Miracle-dz/UniFAR-FTUs huggingface dataset:
A NewVariant of Glanzmann ' s Thrombasthenia ( Strasbourg I ) Platelets with Functionally Defective Glycoprotein llb-Illa Complexes and a Glycoprotein Ilia 214 Arg-214 Trp Mutation

We describe a new variant of Glanzmann's thrombasthenia (variant Strasbourg I). The patient (M.S.) showed an absence of platelet aggregation to ADP, thrombin, and collagen, and a decreased clot retraction. Platelet fibrinogen was 20% of normal levels. ADP-stimulated platelets bound markedly reduced amounts of soluble fibrinogen and platelet adhesion to surface-bound fibrinogen was defective. Normal to subnormal amounts of glycoprotein (GP) Ilb-Illa (am,83) complexes, the platelet fibrinogen receptor, were revealed by SDS-PAGE, crossed immunoelectrophoresis, and antibody binding. However, the complexes were unusually sensitive to dissociation with EDTAat room temperature. Furthermore, flow cytometry showed that the platelets failed to bind the activation-dependent monoclonal antibody, PAC-1, after stimulation. In contrast, an RGDS-containing peptide induced significant binding of the anti-ligand-induced binding site antibody, D3GP3, suggesting the presence of a functional RGDbinding domain on the patient's GPIIb-IIIa complex. Sequence analysis was performed after polymerase chain reaction amplification of selected patient's GPIIIa exons, and of the patient's platelet GPIIb and GPIIIa mRNAs. A point mutation (C to T) was localized in exon D (iv) of GPIIIa that resulted in an 214Arg to 214Trp amino acid substitution. The defect has been inherited from the parents who are heterozygous for the same mutation. This substitution points to an essential amino acid in a region of GPIIIa involved in the binding of fibrinogen and influencing the Ca2+-dependent stability of the GPIIb-IIIa complex. (J. Clin. Invest. 1992. 89:1995-2004.)
Exports, imports and economic growth in South Korea and Japan: a tale of two economies

This article investigates the relationship between exports, imports and economic growth for South Korea and Japan by constructing a Vector Autoregression (VAR) model. Causality is examined between real Gross Domestic Product (GDP), real exports and real imports. Several principal results emerge from the empirical work. First, the three variables are cointegrated for both countries, implying that a long run steady state exists. Second, there is evidence of bidirectional causality between imports and economic growth for both countries. Finally, Japan seems to experience export-led growth, while GDP growth in South Korea has a negative effect on export growth. These contrasting findings could result from export goods in Japan exhibiting greater nonprice competitive aspects, although their success fails to trigger a virtuous circle since growth fails to lead to increased exports, while for South Korea, output growth leads to a decrease in export growth suggesting a strong domestic market.
Lymphocytes induce resorption of cartilage by producing catabolin.

Mononuclear cells from pig blood, when cultured in the presence of lectins (concanavalin A or phytohaemagglutinin), release a factor that induces resorption of proteoglycan in explants of live bovine nasal cartilage. The factor had mol.wt. 20000 and pI 4.6-5.0, and was indistinguishable from the cartilage-catabolic protein catabolin isolated from culture medium of explants of pig synovial tissue. Since much of the catabolin from the mononuclear cells arose from the non-adherent population (98% lymphocytes) it was concluded that lymphocytes (and possibly monocytes) make catabolin.
UvA-DARE ( Digital Academic Repository ) Are Spectral and Timing Correlations Similar in Different Spectral States in Black Hole X-Ray Binaries ?

