Miracle-dz/UniFAR-FTUs huggingface dataset:
Design and Development of IoT Based Smart Aquaculture System in a Cloud Environment

Aquaculture is one of the crucial elements to help the nourishment needs of the world network, particularly in creating nations. Internet of Things (IoT) is a hot research subject, as showed by the expanding consideration and the extensive overall speculations dedicated to it. The observing of cultivating procedures can upgrade the utilization of assets and improve its supportability and productivity. It very well may be executed under various conditions. A few offices are sent in the ocean, while different offices are set inland. In inland offices, fish are kept in tanks that can change in size and materials. In offices that perform serious aquaculture, numerous endeavors are taken to augment the execution of the fish. In this paper, we propose a framework for developing an exceptionally beneficial aquaculture framework, equipped for preparing the employments of observing, wise control of the Internet of thing (IoT), irregularity mindfulness, and carbon outflow decrease just as vitality protection. And also includes water quality check, environmental monitoring, power monitoring, and web surveillance platform
EMERGENCE OF COAGULASE POSITIVE METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM BUFFALO MASTITIS MILK SAMPLES

The present study was undertaken to screen methicillin resistant Staphylococcus aureus (MRSA) in mastitis buffalo milk samples from northern India. A total of 120 buffalo mastitis milk samples were collected from individual quarter over a one year period mainly from animals reared by marginal farmers. The isolates were presumptively confirmed by growing the organism in selective media and biochemical tests. A total of 52 samples were presumptively confirmed as Staphylococci on the basis of growth in Mannitol Salt Agar (MSA); out of which 12 samples were coagulase positive Staphylococcus aureus (S. aureus) by growth in Baird Parker Agar (BPA) and tube coagulase test. Antibacterial profiling and growth on Methicillin resistant Staphylococcus aureus (MeReSa) Agar indicated five isolates as MRSA. Further PCR of specific gene segments and their subsequent characterization based on 16S ribosomal RNA, of staphylo-coagulase and mecA confirmed the findings. The study accentuates the importance of determining the causal agents of mastitis, antibacterial profiling so as to enable the clinicians and researchers to intervene and adopt effective control measures.
Comparison of Infrared Thermography and Heat Flux Method for Dynamic Thermal Transmittance Determination

This paper proposes an alternative experimental procedure that uses infrared thermography (IRT) for measuring the surface temperature of building elements, through which it is possible to approximate the thermal transmittance or the U-value. The literature review showed that all authors used similar procedures that require semi-stationary heat transfer conditions, which, in most cases, could not be achieved. The dynamic and the average methods that are given in ISO 9869 were also used with the IRT and the heat flux method (HFM). The dynamic method (DYNM) shows a higher level of accuracy compared to the average method (AVGM). Since the algorithm of the DYNM is more complicated than that of the AVGM, Microsoft Excel VBA was used to implement the algorithm of the DYNM. Using the procedure given in this paper, the U-value could be approximated within 0–30% of the design U-value. The use of IRT, in combination with the DYNM, could be used in-situ since the DYNM does not require stable boundary conditions. Furthermore, the procedure given in this paper could be used for relatively fast and inexpensive U-value approximation without the use of expensive equipment (e.g., heat flux sensors).
FIBRE REINFORCED CONCRETE ENTIRELY MADE FROM RECYCLED CONSTRUCTION WASTE , SYNTHETIC FIBRES , CEMENT AND WATER AND ITS PRACTICAL UTILISATION

Utilisation of construction and demolition waste in production of structural concretes is still questionable. Use of fibres for enhancement of material properties and rejection of uncertainties is a hopeful solution for use of the recycled materials as aggregate in concrete. The paper will introduce possibilities of combination of fibres and waste aggregate and examples of its use in practical applications. Complex approach to utilisation of construction waste is an interesting offer for building industry contactors. Procedure of production of fibre reinforced concrete with recycled aggregate and proposed manners of manufacturing for some structural applications will be presented. Results of experiments will be shown and way of testing will be discussed for particular applications. The economical benefits and cost effectiveness will be evaluated for particular practical applications.
Mechanistic investigation of domain specific unfolding of human serum albumin and the effect of sucrose

