Miracle-dz/UniFAR-FTUs huggingface dataset:
Contrasting Histopathology and Crystal Deposits in Kidneys of Idiopathic Stone Formers Who Produce Hydroxy Apatite, Brushite, or Calcium Oxalate Stones

Our previous work has shown that stone formers who form calcium phosphate (CaP) stones that contain any brushite (BRSF) have a distinctive renal histopathology and surgical anatomy when compared with idiopathic calcium oxalate stone formers (ICSF). Here we report on another group of idiopathic CaP stone formers, those forming stone containing primarily hydroxyapatite, in order to clarify in what ways their pathology differs from BRSF and ICSF. Eleven hydroxyapatite stone formers (HASF) (2 males, 9 females) were studied using intra‐operative digital photography and biopsy of papillary and cortical regions to measure tissue changes associated with stone formation. Our main finding is that HASF and BRSF differ significantly from each other and that both differ greatly from ICSF. Both BRSF and ICSF patients have significant levels of Randall's plaque compared with HASF. Intra‐tubular deposit number is greater in HASF than BRSF and nonexistent in ICSF while deposit size is smaller in HASF than BRSF. Cortical pathology is distinctly greater in BRSF than HASF. Four attached stones were observed in HASF, three in 25 BRSF and 5–10 per ICSF patient. HASF and BRSF differ clinically in that both have higher average urine pH, supersaturation of CaP, and calcium excretion than ICSF. Our work suggests that HASF and BRSF are two distinct and separate diseases and both differ greatly from ICSF. Anat Rec, 297:731–748, 2014. © 2014 Wiley Periodicals, Inc.
Motivations of educational tourists in non-English-speaking countries: the role of languages

ABSTRACT Learning a language or improving one’s foreign language skills may be a key motivator for students to decide to participate in an exchange program as well as choose the destination for their studies. Nevertheless, studies on educational tourism are limited, and there is no research on the motivations of students visiting non-English-speaking countries. To fill this gap, this paper aims to examine the motivations of university students in non-English-speaking destinations by using the push-and-pull framework. Primary data were collected through an online survey from 190 and 205 students who participated in mobility programs in a Spanish university and in a German university respectively. Empirical findings reveal that languages are a key motivating factor for either growing personally through cultural enrichment, or growing professionally. Furthermore, these data allow generic recommendations so that the destinations can attract a larger number of international students.
Spatially scaled motion-reproduction control using modified motion data

Modifying data of audio and visual information makes sound from speaker or display size larger or smaller. However, motion data has a different characteristic from audio and visual data in that it is a bilateral information. When the movement of motion data is scaled, it is affected by reaction force from environments. In this paper, the spatially scaled motion data is handled. In the conventional method of motion-copying system, the stored motion is reproduced only when environmental conditions of stored and reproduced phases are same. So, when a motion data is modified, the movement of motion data is changed and reaction force from environments is also changed. Therefore, the change of the different environmental condition affects the control system and the desired modified motion is not reproduced. In this paper, the scaled motion-reproduction control using spatially scaled motion data based on position-based motion-copying system is proposed. The validity of the proposed method is verified by simulations and experiments and the proposed method is effective for size-free motion reproduction and data volume reduction.
Airport Capacity Allocation with Network Airlines

This study provides a theoretical framework to investigate the efficiency of different airport capacity allocation schemes under congestion externalities and imperfect competition. Its innovation is to consider an asymmetric market structure featuring a single dominant network carrier at its hub airport that endogenously differentiates its flights based on passenger benefits from network density. Based on a partial equilibrium analysis of the generic model, the investigation qualitatively evaluates the ambiguities on allocation efficiency, which arise with capacity allocation in a market that faces multiple distortions. In addition, a quantitative simulation comprehensively illustrates the market structures of the model, as well as selected outcomes concerning the allocation instruments. At the end, a brief summary helps to quickly access the main lessons of the extensive investigation; moreover, the conclusions are extended into implications for practice, which may be interesting for regulators, policymakers, and airline managers likewise.
Developing genome and exome sequencing for candidate gene identification in inherited disorders: an integrated technical and bioinformatics approach.

