Miracle-dz/UniFAR-FTUs huggingface dataset:
Mycoplasma pneumoniae from the Respiratory Tract and Beyond

SUMMARY Mycoplasma pneumoniae is an important cause of respiratory tract infections in children as well as adults that can range in severity from mild to life-threatening. Over the past several years there has been much new information published concerning infections caused by this organism. New molecular-based tests for M. pneumoniae detection are now commercially available in the United States, and advances in molecular typing systems have enhanced understanding of the epidemiology of infections. More strains have had their entire genome sequences published, providing additional insights into pathogenic mechanisms. Clinically significant acquired macrolide resistance has emerged worldwide and is now complicating treatment. In vitro susceptibility testing methods have been standardized, and several new drugs that may be effective against this organism are undergoing development. This review focuses on the many new developments that have occurred over the past several years that enhance our understanding of this microbe, which is among the smallest bacterial pathogens but one of great clinical importance.
CRYSTAL-INDUCED INFLAMMATION IN CANINE JOINTS

In an experimental model, the polymorphonuclear leukocyte was found to be necessary to the inflammation induced by crystals. This conclusion is based on (a) the invariable migration of polymorphonuclear leukocytes into the synovial fluid of canine joints injected with urate crystals, (b) the experimental suppression of the synovitis by the depletion of polymorphonuclear leukocytes in animals treated with vinblastine, (c) the dependence of the degree of suppression on the degree of leukocyte mobilization into the joint space, and (d) the restoration of the inflammatory response in a leukopenic animal by perfusion with normal blood.
Malignant extrarenal rhabdoid tumor: a case report

Abstract The fetal extrarenal rhabdoid tumor is a highly aggressive and rare neoplasm that mostly occurs in the first year of life. In this case, a remarkably enlarged right upper arm was diagnosed in a male fetus at 31+2 weeks of gestation. Because of a fast progressive tumor volume and signs of fetal distress, a cesarean section was performed at 34 weeks of gestation. The preterm baby showed a blue livid tumor reaching from the right elbow up to the shoulder that resulted in an extrarenal rhabdoid tumor on histopathologic analysis. A therapeutic trial with cyclophosphamide was not effective and the child died at the age of 26 days because of multi-organ failure.
Laboratory and Clinical Aspects of Human Herpesvirus 6 Infections

SUMMARY Human herpesvirus 6 (HHV-6) is a widespread betaherpesvirus which is genetically related to human cytomegalovirus (HCMV) and now encompasses two different species: HHV-6A and HHV-6B. HHV-6 exhibits a wide cell tropism in vivo and, like other herpesviruses, induces a lifelong latent infection in humans. As a noticeable difference with respect to other human herpesviruses, genomic HHV-6 DNA is covalently integrated into the subtelomeric region of cell chromosomes (ciHHV-6) in about 1% of the general population. Although it is infrequent, this may be a confounding factor for the diagnosis of active viral infection. The diagnosis of HHV-6 infection is performed by both serologic and direct methods. The most prominent technique is the quantification of viral DNA in blood, other body fluids, and organs by means of real-time PCR. Many active HHV-6 infections, corresponding to primary infections, reactivations, or exogenous reinfections, are asymptomatic. However, the virus may be the cause of serious diseases, particularly in immunocompromised individuals. As emblematic examples of HHV-6 pathogenicity, exanthema subitum, a benign disease of infancy, is associated with primary infection, whereas further virus reactivations can induce severe encephalitis cases, particularly in hematopoietic stem cell transplant recipients. Generally speaking, the formal demonstration of the causative role of HHV-6 in many acute and chronic human diseases is difficult due to the ubiquitous nature of the virus, chronicity of infection, existence of two distinct species, and limitations of current investigational tools. The antiviral compounds ganciclovir, foscarnet, and cidofovir are effective against active HHV-6 infections, but the indications for treatment, as well as the conditions of drug administration, are not formally approved to date. There are still numerous pending questions about HHV-6 which should stimulate future research works on the pathophysiology, diagnosis, and therapy of this remarkable human virus.
Richter's syndrome – update on biology and management

