Miracle-dz/UniFAR-FTUs huggingface dataset:
Process development for cell aggregate arrays encapsulated in a synthetic hydrogel using negative dielectrophoresis

Spatial patterning of cells is of great importance in tissue engineering and biotechnology, enabling, for example the creation of bottom‐up histoarchitectures of heterogeneous cells, or cell aggregates for in vitro high‐throughput toxicological and therapeutic studies within 3D microenvironments. In this paper, a single‐step process for creating peelable and resilient hydrogels, encapsulating arrays of biological cell aggregates formed by negative DEP has been devised. The dielectrophoretic trapping within low‐energy regions of the DEP‐dot array reduces cell exposure to high field stresses while creating distinguishable, evenly spaced arrays of aggregates. In addition to using an optimal combination of PEG diacrylate pre‐polymer solution concentration and a novel UV exposure mechanism, total processing time was reduced. With a continuous phase medium of PEG diacrylate at 15% v/v concentration, effective dielectrophoretic cell patterned arrays and photo‐polymerisation of the mixture was achieved within a 4 min period. This unique single‐step process was achieved using a 30 s UV exposure time frame within a dedicated, wide exposure area DEP light box system. To demonstrate the developed process, aggregates of yeast, human leukemic (K562) and HeLa cells were immobilised in an array format within the hydrogel. Relative cell viability for both cells within the hydrogels, after maintaining them in appropriate iso‐osmotic media, over a week period was greater than 90%.
ighting in the home cage : Agonistic encounters and effects on eurobiological markers within the social decision-making network f house mice ( Mus musculus )

Inbred strains of mice, such as C57Bl/6, have become preferred animal models for neurobehavioral studies. A main goal in creating inbred lines is to reduce the effects of individual genetic variation on observed phenotypes. Most studies use only males, and there is increasing evidence that agonistic interactions within the home cage may produce systematic variability in behavior and brain function. Previous studies have demonstrated that the outcomes of aggressive interactions have powerful effects on the brain and behavior, but less is known about whether aggressive interactions within the home cage have similar effects. We assessed group-housed laboratory mice C57Bl/6 for competitive ability and then tested the extent high competitive ability (CA) or low CA was related to gene and protein expression within related ndrogen receptor strogen receptor pathways. We focused on a broad social behavior network, including the nucleus accumbens (NAc) and bed nucleus of the stria terminalis (BNST). High CA mice had significantly more corticotropin releasing hormone receptor 2 (CRHR2) and estrogen receptor alpha (ESR1) mRNA in the BNST. Our data suggest a simple test of CA could yield valuable information that could be used to reduce error variance and increase power in neurobiological studies using mice. © 2014 Elsevier Ireland Ltd. All rights reserved. Abbreviations: CA, competitive ability; NA, nucleus accumbens; BNST, ed nucleus of the stria terminalis; AR, androgen receptor; CRHR2, corticropin releasing hormone receptor 2; ESR1, estrogen receptor alpha; TBST-T, riton-X in tris-buffered saline; PMSF, phenylmethylsulfonyl fluoride; HEPES4, -(2ydroxyethyl)-1-piperazineethanesulfonic acid; ETDA, ethylenediaminetetraacetic cid. ∗ Corresponding author at: University of California, Davis, Center for Neurocience, 1544 Newton Court, Davis, CA 95616, USA. Tel. : +1 530 754 8047; ax: +1 530 757 8827. E-mail addresses: ggreenberg@ucdavis.edu, giangreenberg@hotmail.com G.D. Greenberg). ttp://dx.doi. org/10.1016/j.neulet. 2014.02. 051 304-3940/© 2014 Elsevier Ireland Ltd. All rights reserved.
Intra voxel analysis in MRI

