Miracle-dz/UniFAR-FTUs huggingface dataset:
Protective effects of melanocortins on short-term changes in a rat model of traumatic brain injury*

Objective:Treatment for traumatic brain injury remains elusive despite compelling evidence from animal models for a variety of therapeutic targets. Melanocortins have established neuroprotective effects against experimental ischemic stroke. We investigated whether melanocortin treatment of traumatic brain injury induces neuroprotection and promotes functional recovery. Design:Randomized experiment. Setting:Research laboratory at a university hospital. Subjects:Male Sprague-Dawley rats (n = 215). Interventions:Experimental rat model of diffuse traumatic brain injury, the impact-acceleration model. Measurement and Main Results:Brain tissue nitrites, phosphorylation level of extracellular signal-regulated kinases, and c-jun N-terminal kinases; and expression of active caspase-3, tumor necrosis factor-&agr;, BAX, and Bcl-2 as well as serum levels of interleukin-6, high mobility group box-1, interleukin-10, and brain histologic damage were evaluated 24 or 48 hrs after the insult. Sensorimotor orientation and limb use were evaluated at day 7 and learning and memory at days 23–30 after injury. Posttraumatic treatment every 12 hrs with the melanocortin analog [Nle4, D-Phe7]-&agr;-melanocyte-stimulating hormone (starting 3 or 6 hrs after injury) inhibited traumatic brain injury-induced upregulation of nitric oxide synthesis, phosphorylation level of extracellular signal-regulated kinases, phosphorylation level of c-jun N-terminal kinases, and active caspase-3; reduced expressions/levels of tumor necrosis factor-&agr;, BAX, interleukin-6, and high mobility group box-1; and increased those of Bcl-2 and interleukin-10. These molecular changes were associated with a reduction in brain tissue damage, as highlighted by histopathological findings and improved functional recovery. Pretreatment with the melanocortin MC4 receptor antagonist HS024 abated the positive effects of [Nle4, D-Phe7]-&agr;-melanocyte-stimulating hormone. Conclusions:Our data indicate that melanocortins protect against traumatic brain injury, in a broad time window and through activation of MC4 receptors, by counteracting the main traumatic brain injury-related mechanisms of damage. These findings could have major clinical implications.
An empirical study of third party APK's URL using scriptable API and fast identifier-specific filter

With rising popularization of Android application, third party of APK market has become attractive target of attackers. There have been many research efforts on analyzing APK malware and malformed advertisement. However, so far, the empirical study of the large number of APKs distributed by third party market has not been discussed in detail. In this paper, we present a framework to inspect URL strings to which third party APK connects using headless browser and fast URL filter. In our system, for collecting APK files, navigation scripting with JavaScript enables more interactive web page crawling in order to fetch the results after dynamic web page loading. Besides, FARIS (fast uniform resource identifier-specific filter) is applied for matching URL strings in APK with black list in AdBlock Plus which is one of the most popular ad blockers. We show a result of empirical study of distribution of destination URLs of third party Android applications. Experimental results show that our system can process with reasonable response time for fetching files with scriptable API and filters URL string with feasible computing time compared with previous string matching method such as regular expression.
Role of the carboxy terminus of polypeptide D1 in the assembly of a functional water-oxidizing manganese cluster in photosystem II of the cyanobacterium Synechocystis sp. PCC 6803: assembly requires a free carboxyl group at C-terminal position 344.

