Miracle-dz/UniFAR-FTUs huggingface dataset:
Serine 19 of Human 6-Pyruvoyltetrahydropterin Synthase Is Phosphorylated by cGMP Protein Kinase II*

6-Pyruvoyltetrahydropterin synthase (PTPS) participates in tetrahydrobiopterin cofactor biosynthesis. We previously identified in a PTPS-deficient patient an inactive PTPS allele with an Arg16 to Cys codon mutation. Arg16 is located in the protein surface exposed phosphorylation motif Arg16-Arg-Ile-Ser, with Ser19 as the putative phosphorylation site for serine-threonine protein kinases. Purification of recombinant PTPS-S19A from bacterial cells resulted in an active enzyme (k cat /K m = 6.4 × 103 m −1 s−1), which was similar to wild-type PTPS (k cat /K m = 4.1 × 103 m −1 s−1). In assays with purified enzymes, wild-type but not PTPS-S19A was a specific substrate for the cGMP-dependent protein kinase (cGK) type I and II. Upon expression in COS-1 cells, PTPS-S19A was stable but not phosphorylated and had a reduced activity of ∼33% in comparison to wild-type PTPS. Extracts from several human cell lines, including brain, contained a kinase that bound to and phosphorylated immobilized wild-type, but not mutant PTPS. Addition of cGMP stimulated phosphotransferase activity 2-fold. Extracts from transfected COS-1 cells overexpressing cGKII stimulated Ser19 phosphorylation more than 100-fold, but only 4-fold from cGKI overexpressing cells. Moreover, fibroblast extracts from mice lacking cGKII exhibited significantly reduced phosphorylation of PTPS. These results suggest that Ser19 of human PTPS may be a substrate for cGKII phosphorylation also in vivo, a modification that is essential for normal activity.
CLRG ChemTMiner @ Biocreative V

This paper describes our system developed for Biocreative V Chemical entity mention in patent (CEMP) task for identifying the chemical mentions from patent data. We have presented two systems which use Support vector machines (SVMs) and Conditional random fields (CRFs) algorithms. In this work we used a rich feature set that includes linguistic, orthographical and lexical clue features. We obtained an F-score of 0.817 using CRFs and F-score of 0.877 using SVMs on the development data. And on test data we obtained an Fscore of 0.7532, 0.74523 for SVM and CRFs respectively.
Fibromuscular dysplasia: Advances in understanding and management

Several key findings in recent years have reshaped our understanding of fibromuscular dysplasia (FMD), an uncommon nonatherosclerotic disease of medium-sized arteries that affects mainly women. While the true prevalence of this disease remains unknown, studies suggest that more people may be affected than previously reported. Better understanding of the clinical manifestations and natural history of FMD and advances in diagnostic imaging have altered the clinical approach to managing patients with this uncommon vascular disease. Although there are a multitude of unanswered questions regarding FMD, this review highlights recent insights and how this information has modified clinical care for those affected.
Incidence of Virulence Determinants and Antibiotic Resistance in Lactic Acid Bacteria Isolated from Iranian Traditional Fermented Camel Milk (Chal)

Lactic acid bacteria, including lactobacilli, enterococci, leuconostoc and weissella species isolated from Iranian traditional fermented camel milk (Chal) were assessed for the incidence of virulence determinants (gelE, efaAfm, efaAfs, ace, espfs, cylM, cylA and cylB), sensitivity to various antibiotics and virulence phenotypes. The incidence of virulence genes was determined by polymerase chain reaction and antibiotic susceptibility was tested by disk diffusion method. The results of this study indicated that all of the strains harbor at least two or more of the virulence genes. The most frequent virulence genes detected among tested strains were cylB, gelE and efaAfs. All strains showed no β-hemolysis while tyrosine decarboxylase activity and gelatinase production were observed in enterococcus and leuconostoc strains. Majority of strains were resistant to Polymyxin B and kanamycin. Lactobacillus strains including L. paraplantarum, L. kefiri, L. paracasei, L. plantarum and Weissella cibaria were resistant to both vancomycin and kanamycin. The possibility of transferring the antibiotic resistance and virulence genes to other starter and commensal strains makes the usage of these strains in food and dairy products controversial without required safety assessments.
Modelling, design, and testing of an electromagnetic power generator optimized for integration into shoes

Modelling and analysis of an electromagnetic generator designed for harvesting power produced during walking is presented. The generator is designed to be unobtrusive to the user by embedding it within the thickness of a normal shoe sole, and by applying a passive generation principle which requires minimal additional force over that normally exerted by the user during walking. In this way, a portion of the power used in walking is harvested for potential use in powering portable electronic devices. Analysis of different geometrical and material properties is applied to identify conditions for optimized designs; average power levels of up to 14 mW (200 mW of instantaneous power) are demonstrated within a volume of 15 × 15 × 100 mm3 at a walking speed of 2 steps per second. At least two of these volumes can be easily accommodated within a shoe heel to provide up to a 56 mW average power per user, and higher power levels can be achieved for faster walking or running speeds.
Long-term outcome in anorexia nervosa in the community.

