Miracle-dz/UniFAR-FTUs huggingface dataset:
Exogenous arachidonic acid inactivates protein kinase C in mouse pancreatic islets.

The effect of arachidonic acid on protein kinase C activity and insulin secretion in mouse islets was investigated. Arachidonic acid stimulated protein kinase C activity in islet cytosol and membrane fractions by substituting for phosphatidylserine. Stimulation by arachidonic acid was dependent on either Ca2+ or the phorbol ester 12-O-tetradecanoylphorbol 13-acetate, was potentiated by the combined addition of Ca(2+) + 12-O-tetradecanoylphorbol 13-acetate, and did not further increase protein kinase C activity in the presence of saturating concentrations of phosphatidylserine. Arachidonic acid stimulation of protein kinase C was prevented by binding of arachidonic acid to albumin. In the absence of extracellular Ca2+, exogenous arachidonic acid stimulated insulin secretion. Arachidonic acid-induced insulin secretion was not potentiated by 12-O-tetradecanoylphorbol 13-acetate and was not prevented by the protein kinase C inhibitor staurosporine, suggesting that arachidonic acid-induced insulin secretion may occur independently of protein kinase C activation. Arachidonic acid-induced insulin secretion in Ca(2+)-free medium was on the other hand potentiated by addition of extracellular Ca2+. Stimulation of insulin secretion by exogenous arachidonic acid was associated with inactivation of protein kinase C. Inactivation of protein kinase C was also observed in islet homogenate after pre-incubation with arachidonic acid. Arachidonic acid-induced protein kinase C inactivation in islet homogenate was prevented by albumin or MgATP. Inactivation by arachidonic acid in intact islets was, however, not produced during enzyme isolation and was not prevented by inclusion of albumin or MgATP during preparation of protein kinase C extracts. (ABSTRACT TRUNCATED AT 250 WORDS)
External adjustment sensitivity analysis for unmeasured confounding: an application to coronary stent outcomes, Pennsylvania 2004-2008.

BACKGROUND Assessing the real-world comparative effectiveness of common interventions is challenged by unmeasured confounding. OBJECTIVE To determine whether the mortality benefit shown for drug-eluting stents (DES) over bare metal stents (BMS) in observational studies persists after controls for/tests for confounding. DATA SOURCES/STUDY SETTING Retrospective observational study involving 38,019 patients, 65 years or older admitted for an index percutaneous coronary intervention receiving DES or BMS in Pennsylvania in 2004-2005 followed up for death through 3 years. STUDY DESIGN Analysis was at the patient level. Mortality was analyzed with Cox proportional hazards models allowing for stratification by disease severity or DES use propensity, accounting for clustering of patients. Instrumental variables analysis used lagged physician stent usage to proxy for the focal stent type decision. A method originating in work by Cornfield and others in 1954 and popularized by Greenland in 1996 was used to assess robustness to confounding. PRINCIPAL FINDINGS DES was associated with a significantly lower adjusted risk of death at 3 years in Cox and in instrumented analyses. An implausibly strong hypothetical unobserved confounder would be required to fully explain these results. CONCLUSIONS Confounding by indication can bias observational studies. No strong evidence of such selection biases was found in the reduced risk of death among elderly patients receiving DES instead of BMS in a Pennsylvanian state-wide population.
Crowdsourcing business models that last

Purpose The purpose of this paper is to discuss how to sustain crowdsourcing business models. Emerging companies are innovating their business model to rely on a crowd of participants and involve contributing users in value capture. While some organizations demonstrate initial success, sustaining a crowdsourcing business model is challenging. Design/methodology/approach The study is based on a comparative case study of the prominent crowdsourcing communities Threadless and Quirky. Participatory observation resulted in over 380 analyzed comments. Findings Seven lessons from Threadless’s success and Quirky’s failure are discussed to derive implications for sustaining crowdsourcing business models. Research limitations/implications Because both cases are integrator platforms build around contests, other crowdsourcing platform types should be studied to enrich the findings. Practical implications Managers receive guidance on how to design a sustainable business model that involves the crowd in creating value and lets the crowd participate in value capture. Originality/value Current research primarily addresses the question of how companies can take advantage of crowdsourcing and mainly considers corporate value capture. The original contribution of this article is a set of strategies to sustain crowdsourcing platforms by taking a platform’s entire business model into account.
‘Optimum Mobility’ Facelift. Part 1 – the Theory

