting Complex/Cyclosome (APC/C), an E3 ubiquitin ligase. The APC/C targets its substrates for degradation, and thus progresses the cell cycle irreversibly forward. Many substrates of the APC/C have been identified in mitosis, but how the APC/C regulates meiosis is less well understood. The Drosophila gene cortex encodes a female, meiosis-specific activator of the APC/C. We set out to identify specific substrates of APCcor both genetically and biochemically. A genetic screen identified five deficiencies that suppress an arrest caused by low APCcot activity. In some cases, these deficiencies could be narrowed to regions containing only a few genes. IP/mass-spectrometry was also performed to identify interactors of Cortex. One hit was Matrimony, a potent inhibitor of Polo kinase during prophase 1. Cort and Mtrm can interact directly in vitro, while a mitotic APC/C activator, fzy/cdc20, cannot. Mtrm proteins levels are drastically reduced upon completion of meiosis, and this reduction is dependent on cort. When expressed in cell culture, Cort causes a proteasome dependent decrease in Matrimony protein levels. Cort and Mtrm also interact genetically, and overexpression of Mtrm in the early embryo causes developmental defects in a subset of embryos. This work contributes to our understanding of the meiotic cell cycle and the specific regulation that distinguishes it from mitosis. Thesis Supervisor: Terry L. Orr-Weaver Title: Professor of Biology
Pre-analytical issues in liquid biopsy – where do we stand?

Abstract It is well documented that in the chain from sample to the result in a clinical laboratory, the pre-analytical phase is the weakest and most vulnerable link. This also holds for the use and analysis of extracellular nucleic acids. In this short review, we will summarize and critically evaluate the most important steps of the pre-analytical phase, i.e. the choice of the best control population for the patients to be analyzed, the actual blood draw, the choice of tubes for blood drawing, the impact of delayed processing of blood samples, the best method for getting rid of cells and debris, the choice of matrix, i.e. plasma vs. serum vs. other body fluids, and the impact of long-term storage of cell-free liquids on the outcome. Even if the analysis of cell-free nucleic acids has already become a routine application in the area of non-invasive prenatal screening (NIPS) and in the care of cancer patients (search for resistance mutations in the EGFR gene), there are still many unresolved issues of the pre-analytical phase which need to be urgently tackled.
Catching the fly in dystopian times

A recommendation of: Pablo F. Cuervo, Alejandro Percara, Lucas Monje, Pablo M. Beldomenico, Martin A. Quiroga Environmental variables determining the distribution of an avian parasite: the case of the Philornis torquans complex (Diptera: Muscidae) in South America 10.1101/839589
Reaction kinetics of lactulose and furosine formation - influence of dilution of milk during direct UHT treatment.

Afin d'eclaircir l'effet de dilution du lait sur la cinetique de la formation de lactulose et furosine on a entrepris des etudes systematiques. On a trouve que le facteur d'Arrhenius A est fonction de la concentration initiale du lactose. Les donnees relatives a la cinetique de formation de lactulose et furosine pendant le traitement UHT sont comparees a celles obtenu et pour le chauffage indirect, en tenant compte de la dilution
Importance of Parasitic Vaccines in Integrated Control of Parasitic Diseases in Livestock

Diseases caused by parasites are responsible for most destructive and prevalent diseases in animals. Parasitic infections are related with huge economic losses in terms of production losses, losses due to infertility. Control of parasitic diseases in animals should be aimed to increase the productivity. Therefore, efficient, economic, and sustainable control methods are to be used. Despite the availability of a number of effective drugs for the treatment of most important diseases, there is need for the development of successful vaccines are not investigated. The reasons for the development of vaccines is International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 9 Number 2 (2020) Journal homepage: http://www.ijcmas. com