Miracle-dz/UniFAR-FTUs huggingface dataset:
What Is the Ideal Blood Pressure Threshold for the Prevention of Atrial Fibrillation in Elderly General Population?

Intensive blood pressure (BP) lowering in patients with hypertension at increased risk of cardiovascular disease has been associated with a lowered risk of incident atrial fibrillation (AF). It is uncertain whether maintaining the optimal BP levels can prevent AF in the general elderly population. We included 115,866 participants without AF in the Korea National Health Insurance Service-Senior (≥60 years) cohort from 2002 to 2013. We compared the influence of BP on the occurrence of new-onset AF between octogenarians (≥80 years) and non-octogenarians (<80 years) subjects. With up to 6.7 ± 1.7 years of follow-up, 4393 incident AF cases occurred. After multivariable adjustment for potentially confounding clinical covariates, the risk of AF in non-octogenarians was significantly higher in subjects with BP levels of <120/<80 and ≥140/90 mm Hg, with hazard ratios of 1.15 (95% confidence interval (CI), 1.03–1.28; p < 0.001) and 1.14 (95% CI, 1.04–1.26; p < 0.001), compared to the optimal BP levels (120–129/<80 mm Hg). In octogenarians, the optimal BP range was 130–139/80–89 mm Hg, higher than in non-octogenarians. A U-shaped relationship for the development of incident AF was evident in non-octogenarians, and BP levels of 120–129/<80 mm Hg were associated the lowest risk of incident AF. Compared to non-octogenarians, the lowest risk of AF was associated with higher BP levels of 130–139/80–89 mm Hg amongst octogenarians.
Periodontal Regeneration using a Xenogeneic Bone Substitute seeded with Autologous Periodontal Ligament derived Mesenchymal Stem Cells: a 12-month quasi-randomized controlled pilot clinical trial.

AIM To evaluate the safety and efficacy of autologous periodontal ligament-derived mesenchymal stem cells (PDL-MSCs) embedded in a xenogeneic bone substitute (XBS) for the regenerative treatment of intra-bony periodontal defects. MATERIAL AND METHODS This quasi-randomized controlled pilot phase II clinical trial included patients requiring a tooth extraction and presence of one intra-bony lesion (1-2 walls). Patients were allocated to either the experimental (XBS + 10x106 PDL-MSCs/100mg) or the control group (XBS). Clinical and radiographical parameters were recorded at baseline, 6, 9 and 12 months. The presence of adverse events was also evaluated. Chi-square, Student`s t-test, U-Mann Whitney, repeated-measures ANOVA and regression models were used. RESULTS Twenty patients were included. No serious adverse events were reported. Patients in the experimental group (n=9) showed greater clinical attachment level (CAL) gain [1.44, standard deviation (SD)=1.87] and probing pocket depth (PPD) reduction (2.33, SD=1.32) than the control group (n=10; CAL gain=0.88, SD=1.68, and PPD reduction=2.10, SD=2.46), without statistically significant differences. CONCLUSION The application of PDL-MSCs to XBS for the treatment of one-two wall intra-bony lesions was safe and resulted in low postoperative morbidity and appropriate healing, although its additional benefit, when compared with the XBS alone, was not demonstrated.
Small but strong: Mutational and functional landscapes of micronuclei in cancer genomes

Micronuclei, small spatially‐separated, nucleus‐like structures, are a common feature of human cancer cells. There are considerable heterogeneities in the sources, structures and genetic activities of micronuclei. Accumulating evidence suggests that micronuclei and main nuclei represent separate entities with respect to DNA replication, DNA damage sensing and repairing capacity because micronuclei are not monitored by the same checkpoints nor covered by the same nuclear envelope as the main nuclei. Thus, micronuclei are spatially restricted “mutation factories. ” Several large‐scale DNA sequencing and bioinformatics studies over the last few years have revealed that most micronuclei display a mutational signature of chromothripsis immediately after their generation and the underlying molecular mechanisms have been dissected extensively. Clonal expansion of the micronucleated cells is context‐dependent and is associated with chromothripsis and several other mutational signatures including extrachromosomal circular DNA, kataegis and chromoanasynthesis. These results suggest what was once thought to be merely a passive indicator of chromosomal instability is now being recognized as a strong mutator phenotype that may drive intratumoral genetic heterogeneity. Herein, we revisit the actionable determinants that contribute to the bursts of mutagenesis in micronuclei and present the growing number of evidence which suggests that micronuclei have distinct short‐ and long‐term mutational and functional effects to cancer genomes. We also pose challenges for studying the long‐term effects of micronucleation in the upcoming years.
Dynamical Behavior of the Human Ferroportin Homologue from Bdellovibrio bacteriovorus: Insight into the Ligand Recognition Mechanism

