Miracle-dz/UniFAR-FTUs huggingface dataset:
Intratumoral accumulation of podoplanin-expressing lymph node stromal cells promote tumor growth through elimination of CD4+ tumor-infiltrating lymphocytes

ABSTRACT The beneficial effects of checkpoint blockade in tumor immunotherapy are limited to patients with increased tumor-infiltrating lymphocytes (TILs). Delineation of the regulatory networks that orchestrate the presence of TILs holds great promise for the design of effective immunotherapies. Podoplanin/gp38 (PDPN)-expressing lymph node stromal cells (LNSCs) are present in tumor stroma; however, their effect in the regulation of TILs remains elusive. Herein we demonstrate that intratumor injection of ex-vivo-isolated PDPN+ LNSCs into melanoma-bearing mice induces elimination of TILs and promotes tumor growth. In support, PDPN+ LNSCs exert their function through direct inhibition of CD4+ T cell proliferation in a cell-to-cell contact independent fashion. Mechanistically, we demonstrate that PDPN+ LNSCs mediate T cell growth arrest and induction of apoptosis to activated CD69+CD4+ T cells. Importantly, LTbR-Ig-mediated blockade of PDPN+ LNSCs expansion and function significantly attenuates melanoma tumor growth and enhances the infiltration and proliferation of CD4+ TILs. Overall, our findings decipher a novel role of PDPN-expressing LNSCs in the elimination of CD4+ TILs and propose a new target for tumor immunotherapy.
Letter regarding article by Stettler et al, "Weight gain in the first week of life and overweight in adulthood: a cohort study of European American subjects fed infant formula".

To the Editor: Recently, Stettler et al1 published exciting data from a large cohort of formula-fed infants on the association between weight gain in the first neonatal week and risk of overweight in adulthood. Suggesting that this early, short period has such a long-term impact on later health is fascinating and provoking. We have data strongly …
Dietary ratio of animal:plant protein is associated with 24-h urinary iodine excretion in healthy school children

Adequate dietary iodine intake in children is essential for optimal physical and neurological development. Whether lower dietary animal food and salt intake may adversely affect iodine status is under discussion. We examined the association between dietary animal:plant protein ratio with 24-h urinary iodine excretion (24-h UI, μg/d), and whether this is modified by salt intake. A 24-h UI was measured in 1959 24-h urine samples from 516 6- to 12-year-old participants of the Dortmund Nutritional and Anthropometric Longitudinally Designed Study. Parallel 3 d weighed food records were used to estimate dietary intakes. Protein sources were classified as dairy, animal and plant. A repeated-measures regression model (PROC MIXED) was used to analyse the effect of animal:plant protein ratios on 24-h UI. Animal:plant protein ratios ranged from 0·5 (95 % CI 0·4, 0·6) to 1·6 (95 % CI 1·4, 1·9) (lowest and highest quartile). After adjustment for total energy intake, main dietary iodine sources (dairy and salt intake), and further covariates, the inter-individual variation in animal:plant protein ratio was significantly associated with variation in 24-h UI. One unit higher animal:plant protein ratio predicted 6 μg/d higher 24-h UI (P= 0·002) in boys and 5 μg/d (P= 0·03) in girls. This relationship was partially mediated by a higher salt intake at higher animal:plant protein ratios. These results suggest that lower consumption of animal protein is associated with a small decline in iodine excretion, partially mediated by decreased salt intake. Because limited salt and increased intake of plant-based foods are part of a preferable healthy food pattern, effective nutrition political strategies will be required in the future to ensure appropriate iodine nutrition in adherent populations.
Ribosomal initiation from an ACG codon in the Sendai virus P/C mRNA.

