Miracle-dz/UniFAR-FTUs huggingface dataset:
Successful isolation and expansion of CMV‐reactive T cells from G‐CSF mobilized donors that retain a strong cytotoxic effector function

Cytomegalovirus (CMV) infections post‐haematopoietic stem cell transplantation (HSCT) can be effectively controlled through the adoptive transfer of donor‐derived CMV‐specific T cells (CMV‐T). Current strategies involve a second leukapheresis collection from the original donor to manufacture CMV‐T, which is often not possible in the unrelated donor setting. To overcome these limitations we have investigated the use of a small aliquot of the original granulocyte‐colony stimulating factor (G‐CSF) mobilized HSCT graft to manufacture CMV‐T. We explored the T cell response to CMVpp65 peptide stimulation in G‐CSF mobilized peripheral blood mononuclear cells (PBMC) and subsequently examined isolation of CMV‐T based on the activation markers CD154 and CD25. CD25+ enriched CMV‐T from G‐CSF mobilized PBMC contained a higher proportion of FoxP3 expression than non‐mobilized PBMC and showed superior suppression of T cell proliferation. Expanded CMV‐T enriched through CD154 were CD4+ and CD8+, demonstrated a high specificity for CMV, secreted cytotoxic effector molecules and lysed CMVpp65 peptide‐loaded phytohaemagglutinin‐stimulated blasts. These data provide the first known evidence that CMV‐T can be effectively manufactured from G‐CSF mobilized PBMC and that they share the same characteristics as CMV‐T isolated in an identical manner from conventional non‐mobilized PBMC. This provides a novel strategy for adoptive immunotherapy that abrogates the need for successive donation.
What Changes the Rules of the Game in Wholly Owned Subsidiaries? Determinants of Knowledge Acquisition from Parent Firms

We examine key factors affecting the extent of knowledge acquisition from multinational enterprises (MNEs) in their wholly owned subsidiaries (WOSs). As the volume of foreign direct investment (FDI) by MNEs is rapidly growing, empirical studies dealing with knowledge acquisition from parent firms in subsidiaries are in the limelight. However, as far as we know, noone has attempted to identify primary mechanisms influencing subsidiary learning by dividing WOSs based on "investment mode" and "investment direction". We believe WOSs are characterized by these two issues and thus this research contributes to current literature by providing a detailed picture of learning mechanisms in subsidiaries. We advance a series of propositions to achieve the research objective by using a sample of WOSs established by MNEs in Korea. By doing this, we reveal that subsidiary learning depends significantly on absorptive capacity in learning organizations, relational capital and parent firms" behavior. Also, we confirm that factors facilitating knowledge acquisition are influenced by investment mode and investment direction. Based on the results, this study provides some useful implications to MNEs and policy makers in local markets.
The Social Entrepreneurship Zone

Social entrepreneurship is still in the developmental stage as a field of inquiry. In this article we introduce the concept of the social entrepreneurship zone. This new construct positions social entrepreneurship relative to the ways organizations plan to implement social change and the degrees to which they apply business practices to do so. Two new categories of organizations, social transformation entrepreneurial ventures and social improvement entrepreneurial ventures, reside in the social entrepreneurship zone. This categorization separates social entrepreneurial ventures into 2 distinct groups with their own unique set of characteristics. The social entrepreneurship zone construct should help researchers and other stakeholders better understand this emerging field so it can be further studied and developed.
On Robust Concepts and Small Neural Nets

The universal approximation theorem for neural networks says that any reasonable function is well-approximated by a two-layer neural network with sigmoid gates but it does not provide good bounds on the number of hidden-layer nodes or the weights. However, robust concepts often have small neural networks in practice. We show an efficient analog of the universal approximation theorem on the boolean hypercube in this context. We prove that any noise-stable boolean function on n boolean-valued input variables can be well-approximated by a two-layer linear threshold circuit with a small number of hidden-layer nodes and small weights, that depend only on the noisestability and approximation parameters, and are independent of n. We also give a polynomial time learning algorithm that outputs a small two-layer linear threshold circuit that approximates such a given function. We also show weaker generalizations of this to noise-stable polynomial threshold functions and noise-stable boolean functions in general.
Effect of Membrane Polyunsaturation on Carrier‐Mediated Transport in Cultured Retinoblastoma Cells: Alterations in Taurine Uptake