CONTEXT Advances in sequencing technology with the commercialization of next-generation sequencing (NGS) has substantially increased the feasibility of sequencing human genomes and exomes. Next-generation sequencing has been successfully applied to the discovery of disease-causing genes in rare, inherited disorders. By necessity, the advent of NGS has fostered the concurrent development of bioinformatics approaches to expeditiously analyze the large data sets generated. Next-generation sequencing has been used for important discoveries in the research setting and is now being implemented into the clinical diagnostic arena. OBJECTIVE To review the current literature on technical and bioinformatics approaches for exome and genome sequencing and highlight examples of successful disease gene discovery in inherited disorders. To discuss the challenges for implementing NGS in the clinical research and diagnostic arenas. DATA SOURCES Literature review and authors' experience. CONCLUSIONS Next-generation sequencing approaches are powerful and require an investment in infrastructure and personnel expertise for effective use; however, the potential for improvement of patient care through faster and more accurate molecular diagnoses is high.
Regulation of cytochromes P450 during inflammation and infection.

Hepatic P450 activities are profoundly affected by various infectious and inflammatory stimuli, and this has clinical and toxicological consequences. Whereas the expression of most P450s in the liver is suppressed, some are induced. Many of the effects observed in vivo can be mimicked by pro-inflammatory cytokines and IFNs, and P450s are differentially regulated by these agents. Therefore, different cytokine profiles and concentrations in the vicinity of the hepatocyte in different models of inflammation may result in qualitatively and quantitatively different effects on populations of P450s. In addition to cytokines, glucocorticoids may have an important role in P450 regulation in stress conditions, including that caused by inflammatory stimuli. Although in many cases the decreases in activity are due primarily to a down-regulation of P450 gene transcription, it is likely that modulation of RNA and protein turnover, as well as enzyme inhibition, contributes to some of the observed effects. The mechanisms whereby these effects are produced may also vary with both the P450 under study and the time course of the effect. The complexity of the P450 response to inflammation and infection means that all of the above factors must be considered when trying to predict the effect of a given infectious or inflammatory condition on the clinical or toxic response of humans or animals to an administered drug or toxin. The question of whether the down-regulation of the hepatic P450 system to inflammation or infection is a homeostatic or pathological response cannot be answered at present. It is difficult to discern the physiological benefit of reducing hepatic P450 activities, unless it is to prevent the generation of reactive oxygen species generated by uncoupled catalytic turnover of the enzymes. On the other hand, as we proposed some years ago [64], the suppression of P450 may be due to the liver's need to utilize its transcriptional machinery and energy for the synthesis of APPs involved in the inflammatory response. In that case, one could ask why the organism has gone to the trouble of employing differential mechanisms for suppression of P450. One answer could be that the response evolved after the divergence of many of the P450 genes, necessitating the evolution of multiple redundant mechanisms for P450 suppression. In contrast to the down-regulation of P450s in the liver, the induction of several forms in this and other tissues suggests a more specific homeostatic role of these effects, e.g., in generation or catabolism of bioactive metabolites.
Migraine With Aura and Migraine Without Aura: An Epidemiological Study

