Miracle-dz/UniFAR-FTUs huggingface dataset:
OTT_A_234935 1289..1301

Department of PICU, First People’s Hospital of Shangqiu City, Shangqiu, Henan Province, People’s Republic of China Background: Acute myeloid leukemia (AML) is a serious threat to human health. Long non-coding RNA (lncRNA) Taurine-Upregulated Gene1 (TUG1) has been reported to participate in the development and progression of several cancers, including AML. Herein, we aimed to investigate the pathognomonic role of TUG1 in AML cells and its potential mechanistic pathway. Methods: Quantitative real-time PCR (qRT-PCR) assay was applied to detect the expression levels of lncRNA TUG1, miR-193a-5p and Rab10 in AML bone marrow and cell lines. The CCK-8 assay was conducted to assess the cell viability of AML HL-60 and NB4 cells and cell apoptotic assay was performed to assess the cell death. Dual-luciferase reporter assay was carried out to clarify the relationships among TUG1, miR-193a-5p and Rab10. Also, the protein level of Rab10 was examined by Western blot assay. Results: LncRNA TUG1 was up-regulated in AML bone marrow and cells. Functional analysis showed that the silencing of TUG1 suppressed cell viability, while promoted cell death in AML HL-60 and NB4 cells. TUG1 targeted miR-193a-5p and negatively regulated miR-193a-5p expression. Overexpressed miR-193a-5p resulted in the decrease of cell viability and the increase in the cell death in AML cells. Restoration experiments proved that TUG1 regulated the cell viability and death of AML cells through regulating the miR-193a5p/Rab10 axis. Rab10 was a direct target of miR-193a-5p and was inversely regulated by miR-193a-5p. TUG1 regulated the cell viability and death of AML cells through upregulating Rab10. Conclusion: Silencing of lncRNA TUG1 induces a cytotoxic effect on AML cell lines through sponging miR-193a-5p and the suppression of Rab10.
Simulation-Based Engineering of Humanized Scfv Antibody against hTNF-α

Background: Uncontrolled activity of tumor necrosis factor alpha (TNF-α) as pro-inflammatory cytokine has been linked with pathogenesis of autoimmune/inflammatory diseases. Therefore, modulating of TNF-α associated biological pathways is a promising strategy for alleviating of such diseases. In view of this, the use of antibody fragments such as single-chain variable fragments (scFv) in therapeutic applications has been gained much attention in terms of pharmacokinetic as well as production and therapeutic costs. Methods: In the current investigation, the previously designed and humanized hD2 antibody was modeled and docked onto the TNF-α structure. The binding free energy was predicted for the complex of hD2-TNF-α using molecular dynamics calculation followed by per-residue energy decomposition for residues of hD2. In addition in silico mutations of important amino acids at the binding site of enzyme were performed and the binding free energy was calculated for mutant forms of scFv in complex with TNF-α. Results: The analyses of the results proposed Y27F mutation in heavy chain CDR1 of hD2 scFv antibody may be considered as a promising substitution. Conclusion: The results may be used for designing new anti-TNF-α antibody with improved activity.
Synthesis of liponucleotides using bacterial phospholipase D

A series of conjugates of pharmacologically promising modified nucleosides with phospholipid was synthesized. Some liponucleotides were originally produced. Soybean lecithin served as the donor of phosphatidyl residue. Phospholipase D from strain Streptomyces netropsis BIM B-428D was engaged as biocatalyst in transphosphatidylation reaction. The yield of liponucleotide synthesis reaction varied in the range 44–95 mol% depending on the acceptor of phosphatidyl residue. 65 mg (about 68 µmoles) of pure phosphatidylclofarabine was recovered by chromatography on silica gel column, resulting in 68 mol% yield calculated from the amount of modified nucleoside supplied into the reaction mixture.
Phylogenetic Analysis of Human Bocavirus Isolated from Children with Lower Respiratory Tract Infections in Baghdad, Iraq

