Miracle-dz/UniFAR-FTUs huggingface dataset:
Asymptomatic post‐transplant lymphoproliferative disorder diagnosed at one year protocol renal allograft biopsy

Post‐transplant lymphoproliferative disorder (PTLD) is a neoplastic complication with a potentially fatal outcome that develops as a consequence of immunosuppression, and is mainly associated with Epstein‐Barr virus (EBV) infection. A 70‐year‐old woman underwent a live unrelated, ABO‐incompatible renal transplant for end‐stage renal disease. One year after transplantation, protocol biopsy revealed pathological changes indicative of the histological subtype of ‘early lesions of PTLD’ according to the World Health Organization classification, while the patient showed no clinical signs or symptoms. The patient was finally diagnosed with EBV‐positive PTLD by in situ hybridization for EBER (EBV‐encoded RNA), and was successfully treated based on the reduction of immunosuppression. Protocol biopsy within the first post‐transplant year is the only diagnostic measure to detect asymptomatic early PTLD, which allows for early intervention and leads to better outcomes.
Bayesian estimation of cognitive decline in patients with alzheimer's disease

Recently, there has been great interest in estimating the decline in cognitive ability in patients with Alzheimer's disease. Measuring decline is not straightforward, since one must consider the choice of scale to measure cognitive ability, possible floor and ceiling effects, between‐patient variability, and the unobserved age of onset. The authors demonstrate how to account for the above features by modeling decline in scores on the Mini‐Mental State Exam in two different data sets. To this end, they use hierarchical Bayesian models with change points, for which posterior distributions are calculated using the Gibbs sampler. They make comparisons between several such models using both prior and posterior Bayes factors, and compare the results from the models suggested by these two model selection criteria.
Cytotoxic effect of Carvacrol on human cervical cancer cells

Carvacrol is a component of numerous aromatic plants which has been evaluated for substantial pharmacological properties. Although the carvacrol induced cytotoxicity has already been reported, but no such study have been made on human cervical cancer, HeLa and SiHa cells. Therefore, in the present study an attempt has been made to investigate the cytotoxic effect of carvacrol on cervical cancer cells. Cytotoxicity induced by carvacrol was determined by different assays like MTT assay and LDH assay. Apoptosis was measured by DNA fragmentation assay. The study clearly showed the dose dependent cytotoxic effect of carvacrol in HeLa and SiHa cells at an IC50 of 50 mg L -1 by both the cytotoxic assays respectively. The dying cells showed characteristics of apoptosis such as, DNA fragmentation. The data in the present study clearly demonstrated cytotoxic effects of carvacrol on human cervical cancer cells. Carvacrol could have a potential therapeutic significance in treating cancer.
Studying the central control of food intake and obesity in rats

The central nervous system regulates energy intake and expenditure through a complex network ofneurotransmitters and neuromodulators. It is of great interest to understand the relevance of these systems tothe physiological control of energy balance and to the disturbances of obesity. The present paper discussessome of the methods to address this field used at the laboratory of Endocrine Physiology of
Wireless Power Transfer Communications for Leg Lengthening/Shortening Procedures

In this paper, a system for leg lengthening or shortening method using wireless power transfer is examined. The first of three modules in the system is presented here. In this module, the transmitter and receiver circuits, and antennas are the focus for optimal wireless power transfer and eventual usability. The primary aim of the transmitter of having a portable, handheld and operating on batteries is fulfilled. A modified Royer circuit is chosen based on its simplicity and low components count. The objective of receiver coil being less than 20 mm in diameter is also satisfied. Simulations and experimental data of the transmitting and receiving coils are in agreement. Finally, a working handheld portable prototype is developed. Although having a maximum efficiency of only 8.4%, the received power of 2.5 W is sufficient to drive a DC motor.
Gene clusters for S fimbrial adhesin (sfa) and F1C fimbriae (foc) of Escherichia coli: comparative aspects of structure and function