Traditional rhytidectomy techniques, such as the cutaneous lift, the superficial musculoaponeurotic system lift, the deep plane lift and the subperiosteal lift, are mostly differentiated by their different planes of dissection. As well, many of these techniques consider the complete mobilization of tissues a prerequisite for obtaining a satisfactory result. However, is it true that the result of a rhytidectomy is linked to the choice of the dissection plane? Also, is it true that the adequacy of the surgical mobilization of tissues is vital to the outcome? the present paper discusses the above questions and introduces a factor that is believed to be crucial to the planning and success of a rhytidectomy: facial tissue mobility. The analysis of this mobility is presented and leads to the development of three theories: ‘intrinsic mobility’, ‘surgically induced mobility’ and ‘optimum mobility points’. These theories form the foundation of a rhytidectomy technique termed ‘optimum mobility’ facelift.
Which Pairs of Stocks should we Trade? Selection of Pairs for Statistical Arbitrage and Pairs Trading in Karachi Stock Exchange

Pairs Trading refers to a statistical arbitrage approach devised to take advantage from short term fluctuations simultaneously depicted by two stocks from long run equilibrium position. In this study a technique has been designed for the selection of pairs for pairs trading strategy. Engle-Granger 2-step Cointegration approach has been applied for identifying the trading pairs. The data employed in this study comprised of daily stock prices of Commercial Banks and Financial Services Sector. Restricted pairs have been formed out of highly liquid log share price series of 22 Commercial Banks and 19 Financial Services companies listed on Karachi Stock Exchange. Sample time period extended from November 2, 2009 to June 28, 2013 having total 911 observations for each share prices series incorporated in the study. Out of 231 pairs of commercial banks 25 were found cointegrated whereas 40 cointegrated pairs were identified among 156 pairs formed in Financial Services Sector. Furthermore a Cointegration relationship was estimated by regressing one stock price series on another, whereas the order of regression is accessed through Granger Causality Test. The mean reverting residual of Cointegration regression is modeled through the Vector Error Correction Model in order to assess the speed of adjustment coefficient for the statistical arbitrage opportunity. The findings of the study depict that the cointegrated stocks can be combined linearly in a long/short portfolio having stationary dynamics. Although for the given strategy profitability has not been assessed in this study yet the VECM results for residual series show significant deviations around the mean which identify the statistical arbitrage opportunity and ensure profitability of the pairs trading strategy.
Poisonous animal bites in the Israel Defense Forces.

INTRODUCTION Soldiers in field units of the Israel Defense Forces (IDF) are susceptible to injury by various poisonous animals during training and operations. Bites and envenomations by animals such as snakes, scorpions, and spiders can be painful and debilitating, and at times life-threatening. We have examined the extent of exposure of IDF soldiers to snake and arthropod bites and the morbidity resulting from these encounters. METHODS All reports of IDF soldiers who sought medical attention for snake or arthropod bites between the years 1993-1997 were reviewed at the IDF Medical Corps Headquarters. Monthly distribution of cases was noted for all years, and geographic distribution was studied for all 1997 cases. RESULTS Over the period 1993-1997 there was a yearly rate of 32-52 physician visits per 100,000 soldiers due to snakebites (mean 43.6/100,000), and 1370-1729 physician visits per 100,000 soldiers due to arthropod bites (mean 1478/100,000). There is a clear overall increase in snake and arthropod bites during the spring and summer months, with a peak in snakebites in May and in arthropod bites in August. 58% of all snakebites in Israel were reported in the central region, with 33% occurring in the south, and 9% in the north of the country. No fatalities due to envenomations have been reported in the IDF in recent years. CONCLUSION Poisonous animal species pose a significant threat to the soldiers of the IDF. Overall, envenomation is a common and widespread problem that has significant impact on the military medical system, especially during the spring and summer months. It is possible through institution of proper preventive measures to decrease the exposure of IDF personnel to this environmental hazard.
Effect of carnosine on gentamicin-induced nephrotoxicity.