This study is devoted to understand the unfolding mechanism of a multidomain protein, human serum albumin (HSA), in absence and presence of the sucrose by steady‐state and time‐resolved fluorescence spectroscopy with domain specific marker molecules and is further being substantiated by molecular dynamics (MD) simulation. In water, the domain III of HSA found to unfold first followed by domains I and II as the concentration of GnHCl is increased in the medium. The sequential unfolding behavior of different domains of HSA remains same in presence of sucrose; however, a higher GnHCl concentration is required for unfolding, suggesting stabilizing effect of sucrose on HSA. Domain I is found to be most stabilized by sucrose. The stabilization of domain II is somewhat similar to domain I, but the effect of sucrose on domain III is found to be very small. MD simulation also predicted a similar behavior of sucrose on HSA. The stabilizing effect of sucrose is explained in terms of the entrapment of water molecules in between HSA surface and sucrose layer as well as direct interaction between HSA and sucrose.
Measure of k phases uncertainty

In this paper we suggest a refinement for the concept of uncertainty and establish the general measure method of uncertainty. We distinguish uncertainty and uncertain attribute; use vector theory to describe the independent attribute of uncertainty and construct existing modality of uncertainty; propose the measure models of k phases uncertainty corresponding to target degree and uncertain degree respectively, and make some normal points for these measures so that their values are comparable in degree. To discuss how to apply above measure models, we use the temperature in real weather report as a given demonstration, and the results show that the method proposed in this paper is effective and has quantitative form processed by computers, therefore it has a favorable application perspective.
Persistent immune activation in HIV-1 infection is associated with progression to AIDS

Background: HIV-1 infection is characterized by chronic generalized CD8 and CD4 T cell hyperactivation, the biological effect of which is not understood. Objective: To study the relation between chronic immune activation and CD4 T cell depletion in HIV-1 infection. Design: Prospective cohort study among participants of the Amsterdam Cohort Studies on HIV-1 infection and AIDS who have a known seroconversion date (n = 102). Methods: CD4 and CD8 T cell activation marker expression was analysed by FACScan before and after seroconversion (1 and 5 years after seroconversion); T cell proliferation and T cell numbers were also measured. Cox proportional hazard analyses were used to study the predictive value of these parameters for progression to AIDS. Results: Preseroconversion low CD4 T cell numbers or elevated levels of CD4 T cell activation were associated with increased risk for development of AIDS after HIV-1 seroconversion. Progression to AIDS was associated with loss of both CD4 and CD8 naive T cells. The predictive value of CD8 T cell activation was confirmed and, in addition, in the course of infection low CD4 T cell counts and increasing proportions of dividing CD4 T cells, dividing CD8 T cells or elevated CD4 T cell activation marker expression became independent predictors of progression to AIDS. Conclusions: Increased T cell activation has predictive value for HIV-1 disease progression even before seroconversion. These data support the hypothesis that persistent hyperactivation of the immune system may lead to erosion of the naive T cell pool and CD4 T cell depletion.
Comparison Between the Effectiveness of Complex Decongestive Therapy and Stellate Ganglion Block in Patients with Breast Cancer-Related Lymphedema: A Randomized Controlled Study.

