Miracle-dz/UniFAR-FTUs huggingface dataset:
Microglial priming through the lung—brain axis: the role of air pollution‐induced circulating factors

Air pollution is implicated in neurodegenerative disease risk and progression and in microglial activation, but the mechanisms are unknown. In this study, microglia remained activated 24 h after ozone (O3) exposure in rats, suggesting a persistent signal from lung to brain. Ex vivo analysis of serum from O3‐treated rats revealed an augmented microglial proinflammatory response and β‐amyloid 42 (Aβ42) neurotoxicity independent of traditional circulating cytokines, where macrophage‐1 antigen‐mediated microglia proinflammatory priming. Aged mice exhibited reduced pulmonary immune profiles and the most pronounced neuroinflammation and microglial activation in response to mixed vehicle emissions. Consistent with this premise, cluster of differentiation 36 (CD36)–/– mice exhibited impaired pulmonary immune responses concurrent with augmented neuroinflammation and microglial activation in response to O3. Further, aging glia were more sensitive to the proinflammatory effects of O3 serum. Together, these findings outline the lung‐brain axis, where air pollutant exposures result in circulating, cytokine‐independent signals present in serum that elevate the brain proinflammatory milieu, which is linked to the pulmonary response and is further augmented with age. —Mumaw, C. L. , Levesque, S. , McGraw, C. , Robertson, S. , Lucas, S. , Stafflinger, J. E. , Campen, M. J., Hall, P., Norenberg, J. P., Anderson, T. , Lund, A. K. , McDonald, J. D. , Ottens, A. K. , Block, M. L. Microglial priming through the lung‐brain axis: the role of air pollution‐induced circulating factors. FASEB J. 30, 1880–1891 (2016). www.fasebj. org
Tumor necrosis factor and interleukin‐6 in spontaneous bacterial peritonitis in cirrhosis: Relationship with the development of renal impairment and mortality

Spontaneous bacterial peritonitis (SBP) is associated with an important production of inflammatory mediators. However, it is unknown whether there is a relationship between the abdominal production of these mediators and the development of renal impairment, one of the most important prognostic parameters in spontaneous bacterial peritonitis. We studied 52 cirrhotic patients at diagnosis and resolution of the infection, by measuring endotoxin, tumor necrosis factor (TNF), and interleukin‐6 (IL‐6) levels in plasma and ascitic fluid. Thirteen patients (25%) developed renal impairment. Patients developing renal impairment showed significantly higher plasma and ascitic fluid cytokine levels at diagnosis of infection than patients who did not (plasma TNF‐α: 96.0 ± 38.7 vs. 39.1 ± 3.6 pg/mL, P = .0209; ascitic fluid TNF‐α: 474.5 ± 118.1 vs. 160.8 ± 42.7 pg/mL, P = .0173; plasma IL‐6: 6,635 ± 2,897 vs. 458 ± 109 pg/mL, P = .0004; ascitic fluid IL‐6: 182,559 ± 47,328 vs. 39,250 ± 10,803 pg/mL, P = .0001). Independent predictors of development of renal impairment at diagnosis were: renal failure (blood urea nitrogen > 30 mg/dL or serum creatinine > 1.5 mg/dL) (P < .001), IL‐6 levels in ascitic fluid (P < .001), and mean arterial pressure (P < .05). Ten of the 13 (77%) patients who developed renal impairment died during hospitalization, but only 2 of the 39 (5%) patients who did not (P= .0001). In addition, renal failure at diagnosis of the infection was the only independent predictor of hospital mortality (P < .001). In conclusion, the inflammatory response to the infection may be an important mechanism of renal impairment and the associated mortality in SBP.
Activating Women in the Labor Market: The Development of South Korea`s Female-Friendly Employment and Labor Market Policies

This paper analyzes the development of female-friendly employment and labor market policies, focusing on the case of South Korea, whose government has pursued an increase in women’s employment in the labor market over the past decade under the pressure of social, economic, and demographic challenges. It examines the three policy cases . Affirmative Action, the creation of good quality part-time employment, and the flexible work arrangement system . , all of which have been actively promoted by the government to assist female workers participating in the labor market. In this paper, I argue that South Korean policy-makers have made various efforts to boost female labor force participation as part of its economic growth strategy, but have achieved very limited outcomes in the improvement of the quality and quantity of women’s employment.
Application of Climate Based Daylight Modelling to the Refurbishment of a School Building in Sicily