Members of the major facilitator superfamily of transporters (MFS) play an essential role in many physiological processes such as development, neurotransmission, and signaling. Aberrant functions of MFS proteins are associated with several diseases, including cancer, schizophrenia, epilepsy, amyotrophic lateral sclerosis and Alzheimer’s disease. MFS transporters are also involved in multidrug resistance in bacteria and fungi. The structures of most MFS members, especially those of members with significant physiological relevance, are yet to be solved. The lack of structural and functional information impedes our detailed understanding, and thus the pharmacological targeting, of these transporters. To improve our knowledge on the mechanistic principles governing the function of MSF members, molecular dynamics (MD) simulations were performed on the inward-facing and outward-facing crystal structures of the human ferroportin homologue from the Gram-negative bacterium Bdellovibrio bacteriovorus (BdFpn). Several simulations with an excess of iron ions were also performed to explore the relationship between the protein’s dynamics and the ligand recognition mechanism. The results reinforce the existence of the alternating-access mechanism already described for other MFS members. In addition, the reorganization of salt bridges, some of which are conserved in several MFS members, appears to be a key molecular event facilitating the conformational change of the transporter.
Study on the Anticancer Effect in Vitro of extraction from buckwheat flower and leaf

Objective To observe the assistant effect of EBFL on H22 cells and explore its possible mechanism.Methods MTT method was used to observation on H22 cells in vitro multiplication effect,calculation of the tumor cell growth inhibition rate.Trypan blue staining method method was used to observation the direct cytotoxic effecton on H22 cells in vitro,calculation of average cell rate.Trypan blue staining method method was used to observation the promoting apoptosis on H22 cells in vitro,calculation of cell apoptosis rate.Results EBFL can increase the mouse lymphocyte transformation function,there is a certain dose-response relationship,on H22 cells in vitro cells without the direct cytotoxic effect.It was obvious that EBFL could inhibit tumor cells the proliferation which were cultured in vitro,and EBFL had inhibiting effect.The most obvious was EBFL-M group.CTX and CTX + EBFL-M group could inhibit the proliferation of H22 tumor cells remarkably(P 0.01). Conclusion EBFL can directly inibit trmor cell proliferation and promote its apoptosis.
The therapeutic effect of habit reversal training for Tourette syndrome: a meta-analysis of randomized control trials

ABSTRACT Objectives Comprehensive behavioral intervention for tics (CBIT) and habit reversal training (HRT) are forms of cognitive behavioral therapy that can effectively reduce tic symptoms in patients with tic disorders, but their efficacies and potential moderators were needed to be clarified. Methods In the present study, a meta-analysis was performed to identify the efficacy of HRT and CBIT for individuals with tic disorders. The standard mean difference (SMD) was calculated to assess the effect size of the efficacy of HRT. Subgroup analysis and meta-regression analysis were performed to identify the potential heterogeneity of the SMD of HRT. Results A total of 10 randomized controlled trials (RCTs) including 586 patients with tic disorders were identified. The pooled SMD was −0.43 (95% CI: −0.71, −0.16). The effect size of HRT was moderated by different ‘Comparison Conditions’ (it means the different behavioral therapies in the control group). Conclusion Overall, the authors found a small to medium effect size for the efficacy of HRT. As the most promising behavioral therapy, they conclude that HRT is effective for the treatment of patients with tic disorders. Further high-quality RCTs are needed to determine the efficacy of HRT compared with that of medications.
Analysis of XX SRY-Negative Sex Reversal Dogs

