Miracle-dz/UniFAR-FTUs huggingface dataset:
Utilización de Aceite Esencial de Lemongrass (Cymbopogon citratus Stapf) Como Repelente de Diuraphis noxia Kurdj. (Hemiptera: Aphididae) en Trigo

Una herramienta alternativa para el manejo integrado de plagas, es la utilizacion de productos naturales, como aceites esenciales y extractos vegetales. El objetivo del trabajo fue evaluar la actividad repelente del aceite esencial de lemongrass (Cymbopogon citratus Stapf) sobre el pulgon ruso (Diuraphis noxia Kurdj.) en trigo (Triticum aestivum L.). El aceite esencial se formulo en solucion acuosa empleando como emulsionantes propilenglicol al 5% y lecitina de soja al 0,5%. Las concentraciones evaluadas fueron 0,5; 1; 2; 3 y 4% y testigos en blanco, con cinco repeticiones por tratamiento. Se utilizaron dos tecnicas de aplicacion: impregnacion de papeles y pulverizacion directa, cuando las plantas de trigo se encontraban al estado de dos hojas completamente desarrolladas. Luego de la aplicacion, se colocaron 10 pulgones adultos por maceta y se realizo el recuento a las 6 h. Los resultados obtenidos se transformaron en porcentaje de repelencia y se analizaron por ANDEVA y test de Tukey (P = 0,05). Para las tecnicas de aplicacion, se obtuvo un porcentaje de repelencia de 66,07 y 74,33% para pulverizacion e impregnacion de papeles, respectivamente. Al evaluar las formulaciones con los dos emulsionantes, lecitina de soja y propilenglicol, el porcentaje de repelencia fue de 64,78 y 75,63%, respectivamente. Para las concentraciones de lemongrass, el porcentaje de repelencia oscilo entre 65 y 89%. Se concluye que los volatiles de C. citratus demostraron poseer actividad repelente sobre D. noxia.
Clinical management of benign joint hypermobility syndrome: a case series.

Background Benign Joint Hypermobility Syndrome (BJHS) is a relatively prevalent condition of the spectrum of heritable connective tissue disorders, with musculoskeletal, visceral and psychological manifestations. The conservative management of the musculoskeletal symptomatology must be modified for optimal effectiveness and minimal sequelae. Purpose To provide an overview of the presentation, assessment, chiropractic management, and outcomes of patients with BJHS. Study Design Case series. Discussion Recognizing joint hypermobility as a significant contributing factor in patients presenting with musculoskeletal complaints is often challenging. The lack of awareness of BJHS may delay the diagnosis as well as effective management. Manual therapy should be used judiciously; active exercise is an essential element of care. We provide an overview of the presentations, assessment, chiropractic management, and outcomes of three patients with BJHS. Future clinical trials are necessary to determine effective clinical management strategies for patients with BJHS.
Evaluation of type II toxin-antitoxin systems, antibiotic resistance profiles, and biofilm quorum sensing genes in Acinetobacter baumannii isolates in Iraq.

BACKGROUND Bacterial Toxin-Antitoxin (TAs) systems are extensive two-component elements in the bacterial genome, which involved in many key biological functions including growth arrest, survival, biofilm formation, plasmid maintenance, defense against phages, persistence and virulence. AIM This study aimed to assess the molecular determinants involved in TAs, biofilm quorum sensing, and antibiotic resistance profiles in Acinetobacter baumannii isolated from Baghdad`s hospitals in Iraq. METHODS A total of 127 A. baumannii isolates were collected from 2160 different clinical samples. The antimicrobial susceptibility test was performed using disk diffusion test. All isolates were characterized for molecular determinants involved in TAs and biofilm formation using the well-known PCR-based sequencing assay. RESULTS A high multi-drug resistant (MDR) (96.06%; 122/127 ) and imipenem resistance (84.25%; 107/127 ) rates were observed from A.baumannii isolates. Results showed the presence of rhlIR gene in three isolates (2.36%), and lasIR gene appeared in two isolates (1.57%) isolates, whilst, mazEF, ccdAB, and relBE genes have not detected among isolates. CONCLUSION A high MDR and imipenem resistance rates within a low prevalence of rhlIR, and lasIR genes could be found in clinical A. baumannii isolates from some of Iraqi hospitals.
Estimation of Dietary Sodium Bicarbonate Dose Limit in Broiler under High Ambient Temperatures

