Miracle-dz/UniFAR-FTUs huggingface dataset:
Being a ‘good mother’: Dietary governmentality in the family food practices of three ethnocultural groups in Canada

In this qualitative study with three ethnocultural groups in two regions of Canada, we explore how official dietary guidelines provide particular standards concerning ‘healthy eating’ that marginalize other understandings of the relationship between food and health. In families where parents and youth held shared ways of understanding healthy eating, the role of ‘good mother’ was constructed so as to include healthy eating expertise. Mothers expressed a perceived need to be personally responsible for providing skills and knowledge about healthy eating as well as guarding children against negative nutritional influences. Governing of family eating practices to conform to official nutritional advice occurred through information provision, monitoring in shopping and meal preparation, restricting and guiding food purchases, and directly translating expert knowledges into family food practices. In families where parents and youth held differing understandings of healthy eating, primarily families from ethnocultural minority groups, mothers often did not employ the particular western-originating strategies of conveying healthy eating information, or mentoring healthy meal preparation, nor did they regulate or restrict children’s food consumption. Western dietary guidelines entered into the family primarily through the youth, emphasizing the nutritional properties of food, often devaluing ‘traditional’ knowledge about healthy eating. These processes exemplify techniques of governmentality which simultaneously exercise control over people’s behaviour through normalizing some family food practices and marginalizing others.
Seasonal Diversity of Planktonic Protists in Southwestern Alberta Rivers over a 1-Year Period as Revealed by Terminal Restriction Fragment Length Polymorphism and 18S rRNA Gene Library Analyses

ABSTRACT The temporal dynamics of planktonic protists in river water have received limited attention despite their ecological significance and recent studies linking phagotrophic protists to the persistence of human-pathogenic bacteria. Using molecular-based techniques targeting the 18S rRNA gene, we studied the seasonal diversity of planktonic protists in Southwestern Alberta rivers (Oldman River Basin) over a 1-year period. Nonmetric multidimensional scaling analysis of terminal restriction fragment length polymorphism (T-RFLP) data revealed distinct shifts in protistan community profiles that corresponded to season rather than geographical location. Community structures were examined by using clone library analysis; HaeIII restriction profiles of 18S rRNA gene amplicons were used to remove prevalent solanaceous plant clones prior to sequencing. Sanger sequencing of the V1-to-V3 region of the 18S rRNA gene libraries from spring, summer, fall, and winter supported the T-RFLP results and showed marked seasonal differences in the protistan community structure. The spring library was dominated by Chloroplastidae (29.8%), Centrohelida (28.1%), and Alveolata (25.5%), while the summer and fall libraries contained primarily fungal clones (83.0% and 88.0%, respectively). Alveolata (35.6%), Euglenozoa (24.4%), Chloroplastida (15.6%), and Fungi (15.6%) dominated the winter library. These data demonstrate that planktonic protists, including protozoa, are abundant in river water in Southwestern Alberta and that conspicuous seasonal shifts occur in the community structure.
Noninvasive Fault Classification, Robustness and Recovery Time Measurement in Microprocessor-Type Architectures Subjected to Radiation-Induced Errors

In critical digital designs such as aerospace or safety equipment, radiation-induced upset events (single-event effects or SEEs) can produce adverse effects, and therefore, the ability to compare the sensitivity of various proposed solutions is desirable. As custom-hardened microprocessor solutions can be very costly, the reliability of various commercial off-the-shelf (COTS) processors can be evaluated to see if there is a commercially available microprocessor or microprocessor-type intellectual property (IP) with adequate robustness for the specific application. Most existing approaches for the measurement of this robustness of the microprocessor involve diverting the program flow and timing to introduce the bit flips via interrupts and embedded handlers added to the application program. In this paper, a tool based on an emulation platform using Xilinx field programmable gate arrays (FPGAs) is described, which provides an environment and methodology for the evaluation of the sensitivity of microprocessor architectures, using dynamic runtime fault injection. A case study is presented, where the robustness of MicroBlaze and Leon3 microprocessors executing a simple signal processing task written in C language is evaluated and compared. A hardened version of the program, where the key variables are protected, has also been tested, and its contributions to system robustness have also been evaluated. In addition, this paper presents a further improvement in the developed tool that allows not only the measurement of microprocessor robustness but, in addition, the study and classification of single-event upset (SEU) effects and the exact measurement of the recovery time (the time that the microprocessor takes to self repair and recover the fault-free state). The measurement of this recovery time is important for real-time critical applications, where criticality depends on both data correctness and timing. To demonstrate the proposed improvements, a new software program that implements two different software hardening techniques (one for Data and another for Control Flow) has been made, and a study of the recovery times in some significant fault-injection cases has been performed over the Leon3 processor.
Cellular Responses of the Late Blight Pathogen Phytophthora infestans to Cyclic Lipopeptide Surfactants and Their Dependence on G Proteins