Simple Summary The study of XX SRY-negative sex reversal cases is of great interest because testicular tissue develops in these subjects in the absence of SRY gene, thus allowing us to deepen the knowledge of all the other genes involved in the development of testes and the structures related to the male reproductive apparatus. This paper reports the results of the study of four new canine disorders of sex development (DSDs) XX SRY-negative cases in which 11 copy number variations (CNVs) are observed, five of which are never described. Abstract Impaired fertility associated with disorders of sex development (DSDs) due to genetic causes in dogs are more and more frequently reported. Affected dogs are usually of specific breeds thus representing a cause of economic losses for breeders. The aim of this research is to report the clinical, cytogenetic and molecular genetic findings of four XX SRY-negative DSD dog cases. All the subjects showed a female aspect and the presence of an enlarged clitoris with a penis bone. Morphopathological analyses performed in three of the four cases showed the presence of testes in two cases and ovotestis in another. Conventional and R-banded cytogenetic techniques were applied showing that no chromosome abnormalities were involved in these DSDs. CGH arrays show the presence of 11 copy number variations (CNVs), one of which is a duplication of 458 Kb comprising the genomic region between base 17,503,928 and base 17,962,221 of chromosome 9 (CanFam3 genome assembly). This CNV, confirmed also by qPCR, includes the promoter region of SOX9 gene and could explain the observed phenotype.
Effects of Three Herbs on Methane Emissions from Beef Cattle

Simple Summary Cattle represent a significant source of greenhouse gases (GHGs). In 2010, cattle emitted 5.0 gigatons of CO2 equivalents globally, which represents about 62% of the livestock sector emissions. Therefore, mitigating GHGs such as methane (CH4) originating from the cattle industry, offers an opportunity to reduce GHG emissions and climate change over the short term. Ruminant nutritionists have developed different strategies, which include the use of antibiotics, herbs and chemical compounds, such as nitrate, to manipulate rumen fermentation and reduce CH4 emissions. So, the objectives of the present work were to evaluate the in vivo antimethanogenic effects of three herbs: Cymbopogon citratus (CC), Matricaria chamomilla (MC) and Cosmos bipinnatus (CB) on beef cattle fed a high in concentrate diet and the effects of increasing levels of CC on enteric CH4 emissions by beef cattle fed a ration low in concentrate. We concluded that CC significantly reduced methane yield (g of CH4/kg of DMI) by 33%, CB reduced methane yield by 28%, and MC had no significant effect. In Experiment 2, CC supplemented with 2% of the daily DMI significantly reduced the total daily CH4 emissions by 26% without affecting the supply of nutrients to the animal. Abstract The objectives of the present work were to evaluate the in vivo antimethanogenic effects of Cymbopogon citratus (CC), Matricaria chamomilla (MC) and Cosmos bipinnatus (CB) on beef cattle fed a high in concentrate diet (forage-to-concentrate ratio [F:C] of 19.4:80.6), and the effects of increasing levels of CC (0%, 2%, 3%, and 4% of the daily DM intake (DMI)) on enteric CH4 emissions by beef cattle fed a ration low in concentrate (F:C ratio of 49.3:50.7). Two experiments were conducted to address the objectives. For the first experiment, eight Charolais × Brown Swiss steers distributed in a replicated 4 × 4 Latin square experimental design were used. Four treatments were evaluated: (1) control diet (CO), (2) CO + 365 g dry matter (DM)/d CB, (3) CO + 365 g DM/d MC, (4) CO + 100 g DM/d CC. For Experiment 2, four Charolais x Brown Swiss steers distributed in a single 4 × 4 Latin square design were used. It was concluded that 100 g DM per day CC and 365 g DM per day CB (Experiment 1) reduced CH4 yield of beef cattle. In Experiment 2, CC supplementation levels exceeding 2% of DMI reduced daily CH4 emissions but at the expense of decreasing digestibility of DM.
Cu@Pd/C with Controllable Pd Dispersion as a Highly Efficient Catalyst for Hydrogen Evolution from Ammonia Borane

