Miracle-dz/UniFAR-FTUs huggingface dataset:
RAT OVARIAN INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN-3: A GROWTH HORMONE-

To further the identification and chanicterization of insulin-llke growth factor binding proteins at the level of the immature rat ovary. we have et out to study the ovarian expression, cellular localization, and hormonal regulation of the insulin-like growth factor binding protein (IGFBP)-3. To this end. use was made of a solution hybridization/RNAse protection assay wherein ovarian total RNA from immature (21-23 days old) female rats was hybridized with a 343 bases-long I'? -labeled r;it IGFBP-3 riboprobe. As in liver, a single protected fragment (315 b;lses-long) corresponding to IGFBP-3 transcripts was Identified in whole ovarian material. Cellular Iocahzation sludirs revealed the IGFBP-3 gene lo be exclu\lvely expressed in the theta-lnterstltlal mther than the granuloaa cell compartment. To confirm presence and celluku distribution of the IGFBP-3 protein, media conditloned by cultured granulosa cells, theta-interstitial cells. and whole ovarian dispersates were subjected to Western Ligand Blotting. Importantly,, media conditioned by cultured theta-interstitial (but not granuloea) cells displayed an IGFBP the size of rat IGFBP-3 (46kDa) a! , detemimed by comigration with a rot serum standard. A aimilarly- sized band was apparent in media condinoned by cultured whole ovarian dispersates reflecting in all likelihood the conmbutmn of the theca- interstitial cell component. S~gniticantly. deglycosylation of media conditioned by cultured theta-interstitial cell, revealed the glycosylated nature of the 4hkDa IGFBP species ar Judged by the appaxnt reduction In its molecular size to 35kDa. Similar alterations were noted in corresponding rat serum samples. Hypophysectomy of immature rats resulted in a modest but statistically insigmiicant decrease in the relative (densttometrically-quantilied) abundance of ovarian IGFBP-3 transcripts. an effect further augmented by the systemic provisIon of either FSH or diethylstilbestrol (DES). In conuast. systemic treatment of hypophysectomized rats with GH produced a marked (3.2.fold) increase (P&OS) in the steady state levels of ovarian (as well as hepatic) IGFBP-3 transcripts. However, the concurrent provision of either FSH or DES resulted in substantial (P<O.OS) attenuation (7X and 57% inhibltion, respectively) of the upregulatory GH effect. These findings document the highly compartmentalized expression of the IGFBP-3 gene at the level of the immature rtt ovary, imphcate the theta-interstitial cell as the sole source of its generation, reveal its pituitary dependence, and disclose its d~amenically-opposed (indeed antagonistic) regulation by FSH (or estrogens) and GH. These observations mise the possibility that hypersomatotropic-induced reproductive dysfunction may be due, if only in part, to enhanced IGFBP-3 mediated antigonadotropic activity at the ovarian level. -
Role and regulation of Jumonji C domain-containing histone demethylases 1A and 2B in pulmonary hypertension

Pulmonary hypertension (PH) is characterized by increased proliferation and apoptosis resistance of pulmonary vascular cells. Jumonji C domain-containing histone demethylases (JMJDs) are a novel class of epigenetic regulators, implicated in chromatin regulation and often possess the ability to demethylate lysine residues on histones. Given that the JMJDs and the histone methylation level is important for the proliferative capacity of cancer cells, we postulated the contribution of JMJDs to excessive vascular remodeling underlying PH. Expression of JMJDs was analyzed in vascular cells exposed to hypoxia. Increased expression of JMJD1A and JMJD2B were observed in all three cell types. Increased nuclear localization and activity of JMJD1A and JMJD2B were observed in hypoxic PASMCs. Increased expression of JMJD1A and JMJD2B was dependent on hypoxia induced factor 1α (HIF1α). Importantly, selective knockdown of these JMJDs by siRNA and JMJD inhibitors led to inhibition of proliferation and induction of apoptosis in vascular cells exposed to hypoxia. Furthermore, in laser micro-dissected vessels and pulmonary arteries from idiopathic pulmonary arterial hypertension (IPAH) patients, increased expression of JMJD1A and JMJD2B was observed compared to donors. Next generation RNA-sequencing of hypoxia treated PASMCs with JMJD1A and JMJD2B knockdown lead to identification of promising JMJD target genes, which are involved in biological processes like proliferation (MKI67), apoptosis (BCL2, BIRC5), metabolism (LDHA, TK1) and inflammation (CCR7, CCL3). In conclusion, our results suggest that JMJDs are regulated in PH vasculature and play an important role in PH pathogenesis.
Prediction of body weights from body measurements in East Anatolian Red calves

