Miracle-dz/UniFAR-FTUs huggingface dataset:
Estudios de laboratorio para el control de Ceratitis capitata (Wiedmann)(Diptera: Tephritidae)(Mosca del Mediterráneo) con Beauveria bassiana

Laboratory studies to control Ceratitis capitata (Wiedmann) (Diptera: Tephritidae) (mediterranean fly) with Beauveria bassiana. With the objective of selecting promissory isolates of Beauveria bassiana for the control of Ceratitis capitata, several bioassays were carried out, in laboratory conditions, using the adult as the vulnerable stage for the parasitic infection with the entomopathogenic fungus. For the isolate screening, a concentration of 5x10 9 VC.m -2 of the fungus was used. The LC 90 , media survival rate (MS), and insecticides compatibility were evaluated; besides, fungus production assays were done. Insecticides Mercaptotion 100% CE (Lupara®), and Dimetoato 40% CE (Sistemico Icona) were evaluated for compatibility with the selected isolates. This was done by quantification of the conidia’s viability in Petri dishes after treatments with 50, 100 and 200% of insecticides commercial doses. From 7 isolates evaluated (mortality 97-14%), Bb 26, Bb 259, Bb 132, and Bb 238 were selected. CL 90 values ranged from 1x10 9 to 3,8x10 11 VC.m -2 (Bb 238 and Bb 26, respectively). The MS of the isolates ranged between 7-12 days (Bb 238 y Bb 26, respectively). The highest yields in production were 7x10 9 CFU. mg -1 and 20,7 g.conidia. kg -1 of rice (Bb 26). All isolates were compatible to Mercaptotion. Bb 132 was the most compatible isolate to Dimetoato. In conclusion, laboratory assays allowed the selection of potencial B. bassiana isolates to control C. capitata.
Evaluation of biocontrol agents for grapevine pruning wound protection against trunk pathogen infection.

Trunk diseases of grapevine are caused by numerous pathogens, including Eutypa lata , Phaeomoniella chlamydospora , and species of Botryosphaeriaceae (incl. Botryosphaeria and aggregate genera), Phomopsis and Phaeoacremonium . Since infections occur mainly through pruning wounds, that have been shown by previous research to stay susceptible for up to 16 weeks after pruning, long-term pruning wound protection is required for prevention of infection. This study evaluated several biocontrol agents against a range of trunk disease pathogens in dual plate laboratory trials to determine macroscopic and microscopic interactions. The biocontrol agents had a substantial effect on all the pathogens, with a wide range of macroscopic and microscopic interactions observed. The best performing biocontrol agents were tested in two field trials. Fresh pruning wounds were treated with benomyl, Trichoderma products (Biotricho®, Vinevax® and ECO 77®) and isolates (USPP-T1 and -T2, identified as T. atroviride ) and Bacillus subtilis . Seven days after treatment the pruning wounds were inoculated by spraying with spore suspensions of Neofusicoccum australe , N. parvum , Diplodia seriata , Lasiodiplodia theobromae , Eutypa lata , Phaeomoniella chlamydospora or Phomopsis viticola . Eight months after inoculation, the treatments were evaluated by isolation onto potato dextrose agar. The efficacy of the biocontrol agents was in most cases similar or superior to that observed for benomyl. Isolate USPP-T1, in particular, was very effective, reducing incidence of Ph. viticola , E. lata , Pa. chlamydospora , N. australe , N. parvum , D. seriata and L. theobromae by 69, 76, 77, 78, 80, 85 and 92%, respectively. This is the first report of biological protection of grapevine pruning wounds against this group of grapevine trunk disease pathogens.
Aktivität Ubiquitin-konjugierender Enzyme an den RING-Ligasen des ERAD-Systems

