Miracle-dz/UniFAR-FTUs huggingface dataset:
Ribosomal association of the yeast SAL4 (SUP45) gene product: implications for its role in translation fidelity and termination

The SAL4 gene of the yeast Saccharomyces cerevisiae encodes a novel translation factor (Sal4p) involved in maintaining translational fidelity. Using a polyclonal antibody raised against a Sal4p‐β‐galac‐tosidase fusion protein, Sal4p was shown to be almost exclusively associated with the ribosomal fraction. Even when the ribosomes were treated with 0.8 M KCl, only low levels of Sal4p were detected in the post‐ribosomal supernatant, suggesting a very strong affinity between Sal4p and the ribosome. Analysis of the distribution of Sal4p in the ribosomal population revealed that it was principally associated with 40S subunits, monosomes and polysomes. Incubation in high salt concentrations (0.8 M KCl) suggested that the affinity of Sal4p for the 40S subunit was lower than that for monosomes or polysomes. The Sal4p:ribosome association was only maintained when ribosomes were prepared in the presence of the translation elongation inhibitor cycloheximide; in uninhibited cells much lower levels of Sal4p were detectable in the ‘run‐off’ polysomes. In view of these data, and given the stoichiometry of Sal4p to individual ribosomal proteins (estimated at less than 1:20), we suggest that Sal4p plays an ancillary role in translation termination.
Treating bacteria with bacteria : the role of probiotics in the eradication of Helicobacter pylori

Helicobacter pylori (H. pylori), with more than 50% global population infection, is an etiology of chronic gastritis and peptic ulcer and a risk factor for gastric malignancies. Due to the increase of H. pylori-resistant strains and treatment-related adverse effects, the success rate of eradication therapies has declined to 70%, far below the initial rate of 90%. To improve the eradication rate, researchers have started to look into alternative treatments for H. pylori, one of which is probiotics. In this article, we elaborated the mechanisms of action of probiotics against H. pylori infection into four aspects: immunomodulation, antimicrobial substances, interfering with adhesion, and mucosal barrier. Although probiotics are varied, we only conducted studies on Lactobacillus, Bifidobacterium, and yeasts such as Saccharomyces boulardii most commonly used in the therapy. The effects of Lactobacillus, Bifidobacterium, and Saccharomyces boulardiias monotherapy or adjuvant therapy combined with antibiotic-PPI for H. pylori eradication in children and adults showed diverse outcomes in eradication rate and adverse effects. Extensive studies indicated probiotics plays an important role in H. pylori infection. However, many uncertain problems still need to be considered in the clinical application of probiotics.
Cylindrical Conformal Arrays Loaded With Frequency Selective Screens

The new trends in the communication market are demanding for wider bandwidths and consequently higher frequencies and higher bit rates. The traditional hardware development, based on the manufacture and measurement of test components, is too expensive and time consuming. The availability of novel integrated design tools and manufacturing techniques is therefore an essential requirement. In this paper, we present an accurate and efficient tool for the analysis of multilayer conformal cylindrical structures, based on a Multimode Equivalent Network (MEN) formulation.
Size Effects in Active Superconductor Antennas

Despite the fact that active an electrically small antenna based on Superconducting Quantum Array forms output voltage which is independent of the antenna size to wavelength ratio, a small size effect can appear and limit the antenna output linearity with the ratio increase. We analyze the size effect in the active antennas of both transformer and transformer-free types.
Hearing Impairment and Physical Function and Falls in the Atherosclerosis Risk in Communities Hearing Pilot Study

1. Nakagami T, Qiao Q, Carstensen B et al.; DECODE-DECODA Study Group. Age, body mass index and type 2 diabetes-associations modified by ethnicity. Diabetologia 2003;46:1063–1070. 2. Araneta MR, Kanaya AM, Hsu WC et al. Optimum BMI cut points to screen Asian Americans for type 2 diabetes. Diabetes Care 2015;38:814– 820. 3. Florez H, Ruiz J, Munshi M. Aging and diabetes mellitus. In: De Fronzo RA, Ferrannini E, Zimmet P et al., eds. International Textbook of Diabetes Mellitus, 4th Ed. New York: Wiley-Blackwell, 2015, pp 836–845. 4. Aizawa T, Yamada M, Katakura M et al. Hyperbolic correlation between insulin sensitivity and insulin secretion fades away in lean subjects with superb glucose regulation. Endocr J 2012;59:127–136. 5. Examination Committee of Criteria for ‘Obesity Disease’ in Japan; Japan Society for the Study of Obesity. New criteria for ‘obesity disease’ in Japan. Circ J 2002;66:987–992.
Empirical determinants of measles metapopulation dynamics in England and Wales