The D1 polypeptide of the photosystem II (PSII) reaction center is synthesized as a precursor polypeptide which is posttranslationally processed at the carboxy terminus. It has been shown in spinach that such processing removes nine amino acids, leaving Ala344 as the C-terminal residue [Takahashi, M. , Shiraishi, T. , & Asada, K. (1988) FEBS Lett. 240, 6-8; Takahashi, Y. , Nakane, H. , Kojima, H. , & Satoh, K. (1990) Plant Cell Physiol. 31, 273-280]. We show here that processing on the carboxy side of Ala344 also occurs in the cyanobacterium Synechocystis 6803, resulting in the removal of 16 amino acids. By constructing a deletion strain of Synechocystis 6803 that lacks the three copies of the psbA gene encoding D1, we have developed a system for generating psbA mutants. Using this system, we have constructed mutants of Synechocystis 6803 that are modified in the region of the C-terminus of the D1 polypeptide. Characterization of these mutants has revealed that (1) processing of the D1 polypeptide is blocked when the residue after the cleavage site is changed from serine to proline (mutant Ser345Pro) with the result that the manganese cluster is unable to assemble correctly; (2) the C-terminal extension of 16 amino acid residues can be deleted with little consequence either for insertion of D1 into the thylakoid membrane or for assembly of D1 into a fully active PSII complex; (3) removal of only one more residue (mutant Ala344stop) results in a loss of assembly of the manganese cluster; and (4) the ability of detergent-solubilized PSII core complexes (lacking the manganese cluster) to bind and oxidize exogenous Mn2+ by the secondary donor, Z+, is largely unaffected in the processing mutants (the Ser345Pro mutant of Synechocystis 6803 and the LF-1 mutant of Scenedesmus obliquus) and the truncation mutant Ala344stop. Our results are consistent with a role for processing in regulating the assembly of the photosynthetic manganese cluster and a role for the free carboxy terminus of the mature D1 polypeptide in the ligation of one or more manganese ions of the cluster.
Essays on Online and Multi-Channel Marketing

Firms increasingly adopt online and multi-channel marketing strategies to reach and persuade consumers. Therefore, designing an effective marketing mix is critical to their success. The aim of my dissertation is to understand the strategy behind firms’ channel choices and assess marketing effectiveness. It consists of three large-scale empirical studies examining several important aspects of online and multi-channel marketing. My first essay focuses on the business-to-business (B2B) interactions involving online platforms, which serve as new channels for traditional merchants to reach consumers and grow business. Using data from the daily deal market, we specify a structural model that examines consumer choices on the demand side and firm strategies on the supply side. In particular, we incorporate merchant heterogeneity and allow prices to be jointly determined by merchants and platforms through negotiation; both of these match the real-world complexity but are challenging
Angiographic Findings in Systemic Necrotizing Vasculitis

Angiographic studies were carried out on 21 patients with systemic necrotizing vasculitis. Four basic arterial anomalies were found: (1) saccular microaneurysms appeared in 62% of the patients (2) arterial thrombosis was seen in 81% of patients; (3) arterial stenosis occurred in 81%; and (4) lumen irregularities occurred in 90%. Alterations in the renal vascular flow were also observed in accordance with changes in the cortical medullary differentiation, heterogeneous nephrogram, and prolonged washout. Two patients showed regression of microaneurysms after immunosuppressive therapy. We found angiography to be a low-risk technique of use for diagnosis and followup studies on vasculitis.
The long polar fimbriae operon and its flanking regions in bovine Escherichia coli O157:H43 and STEC O136:H12 strains.

Long polar fimbriae (Lpf) are intestinal adhesins and important virulence factors of pathogenic Escherichia coli strains. We cloned and sequenced the lpf2-1 operon (lpf2ABCD) and its flanking regions of an intimin- and Shiga toxin-negative atypical E. coli O157:H43 strain of bovine origin and also sequenced the lpf2-1 operon of six additional atypical O157 bovine E. coli strains of various serotypes. Nucleotide sequence comparison of these lpf operons showed sequence conservation as they contained only four polymorphic nucleotide positions. Investigation of these O157 strains as well as 13 E. coli Reference Collection (ECOR) strains carrying the lpf2-1 allele revealed high degree of sequence conservation in the lpf2 flanking regions. The lpf2-1 allele is also present in a bovine Shiga toxin-producing E. coli STEC O136:H12 strain, and in vitro adherence assays revealed that the absence of lpf2-1 in this strain did not affect its host cell-binding properties. Our data indicate that lpf2 loci are highly conserved in E. coli isolates; however, its role in adherence might be masked by other uncharacterized adhesins.
Support needs of caregivers of patients with amyotrophic lateral sclerosis: A qualitative study

