Miracle-dz/UniFAR-FTUs huggingface dataset:
The Arabidopsis B-BOX Protein BBX25 Interacts with HY5, Negatively Regulating BBX22 Expression to Suppress Seedling Photomorphogenesis[C][W]

The B-box domains of BBX25 physically interact with the bZIP domain of HY5, and BBX24 and BBX25 inhibit the activation of BBX22 expression by HY5, probably by forming inactive heterodimers. In contrast with their role during deetiolation, BBX24 and BBX25 can switch roles and function independently of HY5 in the hypocotyl shade avoidance response. ELONGATED HYPOCOTYL5 (HY5) is a basic domain/leucine zipper (bZIP) transcription factor, central for the regulation of seedling photomorphogenesis. Here, we identified a B-BOX (BBX)–containing protein, BBX25/SALT TOLERANCE HOMOLOG, as an interacting partner of HY5, which has been previously found to physically interact with CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). BBX25 physically interacts with HY5 both in vitro and in vivo. By physiological and genetic approaches, we showed that BBX25 is a negative regulator of seedling photomorphogenesis. BBX25 and its homolog BBX24 regulate deetiolation processes and hypocotyl shade avoidance response in an additive manner. Moreover, genetic relationships of bbx25 and bbx24 with hy5 and cop1 revealed that BBX25 and BBX24 additively enhance COP1 and suppress HY5 functions. BBX25 accumulates in a light-dependent manner and undergoes COP1-mediated degradation in dark and light conditions. Furthermore, a protoplast cotransfection assay showed that BBX24 and BBX25 repress BBX22 expression by interfering with HY5 transcriptional activity. As HY5 binds to the BBX22 promoter and promotes its expression, our results identify a direct mechanism through which the expression of BBX22 is regulated. We suggest that BBX25 and BBX24 function as transcriptional corepressors, probably by forming inactive heterodimers with HY5, downregulating BBX22 expression for the fine-tuning of light-mediated seedling development.
A list of the most popular smoking cessation web sites and a comparison of their quality.

To identify the most popular smoking cessation Web sites and to compare their quality, we conducted an Internet survey of 706 U.S. current and former smokers who had already visited such a Web site. Participants cited 133 different sites and rated their quality. Surprisingly, two of the three most frequently visited Web sites were owned by tobacco companies: Philipmorrisusa.com and its information resource Quit Assist (cited by 15.9% of participants) and the R. J. Reynolds site (5.9%). However, these two sites were not perceived as helpful. The other most frequently visited sites were QuitNet.com (10.1% of all participants), the site of the American Lung Association (5.2%), and Webmd.com (4.4%). All the nine most frequently visited sites, which together attracted 56% of all visitors, ranked at or below average for quality or helpfulness, and together, the three sites with the highest quality ratings received only 7% of all visitors. Only one-third of participants received follow-up e-mails. Thirteen of the 18 most popular Web sites were for-profit, and several sites promoted unproven methods. Two sites ranked above average for quality, and both were nonprofit: Smokefree.gov and Anti-smoking.org. Quitsmoking.About. com ranked above average for helpfulness. Direct competition radically distinguishes Web sites from other smoking cessation services. However, competition resulted in the selection of tobacco companies sites and commercial sites rather than sites of the best quality. In general, users were not very satisfied with smoking cessation Web sites, and they had difficulty finding the best Web sites. Resources were scattered over dozens of Web sites of often mediocre quality that duplicated each other.
Voice hearing over time: A qualitative study of a woman without a psychiatric diagnosis

Voice hearing, a complex perceptual phenomenon, is reportedly common in the general population yet understudied in nursing. Nurses meet voice hearers from diverse cultures; their understandings influence assessment. The aim was to explore voice hearing in everyday life in a non-patient population, revealing insights for assessing voice hearing. Method: Interview data was transcribed, translated into English and analyzed by qualitative content analysis. Outcome measures are thematic descriptions. Results: Voice hearing over time involved past (about year 2000) and recent (2009) time periods. Four main themes were: “Transitioning from not-being-at home when home alone to being at-home”, “Shifting overtime from frequent, repetitive voice hearing to inferquent intermittent voice hearing overtime”, “Changing from being disempowered to being empowered” and “Progressing from absence of significant others to presence of significant others”. Study Limitations: Interviewing a single participant residing in Scandinavia produced in-depth descriptions consistent with the study’s aim. The participant had no known psychiatric diagnosis and had not received mental health care when recruited and interviewed. However, we cannot be certain that her voice hearing was or was not non-pathological which raises issues concerning truth value of the study. Conclusions: The results reflect life world existential themes of lived time, lived space, lived body and lived human relation, and offers a framework for guiding and challenging nurses’ assessments of voice hearing. Voice hearing can be understood as a bereavement-grief response. Further research is needed in western and non-western cultures and non-patient populations.
Silencing of Long Noncoding RNA MALAT1 by miR-101 and miR-217 Inhibits Proliferation, Migration, and Invasion of Esophageal Squamous Cell Carcinoma Cells*

