Miracle-dz/UniFAR-FTUs huggingface dataset:
Application of the mixture design for optimum antimicrobial activity: Combined treatment of
 Syzygium aromaticum
 ,
 Cinnamomum zeylanicum
 ,
 Myrtus communis
 , and
 Lavandula stoechas
 essential oils against
 Escherichia coli

This study investigates the optimal combination of clove, cinnamon, lavender, and myrtle essential oils (EOs) against Escherichia coli. After the GC/MS analyses, the antimicrobial activities of the four EOs were screened. Afterward, a statistical mixture design established the right EOs’ combination capable of protecting milk from E. coli. Results illustrated that the EOs were chemically different and efficient against 14 bacteria. Hence, the simplex‐centroid mixture design was used to build polynomial models describing the relationship between the anti‐E. coli effect and the proportion of each EO. Result depicted that the lowest bacterial survivability was equal to 1.5 × 10⁶·CFU·ml⁻¹ for the combination of cinnamon and lavender; and the combination of cinnamon, lavender, and clove at a concentration of 2 mg/ml. The application of the statistical design concluded that the optimal combination corresponds to 2.4% S. aromaticum, 38.2% L. stoechas, and 59.4% C. zeylanicum. The combination validation exhibited its efficiency as it restricted the E. coli viability to 1 × 10⁶·CFU·ml⁻¹. PRACTICAL APPLICATIONS: EOs are the new eco‐friendly preservative for food. Their combination (using the right sources and proportions) allows better protection against germs. In this study, the obtained formula may be of interest in the milk and dairy industry as it contributes to protecting milk (and dairy products) against E. coli contamination.
Effect of Green tea on Heart Rate of Male and Female

Camellia sinensis (Green tea) as a hot decoction is widely used throughout the Asia. Therefore to find out their effects on all the body functions are the need of hour, to recommend its safe use. In the current study the effect of Camellia sinensis on human (male and females) heart rate has been studied, by giving one cup of hot decoction of green tea to each subject of fifty four male and fifty four females. The heart rate per minute before and after the decoction was evaluated as; a great increase in the heart rate had been noted in case of males. Also a great decrease in the heart rate of individuals has been identified in case of females. From the current study it can be concluded that green tea have an effect of increasing heart rate in males and decreasing in the females, so the heart patients have to take care while using green tea.
Susceptibilidad antimicrobiana in vitro de 1200 microorganismos Gram negativos causales de infecciones de vías urinarias

Objective. In vitro evaluation of 10 antimicrobial agents: amikacin, cefalexin, ceftibuten, cefuroxime, ciprofloxacin, gemifloxacin, netilmicin, moxifloxacin, levofloxacin, trimetoprim-sulfametoxazol, against Gram negative bacteria in outpatients with non-complicated urinary tract infections. Material and methods. Prospective study of antimicrobial sensitivity of 1,200 Gram negative bacteria isolated from the same number of patients of both sexes and all ages, with non complicated urinary tract infections, using the plate microdilution technique to determine MIC 50 and 90, and percents of susceptibility. Results. Most of isolates correspond to Escherichia coli: 638 (53.2%), followed by Klebsiella spp. : 166 (13.9%), Proteus spp. : 110 (9.1%), Morganella morganii: 84 (7.0%), Serratia spp. : 84 (7.0%), Enterobacter spp. : 66 (5.5%), and Citrobacter spp. : 52 (4.38%). The most active antimicrobials in terms of susceptibility and CIM 50 and 90 were ceftibuten and netilmicin. The other studied antibiotics show a very poor activity that varied according the isolated organism. Conclusions. In view of the results of this in vitro study, ceftibuten and netilmicin were the most active antimicrobials against the Gram negative etiological agents of the non-complicated urinary tract infections .
Atrichia with Papules - A Rare Condition

Observations: A 6 years old girl presented with total alopecia with papular lesions involving scalp and eyebrows. She was born with sparse hair and started losing hairs shortly after birth. Hairs did not grow thereafter. Papular lesions appeared later, some 2 years after alopecia. Based on classical history, diagnosis of atrichia with papules (AP) was made. Histopathological findings supported the diagnosis.
Comparison of biodegradation of lubricant wastes by Scenedesmus vacuolatus vs a microalgal consortium

