Miracle-dz/UniFAR-FTUs huggingface dataset:
Teenage pregnancy in Africa: Trend and Determinants in the 21st Century

Background: Africa remains one of the continents with the highest levels of teenage pregnancies in the world. In spite of this, there are limited empirical research studies on determinants of teenage pregnancy in Africa. This study aims to investigate the trend and determinants of teenage pregnancy in Africa. Several social and economic factors appear to be the causes of teenage pregnancy in Africa. Therefore, understanding the association between teenage pregnancy and various social and economic factors would help reduce teenage pregnancy rate in Africa. Methods: Data sets from the World Bank Organisation of all Africa countries between 2000 and 2010 were obtained to conduct this study. The trends of average teenage pregnancy rate across all regions were examined using descriptive method. Also, the association between teenage pregnancy rate and various economic and social factors were investigated using multivariate statistics methods. Results: In all 52 countries examined there has been a significant reduction in the African teenage pregnancy rate between 2000 and 2010. In addition, correlation analysis carried out showed an inverse significant relationship with life expectancy, literacy rate and contraceptive prevalence. Further analysis reveals that female literacy rate is the most important predictor of teenage pregnancy in Africa. Conclusion: The findings of this research indicate that social and economic factors are important predictors of teenage pregnancy rate in Africa. Evidence from this study suggests that a practical approach to reducing the current teenage pregnancy rate is to develop strategies and policies that support and promotes female literacy.
Association of glaucoma with risk of retinal vein occlusion: A meta‐analysis

To summarize epidemiological evidences on the association between glaucoma and the risk of retinal vein occlusion (RVO). Relevant studies were identified by searching in PubMed, EMBASE and Cochrane until February 2018. Fifteen eligible observational studies were aggregated in this analysis. All results were analysed and pooled using random effects models with 95% confidence intervals (CI). In all studies, the odds ratio (OR) of glaucoma as a risk factor for RVO was 4.01 (95% CI: 3.28–4.91). In RVO subtype‐differentiated subgroup analyses (six studies), the pooled OR showed that glaucoma was associated with central retinal vein occlusion (CRVO) (OR: 6.21; 95% CI: 4.64–8.31), branch retinal vein occlusion (BRVO) (OR: 2.38; 95% CI: 1.77–3.19) and hemiretinal vein occlusion (HRVO) (OR: 4.60; 95% CI: 2.26–9.35). In glaucoma‐classified subgroup analyses (five studies), primary open‐angle glaucoma (POAG) (OR: 5.03; 95% CI: 3.97–6.37) and chronic open‐angle glaucoma (COAG) (OR: 2.36; 95% CI: 1.39–4.02) were significant risk factors for RVO development. There was a plausible relationship between primary angle closure glaucoma (PACG) and RVO risk (OR: 1.85; 95% CI: 0.41–8.35); to be precise, the OR was 5.3 in PACG and CRVO risk (95% CI: 1.04–26.95; p = 0.045), while the OR was 0.65 in PACG and BRVO risk (95% CI: 0.07–6.27; p = 0.707). To sum up, this meta‐analysis shows that glaucoma is associated with the risk of RVO. Glaucoma should be kept in mind when investigating patients with RVO in the clinic.
The IgG Subclass Pattern of Complement Activation Depends on Epitope Density and Antibody and Complement Concentration

Using a matched series of human‐mouse chimaeric IgG anti‐5‐iodo‐4‐hydroxy‐3‐nitrophenacetyl (anti‐NIP) antibodies and NIP‐bovine serum albumin (NIP‐BSA) we examined how different variables influence the activation pattern of complement. When BSA was used with different hapten densities and the input of NIP‐BSA was altered, we observed a change in the subclass reaction pattern. IgG3 fixed more C1q than IgG1 and IgG2 in all situations. IgG1 was slightly better than IgG3 and IgG2 at high antigen concentrations at activating C4 and C3 and inducing formation of the terminal complement complex (TCC) When the epitope density and/or the NIP‐BSA concentration was reduced, IgG3 became best, followed by IgG1 and IgG2. IgG1 now revealed a marked prozone. Furthermore, IgG4 was found to activate C3 and mediate TCC formation at high epitope and complement concentrations.
Antibiotic prescribing trends among US dermatologists in Medicare from 2013 to 2016