BACKGROUND Breast cancer-related lymphedema (BCRL) of the upper extremities often follows breast cancer treatment. Although complex decongestive therapy (CDT) is currently the standard treatment for BCRL, stellate ganglion block (SGB) has also been reported to be effective. OBJECTIVES This study aimed to determine the effectiveness of SGB in the treatment of BCRL, and to assess the impact of the treatment on the quality of life (QoL) compared to CDT. STUDY DESIGN A randomized controlled trial. SETTING A single academic hospital, outpatient setting. METHODS A total of 38 patients with BCRL were recruited. Patients were randomly divided into 2 groups. Patients enrolled in the CDT group underwent 10 sessions of CDT for 2 weeks, whereas patients in the SGB group received 3 consecutive SGBs every 2 weeks. Changes in circumference, volume, and bioimpedance in the upper extremity were measured at baseline and 2 weeks after treatment and compared between the 2 groups. EuroQol-5 dimensions (EQ-5D) and EuroQol visual analog scale (EQ VAS) for QoL and subjective improvement were monitored. RESULTS In both groups, side-to-side difference of circumference after the treatment was decreased significantly from baseline (P < 0.05), and side-to-side difference of volume was reduced significantly in the SGB group (P < 0.05). No statistically significant difference was noted in the treatment effect between the 2 groups. Results of the EQ-5D, EQ VAS, and questionnaires regarding subjective symptoms administered at baseline and 2 weeks after each intervention revealed no statistically significant difference in the treatment effects between CDT and SGB. LIMITATIONS Further long-term follow-up studies with a greater number of patients that include analysis according to the severity and duration of symptoms are needed. CONCLUSIONS The results of this study suggest that SGB is an effective treatment for BCRL and may be considered as an alternative to CDT. KEY WORDS Stellate ganglion block, complex decongestive therapy, breast cancer, lymphedema, breast cancer-related lymphedema, quality of life, bioimpedance, secondary lymphedema.
In vitro Antileishmanial Activity of Pleumeria pudica Leaf Extracts on Leishmania donovani Promastigotes

The objective of the present study was to evaluate in vitro antileishmanial activity of leaves form Plumeria pudica Jacq. (Apocynaceae). In the present study, the in vitro antileishmanial activity of petroleum ether, chloroform and methanol extracts from P. pudica leaf was evaluated against Leishmania donovani (strain AG 83) promastigotes by in vitro promastigote cell toxicity assay by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide). Here, all extracts markedly inhibited the growth of L. donovani promastigotes in vitro in a concentration dependent manner. The methanol extract was the most active followed by chloroform and petroleum ether extracts. Therefore, from the present study it can be inferred that P. pudica leaf exhibited remarkable antileishmanial activity against Leishmania donovani promastigotes in vitro.
Hybrid Coronary Percutaneous Treatment with Metallic Stents and Everolimus-Eluting Bioresorbable Vascular Scaffolds: 2-Years Results from the GABI-R Registry

The limitations of the first-generation everolimus-eluting coronary bioresorbable vascular scaffolds (BVS) have been demonstrated in several randomized controlled trials. Little data are available regarding the outcomes of patients receiving hybrid stenting with both BVS and drug-eluting stents (DES). Of 3144 patients prospectively enrolled in the GABI-Registry, 435 (age 62 ± 10, 19% females, 970 lesions) received at least one BVS and one metal stent (hybrid group). These patients were compared with the remaining 2709 (3308 lesions) who received BVS-only. Patients who had received hybrid stenting had more frequently a history of cardiovascular disease and revascularization (p < 0.05), had less frequently single-vessel disease (p < 0.0001), and the lesions treated in these patients were longer (p < 0.0001) and more frequently complex. Accordingly, the incidence of periprocedural myocardial infarction (p < 0.05) and that of cardiovascular death, target vessel and lesion failure and any PCI at 24 months was lower in the BVS-only group (all p < 0.05). The 24-months rate of definite and probable scaffold thrombosis was 2.7% in the hybrid group and 2.8% in the BVS-only group, that of stent thrombosis in the hybrid group was 1.86%. In multivariable analysis, only implantation in bifurcation lesions emerged as a predictor of device thrombosis, while the device type was not associated with this outcome (p = 0.21). The higher incidence of events in patients receiving hybrid stenting reflects the higher complexity of the lesions in these patients; in patients treated with a hybrid strategy, the type of device implanted did not influence patients´ outcomes.
Volatile Anesthetics Rapidly Increase Dendritic Spine Density in the Rat Medial Prefrontal Cortex during Synaptogenesis

