Miracle-dz/UniFAR-FTUs huggingface dataset:
Validation and optimization of experimental colitis induction in rats using 2, 4, 6-trinitrobenzene sulfonic acid

Trinitrobenzene sulfonic acid (TNBS)-induced colitis is one of the most common methods for studying inflammatory bowel disease in animal models. Several factors may, however, affect its reproducibility, rate of animal mortality, and macroscopic and histopathological outcomes. Our aim was to validate the main contributing factors to this method and compare the effects of different reference drugs upon remission of resultant colon injuries. TNBS was dissolved in 0.25 ml of ethanol (50% v/v) and instilled (25, 50, 100 and 150 mg/kg) intracolonically to the male Wistar rats. After determination of optimum dose of TNBS in male rats and assessment of this dose in female rats, they were treated with reference drugs including dexamethasone [1 mg/kg, intraperitoneally (i.p.) and 2 mg/kg, orally (p.o.)], Asacol (mesalazine, 100 mg/kg, p.o.; 150 mg/kg, enema) and hydrocortisone acetate (20 mg/kg, i.p.; 20 mg/kg, enema) which started 2 h after colitis induction and continued daily for 6 consecutive days. Thereafter, macroscopic and microscopic parameters and clinical features were assessed and compared in different groups. We found that the optimum dose of TNBS for the reproducibility of colonic damage with the least mortality rate was 50 mg/kg. Amongst studied reference drugs, hydrocortisone acetate (i.p.), dexamethasone (i.p. and p.o.) and Asacol (p.o.) significantly diminished the severity of macroscopic and microscopic injuries and could be considered effective for experimental colitis studies in rats . Our findings suggest that optimization of TNBS dose is essential for induction of colitis under the laboratory conditions; and gender exerts no impact upon macroscopic and histological characteristics of TNBS-induced colitis in rats. Furthermore, the enema forms of hydrocortisone and Asacol are not appropriate reference drugs.
Bacteria Foraging Reinforcement Learning for Risk-Based Economic Dispatch via Knowledge Transfer

This paper proposes a novel bacteria foraging reinforcement learning with knowledge transfer method for risk-based economic dispatch, in which the economic dispatch is integrated with risk assessment theory to represent the uncertainties of active power demand and contingencies during power system operations. Moreover, a multi-agent collaboration is employed to accelerate the convergence of knowledge matrix, which is decomposed into several lower dimension sub-matrices via a knowledge extension, thus the curse of dimension can be effectively avoided. Besides, the convergence rate of bacteria foraging reinforcement learning is increased dramatically through a knowledge transfer after obtaining the optimal knowledge matrices of source tasks in pre-learning. The performance of bacteria foraging reinforcement learning has been thoroughly evaluated on IEEE RTS-79 system. Simulation results demonstrate that it can outperform conventional artificial intelligence algorithms in terms of global convergence and convergence rate.
Pediatric and Adult Hepatic Embryonal Sarcoma: A Comparative Ultrastructural Study with Morphologic Correlations

Hepatic embryonal (undifferentiated) sarcoma (ES) is a rare pediatric tumor occurring predominantly in the first decade of life, but a few examples of adult ES have also been described. Isolated ultrastructural reports describe contradictory lines of differentiation in these tumors. Four pediatric and 3 adult ES cases were studied ultrastructurally and features were correlated with morphology. Morphologically, tumors were composed of mixture of plump spindle cells and bizarre giant cells, showing abundant cytoplasmic eosinophilic globules. Ultrastructurally, the hallmark features in all cases included dilated RERs and secondary lysosomes with dense precipitates. Dilated mitochondria and mitochondrial–RER complexes were often seen. Other features included intracytoplasmic fat droplets, scant actin microfilaments, and focal glycogen pools. In summary, pediatric and adult ES show similar morphologic and ultrastructural features. Ultrastructurally, hepatic ES have distinctive findings, including dilated RER and electron-dense lysosomal precipitates, which correlate with the eosinophilic hyaline bodies seen microscopically. These findings suggest that ES are composed of fibroblastic, fibrohistiocytic, and undifferentiated cells. Other lines of differentiation were not identified.
Eliminating incident subtraction in diffraction tomography

