Miracle-dz/UniFAR-FTUs huggingface dataset:
Alternative mental health clinical placements: Knowledge transfer and benefits for nursing practice outside mental health care settings.

AIM AND OBJECTIVES To explore whether nursing student's experiences at Recovery Camp have impacted their current nursing practices. BACKGROUND Recently, there has been a move toward more holistic models of nursing care, which seek to break down barriers of stigmatisation and embrace the tenets of self-determination, to acknowledge people with lived experiences of mental illness and their ability to manage their recovery. In that regard, future health professionals such as nursing students, will need to be educated in a manner that recognises the importance of lived experience. In this paper, we propose that Recovery Camp, an alternative clinical placement setting model, enhances clinical practice in multiple domains and is beneficial for both nursing practitioners and people with lived experiences of mental illness, as well as offering an effective non-traditional alternative to conventional clinical placement opportunities. METHODS This study employed a phenomenological research design, involving individual semi-structured telephone interviews. The Standards for Reporting Qualitative Research (SRQR) checklist was adhered to. RESULTS Three main themes were identified from the analysis: 1) engagement, 2) understanding mental health and 3) holistic care. "I definitely look at people with mental health conditions in a different light". At Recovery Camp, participants felt that they had greater opportunities for engagement with people with lived experiences, and through this engagement their preconceptions of mental illness began to change. CONCLUSIONS Recovery Camp may have facilitated the transfer of knowledge that is more person-centred among nursing students, consequently impacting their current nursing practices. RELEVANCE TO CLINICAL PRACTICE Nurses should be equipped with mental health skills regardless of their career trajectory. While Recovery Camp represents a promising approach to facilitate knowledge transfer, further investigation will be required to determine which other factors are instrumental. This approach may have wider implications for nursing education.
Estimating the impact of HIV combination prevention in men who have sex with men, the Bangkok MSM Cohort Study, Thailand

In Thailand, pre-exposure prophylaxis (PrEP) is recommended for human immunodeficiency virus (HIV) prevention among at-risk men who have sex with men (MSM). We modeled the impact of PrEP and condom use as independent and combined interventions on the estimated number of HIV infections among a hypothetical population of 10,000 MSM in Bangkok, Thailand. Our model demonstrated a 92% (95% confidence interval 89.7, 94.2) reduction in HIV infections among Thai MSM who took daily PrEP and self-reported using condoms correctly and consistently (100% condom use). Increased use of PrEP and condoms likely would have a substantial impact on the HIV epidemic in Thailand.
Cyanine-like Boronic Acid Derived Salicylidenehydrazone Complexes (Cy-BASHY) for Bioimaging Applications.

BASHY dyes with a polymethine backbone were desgined to yield efficient red-emitting and two-photon absorbing fluorophores that can be used as markers for astrocytes. The dyes are chemically stable in aqueous solution and do not undergo photodecomposition. Their photophysical properties can be electronically fine-tuned and thereby adapted to potentially different imaging situations and requierements.
Optimum roughage proportion in barley-based feedlot cattle diets: Total-tract nutrient digestibility, rumination, ruminal acidosis, short-chain fatty absorption, and gastrointestinal tract barrier function.

