Miracle-dz/UniFAR-FTUs huggingface dataset:
Association between social media use (Twitter, Instagram, Facebook) and depressive symptoms: Are Twitter users at higher risk?

Background: The purpose of this study was to determine the association between social media dependence and depressive symptoms and also, to characterize the level of dependence. It was a transversal, analytical research. Subjects and Methods: The stratified sample was 212 students from a private university that used Facebook, Instagram and/or Twitter. To measure depressive symptoms, Beck Depression Inventory was used, and to measure the dependence to social media, the Social Media Addiction Test was used, adapted from the Internet Addiction Test of Echeburúa. The collected data were subjected for analysis by descriptive statistics where STATA12 was used. Results: The results show that there is an association between social media dependence and depressive symptoms (PR [Prevalence Ratio] = 2.87, CI [Confidence Interval] 2.03–4.07). It was also shown that preferring the use of Twitter (PR = 1.84, CI 1.21–2.82) over Instagram (PR = 1.61, CI 1.13–2.28) is associated with depressive symptoms when compared to the use of Facebook. Conclusion: Excessive social media use is associated with depressive symptoms in university students, being more prominent in those who prefer the use of Twitter over Facebook and Instagram.
Leukemia therapy by flavonoids: Future and involved mechanisms

Flavonoids are a varied family of phytonutrients (plant chemicals) usually are detected in fruits and vegetables. In this big family, there exist more than 10,000 members that is separated into six chief subtypes: isoflavonols, flavonoenes, flavones, flavonols, anthocyanins, and chalcones. The natural compounds, such as fruits, have visible positive effects in regulating of survival involved signaling pathways that performance as the regulator of cell survival, growth, and proliferation. Researchers have established that commonly consumption up flavonoids decreases incidence and development risk of certain cancers, especially leukemia. Flavonoids have been able to induce apoptosis and stimulate cell cycle arrest in cancer cells via different pathways. Similarly, they have antiangiogenesis and antimetastasis capability, which were shown in wide ranges of cancer cells, particularly, leukemia. It seems that flavonoid because of their widespread approval, evident safety and low rate of side effects, have hopeful anticarcinogenic potential for leukemia therapy. Based on the last decade reports, the most important acting mechanisms of these natural compounds in leukemia cells are stimulating of apoptosis pathways by upregulation of caspase 3, 8, 9 and poly ADP‐ribose polymerase (PARP) and proapoptotic proteins, particularly Bax activation. As well, they can induce cell cycle arrest in target cells not only via increasing of activated levels of p21 and p53 but also by inhibition of cyclins and cyclin‐dependent kinases. Furthermore, attenuation of neclear factor‐κB and signal transducer and activator of transcription 3 activation, suppression of signaling pathway and downregulation of intracellular antiapoptotic proteins are other significant antileukemic function mechanism of flavonoids. Overall, it appears that flavonoids are promising and effective compounds in the field of leukemia therapy. In this review, we tried to accumulate and revise most promising flavonoids and finally declared their major working mechanisms in leukemia cells.
Micropulse Laser for Persistent Sub-Retinal Fluid in a Patient Previously Treated for Rhegmatogenous Retinal Detachment

The purpose of this study was to report the resolution of persistent Sub-Retinal Fluid (SRF) induced by subthreshold micropulse laser treatment in a patient, formerly treated for rhegmatogenous retinal detachment by retinal pneumopexy. The case was a 41-year-old male, who initially presented macula-splitting rhegmatogenous retinal detachment and corrected distance visual acuity of 20/40 in his left eye. He was treated by retinal pneumopexy and laser retinopexy. Retina was flattened and vision improved to 20/30. However, the subretinal fluid (SRF) under the fovea was persistently observed on repeated retinal exams. Fourteen months after the initial pneumopexy, subthreshold micropulse laser was applied to cover the entire area of the SRF. The improvement started two weeks afterwards and the SRF completely resolved within four months after the application of micropulse laser. Corrected distance visual acuity improved from 20/30 to 20/20, accompanied by marked improvement in patient’s complaints on visual blurriness. The patient was followed up for three years and no recurrence of SRF was noted. The findings of this report indicate that subthreshold micropulse laser may serve as a therapeutic option for persistent SRF, which may be observed after successful retinal detachment repair.
Delivering information literacy within a global health care degree: reflections from a health information specialist.

