Miracle-dz/UniFAR-FTUs huggingface dataset:
Yeast contaminants of micropropagated plant cultures

Leifert, C. , Waites, W.M. , Nicholas, J.R. & Keetley, J.W. 1990. Yeast contaminants of micropropagated plant cultures. Journal of Applied Bacteriology69, 471–476. Of 36 yeast strains isolated from contaminated plant cultures 78% were Candida, 20%Rhodotorula and 2% were Cryptococcus species. Strains of Candida guilliermon-dii represented 45% of all yeasts isolated. Yeasts grew rapidly on plant growth media with a pH of between 2.5 and 6.0 and decreased the medium pH to values of between 2.0 and 3.0. Candida yeasts growing on plant medium for 28 d metabolized about 75% of the sucrose and produced fermentation products such as ethanol and acetic acid. In contrast, Rhodotorula did not produce ethanol or acetic acid and only metabolized about 20% of the sucrose. Possible sources of contamination are discussed.
Isolation of cycloclavine from the culture broth of Aspergillus japonicus Saito

medium which consisted of 3% mannitol, 1% glucose, 1% succinic acid, 0.1% KH2PO4, 0.03% MgSO4à"7H2O, and NH4OH to adjust the pH to 5.6. The mycelia were separated by filtration and extracted with 0.1 n H2SO4. The acid extract was combined with the culture filtrate (ca. 45 liters) and brought to pH 10 by the addition of NH4OH.The mixture was extracted repeatedly with ethylacetate. The extract was concentrated to dryness under reduced pressure, and the residue was dissolved in chloroform. The chloroform solution was shaken with 1% phosphoric acid (pH ca. 2) to transfer alkaloids into the acidic phase, which was transferred back into chloroform upon pH adjustment to 10 with NH4OH. The chloroform solution obtained was concentrated to dryness and the residue was adsorbed on a silica gel column (Wakogel 30 g, 02.5 x 10cm) and eluted with a mixture of chloroformmethanol (95 : 5 v/v). Two alkaloids named AJ-1 and AJ-2 were eluted separately from each other.
The Rainbow Knapsack Revisited: Being a Gay Teacher in a World of Heterosexual Privilege

Peggy McIntosh published “White Privilege: Unpacking the Invisible Knapsack” in 1989. Thirty years later, I revisit McIntosh’s seminal work on White privilege to reframe her discussion to one involving heterosexual privilege, most notably as that privilege affects LGBTQ teachers. I rewrite several of McIntosh’s first-person statements to reflect sexual identity instead of race in order to shed light on the pervasive heteronormativity that has the ability to negatively impact lesbian and gay teachers’ experiences in the workplace.
HISTOCHEMICAL STUDY ON ENDOGENOUS DIAMINOBENZIDINE-POSITIVE GRANULES IN THE GLIA CELL OF RAT BRAIN

Endogenous diaminobenzidine (DAB)-positive granules in the astrocyte of rat brain were demonstrated with the peroxidase histochemical method slightly modified by us. The most prominent accumulation of endogenous DAB-positive granules was present in the astrocytic cytoplasm and astrocytic processes of the lateral and ventral marginal zone of area postrema, olivary nucleus, hypothalamic arcuate nucleus, fimbria hippocampi, subependymal periventricular region, stria medullaris thalami and cerebellar flocculus. Astrocytes containing a moderate amount of DAB-positive granules were demonstrated in the olfactory bulb, neocortex, corpus callosum, basal ganglia and other regions of the rat brain.Histoenzymological characteristics of endogenous DAB-positive granules include presence of a “pseudoperoxidase” (hemoglobin-like peroxidase) and a native organelle in the astrocyte itself. Their activity, however, exhibits a 6.5 to 7.6 optimal pH and is inhibited with KCN, CuSO4 and HIO4-NaBH4. Contrarily, this activity was not inhibited where treated with boiling water.The histochemical fine structural finding showed a presence of distinctive highly electron dense bodies, 0.4 to 1. 6μm in diameter, located in the astrocytes and their processes. Highly electron dense reaction products for DAB were also found and may have a possible relation with the lipid-like dense bodies.
Effect of radiation on chiasma frequency in sunflower (Helianthus annuus).

