Miracle-dz/UniFAR-FTUs huggingface dataset:
MANIFESTATIONS OF CHRONIC FEED MYCOTOXICOSIS IN LABORATORY RATS UNDER EXPERIMENTAL CONDITIONS

Mycotoxicoses are specific human and animal diseases caused by the certain types of mi-crospores fungi that during their life form toxic substances, the mycotoxins. Toxic effects of these diseases are diverse and depend on the dose of toxin, exposure, animal species, age and sex. Lack of data on physiological mechanism of the pathological influence under combined mycotoxicoses significantly reduces the development of new drugs and methods for treatment of animal mycotoxicoses. This paper is our first report on the features of the clinical, biochemical, and pathomorphological signs of associative mycotoxicoses under experimental exposure of laboratory animals to the most common mycotoxins. The aim of the research was studying clinical signs of chronic combined mycotoxicosis and pathoanatomical changes in organs and tissues under experimental mycotoxins in laboratory rats, as well as the characteristics of intoxication symptoms, the effect of mycotoxins on the reproductive function of rats and their progeny, and morphobiochemical blood parameters. Experiments were carried out on 66 white non-linear rats of both sexes with a body weight of 80-120 g (Krasnodar Research Veterinary Institute vivarium, 2018). After 14-day acclimatization (quarantine) the rats matching experimental conditions were divided into two groups, 33 animals each, according to paired analogue criterium. The experimental rats were fed for 21 days with the feed naturally con- taminated by mycotoxins. The control rats ate toxin-free feed. The spore counts in the contaminated feed was 5. 7½10 4 for Fusarium sp. , 1. 2½10 4 for Mucor sp. , and 2. 0½10 4 for Penicillum sp. Mycotoxin concentration in the samples exceeded the maximum permissible level (3.6 mg/kg for zearalenone, 0.2 mg/kg for ochratoxin A, 6.2 mg/kg for fumonisin B 1 ), which was confirmed by bioassay on la- boratory animals (mice). During the experimental period, all animals were clinically monitored for general status, feed consumption, behavior, response to external stimuli, motor activity, skin and fur condition, tactile sensitivity, functions of the digestive and urinary organs, corneal and dermal reflex-es, and dynamics of weight gain. Hematologic blood tests were also performed. At the end of the study, three rats were euthanized in the experimental group and three rats in control group to identi- fy pathologic and anatomical changes. It was determined that toxic feed leads to a 21 % decrease in body weight of spleen. Proliferation and mitosis of muscle cells are found in the uterus. Thus, the combined mycotoxicosis deeply violates the homeostasis of laboratory animals and leads to multiple pathological changes in the and of the body.
The Effects of Aromatherapy on Autonomic Nerve System and Physical Resistance of a Stress

Purpose: The purpose of this study is to determine effects of aromatherapy on the activity and balance of autonomic nervous system and on physical resistance to stress. Methods: This research is a primitive experimental design which attempts to analyze aromatherapy EKG monitoring on real-time basis. Lavender & Chamomile, each 30 drops, were blended with 100ml Sweet Almond oil. Subjects was laid relaxed, and had insides of his wrists and ankles attached with electrodes for EKG. Right after applied with essential oil onto his philtrum and parotid, started on EKG monitoring. Results: The activities of parasympathetic and sympathetic nerve were changed as time elapsed, with significant differences(p=.000). In multiple comparison, the value was significantly different(p
Correlation between Auditory Brainstem Response Characteristics and Hearing Recovery in Sudden Deafness