Acute respiratory infection is the major cause of morbidity and mortality worldwide, several types of viruses associated with this disease. This study aims to determine the infection rate of human bocavirus in children with lower respiratory tract infection and identify the genotyping among study population. Cross sectional study which consists of 122 children under five years old suffering from lower respiratory tract infection (75 males and 47 females); their aged from range 1 month to 60 months. They were attending respiratory wards in Fatima Al-Zahraa Hospital, Al-Elwiya Pediatrics Hospital, Ibn Al-Baladi Hospital and Pediatrics Protection Hospital in Baghdad, during the period from December 2017 to February 2018. Nasopharyngeal samples were collected from each participant, then used for DNA extraction and amplification with specific primers. Out of all 122 samples, eight samples were positive for HBoV (6.6%). Most infection were recorded in males 5(62.5%) and patients in age group 1-30 months 7(87.5%). The results of phylogenetic analysis for HBoV DNA isolated from nasopharyngeal swabs revealed all local isolates (8 isolates) are with HBoV type 1, half of the local isolates (4) were closed to Iranian isolation and the other four were closed to isolates from different regions (Tunisia, Italy and Argentina isolation). Some variant in amino acid noticed after alignment to the local isolates with some Gene Bank isolates and deletion of amino acid occur in isolates number one and five in more than two position but the substitution revealed in isolate number six in three position. In conclusion, infection rate of HBoV-1 comparable with result of neighboring countries, local isolate of HBoV-1 have their characteristic genetic structure which differentiation them from other international isolate.
The Economics and Ethics of Farm Animal Welfare-The Case of Chick Culling and its Alternatives

Purpose – Investigating consumer preferences for boxes of eggs which are produced without the culling of male layer-type chicks in layer hen production. Furthermore, investigating consumer preferences for labels from different certifying bodies. Design – An online survey including a discrete choice experiment was conducted among 526 German consumers in 2018 and early 2019. Mixed logit models in preference and willingness to pay (WTP) space were estimated. Findings – The preferred alternative is gender determination of incubated eggs, but also dual-use poultry with free-range rearing of cockerels was approved. Labels from public authorities and the animal protection organisation were highly approved. By contrast, retailers were not considered suitable for the certification of production claims. Research limitations – A hypothetical setting was employed. A revealed preferences approach is suggested for future research. Practical implications – Evidence for a wide approval of in ovo gender determination was found. Free-range rearing of cockerels was the most promising for the marketing of dual-use poultry products. Furthermore, public authorities and well-established third parties should engage in the field of animal welfare labeling. Originality/value – This is the first study focusing on preferences and WTP for alternatives to chick culling which will soon be available on the market, and for institutions certifying production claims. Findings help political decision makers, when looking for alternatives to the culling of day-old chicks, which are considered more acceptable by the public.
Checkmate 032: Nivolumab (N) alone or in combination with ipilimumab (I) for the treatment of recurrent small cell lung cancer (SCLC).

100Background: Patients (pts) with advanced (adv) SCLC after first-line platinum-based chemotherapy have limited options. Nivolumab, a programmed death-1 (PD-1) immune checkpoint inhibitor, is approved for previously treated metastatic NSCLC in the US and for squamous NSCLC in the EU. Nivolumab + ipilimumab, a cytotoxic T-lymphocyte antigen-4 immune checkpoint inhibitor, has shown durable responses in multiple tumor types. CheckMate 032 was designed to evaluate nivolumab +/- ipilimumab in adv tumors including SCLC. Methods: AdvSCLC pts with progressive disease (PD) after ≥1 platinum-based chemotherapy, regardless of platinum sensitivity or tumor PD-1 ligand 1 (PD-L1) expression, were eligible. Pts received nivolumab ([mg/kg] N3 Q2W) or nivolumab + ipilimumab combination (N1 + I3 or N3 + I1 Q3W for 4 cycles then N3 Q2W). Primary endpoint was objective response rate (ORR). Additional endpoints were safety, overall survival (OS), progression-free survival (PFS), and biomarkers. Results: 180 pts were enrolled.
Etude descriptive des patients ayant présenté une perforation myocardique après l’implantation de matériel de stimulation cardiaque : Evaluation des facteurs de risque, de la prise en charge diagnostique et thérapeutique et réalisation d’un algorithme décisionnel