Higher education institutions (HEIs) have a growing interest in establishing International joint degree programmes in developing countries. A Master's degree in global health care is an international joint degree programme run by two Finnish Universities of Applied Sciences and one Kenyan University. To provide students and staff members who live in Finland with an authentic experience of the circumstances in the developing countries, part of the programme involves delivering a two-week intensive course in Kenya. Health library services and information literacy training have a significant presence during this intensive course. In this paper, guest writer Marketta Fredriksson describes and reflects on the involvement of the health care library team in the development and implementation of this degree and delivery of the intensive course in Kenya.H. S.
SMRT long reads and Direct Label and Stain optical maps allow the generation of a high-quality genome assembly for the European barn swallow (Hirundo rustica rustica)

Background The barn swallow (Hirundo rustica) is a migratory bird that has been the focus of a large number of ecological, behavioural and genetic studies. To facilitate further population genetics and genomic studies, here we present a reference genome assembly for the European subspecies (H. r. rustica). Findings As part of the Genome10K (G10K) effort on generating high quality vertebrate genomes, we have assembled a highly contiguous genome assembly using Single Molecule Real-Time (SMRT) DNA sequencing and several Bionano optical map technologies. We compared and integrated optical maps derived both from the Nick, Label, Repair and Stain and from the Direct Label and Stain (DLS) technologies. As proposed by Bionano, the DLS more than doubled the scaffold N50 with respect to the nickase. The dual enzyme hybrid scaffold led to a further marginal increase in scaffold N50 and an overall increase of confidence in the scaffolds. After removal of haplotigs, the final assembly is approximately 1.21 Gbp in size, with a scaffold N50 value of over 25.95 Mbp. Conclusions This high-quality genome assembly represents a valuable resource for further studies of population genetics and genomics in the barn swallow, and for studies concerning the evolution of avian genomes. It also represents one of the very first genomes assembled by combining SMRT long-read sequencing with the new Bionano DLS technology for scaffolding. The quality of this assembly demonstrates the potential of this methodology to substantially increase the contiguity of genome assemblies.
Age and sex‐related differences in performance, carcass traits, hemato‐biochemical parameters, and meat quality in Japanese quails

ABSTRACT The effect of sex and age of Japanese quails (Coturnix japonica) on their performance, blood biochemical parameters, carcass traits, and meat quality was evaluated in this experiment. A total of 450, 3‐wk‐old Japanese quails (225 males and 225 females) were equally divided into six groups (75 birds each) in a 2 × 3 factorial design, including two sex (male and female) and three slaughter ages (5, 6, and 7 wk of age). Each group was subdivided into five replicates each of 15 birds. The body weight (BW) and feed intake (FI) values were significantly higher in female quails than in males, and these values showed an increasing trend with age. Female quails recorded the higher percentages of liver and giblets, and lower heart percentage than males. Sex × age interaction had a significant impact on carcass parameters except for breast and thigh yield. No significant sex‐based differences were detected for quail meat chemical composition except for meat fat content. Moisture and ash content of meat were gradually deceased with age. Meat from female showed higher values for tenderness, intramuscular fat percentage (IMF), and meat lightness, but showed lower values of water holding capacity (WHC) and meat redness. Meat juiciness and tenderness decreased, while WHC, IMF, and meat redness increased with age. Mean values of red blood cells (RBC), packed cell volume (PCV), hemoglobin (Hb), and white blood cells (WBC) were higher (P < 0.05) in male quails than female ones. Plasma total protein, cholesterol, triglycerides, calcium, phosphorus, and uric acid were decreased in males comparable to females. The progress in quail age (5 to 7 wk) leads to significant increase in values of RBC, PCV, and Hb. Plasma cholesterol, total protein, triglycerides, and hemoglobin were linearly deceased with age. It could be concluded that Japanese quails (both males and females) should be slaughtered at 5 or 6 wk of age to obtain higher carcass yields and the best meat quality and composition. Furthermore, male quails recorded the highest values of meat quality and composition when compared to females.
Acute myeloid leukemia remodels endosteal vascular niche into a leukemic niche.

