Miracle-dz/UniFAR-FTUs huggingface dataset:
The Physiological Experimental Study on the Effect of Different Color of Safety Signs on a Virtual Subway Fire Escape—An Exploratory Case Study of Zijing Mountain Subway Station

Safety signs play a very important role in people’s evacuation during emergencies. In order to explore the appropriate color for subway safety signs, four safety signs of different color combinations are designed, and the virtual reality, eye-tracking technology, and physiological indicator measurement are used in a virtual subway fire escape experiment. A total of 96 participants with equal distribution in gender and four different color combination groups were recruited. Participants’ eye-tracking and physiological data (heart rate, skin conductance) were real-time recorded through ErgoLAB V3.0 in the whole experiment. The relationship between Color_of_safety_sign and escape performance, eye-tracking indicators, and physiological indicators is discussed respectively through SPSS. The results show that “Green and black” group has the best evacuation escape performance, low cognitive load, high search efficiency on safety signs, and the highest stress level and immersion and “Green and black” can be the most appropriate color for safety sign. This research is of certain significance for improving the function of subway fire-fighting infrastructure and the resilience of the metro system. Moreover, it can provide references and advice on risk management, emergency evacuation, and so on.
Temperature and photoperiod effects on dormancy status and life cycle parameters in Aedes albopictus and Aedes aegypti from subtropical Argentina

Aedes albopictus (Diptera: Culicidae) distribution is bounded to a subtropical area in Argentina, while Aedes aegypti (Diptera: Culicidae) covers both temperate and subtropical regions. We assessed thermal and photoperiod conditions on dormancy status, development time and mortality for these species from subtropical Argentina. Short days (8 light : 16 dark) significantly increased larval development time for both species, an effect previously linked to diapause incidence. Aedes albopictus showed higher mortality than Ae. aegypti at 16 °C under long day treatments (16 light : 8 dark), which could indicate a lower tolerance to a sudden temperature decrease during the summer season. Aedes albopictus showed a slightly higher percentage of dormant eggs from females exposed to a short day, relative to previous research in Brazilian populations. Since we employed more hours of darkness, this could suggest a relationship between day‐length and dormancy intensity. Interestingly, local Ae. aegypti presented dormancy similar to Ae. albopictus, in accordance with temperate populations. The minimum dormancy in Ae. albopictus would not be sufficient to extend its bounded distribution. We believe that these findings represent a novel contribution to current knowledge about the ecophysiology of Ae. albopictus and Ae. aegypti, two species with great epidemiological relevance in this subtropical region.
Is the Haugh Unit Correction for Egg Weight Valid for Eggs Stored at Room Temperature

Abstract The Haugh unit (HU) is the measure of albumen quality used by the poultry industry, but its correction for egg weight has been severely criticized. The validity of the correction for egg weight was investigated with an unwashed, unclassified sample of 800 eggs obtained from a commercial egg classification station. This sample was divided into four groups, one for study immediately and the others after storage at room temperature for 1, 2, or 3 wk. After weighing, each egg was broken and the height of the albumen was measured. Weights of the yolk, shell, and albumen were determined for each egg, and albumen pH was determined from pooled samples of three eggs each. Haugh units were calculated using the formula: HU = 100 log[H + 7.57 − 1.7W. 37]. Statistical relationships between these variables were investigated. A weak relation (r2 = .04 to .07) between albumen height and egg weight was observed. Haugh unit score was dependent on albumen height (r2 = .88 to .95) but independent of either egg or albumen weight, except after 3 wk of storage and when all data were combined. Time in storage explained 78% of the variation in HU and 77% of the variation in albumen height. A regression model including both week and egg weight did not increase these R2 values. Changes in albumen quality over time in storage were described equally well by albumen height as they were by the HU score, suggesting that the HU correction for egg weight is unnecessary.
A High-Strength Strain Sensor Based on a Reshaped Micro-Air-Cavity

