Miracle-dz/UniFAR-FTUs huggingface dataset:
The effectiveness of pre- and post-zygotic barriers in avoiding hybridization between two snapdragons (Antirrhinum L.: Plantaginaceae)

Reproductive barriers play an important role in the maintenance of species boundaries. However, to date, few studies have provided a detailed analysis of reproductive isolation barriers between species or examined their importance in maintaining species identity. This is the first detailed study into pre- and post-zygotic reproductive isolation barriers in Antirrhinum, based on a mixed population with two species that rarely co-occur. The study revealed that pollinator constancy and preference and poor hybrid seed viability were the most important reproductive isolating mechanisms. Reproductive isolation was practically complete by both pre- and post-zygotic barriers. Average pre-zygotic isolation was greater than post-zygotic isolation, in accordance with the trend observed in flowering plants in which reproductive isolation is principally caused by pre-zygotic mechanisms. However, average post-zygotic isolation was also high, in contrast to what was expected among Antirrhinum spp. This case highlights the importance of quantifying the reproductive isolation barriers thoroughly to understand how and why species boundaries are maintained. © 2014 The Linnean Society of London, Botanical Journal of the Linnean Society, 2014, 176, 159–172.
Topographic and ungulate regulation of soil C turnover in a temperate grassland ecosystem

A major obstacle for predicting the effects of climate and land use changes on global soil carbon (C) stores is the very limited knowledge about the long timescale dynamics of the relatively stable fraction of soil C, which represents the bulk of soil C and the primary determinant of the long‐term C balance of terrestrial ecosystems. In this study, we examined how variable topo‐edaphic conditions and herds of native migratory ungulates influenced turnover of the stable pool (total minus active fraction) of soil C in grasslands of Yellowstone National Park (YNP). Soil C properties were determined for grasslands located inside and outside long‐term ungulate exclosures established 1958–1962 at seven variable topographic positions. Active C pool sizes, estimated with soil laboratory incubations, and soil radiocarbon measures were used to parameterize a process‐based model to determine turnover of the stable C pool at the sites. Stable C turnover ranged 37–653 and 89–869 years for 0–10 and 0–20 cm soils, respectively. Among ungrazed communities, there was a trend for stable soil C turnover to slow along topographic gradients of increasing soil moisture, soil C content, and shoot biomass from hilltop to slope‐bottom positions. This was likely a result of an increasing amount of support tissue resulting in greater concentrations of lignin and cellulose as shoot biomass increased down slope. In contrast, across the grazed landscape, stable C turnover sped up from hilltop to slope‐bottom positions, which was likely a consequence of grazer effects on plant species composition along the topographic gradient. These findings indicated that despite topography playing the primary role in controlling such important site characteristics as soil moisture, soil C content, and plant production in YNP grassland, the long‐term turnover of the stable C pool was determined by herbivores. The results demonstrate the important regulatory role of herbivores in controlling the C balance of this semiarid grassland ecosystem.
Spelunking in Sputum: Single-Cell RNA Sequencing Sheds New Insights into Cystic Fibrosis

generalizable to settings with less interconnected electronic health records (e.g., the United States). Out of the 302 included patients, none had a right-heart catheterization; premorbid central venous pressure was instead estimated from echocardiography (25%) or cardiac disease history (75%). Using MAP deficit may be more pragmatic, therefore, given its relatively similar performance characteristics and reliance on less premorbid data. Randomized controlled trials to assess the value of individualized blood pressure targets are warranted. Such personalization is not antithetical to protocolization. Rather, protocolized tailoring of vasopressor titration based on individualized targets—akin to protocolized tailoring of ventilator settings based on predicted body weight (10)—may allow us to realize the best of both worlds: standardization with a personal touch. n
Characterization of the action of selected essential oil components on gram-negative bacteria

