Miracle-dz/UniFAR-FTUs huggingface dataset:
Mitogen-Induced p53 Downregulation Precedes Vascular Smooth Muscle Cell Migration From Healthy Tunica Media and Proliferation

Abstract—The tumor suppressor protein p53 plays an important role in the cell-cycle G1 and G2 checkpoints. In response to DNA damage, p53 can induce the transcription of p21, which inhibits the activation of various G1 cyclin/cyclin-dependent kinase complexes. It is not known whether p53 plays a role in the initial migration of vascular smooth muscle cells from the arterial tunica media (mVSMCs). In this study, we have investigated whether mVSMC migration from healthy tunica media of young pigs and proliferation are regulated by p53. After 6 hours of incubation in mitogen-rich medium, explanted porcine tunica media tissue showed complete downregulation of p53 protein and p53 mRNA. The blockage of gene activity was not due to DNA methylation at the 5′ control region of the gene. The mVSMC outgrowth did not show p53 expression. Mitogen-depletion of cultured p53−/mVSMCs did not restore p53 expression. Incubation of explanted porcine tunica media tissue in mitogen-deprived medium increased p53 protein content.
Performance analysis of Strain sensor based on Fiber Bragg Grating

A fiber Bragg grating can be worked as reflector, it is constructed in a short section of optical fiber that reflects light of specific wavelength and allow to pass remaining wavelengths. This is possible by creating a periodic change in refractive index within the fiber core. The change in the modal index of fiber caused due to strain results in a Bragg wavelength. This work describes performance of Fiber Bragg Gratings as a strain sensor at 1550nm wavelength is considered for simulation, corresponding dynamic strain and wavelength shifts are analysed. This performance analysis is useful especially for intrusion detection system where environments are harsh. This sensor is compatible with data communication system and can be used for remote sensing.
Role of imaging in deep vein thrombosis: a review article

Article type: Review article Background and Aim: Venous Thrombo Embolism (VTE) refers to the formation of clots in blood vessels. The current study aimed to investigate deep vein thrombosis (DVT) by imaging modalities. Materials and Methods: In this review study, national databases, including Magiran, SID, IranMedex, as well as international ones, namely databases including PubMed, Google scholar, Scopus, and ISI, were searched for related books and articles. The keywords included thrombosis, deep vein thrombosis, imaging, and thrombosis detection. Results: In patients with suspected primary or recurrent deep vein thrombosis, CT venography (CTV) and Magnetic Resonance Venography (MRV) are rarely used on suspicion of iliac vein thrombosis or inferior vena cava (IVC) thrombosis in ultrasound. These examinations have relatively poor reliability, cause adverse side effects of radiation and contrast materials, and are undoubtedly more costly. Conclusion: As evidenced by the obtained results, different methods are available for the diagnosis of deep vein thrombosis. Moreover, it was revealed that ultrasound imaging is the most reasonable method for initial examination due to its sensitivity, specificity, costs, and adverse effects. Article History: Received: 19 November 2019 Revised: 19 January 2020 Accepted: 19 January 2020
Purification and identification immunomodulatory peptide from rice protein hydrolysates

ABSTRACT To prepare immunomodulation peptides from rice protein hydrolysates (RPHs), trypsin was employed for enzymatic hydrolysis, and the immunomodulating activities of RPHs were studied using mouse peritoneal macrophage proliferation assay. The peptide fractions with the highest activity were further purified using macroporous adsorption resin, strong cation-exchange chromatography, gel filtration chromatography and reversed-phase high-performance liquid chromatography. The peptide sequence was identified as Tyr-Gly-Ile-Tyr-Pro-Arg (YGIYPR) by high-performance liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometer. Evaluating its immunomodulatory activity showed that YGIYPR enhanced the proliferation of macrophage RAW 264.7 cells in the range of 12.5–100 μg/mL, and even a minimum dose achieved good proliferation. The results demonstrated that the RPHs prepared by trypsin could serve as a source of immunomodulating peptides and the immunomodulatory peptide YGIYPR is a potential natural ingredient for manufacture of functional foods or health care products.
MODEL CALCULATIONS OF THE UV - EXCITED MOLECULAR HYDROGEN IN INTERSTELLAR CLOUDS