A new application of Compressive Sensing (CS) in Magnetic Resonance Imaging (MRI) field is presented. In particular, first results of the Intra Voxel Analysis (IVA) technique are reported. The idea is to exploit CS peculiarities in order to distinguish different contributions inside the same resolution cell, instead of reconstructing images from not fully sampled k-space acquisition. Applied to MRI field, this means the possibility of estimating the presence of different tissues inside the same voxel, i.e. in one pixel of the obtained image. In other words, the method is the first attempt, as far as we know, of achieving Spectroscopy-like results starting from each pixel of MR images. In particular, tissues are distinguished each others by evaluating their spin-spin relaxation times. Within this manuscript, first results on clinical dataset, in particular a phantom made by aqueous solution and oil and an occipital brain lesion corresponding to a metastatic breast cancer nodule, are reported. Considering the phantom dataset, in particular focusing on the slice where the separation between water and oil occurs, the methodology is able to distinguish the two components with different spin-spin relaxation times. With respect to clinical dataset,focusing on a voxel of the lesion area, the approach is able to detect the presence of two tissues, namely the healthy and the cancer related ones, while in other location outside the lesion only the healthy tissue is detected. Of course, these are the first results of the proposed methodology, further studies on different types of clinical datasets are required in order to widely validate the approach. Although few datasets have been considered, results seem both interesting and promising.
Factors of learner-instructor interaction which predict perceived learning outcomes in online learning environment

Interaction in the online learning environment has been regarded as one of the most critical elements that affect learning outcomes. This study examined what factors in learner–instructor interaction can predict the learner's outcomes in the online learning environment. Learners in K Online University participated by answering the survey, and data from 654 respondents were analysed for this study. Results showed that factors related to instructional interaction predicted perceived learning achievement and satisfaction better than factors related to social interaction. However, it was revealed that social interaction such as social intimacy could negatively affect perceived learning achievement and satisfaction. This study has value because it found factors under learner–instructor interaction which predict perceived learning achievement and satisfaction with empirical evidence.
A tabular steganography scheme for graphical password authentication

Authentication, authorization and auditing are the most important issues of security on data communication. In particular, authentication is the life of every individual essential closest friend. The user authentication security is dependent on the strength of user password. A secure password is usually random, strange, very long and difficult to remember. For most users, remember these irregular passwords are very difficult. To easily remember and security are two sides of one coin. In this paper, we propose a new graphical password authentication protocol to solve this problem. Graphical password authentication technology is the use of click on the image to replace input some characters. The graphical user interface can help user easy to create and remember their secure passwords. However, in the graphical password system based on images can provide an alternative password, but too many images will be a large database to store issue. All the information can be steganography to achieve our scheme to solve the problem of database storage. Furthermore, tabular steganography technique can achieve our scheme to solve the information eavesdropping problem during data transmission. Our modified graphical password system can help user easily and friendly to memorize their password and without loss of any security of authentication. User‟s chosen input will be hidden into image using steganography technology, and will be transferred to server security without any hacker problem. And then, our authentication server only needs to store only a secret key for decryption instead of large password database.
Better Interprofessional Teamwork, Higher Level of Organized Care, and Lower Risk of Burnout in Acute Health Care Teams Using Care Pathways: A Cluster Randomized Controlled Trial

Background:Effective interprofessional teamwork is an essential component for the delivery of high-quality patient care in an increasingly complex medical environment. The objective is to evaluate whether the implementation of care pathways (CPs) improves teamwork in an acute hospital setting. Design and Measures:A posttest-only cluster randomized controlled trial was performed in Belgian acute hospitals. Teams caring for patients hospitalized with a proximal femur fracture and those hospitalized with an exacerbation of chronic obstructive pulmonary disease, were randomized into intervention and control groups. The intervention group implemented a CP. The control group provided usual care. A set of team input, process, and output indicators were used as effect measures. To analyze the results, we performed multilevel statistical analysis. Results:Thirty teams and a total of 581 individual team members participated. The intervention teams scored significantly better in conflict management [&bgr;=0.30 (0.11); 95% confidence interval (CI), 0.08 to 0.53]; team climate for innovation [&bgr;=0.29 (0.10); 95% CI, 0.09 to 0.49]; and level of organized care [&bgr;=5.56 (2.05); 95% CI, 1.35 to 9.76]. They also showed lower risk of burnout as they scored significantly lower in emotional exhaustion [&bgr;=−0.57 (0.21); 95% CI, −1.00 to −0.14] and higher in the level of competence (&bgr;=0.39; 95% CI, 0.15 to 0.64). No significant effect was found on relational coordination. Conclusions:CPs are effective interventions for improving teamwork, increasing the organizational level of care processes, and decreasing risk of burnout for health care teams in an acute hospital setting. Through this, high-performance teams can be built.
Pharmacological properties and SAR of new 1,4‐disubstituted piperazine derivatives with hypnotic‐sedative activity