Diffraction tomography is a powerful algorithm for producing high-resolution quantitative reconstructions across a wide range of applications. A major drawback of the method is that it operates on the scattered field, which cannot generally be directly measured, but must instead be calculated by subtracting the incident field, i.e. the equivalent field with no scatterer present. Unfortunately, often the incident field is not measurable and hence must be estimated, causing errors. This paper highlights an important, but not widely recognized, result: for particular widely used formulations of the algorithm, the subtraction of the incident field is unnecessary, and the algorithm can actually be applied directly to measured signals. The theory behind this is derived, showing that the incident field will vanish under far-field conditions, and the result is demonstrated in practice. Tests with subsampled arrays show that aliasing artefacts can appear, but can be removed with a filter at the expense of resolution. The incident field also has no effect for a variety of array configurations tested. Finally, the performance in the presence of both correlated and uncorrelated errors is confirmed, in all cases demonstrating that the incident field has a negligible effect on the final reconstruction.
Time Consistency: Stationarity and Time Invariance

A sequence of experiments documents static and dynamic ``preference reversals'' between sooner-smaller and later-larger rewards, when the sooner reward could be immediate. The theoretically-motivated design permits separate identification of time-consistent, stationary and time-invariant choices. At least half of the subjects are time consistent, but only three-quarters of them exhibit stationary choices. About half of subjects with time inconsistent choices have stationary preferences. These results challenge the view that present-bias preferences are the main source of time inconsistent choices.
Genetic variation in the lipoxygenase pathway and risk of colorectal neoplasia

Arachidonate lipoxygenase (ALOX) enzymes metabolize arachidonic acid to generate potent inflammatory mediators and play an important role in inflammation‐associated diseases. We investigated associations between colorectal cancer risk and polymorphisms in ALOX5, FLAP, ALOX12, and ALOX15, and their interactions with nonsteroidal anti‐inflammatory drug (NSAID) use. We genotyped fifty tagSNPs, one candidate SNP, and two functional promoter variable nucleotide tandem repeat (VNTR) polymorphisms in three US population‐based case‐control studies of colon cancer (1,424 cases/1,780 controls), rectal cancer (583 cases/775 controls), and colorectal adenomas (485 cases/578 controls). Individuals with variant genotypes of the ALOX5 VNTR had a decreased risk of rectal cancer, with the strongest association seen for individuals with one or more alleles of >5 repeats (wild type = 5, OR>5/≥5 = 0.42, 95% CI 0.20–0.92; P = 0.01). Four SNPs in FLAP (rs17239025), ALOX12 (rs2073438), and ALOX15 (rs4796535 and rs2619112) were associated with rectal cancer risk at P ≤ 0.05. One SNP in FLAP (rs12429692) was associated with adenoma risk. A false discovery rate (FDR) was applied to account for false positives due to multiple testing; the ALOX15 associations were noteworthy at 25% FDR. Colorectal neoplasia risk appeared to be modified by NSAID use in individuals with variant alleles in FLAP and ALOX15. One noteworthy interaction (25% FDR) was observed for rectal cancer. Genetic variability in ALOXs may affect risk of colorectal neoplasia, particularly for rectal cancer. Additionally, genetic variability in FLAP and ALOX15 may modify the protective effect of NSAID use against colorectal neoplasia. © 2013 Wiley Periodicals, Inc.
Application of Bayesian network model in explaining occupational accidents in a steel industry

In the occupational accident analysis, identification of the interrelationships of the factors behind the accidents is very important. To explore the relationships or the impacts of the causal factors on the accidents and to predict the incident outcomes i.e., injury, near miss, and property damage cases, Bayesian Network (BN) model is used in this paper. The proposed model is validated using the data retrieved from an integrated steel manufacturing industry in India using sensitivity analysis. BN performs well in terms of prediction with 88.28% accuracy using 10-fold cross validation. In addition, some important key findings are obtained from the analysis like the factors slip-trip-falls, crane dashing, and the months February and July are found to be the sensitive factors towards incident outcomes in the plant. The proposed model, therefore, has a good potential for explaining accident prediction and causation in manufacturing industry and can be applied in different domains also.
Mechanism of autocrine stimulation in hematopoietic cells producing interleukin-3 after retrovirus-mediated gene transfer

