Miracle-dz/UniFAR-FTUs huggingface dataset:
Effects of Deoxynivalenol and Zearalenone on the Histology and Ultrastructure of Pig Liver

The purpose of this study was to determine the effects of single and combined administrations of deoxynivalenol (DON) and zearalenone (ZEN) on the histology and ultrastructure of pig liver. The study was performed on immature gilts, which were divided into four equal groups. Animals in the experimental groups received DON at a dose of 12 μg/kg body weight (BW) per day, ZEN at 40 μg/kg BW per day, or a mixture of DON (12 μg/kg BW per day) and ZEN (40 μg/kg BW). The control group received vehicle. The animals were killed after 1, 3, and 6 weeks of experiment. Treatment with mycotoxins resulted in several changes in liver histology and ultrastructure, including: (1) an increase in the thickness of the perilobular connective tissue and its penetration to the lobules in gilts receiving DON and DON + ZEN; (2) an increase in the total microscopic liver score (histology activity index (HAI)) in pigs receiving DON and DON + ZEN; (3) dilatation of hepatic sinusoids in pigs receiving ZEN, DON and DON + ZEN; (4) temporary changes in glycogen content in all experimental groups; (5) an increase in iron accumulation in the hepatocytes of gilts treated with ZEN and DON + ZEN; (6) changes in endoplasmic reticulum organization in the hepatocytes of pigs receiving toxins; (7) changes in morphology of Browicz–Kupffer cells after treatment with ZEN, DON, and DON + ZEN. The results show that low doses of mycotoxins used in the present study, even when applied for a short period, affected liver morphology.
Mid-Gestation lethality of Atxn2l-Ablated Mice

Depletion of yeast/fly Ataxin-2 rescues TDP-43 overexpression toxicity. In mouse models of Amyotrophic Lateral Sclerosis via TDP-43 overexpression, depletion of its ortholog ATXN2 mitigated motor neuron degeneration and extended lifespan from 25 days to >300 days. There is another ortholog in mammals, named ATXN2L (Ataxin-2-like), which is almost uncharacterized but also functions in RNA surveillance at stress granules. We generated mice with Crispr/Cas9-mediated deletion of Atxn2l exons 5-8, studying homozygotes prenatally and heterozygotes during aging. Our novel findings indicate that ATXN2L absence triggers mid-gestational embryonic lethality, affecting female animals more strongly. Weight and development stages of homozygous mutants were reduced. Placenta phenotypes were not apparent, but brain histology showed lamination defects and apoptosis. Aged heterozygotes showed no locomotor deficits or weight loss over 12 months. Null mutants in vivo displayed compensatory efforts to maximize Atxn2l expression, which were prevented upon nutrient abundance in vitro. Mouse embryonal fibroblast cells revealed more multinucleated giant cells upon ATXN2L deficiency. In addition, in human neural cells, transcript levels of ATXN2L were induced upon starvation and glucose and amino acids exposure, but this induction was partially prevented by serum or low cholesterol administration. Neither ATXN2L depletion triggered dysregulation of ATXN2, nor a converse effect was observed. Overall, this essential role of ATXN2L for embryogenesis raises questions about its role in neurodegenerative diseases and neuroprotective therapies.
Controlled reproduction in Anguilla anguilla (L.): comparison between spontaneous spawning and stripping‐insemination approaches

This study aimed to compare the fertility of eggs between artificially matured female silver eels that spawned spontaneously and those that were spawned by manual stripping. The effects of the two methods of spawning on ovulation and fertilization rate were also investigated. For this purpose, 18 wild female European eels captured in Bonello lagoon (North Adriatic Sea) were carp pituitary extract-injected to undergo sexual maturation and ovulation; a final injection of 17,20β-dihydroxy-4-pregnen-3-one (DHP) was administered when at least 30% of the oocytes were fully transparent. After the DHP-injection, nine eels were transferred to a new closed recirculating aquaculture system, where they were housed with spermiating males (sex ratio 4/1) to allow spontaneous spawning (SPT-group); the remaining nine eels were transferred to a 250 L tank and ovulation was checked at four-hourly intervals by manual stripping (STR-group). The number of eggs per female in the SPT-group was significantly greater than that in the STR-group. Furthermore, fertilization rates in the SPT-group were notably higher than those observed in the STR-group. Significantly, the best performances were obtained among eels in which at least 50% of oocytes were fully transparent at the time DHP was administered. We conclude that the fertility of eggs from spontaneously spawning eels is superior to that of eggs acquired by strip-spawning and artificial fertilization.
Galectin-9 Induced by Dietary Prebiotics Regulates Immunomodulation to Reduce Atopic Dermatitis Symptoms in 1-Chloro- 2,4-Dinitrobenzene (DNCB)-Treated NC/Nga Mice