We study the outbursts of the black hole X-ray binaries MAXI J1659-152, SWIFT J1753.5-0127, and GX 339-4 with the Swift X-ray Telescope (XRT). The bandpass of the XRT has access to emission from both components of the accretion flow: the accretion disk and the corona/hot flow. This allows a correlated spectral and variability study, with variability from both components of the accretion flow. We present for the first time a combined study of the evolution of spectral parameters (disk temperature and radius) and timing parameters (frequency and strength) of all power spectral components in different spectral states. Comparison of the correlations in different spectral states shows that the frequency and strength of the power spectral components exhibit dependencies on the disk temperature that are different in the (low-)hard and the hard-intermediate states (HIMSs); most of these correlations that are clearly observed in the HIMS (in MAXI J1659-152 and GX 339-4) are not seen in the (low-) hard state (in GX 339-4 and SWIFT J1753.5-0127). Also, the responses of the individual frequency components to changes in the disk temperature are markedly different from one component to the next. Hence, the spectral-timing evolution cannot be explained by a single correlation that spans both these spectral states. We discuss our findings in the context of the existing models proposed to explain the origin of variability.
Methylation of tRNAAsp by the DNA Methyltransferase Homolog Dnmt2

The sequence and the structure of DNA methyltransferase-2 (Dnmt2) bear close affinities to authentic DNA cytosine methyltransferases. A combined genetic and biochemical approach revealed that human DNMT2 did not methylate DNA but instead methylated a small RNA; mass spectrometry showed that this RNA is aspartic acid transfer RNA (tRNAAsp) and that DNMT2 specifically methylated cytosine 38 in the anticodon loop. The function of DNMT2 is highly conserved, and human DNMT2 protein restored methylation in vitro to tRNAAsp from Dnmt2-deficient strains of mouse, Arabidopsis thaliana, and Drosophila melanogaster in a manner that was dependent on preexisting patterns of modified nucleosides. Indirect sequence recognition is also a feature of eukaryotic DNA methyltransferases, which may have arisen from a Dnmt2-like RNA methyltransferase.
Technical aspects of minimal residual disease detection in carcinoma patients.

The burden of occult malignant cells which remains after a course of treatment that has resulted in clinical remission is referred to as minimal residual disease (MRD). MRD is increasingly considered as a determinant of local or systemic recurrence in cancer patients. During the last 20 years, methods for the detection of rare cancer cells have evolved from mere cytomorphological investigations to a variety of immunological and molecular assays. Since surgical therapy remains the best treatment option for cancer patients with resectable tumors, the first question to address is whether the removal of the tumor was complete or some cancer cells remained from the tumor at the primary site. Several tumor-associated DNA alterations have been identified to solve this diagnostic problem. Assays detecting tumor-associated DNA alterations have been applied to resection margins and body fluids such as bronchoalveolar lavage, sputum, urine, pancreatic juice, colonic lavage, and stool. Due to the higher sensitivity of immunocytochemical and reverse-transcriptase polymerase chain reaction (RT-PCR)-based assays, the second question to be addressed is whether systemic hematogenous or lymphatic spread of cancer cells occurred. Disseminated cancer cells have been detected in bone marrow aspirates, peripheral blood, and lymph node biopsies, and cancer cell dissemination is regarded as a relevant and independent prognostic factor. Thus, sensitive techniques for the detection of MRD are likely to guide indications for surgical or adjuvant therapy protocols in clinical oncology. However, since many of the assays for the detection of MRD are complex, and results are influenced by a variety of technical aspects, the majority of diagnostic applications have not yet been sufficiently standardized. Consequently, quality control and reproducibility of minimal disease detection assays remain unsolved problems. Therefore, well controlled collaborative studies are urgently required to evaluate indications and diagnostic standards for these assays. This review summarizes technical aspects and their implications for the clinical application of presently available assays for MRD detection in carcinoma patients.
The interactome of soybean GmWRKY53 using yeast 2-hybrid library screening to saturation

Soybean GmWRKY53 functions in both biotic and abiotic stress signaling. Using GmWRKY53 as a bait yeast 2-hybrid library screening to saturation isolated multiple independent fragments for many interacting proteins, enabling delineation of minimal interacting domains and computation of a confidence score. Multiple independent clones coding for the LATE ELONGATED HYPOCOTYL clock protein GmLCL2 (MYB114) were isolated and the binding site for GmWRKY53 was mapped to 90 amino acids separate from the MYB domain. This suggests a direct input from the clock on GmWRKY53 activity. The GmWRKY53-interacting proteins also included 3 water stress-inducible AP2/ERF transcription factors. One of these (Glyma03g26310) is one of the most strongly water stress induced genes in soybean roots, suggesting that GmWRKY53/ERF complexes regulate water stress responses.
Stable QPSK Demodulation Using a Digital Optical Phase-Locked Loop

