Miracle-dz/UniFAR-FTUs huggingface dataset:
Choline acetyltransferase‐like immunoreactivity in the superior colliculus of the cat and its relation to the pattern of acetylcholinesterase staining

Choline acetyltransferase, the biosynthetic enzyme for acetylcholine, is thought to be a marker for cholinergic neurons. This report presents an analysis of the pattern of choline acetyltransferase‐like immunoreactivity in the superior colliculus of the cat, A dense network of highly varicose immunoreactive fibers pervaded the superficial gray and optical layer. The density of the fiber network in the superficial layers was heterogeneous, forming a mosaic pattern with a period of about 400 μm. The antigen was also located in numerous small perikarya embedded in this network. This neuronal population reached a density of 2,000 cells/mm3 of the superficial gray layer and suggested the presence of a substantial cholinergic system originating in the superior colliculus. A detailed comparison was made between the pattern of choline acetyltransferase‐like immunoreactivity and the distribution of acetylcholinesterase activity. By comparisons of adjacent sections, both staining patterns were found to be similar in all collicular layers. In particular, the compartmental distribution of immunoreactivity in the intermediate collicular layers seemed to mimic the pattern of acetylcholinesterase staining. A double‐staining technique demonstrated a near‐perfect correlation between the two patterns. In conclusion, there was no indication of heightened acetylcholinesterase activity without an associated elevation in choline acetyltransferase‐like immunoreactivity throughout the superior colliculus. In this part of the brain, the presence of the putative cholinergic terminals could fully account for the distribution of acetylcholinesterase activity.
Eficacia de los folletos publiciatarios en el comercio detallista

Los folletos publicitarios se han convertido en una de las principales herramientas promocionales utilizadas por el comercio detallista. Sin embargo, no existe un elevado numero de trabajos en la literatura de marketing que analicen su eficacia y su efecto sobre el comportamiento de eleccion del consumidor. Este trabajo pretende cubrir esta escasez de investigaciones analizando el efecto que la presencia de las marcas en los folletos publicitarios tiene en la probabilidad de eleccion del consumidor, y como este efecto se relaciona con caracteristicas del individuo, tales como su sensibilidad al precio o su nivel de lealtad hacia la marca. Para ello, se utiliza la metodologia de los modelos logit y el enfoque de clases latentes a partir de datos de escaner. Los resultados permiten ofrecer sugerencias a los detallistas con objeto de conseguir mejorar la eficacia de esta herramienta promocional.
The Drosophila Toll Signaling Pathway

The identification of the Drosophila melanogaster Toll pathway cascade and the subsequent characterization of TLRs have reshaped our understanding of the immune system. Ever since, Drosophila NF-κB signaling has been actively studied. In flies, the Toll receptors are essential for embryonic development and immunity. In total, nine Toll receptors are encoded in the Drosophila genome, including the Toll pathway receptor Toll. The induction of the Toll pathway by Gram-positive bacteria or fungi leads to the activation of cellular immunity as well as the systemic production of certain antimicrobial peptides. The Toll receptor is activated when the proteolytically cleaved ligand Spatzle binds to the receptor, eventually leading to the activation of the NF-κB factors Dorsal-related immunity factor or Dorsal. In this study, we review the current literature on the Toll pathway and compare the Drosophila and mammalian NF-κB pathways.
Retinoids Inhibit Interleukin-12 Production in Macrophages through Physical Associations of Retinoid X Receptor and NFκB*