Preparation, pharmacological properties and structure‐activity relationships of new pyrimidylpiperazine derivatives, exhibiting sedative and hypnotic activity in mice, are reported. The hypnotic activity of the compounds was comparable with that of zopiclone (the known hypnotic‐sedative agent), their interaction with ethanol, however, being much lower. The obtained results suggested that zopiclone and pyrimidylpiperazines 2, 4 and 5 exerted their pharmacological activity through a different mechanism — zopiclone through the interaction with benzodiazepine receptors and compounds 2, 4 and 5 through an unidentified molecular target. The pharmacological properties of compound 3 could be the result of a mixed mechanism of action, combining the properties of zopiclone and those of compounds 2, 4 and 5. A common feature of zopiclone and compounds 2 and 3 was that, after their systemic administration, independently of mechanism of action, together with the hypnotic effect a reduction of the 5‐HT turnover in the mouse brain was observed. Minimum structural requirements for the hypnotic activity were formulated. Structural considerations have shown that removing the α‐carbonyl group did not influence the drug's ability to inhibit the locomotor activity. However, it did influence its ability to disturb motor coordination or abolish the righting reflex within non‐lethal doses.
Structure and function of the bacterial mechanosensitive channel of large conductance

Mechanosensation in bacteria involves transducing membrane stress into an electrochemical response. In Escherichia coli and other bacteria, this function is carried out by a number of proteins including MscL, the mechanosensitive channel of large conductance. MscL is the best characterized of all mechanosensitive channels. It has been the subject of numerous structural and functional investigations. The explosion in experimental data on MscL recently culminated in the solution of the three‐dimensional structure of the MscL homologue from Mycobacterium tuberculosis. In this review, much of these data are united and interpreted in terms of the newly published M. tuberculosis MscL crystal structure.
PROCESS OPTIMIZATION USING ANT COLONY CLUSTERING TECHNIQUE

Ant Colony Optimization is a population based meta-heuristic that can be used to find approximate solutions to optimization problems. The clustering analysis divides data into groups (clusters) such that similar data objects belong to the same cluster and dissimilar data objects to different clusters. The clustering based on ant’s behavior is used for process optimization. This paper focuses on water treatment process in which the output chemical needs to be at optimum level to get the desired turbidity range in the outlet water. Initially k-means clustering is used to cluster the input chemical values and then refining of these clusters is done by ant based clustering. A single ant can move the data among clusters depending on picking up and dropping probabilities. The performance measures such as Performance Index (PI) and Standard Deviation (%SD) are used to validate the results from k-means clustering and ant based clustering.
Proton MR spectroscopy of the basal ganglia in healthy children and children with AIDS.

PURPOSE To evaluate proton magnetic resonance (MR) spectroscopy in children with the acquired immunodeficiency syndrome (AIDS) and to establish an age-dependent spectroscopic database of the normal basal ganglia in children. MATERIALS AND METHODS Eighteen healthy children and 45 children with AIDS underwent both brain MR imaging and single-voxel MR spectroscopy with a long-echo-time point-resolved technique. A large part of the region of interest studied at MR spectroscopy included the basal ganglia. RESULTS Seven patients with progressive encephalopathy and eight with static encephalopathy had significantly lower mean N-acetyl aspartate (NAA)/creatine (Cr) ratios than age-matched control subjects (P<.02). In determining the presence of progressive encephalopathy in children with AIDS, MR spectroscopy appears to be more sensitive and specific than MR imaging and immunologic testing. Thirty patients without encephalopathy had normal NAA/Cr ratios but significantly lower choline/Cr ratios than age-matched control subjects (P<.02). CONCLUSION Proton MR spectroscopy may be a more sensitive diagnostic technique than MR imaging in childhood AIDS encephalopathy.
Impact of NO-synthase inhibition on renal hemodynamics in normotensive and hypertensive subjects

