Miracle-dz/UniFAR-FTUs huggingface dataset:
MiR-421 inhibition protects H9c2 cells against hypoxia/reoxygenation-induced oxidative stress and apoptosis by targeting Sirt3

Background: MicroRNAs (miRNAs) are involved in myocardial ischemia-reperfusion injury. miRNA-421 (miR-421) plays a significant role in the initiation of apoptosis and myocardial infarction. However, the molecular regulation of miR-421 in myocardial ischemia-reperfusion injury requires further elucidation. Methods: An in vitro hypoxia/reoxygenation model was established, and the expression levels of miR-421 and Sirtuin-3 (Sirt3) in H9c2 cells were quantified using quantitative real-time polymerase chain reaction. Flow cytometry was employed to measure the effects of miR-421 on myocardial apoptosis induced by hypoxia/reoxygenation. The activity of lactate dehydrogenase and superoxide dismutase and levels of malondialdehyde were measured. The binding sites of miR-421 on Sirt3 were predicted using TargetScan software. A luciferase reporter assay was used to validate the direct targeting of Sirt3 with miR-421. Protein expression levels of Sirt3 and its downstream proteins were evaluated using Western blot analysis. Results: Exposure of H9c2 cells to hypoxia/reoxygenation led to increased apoptosis, levels of malondialdehyde and lactate dehydrogenase, and decreased levels of superoxide dismutase. miR-421 knockdown resulted in decreased apoptosis, levels of lactate dehydrogenase and malondialdehyde, and increased superoxide dismutase levels in H9c2 cells. Hypoxia/reoxygenation significantly decreased the relative expression levels of Sirt3. Down-regulation of Sirt3 resulted from overexpression of miR-421, which directly targeted Sirt3. Knockdown of miR-421 up-regulated Sirt3 expression, inhibited activation of the Jun N-terminal kinase/activator protein 1 pathway and caspase 9/3-dependent cell death. Conclusion: The miR-421-Sirt3-Jun N-terminal kinase/activator protein 1 axis is a novel molecular mechanism that accommodates hypoxia/reoxygenation-induced oxidative stress and apoptosis and provides a new direction for the study and treatment of hypoxia/reoxygenation.
The role of diet in irritable bowel syndrome: implications for dietary advice

Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder that affects approximately 10% of the population. Diet triggers symptoms in the vast majority of individuals with IBS. In view of this, there has been a focus on the role of diet in IBS. The diets currently being headlined for IBS include (i) traditional dietary advice, (ii) the low fermentable oligo‐, di‐, mono‐ saccharides and polyols (FODMAPs) diet and (iii) the gluten‐free diet (GFD). Although traditional dietary advice is considered as the first‐line dietary therapy, its evidence base is variable, with a few randomized controlled trials (RCTs) exploring the efficacy of this approach, other than for fibre. There are now a growing number of RCTs demonstrating the efficacy of the low FODMAP diet in the short‐term, with some emerging data on the long‐term ‘adapted’ low FODMAP diet. There are also several RCTs showing the benefits of a GFD in IBS; however, this concept is hampered with uncertainty as to the mechanism of action. Nevertheless, all of these dietary therapies are viable options for individuals with IBS, with the dietitian and patient engagement at the forefront of achieving success. However, future pragmatic studies are needed to clarify the comparative efficacy and convenience of implementing these various diets into routine life. Moreover, it is imperative to better delineate the concern that restrictive diets – such as the low FODMAP and GFD – may promote nutritional inadequacies, disordered eating behaviours, and lead to detrimental alterations to the gut microbiota.
Contemporary Outcomes for Advanced-Stage Classical Hodgkin Lymphoma in the U.S.: Analysis of Surveillance, Epidemiology, and End Results Database.