Chiasma frequencies were determined following seed irradiation of three varieties of H. annuus. Histograms of chiasma frequencies between nuclei, show differences between the controls of the three varieties studied. Differing varietal response to radiation was indicated at high doses with a marked reduction in chiasma frequency with dose in one variety and a tendency in this direction in the two others, as shown by the slopes of the regression lines for Xta on dose. Reduction in the mean chiasma frequency is found to be associated with decline in the mean squares within nuclei. There is a lack of correlation between different bivalents of the nucleus both in the control and irradiated material and this, as in other plants, is found to be associated with the symmetrical nature of the karyotype in this plant.
Seasonal size changes in certain diatoms and their possible significance

Cell sizes of two species of diatoms, Stephanodiscus astraea and Asterionella formosa, were determined in algal populations in four water bodies at various times of the year. Changes in the average cell diameter or length were observed throughout the growing seasons. The possible effects of these cell size changes are discussed in relation to parameters such as rate of sinking, nutrient absorption, temperature, viscosity and turbulence.
Seed ecology of the invasive woody plant African Olive (Olea europaea subsp. cuspidata): Implications for management and restoration

Knowledege of the seed ecology of invasive exotic species, including soil seedbank dynamics, is essential to understanding key factors in successful invasion and in identifying management opportunities. African Olive, Olea europaea L. subsp. cuspidata, is an exotic invasive woody plant in Hawaii, Norfolk Island and eastern Australia, and is now well established in the Cumberland Plain region of western Sydney, Australia. In the present study, the key aspects of the seed ecology of African Olive were determined for populations in western Sydney. Extracted seed germinated at a wide range of temperatures, consistent with tolerance of a wide range of climatic conditions. A seed-burial experiment indicated a slow decrease in viability down to 70.3% during the first year, followed by a rapid decline down to 14.7% in the second year. Probit analysis indicated that under field conditions, seed persistence in the soil was ~29 months (2.4 years). In situ germination was low (3.3%) and did not occur until the mechanical constriction of the endocarp was released through decomposition. The woody seed endocarp was found to be permeable to water, indicating that physical dormancy was not imposed by providing a barrier to water uptake. Within its invasive range, African Olive produces abundant seed. However, the rapid loss of viability of soil-stored seed results in a narrow window of opportunity for germination. The short persistence of seed in the soil may provide an opportunity for managers to achieve control of African Olive once mature plants are removed.
Retransmission Diversity with Channel Selectivity for High Reliable and Low Latency Industrial Wireless Control System

The emerging applications in the 5th generation (5G) network, such as industrial control system and Machine-to-Machine (M2M) communication require high reliability and low latency communication at the same time. The retransmission scheme is one of the practical approaches to increase system reliability when other diversity techniques are avoided due to hardware complexity or signiﬁcant transmission delay. In order to address such problems, in this paper, we propose a fast yet reliable retransmission scheme. The proposed retransmission scheme leverages the frequency diversity through simple channel selectivity. The channel selectivity is carried out by employing low complexity subcarrier allocation for selecting other channels in Orthogonal Frequency Division Multiplexing (OFDM) transmission. The evaluation results show that the proposed retransmission method outperforms the conventional scheme. The proposed method can reduce the number of retransmission by up to 30%, corresponding to a success rate of 0.9999 . In addition, it can be employed in an industrial Wireless Local Area Network (iWLAN) system with a large number of users. The achievable control duration is around 52-63 µ s, an improvement of approximately 36% compared to the conventional system.
Small Vessel Tracking Based on Multi Gateway LORA for Indramayu Traditional Fishery

