Miracle-dz/UniFAR-FTUs huggingface dataset:
Senkyunolide I Protects against Sepsis-Associated Encephalopathy by Attenuating Sleep Deprivation in a Murine Model of Cecal Ligation and Puncture

Sepsis may lead to sleep deprivation, which will promote the development of neuroinflammation and mediate the progression of sepsis-associated encephalopathy (SAE). Senkyunolide I, an active component derived from an herb medicine, has been shown to provide a sedative effect to improve sleep. However, its role in sepsis is unclear. The present study was performed to investigate whether Senkyunolide I protected against SAE in a murine model of cecal ligation and puncture (CLP). Here, we showed that Senkyunolide I treatment improved the 7-day survival rate and reduced the excessive release of cytokines including TNF-α, IL-6, and IL-1β. A fear conditioning test was performed, and the results showed that Senkyunolide I attenuated CLP-induced cognitive dysfunction. Senkyunolide I treatment also decreased the phosphorylation levels of inflammatory signaling proteins, including p-ERK, p-JNK, p-P38, and p-P65, and the level of inflammatory cytokines, including TNF-α, IL-6, and IL-1β, in the hippocampus homogenate. Sleep deprivation was attenuated by Senkyunolide I administration, as demonstrated by the modification of the BDNF and c-FOS expression. When sleep deprivation was induced manually, the protective effect of Senkyunolide I against inflammatory responses and cognitive dysfunction was reversed. Our data demonstrated that Senkyunolide I could protect against sepsis-associated encephalopathy in a murine model of sepsis via relieving sleep deprivation.
APPLICATION OF THE PLACKETT-BURMAN STATISTICAL DESIGN TO OPTIMIZE POLY(BETA-HYDROXYBUTYRATE) PRODUCTION BY RALSTONIA EUTROPHA IN BATCH CULTURE

Ralstonia eutropha accumulates poly(β-hydroxybutyrate) up to 80% of its dry weight (as a carbon andenergy source) and is the best-known PHB producer. Although PHB has many potential applications in medicine, veterinary practice, agriculture and surgery, its high cost limits the widespread use of it. The recentresearches have focused on reducing these costs by optimizing fermentation process. In this study thePlacket Burman experimental design was used to test the relative importance of medium components andprocess variables on cell growth and PHB production. The optimum values of the variables, selected as thebest conditions for further studies in the development of a low cost and effective fermentation process forPHB production, were as follows: initial fructose concentration, 15 g/l; C/N ratio, 7.4; shaking rate, 200 rpm;fermentation time, 40 h; temperature, 30°C; seed age, 15 h .
Immunoinformatic analysis of immunogenic B- and T-cell epitopes of MIC4 protein to designing a vaccine candidate against Toxoplasma gondii through an in-silico approach

Purpose Toxoplasmosis, transmitted by Toxoplasma gondii, is a worldwide parasitic disease that affects approximately one-third of the world's inhabitants. Today, there are no appropriate drugs to deter tissue cysts from developing in infected hosts. So, developing an effective vaccine would be valuable to avoid from toxoplasmosis. Considering the role of microneme antigens such as microneme protein 4 (MIC4) in T. gondii pathogenesis, it can be used as potential candidates for vaccine against T. gondii. Materials and Methods In this study several bioinformatics methods were used to assess the different aspects of MIC4 protein such as secondary and tertiary structure, physicochemical characteristics, the transmembrane domains, subcellular localization, B-cell, helper-T lymphocyte, cytotoxic-T lymphocyte epitopes, and other notable characteristic of this protein design a suitable vaccine against T. gondii. Results The studies revealed that MIC4 protein includes 59 potential post-translational modification sites without any transmembrane domains. Moreover, several probable epitopes of B- and T-cells were detected for MIC4. The secondary structure comprised 55.69% random coil, 5.86% beta-turn, 19.31% extended strand, and 19.14% alpha helix. According to the Ramachandran plot results, 87.42% of the amino acid residues were located in the favored, 9.44% in allowed, and 3.14% in outlier regions. The protein allergenicity and antigenicity revealed that it was non-allergenic and antigenic. Conclusion This study gives vital basic on MIC4 protein for further research and also established an effective vaccine with different techniques against acute and chronic toxoplasmosis.
Nutritional Knowledge and Dietary Intake Habits among Pregnant Adolescents Attending Antenatal Care Clinics in Urban Community in Ghana

