PRODUCTION AND CHARACTERIZATION OF NANOPARTICLES LIPID CARRIER (NLCS) LOADED WITH GINGEROLS EXTRACT AND THEIR EFFECT ON SERUM LIPID PROFILE IN POSTMENOPAUSAL PERIOD

Plant-derived edible nanoparticles (PDNPs) are nano-sized membrane vesicles released by edible plants. They are non-toxic, have tissue-specific targeting properties, and can be mass-produced. The study aimed to assess the effect of gingerols to decreased the levels of total cholesterol (TC), triglycerides (TG) and lipoproteins (HDL, LDL, VLDL) levels that associated with menopausal transition using nanoparticles lipid carrier (NLCs) loaded with gingerols extract (GE-NLCs). The study was conducted using thirty adult female mice divided into three groups (10 mice/ group) and handled as follows for 6 weeks. Group A: Control group has injected 0.1 ml D.W intraperitoneal (IP) daily. Group B: This group has injected IP daily 160 mg/ kg B.W of 4-Vinylcyclohexene dioxide (VCD) for two weeks as model for menopause, group C: Animals in this group were administered 160 mg /kg B.W of VCD IP daily for two weeks for inducing menopause, then shaved at the last third of the back and treated with dermal sticker saturated with 0.1ml of GE-NLCs for six weeks. A gingerols extract standardized by Highperformance liquid chromatography (HPLC) to contain (18.3) mg/g of gingerols. The characterization of GE-NLCs was done by transmission electron microscopy (TEM), Scanning probe microscopy (SPM), Zeta potential. TEM images showed that shape of particles was mostly spherical and few cylindrical with average diameter (64.31) nm. SPM images showed the average grain size was (42.81) nm. Encapsulation Efficiency (EE) was 85%. Zeta potential was (-42.9) Mv. The results of statistical analysis showed a significant decrease (P0.01) in the level of estrogen in group B (23.767) ng/ml, compared with group A (33.154) ng/ml and significant increase in the estrogen level in group C (29.742) ng/ml compared with group B. While for levels of lipids, the result of statistical analysis showed a significant increase in the level of TC in group B (101.94) mg/dl, compared with group A (80.00) mg / dl, and significant decreased in group C (80.86) mg/dl, compared with group B and significant increase (P0.01) in the level of TG in group B (79.400) mg/dl, compared with group A (54.200) mg/dl and significant decrease in group C compared (61.158) mg/dl, compared with group B. and The result s of statistical analysis showed a significant decreased (P0.01) in the level of HDL in group B (18.800) mg/dl, compared with group A (23.001) mg/dl and no significant difference in group C (18.168) mg/dl, compared with group B and significant increase in the level of LDL in group B (66.88) mg/dl, compared with group A(50.36) mg/dl and significant decreased in group C (44.71) mg/dl, compared with group B and results of statistical analysis showed a significant increased (P<0.01) in the level of VLDL in group B (16.62) mg/dl, compared with group A (10.84) mg/dl and significant decreased in group C (11.90) mg/dl, compared with group B. Conclusion: The results of this study revealed that administration of GE-NLCs shows an effective to regulation of lipids metabolism disorders due to hormonal changes that associated with menopausal transition via impact of VCD.