Lipopolysaccharide (LPS) increases the production of interleukin-12 (IL-12) from mouse macrophages via a κB site within the IL-12 p40 promoter. In this study, we found that retinoids inhibit this LPS-stimulated production of IL-12 in a dose-dependent manner. The NFκB components p50 and p65 bound retinoid X receptor (RXR) in a ligand-independent manner in vitro, and the interaction interfaces involved the p50 residues 1–245, the p65 residues 194–441, and the N-terminal A/B/C domains of RXR. Activation of macrophages by LPS resulted in markedly enhanced binding activities to the κB site, which significantly decreased upon addition of retinoids, as demonstrated by the electrophoretic mobility shift assays. In cotransfections of CV-1 and HeLa cells, RXR also inhibited the NFκB transactivation in a ligand-dependent manner, whereas a mutant RXR lacking the AF2 transactivation domain, which serves as ligand-dependent binding sites for transcription integrators SRC-1 and p300, was without any effect. In addition, coexpression of increasing amounts of SRC-1 or p300 relieved the retinoid-mediated inhibition of the NFκB transactivation. From these results, we propose that retinoid-mediated suppression of the IL-12 production from LPS-activated macrophages may involve both inhibition of the NFκB-DNA interactions and competitive recruitment of transcription integrators between NFκB and RXR.
QoS Provisioning in Wireless Body Area Networks: A Review on MAC Aspects

Wireless Body Area Networks (WBANs) deal with variety of healthcare services with diverse Quality of Service (QoS) requirements. However, QoS handling is a challenging problem in such networks. In general, QoS related problems can be addressed from different layers in the networking protocol suite. Design of an efficient QoS aware Medium Access Control (MAC) protocol can address this problem in MAC layer. This paper analyzes the QoS requirements of WBAN, identifies the requisites of QoS handling system, and outlines the trends that are being followed for its advancement with focus on QoS issues at MAC layer. We review some prior works, compare them, and analyze the current research concerned with problem of providing QoS in WBAN. We also explore some open issues and discuss them.
DialBetics With a Multimedia Food Recording Tool, FoodLog

Background: Diabetes self-management education is an essential element of diabetes care. Systems based on information and communication technology (ICT) for supporting lifestyle modification and self-management of diabetes are promising tools for helping patients better cope with diabetes. An earlier study had determined that diet improved and HbA1c declined for the patients who had used DialBetics during a 3-month randomized clinical trial. The objective of the current study was to test a more patient-friendly version of DialBetics, whose development was based on the original participants’ feedback about the previous version of DialBetics. Method: DialBetics comprises 4 modules: data transmission, evaluation, exercise input, and food recording and dietary evaluation. Food recording uses a multimedia food record, FoodLog. A 1-week pilot study was designed to determine if usability and compliance improved over the previous version, especially with the new meal-input function. Results: In the earlier 3-month, diet-evaluation study, HbA1c had declined a significant 0.4% among those who used DialBetics compared with the control group. In the current 1-week study, input of meal photos was higher than with the previous version (84.8 ± 13.2% vs 77.1% ± 35.1% in the first 2 weeks of the 3-month trial). Interviews after the 1-week study showed that 4 of the 5 participants thought the meal-input function improved; the fifth found input easier, but did not consider the result an improvement. Conclusions: DialBetics with FoodLog was shown to be an effective and convenient tool, its new meal-photo input function helping provide patients with real-time support for diet modification.
A novel pH-responsive hydrogel-based on calcium alginate engineered by the previous formation of polyelectrolyte complexes (PECs) intended to vaginal administration

Abstract This work aimed to develop a calcium alginate hydrogel as a pH responsive delivery system for polymyxin B (PMX) sustained-release through the vaginal route. Two samples of sodium alginate from different suppliers were characterized. The molecular weight and M/G ratio determined were, approximately, 107 KDa and 1.93 for alginate_S and 32 KDa and 1.36 for alginate_V. Polymer rheological investigations were further performed through the preparation of hydrogels. Alginate_V was selected for subsequent incorporation of PMX due to the acquisition of pseudoplastic viscous system able to acquiring a differential structure in simulated vaginal microenvironment (pH 4.5). The PMX-loaded hydrogel (hydrogel_PMX) was engineered based on polyelectrolyte complexes (PECs) formation between alginate and PMX followed by crosslinking with calcium chloride. This system exhibited a morphology with variable pore sizes, ranging from 100 to 200 μm and adequate syringeability. The hydrogel liquid uptake ability in an acid environment was minimized by the previous PECs formation. In vitro tests evidenced the hydrogels mucoadhesiveness. PMX release was pH-dependent and the system was able to sustain the release up to 6 days. A burst release was observed at pH 7.4 and drug release was driven by an anomalous transport, as determined by the Korsmeyer–Peppas model. At pH 4.5, drug release correlated with Weibull model and drug transport was driven by Fickian diffusion. The calcium alginate hydrogels engineered by the previous formation of PECs showed to be a promising platform for sustained release of cationic drugs through vaginal administration.
Pax‐5 and EBF are Expressed in Committed B‐Cell Progenitors Prior to the Colonization of the Embryonic Bursa of Fabricius*