Abstract Locally, the petroleum, aluminum and steel milling industries have expressed considerable interest in the utilization of bioremediation as a means for reducing their lubricant waste. Since hydrocarbons accumulate within microalgae, the aim of this study was to determine the efficiency of Scenedesmus vacuolatus vs a microalgal consortium (Chlamydomonas pitschmannii, Trebouxia australis and Pectinodesmus pectinatus) in the biodegradation lubricant waste products; from which these indigenous microalgae were originally isolated. To determine the biodegradation capability of these microalgae, dehydrogenase activity tests were carried out and indicated by % TPF values. Samples that tested positive for dehydrogenase activity, were analyzed using gas chromatography/mass spectrometry, to track the biotransformation of hydrocarbons intracellularly and extracellularly. Results were analyzed statistically with SPSS, p < 0.05. Dehydrogenase activity for both the coolant rolling oil (0.058 ± 0.001 mg/mL), and the spent rolling emulsion (0.047 ± 0.002) was higher for the microbial consortium compared to S. vacuolatus, 0.046 ± 0.001 mg/mL. Weekly, gas chromatography/mass spectrometry biotransformations, indicated no hydrocarbons present after five weeks, confirming 100% biodegradation. Irrespective of the use of either a single isolate or a microalgal consortium; complete biodegradation after just five weeks, makes the use of microalgae for bioremediation a viable strategy.
TEN COMMONLY AVAILABLE MEDICINAL PLANTS IN MALAYSIA USED FOR THE TREATMENT OF DIABETES – A REVIEW

Background: Diabetes mellitus is a common and serious metabolic disorder throughout the world. Search for newer drugs continues because the existing synthetic drugs have several limitations and side effects. Traditional plants have been used throughout the world for the treatment of diabetes. Objective: The present study is an attempt to list of the commonly available medicinal plants with antidiabetic and related beneficial effects originating from different parts of Malaysia. Method: This study was carried out for a general knowledge in which to give information on common medicinal plants in Malaysia used for the treatment of diabetes. Results: There are 10 such very commonly available medicinal plants were found and described in this review which clearly shows the importance of herbal plants in the treatment of diabetes. Conclusion: Only a few plants have been subjected to detailed scientific investigation. However, further pharmacological and toxicological studies at the molecular and clinical levels are still warranted to confirm the potential of these plants. Keywords: Medicinal plants, Malaysia, Diabetes
Factores de riesgo cardiovascular asociados a obesidad abdominal en adultos aparentemente sanos

Objetivo: determinar la relacion entre la obesidad abdominal y los principales factores de riesgo cardiovascular en una poblacion aparentemente sana. Material y metodos: estudio transversal comparativo al que se incluyeron 186 individuos aparentemente sanos, hombres y mujeres no embarazadas mayores de 18 anos, habitantes de la ciudad de Mexico. Se llevo a cabo historia medica detallada y exploracion fisica completa para colectar informacion sobre las variables antropometricas y de laboratorio. La obesidad abdominal se definio por un perimetro de cintura ≥ 80 cm en la mujer y ≥ 90 cm en el hombre. Resultados: se integraron 125 mujeres (67.2 %) y 61 hombres (32.8 %); 151 sujetos (81.2 %) presentaron resistencia a la insulina y 130 (69.9 %), obesidad abdominal; de estos ultimos, 96 (46.2 %) presentaron sindrome metabolico. Se encontro elevada prevalencia de hipertrigliceridemia (31 %) y niveles bajos de HDL-colesterol (58 %) en la poblacion sin obesidad abdominal. Conclusiones: aun cuando identifica a una elevada proporcion de sujetos con factores de riesgo cardiovascular, el punto de corte utilizado para definir obesidad abdominal no reconocio una importante proporcion de individuos con alteraciones en el perfil de lipidos
Privacy, Confidentiality and Consent of Electronic Health Record Systems

