Miracle-dz/UniFAR-FTUs huggingface dataset:
Revitalising PBL groups: evaluating PBL with study teams.

CONTEXT In problem-based learning (PBL), students are actively engaged with psychological learning principles as activation of prior knowledge, elaboration and organization of knowledge. In their tutorial groups, however, students do not always apply these principles when working with a procedure like the "Seven-Jump" method. To stimulate students to use these principles more often, they were offered another format within a PBL context: PBL with study teams. During the period of self-study, students work on a regular basis in so-called study teams, small groups of 3-4 persons. In these groups they explain to each other their learning outcomes, clarify for each other their problems while studying texts and organize their knowledge to present this to the members of other study teams in their tutorial group. Previous research showed that students spent more time on self-study in a PBL with study team condition than in a traditional PBL context. In this study the achievement as well as appreciation of students participating in a PBL with study teams' environment, is compared with students working in a traditional PBL environment. OBJECTIVES To determine whether PBL with study teams differs from the traditional PBL environment in students' appreciation and study time. METHODS We conducted an experiment in two blocks over two years. Questionnaires were administered to collect data on appreciation and time for self-study. RESULTS Students' appreciation of the two formats did not differ much. The large standard deviations indicate considerable differences in appreciation between individual students. Appreciation was slightly higher in the second experiment when instructions about how to collaborate were less strict. Students devoted twice as many hours studying in the study group format compared with the traditional PBL format. CONCLUSIONS The students indicated that they enjoyed the format but that the increased workload disturbed their customary study rhythm. Assessment scores and tutors'impressions suggest that study teams foster deeper learning for understanding. Whether this can be attributed to more intensive individual and collaborative elaboration activities should be the subject of further studies.
Mechanical Ventilation and Clinical Outcomes in Patients with Acute Myocardial Infarction: A Retrospective Observational Study

Purpose Patients with acute myocardial infarction (AMI) and respiratory impairment may be treated with either invasive or non-invasive mechanical ventilation (MV). However, there has been little testing of non-invasive MV in the setting of AMI. Our objective was to evaluate the incidence and associated clinical outcomes of patients with AMI who were treated with non-invasive or invasive MV. Methods This was a retrospective observational study in which consecutive patients with AMI (n = 1610) were enrolled. The association between exclusively non-invasive MV, invasive MV and outcomes was assessed by multivariable models. Results Mechanical ventilation was used in 293 patients (54% invasive and 46% exclusively non-invasive). In-hospital mortality rates for patients without MV, with exclusively non-invasive MV, and with invasive MV were 4.0%, 8.8%, and 39.5%, respectively (P<0.001). The median lengths of hospital stay were 6 (5.8–6.2), 13 (11.2–4.7), and 28 (18.0–37.9) days, respectively (P<0.001). Exclusively non-invasive MV was not associated with in-hospital death (adjusted HR = 0.90, 95% CI 0.40–1.99, P = 0.79). Invasive MV was strongly associated with a higher risk of in-hospital death (adjusted HR = 3.07, 95% CI 1.79–5.26, P<0.001). Conclusions In AMI setting, 18% of the patients required MV. Almost half of these patients were treated with exclusively non-invasive strategies with a favorable prognosis, while patients who needed to be treated invasively had a three-fold increase in the risk of death. Future prospective randomized trials are needed to compare the effectiveness of invasive and non-invasive MV for the initial approach of respiratory failure in AMI patients.
RENAL SYMPATHETIC BAROREFLEX EFFECTS OF ANGIOTENSIN II INFUSIONS INTO THE ROSTRAL VENTROLATERAL MEDULLA OF THE RABBIT

1. The effects of local infusion of angiotensin II (AII) into the rostral ventrolateral medulla (RVLM) pressor area on the renal sympathetic baroreflex were compared with the excitatory amino acid glutamate in urethane anaesthetized rabbits with chronically implanted renal nerve electrodes. Baroreflex blood pressure‐renal nerve activity curves were obtained by intravenous infusion of phenylephrine and nitroprusside before and after treatments.
Effects of lubricant and autologous bone marrow stromal cell augmentation on immobilized flexor tendon repairs