Received December 2, 2018 Revised March 19, 2019 Accepted March 22, 2019 Address for correspondence SungHee Kim, PhD Department of OtolaryngologyHead and Neck Surgery, Daegu Fatima Hospital, 183 Ayang-ro, Dong-gu, Daegu 41184, Korea Tel +82-53-940-7353 Fax +82-53-940-7417 E-mail sungheekim@fatima.or. kr Background and Objectives Auditory brainstem response (ABR) can be utilized to verify the hearing threshold and determine the existence of retrocochlear pathology in sudden deafness. However, little is known about the significance of ABR characteristics as a prognostic factor in sudden deafness. The purpose of this study is to evaluate which characteristics of ABR is associated with the prognosis of sudden deafness. Subjects and Method We studied patients who were diagnosed with unilateral sudden deafness from January 2017 to May 2018. ABR results of click stimuli at 90 dB nHL were analyzed by the latency of wave I, III, and V. Results A total of 113 patients (55 men and 58 women) participated in the study. Hearing recovery was observed in 94 cases (83.2%). The absolute latency of wave I, III, and V was prolonged in the affected ears when compared with the unaffected ears. Hearing improvement was only correlated to the interaural latency delay of wave I (Pearson’s r=-0.278, p<0.05). Prolonged interaural latency delay was noted from complete toward slight hearing recovery group. When the hearing outcome was evaluated by the interaural latency delay of wave I at 0.2 ms, the result of under 0.2 ms was significantly better than that over 0.2 ms and no visible wave I. Conclusion Prolonged interaural delay of wave I over 0.2 ms and no visible wave I in ABR showed worse hearing outcome in sudden deafness. This finding may provide ABR as a potential prognostic indicator in sudden deafness. Korean J Otorhinolaryngol-Head Neck Surg 2019;62(8):435-41
The ABCB1 3435C > T polymorphism influences docetaxel transportation in ovarian cancer

Objective To investigate the effect of the ATP-binding cassette transporter superfamily B member 1 gene (ABCB1) 3435C > T single nucleotide polymorphism (SNP) on docetaxel transportation in ovarian cancer cells. Methods ES-2 and SKOV3 cells were transfected with an ABCB1 3435C > T recombinant plasmid, and mRNA expression was detected by real-time PCR. The MTT assay was used to detect the toxicity of docetaxel. High-performance liquid chromatography determined the drug concentration in different cell models to evaluate intracellular accumulation, and a transmembrane resistance experiment was used to assess permeability and evaluate the effect of P-gp activity on drug transportation. A tumor-bearing mouse model was established to evaluate the effect of ABCB1 3435C > T on docetaxel resistance. Results P-gp was overexpressed in cells transfected with the ABCB1 3435C > T plasmid, leading to a significant increase in drug resistance to docetaxel. ABCB1 3435C/wild-type transfection significantly promoted the transport of docetaxel mediated by P-gp compared with ABCB1 3435T/mutant transfection. Conclusion P-gp encoded by the ABCB1 variant allele appears to be more efficient at transporting docetaxel compared with the wild-type allele. The ABCB1 3435C > T SNP dramatically affected the efflux ability of P-gp against docetaxel, and may influence P-gp expression and activity.
Effects of Extracorporeal Shock Wave Therapy in The Maxillofacial Surgery Practice – A Systematic Review

This review intends to provide researchers with a brief summary of extracorporeal Shock Wave Therapy (ESWT), and to bring new perspectives by systematic reviewing of the available data on the results of the various effects of ESWT regarding dentistry and the maxillofacial area. Literature search was conducted on December 2017 using the PubmedMedline, Google Scholar, Scopus and Web of Science databases.Articles between 1989 and 2018 were included.Search was performed using the keywords ‘alveolar, dentoalveolar, maxilla, mandibular, oral and sialolthiasis’ words in combination with ‘shock wave or shockwave’. The studies that were decided to include to this systematic review (n: 35) mostly consist of experimental and clinical studies. The current systematic review stated that ESWT has a success rate of up to 50% in the treatment of sialolithiasis. Shock wave therapy has also different dose-dependent effects on each tissue in the intraoral region. Shock wave parameters that will bring optimal biological effect to any treatment indication are yet to be clarified.International Journal of Human and Health Sciences Vol. 03 No. 04 October’19 Page : 186-195
Color stability of Lucirin-photo-activated resin composite after immersion in different staining solutions: a spectrophotometric study