Atopic dermatitis (AD) is a skin disorder that causes chronic itch. We investigated the inhibitory effects of a mixture of prebiotic short-chain galacto-oligosaccharides and long-chain fructo- oligosaccharides (scGOS/lcFOS), inulin, or β-glucan on AD development in 1-chloro-2,4- dinitrobenzene (DNCB)-treated NC/Nga mice. Mice were randomly assigned to six groups: untreated mice, AD control, positive control (DNCB-treated NC/Nga mice fed a dietary supplement of Zyrtec), and DNCB-treated NC/Nga mice fed a dietary supplement of prebiotics such as scGOS/lcFOS (T1), inulin (T2), or β-glucan (T3). The prebiotic treatment groups (T1, T2, and T3) showed suppression of AD symptoms, Th2 cell differentiation, and AD-like skin lesions induced by DNCB. In addition, prebiotic treatment also reduced the number of microorganisms such as Firmicutes, which is associated with AD symptoms, and increased the levels of Bacteroidetes and Ruminococcaceae, which are associated with alleviation of AD symptoms. Our findings demonstrate the inhibitory effects of prebiotics on AD development by improving the Th1/Th2 cytokine balance and beneficial symbiotic microorganisms in in vitro and in vivo models.
7‐Amidocoumarins as Multitarget Agents against Neurodegenerative Diseases: Substitution Pattern Modulation

This study explores the potential of 7‐amidocoumarins as multitarget agents against Parkinson's and Alzheimer's diseases, by modulating the substitution patterns within the scaffold. Sixteen compounds were synthesized via 7‐amino‐4‐methylcoumarin acylation, and in vitro evaluation of the molecules against hMAO‐A, hMAO‐B, hAChE, hBuChE and hBACE1 was performed. Five compounds turned out to be potent and selective hMAO‐B inhibitors in the nanomolar range, six displayed inhibitory activity of hMAO‐A in the low micromolar range, one showed hAChE inhibitory activity and another one hBACE1 inhibitory activity. MAO‐B reversibility profile of 7‐(4’‐chlorobenzamido)‐4‐methylcoumarin (10) was investigated, with this compound being a reversible inhibitor. Neurotoxicity on motor cortex neurons and neuroprotection against H2O2 were also studied, corroborating the safety profile of these molecules. Finally, theoretical ADME properties were also calculated, showing these molecules as good candidates for the optimization of a lead compound. Results suggest that by modulating the substitution pattern at position 7 of the scaffold, selective or multitarget molecules can be achieved.
Synergy among Exocyst and SNARE Interactions Identifies a Functional Hierarchy in Secretion during Vegetative Growth[CC-BY]