Abstract Objective The aim of this study was to explore the support needs of Dutch informal caregivers of patients with amyotrophic lateral sclerosis (ALS). Method Individual semi-structured interviews were conducted with 21 caregivers of ALS patients. Audio-taped interviews were transcribed and data were analyzed thematically. Result A total of four global support needs emerged: “more personal time”, “assistance in applying for resources”, “counseling”, and “peer contact”. Despite their needs, caregivers are reluctant to apply for and accept support. They saw their own needs as secondary to the needs of the patients. Significance of results ALS seems to lead to an intensive caregiving situation with multiple needs emerging in a short period. This study offers targets for the development of supportive interventions. A proactive approach seems essential, acknowledging the importance of the role of the caregivers in the care process at an early stage, informing them about the risk of burden, monitoring their wellbeing, and repeatedly offering support opportunities. Using e-health may help tailor interventions to the caregivers’ support needs.
Regulation of c-Fes Tyrosine Kinase and Biological Activities by N-Terminal Coiled-Coil Oligomerization Domains

ABSTRACT The cytoplasmic protein-tyrosine kinase Fes has been implicated in cytokine signal transduction, hematopoiesis, and embryonic development. Previous work from our laboratory has shown that active Fes exists as a large oligomeric complex in vitro. However, when Fes is expressed in mammalian cells, its kinase activity is tightly repressed. The Fes unique N-terminal sequence has two regions with strong homology to coiled-coil-forming domains often found in oligomeric proteins. Here we show that disruption or deletion of the first coiled-coil domain upregulates Fes tyrosine kinase and transforming activities in Rat-2 fibroblasts and enhances Fes differentiation-inducing activity in myeloid leukemia cells. Conversely, expression of a Fes truncation mutant consisting only of the unique N-terminal domain interfered with Rat-2 fibroblast transformation by an activated Fes mutant, suggesting that oligomerization is essential for Fes activation in vivo. Coexpression with the Fes N-terminal region did not affect the transforming activity of v-Src in Rat-2 cells, arguing against a nonspecific suppressive effect. Taken together, these findings suggest a model in which Fes activation may involve coiled-coil-mediated interconversion of monomeric and oligomeric forms of the kinase. Mutation of the first coiled-coil domain may activate Fes by disturbing intramolecular coiled-coil interaction, allowing for oligomerization via the second coiled-coil domain. Deletion of the second coiled-coil domain blocks fibroblast transformation by an activated form of c-Fes, consistent with this model. These results provide the first evidence for regulation of a nonreceptor protein-tyrosine kinase by coiled-coil domains.
Association studies of the CT repeat polymorphism in the 5' upstream region of the cholecystokinin B receptor gene with panic disorder and schizophrenia in Japanese subjects.

The tetrapeptide of cholecystokinin (CCK), CCK-4, is known to induce panic attacks in human subjects, while CCK-8 is reported to have a therapeutic effect on schizophrenia symptoms. Recently, we have identified a novel microsatellite polymorphism in the 5' upstream region of the CCK gene and shown a significant association between this polymorphism and panic disorder. In this study, we have investigated the CCK-B receptor (CCKBR) gene, which is the main constituent of the CCK receptor in the CNS. Recently, a dinucleotide repeat, (CT)(n), in the 5' regulatory region of the CCKBR gene was reported to be associated with panic disorder in Canadian samples. To evaluate an association of the CT repeat with panic disorder and schizophrenia, we genotyped 71 subjects with panic disorder, 154 schizophrenics and 199 controls. However, no evidence of allelic association was found between the polymorphic repeat of the CCKBR gene and either panic disorder or schizophrenia (P = 0.186 and 0.987, respectively). Together with the negative reports on association analyses using other polymorphisms of the CCKBR gene and Japanese samples, the present results exclude a major genetic contribution of the CCKBR gene to susceptibilities to panic disorder and schizophrenia in Japanese cohorts.
Updating Norman's "Adequate Taxonomy": intelligence and personality dimensions in natural language and in questionnaires.