The committed B‐cell precursors developing from hemopoietic stem cells have been considered to differentiate through a common lymphoid progenitor stage in the mouse. In the chicken B‐cell system, however, the committed B‐cell progenitors burst as a single wave prior to the bursal colonization and most likely as direct descendants of hemopoietic stem cells. In the present report we show that prebursally committed B‐cell progenitors specifically express early B‐cell factor (EBF) and Pax‐5 transcription factors. In addition we show that the expression of these and other B‐lineage‐associated transcription factors starts early in the chicken ontogeny. Altogether our findings strongly support the model of early delineation of B‐ and T‐cell development and do not support the existence of common lymphoid stem cells.
Stuck in School: How Social Context Shapes School Choice for Inner-City Students

Background High school choice policies attempt to improve the educational outcomes of poor and minority students by allowing access to high school beyond neighborhood boundaries. These policies assume that given a choice, families will be able to select a school that supports their child's learning and promotes educational attainment. However, research on the effects of public school choice programs on the academic achievement of disadvantaged students is mixed, suggesting that families do not necessarily respond to these programs in ways that policymakers intend. Purpose The purpose of this article is to identify how family and neighborhood contexts interact with public school choice policies to shape the educational opportunities of inner-city students. Specifically, we ask: What criteria are used to choose schools? What are the implications of these school choice decisions for students’ future educational and occupational opportunities? Research Design We use data from interviews and fieldwork conducted with 118 low-income African American youth ages 15–24 who attended Baltimore City Public Schools at some point during their high school career. Research on school choice tends to rely on data from parents, and we offer a unique contribution by asking youth themselves about their experiences with school choice. Conclusions Although school choice policies assume that parents will guide youths’ decision about where to go to high school, the majority of youth in our sample were the primary decision makers in the high school choice process. Additionally, these youth made these choices under considerable constraints imposed by the district policy and by their family, peers, and academic background. As a result, the youth often selected a school within a very limited choice set and chose schools that did not necessarily maximize their educational opportunity. Our results demonstrate that school choice policies must take into account the social context in which educational decisions are made in order to maximize chances for students’ individual academic achievement and to decrease inequality by race and social class.
Protein Synthesis in Embryos of Dormant and Germinating Agrostemma githago L. seeds.

The time course of protein synthesis in embryos of dormant and afterripened Agrostemma githago seeds was studied. In embryos of afterripened geminating seeds, protein synthesis increased in three suc- cessive stages: (a) concurrent with swelling; (b) during the lag phase between the completion of water uptake and the onset of growth; and (c) immediately after protrusion through the seed coat. Embryos of dormant seeds showed the first increase but not the second unless dormancy was broken by imbibition at 4°C. This indicates that dormancy affects proc- esses prior to the onset of growth. The third increase was largely due to higher oxygen availability after the rupture of the seed coat and not to actual growth. It could also be elicited in dormant embryos by isolating them from the seeds. Electrophoretic analysis of the newly synthesized proteins demon- strated that the patterns of dormant and afterripened embryos became significantly different in both axes and cotyledons only just prior to the onset of axis elongation. Thereafter, the differences became larger. When afterripened or dormant seeds were transferred from a low, germination-permitting to a high, germination-inhibiting temperature, the seeds germinated at the high temperature if they had completed the lag phase to a sufficient extent at the low temperature. This shows that the processes during the lag phase were inhibited by the high temperature while the onset of growth was not affected.
Synovitis and Osteitis Are Very Frequent in Rheumatoid Arthritis Clinical Remission: Results from an MRI Study of 294 Patients in Clinical Remission or Low Disease Activity State