In a cross-sectional study of headache disorders in a representative general population of 1,000 persons the epidemiology of migraine with aura (MA) and migraine without aura (MO) was analysed in relation to sex and age distribution, symptomatology and precipitants. The headache disorders were classified on the basis of a clinical interview as well as a physical and a neurological examination using the operational diagnostic criteria of the International Headache Society (IHS). Lifetime prevalence of MA was 5%, male:female ratio 1:2. Lifetime prevalence of MO was 8%, M:F ratio 1:7. Women, but not men, were significantly more likely to have MO than MA. Neither MA nor MO showed correlation to age in the studied age interval (25–64 years). Premonitory symptoms occurred in 16% of subjects with MA and in 12% with MO. One or more precipitating factor was present in 61% with MA and in 90% with MO. In both MA and MO the most conspicuous precipitating factor was stress and mental tension. Visual disturbances were the most common aura phenomenon occurring in 90% of subjects with MA. Aura symptoms of sensory, motor or speech disturbances rarely occurred without coexisting visual disturbances. The pain phase of MA fulfilled the criteria for MO of the IHS. Headache was, however, less severe and shorter lasting in MA than in MO. Onset at menarche, menstrual precipitation, menstrual problems, influence of pregnancy and use of oral contraceptives all showed some relationship with the presence of MO and less with MA. The present findings suggest that MA and MO share the pain phase. Among subjects with MA and MO, 50% and 62%, respectively, had consulted their general practitioner because of migraine. Selection bias in previous clinical studies is demonstrated by comparisons with the present unselected sample.
Emerging therapies for portal hypertension in cirrhosis

ABSTRACT Introduction: Counteracting splanchnic vasodilatation and increased portal-collateral blood flow has been the mainstay for the treatment of portal hypertension (PH) over the past three decades. However, there is still large room for improvement in the treatment of PH. Areas covered: The basic mechanism leading to portal hypertension is the increased hepatic vascular resistance to portal blood flow caused by liver structural abnormalities inherent to cirrhosis and increased hepatic vascular tone. Molecules modulating microvascular dysfunction which have undergone preclinical and clinical trials are summarized, potential drug development issues are addressed, and situations relevant to design of clinical trials are considered. Expert opinion: Experimental and clinical evidence indicates that molecules modulating liver microvascular dysfunction may allow for 30–40% reduction in portal pressure. Several agents could be utilized in the earlier stages of cirrhosis (antifibrotics, antiangiogenics, etiological therapies) may allow reduction of fibrosis and halt progression of PH. This ‘nip at the bud’ policy, by combining therapies with existing agents used in advanced phase of cirrhosis and novel agents which could be used in early phase of cirrhotic spectrum, which are likely to hit the market soon would be the future strategy for PH therapy.
Proteins of Newly Isolated Mutants and the Amino-terminal Proline Are Essential for Ubiquitin-Proteasome-catalyzed Catabolite Degradation of Fructose-1,6-bisphosphatase of Saccharomyces cerevisiae *

Addition of glucose to cells of the yeastSaccharomyces cerevisiae growing on a non-fermentable carbon source leads to selective and rapid degradation of fructose-1,6-bisphosphatase. This so called catabolite inactivation of the enzyme is brought about by the ubiquitin-proteasome system. To identify additional components of the catabolite inactivation machinery, we isolated three mutant strains, gid1,gid2, and gid3, defective in glucose-induced degradation of fructose-1,6-bisphospha-tase. All mutant strains show in addition a defect in catabolite inactivation of three other gluconeogenic enzymes: cytosolic malate dehydrogenase, isocitrate lyase, and phosphoenolpyruvate carboxykinase. These findings indicate a common mechanism for the inactivation of all four enzymes. The mutants were also impaired in degradation of short-lived N-end rule substrates, which are degraded via the ubiquitin-proteasome system. Site-directed mutagenesis of the amino-terminal proline residue yielded fructose-1,6-bisphosphatase forms that were no longer degraded via the ubiquitin-proteasome pathway. All amino termini other than proline made fructose-1,6-bisphosphatase inaccessible to degradation. However, the exchange of the amino-terminal proline had no effect on the phosphorylation of the mutated enzyme. Our findings suggest an essential function of the amino-terminal proline residue for the degradation process of fructose-1,6-bisphosphatase. Phosphorylation of the enzyme was not necessary for degradation to occur.
Differential passivity like properties for a class of nonlinear systems

In this paper we derive new passive maps akin to incremental passive maps, for a class of nonlinear systems using dynamic feedback and Krasovskii's method. Further using the passive maps we present a control methodology for stabilization to a desired operating point. This work is illustrated by designing a controller for a nonlinear building heating ventilating and air conditioning (HVAC) subsystem.
Molecular architecture of the vesicular stomatitis virus RNA polymerase