Contexte : A ce jour, les perforations myocardiques compliquant l’implantation d’un stimulateur ou d’un defibrillateur sont peu documentees dans la litterature, malgre un enjeu de sante publique certain (diminution de la morbi-mortalite des patients et des couts engendres). Cette analyse decoule de notre experience, dramatique et bordelaise, de 2 perforations post-implantation de MICRA™. Objectifs : Suite a l’evaluation de nos pratiques, renforcee par l’etude de la litterature, nous avons souhaite realiser un algorithme diagnostique et therapeutique afin d’aider les praticiens dans la prise en charge des perforations. Methodes : Nous avons inclus de maniere retrospective et consecutive tous les patients traites pour une perforation cardiaque. La presentation clinique et paraclinique, ainsi que la prise en charge immediate et a court terme ont ete analysees. Resultats : De janvier 2012 a juin 2016, 48 perforations ont pu etre etudiees, dont 42 successives a une des 3234 procedures realisees dans notre centre. Soixante-quatorze pour cent des femmes etaient âgees de plus de 75 ans, 3 patients avaient un antecedent de radiotherapie, 56% etaient traites par un antiagregant plaquettaire et 34 % par un anticoagulant. Dix scanners thoraciques ont ete realises dans notre cohorte. On observe une difference significative entre l’impedance et le seuil de stimulation avant et apres perforation. (Respectivement 556,78 ohms +/- 141,79 versus 485,82 ohms +/-101,48, p = 0,0019 et 0,96 V +/- 1,25 versus 1,62 V +/- 1,7, p=0,047). Quarante pour cent des patients n’ont beneficie d’aucune therapeutique alors que, dans le reste de la cohorte, un traitement percutane a ete privilegie. Conclusions : Nous retrouvons comme facteurs predictifs les femmes âgees, les patients traites par corticotherapie ou radiotherapie. L’anticoagulation et surtout l’anti-agregation plaquettaire peuvent majorer un epanchement pericardique, pouvant devenir alors symptomatique. Le scanner cardiaque est a ce jour le gold standard pour le diagnostic. L’extraction percutanee et l’abstention therapeutique decidees dans un centre chirurgical apparaissent comme les therapeutiques de reference hormis dans les cas des atteintes extracardiaques.
Employee’s Involvement Culture of Star Hotels in Pokhara

Since the star hotels are directly relates to the both internal and external tourism it has important contributory role to the nation therefore smooth operation of the hotels is most. Looking to current performance of the hotels some of the hotels are in loss condition and therefore it becomes challenging issues for both hotel management and Nepal government. Several numbers of literatures have explained an employee involvement practice is one that affects organizational performance. Therefore this study attempts to explore existing situation of employee involvement culture and its association with performance of star hotels in Pokhara. Since Nepal government announced Nepal Visit Year 2020 this study is highly relevant. This study compare between hotels having involvement practices and non-involvement practices in association with their financial performance in Pokhara. The advantage of this research is broadly defined into three sets of variables as employee empowerment, team work and carer development that focus interrelationship with star hotel’s performance. The result revealed the hotels which have adopted employee involvement practices mostly get good financial result and vice versa. The result shows there is strong relationship between employee empowerment and team work practice with better financial result. Though previous literature explained career development is strong variable that supports performance, but in the Nepalese context this variable does not have significant impact. Hence, the study concludes that the management must give high priority to employee empowerment and team work and less priority to career development for better financial performance of the star hotels in Pokhara.
Analysis of Helmet Detection Using LENET and Transfer Learning