Introduction Exceptional optical properties characterize teeth. As such, an esthetic restorative material should enable the dental professional to imitate the optical properties of natural teeth accurately. IPS Empress Direct was introduced to the market with the claim that it can mimic optically dental tissues with superior clinical performance. Aim To evaluate the ability of IPS Empress Direct to mimic tooth appearance and color and resist staining. Materials and methods Three disc specimens were prepared for each shade of enamel and dentin of Lucirin-based composite, IPS Empress Direct (Ivoclar Vivadent) (A1, A2, A3, B1, and B2) (total of 60). The specimens were submitted to colorimetric evaluation in comparison after immersion in 10 different solutions (coffee, coffee with sugar, coffee with milk, red tea, red tea with sugar, red tea with milk, tomato juice, pomegranate juice, coke, and distilled water as a control group) for 4 weeks using spectrophotometric analysis. Results After plotting the data and conducting linear regression analyses, IPS Empress showed high potential in mimicking the optical properties of natural tooth color according to the CIE color space. Three solutions showed a change in color higher than 3, coffee, coffee with sugar, and red tea. All other solution showed changes in color that are acceptable clinically. Conclusion IPS Empress Direct can satisfactory mimic teeth appearance and color while manifesting high stability of color resisting staining upon clinical aging.
T cells, dendritic cells and the human immune response to viruses and vaccines

The work presented in this thesis investigates human immune responses to measles virus (MV). Identification of potential MV T cell epitopes for use as part of a new generation component vaccine could overcome problems associated with live vaccines in early life. HLA-A2*0201-restricted epitopes, both predicted sequences and previously identified epitopes, were used to stimulate peripheral blood samples from immune adult donors. Responses were investigated using the IFNy ELISpot. This approach failed to identify dominant epitopes. Therefore methods of enhancing presentation were investigated. To screen for immunodominant epitopes, overlapping 15mer peptides from 3 major MV proteins F, H and NP were used, looking for responses to peptide pools and individual peptides. Although some responses were seen this did not identify any dominant epitopes. In vivo boosting of the response was undertaken by vaccinating HLA-A2 individuals with MMR vaccine. PBMC and serum samples were stored from several time points' pre and post vaccination. Cell phenotyping, serology and response to peptides, along with in vitro expansion of antigen specific cells, were used to assess response in vaccinees. In vitro expansion of cells responding to MV proteins was to be achieved using adenoviral vectors containing cDNA coding for individual MV proteins. This might allow expression of antigen in autologous dendritic cells (DC) via the natural route of infection without encountering the cytopathic effect of live MV on DCs. This work, although unsuccessful, led to the observation that adenovirus-infected DCs suppress T cell proliferation, leading to a novel body of work looking at the effects of adenoviral infection on human DCs. In parallel with this work, similar methodology was used to investigate T cell responses to an OMV based meningococcus B vaccine in vaccinated donors. However it transpired that this vaccine did not stimulate an immune response in the majority of donors investigated.
Atomoxetine and citalopram alter brain network organization in Parkinson’s disease

Abstract Parkinson’s disease has multiple detrimental effects on motor and cognitive systems in the brain. In contrast to motor deficits, cognitive impairments in Parkinson’s disease are usually not ameliorated, and can even be worsened, by dopaminergic treatments. Recent evidence has shown potential benefits from restoring other neurotransmitter deficits, including noradrenergic and serotonergic transmission. Here, we study global and regional brain network organization using task-free imaging (also known as resting-state), which minimizes performance confounds and the bias towards predetermined networks. Thirty-three patients with idiopathic Parkinson’s disease were studied three times in a double-blinded, placebo-controlled counter-balanced crossover design, following placebo, 40 mg oral atomoxetine (selective noradrenaline reuptake inhibitor) or 30 mg oral citalopram (selective serotonin reuptake inhibitor). Neuropsychological assessments were performed outside the scanner. Seventy-six controls were scanned without medication to provide normative data for comparison to the patient cohort. Graph theoretical analysis of task-free brain connectivity, with a random 500-node parcellation, was used to measure the effect of disease in placebo-treated state (versus unmedicated controls) and pharmacological intervention (drug versus placebo). Relative to controls, patients on placebo had executive impairments (reduced fluency and inhibitory control), which was reflected in dysfunctional network dynamics in terms of reduced clustering coefficient, hub degree and hub centrality. In patients, atomoxetine improved fluency in proportion to plasma concentration (P = 0.006, r2 = 0.24), and improved response inhibition in proportion to increased hub Eigen centrality (P = 0.044, r2 = 0.14). Citalopram did not improve fluency or inhibitory control, but its influence on network integration and efficiency depended on disease severity: clustering (P = 0.01, r2 = 0.22), modularity (P = 0.043, r2 = 0.14) and path length (P = 0.006, r2 = 0.25) increased in patients with milder forms of Parkinson’s disease, but decreased in patients with more advanced disease (Unified Parkinson’s Disease Rating Scale motor subscale part III > 30). This study supports the use of task-free imaging of brain networks in translational pharmacology of neurodegenerative disorders. We propose that hub connectivity contributes to cognitive performance in Parkinson’s disease, and that noradrenergic treatment strategies can partially restore the neural systems supporting executive function.
Conditioned Contextual Freezing is A Neurobehavioral Biomarker of Axonal Injury Indicated by Reduced Fractional Anisotropy In A Mouse Model of Blast-Induced Mild Traumatic Brain Injury.