Endogenous expression of the interleukin-3 (IL3) gene introduced with a retrovirus vector renders hematopoietic cells autonomous of exogenous growth factor. To investigate the mechanism of autocrine stimulation, 25 clones were isolated after retrovirus transduction of IL3 into 32D-cl23 or FDC-P1 cells. Medium conditioned by these autonomous IL3-producing clones supported the growth of factor-dependent 32D cells. Although there was a severalfold variation in the amount of IL3 secreted (some clones secreted barely detectable levels), the doubling time of each clone in the absence of added IL3 was identical to that of the parental cell line maximally stimulated by exogenous IL3. Concentrated monoclonal and polyclonal antibodies, both highly effective in neutralizing exogenous IL3, were assayed for ability to inhibit autocrine growth. Minimal inhibitory effects were observed only on washed autocrine clones secreting low levels of IL3. To test the activity of cytoplasmically synthesized IL3, the nucleotides encoding the signal sequence of IL3 were deleted and replaced with an in-frame ATG in the context of a consensus translation initiation sequence. Ten 32D clones expressing this restructured IL3 genome were obtained. Despite the presence of biologically active IL3 in cell lysates, all clones remained dependent on exogenous IL3, with the same dose-response as that found for 32D cells. Our data are most compatible with a mechanism whereby endogenously produced IL3 interacts with its receptor prior to surface display.
Buried waters and internal cavities in monomeric proteins

We have analyzed the buried water molecules and internal cavities in a set of 75 high‐resolution, nonhomologous, monomeric protein structures. The number of hydrogen bonds formed between each water molecule and the protein varies from 0 to 4, with 3 being most common. Nearly half of the water molecules are found in pairs or larger clusters. Approximately 90% are shown to be associated with large cavities within the protein, as determined by a novel program, PRO_ACT. The total volume of a protein's large cavities is proportional to its molecular weight and is not dependent on structural class. The largest cavities in proteins are generally elongated rather than globular. There are many more empty cavities than hydrated cavities. The likelihood of a cavity being occupied by a water molecule increases with cavity size and the number of available hydrogen bond partners, with each additional partner typically stabilizing the occupied state by 0.6 kcal/mol.
Work environments for employee creativity

Innovative organisations need creative employees who generate new ideas for product or process innovation. This paper presents a conceptual framework for the effect of personal, social-organisational and physical factors on employee creativity. Based on this framework, an instrument to analyse the extent to which the work environment enhances creativity is developed. This instrument was applied to a sample of 409 employees and support was found for the hypothesis that a creative work environment enhances creative performance. This paper illustrates how the instrument can be used in companies to select and implement improvements. Statement of Relevance:The ergonomics discipline addresses the work environment mainly for improving health and safety and sometimes productivity and quality. This paper opens a new area for ergonomics: designing work environments for enhancing employee creativity in order to strengthen an organisation's capability for product and process innovation and, consequently, its competitiveness.
Protein Kinase C in Cardiac Disease and as a Potential Therapeutic Target

Protein kinase C (PKC) is a member of a large family of serine/threonine kinases that plays an integral role in many of the signaling cascades that govern cellular behavior. As such, it is intricately involved in the processes that mediate disease pathogenesis. Strategies that serve to alter PKC function may prove to be useful in the treatment of numerous disease states. This article reviews the various roles PKC may play in cardiovascular disease, specifically with regard to ischemic heart disease, cardiac hypertrophy, heart failure, hypertension, and atherosclerosis, and suggests the potential for developing therapeutic approaches that can target PKC activity.
Effect of Eccentric Exercise Program for Early Tibialis Posterior Tendinopathy

