Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular characterisation of Tyr530Ser and IVS16–1G>T mutations causing severe factor V deficiency

Summary Our previous study reported a missense mutation (Tyr530Ser) and a splicing site mutation (IVS16–1G>T) in blood coagulation factor V (FV) gene in a two-year-old Chinese boy. However, the linkage between the mutations and severe FV deficiency and the underlying mechanism has not been elucidated. The present study was designed to investigate the effect of the two mutations and the possible pathogenetic mechanism. FV procoagulant activity showed tremendous decrease in the patient with two mutations. The bioinformatics analyses predicted that IVS16–1G>T mutation may cause the entire exon 17 of FV to be skipped in transcription and thereby result in a deletion mutant. To confirm the predicted results, the fragment of exon 16 to exon 18 containing IVS16–1G>T mutation was obtained by PCR and site-directed mutagenesis. IVS16–1G>T mutant and wild-type constructs were transfected into COS-7 cells. Sequence analysis showed that mutant transcript lacked the entire 180-bp length of exon 17. Moreover, compared to wild-type, the expression of the two mutant proteins was decreased and the procoagulant activity was also reduced when the deletion mutant cDNA and Tyr530Ser site mutant cDNA were transfected into COS-7 cells, respectively. Our results indicate that Tyr530Ser and IVS16–1G>T could be separately responsible for severe FV deficiency, while the phenotype in the proband could be caused by the combination effect of the two defects.
Research on the Linkage Effect and Market Exploiting of Universities Sports

This article analyzed the linkage effect of universities sports in the society,and has carried on the discussion to its market exploitation.Pointed out the achievement of universities sports in society's linkage effect,can socializes the universities sports,satisfies the demand of society,riches the universities sports culture,alleviates the social stadium which be insufficient,provides project training and the technical guidance for the community inhabitants,advances implementation which the all the people healthy body plans,and the invisible social efficiency,moreover can create the considerable economic efficiency,supple the school's sports funds,renew the stadium hall facility,achieves the "double win" about economic efficiency and the social efficiency.
Reliability Assessment of Solder Joints in Power Electronic Modules by Crack Damage Model for Wind Turbine Applications

Wind turbine reliability is an important issue for wind energy cost minimization, especially by reduction of operation and maintenance costs for critical components and by increasing wind turbine availability. To develop an optimal operation and maintenance plan for critical components, it is necessary to understand the physics of their failure and be able to develop reliability prediction models. Such a model is proposed in this paper for an IGBT power electronic module. IGBTs are critical components in wind turbine converter systems. These are multilayered devices where layers are soldered to each other and they operate at a thermal-power cycling environment. Temperature loadings affect the reliability of soldered joints by developing cracks and fatigue processes that eventually result in failure. Based on Miner’s rule a linear damage model that incorporates a crack development and propagation processes is discussed. A statistical analysis is performed for appropriate model parameter selection. Based on the proposed model, a layout for component life prediction with crack movement is described in details.
Refeeding hypertension in dietary obesity.

A novel model of nutritionally induced hypertension in the rat is described. Dietary obesity was produced by providing sweet milk in addition to regular chow, which elicited a 52% increase in caloric intake. Despite 54% greater body weight gain and 139% heavier retroperitoneal fat pads, 120 days of overfeeding failed to increase systolic pressure in the conscious state (125 +/- 8 vs. 121 +/- 4 mmHg in chow-fed controls) or mean arterial pressure under urethan anesthesia (71 +/- 4 vs. 63 +/- 3 mmHg). In contrast, mild hypertension developed in intermittantly fasted obese animals (a 21-mmHg increase in systolic blood pressure measured in the conscious state and a 16-mmHg increase in mean arterial pressure under anesthesia relative to chow-fed controls). The first 4-day supplemented fast was initiated 4 wk after the introduction of sweet milk, when the animals were 47 g overweight relative to chow-fed controls. Thereafter, 4 days of starvation were alternated with 2 wk of refeeding for a total of 4 cycles. A rapid fall in systolic blood pressure (12 +/- 2 mmHg at 2 days) accompanied the onset of supplemented fasting and was maintained thereafter (2.7 +/- 2.6 mmHg further decrease during the latter half of the fast). With refeeding, blood pressure rose precipitously (13 +/- 3 mmHg in the 1st 2 days), despite poststarvation anorexia. Blood pressure tended to rise slightly over the remainder of the realimentation period (5.2 +/- 2.8 mmHg). After the 4th supplemented fast, hypertension was sustained during 30 days of refeeding. Cumulative caloric intake in starved-refed rats fell within 2% of that in chow-fed controls. (ABSTRACT TRUNCATED AT 250 WORDS)
CS 231 A Project Proposal : Real-Time Airbending Mridul Aanjaneya Stanford

