Miracle-dz/UniFAR-FTUs huggingface dataset:
Translation Stress Positively Regulates MscL-Dependent Excretion of Cytoplasmic Proteins

ABSTRACT The apparent mislocalization or excretion of cytoplasmic proteins is a commonly observed phenomenon in both bacteria and eukaryotes. However, reports on the mechanistic basis and the cellular function of this so-called “nonclassical protein secretion” are limited. Here we report that protein overexpression in recombinant cells and antibiotic-induced translation stress in wild-type Escherichia coli cells both lead to excretion of cytoplasmic protein (ECP). Condition-specific metabolomic and proteomic analyses, combined with genetic knockouts, indicate a role for both the large mechanosensitive channel (MscL) and the alternative ribosome rescue factor A (ArfA) in ECP. Collectively, the findings indicate that MscL-dependent protein excretion is positively regulated in response to both osmotic stress and arfA-mediated translational stress. IMPORTANCE Protein translocation is an essential feature of cellular organisms. Bacteria, like all single-cell organisms, interact with their environment by translocation of proteins across their cell membranes via dedicated secretion pathways. Proteins destined for secretion are directed toward the secretion pathways by the presence of specific signal peptides. This study demonstrates that under conditions of both osmotic stress and translation stress, E. coli cells undergo an excretion phenomenon whereby signal peptide-less proteins are translocated across both the inner and outer cell membranes into the extracellular environment. Confirming the presence of alternative translocation/excretion pathways and understanding their function and regulation are thus important for fundamental microbiology and biotechnology. IMPORTANCE Protein translocation is an essential feature of cellular organisms. Bacteria, like all single-cell organisms, interact with their environment by translocation of proteins across their cell membranes via dedicated secretion pathways. Proteins destined for secretion are directed toward the secretion pathways by the presence of specific signal peptides. This study demonstrates that under conditions of both osmotic stress and translation stress, E. coli cells undergo an excretion phenomenon whereby signal peptide-less proteins are translocated across both the inner and outer cell membranes into the extracellular environment. Confirming the presence of alternative translocation/excretion pathways and understanding their function and regulation are thus important for fundamental microbiology and biotechnology.
Comparing accuracy of cosine-based similarity and correlation-based similarity algorithms in tourism recommender systems

Recommender system has a long history as a successful application in artificial intelligence. A growth in the number of products, which has been offered by different e-commerce platforms, leads to a technology which can help customers to choose and buy products. Collaborative filtering or recommender systems use a database about user preferences to predict additional topics or products a new user might like. This paper describes some algorithms designed for this task including cosine-based similarity algorithm and correlation-based similarity algorithm. The predictive accuracy of various methods in tourism recommender domains is compared. On the other hand, we have designed and implemented a recommender system in e-tourism in order to compare performance of these algorithms. Finally, we conclude that correlation based similarity algorithm acts better than Cosine based similarity algorithm.
Sensitivity and Specificity of Various Tests for the Diagnosis of Helicobacter pylori in Egyptian Children

OBJECTIVES. Many noninvasive methods (using breath, blood, and stool samples) are available to diagnose Helicobacter pylori. However, because the noninvasive tests are proxy measures of the infection, they need validation before use. Factors that may affect test validity include patient age, gender, and geographic location. Because no data were available on the validation of noninvasive tests for the diagnosis of H pylori among children in the Middle East, this study was performed. METHODS. Children between 2 and 17 years of age evaluated at the Cairo University School of Medicine pediatric gastroenterology clinic who were already scheduled for upper endoscopy were eligible for enrollment in the study. At the time of endoscopy, 3 biopsies were collected and used for rapid urease, histology, and culture, respectively. All children also donated a sample of stool and blood and had a urea breath test performed. Stool and serum samples were tested for the presence of H pylori by using commercially available enzyme-linked immunosorbent assay–based technology. The sensitivity, specificity, and positive and negative predictive values were calculated for each noninvasive test used in the study. Receiver operating curves also were charted to determine optimal cut points for the various tests when used in the current study cohort. RESULTS. One hundred eight children were enrolled in the study, with 52 children being under 6 years of age. The urea breath test and HpStar (DakoCytomation, Norden, Denmark) stool enzyme-linked immunosorbent assay kit had the highest sensitivity and specificity (sensitivity and specificity: 98 and 89 [urea breath test] and 94 and 81 [HpStar], respectively), whereas the serologic kit had an unacceptably low sensitivity (50%). The sensitivity of neither the urea breath test nor the HpStar tests was affected by subject age, but specificity of the HpStar test, although still high, was significantly lower among children under 6 years. Receiver operating curves found optimal cut points of the urea breath test at 6.2 δ over baseline and of the HpStar at 0.25 enzyme-linked immunosorbent assay units. CONCLUSION. The urea breath test and HpSTAR stool antigen kit are reliable tests for the noninvasive diagnosis of H pylori among children living in the Middle East.
Associations of psoriatic arthritis and cardiovascular conditions in a large population.