Background: MALAT1, a highly conserved long non-coding RNA (lncRNA), acts as oncogene in multiple human cancers. Results: miR-101 and miR-217 can silence MALAT1 expression and then inhibit esophageal cancer proliferation, migration and invasion. Conclusion: Tumor suppressor miR-101 and miR-217 can negatively regulate MALAT1 expression. Significance: These data provide a new mechanism for MALAT1 regulation. MALAT1, a highly conserved long noncoding RNA, is deregulated in several types of cancers. However, its role in esophageal squamous cell carcinoma (ESCC) and its posttranscriptional regulation remain poorly understood. In this study we provide first evidences that a posttranscriptional regulation mechanism of MALAT1 by miR-101 and miR-217 exists in ESCC cells. This posttranscriptional silencing of MALAT1 could significantly suppress the proliferation of ESCC cells through the arrest of G2/M cell cycle, which may be due to MALAT1-mediated up-regulation of p21 and p27 expression and the inhibition of B-MYB expression. Moreover, we also found the abilities of migration and invasion of ESCC cells were inhibited after overexpression of miR-101, miR-217, or MALAT1 siRNA. This might be attributed to the deregulation of downstream genes of MALAT1, such as MIA2, HNF4G, ROBO1, CCT4, and CTHRC1. A significant negative correlation exists between miR-101 or miR-217 and MALAT1 in 42 pairs of ESCC tissue samples and adjacent normal tissues. Mice xenograft data also support the tumor suppressor role of both miRNAs in ESCCs.
The Mutual Constitution of Persons and Organizations: An Ontological Perspective on Organizational Change

I introduce an ontological perspective, which examines the mutual constitution of persons and organizations, to explain continuous versus episodic organizational change. Informed by a cognitive tradition, prior research on organizational change examines individuals’ epistemology , such as knowledge and skills, and their interaction with organizations. From this perspective, organizations change either continuously or episodically because of the conscious cognitive concepts that decision makers use. Informed by a sociocultural tradition, I examine employees’ ontology and the mutual constitution of person and context within organizations. I use the habitus concept to describe how organizational practices structure and are structured by employees’ taken-for-granted experience of reality’s basic dimensions. Based on a two-year ethnography, I investigate how two Wall Street banks’ distinct work practices caused bankers to experience persons, time, causality, and language (“ontology”) in contrasting ways, reproducing the practices that had generated the ontology. The banks changed either continuously or episodically because bankers’ differential ontologies caused them to utilize resources in different, taken-for-granted ways, thus weaving more or less adaptable organizational fabrics.
Towards a prehistory of primates

Using the behaviour of related primates to provide analogies for early humans has a long tradition in archaeology. But these primates too have a past, and experienced particular contexts for the adoption of tool-using. In this pioneering review, the author explores distinctions among chimpanzees in ecology, diet and innovation, sets a wider agenda for a prehistory of primates and explains how archaeology could serve it.
Small Worlds and Regional Innovation

Small-world networks have attracted much theoretical attention and are widely thought to enhance creativity. Yet empirical studies of their evolution and evidence of their benefits remain scarce. We develop and exploit a novel database on patent coauthorship to investigate the effects of collaboration networks on innovation. Our analysis reveals the existence of regional small-world structures and the emergence and disappearance of giant components in patent collaboration networks. Using statistical models, we test and fail to find evidence that small-world structure (cohesive clusters connected by occasional nonlocal ties) enhances innovative productivity within geographic regions. We do find that both shorter path lengths and larger connected components correlate with increased innovation. We discuss the implications of our findings for future social network research and theory as well as regional innovation policies.
Surgical Repair of Residual Shunt after Transcatheter Closure of Secundum Atrial Septal Defect with an Amplatzer Septal Occluder: a Case Report

Abstract Secundum atrial septal defect is the most common congenital heart defect in adulthood. As an alternative to surgery, the transcatheter occlusion appears to be safe. We herein report a case of a residual shunt after transcatheter closure of ASD of a 66 year old man. Surgical repair was indicated. Real and potential problem with device closure of ASD are discussed.
Autologous Surgical Reconstruction for True Venous Hemodialysis access Aneurysms–-Techniques and Results

