Miracle-dz/UniFAR-FTUs huggingface dataset:
Interventions for musculoskeletal disorders in computer-intense office work: A framework for evaluation

Workplace interventions for work-related musculoskeletal disorders (WMSDs) are usually multifaceted. These interventions tend to deal with multiple work organizational and physical risk factors and have components occurring at different organizational levels. The organizations are often changing, with shifting initiatives and priorities. Evaluation of such interventions poses challenges in documentation of implementation, reduction in exposures, and assessment of improved health outcomes. We describe a means-outcomes framework for evaluating field interventions that includes strategies, activities, objectives and metrics for outcomes using quantitative and qualitative methods. We demonstrate application of the framework to our work with a large newspaper, which builds on existing laboratory, aetiological and best practice evidence to improve musculoskeletal health. The newspaper had adopted several organizational strategies aimed at improving financial performance, including restructuring into teams, a major set of moves/renovations and introduction of new software. Concomitant WMSD-related organizational strategies include an ergonomics policy, human resources activities, promotion of team work, changes in environment and equipment specifications and development of information systems. We have found the framework useful for focusing the purpose of data collection and ensuring coverage of important components. At the same time, it provides sufficient flexibility to respond to the changing process of implementation.
Glioma Risks Associate With Genetic Polymorphisms of XRCC1 Gene in Chinese Population

Glioma is the most common type of primary brain tumors in adults. Previous evidence indicates that the X‐ray repair cross‐complementing group 1 gene (XRCC1) is an important candidate gene which influencing the pathogenesis of glioma. This study aims to assess the potential associations between glioma risks and genetic polymorphisms of XRCC1 gene. A total of 1,286 Chinese Han ethnic subjects consisting of 638 glioma patients and 648 controls were recruited in this case‐control study. The genotyping of XRCC1 genetic polymorphisms (c.482C>T, c.1161G>A, and c.1804C>A) were conducted using the polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP), created restriction site‐PCR (CRS‐PCR) and DNA sequencing methods. Our data indicated that the allelic and genotypic frequencies of these genetic polymorphisms in glioma patients were significantly different from those of controls. We detected that the alleles/genotypes were statistically associated with the increased risks of glioma (for c.482C>T, TT versus (vs.) CC: OR = 2.24, 95% CI = 1.48–3.39, P < 0.001; T vs. C: OR = 1.30, 95% CI = 1.09–1.53, P = 0.003; for c.1161G>A, AA vs. GG: OR = 1.62, 95% CI = 1.11–2.35, P = 0.012; A vs. G: OR = 1.19, 95% CI = 1.01–1.41, P = 0.040; for c.1804C>A, AA vs. CC: OR = 2.12, 95% CI = 1.45–3.11, P < 0.001; A vs. C: OR = 1.32, 95% CI = 1.12–1.56, P = 0.001). Our findings suggest that these genetic polymorphisms of XRCC1 gene may influence glioma risks in Chinese Han ethnic subjects, and might be potential molecular markers for evaluating glioma risks. J. Cell. Biochem. 115: 1122–1127, 2014. © 2013 Wiley Periodicals, Inc.
Identification and Characterization of MicroRNAs in the Spleen of Common Carp Immune Organ

MicroRNAs (miRNAs) play an important role in the regulation of many fundamental biological processes in eukaryotes; however, miRNAs associated with immune functions in the common carp have not been reported. In this study, a small‐RNA cDNA library was constructed from the spleen of the common carp. A total of 10,603,456 high‐quality clean reads, representing 293,603 unique sequences, were obtained from the small‐cDNA library using the Solexa sequencing. By the bioinformatic analysis, 194 conserved miRNAs and 12 novel miRNAs were identified in the carp spleen. The abundant miRNAs principally belong to 30 miRNA gene families such as let‐7, mir‐10, mir‐15, mir‐30, and so on. The conservation analysis showed that 23 families were present both in protostomes and deuterostomes, 46 families were conserved only in vertebrates, and 5 families (mir‐430, mir‐722, mir‐724, mir‐734, and mir‐738) were identified only in fish species. Furthermore, GO enrichment analysis and KEGG pathway analysis suggested that miRNAs expressed in the spleen of common carp are involved in immune system development, lymphoid organ development, lymphocyte activation, immune response, B cell receptor signaling pathway, T cell receptor signaling pathway, Fc gamma R‐mediated phagocytosis, Toll‐like receptor signaling pathway, and so on. This study described the miRNA transcriptome in spleen tissue for the first time in the common carp. The results expand the number of known common carp miRNAs and provides a meaningful framework to understand the common carp immune system and defense mechanisms. J. Cell. Biochem. 115: 1768–1778, 2014. © 2014 Wiley Periodicals, Inc.
Ultrasound breast tumor segmentation based on 3-D level set

