Miracle-dz/UniFAR-FTUs huggingface dataset:
Localization of von Willebrand Factor and Thrombin-Interactive Domains on Human Platelet Glycoprotein Ib

Summary Platelet membrane glycoprotein Ib (GPIb) functions as receptors for thrombin and von Willebrand factor (vWF) in the presence of ristocetin. To precisely locate the domains on GPIb interacting with vWF and thrombin, we prepared several peptides that have amino acid sequences analogous to that ol the GPIb α-chain and examined their effects on ristocetin-induced (vWFdependent) and thrombin-induced platelet aggregations. A peptide extending from residues Asp235 to Lys262 showed the strongest inhibitory effect on ristocetin-induced platelet agglutination, and a group of overlapping peptides composed of 24-28 amino acid residues representing sequences extending from Phe216 to Asp274 was found to inhibit platelet aggregation induced by thrombin. Other peptides did not inhibit platelet aggregations. Moreover the binding to platelets of the monoclonal anti-GPIb antibody (TM60) which had been shown to inhibit both ristocetin- and thrombin-induced platelet aggregations was strongly inhibited by a peptide extending from Asp249 to Asp274. These data demonstrate that the vWF-hinding domain exists in a small region between residues Asp235 and Lys262; the thrombin-interacting domain, in contrast, is located between residues Phe216 and Ala274, with a possible center of interaction in the sequence from Phe216 to Thr240 on the GPIb α-chain, and thrombin binding requires a relatively strict conformation in this domain.
A magnetic shielding type superconducting fault current limiter using a Bi2212 thick film cylinder

The application of a superconductor to a fault current limiter in a power system has been researched. The authors developed a magnetic shielding type superconducting fault current limiter using a Bi2212 thick film cylinder. This limiter consists of a primary winding on a superconducting cylinder for generating an AC magnetic field, the cylinder for shielding the field, an iron core for magnetic members and a control ring for controlling fault currents. When a fault occurs, the superconductive cylinder quenches and a magnetic circuit is formed inside the cylinder. As a result, the limiter has a high impedance to limit the fault current. The present study shows the limiting and recovery characteristics of the fault current limiter. <<ETX>>
Fetal assessment before and after dosing with buprenorphine or methadone.

AIM To determine pre- and post-dosing effects of prenatal methadone compared to buprenorphine on fetal wellbeing. DESIGN A secondary analysis of data derived from the Maternal Opioid Treatment: Human Experimental Research (MOTHER) study, a double-blind, double-dummy, randomized clinical trial. SETTING Six United States sites and one European site that provided comprehensive opioid-dependence treatment to pregnant women. PARTICIPANTS Eighty-one of the 131 opioid-dependent pregnant women completing the MOTHER clinical trial, assessed between 31 and 33 weeks of gestation. MEASUREMENTS Two fetal assessments were conducted, once before and once after study medication dosing. Measures included mean fetal heart rate (FHR), number of FHR accelerations, FHR reactivity in the fetal non-stress test (NST) and biophysical profile (BPP) score. FINDINGS Significant group differences were found for number of FHR accelerations, non-reactive NST and BPP scores (all Ps < 0.05). There were no significant group differences before time of dosing. Significant decreases (all Ps < 0.05) occurred from pre- to post-dose assessment for mean FHR, FHR accelerations, reactive NST and fetal movement. The decrease in accelerations and reactive NST were significant only for fetuses in the methadone group, and this resulted in a significantly lower likelihood of a reactive NST compared to fetuses in the buprenorphine group. CONCLUSION Buprenorphine compared with methadone appears to result in less suppression of mean fetal heart rate, fetal heart rate reactivity and the biophysical profile score after medication dosing and these findings provide support for the relative safety of buprenorphine when fetal indices are considered as part of the complete risk-benefit ratio.
Optimal Resource Allocation in Multicast Device-to-Device Communications Underlaying LTE Networks