Purpose Due to their relatively low complication rates autogenous arteriovenous fistulas (AVFs) are the preferred access for hemodialysis. In long-standing AVF, however, aneurysmatic degeneration of the fistula vein can develop. Several treatment options have been published. We evaluated our experience with autologous surgical reconstruction in patients with AVF aneurysms in order to assess complication and patency rates. Methods Demographics and comorbidities of all patients operated on for true AVF aneurysms in our institution, during a 7-year period, were reviewed. Access function and surgical outcomes were evaluated with pre- and postoperative Doppler ultrasonography. Patient survival, primary and secondary access patency rates are presented. Results From April 2006 through March 2013, 31 patients presented with access-associated upper extremity venous aneurysms. The mean time from AVF creation to aneurysm formation was 4.9±3.6 years. The most frequent indication for surgery was aneurysm-associated stenosis (n=16) followed by high flow (n=9), complete thrombosis (n=3) and uncontrolled bleeding (n=3). All patients had autologous reconstructions: 5 resections with end-to-end anastomosis and 26 aneurysmorrhaphies. Overall patient survival at 1 and 2 years was 89% and 84%, respectively. The primary (secondary) patency rates at 6 mo, 1 and 2 years were 87%, 81% and 81% (96%, 96% and 90%), respectively. Conclusions Autologous surgical reconstruction is feasible in the majority of AVF aneurysms. It preserves fistula function and–-in contrast to graft interposition and endovascular repair–-keeps the advantages of an autogenous access: low complication and high patency rates.
Morphological features of the condensed combustion products of burning system "nanoaluminium-air"

The mechanism of whisker crystals formation process by aluminum aerogels burning was studied adapting for ceramic technology problems solution. The correlation of aluminum aerogels burning characteristics and the final combustion products structure was found. The burning temperature, air pressure and nitrogen concentration are significantly effect on the crystals growth mechanism. A wide variety of the crystal morphology in condensed combustion products was found depended on experimental condition.
Implications of leading crop production practices nd human health on environmental quality a

NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Environmental Management. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Environmental Management, [VOL# 151, (March 15, 2015)] DOI# 10.1016/j.jenvman. 2014.11. 024
Host-cell Response to Herpes Virus Infection in Central and Peripheral Nervous Tissue in Vitro

In an organotypic nerve cell culture system, all cells in both the central and the peripheral nervous system (CNS, PNS) components supported replication of herpes simplex virus types I and 2 (HSV I, HSV 2). In HSV I infection, cellular response was particularly characterized by the formation of small syncytia (which involved neurons) and by the presence of bundles of interwoven fine filaments within the nuclei of infected cells. In HSV 2 infection, groups of parallel tubules characteristically formed in the nuclei of infected cells. All cells in the CNS or PNS succumbed to virus infection, some within 24 h (e.g. oligodendrocytes) and others after 48 h (e.g. neurons), with the exception of astrocytes. Although among the first cells to develop virus nucleocapsids in their nuclei, astrocytes became swollen and filled with increased numbers of bundles of glial filaments within 24 h after infection; by 48 h the actual number of astrocytes was increased by as much as threeto fourfold over the number in controls. The results suggest that astrocytes may have a unique mechanism which modifies virus infection and the cells not only survive, but can also become reactive.
Evidence-based practice: issues, paradigms, and future pathways.

Evidence-based practice (EBP) has become a real buzz word, not only in the discipline of nursing, but in all healthcare professions. EBP has been identified as the foundation of accountable, professional nursing practice and it would seem that few could argue with the apparent benefits; however, debate does exist in the literature about whether EBP can realistically be attained. As such, a critical discourse regarding the future of EBP for nursing needs to occur. One of the key questions to be addressed through this discourse is, "what counts as evidence? " A review of the nursing literature on the concept of EBP will be presented in this paper along with a discussion of several of the issues associated with EBP within the discipline of nursing. I will also present some ideas about the implications of the EBP movement in nursing and examine the future pathways for nursing.
Evaluation of PCR, nested PCR, and fluorescent antibodies for detection of Giardia and Cryptosporidium species in wastewater

