Miracle-dz/UniFAR-FTUs huggingface dataset:
Hydrocortisone Increases the Vinblastine-Induced Chromosomal Damages in L929 Cells Investigated by the Micronucleus Assay on Cytokinesis-Blocked Binucleated Cells

Background: Stress may cause damages to DNA or/and change the ability of the cells to overcome these damages. It may also cause irregularities in the cell cycle and induce abnormal cell divisions through glucocorticoid-dependent functions. The abnormal cell divisions, in turn, lead to chromosomal mal-segregation and aneuploidy. In this study, the effects of the stress hormone, hydrocortisone (HYD), were investigated on the induced chromosomal abnormalities by vinblastine (VIN) during cell cycle in L929 cells. Methods: This work was performed in winter 2013 at Department of Biology, University of Ferdowsi, Mashhad, Iran. Cultured cells were divided into different groups including control, VINtreated, HYD treated and VIN+HYD co-treated cells. The induced chromosomal damages were investigated by micronucleus assay in cytokinesis-blocked binucleated cells. Results: Although HYD by itself did not increase the micronuclei (Mn) frequency, co-treatment of cells with VIN and HYD led to significant increase (P<0.05) in the frequency of Mn in comparison to control and VIN treated groups. Conclusion: Cells treated with stress hormone are more sensitive to damages induced by VIN. Therefore, stress may not directly result in genetic instability, it can increase the harmful effects associated with other genotoxic agents.
Spatial Distribution and Temporal Variation of the Rice Silicon Transporter Lsi11

Rice (Oryza sativa) is a typical silicon (Si) accumulator and requires a large amount of Si for high-yield production. Recently, a gene (Low silicon rice1 [Lsi1]) encoding a Si transporter was identified in rice roots. Here, we characterized Lsi1 in terms of spatial distribution and temporal variation using both physiological and molecular approaches. Results from a multicompartment transport box experiment showed that the major site for Si uptake was located at the basal zone (>10 mm from the root tip) of the roots rather than at the root tips (<10 mm from the root tip). Consistent with the Si uptake pattern, Lsi1 expression and distribution of the Lsi1 protein were found only in the basal zone of roots. In the basal zones of the seminal, crown, and lateral roots, the Lsi1 protein showed a polar localization at the distal side of both the exodermis and endodermis, where the Casparian bands are formed. This indicates that Lsi1 is required for the transport of Si through the cells of the exodermis and endodermis. Expression of Lsi1 displayed a distinct diurnal pattern. Furthermore, expression was transiently enhanced around the heading stage, which coincides with a high Si requirement during this growth stage. Expression was down-regulated by dehydration stress and abscisic acid, suggesting that expression of Lsi1 may be regulated by abscisic acid.
European commission’s fiscal forecasts in CEE countries: a thorough assessment

ABSTRACT This paper assesses European Commission’s fiscal forecasts for a sample of 10 Central and Eastern European countries between 2005 and 2015. The analysis focus on forecasts of the budget balance, revenues, expenditures and debt and pays special attention to dynamics around business cycle turning points. Results suggest that the distribution of projection errors appears to be biased towards optimism of fiscal aggregates and accuracy increases as the forecast horizon shortens. We also find evidence of “forecast smoothing”. In addition, we find that, on average, the extent of optimism seems to increase during recessions (and to a lesser extent during recoveries). Moreover, errors in forecasting fiscal variables can be explained by forecasts errors of real GDP growth and inflation.
Acute seizures after intracerebral hemorrhage