BACKGROUND Gentamicin (GM) is an antibiotic whose clinical use is limited by its nephrotoxicity. Thus the present study was undertaken to investigate if carnosine, an antioxidant, could protect the kidney in this experimental model. MATERIAL/METHODS The animals were divided into seven groups each of 10 animals: one control group, two healthy carnosine groups (10 mg/kg/day), two GM groups (80 mg/kg/day), and two carnosine-GM groups. Kidney function tests, histopathological, ultrastructural, and enzymatic histochemical studies clarified GM nephrotoxicity. RESULTS GM rat showed early kidney function failure as blood creatinine and blood urea were significantly increased after one and two weeks. Experimental evidence suggested a role of reactive oxygen species in GM-induced nephrotoxicity. Histopathological examination revealed degenerative changes in glomeruli and tubules. Ultrastructural study showed glomerular changes, some degeneration of both distal and collecting tubules. The proximal tubules showed marked degrees of changes and necrosis. Enzymatic histochemical studies of GM rats revealed marked elevation of lactate dehydrogenase (LDH) and inhibition of succinic dehydrogenase (SDH), alkaline phosphatases (ALP), acid phosphatases (ACP), and adenosine triphosphatase (ATPase). Blood creatinine and urea were normalized in the carnosine-GM group after one and two weeks. Structural and enzymatic histochemical pictures were greatly ameliorated. CONCLUSIONS The mechanism by which carnosine has a protective effect on GM-induced nephrotoxicity was attributed to its many actions: double antioxidant action, protein molecule protection, removal of harmfully modified ones, activation of immune system, preservation of membrane fluidity, and cytosolic buffering. Carnosine thus offers a promise of ameliorating GM nephrotoxicity.
Anterior-posterior gene expression differences in three Lake Malawi cichlid fishes with variation in body stripe orientation

Morphological differentiation among closely related species provides opportunities to study mechanisms shaping natural phenotypic variation. Here, we address variation in the orientation of melanin-colored body stripes in three cichlid species of the tribe Haplochromini. Melanochromis auratus displays a common pattern of dark, straight horizontal body stripes, whereas in Aristochromis christyi and Buccochromis rhoadesii, oblique stripes extend from the anterior dorsal to the posterior mid-lateral trunk. We first validated a stably reference gene, and then, investigated the chromatophore distribution in the skin by assessing the expression levels of the iridophore and melanophore marker genes, ltk and slc24a5, respectively, as well as pmel, a melanophore pigmentation marker gene. We found anterior-posterior differences in the expression levels of the three genes in the oblique-striped species. The higher anterior expression of ltk, indicates increased iridophore density in the anterior region, i.e., uneven horizontal distribution of iridophores, which coincides with the anterior dorsalization of melanophore stripe in these species. The obliqueness of the horizontal body stripes might be a result of distinct migratory or patterning abilities of melanophores in anterior and posterior stripe regions which could be reflected by variation in the expression of genes involved in melanophore patterning. To address this, we investigated anterior-posterior expression levels of a primary set of candidate target genes with known functions in melanophore migration and stripe patterning in the adult zebrafish, and their related gene regulatory network. Among these genes, those with differences in anterior-posterior expression showed only species-specific differential expression, e.g., sdf1a, col14a1a, ifitm5, and agpat3, with the exception of fbxw4/hagoromo (differentially expressed in an oblique-and the straight-striped species). In summary, distinct anterior-posterior gradients in iridophore density found to be more similar characteristic between the two oblique-striped species. Furthermore, the species-specific differential expression of genes involved in stripe patterning might also implicate distinct molecular processes underlying the obliqueness of body stripe in two closely related cichlid species.
From NIMBY to enlightened resistance: a framework proposal to decrypt land-use disputes based on a landfill opposition case in France

