Miracle-dz/UniFAR-FTUs huggingface dataset:
Dietary rapeseed meal and the incidence of tainted eggs

1. Incorporating 0, 3, 6 or 9% rapeseed meal in the diet of brown‐egg laying birds for 28 d resulted in the production of 0, 1.2, 19.3 and 20.9% tainted eggs respectively, the first tainted eggs being laid on the fifth day. 2. During the second and third weeks the incidence of tainted eggs exceeded 20% but fell to 11.4% during the final week. 3. Omission of the rapeseed meal from the diets halted the production of tainted eggs. 4. Neither egg production nor the health of the birds was adversely affected by the treatments. 5. The taint was described as “ fishy ” or “ crabby ” and was distinctive, but the source was not identified.
Chemical Composition and Nutritive Value of Dimaru (Ficus Glomerata Roxb.) Leaves

Dimaru (Ficus Glomerata Roxb.) leaves contained 13.56 CP, 2.94 EE, 21.15 CF, 46.16 NFE, 15.84 TA, 3.67 CA and 0.75 P on per cent DM basis. The nutritive value E. glomarata leaves for cattle was 7.89 per cent DCP, 55.85 per cent TDN and 43.02 per cent SE.
gd T cells present antigen to CD4 1 ab T cells

gd and ab TCR 1 T cells share many properties and their interactions are likely to be coordinated and regulated. We provide evidence that cattle gd T cells are able to present antigen to CD4 1 T cells. To help elucidate their function gd T cell lines were propagated for extensive character- ization. Cells expressed high levels of MHC class II and production of co-stimulatory molecules as evidenced by the binding of a CTLA-4 fusion protein and synthesis of CD80 transcripts. These properties and the presence of a well-developed endosomal compartment indicated the cells might function as antigen-presenting cells. Resting CD4 1 T cells from calves immunized with ovalbumin or respiratory syncytial virus-antigen proliferated in response to gd T cells pulsed with antigen that appeared to be endocytosed via clathrin-coated pits or specific receptors. J. Leukoc. Biol. 63: 707- 714; 1998.
Cerebral Dopamine Neurotrophic Factor Regulates Multiple Neuronal Subtypes and Behavior

Cerebral dopamine neurotrophic factor (CDNF) protects dopaminergic neurons against toxic damage in the rodent brain and is in clinical trials to treat Parkinson's disease patients. Yet the underlying mechanism is poorly understood. To examine its significance for neural circuits and behavior, we examined the development of neurotransmitter systems from larval to male adult mutant zebrafish lacking cdnf. Although a lack of cdnf did not affect overall brain dopamine levels, dopaminergic neuronal clusters showed significant abnormalities. The number of histamine neurons that surround the dopaminergic neurons was significantly reduced. Expression of tyrosine hydroxylase 2 in the brain was elevated in cdnf mutants throughout their lifespan. There were abnormally few GABA neurons in the hypothalamus in the mutant larvae, and expression of glutamate decarboxylase was reduced throughout the brain. cdnf mutant adults showed a range of behavioral phenotypes, including increased sensitivity to pentylenetetrazole-induced seizures. Shoaling behavior of mutant adults was abnormal, and they did not display social attraction to conspecifics. CDNF plays a profound role in shaping the neurotransmitter circuit structure, seizure susceptibility, and complex behaviors in zebrafish. These findings are informative for dissecting the diverse functions of this poorly understood factor in human conditions related to Parkinson's disease and complex behaviors. SIGNIFICANCE STATEMENT A zebrafish lacking cdnf grows normally and shows no overt morphologic phenotype throughout the life span. Remarkably, impaired social cohesion and increased seizure susceptibility were found in adult cdnf KO fish conceivably associated with significant changes of dopaminergic, GABAergic, and histaminergic systems in selective brain areas. These findings suggest that cdnf has broad effects on regulating neurogenesis and maturation of transmitter-specific neuronal types during development and throughout adulthood, rather than ones restricted to the dopaminergic systems.
Validation of the scale for assessing the psychological vulnerability and its association with health of intimate partner violence victims in Chinese young adult population