8 1 Einleitung . 9 1.1 Proteinhomöostase . 9 1.2 Wege des Proteinabbaus . 9 1.3 Modifikation von Proteinen mit Ubiquitin . 10 1.4 Monooder Polyubiquitinierung . 13 1.5 E4-Enzyme . 14 1.6 Deubiquitinierende Enzyme . 14 1.7 Funktionen verschiedener Polyubiquitinierungen . 15 1.8 Ubiquitin-bindende Domänen . 15 1.9 Ubiquitin-ähnliche Domänen . 17 1.10 Endoplasmatisches Retikulum-assoziierte Proteindegradation (ERAD) . 17 1.11 RING-Ligasen in ERAD . 17 1.12 In ERAD involvierte Ubiquitin-konjugierende Enzyme. 19 1.13 Transfer der ubiquitinierten Proteine zum 26S Proteasom . 21 1.14 ERAD-Substrate . 22 1.15 Erkennung fehlerhaft gefalteter Proteine im ER . 23 1.16 UPR . 24 1.17 Protein-Dislokation . 25 1.18 Zielsetzung dieser Arbeit . 26 2 Ergebnisse . 27 2.1 In vitro Ubiquitinierung . 27 2.2 Cue1 verstärkt die Ubc7-abhängige Synthese von Ubiquitinketten in Interaktion mit den Ubiquitinligasen . 29
Does plant morphology influence fish fauna associated with seagrass meadows

Three distinct seagrass habitats were sampled to determine whether fish assemblages differed between meadows comprising of different seagrass species with different morphological characteristics and whether plant morphology influences species assemblages. Three scagrass habitats consisting of Posidonia sinuosa, Posidonia coriacea and meadows of a mixture of P. coriacea and Heterozostera tasmanica in the Success Bank region, off the coast of Fremantle, Western Australia were selected. For each habitat, sampling was carried out using a lm wide beam trawl over a distance of 50m at six replicated locations, on three occasions between June and September 2002. Seagrass samples were collected at each location. On each sampling, occasion a 0.025m quadrat was used to collect data on leaf area index (LAI), seagrass density and biomass, leaf width and length, and epiphytic algal biomass. MDS ordinations and ANOSIM showed that P. sinuosa habitats contain a significantly different composition of fish species to P. coriacea habitats (with or without H. tasmanica). Species composition was similar in P. coriacea on its own or mixed with H. tasmanica. SIMPER showed that the; differences between P. sinuosa and the P. coriacea habitats reflected the gr~ater abundances of Stigmatopora argus, Siphonognathus radiatus and Scobinichthys granulatus, whereas Stigmatopora nigra was restricted to the P. coriacea habitats only. ANOVA demonstrated that total densities and biomass of fish and species richness were greatest in P. simwsa, while little difference occurred for those variables between the twn P. coriacea habitats. ANOVA indicated that densities of S. argus and Siphamia cephalotes did not differ between habitats, while densities of S. radiatus and S. nigra differed between P. sinuosa and the P. coriacea habitats. Densities of S. granulatus differed only between P. simwsa and P. coriat:ea with H. tasmanica. Seagrass leaf density, leaf area index (LAI), leaf width, dty seagr(lsS biomass and dry epiphytic biomass differed significantly among the three habitats, where as leaf length did not differ between these habitats. Regression analysis indicated that leaf
Generalized OFDM-IM With Noncoherent Detection

This paper generalizes the conventional noncoherent OFDM-IM systems by allowing selection of different numbers of active subcarriers for message representation. We then present the maximum-likelihood (ML) detection rule for frequency-selective block fading channels. The performance of the ML receiver is analyzed in terms of pairwise-error probability as well as diversity order. Asymptotic analysis of the proposed system reveals the role of partial Hamming distance in designing diversity-exploiting activation patterns. Practical issues including low-complexity receiver, and code (activation pattern) design techniques are discussed. Furthermore, the effect of inter-carrier interference in fast fading scenarios, and generalization of the technique to multiple-antenna scenarios are studied. Finally, we present simulation results to illustrate performance benefits of the generalized OFDM-IM systems over the conventional noncoherent OFDM-IM systems.
Phylogeography of a pearl oyster (Pinctada maxima) across the Indo-Australian Archipelago: evidence of strong regional structure and population expansions but no phylogenetic breaks