Objective: To determine whether early seizures that occur frequently after intracerebral hemorrhage (ICH) lead to increased brain edema as manifested by increased midline shift. Methods: A total of 109 patients with ischemic stroke (n = 46) and intraparenchymal hemorrhage (n = 63) prospectively underwent continuous EEG monitoring after admission. The incidence, timing, and factors associated with seizures were defined. Serial CT brain imaging was conducted at admission, 24 hours, and 48 to 72 hours after hemorrhage and assessed for hemorrhage volume and midline shift. Outcome at time of discharge was assessed using the Glasgow Outcome Scale score. Results: Electrographic seizures occurred in 18 of 63 (28%) patients with ICH, compared with 3 of 46 (6%) patients with ischemic stroke (OR = 5.7, 95% CI 1.4 to 26.5, p < 0.004) during the initial 72 hours after admission. Seizures were most often focal with secondary generalization. Seizures were more common in lobar hemorrhages but occurred in 21% of subcortical hemorrhages. Posthemorrhagic seizures were associated with neurologic worsening on the NIH Stroke Scale (14.8 vs 18.6, p < 0.05) and with an increase in midline shift (+ 2.7 mm vs −2.4 mm, p < 0.03). There was a trend toward increased poor outcome (p < 0.06) in patients with posthemorrhagic seizures. On multivariate analysis, age and initial NIH Stroke Scale score were independent predictors of outcome. Conclusion: Seizures occur commonly after ICH and may be nonconvulsive. Seizures are independently associated with increased midline shift after intraparenchymal hemorrhage.
CT appearance of acute appendagitis.

OBJECTIVE Our aim was to describe the spectrum of CT findings in patients with acute epiploic appendagitis and also to evaluate the changes seen with this condition. MATERIALS AND METHODS Fifty patients diagnosed with acute epiploic appendagitis seen on contrast-enhanced CT were included in this study. The CT scans of the epiploic appendagitis were evaluated for the presence of colon wall thickening, a focal fatty center, inflammatory changes, location in relationship to the colon, size, and presence or absence of central high density within the fat. In 10 patients, the initial findings were compared with findings of follow-up CT performed between 3 days-21 months after the first CT. RESULTS The most common part of colon involved by acute epiploic appendagitis was the sigmoid colon (31/50), and the most common position was anterior to the colonic lumen (41/50). All 50 patients with acute epiploic appendagitis had a central fatty core surrounded by inflammation. Colon wall thickening was present in only two, and a central high-density focus was noted only in 27 of 50 patients. In 86% (43/50) of patients, the fatty central core was between 1.5 and 3.5 cm in length. The changes seen on follow-up CT varied, including increased density with a decrease in the size of the fatty central core, no change, complete resolution of findings, and minimal residual density. CONCLUSION On CT, acute epiploic appendagitis has a predictable appearance in terms of location, size, and density. The most common finding on CT is a fat-density oval lesion with surrounding inflammation on the anterior aspect of the sigmoid colon. The changes on CT are not predictable in the 2-week to 6-month window.
Laxative Treatment With Polyethylene Glycol Does Not Affect Lipid Absorption in Rats

Objectives: Polyethylene glycol (PEG) is a frequently used laxative agent. It is unknown, however, whether PEG affects the absorptive capacity of the intestine. Reduced lipid (dietary fat and cholesterol) absorption induced by long-term PEG treatment could negatively affect growth in children. We tested whether PEG accelerates gastrointestinal transit and alters lipid absorption and plasma lipid levels. Methods: Wistar rats were administered drinking water with or without PEG (7%) for 2 weeks. We studied whole gut transit time by recording the first appearance of red feces after intragastric carmine red administration. We measured plasma concentrations of cholesterol and triglycerides, dietary fat absorption by 48-hour fat balance and by plasma appearance of intragastrically administered stable-isotope labeled fats, and cholesterol absorption with a dual stable isotope technique. Results: PEG decreased whole gut transit time by 20% (P = 0.028) without causing diarrhea. PEG treatment did neither affects overall dietary fat balance nor fat uptake kinetics, cholesterol absorption, or plasma lipid concentrations. Conclusions: PEG does not affect lipid absorption nor steady-state plasma lipid levels in rats, although it accelerates the gastrointestinal transit.
Updated features associated with type 1 gastric carcinoids patients: a single-center study