Introduction: Richter's syndrome (RS) refers to a histological transformation of chronic lymphocytic leukemia (CLL) into an aggressive lymphoma, most commonly diffuse large B-cell lymphoma (DLBCL) and rarely Hodgkin's lymphoma. Patients with RS can present with rapidly enlarging lymph nodes, worsening ‘B’ symptoms, progressive organomegaly and elevated serum lactate dehydrogenase, erythrocyte sedimentation rate and β2-microglobulin levels. Recently, it was demonstrated that RS has molecular and cytogenetic characteristics distinct from de novo DLBCL and untransformed CLL. Some of the predisposing factors for RS include use of prior multiple chemotherapies for CLL, the presence of CDKN2A, TP53 mutations, NOTCH1 mutations, c-MYC abnormalities, unmutated immunoglobulin heavy chain, non-del13q cytogenetics, CD38 gene polymorphisms, stereotypy of subset 8 of B-cell receptor (BCR) and VH 4 – 39 gene usage. Patients with RS have a poor prognosis (median survival 1 – 2 years). These patients achieve short lasting responses to chemoimmunotherapy (rituximab combined with chemotherapy). Stem-cell transplantation is beneficial for patients who achieve remission after first therapy. Further understanding of the molecular pathogenesis of RS may identify newer therapeutic targets. Areas covered: This article focuses on the treatment options for patients with RS. A thorough literature search has been undertaken and only the most relevant references are included. Expert opinion: Clear understanding of pathogenesis of RS and effective treatment of RS remains a challenge. Since there is no standard-of-care treatment for patients with RS, all the patients should preferentially enroll into clinical trials.
A Translational Research Evaluation of the Stay Active and Independent for Life (SAIL) Community-Based Fall Prevention Exercise and Education Program

Falls in older adults are the leading cause of injury hospitalizations and fatalities in the United States; primary risk factors are muscle weakness, impaired mobility, and balance deficits. This article describes the 12-month translational research evaluation of the Stay Active and Independent for Life (SAIL) community-based public health, public domain fall prevention exercise and education program. Recruitment reached the target goal by 154%; 331 adults (mean age = 74.6) attended more than one class (mean classes attended = 24.8, SD = 26.6, range = 1-120) at nine community sites in one county in the 12-month period; 173 completed health and demographic forms, 132 completed program surveys, and 91 completed baseline and follow-up physical function tests. Physical function test results showed significant improvements in strength, balance, and mobility in those who were below normal limits at baseline, and in those who attended classes twice a week or more for more than 2 months. Survey results found that 93% of respondents reported improved performance of daily activities; 92% reported improved strength, balance, fitness, or flexibility; and 80% found the SAIL information guide education component helpful.
Looking into Task-Specific Activation Using a Prosthesis Substituting Vision with Audition

A visual-to-auditory sensory substitution device initially developed for the blind is known to allow visual-like perception through sequential exploratory strategies. Here we used functional magnetic resonance imaging (fMRI) to test whether processing the location versus the orientation of simple (elementary) “visual” stimuli encoded into sounds using the device modulates the brain activity within the dorsal visual stream in the absence of sequential exploration of these stimuli. Location and orientation detection with the device induced a similar recruitment of frontoparietal brain areas in blindfolded sighted subjects as the corresponding tasks using the same stimuli in the same subjects in vision. We observed a similar preference of the right superior parietal lobule for spatial localization over orientation processing in both sensory modalities. This provides evidence that the parietal cortex activation during the use of the prosthesis is task related and further indicates the multisensory recruitment of the dorsal visual pathway in spatial processing.
Exposure to Multiple Components of a Garden-Based Intervention for Middle School Students Increases Fruit and Vegetable Consumption