: Nowadays the most preferred means of transport is two wheelers. The main reason behind it is two wheelers are economical and flexible . This increase in usage of two wheelers has also increased the number of road accidents. This is due to traffic rule violations like over speed, not wearing helmet, drunken driving etc. Implementing an effective automatic helmet detection system helps in decreasing two wheeler accidents to an extent. This paper aims to implement a real time helmet detection system in which license plate is extracted if the bike rider is not wearing helmet. This paper also compare two approaches , LeNet and Transfer Learning, used for detecting helmet. Convolution Neural Networks are employed for implementation.
Value measurements: the impending barrier to orthopaedic surgery for obese patients

Obese patients needing total joint arthroplasty (TJA) face inherent elements of discrimination due to exposure, operating time, and perceptions of higher complication rates. An expanding literature supports that perception, which is now juxtaposed on newly introduced Centers for Medicare and Medicaid Services (CMS) risk adjusted performance measures. If those performance measures are not adequately risk adjusted, obese patients face a higher likelihood of discrimination. The performance measures were reviewed for their quality of risk adjustment and their potential impact. A literature review was performed to help define the risk of an elevated body mass index (BMI). The risk adjustment model for both National Quality Forum (NQF) 1550 (TJA Complications) and NQF 1551 (TJA 30 Day Readmissions) have a C-statistic of 0.65. Combined they represent hospital exposure for repayment of up to 5% of Medicare reimbursement. Although literature supports an association of obesity with increased risk for complications, more recent literature demonstrates that BMI itself might not be a reliable independent predictor of risk if other associated comorbidities are used. The C-statistic for both current CMS total joint performance measures is low. Hospitals face increased jeopardy for penalties because of patients with higher risk if risk adjustment does not protect them for providing their care. It is predictable that they will shed risk by avoiding patients with higher risk, including those with high BMI. This is occurring nationally with the setting of threshold BMI by groups, hospitals, and health care systems despite the evidence that the BMI itself may not be as predictive as perceived.
Video Genre Classification using Convolutional Recurrent Neural Networks

A wide amount of media in the internet is in the form of video files which have different formats and encodings. Easy identification and sorting of videos becomes a mammoth task if done manually. With an ever-increasing demand for video streaming and download, the Video Classification problem is brought into foresight for managing such large and unstructured data over the internet and locally. We present a solution for classifying videos into genres and locality by training a Convolutional Recurrent Neural Network. It involves feature extraction from video files in the form of frames and audio. The Neural Networks makes a suitable prediction. The final output layer will place the video in a certain genre. This problem could be applied to a vast number of applications including but not limited to search optimization, grouping, critic reviews, piracy detection, targeted advertisements, etc. We expect our fully trained model to identify, with acceptable accuracy, any video or video clip over the internet and thus eliminate the cumbersome problem of manual video classification.
WASTE MANAGEMENT OF ENERGY SECTOR WASTE - FLY ASH FROM BURNING CRUDE OIL AS CATALYSTS

Due to the increased world population and demand for electricity, the level of greenhouse gases and pollutions is still growing. During last years’ scientists were trying to provide new technologies to reduce the amount of emitted NOx and utilize the waste from energy sector. One of the most promising methods may be fly ash waste (due to its chemical composition [1]) implicated as catalyst used in Selective Catalyst Reduction, which is based on conversion of nitrogen oxides to elementary nitrogen. The main aim of this study was to check the possibilities of application fly ash from burning crude oil as low-cost and eco-friendly catalyst to be used in that process.
The circumstances and impact of powerlessness in psychiatric nursing.

Nurses comprise the largest professional group in healthcare; yet, they do not possess a degree o f power compatible with their numbers. Nursing, being a predominantly female profession, suffers many o f the same oppressive forces imposed on women in a patriarchal society. In addition, nurses suffer the oppression inherent in the hierarchical healthcare structure. A sense of powerlessness may compromise nurses' competency, and this is o f particular importance in the mental health field where nurses' ability to advocate for patients can significantly impact the care given. The aim o f this study was to explore the situations in which acute care psychiatric nurses feel most powerless, and the impact this has on them, both personally and professionally. A qualitative research design was used, and data were collected through four focus groups with four to five nurses in each group. Three groups were conducted in lower mainland hospitals, and one group took place in an interior British Columbia hospital. Four common situations in which psychiatric nurses feel powerless, emerged from the data. These include (a) those related to medicine's dominance and control; (b) when nurses feel they are in a no-win situation; (c) when there is a lack o f communication and support; and (d) when there are system problems. The personal and professional impact o f feeling powerless is explored. Implications for both nursing and counselling are discussed.
ANTICANCER PROPERTY OF MEDICINAL PLANTS