Background:Recent experimental observations suggest that, in addition to induce neuroapoptosis, anesthetics can also interfere with synaptogenesis during brain development. The aim of this study was to pursue this issue by evaluating the exposure time-dependent effects of volatile anesthetics on neuronal cytoarchitecture in 16-day-old rats, a developmental stage characterized by intense synaptogenesis in the cerebral cortex. Methods:Whistar rats underwent isoflurane (1.5%), sevoflurane (2.5%), or desflurane (7%) anesthesia for 30, 60, and 120 min at postnatal day 16, and the effect of these treatments on neuronal cytoarchitecture was evaluated 6 h after the initiation of anesthesia. Cell death was assessed using Fluoro-Jade B staining and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling assay. Ionotophoretic injections into layer 5 pyramidal neurons in the medial prefrontal cortex allowed visualization of dendritic arbor. Tracing of dendritic tree was carried out using the Neurolucida station (Microbrightfield, Williston, VT), whereas dendritic spines were analyzed using confocal microscopy. Results:Up to a 2-h-long exposure, none of the volatile drugs induced neuronal cell death or significant changes in gross dendritic arbor pattern of layer 5 pyramidal neurons in pups at postnatal day 16. In contrast, these drugs significantly increased dendritic spine density on dendritic shafts of these cells. Importantly, considerable differences were found between these three volatile agents in terms of exposure time-dependent effects on dendritic spine density. Conclusion:These new results suggest that volatile anesthetics, with different potencies and without inducing cell death, could rapidly interfere with physiologic patterns of synaptogenesis and thus might impair appropriate circuit assembly in the developing cerebral cortex.
The Multiple Faces of C-Reactive Protein—Physiological and Pathophysiological Implications in Cardiovascular Disease

C-reactive protein (CRP) is an intriguing protein which plays a variety of roles in either physiological or pathophysiological states. For years it has been regarded merely as a useful biomarker of infection, tissue injury and inflammation, and it was only in the early 80s that the modified isoforms (mCRP) of native CRP (nCRP) appeared. It soon became clear that the roles of native CRP should be clearly discriminated from those of the modified form and so the impacts of both isoforms were divided to a certain degree between physiological and pathophysiological states. For decades, CRP has been regarded only as a hallmark of inflammation; however, it has since been recognised as a significant predictor of future episodes of cardiovascular disease, independent of other risk factors. The existence of modified CRP isoforms and their possible relevance to various pathophysiological conditions, suggested over thirty years ago, has prompted the search for structural and functional dissimilarities between the pentameric nCRP and monomeric mCRP isoforms. New attempts to identify the possible relevance between the diversity of structures and their opposing functions have initiated a new era of research on C-reactive protein. This review discusses the biochemical aspects of CRP physiology, emphasizing the supposed relevance between the structural biology of CRP isoforms and their differentiated physiological and pathophysiological roles.
Depression in patients with epilepsy in Northwestern Nigeria: Prevalence and clinical correlates

Background: The impact of seizure disorder on people living with epilepsy (PWE) is worsened by the occurrence of comorbid psychiatric disorders, such as depression, which have been found commonly in PWE. Despite the dire consequences comorbid depression has on PWE, it still remains underdiagnosed and undertreated. Objective: To determine the prevalence of depression and associated clinical factors in PWE in Northwestern Nigeria. Materials and Methods: A total of 255 consecutive patients with epilepsy aged 18 years and above, from two health facilities, were recruited in this cross-sectional study. Following completion of a structured proforma detailing sociodemographic and seizure characteristics, Mini International Neuropsychiatric Interview was administered to diagnose depression in the patients. Results: A total of 255 patients, with a mean age of 32 years (standard deviation = 1.31), comprising 147 (57.6%) males and 108 (42.4%) females were studied. Majority (79.2%) of the patients had primarily generalized seizure type. Overall, depressive disorder was present in 52 (20.4%) patients. A significant association was found between previous hospitalization for epilepsy (P = 0.009), increased frequency of seizures, (P = 0.004), and prolonged duration of epilepsy, (P = 0.006). The independent predictors of depression included duration of epilepsy (P = 0.0001), previous hospitalization for epilepsy (P = 0.011), and frequency of seizures (P = 0.028). Conclusion: Depression was common in PWE. Female gender, previous hospitalization for epilepsy, increased frequency of seizures and prolonged duration of epilepsy were associated with depression in PWE. Previous hospitalization for epilepsy, increased frequency of seizures, and prolonged duration of epilepsy were independent predictors of depression.
Induced expression of PD‐1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death.