In this paper, we present a framework for resource allocations for multicast device-to-device (D2D) communications underlaying the uplink of a Long-Term Evolution (LTE) network. The objective is to maximize the sum throughput of active cellular users (CUs) and feasible D2D multicast groups in a cell, while meeting a certain signal-to-interference-plus-noise ratio (SINR) constraint for both the CUs and the D2D groups. We formulate the general problem of power and channel allocation as a mixed integer nonlinear programming (MINLP) problem, where one D2D group can reuse the channels of multiple CUs and where the channel of each CU can be reused by multiple D2D groups. Distinct from existing approaches in the literature, our formulation and solution methods provide an effective and flexible means to utilize radio resources in cellular networks and share them with multicast groups without causing harmful interference to each other. The MINLP problem is transformed so that it can be solved optimally by a variant of the generalized Bender decomposition method with provable convergence. A greedy algorithm and a low-complexity heuristic solution are then devised. The performance of all schemes is evaluated through extensive simulations. Numerical results demonstrate that the proposed greedy algorithm can achieve close-to-optimal performance and that the heuristic algorithm provides good performance, even though it is inferior than that of the greedy, with much lower complexity.
Torque Distribution-Based Range Extension Control System for Longitudinal Motion of Electric Vehicles by LTI Modeling With Generalized Frequency Variable

Electric vehicles (EVs) have been identified as alternatives to traditional engine vehicles because they have no tailpipe emissions and are cheaper to operate. However, limited mileage per charge partially prevents EVs from becoming popular. Although high-efficiency motors and large batteries can be employed to increase the cruising range per charge, pure control approaches for energy saving are more promising. To increase the cruising range of EVs equipped with front and rear in-wheel-motors, this paper proposes an optimal torque distribution algorithm for longitudinal motion by considering the transfer of weight between front and rear axles and motor losses. Then, by modeling an EV as a linear time-invariant (LTI) system with generalized frequency variables, a distributed control concept is proposed, in which part of the main controller's task is allocated to the local controllers. A stability criterion is established for the controller design, and the effectiveness of the proposed range extension control system is verified by both simulations and experiments.
MicroRNAs, wild-type and mutant p53: More questions than answers

The tumor suppressor p53 is a sequence-specific transcription factor that activates the expression of genes involved in apoptosis, cell cycle arrest and senescence. p53 can also inhibit gene expression and this effect is partly mediated by inducing several microRNAs (miRNAs). MiRNAs have emerged as a new class of regulators of the expression and function of eukaryotic genomes. Tumor suppressive or oncogenic functions have been attributed to some miRNAs. Recent studies have shown that p53 can alter the transcription of several miRNAs, and in some cases, it can also influence miRNA maturation. Conversely, miRNAs can also modulate the abundance and activity of p53 by direct or indirect mechanisms. Moreover, mutant p53 can actively repress the expression of some miRNAs that are activated by wild-type p53. In this review, we discuss recent evidences of this crosstalk between miRNAs and the p53 network and also highlight its implications in cancer.
Report summary - Mood and Anxiety Disorders in Canada, 2016.

Mood and Anxiety Disorders in Canada, 2016 is the first publication to include administrative health data from the Canadian Chronic Disease Surveillance System (CCDSS) for the national surveillance of mood and anxiety disorders among Canadians aged one year and older. It features nationally complete CCDSS data up to fiscal year 2009/10, as well as trend data spanning over a decade (1996/97 to 2009/10). The data presented in this report, and subsequent updates, can be accessed via the Public Health Agency of Canada's Chronic Disease Infobase Data Cubes at www.infobase. phac-aspc.gc. ca. Data Cubes are interactive databases that allow users to quickly create tables and graphs using their Web browser. The report demonstrates the Public Health Agency of Canada's commitment to improving data collection and reporting about mental illness, as recommended within Changing Directions, Changing Lives - The Mental Health Strategy for Canada.
The Impact of Synaptic Zn2+ Dynamics on Cognition and Its Decline

The basal levels of extracellular Zn2+ are in the range of low nanomolar concentrations and less attention has been paid to Zn2+, compared to Ca2+, for synaptic activity. However, extracellular Zn2+ is necessary for synaptic activity. The basal levels of extracellular zinc are age-dependently increased in the rat hippocampus, implying that the basal levels of extracellular Zn2+ are also increased age-dependently and that extracellular Zn2+ dynamics are linked with age-related cognitive function and dysfunction. In the hippocampus, the influx of extracellular Zn2+ into postsynaptic neurons, which is often linked with Zn2+ release from neuron terminals, is critical for cognitive activity via long-term potentiation (LTP). In contrast, the excess influx of extracellular Zn2+ into postsynaptic neurons induces cognitive decline. Interestingly, the excess influx of extracellular Zn2+ more readily occurs in aged dentate granule cells and intracellular Zn2+-buffering, which is assessed with ZnAF-2DA, is weakened in the aged dentate granule cells. Characteristics (easiness) of extracellular Zn2+ influx seem to be linked with the weakened intracellular Zn2+-buffering in the aged dentate gyrus. This paper deals with the impact of synaptic Zn2+ signaling on cognition and its decline in comparison with synaptic Ca2+ signaling.
Excess Postexercise Oxygen Consumption After High-Intensity and Sprint Interval Exercise, and Continuous Steady-State Exercise