This study investigates the genetic structure and phylogeography of a broadcast spawning bivalve mollusc, Pinctada maxima, throughout the Indo-West Pacific and northern Australia. DNA sequence variation of the mitochondrial cytochrome oxidase subunit I (COI) gene was analysed in 367 individuals sampled from nine populations across the Indo-West Pacific. Hierarchical AMOVA indicated strong genetic structuring amongst populations (ΦST = 0.372, P < 0.001); however, sequence divergence between the 47 haplotypes detected was low (maximum 1.8% difference) and no deep phylogenetic divergence was observed. Results suggest the presence of genetic barriers isolating populations of the South China Sea and central Indonesian regions, which, in turn, show patterns of historical separation from northern Australian regions. In P. maxima, historical vicariance during Pleistocene low sea levels is likely to have restricted planktonic larval transport, causing genetic differentiation amongst populations. However, low genetic differentiation is observed where strong ocean currents are present and is most likely due to contemporary larval transport along these pathways. Geographical association with haplotype distributions may indicate signs of early lineage sorting arising from historical population separations, yet an absence of divergent phylogenetic clades related to geography could be the consequence of periodic pulses of high genetic exchange. We compare our results with previous microsatellite DNA analysis of these P. maxima populations, and discuss implications for future conservation management of this species.
Microbial metabolism of dimethyl sulphide and related compounds

Thiobacillus MSI, a recently isolated obligately chemolithoautotrophic Thiobacillus species, which was reported to be able to utilize dimethyl sulphide as an additional carbon and/or ,energy source while growing in a thiosulphate-limited chemostat, was checked for its dimethyl sulphide oxidizing potential. Unfortu­ nately at present this organism is only available in . mixed culture. Our attempts to reis.olate the original Thiobacillus MSI from this culture were unsuccessful. Neither an obligately autotrophic Thiobacillus-type organism isolated from it nor the mixed culture itself showed any dimethyl sulphide utilizing capacity. Both cultures seemed to be insensitive to the dimethyl sulphide present in the medium.
La experimentación educativa, social y técnica en los medialabs universitarios

El presente estudio explora las actividades, los proyectos y las líneas de acción de veinticuatro medialabs de instituciones de educación superior elegidos a partir de un sistema no probabilístico. Sobre la muestra se aplica un análisis de contenido cualitativo para mapear las características de los laboratorios. Siguiendo un proceso inductivo de categorización, el estudio identifica como ejes de trabajo destacados iniciativas que materializan los principios de la inteligencia colectiva, impulsan la transformación de los sistemas de enseñanza y aprendizaje, y promueven el uso del lenguaje audiovisual y multimedia dentro y fuera del aula. Los resultados subrayan patrones de enseñanza que estrechan los vínculos de las instituciones con sus entornos sociales. Las iniciativas encontradas muestran de qué manera los medialabs facilitan nuevas formas de alcanzar el conocimiento en áreas tan variadas como el arte, el diseño, el periodismo o la ingeniería a través del uso de la tecnología.
A Mathematical Model of the Cell Differentiation in Multicellular Eukaryotes

The cell differentiation in multicellular eukaryotes is one of the most curious phenomena. The recent gene and genome sequencing reveals that most of differentiated cells in a multicellular eukaryote carry a common genome and that such a genome contains the expanded repertoire of genes of proteins associated with the cell-cell adhesion, intercellular and intracellular signal transduction and transcriptional regulation. The cell differentiation occurs in the assembly consisting of a large number of cells after the cell proliferation, and this process is regarded as a stochastic process. Its formulation starts with the master equation in the present paper. The cell differentiation is reproduced in the equation of the most probable path derived from the master equation, when the short-range and long-range interactions between the cells as well as the transition probability between the proliferation and differentiation modes are considered. Moreover, the equation of the most probable path explains the experimental results such as the “memory”, tissue culture and the preparation of induced pluripotent stem (iPS) cells in embryology, if the long-range interaction is considered to be the regulation of gene transcription under the influence of intracellular signal transduction from the receptor accepting the ligand secreted by other types of cells and the short-range interaction is considered to stabilize the intracellular signal transduction by the contact between the same type of cells. The “organizer” found in the initial development of embryo is also explained as the cells that preferentially express the specific gene of a ligand to rouse the long-range interaction. In conclusion, the present study proposes that the complicated intercellular and intracellular signal transduction causing the cell differentiation is ascribed to the long-range interaction between distinctive types of cells and the short-range interaction between the same type of cells.
‘Fair Game?’ (A Participatory Game Based on Bourdieu’s Theoretical Concept About Agency in Life)

