Miracle-dz/UniFAR-FTUs huggingface dataset:
Elucidating the role of Cdc25A in hypoxia-mediated cell cycle arrest

Hypoxia represents an important element of the solid tumor microenvironment and contributes to tumorigenesis and resistance to chemo- and radiation therapy. Hypoxia can modulate cell cycle progression although the mechanisms involved remain unclear. The Cdc25A dual specificity phosphatase promotes cell cycle progression by dephosphorylating and activating cyclin-dependent kinases. Cdc25A is the master regulator of the cell cycle and its disruption induces cell cycle arrest in cancer cells. The recent observation that under hypoxic conditions, levels of Cdc25A protein and mRNA are decreased led to the hypothesis that hypoxia-mediated reduction in Cdc25A may represent a novel mechanism in the hypoxic regulation of the cell cycle. Given the prominent role of Cdc25A in regulating the cell cycle and the proposed changes in Cdc25A protein and mRNA under hypoxic conditions, it was hypothesized that Cdc25A plays an essential role in hypoxia-mediated cell cycle arrest in human tumor cells. The specific aims were to: 1) examine the mechanism of Cdc25A downregulation in response to hypoxia, 2) determine the role HIF-1α in Cdc25A regulation and cell cycle arrest, and 3) determine if Cdc25A downregulation is required for hypoxia-induced cell cycle arrest. Under hypoxic conditions, Cdc25A protein levels were specifically and reversibly suppressed. It was found that Cdc25A mRNA levels are significantly decreased by a p21-dependent mechanism. In addition, suppression of Cdc25A was independent of p53. Loss of Cdc25A protein occurred in the absence of checkpoint activation. Recent evidence has linked the microRNA miR-21 to Cdc25A and hypoxia. It is shown here that miR-21 was required for Cdc25A mRNA suppression in hypoxic colon cancer cells and miR-21 levels were increased under hypoxic conditions. The HIF-1α transcription factor was not required for suppression of Cdc25A but must be present for hypoxia-induced cell cycle arrest. Under hypoxic conditions, cells undergo p21- and miR-21- S-phase cell cycle arrest. This study proposes a novel mechanism of transient regulation of Cdc25A via the p21- and miR-21-dependent regulation of mRNA levels in hypoxic cells leading to cell cycle arrest. This previously unknown mechanism may confer protection from hypoxic conditions, contributing to cell survival and the observed resistance to chemo- and radiation therapy.
Large-Scale Hydrodynamic Simulations of Astrophysical Jets

We present results of fully three-dimensional (3-D) simulations of astrophysical jets. These ef- forts use the the PLUTO code (Mignone et al. 2007) run in a highly parallel environment for the hydrodynamic, magneto-hydrodynamic (MHD), relativistic hydrodynamic (RHD), and relativis- tic, magnetohydrodynamic (RMHD) simulations. In this work, we focus on RMHD simulations, but also present estimations of the 15 GHz radio flux based on RHD simulations post-processed to show the radio-emission from the astrophysical jets. Keywords: jets, active galaxies, blazars, intracluster medium, non-linear dynamics, plasma as- trophysics, computational fluid dynamics, relativistic, magnetized fluid flows.
Immediate Effect of Quadriceps Elastic Taping Application as a Supplementary Treatment on Gait Performance in Patients with Knee Osteoarthritis: A Serial Case Report

: Quadriceps strength is an important determinant of walking in subjects with knee OA (KOA). Quadriceps Elastic Taping (ET) application might immediately improve gait performance while other modalities may need time to show improvement. Case Description: Seven patients with KOA (9 knees) presented with pain during weight bearing. ET was prescribed as a supplementary treatment to improve gait performance. Increased gait performance such as walking speed and reduced excessive knee flexion during loading response was seen in 77% and 66% of subjects respectively. In order to evaluate changes in muscle contraction immediately after ET application, surface EMG examination was done and we found only 55% of subjects have increased percentage of maximum voluntary contraction (MVC). Discussion: Increased cutaneous input during ET application in the afferent nerves may activate a loop via afferent and efferent nerve fibres which eventually increasing quadriceps muscle activation that may reduce joint load during gait. Unexpectedly, increased quadriceps contraction after ET application was inconsistent, therefore placebo effect may also play an important role in developing the findings in this study. Conclusion: ET application have potential to improve gait performance in patients with KOA. Further studies are needed to prove ET effect on gait performance.
The Long and the Short of it: Testing the Conversion and Cuckold Strategies of Ancestral Human Outgroup Mating