Carvacrol, (+)-carvone, thymol, and trans-cinnamaldehyde were tested for their inhibitory activity against Escherichia coli O157:H7 and Salmonella typhimurium. In addition, their toxicity to Photobacterium leiognathi was determined, utilizing a bioluminescence assay. Their effects on the cell surface were investigated by measuring the uptake of 1-N-phenylnaphthylamine (NPN), by measuring their sensitization of bacterial suspensions toward detergents and lysozyme, and by analyzing material released from cells upon treatment by these agents. Carvacrol, thymol, and trans-cinnamaldehyde inhibited E. coli and S. typhimurium at 1-3 mM, whereas (+)-carvone was less inhibitory. trans-Cinnamaldehyde was the most inhibitory component toward P. leiognathi. Carvacrol and thymol disintegrated the outer membrane and released outer membrane-associated material from the cells to the external medium; such release by (+)-carvone or trans-cinnamaldehyde was negligible. Of the tested components, carvacrol and thymol decreased the intracellular ATP pool of E. coli and also inreased extracellular ATP, indicating disruptive action on the cytoplasmic membrane.
ISOMETRIC TENSION STUDIES ON CHICKEN PECTORALIS MAJOR MUSCLE

Broiler Pectoralis major developed and released isometric tension in phosphate buffer at varying temperatures and pH levels. The presence of 1 mM calcium or magnesium in the incubation buffer enhanced tension release as did EDTA and combinations of calcium-EDTA or magnesium-EDTA. Tension release rate varied among birds being fastest in those with the shortest time to maximum tension. Amount of tension released 2 hr post-maximum tension correlated significantly (P < 0.01) with amount released from 3 through 12 hr. 2-hr values correlated negatively (P < 0.01) with time to maximum tension but not tenderness (shear value), suggesting that amount of tension release is not indicative of tenderness differences among birds.
How to Build a Biological Machine Using Engineering Materials and Methods

We present work in 3D printing electric motors from basic materials as the key to building a self-replicating machine to colonise the Moon. First, we explore the nature of the biological realm to ascertain its essence, particularly in relation to the origin of life when the inanimate became animate. We take an expansive view of this to ascertain parallels between the biological and the manufactured worlds. Life must have emerged from the available raw material on Earth and, similarly, a self-replicating machine must exploit and leverage the available resources on the Moon. We then examine these lessons to explore the construction of a self-replicating machine using a universal constructor. It is through the universal constructor that the actuator emerges as critical. We propose that 3D printing constitutes an analogue of the biological ribosome and that 3D printing may constitute a universal construction mechanism. Following a description of our progress in 3D printing motors, we suggest that this engineering effort can inform biology, that motors are a key facet of living organisms and illustrate the importance of motors in biology viewed from the perspective of engineering (in the Feynman spirit of “what I cannot create, I cannot understand”).
State of the Art Biocompatible Gold Nanoparticles for Cancer Theragnosis

Research on cancer theragnosis with gold nanoparticles (AuNPs) has rapidly increased, as AuNPs have many useful characteristics for various biomedical applications, such as biocompatibility, tunable optical properties, enhanced permeability and retention (EPR), localized surface plasmon resonance (LSPR), photothermal properties, and surface enhanced Raman scattering (SERS). AuNPs have been widely utilized in cancer theragnosis, including phototherapy and photoimaging, owing to their enhanced solubility, stability, biofunctionality, cancer targetability, and biocompatibility. In this review, specific characteristics and recent modifications of AuNPs over the past decade are discussed, as well as their application in cancer theragnostics and future perspectives. In the future, AuNP-based cancer theragnosis is expected to facilitate the development of innovative and novel strategies for cancer therapy.
Evaluation of Risk Factors for Epilepsy in Pediatric Patients with Cerebral Palsy