A six-week trial was conducted to estimate the dose limit of sodium bicarbonate (SB) in the diet of broiler chickens exposed to high ambient temperature. The estimation of SB dose limit was based on the growth performance, blood biochemical parameters and histological observation of the renal tissues of chickens. A total of 450 dayold male broilers were randomly distributed to five groups with SB supplemented at levels of 0 (control), 0.5%, 1.0%, 2.0% and 4.0%. The minimum and maximum average ambient temperatures were 26.4°C and 32.5°C, respectively, with relative humidity ranging from 57.2% to 83.8%. Results showed that (1) A linear effect of increasing dietary SB level was observed on body weight gain (BWG) (P<0.05), feed intake (FI) (P<0.05) and feed to gain ratio (F:G) (P <0.05) from day 1 to 21 and day 1 to 42. Supplementation of 4.0% SB decreased BWG (P<0.001) and FI (P< 0.001), but increased F: G (P<0.001) from day 1 to 21, and decreased FI (P<0.001) from day 1 to 42. (2) Compared with the control treatment, an SB of 4.0% gave higher incidences of diarrhea (P<0.001), mortality (P<0.001) and ascites related mortality (P<0.001). (3) Rectal temperatures were linearly decreased (P<0.05) with rising SB levels. (4) Blood biochemical analysis indicated that SB higher than 1.0% decreased blood hemoglobin (HGB) (P<0.001), and increased hematocrit (HCT) (P<0.001) and serum malondialdehyde (MDA) concentration (P<0.001) on day 21. (5) Microscopic examination revealed that significant renal haemorrhage appeared when SB exceeded 1.0% and tubular dilation was observed in 4.0% SB treatment. In summary, dietary supplementation of 4.0% SB resulted in poor growth performance and higher mortality. In addition, SB higher than 1.0% affected blood biochemical parameters and caused renal lesions. The current results suggest that the dose limit of dietary SB is less than 1.0% for broilers under high ambient temperatures.
The chromatin-modifying protein HUB2 is involved in the regulation of lignin composition in xylem vessels

Abstract PIRIN2 (PRN2) was earlier reported to suppress syringyl (S)-type lignin accumulation of xylem vessels of Arabidopsis thaliana. In the present study, we report yeast two-hybrid results supporting the interaction of PRN2 with HISTONE MONOUBIQUITINATION2 (HUB2) in Arabidopsis. HUB2 has been previously implicated in several plant developmental processes, but not in lignification. Interaction between PRN2 and HUB2 was verified by β-galactosidase enzymatic and co-immunoprecipitation assays. HUB2 promoted the deposition of S-type lignin in the secondary cell walls of both stem and hypocotyl tissues, as analysed by pyrolysis-GC/MS. Chemical fingerprinting of individual xylem vessel cell walls by Raman and Fourier transform infrared microspectroscopy supported the function of HUB2 in lignin deposition. These results, together with a genetic analysis of the hub2 prn2 double mutant, support the antagonistic function of PRN2 and HUB2 in deposition of S-type lignin. Transcriptome analyses indicated the opposite regulation of the S-type lignin biosynthetic gene FERULATE-5-HYDROXYLASE1 by PRN2 and HUB2 as the underlying mechanism. PRN2 and HUB2 promoter activities co-localized in cells neighbouring the xylem vessel elements, suggesting that the S-type lignin-promoting function of HUB2 is antagonized by PRN2 for the benefit of the guaiacyl (G)-type lignin enrichment of the neighbouring xylem vessel elements.
Reducing dropout in acceptance and commitment therapy, mindfulness-based cognitive therapy, and problem-solving therapy for chronic pain and cancer patients using motivational interviewing.