The Sendai virus P/C mRNA expresses the P and C proteins from alternate reading frames. The C reading frame of this mRNA, however, is responsible for three proteins, C‘, C and Y, none of which appear to be precursors to each other in vivo. Using site‐directed and deletion mutagenesis of the P/C gene cloned in SP6 and in vitro translation of the mRNAs, we show that the 5′ most proximal initiation codon of the mRNA is an ACG at position 81, responsible for C’ synthesis. The succeeding initiation codons, all ATGs, are responsible for the P protein (position 104), the C protein (position 114) and the Y protein(s) (either positions 183 or 201). Examination of the relative molar amounts of the C′, P and C proteins found in vivo suggests that an ACG in an otherwise favorable context is almost as efficient for ribosome initiation as an ATG in a less favored context, but only 10‐20% as efficient as an ATG in a more favored context. The judicious choice of increasingly more favorable initiation codons in the P/C gene allows multiple proteins to be made from a single mRNA.
Flicker Emission of Wind Turbines Caused by Switching Operations

This paper presents the modeling and analysis of the flicker of wind turbines. Special emphasis is on explaining the start-up procedure and deriving equations for the calculation of flicker produced by switching operations. The derived equations are compared with intemational standards. The paper includes measurements of the start and stop of different types of turbines. Finally, the paper makes a comparison of flicker limitations at wind parks.
Hemostatic Risk Factors and Arterial Thrombotic Disease

Summary The pathogenesis of arterial thrombotic disease involves multiple genetic and environmental factors related to atherosclerosis and thrombosis. Acute thrombosis at the site of a ruptured, lipid-rich atherosclerotic plaque is the usual precipitating event in the transition from stable or subclinical atherosclerotic disease to acute myocardial infarction (MI), stroke, or peripheral arterial occlusion (1). Pathologic studies of coronary arteries in acute MI suggest that the acute thrombosis likely involves activation of both platelets and the coagulation system.
Cation channels in basolateral membranes of sheep parotid secretory cells.

We observed 240-pS K+ channels in 63% of cell-attached patches, and 30-pS K+ channels were observed in 95% of cell-attached patches. The 240-pS K+ channel had the relative permeability sequence of K+ (1) = Rb+ (1) > Cs+ (0.3) >> Na+ (0.03) and the relative conductance sequence of K+ (1) > Rb+ (0.22) > Cs+ (0.05) > Na+ (0). It was activated by intracellular free Ca2+ and by depolarization. It was blocked by 10 mmol/l tetraethylammonium (TEA) applied extracellularly. The 30-pS K+ channel had the relative permeability sequence of K+ (1) = Rb+ (1) > Cs+ (> Na+ (< 0.09) and the relative conductance sequence of K+ (1) > Rb+ (0.45) > Cs+ (0) = Na+ (0). Its activity was not sensitive to cytosolic free Ca2+ or membrane potential, and it was not blocked by 10 mmol/l TEA extracellularly. Acetylcholine (10 mumol/l) activated the 240-pS voltage-activated and Ca(2+)-activated K+ channels but did not activate the 30-pS K+ channels. We conclude that the 30-pS K+ channel probably determines the properties of the basolateral membrane in unstimulated sheep parotid secretory cells, whereas the 240-pS voltage-activated and Ca(2+)-activated K+ channel may be important during parasympathomimetic stimulation.
African American Stepfather–Stepchild Relationships, Marital Quality, and Mental Health

ABSTRACT This study examined associations between stepfather–stepchild relationship quality, stepfathers’ depressive symptoms, and two aspects of marriage: marital quality and positive marital interactions. Marital quality was assessed in terms of commitment, trust, passionate and friendship-based love, and happiness. Marital interactions were assessed in terms of intimacy, shared activities, and verbal communication. Using data collected from 149 recently married African American stepfathers, structural equation modeling revealed that when stepfathers reported more positive relationships with their stepchildren, they also reported more positive marital quality and a higher frequency of positive marital interactions (relationship-solidifying activities), and that, in turn, was associated with stepfathers experiencing fewer depressive symptoms.
Cloning, Expression, Characterization, and Biocatalytic Investigation of the 4-Hydroxyacetophenone Monooxygenase from Pseudomonas putida JD1