We propose to design a framework for real-time interactive physics-based simulation using state of the art motion sensing devices such as the Microsoft Kinect. The user would be able to interact with the system using simple gestures, where each gesture would have a predefined action associated with it. Unlike previous work on gesture recognition, we would like our recognition to be ‘action aware’ but pose-invariant, meaning that the gesture should be invariant w.r. t view as well as direction. This will require novel pose descriptors which would take into account the intrinsic relationships between different body parts rather than the actual embedding in 3D space. The recognition process will also require the knowledge of a metric on the space of such descriptors for accurate matching. Moreover, the real-time interaction would require fast algorithms for both physical simulation and gesture recognition. We plan on evaluating our method by computing precision and recall for the gesture recognition algorithm and providing an interactive demo.
Persistence of West Nile virus immunoglobulin M antibodies, Greece

A major outbreak of West Nile virus (WNV) lineage 2 infections was observed in 2010 in Greece. In order to check the persistence of WNV IgM antibodies, a second serum sample taken 75–180 days after onset of the illness from 29 patients with WNV infection was tested. A third sample was obtained 181–270 days after onset of the illness from 8 of the 12 patients with IgM‐positive second sample. Mixed effects linear regression analysis indicated that the approximate time at which IgM index became negative was 164 (95% confidence interval, 95% CI 99–236) days after the symptoms' onset. Persistence of IgM antibodies was observed in 12% of patients at 181–270 days of follow‐up. A sharp decrease in the IgM levels was observed, mainly in patients who had high IgM index value in the acute phase. All patients were WNV IgG positive at the follow‐up. J. Med. Virol. 83:1857–1860, 2011. © 2011 Wiley‐Liss, Inc.
Socio-demographic analysis of domestic violence against women: evidences from DHS

i KEYS WORDS. ii DECLARATION. iii DEDICATION. iv ACKNOWLDGMENT . v LIST OF APPENDIX . xviii LIST OF FIGURES . xx LIST OF TABLES . xxiii LIST OF ACRONYMS & ABBREVIATIONS . xxv CHAPTER 1: INTRODUCTION AND SETTING OF THE STUDY . 1 1.
Performance of Random Linear Coding Over Multiple Error-Prone Wireless Links

In this letter we derive an exact formulation for the performance of Random Linear Coding (RLC) when applied over multiple wireless links. We combine this technique with UDP so as to offer a reliable communication service . We extend a previous result, which only considered one single link, to embrace both multiple sources as well as varying quality of wireless links. We establish the number of excess packets that are required to successfully accomplish the communication and, based on the Bianchi model, we calculate the achieved throughput. We also propose a context-aware probabilistic transmission scheme that leads to a relevant performance gain. We use a thorough simulation-based study over the ns-3 framework to assess the validity of the proposed model and to broaden the corresponding analysis.
Multiplexed microbead immunoassays by flow cytometry for molecular profiling: Basic concepts and proteomics applications