PURPOSE We studied the hypothesis that possibly via shared inflammatory mechanisms, psoriatic arthritis (PsA) is associated with increased prevalence of cardiovascular (CV) conditions. METHODS Among a multiethnic population of 76,465 men and women with known demographics, we studied all persons (n = 99) with confirmed outpatient diagnoses of PsA. Associations of PsA with CV diagnoses were studied in the entire population by logistic regression with six covariates. With two matched control study subjects for each study subject with PsA, selected risk traits for CV conditions at time of PsA diagnosis were compared with findings on t-tests. RESULTS Study subjects with PsA did not exhibit more atherothrombotic disease (coronary and cerebrovascular) or diabetes mellitus but had increased prevalence of systemic hypertension and heart failure compared with study control subjects. In the case-control analysis, study subjects with PsA had a lower mean blood cholesterol, a higher mean body mass index, and a higher mean blood pressure compared with study control subjects; mean blood glucose was similar in both groups. CONCLUSIONS In this analysis the associations of PsA with CV risk factors and CV conditions are mixed. Except for increased systemic hypertension, it is unclear whether PsA is related to higher prevalence of CV disease.
Human Papillomavirus, p16, and Epidermal Growth Factor Receptor Biomarkers and CT Perfusion Values in Head and Neck Squamous Cell Carcinoma

BACKGROUND AND PURPOSE: Head and neck squamous cell carcinoma tumors positive for laboratory biomarkers hrHPV and p16 and negative for EGFR often respond better to nonsurgical organ-preservation therapy than hrHPV-negative, p16-negative, and EGFR overexpressing tumors. CTP has been shown to distinguish which locally advanced head and neck squamous cell carcinomas will respond to induction chemotherapy or chemoradiation. Our purpose was to determine whether a relationship exists between CTP measures and the expression of these laboratory biomarkers, because both appear to separate head and neck squamous cell carcinoma tumors into similar groups. MATERIALS AND METHODS: We conducted an institutional review board–approved, Health Insurance Portability and Accountability Act–compliant retrospective review of head and neck CTP in 25 patients with locally advanced head and neck squamous cell carcinoma who had signed informed consent. Eight women and 17 men, 41–80 years of age, constituted a pretreatment group of 18 patients and a palliative group of 7 patients. Tumor biopsy samples were analyzed for overexpression of hrHPV, p16, and EGFR. The hrHPV, p16, and EGFR status of the tumors was correlated with CTP parameters (MTT, BV, BF, CP) by using the Wilcoxon evaluation and Fischer exact test. RESULTS: There were significantly lower CP values in pretreatment tumors overexpressing EGFR (P = .04). CP values ≤17.23 were significantly correlated with EGFR overexpression (P = .015). A trend toward higher CP values was present in hrHPV-positive and p16-overexpressing pretreatment tumors (P = .14). CONCLUSIONS: A significant correlation exists between CTP measures and EGFR overexpression in head and neck squamous cell carcinomas, suggesting an association between certain imaging findings and molecular biomarkers. These results may be related to a tumor cell survival mechanism linking perfusion and biomarker expression.
Dietary Nutrients and Insulin Resistance in Urban Asian Indian Adolescents and Young Adults