The purpose of the study was to test a novel treatment that carbodiimide‐derivatized‐hyaluronic acid‐lubricin (cd‐HA‐lubricin) combined cell‐based therapy in an immobilized flexor tendon repair in a canine model. Seventy‐eight flexor tendons from 39 dogs were transected. One tendon was treated with cd‐HA‐lubricin plus an interpositional graft of 8 × 105 BMSCs and GDF‐5. The other tendon was repaired without treatment. After 21 day of immobilization, 19 dogs were sacrificed; the remaining 20 dogs underwent a 21‐day rehabilitation protocol before euthanasia. The work of flexion, tendon gliding resistance, and adhesion score in treated tendons were significantly less than the untreated tendons (p < 0.05). The failure strength of the untreated tendons was higher than the treated tendons at 21 and 42 days (p < 0.05). However, there is no significant difference in stiffness between two groups at day 42. Histologic analysis of treated tendons showed a smooth surface and viable transplanted cells 42 days after the repair, whereas untreated tendons showed severe adhesion formation around the repair site. The combination of lubricant and cell treatment resulted in significantly improved digit function, reduced adhesion formation. This novel treatment can address the unmet needs of patients who are unable to commence an early mobilization protocol after flexor tendon repair. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:154–160, 2016.
Myosin II Regulates Activity Dependent Compensatory Endocytosis at Central Synapses

Recent evidence suggests that endocytosis, not exocytosis, can be rate limiting for neurotransmitter release at excitatory CNS synapses during sustained activity and therefore may be a principal determinant of synaptic fatigue. At low stimulation frequencies, the probability of synaptic release is linked to the probability of synaptic retrieval such that evoked release results in proportional retrieval even for release of single synaptic vesicles. The exact mechanism by which the retrieval rates are coupled to release rates, known as compensatory endocytosis, remains unknown. Here we show that inactivation of presynaptic myosin II (MII) decreases the probability of synaptic retrieval. To be able to differentiate between the presynaptic and postsynaptic functions of MII, we developed a live cell substrate patterning technique to create defined neural circuits composed of small numbers of embryonic mouse hippocampal neurons and physically isolated from the surrounding culture. Acute application of blebbistatin to inactivate MII in circuits strongly inhibited evoked release but not spontaneous release. In circuits incorporating both control and MIIB knock-out cells, loss of presynaptic MIIB function correlated with a large decrease in the amplitude of evoked release. Using activity-dependent markers FM1–43 and horseradish peroxidase, we found that MII inactivation greatly slowed vesicular replenishment of the recycling pool but did not impede synaptic release. These results indicate that MII-driven tension or actin dynamics regulate the major pathway for synaptic vesicle retrieval. Changes in retrieval rates determine the size of the recycling pool. The resulting effect on release rates, in turn, brings about changes in synaptic strength.
Superiority of pentobarbital versus chloral hydrate for sedation in infants during imaging.

PURPOSE To compare the effectiveness and safety of oral pentobarbital and oral chloral hydrate for sedation in infants younger than 1 year during magnetic resonance (MR) imaging and computed tomography (CT). MATERIALS AND METHODS A computerized database was used to collect information about all cases in which sedation was used. Outcomes of all infants who received oral pentobarbital or oral chloral hydrate for sedation between 1997 and 2002 were reviewed. Two study groups were compared for sedation and discharge times by using Student t test and for adverse events by using Fisher exact test and multiple logistic regression analysis. RESULTS Infants (n = 1,316) received an oral medication for sedation. Mean doses were 50 mg/kg chloral hydrate and 4 mg/kg pentobarbital. Student t test demonstrated no difference in mean time to sedation and in time to discharge between groups. Overall adverse event rate during sedation was lower with pentobarbital (0.5%) than with chloral hydrate (2.7%) (P <.001). There were fewer episodes of oxygen desaturation with pentobarbital (0.2%) than with chloral hydrate (1.6%) (P <.01). Both medications were equally effective in providing successful sedation. CONCLUSION Although oral pentobarbital and oral chloral hydrate are equally effective, the incidence of adverse events with pentobarbital was significantly reduced.
CCAAT/enhancer binding protein alpha regulates p21 protein and hepatocyte proliferation in newborn mice