Abstract Objective. Data on clinical presentation and associated features of patients with type 1 gastric carcinoids (T1-GCs) are scanty. This study aimed to provide detailed data on a series of patients with T1-GCs. Material and Methods. Clinical, laboratory, endoscopic, and histological data were assessed from 31 T1-GCs patients (cross-sectional design), consecutively diagnosed in a tertiary center according to a standardized diagnostic protocol. T1-GCs were diagnosed at baseline or follow-up gastroscopy for atrophic gastritis in 74.2% and 25.8% of patients, respectively. Results. Seventy-one percent of T1-GC patients were female. Age ranged from 23 to 78 (median 58 years). T1-GCs were more frequently diagnosed between 40–49 years (35.5%) and 60–69 years (32.3%) (p = 0.0383). Thyroid disease was present in 54.8% (in 29% autoimmune). All 31 patients had either cobalamin or iron deficiency with or without anemia. Manifest pernicious anemia was present in 67.7% of patients and cobalamin deficiency without anemia in 9.7% patients. Iron deficiency anemia was present in 29% and iron deficiency without anemia in 12.9% of patients. In 48.4% of patients, T1-GCs appeared as polyps, which were single in all cases and had a median size of 4 mm (range 2–15 mm). In patients with polypoid T1-GCs, thyroid disease of autoimmune and nonautoimmune origin (p = 0.0181) was more frequently associated. Conclusion. This study shows that T1-GCs may be diagnosed at any age. Autoimmune features are frequently present as well as cobalamin and iron deficiency. The copresence of autoimmune diseases and micronutrient deficiencies should be accurately investigated, in particular in patients with polypoid T1-GCs.
Postural asymmetry in human stance: the mean center of pressure position

The aim of this study is to point out the influence of the feet position on the mean center of pressure (COP) position for a population of 25 healthy young right-handed men. Results have been compared to those of the literature. Most of them have been confirmed: mean COP position is on the subject's right side, that points out the asymmetry of the orthostatic posture. Moreover, the findings showed the feet position effects : when the clearance and/or the angle between the feet increase the mean COP moves backward and to the right.
Explaining Organizational Export Performance by Single and Combined International Business Competencies

This study explores relationships between export performance and international business competencies (international orientation, export market orientation and international entrepreneurial orientation), and interactions between the competencies. Data from on‐site structured interviews with 159 owners and managers of exporting firms from different economic sectors show direct relationships between the competencies and export performance. However, analyses of the effects of interactions between the competencies and export performance show mixed results. The findings suggest developing the identified competencies to increase export performance, but if this would be exclusively based on the direct relationships between the competencies and export performance, results may be suboptimal.
The most effective and promising population health strategies to advance human papillomavirus vaccination

ABSTRACT The US is failing to make substantive progress toward improving rates of human papillomavirus vaccine uptake. While the Healthy People 2020 goal for human papillomavirus (HPV) vaccination is 80%, the three-dose completion rate in the US in 2014 for 13- to 17-year-old females is less than 40%, and the rate for males is just above 20%. Experts point to a number of reasons for the poor HPV vaccination rates including parental concerns about safety, necessity, and timing. However, the evidence refuting these concerns is substantial. Efforts focusing on education and communication have not shown promise, but several population health strategies have reminder/recall systems; practice-focused strategies targeting staff, clinicians, and parents; assessment and feedback activities; and school-based HPV vaccination programs.
Dynamics of Early Childhood Overweight

