Miracle-dz/UniFAR-FTUs huggingface dataset:
The comparison of lipid profiling in mouse brain and liver after starvation and a high-fat diet: A medical systems biology approach

We investigated with LC-MS techniques, measuring approximately 109 lipid compounds, in mouse brain and liver tissue after 48 hours of starvation and a High-Fat Diet if brain and liver lipid composition changed. We measured Cholesterolesters (ChE), Lysophosphatidyl-cholines (LPC), Phosphatidylcholine (PC), Sphingomyelin (SPM) and Triacylglycerols (TG's) for liver tissue while for brain tissue we had an extra lipid compound the Plasmalogens. In addition, dynamics of hepatic steatosis were determined in an in vivo mouse model with localized non-invasive Magnetic Resonance Spectroscopy (1H-MRS) techniques. In the experimental design Male C57bl6 mice (age 8-12 weeks) were exposed to three treatments: A: They were fed a chow Diet for a period of approximately 40 days (Control group); B: They were fed a High-Fat Diet, containing 0.25% cholesterol (Ch) and 24% energy from bovine lard for a period of approximately 40 days, C: Or they were exposed to 48 hours of starvation. For whole brain tissue of these mice groups the LC-MS techniques indicated that the brain was rather invulnerable to Dietary intervention. The (phospho-) lipid-composition of the brain was unchanged in the starvation group but the cholesterol-ester content was significantly increased in the high High-Fat Diet group. These observations suggest that the brain lipid composition is insensitive to starvation but can be affected by a high High-Fat Diet. In contrast, for liver tissue both 24 h starvation and the 40 day High-Fat Diet resulted in exponential hepatic fat accumulation, although their time course (measured with 1H MRS) techniques was distinctly different. Mass spectrometry (LC-MS) demonstrated for liver tissue remarkable differences in lipid profiles between treatments. 1H-MRS proved to be a reliable method for frequent, repetitive determination of hepatic fat in vivo and a noninvasive alternative to biopsy. Moreover, LC-MS and Principal Component Analysis (PCA) demonstrated that in liver tissue different lipid end products are formed as result of Dietary composition Apparently, for liver tissue starvation and a High-Fat Diet result in a process called hepatic steatosis which is regulated under both conditions via different metabolic pathways. In addition, 1H-MRS techniques demonstrated for liver that the relative amount of unsaturated bindings is significantly higher in the High-Fat Diet group (P≤0.001), which can be deducted from the relative intensities of the (CH=CH) elements and their conjugated unsaturated elements (C-CCH2C=C). We conclude, comparing brain vs. liver tissue that both tissues have a totally different metabolic response to both treatments. The brain is insensitive to starvation but can be affected by a High-Fat Diet while in liver tissue both treatments result paradoxically in a hepatic steatosis. However, for the liver, the dynamics and the lipid profiles of this process of this hepatic steatosis under starvation or a High-Fat Diet are totally different. © 2010 Nova Science Publishers, Inc. All rights reserved.
Phylogenetic analysis of ITS2 sequences suggests the taxonomic re‐structuring of Dunaliella viridis (Chlorophyceae, Dunaliellales)

We analyzed the ITS2 primary and secondary structure (including Compensatory Base Changes (CBCs)) of 17 new Dunaliella strains (11 D. viridis, two D. tertiolecta, and four Dunaliella sp.), and compared these with other Dunaliella sequences available from the ITS2 database to circumscribe their taxonomic position. The ITS2 primary and secondary structure analysis positioned the majority of D. viridis strains in four main clades, showing that D. viridis is polyphyletic. The detection of at least one CBC among these clades strongly suggests that they could correspond to different biological species. Unexpectedly, while D. viridis var. euchlora (CCAP19/21) was positioned within the subgenus Dunaliella, D. viridis var. palmelloides (CCAP11/34) was positioned clearly outside this subgenus, suggesting that this taxon may not be properly placed in Dunaliella. Furthermore, the detection of at least three compensatory base changes (CBCs) between D. viridis var. palmelloides (CCAP11/34) and the other strains analyzed, confirm that this strain is a different species. For these reasons we propose re‐naming D. viridis var. palmelloides (CCAP11/34) to incertae sedis, and D. viridis var. euchlora (CCAP19/21) to Dunaliella sp. Therefore, the ITS2 primary and secondary structure data suggest a taxonomic re‐structuring of D. viridis.
The complete chloroplast genome of Hibiscus syriacus using long-read sequencing: Comparative analysis to examine the evolution of the tribe Hibisceae