Cattle need physically effective fiber to promote rumination and maintain rumen health, but economics favor the use of low-roughage feedlot diets. The study investigated the optimum barley silage proportion in barley-based finishing diets. Apparent total-tract digestibility (4-d total fecal collection), chewing behavior (6-d video recording), ruminal pH (6-d indwelling pH recording) and fermentation (1 day, sampling 0, 3, 6, 12, and 18 h post-feeding), short-chain fatty acid (SCFA) absorption (washed reticulo-rumen technique), gastrointestinal tract barrier function (marker infusion), and blood variables (catheters) were measured. Eight ruminally-fistulated crossbred beef heifers (653 ± 44.2 kg; mean starting body weight ± SD) were used in a replicated 4 × 4 Latin square design with 28-d periods. Dietary treatments were 0, 4, 8, and 12% of dietary DM as barley silage, with diets containing 80, 76, 72, and 68% barley grain, respectively. Increasing silage proportion decreased dietary starch content from 49.0 to 43.1% DM, while neutral detergent content increased from 22.7 to 25.1% DM. Silage proportion had no effect on DM intake, but apparent DM digestibility decreased quadratically (86.0, 82.1, 81.1, 79.5% for the 4 diets, respectively; P < 0.001). Although, silage proportion had no effect on eating activity, rumination time increased quadratically (246, 289, 302, 316 min/d; P = 0.04). Increased silage proportion increased minimum (5.07, 5.27, 5.29, 5.41; quadratic, P = 0.011) and mean (5.61, 5.87, 5.93, 5.95; quadratic, P = 0.007) ruminal pH, and there was a quadratic (P ≤ 0.047) decrease in duration and area under the pH acidosis threshold curves of 5.8, 5.5, and 5.2. Although increasing silage proportion decreased ruminal acidosis, it was not completely eliminated even with a diet containing 12% silage DM. Short chain fatty acid concentration in ruminal fluid was not affected by diet, but silage proportion quadratically (P ≤ 0.088) increased ruminal acetate:propionate. There was no effect of diet on absolute or fractional rates of absorption of acetate, propionate, butyrate or total SCFA, and no effect on gastrointestinal barrier function or blood measurements. In conclusion, responses to roughage level were mostly quadratic with greatest improvements in acidosis variables between 0 and 4% barley silage, with incremental improvements with further increases in silage levels. The study showed a trade-off between maximizing digestibility and energy intake to promote animal performance and minimizing the risk of acidosis.
Return to Sport following Isolated Lateral Opening Wedge Distal Femoral Osteotomy

Purpose. The aims of this study were to (1) examine the timeline of return to sport (RTS) following isolated lateral opening wedge distal femoral osteotomy (DFO), (2) evaluate the degree of participation on RTS, and (3) identify risk factors for failure to RTS. Methods. Nineteen consecutive patients undergoing isolated lateral opening wedge DFO were reviewed retrospectively at a minimum of 2 years postoperatively. Patients completed a sports questionnaire, visual analogue scale for pain (VAS-Pain), Single Assessment Numerical Evaluation (SANE), and a satisfaction questionnaire. Results. Seventeen patents (89.5%; age 32.1 ± 10.1 years; gender 9 males, 52.9%) were contacted at 7.3 ± 4.4 years (range 2.0-13.8 years). Twelve patients (70.6%) resumed playing ≥1 sport at an average time of 9.5 ± 3.3 months (range 3-12 months). Of these 12 patients, 6 returned to a lower level of participation (50.0%). Seven patients (41.2%) had returned to the operating room for further surgery, which included removal of hardware (5.9%) and total knee arthroplasty (5.9%). The average VAS-Pain, SANE, and Marx scores were 3.4 ± 2.6 (range 0-8), 56.2 ± 18.7 (range 20-85), and 5.0 ± 5.3 (range 0-16), respectively. Fourteen patients (82.4%) were at least somewhat satisfied with their procedure. Conclusion. In patients with isolated lateral compartment osteoarthritis and valgus deformity, lateral opening wedge DFO allows 70.6% of patients to RTS by 9.5 ± 3.3 months. However, most patients may be unable to return to their presymptomatic level of function. Patient expectations regarding RTS can be appropriately managed with adequate preoperative patient education. Level of Evidence. IV, case series.
Multicopper oxidases: modular structure, sequence space, and evolutionary relationships

Multicopper oxidases (MCOs) use copper ions as cofactors to oxidize a variety of substrates while reducing oxygen to water. MCOs have been identified in various taxa, with notable occurrences in fungi. The role of these fungal MCOs in lignin degradation sparked an interest due to their potential for application in biofuel production and various other industries. MCOs consist of different protein domains, which led to their classification into two‐, three‐, and six‐domain MCOs. The previously established Laccase and Multicopper Oxidase Engineering Database (https://lcced.biocatnet.de) was updated and now includes 51 058 sequences and 229 structures of MCOs. Sequences and structures of all MCOs were systematically compared. All MCOs consist of cupredoxin‐like domains. Two‐domain MCOs are formed by the N‐ and C‐terminal domain (domain N and C), while three‐domain MCOs have an additional domain (M) in between, homologous to domain C. The six‐domain MCOs consist of alternating domains N and C, each three times. Two standard numbering schemes were developed for the copper‐binding domains N and C, which facilitated the identification of conserved positions and a comparison to previously reported results from mutagenesis studies. Two sequence motifs for the copper binding sites were identified per domain. Their modularity, depending on the placement of the T1‐copper binding site, was demonstrated. Protein sequence networks showed relationships between two‐ and three‐domain MCOs, allowing for family‐specific annotation and inference of evolutionary relationships.
QTLs for Shoot Length and Chlorophyll Content of Rice Seedlings Grown under Low-Temperature Conditions, using a Cross between Indica and Japonica Cultivars