Abstract Tucker, WJ, Angadi, SS, and Gaesser, GA. Excess postexercise oxygen consumption after high-intensity and sprint interval exercise, and continuous steady-state exercise. J Strength Cond Res 30(11): 3090–3097, 2016—Higher excess postexercise oxygen consumption (EPOC) after high-intensity interval exercise (HIE) and sprint interval exercise (SIE) may contribute to greater fat loss sometimes reported after interval training compared with continuous steady-state exercise (SSE) training. We compared EPOC after HIE, SIE, and SSE. Ten recreationally active men (age 24 ± 4 years) participated in this randomized crossover study. On separate days, subjects completed a resting control trial and 3 exercise conditions on a cycle ergometer: HIE (four 4-minute intervals at 95% peak heart rate (HRpeak), separated by 3 minutes of active recovery), SIE (six 30-second Wingate sprints, separated by 4 minutes of active recovery), and SSE (30 minutes at 80% of HRpeak). Oxygen consumption (V[Combining Dot Above]O2) was measured continuously during and for 3 hours after exercise. For all conditions, V[Combining Dot Above]O2 was higher than resting control only during the first hour postexercise. Although 3-hour EPOC and total net exercise energy expenditure (EE) after exercise were higher (p = 0.01) for SIE (22.0 ± 9.3 L; 110 ± 47 kcal) compared with SSE (12.8 ± 8.5 L; 64 ± 43 kcal), total (exercise + postexercise) net O2 consumed and net EE were greater (p = 0.03) for SSE (69.5 ± 18.4 L; 348 ± 92 kcal) than those for SIE (54.2 ± 12.0 L; 271 ± 60 kcal). Corresponding values for HIE were not significantly different from SSE or SIE. Excess postexercise oxygen consumption after SIE and HIE is unlikely to account for the greater fat loss per unit EE associated with SIE and HIE training reported in the literature.
Migraine in Thai Children: Prevalence in Junior High School Students

A cross-sectional study to determine the prevalence of migraine in seventh grade Thai students in 4 junior high schools in Bangkok, Thailand, according to the diagnostic criteria of the second edition of the Classification of Headache of the International Headache Society was conducted in July 2004. The study included a screening self-administered questionnaire and face-to-face interview with physical examination. The diagnosis of migraine was made and confirmed by 2 pediatric neurologists. All of 1789 students in participating schools completed the questionnaire. After 2 interviews, 248 students (13.8%) were diagnosed with migraine. The prevalence in girls was higher than that in boys (16.2% vs 11.7%). Migraine as
Up‐regulation of type I procollagen C‐proteinase enhancer protein messenger RNA in rats with CCl4‐induced liver fibrosis

Using a polyclonal antibody raised against a liver stellate cell (LSC) line derived from a rat CCl4‐cirrhotic liver, we isolated 14 clones from a complementary DNA library prepared with total RNA extracted from the same cell line, with nucleotide sequences homologous to that of the type I procollagen C‐proteinase enhancer protein (PCPE) gene. The longest PCPE insert of 1,530 base pairs contained an open reading frame coding for 468 amino acids. PCPE cDNA recognized by Northern blot a 1.7‐kilobase messenger RNA (mRNA) in total RNA extracted from freshly isolated and early passaged LSC, LSC lines derived from normal (NFSC) and cirrhotic (CFSC) rat livers, and various LSC clones derived from CFSC. The expression of PCPE mRNA was increased threefold in CFSC compared with NFSC. PCPE mRNA was not detected in total rat liver, freshly isolated hepatocytes, or endothelial or Kupffer cells. However, the expression of PCPE mRNA was induced in fibrotic livers of rats treated with CCl4. PCPE mRNA expression in LSC was up‐regulated by transforming growth factor β1 (TGF‐β1) and down‐regulated by tumor necrosis factor α (TNF‐α), similar to the changes in α1 (1) procollagen mRNA induced by these cytokines. PCPE was not detectable in liver biomatrix proteins obtained from normal liver. However, PCPE was increased in liver biomatrix proteins from cirrhotic livers and was proportional to the amount of collagen. These data suggest that PCPE may play an important role in the processing of type I collagen during liver fibrogenesis, and that TGF‐β1 and TNF‐α regulate its expression.
Quantitation of Depth of Thiopental Anesthesia in the Rat

