Miracle-dz/UniFAR-FTUs huggingface dataset:
In vitro studies on the anticancer activity of Bacopa monnieri

B. monnieri plant extract has been reported to have anticancer property. Different concentrations of ethanolic extract of B. monnieri were tested with Sarcoma-180 cell culture. The cell growth, viability and 3 H-thymidine incorporation into the DNA were assessed. Sarcoma-180 cell growth was significantly inhibited with increasing concentrations of B. monnieri. 3 H-thymidine uptake study suggested that the site of action of the drug may be at DNA replication stage. Hence, the alcoholic extract of B. monnieri can be considered as an anticancer drug.
Finding the silver linings: Psychosocial correlates of posttraumatic growth among husbands of Chinese breast cancer survivors

Caregiving does not have to be a totally negative experience for the husband caregivers of breast cancer survivors (BCS). There are growing interests in exploring the positive psychological changes (aka posttraumatic growth; PTG) among husbands of BCS. Western studies have shown that coping resources, cognitive appraisal, and coping strategies are associated with PTG among caregivers of BCS. Studies in the Chinese context are limited. This study examined the psychosocial correlates of PTG among husbands of Chinese BCS.
Chemotype Discrimination and Rapid Identification of Angelica Roots by DART-TOF-MS

The Angelica root has been used as a medicinal herb in many Asian countries including Korea, China, and Japan. Angelica gigas, A. sinensis, and A. acutiloba have been considered as Angelicae radix in Korean, Chinese, and Japanese Pharmacopoeia, respectively. Since the origins of Angelicae radix differ from country to country, there is a need to develop an efficient analytical method to identify the origin of the Angelica root. In order to obtain chemical fingerprints, three different Angelicae Radices were analyzed by direct analysis in real time mass spectrometry (DART-MS). Significantly different DART-MS spectra were observed from three different species of Angelicae Radix. Strong peaks of decursin or decusinol angelate, and its dimer were exclusively found from A. gigas. Ligustilide and linoleic acid were detected as the major component from A. acutiloba. The strongest ligustilide peak was observed from A. sinensis. DART-MS fingerprinting is a promising method for the rapid identification and/or quality control of Angelicae Radix.
Fertility Technology Research and the Use of Human Beings as Property

In January 2020, an article in the Journal of Human Reproduction exploring whether human embryos could be obtained via uterine lavage and to compare their quality to embryos created via in vitro fertilization. Any embryo that was not removed via lavage was either prevented from implanting by giving the women injections of gonadotropin releasing hormone antagonists or aborted with either methotrexate or uterine curettage. This research was done using women in Mexico, who were paid the equivalent of over two months’ wages and who signed away their rights to their embryos, including agreeing to have an abortion if implantation did occur. Not only is this another instance of human beings being treated as property but is against the dignity of these women by turning them into, as one ethicist says, “human petri dishes. ” Summary: Researchers continue to use people as objects to obtain their goals. In this case, it was poor women in Mexico and their embryos. The Editors of Journal of Human Reproduction enabled this by publishing the report.
Improving quality of care in inflammatory bowel disease: treatment targets and body composition

17 DECLARATION . 19 ACKNOWLEDGEMENTS . 21 CHAPTER 1: OVERVIEW . 23 REFERENCES . 26 CHAPTER 2: INTRODUCTION . 29 Definitions, clinical presentation, and diagnosis of IBD . 29 Definitions. 29 Clinical presentation. 29 Diagnosis. 29 IBD pathogenesis, epidemiology, natural history, classification and burden . 30 Pathogenesis. 30 Epidemiology. 30 Natural history. 31 Classification. 31 Socioeconomic burden. 31 Evaluating outcomes of care in IBD . 31 Quality of care in IBD . 32 Un-promoted areas of care in IBD . 33 Conventional management of IBD . 35 Therapeutic considerations in IBD . 35 Patient factors. 36 Disease-related factors. 36 Medication-related factors. 36 Conventional disease activity assessment in IBD . 36
Tissue Culture of Africa Violet