Objective To examine the acute effects of NO-synthase inhibition on renal hemodynamics in normotensive and hypertensive subjects. Methods Changes of renal plasma flow (RPF) and glomerular filtration rate (GFR) in response to intravenous infusions of NG-monomethyl-l-arginine (l-NMMA) (3 mg/kg per 30 min) were measured in 32 normotensive and in 39 essential hypertensive patients by use of clearance technique. Results l-NMMA significantly decreased RPF in normotensive and hypertensive individuals (P < 0.001), while GFR was preserved. Changes of renal hemodynamic parameters were similar in hypertensive and normotensive subjects (ΔRPF: –88 ± 89 versus –81 ± 105 ml/min, P = NS; ΔGFR 1.6 ± 8.2 versus 4.3 ± 8.9 ml/min, P = NS) Furthermore, l-NMMA increased mean arterial pressure (ΔMAP 5.3 ± 6.3 versus 6.0 ± 6.1 mmHg, P = NS) and decreased heart rate (ΔHR –5.8 ± 3.9 versus –4.1 ± 3.8 beats/min, P = NS) to a similar extent in both groups. Conclusion Basal NO synthesis of the renal vasculature is not impaired in patients with established essential hypertension.
Corpus Luteum Hemorrhage in Women with Bleeding Disorders

Bleeding into the corpus luteum following ovulation rarely has clinical significance in healthy women, but may lead to life-threatening hemorrhage in women with congenital or acquired bleeding disorders. Women who are at an increased risk for corpus luteum hemorrhage (CLH) can be divided in two categories; first, those taking anticoagulants because of a thrombotic disorder; and second, women with congenital bleeding disorders. The management and prevention of CLH is still unsettled and the literature dealing with this problem is based on case reports only. This review focuses on the pathophysiology, clinical presentation, diagnosis and treatment options of an acute bleeding event and prevention modalities of CLH in women with bleeding disorders.
Dbo/Henji Modulates Synaptic dPAK to Gate Glutamate Receptor Abundance and Postsynaptic Response

In response to environmental and physiological changes, the synapse manifests plasticity while simultaneously maintains homeostasis. Here, we analyzed mutant synapses of henji, also known as dbo, at the Drosophila neuromuscular junction (NMJ). In henji mutants, NMJ growth is defective with appearance of satellite boutons. Transmission electron microscopy analysis indicates that the synaptic membrane region is expanded. The postsynaptic density (PSD) houses glutamate receptors GluRIIA and GluRIIB, which have distinct transmission properties. In henji mutants, GluRIIA abundance is upregulated but that of GluRIIB is not. Electrophysiological results also support a GluR compositional shift towards a higher IIA/IIB ratio at henji NMJs. Strikingly, dPAK, a positive regulator for GluRIIA synaptic localization, accumulates at the henji PSD. Reducing the dpak gene dosage suppresses satellite boutons and GluRIIA accumulation at henji NMJs. In addition, dPAK associated with Henji through the Kelch repeats which is the domain essential for Henji localization and function at postsynapses. We propose that Henji acts at postsynapses to restrict both presynaptic bouton growth and postsynaptic GluRIIA abundance by modulating dPAK.
First-principles modeling of double-gate UTSOI MOSFETs

This paper presents a first-principles approach to modeling wavefunction penetration and transport in UTSOI MOSFETs that includes accurate quantum mechanics and atomic-scale structures with no empirical parameters. Applications to interface roughness and to Hf atoms near the Si-SiO<sub>2</sub>/HfO<sub>2</sub> interface in double-gate UTSOI MOSFETs with SiO<sub>2</sub> or HfO<sub>2</sub> gate dielectrics are presented
Development of a Smart Shoe for Building a Real-Time 3D Map