Background In contrast to that of inhalational anesthetics, quantitation of anesthetic depth for intravenous agents has not been well defined. In this study, using rodents, the relationship between the constant plasma thiopental concentrations and the clinical response to multiple nociceptive stimuli were investigated characterizing the anesthetic state from light sedation to deep anesthesia and correlated to the degree of electroencephalogram (EEG) drug effect. Methods Thirty rats were instrumented with chronically implanted EEG electrodes, arterial and venous catheters. A computer-driven infusion pump was used to rapidly attain and then maintain constant, target plasma thiopental concentrations ranging from 7 to 100 micro gram/ml. Three different target plasma thiopental concentrations were achieved in each rat. Electroencephalographic effects were monitored with aperiodic waveform analysis. The following nociceptive stimuli were applied: (1) unprovoked righting reflex, (2) provoked righting reflex, (3) noise stimulus, (4) tail clamping with an alligator clip, (5) constant tail pressure with an analgesia-meter, (6) corneal reflex, and (7) tracheal intubation. For tail clamping, tail pressure, and intubation, either purposeful extremity movement or abdominal muscle contraction response was noted to be present or absent. The clinical responses (present or absent) were modeled using logistic regression to estimate the Cp50, the plasma thiopental concentration with a 50% probability of no response. Results The following mean Cp50 values (95% confidence interval) were obtained: unprovoked righting reflex, 15.9 (15.1-16.6) micro gram/ml; provoked righting reflex, 21.4 (20.2-22.7) micro gram/ml; noise stimuli, 31.3 (29.7-33.0) micro gram/ml; tail clamp and limb movement, 38.3 (36.1-40.4) micro gram/ml, tail pressure and limb movement, 39.2 (37.1-41.3) micro gram/ml; tail pressure and abdominal muscle contraction, 52.5 (50.0-55) micro gram/ml; tail clamping and abdominal muscle contraction, 56.1 (50.0-56.2) micro gram/ml; corneal reflex, 60.0 (56.6-63.4) micro gram/ml; and limb movement or muscle abdominal contraction response to intubation, 67.7 (59.2-76.1) micro gram/ml. At an EEG-effect of 9.1 and 2.2 waves/s, there was a 50% chance of limb movement response to tail clamping and tracheal intubation, respectively. There was a poor relationship between the plasma thiopental concentration and the percent increase of either heart rate or mean arterial blood pressure after applying either tail pressure or tail clamp stimuli. Conclusions A range of nociceptive stimuli and their observed clinical responses can be used to quantitate thiopental anesthetic depth, ranging from light sedation to deep anesthesia (isoelectric EEG and unresponsive to intubation) in the rodent. Clinical response can be mapped to surrogate EEG measures.
Identification of Common Binding Sites for Calmodulin and Inositol 1,4,5-Trisphosphate Receptors on the Carboxyl Termini of Trp Channels*

Homologues of Drosophila Trp (transient receptor potential) form plasma membrane channels that mediate Ca2+ entry following the activation of phospholipase C by cell surface receptors. Among the seven Trp homologous found in mammals, Trp3 has been shown to interact with and respond to IP3 receptors (IP3Rs) for activation. Here we show that Trp4 and other Trp proteins also interact with IP3Rs. The IP3R-binding domain also interacts with calmodulin (CaM) in a Ca2+-dependent manner with affinities ranging from 10 nm for Trp2 to 290 nm for Trp6. In addition, other binding sites for CaM and IP3Rs are present in the α but not the β isoform of Trp4. In the presence of Ca2+, the Trp-IP3R interaction is inhibited by CaM. However, a synthetic peptide representing a Trp-binding domain of IP3Rs inhibited the binding of CaM to Trp3, -6, and -7 more effectively than that to Trp1, -2, -4, and -5. In inside-out membrane patches, Trp4 is activated strongly by calmidazolium, an antagonist of CaM, and a high (50 μm) but not a low (5 μm) concentration of the Trp-binding peptide of the IP3R. Our data support the view that both CaM and IP3Rs play important roles in controlling the gating of Trp-based channels. However, the sensitivity and responses to CaM and IP3Rs differ for each Trp.
A Novel Sulfonylurea Receptor Forms with BIR (Kir6.2) a Smooth Muscle Type ATP-sensitive K+ Channel*