Direct interactions between exocyst and SNARE subunits hierarchically coordinate secretion at the plasma membrane for plant growth. Vesicle exocytosis underpins signaling and development in plants and is vital for cell expansion. Vesicle tethering and fusion are thought to occur sequentially, with tethering mediated by the exocyst and fusion driven by assembly of soluble NSF attachment protein receptor (SNARE) proteins from the vesicle membrane (R-SNAREs or vesicle-associated membrane proteins [VAMPs]) and the target membrane (Q-SNAREs). Interactions between exocyst and SNARE protein complexes are known, but their functional consequences remain largely unexplored. We now identify a hierarchy of interactions leading to secretion in Arabidopsis (Arabidopsis thaliana). Mating-based split-ubiquitin screens and in vivo Förster resonance energy transfer analyses showed that exocyst EXO70 subunits bind preferentially to cognate plasma membrane SNAREs, notably SYP121 and VAMP721. The exo70A1 mutant affected SNARE distribution and suppressed vesicle traffic similarly to the dominant-negative truncated protein SYP121ΔC, which blocks secretion at the plasma membrane. These phenotypes are consistent with the epistasis of exo70A1 in the exo70A1 syp121 double mutant, which shows decreased growth similar to exo70A1 single mutants. However, the exo70A1 vamp721 mutant showed a strong, synergy, suppressing growth and cell expansion beyond the phenotypic sum of the two single mutants. These data are best explained by a hierarchy of SNARE recruitment to the exocyst at the plasma membrane, dominated by the R-SNARE and plausibly with the VAMP721 longin domain as a nexus for binding.
Clinical Evidence and Mechanisms of High-Protein Diet-Induced Weight Loss

Several clinical trials have found that consuming more protein than the recommended dietary allowance not only reduces body weight (BW), but also enhances body composition by decreasing fat mass while preserving fat-free mass (FFM) in both low-calorie and standard-calorie diets. Fairly long-term clinical trials of 6–12 months reported that a high-protein diet (HPD) provides weight-loss effects and can prevent weight regain after weight loss. HPD has not been reported to have adverse effects on health in terms of bone density or renal function in healthy adults. Among gut-derived hormones, glucagon-like peptide-1, cholecystokinin, and peptide tyrosine-tyrosine reduce appetite, while ghrelin enhances appetite. HPD increases these anorexigenic hormone levels while decreasing orexigenic hormone levels, resulting in increased satiety signaling and, eventually, reduced food intake. Additionally, elevated diet-induced thermogenesis (DIT), increased blood amino acid concentration, increased hepatic gluconeogenesis, and increased ketogenesis caused by higher dietary protein contribute to increased satiety. The mechanism by which HPD increases energy expenditure involves two aspects: first, proteins have a markedly higher DIT than carbohydrates and fats. Second, protein intake prevents a decrease in FFM, which helps maintain resting energy expenditure despite weight loss. In conclusion, HPD is an effective and safe tool for weight reduction that can prevent obesity and obesity-related diseases. However, long-term clinical trials spanning more than 12 months should be conducted to further substantiate HPD effects.
The neural networks underlying reappraisal of empathy for pain

Abstract Emotion regulation plays a central role in empathy. Only by successfully regulating our own emotions can we reliably use them in order to interpret the content and valence of others’ emotions correctly. In an functional magnetic resonance imaging (fMRI)-based experiment, we show that regulating one’s emotion via reappraisal modulated biased emotional intensity ratings following an empathy for pain manipulation. Task-based analysis revealed increased activity in the right inferior frontal gyrus (IFG) when painful emotions were regulated using reappraisal, whereas empathic feelings that were not regulated resulted in increased activity bilaterally in the precuneus, supramarginal gyrus and middle frontal gyrus (MFG), as well as the right parahippocampal gyrus. Functional connectivity analysis indicated that the right IFG plays a role in the regulation of empathy for pain, through its connections with regions in the empathy for pain network. Furthermore, these connections were further modulated as a function of the type of regulation used: in sum, our results suggest that accurate empathic judgment (i.e. empathy that is unbiased) relies on a complex interaction between neural regions involved in emotion regulation and regions associated with empathy for pain. Thus, demonstrating the importance of emotion regulation in the formulation of complex social systems and sheds light on the intricate network implicated in this complex process.
The effectiveness of a commercial microbial product in poorly prepared tiger shrimp, Penaeus monodon (Fabricius), ponds