We demonstrated stable error-free demodulation of 20-Gb/s Quaternary Phase-Shift-Keying (QPSK) for 80-km fiber transmission with a Digital Optical Phase Locked-Loop (DOPLL). We used a second order Active Loop Filter (ALF), which gave better stability than the first order ALF commonly used in conventional OPLLs, and optimized the LF constants. We theoretically compared the system stability with the second order ALF to that with the first order ALF and showed that the former had better phase margins under the assumption that they had the same loop bandwidth. This result indicates that the second order ALF is suitable for operation under noise attributed to the digital processing.
Secure beamforming and artificial noise design in interference networks with imperfect ECSI

This paper investigates the joint robust beamforming and artificial noise design in two-user interference networks, where one of the two pairs is wiretapped by an external eavesdropper, and only imperfect eavesdropper's channel state information (ECSI) is available for the two pairs. Under individual transmission power constraint, our goal is to maximize the worst-case achievable secrecy rate of the wiretapped node and guarantee the signal-to-interference-plus-noise ratio (SINR) requirement at the wiretap-free node. In particular, considering the channel vectors and channel covariance matrices errors, we first model the imperfect ECSI in the sense of ellipsoidal uncertainties. In the sequel, our design goal is transformed into solving a non-convex optimization problem which can be further reformulated as a two-stage problem. With the help of rank relaxation and one dimensional line search, the considered two-stage problem is solved efficiently. Simulation results demonstrate the effectiveness of the proposed design in different scenarios.
PRECISE OBJECT CONTOUR DETECTION USING VARIABLE BLOCK-BASED VIDEO FOREGROUND SEGMENTATION IN TRANSFORM DOMAIN

A precise object contour detection based on foreground segmentation in a video sequence is proposed. Our method employs a set of block-based Gaussian mixture models (GMM) on different block sizes, followed by foreground object contour extraction, which is based on Walsh transform (WT) domain. Every background model has different spatial and temporal correlations, and exploits WT-based feature parameters. This scheme can reduce both false positive errors and false negative errors, caused by dynamic background motions, such as global lighting changes and scenery in heavy snow. Moreover, a set of GMMs on every block size mode can be calculated by less than 10 % steps, compared with a conventional pixel-wise GMM scheme. The contour detection uses the result of the foreground segmentation for extracting precise edges along the actual contour by using WT horizontal/vertical spectra feature parameters of edge blocks. In addition to simple computation, our method requires no initialization with respect to boundaries of objects by using information of foreground regions. Experimental results show robust performance of the proposed method under outdoor conditions.
Stabilization Analysis of Sensorless IM Driver Control at Low Speed with Rr Estimator

This paper presents a novel technique of an sensor less indirect stator field-oriented IM (induction motor) drive speed control, which prevents the accumulative errors caused by the stator resistance is susceptible to temperature variations while conducting the flux estimation directly and computing the synchronous rotary speed. First, the research adds an adaptive flux observer to estimate the speed of the rotor. Secondly, using the fixed trace algorithm (FTA) to estimate the rotor resistance, thereby improving the problem of stability at the low speed in regenerating mode and the influence. Finally, the paper conducts simulations and practices of Simulink® of MATLAB® to verify the correctness of the result of the paper.
Biochemical adaptations in muscle. Effects of exercise on mitochondrial oxygen uptake and respiratory enzyme activity in skeletal muscle.

