Miracle-dz/UniFAR-FTUs huggingface dataset:
Pulmonary hypertension in patients with idiopathic pulmonary fibrosis.

STUDY OBJECTIVES To determine the impact on survival and clinical correlates of pulmonary hypertension (PH) occurring in patients with idiopathic pulmonary fibrosis (IPF). DESIGN Retrospective study. SETTING Tertiary care, referral medical center. PATIENTS Among 487 consecutive patients with IPF, we identified 136 patients who underwent transthoracic echocardiography within 3 months of their initial evaluation at our institution. Patients with left ventricular dysfunction, valvular heart disease, incomplete follow-up, and those in whom pulmonary artery pressures could not be assessed were excluded; the remaining 88 patients were included in this study. Correlations were performed between echocardiographic measures of PH and clinical variables including survival. MEASUREMENTS AND RESULTS The mean (+/- SD) estimated systolic pulmonary artery pressure (SPAP) for the 88 patients was 48 +/- 16 mm Hg (range, 28 to 116 mm Hg). Among pulmonary function parameters, SPAP correlated best with diffusing capacity of the lung for carbon monoxide (D(LCO)), to which it was inversely related. For survival analysis, patients were stratified into three groups: < or = 35 mm Hg (14 patients), 36 to 50 mm Hg (47 patients), and > 50 mm Hg (27 patients). Using the Kaplan-Meier method, the median survival rates for these three groups were 4.8 years, 4.1 years, and 0.7 years, respectively. Those patients with SPAP > 50 mm Hg had significantly worse survival compared to other subgroups (p = 0.009). CONCLUSION In patients with IPF, PH correlates inversely with D(LCO) and has a significant adverse impact on survival, particularly when SPAP is > 50 mm Hg.
Hemoperfusion with Polymyxin B Immobilized Fibers for Urinary Albumin Excretion in Septic Patients with Trauma

We investigated whether microalbuminuria/urinary creatinine ratio (MACR) is increased in septic patients with trauma and whether polymyxin B immobilized fiber (PMX-F) treatment decreases MACR. Twelve trauma patients without sepsis, 18 trauma patients with sepsis, and 10 healthy controls were included in this study. The 18 trauma patients with sepsis were randomly assigned to one of two groups, PMX-F treatment or conventional treatment. Urinary microalbumin and creatinine were measured before and after treatment. Plasma endotoxin levels were determined by endospecy test. Hemoperfusion with PMX-F was carried out twice, for 2 hours, at a flow rate of 100 ml/min. MACR increased in the 30 trauma patients (5.2 ± 2.2 mg/mmol) in comparison to that in the healthy controls (1.0 ± 0.6 mg/mmol, p < 0.01). In the 18 trauma patients with sepsis, MACR after sepsis (16.6 ± 4.8 mg/mmol) was significantly greater than that before sepsis (5.5 ± 2.3 mg/mmol, p < 0.01). There was a significant correlation between plasma endotoxin levels and MACR in septic trauma patients (p < 0.001). MACR was reduced from 17.0 ± 5.0 mg/mmol to 4.2 ± 1.5 mg/mmol (p < 0.01) with PMX-F, and plasma endotoxin levels were also reduced from 34.5 ± 18.5 pg/ml to 10.8 ± 6.6 pg/ml (p < 0.01). Neither MACR nor plasma endotoxin levels were affected by conventional treatment, however. In summary, trauma patients with sepsis appear to show increased MACR, and PMX-F therapy may be effective for attenuating the increase in MACR.
Uncontrolled head oscillations in people with Parkinson’s disease may reflect an inability to respond to perturbations while walking

Fall injuries in people with Parkinson’s disease (PD) are a major health problem. Increased sway while walking is a risk factor and further understanding of this destabilizing gait change may assist with rehabilitation and help prevent falls. Here, stride-to-stride head oscillations are used to help characterise different aspects of gait impairment in 10 people with PD on medication (67 years, SD 4), 10 healthy age-matched (HAM) participants (66 years, SD 7), and 10 young (30 years, SD 7). A wearable accelerometer was used to analyse head oscillations during five repeat 17 m walks by each participant. People with PD had significantly faster transverse plane head oscillations than the HAM or young groups; both along mediolateral (PD 47.2 cm s−1, HAM 32.5 cm s−1, and young 32.7 cm s−1) and anterioposterior axes (PD 33.3 cm s−1, HAM 24.5 cm s−1, and young 20.6 cm s−1). These differences were uncorrelated with reduced vertical oscillation velocity (PD 15.5 cm s−1, HAM 18.8 cm s−1, and young 20.1 cm s−1) and reduced walking speed (PD 1.2 m s−1, HAM 1.4 m s−1, and young 1.4 m s−1). Increased transverse plane head oscillations in people with PD may reflect motor impairment and the inability to respond sufficiently to perturbations while walking, which appears to be distinct from gait hyperkinesia, reduced vertical oscillations, step length, and walking speed.
Energy cost of treadmill walking.

