Miracle-dz/UniFAR-FTUs huggingface dataset:
Influence d'un bain de bouche sur la présence de bactéries cariogènes au sein du biofilm dentaire

La carie est le 4eme fleau mondial selon l'Organisation Mondiale de la Sante (OMS). C'est une maladie infectieuse polymicrobienne, transmissible et multifactorielle, detruisant les tissus durs des dents.Le but de notre etude etait d'observer l'influence d'ELUDRIL*, bain de bouche contenant de la chlorhexidine, antiseptique a large spectre d'action, sur la presence des 2 principales bacteries cariogenes du biofilm dentaire : Streptococcus mutans etLactobacillus.Un traitement de 3 semaines par ELUDRIL* (a raison de 2 bains de bouche par jour) a ete prescrit aux 64 patients de l'etude.Nous avons evalue avant et apres ce traitement la quantite de Streptococcus mutans et Lactobacillus salivaires et leur proportion respective par rapport a la charge bacterienne salivaire totale (grâce au test salivaire CARIO-ANALYSE*), le pouvoir tampon salivaire (egalement grâce au test salivaire CARIO-ANALYSE*) et l'indice de plaque (de Silness et Loe). ELUDRIL* s'est avere efficace pour diminuer l'indice de plaque (chez 76% des patients) et ne semble avoir aucune incidence sur le pouvoir tampon salivaire (chez 86% des patients). Cependant, nous avons observe apres le traitement une augmentation des quantites de Streptococcus mutans et de Lactobacillus chez respectivement 41% et 66% des patients et une augmentation des pourcentages de Streptococcus mutans et de Lactobacillus chez respectivement 39% et 59% despatients, ce qui ne va pas dans le sens des resultats de la litterature.L'action d'ELUDRIL* in vivo sur les bacteries cariogenes Streptococcus mutans et Lactobacillus semble donc plus complexe a etudier, car de tres nombreux biais ont pu fausser nos resultats, tels que la mauvaise observance du traitement, l'absencede scellement prealable des reservoirs bacteriens, le non respect des restrictions necessaires a l'interpretation des tests salivaires, l'alimentation et l'hygiene bucco-dentaire pendant le traitement, les limites des tests salivaires, la variabilite destemps de prelevements salivaires et les quelques detartrages realises.Il est donc plus difficile de se prononcer sur les resultats obtenus pour ces 2 especes bacteriennes et on ne peut a priori pas indiquer ELUDRIL* dans la prevention carieuse.
Leadership and culture in occupational safety: its influence on safe work practices

La investigación se basó en estudios descriptivos y correlacionales, utilizando el método de estudio de casos para explorar el comportamiento de los factores predictivos de la cultura de seguridad, agrupados en 10 dimensiones, que hacen énfasis en el liderazgo en seguridad como variable interviniente en correlación con el desempeño en seguridad de los trabajadores, y medir los cambios de los comportamientos seguro de los trabajadores, cuando los supervisores y mandos medios transforman sus conductas y motivan la aparición de un cambio cultural. Se emplearon dos tipos de cuestionarios, para evaluar la cultura y prácticas que caracterizan el liderazgo en seguridad desde la visión y acción de la alta gerencia, los gerentes de nivel medio, supervisores y los profesionales que gestionan la seguridad. La investigación exigió modificar el estado de las dimensiones de la cultura y liderazgo en seguridad, con un análisis de su impacto en la efectividad del Proceso de Gestión de la Seguridad Basada en los Comportamientos.
Structure-function analysis of C2 domains of multiple C2 domains and transmembrane region proteins, located at plasmodesmata