Hibiscus syriacus, a member of the tribe Hibisceae, is considered an important ornamental and medicinal plant in east Asian countries. Here, we sequenced and assembled the complete chloroplast genome of H. syriacus var. Baekdansim using the PacBio long-read sequencing platform. A quadripartite structure with 161,026 base pairs was obtained, consisting of a pair of inverted repeats (IRA and IRB) with 25,745 base pairs, separated by a large single-copy region of 89,705 base pairs and a short single-copy region of 19,831 base pairs. This chloroplast genome had 79 protein-coding genes, 30 transfer RNA genes, 4 ribosomal RNA genes, and 109 simple sequence repeat regions. Among them, ndhD and rpoC1, containing traces of RNA-editing events associated with adaptive evolution, were identified by analysis of putative RNA-editing sites. Codon usage analysis revealed a preference for A/U-terminated codons. Furthermore, the codon usage pattern had a clustering tendency similar to that of the phylogenetic analysis of the tribe Hibisceae. This study provides clues for understanding the relationships and refining the taxonomy of the tribe Hibisceae.
Genetic variation of microsatellite sequences and its relationship with some productive traits of Arctic foxes

the objective of the study was to determine the polymorphism of microsatellite sequences and its relationship with temperament and mean body weight of arctic foxes. measurement of body dimensions in all animals was accompanied by 3 behavioural tests performed to determine the temperament of every animal. Blood samples were also collected. for all primer sequences designed for amplification of microsatellite loci in dogs, analogous products were obtained for arctic foxes. the present study confirms the possibility of using canine genetic markers for genetic study of related species (such as the arctic fox) and suggests the need for standardization of behavioural tests used to determine the temperament of fur animals, because the results of individual tests (empathy test, catch test, restraint test) are not conclusive as to the character of an animal. our study also revealed the presence of alleles characteristic of different weight classes and groups of animals with different temperaments.
Sostenibilidad social en el sector agroindustrial del estado Trujillo–Venezuela. Un constructo relevante para el desarrollo

This paper was to analyze the social sustainability of the Agroindustry of the Trujillo-Venezuela state. Realism was used as an ontological reference, consistent with the inductive empirical epistemological approach of quantitative studies, with a field design, not experimental and cross. The population was constituted by twentytwo (22) companies, whose administrators were given a semistructured questionnaire with 17 items. The processing of the information was done through descriptive statistics. The results show that these companies have a medium level of social sustainability, influenced by the degree of competitiveness of the five dimensions that make it up.
Caractérisation des cellules souches de glioblastomes : nouvelles approches thérapeutiques

Les glioblastomes (GBMs) sont des tumeurs cerebrales au pronostic defavorable. La resistance aux therapies actuelles et la rechute des GBMs pourraient etre due a l'existence de cellules aux proprietes souches. L'objectif de ma these a ete la caracterisation des cellules souches de GBMs (CSGs) isolees a partir de tumeurs. L'analyse du profil souche et de pluripotence des CSGs a montre qu'elles sont maintenues dans un etat souche par SOX2 et que COL1A1 et IFITM1 peuvent etre des cibles therapeutiques potentielles. L'etude de la radiosensibilite des CSGs a travers l'analyse des courbes de clonogenicite a mis en evidence deux groupes dont un «atypique» pouvant etre compose de sous-populations de cellules aux radiosensibilites differentes qu'il conviendra de caracteriser. L'etude de la reparation a mis en evidence deux autres groupes dont un ayant un fort potentiel de reparation qui exprime plus fortement le gene RAD51 apres irradiations. Le traitement par un inhibiteur specifique de RAD51 ralentirait la capacite de reparation de ces cellules. Malgre cette heterogeneite, l'inhibition de la voie Hedgehog (HH) par un vecteur glucuronyle de la cyclopamine, active par le microenvironnement tumoral, inhibe la proliferation et l'auto-renouvellement des CSGs in vitro et ralentit la croissance tumorale in vivo. La voie HH semble etre une cible therapeutique interessante commune a toutes les CSGs. Neanmoins, il est necessaire de prendre en compte l'heterogeneite dans les populations tumorales pour le developpement de la medecine personnalisee.
Erythromycin and tetracycline resistant lactobacilli in the production of a typical dry sausage from the north of Italy