Abstract Objectives: Antibiotics are commonly used in dermatology for infectious and inflammatory diseases, and dermatologists prescribe the most antibiotics per physician of any specialist in the United States (US). While oral antibiotics are effective for many conditions, adverse effects and increase in resistance are important public health issues. The purpose of this study is to examine trends in oral antibiotic prescription by US dermatologists using Medicare claims. Materials and Methods: Retrospective review using publicly available Medicare Part D prescriber public use files from 2013 to 2016. Results: The number of dermatologists within the Medicare system increased from 10,210 to 10,749. The proportion of prescriptions by dermatologists that were oral antibiotics increased from 10.0% to 10.7% (p = .023). The use of oral antibiotics increased 10.1% (2.9 claims per 1,000 beneficiaries) from 28.7 to 31.6 claims per 1,000 beneficiaries. There were more oral antibiotic prescriptions in the South than in other regions. Doxycycline was the most commonly prescribed antibiotic each year in all regions. Conclusions: Between 2013 and 2016, the number of oral antibiotics prescribed by dermatologists has increased among Medicare patients. Dermatologists must continue to reevaluate antibiotic prescription practices, particularly among the elderly population, in order to provide optimal care to patients.
Effectiveness of sigmoidoscopy for assessing ulcerative colitis disease activity and therapeutic response

Abstract Ulcerative colitis (UC) typically begins in the rectum and progresses proximally in a contiguous fashion without skip lesions. Post-treatment inflammation distribution can change over time. Colonoscopy is unpleasant for the patient and clinical trials often use sigmoidoscopy for evaluation of disease severity. The aim of this study is to evaluate whether sigmoidoscopy is adequate to assess disease activity and therapeutic response as colonoscopy. We retrospectively reviewed patients who underwent colonoscopy for the initial diagnosis and follow-up by evaluating their mucosal inflammation in our hospital from January 2012 and December 2017. A total of 69 patients were analyzed. During follow up, the inflamed segment changed post-treatment in 62% (43/69). Extensive UC was common in the changed disease extent group (P < .01). Patients treated with oral mesalazine had a higher rate of changed disease extent (P < .01). The sigmoid segment was the most commonly involved segment, and the rectum was the severely inflamed segment during initial diagnosis and follow-up. According to Mayo endoscopic subscore (MES) in the most severely inflamed colonic and rectosigmoid segment, there were high degrees of correlation in the initial UC diagnosis (r = .90, P < .01) and follow-up (r = .74, P < .01). Our findings suggest that sigmoidoscopy is effective as colonoscopy for detecting disease activity and evaluating therapeutic response in UC patients during follow-up.
Isolated adrenocorticotropic hormone deficiency presenting with delirium.

TO THE EDITOR: Adrenocortical insufficiency causes various physical symptoms, including weakness, fatigue, and loss of appetite due to cortisol deficiency. It is also known to cause psychiatric symptoms such as depression or apathy. However, there are only a few reports of delirium associated with this insufficiency (1). We present a case of delirium, which was caused by secondary adrenocortical insufficiency because of isolated adrenocorticotropic hormone deficiency.
Superposition of horseshoe‐like periodicity and linear tonotopic maps in auditory cortex of the Mongolian gerbil

The segregation of an individual sound from a mixture of concurrent sounds, the so‐called cocktail‐party phenomenon, is a fundamental and largely unexplained capability of the auditory system. Speaker recognition involves grouping of the various spectral (frequency) components of an individual's voice and segregating them from other competing voices. The important parameter for grouping may be the periodicity of sound waves because the spectral components of a given voice have one periodicity, viz. fundamental frequency, as their common denominator. To determine the relationship between the representations of spectral content and periodicity in the primary auditory cortex (AI), we used optical recording of intrinsic signals and electrophysiological mapping in Mongolian gerbils (Meriones unguiculatus). We found that periodicity maps as an almost circular gradient superimposed on the linear tonotopic gradient in the low frequency part of AI. This geometry of the periodicity map may imply competitive signal processing in support of the theory of ‘winner‐takes‐all’.
Mesostructural Modeling of Dynamic Modulus and Phase Angle Master Curves of Rubber Modified Asphalt Mixture

