Miracle-dz/UniFAR-FTUs huggingface dataset:
Loop-mediated isothermal amplification (LAMP): An advanced molecular point-of-care technique for the detection of Leishmania infection

Leishmaniasis, caused by protozoan parasites of the Leishmania genus, represents an important health problem in many regions of the world. Lack of effective point-of-care (POC) diagnostic tests applicable in resources-limited endemic areas is a critical barrier to effective treatment and control of leishmaniasis. The development of the loop-mediated isothermal amplification (LAMP) assay has provided a new tool towards the development of a POC diagnostic test based on the amplification of pathogen DNA. LAMP does not require a thermocycler, is relatively inexpensive, and is simple to perform with high amplification sensitivity and specificity. In this review, we discuss the current technical developments, applications, diagnostic performance, challenges, and future of LAMP for molecular diagnosis and surveillance of Leishmania parasites. Studies employing the LAMP assay to diagnose human leishmaniasis have reported sensitivities of 80% to 100% and specificities of 94% to 100%. These observations suggest that LAMP offers a good molecular POC technique for the diagnosis of leishmaniasis and is also readily applicable to screening at-risk populations and vector sand flies for Leishmania infection in endemic areas.
“I Don’t Feel Like the Odd One”: Utilizing Content Analysis to Compare the Effects of Social Media Use on Well-Being Among Sexual Minority and Nonminority US Young Adults

Purpose: Although there is evidence of associations between social media (SM) use and mental well-being among the general population, these associations among lesbian, gay, and bisexual (LGB) persons are poorly understood. This study compared the influence of SM experiences on mental well-being between LGB and non-LGB persons. Design and Setting: Online cross-sectional survey. Participants: National sample of 2408 US adults aged 18 to 30 years. Method: We asked participants to provide examples of when SM affected their well-being separately in good and bad ways. We coded, summed, and used rate ratios (RRs) to compare responses of LGB and non-LGB individuals. Thematically similar codes were described and grouped into categories. Results: Most responses described positive SM effects. However, of 6 codes that were significantly more frequent among LGB respondents, only social capital (RR = 1.58, 95% confidence interval [CI], 1.17-2.12) described a positive effect. Five codes described negative effects of SM for LGB users: negative emotional contagion (RR = 1.28, 95% CI, 1.04-1.58), comparison with others (RR = 1.28, 95% CI, 1.01-1.62), real-life repercussions (RR = 1.86, 95% CI, 1.18-2.94), envy (RR = 2.49, 95% CI, 1.48-4.19), and need for profile management (RR = 2.32, 95% CI, 1.07-5.03). Conclusion: These findings suggest that, for LGB persons, gaining social capital from SM is valuable for establishing and maintaining connections. Increased negative SM experiences may pose a risk for the mental well-being of LGB individuals.
PERCEPÇÃO DOS EDUCADORES INFANTIS qUANTO AO PROCESSO DE ALTERAÇÃO DO PESO EM PRÉ-ESCOLARES THE PERCEPTION OF EARLY CHILDHOOD EDUCATORS ABOUT WEIGHT GAIN IN PRE-SCHOOLERS PERCEPCIÓN DE LOS EDUCADORES INFANTILES SOBRE EL PROCESO DE ALTERACIÓN DE PESO EN NIÑOS PREESCOLARES Pesquisa

Currently, 42 million children are overweight; this is caused, partially, by unhealthy habits acquired in early childhood. The present study aims to identify and characterize the perceptions and beliefs of early childhood educators about childhood overweight, as well as preschool strategies that address the issue. It is a qualitative descriptive exploratory study conducted through semi-structured interviews with four educators and six parents. The results revealed that teachers have accurate perceptions regarding the factors that contribute to body weight gain and its deleterious consequences. They built a repertoire of strategies in order to encourage healthy eating habits; however, such strategies (positive or not) are not employed on a regular basis. Physical activities are not frequent either. In conclusion, the studied preschool institutions are losing the opportunity to influence children’s health in a more effective way.
T. gondii inveSTING in a latent future

Toxoplasma gondii is an obligate protozoan parasite that naturally infects all mammals, where it alters the host environment to establish chronic infection. Wang and colleagues uncover a new role for the T. gondii protein GRA15 in inducing an anti-parasite response via the interferon stimulator STING. This parasite-driven host defense limits Toxoplasma replication while maintaining host survival, creating an ideal niche for the establishment of latency.
Systemic Sclerosis Dermal Fibroblasts Induce Cutaneous Fibrosis Through Lysyl Oxidase–like 4: New Evidence From Three‐Dimensional Skin‐like Tissues