OBJECTIVE Few studies have assessed outcomes of anorexia nervosa (AN) outside clinical settings. We aimed to assess mortality, recovery, and socio-demographic outcomes of AN in a community sample. METHOD Women in the nationwide FinnTwin16 cohort (born 1975-1979) were followed for 10 years after baseline diagnostic assessment (mean age at follow-up 34 years, N = 2188). We compared women with lifetime DSM-IV AN (N = 40) with unaffected women from the same cohort. RESULTS None of the women with AN had died and 88% were weight-recovered (BMI ≥ 18.5 kg/m(2) ), but their mean BMI (22.0 kg/m(2) ) was lower than among unaffected women (24.0 kg/m(2) , p = 0.008). University degrees (38 vs. 29%, p = 0.26), sickness absence during the past year (median 5 vs. 3 days, p = 0.21), or unemployment or disability pension (5 vs. 4%, p = 0.62) did not significantly differ between AN probands and their unaffected peers. More women with AN were still studying (15 vs. 4%, p = 0.003), and half of them had children, as compared to 66% of unaffected women (p = 0.05). DISCUSSION The long-term prognosis of AN in the community appears promising. Weight-restoration is common and socio-demographic outcomes are generally favorable. However, women with a history of AN may be less likely to have children.
A Redescription of Entamoeba Histolytica Schaudinn, 1903 (Emended Walker, 1911) Separating It From Entamoeba Dispar Brumpt, 1925 1

ABSTRACT. Explaining the low incidence of invasive disease (10%) in humans infected with Entamoeba histolytica has occupied the attention of generations of both clinical and nonclinical investigators. One possible explanation would be the existence of two morphologically identical species—one an invasive pathogen, the other noninvasive. This was first proposed by Brumpt in 1925, but his explanation was virtually ignored until 1978 when the first of several publications appeared suggesting that E. histolytica did indeed consist of two species. We have reexamined Brumpt's claim in light of recent biochemical, immunological and genetic studies and conclude that the data derived from these investigations provide unequivocal evidence supporting his hypothesis. With this in mind, we redescribe the invasive parasite retaining the name Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911), and set it apart from the noninvasive parasite described by Brumpt, Entamoeba dispar Brumpt, 1925.
Limb reduction anomaly after failed misoprostol abortion.

A 42-year-old woman, para 5, gravida 6, was admitted to Coronation Hospital on 2 June 1997. She reported vaginal bleeding for the past month and draining of amniotic fluid for 2 days. She had had 5 normal deliveries, the last having been of twins, and her 6 children ranged in age from 8 years to 18 years. Her last menstrual period had been on 25 November 1996. She, her husband and 3 of her children had been born with an extra digit on one or both hands. The past medical history waS otherwise non-contributory. The patient gave the following history relevant to the current pregnancy. In March 1997 pregnancy had been diagnosed by a general practitioner, and she had gone to her local hospital to request termination of pregnancy in terms of the newly promulgated Act. An ultrasound scan confirmed pregnancy of about 13 weeks' duration. Termination of the pregnancy was attempted with misoprostol tablets, one inserted vaginally on 4 consecutive days. On the 4th day some vaginal bleeding occurred. One week later she was admitted to the hospital for sterilisation. A minilaparotomy was performed under general anaesthesia, and after the operation she was informed that the sterilisation had not been performed because she had been found to still be pregnant. She had decided not to make any further attempt at termination of the pregnancy. On the patient's admission to Coronation Hospital on 2 June, her general condition was found to be good. The uterus was soft and non-tender and the symphysis-fundus measurement was 24 cm. Vaginal examination with a speculum confirmed drainage of non-offensive amniotic fluid and slight bleeding. Ultrasound examination revealed a breech presentation with measurements corresponding to 26 weeks' gestation, an estimated fetal weight of 970 g and greatly reduced amniotic fluid volume, which made detailed assessment for fetal anomalies difficult. The patient was counselled about the risks of ruptured membranes at this gestational age, and the option of induction of labour, but she requested conservative management. She was treated with prophylactic antibiotics and steroid therapy to promote fetal lung maturity. Her
A New Charge-Mosaic Membrane from a Multiblock Copolymer