Introduction Proper nutrition during pregnancy is important for the wellbeing of the mother and foetus and supports health during pregnancy, delivery, and breastfeeding. However, there are little data on nutritional knowledge and dietary intake among adolescents who are pregnant in Ghana. Hence, the study assessed the nutritional knowledge and eating habits of this vulnerable group in the Ledzokuku-Krowor Municipality, Ghana. Methods The study was cross-sectional and employed a multistage sampling technique to select 423 participants. The study was conducted between October and November 2019. A statistical software was used to analyse data and employed Pearson's chi-square and logistics regression to assess associations between the outcome and predictor variables. A p value <0.05 at a 95% confidence interval was considered statistically significant. Results Less than half (44.9%) of the pregnant adolescents have high nutritional knowledge. About 19.4% of them have good eating habits, while 23.9%, 18.2%, and 6.4% of them do not take breakfast, lunch, and supper, respectively. However, 15.6%, 13.9%, and 9.2% do take snacks after breakfast, lunch, and supper, respectively. About 55.9%, 59.8%, and 23.0% do not take their breakfast, lunch, and supper on time, respectively. Additionally, only 3.8% of them do take fruits and vegetables daily, while 9.7%, 23.2%, 30.0%, and 26.5% of them do take animal products, energy drinks, carbonated drinks, and legumes/nuts/seeds daily, respectively. The study showed that educational level (p=0.014), occupation (p=0.016), ethnicity (p=0.017), and number of pregnancies (p=0.021) were associated with good eating habits. Conclusion Eating habit of adolescent pregnant women was not encouraging. Therefore, the municipal health authority with the concerned stakeholders should intensify efforts, including nutritional education to improve good eating habits, such as taking snacks in between meals, eating on time, and balance diet among pregnant adolescents, and to reduce adolescent pregnancy in the municipality.
A comparative study of haemodynamic effects of single-blinded orotracheal intubations with intubating laryngeal mask airway, Macintosh and McGrath video laryngoscopes

Background The efficacy of an intubation technique is crucial to the success of ventilation therapies. Intubating laryngeal mask airway (ILMA), Macintosh and McGrath techniques are yet to be evaluated thoroughly. Orotracheal intubations with ILMA, McGrath, and Macintosh laryngoscopes are compared based on haemodynamic changes, time taken for intubations, and success rate. Methods This is a prospective, single-blinded, randomised controlled trial. Primary outcome: identification of the most efficient intubation technique. Secondary outcomes: haemodynamic parameters, time taken for intubation and the rate of success of intubation. Patients enrolled: 90. Groups: 3. Each participant is randomly assigned to a group. Inclusion criteria: both sexes, age: 18–55 years, ASA: I or II, Mallampati < III, Mouth opening > 2 fingers, BMI < 40 kg m-2, any elective surgery, general anaesthesia requiring endotracheal intubation. The haemodynamic changes, time taken for intubations, and success rate during ILMA, Macintosh and McGrath intubations were recorded and statistically analysed. Results Macintosh and ILMA raised the heart rate (min-1) more than McGrath at the second minute (95% CI: 76.50 ± 1.34 [McGrath] < 81.73 ± 1.46 [Macintosh] < 90.42 ± 1.24 [ILMA]). ILMA required the longest intubation time (s) (95% CI: 71.64 ± 2.14 [ILMA] > 40.26 ± 1.36 [McGrath] > 30.63 ± 1.53 [Macintosh]). Macintosh and McGrath intubations were all successful, whereas ILMA recorded two failures. However, the observed failures were statistically insignificant (95% CI: 93.33 ± 4.35%). Conclusions McGrath intubation is the most efficient technique based on its haemodynamics. ILMA required the longest intubation time and statistically, rates of success of the techniques are alike.
Guizhi-Shaoyao-Zhimu decoction attenuates bone erosion in rats that have collagen-induced arthritis via modulating NF‐κB signalling to suppress osteoclastogenesis