We demonstrate a high-strength strain sensor based on a micro-air-cavity reshaped through repeating arc discharge. The strain sensor has a micro-scale cavity, approximate plane reflection, and large wall thickness, contributing to a broad free spectrum range ~36 nm at 1555 nm, high fringe contrast ~38 dB, and super-high mechanical robustness, respectively. A sensitivity of ~2.39 pm/με and a large measurement range of 0 to 9800 με are achieved for this strain sensor. The strain sensor has a high strength, e.g., the tensile strain applied the sensor is up to 10,000 με until the tested the single-mode fiber is broken into two sections. In addition, it exhibited low thermal sensitivity of less than 1.0 pm/°C reducing the cross-sensitivity between tensile strain and temperature.
THE ECOLOGY OF LOUGH INE XVI. PREDATION AND DIURNAL MIGRATION IN THE PARA CENTROTUS COMMUNITY

In various parts of Lough Ine the sea urchin Paracentrotus lividus Lamarck populates the shallow sublittoral from low water down to about 1 m below this level, and keeps the bottom clear of algae by its grazing (Kitching & Ebling 1961). The most extensive areas regularly occupied by Paracentrotus are along the north shore of the lough, on boulders and on solid rock, and in Curlew Bay, on shell gravel fringed at low water mark with rocks. The sea bed slopes gently at these localities, and predators are few. Of the larger crabs only Carcinus occurs commonly, and starfish normally remain in slightly deeper water. On the other hand, filter feeders are present in large numbersPomatoceros triqueter (L.) (Polychaeta), Anomia ephippium L. (Lamellibranchia) and (less abundantly) Chlamys varia (L.) (Lamellibranchia). There are also substantial numbers of the grazing gastropod Gibbula cineraria (L.). New patches, dominated by Paracentrotus and bare of algae, have recently developed in many parts of the lough, and especially in the South Basin. In these places the shore is steep and heaped with boulders. There are additional predators-the starfish Marthasterias glacialis (L.), Asterias rubens L. and Asterina gibbosa (Pennant) under the boulders, the crab Portunus puber (L.) as a nocturnal visitor, and occasionally also the edible crab, Cancer pagurus L. Grazing and filter-feeding molluscs are much less abundant. This paper is concerned with the ecological inter-relations of important members of the Paracentrotus community. We have attempted to formulate the system of food chains operating within the community, and to interpret diurnal migrations of some of its members in relation to predation.
Adenovirus-Mediated FasL Minigene Transfer Endows Transduced Cells with Killer Potential

Fas ligand (First apoptosis signal ligand, FasL, also known as CD95L) is the common executioner of apoptosis within the tumor necrosis factor (TNF) superfamily. We aimed to induce functional FasL expression in transduced cells using an adenovirus vector, which has the advantage of strong and transient induction of the gene included in the adenoviral genome. Here, we report that the adenovirus carrying a truncated FasL gene, named FasL minigene, encoding the full-length FasL protein (Ad-gFasL) is more efficient than the adenovirus carrying FasL cDNA (Ad-cFasL) in the induction of FasL expression in transduced cells. FasL minigene (2887 bp) lacking the second intron and a part of the 3′-UTR was created to reduce the gene length due to the size limitation of the adenoviral genome. The results show that, in transduced hepatocytes, strong expression of mRNA FasL appeared after 10 h for Ad-gFasL, while for Ad-cFasL, a faint expression appeared after 16 h. For Ad-gFasL, the protein expression was noticed starting with 0.5 transfection units (TU)/cell, while for Ad-cFasL, it could not be revealed. FasL-expressing endothelial cells induced apoptosis of A20 cells in co-culture experiments. FasL-expressing cells may be exploitable in various autoimmune diseases such as graft-versus-host disease, chronic colitis, and type I diabetes.
HONEYBEE COLONY WEIGHT AS AN INDEX OF HONEY PRODUCTION AND NECTAR FLOW: A CRITICAL EVALUATION

SUMMARY Honeybee colony weight (C(g)) was related to honey stored (H(g)), but this relationship was not linear due to an increased population of mature and immature bees in the middle of the active season. Honey stored was shown to be dependent on the number of days (D) from the beginning of the honey harvest. These factors were related for the study area by the polynomial: H = -1416O0+O07604 C- 57-142 D+0-487 D2+0-00142 CD which accounted for 97% of the total variation. Using the relationship between colony weight and honey stored, nectar flows in two different districts (coastal and upland) were characterized over four seasons and colony weights were different from one another in June and again in August and September, probably due to available moisture and temperature.
A Simple and Stable Centeredness Measure for 3D Curve Skeleton Extraction