In this paper, we propose a new model based on C-V model, this model based on intra-region similar and inter-region dissimilar properties, adjusting the parameters automatically using the region mean values inside and outside the curve, is suitable for weak edge image segmentation. In the segmentation of the 3-D ultrasonic breast tumor, the segmentation speed of this model is faster than the C-V model, and the segmentation result is gratifying.
Baillière’s Clinical Gastroenterology

Bailliere’s Clinical Gastroenterology. Volume 10. Number 4. Gastrointestinal Endocrine Tumours. O’Shea D, Bloom SR. (Pp 211; illustrated: £30.00.) London: Bailliere Tindall, 1996. ISBN 0-7020-2186-5. This book consists of a series of chapters on each of the major gut endocrine tumour syndromes and four general chapters on the pathology, the peptide receptors possessed by these tumours, and medical and surgical treatment. The emphasis of the book is clinical, and all of the chapters are written by authors who have a large personal experience of these rare tumours. A chapter on carcinoid tumours discusses the characteristics of the various different tumours that arise from the different sites in the gastrointestinal tract. The carcinoid syndrome is briefly discussed, as is prognosis. In this chapter there is virtually no discussion …
SRC Family Kinase Inhibition Through a New Pyrazolo[3,4‐d]Pyrimidine Derivative as a Feasible Approach for Glioblastoma Treatment

Glioblastoma (GB) is the most common and aggressive primary tumor of the central nervous system. The current standard of care for GB consists of surgical resection, followed by radiotherapy combined with temozolomide chemotherapy. However, despite this intensive treatment, the prognosis remains extremely poor. Therefore, more effective therapies are urgently required. Recent studies indicate that SRC family kinases (SFKs) could represent promising molecular targets for GB therapy. Here, we challenged four GB cell lines with a new selective pyrazolo[3,4‐d]pyrimidine derivative SFK inhibitor, called SI221. This compound exerted a significant cytotoxic effect on GB cells, without significantly affecting non‐tumor cells (primary human skin fibroblasts), as evaluated by MTS assay. We also observed that SI221 was more effective than the well‐known SFK inhibitor PP2 in GB cells. Notably, despite the high intrinsic resistance to apoptosis of GB cells, SI221 was able to induce this cell death process in all the GB cell lines, as observed through cytofluorimetric analysis and caspase‐3 assay. SI221 also exerted a long‐term inhibition of GB cell growth and was able to reduce GB cell migration, as shown by clonogenic assay and scratch test, respectively. Moreover, through in vitro pharmacokinetic assays, SI221 proved to have a high metabolic stability and a good potential to cross the blood brain barrier, which is an essential requirement for a drug intended to treat brain tumors. Therefore, despite the need of developing strategies to improve SI221 solubility, our results suggest a potential application of this selective SFK inhibitor in GB therapy. J. Cell. Biochem. 116: 856–863, 2015. © 2014 Wiley Periodicals, Inc.
Targeting Cytokines, Chemokines and Adhesion Molecules in Rheumatoid Arthritis