The Women’s Experience with Battering (WEB) scale is a self-report instrument that uses a 10-item Likert-type scale to measure IPV victims’ cognitive and affective experience of battering. This study aimed to validate the Chinese version of the WEB scale using gender-neutral questions, Experience of Battering Scale (Chinese) (EBS-C), to assess the psychological vulnerability of victims of intimate partner violence (IPV). The study adopted a range of methods, including translation and back translation, expert reviews, cognitive debriefing, and test-retest reliability assessment. The EBS-C was validated in a purposive convenience sample of 718 Chinese-speaking participants (male = 362; female = 356) aged 18–24 (mean age = 21.4) in Hong Kong. The results of CFA showed a good model fit: CFI = . 97, TLI = . 96, RMSEA = . 05, SRMR = . 03. The EBS-C was also found to be significantly associated with the Revised Conflict Tactics Scales (CTS2; r = .13–.17, p < .01), depression (BDI-II: r = .15, p < .01), anxiety (GAD-7: r = .17, p < .01), interpersonal support (ISEL-12: r = -.27, p < .01), relationship satisfaction (RAS: r = -.36, p < .01), and self-esteem (RSES: r = -.22, p < .01). The study demonstrated the EBS-C to be a reliable and valid measure for assessing the psychological vulnerability of IPV victims. It is thus useful for identifying the risks such individuals face by assessing their experience of fear, danger, and disempowerment in the intimate relationship relative to abusive incident-based measures alone. The EBS-C will also be useful for developing effective treatments to address the psychological vulnerability resulting from IPV and will facilitate cross-cultural comparative research aimed at enriching our knowledge of IPV victimization.
MiR-126 regulated myocardial autophagy on myocardial infarction.

OBJECTIVE The aim of the study is to detect the effect of miR-126 in autophagy after acute myocardial infarction (AMI). MATERIALS AND METHODS First, the changes of miR-126 and autophagy in myocardial infarction tissue and normal heart tissue were compared by establishing the myocardial infarction rat model, so as to clarify the relationship between miR-126 and changes of myocardial infarction and autophagy. Second, the regulation of miR-126 on autophagy related protein Beclin-1 and the role in myocardial infarction were studied to clarify whether miR-126 regulates autophagy through Beclin-1 and participates in the occurrence and development of myocardial infarction. Finally, the relationship between plasma miR-126 was observed. RESULTS MiR-126 expression can regulate Beclin-1 expression and influence the cardiac function after AMI. The results are of great significance to reveal the new mechanism of myocardial infarction. CONCLUSIONS The down-regulation of miR-126 will lead to the over-activation of myocardial autophagy induced by Beclin-1, which is an autophagy related protein. The plasm expression of miR-126 may be a clinical marker of autophagy after AMI.
Profiling the Expression of Genes Related to Ethylene Biosynthesis, Ethylene Perception, and Cell Wall Degradation during Fruit Abscission and Fruit Ripening in Apple

polygalacturonase ABSTRACT.The expressionofgenes forethylene biosynthesis,ethylene perception, andcell walldegradationin thefruit cortex and fruit abscission zone (FAZ) was examined in relation to preharvest fruit abscission (PFA) and fruit ripening in 'Golden Delicious' and 'Fuji' apple (Malus ·domestica Borkh.). PFA, fruit ethylene production, and fruit softening increased rapidly during fruit ripening in 'Golden Delicious' apples, whereas no PFA, little fruit ethylene, and gradual fruit softening were recorded in 'Fuji' apples. The transcript levels of 1-aminocyclopropane-1- carboxylate (ACC) synthase genes, MdACS1, MdACS3, and MdACS5A, increased rapidly in the fruit cortex of 'Golden Delicious' apples during ripening, but not in 'Fuji' apples. However, only the level of MdACS5A mRNA was up-regulated in the FAZ of 'Golden Delicious' apples. The transcript level of ACC oxidase gene, MdACO1, increased in the fruit cortex for both cultivars but increased only in the FAZ of 'Golden Delicious' apples. Expression of the ethylene receptor genes, MdETR1, MdETR2, MdERS1, and MdERS2, increased in the fruit cortex for both cultivars, but only MdETR2 and MdERS2 increased in the FAZ of 'Golden Delicious' apples. The transcript levels of MdPG2, a polygalacturonase gene (PG), and MdEG1 ,a b-1,4-glucanase gene, markedly increased only in the FAZ of 'Golden Delicious' apples, whereas only MdPG1 rapidly increased in the fruit cortex of 'Golden Delicious' apples. Our results suggested that MdACS5A, MdACO1, MdPG2, and MdEG1 in the FAZ might be related to the difference in PFA between these two cultivars, whereas MdACS1 and MdPG1 were associated with fruit softening.
Deciphering the Binding of Salicylic Acid to Arabidopsis thaliana Chloroplastic GAPDH-A1