The scope of this study was to assess the frequency of erythromycin and tetracycline resistant lactobacilli in the production chain (skin, minced meat, and stools of eight swine, the natural casing, and the final product at days 0, 21, 35, and 45 of ripening) of a Protected Designation of Origin dry sausage from the North of Italy and in the end products. We isolated colonies of lactobacilli from selective medium supplemented with erythromycin or tetracycline and the most frequently antibiotic resistant species isolated were Lactobacillus sakei, Lactobacillus curvatus and Lactobacillus plantarum The most frequent resistance genes in process line strains were tet(M) and erm(B) while tet(W) and erm(B) were common in strains isolated from swine stools. This study provides evidence of the presence of tetracycline and, to a lesser extent, erythromycin resistant lactobacilli in fermented dry sausages produced in Northern Italy. Although these antibiotic resistant lactobacilli could serve as reservoir organisms, in our study 80% of salami could be considered as safe even though 20% could represent a border line situation regarding the possibility of transferring antibiotic resistant genes to pathogens.
Pro-inflammatory Cytokine Levels and Cancer-related Fatigue in Breast Cancer Survivors: Effects of an Exercise Adherence Program

Purpose This study aimed to determine the effect of an exercise intervention on subjective cancer-related fatigue (CRF) and pro-inflammatory cytokine levels in breast cancer survivors (BCS). Methods BCS with greater than moderate CRF (≥ 4) were recruited and randomly assigned to experimental or control groups. The experimental group participated in a 12-week exercise adherence program (Better Life after Cancer - Energy, Strength, and Support; BLESS). Interleukin (IL)-6 and tumor necrosis factor-α (TNF-α) levels were determined at 3 time points (M1: baseline, M2: post-intervention, and M4: 6 months after intervention). Subjective fatigue was measured using the Korean version of the revised Piper Fatigue Scale. Results In this analysis of participants with physiological fatigue measures available (19 experimental, 21 control), there were no statistically significant differences in IL-6 (F = 1.157, p = 0.341), TNF-α levels (F = 0.878, p = 0.436), and level of fatigue (F = 2.067, p = 0.118) between the 2 groups at baseline. Fatigue in the experimental group showed statistically significant improvement compared to the control only at M2 (p = 0.022). There was no significant relationship between subjective and physiological fatigue at the 3 measurement points. Conclusion The BLESS intervention improved CRF in BCS immediately at post-intervention, and this study presents clinical feasibility for the management of CRF in BCS in the early survivorship phase who are already experiencing fatigue.
Sequential ultrastructural changes during the in vitro cultivation of Trypanosoma cruzi

El desarrollo de Trypanosoma cruzi es determinado por atributos particulares como cambios de forma, probablemente unidos a variaciones en la densidad poblacional. En este trabajo nos aproximamos al topico mediante el estudio de las transformaciones ultraestructurales de T. cruzi en cultivos axenicos. Los resultados indican que el crecimiento de T. cruzi alcanza el pico maximo de densidad poblacional el dia 19 y que a partir del dia 21, la poblacion disminuye continuamente. Tambien senalan una reduccion continua de la concentracion de glucosa en el medio de cultivo; sin embargo, la glucosa no desaparece completamente. Los resultados, ademas muestran que las caracteristicas ultraestructurales de los epimastigotes de T. cruzi varian en la medida que transcurre el tiempo de cultivo. Los cambios incluyen modificaciones nucleares y mitocondriales, asi como dispersion de los perfiles miotocondriales, compactacion de la heterocromatina nuclear y numeros crecientes de vacuolas autofagicas. Los cambios subcelulares descritos en este trabajo, podrian representar una respuesta adaptativa del T. cruzi inducida por las condiciones del medio de cultivo
Impact of salinity Seed sprout characterization of five faba bean (Vicia faba L) varieties