Fimbrial adhesins enable bacteria to attach to eucaryotic cells. The genetic determinants for S fimbrial adhesins (sfa) and for F1C ("pseudotype I") fimbriae (foc) were compared. Sfa and F1C represent functionally distinct adhesins in their receptor specificities. Nevertheless, a high degree of homology between both determinants was found on the basis of DNA-DNA hybridizations. Characteristic differences in the restriction maps of the corresponding gene clusters, however, were visible in regions coding for the fimbrial subunits and for the S-specific adhesin. While a plasmid carrying the genetic determinant for F1C fimbriae was able to complement transposon-induced sfa mutants, a plasmid carrying the genetic determinant for a third adhesin type, termed P fimbriae, was unable to do so. Proximal sfa-specific sequences carrying the S fimbrial structural gene were fused to sequences representing the distal part of the foc gene cluster to form a hybrid cluster, and the foc proximal region coding for the structural protein was ligated to sfa distal sequences to form a second hybrid. Both hybrid clones produced intact fimbriae. Anti-F1C monoclonal antibodies (MAbs) only recognized clones which produced F1C fimbriae, and an anti-S adhesin MAb marked clones which expressed the S adhesin. However, one of four other anti-S fimbriae-specific MAbs reacted with both fimbrial structures, S and F1C, indicating a common epitope on both antigens. The results presented here support the view that sfa and foc determinants code for fimbriae that are similar in several aspects, while the P fimbriae are members of a more distantly related group.
F-actin Binding Region of SPIN90 C-terminus Is Essential for Actin Polymerization and Lamellipodia Formation

We recently reported that SPIN90 is able to bind with several proteins involved in regulating actin cytoskeleton networks, including dynamin, WASP, β PIX, and Nck. Based on these findings, we investigated how SPIN90 regulates the actin cytoskeleton and promotes actin assembly. This study demonstrated that aluminium fluoride-induced localization of SPIN90 to lamellipodia requires amino acids 582-722 at the SPIN90 C-terminus, which is also essential for F-actin binding and Arp2/3 complex mediated polymerization of actin into branched actin filaments. Furthermore, after deletion of the F-actin binding region (582-722 SPIN90) failed to localize at the membrane edge and was unable to promote lamellipodia formation, suggesting that the F-actin binding region in the SPIN90 C-terminus is essential for the formation of branched actin networks and regulation of the actin cytoskeleton at the leading edge of cells.
Taming a Monster: Tackling the Emergent Issues Encountered in Mission Critical System Development

Many large IT systems have become too complex to understand. The complexity stems from the ever-increasing number of technical parts as well as from the increasing number of people involved. Still, the systems are designed, developed, tested, and taken into use without paying too much attention to the issues the inherent system complexity introduces. These issues – called emergent because we are not able to foresee them – result in system failures, downtime, and hasty workarounds and fixes. These, in turn, lead to unsatisfying user experience and unexpected costs. This paper is an experience report on some problems encountered and on the solutions applied during a mission critical SaaS system project, especially from the system testing and monitoring standpoint. Main lessons learned, regarding the ability to control and understand the mission critical service behavior, are that masked production data is a must for performance testing, and that the test cases should be automated early and not developed further and enhanced all the time. One should also remember to trust the performance test results, once assured that the environment has been steady.
Platelet-derived Growth Factor-dependent Cellular Transformation Requires Either Phospholipase Cγ or Phosphatidylinositol 3 Kinase*

Although it has been well established that constitutive activation of receptor tyrosine kinases leads to cellular transformation, the signal relay pathways involved have not been systematically investigated. In this study we used a panel of platelet-derived growth factor (PDGF) β receptor mutants (β-PDGFR), which selectively activate various signal relay enzymes to define which signaling pathways are required for PDGF-dependent growth of cells in soft agar. The host cell line for these studies was Ph cells, a 3T3-like cell that expresses normal levels of the β-PDGFR but no PDGF-α receptor (α-PDGFR). Hence, this cell system can be used to study signaling of mutant αPDGFRs or α/β chimeras. We constructed chimeric receptors containing the αPDGFR extracellular domain and the βPDGFR cytoplasmic domain harboring various phosphorylation site mutations. The mutants were expressed in Ph cells, and their ability to drive PDGF-dependent cellular transformation (growth in soft agar) was assayed. Cells infected with an empty expression vector failed to grow in soft agar, whereas introduction of the chimera with a wild-type β-PDGFR cytoplasmic domain gave rise to a large number of colonies. In contrast, the N2F5 chimera, in which the binding sites for phospholipase Cγ (PLC-γ), RasGTPase-activating protein, phosphatidylinositol 3 kinase (PI3K), and SHP-2 were eliminated, failed to trigger proliferation. Restoring the binding sites for RasGTPase-activating protein or SHP-2 did not rescue the PDGF-dependent response. In contrast, receptors capable of associating with either PLC-γ or PI3K relayed a growth signal that was comparable to wild-type receptors in the soft agar growth assay. These findings indicate that the PDGF receptor activates multiple signaling pathways that lead to cellular transformation, and that either PI3K or PLC-γ are key initiators of such signal relay cascades.
Role of Impulsivity and Other Personality Dimensions in Attempted Suicide with Self-Poisoning among Children and Adolescents