This paper aims at promoting the use of Climate Based Daylight Modelling (CBDM) and related state-of-the-art metrics by discussing a range of design options to improve daylight fruition in rooms with different orientation, shape, function, and furniture of an elementary school that is located in the Mediterranean climate of Agira (Italy). The local climatic conditions, with clear skies for most of the year, require the integration of different shading and re-directing systems with the existing envelope and rooms’ layout. Results show that the dynamic modelling is a powerful and ‘creative’ tool in the designer’s hands, which helps to inform about the choice of the most appropriate technological solutions and on their architectural integration. Comparison with mostly used static daylight metrics, such as the average Daylight Factor (aDF) and the Uniformity Ratio (UR), reveals a contrast with what would be suggested if considering these metrics alone, as prescribed by the Italian legislation. These outcomes rebate the need of performing more accurate and dynamic daylight simulations using recorded (i.e., varying) rather than fixed sky conditions to correctly inform the design process.
A Profile of Exemplary Rural Agricultural Entrepreneurship Education Programs

Entrepreneurship in rural areas has been seen as a potential tool to mitigate rural outmigration. Entrepreneurship has long been a part of the comprehensive model for school based agricultural programs in the United States, often emphasized through Supervised Agricultural Experience Programs (SAEP). Using case study methodologies, this study sought to identify programmatic characteristics of exemplary rural agricultural entrepreneurship education programs. Results revealed: (a) entrepreneurship was taught Primarily through SAEP and (b) entrepreneurship was taught a limited amount through coursework. Results also showed that experiential learning related to entrepreneurship was seen primarily through four examples: SAEP, written business plans, scenarios, and Shark Tank type presentations. Recommendations for practice and future research are provided.
Redox regulatory mechanisms in cellular stress responses.

BACKGROUND Reactive oxygen species are produced in a highly localized and specific pattern in biological stress responses. The present review examines the redox regulatory aspects of a number of molecular stress response mechanisms in both prokaryotes and eukaryotes. SCOPE The present review provides examples representing both the cytoplasmic stress response, often studied as the heat shock response, as well as the stress response of the endoplasmic reticulum, known as the unfolded protein response. The examples have been selected to illustrate the variety of ways that redox signals mediate and affect stress responses. CONCLUSIONS Redox regulatory mechanisms are intricately embedded in both the cytoplasmic and endoplasmic reticulum stress responses at multiple levels. Many different stimuli, both internal and external, activate endogenous production of reactive oxygen species as a necessary part of the intracellular communication system that activates stress responses.
Efficacy of topical 5% imiquimod cream for the treatment of nodular basal cell carcinoma: comparison of dosing regimens.

OBJECTIVE To establish a safe and efficacious dosing regimen for the treatment of primary nodular basal cell carcinoma (BCC) using 5% imiquimod cream. DESIGN Two phase 2 studies were conducted: a 6-week, randomized, open-label, dose-response study evaluating 4 dosing regimens and a 12-week, randomized, vehicle-controlled, double-blind, dose-response study evaluating 4 dosing regimens. SETTING Twenty-four public and private dermatology clinics in Australia and New Zealand (6-week study) and the United States (12-week study) participated. PATIENTS The study populations comprised 99 patients enrolled in the 6-week study and 92 patients in the 12-week study. Patients were at least 18 years old and had a biopsy-confirmed diagnosis of nodular BCC. INTERVENTIONS In the 6-week study, imiquimod was applied once daily for 3 or 7 days per week or twice daily for 3 or 7 days per week. In the 12-week study, imiquimod or placebo cream (vehicle) was applied once daily for 3, 5, or 7 days per week, or twice daily for 7 days per week. The entire tumor area was excised 6 weeks after treatment and examined histologically for evidence of remaining BCC. MAIN OUTCOME MEASURE The proportion of patients having no histologic evidence of BCC in the posttreatment excision specimen. RESULTS Dosing once daily for 7 days per week resulted in the highest clearance rate, with 25 (71%) of 35 and 16 (76%) of 21 patients showing clearance of their tumor in the 6- and 12-week studies, respectively. CONCLUSIONS Topical 5% imiquimod cream is well tolerated and most effective in treating nodular BCC when applied once daily for 7 days per week for either 12 or 6 weeks.
Substructure of human von Willebrand factor.