We have calculated 2448 interstellar cloud models to investigate the formation and destruction of high rotational level according to the combinations of five physical conditions: the input UV intensity, the column density, cloud temperature, total density, and the formation rate efficiency. The models include the populations of all the accessible states of with the rotational quantum number J is in a steady state governed primarily by the rate of formation on the grain surfaces and the rates of destruction by spontaneous fluorescent dissociation following absorption in the Lyman and Werner band systems. The high rotational levels J
PREFERENSI PELETAKAN TELUR DAN PENGHAMBATAN PERKEMBANGAN PRADEWASA NYAMUK Aedes aegypti L. DI BERBAGAI MEDIA AIR

Water media influences the effectiveness of the use of ovitrap media for Ae. aegypti. Water media which is prefered by the mosquito as well as inhibition the premature growth have huge potency in controlling Ae. aegypti. The objective of this study were to investigate the preference of oviposition of Ae. Aegypti and to determine the premature growth of Ae. aegypti in water media containing a combination of organic matter and different chlorine concentrations, also to examine the interaction between organic matter and chlorine to the preferences of oviposition and the premature growth of Ae. Aegypti. The method of this study is done by experiment by applying Completely Randomize Design (RAL). This study used two types of water media combination. The media combinations contained organic materials and chlorine with seven different treatments. They were well water (control), water added with soil and hay-soaked water added with chlorine by 5, 10, and 15 ppm respectively. The findings show that Ae. aegypti have the tendency in choosing hay-soaked water in lay the eggs since it has abundant content of organic matter in it. The mean amount of Ae. aegypti oviposition in the water medium combination ranged from 84 to 152.7 eggs, while the percentage of premature survival rate ranged from 59.4% to 97%. In conclusion, the study showed that the preference of oviposition is highly influenced by the organic matter content of the media. Both organic matter and chlorine do not inhibit the premature growth of Ae. aegypti. However, the interaction between organic matter and chlorine to the preferences of oviposition and the premature growth of Ae. aegypti was not found. Keywords: Aedes aegypti, combination, ovitrap, organic matter, chlorine.
A method for estimating nitrogen supply index in crop plants: case study on wheat

This paper aim was to adapt a physiological parameter which reflects plant growth and productivity for wheat plants in relation with the applied nitrogen dose. This index can be used for various plants due to the fact that can be easy calculated and gives information about the status and requirements of diverse crops. Nitrogen is the most widely used chemical fertilizer and the adjustment of the dose is really important for farmers, who are searching for an optimum cost-benefit ratio. For this study, five experimental N doses were utilized, between 0 and 200 kg/ha. Experimental field was located within Didactic Station of BUASMV Timisoara, Romania. Nitrogen supply index was determined for healthy leaves and mathematical models were developed. The optimum N amount used for this study was 119 kg/ha and the analysis of differences reflected the modifications for doses ranging between 0 and 200 kg/ha.
Visibility-Based Persistent Monitoring of Piecewise Linear Features on a Terrain Using Multiple Aerial and Ground Robots

Persistent monitoring on terrains using mobile robotic sensors requires coordinated planning. Terrain features add visibility obstacles and limited fuel capacity of aerial robots leads to range restrictions that make the problem challenging. We address the visual-monitoring problem on piecewise linear features within a terrain using multiple mobile robots for persistent operations. The planner must account for visual coverage, refueling aerial robots during the mission, and placement of refueling depots while also utilizing the available sensor diversity to minimize overall costs for the monitoring mission. Building on previous works on visibility in specific classes of polygons and fuel-constrained routing, we develop a discrete representation of the problem that allows the design and application of discrete optimization techniques to find optimal solutions. We develop a mixed-integer linear programming (MILP) formulation and discuss a branch-and-cut implementation to compute exact solutions. We also develop a construction heuristic based on the idea of competitive construction of robot paths using a step-increment strategy. We report the results from computational simulations and illustrate proof of concept using experiments on real robots. Note to Practitioners—This article is motivated by the need to perform persistent monitoring in applications, such as border patrol and perimeter surveillance. Unmanned aerial and ground robots can be used to perform these activities uninterruptedly. However, aerial robots have limited fuel capacity and need periodic refueling. Hence, the number of refueling depots and their placement within the environment also affects the monitoring task. Also, due to terrain variation, robots are subject to limited visibility. Therefore, we need to consider refueling constraints and terrain visibility aspects while planning optimal routes for the robots to perform visual monitoring. In this article, we present a general optimal routing formulation to compute exact solutions. We also present a fast heuristic for real-time applications that produce feasible solutions. The algorithms are validated in simulations. We also show a proof of concept using experiments in limited outdoor settings.
Necessary and Sufficient Conditions for Containment in Fractional-Order Multiagent Systems via Sampled Data

