Miracle-dz/UniFAR-FTUs huggingface dataset:
The hydrophobic contacts between the center of the beta I domain and the alpha 1/alpha 7 helices are crucial for the low-affinity state of integrin alpha(4)beta(7)

Integrin alpha(4)beta(7) mediates both rolling and firm adhesion of lymphocytes by modulating its affinity to the ligand: mucosal addressin cell adhesion molecule-1 (MAdCAM-1). Integrin activation is associated with allosteric reshaping in the beta subunit I (beta I) domain. A prominently conformational change comprises displacement of the alpha 1 and alpha 7 helices in the beta I domain, suggesting that the location of these helices is important for the change in integrin affinity. In the present study, we report that the hydrophobic contacts between the center of the beta I-7 domain and the alpha 1/alpha 7 helices play critical roles in keeping alpha(4)beta(7) in a low-affinity state. Using molecular dynamics simulation, we identified nine hydrophobic residues that might be involved in the critical hydrophobic contacts maintaining integrin in a low-affinity state. Integrin beta 7I domain exhibited a lower binding free energy for ligand after disrupting these hydrophobic contacts by substituting the hydrophobic residues with Ala. Moreover, these alpha(4)beta(7) mutants not only showed high-affinity binding to soluble MAdCAM-1, but also demonstrated firm cell adhesion to immobilized MAdCAM-1 in shear flow and enhanced the strength of the alpha(4)beta(7)-MAdCAM-1 interaction. Disruption of the hydrophobic contacts also induced the active conformation of alpha(4)beta(7). Thus, the findings obtained in the present study reveal an important structural basis for the low-affinity state of integrin.
The Beta Subunit of the AP-1 Complex is Essential for Hair Cell Function and Basolateral Targeting of NKA

TMHS is a known human deafness gene found in the DNFB67 locus that may play a role in hair cell mechanotransduction. In this study, we show that the highly conserved zebrafish ortholog, tmhsa, plays a similar role in zebrafish hair cells. Through analysis of the astronaut mutant, we observe that the tmhsa gene is essential for hair-cell physiology, specifically hair cells of the inner ear as we saw no defects in lateral-line hair-cells. This suggests that a different gene may play a similar role in hair cells of the lateral-line neuromasts. We also demonstrate that expression of tmhsa solely in hair cells is sufficient to rescue the zebrafish tmhsa mutant.
Avaliação da qualidade nos serviços hospitalares: Estudo de caso

