Miracle-dz/UniFAR-FTUs huggingface dataset:
Expression of Dopamine-Related Genes in Four Human Brain Regions

A better understanding of dopaminergic gene expression will inform future treatment options for many different neurologic and psychiatric conditions. Here, we utilized the National Institutes of Health’s Genotype-Tissue Expression project (GTEx) dataset to investigate genotype by expression associations in seven dopamine pathway genes (ANKK1, DBH, DRD1, DRD2, DRD3, DRD5, and SLC6A3) in and across four human brain tissues (prefrontal cortex, nucleus accumbens, substantia nigra, and hippocampus). We found that age alters expression of DRD1 in the nucleus accumbens and prefrontal cortex, DRD3 in the nucleus accumbens, and DRD5 in the hippocampus and prefrontal cortex. Sex was associated with expression of DRD5 in substantia nigra and hippocampus, and SLC6A3 in substantia nigra. We found that three linkage disequilibrium blocks of SNPs, all located in DRD2, were associated with alterations in expression across all four tissues. These demographic characteristic associations and these variants should be further investigated for use in screening, diagnosis, and future treatment of neurological and psychiatric conditions.
Pleuroperitoneal true diaphragmatic hernia of the liver in a cat

Abstract A 2-year old female domestic shorthair cat was referred with intermittent vomiting. Laboratory analyses revealed only an elevated alanine aminotransferase activity. Plain thoracic radiographs showed a right-sided transdiaphragm protrusion of a liver lobe into the thoracic cavity. On abdominal ultrasound the diaphragm and liver parenchyma also presented a bulge towards the thoracic cavity. By cranial median laparotomy herniation of the quadrate liver lobe through the right aspect of the diaphragm into the thoracic cavity was confirmed. The protruding quadrate liver lobe was covered by intact parietal peritoneum. Following relocation of the liver lobe the defect was closed. Postoperatively the intermittent vomiting stopped. The purpose of this case report was to present plain radiography and abdominal ultrasonography as effective tools in the diagnosis of this rare and usually asymptomatic true herniation case along with its operative treatment.
Extraction and Analysis of Blue Steel Roofs Information Based on CNN Using Gaofen-2 Imageries

Blue steel roof is advantageous for its low cost, durability, and ease of installation. It is generally used by industrial areas. The accurate and rapid mapping of blue steel roof is important for the preliminary assessment of inefficient industrial areas and is one of the key elements for quantifying environmental issues like urban heat islands. Here, the DeeplabV3+ semantic segmentation neural network based on GaoFen-2 images was used to analyze the quantity and spatial distribution of blue steel roofs in the Nanhai district, Foshan (including the towns of Shishan, Guicheng, Dali, and Lishui), which is the important manufacturing industry base of China. We found that: (1) the DeeplabV3+ performs well with an overall accuracy of 92%, higher than the maximum likelihood classification; (2) the distribution of blue steel roofs was not even across the whole study area, but they were evenly distributed within the town scale; and (3) strong positive correlation was observed between blue steel roofs area and industrial gross output. These results not only can be used to detect the inefficient industrial areas for regional planning but also provide fundamental data for studies of urban environmental issues.
The Effect of Height on Drop Jumps in Relation to Somatic Parameters and Landing Kinetics

The aim of this study was to assess the effect of drop height and selected somatic parameters on the landing kinetics of rebound jumps in force and power production, performed by male and female student athletes. Twenty female and forty male students with a sports background participated in the experiment (mean and standard deviation (± SD): age 20.28 ± 1.31 years, height 166.78 ± 5.29 cm, mass 62.23 ± 7.21 kg and 21.18 ± 1.29, 182.18 ± 6.43, 78.65 ± 7.09). Each participant performed three maximal jumps on two independent and synchronized force platforms (Bilateral Tensiometric Platform S2P) at each of the two assigned drop-jump heights (20-, and 40-, cm for female and 30-, and 60-, cm for the male special platform). Significant between-sex differences were observed in all variables of selected somatics, with men outperforming women. Statistically significant differences were noted in four parameters, between men and women, in both DJs from 20/40 and 30/60 cm. The height of the jump was 6 cm and 4 cm higher for men. A slightly higher statistical significance (p = 0.011) was demonstrated by the relative strength (% BW) generated by the left limb in both men and women. Only women showed a significant relationship between body mass, body height, and five parameters, dropping off of a 20 cm box. In men, only the left leg—relative maximal F (p =−0.45)—showed a relationship with body mass. There were no relationships between the above-mentioned dependencies in both groups, in jumps from a higher height: 40 cm and 60 cm. From a practical application, the DJ with lower 20/30 cm or higher 40/60 cm (women/men) respectively emphasizes either the force or power output via an increase in the velocity component of the rebound action or increased height of the DJ jump.
Expression and Purification of Recombinant GHK Tripeptides Are Able to Protect against Acute Cardiotoxicity from Exposure to Waterborne-Copper in Zebrafish