ABSTRACT While the number of available recombinant Baeyer-Villiger monooxygenases (BVMOs) has grown significantly over the last few years, there is still the demand for other BVMOs to expand the biocatalytic diversity. Most BVMOs that have been described are dedicated to convert efficiently cyclohexanone and related cyclic aliphatic ketones. To cover a broader range of substrate types and enantio- and/or regioselectivities, new BVMOs have to be discovered. The gene encoding a BVMO identified in Pseudomonas putida JD1 converting aromatic ketones (HAPMO; 4-hydroxyacetophenone monooxygenase) was amplified from genomic DNA using SiteFinding-PCR, cloned, and functionally expressed in Escherichia coli. Furthermore, four other open reading frames could be identified clustered around this HAPMO. It has been suggested that these proteins, including the HAPMO, might be involved in the degradation of 4-hydroxyacetophenone. Substrate specificity studies revealed that a large variety of other arylaliphatic ketones are also converted via Baeyer-Villiger oxidation into the corresponding esters, with preferences for para-substitutions at the aromatic ring. In addition, oxidation of aldehydes and some heteroaromatic compounds was observed. Cycloketones and open-chain ketones were not or poorly accepted, respectively. It was also found that this enzyme oxidizes aromatic ketones such as 3-phenyl-2-butanone with excellent enantioselectivity (E ≫100).
An Analytical Model of Electric Substrate Losses for Planar Spiral Inductors on Silicon

This paper presents a physically based model for estimating the substrate losses due to electric field penetration for planar spiral inductors on silicon not using patterned ground shield. The model, which does not use any fitting parameter, shows excellent agreement with measured data. It has been tested across a variety of inductor geometries and two different substrates up to 10 GHz
INVESTOR REACTION TO MUTUAL FUND PERFORMANCE: EVIDENCE FROM UK DISTRIBUTION CHANNELS

Existing work on the flow–performance relation in mutual funds focuses on the average U.S. investor, obscuring the contributions of different clienteles. We analyze UK data on monthly fund sales and purchases made via seven distinct distribution channels. We show that there exist marked differences in the reaction to fund performance between different types of retail and institutional investors. These differences can be understood by considering the incentives of parties involved in each channel. Our analysis indicates that the well-documented aggregate net flow–performance convexity in mutual funds is driven by the extreme reaction of retail inflows to favorable performance, particularly from independently advised investors.
L'évaluation: un élément clef du dispositif d'accompagnement des projets incubés ?

Aujourd'hui en France, on compte trente incubateurs publics qui coordonnent leurs actions au sein de France Incubation. Notre etude est realisee aupres de l'incubateur public Incuballiance issu de la fusion en janvier 2005, de l'IFSI (Ile de France Sud Incubation) et de l'IDFI (Ile De France Incubation). Cette recherche s'interesse a la problematique de l'apprentissage managerial des porteurs de projet de creation d'entreprise innovante au sein d'une structure d'accompagnement : un incubateur public. Elle vise principalement la mise en evidence des logiques d'accompagnement pour favoriser cet apprentissage, au sein de la structure d'incubation. Le coeur de notre questionnement est donc : comment le porteur de projet apprend t-il, et en quelles occasions ? Pour repondre a cette question nous developperons dans une premiere partie les enjeux theoriques de la recherche, en definissant les concepts centraux que nous mobilisons : les liens operes dans la litterature entre le processus d'apprentissage et l'innovation (dans ses acceptions technique et manageriale), la relation d'accompagnement dans la demarche entrepreneuriale, puis l'importance de l'evaluation dans les dispositifs d'apprentissage. Dans une deuxieme partie nous presentons notre terrain d'etude Incuballiance, en explicitant les dispositifs multiples de l'accompagnement, puis en mettant l'accent sur le caractere systemique des relations qu'un porteur de projet peut nouer a partir de l'incubateur. Enfin, dans une troisieme partie nous montrons comme l'evaluation est l'element cle du dispositif d'accompagnement. En effet, elle apparait comme un outil de pilotage des projets par la structure formelle d'incubation et elle oriente en grande partie la nature des apprentissages produits par les porteurs de projet.
Cognitive Tunneling: Use of Visual Information under Stress

References to “tunnel vision” under stress are considered to describe a process of attentional, rather than visual, narrowing. The hypothesis of Easterbrook that the range of cue utilization is reduced under stress was tested with a primary task located in the visual periphery. High school volunteers performed a visual discrimination task with choice reaction time (RT) as the dependent variable. A 2 × 3 order of presentation by practice design, with repeated measures on the last factor, was employed. Two levels of stress, high and low, were operationalized by the subject's performing in the presence of an evaluative audience or alone. Pulse rate was employed as a manipulation check on arousal. The results partially supported the hypothesis that a peripherally visual primary task could be attended to under stress without decrement in performance.
Aprendizajes constructivistas y no constructivistas : una diferenciación obligada para nuestras aulas