Systemic sclerosis (SSc) is a clinically heterogeneous disease characterized by increased collagen accumulation and skin stiffness. Our previous work has demonstrated that transforming growth factor β (TGFβ) induces extracellular matrix (ECM) modifications through lysyl oxidase–like 4 (LOXL‐4), a collagen crosslinking enzyme, in bioengineered human skin equivalents (HSEs) and self‐assembled stromal tissues (SAS). We undertook this study to investigate cutaneous fibrosis and the role of LOXL‐4 in SSc pathogenesis using HSEs and SAS.
Longitudinal evaluation of liver stiffness and outcomes in patients with chronic hepatitis C before and after short- and long-term IFN-free antiviral treatment

Abstract Background: New direct-acting antiviral drugs can eradicate hepatitis C virus (HCV) infection in over 90% of patients and can even reduce the risk of complications in advanced fibrosis/cirrhosis. The aims of this study were to evaluate (1) changes in fibrosis during and after antiviral treatment and (2) incidence of hepatocarcinoma and mortality in various fibrosis stages. Methods: This is a longitudinal monocentric prospective study. Blood and instrumental examinations were evaluated at baseline, at the end of therapy, and 1 and 2 years following treatment. Results: Two hundred and ninety-six patients with chronic HCV were evaluated, of whom 115 were experienced, 181 were treatment-naïve, and 2 had previous hepatocellular carcinoma (HCC) and were therefore excluded from the study. At baseline, stiffness values were 13.46 ± 9.97 kPa. Out of the 294 HCV patients enrolled, 100 had lymphoproliferative disorders and were evaluated separately. This group of patients showed stiffness values pertaining to the F0–F2 group (mean stiffness values were 6.07 ± 1.68 kPa). All other patients showed stiffness values pertaining to the F3–F4 group (mean stiffness values were 17.93 ± 10.23). No statistically significant difference was found between stiffness at baseline compared to the end of treatment (EOT), while significant differences were found between the baseline, 1 year (p = .05), and 2 year follow-ups (p < .01). Significant differences were found between baseline and EOT, as well as 1 and 2 years after the end of treatment (p < .001) in the F3–F4 group. Four out of 140 patients with baseline cirrhosis developed HCC during the post-treatment follow-up, 1 of whom died. Conclusions: Non-invasive methods provide important prognostic information, particularly concerning the observed regression of fibrosis and could be extremely useful for monitoring patients with long life expectancies after direct-acting antiviral treatment.
Evaluation of lead toxicity on reproduction using the fertility test in rats.

female rats. Group (1), male and female rats were fed on a diet containing 300 mg lead acetate/kg diet. Group (2), the female rats were fed on a diet containing lead acetate while the male rats fed on normal diet. Group (3), the male rats were fed on a diet containing lead acetate while the female rats fed on normal diet. Group (4), served as control. After three months, the fertility test was made and the offspring were counted then weighted at the end of experiment. Three months later, rats in all groups were weighted then sacrificed and specimens from testes, epididymis, ovaries and fallopian tubes were taken for histopathology. Serum samples were collected for determination of sex hormones. In lead acetate treated groups there were a significant decrease in the total body weight as well as testes and ovaries. The number of offspring was significantly decreased. The mean levels of testosterone, progesterone and estrogen in lead acetate treated group were significantly decreased when compared with control group. Histopathological examination of the testes of male rats treated with lead acetate revealed vascular changes in the form of congestion and edema, testicular degeneration, fibrosis and lymphocytic infiltration in the interstitial tissue. Lesions in female rats treated with lead acetate revealed vascular and degenerative changes in the ovaries and metaplasia of the epithelial lining of the fallopian tubes. It was concluded that lead toxicity induced adverse effects on the fertility and reproduction of rats.
Regulation of Osteogenic Markers at Late Stage of Osteoblast Differentiation in Silicon and Zinc Doped Porous TCP