ABSTRACT Oomycete pathogens cause major yield losses for many crop plants, and their control depends heavily on agrochemicals. Cyclic lipopeptides (CLPs) were recently discovered as a new class of natural compounds with strong activities against oomycetes. The CLP massetolide A (Mass A), produced by Pseudomonas fluorescens, has zoosporicidal activity, induces systemic resistance, and reduces late blight in tomato. To gain further insight into the modes of action of CLPs, the effects of Mass A on pore formation, mycelial growth, sporangium formation, and zoospore behavior were investigated, as was the involvement of G proteins in the sensitivity of Phytophthora infestans to Mass A. The results showed that Mass A induced the formation of transmembrane pores with an estimated size of between 1.2 and 1.8 nm. Dose-response experiments revealed that zoospores were the most sensitive to Mass A, followed by mycelium and cysts. Mass A significantly reduced sporangium formation and caused increased branching and swelling of hyphae. At relatively low concentrations, Mass A induced encystment of zoospores. It had no effect on the chemotactic response of zoospores but did adversely affect zoospore autoaggregation. A loss-of-function transformant of P. infestans lacking the G-protein α subunit was more sensitive to Mass A, whereas a gain-of-function transformant required a higher Mass A concentration to interfere with zoospore aggregation. Results indicate that Mass A disturbs various developmental stages in the life cycle of P. infestans and suggest that the cellular responses of P. infestans to this CLP are, in part, dependent on G-protein signaling.
Use of a Mixture of Surrogates for Infectious Bioagents in a Standard Approach to Assessing Disinfection of Environmental Surfaces

ABSTRACT We used a mixture of surrogates (Acinetobacter baumannii, Mycobacterium terrae, hepatitis A virus, and spores of Geobacillus stearothermophilus) for bioagents in a standardized approach to test environmental surface disinfectants. Each carrier containing 10 μl of mixture received 50 μl of a test chemical or saline at 22 ± 2°C. Disinfectant efficacy criteria were ≥6 log10 reduction for the bacteria and the spores and ≥3 log10 reduction for the virus. Peracetic acid (1,000 ppm) was effective in 5 min against the two bacteria and the spores but not against the virus. Chlorine dioxide (CD; 500 and 1,000 ppm) and domestic bleach (DB; 2,500, 3,500, and 5,000 ppm) were effective in 5 min, except for sporicidal activity, which needed 20 min of contact with either 1,000 ppm of CD or the two higher concentrations of DB.
The Tale of a Neglected Energy Source: Elevated Hydrogen Exposure Affects both Microbial Diversity and Function in Soil