In this study, we use Lora-based data communication for communication between boats and database management systems for vessel. The maximum distance from the Lora is 10 km, and in order to be able to monitor fishermen along the fisherman’s coast in fishing, it is necessary to have a multi-gateway Lora to increase fishermen’s tracking coverage. The system is built using multi gateway Lora installed on the coast. Devices built with a Lora client are installed on a boat with GPS input, the technique of sending data on the device to the gateway uses a time base with a 1-minute period. To minimize data on the gateway that intersects the range of other gateways, a time flag is made to determine the data at a certain time to determine the trajectory of the boat. The result this research is system-monitoring vessel, which is successfully built and can provide fishing boats along the coast with long-range coverage. Based on experiment, multi gateway can be implemented by creating an identical ID gateway, the Lora client will broadcast to the nearest gateway. The maximum distance obtained is 1.35Km NLOS and to cover 20 Km of beach length is needed 15 gateways.
The Reliability of Quantifying the Pancreatic Ductus in Predicting the Operability of Pancreatic Adenocarcinomas

Amac: Pankreas adenokarsinomlarinin operabilitesini ongormede pankreas kanalinin capini degerlendirmek. Materyal-Metot: Histopatolojik olarak kanitlanmis pankreas bas adenokarsinomu olan ve tanidan once nonspesifik semptomlari nedeniyle multidetektor bilgisayarli tomografi uygulanan 30 hastanin (21 erkek, 9 kadin; ort. yas, 64.2 yil; yas araligi 41-93 yil) klinik ve goruntuleme bulgularini inceledik. Eslik eden sekonder bulgular da analiz edildi. Bulgular: Pankreas basi adenokarsinomu olan 30 hasta degerlendirildi. Bunlardan 13’unun (% 43,3) operabl oldugu (A Grubu) ve 17'sinin (% 56,7) radyolojik ve cerrahi olarak inoperabl oldugu (B Grubu) bulundu. Grup A hastalarinda dilate pankreas kanalinin ortalama capi 5.80 mm ve Grup B hastalarinda 9.15 mm olculdu (p = 0.001). Pankreas kanali capinin bez genisligine orani Grup A'da 0.46 ve Grup B'de 0.62 bulundu (p = 0.001). Koledok dilatasyonu, tumor buyuklugu ve ilk basvuru sikayetleri gibi eslik eden sekonder belirtiler iki grup arasinda anlamli bir farklilik gostermedi. Sonuc: Ana pankreas kanali capi ve/veya kanal capinin bez genisligine orani, pankreas adenokarsinomlarinin operabilitesini ongormede faydali olabilir. Amac: Pankreas adenokarsinomlarinin operabilitesini ongormede pankreas kanalinin capini degerlendirmek. Materyal-Metot: Histopatolojik olarak kanitlanmis pankreas bas adenokarsinomu olan ve tanidan once nonspesifik semptomlari nedeniyle multidetektor bilgisayarli tomografi uygulanan 30 hastanin (21 erkek, 9 kadin; ort. yas, 64.2 yil; yas araligi 41-93 yil) klinik ve goruntuleme bulgularini inceledik. Eslik eden sekonder bulgular da analiz edildi. Bulgular: Pankreas basi adenokarsinomu olan 30 hasta degerlendirildi. Bunlardan 13’unun (% 43,3) operabl oldugu (A Grubu) ve 17'sinin (% 56,7) radyolojik ve cerrahi olarak inoperabl oldugu (B Grubu) bulundu. Grup A hastalarinda dilate pankreas kanalinin ortalama capi 5.80 mm ve Grup B hastalarinda 9.15 mm olculdu (p = 0.001). Pankreas kanali capinin bez genisligine orani Grup A'da 0.46 ve Grup B'de 0.62 bulundu (p = 0.001). Koledok dilatasyonu, tumor buyuklugu ve ilk basvuru sikayetleri gibi eslik eden sekonder belirtiler iki grup arasinda anlamli bir farklilik gostermedi. Sonuc: Ana pankreas kanali capi ve/veya kanal capinin bez genisligine orani, pankreas adenokarsinomlarinin operabilitesini ongormede faydali olabilir.
Multi-domain Knowledge Convergence Trajectory Analysis of Strategic Emerging Industries Based on Citation Network and Text Information

: The analysis of technology convergence process for strategic emerging industries is helpful to deeply understand the generation process and development law of industrial technology, thereby helping master the development trend of the field and promoting the healthy development of the industry. To identify the trajectory and degree of technology convergence of the strategic emerging industries, this study conducts a multi-case study on four fields which present a trend of convergence and attract social attention, namely, high-end equipment manufacturing, new-generation information technology, new medicine
Population dynamics and biting rhythm of the anthropophilic sandfly Lutzomyia cruciata (Diptera: Psychdidae) in Southeast, Mexico