BACKGROUND The purpose of this study was to determine if energy cost of walking (VO2) could be accurately predicted with the simple models which analyze relationship oxygen uptake-speed of walking. A model to predict energy cost of treadmill walking was published firstly 29 years ago. METHODS Employing the new modification of this model from 1986 to analyze VO2-speed of walking relationship leads to the elaboration of a simple linear model, two-compartment linear model, polynomial model of second order and monoexponential model of the metabolic cost of treadmill walking. To verify and compare these models 87 males, age ranged from 19 to 62 years, were evaluated on a motor driven treadmill. They walked at 0% grade at various velocities ranged from 3 to 12 km.h-1. RESULTS The linear model has in range of intensities 3-12 km.h-1 a form of VO2.kg-1 (ml.kg-1.min-1) = 5.228*v (km.h-1)-11.158, r = 0.812, S(EE) = 4.16 ml.kg-1.min-1. The two-compartment linear model has in range of intensities of 3-7 km.h-1 a form of VO2.kg-1 = 3.207*v(km.h-1)-1.777, r = 0.932, and S(EE) = 1.5. In the range of 7.1-12 km.VO2.kg-1 = 7.120*v-29.168, r = 0.901, S(EE) = 3.78. In the range of intensities from 3 to 12 km.h-1 a polynomial model was found in the form VO2.kg-1 = 4.501-0.108*v + 0.379*v2, r = 0.891, S(EE) = 4.43, and the exponential model had a form VO2.kg-1 = 4.360*exp(0.223*v), r = 0.861, S(EE) = 6.84. All these correlation coefficients were highly significant (p < 0.001 in all cases). CONCLUSIONS It was concluded that when applied to adult population, the models provide reasonable estimate of the actual requirement for treadmill walking provided the subjects in a oxygen uptake steady-state. As other researches for VO2/step we have found U-shaped curves of coefficient energy cost of walking. The minimum was at speed about 4 km.h-1. This finding support the speculation that does exists the "optimal" speed of moving which reflects the minimal energy expenditure during the walking.
Binding of the glycan of the major outer membrane protein of Chlamydia trachomatis to HeLa cells

Recent studies have shown that the major outer membrane protein (MOMP) of Chlamydia trachomatis is glycosylated. The glycan of the MOMP of C. trachomatis serovar L2 was separated from the glycoprotein with N-glycanase, reduced with tritiated NaBH4, and tested for its ability to interact with HeLa cells. The [3H]glycan was shown to attach readily to HeLa cells at 25 or 37 degrees C. This process was slower at 4 degrees C. Competition for possibly similar receptor sites on HeLa cells between the glycan and a sugar, an aminosaccharide, or elementary bodies (EBs) was then studied. D-Galactose, D-mannose, or N-acetylglucosamine was shown to reduce the attachment of the glycan to HeLa cells at concentrations of 0.1 to 0.5 M. Sedoheptulose, D-fructose, or sialic acid did not inhibit the binding of glycan to HeLa cells. The presence of at least 100 native or UV-inactivated EBs per HeLa cell interfered with the glycan's ability to bind to HeLa cells. Heat-inactivated EBs did not compete with the glycan for binding. In the reverse situation, nonradiolabeled glycan prevented the EBs from infecting and forming inclusions in HeLa cells. Incubation of [3H]glycan with rabbit immune serum prepared against antigens of whole EB and the MOMP inhibited attachment. In contrast, incubation of glycan with mouse monoclonal antibodies against the protein portion of the MOMP or the chlamydial lipopolysaccharide did not inhibit attachment. These results suggest that the glycan portion of the MOMP is involved in the attachment process of C. trachomatis organisms to HeLa cells.
Disturbance-Observer-Based Control and Related Methods—An Overview