The containment control problem in fractional-order multiagent systems over a directed graph is studied in this article. Two distributed control protocols with or without time delays are designed by utilizing the sampled position and velocity data. Then, based on the knowledge of the matrix theory and Laplace transform, some necessary and sufficient conditions to reach containment are derived. For the protocol with time delays, similar sufficient and necessary conditions are obtained. Finally, some simulation examples are designed to verify that the theorems are correct.
Nutritional Effects of Baobab (Adansonia digitata L.) Pulp-seed Meal on the Reproductive Index of Red Sokoto Goats

Oestrus was artificially synchronized in thirty-six (36) primiparous Red Sokoto goats (RSG) done using 45 mg fluorogestone acetate vagina sponges for fourteen (14) days. Pregnant mare serum gonadotrophin (PMSG) hormone was administered immediately after sponge removal and the does were mated thereafter. Twenty four (24) gravid weighing 15.8 kg – 23.7 kg were selected and fed graded levels of baobab (Adansonia digitata. L) pulp-seed meal supplement in a completely randomized experimental design of six (6) replicates per treatment. There were significant differences (P<0.05) in the results obtained. At parturition, animals on 20% baobab fruit inclusion Original Research Article Okunlola et al.; ARRB, 28(1): 1-6, 2018; Article no.ARRB. 36965 2 level had the highest mean weight value of 28.8 kg and the does on 0% inclusion level recorded the least value of 20.5 kg. At pregnancy, animals on 30% baobab inclusion level had the highest weight gain value of 5.9 kg, followed by 10% inclusion level with a weight gain of 5.5 kg. 20% inclusion level had 5.1 kg weight gain and the least value of 4.7 kg was recorded for animals on 0% baobab fruit inclusion. Similarly, the weight of dam at weaning ranged from 17.1kg to 24.8 kg. The Birth weight of kids were 1.7, 2.2, 2.1 and 2.0 kg for 0, 10, 20 and 30% levels of baobab fruits inclusion respectively. The sex ratio of male: female recorded in the study were significantly different (P<0.05). 0, 10, 20 and 30% baobab fruit inclusion recorded 33:67 (2 males, 4 females), 50:50 (3 Males, 3 Females), 83:17 (5 Males, 1 Female) and 67:33 (4 Males, 2 Females) respectively. The kid weight at weaning and daily weight gain of the kids was significantly different (P<0.05) at all levels of baobab fruit inclusion. The percentages of kid mortality at birth and at weaning were zero in this study.
Molecular testing approaches in thyroid cancer diagnosis