Abstract Context Guizhi-Shaoyao-Zhimu decoction (GSZD) is commonly used to treat rheumatoid arthritis (RA), but its mechanism is unclear. Objective To investigate the effect of GSZD on bone erosion in type II collagen (CII)-induced arthritis (CIA) in rats and to identify the underlying mechanism. Materials and methods The CIA model was prepared in male Wistar rats by two subcutaneous injections of CII, 1 mg/mL. Fifty CIA rats were randomized equally into the control group given saline daily, the positive group given saline daily and methotrexate 0.75 mg/kg once a week, and three GSZD-treated groups gavaged daily with 800, 1600 and 3200 mg/kg of GSZD for 21 days. GSZD effects were assessed by paw volume, arthritic severity index and histopathology. Cytokine levels were determined by ELISA. The effects of GSZD on RAW264.7 cells were evaluated by receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis and bone resorption assay. Expression of IκB-α and p65 was measured by Western blotting. Major components of GSZD were identified by HPLC. Results Arthritis index score, paw volume and bone destruction score showed that GSZD improved inflammatory symptoms and reduced joint tissue erosion (p < 0.01). GSZD decreased RANKL, and the number of osteoclasts (OCs) in joint tissues (p < 0.01) and increased osteoprotegerin levels (p < 0.01). GSZD inhibited RANKL-induced RAW264.7 differentiation and reduced bone resorption by OCs. GSZD upregulated IκB (p < 0.01) and p65 (p < 0.01) in the cytoplasm and downregulated p65 (p < 0.01) in the cell nucleus. Conclusions Guizhi-Shaoyao-Zhimu decoction has an anti-RA effect, suggesting its possible use as a supplement and alternative drug therapy for RA.
Predictive validity of the braden scale for pressure injury risk assessment in adults: A systematic review and meta‐analysis

Pressure injuries are common adverse events in clinical practice, affecting the well‐being of patients and causing considerable financial burden to healthcare systems. It is therefore essential to use reliable assessment tools to identify pressure injuries for early prevention. The Braden Scale is a widely used tool to assess pressure injury risk, but the literature is currently lacking in determining its accuracy. This study aimed to evaluate the accuracy of the Braden Scale in assessing pressure injury risk.
Plasmon approach to the band gap of alkali halides

Quantitative order of the band gap of Li, Na, K, and Rb halides is calculated using the plasma oscillation theory of solids. The present method is not limited to binary or ternary compounds but can be used for all the semiconducting and insulating compounds. The calculated values are in excellent agreement with the observed and calculated values of different workers. Eine quantitative Einordnung der Bandlucke von Li-, Na-, K- und Rb-Halogeniden wird mit der Theorie der Plasmaschwingungen in Festkorpern berechnet. Die vorgelegte Methode ist nicht auf binare oder ternare Verbindungen beschrankt, sondern kann fur alle halbleitenden und isolierenden Verbindungen benutzt werden. Die berechneten Werte befinden sich in ausgezeichneter Ubereinstimmung mit beobachteten und von verschiedenen anderen Autoren berechneten Werten.
Patterns and Trends of Gun Violence Against Women in the United States

Objective: To examine patterns and trends of firearm injuries in a nationally representative sample of US women. Summary of Background Data: Gun violence in the United States exceeds rates seen in most other industrialized countries. Due to the paucity of data little is known regarding demographics and temporal variations in firearm injuries among women. Methods: Data were extracted from the Centers for Disease Control and Prevention's Web-based Injury Statistics Query and Reporting System (2001–2017) for women 18 years and older. Number of nonfatal firearm assaults and homicide per year were extracted and crude population-based injury rates were calculated. Sub-stratification by age-group and time period were performed. Results: Between 2001 and 2017, there were 88,823 nonfatal firearm assaults involving women and 29,106 firearm homicides. There were 4116 victims of nonfatal firearm assault in 2001 (3.8 per 105) and 12,959 by 2017 (10.0 per 105). Homicide rates were 1.5 per 105 in 2001 and 1.7 per 105 in 2017. Sub-stratification by age-group and time period showed that there were no significant changes in nonfatal firearm assault rates between 2001 and 2010 (P-trend = 0.132 in 18–44 yo; 0.298 in 45–64 yo). However between 2011 and 2017, nonfatal assault rates increased from 7.10 per 105 to 19.24 per 105 in 18–44 yo (P-trend = 0.013) and from 1.48 per 105 to 3.93 per 105 in 45–64 yo (P-trend = 0.003). Similar trends were seen with firearm homicide among 18–44 yo (1.91 per 105 to 2.47 per 105 in 2011–2017, P-trend = 0.022). However, the trends among 45–64 yo were not significant in both time periods. Conclusions: Female victims of gun violence are increasing and more recent years have been marked with higher rates of firearm injuries, particularly among younger women. These data suggest that improved public health strategies and policies may be beneficial in reducing gun violence against US women.
Effects of high fructose diet on lipid metabolism and the hepatic NF-κB/ SIRT-1 pathway