Objective. In rheumatoid arthritis (RA), radiographic progression may occur despite clinical remission. This may be explained by subclinical inflammation. Magnetic resonance imaging (MRI) provides a greater sensitivity than clinical examination and radiography for assessing disease activity. Our objective was to determine the MRI characteristics of RA patients in clinical remission or low disease activity (LDA) state. Methods. Databases from 6 cohorts were collected from 5 international centers. RA patients in clinical remission according to Disease Activity Score28-C-reactive protein (DAS28-CRP < 2.6; n = 213) or LDA-state (2.6 ≤ DAS28-CRP < 3.2; n = 81) with available MRI data were included. MRI were assessed according to the OMERACT RA MRI scoring system (RAMRIS). Results. Patient characteristics: 70% women, median age 55 (interquartile range, IQR 43–63) years, disease duration 2.3 (IQR 0.7–5.1) years, DAS28-CRP 2.2 (IQR 1.8–2.6), Simplified Disease Activity Index, SDAI, 3.9 (IQR 1.9–6.5), Clinical Disease Activity Index, CDAI, 3.1 (IQR 1.5– 5.8), rheumatoid factor/anti-cyclic citrullinated peptide positivity 57%/54%, presence of radiographic erosions: 66%. Wrist and metacarpophalangeal MRI (MCP-MRI) data were available for 287 and 241 patients, respectively. MRI inflammatory activity in wrist and/or MCP joints was observed in the majority [synovitis: 95%, bone edema (osteitis): 35%] of patients. The median (IQR) RAMRIS score was 6 (3–9) for synovitis and 0 (0–2) for osteitis. Synovitis and osteitis were not less frequent in DAS28 clinical remission (synovitis/osteitis 96%/35%) than LDA (91/36). A trend towards lower frequencies of osteitis in patients in SDAI and CDAI remission was observed. Conclusion. Subclinical inflammation was identified by MRI in the majority of RA patients in clinical remission or LDA state. This may explain structural progression in such patients. Further work is required to understand the place of modern imaging in future remission criteria.
Sustained high-titer antibody responses induced by conjugating a malarial vaccine candidate to outer-membrane protein complex

The development of protein subunit vaccines to combat some of the world's deadliest pathogens such as a malaria parasite, Plasmodium falciparum, is stalled, due in part to the inability to induce and sustain high-titer antibody responses. Here, we show the induction of persistent, high-titer antibody responses to recombinant Pfs25H, a human malarial transmission-blocking protein vaccine candidate, after chemical conjugation to the outer-membrane protein complex (OMPC) of Neisseria meningitidis serogroup B and adsorption to aluminum hydroxyphosphate. In mice, the Pfs25H-OMPC conjugate vaccine was >1,000 times more potent in generating anti-Pfs25H ELISA reactivity than a similar 0.5-μg dose of Pfs25H alone in Montanide ISA720, a water-in-oil adjuvant. The immune enhancement requires covalent conjugation between Pfs25H and the OMPC, given that physically mixed Pfs25H and OMPC on aluminum hydroxyphosphate failed to induce greater activity than the nonconjugated Pfs25H on aluminum hydroxyphosphate. The conjugate vaccine Pfs25H-OMPC also was highly immunogenic in rabbits and rhesus monkeys. In rhesus monkeys, the antibody responses were sustained over 18 months, at which time another vaccination with nonconjugated Pfs25H induced strong anamnestic responses. The vaccine-induced anti-Pfs25-specific antibodies in all animal species blocked the transmission of parasites to mosquitoes. Protein antigen conjugation to OMPC or other protein carrier may have general application to a spectrum of protein subunit vaccines to increase immunogenicity without the need for potentially reactogenic adjuvants.
The Association of Bone Mineral Density and G2014A Polymorphism in the Estrogen Receptor Alpha Gene in Osteoporotic Hip Fracture in Thai Population.