The classical type of programmed cell death is characterized by its dependence on de novo RNA and protein synthesis and morphological features of apoptosis. We confirmed that stimulated 2B4.11 (a murine T‐cell hybridoma) and interleukin‐3 (IL‐3)‐deprived LyD9 (a murine haematopoietic progenitor cell line) died by the classical type of programmed cell death. Assuming that common biochemical pathways might be involved in the deaths of 2B4.11 and LyD9, we isolated the PD‐1 gene, a novel member of the immunoglobulin gene superfamily, by using subtractive hybridization technique. The predicted PD‐1 protein has a variant form of the consensus sequence found in cytoplasmic tails of signal transducing polypeptides associated with immune recognition receptors. The PD‐1 gene was activated in both stimulated 2B4.11 and IL‐3‐deprived LyD9 cells, but not in other death‐induced cell lines that did not show the characteristic features of the classical programmed cell death. Expression of the PD‐1 mRNA in mouse was restricted to the thymus and increased when thymocyte death was augmented by in vivo injection of anti‐CD3 antibody. These results suggest that activation of the PD‐1 gene may be involved in the classical type of programmed cell death.
Doped Layer Optimization for Silicon Heterojunctions by Injection-Level-Dependent Open-Circuit Voltage Measurements

Besides passivation of the c-Si absorber, provided mainly by the undoped buffer layer, the net doping of the silicon thin films plays a major role in the performance of silicon-based heterojunction (SHJ) solar cells. However, junction engineering is complex as high net doping often interferes with the interface passivation and the optical properties of the silicon thin films. We show that injection-level-dependent open-circuit voltage (Suns- Voc) measurements are a simple and valuable method for the characterization and optimization of the doped amorphous silicon (a-Si:H) layers. It is shown by experiment and device simulations that at high illumination intensities the Suns- Voc characteristic exhibits a strong signature of defect recombination within the a-Si:H, which is determined by the a-Si:H doping and the interfacial transparent conducting oxide (TCO) properties. This fact is exploited for a qualitative interpretation of the interplay between a-Si:H and the interfacial TCO properties. As a clear correlation between the Suns- Voc characteristic and the maximum power point conditions of the devices exists, fill factor (FF) losses attributed to the doped a-Si:H and the interfacial TCO properties can 1) be easily predicted in the early stage of device optimization on simple test structures, or 2) these FF losses can be identified and distinguished from other FF losses in the final device.
The Ca-loop in thymidylate kinase is critical for growth and contributes to pyrimidine drug sensitivity of Candida albicans

The yeast Candida albicans is the most prevalent opportunistic fungal pathogen in humans. Drug resistance among C. albicans isolates poses a common challenge, and overcoming this resistance represents an unmet need in managing this common pathogen. Here, we investigated CDC8, encoding thymidylate kinase (TMPK), as a potential drug target for the management of C. albicans infections. We found that the region spanning amino acids 106–123, namely the Ca-loop of C. albicans TMPK (CaTMPK), contributes to the hyperactivity of this enzyme compared with the human enzyme (hTMPK) and to the utilization of deoxyuridine monophosphate (dUMP)/deoxy-5-fluorouridine monophosphate (5-FdUMP) as a substrate. Notably, expression of CaTMPK, but not of hTMPK, produced dUTP/5-FdUTP–mediated DNA toxicity in budding yeast (Saccharomyces cerevisiae). CRISPR-mediated deletion of this Ca-loop in C. albicans revealed that the Ca-loop is critical for fungal growth and susceptibility to 5-fluorouridine (5-FUrd). Of note, pathogenic and drug-resistant C. albicans clones were similarly sensitive to 5-FUrd, and we also found that CaTMPK is essential for the growth of C. albicans. In conclusion, these findings not only identified a target site for the development of CaTMPK-selective drugs, but also revealed that 5-FUrd may have potential utility as drug for managing C. albicans infections.