Relationships between liveweight and body measurements were examined for East Anatolian Red calves. Live weights and body measurements were recorded at birth, weaning and 6 months of age. The analyses revealed strong relationships between weight and the body measurements at each age and across all ages. Prediction at each age was most accurate using a combination of measurements, but chest girth alone accounted for a large amount of variation. It is concluded that chest girth can be used as a predictor of body weight in East Anatolian Red cattle calves.
Species diversity of pollen beetles (Meligethess.l.: Coleoptera, Nitidulidae) in oilseed rape and resistance of(Meligethes aeneus (F.) to pyrethroids

Research aim and objectives. To investigate species diversity of pollen beetles (Meligethes s.l.) in WOSR and SOSR crops during flowering stage and to evaluate the resistance of pollen beetle (M. aeneus) to pyrethroid class insecticides. Research objectives: ● To estimate species diversity of pollen beetles (Meligethes s.l.) in WOSR and SOSR agrocenoses during flowering stage in the regions of intensive oilseed rape growing by morphologic and molecular (DNA sequencing) methods and to evaluate the species diversity (Simpson’s) index; • To evaluate the genetic diversity of the most common species of pollen beetles (Meligethes s.l.) in oilseed rape agrocenoses and to compare them with the deponated data in the GenBank; • To evaluate the resistance of populations of pollen beetle (M. aeneus), collected from different regions, to active substances of pyrethroid class insecticides: λ- cyhalothrin, α- cypermethrin, deltamethrin and tau – fluvalinate by IRAC (Insecticide Resistance Action Committee) method and to determine the variation in the resistance of pollen beetle during the research period; • To determine the resistance of pollen beetle (M. aeneus) to pyrethroid class insecticides in the field conditions; • To determine the effectiveness of insecticides with different modes of action (pyrethroid, neonicotinoid, phosphorus-organic) against pollen beetle (M. aeneus) in the field conditions and to evaluate their effect on the productivity of WOSR and SOSR. Scientific novelty of. [to full text]%%%%Tyrimų tikslas ir uždaviniai. Istirti žiedinukų (Meligethes s.l.) rūsine sudėtį žieminių ir vasarinių rapsų pasėliuose žydėjimo tarpsniu, atskirų rūsių isplitimą bei įvertinti rapsinio žiedinuko (M. aeneus) atsparumą piretroidų klasės insekticidams. Tyrimų uždaviniai: • Nustatyti žiedinukų (Meligethes s.l.) rūsine sudėtį žieminių ir vasarinių rapsų agrocenozėse žydėjimo tarpsniu intensyvaus rapsų auginimo regionuose morfologiniu ir molekuliniu (DNR sekoskaitos) metodais bei įvertinti žiedinukų (Meligethes s.l.) rūsių isplitimo indeksą; • Įvertinti dažniausiai aptinkamų rapsų agrocenozėse žiedinukų (Meligethes s.l.) rūsių genetinį polimorfizmą bei palyginti su Genų banke deponuotais duomenimis; • Įvertinti rapsinio žiedinuko (M. aeneus) populiacijų is skirtingų regionų atsparumą piretroidų klasės insekticidų veikliosioms medžiagoms: lambda-cihalotrinui, alfa-cipermetrinui, deltametrinui ir tau-fluvalinatui IRAC metodu bei nustatyti rapsinio žiedinuko atsparumo pokycius per tyrimų laikotarpį; • Nustatyti rapsinio žiedinuko (M. aeneus) atsparumą piretroidų klasės insekticidams lauko sąlygomis; • Nustatyti skirtingų klasių insekticidų (piretroidų, neonikotinoidų, fosforoorganinių junginių) veiksmingumą nuo rapsinio žiedinuko (M. aeneus) lauko sąlygomis bei įvertinti jų poveikį žieminių ir vasarinių rapsų derlingumo rodikliams. Mokslinis darbo naujumas. Morfologiniu bei DNR sekvenavimo metodais nustatyta Meligethes s.l. populiacijų rūsinė sudėtis bei įvertintas rūsių isplitimas. [toliau žr. visą tekstą]
Capital social et gestion des conflits dans le périmètre irrigué de Malanville au Bénin

Cet article analyse l’influence du capital social dans la gestion des conflits au sein des systemes d’irrigation autogeres a travers le cas du perimetre irrigue de Malanville au Benin. Il montre que le capital social d’attachement est efficace lorsque le conflit est lie au non-respect des regles operationnelles. Le capital social de contact contribue a la destruction du capital social d’attachement dans un conflit lie au non-respect des regles collectives et constitutionnelles. La mediation des services de l’Etat est alors necessaire pour resoudre le conflit. Ces resultats suggerent une necessaire synergie entre les structures autogerees et l’Etat, pour la resolution effective des conflits.
PRELIMINARY PHYTOCHEMICAL ANALYSIS OF BLACK GRAM, Vigna mungo L.