At steady state, normal hematopoiesis occurs in highly specialized bone marrow (BM) niches that regulate self-renewal, quiescence and differentiation of hematopoietic stem cells (HSCs) to maintain continuous supply of mature cells throughout the lifespan (1,2). Ironically, the BM niche is also the site for malignant hematopoiesis. Leukemia cells, akin to the invasive weed Parthenium hysterophorus that destroyed the habitat of native plant & animal species (3), are ruthless competitors and destroy the natural habitat of normal hematopoietic stem and progenitor cells by altering the behavior of supporting cells in the BM niche (4-6). Apart from leukemias, defects in hematopoiesis are frequently observed in different cancer patients with BM metastasis (7,8).
Zidovudine-induced lactic acidosis with acute pancreatitis and myopathy: Lethal and rare complications

Highly active antiretroviral therapy (HAART) is nowadays universally available to patients with HIV/AIDS. This has led to increased longevity in people living with HIV/AIDS. However, these patients frequently face chronic and rarely acute life-threatening complications of HAART. Herein, we report the case of a patient who was on HAART and developed zidovudine-induced lactic acidosis, acute pancreatitis, and myopathy. Although these acute complications are rare, a high index of suspicion is required for early diagnosis and to reduce significant morbidity and mortality.
Simulation of inventory Policy for Product with Price and Time-dependent demand for deteriorating Item

In a declining market for goods, we optimize the net profit in business when inventory management allows change in the selling prices n times over time horizon. We are computing optimal number of changes in prices, respective optimal prices, and optimal profit in each of the cycle for a deteriorating product. This paper theoretically proves that for any business setup there exists an optimal number of price settings for obtaining maximum profit. Theoretical results are supported by numerical examples for different setups (data set) and it is found that for every setup the dynamic pricing policy outperforms the static pricing policy. In our model, the deterioration factor has been taken into consideration. The deteriorated units are determined by the recurrence method. Also we studied the effect of different parameters on optimal policy with simulation. For managerial purposes, we have provided some "suggested intervals" for choosing parameters depending upon initial demand, which help to predict the best prices and arrival of customers (demand).
Martelella caricis sp. nov., isolated from a rhizosphere mudflat.

A novel marine bacterium, designated GH2-8T, was isolated from a rhizosphere mudflat of a halophyte (Carexscabrifolia) in Gangwha Island, Republic of Korea and its taxonomic status was investigated by a polyphasic approach. Cells of strain GH2-8T were Gram-stain-negative, strictly aerobic, oxidase-positive, catalase-positive and non-motile rods that showed growth at 10-30 °C, pH 5-10 and 0-11 % (w/v) NaCl. The predominant respiratory quinone was Q-10. The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid and two unidentified lipids. The major fatty acids were summed feature 8, C16 : 0, C19 : 0cyclo ω8c, C18 : 1ω7c 11-methyl and summed feature 2. The G+C content of the genomic DNA was 53.4 mol%. Comparative analysis of the 16S rRNA gene sequences revealed that the organism belonged to the order 'Rhizobiales' and formed a distinct subline at the root of radiation encompassing members of the genus Martelella. The 16S rRNA gene sequence similarities to the phylogenetic neighbours were Martelella mediterranea (97.1 %), Martelella suaedae (96.9 %), Martelella endophytica (96.6 %), Martelella limonii (96.3 %), Martelella mangrovi (96.1 %) and Martelella radicis (95.5 %). Strain GH2-8T showed low 16S rRNA gene sequence similarities (<93.8 %) to other representatives of the order 'Rhizobiales'. On the basis of the results of phenotypic and phylogenetic analyses, strain GH2-8T (=KACC 19402T=KCCM 90275T=KCTC 62126T=NBRC 113213T) is considered to represent a novel species of the genus Martelella for which the name Martelellacaricis sp. nov. is proposed.
The mechanosensitive Piezo1 channel: a three‐bladed propeller‐like structure and a lever‐like mechanogating mechanism

The evolutionarily conserved Piezo proteins, including Piezo1 and Piezo2, constitute a bona fide class of mechanosensitive (MS) cation channels, which play critical roles in various mammalian physiologies, including sensation of touch, proprioception and regulation of vascular development, and blood pressure. Furthermore, mutations in Piezos have been linked to various human genetic diseases, validating their potential as therapeutic targets. Thus, it is pivotal to understand how Piezo channels effectively convert mechanical force into selective cation permeation, and therefore precisely control the various mechanotransduction processes. On the basis of our recently determined cryoelectron microscopy structures of the full‐length 2547‐residue mouse Piezo1, structure‐guided mutagenesis, and electrophysiological and pharmacological characterizations, here we focus on reviewing the key structural features and functional components that enable Piezo1 to employ a lever‐like mechanogating mechanism to function as a sophisticated mechanotransduction channel.
Architectural roles of Cren7 in folding crenarchaeal chromatin filament