A charge-mosaic membrane was prepared from a pentablock copolymer of the BABCB type by selectively introducing anion and cation exchange groups into the microseparated phases. The three-layer lamellar structure of the starting pentablock copolymer film was not disturbed by the modifications. The membrane obtained was highly permeable only to sodium chloride in mixed aqueous solutions of sodium chloride and organic species of low molecular weight, such as sucrose. Marked pH-dependent permeabilities were also observed for amino acids.
A Framework for QoE-Aware 3D Video Streaming Optimisation over Wireless Networks

The delivery of three-dimensional immersive media to individual users remains a highly challenging problem due to the large amount of data involved, diverse network characteristics, and user terminal requirements, as well as user’s context. This paper proposes a framework for quality of experience-aware delivering of three-dimensional video across heterogeneous wireless networks. The proposed architecture combines a Media-Aware Proxy (application layer filter), an enhanced version of IEEE 802.21 protocol for monitoring key performance parameters from different entities and multiple layers, and a QoE controller with a machine learning-based decision engine, capable of modelling the perceived video quality. The proposed architecture is fully integrated with the Long Term Evolution Enhanced Packet Core networks. The paper investigates machine learning-based techniques for producing an objective QoE model based on parameters from the physical, the data link, and the network layers. Extensive test-bed experiments and statistical analysis indicate that the proposed framework is capable of modelling accurately the impact of network impairments to the perceptual quality of three-dimensional video user.
Why do they stay? Job tenure among certified nursing assistants in nursing homes.

PURPOSE This study identifies factors related to job tenure among certified nursing assistants (CNAs) working in nursing homes. DESIGN AND METHODS The study uses 2004 data from the National Nursing Home Survey, the National Nursing Assistant Survey, and the Area Resource File. Ordinary least squares regression analyses were conducted with length of job tenure as the dependent variable. Tenure of CNAs was hypothesized to be motivated by the extrinsic rewards of their job, initial training and mentoring, reasons for being a CNA, organizational culture, and personal, facility, and market characteristics. Separate analyses were conducted for the overall sample and for CNAs who worked for the facility for more than 1 year. RESULTS Among policy-relevant domains, extrinsic rewards had the largest number of significant variables (4). Only 1 training and 1 organizational culture variable significantly affected CNA job tenure. Significant variables in domains not readily influenced by policy (e.g., personal characteristics and characteristics of the facility and surrounding market area) were often significant in both regressions. IMPLICATIONS This study underscores the importance of the basic economics of job choice by low-income workers. Wages, fringe benefits, job security, and alternative choices of employment are important determinants of job tenure that should be addressed, in addition to training and organizational culture.
VEGF-A AND -C but not -B mediate increased vascular permeability in preserved lung grafts1

Background. Vascular endothelial growth factor (VEGF) is a potent endothelial cell growth and permeability factor, expressed in the lung. Overexpression of VEGF is associated with increased vascular permeability in the early stage of acute lung injury in mice. The role of various forms of VEGF in transplantation-induced lung injury is not well understood. Methods. VEGF mRNA and protein expression was measured in biopsies of preserved donor lung grafts as well as in control lung biopsies, using real-time reverse transcriptase–polymerase chain reaction and Western blot analysis. VEGF tissue expression was also evaluated by immunocytochemistry. Serum VEGF was measured in recipients after transplantation and in controls using ELISA. Results. Although VEGF-A and VEGF-C protein expression was up-regulated, their mRNA levels were decreased in donor versus control lung biopsies (P <0.05). VEGF-B mRNA was decreased, but its protein level was unchanged in donors. Flt-1 was unchanged, KDR gene expression was down-regulated in donors (P <0.05), and both receptors’ protein expression was under the detection level in donor and control lungs. VEGF-A was detected in pulmonary vessels and bronchi, whereas VEGF-C was only detectable in vessels of both donor and control lungs. After transplantation, serum VEGF increased (P <0.05) and returned to control baseline levels 12 weeks after surgery. Wet-to-dry lung weight was increased in donor versus control lungs. Conclusions. These results indicate that unventilated hypoxia increases vascular permeability in lung grafts and that this process is mainly regulated at VEGF-A and VEGF-C translational but not transcriptional level. Selective VEGF antagonism during graft preservation might be of benefit to counteract edema formation.
Locking Timestamps versus Locking Objects

We present multiversion timestamp locking (MVTL), a new genre of multiversion concurrency control algorithms for serializable transactions. The key idea behind MVTL is simple: lock individual timestamps instead of locking objects. After presenting a generic MVTL algorithm, we demonstrate MVTL's expressiveness: we give several simple MVTL algorithms that address limitations of current multiversion schemes, by committing transactions that previous schemes would abort, by avoiding the problems of serial aborts or ghost aborts, and by offering a way to prioritize transactions that should not be aborted. We give evidence that, in practice, MVTL-based algorithms can outperform alternative concurrency control schemes.
Apprentissage de conversion de documents semi-structurés à partir d'exemples