Abstract The capacity of the mitochondrial fraction from gastrocnemius muscle to oxidize pyruvate doubled in rats subjected to a strenuous program of treadmill running. Succinate dehydrogenase, reduced diphosphopyridine nucleotide dehydrogenase, DPNH cytochrome c reductase, succinate oxidase, and cytochrome oxidase activities, expressed per g of muscle, increased approximately 2-fold in hind limb muscles in response to the training. The concentration of cytochrome c was also increased 2-fold, suggesting that the rise in respiratory enzyme activity was due to an increase in enzyme protein. The total protein content of the mitochondrial fraction increased approximately 60%. These changes in the concentration of cytochrome c and total mitochondrial protein are of special interest because they suggest that exercise could serve as a useful tool for studying the biosynthesis of mitochondrial proteins. Mild exercise, such as that used in previous studies, was found to have no effect on the level of succinate dehydrogenase in muscle, suggesting that the failure of earlier studies to show an increase in respiratory enzyme activity resulted from the use of an insufficient exercise stimulus. Mitochondria from muscles of the exercised animals exhibited a high level of respiratory control and tightly coupled oxidative phosphorylation. Thus, the increase in electron transport capacity was associated with a concomitant rise in the capacity to produce adenosine triphosphate. This adaptation may partially account for the increase in aerobic work capacity that occurs with regularly performed, prolonged exercise.
RNA interference reveals a role for TLR2 and TLR3 in the recognition of Leishmania donovani promastigotes by interferon–γ‐primed macrophages

Leishmania donovani promastigotes evade the induction of a proinflammatory response during their invasion of naive macrophages. However, their entry into IFN‐γ‐primed macrophages is accompanied by the secretion of nitric oxide (NO) and proinflammatory cytokines. In the present study, we addressed the hypothesis that priming with IFN‐γ induces the expression of a receptor that enables mouse macrophages to recognize L. donovani promastigotes. We observed that in IFN‐γ‐primed macrophages, L. donovani promastigotes stimulated Interleukin‐1 receptor‐associated kinase‐1 (IRAK‐1) activity. We next showed that Toll‐like receptor (TLR)3 is barely detectable in naive macrophages but is expressed in IFN‐γ‐treated macrophages. Silencing of TLR3, TLR2, IRAK‐1 and myeloid differentiation factor 88 (MyD88) expression by RNA interference revealed that both TLR are involved in the secretion of NO and TNF‐α induced by L. donovani promastigotes. Using L. donovani mutants, we showed that TLR2‐mediated responses are dependent on Galβ1,4Manα‐PO4‐containing phosphoglycans, whereas TLR3‐mediated responses are independent of these glycoconjugates. Furthermore, our data indicate a participation of TLR2 and TLR3 in the phagocytosis of L. donovani promastigotes and a role for TLR3 in the leishmanicidal activity of the IFN‐γ‐primed macrophages. Collectively, our data are consistent with a model where recognition of L. donovani promastigotes depends on the macrophage activation status and requires the expression of TLR3.
A New Model-Based Technique for the Diagnosis of Rotor Faults in RFOC Induction Motor Drives

This paper proposes a new model-based diagnostic technique, which is the so-called virtual current technique (VCT), for the diagnosis of rotor faults in direct rotor field oriented controlled (DRFOC) induction motor drives. By measuring the oscillations at twice the slip frequency found in the rotor flux of the machine, and by conjugating this information with the knowledge of some motor parameters, as well as the parameters of the flux and current controllers, it is possible to generate a virtual magnetizing current which, after normalization, allows the detection and quantification of the extension of the fault. The proposed method allows one to overcome the major difficulties usually found in the diagnosis of rotor faults in closed-loop drives by providing information about the condition of the machine in a way that is independent of the working conditions of the drive such as the load level, reference speed, and bandwidth of the control loops. Although the VCT was primarily developed for traction drives used in railway applications, it can be incorporated in any DRFOC drive at almost no additional cost. Several simulation results, obtained with different types of DRFOC drives, as well as experimental results obtained in the laboratory, demonstrate the effectiveness of this new diagnostic approach.
Spiritual Accompaniment for Persons with Same-Sex Attraction

help given by one believer to another that enables the latter to pay attention to God’s personal communication to him or her, to respond to this personally communicating God, to grow in intimacy with this God, and to live out the consequences of the relationship. The focus of this type of spiritual direction is on experience [. ] Moreover, this experience is viewed, not as an isolated event, but as an expression of the ongoing personal relationship God has established with each one of us. (Barry and Connolly 1982, 8–9)
Susceptibility of Mice to Bacterial and Fungal Infections after Intragastric Administration of Ebselen