Using electron microscopy, we have visualized the substructure of human von Willebrand factor (vWf) purified by two different approaches. vWf multimers, which appear as flexible strands varying in length up to 2 micron, consist of dimeric units (protomers) polymerized linearly in an end-to-end fashion through disulfide bonds. Examination of small multimers (e.g., one-mers, two-mers, and three-mers) suggests that each protomer consists of two large globular end domains (22 X 6.5 nm) connected to a small central node (6.4 X 3.4 nm) by two flexible rod domains each approximately 34 nm long and approximately 2 nm in diameter. The protomer is 120 nm in length when fully extended. These same structural features are seen both in vWf molecules that were rapidly purified from fresh plasma by a new two-step procedure and in those purified from lyophilized intermediate-purity Factor VIII/vWf concentrates. The 240,000-mol wt subunit observed by gel electrophoresis upon complete reduction of vWf apparently contains both a rod domain and a globular domain and corresponds to one half of the protomer. Two subunits are disulfide-linked, probably near their carboxyl termini, to form the protomer; disulfide bonds in the amino-terminal globular ends link promoters to form vWf multimers. The vWf multimer strands have at least two morphologically distinct types of ends, which may result from proteolytic cleavage in the globular domains after formation of large linear polymers. In addition to releasing fragments that were similar in size and shape to the repeating protomeric unit, plasmic degradation of either preparation of vWf reduced the size of multimers, but had no detectable effect on the substructure of internal protomers.
Teaching by stealth: utilising the hidden curriculum through body painting within anatomy education

This study considers the hidden curriculum within anatomy education, with a specific focus on body painting. Body painting is not only utilised within anatomy education for teaching surface anatomy and clinical examination, but also to provide a platform for the development of other skills, primarily through the hidden curriculum. The hidden curriculum is the unplanned curriculum transmitting tacit messages to students on values, attitudes, principles and organisation. This may lend itself to deliver values in line with General Medical Council UK registration requirements of ‘safety and quality’, ‘communication’, ‘partnership and teamwork’, and ‘maintaining trust’ in the undergraduate medical curriculum. This qualitative study explored faculty perceptions of the use of body painting as a teaching tool. The hidden curriculum appeared spontaneously as a major advantage of utilising painting. Four major themes emerged; trait development, socialisation, tacit learning and script formation. Anatomy education lends itself to an environment in which to study the hidden curriculum. Results from this study demonstrate faculty awareness of, and deliberate use of, the hidden curriculum as a method to ‘teach by stealth’, and therefore by actively employing the term pushing at the boundaries of the hidden curriculum concept.
Entrepreneurial Finance and Economic Growth in the Presence of Trade Unions

This study aims to build a novel setup combining the process of entrepreneurial finance (i.e. the process of search and matching between entrepreneurs and capitalists), with the standard monopoly union framework, within a general endogenous growth model. The idea motivating this research can be described as follows. One the one hand, entrepreneurs need to raise capital for their ventures; on the other hand, capitalists are the ones who provide that capital to the projects they think are the most valuable. Hence, through the process of matching entrepreneurs with capitalists, trade unions might influence this relationship by raising (lowering) wages and, thus, increasing (decreasing) costs and reducing (increasing)
Biology and Developmental Strategies of the Palaearctic Parasitoid Bracon nigricans (Hymenoptera: Braconidae) on the Neotropical Moth Tuta absoluta (Lepidoptera: Gelechiidae)