Confidentiality plays an important role in the success of electronic health record systems. Health record systems either contain or make direct reference to sensitive health data for individual patients. Electronic health data increases the accessibility of data from different healthcare providers. This causes concern over the patient's privacy. Privacy of health information rates as one of the most significant causes of concern from consumers (Puplick 2003) and healthcare providers. Informed patient consent and access permissions to the electronic records are barriers in achieving the full adoption of electronic health record in most healthcare institutions (Protti 2002). University of Wollongong has developed a prototype for the electronic health record systems. Implementing patients' consent in electronic health records increases in consumer trust and assists in moving towards full adoption of electronic health record systems. This paper aims to demonstrate that viable and usable e-consent mechanism can be developed using working prototype as an example.
Extracts from Rhizopus oryzae KSD-815 of Korean Traditional Nuruk Confer the Potential to Inhibit Hypertension, Platelet Aggregation, and Cancer Metastasis in vitro

Rhizopus oryzae KSD-815 was isolated from nuruk that has been used to make Korean traditional wines. This study was performed to investigate the effect of cultures of R. oryzae KSD-815 on cardiovascular disorders and cancer metastasis. Firstly, these cultures were sequentially fractionationed with n-hexane (TAHe), ethylacetate (TAE), n-butanol (TAB), and H₂O (TAW). The TAE inhibited the activity of angiotensin-converting enzyme (ACE) and TAB suppressed platelet aggregation in vitro. TAE and TAB inhibited cell motility of human breast cancer cells. Furthermore, TAW interrupted the formation of neovasculature and tube-like structure, and down-regulated the expression of angiogenic factors, basic fibroblast growth factor (bFGF), tumor necrosis factor-α (TNF-α), and hypoxia-inducible factor-1α (HIF-1α) in breast cancer cells. These results indicated that cultures of R. oryzae KSD-815 display the inhibitory activities on hypertension, platelet aggregation, and metastasis, and suggest that these cultures might be further probed for the purposes as therapeutic agents or dietary supplements.
Penambahan Enzim Fitase Pada Ransum dengan Level Protein Berbeda Terhadap Efisiensi Protein Ayam Broiler

Penelitian ini bertujuan untuk mengkaji penggunaan enzim fitase dalam ransum pada taraf protein yang tepat untuk meningkatkan efisiensi penggunaan protein ayam broiler. Materi yang digunakan dalam penelitian adalah ayam broiler dengan bobot badan 108,14 ± 11,44 g (CV 10,58%) umur 1 minggu sebanyak 128 ekor (unsex) yang dipelihara dalam 16 unit kandang selama 6 minggu, masing-masing unit berisi 8 ekor ayam. Penambahan fitase dilakukan pada minggu kedua pemeliharaan. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan 4 perlakuan dan 4 ulangan yaitu T0 (Ransum protein 23%), T1 (Ransum protein 21% + fitase 1000 FTU/kg), T2 (Ransum protein 23% + enzim fitase 1000 FTU/kg), T3 (Ransum protein 23% + mineral 1%). Data dianalisis ragam menggunakan uji F pada taraf 5%, jika ada pengaruh perlakuan dilanjutkan dengan uji duncan untuk melihat perbedaan antar perlakuan. Parameter penelitian meliputi konsumsi protein, kecernaan protein ileal dan rasio efisienfi protein. Hasil penelitian perlakuan menunjukkan berpengaruh nyata (P<0,05) terhadap konsumsi protein, kecernaan protein ileal dan rasio efisiensi protein. Kesimpulan dari penelitian ini adalah ransum protein 21% yang ditambahkan fitase menunjukkan hasil efisiensi protein yang lebih baik dibandingkan ransum kontrol, ransum protein 23% yang ditambah fitase maupun ransum protein 23% yang ditambahkan mineral.
Growth, photophysiology and photosynthesis of two marine diatoms grown under predictable intra-diel light fluctuations