Disturbance-observer-based control (DOBC) and related methods have been researched and applied in various industrial sectors in the last four decades. This survey, at first time, gives a systematic and comprehensive tutorial and summary on the existing disturbance/uncertainty estimation and attenuation techniques, most notably, DOBC, active disturbance rejection control, disturbance accommodation control, and composite hierarchical antidisturbance control. In all of these methods, disturbance and uncertainty are, in general, lumped together, and an observation mechanism is employed to estimate the total disturbance. This paper first reviews a number of widely used linear and nonlinear disturbance/uncertainty estimation techniques and then discusses and compares various compensation techniques and the procedures of integrating disturbance/uncertainty compensation with a (predesigned) linear/nonlinear controller. It also provides concise tutorials of the main methods in this area with clear descriptions of their features. The application of this group of methods in various industrial sections is reviewed, with emphasis on the commercialization of some algorithms. The survey is ended with the discussion of future directions.
Alveolar ridge preservation using autogenous tooth graft versus beta-tricalcium phosphate alloplast: A randomized, controlled, prospective, clinical pilot study

Background: A randomized, prospective clinical, radiographical, and histological study was conducted to evaluate healing after alveolar ridge preservation technique using two different graft materials, namely, a novel autogenous graft material i. e. , autogenous tooth graft (ATG) and beta-tricalcium phosphate (β-TCP) alloplast. Materials and Methods: Fifteen patients undergoing extraction of at least three teeth were selected. Atraumatic extractions were performed. Of the three extraction sockets, one was grafted with ATG, other with β-TCP, and the third was left ungrafted. Cone-beam computed tomography scans were taken immediately after grafting and 4 months postoperatively to check the changes in alveolar crest height and width at all the sites. Three patients in whom implant placement was done after complete healing; bone samples were harvested using a 3 mm diameter trephine during osteotomy preparation from both the ridge preserved sites and studied histologically. Results: There was a statistically significant difference when the changes in width and height of alveolar crest were compared within all the three groups (P < 0.05). Among three sites, ATG-grafted sites showed the most superior results with a minimal reduction in alveolar crest height and width. Histological analysis also showed the same trend with more new bone formation at ATG-grafted sites as compared to β-TCP-grafted sites. Conclusion: Postextraction, ridge preservation leads to more predictable maintenance of alveolar ridge height and width. ATG as compared to β-TCP provided superior results. Based on this, we conclude that ATG material can serve as a better alternative to conventional bone graft materials.
Altered Expression of PERK Receptor Kinases in Arabidopsis Leads to Changes in Growth and Floral Organ Formation

The proline-rich, extensin-like receptor kinase (PERK) family is characterized by a putative extracellular domain related to cell wall proteins, followed by a transmembrane domain and kinase domain. The original member, PERK1, was isolated from Brassica napus (BnPERK1) and 15 PERK1-related members were subsequently identified in the Arabidopsis thaliana genome. Ectopic expression and antisense suppression studies were performed using the BnPERK1 cDNA under the control of the 35S CaMV constitutive promoter and introduced into Arabidopsis. In the case of antisense suppression, the BnPERK1 cDNA shared sufficient sequence similarity to suppress several members of the At PERK family. In both sets of transgenic Arabidopsis, several heritable changes in growth and development were observed. Antisense BnPERK1 transgenic Arabidopsis showed various growth defects including loss of apical dominance, increased secondary branching, and floral organ defects. In contrast, Arabidopsis plants ectopically expressing BnPERK1 displayed a prolonged lifespan with increased lateral shoot production and seed set. Along with these phenotypic changes, aberrant deposits of callose and cellulose were also observed, suggestive of cell wall changes as a consequence of altered PERK expression.
Fabrication and Characterization of an Epitaxial Graphene Nanoribbon-Based Field-Effect Transistor

The static and dynamic characteristics of top-gated graphene nanoribbon-based field-effect transistors were investigated. Multilayer graphene was synthesized by thermal decomposition of Si-face silicon carbide. The impact of the number of graphene layers on device performance was explored. It was found that, with the reduction of the layer number from ten to five, a significant improvement of direct-current characteristics and high-frequency performance can be observed. A high intrinsic current-gain cutoff frequency of 60 GHz and a maximum oscillation frequency of 28 GHz are reported.
Risk assessment of d-limonene: an example of male rat-specific renal tumorigens.