ABSTRACT The enrichment of H2-oxidizing bacteria (HOB) by H2 generated by nitrogen-fixing nodules has been shown to have a fertilization effect on several different crops. The benefit of HOB is attributed to their production of plant growth-promoting factors, yet their interactions with other members of soil microbial communities have received little attention. Here we report that the energy potential of H2, when supplied to soil, alters ecological niche partitioning of bacteria and fungi, with multifaceted consequences for both generalist and specialist microbial functions. We used dynamic microcosms to expose soil to the typical atmospheric H2 mixing ratio (0.5 ppmv) permeating soils, as well as mixing ratios comparable to those found at the soil-nodule interface (10,000 ppmv). Elevated H2 exposure exerted direct effects on two HOB subpopulations distinguished by their affinity for H2 while enhancing community level carbon substrate utilization potential and lowering CH4 uptake activity in soil. We found that H2 triggered changes in the abundance of microorganisms that were reproducible yet inconsistent across soils at the taxonomic level and even among HOB. Overall, H2 exposure altered microbial process rates at an intensity that depends upon soil abiotic and biotic features. We argue that further examination of direct and indirect effects of H2 on soil microbial communities will lead to a better understanding of the H2 fertilization effect and soil biogeochemical processes. IMPORTANCE An innovative dynamic microcosm chamber system was used to demonstrate that H2 diffusing in soil triggers changes in the distribution of HOB and non-HOB. Although the response was uneven at the taxonomic level, an unexpected coordinated response of microbial functions was observed, including abatement of CH4 oxidation activity and stimulation of carbon turnover. Our work suggests that elevated H2 rewires soil biogeochemical structure through a combination of direct effects on the growth and persistence of HOB and indirect effects on a variety of microbial processes involving HOB and non-HOB.
Resignifying the sickle cell gene: Narratives of genetic risk, impairment and repair

Connecting theoretical discussion with empirical qualitative work, this article examines how sickle cell became a site of public health intervention in terms of ‘racialised’ risks. Historically, sickle cell became socio-politically allied to ideas of repair, in terms of the state improving the health of a neglected ethnic minority population. Yet, we elucidate how partial improvements in care and education arose alongside preventative public health screening efforts. Using qualitative research based in the United Kingdom, we show how a focus on collective efforts of repair can lie in tension with how services and individuals understand and negotiate antenatal screening. We illustrate how screening for sickle cell disorder calls into question narrative identity, undoing paradigms in which ethnicity, disablement and genetic impairment become framed. Research participants noted that rather than ‘choices’, it is ‘risks’ and their negotiation that are a part of discourses of modernity and the new genetics. Furthermore, while biomedical paradigms are rationally and ethically (de)constructed by participants, this was never fully engaged with by professionals, contributing to overall perception of antenatal screening as disempowering and leading to disengagement.
Production of the Antimicrobial Secondary Metabolite Indigoidine Contributes to Competitive Surface Colonization by the Marine Roseobacter Phaeobacter sp. Strain Y4I

ABSTRACT Members of the Roseobacter lineage of marine bacteria are prolific surface colonizers in marine coastal environments, and antimicrobial secondary metabolite production has been hypothesized to provide a competitive advantage to colonizing roseobacters. Here, we report that the roseobacter Phaeobacter sp. strain Y4I produces the blue pigment indigoidine via a nonribosomal peptide synthase (NRPS)-based biosynthetic pathway encoded by a novel series of genetically linked genes: igiBCDFE. A Tn5-based random mutagenesis library of Y4I showed a perfect correlation between indigoidine production by the Phaeobacter strain and inhibition of Vibrio fischeri on agar plates, revealing a previously unrecognized bioactivity of this molecule. In addition, igiD null mutants (igiD encoding the indigoidine NRPS) were more resistant to hydrogen peroxide, less motile, and faster to colonize an artificial surface than the wild-type strain. Collectively, these data provide evidence for pleiotropic effects of indigoidine production in this strain. Gene expression assays support phenotypic observations and demonstrate that igiD gene expression is upregulated during growth on surfaces. Furthermore, competitive cocultures of V. fischeri and Y4I show that the production of indigoidine by Y4I significantly inhibits colonization of V. fischeri on surfaces. This study is the first to characterize a secondary metabolite produced by an NRPS in roseobacters.
UV control of electric/magnetic properties of transition metal oxides grown on SrTiO3 single crystal

SrTiO3 (STO) shows notable photoconductivity by irradiation of UV light of 387 nm or shorter wavelength. The photoconductivity is caused by the excitation of valence electron to the conduction band beyond the band gap of 3.2 eV. It has been reported that the STO photocarrier can control the electric and magnetic properties of (La, Sr)MnO3 (LSMO) via transferring the STO photocarrier to the LSMO beyond the hetero-interface. We propose that the electronic properties of various perovskite type oxides can be controlled by the photocarrier transferred from STO. We have found the resistance increase of LaFeO3 the under UV irradiation.
Interaction of Mycobacterium ulcerans with Mosquito Species: Implications for Transmission and Trophic Relationships