En condiciones como las actuales, caracterizadas por la emergencia de una sociedad digitalizada, donde se imponen renovados ordenes psicosociologicos y ante el poder mass-mediatico, resulta apremiante ofrecer un analisis sobre los signos y sintomas de la aplicacion de las nuevas tecnologias al aula. Se constata la sucesion de cambios en el plano comunicativo, asi como en los discursos y practicas, si bien permanece la esencia de la vision iluminista de la educacion, a pesar del intento de remozar su apariencia a traves de la vision iluminista de la educacion, a pesar del intento de remozar su apariencia a traves de la introduccion de tales redes interactivas. En este articulo se ofrece una diagnosis de la escuela y la sociedad contemporanea, constatandose la retroalimentacion de sus crisis. Se propone que el aula puede convertirse en agora electronica, si bien se analizan los efectos perversos derivados de tal aplicacion.
Multitrophic interactions below and above ground: en route to the next level

1. Plants mediate multiple interactions between below‐ground (BG) and above‐ground (AG) heterotrophic communities that have no direct physical contact. These interactions can be positive or negative from the perspective of each player, can go from the BG to the AG community or vice versa, and comprise representatives of different phyla. Here we highlight emerging general patterns and discuss future research directions.
Angular momentum in turns and abrupt starts : strategies for bipedal balance control

Transients occur in human walking during a transition to, from, and between steady state walking and act as an impulse destabilizing an otherwise normal gait cycle. Turns and accelerated starts are all common transients encountered and managed intelligently by humans everyday. The population of elderly has increased and understanding balance control in healthy subjects will be more important. In addition, humanoid bipeds are rapidly becoming a more common part of our everyday life. Therefore, they must also be able to navigate our environments adroitly if they are to assist us in our daily living. This thesis takes biomechanical principals of angular momentum and applies them to healthy subjects in an effort to elucidate human balance control strategies. Each transient task is unique, and despite large segmental contributions to whole-body angular momentum during movement, the whole-body angular momentum remains tightly regulated. A analysis of segmental contributions to the principal components explaining more than 90% makes clear the balance control strategy used by healthy humans during these transients. Advisor: Professor Hugh Herr Associate Professor of Media Arts and Sciences (Signature of Advisor) Reader One: Professor Joeseph Paradiso Professor of Media Arts and Sciences (Signature of Reader) Reader Two: Professor Russell Tedrake Assistant Professor of Electrical Engineering and Computer Science (Signature of Reader)
Can you recognize a patient at risk for a hypercoagulable state?

&NA; EARN CATEGORY I CME CREDIT by reading this article and the article beginning on page 39 and successfully completing the posttest on page 44. Successful completion is defined as a cumulative score of at least 70% correct. This material has been reviewed and is approved for 1 hour of clinical Category I (Preapproved) CME credit by the AAPA. The term of approval is for 1 year from the publication date of December 2008. Describe the elements of the clotting system Review the workup of excessive clotting, with special focus on laboratory evaluation Outline the differential diagnosis of hypercoagulable states using “5 Ps Had Caused Clots” Know the treatment of hypercoagulability, including lifestyle modifications and medications A disrupted clotting system can lead to life‐threatening thrombosis. PAs can use a simple mnemonic to remember the differential diagnosis for hypercoagulable states.
STUDIES OF SMALL INTESTINE DURING DEVELOPMENT. III. INFANTILE DIARRHEA ASSOCIATED WITH INTOLERANCE TO DISACCHARIDES.