Research on the dimensions of personality represented in the English language has repeatedly led to the identification of five factors (Norman, 1963). An alternative classification of personality traits, based on analyses of standardized questionnaires, is provided by the NEO (Neuroticism, Extraversion, Openness) model (Costa & McCrae, 1980b). In this study we examined the correspondence between these two systems in order to evaluate their comprehensiveness as models of personality. A sample of 498 men and women, participants in a longitudinal study of aging, completed an instrument containing 80 adjective pairs, which included 40 pairs proposed by Goldberg to measure the five dimensions. Neuroticism and extraversion factors from these items showed substantial correlations with corresponding NEO Inventory scales; however, analyses that included psychometric measures of intelligence suggested that the fifth factor in the Norman structure should be reconceptualized as openness to experience. Convergent correlations above . 50 with spouse ratings on the NEO Inventory that were made three years earlier confirmed these relations across time, instrument, and source of data. We discuss the relations among culture, conscientiousness, openness, and intelligence, and we conclude that mental ability is a separate factor, though related to openness to experience.
Simple screening tests for detection of carbapenemases in clinical isolates of nonfermentative Gram-negative bacteria.

BACKGROUND & OBJECTIVE The production of carbapenemases is an important mechanism responsible for the carbapenem resistance. A simple and inexpensive testing method for screening of carbapenemase producers is essential. A prospective study was undertaken to detect metallo-beta-lactamases (MBLs) and AmpC beta-lactamases in nonfermentative Gram negative bacteria and to evaluate the various methods for detection of carbapenemases and MBLs. METHODS A total of 100 Acinetobacter spp. (78 A. baumannii and 22 A. lwoffii) and 140 Pseudomonas spp. (103 P. aeruginosa and 37 other Pseudomonas spp.) were screened for meropenem resistance by Kirby-Bauer disc diffusion method. Modified Hodge test, EDTA disk synergy (EDS) test and AmpC disk test were used for the detection of carbapenemases, MBLs and AmpC beta-lactamases, respectively. RESULTS Forty six (59.0%) A. baumannii, 7 (31.8%) A. lwoffii, 32 (31.1%) P. aeruginosa and 7 (18.9%) Pseudomonas spp. were resistant to meropenem. Among the 32 meropenem resistant P. aeruginosa, 15 (46.9%) were AmpC beta-lactamase producers, 16 (50.0%) MBL producers by EDS test, but only 9 (28.1%) found positive for carbapenemases by modified Hodge test. Among the 46 meropenem resistant A. baumannii, 31 (67.4%) were AmpC beta-lactamase producers, 3 (6.5%) MBL producers, but only 1 (14.3%) was positive for carbapenemases by modified Hodge test. One P. aeruginosa was positive for carbapenemase by modified Hodge test, but was negative for MBL and AmpC beta-lactamase. INTERPRETATION & CONCLUSION MBL production is an important mechanism of carbapenem resistance among Pseudomonas species but not among Acinetobacter species. EDS is more sensitive for detection of MBLs than modifi ed Hodge test. Both EDTA-meropenem and EDTA-ceftazidime combination must be used to detect all the MBL producers. Carbapenemases other than MBL may also be responsible for carbapenem resistance. AmpC beta-lactamase is also a contributory factor for carbapenem resistance among the isolates in the hospital.
The cytoskeletal association of CD11/CD18 leukocyte integrins in phorbol ester‐activated cells correlates with CD18 phosphorylation