It was studied that the effect of ectohormone on the formation of callus and adventitious root,the differentiation of adventitious bud and the reproduction in the MS media containing 6-BA,NAA and GA were Studied with the young leaf as explant.The result showed that the callus abduction rate of per litre MS medium added 0.1-2.0mg 6-BA and 0.01-0.5mg NAA was over 20%;per litre MS medium added 0.1-1.0mg 6-BA,0.01-0.5mg NAA and 0.1-1.0mg GA was propitious to the bud growth and multiplicatio;GA could improve adventitious bud and seedling;the rooting of 1/2MS medium with NAA 0.1-0.5mg/L,IBA 0.1mg/L or IAA 0.1-0.5mg/L was over 97% within 20d;the surviving of seedling was over 90% in the culture medium,5 soil with rotten leaf and 3 perlite.
Pharmacological Action of Polysaccharides from Radix Codonopsis on Immune Function and Hematopoiesis in Mice

Objective To study the influences of polysacch arides(PS)from Radix Codonopsis on immune function and hematopoiesis in mice.Methods Several experiments have been condu cted for detecting delayed type hypersensitivity(DTH),hemolysin antibody concentration,endogenous spleen colony,3 H-TdRpermeate nuleated cells in bon e marrow.Results By oral administration,PS from Radix Codonopsis can promote the humoral immunity and PS at a small dosage en-hance the cellular immunity.PS from Radix Codonopsis can also elevate the hemoglobin cont ent of the hemolysis -induced blood -deficiency mice and increase the formation of endogenous spleen c olony,but has less effect on DNA synt hesis in the bone marrowof blood -deficiency mic e caused by 60 Co -γray. Conclusion PS from Radix Codonopsis can enhance the im-mune function and improve the compen satory hematopoiesis of spleen and t heir mechanisms need to be further studied.
Flower visiting and pollen transport by the imported cabbage butterfly (Lepidoptera: Pieridae) in a highly disturbed urban habitat

Throughout their flight season from April to November, adult imported cabbage butterflies (ICBS), Pieris rapae , visited one hybrid and 37 flower species in a large, complex urban vegetable and flower garden in Washington, D.C. Of these flower species, 92% were exotic, and the corolla depths of some of them were significantly different. Individual butterflies carried means of from 0.8 to 8.8 pollen grains of each of seven flower species on their tongues and from 0 to 1.7 grains from four flower species on their legs. They transported significantly more pollen grains of radish and Iythrum on their tongues than on their legs. Also, they carried more pollen of radish than three other flowers examined. Their constancy in species of flower visited during foraging bouts and movements of their tongues and legs against stigmas suggest that they may pollinate these flowers, but they seem to function primarily as nectar thieves.
Sexism in Schools: a new look

Research on gender differences in classroom interaction patterns is reviewed, highlighting deficiencies in the methodology, reporting and use made of the research. The analysis of 24 Year 8 science lessons is reported which indicates that the interaction patterns of girls are not significantly different from those of boys. Suggestions for future classroom interaction research in this field based on more complex perspectives than the relatively simplistic sex difference approach are proposed.
A study of the genetics and growth of a tripartite RNA virus using ts mutants

The growth characteristics of Maguari virus and Bunyamwera virus of the family Bunyaviridae were investigated using ts mutants and revertants. By genetic analysis involving recombination by segment reassortment between viruses, it was possible to assign Group I ts mutants to the S RNA, Group II to the M RNA and Group III to the L RNA segments. Representatives of each of these groups of mutants showed different phenotypes, which presumably reflect the effects of the ts lesions on the different virus functions encoded by each genome segment. It was found that Maguari virus dominates over Bunyamwera virus in mixed infections and this probably accounts for the predominance of Maguari genotype isolates from genetic crosses. Examination of a mixed reassortant clone indicated that multiple copies of at least the S RNA segment can exist in a virus preparation in a more intimate relationship than a simple mixture of two populations of viruses. Analysis of Group II revertants with altered G1 protein electrophoretic mobility provided information on the glycosylation of wild type G1 protein. It was found that the growth of these revertants in tissue culture was not defective despite the gross changes in a major structural protein. However, it was found that antiserum which neutralised the wild type virus failed to neutralise infectivity of one of the revertants.
Explanatory Style Change During Cognitive Therapy for Unipolar Depression