6 infant aged from 5 weeks to 12 months who demonstrated chronic fermentative diarrhea vomiting excessive flatus poor weight gain and intolerance to lactose and sucrose were observed for the purpose of this study. After the sugars to which they were intolerant were removed from their diet the infants began to gain weight and diarrhea disappeared. They were reexamined after a period of time and found to respond normally to lactose and sucrose. It is interesting to note that the clinical symptoms of these patients whose intolerance to disaccharides was acquired were identical to those of patients with congenital absence of intestinal disaccharides.
HERBÁRIO VIRTUAL DA FLORA E DOS FUNGOS A. DE SAINT-HILAIRE

O "Herbario Virtual A. de Saint-Hilaire" reune toda a colecao botânica de Augustin Francois-Cesar Prouvencal de Saint-Hilaire (1799-1853), naturalista frances que visitou o Brasil entre 1819 e 1822. O Herbario Virtual e composto pelas informacoes bibliograficas, notas de campo, mapas e ilustracoes representativas da colecao, todas relacionadas com os respectivos especimes depositados em herbario. Toda informacao foi reunida num sistema de informacao que esta disponivel na internet, no endereco hvsh.cria. org.br, para consulta livre e esta aberto a contribuicoes de especialistas. A analise continua dos dados qualificam as informacoes disponiveis no herbario virtual. A difusao do conhecimento se da atraves de publicacoes cientificas e programas de oficinas tecnicas e educacionais das instituicoes parceiras. Ressalte-se ainda a importância desta experiencia piloto de repatriamento de dados cientificos e historicos que inspirou outras iniciativas no Brasil, como por exemplo o programa REFLORA do CNPq. Palavras chave: Herbario historico, Colecoes Cientificas, Repatriacao
c-Met Must Translocate to the Nucleus to Initiate Calcium Signals*♦

Hepatocyte growth factor (HGF) is important for cell proliferation, differentiation, and related activities. HGF acts through its receptor c-Met, which activates downstream signaling pathways. HGF binds to c-Met at the plasma membrane, where it is generally believed that c-Met signaling is initiated. Here we report that c-Met rapidly translocates to the nucleus upon stimulation with HGF. Ca2+ signals that are induced by HGF result from phosphatidylinositol 4,5-bisphosphate hydrolysis and inositol 1,4,5-trisphosphate formation within the nucleus rather than within the cytoplasm. Translocation of c-Met to the nucleus depends upon the adaptor protein Gab1 and importin β1, and formation of Ca2+ signals in turn depends upon this translocation. HGF may exert its particular effects on cells because it bypasses signaling pathways in the cytoplasm to directly activate signaling pathways in the nucleus.
Progression of regulatory gene expression states in fetal and adult pro‐T‐cell development

Summary: Precursors entering the T‐cell developmental pathway traverse a progression of states characterized by distinctive patterns of gene expression. Of particular interest are regulatory genes, which ultimately control the dwell time of cells in each state and establish the mechanisms that propel them forward to subsequent states. Under particular genetic and developmental circumstances, the transitions between these states occur with different timing, and environmental feedbacks may shift the steady‐state accumulations of cells in each state. The fetal transit through pro‐T‐cell stages is faster than in the adult and subject to somewhat different genetic requirements. To explore causes of such variation, this review presents previously unpublished data on differentiation gene activation in pro‐T cells of pre‐T‐cell receptor‐deficient mutant mice and a quantitative comparison of the profiles of transcription factor gene expression in pro‐T‐cell subsets of fetal and adult wildtype mice. Against a background of consistent gene expression, several regulatory genes show marked differences between fetal and adult expression profiles, including those encoding two basic helix‐loop‐helix antagonist Id factors, the Ets family factor SpiB and the Notch target gene Deltex1. The results also reveal global differences in regulatory alterations triggered by the first T‐cell receptor‐dependent selection events in fetal and adult thymopoiesis.
The contrasting role of higher order awareness in hypnosis and meditation

Meditation and hypnosis might be regarded as involving similar processes and skills. For example, both meditation and hypnosis are associated with high levels of absorption and imaginative capacity, and both can be used for self-regulation. Research has also shown that meditation improves attentional functioning, and that hypnotic response can involve attentional strategies. However, we argue that hypnosis and mindfulness meditation are essentially different. Crucially, hypnotic experience results from a lack of awareness of mental states (specifically, of intentions); by contrast, mindfulness meditation aims to develop accurate meta-awareness. Hypnosis is a form of self deception; meditation a way of getting to know your mind. We discuss the empirical relation of both meditation and hypnosis to higher order awareness of mental states, and suggest further research.