Miracle-dz/UniFAR-FTUs huggingface dataset:
The Drosophila SWI/SNF chromatin-remodeling complexes play separate roles in regulating growth and cell fate during regeneration

Regeneration is a precise process that requires regulated cell proliferation and accurate cell fate decisions. The mechanisms that temporally and spatially control the activation or repression of important genes during regeneration are not fully understood. Epigenetic modification of regeneration genes by Polycomb group and Trithorax group chromatin regulators could be key factors that govern gene repression and activation in damaged tissue. Here we report a genetic screen of chromatin regulators that identified phenotypes for many PcG and TrxG mutants and RNAi lines in Drosophila imaginal wing disc regeneration. Furthermore, we show that the two Drosophila SWI/SNF chromatin-remodeling complexes, BAP and PBAP, play distinct roles in regeneration. The PBAP complex regulates regenerative growth and developmental timing, and is required for the expression of JNK signaling targets and upregulation of the growth promoter myc. By contrast, the BAP complex is required for ensuring proper patterning and cell fate by stabilizing expression of the posterior gene engrailed. The core complex components are required for both processes: a weak knockdown of brahma (brm), which encodes the only ATPase of the SWI/SNF complexes, induces the BAP mutant regeneration phenotype, whereas a strong brm mutant shows the PBAP regeneration pheno-type. Thus, both SWI/SNF complexes are essential for proper gene expression during tissue regeneration, but play distinct roles in regulating growth and cell fate.
Disease Progression in Monoclonal B-Cell Lymphocytosis Is Independent of VH Mutation Status.

A monoclonal B-cell Lymphocytosis (MBL) is detected in the peripheral blood of around 3% of otherwise healthy adults, the majority of these have a CLL immunophenotype. We have previously demonstrated that the cells in MBL are indistinguishable from good risk CLL, sharing the same immunophenotypic profile, genetic aberrations and IgVH gene usage. MBL exerts an increasing burden on haematology clinics with over 100 new patients diagnosed per annum in our regional haemato-oncology laboratory. This is largely as a result of the increased tendency to investigate patients with a mild lymphocyosis although 10,000/μL and included 3 patients who progressed to a clinical stage requiring treatment. VH gene usage was different in each of these 3 patients was with VH3-7, VH4-34 and VH5-51 identified, the levels of SHM were 5.8, 4.0 and 5.3% respectively. Follow-up data shows that a deletion of ATM was detected at progression in one of these patients. In conclusion, we show that MBL is predominantly mutated and that progression to CLL is independent of mutational status. Other prognostic markers, particularly assessment of chromosomal aberrations, may be informative but this would probably require cell selection to increase sample purity. Our data suggests that current prognostic markers are not effective at predicting outcome in MBL and periodic monitoring is required.
AVALIAÇÃO DOS EFEITOS DE Beauveria bassiana (HYPOCREALES: CORDYCIPITACEAE) E ÓLEOS ESSENCIAIS DE Pogostemon cablin (LAMIALES: LAMIACEAE) SOBRE O DESENVOLVIMENTO EMBRIONÁRIO INICIAL DE Gallus gallus (GALLIFORMES: PHASIANIDAE)

Testes com ovos embrionados de galinha, usando o fungo entomopatogenico Beauveria bassiana e oleo essencial de Pogostemon cablin (Patchouli), foram realizados para avaliacao de seus efeitos sobre o desenvolvimento inicial de vertebrados. Os ovos receberam injecao na câmara de ar das diferentes solucoes testadas, previamente a incubacao, exceto os do grupo controle fechado, os quais nao foram injetados. Em seguida, os ovos foram incubados por tres dias e abertos para a contabilizacao de embrioes que desenvolveram e estavam vivos, embrioes que continuaram a se desenvolver e morreram e embrioes inviaveis, que nem retomaram o desenvolvimento. Os embrioes vivos foram entao eutanasiados, coletados, preparados por montagem total e analisados morfologicamente para a determinacao do estagio e verificacao da presenca de malformacoes. De acordo com os dados obtidos, verificou-se que o tratamento com a suspensao do fungo entomopatogenico B . bassiana induziu maiores taxas de inviabilidade embrionaria, mortalidade e malformacoes, enquanto que as menores foram encontradas no tratamento com o oleo essencial. Embora essas taxas tenham sido menores no tratamento com o oleo essencial, elas foram semelhantes no tratamento utilizando somente o veiculo (Tween 80). Desta forma, concluiu-se que testes futuros devem ser realizados, utilizando outro(s) veiculo(s).
iPhoma macrostoma/i: as a broad spectrum bioherbicide for turf grass and agricultural applications.