iv Lista de Figuras . viii Lista de Gráficos . ix Lista de Quadros . x Lista de Tabelas . xi Capítulo I – Introdução . 1 1.1 Motivação . 1 1.2 Formulação do problema . 2 1.3 Questão de Investigação . 2 1.4 Hipóteses . 3 1.5 Metodologia . 6 1.6 Abrangência da Investigação . 7 1.7 Estrutura . 7 Capítulo II – Enquadramento Teórico . 9 2.1 Introdução . 9 2.2 O conceito de qualidade nos serviços . 9 2.3 Qualidade nos serviços vs. Qualidade nos bens . 13 2.4 Medição da qualidade nos serviços . 15 2.5 Avaliação das dimensões da qualidade nos serviços . 17 2.5.1 Origem das dimensões da qualidade . 17 2.5.2 Metodologia do SERVQUAL . 20 Avaliação da qualidade nos serviços hospitalares: Estudo de caso vi 2.5.3 Críticas ao SERVQUAL . 21 2.6 SERVPERF como alternativa ao SERVQUAL . 24 2.7 Conclusões . 25 Capítulo III – Metodologia . 26 3.1 Introdução . 26 3.2 Objectivos da investigação . 26 3.3 Desenvolvimento da investigação . 27 3.3.1 Estudo de caso . 27 3.3.2 Locais de estudo . 28 3.3.3 Técnicas de recolha de dados . 28 3.4 Processo de Amostragem . 29 3.5 Tratamento e análise dos dados . 30 3.6 Validade . 32 Capítulo IV – Análise e Discussão dos Resultados . 33 4.1 Introdução . 33 4.2 Caracterização do caso de estudo . 33 4.3 Caracterização da amostra . 34 4.3.1 Idade . 34 4.3.2 Grau de Escolaridade . 35 4.3.3 Distância ao serviço de saúde. 36 4.4 Avaliação global das dimensões da qualidade . 37 4.5 Avaliação individual de cada dimensão da qualidade . 37 4.5.1 Tangibilidade . 38 4.5.2 Confiança . 40 4.5.3 Capacidade de Resposta . 43 4.5.4 Garantia . 46 Avaliação da qualidade nos serviços hospitalares: Estudo de caso vii 4.5. 5 Empatia. 48 4.6 Avaliação das dimensões da qualidade por variável independente . 51 4.7 Conclusões . 63 Capítulo V – Conclusões e Recomendações . 64 5.1 Resumo das hipóteses . 64 5.2 Discussão dos resultados . 68 5.3 Limites da Investigação . 73 5.4 Perspectivas para trabalho futuro . 74 ANEXO 1 . 75 ANEXO 2 . 80 ANEXO 3 . 84 Bibliografia . 86 Avaliação da qualidade nos serviços hospitalares: Estudo de caso viii Lista de Figuras Figura 2.1. Modelo Conceptual para a Qualidade do Serviço de Parasuraman et al., 1985 (adaptado). Avaliação da qualidade nos serviços hospitalares: Estudo de caso ix Lista de Gráficos Gráfico 4.1. Percentagem de observações por intervalo de idade. Gráfico 4.2. Percentagem de observações por intervalos relativos ao grau de escolaridade. Gráfico 4.3. Percentagem de observações por intervalo de distância, em quilómetros. Gráfico 4.4. Plot da variável dependente P1. , quanto à variável independente Distância. Gráfico 4.5. Plot da variável dependente P20. , quanto à variável independente Distância. Avaliação da qualidade nos serviços hospitalares: Estudo de caso
Major pathway of rRNA processing in the nucleolus

Unknown nucleases process the 32S precursor rRNA (pre-rRNA) at site 4, yielding the 28S rRNA and the 12S pre-RNA that will be further processed to the mature 5.8S rRNA. Processing occurs in precursor 60S ribosomal (pre-60S) subunits that contain ribosomal proteins and processing factors. The PES1:BOP1:WDR12 complex (PeBoW complex) associates with pre-60S subunits in both the nucleus and cytosol where it is involved in processing 32S rRNA and recycling pre-60S subunit processing factors. required PELP1:TEX10:WDR18
Across-Breed Imputation with Whole Genome Sequence Data in Dairy Cattle

Here we use BTA25 to assess imputation accuracy using different reference scenarios. Sequence information of 50 Brown Swiss animals was masked to mimic that of medium and high density SNP chips. The full sequence was then imputed, and the percentage of correctly imputed loci was assessed. The correlation between true and imputed genotypes was also calculated. 1 Bern University of Applied Sciences, Switzerland 2 Qualitas AG, Switzerland 3 Iowa State University, United States of America, 4 agn Genetics, Switzerland
Textstrategische Funktion indefiniter Komplement-NPs am Satzanfang. Eine kontrastive Untersuchung Dt./Frz.