ABSTRACT Not In My Back Yard (NIMBY) supporters are presented as citizens in opposition to local developments due to their spatial proximity. However, these conflicts have proven much more complex than the NIMBY concept can explain. The objective of this paper is to provide a framework to facilitate the understanding of opposition movements and how they can affect society at large, triggering social change. The conceptual framework is applied to a case of local opposition to a landfill project in Essonne, France. Through the analysis of the structure of the opposition movement and its changes over time, the author shows how it can evolve into a social movement that enriches democracy through the constitution of four types of capital: social, scientific, patrimonial and political. The author argues that scientific and patrimonial capitals allow social capital to evolve into political capital. The shift from being a self-interest to a civic interest movement is called enlightened resistance, which reveals local public interest, called territorial interests. When studying environmental controversies, the author emphasises the importance of recognizing the evolution through time of (1) social landscape, (2) different types of legitimate knowledge, (3) the role of place attachment, and (4) the political dimension of identities.
Clearance in smaller airways of inhaled 6-microm particles in subjects with immotile-cilia syndrome.

In subjects with an inherited lack of mucociliary transport, so called immotile-cilia syndrome (ICS), coughing effectively clears particles deposited in larger airways of the tracheobronchial region. The present study investigated clearance in smaller airways of 111In-labeled 6-microm (aerodynamic diameter) monodisperse Teflon particles in six subjects with ICS. The particles were inhaled at an extremely slow flow, 0.05 L/s. Theoretical calculations and experimental data in healthy subjects using this slow flow support particle deposition mainly in smaller ciliated airways, i.e., in bronchioli (generations 12-16). This contrasts with the more centrally deposited pattern obtained using a normal inhalation flow, 0.5 L/s. Lung retention was measured at 0, 24, 48,72 and 96 h. Clearance was significant every 24 h measured over the first 72 h, whereupon it slowed down. The fractions of retained particles were significantly (p < .01) larger than those found for healthy subjects using the slow inhalation flow and those found for ICS subjects using a normal inhalation flow. The results indicate that clearance of particles in smaller airways is incomplete and that cough cannot fully compensate for the lack of mucociliary transport in this region.
Monetary Policy, Private Debt and Financial Stability Risks

Can monetary policy be used to promote financial stability? We answer this question by estimating the impact of a monetary policy shock on private-sector leverage and the likelihood of a financial crisis. Impulse responses obtained from a panel VAR model of 18 advanced countries suggest that the debt-to-GDP ratio rises in the short run following an unexpected tightening in monetary policy. As a consequence, the likelihood of a financial crisis increases, as estimated from a panel logit regression. However, in the long run, output recovers and higher borrowing costs discourage new lending, leading to a deleveraging of the private sector. A lower debt-to-GDP ratio in turn reduces the likelihood of a financial crisis. These results suggest that monetary policy can achieve a less risky financial system in the long run but could fuel financial instability in the short run. We also find that the ultimate effects of a monetary policy tightening on the probability of a financial crisis depend on the leverage of the private sector: the higher the initial value of the debt-to-GDP ratio, the more beneficial the monetary policy intervention in the long run, but the more destabilizing in the short run.
Performance comparison of different ADO-OFDM channel equalization algorithms in visible light communication system

We investigate and analyze the asymmetrically clipped and direct-current-biased optical orthogonal frequency division multiplexing (ADO-OFDM) in visible light communication (VLC) system. Then we propose two modified channel equalization algorithms of ADO-OFDM in VLC systems. It is shown that the algorithm combined with channel estimation for interference cancellation to recover ADO-OFDM signal is more effective in VLC system, which has the best BER performance.
Effect of gallium nitrate on tamoxifen induced hypercalcemia in rats bearing mammary tumor.