The therapy of rheumatoid arthritis (RA) was revolutionized by basic research studies and biopharmaceutical development of the first generation of anti-rheumatic biologic agents (i.e. biological response modifiers), which targeted the pro-inflammatory cytokines, tumor necrosis factor-� (TNF-� ), interleukin-1 (IL-1) and interleukin-6 (IL-6). Further studies resulted in biological response modifiers that have also targeted activated T- and B-lymphocytes both of which play a prominent role in RA pathogenesis and disease progression. Despite the relatively impressive RA disease- modifying effects of these biologic agents during the post-FDA approval period, there are reports of moderate to severe adverse events associated with their continuous use. Additionally, there is accumulating evidence indicating that anti- rheumatic biologic agents, while dampening RA disease activity as measured by the American College of Rheumatology (ACR) criteria in clinical trials or other arthritis disease activity instruments employed in studies after approval of these anti-rheumatic medicines by the FDA do not entirely halt the erosive joint cartilage and bone destruction characteristic of RA. There is also still some uncertainty as to how long the available anti-rheumatic biologics can be continuously employed as RA therapies. Since the development of TNF-� /IL-1/IL-6 anti-rheumatic biologics, several additional cytokines with pro-inflammatory activity as well as their receptors, which initiate upstream pathophysiologic effects, have been identified and characterized. In several cases, these cytokines have been cloned and sequenced. Thus, cytokines, such as IL-7, IL12/IL-23, IL-15, IL-16, IL-17/IL-18, IL-19/IL-20/IL-22 and IL-32, have been implicated in arthritis development in animal models of RA and measurements in synovial tissue and synovial fluid obtained from patients with RA also suggested a role for them in RA disease progression, if not in its pathogenesis. There has also been compelling evidence that cytokines with chemokine-activity and adhesion molecules also play a crucial role in RA via their capacity to recruit and retain leukocytes, macrophages, monocytes and endothelial-cell complexes in RA synovial joints. Taken together, non-TNF-� /non-IL-1/non-IL-6 cytokines, chemokines and their receptors as well as adhesion molecules appear to be worthwhile targets for drug development in RA.
EFFECTS OF NITROUS OXIDE ON THE RESPIRATORY PATTERN OF SPONTANEOUSLY BREATHING CHILDREN DURING ANAESTHESIA A computerized study

The effects of the withdrawal of nitrous oxide from the inspired gas mixture were studied in 10 spontaneously breathing children during nitrous oxide-halothane anaesthesia, before and during surgery, using a computerized system for the measurement, recording and analysis of data. Before surgery the decline in the alveolar nitrous oxide concentration was associated with an increase in minute ventilation (32.7%, P
miR‐155 Regulates Glioma Cells Invasion and Chemosensitivity by p38 Isforms In Vitro

The critical role of microRNAs in cancer development has been extensively described. miRNAs are both specific markers and putative therapy targets. miR‐155 has been identified to be an oncomiRNA and is highly expressed in several solid cancers, including glioblastoma. In this study, we found that miR‐155 is a good potential therapy target. Knockdown of miR‐155 sensitizes glioma cells to the chemotherapy of temozolomide (TMZ) by targeting the p38 isoforms mitogen‐activated protein kinase 13 [MAPK13, also known as p38 MAPKδ or stress‐activated protein kinase 4 (SAPK4)] and MAPK14 (also known as p38 MAPKα). As tumor suppressor genes, MAPK13 and MAPK14 play important roles in lowering the accumulation of reactive oxygen species (ROS), inducing cell apoptosis, and slowing the progression of cancer. Knockdown of miR‐155 enhanced the anticancer effect of TMZ on glioma by targeting the MAPK13 and MAPK14‐mediated oxidative stress and apoptosis, but did not affect the secretion of MMP2 and MMP9. J. Cell. Biochem. 116: 1213–1221, 2015. © 2014 Wiley Periodicals, Inc.
Vitamin D Control of Hematopoietic Cell Differentiation and Leukemia

It is now well known that in the mammalian body vitamin D is converted by successive hydroxylations to 1,25‐dihydroxyvitamin D (1,25D), a steroid‐like hormone with pleiotropic properties. These include important contributions to the control of cell proliferation, survival and differentiation, as well as the regulation of immune responses in disease. Here, we present recent advances in current understanding of the role of 1,25D in myelopoiesis and lymphopoiesis, and the potential of 1,25D and analogs (vitamin D derivatives; VDDs) for the control of hematopoietic malignancies. The reasons for the unimpressive results of most clinical studies of the therapeutic effects of VDDs in leukemia and related diseases may include the lack of a precise rationale for the conduct of these studies. Further, clinical trials to date have generally used extremely heterogeneous patient populations and, in many cases, small numbers of patients, generally without controls. Although low calcemic VDDs have been used and combined with agents that can increase the leukemia cell killing or differentiation effects in acute leukemias, the sequencing of agents used for combination therapy should to be more clearly delineated. Most importantly, it is recommended that in future clinical trials the rationale for the basis of the enhancing action of drug combinations should be clearly articulated and the effects on anticancer immunity should also be evaluated. J. Cell. Biochem. 116: 1500–1512, 2015. © 2015 Wiley Periodicals, Inc.
The Key Regulator for Language and Speech Development, FOXP2, is a Novel Substrate for SUMOylation