BACKGROUND Advanced-stage Hodgkin lymphoma (HL) is a curable malignancy, although outcomes remain poor in certain patients. It remains unclear if recent advances have improved their population-level survival over time. MATERIALS AND METHODS Using the Surveillance, Epidemiology, and End Results database, we identified patients aged ≥18 years with stage III or IV classical HL as the first primary malignancy, diagnosed between 2000 and 2014 and treated with chemotherapy. Patients were stratified by date of diagnosis into three groups (2000-2004, 2005-2009, 2010-2014) to assess the trends in overall survival (OS). RESULTS A total of 9,042 patients with a median age of 41 years were included. The use of frontline radiation therapy decreased in each period (21.3% [2000-2004] vs. 15.5% [2005-2009] vs. 10.7% [2010-2014]; p < .001). Three-year OS was significantly higher for patients diagnosed between 2010 and 2014 (81.8%) and 2005 and 2009 (80.6%) compared with 2000 and 2004 (78.5%; p = .0008 and .02, respectively). Whereas outcomes were poorest in the age >60 cohort, similar improvements were also seen in 3-year OS over the three time periods within this patient population. On multivariate analysis, diagnosis in the earlier period and minority race were associated with higher mortality. Females and married patients had significantly lower mortality risk. CONCLUSION Survival of patients with advanced-stage HL has continued to improve over time, suggesting the impact of evolving treatment approaches. Three-year OS in the contemporary period remains inadequate at 81.8%, highlighting the need for continued research to improve their outcomes. IMPLICATIONS FOR PRACTICE This article evaluates contemporary outcomes for advanced-stage Hodgkin lymphoma (HL) in the U.S. using the Surveillance, Epidemiology, and End Results database. Although overall survival (OS) has improved in each 5-year period since 2000, the 3-year OS from 2010 to 2014 remains inadequate at 81.8% and is limited by patient demographics. New therapies are indicated to improve clinical outcomes in advanced-stage HL.
Burst Phenomenon Analysis in Social Tagging System using Hawkes Process

In the activities of people on WEB such as SNS, the burst phenomenon is observed. Recently, Hawkes Process is used as a method to analyze the burst phenomenon. It is known that when the branching ratio, which is an index representing the internal dynamics of Hawkes Process, exceeds a certain threshold, the event time series transits from steady state to nonstationary state where the burst phenomena is likely to occur. In this paper, we focus on social tagging system among data obtained from SNS, and analyze how branching ratio changes timewise with service growth.
The Role of Neutrophils and Neutrophil Extracellular Traps in Vascular Damage in Systemic Lupus Erythematosus

Systemic lupus erythematosus (SLE) is an autoimmune syndrome of unknown etiology, characterized by multi-organ inflammation and clinical heterogeneity. SLE affects mostly women and is associated with a high risk of cardiovascular disease. As the therapeutic management of SLE improved, a pattern of early atherosclerotic disease became one of the hallmarks of late disease morbidity and mortality. Neutrophils emerged as important players in SLE pathogenesis and they are associated with increased risk of developing atherosclerotic disease and vascular damage. Enhanced neutrophil extracellular trap (NET) formation was linked to vasculopathy in both SLE and non-SLE subjects and may promote enhanced coronary plaque formation and lipoprotein dysregulation. Foundational work provided insight into the complex relationship between NETs and immune and tissue resident cells within the diseased artery. In this review, we highlight the mechanistic link between neutrophils, NETs, and atherosclerosis within the context of both SLE and non-SLE subjects. We aim to identify actionable pathways that will drive future research toward translational therapeutics, with the ultimate goal of preventing early morbidity and mortality in SLE.
Reliability and validity of Turkish version of pregnancy physical activity questionnaire (PPAQ) in patients with gestational diabetes mellitus*

Abstract Gestational diabetes mellitus (GDM) is a common complication during pregnancy. Evaluation of the quantitative physical activity in diabetic pregnant women is critical. The aim of this study was to test the reliability and validity of the Pregnancy Physical Activity Questionnaire (PPAQ) in Turkish patients with GDM. A total of 120 pregnant women between the ages of 18 and 44 years with GDM were included. The reliability of the questionnaire was measured by internal consistency and analysis of 2-week test-retest reliability. Of the patients, 74 completed the test-retest procedure. Concurrent validity was examined by comparing the PPAQ with the Short Form of the International Physical Activity Questionnaire (IPAQ) in 36 patients. Test-retest intraclass correlation coefficient scores varied between 0.72 and 0.95. The Spearman rank correlation analysis showed that the PPAQ total activity values were statistically significantly correlated with the total values of IPAQ-Short Form (r = 0.410 and p = .030). In conclusion, the Turkish version of the PPAQ is a valid and reliable tool for the measurement of the physical activity level of pregnant women with GDM. Impact statement What is already known on this subject? The pregnancy physical activity questionnaire (PPAQ) developed in 2004 by Chasan-Taber et al.; is a simple and short questionnaire measuring the frequency, duration, and intensity of physical activity in pregnant women. To date, this questionnaire has been translated into many languages and has been used in a number of studies. What do the results of this study add? The aim of this study was to test the reliability and validity of the PPAQ in Turkish pregnant women with GDM. On the basis of our study results, we suggest that the Turkish version of the PPAQ is a valid and reliable tool for the measurement of the physical activity level of pregnant women with GDM. What are the implications of these findings for clinical practice and/or further research? Evaluation of the quantitative physical activity in diabetic pregnant women may contribute to gain a better understanding of the role of physical activity during treatment and may be useful to compare the results of different studies carried out in different places more effectively.
Modern English Grammar