Summary: Neural cell membranes naturally contain a large amount of polyunsaturated fatty acid, but the functional significance of this is unknown. An increase in membrane polyunsaturation has been shown previously to affect the high‐affinity transport systems for choline and glycine in cultured human Y79 retinoblastoma cells. To test the generality of membrane polyunsaturation effects on transport, we investigated the uptake of other putative neurotransmitters and amino acids by these cells. Taurine, glutamate, and leucine were taken up by both high‐ and low‐affinity transport systems, whereas serine, γ‐aminobutyrate, and α‐aminoisobutyrate were taken up only by low‐affinity systems. The high‐affinity taurine and glutamate and low‐affinity serine uptake systems were Na+ dependent. Arachidonic acid (20:4) supplementation of Y79 cells produced enrichment of all the major microsomal phosphoglycerides with 20:4, while docosahexaenoic acid (22:6) supplementation produced large increases in the 22:6 content of all fractions except the inositol phosphoglycerides. Enrichment with these polyunsaturated fatty acids facilitated taurine uptake by lowering the K′ of its high‐affinity transport system. By contrast, enrichment with oleic acid did not affect taurine uptake. Glutamate, leucine, serine, γ‐aminobutyrate, and α‐aminoisobutyrate uptake were not affected when the cells were enriched with any of these fatty acids. These findings demonstrate that only certain transport systems are sensitive to the polyunsaturated fatty acid content of the retinoblastoma cell membrane. The various transport systems either respond differently to changes in membrane lipid unsaturation, or they are located in lipid domains that are modified to different extents by changes in unsaturation.
Integrating Competitive Strategy and Transaction Cost Economics: An Operationalization of Fit in the Interstate Trucking Industry

While Porter's competitive strategy framework and Williamson's transaction cost economics have had an immense impact on modern strategic management research, they have tended to talk past each other. This paper provides an explicit integration of competitive strategy and transaction cost economics to explain the existence of different organizational forms within the same industry. We propose that a firm's strategic positioning choice has far-reaching implications for the assets it needs to assemble and the hazards to which its assets are exposed. A firm's organizational structure should "fit" its strategic position, which is characterized by the customer needs it serves and the technology and asset portfolio it employs, in that it adopts a transaction cost economizing form of organization given its strategic position. An analysis of the employment relation in the trucking industry provides empirical support for our predictions.
Money Market Funds and Financial Stability

The financial crisis, in particular the collapse of Lehman Brothers, has revealed that money market funds are more risky than had previously been believed. We discuss the importance of money market funds for financial stability and whether situations similar to those in the US and Icelandic markets could arise in Sweden. We find that there are similarities between the Swedish and Icelandic funds, but few similarities with the US funds. In Sweden, as was the case in Iceland, the assets under management are concentrated in a few funds and the connection to the major banks is strong. However, given the relatively small size of the money market funds in Sweden, we do not find that they, in isolation, are of major systemic importance as a source of funding for the Swedish banks. The funds are more likely to have a systemic impact through spill-over effects on the banking system, especially in a market already characterised by high uncertainty and risk aversion. The money market funds are thus more important to certain parts of the financial market, such as the market for corporate commercial paper and covered bonds.
A Bi-level Programming Models in the Optimization for the Position and Scale of Logistics Points