Nonsegmented negative-strand (NNS) RNA viruses initiate infection by delivering into the host cell a highly specialized RNA synthesis machine comprising the genomic RNA completely encapsidated by the viral nucleocapsid protein and associated with the viral polymerase. The catalytic core of this protein–RNA complex is a 250-kDa multifunctional large (L) polymerase protein that contains enzymatic activities for nucleotide polymerization as well as for each step of mRNA cap formation. Working with vesicular stomatitis virus (VSV), a prototype of NNS RNA viruses, we used negative stain electron microscopy (EM) to obtain a molecular view of L, alone and in complex with the viral phosphoprotein (P) cofactor. EM analysis, combined with proteolytic digestion and deletion mapping, revealed the organization of L into a ring domain containing the RNA polymerase and an appendage of three globular domains containing the cap-forming activities. The capping enzyme maps to a globular domain, which is juxtaposed to the ring, and the cap methyltransferase maps to a more distal and flexibly connected globule. Upon P binding, L undergoes a significant rearrangement that may reflect an optimal positioning of its functional domains for transcription. The structural map of L provides new insights into the interrelationship of its various domains, and their rearrangement on P binding that is likely important for RNA synthesis. Because the arrangement of conserved regions involved in catalysis is homologous, the structural insights obtained for VSV L likely extend to all NNS RNA viruses.
Decreased epidermal growth factor binding in cells growth arrested in G1 by nutrient deficiency

Previous studies have shown that the nontransformed AKR‐2B mouse embryo derived cell line may growth arrest by two separate mechanisms in the G1 phase of the cell cycle‐growth factor deficiency arrest (G0) and low molecular weight nutrient deficiency arrest. An examination of epidermal growth factor (EGF) receptors under the different resting or growth conditions has shown that rapidly growing cells or cells arrested due to growth factor deficiency have the expected amount of 125I‐EGF binding with approximately 105 receptors per cell being present in G0 arrested cells. In contrast, cells arrested due to nutrient deficiency show a reduction in 125I‐EGF binding to 10‐‐20% of that observed under the other conditions. This effect appears to be due to decreased receptor number and not to a change in the affinity of the receptor. Stimulation of DNA synthesis by nutrient replenishment causes a tenfold increase in EGF binding 20 hours later, with some increase in binding being detectable as early as six hours. The increase in binding is inhibited by cycloheximide and actinomycin D. This suggests that new mRNA synthesis as well as increased protein synthesis is required for the increase in EGF binding.
The relationship of HIV prevalence in pregnant women to that in women of reproductive age: a validated method for adjustment

Objective:To devise and validate a method for adjusting HIV seroprevalences in pregnant women to estimate population prevalences among all women in their child-bearing years. Design:Birth and termination rates from women with known HIV infection in the United Kingdom were calculated according to the likely route of HIV infection and whether HIV infection was diagnosed. Methods:Birth and termination rates were weighted and combined to produce summary statistics. Comparisons were then made with population birth and termination rates to derive summary relative inclusion ratios (RIRs), the relative probabilities of including HIV-infected and uninfected women in seroprevalence surveys of pregnant women. Results:The derived RIRs for women having live births were close to unity: 1.03 [95% confidence intervals (CI) 0.90–1.17] for London and 0.80 (CI, 0.71–0.89) for elsewhere in England and Wales. This indicates that currently observed overall seroprevalences among pregnant women having live births in London would be similar to those among all women of the same age, while elsewhere it would be slightly underestimated. Sensitivity analysis indicated that RIRs could, however, vary three-fold (0.47–1.56) according to the proportion of diagnosed maternal infections and the mix of maternal HIV-exposure categories. The method was validated by using it to predict the ratio of unlinked seroprevalences between women having terminations and live births in London. It predicted a ratio of 1.74 : 1, which is close to the observed ratio of 2.07 : 1. Conclusions:Application of HIV seroprevalences from pregnant women to whole populations may need adjustment for fertility rates among HIV-infected women. A general method for this has been derived and validated. Gathering fertility data for HIV-infected women is a useful adjunct to serosurveillance.
Clonal Multipotency of Skeletal Muscle‐Derived Stem Cells Between Mesodermal and Ectodermal Lineage