Transcription factor forkhead box protein P2 (FOXP2) plays an essential role in the development of language and speech. However, the transcriptional activity of FOXP2 regulated by the post‐translational modifications remains unknown. Here, we demonstrated that FOXP2 is clearly defined as a SUMO target protein at the cellular levels as FOXP2 is covalently modified by both SUMO1 and SUMO3. Furthermore, SUMOylation of FOXP2 was significantly decreased by SENP2 (a specific SUMOylation protease). We further showed that FOXP2 is selectively SUMOylated in vivo on a phylogenetically conserved lysine 674 but the SUMOylation does not alter subcellular localization and stability of FOXP2. Interestingly, we observed that human etiological FOXP2 R553H mutation robustly reduces its SUMOylation potential as compared to wild‐type FOXP2. In addition, the acidic residues downstream the core SUMO motif on FOXP2 are required for its full SUMOylation capacity. Finally, our functional analysis using reporter gene assays showed that SUMOylation may modulate transcriptional activity of FOXP2 in regulating downstream target genes (DISC1, SRPX2, and MiR200c). Altogether, we provide the first evidence that FOXP2 is a substrate for SUMOylation and SUMOylation of FOXP2 plays a functional role in regulating its transcriptional activity. J. Cell. Biochem. 117: 426–438, 2016. © 2015 Wiley Periodicals, Inc.
FOXD3 Promotes PAX3 Expression in Melanoma Cells

Several key transcription factors regulate cell growth, survival, and differentiation during neural crest and melanoblast development in the embryo, and these same pathways may be reactivated in tumors arising from the progenitors of these cells. The transcription factors PAX3 and FOXD3 have essential roles in melanoblasts and melanoma. In this study, we define a regulatory pathway where FOXD3 promotes the expression of PAX3. Both factors are expressed in melanoma cells and there is a positive correlation between the transcript levels of PAX3 and FOXD3. The PAX3 gene contains two FOX binding motifs within highly conserved enhancer regulatory elements that are essential for neural crest development. FOXD3 binds to both of these motifs in vitro but only one of these sites is preferentially utilized in melanoma cells. Overexpression of FOXD3 upregulates PAX3 levels while inhibition of FOXD3 function does not alter PAX3 protein levels, supporting that FOXD3 is sufficient but not necessary to drive PAX3 expression in melanoma cells. Here, we identify a molecular pathway where FOXD3 upregulates PAX3 expression and therefore contributes to melanoma progression. J. Cell. Biochem. 117: 533–541, 2016. © 2015 Wiley Periodicals, Inc.
Viscoelastic properties of attractive and repulsive colloidal glasses

We report a numerical study of the shear viscosity and the frequency dependent elastic moduli close to dynamical arrest for a model of short range attractive colloids, both for the repulsive and the attractive glass transition. Calculating the stress autocorrelation functions, we find that density fluctuations of wavevectors close to the first peak in the structure factor control the viscosity rise on approaching the repulsive glass, while fluctuations of larger wavevectors control the viscosity close to the attractive glass. On approaching the glass transition, the viscosity diverges with a power law with the same exponent as the density autocorrelation time.
One-dimensional optical thermal ratchets

The ability to rectify Brownian forces with spatially extended time-varying light fields creates new opportunities for studying the statistical properties of thermal ratchet models and to exploit these models’ interesting and useful properties for practical applications. This paper describes experimental studies of one-dimensional thermal ratchets implemented with the holographic optical trapping technique applied to fluid-borne colloidal spheres. These studies demonstrate the complementary roles of global spatiotemporal symmetry and local dynamics in establishing the direction of ratchet-induced motion and also highlight avenues for future advances in higher-dimensional systems.
Elevated Expression of Hepatoma Up‐Regulated Protein Inhibits γ‐Irradiation‐Induced Apoptosis of Prostate Cancer Cells