The human social group likely aided in ancestral human’s survival. However, the small-knit extended kin group in which human ancestors evolved posed a plausible reproductive threat in the form of inbreeding. The outgroup mating hypothesis (Salvatore, Meltzer, & Gaertner, under review) proposed that, as a solution to the inbreeding dilemma, ancestral females may have mated outside their social group. The current work examines two competing hypotheses by which ancestral females mated with outgroup males and balanced parental investment concerns. The conversion hypothesis posits that ancestral females mated with an outgroup male under the provision that he and his group would care for the offspring. The cuckold hypothesis proposes that ancestral females furtively mated with an outgroup male while retaining primary partner and group support for the offspring. The current work uses multiple methods and measures to test the competing hypotheses against one another. Study 1 manipulates women’s motivational state to reflect a short-term sexual (i.e., one-night-stand) or long-term committed (i.e., marriage) mate-seeking strategy and measures attraction to ingroup and outgroup men. Study 2 uses a restricted response window and assesses evaluations of ingroup and outgroup men for sex and marriage partners. Study 3 uses a version of the affect misattribution procedure (Payne et al., 2006). Results of Study 1 indicated strong support for the conversion hypothesis. Results of Study 2 and Study 3 were inconclusive. I suggest that ancestral women who mated with an outgroup man and converted to his collective received a fitness benefit over ancestral women who did not. Implications of results and future directions are discussed.
Drying effect on the properties of traditionally processed sago starch

Local people in Sarawak, Malaysia produce sago starch, commonly referred as lemantak, using traditional method for authentic meals and delicacies. The quality of lemantak degrades with time due to its high moisture content limiting the potential for a wider market, and hence affecting the socio-economy of those whose livelihood depends on sago starch production. The objective of the present work was to evaluate the changes in the properties of traditionally processed dried Sarawak sago starch. In order to achieve this, sago starch was extracted using a well-established traditional process and was dried at 40°C to produce sago starch with moisture contents of 40%, 30%, 20% and 10% wet basis. The effect of moisture content on the physical properties was studied through colour analysis, microscopic analysis, and particle size distribution. Analysis on resistant starch content was also performed. Changes on the hydration and functional properties was monitored by measuring the water absorption index (WAI), water solubility index (WSI), swelling capacity (SC), and gelatinisation behaviour. Lastly, Fourier transform-infrared spectroscopy (FT-IR) was applied to observe the changes in amorphous and crystalline areas. The physical properties analysis showed changes in starch colour and granule surface; but the change on granule size varied. Dried starch with lower moisture content exhibited higher resistant starch, absorption index, and peak temperature, but lower solubility index, swelling capacity, peak viscosity, crystalline index, and amorphous index. It is suggested that moisture content affected the changes in traditionally processed sago starch properties which was influenced by few components namely polyphenol, lipid, amylose-lipid complex, and inter-molecular hydrogen bond.
KERAGAMAN GENETIK KEDELAI BERDASARKAN POLA PITA DNA HASIL RAPD (Random Amplified Polymorphic DNA)