: Sprachübergreifend kommen satzeröffnenden Satzgliedern textver-knüpfende Funktionen zu – was oft durch Wiederaufnahme schon eingeführter bzw. zugänglicher Informationskomponenten geleistet wird. Am Satzanfang ist also eher Definitheit erwartbar. Trotzdem finden sich sowohl im Deutschen als auch im Französischen zu viele indefinite NPs, als dass man sie als nicht signifikante Ausnahmen betrachten könnte. Im vorliegenden Beitrag wird eine kontrastive Korpus-Untersuchung solcher satzeröffnenden indefiniten NPs vorgenommen, wobei der Fokus auf indefiniten Komplement-NPs liegt. Zunächst wird eine Typologie der häufigsten NP-Typen in den beiden Kontrastsprachen aufgestellt, bevor in einem zweiten Teil präferierte Kombinationen beschrieben werden. Im dritten Teil wird auf die textstrategischen Funktionen dieser NPs eingegangen und gezeigt, dass sie trotz Unterschiede hinsichtlich der grammatischen Funktion zur Erfüllung derselben Textfunktionen eingesetzt werden: Textverknüpfung, Serialisierung, Einführung eines neuen Teilthemas, Rahmensetzung. Abstract (fr.): En français comme en allemand, les unités en tête d’énoncé jouent un rôle dans la structuration du texte, ce qui passe souvent par la reprise d’informations déjà accessibles en contexte. On peut donc s’attendre à trouver, ; / analyse; Archäologie / archéologie > Ausgrabungen / fouilles usw.
Salinity Effects on Germination, Growth, and Yield of Turnips

The effect of salinity on germination, growth, and yield of turnips (Brassica rapa L. Rapifera group) was determined in artificially salinized laboratory germination cultures and field plots. Saline treatments were imposed by irrigating with water that contained equal weights of NaCl and CaCl2. Relative yield of turnip tops (greens) was reduced 4.3% with each unit increase in salinity above 3.3 dS/m (dS/m = mmho/cm). Storage root growth was reduced 9.0% for each unit increase in salinity above 0.9 dS/m. Top growth would be considered moderately tolerant to salinity and root growth as moderately sensitive. Turnips proved to be more salt-tolerant at germination than at later stages of growth. Root quality was not affected over the salinity range tested.
Application of metabolomics to identify metabolite patterns showing the importance of dietary carbohydrate source in neonatal rats

ii PREFACE . iv TABLE OF CONTENTS . v LIST OF TABLES . viii LIST OF FIGURES . ix LIST OF ABBREVIATIONS. xiii ACKNOWLEDGEMENTS . xvi DEDICATION . xvii CHAPTER 1: INTRODUCTION . 1 Lactose composition, digestion and absorption . 5 Differences between galactose and glucose metabolism . 7 Galactose metabolism . 8 Glucose metabolism . 12 Metabolomics workflow . 18 Analytical techniques . 19 Ion separation, ion sorting and detection . 20 vi Target compound analysis and targeted metabolomics . 22 Principal component analysis . 24 CHAPTER 2: STUDY DESIGN AND METHODS . 31 Rat milk formula composition and preparation . 33 Gastrostomy experiment . 35 Rearing of rat pups . 35 Tissue and biofluid collection . 37 Target compound analysis . 39 Preparation of liver, plasma and urine samples . 41 Instruments and analysis . 44 Targeted metabolomics . 47 Principal component analysis . 47 CHAPTER 3: RESULTS . 51
Toward a Molecular Classification of Peripheral T-Cell Lymphomas: The Role of Gene Expression Profiling

Peripheral T-cell lymphomas-not otherwise specified (PTCL/NOS) are the most common T-cell neoplasms. This study sought to reshape the PTCL/NOS sub-classification (including its two main morphological variants, Lennert lymphoma, LL, and Follicular variant, F-PTCL) based on the correspondence between their molecular features and those of different functional T-cell subsets, also assessing the clinical impact of such an approach. We found that PTCLs/NOS could be divided into groups corresponding to T-cell subsets differently reliant on transcription regulators including mTOR and FOXP3, and identified minimal gene sets discriminating among these groups. Notably, by grouping tumors according to their dependency on master regulators of T-lymphocyte fate, we identified three groups (T-cytotoxic, Treg/TFH, and other-T-helper) characterized by specific genetic patterns and significantly different clinical outcomes. Immunohistochemistry partially substituted for the molecular analysis by consistently recognizing only Treg and TFH cases. Finally, targeted inhibition of MTOR in T-helper cases (that were characterized by genetic lesions targeting the pathway) was proved to be effective ex vivo. We conclude that PTCL/NOS can be divided into subgroups corresponding to different cellular counterparts, characterized by different genetic patterns and possibly sensitivity to specific therapeutic approaches. Furthermore, we identified different gene and microRNA signatures for LL capable of differentiating it from other PTCL/NOS and enriched in cytotoxic function. Moreover, PI3K/Akt/mTOR pathway emerged as novel therapeutic targets for LL. Additionally, LL showed some differences with other PTCL/NOS in terms of clinical features, all supporting its recognition as a distinct entity. Besides, we found that F-PTCL has a distinct molecular signature more similar to PTCL/NOS rather than AITL, and therefore cannot be included among AITLs at least based on GEP, although this necessities more genetic studies. Overall, these results may impact on PTCL classification as well as on future studies aimed to define the more appropriate therapeutic strategy for each identified subgroup/entity.
Evaluation of the effect of a highly soluble calcium source in broiler diets supplemented with phytase on performance, nutrient digestibility, foot ash, mobility and leg weakness