‘Fair Game? ' is a participatory game that was designed to allow its players to comprehend the possibility of life choices and cultural capital throughout their lifespan. The game's objective is to engage the players with multiple questions about life choices and involve in a dialogue. 'Fair Game? ' is likely a suitable platform to mediate a dialogue through participation, utilizing a concept that individuals will be able to establish a connection to the rules of the game from their acquired knowledge of their own specific cultural setting, as well as forge each of their own agency in the game that is basically imitates and illustrates the life itself. The methodology used in generating this game is qualitative data through journal and article reading and testing on several game platforms. After the theory and concept are formulated, the game itself is formed to adapt the surroundings, especially the people who participates in it. The game's visualization imitates a traditional game like hopscotch, also combined with snakes and ladders to illustrates about agency. By the end of the game, the participants reflect on how the dice give them a sense of agency but also limits it, which proves Bourdieu’s theoretical concept about agency and decision in life not just depends on the how human develop their life with their own ability but also their capability which governed by cultural capital of every player.
Hybrid strategy for traffic light detection by combining classical and self‐learning detectors

Detection of the traffic light is a key function of the automatic driving system for urban traffic. Considering the characteristics of classical and self-learning algorithms, a fusion logic is proposed to make up the shortcoming of learning algorithms by combining the known knowledge with the learning features to detect the red and yellow–green traffic light without turn indicator. The relationship of detection performance among different detectors is established analytically. Then the improvement of detection performance by fusion is analysed theoretically and optimised numerically. According to the analysis results, the hybrid detector is designed by using the colour information in hue-saturation-intensity to extract the candidate region, the hog feature to identify the shape information of traffic light classified by a support vector machine, and a comparatively simple convolutional neural network (CNN) with the classical AlexNet structure to act as the self-learned detector. The effectiveness of the hybrid method is validated by several comparative tests with single CNN detectors and other fusion methods on the training dataset, and the extensibility to new application conditions is evaluated by vehicle tests.
Transcript-Specific Cytoplasmic Degradation of YRA1 Pre-mRNA Mediated by the Yeast EDC3 Protein: A Dissertation

mRNA degradation is a fundamental process that controls both the level and the fidelity of gene expression. Using a combination of bioinformatic, genomic, genetic, and molecular biology approaches, we have shown that Edc3p, a yeast mRNA decay factor, controls the stability of the intron-containing YRA1 pre-mRNA. We found that Edc3p-mediated degradation of YRA1 pre-mRNA: 1) is a component of a negative feedback loop involved in the autoregulation of YRA1 , 2) takes place in the cytoplasm, 3) is independent of translation, 4) occurs through a deadenylation-independent decapping and 5΄ to 3΄ exonucleotic decay mechanism, and 5) is controlled by specific cis -acting elements and trans -regulatory factors. Cis -regulation of YRA1 pre-mRNA degradation is complicated and precise. Sequences in exon1 inhibit YRA1 pre-mRNA splicing and/or promote pre-mRNA export in a size-dependent but sequence-independent manner. Sequences in the intron dictate the substrate specificity for Edc3p-mediated decay. Five structurally different but functionally interdependent modules were identified in the YRA1 intron. Two modules, designated Edc3p-responsive elements (EREs), are required for triggering an Edc3p-response. Three other modules, designated translational repression elements (TREs), are required for repressing translation of YRA1 pre-mRNA. TREs enhance the efficiency of the response of the EREs to Edc3p by inhibiting translation-dependent nonsense-mediated mRNA decay (NMD). Trans -regulation of YRA1 pre-mRNA is governed by Yra1p, which inhibits YRA1 pre-mRNA splicing and commits the pre-mRNA to nuclear export, and the RNP export factors, Mex67p and Crm1p, which jointly promote YRA1 pre-mRNA export. Mex67p also appears to interact with sequences in the YRA1 intron to promote translational repression and to enhance the Edc3p response of YRA1 pre-mRNA. These results illustrate how common steps in the nuclear processing, export, and degradation of a transcript can be uniquely combined to control the expression of a specific gene and suggest that Edc3p-mediated decay may have additional regulatory functions in eukaryotic cells.
GPU-Accelerated Vision for Robots: Improving System Throughput Using OpenCV and CUDA