ABSTRACT The liver is the primary site for fructose metabolism; therefore, the liver is susceptible to fructose related metabolic disturbances including metabolic insulin dysfunction, dyslipidemia and inflammation. We investigated whether astaxanthin (ASX) can modify hepatic nuclear factor-kappa B (NF-κB)/sirtuin-1 (SIRT-1) expression to alter oxidative stress caused by ingestion of excess fructose in rats. The animals were divided randomly into two x two factorially arranged groups: two regimens were given either water (W) or 30% fructose in drinking water (F). These two groups were divided further into two subgroups each: two treatments, either orally with 0.2 ml olive oil (OO) or 1 mg ASX/kg/day in 0.2 ml olive oil (ASX). Fructose administration increased serum glucose, triglycerides and very low density lipoproteins, and decreased serum concentration of high density lipoproteins; fructose did not alter serum total cholesterol. Excess fructose decreased hepatic superoxide dismutase (SOD) and increased hepatic NF-κB and MDA levels. ASX treatment increased hepatic SIRT-1 and decreased hepatic NF-κB and malondialdehyde (MDA) levels. ASX treatment decreased hepatic NF-κB and increased SOD levels, but did not alter MDA level in rats fed high fructose. ASX administration ameliorated oxidative stress caused by excess fructose by increasing hepatic NF-κB and SIRT-1 expression.
Stomach Cancer Incidence and Mortality Trends among Circumpolar Nations

Background: Stomach cancer incidence and mortality rates are declining across circumpolar nations, but the burden may not be distributed equally across subpopulations, including Indigenous peoples. Our objective was to examine stomach cancer incidence and mortality trends across circumpolar populations. Methods: Cancer incidence and mortality data from 1999–2016 were obtained from the Canadian Cancer Registry, Canadian Vital Statistics, CDC WONDER, NORDCAN, Northwestern Russian cancer registries, and National Cancer Reports. The direct method was used to calculate 10-year rolling age-standardized incidence and mortality rates to the world (WHO 2000–2025) and 2011 Canadian standard populations. Standardized incidence rate ratios (SRR) were calculated. Data were stratified by sex, year, and region. U.S. data were broken down by race [White; American Indian/Alaska Native (AIAN)]. Race data were not available from non-U.S. cancer registries. Results: Most populations showed declining incidence and mortality rates over time. Incidence rates among Greenland males and females, Alaska AIAN males and females, and Northern Canadian both sexes were elevated compared with regional counterparts and remained stable. The largest male SRR was observed among Alaska AIAN versus Alaska Whites [SRR = 3.82; 95% confidence interval (95% CI), 2.71–5.37]. The largest female SRR was observed among Alaska AIAN versus Alaska Whites (SRR = 4.10; 95% CI, 2.62–6.43). Conclusions: Despite stomach cancer incidence and mortality rates declining overall, some northern and Indigenous populations experience elevated and stable incidence and mortality rates. Impact: There is a need to address disparities observed among circumpolar subpopulations. Given similarities in incidence, mortality, and risk factor prevalence across circumpolar regions, addressing disparities could benefit from coordinated international action.
Reliability of moral decision-making: Evidence from the trolley dilemma

The application of framing effects in the field of moral judgement has offered a golden opportunity to assess the reliability of people’s moral judgements and decisions. To date, however, these studies are still scarce and they suffer from multiple methodological issues. Therefore, this study aims to provide further insights into the reliability of moral judgements while fixing these methodological shortcomings. In this study, we employed the classic trolley dilemma moral decision-making paradigm to determine the extent to which moral decisions are susceptible to framing effects. A total of 1,040 participants were included in the study. The data revealed that choices of participants did not significantly differ between the two frames. Equivalence tests confirmed that the associated effect size was very small. Further exploratory analyses revealed an unplanned interaction between the framing effect and the target of the framing manipulation. This result became from marginally statistically significant to insignificant following different sensitivity analyses. The implications and limitations of these findings and directions for future research are discussed.
Comparison of two inverse planning algorithms for cervical cancer brachytherapy