The naturally occurring food constituent d-limonene has been found to cause tumors at high doses only in the kidney of the male rat in association with the development of hyaline droplet nephropathy. In contrast, neither kidney tumors nor the associated nephropathy have been found in female rats or mice at much higher doses. Adult male rats produce large quantities of a specific low-molecular-weight protein in the liver, which is known as alpha 2U-globulin (alpha 2U-g). With administration of sufficient doses of d-limonene to male rats, this protein has been found to accumulate excessively in the P2 segment cells of renal proximal tubules, resulting in hyaline droplet formation as a manifestation of protein overload. Hyaline droplet accumulation is the first stage in a unique sequence of nephropathic lesions (also known as alpha 2U-g nephropathy), including granular casts in the outer medulla and linear mineralization in the papilla. The mechanism underlying protein accumulation appears to be the reversible binding of chemical to alpha 2U-g with subsequent prolongation of its half-life in the tubule cell. In the case of d-limonene, the minor metabolite d-limonene-1,2-oxide has been shown to be the primary chemical species that binds reversibly to alpha 2U-g, impeding the normal process of lysosomal proteinase degradation of alpha 2U-g. The ensuing nephropathy is associated with a sustained increase in compensatory renal tubule cell proliferation, which provides the putative mechanistic link with renal tumor formation possibly through tumor promotion of spontaneously initiated cells or enhanced spontaneous mutagenesis. This proposed mechanism has been supported by additional information, including negative genotoxicity tests for d-limonene and its oxide metabolites, experimentally verified tumor promotion, and enhanced cell proliferation primarily in P2 segment tubule cells in male F344 rats, but no such effects in the alpha 2U-g-deficient NBR rat. The mechanism of d-limonene tumor development does not appear to be possible in humans since neither the quantity nor the type of protein that binds d-limonene or d-limonene-1,2-oxide is present. The deduction that the renal tumors induced in male rats are not relevant to human carcinogenicity in the hazard evaluation step of risk assessment completes the evaluation of human risk for d-limonene. Consequently, it can be concluded that d-limonene does not pose any carcinogenic or nephrotoxic risk to humans. (ABSTRACT TRUNCATED AT 400 WORDS)
RANK (receptor activator of nuclear factor‐κB) and RANKL expression in multiple myeloma

Summary. The new members of the tumour necrosis factor (TNF) receptor‐ligand family, receptor activator of nuclear factor‐κB ligand (RANKL) and its receptor RANK, play a crucial role in osteoclast differentiation and activation. An increased expression of RANKL and/or RANK may be involved in the excessive bone resorption observed in multiple myeloma (MM). We used immunohistochemistry to study RANK and RANKL expression in bone marrow (BM) biopsies obtained at diagnosis in 15 MM patients, six patients with monoclonal gammopathy of undetermined significance (MGUS) and 10 normal BM biopsies. Plasma cells were not labelled with anti‐RANKL or anti‐RANK antibodies. In all biopsies, RANKL was expressed in endosteal bone surface, around vessels and in cells characterized by cytoplasmic expansions. These last cells did not express CD45 and were vimentin positive, corresponding to bone marrow stromal cells. Numerous stromal cells expressed RANKL in MM and MGUS specimens, with a greater expression in MM than in MGUS. Very few cells were stained with anti‐RANKL in normal BM specimens. With the anti‐RANK antibody, small mononuclear cells in the bone microenvironment were positive and were identified as erythroblast cells. In conclusion, we showed that RANKL was expressed in reticular stromal cells, with a greater intensity in myeloma specimens. These results suggest that RANKL overexpressed by bone marrow stromal cells may contribute to the high rate of bone resorption observed in MM.
Sparse Bayesian Learning for subject independent classification with application to SSVEP-BCI