Objectives. (1) To measure the effects of different levels of exposure to a multiple-component garden-based intervention on middle school students’ fruit and vegetable (F&V) consumption and related variables and (2) to determine the separate effects of each of the intervention components on F&V consumption. Design. Unequal treatment–control posttest only. Setting. Five middle schools in ethnically diverse communities. Participants. Two hundred and forty-six adolescents (59% Hispanic; 70% low-income). Intervention. The Sprouting Healthy Kids intervention consists of six components: (1) in-class lessons, (2) after-school gardening program, (3) farm-to-school, (4) farmers’ visits to schools, (5) taste testing, (6) field trips to farms. Outcome measures. F&V consumption; motivation for eating F&V; self-efficacy for eating F&V; F&V preference; preference for unhealthy foods; knowledge. Analysis. Linear regression models controlling for gender, ethnicity/race, and income. Results. Compared with students who were exposed to less than two intervention components, students who were exposed to two or more components scored significantly higher on F&V intake, self-efficacy, and knowledge and lower on preference for unhealthy foods (p < .05). The farmers’ visits, taste testing, and cafeteria components had the largest effect sizes but were not significant. Conclusions and implications. Interventions targeting adolescent F&V behavior change should include multiple components such as farmers’ visits to the school and taste testing.
Comparative Performance of a Novel Herpes Simplex Virus Type 2-Specific Enzyme-Linked Immunosorbent Assay Using a Targeted Chain Oligopeptide, Peptide 55

ABSTRACT Herpes simplex virus (HSV) glycoprotein G (gG2) has been used as the basis of many serological assays for the detection of HSV type 2 (HSV-2)-specific antibodies. In the present study, an enzyme-linked immunosorbent assay (ELISA), the Pathozyme Viro HSV-2 immunoglobulin G (IgG) ELISA (Omega Diagnostics, Alva, United Kingdom), based on an immunodominant epitope of gG2 presented in a branched-chain format (peptide 55), was compared with two commercially available gG2-specific assays, the Bioelisa HSV-2 IgG assay (Biokit, S.A., Barcelona, Spain) and the HerpesSelect HSV-2 IgG assay (Focus Diagnostics, Cypress, CA). A panel of 218 well-characterized serum samples was tested. Thirty-one samples were determined to be HSV-2 IgG antibody positive and 164 samples were determined to be negative with all three kits. The levels of concordance between the tests were 95.9% between the Omega and HerpeSelect assays, 90.8% between the Omega and Bioelisa assays, and 94.5% between the HerpeSelect and Bioelisa assays. Twenty-three samples gave discordant results. Western blot results showed that of these, the results for 77% were correctly identified by the Omega assay, the results for 68% were correctly identified by the HerpeSelect assay, and the results for 13.6% were correctly identified by the Bioelisa assay. Although there was a high level of agreement between the results obtained by the three assays and no false-positive results were detected by any of the three kits, confirmation of the results for samples with discordant results by Western blotting suggested that the peptide 55-based Omega assay is the most sensitive and specific assay among the assays evaluated.
Evaluation of the Avon Foundation Community Education and Outreach Initiative Community Patient Navigation Program

Introduction. Black women in the United States experience disproportionate breast cancer mortality. Culturally appropriate community education on the importance of breast health coupled with the availability of free or low-cost mammography screening services may help improve the use of mammography screening services among Black women. The Avon Foundation Community Patient Navigation Program seeks to fill this need. The current study presents a process and outcome evaluation of this program. Method. Trained and uniformed community patient navigators (PNs) host breast health education events where they recruit community members to complete a mammography interest form. Participants are referred to a nurse practitioner who determines eligibility for a free or low-cost mammogram. The community PN delivers telephone follow-up to encourage participants to make and keep their mammogram appointments. Results. Over a 15-month period, 22 community PNs hosted 207 breast health events, which included 9,601 attendees. Three hundred and four participants completed a mammography interest form, and 21% of these individuals received mammograms at the collaborating health facility. Participants who reported breast symptoms were twice as likely to get a mammogram as those who did not report symptoms. Discussion. Community patient navigation may be a useful resource for encouraging mammography screening among underserved women.
Parasitic Infections in Pregnancy Decrease Placental Transfer of Antipneumococcus Antibodies