Abstract Purpose To compare two inverse planning algorithms, the hybrid inverse planning optimization (HIPO) algorithm and the inverse planning simulated annealing (IPSA) algorithm, for cervical cancer brachytherapy and provide suggestions for their usage. Material and methods This study consisted of 24 cervical cancer patients treated with CT image‐based high‐dose‐rate brachytherapy using various combinations of tandem/ovoid applicator and interstitial needles. For fixed catheter configurations, plans were retrospectively optimized with two methods: IPSA and HIPO. The dosimetric parameters with respect to target coverage, localization of high dose volume (LHDV), conformal index (COIN), and sparing of organs at risk (OARs) were evaluated. A plan assessment method which combines a graphical analysis and a scoring index was used to compare the quality of two plans for each case. The characteristics of dwell time distributions of the two plans were also analyzed in detail. Results Both IPSA and HIPO can produce clinically acceptable treatment plans. The rectum D2cc was slightly lower for HIPO as compared to IPSA (P = 0.002). All other dosimetric parameters for targets and OARs were not significantly different between the two algorithms. The generated radar plots and scores intuitively presented the plan properties and enabled to reflect the clinical priorities for the treatment plans. Significant different characteristics were observed between the dwell time distributions generated by IPSA and HIPO. Conclusions Both algorithms could generate high‐quality treatment plans, but their performances were slightly different in terms of each specific patient. The clinical decision on the optimal plan for each patient can be made quickly and consistently with the help of the plan assessment method. Besides, the characteristics of dwell time distribution were suggested to be taken into account during plan selection. Compared to IPSA, the dwell time distributions generated by HIPO may be closer to clinical preference.
Spatial heterogeneity and environmental predictors of permafrost region soil organic carbon stocks

Heterogeneous environmental controls on permafrost region soil organic carbon stocks can now be quantified with spatial modeling. Large stocks of soil organic carbon (SOC) have accumulated in the Northern Hemisphere permafrost region, but their current amounts and future fate remain uncertain. By analyzing dataset combining >2700 soil profiles with environmental variables in a geospatial framework, we generated spatially explicit estimates of permafrost-region SOC stocks, quantified spatial heterogeneity, and identified key environmental predictors. We estimated that Pg C are stored in the top 3 m of permafrost region soils. The greatest uncertainties occurred in circumpolar toe-slope positions and in flat areas of the Tibetan region. We found that soil wetness index and elevation are the dominant topographic controllers and surface air temperature (circumpolar region) and precipitation (Tibetan region) are significant climatic controllers of SOC stocks. Our results provide first high-resolution geospatial assessment of permafrost region SOC stocks and their relationships with environmental factors, which are crucial for modeling the response of permafrost affected soils to changing climate.
Dual-Frequency CMUT Arrays for Multiband Ultrasound Imaging Applications

Dual-frequency capacitive micromachined ultrasonic transducers (CMUTs) are introduced for multiscale imaging applications, where a single array transducer can be used for both deep low-resolution imaging and shallow high-resolution imaging. These transducers consist of low- and high-frequency membranes interlaced within each subarray element. They are fabricated using a modified sacrificial release process. Successful performance is demonstrated using wafer-level vibrometer testing, as well as acoustic testing on wirebonded dies consisting of arrays of 2- and 9-MHz elements of up to 64 elements for each subarray. The arrays are demonstrated to provide multiscale, multiresolution imaging using wire phantoms and can span frequencies from 2 MHz up to as high as 17 MHz. Peak transmit sensitivities of 27 and 7.5 kPa/V are achieved with the low- and high-frequency subarrays, respectively. At 16-mm imaging depth, lateral spatial resolution achieved is 0.84 and 0.33 mm for low- and high-frequency subarrays, respectively. The signal-to-noise ratio of the low-frequency subarray is significantly higher for deep targets compared to the high-frequency subarray. The array achieves multiband imaging capabilities difficult to achieve with current transducer technologies and may have applications to multipurpose probes and novel contrast agent imaging schemes.
The Effect of Oxygen on the Inhibition of Copper Corrosion with Benzotriazole

The inhibition of copper corrosion by benzotriazole (BTAH) has been studied by scanning electrochemical microscopy as a function of time and oxygen content. Under normal atmospheric conditions the inhibiting [Cu(I)-BTA] film was formed in approximately 1 h. When the oxygen content was lowered below 15 ppm, an insulating film could not be obtained in 4 h, clearly showing that the formation of the inhibiting [Cu(I)-BTA] requires oxygen.