ABSTRACT During the years after the invasion of Western Palaearctic tomato crops by the South American tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), several indigenous generalist parasitoids have been recorded on this new host. Among these, Bracon nigricans Szépligeti (Hymenoptera: Braconidae) is widely distributed in the Palaearctic region, and has been frequently recovered from South American tomato leafminer. However, most of the available data on this species deal with taxonomic aspects and its recovery in faunistic surveys. The current study documents the biology of B. nigricans on South American tomato leafminer, and provides a scientific basis for its inclusion in South American tomato leafminer management programs in Afro-Eurasia. We studied several B. nigricans life-history traits relevant to juvenile development and reproduction by females. B. nigricans proved to be an idiobiont and a synovigenic ectoparasitoid of mature South American tomato leafminer larvae. Several B. nigricans reproductive traits were influenced by the age of the mothers; on average, the progeny had a slightly male-biased sex ratio (60% males) and a low rate of successful development (≈37%). Adult females lived 42.8 d and produced an average of 29.8 offspring. These biological parameters yielded an intrinsic rate of increase of 0.052. Females showed behavioral plasticity in host use, as initial host paralysis was followed by host-feeding, oviposition, or host rejection, with a consequent high mortality rate among hosts exposed to parasitism. Although further field studies are needed, these data, obtained in laboratory conditions, suggest that B. nigricans should be considered as a potential biological control agent of in newly invaded areas of the Palaearctic region.
Stimulation of intrachromosomal homologous recombination in mammalian cells by an inhibitor of poly(ADP-ribosylation).

We determined the effect of 3-methoxybenzamide (3-MB), a competitive inhibitor of poly(ADP-ribose) polymerase (E.C. 2.4.2.30), on intrachromosomal homologous recombination in mouse Ltk- cells. We used a cell line that contained in its genome two defective Herpes thymidine kinase (tk) genes as closely linked direct repeats. Intrachromosomal homologous recombination events were monitored by selecting for tk-positive segregants that arose during propagation of the cells and recombination rates were determined by fluctuation analysis. We found that growth of cells in the continuous presence of 2mM 3-MB increased intrachromosomal recombination between 3 and 4-fold. Growth of cells in the presence of 2mM m-anisic acid, a non-inhibitory analog of 3-MB, had no effect on intrachromosomal recombination rates. Additionally, we found that 3-MB increased both gene conversions and crossovers to similar extents, adding to the evidence that these two types of intrachromosomal rearrangements share a common pathway. These findings contrast with our previous studies [Waldman, B.C. and Waldman, A.S. (1990) Nucleic Acids Res., 18, 5981-5988] in which we determined that 3-MB inhibits illegitimate recombination and has no effect on extrachromosomal homologous recombination in mouse Ltk- cells. An hypothesis is offered that explains the influence of 3-MB on different recombination pathways in mammalian cells in terms of the role that poly(ADP-ribosylation) plays in DNA break-repair.
Do illiquid stocks jump more frequently?

ABSTRACT We study the influence of stock liquidity on the stock price jump frequency using intraday data of 175 US stocks during 2007–11. Grouping these stocks according to their average liquidity we find less liquid stocks to jump more often than liquid stocks. Depending on the liquidity measure the least liquid stocks exhibit on average between 10% and 34% more jumps than the most liquid stocks. Our results are robust to different definitions of liquidity and jump measures as well prevail under different time frequencies.
Characterisation of a Novel Series of Aprotinin-Derived Anticoagulants

Summary Previous investigations have indicated that interference with the initial level of the blood coagulation may lead to effective antithrombotic therapy. Recently a series of potential coagulation inhibitors derived from bovine pancreatic trypsin inhibitor (BPTI, aprotinin) was described. We have determined their inhibition constants, effects on coagulation assays, effects in an in vitro human thrombosis model and pharmacological profiles in hamsters. The aprotinin-derived analogues (4C2,7L22, 5L15, 6L15, 5L84) showed significantly increased inhibitory activity towards factor Xa, factor Vlla-tissue factor (TF) complex, factor XIa and plasma kallikrein or a combination of them, and a significantly decreased plasmin inhibition as compared to aprotinin. In the coagulation assays, 4C2 and 7L22 mainly inhibited factor Xa, 5L15 and 6L15 inhibited factor VIIa-TF complex and 5L84 inhibited factor Xa, factor VIIa-TF complex and the contact activation. In flow chamber experiments with human blood 7L22, 5L15, 6L15, 5L84 and rTAP significantly inhibited fibrin formation and platelet deposition on extracellular matrix from phorbol ester stimulated human endothelial cells both under high and low shear stress and in the presence of low molecular weight heparin. The pharmacological profiles of the aprotinin analogues and rTAP with a mean residence time of 64 to 140 min were not significantly different. Modification of an aprotinin analogue with PEG (5L15-PEG) resulted in a 10-fold decrease of the inhibition constant for the factor VIIa-TF complex and in a significant prolongation of the secondary half-life, while the initial half-life was unchanged. Thus the investigated aprotinin-derived coagulation inhibitors resulted in a series of combined coagulation inhibitors with a pharmacological behaviour, which justifies in vivo testing of their potential antithrombotic action, as reported in the accompanying paper.
Tumor Necrosis Factor- α Blocker in Treatment of Juvenile Idiopathic Arthritis-Associated Uveitis Refractory to Second-line Agents: Results of a Multinational Survey