In this study, an alternative method is developed to replace chemical synthesis to produce glycyl-histidyl-lysine (GHK) tripeptides with a bacterial fermentation system. The target GHK tripeptides are cloned into expression plasmids carrying histidine-glutathione-S-transferase (GST) double tags and TEV (tobacco etch virus) cleavage sites at the N-terminus. After overexpression in Escherichia coli (E. coli) BL21 cells, the recombinant proteins are purified and recovered by high-pressure liquid chromatography (HPLC). UV-vis absorption spectroscopy was used to investigate the chemical and biological properties of the recombinant GHK tripeptides. The results demonstrated that one recombinant GHK tripeptide can bind one copper ion to form a GHK-Cu complex with high affinity, and the recombinant GHK peptide to copper ion ratio is 1:1. X-ray absorption near-edge spectroscopy (XANES) of the copper ions indicated that the oxidation state of copper in the recombinant GHK-Cu complexes here was Cu(II). All of the optical spectrum evidence suggests that the recombinant GHK tripeptide appears to possess the same biophysical and biochemical features as the GHK tripeptide isolated from human plasma. Due to the high binding affinity of GHK tripeptides to copper ions, we used zebrafish as an in vivo model to elucidate whether recombinant GHK tripeptides possess detoxification potential against the cardiotoxicity raised by waterborne Cu(II) exposure. Here, exposure to Cu(II) induced bradycardia and heartbeat irregularity in zebrafish larvae; however, the administration of GHK tripeptides could rescue those experiencing cardiotoxicity, even at the lowest concentration of 1 nM, where the GHK-Cu complex minimized CuSO4-induced cardiotoxicity effects at a GHK:Cu ratio of 1:10. On the other hand, copper and the combination with the GHK tripeptide did not significantly alter other cardiovascular parameters, including stroke volume, ejection fraction, and fractional shortening. Meanwhile, the heart rate and cardiac output were boosted after exposure with 1 nM of GHK peptides. In this study, recombinant GHK tripeptide expression was performed, along with purification and chemical property characterization, which revealed a potent cardiotoxicity protection function in vivo with zebrafish for the first time.
Validity and Reliability of the Korean Version of the Pregnancy Physical Activity Questionnaire

The purpose of this study was to translate the Pregnancy Physical Activity Questionnaire, a semi-quantitative tool that asks participants about time spent on 32 activities, into Korean and verify its validity and reliability. In total, 363 pregnant women under prenatal care at an obstetrics and gynecology hospital and a postpartum care facility in Gyeonggi-do completed the Korean version of the Pregnancy Physical Activity Questionnaire. The questionnaire’s content validity, construct validity, concurrent validity, and reliability were verified. After verifying the validity of the contents of the Pregnancy Physical Activity Questionnaire, all the questions were included in the Korean version. For construct validity, we divided the participants into primipara and multipara groups based on their delivery history. On comparison of the two groups’ physical activity based on the responses to the Pregnancy Physical Activity Questionnaire, there was a statistically significant difference in the total activity (t = −4.56, p < 0.001) and the total activity (light activity or more) (t = −5.80, p < 0.001). The correlation between the Pregnancy Physical Activity Questionnaire and the Global Physical Activity Questionnaire was tested to establish concurrent validity, and a significant correlation was found between all items except for vigorous physical activity. The Guttmann reliability coefficient by the odd-even method was 84. The Korean version of the Pregnancy Physical Activity Questionnaire is a suitable tool to measure the physical activity of pregnant women and can be used in clinical practice.
Biology of the pine spittlebug, Aphrophora flavipes (Homoptera:Cercopidae) in the nymphal stage.