Background: Asian Indians have a high prevalence of insulin resistance that may underlie their higher tendency to develop type 2 diabetes mellitus and early-onset atherosclerosis. Objective: To investigate the relationship between dietary nutrients and insulin resistance in Asian Indian adolescents and young adults. Design: Dietary nutrient intake values (24-hour dietary recall and monthly consumption data) and fasting serum insulin levels were studied in 352 (311 males and 41 females) healthy adolescents and young adults (mean age 18.0 ± 2.3 years; range 14–25 years). Bivariate and multivariate logistic regression analyses were performed with hyperinsulinemia as the outcome variable and various dietary nutrients and anthropometric variables as covariates. Results: Mean fasting serum insulin levels were 107.4 ± 35.0 pmol/l (36.5–230.4 pmol/l). The intake of polyunsaturated fatty acids (PUFAs) was higher, saturated fat and the ω–6 to ω–3 PUFA ratio were in the upper limit, and ω–3 PUFAs (% caloric intake, En) were lower than the recommended dietary allowance for Asian Indians. The PUFAs (% En), BMI, percent body fat and waist circumference were significantly higher in the hyperinsulinemic group compared with the normoinsulinemic group (p = 0.021, 0.0021, 0.0006, and 0.0041, respectively). Multiple regression analysis showed that the lowest tertile of ω–6 (<3% En) PUFA intake [adjusted OR (95% CI) = 0.3 (0.1–0.7)] and BMI [adjusted OR (95% CI) = 2.9 (1.4–6.0)] were the significant independent predictors of fasting hyperinsulinemia. Conclusion: For prevention and amelioration of insulin resistance in Asian Indian adolescents and young adults, it is prudent to have normal BMI and low intake of ω–6 PUFAs.
PATHOLOGIC FINDINGS IN PATHOLOGIC MYOPIA

A retrospective study was conducted of 308 eyes with pathologic myopia obtained from 202 patients (23 surgical eyes; 285 post mortem eyes) over a 67-year period. Histopathologic findings and percentage of eyes affected, in decreasing order of frequency, were myopic configuration of the optic nerve head, 37.7%; posterior staphyloma, 35.4%; degenerative changes of the vitreous, 35.1%; cobblestone degeneration, 14.3%; myopic degeneration of the retina, 11.4%; retinal detachment, 11.4%; retinal pits, holes, or tears, 8.1%; subretinal neovascularization, 5.2%; lattice degeneration, 4.9%; Fuchs spot, 3.2%; and lacquer cracks, 0.6%. The reasons for enucleation in the surgically obtained eyes included, in decreasing order of frequency: degeneration after retinal detachment; secondary glaucoma; endophthalmitis; postsurgical epithelial ingrowth; expulsive hemorrhage; degeneration after cataract extraction; and presumed intraocular tumor. Clinicopathologic correlations are discussed.
Melatonin prevents hypertension and increased asymmetric dimethylarginine in young spontaneous hypertensive rats

Abstract: Nitric oxide (NO) deficiency is associated with development of hypertension. We examined whether melatonin protects against the blood pressure increase is because of the restoration of the NO pathway. Spontaneous hypertensive rats (SHR) and control normotensive Wistar Kyoto (WKY) rats aged 4 weeks were assigned to four groups (N = 6 for each group): untreated SHR and WKY, melatonin‐treated SHR and WKY. Melatonin‐treated rats received 0.01% melatonin in drinking water for 8 wks. All rats were sacrificed at 12 wk of age. SHR had higher blood pressure than WKY, which melatonin prevented. Plasma asymmetric dimethylarginine (ADMA) levels were elevated in SHR, combined with a reduction in plasma L‐arginine to ADMA ratio (AAR). In the kidney, L‐arginine, ADMA, and AAR were not different between SHR and WKY rats, whereas L‐citrulline level was increased in SHR. Melatonin decreased plasma ADMA level and restored plasma AAR. Renal dimethylarginine dimethylaminohydrolase (DDAH, ADMA‐metabolizing enzyme) activity was lower in SHR than WKY rats, which melatonin therapy prevented. Also, melatonin elevated both L‐arginine and ADMA but reduced L‐citrulline level in the kidney in SHR, which was associated with the prevention of reduced renal argininosuccinate lyase (ASL) expression in SHR. Moreover, melatonin reduced the degree of oxidative damaged DNA product, 8‐ hydroxydeoxyguanosine (8‐OHdG) immunostaining in SHR. The observed antihypertensive effects of melatonin in young SHR are because of the restoration of the NO pathway by reduction of plasma ADMA, restoration of plasma AAR, preservation of renal L‐Arg availability, and attenuation of oxidative stress.
Requirements for in vivo IFN-γ induction by live microfilariae of the parasitic nematode, Brugia malayi