The objective of this study was to clarify the role of impulsivity and personality dimensions in attempted suicide among youth. The study included 120 youths who attempted suicide and 100 matched controls. Attempters of suicide had higher total scores and subscales of impulsivity than controls. They had higher novelty seeking, harm avoidance, and reward dependence scores, lower scores on persistence, self-directedness, and cooperativeness. A total of 77.5% of suicide attempters had psychiatric disorders compared to 2% of controls. Main factors predicting the occurrence of suicidal attempts were the presence of psychiatric disorder, impulsivity, stressful life events, and high reward dependence of suicide attempters. The study supports that impulsivity and personality traits play an important role in youth suicide independently or as a part of other interacting factors.
Comparative Study in the Diagnosis of Anemia by Sysmex Kx-21n Hematology Analyzer with Peripheral Blood Smear

In this era, automated peripheral blood count for the diagnosis of anemia is widely accepted in routine practice. Despite the sophistication of present day instruments, there is still need to depend on manual microscopic scan of peripheral blood smear for the diagnosis and appropriate treatment of different types of anemia. Also, the correlation between automated hematology analyzer and manual microscopic scan is rare and conflicting. Hence, the present study was designed to demonstrate a procedure that, the uses of manual scan is to validate rather than to replace automation. The benefit of manual microscopic scan is the ability to identify clinically significant cell type that are not quantifiable by instruments as it “flags” on such type of cells. Therefore, automated hematology analyzer are as reliable as standard manual scan, even though the latter gives additional diagnostic information through blood pictures and less imprecision and technical error over automation. Patient care and lab operations could be optimized as a validation procedure rather than as a reflexive substitute. Hence manual microscopic scan is still gold standard in the diagnosis and classification of anemia and other blood disorders. Whereas usage of automated method would ease our workload and save time for patients.
ACUTE SYSTEMIC COMPLICATIONS IN THE PRETERM FETUS AFTER ASPHYXIA: ROLE OF CARDIOVASCULAR AND BLOOD FLOW RESPONSES

1 Poor perfusion of the kidneys and gut, and associated functional impairment, are major problems in the first days of life in very preterm infants. These complications can be associated with a substantial mortality and further problems such as reduced kidney growth and chronic renal problems in later childhood. 2 There is very little information, and consequently considerable debate, about how or even whether to improve perfusion of the vital organs of this most vulnerable group of babies. Current treatments simply do not consistently improve babies’ perfusion generally or kidney and gut perfusion and function in particular. 3 In this review we critically examine clinical and experimental evidence that suggests that exposure to low oxygen levels before and during birth may be a significant contributor to impaired systemic perfusion, and highlight areas requiring further research. 4 This knowledge is essential to develop and refine ways of improving perfusion of the kidneys and other vital organs in premature babies.
Severe thoracic dystrophy with striking micromelia, abnormal osseous development, including the spine, and multiple visceral anomalies.