Archaea have evolved various strategies in chromosomal organization. While histone homologues exist in most archaeal phyla, Cren7 is a chromatin protein conserved in the Crenarchaeota. Here, we show that Cren7 preferentially binds DNA with AT‐rich sequences over that with GC‐rich sequences with a binding size of 6~7 bp. Structural studies of Cren7 in complex with either an 18‐bp or a 20‐bp double‐stranded DNA fragment reveal that Cren7 binds to the minor groove of DNA as monomers in a head‐to‐tail manner. The neighboring Cren7 monomers are located on the opposite sides of the DNA duplex, with each introducing a single‐step sharp kink by intercalation of the hydrophobic side chain of Leu28, bending the DNA into an S‐shape conformation. A structural model for the chromatin fiber folded by Cren7 was established and verified by the analysis of cross‐linked Cren7‐DNA complexes by atomic force microscopy. Our results suggest that Cren7 differs significantly from Sul7, another chromatin protein conserved among Sulfolobus species, in both DNA binding and deformation. These data shed significant light on the strategy of chromosomal DNA organization in crenarchaea.
Equine bone marrow-derived mesenchymal stem cells: optimization of cell density in primary culture.

Background The primary cell seeding density of bone marrow-derived mononuclear cells (BM-MNCs) affects several cellular behaviors, including attachment to the culture dish, proliferation, and differentiation. Methods The aim of this study was to determine the best density of equine BM-MNCs in primary culture (P0) for obtaining the maximum bone marrow-derived mesenchymal stem cell (BM-MSC) yields at the end of P0. Bone marrow samples of two healthy mares were aspirated. The MNCs were isolated and cultured at different densities (1×105, 2×105, 4×105, 8×105, and 1×106 cells/cm2). Within the 7th and 14th days after seeding, the colonies containing more than 15 cells were counted and the percentage of confluency and the number of cells were calculated on day 21. Results The lowest density of MNCs was associated with the least number of colonies, number of adherent cells, and confluency percentage, whereas the highest density was associated with the maximum number of colonies and confluency percentage (P<0.05). However, the maximum number of cells at the end of P0 was associated with the intermediate (4×105 cells/cm2) and the highest concentration (P<0.05). Conclusions The maximum number of MSCs at the end of P0 was obtained at the densities of 1×106 and, especially, at 4×105 cells/cm2.
Emergence of Enterobacteriaceae co-producing CTX-M-15, ArmA and PMQR in Poland.

BACKGROUND Plasmid-mediated extended-spectrum β-lactamases (ESBLs), 16S rRNA methylases and quinolone resistance mechanisms (PMQRs) are well-known agents conferring resistance to more than 1 antimicrobial in its group. The accumulation of these agents poses, therefore, a serious risk to public health. OBJECTIVES The objective of this study was to investigate the presence of common ß-lactamases and 16S rRNA methylases in Qnr-producing Enterobacteriaceae and their genetic relatedness. MATERIAL AND METHODS We examined 18 Qnr-producing isolates (Klebsiella pneumoniae n = 8, Enterobacter cloacae n = 6 and Escherichia coli n = 4) selected from a collection of 215 ciprofloxacin-resistant strains obtained from patients in a 1030-bed tertiary hospital from 1 March to 31 August 2010. The antibiotics minimum inhibitory concentration (MIC) was determined by E-test. The detection of common ß-lactamases, 16S rRNA methyltransferases and PMQR genes was performed by polymerase chain reaction (PCR) and sequencing. Genetic relatedness was assessed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). RESULTS All the isolates tested were susceptible to carbapenems and colistin, while 16 were multidrug-resistant. Thirteen, 2 and 2 isolates carried qnrB1, qnrA1 and qnrS1, respectively. Ten of 13 qnrB1-positive Enterobacteriaceae also carried genes encoding for aac(6')-Ib-cr and at least 1 ESBL. The blaCTX-M-15 gene was the most common ESBL. The most prevalent combination of genes was qnrB1+aac(6')-Ib-cr+blaTEM-1+blaCTX-M-15. Two isolates of K. pneumoniae and E. cloacae were found to bear multiple extended range resistance traits: ArmA, CTX-M-15, QnrB1, and AAC (6')-Ib-cr. The PFGE showed that most of the isolates exhibited individual DNA patterns, whilst MLST assigned K. pneumoniae (n = 8) to 5 sequence types (STs) (ST15, ST323, ST336, ST147, and ST525), E. coli (n = 4) to 2 (ST131 and ST1431) and E. cloacae (n = 5) to 4 (ST90, ST89, ST133, and the novel ST407). CONCLUSIONS Our findings reveal the accumulation of resistance traits and their important role in spreading of multiresistant bacteria among hospitalized patients.
Pharmacological Inhibition of Pten Restores Remote Ischemic Postconditioning Cardioprotection in Hypercholesterolemic Mice: Potential Role of PTEN/AKT/GSK3β Signals.