A series of Cu@Pd/C with different Pd contents was prepared using the galvanic reduction method to disperse Pd on the surface of Cu nanoparticles on Cu/C. The dispersion of Pd was regulated by the Cu(I) on the surface, which was introduced by pulse oxidation. The Cu2O did not react during the galvanic reduction process and restricted the Pd atoms to a specific area. The pulse oxidation method was demonstrated to be an effective process to control the oxidization degree of Cu on Cu/C and then to govern the dispersion of Pd. The catalysts were characterized by transmission electron microscopy (TEM), high-resolution transmission electron microscope (HRTEM), high angular annular dark field scanning TEM (HAADF-STEM), energy-dispersive spectroscopy (EDS) mapping, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), auger electron spectroscopy (AES), and inductively coupled plasma optical emission spectrometer (ICP-OES), which were used to catalyze the hydrogen evolution from ammonia borane. The Cu@Pd/C had much higher activity than the PdCu/C, which was prepared by the impregnation method. The TOF increased as the Cu2O in Cu/C used for the preparation of Cu@Pd/C increased, and the maximum TOF was 465 molH2 min−1 molPd−1 at 298 K on Cu@Pd0.5/C-640 (0.5 wt % of Pd, 640 mL of air was pulsed during the preparation of Cu/C-640). The activity could be maintained in five continuous processes, showing the strong stability of the catalysts.
Peptides with Dual Antimicrobial–Anticancer Activity: Strategies to Overcome Peptide Limitations and Rational Design of Anticancer Peptides

Peptides are naturally produced by all organisms and exhibit a wide range of physiological, immunomodulatory, and wound healing functions. Furthermore, they can provide with protection against microorganisms and tumor cells. Their multifaceted performance, high selectivity, and reduced toxicity have positioned them as effective therapeutic agents, representing a positive economic impact for pharmaceutical companies. Currently, efforts have been made to invest in the development of new peptides with antimicrobial and anticancer properties, but the poor stability of these molecules in physiological environments has triggered a bottleneck. Therefore, some tools, such as nanotechnology and in silico approaches can be applied as alternatives to try to overcome these obstacles. In silico studies provide a priori knowledge that can lead to the development of new anticancer peptides with enhanced biological activity and improved stability. This review focuses on the current status of research in peptides with dual antimicrobial–anticancer activity, including advances in computational biology using in silico analyses as a powerful tool for the study and rational design of these types of peptides.
GRIP1 regulates synaptic plasticity and learning and memory

Significance AMPA receptors (AMPARs) are the principle postsynaptic glutamate receptors mediating fast excitatory synaptic transmission in the brain. Regulation of synaptic AMPAR expression is required for the expression of synaptic plasticity and normal brain function. The turnover of AMPARs within synapses is highly dynamic, and the molecular mechanisms underlying AMPAR trafficking remain unclear. Here we report that GRIP1, an AMPAR-binding protein, plays an essential role in delivering AMPAR into synapses during synaptic plasticity, particularly in long-term potentiation. In addition, the deletion of Grip1 causes synaptic plasticity deficits and impaired learning and memory. Our study reveals a mechanism through which GRIP1 regulates AMPAR trafficking and impacts activity-dependent synaptic strengthening, as well as learning and memory. Hebbian plasticity is a key mechanism for higher brain functions, such as learning and memory. This form of synaptic plasticity primarily involves the regulation of synaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) abundance and properties, whereby AMPARs are inserted into synapses during long-term potentiation (LTP) or removed during long-term depression (LTD). The molecular mechanisms underlying AMPAR trafficking remain elusive, however. Here we show that glutamate receptor interacting protein 1 (GRIP1), an AMPAR-binding protein shown to regulate the trafficking and synaptic targeting of AMPARs, is required for LTP and learning and memory. GRIP1 is recruited into synapses during LTP, and deletion of Grip1 in neurons blocks synaptic AMPAR accumulation induced by glycine-mediated depolarization. In addition, Grip1 knockout mice exhibit impaired hippocampal LTP, as well as deficits in learning and memory. Mechanistically, we find that phosphorylation of serine-880 of the GluA2 AMPAR subunit (GluA2-S880) is decreased while phosphorylation of tyrosine-876 on GluA2 (GluA2-Y876) is elevated during chemically induced LTP. This enhances the strength of the GRIP1–AMPAR association and, subsequently, the insertion of AMPARs into the postsynaptic membrane. Together, these results demonstrate an essential role of GRIP1 in regulating AMPAR trafficking during synaptic plasticity and learning and memory.
Clinical Practice Guidelines for Early Mobilization in the ICU: A Systematic Review