マツアワフキAphrophora flavipesの幼虫期の生態を,東京大学演習林田無試験地(東京都田無市)に植栽された6年生のアカマツ12本,クロマツ13本で1984年に調査した。 幼虫は4月末から5月末にかけて孵化し,孵化開始後約60日で最初の成虫が出現した。 幼虫の頭盾幅を測定したところ,齢間で重なりが見られたが,体格も同時に考慮すると5齢期を区別できた。 幼虫の作る泡は新梢と1年枝に限って見られ,前者ではとくに基部に初期の幼虫が,後者では末端部に後期の幼虫が多く存在した。 一つの泡にしばしば複数の幼虫が潜み,平均値は終始1より有意に大きく,幼虫には集合する傾向があった。 枝階別の垂直分布を調べると,6月に入ってから大きな変化があり,幼虫が枝から枝へ移動していることが示唆された。 枝階に基づいて層化して求めた枝1本あたりの平均密度は5月21・22日頃にピークに達し,アカマツで7. 8匹,クロマツで6. 7匹であった。 RICHARDS-WALOFF法によって求めた枝1本あたりの孵化個体数は,アカマツで10. 5(木1本あたりでは290匹),クロマツで14. 0(木1本あたり328匹)であった。 1日あたり生存率はアカマツで0. 990,クロマツで0. 970と比較的高かった。 調査木上に見いだされた捕食性昆虫は泡の中に侵入せず,捕食例は確認できなかった。
Effect of Ultraviolet C Irradiation on Isoflavone Concentrations in Different Cultivars of Soybean (Glycine max)

Phytoestrogens are naturally occurring plant polyphenolic compounds present in high concentrations in soybean products. Phytoestrogens are divided into three classes: lignans, isoflavones, and coumestans. Nine types of glycoside isoflavones and three types of aglycoside isoflavones are reported in soybean. Soy isoflavones can reduce the risk of a certain type of cancer, cardiovascular problems, osteoporosis, and menopausal symptoms. We irradiated the leaves of five cultivars of soybean with UV-C (260 nm) and determined the effect on concentrations of isoflavone compounds using liquid chromatography–mass spectrometry (LC–MS). Isoflavone concentrations were significantly higher following irradiation, particularly in the cultivar Daepung, which was selected as the best cultivar for high isoflavone induction with UV-C irradiation. Further experimentation with the cultivar Daepung revealed that 20 min UV-C irradiation was the best treatment for the induction of aglycone compounds, and 5 min with the dorsal surface facing the UV-C irradiation source was the best treatment for the induction of glycoside isoflavone compounds.
Are Myths and Preconceptions Preventing Us from Applying Ionic Liquid Forms of Antiviral Medicines to the Current Health Crisis?

At the moment, there are no U.S. Food and Drug Administration (U.S. FDA)-approved drugs for the treatment of COVID-19, although several antiviral drugs are available for repurposing. Many of these drugs suffer from polymorphic transformations with changes in the drug’s safety and efficacy; many are poorly soluble, poorly bioavailable drugs. Current tools to reformulate antiviral APIs into safer and more bioavailable forms include pharmaceutical salts and cocrystals, even though it is difficult to classify solid forms into these regulatory-wise mutually exclusive categories. Pure liquid salt forms of APIs, ionic liquids that incorporate APIs into their structures (API-ILs) present all the advantages that salt forms provide from a pharmaceutical standpoint, without being subject to solid-state matter problems. In this perspective article, the myths and the most voiced concerns holding back implementation of API-ILs are examined, and two case studies of API-ILs antivirals (the amphoteric acyclovir and GSK2838232) are presented in detail, with a focus on drug property improvement. We advocate that the industry should consider the advantages of API-ILs which could be the genesis of disruptive innovation and believe that in order for the industry to grow and develop, the industry should be comfortable with a certain element of risk because progress often only comes from trying something different.
Vaginal and Penile Microbiome Associations With Herpes Simplex Virus Type 2 in Women and Their Male Sex Partners