The efficacy of a commercial microbial product was tested in commercial tiger shrimp, Penaeus monodon (Fabricius), ponds for one culture period in Kuala Selangor, Malaysia. Four ponds with replicates for treatment and control were used. The pond bottom was dried but the organic sludge was not removed as normally practised in pond preparation. The ponds were stocked with 15 post-larvae at the rate of 31 m-2. Physical, chemical and biological parameters of the pond were analysed every 2 weeks during the culture period. Water quality parameters remained within the optimum range for shrimp culture except for ammonia-nitrogen being significantly higher in control ponds and silica in treated ponds. Benthic organisms were not found in any of the ponds. The average counts of different bacteria were not significantly higher in treated ponds than control. Because of poor health, the shrimp were harvested earlier (72 days) than the usual 120 days. An average of 875.60 ± 67.00 kg shrimp ha-1 was obtained in treated ponds with a feed conversion ratio (FCR) of 1.57 ± 0.10 and survival rate of 42.35 ± 5.37% compared with 719.50 ± 130.94 kg shrimp ha-1, 2.99 ± 0.70 and 21.25 ± 3.26%, respectively, in control ponds. Neither the microbial product nor the frequent water exchange was effective in overcoming the problems caused by the poor pond bottom.
Familial language network vulnerability in primary progressive aphasia

Objective To investigate evidence of the potential role of early cortical vulnerability in the development of primary progressive aphasia (PPA). Method A woman with a diagnosis of PPA and her 9 adult siblings, 7 with developmental language disabilities, underwent neuropsychological testing, structural MRI, and resting-state fMRI. Whole-exome sequencing was conducted for genes associated with dyslexia or with neurodegenerative dementia. Results The siblings demonstrated lower verbal than nonverbal cognitive test scores in a developmental dyslexia pattern. On structural MRI, although the siblings did not differ from controls in total brain volume, the left hemisphere language area volume was significantly smaller than the right. Furthermore, cortical connectivity between the left superior temporal area, previously identified as the region of peak atrophy in the proband early in the course of illness, and adjacent language network components, including the planum temporale, was decreased in the siblings. No distinctive genetic signatures were identified. Conclusion This report further supports the hypothesis that at least some cases of PPA may be based on a familial language network vulnerability that interferes with the acquisition of language in some members and that makes the language network a locus of least resistance to the effects of an independently late-arising neurodegenerative disease in others. This association offers a conceptual model to explain why identical neurodegenerative diseases may selectively target the language network in some individuals while targeting networks that regulate memory or behavior in others. The genetic basis for this vulnerability remains to be determined.
Moderators of intergroup evaluation in disadvantaged groups: A comprehensive test of predictions from system justification theory.

We examined hypotheses proposed by System Justification Theory (SJT; Jost, Banaji, & Nosek, 2004) regarding intergroup evaluation in disadvantaged groups, using large samples of online participants (total N = 715,721), spanning 8 intergroup domains and 14 nations. Using a meta-analytic approach, we tested these hypotheses at the individual level (as SJT is generally articulated), as well as at the social group level. Consistent with SJT, individual-level analyses revealed that disadvantaged groups demonstrated outgroup favoritism on Implicit Association Tests (IATs; i.e., implicit measures), but demonstrated ingroup favoritism or no intergroup preference on self-report (i.e., explicit) measures. Additionally, these average effects were characterized by high heterogeneity, and follow-up exploratory analyses revealed that intergroup evaluation in disadvantaged groups was moderated by the intergroup domain: Whereas some disadvantaged groups consistently displayed outgroup favoritism (e.g., age, weight), others consistently displayed ingroup favoritism (e.g., sexual orientation, religion), and yet others displayed diverging patterns on implicit and explicit measures (e.g., race, ethnicity). Consistent with SJT, intergroup evaluation on all measures was moderated by self-reported conservatism. Furthermore, the magnitude of these relationships depended on the level of analysis, with small effects emerging at the individual level and medium-sized effects emerging at the social group level. Social group-level analyses also indicated that intergroup evaluation in disadvantaged groups was moderated by stigma. Overall, these findings support and extend the predictions of SJT, but the relatively complex patterns of intergroup evaluation in disadvantaged groups identified here illustrate a need for further theory development and more theory-driven research in this domain. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
Efficient inference of generalized spatial fusion models with flexible specification