OBJECTIVE Acceptance and commitment therapy, mindfulness-based cognitive therapy, and problem-solving therapy are types of cognitive-behavioural therapy (CBT) group that improve physical and mental health in chronic pain or cancer. However, dropout is high due to group demands alongside physical impairments. Motivational interviewing (MI) is a well-evidenced means of enhancing treatment adherence. Few studies have investigated MI as an adjunct to CBT in cancer or chronic pain, and none have established the minimum MI duration required for adherence improvement. This study evaluated minimal-duration MI to improve adherence in three CBT group types for cancer and chronic pain. METHODS In a cohort study of 99 cancer and chronic pain patients, 47 were given a 10- to 15-min structured MI telephone intervention (MI-call) after the first session. The remaining 52 received a CBT group without MI (no-MI). RESULTS Odds of completing group CBTs were five times greater for patients in the MI-call cohort versus no-MI. Effects remained when controlling for age, gender, diagnosis, group type, and baseline quality of life. The MI-call cohort attended one extra session per patient compared to no-MI, controlling for age, gender, and diagnosis. CONCLUSIONS A brief MI telephone intervention may improve adherence to group CBTs in cancer and chronic pain. PRACTITIONER POINTS A brief motivational interviewing (MI) telephone intervention may reduce dropout from group cognitive-behavioural therapies (CBTs) for cancer and chronic pain patients when administered after the first group session in routine care. Recipients of this intervention were five times more likely to complete a group CBT programme than those who did not receive it. Therefore, a minimal-dose MI intervention can have clinically important effects on dropout in group CBTs for patients with long-term conditions. It is unclear whether this intervention would also result in greater outcome improvements.
CORRELAÇÃO ENTRE PARÂMETROS BIOMÉTRICOS E PRODUTIVOS EM COLÔNIAS DE Melipona quadrifasciata anthidioides LEPELETIER (HYMENOPTERA: APIDAE)

In meliponini colonies, biometric characteristics may be associated with production traits, thus the study of correlations is extremely useful as a tool for colony selection process. The aim of this study was to estimate the correlations between biometric and productive parameters of Melipona quadrifasciata anthidioides. We analyzed 128 colonies, from 60 parental and two generations, F1 and F2. The following parameters were evaluated: queen and colony weight; number, length and width of brood disks; number, width, depth and volume of honey pots; number, width and depth of pollen pots; glossa size, and estimate of the population and honey production. The queen weight was correlated with the number of brood disks (0.23) and population (0.23), as well as the characteristic number of pollen pots is related to the length and width of brood disks and population (0.88, 0.54 and 0.52, respectively). The characteristic honey production is related to the number (0.93), width (0.50) and volume (0.47) of honey pots. The results showed that honey production is directly correlated with number, volume, width and depth of honey pots. On the other side, the population size was correlated with the number of brood disks and pollen pots.
The Effect of Experimental Clutch Harvest on Numbers of Magpie Goose Nests and Juvenile Recruitment

The effect of harvest of magpie goose clutches on nest numbers and juvenile recruitment was investigated experimentally within a single breeding colony, by comparing the densities of nests and offspring (eggs and goslings) produced in harvested and unharvested blocks in each of two successive years. When adjusted for initial differences in nest density, by covariance analysis, there were significantly higher densities of nests in harvested blocks than in unharvested blocks, possibly due to re-laying in new nests by those geese whose clutches were harvested. There were no differences in densities of offspring produced between harvested and unharvested blocks although there were differences in densities of offspring produced between years.
Analysis of factors related to fertility after endometriosis combined with infertility laparoscopic surgery

Abstract To investigate the influence factors of laparoscopic postoperative pregnancy of patients with endometriosis and infertility, further validate the application of EFI scoring system in endometriosis, and to improve the pregnancy rate. A total of 258 patients with endometriosis and infertility who underwent laparoscopic surgery and follow-up treatment at Wuxi Maternal and Child Health Hospital from January 2015 to December 2016 were selected and divided into pregnant and non-pregnant groups according to whether they were pregnant. All patients were divided into 4 groups according to EFI score: group with EFI score ≥9, 7–8, 4–6, and <4, and divided into I, II, III, and IV groups according to AFS stages. The uterus-laparoscopic surgery was performed. The patients were followed up for 3 years. The factors affecting the pregnancy rate were analyzed. The pregnancy rate and pregnancy types were calculated at different time points. Multivariate analysis showed that age <35 years, infertility time <5 years, secondary infertility, EFI score, postoperative ART application were protection factors of postoperative pregnancy. The 3-year cumulative postoperative pregnancy rate was 75.6%. The cumulative pregnancy rate was 92.2% in group with EFI score ≥9, 85.9% in group with EFI score 7–8, 62.5% in group with EFI score 4–6 and 5.9% in group with EFI score <4, there was significant difference between the 4 groups (P < .05). The proportion of pregnancies in 6 months and 12 months was higher in patients with EFI score ≥7, 61.0% in patients with EFI score ≥9 and 41.1% in patients with EFI score ≥7. The highest natural pregnancy rate was 83.1% in group with EFI score ≥9, and there was significant difference between the 4 groups (P < .05). Age <35 years, infertility time <5 years, secondary infertility, EFI score and ART application were the protective factors of postoperative pregnancy. EFI score had positive significance in predicting and guiding the postoperative pregnancy of patients with endometriosis and infertility. According to EFI score, the pregnancy rate of patients with endometriosis and infertility can be significantly improved by strict management and active pregnancy program.
Interactions with PDZ proteins diversify voltage‐gated calcium channel signaling