All plant biologists in research history observed the paramount role of cell-to-cell interconnections, so-called plasmodesmata. The plasmodesmata ultra-structure and the elements involved in its regulation are not fully known yet. However, the new viewpoint sees plasmodesmata as specialized membrane contact sites (MCS) – where the endoplasmic reticulum and the plasma membrane get tethered together within the pores – brings us an original hypothesis on the importance of the spoke-like tethering elements. The identity of such elements was not uncovered yet, but recent research has proved plasmodesmata localization of the plant Multiple C2 domains and Transmembrane region Proteins (MCTPs). As members of this family possess all the required features for membrane tethering, they are perfect candidates for being membrane tethers. Indeed, they display a transmembrane domain, that could bind the endoplasmic reticulum membrane, and several C2 domains – known in mammal MCS tethers to bind lipids and/or calcium ions – that could bind the plasma membrane. The objective of our work was to predict the membrane and/or calcium interactions of the C2 domains of two plasmodesmata-located MCTPs: QUIRKY/AtMCTP5, from Arabidopsis thaliana, which is involved in morphogenesis regulation and NbMCTP11, from Nicotiana benthamiana. For this, we had to establish a series of bioinformatics protocols to delimit the C2 domain sequences, make 3D models and, finally, predict their docking to model plant membrane and possible calcium dependence. The results of the modeling simulations suggested interactions with the membrane, as most of the studied domains were able to interact with the lipids polar heads, but also on more specific features such as phosphatidylinositol phosphate binding sites in NbMCTP11 C2D and calcium binding sites in AtMCTP5 C2D domains. In vivo assays on the full length NbMCTP11 proved that the protein localizes at plasmodesmata and truncated mutants showed different phenotypes, revealing different roles of the different parts of the protein. Further analysis has to be carried out on this part but preliminary results showed convergence between mutant localization and domains behavior.
Medida de calidad de la planificación de recogida de residuos a partir del conocimiento del experto utilizando aprendizaje de preferencias

Resumen—La investigación incluida en este trabajo surge a raı́z de la necesidad de optimizar globalmente el proceso de recogida de residuos de la empresa Consorcio para la Gestión de los Residuos Sólidos de Asturias (COGERSA). Hasta ahora, el diseño de la planificación se iba realizando progresivamente bajo demanda, por lo que su optimización no es global. Los expertos crearon la planificación de forma manual construyendo localmente las nuevas rutas de los concejos en cuanto estos solicitaban el servicio de recogida. Un diseño global teniendo en cuenta todas las caracterı́sticas de este proceso excede la capacidad de un experto humano debido, principalmente, a la gran cantidad de recursos humanos y materiales disponibles, al alto número de contenedores que se deben recolectar, a las diferentes limitaciones de tiempo y a la dependencia del trazado de la carretera. Normalmente, este tipo de problemas se resuelve utilizando algoritmos de optimización de planificación, para los cuales uno de los requisitos es diseñar una función objetivo que evalúe la calidad de la planificación. En este sentido, el objetivo de este trabajo es diseñar una función objetivo a partir del conocimiento de los expertos que sea capaz de evaluar la calidad de una planificación global de recogida de residuos, como paso previo a una posterior optimización de la planificación. Nuestra propuesta se enmarca dentro del aprendizaje de preferencias, donde además, se propone simplificar las decisiones de los expertos, ya que deberán decidir no entre dos planificaciones completas de la ruta, sino entre dos pares de rutas, que supone una tarea bastante más asequible, incluso abordable. El resultado será una función de ranking de planificación de rutas, donde dada una planificación candidata, se le asignará una puntuación que la coloque dentro del ranking. Palabras clave—Aprendizaje de preferencias, Caracterización de rutas, Función objetivo
Universidad y Ciudad. Querétaro, ¿Ciudad Universitaria?

El proposito del articulo es identificar las condiciones de Queretaro para constituirse en cluster de servicios educativos en el nivel superior, focalizando el analisis en el proceso de articulacion de factores y actores que inciden en la creacion de conglomerados educativos. A traves del enfoque de ciudades universitarias y del desarrollo territorial se plantea que la educacion superior, vista como un producto, contribuye a que en torno a ella se organicen servicios de salud, vivienda, alimentos, entre otros, que potencializan el desarrollo. Para el estudio de Queretaro como potencial Ciudad Universitaria se describe la oferta de educacion superior por matricula, regimen (publico-privado), tamano de institucion y programas de estudio, areas de conocimiento, y prestigio internacional.
Reproductive cycle of male wall lizard, Podarcis vaucheri (Reptilia: Sauria: Lacertidae), in Djurdjura, Northern Algeria