Informasi keragaman genetik plasma nutfah kedelai sangat diperlukan dalam program pemuliaan tanaman. Cara termudah untuk mempelajari keragaman genetik adalah dengan mengamati karakter morfologi. Akan tetapi, beberapa karakter morfologi dipengaruhi oleh faktor lingkungan. Sebaliknya, mempelajari keragaman genetik dengan pengamatan molekul, yaitu berdasar penanda DNA, dapat memberikan hasil yang lebih baik, konsisten, dan tidak dipengaruhi oleh faktor lingkungan maupun tahap perkembangan tanaman. RAPD (Random Amplified Polymorphic DNA) merupakan salah satu penanda DNA yang dapat digunakan untuk menganalisis keragaman genetik dan hubungan kekerabatan antargenotip. Penelitian ini bertujuan untuk mengetahui keragaman genetik dan kekerabatan 50 genotip kedelai berdasarkan pola pita DNA hasil RAPD. DNA diekstrak dari daun muda dengan metode CTAB. Penentuan kuantitas dan kualitas DNA hasil pengekstrakan dilakukan dengan elektroforesis pada gel agarose. Hasil menunjukkan bahwa RAPD dengan primer OPA 244 konsentrasi primer 60 pmol dan 2 μl DNA template hasil pengenceran 10 kali dapat membedakan 50 genotip kedelai. Berdasarkan ketidakmiripan 15% dari persamaan Sneath and Sokal simple matching coeffisient (1973), kekerabatan 50 genotip kedelai terbagi menjadi sembilan kelompok. Kata kunci: kedelai, RAPD, keragaman genetik ABSTRACT Information of genetic diversity on soybean germplasms is essential in breeding program. The easiest way for studying genetic diversity is by examining morphological characters. However, some morphological characters are influenced by environmental factors. In contrast, studying genetic diversity by molecular evaluation, i.e. based on DNA markers, gives better result, is more consistent, and is not influenced by environmental factors or growth phases. RAPD (Random Amplified Polymorphic DNA) is one of DNA markers, that can be used for analysing genetic diversity and genetic relationship. This research aimed for studying genetic diversity and genetic relationship among 50 soybean genotypes based on the DNA pattern of RAPD. DNA was extracted from young leaves with CTAB method. The quantity and quality of extracted DNA was determined by agarose gel electrophoresis. Result showed that RAPD with 60 pmol of primer OPA 244 and 2 μl of diluted DNA could be used for differentiating 50 soybean genotypes tested. Based on 15% dissimilarity from Sneath and Sokal simple matching coefficient (1973) 50 soybean genotypes tested could be classified into nine groups. Key words : soybean, RAPD, genetic diversity
Food Insecurity and Obesity Incidence Across Connecticut

Rebecca Boehm, PhD, is an economist with the Food and Environment Program at Union of Concerned Scientists in Washington D.C., and was previously a Postdoctoral Fellow with the Zwick and Rudd Centers at the University of Connecticut. Jaime Foster, PhD, is Senior Director of Community Partnership and Programs at the Connecticut Food Bank and formerly a Postdoctoral Fellow with the Rudd Center. Jiff Martin is Associate Extension Educator for UConn Extension. Rigoberto Lopez, PhD, is the Director of Zwick Center and professor and head of the Department of Agricultural and Resource Economics at UConn.
Insect development : morphogenesis, molting and metamorphosis

The publication of the extensive 7-volume work "Comprehensive Molecular Insect Science" has provided library customers and their end-users with a complete reference encompassing important developments and achievements in modern insect science including reviews on the ecdysone receptor, lipocalins, and bacterial toxins. This derivative from the major reference work, "Insect Development: Metamorphosis, Molting and Morphogenesis", presents a new opportunity for the end user who desires to purchase a comprehensive yet affordable work on these important aspects of insect development. Timeless articles by a host of respected contributors in the field cover such topics as embryonic development, hormonal control of form and function of the nervous system, programmed cell death, organization of the endocrine system, and much more. The articles are specially selected by the known and respected editor-in-chief of the original major reference work. Classic reviews offer essential coverage of development as it relates to metamorphosis, molting and morphogenesis. An introduction by the editor puts the selected body of work in context, highlighting the need for entomologists, developmental biologists and related researchers to have these valuable reviews in their personal collection.
Contributions aux algorithmes stochastiques pour le Big Data et à la théorie des valeurs extrèmes multivariés.