Voltage‐gated Ca2+ (CaV) channels are crucial for neuronal excitability and synaptic transmission upon depolarization. Their properties in vivo are modulated by their interaction with a variety of scaffolding proteins. Such interactions can influence the function and localization of CaV channels, as well as their coupling to intracellular second messengers and regulatory pathways, thus amplifying their signaling potential. Among these scaffolding proteins, a subset of PDZ (postsynaptic density‐95, Drosophila discs‐large, and zona occludens)‐domain containing proteins play diverse roles in modulating CaV channel properties. At the presynaptic terminal, PDZ proteins enrich CaV channels in the active zone, enabling neurotransmitter release by maintaining a tight and vital link between channels and vesicles. In the postsynaptic density, these interactions are essential in regulating dendritic spine morphology and postsynaptic signaling cascades. In this review, we highlight the studies that demonstrate dynamic regulations of neuronal CaV channels by PDZ proteins. We discuss the role of PDZ proteins in controlling channel activity, regulating channel cell surface density, and influencing channel‐mediated downstream signaling events. We highlight the importance of PDZ protein regulations of CaV channels and evaluate the link between this regulatory effect and human disease.
Haloperidol’s Effect on the Expressions of TGFB, NT-3, and BDNF genes in Cultured Rat Microglia

Introduction: Microglia, small glial cells, i.e. mesodermal in origin and found in the brain and spinal cord, play a key role in the maintenance of neurons and immune defense. Haloperidol, an antipsychotic drug, is used to treat numerous neurological and neurodegenerative disorders. Its mechanism is not understood; however, haloperidol may result in Wnt signaling pathway activation. This study aimed to activate the Wnt signaling pathway using haloperidol and determining the effect of GSK3 inhibition on the expression of TGFB, NT-3, and BDNF genes in cultured rat microglia. Methods: Microglia isolation was conducted, and the immunohistochemistry technique was performed to confirm microglia purity. The RNA extraction was followed by cDNA synthesis. Real-time RT-PCR was used to evaluate any significant changes in the expression level of these genes. Results: The three gene expressions in microglia were proportional to the different concentrations of the drug. More concentration of drugs resulted in higher levels of expression of these genes. Besides, the haloperidol did not affect the expression of the beta-actin gene as the reference gene. Conclusion: The obtained results supported the beneficial use of haloperidol in targeted microglia therapy. This study can be a breakthrough in neurology research.
Amelioration of Heavy-Metal Toxicity in Cauliflower by Application of Salicylic Acid

The important role of salicylic acid in response to different stresses is to modify and decrease the negative effects of stress. The objective of this study was to evaluate the amelioration of heavy-metal (HM) stress in cauliflower cv. Shubra by application of different concentrations of salicylic acid. In heavy-metal (cobalt, nickel, cadmium, chromium, and lead) stress, apart from appearance of visual symptoms, HM toxicity reduced dry weight and specific activity of catalase and increased the concentration of lipid peroxidation, proline, nonprotein thiol, electrolyte leakage percentage, and specific activity of peroxidase and superoxidase dismutase. Application of salicylic acid reverts back all parameters disturbed by HM stress. As opposed to other HMs, salicylic acid enhanced chromium toxicity effects in plants, showing synergism and the plants died. Application of salicylic acid (100 mM) ameliorated toxic effects of HMs to some extent, showing antagonism in cauliflower.
Risk Profiles in Heart Failure