The reproductive cycle of male wall lizard Podarcis vaucheri (Boulenger, 1905) living in Djurdjura Mountain, was analyzed from April until October 2014. Its seasonal mixed-type cycle was characterized by three phases: sexual activity, quiescence and recrudescence. After the emergence of lizards from hibernation, an intense sexual activity was observed in spring (April, May, and June), at which the testicular weight and seminiferous tubules diameter reached their maximal values. At this period, spermiogenesis was also maximal and the spermiation allowed the release of a large quantity of spermatozoa in both the seminiferous tubules and epididymis lumen. In July, a short sexual resting period followed, during which the testes mass and seminiferous tubules diameter decreased. Spermatogonia and some primary spermatocytes were observed against the wall of seminiferous tubules. The recrudescence period (August, September and October) was characterized by a progressive increase of the testis weight and seminiferous tubules diameter. Spermatocytogenesis began in August, and spermiogenesis occurred in September and October, before the diapause period. However, the presence of spermatozoids was not signaled in the epididymis lumen. Field observations and microscopic evidence of testis showed that both sexes reproduced synchronously during spring and early summer
Cysteine proteases of hookworm Necator Americanus as virulence factors and implications for future drug design: A bioinformatics-based study

Human hookworm Necator Americanus causes iron deficiency anemia, as the parasite ingests blood from the gastrointestinal tract of its human host. The virulence factors of this blood feeding nematode have not been researched extensively. This bioinformatics based study focuses on eight of the cathepsin B like cysteine proteases (CPs) of the worm, which could have immense pathogenic potential. The role of the individual CPs remain vaguely determined except for CP3 which has been shown to act as globinase in the hemoglobin degradation pathway. In this study, the cysteine proteases were subjected to predictive molecular characterizations viz: probability of extracellular secretion to the interface between pathogen and host, ability for hemoglobin degradation, and/or interaction with host plasma proteins. CP1- CP6, which harbored the active site cysteine were also observed to have N terminal signal peptide for extracellular localization, and were relevantly predicted to be secretory. Amongst these, CP2 and CP3 showed the presence of hemoglobinase motif derived in this study that could be a prerequisite for globin or hemoglobin degradation. Active site correlation of the secretory CPs with human pKal that cleaves high molecular weight kininogen (HMWK) to prevent platelet activation is suggestive of the involvement of hookworm CPs in preventing the formation of blood clots via this pathway. NA CP1, CP2, CP3, CP5 and CP6 were predicted to bind heparin, which is the glycosaminoglycan molecule that has been demonstrated to aid the functionality of other cysteine proteases like human cathepsin B and cruzain. Heparin docked onto the NA CPs at the C terminal domain, away from the active site, similar to what has been shown for heparin binding to cathepsin B, and cruzain that cleaves HMWK. These observations therefore lead to the hypothesis that the functions of the hookworm CPs, which would probably include blood clot prevention, could be assisted by heparin. This study underscores the potential of synthetic heparin analogs as molecular treatment for hookworm infection, which could have implications for future drug design.
Conditions for endemicity: qualitative population dynamics in a long-running outbreak of Ebola virus disease

Abstract Ebola virus disease (EVD) struck West Africa in 2013–2016 in an epidemic of unprecedented scope, with over 28000 cases and 11000 fatalities in the affected region. The protracted duration of the outbreak – more than two-and-one-half years of active transmission – raises questions about the persistence of EVD. In this brief paper, we qualitatively examine conditions supporting long-running EVD epidemics via a susceptible – exposed – infectious – recovered – deceased-infectious differential equations model that incorporates births and non disease-related deaths. We define an ‘effective epidemiological population’ to include contagious individuals recently deceased from the disease. Under a constant effective epidemiological population condition, we consider the basic reproductive number and use Lyapunov function arguments to establish conditions in the parameter space supporting an exchange of stability from the disease-free equilibrium to an endemic equilibrium.
Investigation of genomic instability in cholesteatoma of patients