CCAAT/enhancer binding protein alpha (C/EBP alpha) is expressed at high levels in quiescent hepatocytes and in differentiated adipocytes. In cultured cells, C/EBP alpha inhibits cell proliferation in part via stabilization of the p21 protein. The role of C/EBP alpha in regulating hepatocyte proliferation in vivo is presented herein. In C/EBP alpha knockout newborn mice, p21 protein levels are reduced in the liver, and the fraction of hepatocytes synthesizing DNA is increased. Greater than 30% of the hepatocytes in C/EBP alpha knockout animals continue to proliferate at day 17 of postnatal life when cell division in wild-type littermates is low (3%). p21 protein levels are relatively high in wild-type neonates but undetectable in C/EBP alpha knockout mice. The reduction of p21 protein in the highly proliferating livers that lack C/EBP alpha suggests that p21 is responsible for C/EBP alpha-mediated control of liver proliferation in newborn mice. During rat liver regeneration, the amounts of both C/EBP alpha and p21 proteins are decreased before DNA synthesis (6 to 12 h) and then return to presurgery levels at 48 h. Although C/EBP alpha controls p21 protein levels, p21 mRNA is not influenced by C/EBP alpha in liver. Using coimmunoprecipitation and a mammalian two-hybrid assay system, we have shown the interaction of C/EBP alpha and p21 proteins. Study of p21 stability in liver nuclear extracts showed that C/EBP alpha blocks proteolytic degradation of p21. Our data demonstrate that C/EBP alpha regulates hepatocyte proliferation in newborn mice and that in liver, the level of p21 protein is under posttranscriptional control, consistent with the hypothesis that protein-protein interaction with C/EBP alpha determines p21 levels.
131I-Tositumomab Radioimmunotherapy: Initial Tumor Dose–Response Results Using 3-Dimensional Dosimetry Including Radiobiologic Modeling

For optimal treatment planning in radionuclide therapy, robust tumor dose–response correlations must be established. Here, fully 3-dimensional (3D) dosimetry was performed coupling SPECT/CT at multiple time points with Monte Carlo–based voxel-by-voxel dosimetry to examine such correlations. Methods: Twenty patients undergoing 131I-tositumomab for the treatment of refractory B-cell lymphoma volunteered for the study. Sixty tumors were imaged. Activity quantification and dosimetry were performed using previously developed 3D algorithms for SPECT reconstruction and absorbed dose estimation. Tumors were outlined on CT at multiple time points to obtain absorbed dose distributions in the presence of tumor deformation and regression. Equivalent uniform dose (EUD) was calculated to assess the biologic effects of the nonuniform absorbed dose, including the cold antibody effect. Response for correlation analysis was determined on the basis of the percentage reduction in the product of the largest perpendicular tumor diameters on CT at 2 mo. Overall response classification (as complete response, partial response, stable disease, or progressive disease) used for prediction analysis was based on criteria that included findings on PET. Results: Of the evaluated tumor-absorbed dose summary measures (mean absorbed dose, EUD, and other measures from dose-volume histogram analysis), a statistically significant correlation with response was seen only with EUD (r = 0.36 and P = 0.006 at the individual tumor level; r = 0.46 and P = 0.048 at the patient level). The median value of mean absorbed dose for stable disease, partial response, and complete response patients was 196, 346, and 342 cGy, respectively, whereas the median value of EUD for each of these categories was 170, 363, and 406 cGy, respectively. At a threshold of 200 cGy, both mean absorbed dose and EUD had a positive predictive value for responders (partial response + complete response) of 0.875 (14/16) and a negative predictive value of 1.0 (3/3). Conclusion: Improved dose–response correlations were demonstrated when EUD incorporating the cold antibody effect was used instead of the conventionally used mean tumor-absorbed dose. This work demonstrates the importance of 3D calculation and radiobiologic modeling when estimating absorbed dose for correlation with outcome.
Dynamic Security Assertion Markup Language: Simplifying Single Sign-On