Objective. To study the dynamic processes that drive development of childhood overweight by examining the effects of prenatal characteristics and early-life feeding (breastfeeding versus bottle feeding) on weight states through age 7 years. We test a model to determine whether prenatal characteristics and early-life feeding influence the development of a persistent early tendency toward overweight and/or whether prenatal characteristics and early-life feeding factors influence the likelihood that children will change weight states as they get older. Methods. Data from the National Longitudinal Survey of Youth’s Child-Mother file were used to implement these analyses. A total of 3022 children were included in this sample. For inclusion in this sample, valid information on height and weight during 3 consecutive interviews when the child was aged 24 to 95 months as well as valid data on prenatal and birth characteristics were needed. The primary outcome measure was childhood overweight (BMI >95th percentile). Multivariate logistic models and first-order Markov models were estimated. Results. Early development of childhood overweight was associated with race, ethnicity, maternal prepregnancy obesity, maternal smoking during pregnancy, and later birth years. In later years, the factor that contributed the most to being overweight was having been overweight in the previous observation period. However, with conditioning on the child’s having been overweight in the previous observation period, the prenatal factors that contributed to early childhood overweight, except for birth cohort, were also associated with development of overweight among children who had previously been normal weight and perpetuated the persistence of overweight over time. Conclusions. This research suggests that prenatal characteristics, particularly race, ethnicity, maternal smoking during pregnancy, and maternal prepregnancy obesity, exert influence on the child’s weight states through an early tendency toward overweight, which then is perpetuated as the child ages. These findings are intriguing as they provide additional clues to the genesis of childhood overweight and suggest that overweight prevention may need to begin before pregnancy and in early childhood.
AN OVERVIEW OF OCULAR ADNEXAL LYMPHOID TUMORS*

WHILE RESPECTABLE PROGRESS HAS BEEN MADE OVER THE PAST 10 TO 15 years in understanding many of the clinical and pathologic features of ocular adnexal lymphoid tumors, 1-16 definitive answers to several central biologic questions continue to elude us. Probably the most trenchant issue is: can one predict the ultimate fate of the patient, in terms of localized versus systemic disease, based on any feature(s) of the biopsy specimen that is obtained from the ocular adnexa? Ocular adnexal lymphoid lesions pose distinctive biologic problems because they are either extranodal or completely extralymphatic. There are no formed lymph nodes in the orbits, lacrimal glands, lids, or conjunctivas, so that all of these locations represent extranodal sites. The conjunctival7 and the lacrimal gland,'8 however, do possess lymphocytes and/or plasma cells, and therefore may be somewhat analogous to tonsillar or bowel tissue in terms of being extranodal yet lymphatic by offering a modified lymphoidal milieu. The orbit, on the other hand, lacks both a standing population of lymphocytes and endothelial-lined lymphatic channels; it truly represents an extralymphatic site, as indeed does the tissue of the lids. It is instructive to review from a telescoped historical perspective what some of our predecessors have written about the nettlesome subject of adnexal lymphoid lesions. It will become apparent that we should remain modest regarding our latter-day achievements because we have far from totally allayed their uncertainties. Friedenwald,19 in his 1929 text titled "The Pathology of the Eye," commented on both lymphosarcoma of the ocular adnexa and "inflammatory pseudotumors" in the following manner:
Managing local supplier networks: conflict or compromise?

ABSTRACT This paper examines conflict management in small firm networks. Informal conflict management strategies used in exchange relationships are identified and analysed. In-depth, semi-structured interviews with 22 small and medium-sized enterprise managers in an industrial district in the south-east of France are analysed. Results point to managers adopting accommodating behaviours in conflicts with clients and compromising and collaborative strategies with local partners. This research reveals the mobilization of local norms in the management of conflicts and also contributes to research concerning coopetition and the possibility that managers of small firms may both separate and integrate coopetition activities.
Characterization of a mammalian homolog of the Escherichia coli MutY mismatch repair protein