Although remote ischemic postconditioning (RIPC) was shown to confer cardioprotection against myocardial ischemia/reperfusion (I/R) injury in normal animals, whether RIPC-induced cardioprotection are altered in the presence of hypercholesterolemia, a comorbidity with acute myocardial infarction (AMI) patients, has yet to be determined. Normal or 2% cholesterol chow was fed to male C57BL/6J mice for 12 weeks to induce hypercholesterolemia, then normal or hypercholesterolemic murine hearts were exposed to AMI by coronary artery ligation. RIPC was induced by four episodes of 5 min femoral artery occlusion followed by 5 min reperfusion immediately after myocardial reperfusion in mice. Following I/R, RIPC significantly attenuated post-ischemic infarct size, hindered cardiomyocyte apoptosis, improved cardiac systolic function, decreased PTEN expression and further increased Akt and GSK-3β phosphorylation in non-hypercholesterolemic, but not in hypercholesterolemic mice. Application of the PTEN inhibitor BpV (bisperoxovanadium) (1.0 mg/kg) reduced post-ischemic infarct size, attenuated cardiomyocyte apoptosis and improved cardiac dysfunction in normal, but not in hypercholesterolemic mice. Further, increased dose of BpV (2 mg/kg or 10 mg/kg) failed to rescue the detrimental effects of hypercholesterolemia on I/R in mice following I/R. Especially important, we demonstrated that the combination BpV and RIPC exerted marked cardioprotective effects both in normal and hypercholesterolemic mice with I/R, indicating that PTEN inhibition restores RIPC-elicited myocardial protection in the presence of hypercholesterolemia. Our results demonstrated that hypercholesterolemia attenuated RIPC-induced cardioprotection against I/R injury by alteration of PTEN/Akt/GSK3β signals, and inhibition of PTEN rescued RIPC-induced cardioprotection in the presence of hypercholesterolemia.
Mutant p53 in colon cancer

Abstract The accumulation of genetic alterations in driver genes is responsible for the development and malignant progression of colorectal cancer. Comprehensive genome analyses have revealed the driver genes, including APC, KRAS, TGFBR2, and TP53, whose mutations are frequently found in human colorectal cancers. Among them, the p53 mutation is found in ~60% of colorectal cancers, and a majority of mutations are missense-type at ‘hot spots’, suggesting an oncogenic role of mutant p53 by ‘gain-of-function’ mechanisms. Mouse model studies have shown that one of these missense-type mutations, p53 R270H (corresponding to human R273H), causes submucosal invasion of intestinal tumors, while the loss of wild-type p53 has a limited effect on the invasion process. Furthermore, the same mutant p53 promotes metastasis when combined with Kras activation and TGF-β suppression. Importantly, either missense-type p53 mutation or loss of wild-type p53 induces NF-κB activation by a variety of mechanisms, such as increasing promoter accessibility by chromatin remodeling, which may contribute to progression to epithelial–mesenchymal transition. These results indicate that missense-type p53 mutations together with loss of wild-type p53 accelerate the late stage of colorectal cancer progression through the activation of both oncogenic and inflammatory pathways. Accordingly, the suppression of the mutant p53 function via the inhibition of nuclear accumulation is expected to be an effective strategy against malignant progression of colorectal cancer.