Flow cytometry was originally established as an automated method for measuring optical or fluorescence characteristics of cells or particles in suspension. With the enormous increase in development of reliable electronics, lasers, micro-fluidics, as well as many advances in immunology and other fields, flow cytometers have become user-friendlier, less-expensive instruments with an increasing importance for both basic research and clinical applications. Conventional uses of flow cytometry include immunophenotyping of blood cells and the analysis of the cell cycle. Importantly, methods for labeling microbeads with unique combinations of fluorescent spectral signatures have made multiplex analysis of soluble analytes (i.e. the ability to detect multiple targets in a single test sample) feasible by flow cytometry. The result is a rapid, high-throughput, sensitive, and reproducible detection technology for a wide range of biomedical applications requiring detection of proteins (in cells and biofluids) and nucleic acids. Thus, novel methods of flow cytometry are becoming important for diagnostic purposes (e.g. identifying multiple clinical biomarkers for a wide range of diseases) as well as for developing novel therapies (e.g. elucidating drug mechanisms and potential toxicities). In addition, flow cytometry for multiplex analysis, coupled with automated sample handling devices, has the potential to significantly enhance proteomics research, particularly analysis of post-translational modifications of proteins, on a large scale. Inherently, flow cytometry methods are strongly rooted in the laws of the physics of optics, fluidics, and electromagnetism. This review article describes principles and early sources of flow cytometry, provides an introduction to the multiplex microbead technology, and discusses its applications and advantages in comparison to other methods. Anticipated future directions, particularly for translational research in medicine, are also discussed.
Role of Genes That Modulate Host Immune Responses in the Immunogenicity and Pathogenicity of Vaccinia Virus

ABSTRACT Poxvirus vaccine vectors, although capable of eliciting potent immune responses, pose serious health risks in immunosuppressed individuals. We therefore constructed five novel recombinant vaccinia virus vectors which contained overlapping deletions of coding regions for the B5R, B8R, B12R, B13R, B14R, B16R, B18R, and B19R immunomodulatory gene products and assessed them for both immunogenicity and pathogenicity. All five of these novel vectors elicited both cellular and humoral immunity to the inserted HIV-BH10 env comparable to that induced by the parental Wyeth strain vaccinia virus. However, deletion of these immunomodulatory genes did not increase the immunogenicity of these vectors compared with the parental vaccinia virus. Furthermore, four of these vectors were slightly less virulent and one was slightly more virulent than the Wyeth strain virus in neonatal mice. Attenuated poxviruses have potential use as safer alternatives to current replication-competent vaccinia virus. Improved vaccinia virus vectors can be generated by deleting additional genes to achieve a more significant viral attenuation.
Assessment of Additional Endpoints for Trials in Acute Stroke – What, When, Where, in Who?

Functional outcome in acute stroke trials among others is usually measured on the modified Rankin Scale. However, new onset of depression, cognitive decline, and communication deficits alone or in combination affect more than 25% of patients. This report summarizes the findings and conclusions of a workshop by the European Stroke Organization held in February 2011 We propose that assessment of mood disorders, cognitive impairment/dementia, language or communication dysfunction, and quality of life should supplement outcome measures after acute stroke.
Mathematical modeling of marker influx and efflux in cells

The tumor promoter, phorbol 12-myristate 13-acetate (PMA), affects the processing of fluid that enters a cell from the ambient medium. Previous work showed that marker accumulates to a higher level in PMA-treated than in untreated cells. Since PMA also affects the physical activity of the membrane and stimulates the normal process of taking up extracellular fluid, called endocytosis, it is important to learn whether the perturbations in fluid processing can be attributed entirely to a change in the cell’s limiting membrane. To this end, a model for fluid uptake and processing was developed and applied to experiments in which a marker for extracellular fluid was added to cells. From previous work on marker accumulation, it was deduced that there were at least two functional compartments involved in fluid movement. Compartment I is a rapidly filling and rapidly recycling compartment. Compartment II is a slowly filling and emptying compartment. Three routes of vesicle traffic must be considered, one mediating influx from the ambient medium into compartment I, a second, efflux from compartment I to the medium, and a third efflux from compartment I into compartment II. Using earlier models for processing, workers found it difficult to estimate rates of movement through either of the latter routes, as well as the volume of compartment I. The difficulty arises from the fact that only one kinetic constant can be estimated directly from data, namely the instantaneous uptake rate. The remaining data depend on measuring the total mass of marker in the cells. Since the concentration of marker in the cell changes continuously, it is advantageous to employ differential equations to simulate the tracer movement. By applying the model to experimental values, we found estimates for all three rates of fluid movement and the volume of compartment I. It is thought that the model will enable us to determine whether apparent alterations in the time course of uptake arise solely from altered properties of the limiting membrane.
Non-routine activities and the within-city geography of jobs