Black gram ( Vigna mungo L.) is an important pulse crop occupying unique position in Indian agriculture. The study of phytochemicals, secondary metabolic substances in plants are known to provide protection against insect attacks and plant diseases. The current investigation is a n attempt to study the preliminary phytochemicals that are present in black gram using different solvent extracts. An experiment was conducted to study the influence of chemical fertilizers (DAP and superphosphate) and leaf extracts ( Ocimum basilicum and L antana camara L.) on the presence of secondary metabolites. The results showed the presence of flavonoids, terpenoids, quinone, sterols, oil and fat by various tests.
Analyses of yield and economic response from foliar fungicide and insecticide applications to soybean in the North Central United States

Foliar disease and insect management on soybean (Glycine max L. Merrill) in the North Central region of the United States has been increasingly accomplished through foliar fungicide and insecticide application. Data from research trials conducted in Illinois, Indiana, Iowa, and Nebraska were compiled from 2008 to 2014 to determine the impact of fungicide, insecticide, and fungicide + insecticide applications on soybean yield and profitability. In each state, field experiments occurred each year in two to seven locations. All treatments were applied at the R3 growth stage. Disease and insect pressure were very low in all states and years. A foliar application of fungicide, insecticide, or the combination, increased yield in seven out of 14 total site-years (P < 0.10). Economic analysis using an average soybean price of $0.42 per kilogram and average application cost of $62 per hectare indicated that fungicide applications were only profitable in 14% of the trial site-years. Insecticide alone and fungicide + insecticide was profitable in 39% and 45% of site-years, respectively. Effect of fungicide class on yield was inconsistent. Our results indicate that although yield increases can occur with foliar fungicide and/or insecticide treatments, current market prices and application costs may limit profitability when disease and/or insect pressure is low.
Expression and significance of HIF-1α and Glut-1 in basal-like breast carcinoma

Objective: To investigate the expression and clinical significance of hypoxia inducible factors 1α (HIF-1α) and glucose transporter 1 (Glut-1) in basal-like breast carcinoma (BLBC). Methods: The protein expressions of HIF-1α and Glut-1 in 95 specimens of BLBC and their adjacent breast tissue, and 708 samples of non-BLBC tissue were detected by immunohistochemical staining, and their relations with the clinicopathologic factors of BLBC were analyzed. Results: The positive expression rate of either HIF-1α or Glut-1 protein in BLBC tissue was significantly higher than that in adjacent breast tissue and non-BLBC tissue (all P<0.05), and both HIF-1α and Glut- 1 protein expressions were associated with lymph node metastasis and clinical stage of BLBC (all P<0.05); there was a significantly positive correlation between HIF-1α and Glut-1 expression in BLBC tissue (r=0.368, P<0.05). ·基础研究·
Lipid profile in blood and in plaque material from diseased coronary artery.

Moreover, we have analysed the fatty acid composition of the plaques taken from diseased coronary arteries has been analysed (supplying heart muscle). A total of 71 samples of plaques were analysed, of which 48 persons were using coconut oil and 23 persons were using sunflower oil routinely. Fatty acids were extracted by chloroform and then analysed by HPLC (high performance liquid chromatography). It was seen that Plaques from coronary artery did not contain fatty acids from coconut oil. Fatty acid content of plaques from coconut oil group and sunflower group were the same.This clearly Lipid Profile in blood and in plaque material from diseased Coronary Artery
Efectos del piojo del salmón Lepeophtheirus salmonis (Copepoda: Caligidae) en las poblaciones de truchas (Salmo trutta) de la costa NE Atlántica