Growth in the use of business process outsourcing and collaborative platforms is driving the demand for organizations to selectively share the identity information they maintain about their users with other partners. Widely accepted protocol such as the Security Assertion Markup Language (SAML) are designed to deliver single sign-on (SSO) and other security attributes, but although organizations can gain significant business value by using federated identity management techniques, they continue to face major implementation hurdles (such as wanting to scale from fewer than 10 partners to dozens, hundreds, or even thousands of them). Dynamic SAML takes advantage of security best practices and the exchange of configuration information to minimize the manual steps that administrators must currently perform to configure SAML connections securely. Although it isn't yet possible to completely automate a decision of human trust, dynamic SAML can automate the underlying exchanges to make this decision fast, simple, and secure.
[Efficacy of delayed administration of etanercept after spinal cord injury].

OBJECTIVE To observe the effect of delayed administration of etanercept on the motor function, the expression of apoptosis-related genes and the pathological alterations of spinal cord in vivo in experimental murine model of spinal cord injury (SCI). METHODS Seventy-two male adult SD rats were randomly divided into 6 groups, which were subjected to SCI induced by the application of vascular clips (force of 70 g) to the dura. Experimental groups (E1, E2, and E3 group) were given administration of etanercept immediately, 1 h, and 8 h after SCI. The control groups (C1, C2, and C3 group) were given administration of saline at the same time as experimental groups. Six rats of each group were killed 24 h after SCI in order to collect the samples for testing the expression of Bax and Bcl-2 by Western blot. The rest were killed 14 d after SCI for observing the pathological alteration using light microscopy. The recovery of motor function was graded using the modified murine Basso, Beattle, and Bresnahan (BBB). RESULTS (1) The results of the expressions of Bax and Bcl-2 by Western blot: the gray value of the expression of Bax of E1 group was 165.423 ± 2.946, of E2 group 135.391 ± 3.045, of E3 group 108.543 ± 6.999, and of the control group 69.054 ± 0.774, and the gray value of the expression of Bcl-2 of E1 group was 58.854 ± 3.592, of E2 group 84.315 ± 2.138, of E3 group 125.091 ± 2.699, and of the control group 156.304 ± 2.490. (2) The results of BBB score: etanercept given immediately or 1 h after SCI could improve the recovery of the rats. There were significant differences in BBB score 14 d after SCI between E1 group (13.000 ± 1.095) and C1 group (7.167±0.753), E2 group (9.833 ± 1.472) and C2 group (7.000 ± 0.632) while there were no significant difference between E3 group (7.333 ± 0.516) and C3 group (6.833±0.753). (3) The result of histological alteration: histological alterations, such as necrosis, infiltration of lymphocytes and fibroblast and loss of nerve cells, were found attenuated in E1 and E2 groups, compared with C1 and C2 groups. There was no obvious difference between E3 and C3 groups. CONCLUSION Administration of etanercept may inhibit the apoptosis after SCI, but this kind of effect may be too weak to improve the BBB score and histological alterations obviously when administration of etanercept is delayed 8 h after SCI. The clinical value of etanercept to SCI needs to be further validated.
[Difficulties in emotion regulation and personal distress in young adults with social anxiety].

BACKGROUND The aim of this study was to assess the association between social anxiety and difficulties in emotion regulation in a sample of Italian young adults. METHODS Our convenience sample was composed of 298 Italian young adults (184 women and 114 men) aged 18-34 years. Participants were administered the Interaction Anxiousness Scale (IAS), the Audience Anxiousness Scale (AAS), the Difficulties in Emotion Regulation Scale (DERS), and the Interpersonal Reactivity Index (IRI). A Two Step cluster analysis was used to group subjects according to their level of social anxiety. RESULTS The cluster analysis indicated a two-cluster solution. The first cluster included 163 young adults with higher scores on the AAS and the IAS than those included in cluster 2 (n=135). A generalized linear model with groups as dependent variable indicated that people with higher social anxiety (compared to those with lower social anxiety) have higher scores on the dimension personal distress of the IRI (p<0.01), and on the DERS non acceptance of negative emotions (p<0.001) and lack of emotional clarity (p<0.05). CONCLUSIONS The results are consistent with models of psychopathology, which hypothesize that people who cannot deal effectively with their emotions may develop depressive and anxious disorders.
La place du droit à la protection de la santé au regard du droit constitutionnel français.