Background: Morphology and vascularization of painful tibialis posterior (TP) tendons before and after an intervention targeting the degenerated tendon were examined. Functional status and pain level were also assessed. Materials and Methods: A10-week twice daily, progressive eccentric tendon loading, calf stretching program with orthoses was implemented with ten, early stage TP tendinopathy subjects. TP tendons were imaged by grayscale and Doppler ultrasound at INITIAL and POST evaluations to assess the tendon's morphology and signs of neovascularization. The Foot Functional Index (FFI), Physical Activity Scale (PAS), 5-Minute Walk Test, and single heel raise (SHR) test were completed at INITIAL and POST evaluations. The Global Rating Scale (GRS) was completed at 6 months followup. One-way ANOVA was used to compare the FFI at INITIAL, POST, and 6-MONTH time points. Paired t-tests were used to compare means between the remaining variables. The level of significance was p = 0.05. Results: There was a significant difference in FFI total, pain, and disability at the three time-points. Post-hoc paired t-tests revealed that the FFI scores were lower for the total score and pain and disability subcategories when comparing from INITIAL to POST and INITIAL to 6-MONTH evaluations (p < 0.05 for all). The number of SHR increased significantly on the involved side from INITIAL to POST evaluation (p = 0.041). The GRS demonstrated minimum clinically important differences for improvements in symptoms at 6-MONTH. Tendon morphology and vascularization remained abnormal following the intervention. Conclusion: A 10-week tendon specific eccentric program resulted in improvements in symptoms and function without changes in tendon morphology or neovascularization. Level of Evidence: IV, Case Series
Validation of the actual behaviour of a real-time application

Classically, the temporal validation of a hard real-time application is performed using the WCET's, for instance by means of simulation. But since the actual behaviour of the application uses the ACET's which may be shorter than the WCET's, the simulated and the effective behaviours may be different. Verifying whether an application behaves in accordance to a given scheduling strategy requires to precisely specify how the application is expected to behave in the case of ACET's shorter than the WCET's. For that aim, we define two notions of compliance. The non-flexible compliance imposes the strict respect of the start times given by the simulated schedule, whereas the flexible one tolerates a higher level of conservatism. We then discuss the advantages of each, and show that using any of them preserves the validity of the behaviour of the application.
Rise in maternal mortality in the Netherlands

Please cite this paper as: Schutte J, Steegers E, Schuitemaker N, Santema J, de Boer K, Pel M, Vermeulen G, Visser W, van Roosmalen J, the Netherlands Maternal Mortality Committee. Rise in maternal mortality in the Netherlands. BJOG 2009;117:399–406.
Juvenile hormone: the status of its "status quo" action.

Juvenile hormone (JH) allows larval molting in response to ecdysteroids but prevents the switching of gene expression necessary for metamorphosis. I first review our efforts to isolate the nuclear receptor for JH in the larval epidermis of Manduca sexta using photoaffinity analogs and our recent findings that the molecule isolated does not bind JH I with high affinity. The reported apparent high affinity binding of JH I by the recombinant 29 kDa protein (rJP29) was artifactual due to the presence of contaminating esterases. Purified rJP29 bound little detectable JH I, but its binding of the photoaffinity analog was prevented by JH I as well as other isoprenoids, indicating a low affinity for these compounds. Our recent studies focus on the effects of JH on the early molecular events induced by 20-hydroxyecdysone (20E). Culture of day 2 5th larval epidermis with 10(-6)M 20E for 24 h caused first pupal commitment, then the onset of the predifferentiative events necessary for pupation. Biphasic increases in the mRNAs of the two isoforms of the ecdysone receptor (EcR-A and EcR-B1) and of E75A, an ecdysteroid-induced transcription factor, coincided with these two phases. The mRNAs for Ultraspiracle (USP) and the metamorphosis-specific Broad-Complex (BR-C) increased only during the second phase. The presence of JH had no effect on the initial increases in EcR mRNAs but caused an increased accumulation of E75A mRNA. This JH also prevented the later changes in EcR, USP, and BR-C mRNAs. Thus, JH influences only certain of the early actions of 20E which then result in its preservation of the "status quo. "
Extended lymph node dissection for gastric cancer: who may benefit? Final results of the randomized Dutch gastric cancer group trial.