Salicylic acid (SA) has an essential role in the responses of plants to pathogens. SA initiates defence signalling via binding to proteins. NPR1 is a transcriptional co-activator and a key target of SA binding. Many other proteins have recently been shown to bind SA. Amongst these proteins are important enzymes of primary metabolism. This fact could stand behind SA’s ability to control energy fluxes in stressed plants. Nevertheless, only sparse information exists on the role and mechanisms of such binding. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was previously demonstrated to bind SA both in human and plants. Here, we detail that the A1 isomer of chloroplastic glyceraldehyde 3-phosphate dehydrogenase (GAPA1) from Arabidopsis thaliana binds SA with a KD of 16.7 nM, as shown in surface plasmon resonance experiments. Besides, we show that SA inhibits its GAPDH activity in vitro. To gain some insight into the underlying molecular interactions and binding mechanism, we combined in silico molecular docking experiments and molecular dynamics simulations on the free protein and protein–ligand complex. The molecular docking analysis yielded to the identification of two putative binding pockets for SA. A simulation in water of the complex between SA and the protein allowed us to determine that only one pocket—a surface cavity around Asn35—would efficiently bind SA in the presence of solvent. In silico mutagenesis and simulations of the ligand/protein complexes pointed to the importance of Asn35 and Arg81 in the binding of SA to GAPA1. The importance of this is further supported through experimental biochemical assays. Indeed, mutating GAPA1 Asn35 into Gly or Arg81 into Leu strongly diminished the ability of the enzyme to bind SA. The very same cavity is responsible for the NADP+ binding to GAPA1. More precisely, modelling suggests that SA binds to the very site where the pyrimidine group of the cofactor fits. NADH inhibited in a dose-response manner the binding of SA to GAPA1, validating our data.
Manufacturing an assistive device with 3D printing technology – a case report

ABSTRACT Our aim is to define the design and procedure of a 3D printed assistive device and to produce a prototype assistive device for an individual with quadriplegia in order to enable him/her to independently conduct daily living activities. For the design and production of the 3D assistive device, we followed six steps. In the first step-determination of problematic activity/client-centered approach-writing activity was determined as the most problematic activity as a result of the interview with a paraplegic patient. Then, 2D drawings of the assistive device were created as the second step. For the third step the 3D scan of the individual’s right hand was obtained. 3D scan of the right hand was transferred to CATIA program and the 3D printer is used in the manufacturing process for the fourth step. In the fifth step, we produced a low-cost novel writing assistive device. The writing device was introduced to the disabled individual and training was given regarding its practical use in the final step teaching the use of the device. As 3D printing technology progresses and becomes more reliable, we believe that the production of assistive devices will become much easier and their use will become more prevalent.
Sexual Harassment Experiences, Knowledge, and Coping Behaviors of Nursing Students in Taiwan During Clinical Practicum

Sexual harassment not only endangers nursing students’ physical and mental health but also considerably affects their future willingness to engage in the field of nursing. To identify experiences, knowledge, coping behaviors, and determinants of sexual harassment among nursing students during clinical practicum, this study conducted a cross-sectional survey where a structured self-report questionnaire was used. A total of 291 senior nursing students were recruited from four universities in Central Taiwan. Sixty-six nursing students (22.7%), including 59 women (23.3%) and 7 men (18.4%), reported experiencing sexual harassment during clinical practicum. Male students scored significantly higher than female students did on knowledge of sexual harassment (p = 0.028). Female students scored significantly higher than male students did on attitudes toward preventing and coping with sexual harassment (p = 0.05). Nursing students who were older, had fathers who had higher education levels, or had undergone gender-related courses were more likely to experience sexual harassment. More than one-fifth of nursing students experienced sexual harassment during their clinical practicum, making this a formidable challenge in nursing education. Education is required to prevent sexual harassment and enhance gender sensitivity among nursing students, who are at a greater risk of experiencing sexual harassment in clinical practicum.
Feature Sensing and Robotic Grasping of Objects with Uncertain Information: A Review

As there come to be more applications of intelligent robots, their task object is becoming more varied. However, it is still a challenge for a robot to handle unfamiliar objects. We review the recent work on the feature sensing and robotic grasping of objects with uncertain information. In particular, we focus on how the robot perceives the features of an object, so as to reduce the uncertainty of objects, and how the robot completes object grasping through the learning-based approach when the traditional approach fails. The uncertain information is classified into geometric information and physical information. Based on the type of uncertain information, the object is further classified into three categories, which are geometric-uncertain objects, physical-uncertain objects, and unknown objects. Furthermore, the approaches to the feature sensing and robotic grasping of these objects are presented based on the varied characteristics of each type of object. Finally, we summarize the reviewed approaches for uncertain objects and provide some interesting issues to be more investigated in the future. It is found that the object’s features, such as material and compactness, are difficult to be sensed, and the object grasping approach based on learning networks plays a more important role when the unknown degree of the task object increases.
MOOSE HABITAT PREFERENCES IN RESPONSE TO CHANGING AVAILABILITY