Abstract Quantitative trait loci (QTLs) affecting the shoot length and chlorophyll content of seedlings grown at a low temperature (16ºC) for 4 weeks were analyzed using inbred lines derived from a cross between an indica rice cultivar, Habataki, and a japonica rice cultivar, Arroz da Terra. Culm length of the inbred lines, measured at the harvesting stage, was also analyzed. The QTLs linked to increased shoot length (qSL1 ) and culm length (qCL1 ) on the Arroz da Terra allele were detected in the same region of chromosome 1, where the gibberellin 20 oxidase-2 gene (identical to Semi Dwarf1 : SD1 ) located. Other QTLs ( qSL and qSL8 ) that were linked to increased shoot length on Arroz da Terra and Habataki alleles, but had no effects on the culm length, were detected on chromosomes 3 and 8, respectively. The alleles of qSL3 and qSL8 might improve early shoot growth without increasing the risk of lodging caused by a long culm length. A novel QTL (qCC1 ) linked to increased chlorophyll content of seedlings grown at low temperatures through the effect of an Arroz da Terra allele was detected on chromosome 1, but it differed in position from the QTLs for shoot length.
Internet-Based Intervention for Postpartum Depression in China ("Mommy go"): Protocol for a Randomized Controlled Trial.

AIM The purpose of this study is to design a research protocol for the clinical testing of the "Mommy go" for pregnant women with a risk of postpartum depression. DESIGN A non-blinded randomized controlled trial. METHODS A randomized controlled study will be performed from January 2018 to the completion of the study. The intervention group will follow the "Mommy go" protocol and the control group will receive traditional support. We will use the Edinburgh Postpartum Depression Scale and the Chinese version of the Postpartum Depression Predictors Inventory-Revised to measure the risk of postpartum depression in pregnant women. The outcomes are clinical data, postpartum depressive mood, self-efficacy and infant temperament. Outcomes will be assessed using questionnaires and through data generated by digital technologies. DISCUSSION The expected outcomes are increased self-efficacy and infant temperament, reduced postpartum depressive mood and improvements to postpartum depression. We expect the study to have a clinical impact on future online interventions for postpartum depression in China. IMPACT This study will provide an internet-based intervention for postpartum depression in China. It will be implemented in clinical practice if it can effectively improve postpartum depression.
Neural and psychophysiological correlates of social communication development: Evidence from sensory processing, motor, cognitive, language and emotional behavioral milestones across infancy

Abstract This article presents a literature review focusing on the neural and psychophysiological correlates associated with social communication development in infancy. Studies presenting evidence on infants’ brain activity and developments in infant sensory processing, motor, cognitive, language, and emotional abilities are described in regard to the neuropsychophysiological processes underlying the emergence of these specific behavioral milestones and their associations with social communication development. Studies that consider specific age-related characteristics across the infancy period are presented. Evidence suggests that specific neural and physiological signatures accompany age-related social communication development during the first 18 months of life.
Combination therapy of oxidised regenerated cellulose/collagen/silver dressings with negative pressure wound therapy for coverage of exposed critical structures in complex lower‐extremity wounds

Complex wounds with exposed critical structures such as tendon and bone are a conundrum in wound management, especially in the setting where the patient is not a suitable candidate for flap surgery. While the individual use of negative pressure wound therapy (NPWT) and oxidised regenerated cellulose (ORC)/collagen/silver (PROMOGRAN PRISMA) dressing has been described in the literature, there are little data on the efficacy of their combined use. In this study, we describe a novel technique of combining the use of NPWT and ORC/collagen/silver dressings to manage complex wound beds as an alternative management option for patients not suitable for reconstructive flap surgery. This technique was performed in a series of 37 patients with complex lower‐extremity wounds that were not healing with conventional NPWT alone. All patients had open wounds with exposed critical structures that were difficult to manage, such as exposed tendon, bone, deep crevices, and joint. Successful coverage of exposed critical structures was achieved in 89% of patients, and coverage was achieved within 28 days of combination therapy in 82% of these patients, without any complications. The novel technique of combining ORC/collagen/silver dressing and NPWT provides a useful option in the armamentarium of a reconstructive surgeon dealing with difficult complex lower‐extremity wounds.
Paired Basic Amino Acid-cleaving Enzyme 4 (PCSK6): An Emerging New Target Molecule in Human Melanoma