A key issue in metapopulation dynamics is the relative impact of internal patch dynamics and coupling between patches. This problem can be addressed by analysing large spatiotemporal data sets, recording the local and global dynamics of metapopulations. In this paper, we analyse the dynamics of measles metapopulations in a large spatiotemporal case notification data set, collected during the pre–vaccination era in England and Wales. Specifically, we use generalized linear statistical models to quantify the relative importance of local influences (birth rate and population size) and regional coupling on local epidemic dynamics. Apart from the proportional effect of local population size on case totals, the models indicate patterns of local and regional dynamic influences which depend on the current state of epidemics. Birth rate and geographic coupling are not associated with the size of major epidemics. By contrast, minor epidemics, and especially the incidence of local extinction of infection, are influenced both by birth rate and geographical coupling. Birth rate at a lag of four years provides the best fit, reflecting the delayed recruitment of susceptibles to school cohorts. A hierarchical index of spatial coupling to large centres provides the best spatial model. The model also indicates that minor epidemics and extinction patterns are more strongly influenced by this regional effect than the local impact of birth rate.
An improved projection algorithm for direct adaptive fuzzy control

An effective variant of the projection algorithm frequently used in adaptive fuzzy control is presented. The proposed algorithm preserves the formal guarantees that the adapted parameters and some related system variables remain within the prescribed bounds, and improves the speed of convergence of the adaptation. The effectiveness of our approach is shown both in simulation and experimental case studies.
The Management of Probabilistic Data

It is often desirable to represent in a database, entities whose properties cannot be deterministically classified. The authors develop a data model that includes probabilities associated with the values of the attributes. The notion of missing probabilities is introduced for partially specified probability distributions. This model offers a richer descriptive language allowing the database to more accurately reflect the uncertain real world. Probabilistic analogs to the basic relational operators are defined and their correctness is studied. A set of operators that have no counterpart in conventional relational systems is presented. >
Introgression of low phytic acid locus ('lpa2-2') into an elite Maize ('Zea mays' L.) inbred through marker assisted backcross breeding

Phytic Acid (PA) in maize kernel is an anti-nutritional factor; it chelates mineral cations in human gut and causes mineral deficiency in humans. Therefore, development of low-PA maize is required. Here, we have transferred the low-PA locus (i.e., lpa2-2 allele that confers low-PA trait) by crossing low-PA line “EC 659418” (donor line) with an elite inbred “UMI 285” (recipient line) using Marker Assisted Backcross Breeding (MABB). In our MABB program, for “foreground selection” (i.e., screening for plants with introgressed lpa2-2 locus) small sequence repeat (SSR) marker “umc2230” was used, and for “background selection” (i.e., screening for introgressed lines whose genetic background are similar to that of the recurrent parent) 47 SSR markers were used. As a result, we have developed four lines with both reduced-PA trait similar to that of donor parent, and agronomical traits (i.e., days to 50% tasseling, days to 50% silking, plant height, ear height, 100 seed weight, grain yield per plant, germination and seed vigor) similar to that of the recurrent parent.
Genetic and Molecular Analysis of cglB, a Gene Essential for Single-Cell Gliding in Myxococcus xanthus