Cerebral palsy (CP) is a set of etiologically diverse symptoms that change with the child’s age. It is one of the most frequent causes of motor disability in children. CP occurs at a frequency of 1.5 to 3.0 per 1000 live-born children. CP often coexists with epilepsy, which is drug-resistant in a high number of cases. The aim of the present study was to analyze the associations between preconception, prenatal, perinatal, neonatal, and infancy risk factors for epilepsy in a group of pediatric patients with CP. We retrospectively analyzed 181 children with CP (aged 4–17 years at diagnosis), hospitalized at the Department of Pediatrics and Developmental Age Neurology in Katowice in the years 2008–2016. Division into particular types of CP was based on Ingram’s classification. Data were analyzed using STATISTICA 13.0 (STATSOFT; Statistica, Tulsa, OK, USA). Epilepsy was diagnosed in 102 children (56.35%), of whom 44 (43%) had drug-resistant epilepsy; only in 15 cases (14.71%) was epilepsy susceptible to treatment. The incidence of epilepsy varied between the types of CP. It occurred significantly more often in children with tetraplegia (75%), ataxic form (83%), and mixed form (80%) in comparison to diplegia (32%) and hemiplegia (38%). Maternal hypertension was found to be a risk factor for epilepsy in CP patients (OR = 12.46, p < 0.001) as well as for drug-resistant epilepsy (the odds ratio (OR) = 9.86, p = 0.040). Delivery by cesarean section increased the risk of epilepsy in the CP patients over two-fold (OR = 2.17, p = 0.012). We observed also that neonatal convulsions significantly increased the risk for epilepsy (OR = 3.04, p = 0.011) as well as drug-resistant epilepsy (OR = 4.02, p = 0.002). In conclusion, maternal hypertension, neonatal convulsions, and delivery by cesarean section were the most important factors increasing the risk of epilepsy as well as drug-resistant epilepsy in the analyzed group of patients with CP.
Association between neighborhood aesthetics and childhood obesity

The lack of neighbourhood aesthetics (e.g. public art and well‐maintained properties) may reduce walkability in the neighbourhood and increase the risk of childhood obesity. In this study, a literature search was conducted in the Cochrane Library, PubMed and Web of Science for articles published before January 1, 2019 to analyse the associations between neighbourhood aesthetics and weight‐related behaviours and outcomes among children and adolescents aged <18. One cohort study and 24 cross‐sectional studies, conducted in 10 countries with a median sample size of 1124 were identified. Neighbourhood aesthetics was more commonly assessed by self‐reported or parent‐reported perceptions than objective measurements. Eighteen of the 25 included studies analysed physical activity (PA) as the outcome of interests, eight studies analysed active transport to school (ATS), and eight studies analysed weight status, including body mass index and overweight/obesity status. About two‐thirds of studies reported non‐significant associations when using PA and weight status as outcomes, and half of studies showed that neighbourhood aesthetics is associated with increased use of ATS. The rest of the studies reported mixed findings with slightly more studies showing neighbourhood aesthetics may promote PA or reduce weight. Better designed studies are necessary to achieve a robust understanding of this epidemiological relationship in the future.
Epigenetic Clock: DNA Methylation in Aging

Aging, which is accompanied by decreased organ function and increased disease incidence, limits human lifespan and has attracted investigators for thousands of years. In recent decades, with the rapid development of biology, scientists have shown that epigenetic modifications, especially DNA methylation, are key regulators involved in this process. Regular fluctuations in global DNA methylation levels have been shown to accurately estimate biological age and disease prognosis. In this review, we discuss recent findings regarding the relationship between variations in DNA methylation level patterns and aging. In addition, we introduce the known mechanisms by which DNA methylation regulators affect aging and related diseases. As more studies uncover the mechanisms by which DNA methylation regulates aging, antiaging interventions and treatments for related diseases may be developed that enable human life extension.
Molecular Characterization and Pathogenicity of Chicken Parvovirus (ChPV) in Specific Pathogen-Free Chicks Infected Experimentally