Natural diatom populations experience variability in irradiance because of several physical processes. Laboratory studies often neglect this and use a static light environment. Consequently, the way dynamic light environments impact growth and photophysiology of diatoms is poorly understood. To address this, several aspects of photophysiology were measured in the diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum when grown under square-wave (SQ), sinusoidal (SI), and low (LF) and high (HF) amplitude light fluctuations of a 1-hour period each with the same light dose. Acclimation to increasing light fluctuation amplitude was found to be functionally similar to high light acclimation. Chlorophyll-a specific light absorption coefficients, maximum photosystem II electron transport rates (ETR) and the capacity for non-photochemical quenching (NPQ) all increased from SI to HF cultures. In both species increasing light fluctuation amplitude reduced growth rate. This reduction was greater for T. pseudonana than P. tricornutum. In HF cultures growth rates were 50% and 62% of those in SQ cultures respectively for the two species. Similar daily ETR between SI and LF cultures of P. tricornutum suggested that differences in the photosynthetic efficiency of light utilisation were a poor explanation for the lower growth rates in LF cultures. Lower growth rate under fluctuating light was instead hypothesised to be caused by greater photodamage and energy investment in photoprotection. Higher NPQ in P. tricornutum reduced net photodamage compared with T. pseudonana. However, light harvesting and ETR in P. tricornutum appeared to be adapted to a lower light environment than T. pseudonana. Higher amplitude light fluctuations also decreased intradiel variability in photoacclimation. This response was greater in P. tricornutum and was responsible for this species ability to maintain a consistent daily ETR between SI and LF regimes. This is thought to give P. tricornutum a competitive advantage in more dynamic light environments.
A multicenter controlled, randomized, open trial of interferon alpha2b treatment of anti-human immunodeficiency virus-negative hemophilic patients with chronic hepatitis C. Hepatitis Study Group of the Association of Italian Hemophilia Centers.

There is limited information about the long-term efficacy of prolonged therapy (more than 6 months) with interferon alpha in hemophilic patients with chronic hepatitis C who are not coinfected with the human immunodeficiency virus (HIV-1). One hundred and seven hemophiliacs were randomly assigned to 3 million U of interferon alpha2b three times weekly for 12 months or no therapy. The patients were followed up for at least 12 months posttreatment. Response was assessed by both serial alanine aminotransferase (ALT) levels and hepatitis C virus (HCV)-RNA measured by reverse transcribed polymerase chain reaction (RT-PCR) method. Before treatment, serum levels of HCV-RNA were measured quantitatively by second-generation branched-DNA assay and the HCV genotype was determined by RT-PCR. Serum HGV-RNA, a marker of infection with the hepatitis G virus, was also measured by RT-PCR. Normalization of ALT was sustained and serum HCV-RNA was cleared in 6 of 45 treated patients, compared with none of the 50 untreated controls (13% v 0% P < .01). Low pretreatment viremia was the only feature that was associated with an increased likelihood of sustained response (P < .01). This study shows that multitransfused hemophiliacs with chronic hepatitis C not coinfected with HIV-1 respond at low rates to prolonged interferon therapy.
Antibacterial properties of carboxymethyl chitosan Schiff-base nanocomposites loaded with silver nanoparticles

Abstract Nanocomposites of anisaldehyde and salicylaldehyde carboxymethyl chitosan (CMC)-Schiff bases/silver nanoparticles have been prepared and characterized. Their antibacterial activities were evaluated against (Bacillus Subtilis, Staphylococcus aureus and Streptococcus faecalis) as Gram + ve bacteria and the Gram − ve bacteria (Escherichia coli, Neisseria gonorrhoeae and Pseudomonas aeruginosa). The prepared compounds were characterized using FTIR spectroscopy, scanning electron microscopy, transmission electron microscopy and thermogravimetric analysis. Determination of water uptake of both CMC-Schiff bases/silver nanoparticles was carried out. Minimum inhibitory concentrations (MIC) of the prepared nanocomposites were determined as well. Cytotoxic activity of the investigated nanocomposites were evaluated against liver carcinoma cell; HEPG2 and breast cell line; MCF7. Graphical Abstract
Dysregulation of microRNA expression in acquired endocrine-resistant breast cancer.