ABSTRACT Gliding movements of individual isolated Myxococcus xanthus cells depend on the genes of the A-motility system (agl and cgl genes). Mutants carrying defects in those genes are unable to translocate as isolated cells on solid surfaces. The motility defect of cgl mutants can be transiently restored to wild type by extracellular complementation upon mixing mutant cells with wild-type or other motility mutant cells. To develop a molecular understanding of the function of a Cgl protein in gliding motility, we cloned the cglB wild-type allele by genetic complementation of the mutant phenotype. The nucleotide sequence of a 2.85-kb fragment was determined and shown to encode two complete open reading frames. The CglB protein was determined to be a 416-amino-acid putative lipoprotein with an unusually high cysteine content. The CglB antigen localized to the membrane fraction. The swarming and gliding defects of a constructed ΔcglBmutant were fully restored upon complementation with thecglB wild-type allele. Experiments with a cglBallele encoding a CglB protein with a polyhistidine tag at the C terminus showed that this allele also promoted wild-type levels of swarming and single-cell gliding, but was unable to stimulate ΔcglB cells to move. Possible functions of CglB as a mechanical component or as a signal protein in single cell gliding are discussed.
Physical therapy treatment time during inpatient spinal cord injury rehabilitation

Abstract Background/objective To describe the nature and distribution of activities during physical therapy (PT) delivered in inpatient spinal cord injury (SCI) rehabilitation and discuss predictors (patient and injury characteristics) of the amount of time spent in PT for specific treatment activities. Methods Six hundred patients from six inpatient SCI centers were enrolled in the SCIRehab study. Physical therapists documented details, including time spent, of treatment provided during 37 306 PT sessions that occurred during inpatient SCI rehabilitation. Ordinary least squares regression models associated patient and injury characteristics with time spent in specific PT activities. Results SCIRehab patients received a mean total of 55.3 hours of PT over the course of their rehabilitation stay. Significant differences among four neurologic groups were seen in the amount of time spent on most activities, including the most common PT activities of strengthening exercises, stretching, transfer training, wheelchair mobility training, and gait training. Most PT work (77%) was provided in individual therapy sessions; the remaining 23% was done in group settings. Patient and injury characteristics explained only some of the variations seen in time spent on wheelchair mobility, transfer and bed mobility training, and range of motion/stretching. Conclusion Analysis yielded both expected and unexpected trends in SCI rehabilitation. Significant variation was seen in time spent on PT activities within and among injury groups. Providing therapeutic strengthening treatments consumed the greatest proportion of PT time. About one-quarter of all PT services were provided in group settings. Details about services provided, including time spent, will serve as a starting point in detailing the optimal treatment delivery for maximal outcomes.
Use of Transoesophageal Echocardiography during the Peri-operative Period for Trauma Patients

The medical facility at Camp Bastion continues to evolve as a consequence of the increased throughput of battlefield trauma patients. There is a requirement for rapid and accurate diagnosis of haemodynamic instability and continued haemodynamic monitoring throughout the peri-operative period. Transoesophageal echocardiography (TOE) has been used for this purpose in the arena of cardiac anaesthesia since the mid 1980s. It is being introduced to other peri-operative settings where severe haemodynamic instability is expected. The old proverb: ‘There are none so blind as those who cannot see’ (Jeremiah 5:21) is applicable to this topic, in that TOE is proven to be a rapid, portable, safe and effective tool in the assessment of the haemodynamically unstable patient. This paper explores the application of TOE for the assessment of the major causes of haemodynamic instability in the trauma population.
A hybrid plasmid is a stable cloning vector for the cyanobacterium Anacystis nidulans R2

Anacystis nidulans R2 is a highly transformable strain which is suitable as a recipient for molecular cloning in cyanobacteria. In an effort to produce an appropriate cloning vector, we constructed a hybrid plasmid molecule, pSG111, which contained pBR328 from Escherichia coli and the native pUH24 plasmid of A. nidulans. pSG111 replicated in and conferred ampicillin and chloramphenicol resistance to both hosts. It contained unique sites for the restriction enzymes EcoRI, SalI, SphI, and XhoI, which could be used for the insertion of exogenous DNA. To demonstrate that a molecule like pSG111 could serve as a shuttle vector for the cloning of A. nidulans genes, we constructed a hybrid plasmid, pRNA404, containing an A. nidulans rRNA operon. This recombinant molecule was genetically and structurally stable during passage through A. nidulans and E. coli. The stability of the hybrid plasmid and the inserted rRNA operon demonstrates the feasibility of cloning in A. nidulans with hybrid vectors, with the subsequent retrieval of cloned sequences.
Applications of Bregman Divergence Measures in Bayesian Modeling