The main objective of this paper was to develop a mesostructure-based finite element model of rubber modified asphalt mixture to predict both the dynamic modulus master curve and phase angle master curve under a large frequency range. The asphalt mixture is considered as a three-phase material consisting of aggregate, asphalt mortar, and air void. The mesostructure of the asphalt mixture was digitized by a computed tomography (CT) scan and implemented into finite element software. The 2S2P1D model was used to obtain the viscoelastic information of an asphalt mortar under a large range of frequencies and temperatures. The continuous spectrum of the 2S2P1D model was converted to a discrete spectrum and characterized by the generalized Maxwell model for numerical simulation. The Prony series parameters of the generalized Maxwell model and the elastic modulus of the aggregates were inputted into the finite element analysis as material properties. The dynamic modulus tests of a rubber modified asphalt mortar and asphalt mixture were conducted under different temperatures and loading frequencies. The dynamic modulus master curve and phase angle master curve of both asphalt mortar and asphalt mixture were constructed. The frequency of the finite element simulations of the dynamic modulus tests ranged from 10−6 to 104. The dynamic modulus and phase angle of the asphalt mixture was calculated and the master curves were compared with the master curves obtained from the experimental data. Furthermore, the effect of the elastic modulus of aggregates on the master curves was analyzed. Acceptable agreement between dynamic modulus master curves obtained from experimental data and simulation results was achieved. However, large errors between phase angle master curves appeared at low frequencies. A method was proposed to improve the prediction of the phase angle master curve by adjusting the equilibrium modulus of the asphalt mortar.
Heat Stress-Responsive Transcriptome Analysis in the Liver Tissue of Hu Sheep

Heat stress has a severe effect on animal health and can reduce the productivity and reproductive efficiency; it is therefore necessary to explore the molecular mechanism involved in heat stress response, which is helpful for the cultivation of an animal breed with resistance to heat stress. However, little research about heat stress-responsive molecular analysis has been reported in sheep. Therefore, in this study, RNA sequencing (RNA-Seq) was used to investigate the transcriptome profiling in the liver of Hu sheep with and without heat stress. In total, we detected 520 and 22 differentially expressed mRNAs and lncRNAs, respectively. The differentially expressed mRNAs were mainly associated with metabolic processes, the regulation of biosynthetic processes, and the regulation of glucocorticoid; additionally, they were significantly enriched in the heat stress related pathways, including the carbon metabolism, the PPAR signaling pathway, and vitamin digestion and absorption. The co-located differentially expressed lncRNA Lnc_001782 might positively influence the expression of the corresponding genes APOA4 and APOA5, exerting co-regulative effects on the liver function. Thus, we made the hypothesis that Lnc_001782, APOA4 and APOA5 might function synergistically to regulate the anti-heat stress ability in Hu sheep. This study provides a catalog of Hu sheep liver mRNAs and lncRNAs, and will contribute to a better understanding of the molecular mechanism underlying heat stress responses.
De novo discovery of antibody drugs – great promise demands scrutiny

ABSTRACT We live in an era of rapidly advancing computing capacity and algorithmic sophistication. “Big data” and “artificial intelligence”find progressively wider use in all spheres of human activity, including healthcare. A diverse array of computational technologies is being applied with increasing frequency to antibody drug research and development (R&D). Their successful applications are met with great interest due to the potential for accelerating and streamlining the antibody R&D process. While this excitement is very likely justified in the long term, it is less likely that the transition from the first use to routine practice will escape challenges that other new technologies had experienced before they began to blossom. This transition typically requires many cycles of iterative learning that rely on the deconstruction of the technology to understand its pitfalls and define vectors for optimization. The study by Vasquez et al. identifies a key obstacle to such learning: the lack of transparency regarding methodology in computational antibody design reports, which has the potential to mislead the community efforts
Physical activity, sedentary behavior, and long-term cardiovascular risk in individuals with rheumatoid arthritis