Cet article porte sur la notion d’Etat de droit dans le cadre d’un nouvel ordre international. La notion de droit consacre en quelque sorte sa predominance generique au sein de l’Europe et tout particulierement depuis la division physique et ideologique de ce continent. Le droit a la sante souleve quelques questions dans les pays ou ce domaine est completement envahi par les lois et un contexte economique ingrat. Le droit de la sante a ete reconnu par la Constitution francaise en 1946 et desormais on voudrait qu’il soit reconnu comme une obligation par l’Etat et non seulement comme un simple ideal a realiser. La Constitution de 1946, au sujet du droit de la sante a pris force constitutionnelle seulement depuis la decision rendue par le Conseil constitutionnel du 16 juillet 1971. D’abord, il faut voir quelle place aura le droit de la sante dans les parametres constitutionnels en analysant les bases de la Constitution francaise en parallele au droit a la protection de la sante, ainsi que le role et la place qu’a joues l’histoire dans la reconnaissance de ce droit. En effet, la Revolution de 1789 a affirme les droits politiques mais a neglige considerablement les droits sociaux. Ce n’est qu’a l’arrivee de la Constitution du 4 novembre 1848 qu’il y eut un certain elargissement et que les droits sociaux ont eu une poussee fulgurante dans la sphere judiciaire. De plus, la question fondamentale sur le droit universel de la personne humaine sera fort importante dans la denonciation du droit a la sante; plus precisement le caractere recent de la reconnaissance constitutionnelle du droit a la protection de la sante. Il est certes important de prendre en compte ce droit puisqu’il est reconnu et retenu dans le cadre du droit international des droits de l’homme. Alors que le droit a la sante peut etre considere comme un droit autonome et que dans les autres cas il n’est que le corollaire d’un autre droit constitutionnel, il faudra se pencher sur la nature juridique de la protection constitutionnelle de la sante pour en connaitre ses tenants et aboutissants. Finalement, il faudra voir quelle sera la portee de la protection de la sante et la protection du corps humain, soit le principe de la dignite de la personne humaine. Nous constaterons que le Conseil constitutionnel a su mener progressivement le droit a la protection de la sante sur le chemin d’une reconnaissance effective notamment en reconnaissant le caractere essentiel du droit de la sante dans la societe et ce, en passant par la notion de dignite humaine.
Incidence, Persistence, and Determinants of Human Papillomavirus Infection in a Population of Inuit Women in Northern Quebec

Background To study the incidence, persistence, and determinants of human papillomavirus (HPV) infection in a population of Inuit women from Nunavik, Quebec, by overall HPV infection, Alphapapillomavirus (&agr;) species, and oncogenic risk grouping. Methods We recruited a cohort of Inuit women living in communities in Nunavik when they presented for routine care. Baseline sociodemographic and lifestyle characteristics were collected and cervical specimens collected throughout the follow-up period were tested for HPV-DNA using the PGMY Line-blot assay. Results A total of 416 women were eligible for this analysis. Almost 40% of women acquired a new any-type HPV infection, at a rate of 14.44 infections per 1000 women-months. High-risk HPV infections were acquired at a higher rate than low-risk infections and persisted for longer durations. Multivariate logistic regression found age and marital status to be the most important predictors of overall HPV infection acquisition. Over the study period, 36.1% of women cleared their incident infections. The cumulative incidence of any HPV type at 12 months was 10.3% (95% confidence interval, 7.9–13.1). No predictors of clearance were found. Conclusions The high incidence and persistence of HPV infections found demonstrate that this population is at high risk for HPV infection. These data provide a deeper understanding of the HPV infection experience of Inuit women, but they may also help evaluate vaccination strategies currently used for this high-risk population.