This dissertation has mainly focused on the development of statistical theory, methodology, and application from a Bayesian perspective using a general class of divergence measures (or loss functions), called Bregman divergence. Many applications of Bregman divergence have played a key role in recent advances in machine learning. My goal is to turn the spotlight on Bregman divergence and its applications in Bayesian modeling. Since Bregman divergence includes many well-known loss functions such as squared error loss, Kullback-Leibler divergence, Itakura-Saito distance, and Mahalanobis distance, the theoretical and methodological development unify and extend many existing Bayesian methods. The broad applicability of both Bregman divergence and Bayesian approach can handle diverse types of data such as circular data, high-dimensional data, multivariate data and functional data. Furthermore, the developed methods are flexible to be applied to real applications in various scientific fields including biology, physical sciences, and engineering. Applications of Bregman Divergence Measures in Bayesian Modeling
Oncoprotein TLS Interacts with Serine-Arginine Proteins Involved in RNA Splicing*

The gene encoding the human TLS protein, also termed FUS, is located at the site of chromosomal translocations in human leukemias and sarcomas where it forms a chimeric fusion gene with one of several different genes. To identify interacting partners of TLS, we screened a yeast two-hybrid cDNA library constructed from mouse hematopoietic cells using the C-terminal region of TLS in the bait plasmid. Two cDNAs encoding members of the serine-arginine (SR) family of proteins were isolated. The first SR protein is the mouse homolog of human splicing factor SC35, and the second SR member is a novel 183-amino acid protein that we term TASR (TLS-associated serine-arginine protein). cDNA cloning of human TASR indicated that mouse and human TASR have identical amino acid sequences. The interactions between TLS and these two SR proteins were confirmed by co-transfection and immunoprecipitation studies. In vivosplicing assays indicated that SC35 and TASR influence splice site selection of adenovirus E1A pre-mRNA. TLS may recruit SR splicing factors to specific target genes through interaction with its C-terminal region, and chromosomal translocations that truncate the C-terminal region of TLS may prevent this interaction. Thus TLS translocations may alter RNA processing and play a role in malignant transformation.
Optimized Design of Distributed Power Systems for High Efficiency, High Power Density and Low EMI Noise

This paper first discussed the EMI issues and solutions for boost PFC converters. The parasitic cancellation technique is then introduced to improve EMI filter's high frequency performance. A two-stage PFC topology is introduced to improve the power density of PFC. LLC topology is recommended for front-end DC/DC converters due to it high efficiency. For onboard DC/DC converters, one-stage approach is first proposed. A self-driven full-bridge resonant circuit and a current tripler are introduced to improve the current density and switching frequencies. The paper also discussed the two-stage approach and intermediate bus architecture
Explaining the Variation in Empirical Estimates of Tax Elasticities of Foreign Direct Investment

This study aims to explain the variation in empirical estimates in the literature on the elasticity of foreign direct investment with respect to company tax levels. To that end, we extend the meta analysis of De Mooij and Ederveen (2003) by considering an alternative classification of the literature, including new studies that have recently become available, and by paying more systematic attention to various control variables in primary studies. We find that the type of capital data and tax data exert a systematic impact on reported elasticities. Also controlling for openness and agglomeration tendencies appears to significantly affect the elasticity values.
[The situation of children neglect between left-behind children and living-with-parents children in rural areas of two western provinces of China].