ABSTRACT Mycobacterium ulcerans is the causative agent of Buruli ulcer, a severe necrotizing skin disease that causes significant morbidity in Africa and Australia. Person-to-person transmission of Buruli ulcer is rare. Throughout Africa and Australia infection is associated with residence near slow-moving or stagnant water bodies. Although M. ulcerans DNA has been detected in over 30 taxa of invertebrates, fish, water filtrate, and plant materials and one environmental isolate cultured from a water strider (Gerridae), the invertebrate taxa identified are not adapted to feed on humans, and the mode of transmission for Buruli ulcer remains an enigma. Recent epidemiological reports from Australia describing the presence of M. ulcerans DNA in adult mosquitoes have led to the hypothesis that mosquitoes play an important role in the transmission of M. ulcerans. In this study we have investigated the potential of mosquitoes to serve as biological or mechanical vectors or as environmental reservoirs for M. ulcerans. Here we show that Aedes aegypti, A. albopictus, Ochlerotatus triseriatus, and Culex restuans larvae readily ingest wild-type M. ulcerans, isogenic toxin-negative mutants, and Mycobacterium marinum isolates and remain infected throughout larval development. However, the infections are not carried over into the pupae or adult mosquitoes, suggesting an unlikely role for mosquitoes as biological vectors. By following M. ulcerans through a food chain consisting of primary (mosquito larvae), secondary (predatory mosquito larva from Toxorhynchites rutilus septentrionalis), and tertiary (Belostoma species) consumers, we have shown that M. ulcerans can be productively maintained in an aquatic food web.
Antibacterial effects of carvacrol against Staphylococcus aureus and Escherichia coli O157:H7

The objective of this study was to investigate the antimicrobial effects of carvacrol (CV) against Staphylococcus aureus (S. aureus) and Escherichia coli O157:H7 (E. coli O157:H7) strains in milk. The minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of CV against S. aureus and E. coli O157:H7 were determined. In addition, bactericidal kinetics and antimicrobial activity of CV against the aforementioned pathogens in milk over a period of 2 weeks were investigated. CV exhibited antibacterial activity against both foodborne pathogens tested. The MIC and MBC of CV against S. aureus were 15.0 and 20 ㎎/mL, respectively, whereas those against E. coli O157:H7 were 16.0 and 32 ㎎/mL, respectively. In time-kill assays, CV at MBC reduced the number of S. aureus and E. coli O157:H7 in milk to undetectable levels within 24 hr. The antibacterial effects of CV persisted for 14 days without any loss of activity. Results of this study suggest that CV has a potential antibacterial activity against foodborne pathogens such as S. aureus and E. coli O157:H7 in milk.
Determination of Dielectric Constant of Fabric Materials and Their Use as Substrates for Design and Development of Antennas for Wearable Applications

A novel approach to measure the dielectric constant of fabric substrate materials used for the development of wearable antennas (also called textile antennas) is presented in this paper. The technique reported here is based on the resonance method and focused on the use of microstrip patch radiator, which contains fabric material as its substrate. The accurate value of the dielectric constant of the fabric material can easily be extracted from the measured resonant frequency of the patch radiator. The dielectric constant values of six fabric materials, including jeans cotton, polyester combined cotton, and polyester, have been determined by this way. As an extended objective of this paper, initial investigations are done to study the performance/behavioral characteristics of wearable antennas in the Bluetooth industrial, scientific, and medical band. Two of the six textile antenna structures, developed to meet out the primary objective of determining the dielectric constant of fabrics, are tested, and their performance characteristics, such as impedance bandwidth, gain, efficiency, etc., are measured. In addition, another Bluetooth antenna employing polyester fabric substrate is designed considering its measured accurate value of dielectric constant and subjected to radiation pattern measurements. In general, all the measured antennas yield very good results, fulfilling the requirements for practical applications, and in particular, the third fabric antenna utilizing the accurate value of the dielectric constant determined shows superior performance characteristics compared to others, indicating the correctness of our approach. Thus, the suitability of fabric substrate materials for the development of textile antennas with microstrip patch configuration is also well demonstrated.
Efeito da corrente interferencial, 2000Hz, no limiar de dor induzida