One of the unique properties of earlier stages of plant growth is germination. germination is morphological expression of various metabolic activities in seeds. Plants exposure to the abiotic stresses for instance salinity that influence physiological processes, anatomy, developmental systems and plant development. Agriculture production under arid and semi-arid zone is low owning to many factors for example accumulation of salts in soils and water, total of land area under salinity is about 953 million ha. The aim of this research was to evaluate the germination characters of five faba bean (Vicia faba L.) varieties [Nubaria (1), Giza (843), Sakha (1), Sakha (3) and, Sakha (4)] were using saline solution containing (0, 1000 and 2000 ppm NaCl), were utilized to examine their water uptake, germination percentage, germination index, relative salt injury rate, radicle length, sprout fresh and dry weight, weight losses and seed volume using saline solution . Results indicated that the higher concentrations of salt have a negative impact on water uptake, germination percentage, radical length, sprout fresh and dry weight, seed volume, and relative salt injury rate. At all salinity concentration, the five varieties showed different degrees of salt tolerance. Sakha (3), (4) and Giza (843) had a better salt tolerance index than the other Nubaria (1) and Sakha (1). Nubaria (1), Giza (843) had the highest percentage and index of germination, while Sakha (1) had the shorter radical length and susceptible to salt injury rate. Moreover, Giza (843) and Sakha (3) genotypes can be considered as tolerant to salt stress compared to the other ones. These genotypes also could be used in the breeding program for enhancing faba bean cultivation in newly reclaimed lands. Data suggest a test of seed germination may be useful to use in developing new lines of faba bean to grow in saline soils.
Light thresholds for colour vision in workers of the ant Myrmica sabuleti (Hymenoptera, Formicidae)

Previous studies suggested that workers of the ant species Myrmica sabuleti have different light thresholds for distinguishing different colours. Here we assess these thresholds and find that the light thresholds required to distinguish colours from grey are lower than those necessary to discriminate between two colours. The two thresholds are somewhat lower for ants trained under low versus high light intensity. In every case, the ants' threshold decreases from red to violet. All these thresholds are lower than those required for perceiving shapes. The visual system of workers of M. sabuleti under very low light intensity may thus consist of discriminating only coloured spots from grey and under slightly higher light intensity, differently coloured elements where the eyes are used in superposition mode. Under high light intensity, these ants see (although not sharply) shapes and lines, using their eyes in apposition mode. Moreover, workers of this species demonstrated their best colour discrimination in seeing the colours yellow and blue under high light intensity, and green and violet under low light intensity. Therefore, these ants' visual system may be adapted to the quantitative and qualitative variations in natural light during the day.
Explorations visuelles complexes et maladie d’Alzheimer prodromale : revue de la littérature et présentation de l’étude LYLO partie II

Introduction : la dysfonction visuospatiale apparait precocement dans la Maladie d’Alzheimer (MA), et certaines etudes ont deja mis en evidence une alteration des strategies d’exploration visuelle dans la MA. L’etude LYLO s’est interessee a l’analyse de ces explorations visuelles complexes des le stade Maladie d’Alzheimer Prodromale (MAP), afin de preciser leur interet en tant que biomarqueur diagnostique. Materiel et methodes : etude transversale multicentrique, controlee, analysant des tâches d’explorations visuelles complexes realisees en eye-tracking chez des patients MAP, en comparaison a des temoins sains apparies en âge, et a des patients MA moderee. Resultats : vingt-sept sujets controles (MMSE moyen 28,8), 29 patients MAP (MMSE moyen 26,4) et 23 patients MA (MMSE moyen 23,3) ont ete inclus. Aucune difference significative n’a ete mise en evidence sur nos tâches visuelles, entre sujets MAP et temoins, ni entre sujets MAP et patients MA. Discussion : aucune difference significative n’a ete mise en evidence concernant les sujets MAP. Plusieurs limites de cette etude peuvent expliquer cette absence de resultat significatif (faible puissance, biais de recrutement et de mesure), et nous ne pouvons donc pas eliminer formellement une dysfonction visuospatiale chez les sujets MAP, celle-ci etant de plus en plus rapportee dans la litterature. Necessite de poursuivre les etudes afin de trouver de meilleurs biomarqueurs diagnostiques et pronostiques de la MA, permettant de definir une population cible precoce, dans le but de developper enfin des therapeutiques efficaces luttant contre ce probleme de sante publique.
Proposed Framework for the Factors and Determinants of the Identification of Targeted Customers for Small and Medium Enterprises, Applying to Hotel and Restaurant Services