High inclusion concentrations of dietary calcium (Ca) from limestone may have detrimental effects on broiler performance, such as increased gastric pH and a reduction in phosphorus (P) and amino acid digestibility. To assess the potential for feeding diets with lower total Ca concentrations, the effect of a novel highly soluble source of calcium (HSC) on bird performance, nutrient digestibility and skeletal integrity of 1120 male Cobb-500 broilers was compared with that of limestone. Eight dietary treatments in a 2 × 2 × 2 factorial design consisted of two sources of Ca (limestone or HSC), two concentrations of Ca (6.0 or 7.7 g/kg in the starter and 4.0 or 5.7 g/kg in the grower), two phytase levels (0 or 500 FTU/kg). The effect of dietary treatments on growth performance and feed intake were measured from Day 1 to Day 40 and foot ash and leg health on Day 40. At the end of the starter period, in the absence of phytase, changing Ca source from limestone to HSC at 6.0 g/kg total Ca decreased both bodyweight gain and feed intake (P < 0.05). The addition of phytase to diets containing 6.0 g/kg total Ca provided by HSC increased bodyweight gain (P < 0.05), as well as increasing feed intake (P < 0.05). At the end of the grower period, diets supplemented with phytase increased bodyweight gain (P < 0.001), birds fed the higher Ca concentration were also observed to have an increased bodyweight gain (P < 0.001). Apparent ileal Ca digestibility significantly increased with the addition of phytase to diets containing 4.0 g/kg total Ca provided by HSC (P < 0.05), this was also observed for P digestibility. Replacement of limestone with HSC when fed in conjunction with phytase has the potential to be an effective strategy to improve broiler performance and bone mineralisation.
Essential Oil Composition of Four Magnoliaceae Species Cultivated in Vietnam

ABSTRACT The chemical constituents of hydrodistilled essential oils from the leaves of four Magnoliaceae species were examined. The main compounds in Manglietia fordiana oils were δ-cadinene (18.0%), (E)-nerolidol (16.7%), α-copaene (12.8%), α-selinene (9.1%), and limonene (8.3%); in Manglietia conifera oils they were β-caryophyllene (29.9%), α-humulene (7.4%), (E)-nerolidol (6.3%), and caryophyllene oxide (5.5%). The major constituents of Michelia mediocris oil were (E)-nerolidol (36.4%), δ-cadinene (10.9%), and β-selinene (7.2%), while in Michelia tonkinensis they were α-pinene (40.3%), β-phellandrene (7.6%), β-pinene (7.4%), and bicyclogermacrene (7.3%).
Leukosit Ayam Pedaging setelah Diberikan Paracetamol