The seleno‐organic compound ebselen (2−phenyl−1,2−benzisoselenazol−3(2H)‐one) has anti‐inflammatory activity and exhibits glutathione peroxidase‐like activity in‐vitro. Ebselen inhibited candidacidal activity over the same range of concentrations as it inhibited the production of microbicidal H2O2 by human neutrophils and macrophage‐like cells. Therefore, the long‐term administration of ebselen might be expected to induce an immunocompromised state in the host. To examine such a possibility, mice (5−weeks‐old ddY, male) were given daily intragastric doses of 0, 10 or 100 mg kg−1 ebselen for 21 days and then infected intraperitoneally with Candida albicans (108 cells/mouse), Pseudomonas aeruginosa (1.5 times 107 cells/mouse) or methicillin‐resistant Staphylococcus aureus (5 times 108 cells/mouse). Ebselen at none of the tested doses affected the increase in body weight of mice during administration of the drug. No evidence was obtained that mice became more susceptible to the various microorganisms after the administration of ebselen at any tested dose.
Visual attention in preschool children prenatally exposed to cocaine: Implications for behavioral regulation

The presence of cocaine during the prenatal period disrupts the development of neural systems involved in mediating visual attention; therefore, it is possible that prenatal cocaine exposure results in impairments in visual attention in early childhood. In the current study we hypothesized that preschool children with prenatal cocaine exposure would exhibit difficulties in the disengagement operation of visual attention and in sustaining attention, particularly for targets presented in the right visual field. Fourteen cocaine-exposed children and 20 control children between 14 and 60 months of age were assessed on measures of visual attention, cognition, and behavior. Cocaine-exposed children had slower reaction times on disengagement trials in the second half of our attention task, supporting our hypotheses that impairments in disengagement and sustained attention are associated with prenatal cocaine exposure. There was a trend for slower reaction times to targets presented in the right visual field, but not to targets presented in the left visual field. Cocaine-exposed children also exhibited greater difficulties in behavioral regulation. Overall, our findings suggest that children with prenatal cocaine exposure demonstrate specific impairments in visual attention and behavioral regulation. (JINS, 2002, 8, 12–21.)
Architectural review of polynomial bases finite field multipliers over GF(2m)

In elliptic curve cryptography (ECC), hardware architectures of finite field (FF) multipliers are frequently proposed for polynomial as well as for normal bases representations over GF(2m). Although the polynomial bases provide efficient FF multiplication as compared to normal bases, the performance of the entire elliptic cryptosystem mainly depends upon its FF multiplier. Consequently, this paper provides a comparative overview of the recent hardware architectures of FF multipliers for polynomial bases over GF(2m). This is achieved by classifying the most recent state-of-the-art research practices into three categories: bit-serial, bit-parallel and digit-serial multipliers. The comparison of multiple techniques in this article enables the designer to select a suitable multiplier according to different application requirements such as high speed/performance, constrained environments and high throughput/area applications.
Self-Oscillating Tuning Loops for Series Resonant Inductive Power Transfer Systems

This paper presents power and frequency tuning loops based on fundamental harmonic operation (FHO) and third harmonic operation (THO) for inductive power transfer (IPT) systems. THO is an effective solution for applications requiring high-frequency power supply with low voltage gains, e.g., low-voltage contactless battery chargers. Moreover, it can reduce the switching losses and increase the net efficiency of IPT systems in light-load conditions. Compared to conventional methods, such as phase-locked-loop (PLL) circuits, the proposed methods are less affected by misalignments and instantly track the resonant frequency. Hence, they can be utilized in self-switched capacitor and variable coupling applications. In addition, the new tuning systems can switch between THO and FHO modes for different loadings, voltage gains, and couplings conditions, e.g., dynamic powering of different moving pickups. To show the performance of the new tuning loops, laboratory prototypes with switching frequency of 20-50 kHz are implemented based on half-bridge and full-bridge inverters.