A protein homologous to the Escherichia coli MutY protein, referred to as MYH, has been identified in nuclear extracts of calf thymus and human HeLa cells. Western blot (immunoblot) analysis using polyclonal antibodies to the E. coli MutY protein detected a protein of 65 kDa in both extracts. Partial purification of MYH from calf thymus cells revealed a 65-kDa protein as well as a functional but apparently degraded form of 36 kDa, as determined by glycerol gradient centrifugation and immunoblotting with anti-MutY antibodies. Calf MYH is a DNA glycosylase that specifically removes mispaired adenines from A/G, A/7,8-dihydro-8-oxodeoxyguanine (8-oxoG or GO), and A/C mismatches (mismatches indicated by slashes). A nicking activity that is either associated with or copurified with MYH was also detected. Nicking was observed at the first phosphodiester bond 3' to the apurinic or apyrimidinic (AP) site generated by the glycosylase activity. The nicking activity on A/C mismatches was 30-fold lower and the activity on A/GO mismatches was twofold lower than that on A/G mismatches. No nicking activity was detected on substrates containing other selected mismatches or homoduplexes. Nicking activity on DNA containing A/G mismatches was inhibited in the presence of anti-MutY antibodies or upon treatment with potassium ferricyanide, which oxidizes iron-sulfur clusters. Gel shift analysis showed specific binding complex formation with A/G and A/GO substrates, but not with A/A, C.GO, and C.G substrates. Binding is sevenfold greater on A/GO substrates than on A/G substrates. The eukaryotic MYH may be involved in the major repair of both replication errors and oxidative damage to DNA, the same functions as those of the E. coli MutY protein.
Physiological glucocorticoid levels regulate glutamine and insulin-mediated glucose metabolism in skeletal muscle of the rat. Studies with RU 486 (mifepristone).

This study examined the effects of antagonism of the peak level of glucocorticoids in vivo, which occurs as rats enter the feeding/activity (dark) period on glucose and glutamine metabolism in incubated isolated rat soleus muscle preparations. Thus the rats were treated with the potent glucocorticoid antagonist RU 486 2 h before and 1 and 2 h into the dark period. Both the content of glutamine in skeletal muscle in vivo and plasma glucose and glutamine concentrations were elevated midway through the dark period, compared with the beginning of the period. RU 486 prevented the increases in plasma glucose and glutamine and caused a significant decrease in both the rate of release of glutamine in soleus muscle in vitro and the content of glutamine in gastrocnemius muscle. The sensitivity of soleus muscle to insulin in vitro is markedly decreased when isolated midway through the dark period (i.e. at 03:00 h) [Leighton, Kowalchuk, Challiss & Newsholme (1988) Am. J. Physiol. 255, E41-E45]. We now show that the concentrations of insulin required to stimulate lactate formation and glycogen synthesis half-maximally were 95 and 250 muunits/ml respectively, and treatment of rats with RU 486 decreased these values to 55 and 90 muunits of insulin/ml respectively. Thus antagonism of the action of the normal circadian rise in the level of glucocorticoids in rats reverses insulin insensitivity in soleus muscles in vitro.
Effects of vitamin A on immunological deficiencies in rats with obstructive jaundice.

OBJECTIVE To investigate the ability of an immunostimulant, vitamin A, to reverse dysfunction of the mononuclear phagocyte system and impaired peritoneal neutrophil chemotaxis in rats with obstructive jaundice. DESIGN Open laboratory study. SETTING Medical School, Turkey. MATERIAL 60 male Wistar-Albino rats. INTERVENTIONS Two different experimental studies with 30 rats each were performed. Ten of the 20 rats in which the common bile duct was ligated and divided, were given vitamin A (vitamin A group) and the other 10 were given saline (saline group). Ten rats which underwent laparotomy with mobilisation of the common bile duct (sham group) were given saline. Rats in the vitamin A group were given 200 IU/g/day vitamin A and other groups of rats had an equal volume of saline intraperitoneally for 20 days. MAIN OUTCOME MEASURES Function of the mononuclear phagocytic system was studied by the use of 99mTc sulphur colloid uptake, peritoneal neutrophil chemotaxis was measured by the Boyden chamber method, and liver function tests were studied 21 days after operation. RESULTS Hepatic uptake of 99mTc sulphur colloid decreased, and lung uptake increased in the saline group compared with the sham and vitamin A groups (p < 0.05). Neutrophil chemotaxis was reduced in the saline and vitamin A groups compared with the sham group (p < 0.05). Serum aspartate aminotransferase and alanine aminotransferase activities and unconjugated bilirubin concentrations in the saline group were higher than in the vitamin A and sham groups (p < 0.05). CONCLUSION Vitamin A stimulates mononuclear phagocytic function in jaundiced rats. It also improves liver function and may enhance peritoneal neutrophil chemotaxis.