Phoma macrostoma Montagne 94–44B is an effective bioherbicide for broadleaved weed reduction. In 2016, Health Canada’s Pest Management Regulatory Agency (PMRA), under the authority of the Pest Control Products Act and Regulations, granted full registration for the sale and use of the bioherbicide P. macrostoma Montagne 94–44B to control a broad spectrum of broadleaf weeds in established turfgrass and new seeding of grasses as well as in field grown nursery plants, trees and container-grown ornamentals. P. macrostoma 94–44B colonizes susceptible and non-susceptible plant roots, however, only in susceptible plants, such as Taraxacum officinale (dandelion), do mycelium proliferate around the vascular trachea interfering with the function of neighbouring cells while not entering them. Macrocidins, secondary metabolites secreted by P. macrostoma 94–44B mycelia, inhibit multiple steps in carotenoid precursor formation including phytoene desaturase and steps associated with β carotene and lutein carotenoid biogenesis, Fe and Mg chelation and OJIP chlorophyll fluorescence, thus uncoupling the light-harvesting complex of photosystem II from the reaction centre. The combined actions of P. macrostoma 94–44B and macrocidins elicit photobleaching of the leaves and eventual weed death. Research is underway to exploit Phoma’s bioherbicidal actions for weed management in agriculture. P. macrostoma 94–44B can be used to reduce broadleaved weeds in crops such as wheat, corn, potato, forage grasses and established alfalfa (2nd growth or older). Soil residue studies reveal that neither P. macrostoma 94–44B nor macrocidins are present after 1 year suggesting that farmers could plant Phoma-sensitive crops such as legumes, flax or canola in subsequent years.
In vivo amelioration of aflatoxin B1 in broiler chicks by magnetic carbon nanocomposite

Zafar R. , Khan F.A. & Zahoor M. 2017. In vivo amelioration of aflatoxin B1 in broiler chicks by magnetic carbon nanocomposite. Pesquisa Veterinária Brasileira 37(11):1213-1219. Department of Chemistry, University of Malakand, Chakdara, Dir Lower, 18800 KPK, Pakistan. E-mail: mohammadzahoorus@yahoo.com In this study an Iron oxide/carbon nanocomposite from maize straw was prepared and was characterized by XRD, SEM, EDX, FTIR, TG/DTA and Surface area analyzer. The adsorbent was fed to different groups of poultry birds along with aflatoxin B1. Different physiological and blood parameters were monitored in order to study the efficacy of the prepared adsorbent for binding of aflatoxin B1 in the gastrointestinal tract of chickens. It was found that adsorbent at dose of 0.3%/ kg feed was highly effective in detoxifying aflatoxin B1 in gastrointestinal tract of poultry birdswith no harmful effects. The high doses given to groups E and F; 0.4% and 0.5% respectively showed slight variation in tested parameters from group A. No negative symptoms associated with the use of activated carbon as previously reported were observed for the adsorbent under study.
FogWise: On the limits of the coexistence of heterogeneous applications on Fog computing and Internet of Vehicles

Mobile devices, Internet of Things applications, and connected vehicles in the Internet of Vehicles (IoV) are proliferating. Recently, vehicles have emerged as a possibility to become communication and processing infrastructures called vehicular Fog computing (VFC). Thus, science and industry forecast increasing demand for communication and cloud infrastructures to deal with data traffic, processing, and storage. Still, they do not evaluate that properly. This work investigates how heterogeneity affects the ability of IoV and VFC to meet application requirements. We leverage Fog computing, which extends Edge and Cloud computing and complements them, especially to meet str applications with strict latency requirements. We built a simulation model that evaluates how vehicles behave in such an environment. We argue that vehicles need to follow request‐processing‐response‐actuation programming model based on distributed auction protocol to match clients and servers and still meet latency requirements. We extend and propose requirements that both applications and architectures should meet. Preliminary results indicate thresholds in terms of vehicle density, request rate, request size, client‐server ratio, and auction method in which VFC can operate to meet application requirements under mobility.
Effects of Chopping Length and Additive on the Fermentation Quality and Aerobic Stability in Silage of Leymus chinensis