ABSTRACT Objective: Individuals with rheumatoid arthritis (RA) have increased risk of cardiovascular disease (CVD). Lifestyle factors such as prolonged sedentary behavior (SB) and reduced physical activity (PA) may heighten the risk of CVD. The objective of the study was to investigate the role of SB and PA as predictors for long-term CVD risk in RA patients. Methods: A subsample of 273 people diagnosed with RA was extracted from the 2003–2006 National Health and Nutrition Examination Survey and included in this cross-sectional study. Valid accelerometry data were categorized into sedentary behavior, very light, light, and moderate-to-vigorous physical activity. Functional limitations were assessed using a physical function questionnaire. The Framingham risk score (FRS) was used to calculate 10-year CVD risk. Regression models were used to examine the relationships between SB, PA, and 10-year CVD risk while controlling for potential confounders. Results: Participants spent an average of 9 h/day sedentary, 4 h in very light PA, 1 h in light PA, and 0.4 h in moderate-to-vigorous PA. Greater sedentary time was associated with higher 10-year CVD risk (p= 0.019). Increased daily PA, at all intensities, was inversely associated with 10-year CVD risk (p< 0.01). In the fully adjusted regression model, associations between 10-year CVD risk and SB (β = 0.31, R2 = 0.27, p< 0.01), very light PA (β = −0.19, R2 = 0.26, p< 0.01), light PA (β = −0.16, R2 = 0.25, p< 0.01), and moderate-to-vigorous PA (β = −0.15, R2 = 0.25, p< 0.01) remained significant. Conclusions: Strategies for decreasing SB and increasing PA should be explored with individuals with RA in order to decrease long-term CVD risk.
MiR-150-5p retards the progression of myocardial fibrosis by targeting EGR1

ABSTRACT To investigate the differential expression of microRNA-150-5p (miR-150-5p) and early growth response 1 (EGR1) in myocardial fibrosis (MF) cells, and determine the effect between miR-150-5p and EGR1 on MF. Human MF cells were generated via Trypanosoma cruzi (T. cruzi) infection, a mouse model of MF was generated via angiotensin II. The expression levels of miR-150-5p and EGR1 were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot assay. The correlation between miR-150-5p and EGR1 was confirmed by a luciferase reporter assay. The viability, proliferation, and apoptotic rate were detected by cell counting kit-8 (CCK-8), colony-formation and flow cytometry assays. Hematoxylin-eosin (HE) staining and Masson staining visualized the degree of MF. Echocardiography was performed to obtain the levels of left ventricle fractional shortening (LVFS) and left ventricle ejection fraction (LVEF), computer algorithms and a videographics program were used to obtain the levels of left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP) and ±left ventricular dp/dt maximum (LV dp/dtmax). We found that the expression of miR-150-5p in MF cells was lower than normal cardiomyocytes, while the expression level of EGR1 in MF cells were higher than normal cardiomyocytes. Cell experiments demonstrated that EGR1 and miR-150-5p could influence the development of MF, and the expression of EGR1 in cardiomyocytes was regulated by miR-150-5p directly. Lastly, we confirmed that sh-Egr1 would decrease the severity of MF, while miR-150-5p antagomir could aggravate MF. Our results illustrate the mechanism of MF development, and provide a potential target for MF treatment.
The Use of Vowel Length in Making Voicing Judgments by Native Listeners of English and Spanish: Implications for Rate Normalization

Among other characteristics, voiced and voiceless consonants differ in voice onset time (VOT; Lisker & Abramson, 1964). In addition, in English, voiced consonants are typically followed by longer vowels than their unvoiced counterparts (Allen & Miller, 1999). In Spanish, this relationship is less systematic (Zimmerman & Sapon, 1958). In two experiments, we investigated perceptual sensitivities of English and Spanish native speakers to following vowel length (VL) in categorizing syllables that ranged from a prevoiced bilabial stop [ba] to a long-lag bilabial stop [pa]. According to our results, English speakers show sensitivity to following vowels with VLs falling within an English-typical range (Experiment 1), but not when vowels are shorter and in a Spanish-typical range (Experiment 2). Interestingly, Spanish native speakers do not show sensitivity to following VL in either condition. These results suggest that VOT-VL tradeoffs in perception reflect phonological sensitivities of listeners and are not reducible to speech rate compensation.
Altered Standards of Care During an Influenza Pandemic: Identifying Ethical, Legal, and Practical Principles to Guide Decision Making