Mild Traumatic Brain Injury (TBI) is an important public health problem generated by closed head injury. This study is focused on the impact of blast-induced mild TBI on auditory trace and delay fear conditioning, models of declarative and non-declarative memory, respectively, and the correlation of conditioned freezing and fractional anisotropy, a measure of axonal state. A supersonic helium pressure wave was generated by a shock tube to blast 8 week old male mice on Day 1 for 1.4 msec with an incident pressure of 16 psi, corresponding to a reflected pressure of 56.5 psi at the mouse head. On Day 3, the mice were subjected to auditory trace- or delay- fear conditioning. On Day 4, contextual freezing in the trained context, and pre-cue and cued freezing in a novel context were determined. After cardiac perfusion on Day 5, ex vivo images were obtained with diffusion tensor imaging at 14.1 Tesla. We observed that delay fear conditioning prevented or reversed the decrease in fractional anisotropy in both the medial and lateral corpus callosum suggesting axonal stabilization of potentially behavioral therapeutic significance. Moderately strong and statistically significant Pearson correlations were found between fractional anisotropy and contextual freezing in the medial and lateral corpus callosum of blasted and sham-blasted delay- or trace- fear conditioned mice. Thus, contextual freezing is a neurobehavioral biomarker for axonal injury in mild TBI and is a reliable and high-throughput behavioral assay for the evaluation of potential therapeutics to treat mild TBI.
A comparative study of influence of isoflurane and propofol on IL1, IL6, TNF serum levels after craniotomy for supratentorial brain tumors

Background: The purpose of this randomized, single-center study was to prospectively investigate the impact of anesthetic techniques for craniotomy on the release of cytokines (interleukin (IL)-1, IL-6, tumor necrosis factor factor-alpha (TNF-α)) and to determine whether intravenous anesthesia compared to inhalational anesthesia attenuates the inflammatory response. Methods and Materials: The study enrolled 60 patients undergoing craniotomy, allocated into two equal groups to receive either Isoflurane (n=30) and Propofol (n=30). Non-invasive hemodynamic monitoring was used. Serum levels of IL-1, IL-6 and TNF-α were evaluated before and at the end of surgery and anesthesia. Results: Although there was a significant rise in serum level of inflammatory cytokine but compared with patients anaesthetized with Isoflurane, patients who received Propofol had significant lower levels of IL-1, IL-6 and TNF-a after surgery (p<0.05). Conclusion: Patients who received Propofol had lower levels of IL-1, IL-6 and TNF-α after surgery. Our findings should incite future studies to prove a potential medically important anti-inflammatory role of Propofol in neuroanesthesia.
GENETIC ASPECTS OF IDIOPATHIC ESCOLIOSIS - A LITERATURE REVIEW

ABSTRACT The idiopathic scoliosis (IS) is the most common form of spinal deformity. The pathogenesis of IS is still poorly understood. Several studies show evidence that the genetic component is determinant to the development of IS. In this setting, a crescent focus has been placed on the identification of genes, associated genetic polymorphisms, and multiple susceptibility loci. This review highlights the genes and genetic polymorphisms currently studied, identified as influential in the genesis of IS, such as MMP-3, IL-6, type I collagen, and vitamin D and estrogen receptors. We concluded that IS remains a complex disease with a polygenic background and that genetic polymorphisms are intrinsically related to this condition. Level of evidence III; Narrative Review.
Temporomandibular disorders: improving outcomes using a multidisciplinary approach