Cancer is a major public health problem in most of the developed countries in around the world; however, there has been a notable improvement in the survival rate of patients over the past few decades owing to early detection and progress in medical treatment [1][2]. A few numbers of patients with cancer receive chemotherapy or chemo radiotherapy and benefit from treatment with anticancer drugs [1]. However this anticancer drug causes many side effects with a variety of symptoms, such as nausea, vomiting, anorexia, diarrhoea, oral mucositis, and numbness, because of their toxic effects on normal cells/tissues [10]. Although many valuable strategies have been developed to treat or prevent these side effects, as these side effects often compromise patients’ quality of life. Therefore, an alternative or novel approach to treat or prevent these side effects is required[10]. The causes for cancer can be the use of tobacco, alcoholic beverages, infections such as hepatitis B, hepatitis C and HPV. There is a constant demand for new therapies of treatment and prevent this life from this dangerous threatening disease. The plant kingdom produces a lot of naturally occurring secondary metabolites which are being investigated for the anticancer activities for the development of new clinical drugs[10]. Since many years herbal medicines had been used and are still used in developing countries around the world as the primary source of medical treatment. Many plant species in the plant kingdom are already being used to treat or prevent the development of cancer. In recent years, several Kampo medicines (Japanese traditional herbal medicineswere imported from China 1,500 years ago and developed independently)[3] have been investigated using animal models and clinical trials to assess their effects on chemotherapy-induced side effects. In this paper we will discuss about the demand for natural compounds from medicinal plants and their properties which make them targets for potential anticancer treatments.
Brain-Computer-Interface: A Survey on Feature Extraction Techniques for Brain Signal

: This article address to the various feature extraction techniques as Auto Regressive, Band Powers, Cross-Correlation, Fast Fourier Transformations, Hjorth Parameters, Independent Component Analysis, Phase Locking Value, Power Spectral Density, Principal Component Analysis, Time Frequency Representation, Wavelet Packet Decomposition, Wavelet Transformation, and their comparative discussion with their advantages and disadvantages in modern application areas.
Diagnostic Accuracy of the Triglyceride and Glucose Index for Insulin Resistance: A Systematic Review

Introduction. The triglyceride and glucose (TyG) index has been described as a biochemical marker of insulin resistance (IR); however, its diagnostic accuracy remains uncertain. Objective To summarize the evidence assessing the diagnostic accuracy of the TyG index regarding IR. Methods A comprehensive search in MEDLINE, EMBASE, Web of Science, and Scopus was performed without any language restriction. Studies assessing the diagnostic accuracy of the TyG index against the hyperinsulinemic-euglycemic clamp (HIEC) or any other IR biochemical were assessed independently and in duplicate. Diagnostic accuracy measures (sensitivity, specificity, positive predictive value, negative predictive value, and likelihood ratios) were extracted independently and in duplicate. The QUADAS-2 tool was used to assess the risk of bias of independent studies. Results We identified 15 eligible studies with 69,922 participants and an overall quality of low to moderate. The TyG index was evaluated by HIEC and HOMA as reference tests. The highest achieved sensitivity was 96% using HIEC, and the highest specificity was of 99% using HOMA-IR, with a cutoff value of 4.68. AUC values varied from 0.59 to 0.88. Cutoff values for IR were variable between studies, limiting its comparability. Conclusion In this systematic review, we found moderate-to-low quality evidence about the usefulness of the TyG index as a surrogate biochemical marker of IR. Due to the lack of a standardized IR definition and heterogeneity between studies, further validation and standardized cutoff values are needed to be used in clinical practice.