In the business world, hotels and restaurants are emerging as Small and Medium-sized Projects (SMEs) which are supported by increasing target customers and advertising activities. The entrepreneurs for these projects must have the ability to determine the main methods for increasing their target customers to achieve high profitability. The aim of this research is to specify the contributions of determinations for identifying target customers in hotels and restaurants. The researcher seeks to point out and call attention to the problems of these enterprises and to develop some solutions related to the recent problems, to specify what kind of marketing methods are used and to analyse targeting strategies for enhancing their activity as hospitality enterprises. The study depends on respondents from the entrepreneurs and managers of 10 hotels and restaurants; it uses survey questions to analyse their attitude towards the strategies for identifying target customers. The results of the study indicate some effective methods for identifying target customers. “Customization in administration” takes the primary spot (48%), “fulfilment in past visits” positions number two (46%). Consecutively, “high calibre of administration” (44%), “uncommon consideration regarding visitors” (38%) take part in the rundown.
The New Model of Carcinogenesis: The Cancer Stem Cell Hypothesis

Cancer has long been seen as a disease that arises from mutations and epigenetic alterations that impair the capacity of any cell within the organism to deregulation its proliferation, avoid apoptosis and metastasis, giving rise to malignant transformation. Progressively, these early cancer cells lead to different generations of daughter cells that accumulate additional mutations, acting in concert to drive the full neoplastic phenotype. Adult stem cells are found in numerous tissues of the body and play an important role in tissue development, replacement and repair. In addition, these cells show long-term replicative potential, together with the capacities of self-renewal and multi-lineage differentiation. These stem cell properties are tightly regulated in normal development, therefore their alteration may be a critical issue for tumorigenesis. The Cancer Stem Cell (CSC) theory arises from the poor probability of short-lived differentiated progenitors or terminally differentiated cells to accumulate mutations that become cancer. On the other side, the normal tissue stem cells or early progenitors that already possess the most important characteristics of cancer are an easy target for the accumulation of genetic aberrations which lead to cancer formation. The new defining model for carcinogenesis, the "cancer stem cell theory" was put forward since 1997, when John Dick and coworkers started a series of pioneering investigations to understand whether the functional hierarchy observed in normal hematopoiesis was conserved in blood tumors (Bonnet & Dick, 1997). According to this model cancer is a stem cell disease that places malignant stem cells at the centre of its tumorigenic activity. Cancer stem cells give rise to undifferentiated cells and terminally differentiated cells, as happens in normal tissue renewal, but the major difference between cancer growth and normal tissue renewal is that whereas normal transit amplifying cells usually differentiate and die, at various levels of differentiation, the cancer transitamplifying cells fail to differentiate normally and instead accumulate (ie, they undergo maturation arrest), resulting in cancer growth.
Social Media Influence on Word of Mouth Mediated by Brand Awareness Online Retails

This research aims to : 1 . analyse social media that directly affects word of mouth, 2 . Social media influence word of mouth mediated by brand awareness . The data analysis technique uses Structural Equational Model ( SEM ) with PLS 3 . 0 . The results of this research prove 1 . Social media has influenced on word of mouth, 2 Social media has influenced on word of mouth mediated by brand awareness . The novelty of this research is that brand awareness can increase word of mouth . The findings of this study may contribute to consumer behavior models, at online retails and Tripple duties .