In spatial statistics, data are often collected at different spatial resolutions. Often, it is of interest to (a) carry out multivariate analysis when variables are sampled at different locations, (b) model data collected at misaligned areas, or (c) unravel common latent factors by jointly modelling point and areal data. In this paper, we establish a linkage between the generalized spatial fusion model framework and the various change‐of‐support problems, and we outline how the framework can be adapted in these situations. Moreover, we propose an efficient fusion model implementation by exploiting advantages of nearest neighbour Gaussian process and the Stan modelling language. Our simulation shows that the computational efficiency is several times higher in the new implementation compared with original implementation. We illustrate the performance gain in practice using a case study, which models daily precipitation in Switzerland based on rain gauge and radar data.
Illicit drug use and exposure in disadvantaged neighborhoods in Czechia: policy representations and evidence

Abstract This study examines the lifetime prevalence of illicit drug use and illicit drug exposure in disadvantaged (“Roma”) and more affluent neighborhoods in Czechia. The results of a survey among populations of both types of neighborhoods suggest no statistically significant difference between the two in terms of the overall lifetime prevalence of illicit drug use; however, lifetime prevalence of methamphetamine use proved higher in disadvantaged neighborhoods. The population of disadvantaged neighborhoods has also lower chances to use LSD during their lifetime. Further differences were identified in drug exposure, with the population of more affluent neighborhoods being more frequently exposed to illicit drugs than the population of disadvantaged neighborhoods. The predictors of drug use and drug exposure were partially different for both populations. In the disadvantaged population, drug use was revealed, among other predictors, to be associated with housing conditions.
New Insights on the Distribution, Ecology, and Overwintering Behavior of the Little Brown Myotis (Myotis lucifugus) in Alaska

Abstract We initiated the citizen science-based Alaska Bat Monitoring Project in 2004 to investigate the distribution, habitat use, and seasonal ecology of the Little Brown Myotis in Southcentral, Central, and Western Alaska. As of 2012, we received reports of bats from 252 unique locations across the focus area, including Kotzebue, White Mountain, Saint Michael, and the Semidi Islands, which represent significant range extensions for bats in the state. Ninety-seven percent of 111 roosts were located in human structures. Maternity colonies were identified in 48 locations, all in human structures. The majority of observations were reported in late July, August, and September, but we received observations every month of the year. We received reports of bats in 25 unique locations during the winter period from October to April. Winter bats were all associated with buildings unless observed flying outdoors; no hibernacula in natural substrates were documented. Timing and locations of winter observations imply that bats in the most northerly areas are likely non-migratory and overwinter in human structures, while winter observations in Southcentral Alaska suggest both migratory and non-migratory behavior. Despite the limitations and bias inherent in the data set, these reports represent a significant contribution to our understanding of the distribution and ecology of the Little Brown Myotis in Alaska and provide a basis for future directed research efforts.
Distractor intrusions are the result of delayed attentional engagement: A new temporal variability account of attentional selectivity in dynamic visual tasks.

When observers must identify targets among distractors in a rapid serial visual presentation (RSVP) stream, distractor intrusion errors are frequent, demonstrating the difficulty of allocating attention to the right object at the right moment in time. However, the mechanisms responsible for such intrusion errors remain disputed. We propose a new attentional engagement account of selective visual processing in RSVP tasks. Engagement is triggered by the preattentive detection of target-defining features. Critically, the success versus failure of target identification is determined by the speed of such engagement processes on individual trials. To test this account, we measured electrophysiological markers of attentional engagement (N2pc components) in 3 experiments where observers had to report the identity of a target digit in 1 of 2 lateral RSVP streams. On most trials, the target was immediately followed by a digit distractor, resulting in many posttarget distractor intrusions. Critically, N2pcs components measured on distractor intrusion trials were significantly delayed relative to trials with correct target reports. This was the case regardless of whether the target was defined by a shape cue or by its color and even when the location of shape-defined targets was known in advance. These findings show that distractor intrusions are the result of delayed attentional engagement. They demonstrate that temporal variability in attentional selectivity across trials can strongly affect visual awareness and perceptual reports. Our temporal variability account of attentional engagement offers a new framework for assessing the temporal dynamics of attention in visual object recognition. (PsycInfo Database Record (c) 2020 APA, all rights reserved).