Article history: Accepted 11 March 2016 Available online 9 April 2016 Three dimensional (3D)mapping of environments has gained tremendous attention, from academic to industry to military, owing to the ever increasing needs of environmental modeling as well as monitoring. While many highly effective techniques have been reported thus far, a few even turned into commercial products, none has explored the use of wearable sensors to capture human foot motion, gait, and phase for 3D map construction, especially in the Architecture, Engineering, and Construction (AEC) domain. In this work, we propose a smart (andwearable) shoe, called “Smart Shoe”, which integratesmultiple laser scanners and an inertial measurement unit (IMU) to build a 3D map of environments in real time. Such a Smart Shoe can be a potential tool for floor plan surveying, construction process monitoring, planning renovations, space usage planning, managing building maintenance and other tasks in the AEC domain. Besides, this Smart Shoe could assist disabled people (blind people) to navigate and avoid obstacles in the unknown environment. In another case, the shoes may help firefighters quickly model and recognize objects in the firing, dark, and smoky buildings where traditional camera-based approaches might not be applicable. We integrate this shoe with a novel foot localization algorithm that produces a smooth and accurate pose and trajectory of human walking, which is the key enabling technique to minimize data registration errors from laser point cloud. © 2016 Elsevier B.V. All rights reserved.
A family‐based model to predict fear of recurrence for cancer survivors and their caregivers

Although fear of cancer recurrence is a great concern among survivors and their families, few studies have examined predictors of fear of recurrence. The purpose of this study was to identify factors associated with fear of recurrence in a population‐based sample (N = 246) and determine if survivors and family caregivers influenced one another's fear of recurrence. A family framework guided the study and analyses included multilevel modeling using the Actor‐Partner Interdependence Model. Results indicated that survivors and family caregivers influenced each other's fear of recurrence and that caregivers had significantly more fear of recurrence than survivors. More family stressors, less positive meaning of the illness, and age were related to elevated fear of cancer recurrence for both survivors and caregivers. Copyright © 2006 John Wiley & Sons, Ltd.
Erythromelalgic, thrombotic and haemorrhagic manifestations of thrombocythaemia.

OBJECTIVES In order to document a relationship between platelet counts and clinical complication of thrombosis or bleeding in patients with myeloproliferative thrombocythaemia, 200 consecutive published cases of thrombocythaemia were reviewed for thrombotic or haemorrhagic manifestations and haematological findings at the time of the thrombohaemorrhagic complication. METHODS Cases selected from the literature included all those complying with the diagnosis of thrombocythaemia as mandated by the Polycythemia Vera Study Group. One hundred consecutive cases of thrombocythaemia complicated by bleedings and one hundred consecutive cases of thrombocythaemia complicated by thrombosis were reviewed for clinical manifestations and haematological findings. RESULTS In the reviewed cases of thrombocythaemia haemorrhagic complications (platelet count 2016 +/- 1070 x 10(9)/1) occurred at significantly (p < 0.001) higher degrees of thrombocytosis than thrombotic complications (platelet count 1110 +/- 477 x 10(9)/1). CONCLUSIONS In the reviewed cases of thrombocythaemia arterial complications, especially erythromelalgia, can already be documented at platelet counts in excess of 400 x 10(9)/1, whereas bleeding complications usually occur at significantly higher degrees of thrombocytosis (> 1000 x 10(9)/1).
Teaching Medical Students History Taking Content: A Systematic Review

Context: The medical interview is a cornerstone of clinical practice. Teaching medical students how to take a history can be broadly divided into two components: teaching the process of history taking and teaching the content of history taking. While there is a growing awareness of how history taking processes can be taught, effective history taking content teaching methods remain unclear. Objectives: To identify educational interventions targeting history taking content and how they have improved medical students’ performance. Methods: A literature search in Medline, PsycINFO, Embase and ERIC was performed independently by two authors encompassing 1980 to 2015. Only studies focusing on improving undergraduate medical student history taking content were included. Results: Six articles were included: four Randomized Controlled Trials and two Quasi-Experimental studies. All interventions were additional to traditional teaching methods. Two studies investigated the use of online video demonstrations while two other studies examined the use of computer and mannequin-based human patient simulations. One study investigated the use of a virtual clinic platform while the last study used a written structured history taking pro forma. Outcome measures included: Objective Structured Clinical Examinations (OSCE), Standardized Patient Encounters, written tests and case histories. Overall, five of the six studies showed a positive impact on medical student performance. Conclusions: Most studies in this review showed a positive impact on student performance via objective assessments. While the majority utilized electronic learning methods, there are very limited studies focusing on educational interventions targeting history taking content.