We have isolated a cDNA encoding a novel isoform of the sulfonylurea receptor from a mouse heart cDNA library. Coexpression of this isoform and BIR (Kir6.2) in a mammalian cell line elicited ATP-sensitive K+ (KATP) channel currents. The channel was effectively activated by both diazoxide and pinacidil, which is the feature of smooth muscle KATP channels. Sequence analysis indicated that this clone is a variant of cardiac type sulfonylurea receptor (SUR2). The 42 amino acid residues located in the carboxyl-terminal end of this novel sulfonylurea receptor is, however, divergent from that of SUR2 but highly homologous to that of the pancreatic one (SUR1). Therefore, this short part of the carboxyl terminus may be important for diazoxide activation of KATP channels. The reverse transcription-polymerase chain reaction analysis showed that mRNA of this clone was ubiquitously expressed in diverse tissues, including brain, heart, liver, urinary bladder, and skeletal muscle. These results suggest that this novel isoform of sulfonylurea receptor is a subunit reconstituting the smooth muscle KATP channel.
How effective is orthotic treatment in patients with recurrent diabetic foot ulcers?

BACKGROUND We assessed the efficacy of customized foot orthotic therapy by comparing reulceration rates, minor amputation rates, and work and daily living activities before and after therapy. Peak plantar pressures and peak plantar impulses were compared with the patients not wearing and wearing their prescribed footwear. METHODS One hundred seventeen patients with diabetes were prescribed therapeutic insoles and footwear based on the results of a detailed biomechanical study and were followed for 2 years. All of the patients had a history of foot ulcers, but none had undergone previous orthotic therapy. RESULTS Before treatment, the reulceration rate was 79% and the amputation rate was 54%. Two years after the start of orthotic therapy, the reulceration rate was 15% and the amputation rate was 6%. Orthotic therapy reduced peak plantar pressures in patients with reulcerations and in those without (P < .05), although a significant decrease in peak plantar impulses was achieved only in patients not experiencing reulceration. Sick leave was reduced from 100% to 26%. CONCLUSIONS Personalized orthotic therapy targeted at reducing plantar pressures by off-loading protects high-risk patients against reulceration. Treatment reduced the reulceration rate and peak plantar pressures, leading to patients' return to work or other activities.
Impact of Gender and Sex Work on Sexual and Injecting Risk Behaviors and Their Association With HIV Positivity Among Injecting Drug Users in an HIV Epidemic in Togliatti City, Russian Federation

Objectives: Togliatti City is witness to a large epidemic of human immunodeficiency virus (HIV) associated with injecting drug use (IDU). Goal: This study sought to examine whether risk behaviors and risk factors associated with HIV differed across a sample IDUs by gender and sex work. Study: A sample of IDU (n = 423) comprising female sex workers (SWs) (n = 66), non–sex workers (nonSWs) (n = 89) and men (n = 268) were recruited by field workers in community settings. Behavioral and HIV prevalence data were collected. Results: HIV prevalence did not differ across the groups (∼56%), but gender adversely affected some risk factors. A comparison of risk behaviors indicated that SWs were more likely to engage in risky injecting behaviors than either men or nonSWs. They were also more likely to report a history of sexually transmitted infections. Conclusions: IDUs involved in sex work and IDU nonSWs require specific and targeted interventions to facilitate safer injecting and sexual behaviors.
Grouping Association Rules Using Lift

Association rule mining is a well established and popular data mining method for finding local dependencies between items in large transaction databases. However, a practical drawback of mining and efficiently using association rules is that the set of rules returned by the mining algorithm is typically too large to be directly used. Clustering association rules into a small number of meaningful groups would be valuable for experts who need to manually inspect the rules, for visualization and as the input for other applications. Interestingly, clustering is not widely used as a standard method to summarize large sets of associations. In fact it performs poorly due to high dimensionality, the inherent extreme data sparseness and the dominant frequent itemset structure reflected in sets of association rules. In this paper, we review association rule clustering and their shortcomings. We then propose a simple approach based on grouping columns in a lift matrix and give an example to illustrate its usefulness.