Giardiasis and cryptosporidiosis are diseases caused by the protozoan parasites Giardia lamblia and Cryptosporidium parvum. Waterborne transmission of these organisms has become more prevalent in recent years, and regulatory agencies are urging that source and finished water be screened for these organisms. A major problem associated with testing for these organisms is the lack of reliable methodologies and baseline information on the prevalence of these parasites in various water sources. Our study addressed both of these issues. We evaluated the presence and reduction of Giardia cysts and Cryptosporidium oocysts in sewage effluent by a combination of indirect fluorescent antibody (IFA) staining and PCR. Our results indicated a 3-log reduction of Giardia cysts and a 2-log reduction of Cryptosporidium oocysts through the sewage treatment process as determined by IFA. We developed a nested PCR to detect Cryptosporidium oocysts and used a double PCR to detect Giardia cysts. A 100% correlation was noted between IFA and PCR detection of Giardia cysts while correlation for Cryptosporidium oocysts was slightly less. On the basis of these results, PCR may be a useful tool in the environmental analysis of water samples for Giardia and Cryptosporidium organisms.
Understanding structure–function relationships of the human neuronal acetylcholine receptor: insights from the first crystal structures of neuronal subunits

Nicotinic ACh receptors (nAChRs) are the best studied members of the superfamily of pentameric ligand‐gated ion channels (pLGICs). Neuronal nAChRs regulate neuronal excitability and neurotransmitter release in the nervous system and form either homo‐ or hetero‐pentameric complexes with various combinations of the 11 neuronal nAChR subunits (α2–7, α9, α10 and β2–4) known to exist in humans. In addition to their wide distribution in the nervous system, neuronal nAChRs have been also found in immune cells and many peripheral tissues. These nAChRs are important drug targets for neurological and neuropsychiatric diseases (e.g. Alzheimer's, schizophrenia) and substance addiction (e.g. nicotine), as well as in a variety of diseases such as chronic pain, auditory disorders and some cancers. To decipher the functional mechanisms of human nAChRs and develop efficient and specific therapeutic drugs, elucidation of their high‐resolution structures is needed. Recent studies, including the X‐ray crystal structures of the near‐intact α4β2 nAChR and of the ligand‐binding domains of the α9 and α2 subunits, have advanced our knowledge on the detailed structure of the ligand‐binding sites formed between the same and different subunits and revealed many other functionally important interactions. The aim of this review is to highlight some of the structural and functional findings of these studies and to compare them with recent breakthrough findings on other pLGIC members and earlier data from their homologous ACh‐binding proteins.
Study of Folded Reflector Multibeam Antenna With Dielectric Rods as Primary Source

Based on a folded reflectarray antenna, a three-beam antenna is investigated using dielectric rods as primary feed placed in open-ended waveguides for obtaining an optimal illumination on the reflector. The performances for a dielectric rod and a combination of three rods are investigated using Ansoft HFSS software. In a first step, dielectric rods have been manufactured and measured at 77 GHz. In a second step, they are used as primary feed of the reflectarray. The corresponding shift of the phase center caused by the rods has been estimated in simulation and accounted for in the antenna arrangement. The radiation pattern measurements show a gain improvement of 2 dB.
Successful use of the frontrunner catheter in the treatment of in‐stent coronary chronic total occlusions

Percutaneous intervention of coronary chronic total occlusion (CTO) from in‐stent restenosis (ISR) is hindered by difficulty to cross the lesion with conventional guidewires. This study describes the successful treatment of five cases of CTOs from ISR using the Frontrunner controlled blunt microdissection catheter. Four of the five cases had failed previous attempts of percutaneous intervention with conventional guidewires. The Frontrunner catheter successfully facilitated the placement of guidewire into the distal true lumen in all five cases. The median crossing time was 10 min. No subintimal delivery of guidewire or vascular perforation was observed. TIMI 3 flow was achieved in all target vessels after further balloon angioplasty or stenting. The Frontrunner device is a safe and effective tool in the treatment of CTOs from ISR. Catheter Cardiovasc Interv 2004;63:462–468. © 2004 Wiley‐Liss, Inc.
An Efficient Algorithm for Dynamic Traffic Equilibrium Assignment with Queues

This paper presents an efficient algorithm for solving the dynamic user equilibrium (DUE) traffic assignment with a one-to-many origin-destination (OD) pattern. To achieve the efficiency of the algorithm, we employ the following three strategies. First, we exploit the decomposition property of the DUE assignment with respect to the departure time from an origin; we consider the algorithm that solves each of the decomposed DUE assignments sequentially. Second, we represent the decomposed DUE assignment by an arc-node formulation, not by using path variables. Third, we take advantage of the fact that the decomposed DUE assignment reduces to (finite dimensional) nonlinear complementarity problems (NCPs); we develop the algorithm based on the globally convergent Newton's method for general NCPs. These strategies, together with graph theoretic devices, enable us to design a new algorithm which does not require path enumeration and is capable of dealing with very large-scale networks. Numerical experiments disclose that the proposed algorithm solves the DUE assignment very rapidly, even in large-scale networks with some thousands of links and nodes where conventional heuristic algorithms do not converge to the accurate equilibrium solution.