Calcium phosphates (CaPs) are one of the most widely used synthetic materials for bone grafting applications in the orthopedic industry. Recent trends in synthetic bone graft applications have shifted towards the incorporation of metal trace elements that extend the performance of CaPs to have osteoinductive properties. The objective of this study is to investigate the effects of silicon (Si) and zinc (Zn) dopants in highly porous tricalcium phosphate (TCP) scaffolds on late-stage osteoblast cell differentiation markers. In this study, an oil emulsion method is utilized to fabricate highly porous SiO2 doped β-TCP (Si-TCP) and ZnO doped β-TCP (Zn-TCP) scaffolds through the incorporation of 0.5 wt. % SiO2 and 0.25 wt. % ZnO, respectively, to the β-TCP scaffold. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is utilized to analyze the mRNA expression of osteoprotegerin (OPG), receptor activator of nuclear kappa beta ligand (RANKL), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (Runx2) at the later stage of osteoblast differentiation, day 21 and day 28. Results show that the addition of Si and Zn to the β-TCP structure inhibited the β to α-TCP phase transformation and enhance the density without affecting the dissolution properties. Normal BMP-2 and Runx2 transcriptions are observed in both Si-TCP and Zn-TCP scaffolds at the initial time point, as demonstrated by RT-qPCR. Moreover, the addition of both Si and Zn positively regulate the osteoprotegerin: receptor activator of nuclear factor k-β ligand (OPG:RANKL) ratio at 21-days for Si-TCP and Zn-TCP scaffolds. These results demonstrate the effects of Si and Zn doped porous β-TCP scaffolds on the upregulation of osteoblast marker gene expression including OPG, RANKL, BMP-2, and Runx2, indicating the role of trace elements on the effective regulation of late-stage osteoblast cell differentiation markers.
Neuromuscular blockade management for intraoperative neural monitoring

This article reviews the literature on development of neuromuscular blockade management in thyroid surgery with intraoperative neural monitoring (IONM) in the past decade. Neuromuscular blockade management includes the choice of neuromuscular blocking agents (NMBAs) and reversal of neuromuscular blockade by sugammadex. A series of animal study and clinical trials showed NMBAs effect on IONM in thyroid surgery. We summarized five NMBA regimens for IONM: (a) relaxant‐free regimen, (b) depolarizing NMBA‐succinylcholine, (c) titration of nondepolarizing NMBA, and (d) rocuronium combined with sugammadex. The proper management of neuromuscular blockade during IONM has greatly developed over the past decade. The misuse of NMBAs is associated with false IONM interpretations to surgeons. A detailed understanding of NMBAs and neuromuscular blockade management by sugammadex may optimize IONM quality in patients receiving monitored thyroid surgery.
The CSF-1-receptor inhibitor, JNJ-40346527 (PRV-6527), reduced inflammatory macrophage recruitment to the intestinal mucosa and suppressed murine T cell mediated colitis

Background & aims Originally believed to be primarily a disorder of T-cell signaling, evidence shows that macrophage-lineage cells also contribute to the pathogenesis of Crohn’s disease (CD). Colony stimulating factor-1 (CSF-1) is a key regulator of the macrophage lineage, but its role in CD has not been well established. We examined transcriptional data from CD mucosa for evidence of CSF-1 pathway activation and tested JNJ-40346527 (PRV-6527), a small molecule inhibitor of CSF-1 receptor kinase (CSF-1R), for its ability to inhibit disease indices in murine colitis. Methods A CSF-1 pathway gene set was created from microarray data of human whole blood cultured ex vivo with CSF-1 and compared to a TNFα-induced gene set generated from epithelial-lineage cells. Gene set variation analysis was performed using existing Crohn’s mucosa microarray data comparing patients who either responded or failed to respond to anti-TNFα therapy. Commencing day 14 or day 21, mice with T-cell transfer colitis were treated with vehicle or JNJ-40346527 until study termination (day 42). Endpoints included colon weight/length ratios and histopathology scores, and macrophage and T cells were assessed by immunohistochemistry. Mucosal gene expression was investigated using RNAseq. Results Both the CSF-1 and the TNFα gene sets were enriched in the colonic mucosal transcriptomes of Crohn’s disease and in mouse colitis, and expression of both gene sets was highest in patients who did not respond to anti-TNFα therapy. In these patients neither set was reduced by therapy. In the mouse model, JNJ-40346527 inhibited the increase in colon weight/length ratio by ∼50%, reduced histological disease scores by ∼60%, and reduced F4/80+ mononuclear cell and CD3+ lymphocyte numbers. RNAseq analysis confirmed the CSF-1 gene set was sharply reduced in treated mice, as were gene sets enriched in “M1” inflammatory and “M0” resident macrophages and in activated T cells. Conclusions CSF-1 biology is activated in Crohn’s disease and in murine T cell transfer colitis. Inhibition of CSF-1R by JNJ-40346527 was associated with attenuated clinical disease scores and reduced inflammatory gene expression in mice. These data provide rationale for testing JNJ-40346527 (PRV-6527) in human inflammatory bowel disease.
Draft Genome Sequence of Methanobacterium sp. Strain 34x, Reconstructed from an Enriched Electromethanogenic Biocathode