OpenCV is an open source computer vision and machine learning library for C/C++/Python available for Windows, Linux, macOS, and Android platforms. It contains low-level image processing functions as well as high-level algorithms such as object identification, face recognition, and action classification in videos. OpenCV has become very popular, with more than 47,000 people in its user community and 18 million downloads (see https://opencv.org/about/). Under a Berkeley Software Distribution (BSD) license, it can be used for both academic and commercial applications.
PATHOLOGICAL CHANGES IN THE LIVER OF DIPODILLUS DASYURUS DASYURUS INFECTED WITH SCHISTOSOMA MANSONI

The present study was undertaken to investigate the pathological changes in the liver of the dipodil Dipodillus dasyurus dasyurus of the order Rodentia, Family Cricetidae due to Schistosoma mansoni infection. Animals were individually exposed to 90 - 100 S. mansoni cercariae of Egyptian strain by the tail immersion technique. The pathological changes at autopsy 10 weeks post infection were evaluated by determination of the liver and spleen relative weights, hepatic granuloma volume, and biochemical assay of hepatic dysfunction. For comparison, Mus Musculus, the laboratory permissive host, was used. The current results showed a significant increase in the relative liver and spleen weights of infected dipodils as compared with normal ones. Typical granulomatous reaction was observed in infected rodents. Granulomas from the dipodil (50.4 Â± 23.3 Âµm3) were significantly smaller than those of the mouse (111.3 Â± 69.1 Âµm3). Furthermore, S. mansoni infection caused marked hepatic dysfunction, as manifested by the decrease in the enzymatic activity of liver cholinesterase, arginase, alanine aminotransferase, and aspartate aminotransferase, in addition to the decrease in the liver contents of potassium and total protein. The results also demonstrated a decrease in the levels of serum albumin and urea. Nonetheless, the liver contents of sodium and total lipids were elevated in response to the infection. In conclusion, D. d. dasyurus, compared with the laboratory definitive host Mus Musculus, tolerates well S. mansoni infection and is a potential maintenance host of S. mansoni.
Roles of nicotinic acetylcholine receptor β subunits in function of human α4‐containing nicotinic receptors

Naturally expressed nicotinic acetylcholine receptors (nAChR) containing α4 subunits (α4*‐nAChR) in combination with β2 subunits (α4β2‐nAChR) are among the most abundant, high‐affinity nicotine binding sites in the mammalian brain. β4 subunits are also richly expressed and colocalize with α4 subunits in several brain regions implicated in behavioural responses to nicotine and nicotine dependence. Thus, α4β4‐nAChR also may exist and play important functional roles. In this study, properties were determined of human α4β2‐ and α4β4‐nAChR heterologously expressed de novo in human SH‐EP1 epithelial cells. Whole‐cell currents mediated via human α4β4‐nAChR have ∼4‐fold higher amplitude than those mediated via human α4β2‐nAChR and exhibit much slower acute desensitization and functional rundown. Nicotinic agonists induce peak whole‐cell current responses typically with higher functional potency at α4β4‐nAChR than at α4β2‐nAChR. Cytisine and lobeline serve as full agonists at α4β4‐nAChR but are only partial agonists at α4β2‐nAChR. However, nicotinic antagonists, except hexamethonium, have comparable affinities for functional α4β2‐ and α4β4‐nAChR. Whole‐cell current responses show stronger inward rectification for α4β2‐nAChR than for α4β4‐nAChR at a positive holding potential. Collectively, these findings demonstrate that human nAChR β2 or β4 subunits can combine with α4 subunits to generate two forms of α4*‐nAChR with distinctive physiological and pharmacological features. Diversity in α4*‐nAChR is of potential relevance to nervous system function, disease, and nicotine dependence.