La these comporte deux parties distinctes. La premiere partie concerne des modeles pour les extremes multivaries.On donne une construction de vecteurs aleatoires multivaries a variations regulieres. La construction se base sur une extension multivariee d'un lemme de Breiman etablissant la propriete de variation reguliere d'un produit $RZ$ de variable aleatoire avec $R$ positive a variation reguliere et $Z$ positive suffisamment integrable. En prenant $mathbf{Z}$ multivarie et suffisamment integrable, on montre que $Rmathbf{Z}$ est un vecteur aleatoire a variations regulieres et on caracterise sa mesure limite. On montre ensuite que pour $mathbf{Z}$ de loi bien choisie, on retrouve des modeles stables classiques comme le modele t-extremal, Husler-Reiss, etc. Puis, on etend notre construction pour considerer la notion de variation reguliere multivariee non standard. On montre ensuite que le modele de Pareto (qu'on appelle Husler-Reiss Pareto) associe au modele max-stable Husler-Reiss forme une famille exponentielle complete. On donne quelques proprietes du modele Husler-Reiss Pareto puis on propose un algorithme de simulation exacte. On etudie l'inference par le maximum de vraisemblance. Finalement, on considere une extension du modele Husler-Reiss Pareto utilisant la notion de variation reguliere non standard. On etudie l'inference par le maximum de vraisemblance du modele generalise et on propose une methode d'estimation des parametres. On donne une etude numerique sur l'estimateur du maximum de vraisemblance pour le modele Husler-Reiss Pareto. Dans la second partie qui concerne l'apprentissage statistique, on commence par donner une borne sur la valeur singuliere minimale d'une matrice perturbee par l'ajout d'une colonne. On propose alors un algorithme de selection de colonne afin d'extraire les caracteristiques de la matrice. On illustre notre algorithme sur des donnees reelles de series temporelles ou chaque serie est pris comme etant une colonne de la matrice. Deuxiemement, on montre que si une matrice $X$ a une propriete d'incoherence alors $X$ possede aussi une version affaiblie de la propriete NSP (null space property). Puis, on s'interesse au probleme de selection de matrice incoherente. A partir d'une matrice $Xin mathbb{R}^{n imes p}$ et $mu>0$, on cherche la plus grande sous-matrice de $X$ avec une coherence inferieure a $mu$. Ce probleme est formule comme un programme lineaire avec contrainte quadratique sur ${0,1}^p$. Comme ce probleme est NP-dur, on considere une relaxation sur la sphere et on obtient une borne sur l'erreur lorsqu'on considere le probleme relaxe. Enfin, on analyse l'algorithme de gradient stochastique projete pour l'analyse en composante principale online. On montre qu'en esperance, l'algorithme converge vers un vecteur propre maximum et on propose un algorithme pour selectionner le pas de l'algorithme. On illustre ensuite cet algorithme par une experience de simulation.
The role of episodic simulation in motivating commonplace harms

Every day, people make decisions about whether to harm others, and understanding these decisions is a major aim of social psychology. Here, we show that episodic simulation – a key psychological process implicated in other types of social and moral decision-making – can play a surprising role. Across seven experiments, we find that imagining harming others makes people report a higher likelihood of actually performing that harm in the future, and that this effect happens in part because, when people construe the harm as morally justified (which they tend to do spontaneously), imagining doing it makes them feel good. These findings stand in contrast to traditional accounts of harm aversion in moral psychology, and instead contribute to a growing body of evidence that people often cast harming others in a positive light.
Understanding in vivo degradation of mesoporous silica therapeutic vectors through in situ ellipsometry

Dans les dernieres 15 ans, la recherche biomedicale a explore en profondeur l’utilisation de nanoparticules pour la delivrance ciblee de medicaments. Parmi plusieurs materiaux etudies, la silice mesoporeuse represente une plateforme exceptionnelle pour ce type d’applications puisque elle est biocompatible et capable d’etre charge avec une quantite elevee de medicament, tout en etant facile a synthetiser et a fonctionnaliser . La connaissance des interactions entre nanoparticules de silice et environnement biologique est necessaire pour concevoir des vecteurs therapeutiques efficaces et pas toxiques. Cet etude a developpe une nouvelle methode d’analyse in situ pour suivre les interactions entre silice mesoporeuse et fluides biologiques reels (serum et sang), employant une cellule d’analyse microfluidique et l’ellipsometrie en reflexion totale interne. Nous avons ainsi realise le suivi dynamique de la degradation de vecteurs models a base de silice poreuse structure dans une solution tampon a pH physiologique et une solution concentre de proteines. Ces analyses ont permis d’evaluer l’influence de la structure poreuse, de l’adsorption de proteines sur la surface et de la vitesse du flux sur la dissolution de la silice mesoporeuse.
Researches regarding leptin's influence on the fat and protein percent in buffalo milk.