Although recent therapeutic developments raise hope, melanoma remains a devastating disease with a need for new treatment targets. In other tumours prohormone convertases have been shown to be pro-tumourigenic as they are involved in processing preforms of matrix-metalloproteinases, growth factors and adhesion molecules. The aim of this study was to look for new treatment options for melanoma, by investigating the role of the prohormone convertase Paired basic Amino acid-Cleaving Enzyme 4 (PACE4/PCSK6) in melanoma cell lines and human melanoma tissue. PACE4-transfected A375 melanoma cells displayed significantly increased proliferation, MMP-2 production, gelatinase activity and migratory capacity in vitro compared with sham-transfected cells. In vivo, elevated PACE4 expression resulted in significantly increased tumour growth on immunodeficient mice. In the majority of 45 human primary melanomas and melanoma metastases ex vivo PACE4 immunoreactivity was detectable, while it was absent in in situ melanomas. These results indicate PACE4 as a regulator of melanoma cell aggressiveness.
New antimicrobial and cytotoxic benzofuran glucoside from Senecio glaucus L

Abstract The ethyl acetate fraction of the dried aerial parts of Senecio glaucus L. exhibited significant antimicrobial activity against some of selected bacteria and fungi. Also, it showed potent cytotoxicity against PANC-1 cancer cell lines under glucose deficient medium. The ethyl acetate fraction was subjected to different chromatographic techniques for isolation of the bioactive compounds. A new benzofuran glucoside; 2,3-dihydro-3β-hydroxyeuparin 3-O-glucopyranoside (1) was isolated. Additionally, two known flavonoid compounds isorhamentin 3-O-β-D-glucoside (2), and isorhamentin 3-O-β-D-rutinoside (3) were first identified in S. glaucus. Compound 1 exhibited potent antimicrobial activities against two Gram-positive bacteria, one Gram-negative bacteria, and two fungi. Also, it displayed potent cytotoxic activity against PANC-1 cancer cell lines under glucose deficient medium (IC50 7.5 μM). However, the isolated flavonoid glycosides (2 & 3) showed moderate antimicrobial activities against two Gram-positive bacteria, two Gram-negative bacteria, four fungi, and did not show any cytotoxic activity against PANC-1. Graphical Abstract
Recent Advances and Applications of Microfluidic Capillary Electrophoresis: A Comprehensive Review (2017–Mid 2019)

Abstract Microfluidic capillary electrophoresis (MCE) is the novel technique resulted from the CE mininaturization as planar separation and analysis device. This review presents and discusses various application fields of this advanced technology published in the period 2017 till mid-2019 in eight different sections including clinical, biological, single cell analysis, environmental, pharmaceuticals, food analysis, forensic and ion analysis. The need for miniaturization of CE and the consequence advantages achieved are also discussed including high-throughput, miniaturized detection, effective separation, portability and the need for micro- or even nano-volume of samples. Comprehensive tables for the MCE applications in the different studied fields are provided. Also, figure comparing the number of the published papers applying MCE in the eight discussed fields within the studied period is included. The future investigation should put into consideration the possibility of replacing conventional CE with the MCE after proper validation. Suitable validation parameters with their suitable accepted ranges should be tailored for analysis methods utilizing such unique technique (MCE).
Effects of Chelated Copper and Zinc Supplementation on Growth Performance, Nutrient Digestibility, Blood Profiles, and Fecal Noxious Gas Emission in Weanling Pigs