A draft genome sequence of Methanobacterium sp. strain 34x was reconstructed from the metagenome of an enriched electromethanogenic biocathode operated in a microbial electrosynthesis (MES) reactor. Methanobacterium sp. strain 34x has 68.98% nucleotide-level genomic similarity with the closest related methanogen available with a whole-genome assembly, Methanobacterium lacus strain AL-21. This genome will provide insight into the functional potential of methanogens at the biocathodes of MES systems. ABSTRACT A draft genome sequence of Methanobacterium sp. strain 34x was reconstructed from the metagenome of an enriched electromethanogenic biocathode operated in a microbial electrosynthesis (MES) reactor. Methanobacterium sp. strain 34x has 68.98% nucleotide-level genomic similarity with the closest related methanogen available with a whole-genome assembly, Methanobacterium lacus strain AL-21. This genome will provide insight into the functional potential of methanogens at the biocathodes of MES systems.
Youth Labour Insertion in Portugal: an education perspective

Departamento de Sociologia, Universidade do Porto, E-mail: cparente@letras.up. pt; Departamento de Métodos de Pesquisa Social, Instituto Universitário de Lisboa (ISCTE-IUL), CIES-IUL, E-mail: madalena.ramos@iscte.pt; Instituto de Sociologia, Universidade do Porto. E-mail: vanessa.c. marcos@gmail.com; Departamento de Ciências Sociais, Universidade do Porto. E-mail: sofiaacruz@yahoo.com; Instituto de Sociologia, Universidade do Porto, E-mail: hneto@letras.up. pt.
Toblerone: Surface-Based Partial Volume Estimation

Partial volume effects (PVE) present a source of confound for the analysis of functional imaging data. Correction for PVE requires estimates of the partial volumes (PVs) present in an image. These estimates are conventionally obtained via volumetric segmentation, but such an approach may not be accurate for complex structures such as the cortex. An alternative is to use surface-based segmentation, which is well-established within the literature. Toblerone is a new method for estimating PVs using such surfaces. It uses a purely geometric approach that considers the intersection between a surface and the voxels of an image. In contrast to existing surface-based techniques, Toblerone is not restricted to use with any particular structure or modality. Evaluation in a neuroimaging context has been performed on simulated surfaces, simulated T1-weighted MRI images and finally a Human Connectome Project test-retest dataset. A comparison has been made to two existing surface-based methods; in all analyses Toblerone’s performance either matched or surpassed the comparator methods. Evaluation results also show that compared to an existing volumetric method (FSL FAST), a surface-based approach with Toblerone offers improved robustness to scanner noise and field non-uniformity, and better inter-session repeatability in brain volume. In contrast to volumetric methods, a surface-based approach negates the need to perform resampling which is advantageous at the resolutions typically used for neuroimaging.
Detecting discomfort in infants through facial expressions

Objective: Detecting discomfort status of infants is particularly clinically relevant. Late treatment of discomfort infants can lead to adverse problems such as abnormal brain development, central nervous system damage and changes in responsiveness of the neuroendocrine and immune systems to stress at maturity. In this study, we exploit deep convolutional neural network (CNN) algorithms to address the problem of discomfort detection for infants by analyzing their facial expressions. Approach: A dataset of 55 videos about facial expressions, recorded from 24 infants, is used in our study. Given the limited available data for training, we employ a pre-trained CNN model, which is followed by fine-tuning the networks using a public dataset with labeled facial expressions (the shoulder-pain dataset). The CNNs are further refined with our data of infants. Main results: Using a two-fold cross-validation, we achieve an area under the curve (AUC) value of 0.96, which is substantially higher than the results without any pre-training steps (AUC = 0.77). Our method also achieves better results than the existing method based on handcrafted features. By fusing individual frame results, the AUC is further improved from 0.96 to 0.98. Significance: The proposed system has great potential for continuous discomfort and pain monitoring in clinical practice.
A new delivery system based on apple pomace–pectin gels to encourage the viability of antimicrobial strains