We administered the Attributional Style Questionnaire to 39 unipolar depressed patients at the beginning and end of cognitive therapy and at one-year follow-up, and we administered it to 12 bipolar patients during a depressed episode. A pessimistic explanatory style for bad events correlated with severity of depression for unipolars at cognitive therapy intake (r = .56, p < .0002), termination (r = .57, p < .0008), and one-year follow-up (r = .64, p < .0005) and among the bipolars (r = .63, p < .03). Explanatory style and depressive symptoms significantly improved by the end of cognitive therapy and remained improved at one-year follow-up. For the unipolars in cognitive therapy, explanatory style change from intake to termination correlated with change in depressive symptoms from intake to termination (r = .65, p < .0001). These results suggest that explanatory style may be one of the mechanisms of change for unipolar depressive patients undergoing cognitive therapy.
Review on Automatic Air Filter Cleaning System

The air filter is an in intake system permanently removes foreign particles such as dust, dirt and soot from air intake thereby maintaining the performance of the engine and protecting it from damage. The previous research on air filter has shown that they are replaced pre-maturely before reaching full capacity of utilization, loading to increasing maintenance costs and to reduced engine protection against abrasive wear. Also the customer plays a very important part because beyond the warranty period, they have a last word, making decision about replacing or not the air filter. So, in this project we are making automatic cleaning of air filter of heavy vehicle without using manual energy it’s completely automatic. If it is done by manually air filter cleaning may be uneven but by this process cleaning should be proper and maintaining the efficiency of air filter as well as engine. The effects of air filter performance were studied carried out with the different diesel engine of cleaned air filter. Index Terms – Air filter, Vehicle, Engines, Automatic Cleaning.
A Comparative Study on Probiotic Organisms Isolated from Different food and Milk Products and Medicinal Capsules

A number of health benefits have been claimed for probiotic bacteria such as Lactobacillus species and Enterococcus species. Beneficial effects of food, milk product and medicinal capsules with live microbes (probiotics) on human health are being increasingly promoted by professionals. These benefits include antimicrobial, anti-mutagenic effects, anti-carcinogenic properties, improvement in lactose metabolism, reduction in serum cholesterol and immune system stimulation. Several studies in recent years have shown the benefits deriving from the ingestion of probiotics. The objective of this study was to screen ten potential probiotic organisms from total of 10 different food samples, milks products, and medicinal capsules were collected. The organisms identified on the basis of phenotypic characters and biochemical properties were Enterococci durans, Lactobacillus rhamnous, Lactobacillus plantarum, Lactobacillus paracasei, and Lactobacillus lactis. It was observed that similar types of probiotic organisms were isolated from food items, milk products and medicinal capsules.
Prevalent Seasoning and Cooking Fats, Arterial Stiffness and Blood Lipid Pattern in a Rural Population Sample: Data from the Brisighella Heart Study

Background: Dietary fats have been variably associated with the risk of cardiovascular disease. The aim of our study was to evaluate the association between everyday mainly used dietary fats in cooking and as seasoning and hemodynamic and lipid parameters. Methods: For this study, we selected from the Brisighella Heart Study cohort subjects who were not treated with antihypertensive drugs and report with certainty their daily mean intake of dietary fats in cooking and as seasoning. Depending on the main source of dietary fat, the involved subjects were classified as prevalent extra-virgin olive oil (EVO) users, prevalent corn oil users, prevalent users of different vegetable oils and prevalent animal fat users, and we compared their characteristics. Results: Everyday consumption of EVO as a main seasoning and cooking fat source was significantly associated to lower body mass index, visceral adiposity index, blood pressure, arterial stiffness, and cholesterolemia, when compared with predominantly animal fat users. Corn oil users also had lower blood pressure, arterial stiffness, and cholesterolemia, when compared with predominantly animal fat users, as well. In particular, in an age and systolic blood pressure adjusted model, the predictors of carotid-femoral pulse wave velocity were the prevalent use of EVO (RR = 0.84, 95% CI 0.67–0.94 vs. other prevalent fat sources), LDL-Cholesterol (RR = 1.12, 95% CI 1.02–1.42), serum uric acid (RR = 1.21, 95% CI 1.09–1.54) and estimated GFR (RR = 0.77, 95% CI 0.59–0.99). Conclusions: According to our findings, the choice of everyday seasoning and cooking fat is associated with a different metabolic and haemodynamic pattern.
Tantrum Tool: Development and Open Pilot Study of Online Parent Training for Irritability and Disruptive Behavior.