Existing methods for extracting 3D curve skeletons mostly suffer from the difficulty of finding the center points of 3D shapes and tedious manual adjustments of the thresholds for pruning spurious branches due to the influence of shape boundary perturbations. In this article, we present a method based on medial surfaces of 3D shapes for the convenient and fast extraction of high-quality curve skeletons. Our main contribution is a simple and stable centeredness measure. It is based on simulating fire propagation via the scheme of inside-out evolution from the interior to the boundary, differentiating it from existing methods that use the scheme of outside-in evolution from the boundary to the interior. Thus, our measure is much more localized, and it can be implemented with a high degree of parallelism. In addition, we propose measures to effectively suppress the influence of details to obtain a stable measurement, and employ minimum set covers to optimize the center points to generate compact skeletons, which enables spurious branches to be effectively excluded without the tedious work of manually adjusting thresholds. Our experiments show the superiority of our method over existing methods, including its convenient generation of clean, compact and centered curve skeletons while running much faster than state-of-the-art methods.
Porphyromonas gulae lipopolysaccharide elicits inflammatory responses through toll‐like receptor 2 and 4 in human gingivalis epithelial cells

Porphyromonas gulae, a Gram‐negative black‐pigmented anaerobe, has been associated with periodontal disease in companion animals and its virulence has been attributed to various factors, including lipopolysaccharide (LPS), protease and fimbriae. Toll‐like receptors (TLRs) recognise pathogen‐associated molecular patterns, such as peptidoglycan, lipids, lipoproteins, nucleic acid and LPS. Following P. gulae infection, some inflammatory responses are dependent on both TLR2 and TLR4. In addition, a recent clinical study revealed that acute and persistent inflammatory responses enhance the expressions of TLR2 and TLR4 in the oral cavity. In this study, we investigated the interaction between P. gulae LPS and human gingivalis epithelial cells (Ca9‐22 cells). P. gulae LPS was found to increase TLR2 and TLR4 mRNA expressions and protein productions, and enhanced inflammatory responses, such as COX2, TNF‐ɑ, IL‐6 and IL‐8. Stimulated Ca9‐22 cells exhibited phosphorylation of ERK1/2 and p38, and their inhibitors diminished inflammatory responses, while knockdown of the TLR2 and/or TLR4 genes with small interfering RNA (siRNA) prevented inflammatory responses. Moreover, p38 and ERK1/2 phosphorylation was decreased in TLR2 and TLR4 gene knockdown cells. These findings suggest that P. gulae LPS activates p38 and ERK1/2 via TLR2 and TLR4, leading to inflammatory responses in human gingival epithelial cells.
Magnetically Functionalized Moss Biomass as Biosorbent for Efficient Co2+ Ions and Thioflavin T Removal

Microwave synthesized iron oxide nanoparticles and microparticles were used to prepare a magnetically responsive biosorbent from Rhytidiadelphus squarrosus moss for the rapid and efficient removal of Co2+ ions and thioflavin T (TT). The biocomposite was extensively characterized using Fourier transformed infrared (FTIR), XRD, SEM, and EDX techniques. The magnetic biocomposite showed very good adsorption properties toward Co2+ ions and TT e.g., rapid kinetics, high adsorption capacity (218 μmol g−1 for Co and 483 μmol g−1 for TT), fast magnetic separation, and good reusability in four successive adsorption–desorption cycles. Besides the electrostatic attraction between the oxygen functional moieties of the biomass surface and both Co2+ and TT ions, synergistic interaction with the –FeOH groups of iron oxides also participates in adsorption. The obtained results indicate that the magnetically responsive biocomposite can be a suitable, easily separable, and recyclable biosorbent for water purification.
Intramuscular Mechanisms Mediating Adaptation to Low-Carbohydrate, High-Fat Diets during Exercise Training