Leptin is a 16 kDa protein, expressed mainly by the adipose tissue, which plays a crucial role in the regulation of energy expenditure, food intake, and adiposity. Studies made in this field indicate that this hormone can also influence milk’s quality at the following species: bovine, ovine and caprine. No studies have been made concerning this aspect at buffalo milk. Taking into consideration the fact that one of the main characteristics at buffalo’s milk is the high content of fat and protein, this paper focuses on these two compositional parameters and the possible influence that this hormone might have on their percentages in milk. Using the PCR technique we managed to identify the favorable allele C and T of leptin gene responsible for these production traits and afterwards we evaluated the fat and protein percent. Comparing the results we concluded the fact that according to the buffalo’s genotypes CC and TT of leptin gene found at the individuals studied these two parameters vary. Leptin is a proteic hormone produced in the adipose tissue which inhibits the feed intake (
Lakes as food sources for bats : evidence from stable isotopes and acoustic monitoring

Many bats use aquatic habitats to feed and standing water to drink. Given that interactions between aquatic and terrestrial systems are important for understanding food web dynamics and for conserving species and ecosystems, this review examines the data available on bats’ use of aquatic habitats. One of the principal objectives was to assess how eutrophication and other anthropogenic impacts on water bodies affect bats. Most studies have been conducted in Europe and North America. They show, directly or indirectly, how bats use aquatic resources. Acoustic monitoring is the most common technique employed, although some studies have used radiotelemetry or other methods. Myotis daubentonii is the most commonly studied species. Research on this topic does not tend to focus more on threatened species (i.e., those included on the IUCN red list). I conclude that the effects of water pollution and eutrophication on bats remain unclear because different effects are reported for different species and different areas. Furthermore, more studies are needed from Africa, South America, and Asia, regions for which few data are available, as well as from arid regions where standing water is a limited resource. Chapter I Review: bats’ use of aquatic habitats
The Effects of Re-Framing and Respondent Personality on Psychometric Outcomes: A Replication and Extension Study

ii CHAPTER ONE: INTRODUCTION . 1 CHAPTER TWO: METHOD . 14 CHAPTER THREE: RESULTS . 27 CHAPTER FOUR: DISCUSSION . 41 FIGURES AND TABLES . 51 REFERENCES. 63 APPENDIX A . 70 APPENDIX B . 71 APPENDIX C . 72
Bioprospecting of snake venoms: A mini review

Venom is a source of numerous active natural substances that have specific effects on various biological activities. Snake venom contributed significantly to the treatment of many medical conditions and enhancing the knowledge in pharmacology. Information on the nature and mechanism of action of these toxins has enabled a more scientific approach to the treatment of their intoxications. This paper focuses on knowledge about the biochemical and pharmacological importance of sea shake. In addition, this mini-review discusses the shake venoms, toxicity and therapeutic uses of snake venoms.
Diversity of Seasonal Adaptations in Terrestrial True Bugs (Heteroptera) from the Temperate Zone

Heteropterans occupy a wide range of habitats characterized by pronounced seasonality of environmental conditions. In the temperate zone, they use different seasonal adaptations to survive and successfully utilize resources under these sometimes severe circumstances. To review the known adaptations, we use two approaches. First (as suggested by Tanber et al., 1986), all seasonal adaptations are divided into three categories (dormancy, seasonal migration and seasonal polyphenism) with further subdivision of each category into diapause-mediated and non-diapause responses. We suggest here an alternative approach based on the four seasons of the yearly cycle when these adaptations either used by insects or at survival of which these adaptations are aimed to. In the group of winter adaptations, we discuss basic traits of winter diapause (metabolic parameters, facultative and obligatory nature, photoperiodic induction, sensitive stages, temperature dependence, geographical variation of photoperiodic response, use of host plant status and temperature as alternative cues for diapause induction, cold-hardiness etc.). The spring complex of adaptations has been less studied and includes different types of post-diapause photoperiodic sensitivity that are evidently adaptive under different circumstances. As summer and autumn adaptations, we discuss summer diapause, photoperiodic control of nymphal development and coloration of nymphs and adults, behavioral adaptations and migration. Some general considerations and directions for further research are also given.