Thyroid cancer (TC) is the most common endocrine malignancy worldwide, with an annual incidence of around 300,000 cases. In recent decades, the incidence of TC in many countries, including Saudi Arabia, has increased significantly. Genomics has allowed multiple mutations to be examined simultaneously across multiple genes, providing a detailed genomic profile for a given tumor. It is understood from a molecular perspective that different signaling pathways may have genetic abnormalities as the primary factors in thyroid tumorigenesis. While there is still early progress in the usage and standardization of targeted next-generation sequencing (NGS) in TC, a major ongoing study to distinguish malignant from benign thyroid nodules from fine-needle aspiration (FNA) has shown promising results that can prevent unnecessary surgery, based on NGS analyses of mutations and gene fusions associated with most TC. The differential diagnosis and malignancy risk stratification of TC require multidisciplinary skills and experience of both ultrasound and the FNA and molecular analysis. The most common TC mutations are point mutations in the BRAF and the chromosome rearrangements of RET/papillary TCs. The transition mechanisms tend to be linked to specific etiological features, which are very crucial while deciding the treatment protocol. On the other hand, FNA cytology has an intrinsic drawback. The findings of infinite cytology cannot differentiate some types of TC, such as follicular adenomas, thyroid follicular carcinomas, or papillary thyroid follicular. Nevertheless, accumulating evidence indicates that molecular diagnostic approaches can overcome these limitations. In this review, we present an updated summary, which focuses primarily on molecular alterations in the tumorigenesis and biomarker investigations of TC.
Harmonic and Interharmonic Analysis of Mixed Dense Frequency Signals

Dense frequency signal refers to the signal containing harmonic and interharmonic components that are very close to each other in frequency domain. Due to the limited frequency resolution and the existence of spectrum leakage and fence effects, it is difficult to accurately detect the harmonic and interharmonic components in dense frequency signals. The spectral interference under asynchronous sampling conditions can lead to the failure of measuring the multiple adjacent harmonics and interharmonics. Therefore, this article proposes a mixed dense signal harmonic and interharmonic analysis method. The dense frequency signal model is first established. Based on the model, the discrete Fourier transform results of the dense frequency signal are processed by local mean decomposition extension to construct that single multichannel signal as a virtual multichannel. Then, the parameters of each frequency component are obtained by means of principal component analysis and fast independent component analysis. Finally, the measurement of dense harmonic and interharmonic components is implemented. The simulation and experimental results show the high accuracy and the noise resistance ability of the proposed method in identifying and measuring the dense harmonic and interharmonic components.
THE EFFECT OF SINGLE AND COMPOSITE ADDITIVES ON CD AND PB MOBILITY, SPECIATION AND ACCUMULATION IN LATE RICE (ORYZA SATIVA L.) GROWN ON CONTAMINATED SOIL

This field trial investigated the cadmium (Cd) and lead (Pb) immobilization in contaminated paddy soil. Five stabilizing agents including lime, biochar, phosphatic fertilizer (PF) and two composites (S1, S2) were investigated (1% w/w) for metals immobilization by growing late rice as a test crop. The results revealed the improved pH and decreased metal availability in lime, S1, biochar and S2 treated plots. The Cd and Pb speciation results depicted a decrease in exchangeable Cd content to less mobile forms (29 and 30%) with addition of biochar and S1 in harvest stage samples. The exchangeable fraction of Pb was significantly reduced to 29, 29 and 36% with S1, S2 and biochar, respectively. Moreover, Cd and Pb accumulation in rice grains was lowest in composite stabilizers and biochar treatments. Our results showed a significant increase in leaf photosynthetic rate, transpiration rate, biomass and grains yield in S1 amended plot. These lessening effects are owed to the conversion of available metal contents to the least mobile forms. Our results confirmed the effectiveness of organic additives alone and in mixture with inorganics in reducing metal mobility, improving immobilization, enhancing growth and decreasing accumulation in food parts and showed a great prospect in safer food production.
Building and simulating protein machines

Glycolysis is a central metabolic pathway, present in almost all organisms, that produces energy. The pathway has been extensively investigated by biochemists. There is a significant body of structural and biochemical information about this pathway. The complete pathway is a ten step process. At each step, a specific chemical reaction is catalyzed by a specific enzyme. Fructose bisphosphate aldolase (FBA) and triosephosphate isomerase (TIM) catalyze the fourth and the fifth steps on the pathway. This thesis investigates the possible substrate transfer mechanism between FBA and TIM. FBA cleaves its substrate, the six-carbon fructose-1,6-bisphosphate (FBP), into two three-carbon products – glyceraldehydes 3-phosphate (GAP) and dihydroxy acetone phosphate (DHAP). One component of these two products, DHAP, is the substrate for TIM and the other component GAP goes directly to GAPDH, the subsequent enzyme on the pathway. TIM converts DHAP to GAP and delivers the product to GAPDH. I employ Elastic Network Models (ENM) to investigate the mechanistic and dynamic aspects of the functionality of FBA and TIM enzymes – (1) the effects of the oligomerization of these two enzymes on their functional dynamics and the coordination of the individual protein’s structural components along the functional region; and (2) the mechanistic synchrony of these two protein machines that may enable them to operate in a coordinated fashion as a conjugate machine – transferring the product from FBA as substrate to TIM. A macromolecular machine comprised of FBA and TIM will facilitate the substrate catalysis mechanism and the product flow between FBA and TIM. Such a machine could
Public acceptability of government interventions to reduce obesity: policy effectiveness, policy fairness, government trust and political ideology