Supplemental Digital Content is available in the text. Objectives: To evaluate the methodological quality and thematic completeness of existing clinical practice guidelines, addressing early mobilization of adults in the ICU. Data Sources: Systematic review of Medline, Embase, CINAHL, Cochrane, and grey literature from January 2008 to February 2020. Study Selection: Two reviewers independently screened titles and abstracts and then full texts for eligibility. Ten publications were included. Data Extraction: A single reviewer extracted data from the included publications and a second reviewer completed cross-checking. Qualitative data were extracted in five categories relating to the key factors influencing delivery of early mobilization to critically ill patients. Data Synthesis: Methodological quality was appraised using the Appraisal of Guidelines for Research and Evaluation II tool. Appraisal of Guidelines for Research and Evaluation II scores for applicability were low. Median quality scores for editorial independence, rigor of development, and stakeholder engagement were also poor. Narrative synthesis of publication content was undertaken. All publications supported implementation of early mobilization. Most documents agreed upon seven topics: 1) early mobilization is safe and may reduce healthcare costs, 2) safety criteria should be provided, 3) a protocolized or structured approach should be used, 4) collaborative teamwork is required, 5) staff require specific skills or experience, 6) patient and family engagement is important, and 7) program evaluation and outcome measurement are a key component of implementation. There was no consensus on dosage and patient selection. The areas of team culture and leadership were poorly addressed. Conclusions: Despite significant variation in the methodological quality of clinical practice guidelines for early mobilization, there were important consistencies in recommendations internationally. Future research should address gaps related to patient selection, dosage, team culture, and expertise. Future clinical practice guidelines in this area should focus on engagement of patients and families in the development process and provision of resources to support implementation based on the consideration of known barriers and facilitators.
In-house rapid colorimetric method for detection of ceftazidime/avibactam resistance in carbapenem resistant Enterobacterales

A rapid colorimetric method, the Andrade screening antimicrobial test, was compared with the E-test method to detect ceftazidime/avibactam (CZA) resistance in carbapenem resistant Enterobacterales clinical isolates. A 106 non-duplicated isolates (86 susceptible and 20 resistant to CZA) were chosen for validation. The sensitivity and specificity were 100%. This method investigates CZA resistance regardless of the resistance mechanism involved. It represents an economical and easy technique that can be applied to routine microbiology laboratories. It allows the detection of CZA resistance at 3 hours of incubation and consequently, the early implementation of accurate therapeutic interventions.
Lower Extremities Strength Differences in Female Volleyball Players With Stage 1 Patellar Tendinopathy

Abstract Kabacinski, J, Murawa, M, Fryzowicz, A, Gorwa, J, and Dworak, LB. Lower extremities strength differences in female volleyball players with stage 1 patellar tendinopathy. J Strength Cond Res 36(8): 2230–2235, 2022—The main purpose of the study was to compare the muscle strength between the involved knee (IK) and uninvolved knee (UK) in 12 female volleyball players with stage 1 chronic patellar tendinopathy. Evaluation included the isometric maximal strength to body mass (MS/BM) of the lower extremities extensors for unilateral and bilateral conditions, concentric isokinetic peak torque to body mass (PT/BM) of the hamstrings and quadriceps at 60°·s−1 and 180°·s−1, IK to UK extensors maximal strength ratio (SR), IK to UK hamstrings (or quadriceps) peak torque ratio (TR), and hamstrings to quadriceps peak TR (H/Q). The results indicated a significantly lower unilateral and bilateral MS/BM of lower extremities extensors for IK than UK (p < 0.001). However, there were no significant differences in SR variable between the unilateral condition and bilateral condition (p = 0.448). Only at the 60°·s−1 velocity, significantly lower quadriceps PT/BM for IK compared with the UK (p < 0.001) and the control group (p < 0.001) were observed. Torque ratio related to the quadriceps group was significantly lower at 60°·s−1 than 180°·s−1 (p = 0.004). Furthermore, significantly higher H/Q for IK compared with UK (p < 0.001) and control group (p = 0.008) only at 60°·s−1 was found. These findings show a significant isometric strength asymmetry for both unilateral and bilateral conditions and a significant concentric strength asymmetry at the low velocity. This study suggests strengthening the weaker quadriceps group and balancing the strength between hamstrings and quadriceps within the knee with patellar tendinopathy.