Leukocyte adhesion is a regulated process, which involves CD11/CD18 leukocyte integrins. CD11/CD18 acidity may be regulated intracellularly, and the CD18 polypeptide has previously been shown to become phosphorylated on serine and threonine after phorbol ester activation of T cells. Increased adhesiveness is believed to be mediated by regulating the overall avidity of cellular contact. CD11/CD18 integrins have earlier been reported to interact with several cytoskeletal proteins. We have now studied the involvement of the CD18 phosphorylation in cytoskeletal associations. We have investigated the distribution of phosphorylated CD18 between soluble, cytoskeletal and nuclear fractions of T cell detergent lysates. A significant amount of phosphorylated CD18 polypeptides was observed to fraction along with the cytoskeleton, while the majority of the cell surface CD18 molecules remained in the soluble fraction. Putative candidates for this altered cytoskeletal binding of CD11/CD18 were shown to be talin and filamin, which were observed to bind to CD18 cytoplasmic peptides and co‐precipitate with CD18. The importance of the CD18 cytoplasmic domain in the regulation of the leukocyte adhesion was further strengthened by inhibition of phorbol ester‐induced T cell adhesion with a phosphorylated lipopeptide corresponding to the cytoplasmic portion of the CD18. These results indicate that the induced CD18 phosphorylation and the altered cytoskeletal binding of the phosphorylated integrin complex may contribute to the increased avidity of CD11/CD18‐mediated leukocyte adhesion.
Determining factors in the internationalization of hotel industry

Choosing the best way of operating in foreign markets is one of the most important decisions in business internationalization. The main aim of this study is to ascertain whether the choice of entry mode, made according to the impact of various factors, leads to improved export performance. Certain factors influence export performance in different ways, some of which are firm-related, while others lie beyond the firm’s control. It is, therefore, important to know what these factors are and what impact they have in order to be able to choose the optimal foreign market entry mode, as this decision will have a direct effect on performance.
Involvement of inflammatory mediators in macrophage antitumor activity

This review describes the potential role of macrophages in defense against cancer cells and the regulatory involvement of inflammatory mediators in this role. Interactions between macrophage‐derived cytokines (tumor necrosis factor a, interleukin‐1, IL‐6) and their interrelationships with eicosanoids (mainly the cyclooxygenase product prostaglandin E2 and some lipoxygenase metabolites) represent a network that controls the expression of antitumor activity of macrophages either in a cell‐to‐cell contact system between the effector and the target tumor cell or as cell‐free soluble products. Attention is given to the influence of tumor burden on production of cytokines and eicosanoids by macrophages and to the production of these mediators by tumor cells. Emphasis is placed on the roles of TNF‐α and PGE2 in links between inflammatory and antitumor functions of macrophages. Finally, the perspectives and still existing problems in clinical implications of macrophage‐derived cytokines are discussed in terms of a conceivable macrophage‐directed immunotherapy of cancer.
Protein Kinase G from Pathogenic Mycobacteria Promotes Survival Within Macrophages

Pathogenic mycobacteria resist lysosomal delivery after uptake into macrophages, allowing them to survive intracellularly. We found that the eukaryotic-like serine/threonine protein kinase G from pathogenic mycobacteria was secreted within macrophage phagosomes, inhibiting phagosome-lysosome fusion and mediating intracellular survival of mycobacteria. Inactivation of protein kinase G by gene disruption or chemical inhibition resulted in lysosomal localization and mycobacterial cell death in infected macrophages. Besides identifying a target for the control of mycobacterial infections, these findings suggest that pathogenic mycobacteria have evolved eukaryotic-like signal transduction mechanisms capable of modulating host cell trafficking pathways.
Diagnosis of PEMFC for automotive application

Fuel Cells (FC) are electrochemical energy converters that have higher efficiency and energy density than conventional power sources. Proton Exchange Membrane Fuel Cells (PEMFC) based on low operating temperature; pressure and solid electrolyte are well suited for the automotive application. However, they suffer from low reliability and short lifetime. To improve these two points without increasing the cost, low-cost diagnosis procedures for PEMFCs are needed. This paper focuses on PEMFC fault diagnosis in automotive application, the diagnosis is achieved first through a 3D sensitive modeling of PEMFC operation, and then by training a model based on artificial neural network (ANN) for fault state of health classification. The 3D sensitive model allows determination of temperature and current distribution and was validated experimentally. The 3D validated model can be used for introducing flooding and drying out faults and study the behaviors of the distributions of voltages and currents in three space directions for the purpose of diagnosis of the FC.