OBJECTIVE This study aimed to determine the association of a recent identified G2014A single nucleotide polymorphism (SNP) genotype distribution in exon 8 of the estrogen receptor in postmenopausal Thai women. MATERIAL AND METHOD A prospective study was conducted at Ramathibodi Hospital between July 2005 and July 2006. Postmenopausal Thai women, aged more than 55 years and had sustained osteoporotic hipfracture, were included. Exclusion criteria were renal and metabolic bone diseases. Age, body mass index (BMI), blood tests for metabolic bone disease, and G2014A SNP genotype, bone mineral density (BMD) were collected. The relationship between the degree of osteoporosis (normal, osteopenia, and osteoporosis) and SNP genotype was analyzed by Fisher's exact test. RESULTS Sixty-five postmenopausal women with osteoporosis were included. The average age was 76.2 ± 10.9 years old, and the average BMI was 21.3 ± 3.5 kg/m2. The data expressing the genotype distribution of gene G2014A SNP were G/G 23.1%, G/A 29.2% and A/A 47.7%. There was no statistical difference between age and BMI in each genotype. Gene G2014A was associated with osteoporosis of lumbar spine, femoral neck, ward triangle, and femoral neck. CONCLUSION It could be concluded that a G2014A SNP genotype in exon 8 of the estrogen receptor was associated with postmenopausal women who had osteoporotic hip fracture.
Three-dimensional conformal radiotherapy in combination with transcatheter arterial chemoembolization in the treatment of hepatocellular carcinoma.

PURPOSE This study aimed to investigate the efficacy and safety of transcatheter arterial chemoembolization (TACE) in combination with three-dimensional conformal radiotherapy (3D-CRT) in the treatment for locally advanced unresectable hepatocellular carcinoma (HCC). METHODS From March 2000 to March 2009 a total of 158 patients with unresectable HCC treated and followed at our hospital were divided into TACE group (N=80) and TACE combined with 3D-CRT group (N=78). The TACE group was treated 3-6 times. In the combination group, 2-3 TACE courses were administered and 3D-CRT was performed 2 weeks after the last TACE. Three months after the end of treatment, imaging and serum AFP were carried out to assess the treatment efficacy. RESULTS The response rates of TACE and the combination groups were 53.7% (43/80) and 71.8% (58/78) (p<0.05). The 1, 2, and 3-year survival rates of patients in the TACE and combination groups were 58.75, 36.25, 16.25%, and 78.48, 55.12 and 25.64% (p<0.05), respectively. Treatment compliance was good, with at least 2 TACE administrations for each case and at least 52 Gy for radiotherapy. In the TACE and the combination group, there were 2 and 3 cases with grade III/IV toxicity, respectively, without treatment-related death. CONCLUSION 3D-CRT in combination with TACE significantly improve the therapeutic outcome in patients with locally advanced unresectable HCC, without creating severe toxicity.
Peas grown in media with elevated plant-available silicon levels have higher activities of chitinase and β-1,3-glucanase, are less susceptible to a fungal leaf spot pathogen and accumulate more foliar silicon

Pea seedlings grown for 5 weeks in a growing medium amended with potassium silicate fertiliser had significantly greater activities of the enzymes chitinase and β-1,3-glucanase in leaf extracts compared with those grown in coir-based control mix, prior to challenge by a foliar pathogen. Additionally, fewer lesions developed on pea leaves inoculated with the fungal pathogen Mycosphaerella pinodes 5 weeks after growth in the Si-amended mix. Plant-available Si, as monosilicic acid, in the Si-amended mix was more than five times that in the control mix. Peas accumulated nearly twice as much silicon (w/w dried foliage) after 3 weeks growth in an alternative potting mix amended with silicon from rice hull derivatives, as those grown in a conventional perlite/sand medium as control. The monosilicic acid content of the rice hull mix was approximately three times greater than that of the perlite/sand mix. There were no significant differences in dry weights between plants from control and Si-amended mixes. The results suggest associations in pea between available silicon in growth media, accumulation of Si within the plant, early activation of host defences and subsequent resistance to fungal pathogens, with potential for reduction and control of diseases.