The planning and layout of the logistics points not only influences the business activities of enterprises, but also influences the traffic functions of the city. With the benefits of both users and logistics planners taken into consideration, a Bi-level programming model is proposed in this paper to describe the optimization for the position and scale of logistics points. The upper level problem is to minimize the generalized logistics' total cost from the terms of decision makers by taking account of the relationships of the logistics points layout, the transport cost and the storage cost. The lower level describes the user's choice behavior, corresponding to the biggest utility choice the logistics points, and corresponding to the traffic condition choice transportation route. And this model solution algorithm is designed.
An Integrated Active-RC Powerline Notch Filter for Biopotential Acquisition Devices

An integrable 60Hz continuous time active-RC notch filter is presented. This is made possible through replacing passive resistors by R-2R ladders providing area saving of approximately 120 times. The proposed filter is to be embedded into instrumentation amplifier for biopotential measuring devices. Simulation results of a fully differential 4-order filter show notch depth of 68dB and THD of better than -70dB while consuming 18μW.
The Effect Of Accounting Regime Characteristics On The Prediction Of Future Cash Flows: An International Comparison

For nine countries, we show that the components of accrual accounting earnings provide information incremental to that of current cash flows from operations in explaining next year’s cash flows from operations. We relate the usefulness of accounting earnings components for explaining near-term cash flows to certain country characteristics—common/code-law jurisdiction, accrual index, shareholders’ rights, and uncertainty avoidance. We provide evidence that accounting accruals generated by shorter horizon, code-law regimes provide more incremental explanatory power for short-term predictions than those of longer horizon, commonlaw countries.
A detecting method of electromagnetic emanations based on virtual instrument

Digital electronic equipment emitting unintentional electromagnetic signals in working state need to be tested to ensure data security. The ordinary testing method using traditional devices, such as spectrum analyzer and oscilloscope, cannot provide multi-functions and it is inconvenient outside the laboratory. The novel testing method using virtual instrument can provide more portability and flexibility and the leakage signal reconstruction effect is more intuitive.
CCMpred — Supplementary Information

1 Markov Random Field Model The following section will outline the mathematical model in our contact prediction method that is essentially identical to the plmDCA (Ekeberg et al., 2013) and GREMLIN (Kamisetty et al., 2013) methods. We eliminate transitive interactions in the observed interaction network by learning a generative model of the MSA using a Markov Random Field (MRF). Assuming we have an MSA xi (n sequence index, i position index) with L columns and N sequences, we represent columns in the MSA as the vertices with single-residue emission potentials εi(a) (with i being a column index and a ∈ {1.20} representing the twenty possible amino acids) and covariation between columns as the edges with pairwise emission potentials εi,j(a, b) (with i and j column indices, a and b amino acid indices). The network of co-evolution can then be learned by maximizing the likelihood of observing the sequences in the input MSA, given the model parameters ε:
Iron deficiency: A concise review

Iron deficiency is a major worldwide health problem. There is recent evidence that the anemia is only the last manifestation of the syndrome and that symptoms occur before the anemia is manifest. Advances in outlining the physiology of iron deficiency have been made, gaps remain in the current understanding. While oral iron supplement remains the mainstay, some indications for the intravenous administration have developed. This review will highlight the epidemiology, physiology, clinical presentation, and treatment options. Am. J. Hematol. 78:225–231, 2005. © 2005 Wiley‐Liss, Inc.
Transient inward current underlying arrhythmogenic effects of cardiotonic steroids in Purkinje fibres.