PURPOSE The extent of lymph node dissection appropriate for gastric cancer is still under debate. We have conducted a randomized trial to compare the results of a limited (D1) and extended (D2) lymph node dissection in terms of morbidity, mortality, long-term survival and cumulative risk of relapse. We have reviewed the results of our trial after follow-up of more than 10 years. PATIENTS AND METHODS Between August 1989 and June 1993, 1,078 patients with gastric adenocarcinoma were randomly assigned to undergo a D1 or D2 lymph node dissection. Data were collected prospectively, and patients were followed for more than 10 years. RESULTS A total of 711 patients (380 in the D1 group and 331 in the D2 group) were treated with curative intent. Morbidity (25% v 43%; P <.001) and mortality (4% v 10%; P =.004) were significantly higher in the D2 dissection group. After 11 years there is no overall difference in survival (30% v 35%; P =.53). Of all subgroups analyzed, only patients with N2 disease may benefit of a D2 dissection. The relative risk ratio for morbidity and mortality is significantly higher than one for D2 dissections, splenectomy, pancreatectomy, and age older than 70 years. CONCLUSION Overall, extended lymph node dissection as defined in this study generated no long-term survival benefit. The associated higher postoperative mortality offsets its long-term effect in survival. For patients with N2 disease an extended lymph node dissection may offer cure, but it remains difficult to identify patients who have N2 disease. Morbidity and mortality are greatly influenced by the extent of lymph node dissection, pancreatectomy, splenectomy and age. Extended lymph node dissections may be of benefit if morbidity and mortality can be avoided.
Epidemiological features of HTLV‐I carriers and incidence of ATL in an ATL‐endemic island: A report of the community‐based co‐operative study in Tsushima, Japan

To extend the epidemiological study on adult T‐cell leukemia‐lymphoma (ATL) in japan, the geographical and demographic characteristics of carriers of human T‐lymphotropic virus type I (HTLV‐I) and patients with ATL were analyzed in Tsushima Island, which is one of the typical endemic areas of ATL in Kyushu, japan. Even on the small island of Tsushima (710 km2; pop. 48,875; 123 villages), the positive rates of anti‐HTLV‐1 antibody among the 58 villages studied varied from 2% to 50%, a fact that is probably associated with the historical events affecting the movement of the indigenous population of Tsushima Island. The positive rate of anti‐HTLV‐1 antibody in males increased little with age; however, the female rate increased distinctly with age in moderate and high HTLV‐1‐endemic villages where more than 15% of the inhabitants had positive anti‐HTLV‐1 antibody. Analysis of anti‐HTLV‐1 antibody positivity between spouses confirmed that HTLV‐1 was more contagious from husband to wife than from wife to husband, which corresponded to the fact that the positive rate of anti‐HTLV‐1 antibody in females older than 30 years was higher than that in males. Recently the rate of carrier children from HTLV‐1 carrier mothers was estimated at around 20%. The recent annual incidence rates of ATL among 1,000 HTLV‐1 carriers older than 40 years living in Tsushima Island was estimated at 2.2 in males, 0.8 in females.
Response of White Leghorn Chickens of Various B Haplotypes to Infection at Hatch with Subgroup J Avian Leukosis Virus

Abstract White leghorn chickens from seven 15.B congenic lines (genetically similar except for genes linked to the major histocompatibility complex [MHC] B haplotype) and two Line 0.B semicongenic lines were infected at hatch with strain ADOL Hc-1 of subgroup J avian leukosis virus (ALV-J). At 5, 8, 16, and 36 wk of age, chickens were tested for viremia, serum-neutralizing antibody, and cloacal shedding. Chickens were also monitored for development of neoplasia. In the 15.B congenic lines (B*2, B*5, B*12, B*13, B*15, B*19, and B*21) there were no significant differences in the incidence of viremia between B haplotypes. In fact, infection at hatch in all of the 15.B congenic lines induced tolerance to ALV-J because 100% of these chickens were viremic and transient circulating serum-neutralizing antibody was detected in only a few chickens throughout the 36 wk experiment. However, at 16 wk of age more B*15 chickens had antibody and fewer B*15 chickens shed virus than did the 16-wk-old B*2, B*5, or B*13 chickens. Moreover, compared with B*15 chickens, a higher percentage of B*13 chickens consistently shed virus from 8 wk postinfection to termination at 36 wk postinfection. The B haplotype had a transient effect on viral clearance in Line 0.B semicongenics, as more B*13 than B*21 chickens remained viremic through 5 wk of age. Very few (0%–18%) of the Line 0.B semicongenic chickens shed virus. By 36 wk of age, all Line 0 B*13 and B*21 chickens produced serum-neutralizing antibodies and cleared the virus. These results show that following ALV-J infection at hatch the immune response is influenced transiently by the B haplotype and strongly by the line of chicken. Although this study was not designed to study the effect of endogenous virus on ALV-J infection, the data suggest that endogenous virus expression reduced immunity to ALV-J in Line 15I5, compared with Line 0, a line known to lack endogenous virus genes.