The objective of this experiment was to evaluate the effects of the chopping length and additive on the fermentation characteristics and aerobic stability in silage of Leymus chinensis. L. chinensis was chopped to 1–2 cm and 4–5 cm, and immediately ensiled with the three treatments, i.e., 2% sucrose (fresh weight basis; SU), 1 × 105 cfu/g Lactobacillus plantarum (LP) or 1 × 105 cfu/g LP plus 2% sucrose (SU+LP). Silage treated with distilled water served as the control. After silage processing for 30 and 90 d, the fermentation quality of L. chinensis silage was evaluated. The composition of the fermentation products and the pH value in the silage were determined at 1, 3, 5 and 7 d after opening the silo. The results showed that in L. chinensis silage there was a lower pH value, higher lactic acid content and better aerobic stability at the 1–2 cm length than those at the 4–5 cm (p < 0.001). When the chopping length was 4–5 cm, the addition of either LP or SU+LP increased the content of lactic acid and acetic acid, and decreased the pH value and butyric acid content, compared to those of the control and SU treatment (p < 0.001). Furthermore, combination treatment of SU+LP performed better than LP alone, and the aerobic stability time of L. chinensis silage at 4–5 cm without any additives was the worst. In conclusion, enhanced fermentation quality and aerobic stability can be obtained by processing L. chinensis silage with the shorter length. When the L. chinensis is cut longer, e.g., 4–5 cm in this study, LP or SU+LP could be used as an effective method to improve the fermentation quality and aerobic stability of L. chinensis silage.
Male sexual competitiveness of two Ceratitis capitata strains, tsl Vienna 8 and OX3864A transgenics, in field cage conditions

The success of the sterile insect technique (SIT) depends on the ability of sterile males to compete effectively with wild males to mate with wild females. This study compared the sexual competitiveness and other behavioral parameters of males of two strains of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), tsl Vienna 8 (tsl V‐8) and the transgenic line OX3864A, in competition with wild males from the São Francisco Valley region in Bahia, Brazil. Mating events were observed in field cages containing virgin, sexually mature insects as follows: 50 wild females (10–12 days old), 50 wild males (8–10 days old), and either 50 tsl V‐8 sterile males (5 days old) or 50 transgenic males (3–4 days old). In addition to scoring male type for each mating event, we also monitored the location of mating events within the tree canopy, mating duration, and mating latency. The proportion of females that mated did not differ between the two competition scenarios tested (tsl V‐8 vs. wild males: 0.65 ± 0.04; transgenic vs. wild males: 0.64 ± 0.03). Relative sterility index did not differ between sterile (0.34 ± 0.04) and transgenic males (0.31 ± 0.03). The latency period was significantly longer for transgenic males (84.90 ± 4.87 min) than for sterile (65.24 ± 4.65 min) and wild males (66.61 ± 2.66 min). Mating events involving the three strains occurred in the same area of the tree, i.e., mid‐height, in the central sector and on leaf undersurfaces. The mean duration of mating events involving sterile (94.1 ± 3.59 min) and transgenic males (81.1 ± 3.54 min) were both significantly shorter than for wild males (123.3 ± 1.93 min). Further investigation is suggested to assess other behavioral parameters, such as the amount of sperm transferred by transgenic males, their ability to disperse and survive in the field conditions, and their capacity to prevent females remating with wild males, in order to determine the potential of this transgenic strain for inclusion in the Brazilian SIT programs.
Screening of Lactic Acid Bacteria Suitable for Silage-Making in Tropical Regions