Interest in low-carbohydrate, high-fat (LCHF) diets has increased over recent decades given the theorized benefit of associated intramuscular adaptations and shifts in fuel utilization on endurance exercise performance. Consuming a LCHF diet during exercise training increases the availability of fat (i.e., intramuscular triglyceride stores; plasma free fatty acids) and decreases muscle glycogen stores. These changes in substrate availability increase reliance on fat oxidation for energy production while simultaneously decreasing reliance on carbohydrate oxidation for fuel during submaximal exercise. LCHF diet-mediated changes in substrate oxidation remain even after endogenous or exogenous carbohydrate availability is increased, suggesting that the adaptive response driving changes in fat and carbohydrate oxidation lies within the muscle and persists even when the macronutrient content of the diet is altered. This narrative review explores the intramuscular adaptations underlying increases in fat oxidation and decreases in carbohydrate oxidation with LCHF feeding. The possible effects of LCHF diets on protein metabolism and post-exercise muscle remodeling are also considered.
Purification and Properties of Methanol Dehydrogenase and Aldehyde Dehydrogenase from Methylobacillus glycogenes

Methanol dehydrogenase (MDH) and aldehyde dehydrogenase (ALDH) were purified to a homogenous state from Methylobacillus glycogenes, an obligate methylotroph. MDH (Mr 140,000) was composed of two different subunits (Mr 60,000 and 9,000) forming an α2β2 structure. MDH was indicated as a metalloquinoprotein containing one atom of calcium (Ca) per enzyme molecule. Binding of Ca was so tight that it was hard to remove Ca completely without denaturation of enzyme protein. A partially resolved enzyme resumed its original enzyme activity upon exogenous addition of Ca. Purified ALDH (Mr 144,000) was composed of two identical subunits of molecular mass of 72,000. ALDH was proved to be a quinoprotein in which PQQ is bound covalently.
Characterization of the Relationship between the Chaperone and Lipid-Binding Functions of the 70-kDa Heat-Shock Protein, HspA1A

HspA1A, a molecular chaperone, translocates to the plasma membrane (PM) of stressed and cancer cells. This translocation results in HspA1A’s cell-surface presentation, which renders tumors radiation insensitive. To specifically inhibit the lipid-driven HspA1A’s PM translocation and devise new therapeutics it is imperative to characterize the unknown HspA1A’s lipid-binding regions and determine the relationship between the chaperone and lipid-binding functions. To elucidate this relationship, we determined the effect of phosphatidylserine (PS)-binding on the secondary structure and chaperone functions of HspA1A. Circular dichroism revealed that binding to PS resulted in minimal modification on HspA1A’s secondary structure. Measuring the release of inorganic phosphate revealed that PS-binding had no effect on HspA1A’s ATPase activity. In contrast, PS-binding showed subtle but consistent increases in HspA1A’s refolding activities. Furthermore, using a Lysine-71-Alanine mutation (K71A; a null-ATPase mutant) of HspA1A we show that although K71A binds to PS with affinities similar to the wild-type (WT), the mutated protein associates with lipids three times faster and dissociates 300 times faster than the WT HspA1A. These observations suggest a two-step binding model including an initial interaction of HspA1A with lipids followed by a conformational change of the HspA1A-lipid complex, which accelerates the binding reaction. Together these findings strongly support the notion that the chaperone and lipid-binding activities of HspA1A are dependent but the regions mediating these functions do not overlap and provide the basis for future interventions to inhibit HspA1A’s PM-translocation in tumor cells, making them sensitive to radiation therapy.
Molecular Biomarkers in Multiple Sclerosis and Its Related Disorders: A Critical Review

Multiple sclerosis (MS) is a chronic autoimmune disease affecting the central nervous system (CNS) which can lead to severe disability. Several diseases can mimic the clinical manifestations of MS. This can often lead to a prolonged period that involves numerous tests and investigations before a definitive diagnosis is reached. As well as the possibility of misdiagnosis. Molecular biomarkers can play a unique role in this regard. Molecular biomarkers offer a unique view into the CNS disorders. They help us understand the pathophysiology of disease as well as guiding our diagnostic, therapeutic, and prognostic approaches in CNS disorders. This review highlights the most prominent molecular biomarkers found in the literature with respect to MS and its related disorders. Based on numerous recent clinical and experimental studies, we demonstrate that several molecular biomarkers could very well aid us in differentiating MS from its related disorders. The implications of this work will hopefully serve clinicians and researchers alike, who regularly deal with MS and its related disorders.
Clustering of Dietary Patterns and Lifestyles Among Spanish Children in the EsNuPI Study †