AIM To study the effect of gallium nitrate in the treatment of flare hypercalcemia in rats, bearing mammary tumor with bone metastasis. MATERIALS AND METHODS Female Sprague-Dawley albino rats were used in the study. Animals were divided into 5 groups: normal control; hypercalcemic rats bearing DMBA-induced mammary tumors; flare hypercalcemic animals bearing DMBA-induced mammary tumors (hypercalcemic rats, treated with tamoxifen at the dose of 10 mg/kg); flare hypercalcemic rat bearing DMBA-induced mammary tumors, treated with gallium nitrate at the dose of 2.5 mg/kg; control rats, treated with gallium nitrate at the dose of 2.5 mg/kg. Eligibility criteria - serum calcium levels were 11.0 mg% or above. Biochemical parameters were measured, using standard methods. Urinary excretion of calcium, creatinine ratio, urinary bone marker were also evaluated by using standard method. RESULTS All flare hypercalcemic rats were treated with gallium nitrate and developed normocalcemia. Biochemical parameters were measured in hypercalcemic and flare hypercalcemic animals. Calcium level in blood serum, alkaline phosphatase were significantly higher in flare hypercalcemia than in hypercalcemic rats. Urinary pyridinoline, deoxypyridinoline and hydroxyproline were also elevated in flare hypercalcemic rats. In contrast, intact parathyroid hormone and albumin levels were lowered in flare as well as hypercalcemic groups when compared with normal control groups. After gallium nitrate treatment all the above parameters returned to normal level. CONCLUSIONS Administration of gallium nitrate in vivo is highly effective in treatment of flare hypercalcemia.
Rab GTPases regulating receptor trafficking at the late endosome–lysosome membranes

Lysosomes serve key degradative functions for the turnover of membrane lipids and protein components. Its biogenesis is principally dependent on exocytic traffic from the late endosome via the trans‐Golgi network, and it also receives cargo to be degraded from the endocytic pathway. Membrane trafficking to the late endosome–lysosome is tightly regulated to maintain the amplitude of signalling events and cellular homeostasis. Key coordinators of lysosomal traffic include members of the Rab small GTPase family. Amongst these, Rab7, Rab9 and the more recently studied Rab22B/31 have all been reported to regulate membrane trafficking processed at the late endosome–lysosome system. We discuss what is known about the roles of these Rab proteins and their interacting partners on the regulation of traffic of important receptor proteins such as the epidermal growth factor receptor (EGFR) and the mannose 6‐phosphate receptor (M6PR), in association with the late endosome–lysosome system. Better knowledge of EGFR and M6PR traffic in this regard may aid in understanding the pathological processes, such as oncogenic transformations associated with these receptors. Copyright © 2012 John Wiley & Sons, Ltd.
The molecular journey from ductal carcinoma in situ to invasive breast cancer

Ductal carcinoma in situ (DCIS) accounts for approximately 20% of mammographically detected breast cancers. DCIS can recur or progress to invasive breast cancer, but the ability to predict the outcome of patients with DCIS remains limited, leading to inappropriate treatment choices. To the authors' knowledge to date, the hunt for molecular prognostic markers for DCIS has been unsuccessful. Emerging technologies, however, are shedding new light on the biologic course of DCIS. In the current study, the authors review recent findings elucidating the molecular journey from DCIS to invasive cancer and discuss how these findings will lead to more effective treatment with minimization of morbidity. Cancer 2008. © 2008 American Cancer Society.
Blood and bone marrow changes in malaria.

A variety of abnormalities in the number, morphology and function of blood and bone marrow cells may be found in Plasmodium falciparum and P. vivax malaria. In a non-immune individual, the nature of such abnormalities depends on the time after infection. In others it is determined by the pattern and intensity of malaria transmission in the area and the extent of host immunity. Severe anaemia may occur in children with chronic falciparum malaria and low parasitaemia as well as in patients with complicated acute falciparum malaria with high parasitaemia. However, the mechanisms underlying the anaemia in these two situations appear to be different. The possible roles of parasite products, T-cell-derived cytokines produced in response to the infection, macrophage activation and hyperplasia, macrophage-derived factors such as tumour necrosis factor-alpha, and macrophage dysfunction in the pathogenesis of the haematological abnormalities are discussed.