Chicken parvovirus (ChPV) is an agent frequently associated with runting stunting syndrome (RSS). This syndrome has been reported in association with ChPV in many countries, including Brazil; however, studies characterizing the virus on a molecular level are scarce, and ChPV pathogenicity in day-old chicks remains unclear. The aim of the present work was to establish the molecular characteristics of ChPV, determine the pathogenicity of ChPV in SPF chicks and detect and quantify ChPV by qPCR in several tissues and chicks of different ages. The experimental challenge was performed at one day of age, and daily and weekly observations were performed and five birds from each experimental group (mock and infected birds) were euthanized to perform the different analysis. ChPV genome copies were detected and quantified by qPCR in gut, spleen, thymus, kidney, pancreas, proventriculus and bursa. Clinically, the infected group presented with diarrhea 24 h post-infection, which persisted until 42 days of age. The small intestine was distended, and its contents were aqueous and foamy. Enteritis and dilated crypts with cyst shapes were observed in intestinal segments. Acute pancreatitis associated with lymphocytic nodules, infiltrating lymphocytes and plasma cells between the pancreatic acinus was observed. Koch’s postulate was demonstrated and the genetic characterization of the VP1 gene showed that the Brazilian ChPV isolate belongs to the ChPV II group.
Patterns in vendace recruitment in Lake Pyhäjärvi, south‐west Finland

A vendace Coregonus albula population has long formed the basis of a flourishing fishery in Lake Pyhajarvi, south-west Finland. Between 1971 and 1990, it exhibited variations in year-class strength generally characterized by a 2-year cycle of alternating strong and weak year classes, which implied density-dependent regulation probably due to asymmetrical food competition between age groups. The abundance of piscivorous predators in the lake and the wanning of water after the hatching of larvae in spring together determined the final year-class strength of vendace. Since 1990, year-class sizes have remained very low and the 2-year cycle has been disrupted. The continuously low levels of recruitment could not be explained by an increased larval mortality, although the decline started with 2 successive years with very high mortality of this life stage which reduced the whole spawning stock to a fraction of earlier levels, and consequently led to low larval numbers such that between 1988 and 1996 larval abundance was positively correlated with the spawning stock size. The importance of the spawning stock size to recruitment could also be seen in the whole 26-year time series as a significant correlation between year-class sizes with a 2-year lag. The observed recruitment patterns of the vendace stock in Lake Pyhajarvi thus show the importance of spawning stock size, intraspecific competition, predator effects and abiotic environmental variation.
Does evolution in body patterning genes drive morphological change— or vice versa?

Summary Increased understanding of the regulation of body patterning genes in development, especially the homeotic genes, has led to the revival of ideas suggesting that ‘‘saltational’’ modes of evolution are important. However, such models are problematic on the grounds of functional continuity and population genetics, and the more dramatic scenarios rely on an overinterpretation of the taxonomic hierarchy. This article proposes an alternative model for the evolution of Hox gene expression, stressing the need for incremental functional integration. One surprising implication of the model would be that mutations in Hox genes and their regulators have virtually no primary role in driving morphological evolution. Rather, morphological change through microevolutionary adaptation comes first, with Hox expression shifting only afterwards, presumably to make the building of the new body pattern more efficient or more stable. Such a model has affinities to Waddington’s ‘‘genetic assimilation’’ but invokes discrete rather than continuous shifts in control of a particular morphology. BioEssays 21:326‐332, 1999. r 1999 John Wiley & Sons, Inc.
Impact of the COVID-19 Pandemic on Tuberculosis Control: An Overview

Throughout history, pandemics of viral infections such as HIV, Ebola and Influenza have disrupted health care systems, including the prevention and control of endemic diseases. Such disruption has resulted in an increased burden of endemic diseases in post-pandemic periods. The current coronavirus disease 2019 (COVID-19) pandemic could cause severe dysfunction in the prevention and control of tuberculosis (TB), the infectious disease that causes more deaths than any other, particularly in low- and middle-income countries where the burden of TB is high. The economic and health crisis created by the COVID-19 pandemic as well as the public health measures currently taken to stop the spread of the virus may have an impact on household TB transmission, treatment and diagnostic services, and TB prevention and control programs. Here, we provide an overview of the potential impact of COVID-19 on TB programs and disease burden, as well as possible strategies that could help to mitigate the impact.
Expression of Recombinant 3-Beta Hydroxysteroid Dehydrogenase Protein in E. coli