O objetivo do estudo foi verificar a eficacia da corrente interferencial na diminuicao da dor frente a um estimulo doloroso pressorico e ao frio, em individuos saudaveis, utilizando tecnica de aplicacao bipolar sobre a raiz nervosa. Quatorze voluntarios participaram deste estudo e foram divididos em dois grupos. No primeiro dia, um grupo foi submetido a corrente interferencial enquanto o outro nao realizou a terapia. No dia seguinte, os grupos foram trocados. Foram avaliados os limiares da dor por pressao, com um algometro, e o da dor ao frio, alem do desconforto pela Escala Visual de Escala da Dor (EVAD). Como forma de eletroestimulacao, foi utilizada a corrente interferencial com os parâmetros 2.000Hz de corrente base, com AMF de 100Hz, ΔF 50%, slope 1/1. Os individuos foram reavaliados em tres momentos: logo apos eletroestimulacao, 20 minutos apos e uma hora depois. Quanto a comparacao entre as avaliacoes com o algometro de pressao e desconforto ao frio, os resultados nao foram significativos, tanto no grupo placebo quanto no grupo eletroestimulado. Nas avaliacoes do limiar da dor ao frio houve diminuicao do limiar de dor no grupo eletroestimulado. Conclui-se que o uso da corrente interferencial, com os parâmetros utilizados, nao produziu alteracao no limiar de dor a pressao nem no desconforto ao frio, mas produziu diminuicao do limiar de dor ao frio.
A metadata model for capturing presentations

We describe the design of a metadata model for capturing presentations developed as part of the VACE project (video and audio capturing and embedding). VACE is a modular, open, distributed framework for capturing presentations like lectures by using standard presentation and publishing tools for different media types. Different media formats can be used in one recording session in order to suit the needs of different presentation types, e.g. slides plus the talk of a lecturer. Metadata are necessary to combine these media data in an efficient way. The combination of content based and synchronisation metadata is utilized for the integration of recorded material e.g. in Web based learning systems, to provide navigation and search functions but can also be used for other post production purposes, e.g. video editing or DVD authoring.
Competitive Oxidation of Volatile Fatty Acids by Sulfate- and Nitrate-Reducing Bacteria from an Oil Field in Argentina

ABSTRACT Acetate, propionate, and butyrate, collectively referred to as volatile fatty acids (VFA), are considered among the most important electron donors for sulfate-reducing bacteria (SRB) and heterotrophic nitrate-reducing bacteria (hNRB) in oil fields. Samples obtained from a field in the Neuquén Basin, western Argentina, had significant activity of mesophilic SRB, hNRB, and nitrate-reducing, sulfide-oxidizing bacteria (NR-SOB). In microcosms, containing VFA (3 mM each) and excess sulfate, SRB first used propionate and butyrate for the production of acetate, which reached concentrations of up to 12 mM prior to being used as an electron donor for sulfate reduction. In contrast, hNRB used all three organic acids with similar kinetics, while reducing nitrate to nitrite and nitrogen. Transient inhibition of VFA-utilizing SRB was observed with 0.5 mM nitrite and permanent inhibition with concentrations of 1 mM or more. The addition of nitrate to medium flowing into an upflow, packed-bed bioreactor with an established VFA-oxidizing SRB consortium led to a spike of nitrite up to 3 mM. The nitrite-mediated inhibition of SRB led, in turn, to the transient accumulation of up to 13 mM of acetate. The complete utilization of nitrate and the incomplete utilization of VFA, especially propionate, and sulfate indicated that SRB remained partially inhibited. Hence, in addition to lower sulfide concentrations, an increase in the concentration of acetate in the presence of sulfate in waters produced from an oil field subjected to nitrate injection may indicate whether the treatment is successful. The microbial community composition in the bioreactor, as determined by culturing and culture-independent techniques, indicated shifts with an increasing fraction of nitrate. With VFA and sulfate, the SRB genera Desulfobotulus, Desulfotignum, and Desulfobacter as well as the sulfur-reducing Desulfuromonas and the NR-SOB Arcobacter were detected. With VFA and nitrate, Pseudomonas spp. were present. hNRB/NR-SOB from the genus Sulfurospirillum were found under all conditions.