Externalities are believed to drive the productivity benefits of cities, and also of dense sub-parts within cities, e.g. the central business district (CBD). Recent research claims that density externalities accrue mostly to non-routine activities, and that their effects, e.g. human capital spillovers, attenuate sharply with distance. Consistent with these claims, I demonstrate strong clustering tendencies in non-routine professions as evidenced by job-switching patterns, specifically switchers’ distances moved between employers. Individual-level geo-coded data for switchers within Sweden’s metropolitan areas are used to illustrate that employees hired to non-routine occupations tend to switch to jobs close to the previous work establishment, while blue collar workers show dispersion. The differences are chiefly explained by (1) non-routine activities concentrate in the CBD (the strongest effect) and local employment centres, (2) non-routine activities cluster also outside of centres, and (3) industry-specific effects. The patterns are consistent with the importance of sharply attenuating non-market interactions (e.g. knowledge spillovers) in the production of non-routine products and services.
Evaluation of Dimethoate Toxicity on Pregnancy in Albino Mice

We investigated whether dimethoate, an organophosphorus insecticide, causes abortion or fetal resorption in pregnant albino mice. Graded doses of 16, 20, 24, and 28 mg/kg body weight/d were administered orally from days 7 to day 15 of pregnancy. Laparotomy was performed on day 8 of pregnancy to note the number of implantations, and the animals were autopsied on day 19. The results revealed no inhibition of pregnancy in all dimethoate treated mice relative to a suitable control group. Treatment with 24 or 28 mg dimethoate caused a significant decrease in the number of implantations, live fetuses, and corpora lutea, but a decreased percent fetal survival and increased percent post-implantation loss and gestation length were not significant when compared with control mice. In all mice treated with 28 mg dimethoate, a significant decrease occurred in the body weight of the ovaries, uterus, and liver when compared with control mice. Following treatment with 16 or 20 mg dimethoate, however, no significant change was found in body and organ weights or in the number of implantations, live fetuses, and corpora lutea, percent post-implantation loss, and fetal survival, or gestation length compared with the corresponding parameters in control mice. The results of this study clearly indicate that dimethoate does not cause abortion or fetal resorption in pregnant mice. A significant decrease in the number of corpora lutea and percent fetal survival observed at higher doses of dimethoate could be due to a toxic effect on the embryo or to a hormonal imbalance.
Distribution and Innervation of Taste Buds in the Axolotl

Adult axolotls have approximately 1,400 taste buds in the epithelium of the pharyngeal roof and floor and the medial surfaces of the visceral bars. These receptors are most dense on the lingual surfaces of the upper and lower jaws, slightly less dense throughout lateral portions of the pharyngeal roof and floor, and more sparse within medial portions of the pharyngeal roof and floor, except for a median oval patch of receptors located rostrally between the vomerine tooth fields. Each taste bud is a pear-shaped organ, situated at the center of a raised hillock and averaging 80 and 87 µm in height and width, respectively. Each comprises 50 to 80 cells, which can be classified as basal, dark fusiform, or light fusiform, based on differences in their morphology. The distal ends of the apical processes of the fusiform cells reach the surface of each hillock, forming a single taste pore with an average diameter of 15 µm. Each apical process terminates in one of three ways: as short, evenly spaced microvilli; as long clustered microvilli; or as large, stereocilia-like microvilli. The pharyngeal epithelium and associated taste buds in axolotls are innervated solely by rami of the facial, glossopharyngeal and vagal nerves. Approximately, the rostral one half of the pharyngeal roof is innervated by the palatine rami of the facial nerve, whereas the caudal one half of the pharyngeal roof is innervated by the pharyngeal rami of the glossopharyngeal and vagal nerves. The lingual surface of the lower jaw is innervated by the pretrematic (mandibular) ramus of the facial nerve. The dorsal two-thirds of the visceral arches, and the ventral one-third of the visceral arches and the pharyngeal floor, are innervated by both the pretrematic and post-trematic rami of the glossopharyngeal and vagal nerves, respectively.
The Shadow Economy : An Essay