We propose here the method called ISM -Incremental Structur e Mapping- which al- lows one to convert XML documents from heterogeneous source s to a mediated schema. Unlike existing methods, ISM is document centric and takes into acc ount both the structural informa- tion and the content information. It does not need to specifymanually correspondences between schema and is based on Machine Learning methods in order to tr documents to the me- diated schema. The method learns the transformation using aset of documents expressed in both the input schema and the output schema. At last, ISM has alow complexity and can be used with very large XML collections. This methods is experi enced here on a set of corpus and different tasks. The results show that the algorithm is ableto learn complex transformations and to transform large corpora, particularly for the conversio n of HTML documents to semantically rich XML documents.
Increased variability of the Arctic summer ice extent in a warmer climate

Simulations performed with general circulation models and a model of intermediate complexity show that the variability of the September sea ice extent in the Arctic of the 21st century increases first when the mean extent decreases from present‐day values. A maximum of the variance is found when the mean September ice extent is around 3 million km2. For lower extents, the variance declines with the mean extent. The behavior is clearly different in Antarctica where the variance always decreases as the mean ice extent decreases, following roughly a square‐root law compatible with very simple geometric arguments. Several mechanisms are responsible for the non‐linear behavior of the Arctic. However, the strong interhemispheric contrast suggests that the difference in geometrical setting, with an open ocean in the south and a semi‐closed basin in the north, plays a significant role.
Human papilloma virus screening by hybrid capture II in Chinese women of Jiangsu Province.

OBJECTIVE To survey Human Papilloma Virus (HPV) infection in Chinese Women of Jiangsu Province and discuss the relationship between HPV and the biology of cervical cancer. METHODS Two thousand, one hundred and fifty-three sexually active women (including 66 cases of cervical cancer) were selected for high-risk human papilloma virus DNA test with Hybrid Capture II (HCII). RESULTS The overall HPV prevalence was 32.6% (701/2153) with higher positive rates in cervical carcinoma and Cervical Interstitial Neoplasia (CIN) [93.9% and 54.6%] respectively. For women aged 40-59 years, the overall high-risk HPV prevalence was higher than those of other age groups. Compared with CIN I, the positivity rate and viral load of HPV DNA in CIN III is much higher (80.2% vs. 29.9%, 11.89 vs. 0.53). Ninety-four per cent (64/66) of patients with Cervical cancer were detected to be HPV positive. There was no significant difference in HPV DNA among each clinical stage and pathologic grade. But the positive rates and the value of HPV DNA were higher in the patients with cervical interstitial incursion. Eighty per cent of patients (20/25) could become negative within six months after operation. CONCLUSIONS High-risk HPV DNA test is effective in screening for cervical diseases. HCII is an effective method to detect HPV DNA.
Decentralization of BPEL Using Various Processes

This article presents BPELcube, a framework comprising a scalable architecture and a set of distributed algorithms, which support the decentralized enactment of BPEL processes. In many application domains, BPEL processes are longrunning, they involve the exchange of voluminous data with external Web services, and are concurrently accessed by large numbers of users. In such context, centralized BPEL process execution engines pose considerable limitations in terms of scalability and performance.To overcome such problems, a scalable hypercube peer-to-peer topology is employed by BPELcube in order to organize an arbitrar number of nodes, which can then collaborate in the decentralized execution and monitoring of BPEL processes. Contrary to traditional clustering approaches, each node does not fully take charge of executing the whole process; rather, it contributes to the overall process execution by running a subset of the process activities, and maintaining a subset of the process variables. Hence, the hypercubebased infrastructure acts as a single execution engine, where workload is evenly distributed among the participating nodes in a finegrained manner. An experimental evaluation of BPELcube and a comparison with centralized and clustered BPEL engine architectures demonstrates that the decentralized approach yields improved process execution times and throughput
New uses for phenytoin.

PHENYTOIN (diphenylhydantoin) is a clinically accepted therapeutic agent for seizure control, for the treatment of cardiac arrhythmias, and in the diagnosis (and treatment) of certain endocrine diseases. In addition, however, phenytoin has been reported to be of value in treating at least 75 different symptoms and disorders. Bogoch and Dreyfus 1 have reported these various clinical uses in their book, The Broad Range of Use of Diphenylhydantoin . As pointed out in the Medical Letter on Drugs and Therapeutics , the book lists a total of 75 symptoms and disorders, from abdominal pain to wounds, in which phenytoin is reported to be valuable. The bibliography is of interest, but the reviews of the uses of the drug are too uncritical to be of much value. Of the 750 articles mentioned, only 17 studies are described as being controlled to any extent. The majority of these proposed uses are supported only by anecdotal evidence.