Abstract Background We determined how the vaginal and penile microbiomes contribute to herpes simplex virus type 2 (HSV-2) serostatus within sexual partnerships. Methods Microbiomes were characterized in cervicovaginal lavage and penile meatal swab specimens through high-throughput 16s ribosomal RNA gene amplicon sequencing. HSV-2 antibody was detected in serum specimens. We modeled vaginal and penile taxa and covariates contributing to HSV-2 status in women and men using bivariate probit analysis. Results Among 231 couples, HSV-2 was detected in both partners in 78 couples (33.8%), in the woman only in 52 (22.5%),in the man only in 27 (11.7%), and in neither in 74 (32.0%). Among the women (median age, 22 years) 10.9% had human immunodeficiency virus (HIV), and 21.4% had Bacterial vaginosis. Among men (median age, 26 years), 11.8% had HIV, and 55.0% circumcised. In an analysis with adjustment for sociodemographics and Bacterial vaginosis, enrichment of vaginal Gardnerella vaginalis and Lactobacillus iners was associated with increased likelihood of HSV-2 in both partners. Penile taxa (including Ureaplasma and Aerococcus) were associated with HSV-2 in women. Conclusions We demonstrate that penile taxa are associated with HSV-2 in female partners, and vaginal taxa are associated with HSV-2 in male partners. Our findings suggest that couples-level joint consideration of genital microbiome and sexually transmitted infection or related outcomes could lead to new avenues for prevention.
Quantitative Estimation of Lactoflavin and of Vitamin B6 in Cow's Milk and in Human Milk

It has been shown 1 that vitamin G as needed by the rat contains at least 2 major components: lactoflavin and vitamin B6, the lack of the latter being associated with specific skin lesions (“rat pellagra” or, rather, “rat acrodynia” 2 ). By feeding to rats a vitamin B-free diet complemented by vitamin B proper, the antineuritic factor (B1), and lactoflavin, or by vitamin B1 and a vitamin B6 concentrate, it was possible to obtain quantitative data2, 3 concerning the distribution of lactoflavin and vitamin B6 in different foodstuffs. For the estimation of the lactoflavin values, the basal vitamin B-free diet was supplemented by crystalline (or highly purified) vitamin B1 (3 international units daily) plus vitamin B6 concentrate. For the estimation of vitamin B6 values, vitamin B1 was similarly provided but supplemented by pure lactoflavin (10 γ daily). The vitamin B6 concentrate consisted of a yeast preparation such as may be obtained according to the method of Peters, et al., 4 by adsorption of a yeast extract on charcoal and subsequent elution with alcohol containing hydrochloric acid. It has been found that 1 cc. of this so-called Peters' eluate is equivalent to about 1 “rat-day dose” of vitamin B6. The values for lactoflavin were determined by means of the growth test, skin symptoms being too irregular and frequently insufficiently obvious. The “rat-day dose,” taken as provisional unit, was defined as the minimum quantity of the substance which would give rise to a gain in weight of about 10 (9 to 11) gm. per week for at least 4 weeks. The values for vitamin B6 were based directly on the curative effect against dermatitis.
A French–Greek Cross-Site Comparison Study of the Use of Automatic Video Analyses for the Assessment of Autonomy in Dementia Patients

Background: At present, the assessment of autonomy in daily living activities, one of the key symptoms in Alzheimer’s disease (AD), involves clinical rating scales. Methods: In total, 109 participants were included. In particular, 11 participants during a pre-test in Nice, France, and 98 participants (27 AD, 38 mild cognitive impairment—MCI—and 33 healthy controls—HC) in Thessaloniki, Greece, carried out a standardized scenario consisting of several instrumental activities of daily living (IADLs), such as making a phone call or preparing a pillbox while being recorded. Data were processed by a platform of video signal analysis in order to extract kinematic parameters, detecting activities undertaken by the participant. Results: The video analysis data can be used to assess IADL task quality and provide clinicians with objective measurements of the patients’ performance. Furthermore, it reveals that the HC statistically significantly outperformed the MCI, which had better performance compared to the AD participants. Conclusions: Accurate activity recognition data for the analyses of the performance on IADL activities were obtained.
Multiple Retinal Anomalies in Wfs1-Deficient Mice

Background: Wolfram syndrome (WFS, OMIM: #222300) is an ultrarare autosomal recessive disorder characterized by diabetes insipidus, diabetes mellitus, optic nerve atrophy and deafness. It has been reported that the average retinal thickness in WFS patients decreases with the progression of the disease. Aim: To investigate retinal thickness and wolframin expression disorders in Wolfram syndrome 1 gene knockout (Wfs1KO) mice compared to their wild-type (WT) littermates. Materials and methods: Both bulbs with optic nerves of three mice Wfs1WT and three Wfs1KO were taken for the histopathological examination. A strain of knockout mice with mutation in exon 8 was used. Results: No expression of wolframin protein in the retina and neurodegeneration of the optic nerve of Wfs1KO mice as compared among Wfs1WT mice was observed. The mean central retinal thickness was thinner and the retinal thickness/longitudinal diameter ratio was significantly lower in hte Wfs1KO as compared to the Wfs1WT mice. In four (67%) eyeballs of Wfs1KO mice, intra-retinal neovessels were observed. Conclusions: Wfs1KO mice retina with mutation in exon 8 present similar clinical features as patients with WFS in the form of reduced retinal thickness and neurodegeneration of the optic nerve. The presence of proliferative retinopathy observed in Wfs1KO mice requires further investigation.
Preventing HIV outbreaks among people who inject drugs in the United States: Plus ça change, plus ça même chose.