Despite progression in diagnosis and treatment, prostate cancer (PCa) still represents the main cause of cancer‐related mortality and morbidity in men. Although radiation therapy offers clinical benefit over other therapeutic modalities, the success of this therapeutic modality is commonly hampered by the resistance of advanced tumors. So far, the mechanisms governing tumor resistance to radiotherapy are not discussed in detail. Here, we demonstrate for the first time that the resistance of PCa to radiation therapy is attributed to elevated expression of Hepatoma Up‐Regulated Protein (HURP). In PCa cells, the induction of HURP expression suppresses γ‐irradiation‐induced apoptosis. γ‐irradiation‐induced apoptosis of PCa cells is associated with expression of E2F1, p53, p21 proteins together with the phosphorylation of apoptosis signal‐regulating kinase1 (ASK1), c‐jun‐N‐terminal kinase (JNK) and Ataxia‐telangiectasia mutated (ATM) and histone family member X (H2AX). Whereas, the induction of HURP expression is able to suppress γ‐irradiation‐induced effects on E2F1, p53, p21, ATM, ASK1, JNK and ATM, and H2AX. Also, inhibition of γ‐irradiation‐induced‐ cytochrome c release, cleavage of caspase‐9, caspase‐3, PARP, and reactive oxygen species (ROS) were noted in PCa cells induced for HURP expression. The observed radio‐resistance of PCa is thought to be the consequence of HURP‐mediated destabilization of p53 and ATM proteins that are essential for the modulation of γ‐irradiation‐induced apoptosis. Thus, based on our findings, PCa resistance to radiation therapy results from the deregulation of ASK1/ JNK; ATM/ H2AX; ATM/p53 and checkpoint kinase 2 (Chk2)/ E2F‐1 in response to the elevated expression of HURP. J. Cell. Biochem. 117: 1308–1318, 2016. © 2015 Wiley Periodicals, Inc.
Myocardial Response to Pretreatment with L-Carnitine in Patients with Cardiac Valve Replacement

Twenty-one patients undergoing mitral valve replacement (MVR) with either isolated or combined valvular procedure were investigated to determine the ef fect of randomized and prospective administration of L-carnitine (car) chloride on myocardial function. Carnitine chloride (1800 mg/day, for seven consecu tive days just prior to MVR) was given orally to 9 patients (car-treated group) who were compared with 12 car-untreated patients (control group) subjected to the same procedure. Valve replacement was performed using the Bjork-Shiley mechanical prosthesis in aortic and mitral position and bioprosthesis in tricuspid position in both groups under cardiopulmonary bypass (CPB) associated with cold potassium-magnesium cardioplegia and systemic hypothermia. Preopera tive and intraoperative clinical profile (age, functional class, duration of aortic clamp and CPB, and level of hypothermia) was well matched in both groups. Free, short-chain acyl, long-chain acyl, and total Car were measured in the papil lary muscle excised adjacent to the free wall of the left ventricle at the time of valvular surgery. Heart rate and mean arterial pressure did not differ between the two groups. Cardiac index was 3.44 ± 0.08 and 3.82 ± 0.13 L/m2/min in the control and car-treated groups, respectively, (p < 0.05). Dobutamine (DOB) infusion rate was 8.55 ± 0.73 and 5.57 ± 0.95 μg/kg/min in the control and car-treated groups, respectively (p < 0.05). Left ventricular stroke work index (LVSWI)/DOB ratio was 4.50 ± 0.65 and 8.16 ± 1.72 g*m/m 2/beat/unit of DOB, in the control and car-treated groups, respectively (p < 0.05). Long-chain acyl carnitine was 0.224 ± 0.031 and 0.460 ± 0.096 nmoL/mg noncollagenous protein in the control and the car-treated groups, respectively (p<0.05). LVSWI/DOB ratio was well correlated with long-chain acyl carnitine in the tis sue, r = 0.74, n = 18, p<0.001. These findings suggest that pretreatment with L-carnitine chloride appears to be effective in improving myocardial function in the early phase of reperfu sion following surgery and that long-chain acyl carnitine may play a key role in the enhancement of carnitine-meditated mitochondrial metabolic process.
Care and Feeding of the Immune System

The immune system does much more than help you recover from a cold or flu. It locates and tries to digest anything, living or inert, that's identified as not being you. In short, its job description is to connect us with the world while keeping us safe and distinct. Clients often do not understand the importance of their immune system, how it works, or how to nurture it and keep it strong. This article describes a variety of approaches, including imagery, Ayurveda, and nutrition, that you can teach your clients, so that they can better care for and feed their immune systems.