1. Voltage‐clamp experiments were carried out in calf Purkinje fibres to determine the basis of transient depolarizations (TDs) associated with digitalis‐induced arrhythmias. 2. Under the influence of strophanthidin, depolarizing clamp pulses were followed by a transient inward current (TI) which was small or absent in untreated preparations. The TI also appeared in the wake of a train of action potentials. 8. The TI can help generate spontaneous depolarizations in preparations showing the low voltage oscillations which often occur with advanced digitalis toxicity. 8. The TI can help generate spontaneous depolarizations in preparations showing the 'low voltage oscillation' which often occur with advanced digitais toxicity. It was designated TI because its magnitude and timing were appropriate to account for the TD. 3. Longitudinal voltage non‐uniformity during the TI was determined with two voltage‐recording micro‐electrodes. Although the non‐uniformity was not severe, the TI wave form was observed when the voltage difference signal was used to measure membrane current density. 4. Over the diastolic range of potential, the strophanthidin‐induced TI appeared superimposed upon the normal pace‐maker mechanism, the decay of a potassium current, iK2. The TI could be dissociated from iK2, however, by means of its unusual kinetic properties. 5. TIs could also be recorded at holding potentials positive to ‐55 mV, i.e. outside the range where iK2 deactivation occurs. 6. The TI amplitude showed a slow and strongly sigmoid dependence on the duration of the preceding depolarizing pulse. Stronger depolarizing reduced the TI amplitude, while slowing and exaggerating the sigmoid time‐dependence. 7. Two clamp pulses in close succession gave additive effects in evoking a subsequent TI. This finding and the sigmoid time‐dependence fit with previous observations that TDs are most prominent following a series of closely spaced action potentials.
Feasibility analysis of a Global Logistics Hub in Panama

The objective of this essay is to identify the more relevant criteria for a Global Logistic Hub (GLH) development in Panama, which main obstacle is the multi-criteria evaluation and decision environment it is immersed in. The Analytic Hierarchy Process (AHP) was chosen because of its capacity to deal with such complex decision environment. After identification of Panama’s logistic potential, the next step was surveying the development criteria within a widely group of specialists and applying the AHP technical aspects. The results reveal global strategic position, Vorland development and proximity to import/export areas as the more relevant criteria to assure a GLH development.
Choice of Supplier in Embedded Markets: Relationship and Marketing Program Effects

Recent research has documented how exchanges between buyers and sellers are frequently embedded in social relationships. An unresolved question, however, is the extent to which such relationships protect incumbent suppliers from new competitors and their marketing programs. The authors develop a conceptual framework of how relationship and marketing variables influence choice of supplier and test the framework empirically in the context of business-to-business services. The results show that interpersonal relationships between buyers and suppliers serve as a switching barrier but are considerably less important than both firm-level switching costs and marketing variables. Moreover, unlike switching costs, interpersonal relationships do not play the frequently mentioned role of a buffer against price and product competition. Finally, the authors show that buyers and suppliers hold systematically different views of the determinants of switching.
Get Ready for Innovation by Invasion

From many years of field research in West Germany and the United States, and especially from a recent study of relatively mature firms in Ohio, Gerhard Mensch has become aware of the disruption caused by the invasion of industries by new technologies from the outside. He sees the frequency of these invasions increasing in coming years for reasons that go beyond technological matters to the pressures for change coming from the capital markets. In this Perspective, he suggests the invasions can be countered and the capital markets satisfied by giving a more prominent role to the innovation manager in the strategy development process.
T Cell Antigen Receptor Ubiquitination Is a Consequence of Receptor-mediated Tyrosine Kinase Activation (*)

Engagement of the T cell antigen receptor results in both its phosphorylation and its ubiquitination. T cell antigen receptor ubiquitination was evaluated in Jurkat, a well characterized human T leukemia cell line. Treatment of cells with the tyrosine kinase inhibitor herbimycin A resulted in an inhibition of receptor ubiquitination. Consistent with this, pervanadate, which increases cellular tyrosine phosphorylation, enhanced receptor ubiquitination. A requirement for receptor-mediated tyrosine kinase activity for ubiquitination was confirmed in cells lacking the tyrosine kinase p56 and also in cells that are defective in expression of CD45, a tyrosine phosphatase that regulates the activity of p56. The need for tyrosine kinase activation for ubiquitination was not bypassed by directly activating protein kinase C and stimulating endocytosis of receptors. These observations establish ubiquitination of the T cell antigen receptor as a tyrosine kinase-dependent manifestation of transmembrane signaling and suggest a role for tyrosine phosphorylation in the ligand-dependent ubiquitination of mammalian transmembrane receptors.
Workers Compensation Insurance: Incentive Effects of Experience Rating on Work-related Health and Safety