Sparse Bayesian Learning (SBL) is a widely used framework which helps us to deal with two basic problems of machine learning, to avoid overfitting of the model and to incorporate prior knowledge into it. In this work, multiple linear regression models under the SBL framework are used for the problem of multiclass classification when multiple subjects are available. As a case study, we apply our method to the detection of Steady State Visual Evoked Potentials (SSVEP), a problem that arises frequently into the Brain Computer Interface (BCI) paradigm. The multiclass classification problem is decomposed into multiple regression problems. By solving these regression problems, a discriminant vector is learned for further processing. In addition the adoption of the kernel trick and the special treatment of produced similarity matrix provides us with the ability to use a Leave-One-Subject-Out training procedure resulting in a classification system suitable for subject independent classification. Extensive comparisons are carried out between the proposed algorithm, the SVM classifier and the CCA based methodology. The experimental results demonstrate that the proposed algorithm outperforms the competing approaches, in terms of classification accuracy and Information Transfer Rate (ITR), when the number of utilized EEG channels is small.
The Insulin Receptor Catalyzes the Tyrosine Phosphorylation of Caveolin-1*

Our previous studies revealed that insulin stimulates the tyrosine phosphorylation of caveolin in 3T3L1 adipocytes. To explore the mechanisms involved in this event, we evaluated the association of the insulin receptor with caveolin. The receptor was detected in a Triton-insoluble low density fraction, co-sedimenting with caveolin and flotillin on sucrose density gradients. We also detected the receptor in caveolin-enriched rosette structures by immunohistochemical analysis of plasma membrane sheets from 3T3L1 adipocytes. Insulin stimulated the phosphorylation of caveolin-1 on Tyr14. This effect of the hormone was not blocked by overexpression of mutant forms of the Cbl-associated protein that block the translocation of phospho-Cbl to the caveolin-enriched, lipid raft microdomains. Moreover, this phosphorylation event was also unaffected by inhibitors of the MAPK and phosphatidylinositol 3-kinase pathways. Although previous studies demonstrated that the Src family kinase Fyn was highly enriched in caveolae, an inhibitor of this kinase had no effect on insulin-stimulated caveolin phosphorylation. Interestingly, overexpression of a mutant form of caveolin that failed to interact with the insulin receptor did not undergo phosphorylation. Taken together, these data indicate that the insulin receptor directly catalyzes the tyrosine phosphorylation of caveolin.
Overproduction of l-Cysteine andl-Cystine by Escherichia coli Strains with a Genetically Altered Serine Acetyltransferase

ABSTRACT Organisms that overproduced l-cysteine andl-cystine from glucose were constructed by usingEscherichia coli K-12 strains. cysE genes coding for altered serine acetyltransferase, which was genetically desensitized to feedback inhibition by l-cysteine, were constructed by replacing the methionine residue at position 256 of the serine acetyltransferase protein with 19 other amino acid residues or the termination codon to truncate the carboxy terminus from amino acid residues 256 to 273 through site-directed mutagenesis by using PCR. A cysteine auxotroph, strain JM39, was transformed with plasmids having these altered cysE genes. The serine acetyltransferase activities of most of the transformants, which were selected based on restored cysteine requirements and ampicillin resistance, were less sensitive than the serine acetyltransferase activity of the wild type to feedback inhibition by l-cysteine. At the same time, these transformants produced approximately 200 mg ofl-cysteine plus l-cystine per liter, whereas these amino acids were not detected in the recombinant strain carrying the wild-type serine acetyltransferase gene. However, the production ofl-cysteine and l-cystine by the transformants was very unstable, presumably due to a cysteine-degrading enzyme of the host, such as cysteine desulfhydrase. Therefore, mutants that did not utilize cysteine were derived from host strain JM39 by mutagenesis withN-methyl-N′-nitro-N-nitrosoguanidine. When a newly derived host was transformed with plasmids having the altered cysE genes, we found that the production ofl-cysteine plus l-cystine was markedly increased compared to production in JM39.
Ebunka: do cultural differences matter?

A human factors research program currently underway involves Japanese nuclear power operators. Results from this study suggest that the norm of socially desirable behavior appears to influence operators' job performance and the value of that performance as preceived by experts. In Japan, focus on the correct behavior is viewed as positive. This result represents a sharp contrast with results from similar research programs conducted on power plant operators from the US. Such a collectivistic behavior (i.e., focus on social norms and expected behaviors) is viewed negatively in American culture. A comparison of results from Japan and the US may suggest that cultural differences play a significant role in determining the performance of nuclear power plant operators. It is argued that the most important role of future cross-cultural research in the nuclear industry is to contribute to smoother and more effective technology transfer. <<ETX>>