Lactic acid bacteria suitable for silage-making under tropical conditions were screened based on experiment on liquid culture and model system for silage fermentation (pouch method). We selected a lactic acid bacterium strain I-7 from many isolates from silage. The strain belonging to the genus Lactobacillus showed a good adaptability to silage making at 45-52°C. Furthermore, the latic acid productivity (LAP) of the strain was inhibited by the addition of lactic acid to the liquid medium. However, the degree of inhibition was lower than that of other strains and remained a level of approximately 80 and 40% of that of the initial LAP in the presence of 0.5 and 1.0% lactic acid, respectively. Discipline: Animal industry Additional key words: thermotolerant strain, tropical silage
Diet and feeding observations from an unusual beluga harvest in 2014 in Ulukhaktok, Northwest Territories, Canada

The Eastern Beaufort Sea (EBS) beluga (Delphinapterus leucas) population are an important traditional food for the Inuit of the Inuvialuit Settlement Region, Northwest Territories (NT) Canada. In 2014, over 30 beluga whales were harvested at Ulukhaktok, NT, the first occurrence of a large harvest in the area on record. Unlike observations from the established beluga harvest monitoring program in the Mackenzie Estuary, these belugas had numerous prey and prey items in their stomachs. Our study objectives were to combine traditional and local knowledge (TLK) from beluga hunters with the analysis of dissected stomachs to identify EBS beluga diet, feeding behaviour, as well as gain insights into potential drivers of the event. TLK holders witnessed foraging behaviors such as herding schools of fish. Stomach dissections revealed Sandlance (Ammodytes sp.) to be the predominant prey species, comprising 90% of identified otoliths, occurring in 92% of stomachs. The low presence of Arctic Cod (Boreogadus saida), a .
Investigation of Ring Finger Protein 8 in Mammalian Physiology and Pathogenesis

While genomic stability is often viewed as a cellular shield against malignant transformation, DNA double-strand breaks (DSBs) are probably the most dangerous threat to the maintenance of a stable cellular genome. Mammalian cells have evolved a sophisticated DNA damage response (DDR) network that senses the physical existence of DNA DSBs, followed by signal propagation to repair these genetic lesions. Interestingly, DSBs are not only hazardous, but are also programmed to occur during normal physiological processes such as immunoglobulin heavy chain (IgH) class switch recombination (CSR), an important mechanism for antibody diversification and specification of immunoglobulin effector function during a humoral immune response in mammals. Through catalyzing ubiquitylation of the H2A-type histones flanking DNA DSBs, the E3 ligase Ring finger protein 8 (Rnf8) orchestrates the assembly of components of homologous recombination (HR) and nonhomologous end joining (NHEJ) repair machineries into DSBinduced foci. To examine the in vivo functions of Rnf8 during mammalian development and determine the effects of its deficiency on the development of various diseases, we generated Rnf8 knockout mouse models and elucidated the pleiotropic in vivo functions of Rnf8. Overall, this thesis highlights the physiological significance of Rnf8 in shielding against DNA DSB repair defects, genomic instability, male infertility, immunodeficiency and cancer development at the organism level.
Current Advances in Plant Growth Promoting Bacteria Alleviating Salt Stress for Sustainable Agriculture

Humanity in the modern world is confronted with diverse problems at several levels. The environmental concern is probably the most important as it threatens different ecosystems, food, and farming as well as humans, animals, and plants. More specifically, salinization of agricultural soils is a global concern because of on one side, the permanent increase of the areas affected, and on the other side, the disastrous damage caused to various plants affecting hugely crop productivity and yields. Currently, great attention is directed towards the use of Plant Growth Promoting Bacteria (PGPB). This alternative method, which is healthy, safe, and ecological, seems to be very promising in terms of simultaneous salinity alleviation and improving crop productivity. This review attempts to deal with different aspects of the current advances concerning the use of PGPBs for saline stress alleviation. The objective is to explain, discuss, and present the current progress in this area of research. We firstly discuss the implication of PGPB on soil desalinization. We present the impacts of salinity on crops. We look for the different salinity origin and its impacts on plants. We discuss the impacts of salinity on soil. Then, we review various recent progress of hemophilic PGPB for sustainable agriculture. We categorize the mechanisms of PGPB toward salinity tolerance. We discuss the use of PGPB inoculants under salinity that can reduce chemical fertilization. Finally, we present some possible directions for future investigation. It seems that PGPBs use for saline stress alleviation gain more importance, investigations, and applications. Regarding the complexity of the mechanisms implicated in this domain, various aspects remain to be elucidated.
Comparative study of innovative blends prepared by fortification of date powder to alleviate child malnutrition