DYSREGULA nON OF MICRORNA EXPRESSION IN ACQUIRED ENDOCRINE-RESISTANT BREAST CANCER Tissa T. Manavalan September 20, 2012 MicroRNAs (miRNAs) regulate gene expression at the post-transcriptional level by repressing translation or stimulating mRNA degradation. In this study, I tested the hypothesis that miRNAs are differentially expressed in antiestrogensensitive MCF-7 versus -resistant L Y2 human breast cancer cells. Microarray analyses identified 97 miRNAs that are differentially expressed between two estrogen receptor alpha (ERa) -positive human breast cancer cell lines: endocrine-sensitive MCF-7 versus -resistant L Y2 cells under basal conditions. Opposite expression of miRs-lOa, -21, -22, -12Sb, -181, -200a, -200b, -200c, -221, and -222 was confirmed between MCF-7 and L Y2 cells. The ER antagonist ICI 182,780 (fulvestrant or Faslodex) generally blocked the effect of estradiol E2 and 4-hydroxytamoxifen (4OHT) regulated miRs, i.e . , miR-lOa, miR-21, miR-22, miR-200a, miR-221, and miR222, indicating that these responses in MCF-7 cells are ER-mediated. Timedependent variation in basal (ethanol, the vehicle), E2, and 4-0HT regulation of the top 8 miRNAs was detected in MCF-7 cells. Bioinformatic analyses to impute the biological significance of the identified miRNAs by identifying their computationally predicted target genes in the human genome using TargetScan, Pic Tar, and the Sanger
Does Oxidizing Power of Roots Affect the Iron Toxicity Tolerance of Rice

Three varieties of rice with varying iron toxicity tolerance were used in an experiment conducted at the Regional Rice Research and Developing Centre, Bombuwela. The varieties were Bw 267-3 (tolerant), Bw 272-6b (susceptible) and Bw 363 (moderately tolerant). Rice seedlings were grown in a nursery maintaining aseptic conditions. To test the iron toxicity tolerance by rice varieties 21 day-old seedlings were transplanted in sand culture maintained at saturated condition and enriched with three iron treatments; 0, 75 and 300 ppm of Fe supplied as FeSO4.7H2O in four replicates. Plants were irrigated with standard IRRI solution with the respective iron treatment twice a week until 40 days after germination (DAG). At 40 DAG plants were uprooted and oven dry weights, shoot Fe, P and K contents were measured. Potential rhizosphere oxidizing power of three rice varieties was assessed following the method suggested by Trolldenier (1987). Analysis of variance was done to determine the significance of treatment effects and LSD was used for mean separation.
Microwave-assisted synthesis of coumarin-based 5,6-dihydro pyrimidin-2(1H)-one derivatives

Coumarin is an oxygen-containing heterocyclic compound of great medicinal important and high versatility in electronic and material science research. Incorporation of pyrimidine as a linker on coumarin is a way to provide improved application in solar cell research by extension of unsaturation for improved electronic transition. Convention synthesis approach is common in organic synthesis but have some disadvantages like affecting the eco-system due to discharge of toxic chemical during the process. On the other hand, microwave irradiation is eco-friendly and accelerates synthesis to afford great products following interesting reaction schemes and steps at reduced time. Hence, microwave assisted synthesis of novel coumarin-based 5,6-dihydropyrimidin-2(1H)-one derivatives was herein achieved via a three-step synthetic approach. The reaction was initiated with catalyst supported multicomponent reaction to produce 3-acetylcoumarin 7 which upon condensation with five aromatic aldehydes furnished chalcones 8a-e. Microwave assisted reaction of chalcones 8a-e with lone pair donor, urea led to the formation of the targeted coumarin-based 5,6-dihydropyrimidin-2(1H)-one 9a-e in good-to-excellent yields. The structures were established using spectroscopic data and notable physical properties and the results obtained were consistent with the expected structure of the products. The compounds will be good for further study to authenticate their applications in drug design and material science research.
Reusing Cow Manure for the Production of Activated Carbon Using Potassium Hydroxide (KOH) Activation Process and Its Liquid-Phase Adsorption Performance

In this work, cow manure (CM) was reused as a potential precursor in the production of activated carbon (AC) using a potassium hydroxide activation process at different temperatures (i.e., 500, 600 and 700 °C). The optimal activated carbon from cow manure (CM-AC) with high specific surface area (ca. 950 m2/g) was further investigated for its adsorption performance in the removal of a model compound (i.e., methylene blue) from aqueous solution with various initial concentrations and adsorbent dosages at 25 °C. It was found that the resulting AC could be an effective adsorbent for removal of cationic dye from aqueous solution in comparison with a commercial coal-based AC. Based on the observations of the energy dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy (FTIR), the CM-AC adsorbent has a stronger interaction with the cationic compound due to its more oxygen-containing complex on the surface. Furthermore, the adsorption kinetic parameters fitted using the pseudo-second order model with high correlations were in accordance with their pore properties.