Abstract Patients with temporomandibular disorders (TMD) can become very complex. This article aims to highlight the importance of the multimodal and multidisciplinary approach in this type of patients to improve clinical outcomes. At present we have innumerable techniques and tools to approach this type of patients from a biopsychosocial model where active and adaptive type treatments are fundamental. There are various health professions that have competence in the treatment of TMD, however, although in the most complex cases should be treated simultaneously, still too many patients receive unique treatments and only from one point of view. This review exposes the treatments available from a clinical-scientific perspective and also emphasizes the importance of working in specialized units with those professionals who have competencies on the different conditions that may occur.
Telerehabilitation with Tablet Computers Replaces Face-to-Face Rehabilitation

Abstract Aims: The objective of this study was to introduce mobile telerehabilitation with tablet computers and to verify its practical effectiveness in improving physical function and health status. Methods: In this study, nine adults aged 76.3 ± 3.3 years old participated in 13 exercise sessions led by a physical therapist each lasting 60 min every 2 weeks for a total of 6 months. A physical therapist performed exercises in front of a digital camera which was connected to a lab computer. In a community center, participants performed the exercises while watching the exercise performance on a screen. Intervention was delivered by video conferencing over high-speed mobile Internet. Results: All participants were satisfied with telerehabilitation. We found that patients experienced significant improvement in physical function following participation. Conclusion: Mobile telerehabilitation provides an alternative option for delivering physical exercise and supervision, especially for those with geographical restrictions and limited local professional resources.
Inversion therapy in patients with pure single level lumbar discogenic disease: A pilot randomized trial

Purpose: Backache and sciatica due to protuberant disc disease is a major cause of lost working days and health expenditure. Surgery is a well-established option in the management flowchart. There is no strong evidence proving that traction for sciatica is effective. We report a pilot prospective randomized controlled trial comparing inversion traction and physiotherapy with standard physiotherapy alone in patients awaiting lumbar disc surgery. This study sought to study the feasibility of a randomized controlled trial on the effect of inversion therapy in patients with single level lumbar discogenic disease, who had been listed for surgery. Methods: This was a single centre prospective randomized controlled trial undertaken at the Regional Neurosciences Centre, Newcastle Upon Tyne, UK. It was a prospective randomized controlled trial where patients awaiting surgery for pure lumbar discogenic disease within the ambit of the prestated inclusion/ exclusion criteria were allocated to either physiotherapy or physiotherapy and intermittent traction with an inversion device. Post-treatment assessment made by blinded observers at 6 weeks for various outcome measures included the Roland Morris Disability Questionnaire (RMDQ) Score, Short Form 36 (SF 36), Oswestry Disability Index (ODI), Visual Analogue Pain Score (VAS), magnetic resonance imaging (MRI) appearance and the need for surgery. Avoidance of surgery was considered a treatment success. Results: Twenty-six patients were enrolled and 24 were randomized [13 to inversion + physiotherapy and 11 to physiotherapy alone (control)]. Surgery was avoided in 10 patients (76.9%) in the inversion group, whereas it was averted in only two patients (22.2%) in the control group. Cancellation of the proposed operation was a clinical decision based on the same criteria by which the patient was listed for surgery initially. There were no significant differences in the RMDQ, SF 36, ODI, VAS or MRI results between the two groups. Conclusion: Intermittent traction with an inversion device resulted in a significant reduction in the need for surgery. A larger multicentre prospective randomized controlled trial is justified in patients with sciatica due to single level lumbar disc protrusions. Implications for rehabilitation: Resolution of impairment and disability due to radiculopathy is the aim of any intervention. Avoidance of surgery meant satisfactory resolution of impairment and disability due to radiculopathy. This happened more often in the inversion group to the extent of reaching statistical significance. The 12-point improvement in disability by the Oswestry Disability Index in the inversion group suggests a role for this intervention in disability reduction. Inversion may form part of the conservative rehabilitation of patients with single level unilateral lumbar disc protrusion alongside other forms of physiotherapy. There is a potential secondary impact in the reduction of rehabilitation following surgery.