Dietary patterns (DPs) are known to be tied to lifestyle behaviors. Understanding DPs and their relationships with lifestyle factors can help to prevent children from engaging in unhealthy dietary practices. We aimed to describe DPs in Spanish children aged 1 to <10 years and to examine their associations with sociodemographic and lifestyle variables. The consumption of toddler and young children milk formulas, enriched and fortified milk within the Spanish pediatric population is increasing, and there is a lack of evidence whether the consumption of this type of milk is causing an impact on nutrient intakes and if they are helping to reach the nutrient recommendations. Within the Nutritional Study in the Spanish Pediatric Population (EsNuPI), we considered two study cohorts and three different age groups in three year-intervals in each of them. The study cohort included 740 children in a representative sample of the urban non-vegan Spanish population and 772 children in a convenience cohort of adapted milk consumers (AMS) (including follow-on formula, toddler’s milk, growing up milk, and fortified and enriched milks) who provided information about sociodemographics, lifestyle, and dietary habits; a food frequency questionnaire was used for the latter. Principal component analysis was performed to identify DPs from 18 food groups. Food groups and sociodemographic/lifestyle variables were combined through a hierarchical cluster algorithm. Three DPs predominated in every age group and study sample: a palatable energy-dense food dietary pattern, and two Mediterranean-like DPs. However, children from the AMS showed a predominant dietary pattern markedly related to the Mediterranean diet, with high consumption of cereals, fruits and vegetables, as well as milk and dairy products. The age of children and certain lifestyle factors, namely level of physical activity, parental education, and household income, correlated closely with the dietary clusters. Thus, the findings provide insight into designing lifestyle interventions that could reverse the appearance of unhealthy DPs in the Spanish child population.
Pars plana vitrectomy in uveitis in the era of microincision vitreous surgery

Pars plana vitrectomy (PPV) in uveitis is indicated for various diagnostic and therapeutic indications. With the advent of microincision vitreous surgery (MIVS), the use of PPV in uveitis has increased with a wider spectrum of indications due to shorter surgical time, less patient discomfort, less conjunctival scarring, and a decreased rate of complications as compared to standard 20G vitrectomy. Because of faster post-operative recovery in terms of visual improvement and reduction of inflammation, and reduced duration of systemic corticosteroids, MIVS has gained popularity in uveitis as an adjunctive therapy to the standard of care medical therapy. The safety and efficacy of MIVS is related to the emerging vitrectomy techniques with better and newer cutters, illuminating probes, and accessory instruments. Because of the instrumentation and fluidics of MIVS, PPV is emerging as a safe and useful alternative for diagnostic challenges in uveitis, aiding in earlier diagnosis and better outcome of inflammatory disease, even in the presence of severe and active inflammation, which was once considered a relative contraindication for performing vitreous surgery. However, for surgical interventions for therapeutic indications and complications of uveitis, it is advisable to achieve an optimum control of inflammation for best results. The increasing reports of the use of MIVS in uveitis have led to its wider acceptance among clinicians practicing uveitis.
Diverse Roles for Hyaluronan and Hyaluronan Receptors in the Developing and Adult Nervous System

Hyaluronic acid (HA) plays a vital role in the extracellular matrix of neural tissues. Originally thought to hydrate tissues and provide mechanical support, it is now clear that HA is also a complex signaling molecule that can regulate cell processes in the developing and adult nervous systems. Signaling properties are determined by molecular weight, bound proteins, and signal transduction through specific receptors. HA signaling regulates processes such as proliferation, differentiation, migration, and process extension in a variety of cell types including neural stem cells, neurons, astrocytes, microglia, and oligodendrocyte progenitors. The synthesis and catabolism of HA and the expression of HA receptors are altered in disease and influence neuroinflammation and disease pathogenesis. This review discusses the roles of HA, its synthesis and breakdown, as well as receptor expression in neurodevelopment, nervous system function and disease.