ABSTRACT Many factors can influence maternal placental antibody transfer to the fetus, which confers important immune protection to the newborn infant. However, little is known about the effect of maternal parasitic infection on placental antibody transfer. To investigate this, we selected from a parent study of 576 pregnant Kenyan women four groups of women with term deliveries (≥37 weeks), including uninfected women (n = 30) and women with solo infections with malaria (n = 30), hookworm (n = 30), or schistosomiasis (n = 10). Maternal plasma at delivery and infant cord blood were tested via multiplex fluorescent bead assay for IgG against 10 pneumococcal serotypes (PnPs 1, 4, 5, 6B, 7F, 9V, 14, 18C, 19F, and 23F), diphtheria toxoid, and Haemophilus influenzae type B. Infants born to mothers with prenatal malaria, hookworm, or Schistosoma haematobium infections were associated with a significantly reduced ratio of maternal to infant cord blood antibody concentration for Streptococcus pneumoniae serotypes 1, 4, 5, 6B, 7F, 9V, and 18C compared to infants of uninfected mothers. Anti-diphtheria toxoid and anti-H. influenzae type B IgG ratios were not significantly different among infection groups. Prenatal parasitic infections decrease the transfer of maternal IgG antibodies to infants for several serotypes of S. pneumoniae.
Challenges and Lessons Learned From the Translation of Evidence-Based Childhood Asthma Interventions

The Merck Childhood Asthma Network (MCAN) used evidence-based interventions (EBIs) for children with asthma to design community-based programs in a wide variety of settings—with varying resource constraints and priorities—that were often determined by the program context. Although challenges were faced, lessons learned strongly suggest that adapting and implementing EBIs is feasible in a variety of settings using a multisite approach. Lessons learned during the MCAN initiative presented unique opportunities to refine best practices that proved to be important to translation of EBIs in community-based settings. The adopted best practices were based on experiential learning during different phases of the project cycle, including monitoring and evaluation, translational research, and implementing policies in local program environments. Throughout this discussion it is important to note the importance of program context in determining the effectiveness of the interventions, opportunities to scale them, their affordability, and the ability to sustain them. Lessons learned from this effort will be important not only to advance science-based approaches to manage childhood asthma but also to assist in closing the gap between intervention development (discovery) and program dissemination and implementation (delivery).
Serological Response of Shiga Toxin-Producing Escherichia coli Type III Secreted Proteins in Sera from Vaccinated Rabbits, Naturally Infected Cattle, and Humans

ABSTRACT Escherichia coli O157:H7 is an important zoonotic pathogen, causing hemolytic uremic syndrome (HUS). The colonization of cattle and human hosts is mediated through the action of effectors secreted via a type III secretion system (T3SS). The structural genes for the T3SS and many of the secreted effectors are located on a pathogenicity island called the locus of enterocyte effacement (LEE). We cloned and expressed the genes coding for 66 effectors and purified each to measure the cross-reactivity of type III secreted proteins from Shiga toxin-producing Escherichia coli (STEC) serotypes. These included 37 LEE-encoded proteins and 29 non-LEE effectors. The serological response against each protein was measured by Western blot analysis and enzyme-linked immunosorbent assay (ELISA) using sera from rabbits immunized with type III secreted proteins (T3SPs) from four STEC serotypes, experimentally infected cattle, and human sera from six HUS patients. Twenty proteins were recognized by at least one of the STEC T3SP-vaccinated rabbits by Western blotting. Several structural proteins (EspA, EspB, and EspD) and a number of effectors (Tir, NleA, and TccP) were recognized by O26-, O103-, O111-, and O157-specific sera. Sera from experimentally infected cattle and HUS patients were tested using an ELISA against each of the proteins. Tir, EspB, EspD, EspA, and NleA were recognized by the majority of the samples tested. A number of other proteins also were recognized by individual serum samples. Overall, proteins such as Tir, EspB, EspD, NleA, and EspA were highly immunogenic in vaccinated and naturally infected subjects and could be candidates for a cross-protective STEC vaccine.
Preparing for Bike-Sharing