The differentiation potential of skeletal muscle‐derived stem cells (MDSCs) after in vitro culture and in vivo transplantation has been extensively studied. However, the clonal multipotency of MDSCs has yet to be fully determined. Here, we show that single skeletal muscle‐derived CD34−/CD45− (skeletal muscle‐derived double negative [Sk‐DN]) cells exhibit clonal multipotency that can give rise to myogenic, vasculogenic, and neural cell lineages after in vivo single cell‐derived single sphere implantation and in vitro clonal single cell culture. Muscles from green fluorescent protein (GFP) transgenic mice were enzymatically dissociated and sorted based on CD34 and CD45. Sk‐DN cells were clone‐sorted into a 96‐well plate and were cultured in collagen‐based medium with basic fibroblast growth factor and epidermal growth factor for 14 days. Individual colony‐forming units (CFUs) were then transplanted directly into severely damaged muscle together with 1 × 105 competitive carrier Sk‐DN cells obtained from wild‐type mice muscle expanded for 5 days under the same culture conditions using 35‐mm culture dishes. Four weeks after transplantation, implanted GFP+ cells demonstrated differentiation into endothelial, vascular smooth muscle, skeletal muscle, and neural cell (Schwann cell) lineages. This multipotency was also confirmed by expression of mRNA markers for myogenic (MyoD, myf5), neural (Musashi‐1, Nestin, neural cell adhesion molecule‐1, peripheral myelin protein‐22, Nucleostemin), and vascular (α‐smooth muscle actin, smoothelin, vascular endothelial‐cadherin, tyrosine kinase‐endothelial) stem cells by clonal (single‐cell derived) single‐sphere reverse transcription‐polymerase chain reaction. Approximately 70% of clonal CFUs exhibited expression of all three cell lineages. These findings support the notion that Sk‐DN cells are a useful tool for damaged muscle‐related tissue reconstitution by synchronized vasculogenesis, myogenesis, and neurogenesis.
Stock Liquidity and Firm Innovation: A Comparative Study Based on the Perspective of Firm Size

At present, there are serious differences in the study of the impact of stock liquidity on enterprise innovation. Taking A share listed companies as the research object, this paper classifies the relationship between stock liquidity and enterprise innovation from the perspective of enterprise size. The empirical study finds that the liquidity of the large enterprises in the A stock market will promote the innovation of the enterprises, while the small and medium enterprises are hindered. Finally, suggestions are made from the supervision and legislation of the information disclosure, the rational release of liquidity and the increase of the proportion of institutional investors.
Stability Measurements in Multifilamentary ${\rm MgB}_{2}$ Tapes

We have analyzed the behavior of a 10 cm long straight piece of copper stabilized multifilamentary tape, manufactured by Columbus Superconductors, in a nearly adiabatic condition. The tape was biased at different current values as a function of either the temperature or the induced heat disturbances at fixed temperatures. From the analyses of the voltage increase, recorded from voltage taps placed at known positions, we estimated the highest temperatures which give rise to a quench of the tape at constant values of the current bias. We also measured the minimum energy triggering the quench at fixed values of current bias and temperature. The voltage taps have also been used for homogeneity check of the physical properties which rule the thermal runaway in the tape. The voltage-versus-time curves in different sections of the tape also provide information about the quench propagation speed as a function of the hot spot energy and the temperature. Due to the composite nature of the tape, made of nickel, iron, copper, and , to perform simple FEA computation we modeled the tape with a one dimensional single wire with averaged value of the physical quantities involved in the Joule heat generation and thermal conductivity.