ABSTRACT Although widespread support favors prospective planning for altered standards of care during mass casualty events, the literature includes few, if any, accounts of groups that have formally addressed the overarching policy considerations at the state level. We describe the planning process undertaken by public health officials in the Commonwealth of Massachusetts, along with community and academic partners, to explore the issues surrounding altered standards of care in the event of pandemic influenza. Throughout 2006, the Massachusetts Department of Public Health and the Harvard School of Public Health Center for Public Health Preparedness jointly convened a working group comprising ethicists, lawyers, clinicians, and local and state public health officials to consider issues such as allocation of antiviral medications, prioritization of critical care, and state seizure of private assets. Community stakeholders were also engaged in the process through facilitated discussion of case scenarios focused on these and other issues. The objective of this initiative was to establish a framework and some fundamental principles that would subsequently guide the process of establishing specific altered standards of care protocols. The group collectively identified 4 goals and 7 principles to guide the equitable allocation of limited resources and establishment of altered standards of care protocols. Reviewing and analyzing this process to date may serve as a resource for other states. (Disaster Med Public Health Preparedness. 2009;3(Suppl 2):S132–S140)
Functional and Structural Divergence in Human TRPV1 Channel Subunits by Oxidative Cysteine Modification*

Transient receptor potential vanilloid 1 (TRPV1) channel is a tetrameric protein that acts as a sensor for noxious stimuli such as heat and for diverse inflammatory mediators such as oxidative stress to mediate nociception in a subset of sensory neurons. In TRPV1 oxidation sensing, cysteine (Cys) oxidation has been considered as the principle mechanism; however, its biochemical basis remains elusive. Here, we characterize the oxidative status of Cys residues in differential redox environments and propose a model of TRPV1 activation by oxidation. Through employing a combination of non-reducing SDS-PAGE, electrophysiology, and mass spectrometry we have identified the formation of subunit dimers carrying a stable intersubunit disulfide bond between Cys-258 and Cys-742 of human TRPV1 (hTRPV1). C258S and C742S hTRPV1 mutants have a decreased protein half-life, reflecting the role of the intersubunit disulfide bond in supporting channel stability. Interestingly, the C258S hTRPV1 mutant shows an abolished response to oxidants. Mass spectrometric analysis of Cys residues of hTRPV1 treated with hydrogen peroxide shows that Cys-258 is highly sensitive to oxidation. Our results suggest that Cys-258 residues are heterogeneously modified in the hTRPV1 tetrameric complex and comprise Cys-258 with free thiol for oxidation sensing and Cys-258, which is involved in the disulfide bond for assisting subunit dimerization. Thus, the hTRPV1 channel has a heterogeneous subunit composition in terms of both redox status and function.
Default Activation and Nuclear Translocation of the Plant Cellular Energy Sensor SnRK1 Regulate Metabolic Stress Responses and Development

Plants appear to have modified the ancient and highly conserved eukaryotic energy sensor to better fit their unique lifestyle and more effectively cope with changing environmental conditions. Energy homeostasis is vital to all living organisms. In eukaryotes, this process is controlled by fuel gauging protein kinases: AMP-activated kinase in mammals, Sucrose Non-Fermenting1 (SNF1) in yeast (Saccharomyces cerevisiae), and SNF1-related kinase1 (SnRK1) in plants. These kinases are highly conserved in structure and function and (according to this paradigm) operate as heterotrimeric complexes of catalytic-α and regulatory β- and γ-subunits, responding to low cellular nucleotide charge. Here, we determined that the Arabidopsis (Arabidopsis thaliana) SnRK1 catalytic α-subunit has regulatory subunit-independent activity, which is consistent with default activation (and thus controlled repression), a strategy more generally used by plants. Low energy stress (caused by darkness, inhibited photosynthesis, or hypoxia) also triggers SnRK1α nuclear translocation, thereby controlling induced but not repressed target gene expression to replenish cellular energy for plant survival. The myristoylated and membrane-associated regulatory β-subunits restrict nuclear localization and inhibit target gene induction. Transgenic plants with forced SnRK1α-subunit localization consistently were affected in metabolic stress responses, but their analysis also revealed key roles for nuclear SnRK1 in leaf and root growth and development. Our findings suggest that plants have modified the ancient, highly conserved eukaryotic energy sensor to better fit their unique lifestyle and to more effectively cope with changing environmental conditions.