Sandflies attracted by human bait were caught in an endemic focus of localized cutaneous leishmaniasis in the state of Campeche, Mexico. Catches were carried out monthly from February 1994 to January 1995 between 18:00 and 22:00 h. Lutzomyia cruciata was the only species caught. The highest population peak of Lu. cruciata was found in March with lesser peaks in February, December 1994, and January 1995. Maximum biting rate of Lu. cruciata was found between 18:00 and 19:00 h. The host-seeking females of Lu. cruciata were directly related to levels of humidity between 88 and 100%. Low and high temperature had a negative effect upon Lu. cruciata activity. The possible role of Lu. cruciata as vector of leishmaniasis in the state of Campeche, Mexico is discussed.
Characterizing mass–volume–density–porosity relationships in a sandy loam soil amended with compost

Abstract: Although compost is widely used as an organic soil amendment or conditioner, little is known of how it affects the characteristics or interactions among soil constituents. To address this, mixture theory was used to describe the mass–volume–density–porosity attributes and interactions among bulk soil, the mineral constituent, and the organic matter constituent of a sandy loam soil in a no-till corn field that had received one-time additions of yard waste compost at rates of 0 (control), 64, 154, and 380 dry t ha-1. Bulk density (BD, 0–10 cm depth) decreased consistently and near-linearly with increasing soil organic matter (SOM) mass fraction (F OM) for all six growing seasons (2012–2017) after compost addition. Fitting mixture theory expressions to BD vs. F OM data and to soil particle density vs. F OM data for 2013–2017 yielded constant mineral and SOM self-packing densities of D M = 1.673 Mg m-3 and D O = 0.335 Mg m-3, respectively, and constant mineral and SOM particle densities of ρ M = 2.760 Mg m-3 and ρ O = 1.409 Mg m-3, respectively. On a self-packing basis, soil mineral and SOM domain porosities were constants at n M = 0.39 and n O = 0.76, respectively. On a bulk soil volume basis, soil mineral and SOM porosities and volume ratios were linear functions of F OM. The porosity and volume characteristics of the SOM domain differed substantially from those of bulk soil and the mineral domain, and may therefore control the agri-environmental performance of soil, given that organic matter influences soil functioning more than mineral matter.
Polyunsaturated Fatty Acids Combined with Equine Chorionic Gonadotropin to Enhance Reproductive Performance in Aged Rabbit Does

Sixty low-conception rate does aged 18 to 24 months (in four groups) were used to determine the effect of replacement of prostaglandin (PG) F2α (PGF2α) injection by oral administration with sunflower oil (Sun) (rich in omega 6) or linseed oil (Lin) (rich in omega 3) on reproductive and productive performance. Group 1 was injected with 20 U of equine chorionic gonadotropin (eCG), 54 h before artificial insemination (AI) and used as reference group. Group 2 was injected with 20 U of eCG+0.5 mg of PGF2α, 54 h before AI. Group 3 was orally given 3 mL of Sun/doe/day, for seven consecutive days before AI+20 U of eCG, 54 h before AI. Group 4 was treated like Group 3 except that the oil was Lin. Aged does treated with eCG+Sun had elevated blood 17-β estradiol concentration (P≤0.01) accompanied with a decrease of progesterone concentrations compared to the other experimental groups. Contrarily, no significant differences were found between eCG+Lin and eCG+PGF2α treatments on the previous two hormones. Likewise, aged does treated with eCG+Sun and eCG+Lin were statistically similar to those injected with ECG+synthetic PGF2α on blood prostaglandin profile, but still significantly higher than the control group. Treatment with eCG+Sun increased the percentage of fertile does (P≤0.01) and the litter size at birth compared to the other experimental groups. In conclusion, replacement of the PGF2α injection by oral administration of Sun or Lin to aged does improved sexual hormone synthesis and secretion, litter size and bunny body weight at birth.
Current Surgical Treatment of Esophageal Cancer-Distal Adenocarcinoma, Multidisciplinary Approach, Evolving Minimal-Invasive Approach at High-Volume Centers