beta hydroxysteroid dehydrogenase (3BHSD) is secreted by the cortex of adrenal gland functioning in stress conditions. The gene encoding the 3-beta hydroxysteroid dehydrogenase protein was PCR amplified from a λgt11 cDNA library using specific primers. The amplified PCR product was then cloned into pGEX-4T-1 expression vector under Ptac pro- moter and the expression of the enzyme was exam- ined in E. coli (BL21). Upon optimization of the expression condition, the enzyme was produced as a glutathione S-transferase (GST) fusion protein, which was purified by affinity chromatography using glu- tathione sepharose column. The GST part was then removed by selective proteolytic digestion with thrombin. The purified recombinant enzyme could be used in construction of diagnostic kits for screening the patients with premature ovarian failure (POF) for the presence of autoantibodies against 3BHSD as an important molecular target.
First Clinical Study with AP30663 ‐ a KCa2 Channel Inhibitor in Development for Conversion of Atrial Fibrillation

Pharmacological cardioversion of atrial fibrillation (AF) is frequently inefficacious. AP30663, a small conductance Ca2+ activated K+ (KCa2) channel blocker, prolonged the atrial effective refractory period in preclinical studies and subsequently converted AF into normal sinus rhythm. This first‐in‐human study evaluated the safety and tolerability, and pharmacokinetic (PK) and pharmacodynamic (PD) effects were explored. Forty‐seven healthy male volunteers (23.7 ± 3.0 years) received AP30663 intravenously in ascending doses. Due to infusion site reactions, changes to the formulation and administration were implemented in the latter 24 volunteers. Extractions from a 24‐hour continuous electrocardiogram were used to evaluate the PD effect of AP30663. Data were analyzed with a repeated measure analysis of covariance, noncompartmental analysis, and concentration‐effect analysis. In total, 33 of 34 adverse events considered related to AP30663 exposure were related to the infusion site, mild in severity, and temporary in nature, although full recovery took up to 110 days. After formulation and administration changes, the local infusion site reaction remained, but the median duration was shorter despite higher dose levels. AP30663 displayed a less than dose proportional increase in peak plasma concentration (Cmax) and a terminal half‐life of around 5 hours. In healthy volunteers, no effect of AP30663 was observed on electrocardiographic parameters, other than a concentration‐dependent effect on the corrected QT Fridericia’s formula interval (+18.8 ± 4.3 ms for the highest dose level compared with time matched placebo). In conclusion, administration of AP30663, a novel KCa2 channel inhibitor, was safe and well‐tolerated systemically in humans, supporting further development in patients with AF undergoing cardioversion.
High-Efficient Video Transmission for HDTV Broadcasting

Before broadcasting a video signal, redundant data should be removed from the transmitted video signal. This redundancy operation can be performed using many video coding standards such as H.264/Advanced Video Coding (AVC) and H.265/High-Efficient Video Coding (HEVC) standards. Although both standards produce a great video resolution, too much data are considered to be still redundant. The most exhaustive process in video encoding process is the Motion Estimation (ME) process. The more the resolution of the transmitted video signal, the more the video data to be fetched from the main memory. This will increase the required memory access time for performing the Motion Estimation process. In This chapter, a smart ME coprocessor architecture, which greatly reduces the memory access time, is presented. Data reuse algorithm is used to minimize the memory access time. The discussed coprocessor effectively reuses the data of the search area to minimize the overall memory access time (I/O memory bandwidth) while fully using all resources and hardware. This would speed up the video broadcasting process. For a search range of 32 × 32 and block size of 16 × 16, the architecture can perform Motion Estimation for 30 fps of HDTV video and easily outperforms many fast full-search architectures.