Objectives: Parent management training is an effective treatment for disruptive behavior disorders but it is often underutilized in clinical settings. Access to care is limited due to logistical barriers as well as limited service availability. This study examines in an open trial the acceptability, feasibility, and clinical effects of a digital parent management training intervention that includes videoconference coaching, called "Tantrum Tool. " Methods: Fifteen children, ages 3-9 years, participated in an open trial of an 8-week intervention. The primary symptom measure was the Disruptive Behavior Rating Scale (DBRS), and the secondary outcome measure was the Affective Reactivity Index (ARI) completed by the primary caregiver before and after treatment. Results: Treatment retention was high (80%), and parents reported a high level of satisfaction with the program. There was a significant reduction in the mean DBRS score from 13.5 ± 5.5 at baseline to 7.3 ± 3.4 at endpoint, p < 0.001. There was also a significant reduction in the mean ARI irritability score from 7.2 ± 2.6 at baseline to 3.75 ± 2.1 at endpoint, p < 0.01. Conclusions: This open pilot study supports the feasibility and acceptability of a digital parent training program for young children with disruptive behavior. Findings provide preliminary support for a clinically meaningful reduction of both disruptive behavior and irritability. Using the Tantrum Tool to deliver online treatment for children could increase access to first-line treatments for disruptive behavior and irritability in young children. ClinicalTrials.gov: NCT03697837.
ARADEEPOPSIS, an Automated Workflow for Top-View Plant Phenomics using Semantic Segmentation of Leaf States

ARADEEPOPSIS is a robust, open-source, and easy-to-use pipeline to extract plant phenotypic traits and to classify leaf states from large-scale image data. Linking plant phenotype to genotype is a common goal to both plant breeders and geneticists. However, collecting phenotypic data for large numbers of plants remain a bottleneck. Plant phenotyping is mostly image based and therefore requires rapid and robust extraction of phenotypic measurements from image data. However, because segmentation tools usually rely on color information, they are sensitive to background or plant color deviations. We have developed a versatile, fully open-source pipeline to extract phenotypic measurements from plant images in an unsupervised manner. ARADEEPOPSIS (https://github.com/Gregor-Mendel-Institute/aradeepopsis) uses semantic segmentation of top-view images to classify leaf tissue into three categories: healthy, anthocyanin rich, and senescent. This makes it particularly powerful at quantitative phenotyping of different developmental stages, mutants with aberrant leaf color and/or phenotype, and plants growing in stressful conditions. On a panel of 210 natural Arabidopsis (Arabidopsis thaliana) accessions, we were able to not only accurately segment images of phenotypically diverse genotypes but also to identify known loci related to anthocyanin production and early necrosis in genome-wide association analyses. Our pipeline accurately processed images of diverse origin, quality, and background composition, and of a distantly related Brassicaceae. ARADEEPOPSIS is deployable on most operating systems and high-performance computing environments and can be used independently of bioinformatics expertise and resources.
Associations Among Maternal Adiposity, Insulin, and Adipokines in Circulation and Human Milk

Background Insulin, leptin, and adiponectin regulate energy balance and may influence infant growth via their presence in human milk. Maternal body mass index has been associated with human milk insulin, leptin, and adiponectin concentrations, but results are inconsistent. Maternal serum hormone concentrations and fat mass may better characterize human phenotype and be more appropriate predictors of human milk insulin, leptin, and adiponectin. Research aim To examine the associations of human milk insulin, leptin, and adiponectin with their concentrations in maternal circulation and with maternal fat mass. Methods Insulin, leptin, and adiponectin were measured in serum and human milk at 1 month postpartum in 25 women. Total body fat mass and fat-free mass were measured using bioelectrical impedance analysis. Linear regression modeling was used to examine associations of serum hormone concentrations or fat mass with human milk insulin, leptin, and adiponectin after adjusting for covariates. Results Serum insulin (p = .007), leptin (p < .001), and adiponectin (p < .001) were each associated with their respective concentrations in human milk. Fat mass was positively associated with insulin (p = .005) and leptin (p < .001), but not with adiponectin (p = .65), in human milk. Conclusions Human milk insulin, leptin, and adiponectin were positively associated with their concentrations in serum, and human milk insulin and leptin were associated with maternal fat mass. Future research is needed to elucidate the role of human milk hormones in infant energy balance and growth.
Analysis of miRNAs precursors in arabidopsis genome