ABSTRACT This study was conducted to evaluate the effects of chelated Cu and Zn on growth performance, nutrient digestibility, blood profiles, and fecal noxious gas emission in weanling pigs. A total of 90 weanling pigs with an initial body weight (BW) of 5.27 ± 0.04 kg were randomly allotted to two dietary treatments for 42 d. Pigs were then fed a control diet (CON) or a Zinpro diet (CON + 0.1% chelate copper and zinc). There were nine replicate-pens with five pigs in each pen. During d 0 to 14 and d 14 to 28, the ADFI decreased ( p <0.05) and the G/F increased ( p <0.05) in pigs fed the Zinpro diet compared with those that received the CON diet. During d 28 to 42, the ADFI increased ( p <0.05) in pigs fed the Zinpro diet relative to those fed the CON diet. Additionally, the apparent total tract digestibility of DM, N, and energy increased ( p <0.05) in the Zinpro group when compared to the CON group on d 14 and 28. The lymphocyte percentage was also greater ( p <0.05) in the Zinpro group than in the CON group. Overall, dietary supplementation with 0.1% chelate copper and zinc improved the growth performance and nutrient digestibility in weanling pigs. (
Daucosterol from Crateva adansonii DC (Capparaceae) reduces 7,12‐dimethylbenz(a)anthracene‐induced mammary tumors in Wistar rats

This study aimed to evaluate the in vivo anticancer effects of daucosterol which was earlier reported to possess in vitro anticancer effects. Breast tumor was induced in 30 rats using the environmental carcinogen 7,12‐dimethylbenz(a)anthracene (DMBA) while 6 control rats received olive oil (NOR). Animals with palpable tumors were randomized into five groups (n = 6) each as follows: negative control group treated with the vehicle (DMBA); positive control group treated with 5 mg/kg BW doxorubicin (DOXO + DMBA); three groups treated with daucosterol at doses of 2.5, 5, and 10 mg/kg BW (DAU + DMBA). Treatment lasted 28 days afterward, tumor (mass, volume, cancer antigen [CA] 15‐3 level and histoarchitecture), hematological and toxicological parameters were examined. The tumor volume gradually increased in the DMBA group during the 28 days, with a tumor volume gain of ∼390 cm3. Daucosterol at all doses reduced tumor volume (∼133.7 cm3 at 10 mg/kg) as well as protein, malondialdehyde (MDA), and CA 15‐3 levels compared to DMBA rats. Tumor sections in daucosterol‐treated rats showed a lower proliferation of mammary ducts with mild (5 and 10 mg/kg) to moderate (2.5 mg/kg) inflammatory responses. Moreover, it exhibited an antioxidant effect, evidenced by a significant and dose‐dependent decreased in MDA levels, as well as an increase in catalase activity compared to the DMBA group. Daucosterol showed for the first time in vivo antitumor effects that corroborate its previous in vitro effects.
Environmental contaminants and male infertility: Effects and mechanisms

The escalating prevalence of male infertility and decreasing trend in sperm quality have been correlated with rapid industrialisation and the associated discharge of an excess of synthetic substances into the environment. Humans are inevitably exposed to these ubiquitously distributed environmental contaminants, which possess the ability to intervene with the growth and function of male reproductive organs. Several epidemiological reports have correlated the blood and seminal levels of environmental contaminants with poor sperm quality. Numerous in vivo and in vitro studies have been conducted to investigate the effect of various environmental contaminants on spermatogenesis, steroidogenesis, Sertoli cells, blood–testis barrier, epididymis and sperm functions. The reported reprotoxic effects include alterations in the spermatogenic cycle, increased germ cell apoptosis, inhibition of steroidogenesis, decreased Leydig cell viability, impairment of Sertoli cell structure and function, altered expression of steroid receptors, increased permeability of blood–testis barrier, induction of peroxidative and epigenetic alterations in spermatozoa resulting in poor sperm quality and function. In light of recent scientific reports, this review discusses the effects of environmental contaminants on the male reproductive function and the possible mechanisms of action.
Comparing three body condition indices in amphibians: a case study of yellow-bellied toad Bombina variegata

Body condition is important because it is correlated with population and habitat quality parameters. Since the direct measurements are either lethal or unreliable, a wide range of non-lethal body condition indices has been proposed. The aim of our study was to apply and compare three body condition indices (Fulton's index, relative body condition mass index and residual index) using body size indicator - body mass data for 24 populations of the yellow bellied toad (Bombina variegata). The condition index should be independent of body size indicator, in this case snout vent length (SVL). Therefore we tested all three indices for the statistical independence of SVL and for the normality of distribution. Fulton's index violated the independence assumption, whereas the relative body condition mass index did not have a normal distribution. Residual index was found both independent of SVL and normally distributed. Moreover, the residual index highlighted biological significant differences on the basis of altitude and season. Our results recommend the residual index as a useful tool in amphibian monitoring and conservation.