: This editorial review covers current trends in the epidemiology of HIV among people who inject drugs (PWID) in the United States, including four recent HIV outbreaks. We discuss gaps in the prevention and treatment cascades for HIV and medications for opioid disorder and propose lessons learned to prevent future HIV outbreaks. Over the last decade, North America has been in the throes of a major opioid epidemic, due in part to over-prescribing of prescription opiates, followed by increasing availability of cheap heroin, synthetic opioids (e.g. fentanyl), and stimulants (e.g. methamphetamine). Historically, HIV infection among PWID in the US had predominantly affected communities who were older, urban and Black. More recently, the majority of these infections are among younger, rural or suburban and Caucasian PWID. All four HIV outbreaks were characterized by a high proportion of women who inject drugs and underlying socioeconomic drivers such as homelessness and poverty. We contend that the US response to the HIV epidemic among PWID has been fractured. A crucial lesson is that when evidence-based responses to HIV prevention are undermined or abandoned because of moral objections, untold humanitarian and financial costs on public health will ensue. Restructuring a path forward requires that evidence-based interventions be integrated and brought to scale while simultaneously addressing underlying structural drivers of HIV and related syndemics. Failing to do so will mean that HIV outbreaks among PWID and the communities they live in will continue to occur in a tragic and relentless cycle.
Disposable and Low-Cost Electrode Based on Graphene Paper-Nafion-Bi Nanostructures for Ultra-Trace Determination of Pb(II) and Cd(II)

There is a huge demand for rapid, reliable and low-cost methods for the analysis of heavy metals in drinking water, particularly in the range of sub-part per billion (ppb). In the present work, we describe the preparation, characterization and analytical performance of the disposable sensor to be employed in Square Wave Anodic Stripping Voltammetry (SWASV) for ultra-trace simultaneous determination of cadmium and lead. The electrode consists of graphene paper-perfluorosulfonic ionomer-bismuth nano-composite material. The electrode preparation implies a key step aimed to enhance the Bi3+ adsorption into nafion film, prior to the bismuth electro-deposition. Finely dispersed bismuth nanoparticles embedded in the ionomer film are obtained. The electrode was characterized by Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDX), Atomic Force Microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and Electrochemical Impedance Spectroscopy (EIS). The electrode shows a linear response in the 5–100 ppb range, a time-stability tested up to almost three months, and detection limits up to 0.1 ppb for both Pb2+ and Cd2+. The electrode preparation method is simple and low in cost and the obtained analytical performance is very competitive with the state of art for the SWASV determination of Pb2+ and Cd2+ in solution.
Prognostic Significance of Incidental Deep Vein Thrombosis in Patients with Cancer Presenting with Incidental Pulmonary Embolism

In symptomatic acute pulmonary embolism (PE), the presence of deep vein thrombosis (DVT) is a risk factor for 30- and 90-day mortality. In patients with cancer and incidental PE, the prognostic effect of concomitant incidental DVT is unknown. In this retrospective study, we examined the effect of incidental DVT on all-cause mortality in such patients. Adjusted Cox multivariate regression analysis was used for relevant covariates. From January 2010 to March 2018, we included 200 patients (mean age, 65.3 ± 12.4 years) who were followed up for 12.5 months (interquartile range 7.4–19.4 months). Of these patients, 62% had metastases, 31% had concomitant incidental DVT, and 40.1% (n = 81) died during follow-up. All-cause mortality did not increase in patients with DVT (hazard ratio [HR] 1.01, 95% confidence interval [CI] 0.43–2.75, p = 0.855). On multivariate analysis, weight (adjusted HR 0.96, 95% CI 0.92–0.99, p = 0.032), and metastasis (adjusted HR 10.26, 95% CI 2.35–44.9, p = 0.002) were predictors of all-cause mortality. In conclusion, low weight and presence of metastases were associated with all-cause mortality, while presence of concomitant DVT was unrelated to poorer survival.