Lymphatic filariasis caused by the parasitic nematode, Brugia malayi, is a chronic human disease immunologically characterized by stimulation of Th2 cells and reduced antigen-specific T cell responses. Single stage intra-peritoneal infections with infective larvae (L3) or adult nematodes induce Th2 cells, while the microfilarial stage (Mf) stimulates IFN-γ and Mf-specific IgG1, IgG2a, IgG2b, IgG3 and IgM, but not IgE. To investigate whether IFN-γ is elicited by live Mf in their natural site of infection, mice were infected intravenously. Intravenous infection had a striking effect on the response to Mf and high levels of IgE were induced even in the presence of IFN-γ. Indeed IgE levels to Mf increased markedly with the number of immunizations, higher doses of Mf and prolonged exposure to Mf suggesting that under conditions of chronic antigen exposure, typical of human disease, Mf will stimulate high levels of IgE. The ability of Mf-induced IFN-γ to modulate or regulate a pre-existing Th2 response, was investigated by infecting mice initially with adult male worms to induce a Th2 response, followed 14 days later by infection with Mf. Although Mf stimulated IFN-γ in the presence of male adults, the antibody isotypes elicited did not reflect IFN-γ induction and IgG1and IgE dominated the response. Although it cannot be discounted that IFN-γ induction by Mf may act locally as an inflammatory mediator or modulator of Th2 cells, these data suggest that Mf-stimulated IFN-γ does not have a profound effect overall on progression of the Th2-dominated immune response to filarial infection.
Predictive Policing: Review of Benefits and Drawbacks

ABSTRACT This literature review illuminates the conceptualization of predictive policing, and also its potential and realized benefits and drawbacks. The review shows a discrepancy between the considerable attention for potential benefits and drawbacks of predictive policing in the literature, and the empirical evidence that is available. The empirical evidence provides little support for the claimed benefits of predictive policing. Whereas some empirical studies conclude that predictive policing strategies lead to a decrease in crime, others find no effect. At the same time, there is no empirical evidence at all for the claimed drawbacks. We conclude that the current thrust of predictive policing initiatives is based on convincing arguments and anecdotal evidence rather than on systematic empirical research. We urge the research community to do independent tests of both positive and negative expectations to generate an evidence base for predictive policing.
Histomorphometry of dominant and subordinate bovine ovarian follicles