Cholesteatoma is a tumorous keratinocytic hyperproliferation with bony destruction and tissue granulation by chronic inflammation. The cholesteatoma that is named as “skin in the wrong place” is the abnormal existence of keratinized squamous epithelium in the middle ear [1]. Cholesteatoma is invasive, hyperproliferative, recidivistic, expansive and destructive [2]. Cholesteatoma is formed by the effect of inflammatory cytokines, growth factors and/or bacterial toxins. The pathogenetic molecular mechanism of the cholesteatoma is still unclear, although it has been studied for a century. Cholesteatoma is thought as a cell growth disorder which involves an altered control of cellular proliferation. However, the reason of this lack of control is still unknown [3]. Cholesteatoma may also be the results of neoplastic transformation event. It is reported that genetic alterations in cholesteatoma are at the gen level not the chromosomal level [2]. A recent study proposed that the gene expression profile of cholesteatoma is similar to a metastatic tumor. In this study, they demonstrated that the cholesteatoma tissue expresses tomour-relevant genes normally expressed in chronically inflamed tissue [4]. In another recent study, several differentially expressed proteins related with cancer were shown in cholesteatoma comparing to corresponding skin lesions. It was reported that these proteins might cause cancer-like alterations and reorganization of the tissue that may explain the characteristic phenotype of the disease [5]. There are not any medical therapies for cholesteatoma and the only applied treatment now is surgical resection. Even after surgery, recurrences are common in cholesteatoma [6]. The aggressive behavior of a cholesteatoma destroying the surrounding bone may cause life threatening complications such as hearing loss, facial palsy, meningitis or intracranial abscesses and this behavior usually is seen in malignant tumor [7]. Thus, there is a need for developing medical treatment alternatives based on the molecular pathology.
Corporate E-banking: A Study based on DeLone and McLean’s IS Success Model

Although there are ample of studies dealing with retail internet banking, very few studies have investigated corporate internet banking. The objective of the current research is to study the determinants of the intention to continue use online banking of the existing corporate customers. This study collected data from 154 customers of an international bank to test a model developed from the DeLone and McLean’s IS success model. The results show that system quality contributes most to the perception of overall quality of the corporate e-banking service as well as the impact on the future use intention. Other factors that are found to be important are human service quality, information quality, and accuracy and security of the system
Increased CD73 and reduced IFNG signature expression in relation to response rates to anti-PD-1(L1) therapies in EGFR-mutant NSCLC.

11505Background: Anti-PD-1(L1) therapies appear to be less efficacious in NSCLC patients whose tumors have EGFR activating mutations, but the underlying mechanism is poorly understood. We investigated the relationship between Methods: Flow cytometry and/or quantitative PCR were used to evaluate genes and proteins in five NSCLC EGFR mt cell lines and 6 wt lines. Anti-EGFR TKIs gefitinib and osimertinib were used at concentrations ranging from 0.001-100uM; EGF was used at 50 ng/mL. CP1108/NCT01693562 was a nonrandomized phase 1/2 trial evaluating durvalumab (10 mg/kg Q2W) in advanced NSCLC. As of 24OCT16, 304 previously treated patients in CP1108 were enrolled. RNA sequencing was conducted on available tumor specimens from 97 patients in CP1108. CP1108 and TCGA were separated by EGFR status for genomic comparisons. Results: Median CD73 expression was increased 10-fold in EGFR mt NSCLC cell lines (n = 5) compared to wt cell lines (n = 6). EGF induced CD73 protein levels 5-40-fold in 3/6 EGFR wt lines. There .
ULTRASTRUCTURAL STUDY ON THE TEGUMENTAL ALTERATIONS OF MALE AND FEMALE SHISTOSOMA MANSONI Following IN VITRO EXPOSURE TO CURCUMIN