This article examines Workers Compensation Insurance experience rating premiums setting, a common financial incentive tool existing in several countries. Premiums paid by firms are experience rated, which may encourage them to reduce work-related injuries and disabilities. This article provides a literature review on effects of experience rating on work-related health and safety, and empirical results on the French jurisdiction, using sectorial data from industry and construction sectors in 2005. Results are consistent with the hypothesis that this policy tool is a lever that contributes to improve working conditions and reduce work-related injuries rates.
A longitudinal study of the impact of market structure, firm structure, strategy, and market orientation culture on dimensions of small-firm performance

The purpose of this article is to determine market orientation’s relative impact on small-business performance, compared to other influences, in an integrated model using longitudinal data. Contrary to expectations based on the management literature, the results indicate weak causal relationships between market environment, small-firm structure, and small-firm strategy. The results further indicate weak influences of these variables, but strong and consistent influences of market orientation, on various measures of small-firm performance. Contrary to expectations based on business policy literature, relative product quality and new product success were not significant influences on profitability, perhaps due to the significant influence of market orientation on these variables. In addition, although increases in growth/share had a significant short-term influence on increases in profitability, high levels of previous years’ firm growth/share had a negative influence on current profitability. The previous year’s level of firm coordinating systems and market competitive intensity has a significant impact on the level of small-firm market orientation.
Carbon nanotube – polymer composites for photonic devices

We report the fabrication of high optical quality single wall carbon nanotube polyvinyl alcohol composites and their application in nanotube based photonic devices. These show a broad absorption of semiconductor tubes centred at 1.55mm, the spectral range of interest for optical communications. The films are used as mode-lockers in an erbium doped fibre laser, achieving 700 fs mode-locked pulses. Raman spectroscopy shows no damage after a long time continuous laser operation. r 2006 Elsevier B.V. All rights reserved. PACS: 42.55. Wd; 42.60. Fc; 78.67. Ch
LEADERSHIP STYLE AND ORGANIZATIONAL PERFORMANCE IN FAMILY OWNED SMALL SCALE MANUFACTURING INDUSTRIES IN SRI LANKA

Leadership in a family business is a far different challenge than leadership in any other kind of business. The leadership in the family business is aimed at helping business-owning families understand and implement the extra dimensions of leadership that family ﬁ rm require. This study explores the leadership style and organizational performance in small scale manufacturing industries in Sri Lanka. This study utilized data from 220 small scale manufacturing industries those who are registered under the Chambers of Commerce in Sri Lanka. The relationship between three leadership styles – task-oriented, relationship-oriented, and participative – and ﬁrm performance were discussed through the moderating effect of environmental factors (Industry technology, family and non-family controlled ﬁrm and ﬁrm development stage), and mediating effect of decision making, employee turnover, and employee morale. The hypotheses were tested through matched data set. The results indicated that leadership styles were directly linked to firm performance. Keywords: Leadership Style, Decision making, Employee turnover, Employee Morale, Performance. For full paper: fmscresearch@sjp.ac. lk
A Deep Learning Approach for Cancer Detection and Relevant Gene Identification

Cancer detection from gene expression data continues to pose a challenge due to the high dimensionality and complexity of these data. After decades of research there is still uncertainty in the clinical diagnosis of cancer and the identification of tumor-specific markers. Here we present a deep learning approach to cancer detection, and to the identification of genes critical for the diagnosis of breast cancer. First, we used Stacked Denoising Autoencoder (SDAE) to deeply extract functional features from high dimensional gene expression profiles. Next, we evaluated the performance of the extracted representation through supervised classification models to verify the usefulness of the new features in cancer detection. Lastly, we identified a set of highly interactive genes by analyzing the SDAE connectivity matrices. Our results and analysis illustrate that these highly interactive genes could be useful cancer biomarkers for the detection of breast cancer that deserve further studies.