Development of new-onset HF significantly accentuates risk for hospitalization and mortality compared with age-matched controls without HF. This baseline risk is associated with potential for short-term deterioration in the absence of optimal and aggressive use of guidelinedirected therapy, treatment of comorbidities, and close follow-up. Subsequent to implementation of optimal therapy, many patients experience variable durations of clinical stability with persistent residual risk for adverse outcomes, including sudden death. While residual risk on optimal therapy is lower than baseline risk, it is nevertheless comparable to or worse than other major cardiovascular conditions and substantially higher than individuals without HF. Hence, there is a growing realization that the term chronic stable HF is misleading if cardiac structure and function remains deranged, even if symptoms have stabilized or no longer evident.1 Many stabilized patients with residual risk have episodes of worsening HF (WHF) traditionally defined by need for hospitalization.2 Recognition of this worsening risk profile has led to prevention of HF hospitalization as a common focal point for clinical trials and quality improvement initiatives, all with the goal of reducing high rates of post-discharge mortality and readmission. Finally, a proportion of patients with worsening risk become refractory to medical therapy and enter a phase of advanced risk, where in the absence of mechanical circulatory support or transplantation they succumb to the disease and die.
Reproductive biology and sexual dimorphism of a high-altitude population of the viviparous lizard Phymaturus punae from the Andes in Argentina

Phymaturus punae is a viviparous lizard endemic to the Provincial Reserve San Guillermo, San Juan (Argentina). We present the first life history data of P. punae , belonging to an interesting genus adapted to cold climates. Females reach sexual maturity at 80.6 mm, and males at 84.4 mm snout-vent length. There is sexual dimorphism represented by larger and more robust males, but females show a wider body and a proportionally greater interlimb length than males. In males, spermatozoa become abundant in both testes and epididymi in mid-summer. Vitellogenic and pregnant females occurred simultaneously in late spring and mid-summer indicating a biennial reproductive cycle.
Detection of Clostridioides difficile by Real-time PCR in Young Children Does Not Predict Disease.

OBJECTIVES Diagnosing Clostridioides difficile infections in young children with high asymptomatic colonization is challenging. We compared the frequency of C difficile detection by polymerase chain reaction (PCR) in healthy control (HC) children with those with acute gastroenteritis (AGE) and evaluated fecal-lactoferrin and organism load as possible indicators of true C difficile infection disease. METHODS Stool was collected from children <2 years old with AGE and from HCs. C difficile was detected by real-time PCR, and lactoferrin was measured by enzyme-linked immunosorbent assay. Clinical data were obtained via interviews and chart review. Mann-Whitney U test and χ2 tests were used for group comparisons. RESULTS Of 524 stools collected from 524 children (250 with AGE, 274 HCs), C difficile was detected less in children with AGE (14%, 36 of 250) than in HCs (28%, 76 of 274) stools (P < .0001). Among infants <1 year old (n = 297), C difficile was detected in 18% of children with AGE versus 32% of HCs (P < .005), and among children 1 to 2 years old (n = 227), C difficile was detected in 10% of children with AGE versus 21% of HCs (P < .02). There was no significant difference in C difficile PCR cycle threshold values between children with AGE and HCs or lactoferrin levels in C difficile PCR-positive versus -negative stools. CONCLUSIONS HC children <2 years of age had higher rates of C difficile detection by PCR than children with AGE; C difficile detection by real-time PCR alone is not a reliable means to diagnose C difficile disease in children <2 years old.
Influence of substrate on ultrafast water transport property of multilayer graphene coatings

The ultrafast water transport in graphene nanoplatelets (GNPs) coating is attributed to the low friction passages formed by pristine graphene and the hydrophilic functional groups which provide a strong interaction force to the water molecules. Here, we examine the influence of the supporting substrate on the ultrafast water transport property of multilayer graphene coatings experimentally and by computational modelling. Thermally cured GNPs manifesting ultrafast water permeation are coated on different substrate materials, namely aluminium, copper, iron and glass. The physical and chemical structures of the GNPs coatings which are affected by the substrate materials are characterized using various spectroscopy techniques. Experimentally, the water permeation and absorption tests evidence the significant influence of the substrate on the rapid water permeation property of GNPs-coating. The water transport rates of the GNPs coatings correspond to the wettability and the free surface energy of their substrates where the most hydrophilic substrate induces the highest water transport rate. In addition, we conduct molecular dynamics (MD) simulations to investigate the transport rate of water molecules through multilayer GNPs adjacent to different substrate materials. The MD simulations results agree well with the experimental results inferring the strong influence of the substrate materials on the fast water transport of GNPs. Therefore, selection of substrate has to be taken into consideration when the GNPs-coating is placed into applications.