A great many people seem to think that the study of grarmmar is a very dry subject indeed, but that it is extremely useful, assisting the pupils in writing and in speaking the language in question. Now I hold the exactly opposite view. I think that the study of grammar is really more or less useless, but that it is extremely fascinating. I don't think that the study of grammar, at least in the way in which grammar has been studied hitherto, has been of very material assistance to any one of the masters of English prose or poetry, but I think that there are a great many things in grammar that are interesting and that can be made interesting to any normal schoolboy or schoolgirl. The chief thing is not to approach grammar from the side of logic or abstract definitions. What is wanted is to show that language is a living thing and what that means. When children begin to learn about cats and dogs they don't start with the definition of what a cat is or what a dog is, but they learn that this animal, which is very interesting to them, is a cat, and that this other animal, which is perhaps even more interesting to them, is a dog, and then perhaps after many years they will advance so far in their study of zoology that they would be asked in an examination the question, "How would you define a cat? " or "How would you define a dog? "though I don't believe that even in the case of zoology you would think of asking that sort of question. Now, then, why should we start with definitions of nouns, adjectives, and verbs, and all these things? I don't see that there is any reason in that.
Cyber Dating Abuse Victimization: Links With Psychosocial Functioning

It is well established that technology can be used to enact intimate partner violence (IPV). However, less is known about how cyber dating abuse (CDA) is associated with psychosocial functioning, especially when accounting for other forms of frequently co-occurring IPV victimization. The current study sought to determine the unique associations of CDA victimization when controlling for multiple forms of in-person IPV victimization. Two hundred seventy-eight men and women between 17 and 25 years of age (M = 20.5, SD = 1.9) who were currently in an intimate relationship for at least 3 months participated in this study. Participants completed questionnaires about their IPV and CDA victimization, as well as a range of indices of psychosocial well-being. Experiencing CDA victimization was related to increased alcohol use for both men and women, and increased fear of partner for women, even after controlling for in-person IPV. For depression, perceived stress, relationship satisfaction, quality of life, social support, and post-traumatic stress, CDA victimization did not predict levels above in-person IPV victimization. Although these results suggest some unique associations between CDA victimization and aspects of psychosocial well-being that require further attention, they also highlight that CDA often occurs within a broader pattern of abuse that includes in-person IPV. These results suggest that the need for prevention and treatment for relationships that involve in-person abuse is still most salient, and that a narrow focus on CDA may limit the utility of prevention and treatment efforts. Further work is needed to integrate research on in-person and CDA victimization, rather than to create a new field of research and practice based solely on CDA.
EORTC SPECTA‐AYA: A unique molecular profiling platform for adolescents and young adults with cancer in Europe

For most adolescent and young adult (AYA) cancers, age‐specific molecular features are poorly understood. EORTC‐SPECTA, an academic translational research infrastructure for biomaterial collection, will explicitly recruit AYA patients and will therefore collect empirical data to bridge the molecular gap between pediatric and adult oncology. The initial pilot study, activated in February 2019 across Europe, will recruit 100 AYA patients (aged 12–29 years) with newly diagnosed or relapsed high‐grade gliomas and high‐grade bone and soft tissue sarcomas. The primary objective of the pilot is to determine feasibility and recruitment rates. Formalin‐fixed tumor tissue and whole blood from study participants will be prospectively collected with clinical data and stored centrally at the Integrated BioBank of Luxembourg. Whole exome sequencing of matched tumor and blood, and tumor RNA sequencing and DNA methylation profiling will be performed at the German Cancer Research Center, Heidelberg, Germany. Virtual central pathology review of scanned diagnostic slides will be undertaken by an international expert panel, and diagnostic material returned to the participating centers. A multidisciplinary molecular tumor board will release a clinically validated report to referring clinicians within 4–6 weeks after biopsy. SPECTA‐AYA constitutes a major opportunity to gain knowledge about the tumor biology of this unique age group. It incorporates notable innovative aspects: AYA specificity, pan‐European academic collaboration, centralized biobanking, comprehensive molecular profiling and virtual central pathology review, among others. SPECTA‐AYA will help untangle the tumor particularities of AYAs with cancer and aims to improve their access to novel drugs and personalized medicine.
Perencanaan Perluasan Coverage Area Jaringan Lte Menggunakan Physical Cell Identity (pci) Di Balubur Town Square Bandung