Two stillborn female siblings with severe micromelic dwarfism, marked thoracic dystrophy, and gross, generalized osseous dysplasia are described.Multiple internal anomalies including anorectal anomalies, congenital heart disease, and pulmonary hypoplasia were present.This pattern of maldevelopment has not been reported previously. Some of the features were similar to those of the Ellis-van Creveld syndrome and the ATD syndrome described by Jeune et al.Additional clinical and roentgenographic features, however, suggest that these siblings may have manifested a new and distinct malformation syndrome.
A SINGLE NUCLEOTIDE POLYMORPHISM IN THE RAD 51 GENE IN COLORECTAL CANCER

Received: September 27, 2002. *Correspondence. Fax: +48-42 635-44-84; E-mail: januszb@biol.uni. lodz.pl The mammalian RAD51 gene is a homologue of the yeast RAD51 and E. coli RecA genes, which are involved in the genetic recombination the repair of DNA double-strand breaks, and SOS responses to DNA damage. RAD51 gene product (hRAD51, m.w. 37 kD) can bind to singleand double-stranded DNA and exhibit DNA-dependent ATPase activity [13]. OverA SINGLE NUCLEOTIDE POLYMORPHISM IN THE RAD51 GENE IN COLORECTAL CANCER
The coupling between ftsZ transcription and initiation of DNA replication is not mediated by the DnaA‐boxes upstream of ftsZ or by DnaA

The DnaA protein of Escherichia coli is a multifunctional protein which, in addition to promoting initiation of replication, can regulate the initiation or termination of transcription of a variety of genes. It acts by binding to DNA at a defined sequence, termed a DnaA‐box. Three candidate DnaA‐boxes which occur within the essential cell‐division genes, ftsQ and ftsA, have been hypothesized to mediate the response of the downstream ftsZ gene to intracellular levels of DnaA, and thus to couple the processes of initiation and cell division. We show here that, although transcription from promoters upstream of ftsZ is increased when initiation of chromosome replication is blocked by DnaA inactivation, this response is not mediated by the DnaA‐boxes near these promoters, nor is it specific to DnaA. We show, furthermore, that mutational inactivation of the putative DnaA‐binding sites in the fts region of the chromosome does not lead to impaired growth or reduced survival of cells.
Regulation of Chromatin Remodeling by Inositol Polyphosphates

Chromatin remodeling is required for efficient transcription of eukaryotic genes. In a genetic selection for budding yeast mutants that were defective in induction of the phosphate-responsive PHO5 gene, we identified mutations inARG82/IPK2, which encodes a nuclear inositol polyphosphate kinase. In arg82 mutant strains, remodeling ofPHO5 promoter chromatin is impaired, and the adenosine triphosphate–dependent chromatin-remodeling complexes SWI/SNF and INO80 are not efficiently recruited to phosphate-responsive promoters. These results suggest a role for the small molecule inositol polyphosphate in the regulation of chromatin remodeling and transcription.
La bru tueuse

Mythes et rites revelent que dans les societes patrilineaires et virilocales, a l'exemple de celle des Beti, les rapports entre la femme du fils et la mere de l'epoux sont souvent tendus. Tant que la societe beti a connu un equilibre, ce conflit est reste latent et la tradition a su le resoudre. Mais les bouleversements qu'a connu l'Afrique l'ont exacerbe. Pour comprendre l'origine de cette resurgence, il faut se pencher sur la relation œdipienne et etudier certains rites comme l' ekamba , le changement du mode de residence ou de production economique ou encore la disparition de certaines institutions, comme l'initiation so .
The DecisionDx-UM Gene Expression Profile Test Provides Risk Stratification and Individualized Patient Care in Uveal Melanoma

Uveal melanoma (UM) is the most common primary cancer of the eye and has a strong propensity for metastasis. Although there have been many recent improvements in the diagnosis and treatment of UM, and only 2-4% of patients present with detectable metastasis, up to half of patients are at risk for dying of metastatic disease. Clinicopathologic factors are not accurate enough for individualized patient care. Chromosomal alterations have been used for prognostic purposes, but the routine clinical use of these methods is limited by their susceptibility to sampling error resulting from tumor heterogeneity, limited clinical validation, lack of standardized testing platforms, and high technical failure rates. In contrast, the DecisionDx-UM gene expression profile test is a stand-alone platform which requires no other information for maximal prognostic accuracy and which circumvents many of the drawbacks of chromosomal methods through the use of a highly sensitive microfluidics, PCR-based platform that simultaneously measures the expression of 15 carefully selected genes from primary uveal melanoma samples obtained by fine needle biopsy. Low metastatic risk is reported as Class 1, and high metastatic risk as Class 2. The test allows patients to be stratified into risk categories such that high-risk patients can be offered intensive metastatic surveillance and adjuvant therapy while low-risk patients can be spared these interventions. This test is now used as part of the standard of care in many ocular oncology centers.
Sputum eosinophilia after asthmatic responses induced by isocyanates in sensitized subjects