Objective. Uveitis occurs in 10%–15% of patients with juvenile idiopathic arthritis (JIA). If topical treatment fails, second-line agents are used to control the disease. However, some patients need the addition of tumor necrosis factor- α (TNF- α ) antagonist (anti-TNF). We organized a cross-sectional cohort to investigate use and efficacy of anti-TNF treatment in patients with JIA-associated uveitis. Methods. The international pediatric rheumatology community was queried about the use and efficacy of anti-TNF in treatment of JIA-associated uveitis using an E-mail survey. Results. Of the 33 responding centers following 884 patients with uveitis, only 15 centers, following 404 patients, were using anti-TNF for this indication. A total of 47 patients with JIA-related uveitis treated with anti-TNF because of an insufficient response to previous therapy were reported. The mean age of the patients was 12.5 years. The mean duration from onset of uveitis to start of anti-TNF treatment was 45.1 months. Three different anti-TNF agents were used: etanercept in 34 cases, infliximab in 25 cases, and adalimumab in 3 cases. In 12 of the 34 patients etanercept was inefficacious and patients were switched to infliximab. The final response was rated according to a composite index as 53%/12%/32%, and according to physician rating as 47%/12%/38% representing good, moderate, and poor, respectively, in the etanercept group; and 70%/30%/0% and 68%/24%/0% in the infliximab group. All 3 patients taking adalimumab were responders. Infliximab was statistically significantly more efficacious for the treatment of JIA-associated uveitis than etanercept (chi-square p = 0.004). Conclusion. Anti-TNF seems to be an effective treatment for refractory JIA-associated uveitis. In this cohort infliximab was more efficacious etanercept.
Enhanced magnetic fluid hyperthermia by micellar magnetic nanoclusters composed of Mn(x)Zn(1-x)Fe(2)O(4) nanoparticles for induced tumor cell apoptosis.

Monodispersed MnxZn1-xFe2O4 magnetic nanoparticles of 8 nm are synthesized and encapsulated in amphiphilic block copolymer for development of the hydrophilic magnetic nanoclusters (MNCs). These MNCs exhibit superparamagnetic characteristics, high specific absorption rate (SAR), large saturation magnetization (Ms), excellent stability, and good biocompatibility. MnFe2O4 and Mn0.6Zn0. 4Fe2O4 are selected as optimum compositions for the MNCs (MnFe2O4/MNC and Mn0.6Zn0.4Fe2O4/MNC) and employed for magnetic fluid hyperthermia (MFH) in vitro. To ensure biosafety of MFH, the parameters of alternating magnetic field (AMF) and exposure time are optimized with low frequency, f, and strength of applied magnetic field, Happlied. Under optimized conditions, MFH of MnFe2O4/MNC and Mn0.6Zn0. 4Fe2O4/MNC result in cancer cell death rate up to 90% within 15 min. The pathway of cancer cell death is identified as apoptosis, which occurs in mild hyperthermia near 43 °C. Both MnFe2O4/MNC and Mn0.6Zn0. 4Fe2O4/MNC show similar efficiencies on drug-sensitive and drug-resistant cancer cells. On the basis of these findings, those MnxZn1-xFe2O4 nanoclusters can serve as a promising candidate for effective targeting, diagnosis, and therapy of cancers. The multimodal cancer treatment is also possible as amphiphilic block copolymer can encapsulate, in a similar fashion, different nanoparticles, hydrophobic drugs, and other functional molecules.