This work was aimed to investigate the concept of the valorization of apple processing by-products to produce a new preservation system based on apple pomace gels to encourage the viability of antimicrobial Lactobacillus strains. A high frequency (850 kHz) low power (1.3 W/cm2) ultrasound-stimulated cavitation was used for the structure modulating of gels under low-temperature (50 ℃) conditions. Medium esterified apple pectin was added to apple pomace to improve its texture properties and stability. The monitoring of the process of gelation was performed by using acoustic technique and method, based on the measurement of the distance (parameter h, mm) traveled by a free-falling module. The obtained data were then compared to gel texture measurements. The results suggest that low power ultrasound leads to a reduced jelly mass stickiness and increased gel hardness, compared to the thermally treated sample. The immobilization of probiotic cells in low pectin apple pomace gels did not sufficiently protect the microorganisms. The higher viability of immobilized Lactobacillus paracasei (54–77%) compared to L. plantarum (43–59%) was recorded after incubation at acidic conditions (pH 2.0). The most suitable system for preserving bacterial cells during storage can be the apple pomace–pectin gel containing up to 53% pectin as a stabilizer retaining 84% of viable cells after one-month storage at 4 ℃. The apple pomace–pectin hydrogels with gelation rate (dh/dt) of 0.03–0.05 mm/s can be used for the preservation of bacterial cells as a suitable functional ingredient for food.
Effects of Seasonal Training Load on Performance and Illness Symptoms in Water Polo.

Brisola, GMP, Claus, GM, Dutra, YM, Malta, ES, de Poli, RAB, Esco, MR, and Zagatto, AM. Effects of seasonal training load on performance and illness symptoms in water polo. J Strength Cond Res XX(X): 000-000, 2019-The purpose of the study was to describe the training load distribution of a young female water polo team in different cycles of the season and verify its subsequent effects on specific fitness measured by the repeated sprints ability (RSA) test, aerobic endurance measured by the lactate minimum test, incidence and severity of upper respiratory tract infection (URTI) symptoms, and muscle damage markers. The training load (i.e., rating of perceived exertion × session duration) of 20 young female water polo players (mean ± SD: age = 15.65 ± 1.3 years; body mass = 60.93 ± 11.0 kg; height = 1.62 ± 0.1 m) was monitored, and the incidence and severity of URTI was assessed during part of the season. In addition, we assessed the lactate minimum speed (LMS), RSA, creatine kinase (CK), and lactate dehydrogenase (LDH) blood concentration during the season. The level of significance set was p < 0.05. The training loads were higher in the specific period (p < 0.01), whereas a high incidence of URTI was observed in the general cycle. The LMS was greater in the general cycle (p < 0.05), whereas total time and best time in the RSA test were greater in the competitive cycle (p < 0.05). The CK and LDH concentrations were significantly lower during the competitive cycle (p < 0.01). The general cycle of a female water polo team is critical regarding URTI and muscle damage, even with smaller training loads than the specific period.
Event free survival at 24 months is a strong surrogate prognostic endpoint of peripheral T cell lymphoma

Event free survival at 24 months (EFS24) has been described as a powerful predictor for outcome in several subtypes of B cell lymphoma. However, it was limitedly described in T cell lymphoma. We explored the implication of EFS24 as a predictor marker for peripheral T cell lymphoma (PTCL). We reviewed 293 systemic PTCL patients at 13 nationwide major university hospitals in Thailand from 2007 to 2014. The median event free survival (EFS) and overall survival (OS) of PTCL patients in our cohort was 16.3 and 27.7 months with corresponding 2‐year EFS and 2‐year OS of 45.8% and 51.9%, respectively. A total of 118 patients achieved EFS24 (no events during the first 24 mo). Patients who achieved EFS24 had better OS than patients who did not (2‐y OS 92% vs 18.8%; HR, 0.1; P < .001). The standardized mortality ratio of patients achieving EFS24 was 18.7 (95% CI, 14.6‐22.8). Multivariable analysis demonstrated performance status, histologic subtype, remission status, and EFS24 achievement as independent predictors for OS. Our study affirmed the value of EFS24 as a powerful prognostic factor for PTCL. Further validation in prospective study setting is warranted.