MicroRNAs precursors(pre-miRNAs) play important roles in forming mature microRNAs(miRNAs). Pre-miRNAs have a stem-loop hairpin in their secondary structure,and the lower folding free energy than other small non-coding RNAs.And it's demonstrated that the minimal folding free energy(MFE) is related to RNA length.So while the MFE is picked out as a criterion,the length must be considered.This paper discusses the length,base pair content and MFE of different RNAs,especially the ratio of the MFE to length(MFEL). The MFEL is the sufficient criterion to distinguish pre-miRNAs from all coding and non-coding RNAs.The MFEL for pre-miRNAs is-45.98 kcal/mol,significantly lower than mRNAs(-23.08 kcal/mol),tRNAs(-22.13 kcal/mol),rRNAs(-16.83 kcal/mol). The findings should facilitate the prediction and identification of new miRNAs.
Differences in Metformin and Thiamine Uptake between Human and Mouse Organic Cation Transporter 1: Structural Determinants and Potential Consequences for Intrahepatic Concentrations

The most commonly used oral antidiabetic drug, metformin, is a substrate of the hepatic uptake transporter OCT1 (gene name SLC22A1). However, OCT1 deficiency leads to more pronounced reductions of metformin concentrations in mouse than in human liver. Similarly, the effects of OCT1 deficiency on the pharmacokinetics of thiamine were reported to differ between human and mouse. Here, we compared the uptake characteristics of metformin and thiamine between human and mouse OCT1 using stably transfected human embryonic kidney 293 cells. The affinity for metformin was 4.9-fold lower in human than in mouse OCT1, resulting in a 6.5-fold lower intrinsic clearance. Therefore, the estimated liver-to-blood partition coefficient is only 3.34 in human compared with 14.4 in mouse and may contribute to higher intrahepatic concentrations in mice. Similarly, the affinity for thiamine was 9.5-fold lower in human than in mouse OCT1. Using human-mouse chimeric OCT1, we showed that simultaneous substitution of transmembrane helices TMH2 and TMH3 resulted in the reversal of affinity for metformin. Using homology modeling, we suggest several explanations, of which a different interaction of Leu155 (human TMH2) compared with Val156 (mouse TMH2) with residues in TMH3 had the strongest experimental support. In conclusion, the contribution of human OCT1 to the cellular uptake of thiamine and especially of metformin may be much lower than that of mouse OCT1. This may lead to an overestimation of the effects of OCT1 on hepatic concentrations in humans when using mouse as a model. In addition, comparative analyses of human and mouse orthologs may help reveal mechanisms of OCT1 transport. SIGNIFICANCE STATEMENT OCT1 is a major hepatic uptake transporter of metformin and thiamine, but this study reports strong differences in the affinity for both compounds between human and mouse OCT1. Consequently, intrahepatic metformin concentrations could be much higher in mice than in humans, impacting metformin actions and representing a strong limitation of using rodent animal models for predictions of OCT1-related pharmacokinetics and efficacy in humans. Furthermore, OCT1 transmembrane helices TMH2 and TMH3 were identified to confer the observed species-specific differences in metformin affinity.
Exploring the Multidimensional Assessment of Interoceptive Awareness in youth aged 7-17 years.

OBJECTIVE This study aimed to adapt the Multidimensional Assessment of Interoceptive Awareness (MAIA) questionnaire for younger respondents. METHOD The language of the MAIA was revised and children aged 7-10 years (n = 212) and adolescents aged 11-17 years (n = 217) completed the questionnaire. RESULTS The original eight-factor model was tested for fit using confirmatory factor analysis. The model had an acceptable fit in the total sample and younger subsample and overall fit in the older subsample was adequate following modification. Internal consistency was good, except for the Noticing, Not-Distracting and Not-Worrying scales. Results also demonstrated a negative linear relationship between the trusting scale and age, suggesting that youths may lose trust in their body as they age. CONCLUSION The adapted MAIA can be used with a younger population and, depending on the research question, individual MAIA scales may be selected. The survey is available at https://osher.ucsf. edu/maia.