OBJECTIVE To understand the children neglect situation of left-behind children (children who do not grow up with their parents) and non-left-behind children in China's western rural and its influencing factors. METHODS Scales and evaluation methods in the "Chinese rural child neglected evaluation model" were used in this study. The investigation was conducted by using multistage stratified cluster sampling method; three countries were sampled randomly in Shanxi and Chongqing in November 2010, respectively. And, in every county, children from rural area aged from zero to six were randomly selected, with neglect rate and degree to describe their neglected status, using logistic regression analysis to analyze factors affecting the neglect rate. RESULTS Among 1568 subjects (859 left-behind and 709 living-with-parents), the total neglect rates were 29.78% (467/1568), and the total neglect degrees were 48.51 ± 6.49; the neglect rates for left-behind ones and living-with parents ones were 34.34% (295/859) and 24.26% (172/709) (P < 0.05); the neglect degree were 49.59 ± 6.54 and 47.19 ± 6.18 (P < 0.05). The neglect degree among left-behind ones and living-with parents ones between 0 to 2 years old were 48.59 ± 6.33 and 45.78 ± 5.94 (P < 0.05); in 3 to 6 years old group, which were 50.43 ± 6.60 and 48.25 ± 6.16(P < 0.05). The degrees in boy's group of these two kinds of children were 49.83 ± 6.67, 47.36 ± 6.28(P < 0.05) and girl's were 49.32 ± 6.38, 47.01 ± 6.08 (P < 0.05). On the other side, the neglect rate of left-behind and non-left-behind children between 0 to 2 years old were 39.33% (153/389) and 18.54% (56/302) (P < 0.05). The rates of boy's group were 34.91% (162/464) and 25.13% (94/374) (P < 0.05), and girl's were 33.67% (133/395) and 23.28% (78/335) (P < 0.05). Results showed that if the left-behind child's father was with lower education background, and the child and his mother feel stranger to each other, which led to the conclusion there was more chance for them to be neglected (OR values were 1.29 and 1.55, P < 0.05). If the non-neglected child's father was farmer or migrant worker and the relationship between the mother and father was poor, then there was more chance for them to be neglected (OR values were 0.85 and 1.92, P < 0.05). CONCLUSION The situation of children neglect in the western rural regions in China is serious. Both the neglect rate and degree among left-behind children are higher than those living-with-parents children. The influencing factors of neglect rate are different in the two groups.
Optimal sizing and cost of a Microgrid based in PV, WIND and BESS for a School of Engineering

Fast depleting reserves of fossil fuels, increasing the cost of fuel for conventional power generation, the use of renewable sources of power generation has become an inevitable reality. The paper makes a feasibility assessment of standalone hybrid renewable energy system based on (PV/Wind/Battery) as a possible source to electrification for an education institution namely Mohammadia School of Engineers in Rabat, Morocco, (33°59′ 58″ N, 6°51′ 7″ W). The objective of the study is to optimize the size and the cost of the microgrid system at the selected site to meet the electrical demand of a load. The modeling, optimization and analysis of the hybrid system are carried out using MATLAB software. A comparative algorithm study is carried out with Invasive Weed Optimization (IWO) and the hybrid IWO-PSO algorithm. The microgrid system is evaluated on the basis of Net Present Cost (NPC) and Levelised Cost of Energy (LCOE). Simulation results show that the Hybrid IWO-PSO have more promising results than the other algorithm.
Silver nanoparticle toxicity in the embryonic zebrafish is governed by particle dispersion and ionic environment

The mechanism of action of silver nanoparticles (AgNPs) is unclear due to the particles’ strong tendency to agglomerate. Preventing agglomeration could offer precise control of the physicochemical properties that drive biological response to AgNPs. In an attempt to control agglomeration, we exposed zebrafish embryos to AgNPs of 20 or 110 nm core size, and polypyrrolidone (PVP) or citrate surface coatings in media of varying ionic strength. AgNPs remained unagglomerated in 62.5 μM CaCl2 (CaCl2) and ultrapure water (UP), but not in standard zebrafish embryo medium (EM). Zebrafish embryos developed normally in the low ionic strength environments of CaCl2 and UP. Exposure of embryos to AgNPs suspended in UP and CaCl2 resulted in higher toxicity than suspensions in EM. 20 nm AgNPs were more toxic than 110 nm AgNPs, and the PVP coating was more toxic than the citrate coating at the same particle core size. The silver tissue burden correlated well with observed toxicity but only for those exposures where the AgNPs remained unagglomerated. Our results demonstrate that size- and surface coating-dependent toxicity is a result of AgNPs remaining unagglomerated, and thus a critical-design consideration for experiments to offer meaningful evaluations of AgNP toxicity.
Analysis and design of coupled inductor for interleaved multiphase three-level DC-DC converters

The coupled inductor can reduce the large inductor current ripple in the interleaved multiphase three-level converter, while keeping the benefits of interleaving. The design of the coupled inductor is different from conventional two-level converters. This paper analyzes the flux in the coupled inductors of interleaved multiphase three-level DC-DC converters. Based on the flux analysis, a design procedure is introduced. A new structure of the coupled inductor is also proposed which can significantly reduce the volume and is easy to fabricate.