Abstract Frequent consumption of sugar-sweetened beverages (SSBs) has been linked to a greater risk of obesity, type 2 diabetes mellitus, dental caries and coronary heart disease. SSBs are an ideal target for public health intervention to help control the type 2 diabetes mellitus epidemics since they have absolutely no nutritional value, and do not protect against disease. However, successful implementation of policies to reduce consumption of SSBs depends on public acceptability and support. Therefore, determining the public acceptability of government interventions to reduce sugar-sweetened beverages is critical for effectiveness as well as implementation. The aim of the present study was to conduct an empirical investigation of how different policy-related factors (namely perceived policy effectiveness, perceived policy fairness, policy trust and political ideology) link to public support. Policy options included low, medium and high intensity versions of: imposing a tax (10%, 20% and 30%); adding a warning label (with and without graphic warning label); reducing number of SSB retail outlets (schools only and all public institutions); and regulating SSB advertising (ban advertising aimed at children and ban all SSB advertising). Data were collected from a representative sample of 948 consumers (age range from 18 to 69) through an online survey, which was carried out in February 2019. Sample quotas were set for age, gender and income. The partial least squares approach of the structural equation modelling (PLS-SEM) is employed to investigate direct effects of the proposed factors on public acceptance. Importantly, the results indicate that perceived fairness and perceived effectiveness of the political interventions had a significantly positive correlation with public acceptance (β = 0.518, p-value < 0.001 and β = 0.299, p-value < 0.001, respectively). Meanwhile, those who have a greater trust in government are more likely to support (β = 0.049, p-value < 0.05). Also, public acceptance is positively associated with liberal policy strains. This is in line with social cognitive motives such as openness to new experiences and desire for equality in society. Moreover, higher trust in government is positively associated with perceived fairness as well as perceived effectiveness. These findings reflect that those who trust government believe that the government policy is fair in terms of the policy itself, how it is established as well as its specific distributive effects. Therefore, participatory policymaking that involves the public in the decision-making process can enhance citizens’ trust and overall acceptance in policy interventions with regard to SSBs.
Application of Mann-Kendal (MK) test in trend analysis of air temperature and precipitation: Case of Mačva district (Serbia)

Recent climate changes cause significant natural and socio-economic consequences. They represent one of the major environmental problems of the late 20 and early 21 centuries. Changes in temperature and precipitation play a significant role in understanding climate change issues. They include numerous extreme climatic events such as heat waves, droughts, forest fires and more. Looking at regional differences in temperature and precipitation regime change, Southern Europe, including the Republic of Serbia, occupies a special place. The Mačva district (3.272 km2) is almost four times as spacious as the Mačva area. The administrative -political division of the Mačva district is comprised of eight municipalities. The paper presents trends of two climate variables: mean annual air temperature and mean annual precipitation in the study area. In methodological sense, the main statistical procedure is the non-parametric Mann-Kendall (MK) test. Data from three available meteorological stations in the Mačva district (1949-2015) were used for the analysis. The presented results of the trends in air temperature indicate that there is a statistically significant positive trend in all-time series. In trends, precipitation amounts a statistically significant positive trend was observed in two time series, while in one there is no trend. The expressed trends, especially in the case of air temperatures, lead to the possibility of drought. Mačva district and Mačva have some agricultural importance in the Republic of Serbia. For these reasons, some adaptation to climate change is needed as arid conditions lead to fluctuations in agricultural yield.