In this paper the main focus lies on the shadow economy. The most influential fac- tors on the shadow economy are tax policies and state regulation. The size of the shadow economy was decreasing over 1999 to 2007 from 34.0% to 31.2% for 161 countries (un- weighted average).
Oil Revenue, Government Expenditure and Poverty Rate in Nigeria

This study examines the impact of revenue from oil proceeds and disaggregated government spending on poverty rate in Nigeria. Different econometrics tests i.e. pre-estimation test, estimation techniques and diagnostic tests such as Augmented Dickey Fuller, Engel- Granger co-integration, Ordinary Least squares and Granger causality were analysed using the data sets within the period of 1970 and 2013. Empirical result disclosed that gross domestic product and revenue from oil proceeds exert negative effect on poverty rate in Nigeria during the reviewed period. This revealed that oil proceeds being the main revenue source in Nigeria have greater impact in ensuring equal distribution of income as a means of reducing poverty level among her citizens. Painstakingly, these proceeds are not channelled into right directions with regards to government spending on capital projects and recurrent expenditure. This further exacerbates the poverty level in Nigeria.
Synthesis of the psychometric properties of the PTSD checklist (PCL) military, civilian, and specific versions

The posttraumatic stress disorder checklist is a commonly used measure, with military (PCL‐M), civilian (PCL‐C), and specific trauma (PCL‐S) versions. This synthesis of the psychometric properties of all three versions found the PCL to be a well‐validated measure. The PCL shows good temporal stability, internal consistency, test–retest reliability, and convergent validity. The majority of structural validity studies support four factor models. Little is available on discriminant validity and sensitivity to change. Strengths, limitations, and future research directions are discussed. Understanding the PCL's psychometric properties, strengths (e.g., items map on to DSM‐IV diagnostic criteria), and limitations (e.g., may overestimate PTSD prevalence) will help clinicians and researchers make educated decisions regarding the appropriate use of this measure in their particular setting. Depression and Anxiety, 2011. © 2011 Wiley‐Liss, Inc.
The Model of near infrared sensor output voltage as a function of glucose concentration in solution

The number of people with diabetes increases every year in the whole world, including Indonesia. Diabetes is a major cause of stroke, heart attack, kidney failure, lower limb amputation and one of the leading causes of death. In order to manage their blood glucose level, diabetics have to test their blood glucose level as often as possible according to certain medical guidance, diet, exercise and consume medicine regularly. Unfortunately, the current blood glucose testing is inconvenient and uncomfortable, even cause pain for diabetic or patient; therefore, a noninvasive blood glucose measurement is highly desirable. Although several research works have already been done in this area, a successful noninvasive method is still in search. In order to contribute in this research area, we study the effect of glucose concentration in solutions with different concentration to the output voltage of a near infrared sensor as a preliminary research to obtain a successful noninvasive blood glucose meter. In this paper, we reported the model of near infrared sensor output voltage as a function of glucose concentration. The main components of the near infrared sensor are a 1450 nm light emitting diode (LED) as light source, and a photodiode that is sensitive to that wavelength as the sensing device. The distance between LED and photodiode is 15mm. The solutions have 50 mg/dl, 100 mg/dl, 200 mg/dl, 300 mg/dl, and 400 mg/dl glucose concentrations. An acrylic cylinder with 40 mm diameter was filled with 5 ml glucose solution for each concentration. The results show that higher glucose concentrations produce lower sensor output voltages. The linear trend line shows good fit with those data. The value of correlation coefficient is −0.99, which indicates strong relationship between the sensor output voltages and glucose concentrations.