To assess the nature and the time–course of the cellular component of airway inflammation induced by isocyanates, we examined nine subjects with occupational asthma induced by toluene– or methylene diphenyl–diisocyanate (TDI, MDI) and four control subjects never exposed to isocyanates. Sputum was induced by inhalation of ultrasonically nebulized hypertonic saline (3–4% NaCl) before and 8, 24, 48 h after inhalation challenge with TDI or MDI. Expectorated samples were incubated with dithiothreitol, washed and cytocentrifuged. Differential cell counts were obtained on slides stained with May–Grünwald–Giemsa. Metachromatic cells (mast cells and basophils) were counted on slides stained with toluidine blue at pH 0.1. One occupational asthmatic exhibited a dual reaction to TDI, two exhibited a single early asthmatic reaction to M DI, six exhibited a late asthmatic reaction to TDI (n= 5) or M DI (n= 1), whereas no reactions were observed in control subjects after TDI challenge. In sensitized subjects eosinophils increased from a median value (interquartile range) of 5 (15)% before challenge to 29 (29)% (P= 0.014) and to 30 (31)% (P= 0.031) 8 and 24 h after TDI/MDI challenges, respectively. Sputum eosinophilia was observed both in early and late reactors and declined to near to baseline values 48 hr after challenge. Percentages of eosinophils in control subjects did not exceed 7% during the study. There was a significant correlation between the increase in eosinophil counts and the magnitude of the bronchoconstriction expressed as the area of FEV1 response to isocyanate challenge (rank correlation coefficient = 0–71, P= 0.014). No significant changes in sputum neutrophils, macrophages, lymphocytes and mast cells were detected. The results indicate that isocyanate–induced asthmatic responses are associated with influx of eosinophils in airway secretion lasting up to 24 h.
Control of Nonlinear Systems using Neuro-Fuzzy Design tuned with Cooperative Particle Sub-Swarms Optimization

This paper proposes a TSK-type Neuro-Fuzzy system tuned with a novel learning algorithm. The proposed algorithm used an improved version of the standard Particle Swarm Optimization algorithm, it employs several sub-swarms to explore the search space more efficiently. Each particle in a sub-swarm correct her position based on the best other positions, and the useful information is exchanged among the particles during the iteration process. Simulations on Neuro-fuzzy control of two non-linear plants are conduct to verify algorithm performance and indicate that the proposed algorithm outperforms the standard Particle Swarm Optimization algorithm. Keywords— Particle Swarm Optimization; Neuro-Fuzzy design; Cooperative Sub-Swarms; Online Control; Nonlinear Systems
Intelligent Computer-aided Process Planning of Multi-axis CNC Tapping Machine

The increasing complexity of the manufacturing industry demands computer numerical control (CNC) machining, a massively used process that is equipped with an intelligent feature. Not only does its control function revitalize competition in a sustainable manufacturing ecosystem but also advances the Industry 4.0 readiness. This paper develops an intelligent computer-aided process planning (i-CAPP) based on two widely accepted performance measures: manufacturability and efficiency. Machine tapping is a very popular CNC machining process making threaded holes, from which there is an investigation of two manufacturability scenarios. The first scenario deals with all holes with the same size that undergo the same operation (OP). The second one handles the groups of holes with different sizes machined by various OPs. Meanwhile, the study of efficiency focuses on point-to-point drilling, tapping, and chamfering. This research work verifies such indicators by proposing hybrid-two-stage optimization algorithms: 1) traveling salesman problem and 2) innovative Tabu Search. The i-CAPP verification of both indicators was confirmed in an application with a five-axis CNC tapping machine to realize the Industry 4.0 readiness. Extensions of the research in the pursuit of higher intelligent manufacturing platforms are discussed.