Abstract Child malnutrition is one of the biggest problems in developing countries with higher level of food insecurity. Pakistan is 5th largest producer of date fruit; therefore, its processing and products should be explored in various dimensions. Being rich source of minerals and sugars, it can contribute in weaning foods in a good manner. In current study, three blends were prepared with specific proportions of spray‐dried date powder and rich in specific proportions. They were compared with each other and control (free of date powder). The nutritional profile of the formulations revealed that 100 g of each formulation included all macronutrients in compliance with the requirements of Food and Agriculture Organization for weaning formulations; moreover, they are enriched with minerals due to presence of date powder. The iron contents reached up to 12.74 ± 0.16 mg/100 g. The phosphorus, zinc, and potassium contents also increased with the increase in date powder subsequently. Physicochemical properties exhibited in compliance with the requirement of the weaning foods. The protein quality was assessed both in vivo and in vitro. Amino acid profiling indicated that the limiting amino acid in F1 and control were lysine but in F2 and F3 were threonine. This is suggested that date powder might contain good quality protein that was further explained in biological studies, the formulations that contained higher amount of date powder reveled better PDCAAS score 86.76 ± 4.5, true digestibility 84 ± 4.36, biological value 69.45 ± 0.69, net protein utilization 73.82 ± 1.46, and protein efficiency score 1.18 ± 0.07. The sensory evaluation revealed that F2 showed better result in overall acceptability. Thus, date powder is suggested to be used as good constituent that can fortify mineral contents and sugar contents of the weaning foods.
SEED PRIMING: A POTENTIAL STRATAGEM FOR AMELIORATING SOIL WATER DEFICIT IN WHEAT

Wheat is an important cereal crop serving as staple food for more than 1/3 of the world’s population. But global climate change events have increased the frequency of drought spells leading to less harvest of the crop (Richards et al., 2001). The soil water deficit conditions resulted in reduced chlorophyll contents, leaf area, number and weight of grains with poor grain set and development in wheat (Farooq et al., 2014). The extent of yield loss is directly related to the duration of water stress as well as crop growth stages. The soil water deficit at crown root initiation to milking growth stage caused 30-37% yield reduction in wheat as compared to normal irrigation at the said growth stages (Kahlown et al., 2003). Similarly, yield reduction of wheat in relation to mild to severe soil water deficit stress has been recorded up to 37% at booting to maturity (Shamsi et al., 2010), , 57% at heading (Balla et al., 2011), 18–53% at pre-anthesis (Majid et al., 2007), 11–39% at anthesis (Jatoi et al., 2011), 1–30 % at post-anthesis (Eskandari and Kazemi, 2010) and 9–78% at grain filling (Guoth et al., 2009) stages. However, it is a dire need to identify comparatively more critical pre and post anthesis growth stages of wheat for irrigation requirements under field conditions. The main aim of current study was to minimize yield reduction under limited supply of water with improved management practices.
Impact of Lymph Node Yield in Patients Undergoing Total Laryngectomy and Neck Dissection

Objectives: To determine the impact of lymph node yield (LNY) in patients undergoing neck dissection at the time of total laryngectomy (TL). To determine the impact of radiation therapy (RT) on LNY. Methods: Retrospective review of LNY and clinical outcomes in 232 patients undergoing primary or salvage total laryngectomy (TL) with ND. Results: Preoperative RT significantly decreased mean LNY from 31.7 to 23.9 nodes (P < .001). In primary TL patients, age (P < .001) and positive margins (P = .044) were associated with decreased OS. In salvage TL patients, only positive margins was associated with poorer OS (P = .009). No LNY cutoff provided significant OS or DFS benefit. Conclusions: Radiotherapy significantly reduces LNY in patients undergoing TL and ND. Within a single institution cohort, positive margins, but not LNY, is associated with survival in both primary and salvage TL patients. Level of Evidence: 4