Objectives. To obtain in-depth community input using qualitative and quantitative methods to guide development and marketing of a bike-share program in Chattanooga, Tennessee. Method. Focus groups and surveys assessed bicycling attitudes, beliefs, barriers, and behaviors of residents, workers, and university students. The authors completed nine focus groups (N = 56): five sessions with downtown workers, three with downtown residents, and one with university students. Results. Health, recreation and transportation benefits of bicycling were commonly identified. Concerns regarding bicycling in traffic are apparent because of lack of facilities and a need for public education on safe motorist and bicyclist behavior. Practical limitations can inhibit bicycling during the day, including shower access and personal hygiene concerns. Conclusions. Public desire for environmental, educational, and enforcement tactics to support safe bicycling was noted. Marketing tactics for bike-share usage should emphasize health, recreational, and transportation benefits. Worksites can reduce barriers related to bicycling and encourage bike-share use. Future studies should assess bike-share impact on perceptions and behavior, as well as the resulting policy and environmental changes.
Making Health Promotion Evidenced-Informed

This large urban health unit identified a need for explicit, strategic, long-term organizational priority toward practical application of evidence in health promotion practice. Becoming a Best Practice Spotlight Organization (BPSO®) candidate provided an opportunity to systematically implement this commitment. The primary goals were to support incorporation of evidence-informed practice throughout the organization, increase interprofessional collaboration, and provide opportunities for knowledge exchange for staff. A mixed-methods evaluation consisting of three phases, including an analysis of previous evaluations, a survey of Champions, and an online focus group with the Steering Committee, demonstrated very positive outcomes. Staff reported increased incorporation of evidence in practice and program delivery. Collaboration and consultation amongst interdisciplinary staff across program areas also increased and staff responded very positively to increased opportunities for knowledge exchange. BPSO® candidacy opportunities should be used by health organizations to increase evidence-informed practice and inspire excellence in health promotion practice.
Strain-Specific Neutralizing Antibody Responses against Human Cytomegalovirus Envelope Glycoprotein N

ABSTRACT The human cytomegalovirus (HCMV) gM-gN complex is a major target of virus-neutralizing activity, and gN subtypes induce strain-specific antibodies. However, the biological significance of HCMV gN polymorphisms is not known. Neutralizing antibody responses against HCMV gN recombinant viruses were investigated at study entry in 80 healthy HCMV-seropositive women who were monitored for the appearance of new antibody specificities against linear strain-specific epitopes on glycoproteins gH and gB as evidence of HCMV reinfection. Neutralizing activity against all four gN recombinant viruses was seen in 74% of subjects, and 61% of subjects had strain-specific responses. Significantly fewer women (9/39 subjects [23%]) with serological evidence of reinfection had strain-specific neutralizing responses than the women without reinfection (21/41 subjects [51%]). Women with antibodies against at least one of the four linear gB and gH antigens at study entry had higher neutralizing titers against gN-1 (P = 0.006) and gN-2 (P = 0.007). Neutralizing titers of ≥400 against gN-3 (P = 0.043) and gN-4 (P = 0.049) at study entry were associated with longer times to serological evidence of reinfection. The findings demonstrate that HCMV gN elicits strain-specific neutralizing antibody responses and that broader anti-gN neutralizing activity may provide some protection from reinfection with a different virus strain.
A single-carrier/OFDM comparison for broadband wireless communication

OFDM is a popular modulation scheme for broadband wireless communication. It elegantly handles multipath and has low complexity. Recently, single-carrier with frequency-domain processing (SC-FD) is gaining attention as a possible competitor for OFDM. It is based on the same principles and, therefore, it has similar multipath handling capabilities and low complexity. Moreover, SC-FD promises several other attractive advantages, such as power amplifier efficiency and inherent frequency diversity exploitation. Current performance comparisons do not consider these effects combined. We make the comparison in a realistic scenario considering both the power amplifier efficiency and frequency diversity. Our results show that, in a multipath environment, SC-FD can outperform OFDM by 4 dB, largely due to the better frequency diversity exploitation. Therefore, SC-FD is a good alternative to OFDM for battery-powered terminals, and is therefore especially suited for uplink communication.