Los piojos del salmon son parasitos externos de salmonidos en ambientes marinos. En los ultimos anos, la abundancia de este parasito ha aumentado debido a la presencia de cultivos de salmones en jaulas de engorde en mar abierto. Entre los salmonidos, la trucha es especialmente vulnerable a las infecciones por el piojo ya que permanece en aguas costeras durante su periodo marino, donde se situan las instalaciones acuicolas de cultivo del salmon. En el presente trabajo se recopila la informacion existente sobre los efectos del piojo del salmon en las poblaciones de truchas silvestres que habitan las costas del NE Atlantico, y evaluar posteriormente el estado actual de dicha problematica. La acuicultura de salmon aumenta la abundancia de piojos, los cuales impactan negativamente sobre las poblaciones de truchas, aumentando la mortalidad, cambiando las pautas migratorias y disminuyendo el crecimiento. Estas conclusiones se basan en estudios cientificos que abarcan desde experimentos en el laboratorio o en el campo de los efectos del piojo en las truchas a nivel individual, hasta estudios poblacionales de dichos impactos. En resumen, el conocimiento adquirido en esta revision evidencia la existencia de un efecto general y persuasivo de los piojos en las poblaciones de truchas, especialmente en areas de cultivo intensivo. Los efectos derivados de altos niveles de infestacion implican inevitablemente una reduccion en la abundancia y el tamano corporal de las truchas que vuelven a agua dulce a reproducirse, afectando a la dinamica poblacional a nivel local, asi como a pescadores deportivos y comerciales.
Intertidal plant communities of an Arctic salt marsh: The influence of isostatic uplift and herbivory

Abstract Recently, the mid-continent population of lesser snow geese has increased at about 7% per year and increased numbers of geese have adversely affected the vegetation of intertidal communities on the shores of Hudson and James Bays. A quantitative description of plant communities was undertaken before they are modified or lost as a result of the direct and indirect effects of goose foraging. The region is undergoing isostatic uplift and salt marshes have developed on the extended, low elevation shorelines. The site chosen for the study was La Pérouse Bay, Manitoba, where there is a breeding colony of lesser snow geese. The plant assemblages recognised in intertidal zone, based on the use of Canonical Correspondence Analysis, were the Puccinellia-Carex community, the Festuca-Calamagrostis community (both of which were grazed and grubbed by geese), bare sediment, and the Hippuris-Myriophyllum assemblage characteristic of brackish aquatic habitats (rarely grazed by geese). The communities were primarily differentiated in terms of elevation, water depth, and area of plant cover. These communities are widespread elsewhere in intertidal areas adjacent to the Hudson Bay lowlands, but intensive goose foraging has led either to their loss or to fine-scale fragmentation with little likelihood of revegetation in the foreseeable future. This description of vegetation serves as a baseline against which further changes can be monitored and assessed.
Nucleotide Sequence Analysis of an Endo-Xylanase Gene (xynA) from Bacillus stearothermophilus

A gene (xynA) encoding the endo-xylanase (E.C.3.2.1.8) from Bacillus stearothermophilus was cloned in E. coli, and its complete nucleotide sequence was determined. The xynA gene consists of a 636 base pairs open reading frame coding for a protein of 212 amino acids with a deduced molecular weight of 23, 283 Da. A putative signal sequence of 27 amino acid residues shows the features comparable with the Bacillus signal sequences; namely, the signal contains a positively charged region close to the N-terminus followed by a long hydrophobic string. The coding sequence is preceded by a possible ribosome binding site with a free energy value of -16.6 kcal/mol and the transcription initiation signals are located further upstream. The translation termination codon (TAA) at the 3 end of the coding sequence is followed by two palindrome sequences, one of which is thought to act as a terminator. The xynA gene has a high GC content, especially in the wobble position of codons (64%). Comparison of the primary protein sequence with those of other xylanases shows a high homology to the xylanases belonging to family G.
Effect of Dietary Sodium Tetraborate on Adult Longevity and Fecundity of Drosophila melanogaster (Diptera: Drosphilidae)

Abstract The fruit fly Drosophila melanogaster (Meigen) (Diptera: Drosophilidae) is often used in various biological, molecular, and toxicological studies. Sodium tetraborate, a boron compound, was added to the artificial diet of developing D. melanogaster to determine its effects on adult longevity and fecundity. Insects reared from first instars to adults on diets containing 10, 30, 150, 300, or 400 mg/liter sodium tetraborate showed that the highest concentration (400 mg/liter) significantly reduced female longevity (31.65 ± 4.02 [mean ± SD] d for the controls versus 1.87 ± 0.30 d for 400 mg/liter) and male longevity (32.80 ± 1.96 d for controls versus 3.57 ± 0.42 d for 400 mg/liter). Females from the control diet produced 9.46 ± 0.57 (mean ± SD) eggs per female, whereas those fed on a diet containing 300 mg/liter produced only 1.92 ± 0.30 eggs per females. These results expand our knowledge of the impact of sodium tetraborate on various insects and indicate that boron compounds should be further investigated to ascertain their potential as an alternative control tactic for pest insects.
Dynamics of grape berry growth and physiology of ripening.