The study was designed to quantitatively characterize the histomorphological attributes of dominant and subordinate follicles in relation to follicular wave dynamics. Heifers (n = 27) were examined daily using ultrasonography to record the growth of individual follicles from 2 days before ovulation until the day of ovariectomy to obtain growing (n = 7), early static (n = 6), late static (n = 6) and regressing (n = 5) phase anovulatory dominant follicles of Wave 1, as well as preovulatory (n = 6) and subordinate (n = 42) follicles. The wall thickness of Wave 1 dominant follicles decreased dramatically (P < 0.01) during the late‐static (60.2 ± 4.3 μm) and regressing (41.8 ± 4.3 μm) phases compared to earlier phases. Cells of the granulosa layer of the dominant follicle of Wave 1 became loose during the late‐static phase, with an increase (P < 0.001) in number of degenerating cells. Dominant follicles of Wave 1 were lined by fibroblast‐like flattened cells during the regressing phase. One day after wave emergence (i.e., before selection), the three largest follicles of the wave were histomorphologically indistinguishable. The wall of the preovulatory follicle close to the medulla of the ovary was thicker (P < 0.01) than the wall facing the ovarian surface. The wall of subordinate follicles was thinner (P < 0.01) and had a lower mitotic index (P < 0.01) than that of their dominant counterparts 3 days and 6 days after wave emergence. In summary, follicular status, ascribed by ultrasonography, was associated with quantitatively distinct histomorphological characteristics. Morphometric changes in the dominant follicle during immature, mature, and post‐mature phases were similar to, but occurred later than, those of subordinate follicle. The dominant follicles of Wave 1 entered histological atresia at the time of emergence of Wave 2. Anat Rec 258:58–70, 2000. © 2000 Wiley‐Liss, Inc.
An output synchronization control design for systems with input magnitude constraints

This paper proposes design procedures for output synchronization problems where each plant is subject to control magnitude constraints. To motivate the present control design problem, we first show that existing output synchronization control law, which modifies primal reference inputs to obtain better output synchronizations, provides adequate synchronization performance, however, it tends to be high gain controls and becomes difficult for practical implementations due to well known input saturation phenomena and resulting serious performance deterioration. To overcome the problem, we propose output synchronization and Reference Governor combined control systems design procedures. Effectiveness of the proposed methods is validated through experimental evaluations.
Cytotoxic Evaluation of Semisynthetic Ester and Amide Derivatives of Oleanolic Acid

Although a number of chemicals have been isolated from Lantana Camara, only a few have been evaluated for their biological significance. As part of our drug discovery program for cytotoxic agents from Indian medicinal plants, roots of L. camara L. were chemically investigated, which resulted in the isolation and identification of a cytotoxic agent, oleanolic acid (1b) as a major constituent. Oleanolic acid was converted into six semisynthetic ester (2-7) and seven amide (8-14) derivatives. The ester derivatives (2-7) showed 3-6 times more selective activity than 1b against the human ovarian cancer cell line (IGR-OV-1), while amide derivatives 8-14 showed 16-53 times more selective activity against the human lung cancer cell line (HOP-62). Structure activity relationship within the ester (2-7) and amide (8-14) derivatives are discussed.
The Effect of Corporate Governance on Firm Stock Volatility in Asia

ABSTRACT The authors examine whether firm corporate governance (CG) contributes to lower stock-return volatility. Using the panel data of 1,252 public listed firms in Asia across 11 countries for 15 years, the authors document international evidence that CG has a stabilizing effect on firm stock-return volatility. The authors further examine whether increasing information efficiency, reducing foreign exposure, and a lower cost of capital contribute to the stabilizing effect of firm CG on stock-return volatility. The result implies that better CG will only reduce stock-return volatility for firms that have less foreign exposure.
Botswana development vision and localisation of UN Sustainable Development Goals

This paper considers how the Botswana government could use the experiences of implementing the UN Millennium Development Goals (MDGs) to localise their successor Sustainable Development Goals (SDGs) in the country’s new development strategy Vision 2036. Despite the recentralisation of some elements of service delivery (water, education, and health) reversing the decentralisation trend, Botswana attained respectable successes in achieving MDG targets. The localisation of development goals must however go beyond simply establishing effective and efficient decentralised local government units, to implementing local economic development strategies that enable communities to take an active role in national development processes. The primary question that this discussion paper seeks to answer is: can Botswana utilise lessons learnt in implementing the MDGs to the SDGs, to foster an empowered local community? The paper highlights how community empowerment is particularly critical in Botswana; on the one hand given the current over-dependence of the economy on a limited number of extractive, finite mineral resources, and on the other because of the multi-dimensional character of poverty and high income inequality afflicting Botswanans. The call for greater decentralisation in Botswana’s Vision 2036 provides a good example for the Commonwealth as it goes beyond the SDGs’ target date of 2030. KeywordsBotswana; Millennium Development Goals; Sustainable Development Goals; Vision 2036.