Komunikasi selluler pada saat ini merupakan salah satu yang paling penting di era modern ini. Pada saat ini komunikasi selluler di Indonesia sudah sampai pada generasi ke 4 (4G) atau yang sering kita dengar dengan nama LTE (Long Term Evolution). Dengan menggunakan teknologi LTE dapat memudahkan semua orang dalam mengakses data dengan kecepatan tinggi. Khususnya Balubur Town Square Bandung yang merupakan salah satu pusat perniagaan terbesar di kota Bandung yang banyak didatangi pengunjung dalam dan luar kota Bandung. Dengan jumlah pengunjung yang meningkat, berakibat pada kualitas sinyal dan coverage di dalam mall yang mengalami penurunan level daya terima, sehingga performansi sinyal menjadi turun. Sehingga perlu dilakukannya perencanaan jaringan LTE. Pada penelitian tugas akhir ini telah dilakukan perbandingan antara DAS (Distributed antenna System) dengan teknologi Indoor Pico (Lampsite) untuk perluasan coverage dan capacity dengan menggunakan physical cell identity (pci) . Physical cell identity (pci) merupakan salah satu parameter dengan nilai antara 0-503 tiap transmitter untuk mengirimkan informasi ke tiap pengguna cell tertentu agar tidak terjadi interferensi. Alokasi Physicall Cell Identiy pada penomoran PCI ini menggunakan 142 s/d 167 secondary synchronization signall. Dari hasil perhitungan yang telah dilakukan teknologi lampsite mempunyai coverage area yang lebih besar yang menyebabkan estimasi cell pada lampsite lebih sedikit yaitu Lantai dasar 1 14 Lampsite,Lantai dasar 2 14 Lampsite, lower ground 14 Lampsite, upper ground 14 Lampsite ,lantai 1 7 Lampsite dan hasil simulasi untuk tiap lantai di dapatkan nilai RSL -50,83 dBm, -56,74 dBm,-52,09 dBm, -51,67 dB dan -56,04 dBm. Untuk nilai SIR diperoleh hasil simulasi dari 5 lantai adalah 13.12 dB ,21.03 dB, 18.85 dB, 16.84 dB dan 11.79 dB. Dari hasil simulasi yang diperoleh, perencanaan jaringan indoor LTE telah memenuhi KPI ( Key Performance Indicator ) LTE indoor planning yang digunakan oleh telkomsel.
The Common Partner of Several Methyltransferases TRMT112 Regulates the Expression of N6AMT1 Isoforms in Mammalian Cells

Methylation is a widespread modification occurring in DNA, RNA and proteins. The N6AMT1 (HEMK2) protein has DNA N6-methyladenine as well as the protein glutamine and histone lysine methyltransferase activities. The human genome encodes two different isoforms of N6AMT1, the major isoform and the alternatively spliced isoform, where the substrate binding motif is missing. Several RNA methyltransferases involved in ribosome biogenesis, tRNA methylation and translation interact with the common partner, the TRMT112 protein. In this study, we show that TRMT112 regulates the expression of N6AMT1 isoforms in mammalian cells. Both isoforms are equally expressed on mRNA level, but only isoform 1 is detected on the protein level in human cells. We show that the alternatively spliced isoform is not able to interact with TRMT112 and when translated, is rapidly degraded from the cells. This suggests that TRMT112 is involved in cellular quality control ensuring that N6AMT1 isoform with missing substrate binding domain is eliminated from the cells. The down-regulation of TRMT112 does not affect the N6AMT1 protein levels in cells, suggesting that the two proteins of TRMT112 network, WBSCR22 and N6AMT1, are differently regulated by their common cofactor.
Endocrine responses during CPAP withdrawal in obstructive sleep apnoea: data from two randomised controlled trials