Data from two experiments on development of grape berries is re-examined with emphasis on partitioning of berry weight into non-solutes per berry (largely water) and solutes per berry (largely sugar), using weight times juice °Brix. This approach is based on the thought that, since xylem flow is blocked after veraison, time curves of solutes per berry indicate the activity of phloem transport into the berry during ripening growth. Experiment 1: Measurements of Muscat Gordo Blanco berries from inflorescences with a spread of flowering times showed typical double-sigmoid volume/time curves but with divergent rates and amounts of volume increase. Despite this divergence, °Brix curves after veraison were almost coincident because, in each case, the rate of increase in solutes per berry was proportional to that of berry volume. These results indicate that sugar and water increments after veraison are linked and depend on the same source, namely, phloem sap. Experiment 2: An irrigation experiment on cv. Shiraz also showed divergent berry weight curves between treatments and years but with the difference that all berries shrank after a maximum berry weight was attained at 91 days after flowering (at about 20 °Brix). At this point, the curves of solutes per berry slowed then plateaued, indicating that inflow of phloem sap had become impeded. Prior to shrinkage these berries accumulated primary metabolites (mainly phloem sugar) but, during shrinkage, when berries were apparently isolated from vascular transport, non-anthocyanin glycosides accumulated. These results have implications for the study of berry flavour build-up and berry composition, and also for the understanding of sink competition within the vine, fresh and dried yield, and juice °Brix levels.
Germination of Developing Prickly Sida Seeds

Freshly-produced, mature prickly sida (Sida spinosa L.) seeds (18 to 21 days after anthesis, < 20% water content) were dormant and neither imbibed water nor germinated when incubated for up to 4 weeks under several light and temperature conditions. Over 80% of the freshly-produced, immature seeds (12 to 16 days after anthesis, > 20% water content) germinated when removed from the mother-plant before dehydration and incubated in alternating 20 to 30 C for 4 weeks. The onset of dormancy coincided with the later stages of seed dehydration and coat-hardening. Over 90% of the mature seeds imbibed water and germinated when incubated at 35 C after 4 months dry storage at 25 C. A puncture through the seed coat, either over the radicle or cotyledons, permitted water imbibition by all mature seeds, but the puncture over the radicle was significantly more effective in inducing germination. Seed coat impermeability was important, but was not the only factor responsible for prickly sida dormancy.
Genetic characterization of Southern African sheep breeds using DNA markers

5 Preface.7 CHAPTER 1 – General Introduction . 9 CHAPTER 2 – Material and Methods. 20 CHAPTER 3 – Results and Discussion. 31 CHAPTER 4 – Conclusions . 60 CHAPTER 5 – References. 63 CHAPTER 6 – Appendices . 76 APPENDIX 1 . 77 APPENDIX 2 . 97 APPENDIX 3 . 131
A Novel Effect for Glycine on Root System Growth of Habanero Pepper

Amino acids, a major fraction of the low-molecular-weight organic nitrogen in soil, act as signaling molecules that indicate the presence of nutrient-rich patches to the roots. To characterize the effects of amino acids on root growth, we used seedlings of habanero pepper (Capsicum chinense), one of the most widely cultivated annual spice crops in the world. We tested the effect of L-glutamate, L-aspartate, and glycine on the primary root of seedlings grown aseptically under different conditions of pH and light. L-glutamate and L-aspartate did not inhibit the root growth of habanero pepper. In contrast, glycine inhibited the growth of roots, stimulated root hair growth, and induced a significant accumulation of starch grains in the root apex. The use of aminoethoxyvinylglycine, an inhibitor of ethylene biosynthesis, and the evaluation of 1-aminocyclopropane-1-carboxylic acid oxidase expression provided evidence of a role for ethylene in the root responses to glycine. We suggest that these changes in the root apex in response to exogenous glycine could be an important adaptive response that allows plants to efficiently access the fluctuating availability of nutrients in the soil.