Even in 2020 esophageal cancer stays a severe disease and means a caesura in the life of the affected. Early carcinoma under strict indications and conditions can be safely resected by interventional endoscopy. In locally advanced esophageal cancer of the mid and lower third radical resection stays the gold-standard. The favored approach is the transthoracic esophagectomy with 2-field lymphadenectomy and reconstruction by gastric pullup. In recent years, progress resulted in a notable decrease in morbidity and mortality of these procedures and in an acceptable life quality despite resection and reconstruction. Additionally, multidisciplinary treatment-especially neoadjuvant chemo- and radiochemotherapy-improved long-term survival. Overall it is clear, that the treatment of esophageal cancer in high volume centers is safer and more effective. Challenges for the future are the further development of minimally invasive procedures by laparoscopic, thoracoscopic, or robot-assisted approaches, which results in minimizing the operative trauma and reducing pulmonary complications. With further progress in decoding the esophageal cancer gene, gene-products, and pathways more specific prevention and more targeted therapy could be applied. Standards of treatment of esophageal cancer differ from country to country. This lies mostly in the ethnic differences like incidence, the unequal distribution of squamous cell carcinoma and adenocarcinoma, and in broad differences in concepts and the capability of the health systems. This review focuses on the current surgical diagnostic and curative treatment of esophageal cancer at the majority of high volume centers in Germany by reviewing the associated literature and imbedded by the author's own clinical and academic experiences.
Age and growth determination in a marine catfish using an otolith check technique

A direct and accurate method of age determination using the relative positions of otolith growth checks is described. In the catfish Guleichthys cuerulescens, otolith length and depth were both linearly related to fish length. Checks appeared to be formed twice per year, at the beginning and at the end of the rainy season (late June to late September). Growth was more rapid in the wet season, when water temperatures were at their height. Calculated mean fork lengths of spring-spawned fish after the first four years of life were respectively 11.5, 19.3, 26.3 and 33.1 cm. Few individuals grew beyond 40 cm, the survival of most of the population being five years or less, but a maximum recorded length of 51.5 cm suggested that catfish may occasionally persist into their seventh or eighth year. The shape of the growth curve indicated that, since a permanent seaward migration of older fish is unlikely, then natural mortality among lagoon-resident G. cuerulescens occurs before a growth asymptote is reached.
A study of biochemical parameters among individuals residing in a fluoride endemic area in India

Fluoride (Fl) exerts an inhibitory effect on many metabolic enzymes of various tissues. A study was carried out among individuals residing in a Fl-affected area in India. The biochemical parameters including serum enzyme activities of alkaline phosphatase (ALKP), glutamic pyruvic transaminase (SGPT), lactate dehydrogenase (LDH), and serum creatinine (CRTN) were estimated in 259 subjects compared to 233 controls. The results showed that among the Fl-exposed population, ALKP and SGPT were significantly increased compared to control. The CRTN levels were also significantly increased compared to the control subjects. LDH levels were not markedly altered. Data suggest that Fl exposure affects renal and hepatic functions in endemic areas of India.
Ascorbic Acid and the Glycosaminoglycans

A new concept of a basic mechanism involved in cell proliferation is presented. It is suggested that cells are normally restrained from proliferating by the highly viscous nature of the intercellular glycosaminoglycans. In order to proliferate, cells must escape from this restraint by depolymerizing the glycosaminoglycans in their immediate environment. This process is accomplished by the release of the enzyme hyaluronidase and is kept in check by physiological hyaluronidase inhibitor. There is some evidence that physiological hyaluronidase inhibitor is an oligoglycosaminoglycan that requires ascorbic acid for its synthesis, and perhaps incorporates residues of ascorbic acid. This hypothesis provides an explanation for the pathogenesis of scurvy. It explains the increased requirement for ascorbic acid that occurs in many cell proliferative diseases, including cancer. It indicates the existence of a basic underlying mechanism in many pathological states and suggests a common pattern of treatment. We conclude that ascorbic acid may have much greater therapeutic value than has been generally assigned to it.