The aim of this investigation was to elucidate the effect of CPAP withdrawal on neurometabolic and cardiometabolic markers in patients with obstructive sleep apnoea. We evaluated 70 patients (mean age 61±10 years, 82% men) treated with CPAP in two 2-week, parallel, randomised controlled trials. CPAP withdrawal resulted in elevated 3,4-dihydroxyphenylglycol, norepinephrine and cortisol after 2 weeks of CPAP withdrawal; however, no statistically significant changes of the renin–angiotensin–aldosterone system (RAAS) determinants were documented. In summary, CPAP withdrawal may be more prominently linked to short-term increases in sympathetic activation than hypothalamic–pituitary–adrenal axis or RAAS activation. ClinicalTrials.gov Identifier: NCT02493673 and NCT02050425.
A proposal for classification of oropharyngeal squamous cell carcinoma: morphology and status of HPV by immunohistochemistry and molecular biology.

The current three-tier grading system (well, moderate and poorly differentiated) used to morphologically classify head and neck squamous cell carcinoma (HNSCC) is inadequate for categorisation of oropharyngeal squamous cell carcinoma (OPSCC) owing to the lack of prognostic value. The aim of this study was to assess the validity of a classification system for OPSCC based on morphology and HPV infection status. Haematoxylin and eosin slides of 121 patients (100 M, 21 F, age range 40-89 years) with OPSCC were reviewed and categorised as histological types I, II, and III. The presence of HPV was assessed by immunohistochemistry with p16 and RNAscope In Situ Hybridization (ISH). The follow-up period was 36 months. 96 patients were p16+ and clinical stage I. Patient survival with types I, II, and III was 93%, 50%, and 96%, respectively. 25 patients were p16-: 10 clinical stage I and 15 stage III. Amongst this group no type I morphology was identified. At follow-up 65% of type II and 75% of type III patients were alive. All p16+ cases were also positive for E6/E7 mRNA high risk-HPV by ISH, while 23 p16- cases were negative and two were positive. Cox regression identified three predictors of mortality: older age (HR = 1.14, 95% CI = 1.06-1.23, p = 0.001); female gender (HR = 0.22.95% CI 0.05-0.88, p = 0.033) and type II morphology (HR = 13.1, 95% CI = 1.09-157.0, p = 0.043). OPSCC morphological classification in three sub-types, along with HPV infection status, seems to reflect the outcome of patients with OPSCC. This article is protected by copyright. All rights reserved.
Sequence‐based mapping identifies a candidate transcription repressor underlying awn suppression at the B1 locus in wheat

Summary Awns are stiff, hair‐like structures which grow from the lemmas of wheat (Triticum aestivum) and other grasses that contribute to photosynthesis and play a role in seed dispersal. Variation in awn length in domesticated wheat is controlled primarily by three major genes, most commonly the dominant awn suppressor Tipped1 (B1). This study identifies a transcription repressor responsible for awn inhibition at the B1 locus. Association mapping was combined with analysis in biparental populations to delimit B1 to a distal region of 5AL colocalized with QTL for number of spikelets per spike, kernel weight, kernel length, and test weight. Fine‐mapping located B1 to a region containing only two predicted genes, including C2H2 zinc finger transcriptional repressor TraesCS5A02G542800 upregulated in developing spikes of awnless individuals. Deletions encompassing this candidate gene were present in awned mutants of an awnless wheat. Sequence polymorphisms in the B1 coding region were not observed in diverse wheat germplasm whereas a nearby polymorphism was highly predictive of awn suppression. Transcriptional repression by B1 is the major determinant of awn suppression in global wheat germplasm. It is associated with increased number of spikelets per spike and decreased kernel size.
Sexually transmitted hepatitis C virus infections: current trends, and recent advances in understanding the spread in men who have sex with men

Hepatitis C virus (HCV) is a major public health threat. Although the recent availability of highly effective directly acting antivirals created optimism towards HCV elimination, there is ongoing transmission of HCV in men who have sex with men (MSM). We here report current epidemiological trends and synthesise evidence on behavioural, network, cellular and molecular host factors associated with sexual transmission of HCV, in particular the role of HIV‐1 co‐infection. We discuss prevention opportunities focusing on the potential of HCV treatment.