Schistosomes have plagued humans for at least four millennia. Success of infection is mainly attributed to its unique double membrane outer-surface or tegument, which regulates nutritional and immunological interactions between the parasite and the host. This study has been undertaken to evaluate the schistosomicidal activity of curcumin on the ultrastructure of tegument of adult blood fluke, S. mansoni. Whole flukes were exposed in vitro to curcumin for 3 and 6 hours at concentrations of 10, 50 and 100 µM/ml and tegumental changes were examined by scanning electron microscopy. Results revealed that female and male worms treated with curcumin lost their normal surface architecture, since its surface showed destruction in the oral and ventral suckers as well as partial loss of tubercles\' spines, erosion in inter-tubercle tegmental regions and appearance of numerous small blebs around tubercles. The perceived deformations were time and concentration dependent. These findings confirmed that curcumin is a promising therapeutic agent with antischistosomal activity and it may represent a promising alternative treatment for the control of S. mansoni.
Effect of fungal fermentation on the nutrient digestibility of guar meal in Penaeus vannamei

A 30-days indoor feeding trail was carried out to determine the nutrient digestibility of untreated and fermented guar meal (GRM) in Penaeus vannamei . The fermented guar meal was prepared by solid state fermentation using the fungus Aspergillus niger. A reference diet was formulated based on the dietary requirements of shrimp and test diets were prepared using the ingredient substitution method of reference diet and test ingredient at 7:3 ratio. Results revealed that the fungal fermentation significantly (p<0.05) increased dry matter digestibility of GRM from 48.86 to 54.27% and crude protein digestibility from 64.41 to 68.54%. Significant (p<0.05) improvement was observed in the digestibility of all the amino acids with fermented GRM compared to the untreated GRM. The range of essential amino acids digestibility was increased from 59.4-69.6% to 61.5-73.6% post-fermentation. Lysine in essential amino acids and serine in nonessential amino acids showed the highest digestibility in both untreated and fermented GRM whereas a better improvement was observed with arginine (9.79%) and glutamic acid (8.59%) due to fermentation. The present results of increased digestibility parameters in fermented GRM were attributed to the amelioration of anti-nutritional factors and reduction of fibre fractions.
CD4+ Tcells clear virus butaugmentdisease inmiceinfected withrespiratory syncytial virus. Comparison withtheeffects ofCD8+ T cells

SUMMARY Respiratory syncytial (RS)virus-specific Tcell lines werederived fromthespleens ofBALB/cmice primed byintranasal infection withRS virus. Thelines wereexpanded byrepeated antigenic stimulation invitro, andseparated into CD4+andCD8+Tcell-enriched fractions byimmunomagneticadhesion. Theeffects ofpassive transfer ofthese fractions intoRSvirus infected micewere observed. Themostsevere immunopathological changes wereseeninmicereceiving CD4+cells. Transfer ofCD4+,CD8+orbothcell fractions caused RSvirus-infected micetobecomeill andlose weight. Bothcell lines caused anincrease intheseverity oflungpathology (asmonitored by bronchoalveolar lavage) withtheappearance oflunghaemorrhage andpolymorphonuclear cell efflux. Inaddition, recipients ofCD4+cells developed striking pulmonary eosinophilia. InCD4+cell recipients, 5x105cells weresufficient todecrease lungvirus titre, whereas 2x106CD8+cells were needed toproduce asimilar effect. Theunseparated Tcell line andtheCD4+cell fraction secreted significant amounts ofIL-3, IL-4andIL-S(P<0-001). Highlevels ofIL-2wereproduced onlybythe unseparated Tcell line. TheCD8+cell fraction secreted IL-3only. Theresults showthat, cell-for-cell, CD4+cells aremoreanti-viral andmoreimmunopathogenic thanCD8+cells inRSvirus infected mice. Sucheffects mayhavecontributed totheaugmented disease seeninsomeinfants vaccinated against RSvirus.
An Efficient Workflow for Collecting, Entering, and Proofing Field Data: Harnessing Voice Recording and Dictation Software

Anyone who has spent meaningful time in the field appreciates the amount of time and resources spent collecting, transcribing, and proofing data. Here, I describe a data collection, entry, and proofing workflow that only requires one person, is highly efficient, and uses freely available software. I was inspired to design this pipeline while collecting quantitative trait data regularly on thousands of fieldgrown Impatiens capensis Meerb. plants with limited time and personnel.