Penelitian ini bertujuan untuk mengetahui profil hematologi (total leukosit dan diferensial leukosit) pada ayam pedaging yang diberikan paracetamol dalam pakan mulai umur 14 – 35 hari, Penelitian ini menggunakan Rancangan acak lengkap yaitu P0 (kelompok ayam pedaging yang hanya diberi pakan standar SB-11), P? (pakan standar SB-11 dan paracetamol 1 g/kg pakan), P? (pakan standar SB-11 dan paracetamol 2 g/kg pakan), P? (pakan standar SB-11 dan paracetamol 4 g/kg pakan) masing-masing kelompok terdiri dari enam ekor ayam pedaging. Pengambilan darah pada vena brachialis dilakukan sebelum diberikan perlakuan dan pada hari ke 21 setelah diberikan perlakuan. Hasil penelitian menunjukkan bahwa pemberian paracetamol dosis 1-4 g/kg pakan tidak berpengaruh nyata terhadap total leukosit (P>0,05) akan tetapi hanya berpengaruh nyata terhadap monosit (P<0,05) namun masih dalam rentang normal. Disimpulkan bahwa paracetamol tidak berpengaruh nyata terhadap total leukosit dan diferensial leukosit ayam pedaging.
Ononis natrix and Salvia verbenaca: Two Jordanian Medicinal Plants with Cytotoxic and Antibacterial Activities

ABSTRACT The phytochemical constituents and antibacterial and cytotoxic activities of Ononis natrix and Salvia verbenaca were appraised. Ethyl acetate extracts of these plants tested positive for flavonoids and terpenes and negative for alkaloids. The extracts inhibited growth of the gram-positive Bacillus subtilis and B. brevis (MIC 12.5–50 µg.mL–1) and were cytotoxic to the MDA MB-231 breast cancer cell line (IC50 29–41 µg.mL–1).
Further European integration: mixed sub-species colonies of great cormorants Phalacrocorax carbo in Britain - colony establishment, diet, and implications for fisheries management

Inland colonies of Great Cormorants Phalacrocorax carbo in Britain contain individuals of both European subspecies the Atlantic Great Cormorant P.c. carbo and the Continental Great Cormorant P.c. sinensis. Not only are the two breeding together, they are probably hybridising. Colony size and diet were studied in the three oldest and largest of these colonies: Abberton Reservoir, Little Paxton and Besthorpe. Abberton was founded in 1981 and increased steadily to 526 nests in 1993, thereafter numbers varied and averaged 464 nests per annum with a peak count of 551 in 1996. This colony, the largest inland one in Britain, is 7 km from the sea and breeding birds foraged mostly in estuarine habitat. Diet was diverse (at least 26 marine and freshwater fishes) but dominated by Eel Anguilla anguilla and flatfishes Pleuronectidae. Calculations suggest that breeding birds here consumed 70.3 tonnes of fish in 1993. Colony-size trajectories for the other two colonies were similar to Abberton but with maximum counts of around 200 pairs. Diet at these colonies, both > 60km from the coast, comprised exclusively freshwater fish, mostly cyprinids and particularly Roach Rutilus rutilus. Many of the English inland colonies seem to have formed as separate 'colonisation' events rather than following Van Eerden & Gregersen's (1995) mother-satellite colony formation model for the expansion of Continental Great Cormorants in mainland Europe. Presumably such events in Britain were, at least in part, facilitated because birds were already roosting in many different places, and by the large number of non-breeding birds of continental origin. We speculate on the implications of inland mixed sub-species Great Cormorant colonies in Britain for the future management of both Great Cormorants and fisheries.
Estudio de una vacuna atenuada de Anaplasma marginale en México

Este informe describe el segundo de una serie de estudios efectuados en Mexico con respecto a la inocuidad y potencia de la vacuna atenuada de Anaplasma marginale de la universidad de Illinois. En el esperimento se incluyen 20 bovinos Hereford de 3 anos de edad. Diez de los animales fueron vacunados y los restantes se dejaron sin vacunar como testigos. Todos los animales se mantuvieron en aunidades de aislamiento en el Instituto Nacional de Investigaciones Pecuarias (I.N.I.P) en la ciudad de Mexico. La alimentacion proporcionada a los animales es la que se recomienda para alimentar ganado adulto de carne en el periodo de engorda. A los 5 Meses y una semana despues de la vacunacion de los animales fueron desafiados con una cepa virulenta de Anaplasma marginale aislada en Mexico. La cepa del desafio se mantuvo, en alicuotas de 1.0 ml en nitrogeno liquido. Se conoc la dosis suficiente para causar 100% de morbilidad y 80% de mortalidad y el 70% de proteccion para mortalidad en el grupo de animales vacunos, comparados con 100% de mortalidad de un grupo de control. La morbilidad en el 30% de los animales fue de corta duracion y de rapida recuperacion.
Effects of Nonylphenol on Hyperadrenalism and Metabolism in Male Rats

Nonylphenol (NP) is an environmental endocrine-disrupting chemical (EDC) that has been detected in human cord blood and milk. It is unavoidable that human fetus and infant exposure to this environmental contaminant. We previously observed that developmental NP exposure led to increased body weight, elevated plasma adrenocorticotropin (ACTH), higher production and concentrations of corticosterone and aldosterone, and more 11b-hydroxysteroid dehydrogenase I (11b-HSD1) expression/activity during the first generation at the adult stage. The “fetal origins of adult disease” phenomenon appears to be based on epigenetic modifications during the sensitive developmental period. Epigenetic modifications during development can be “inherited” after differentiation by DNA methyltransferases. Without intervention, NP-induced Cushing’s syndrome can impact the health of an individual for life. We have reported that recovering from NP-induced Cushing’s syndrome requires one full generation for male rats (F2 generation recovers from the NP-affected F1 generation). The logical treatment plan for the F1 generation consists of counteracting the elevated 11β-HSD1 activity because epigenetic modifications cannot be effectively reversed. The inhibition of 11b-HSD1 activity has become a potential treatment plan. PF915275 (PF; 4-cyano-biphenyl-4-sulfonic acid (6-amino-pyridin-2-yl)-amide) is a selective inhibitor of 11b-HSD1, and an initial clinical trial has proven its safety and efficacy. We have reported that PF915275 can reverse/alleviate NP-induced Cushing’s syndrome. This study showed a potential direction for the treatment of ENDR-induced Cushing’s syndrome and pandemic metabolic syndrome.
Competition Between Triazine-Resistant and -Susceptible Common Waterhemp (Amaranthus rudis)

The relative competitiveness of triazine-resistant (TR) and -susceptible (TS) common waterhemp, as a component of fitness, was assessed using TR and TS biotypes collected from the same field in Fillmore County, Nebraska. Competition between biotypes was evaluated under greenhouse conditions using a set of replacement series experiments with five ratios of TR:TS (1:0, 3:1, 1:1, 1:3, and 0:4) and four plant densities (50, 100, 150, and 300 plants m−2). No difference in final individual plant weight between TR and TS plants was observed in the absence of interbiotypic competition at all plant densities examined. Interbiotypic competition was indicated by ANOVA of plant weight and modified relative crowding coefficients (RCC) for plant weight, height, and volume. Replacement series diagrams showed TS plants were more competitive than TR plants, as measured by plant weight at densities of 50, 100, and 150 plants m−2. TS and TR plants were equally competitive at 300 plants m−2. Individual TS plant weight for 50, 100, and 150 plants m−2 was greatest at the 3:1 ratio (TR:TS) and decreased as the proportion of susceptible plants increased. Individual TR plant weight for 50, 100, and 150 plants m−2 was greatest at the 1:0 ratio and decreased as the TR proportion decreased. Lack of interbiotypic competition at 300 plants m−2 and decreasing TS plant weight with increasing ratios suggest intrabiotypic competition. RCC values for volume and height were calculated weekly during the experiment and indicated the competitive advantage of TS plants was constant from transplanting through harvest.
Jusqu'où faut-il abaisser le LDL-cho

nir, en particulier en prévention secondaire, sont discutés depuis plusieurs années. Il n’existe aujourd’hui aucune étude scientifique permettant de répondre (selon l’evidence based medicine) à cette question. Toutefois, des éléments nouveaux de réflexion vont faire discuter les recommandations actuelles qui, en France, se fondent sur des seuils d’intervention sans donner des objectifs thérapeutiques.