Abstract Application of Habitat Suitability Index (HSI) models without testing in areas other than where they were generated, and claims that habitat preferences have been proven, indicate that managers and scientists believe that habitat preferences of wildlife are fixed. We tested this hypothesis by comparing habitat preferences of 2 groups of moose (Alces alces) in northeastern Alberta, Canada, to which the same habitat classes were available but differed in relative abundance. We estimated habitat availability for each of 22 radiomarked, adult female moose and divided the animals into 2 groups based on the similarity of relative habitat class abundances. We measured habitat preference for individual moose from each group during 2 seasons in each of 2 years using a simple resource selection function (RSF). We used analysis of variance (ANOVA) to compare differences between groups. Preference of several habitat classes differed between groups, indicating that habitat preferences of moose are not fixed and change as the relative abundance of available habitat changes. Managers must recognize and account for this concept in the application of habitat prescriptions or management plans.
Genome annotation across species using deep convolutional neural networks

Application of deep neural network is a rapidly expanding field now reaching many disciplines including genomics. In particular, convolutional neural networks have been exploited for identifying the functional role of short genomic sequences. These approaches rely on gathering large sets of sequences with known functional role, extracting those sequences from whole-genome-annotations. These sets are then split into learning, test and validation sets in order to train the networks. While the obtained networks perform well on validation sets, they often perform poorly when applied on whole genomes in which the ratio of positive over negative examples can be very different than in the training set. We here address this issue by assessing the genome-wide performance of networks trained with sets exhibiting different ratios of positive to negative examples. As a case study, we use sequences encompassing gene starts from the RefGene database as positive examples and random genomic sequences as negative examples. We then demonstrate that models trained using data from one organism can be used to predict gene-start sites in a related species, when using training sets providing good genome-wide performance. This cross-species application of convolutional neural networks provides a new way to annotate any genome from existing high-quality annotations in a related reference species. It also provides a way to determine whether the sequence motifs recognised by chromatin-associated proteins in different species are conserved or not.
Biological mechanisms of ectopic lymphoid structure formation and their pathophysiological significance

Abstract Ectopic lymphoid structures (ELS) or tertiary lymphoid organs are structures with the organization similar to the one of secondary lymphoid organs, formed in non-lymphoid tissues. They are considered to be an important site for the lymphocytic physiological and pathological role in conditions such are chronic infections, autoimmune diseases, cancer, and allograft rejection. Although similar to the secondary lymphoid tissues, the initiation of ELS formation is not preprogramed and requires chronic inflammation, expression of homeostatic chemokines, and lymphotoxin beta receptor activation. Importantly, while ELS formation may be considered beneficiary in antimicrobial and antitumor immunity, the persistence of these active lymphoid structures within the tissue increase the chance for development of autoimmunity and lymphoma. This paper is providing an overview of biological mechanisms involved in ELS formation, as well as the overview of the pathophysiological role of these structures. In addition, the paper discusses the possibility to therapeutically target ELS formation, bearing in mind their bivalent nature and role in different pathophysiological conditions.
Identification of QTLs for Resistance to Fusarium Head Blight Using a Doubled Haploid Population Derived from Southeastern United States Soft Red Winter Wheat Varieties AGS 2060 and AGS 2035

Fusarium head blight (FHB), caused primarily by the fungus Fusarium graminearum, is one of the most damaging diseases of wheat, causing significant loss of yield and quality worldwide. Warm and wet conditions during flowering, a lack of resistant wheat varieties, and high inoculum pressure from corn stubble contribute to frequent FHB epidemics in the southern United States. The soft red winter wheat variety AGS 2060 is moderately susceptible (as opposed to susceptible) to FHB and regularly found in pedigrees of resistant breeding lines. AGS 2060 does not carry any known resistance genes or quantitative trait loci (QTL). A QTL mapping study was conducted to determine the location and genetic effect of its resistance using a doubled haploid mapping population produced from a cross between wheat varieties AGS 2060 and AGS 2035 (FHB susceptible). The population was genotyped using the Illumina iSelect single nucleotide polymorphism (SNP) array for wheat and phenotyped in Baton Rouge and Winnsboro, Louisiana and Newport, Arkansas in 2018 and 2019. The effect of genotype was significant for Fusarium damaged kernels (FDK) and deoxynivalenol (DON) content across all locations and years, indicating genetic variation in the population. The study detected 13 QTLs (one each on chromosome 1A, 1B, 1D, 2A, 2B, 6A, 6B, 7A, and 7B, and two each on 5A and 5B) responsible for the reduction of FDK and/or DON. Of these, nine QTLs for FHB resistance were identified in Winnsboro, Louisiana, in 2019. QTLs on chromosomes 2A and 7A could be valuable sources of resistance to both DON and FDK over several environments and were likely the best candidates for use in marker-assisted selection. Consistently expressed QTLs on chromosomes 5A, 6B, and 7A were potentially newly identified sources of resistance to FHB in soft red winter wheat.
Biometrical Studies on Iraqi Honeybees

SummaryBiometrical studies on the yellow, aggressive Iraqi worker honeybees gave the following averages: weight 90·0 mg; length of tongue 5·3 mm; length and width of fore-wing 8·8 and 2·9 mm; cubital index 1·95; length and width of hind-wing 6·6 and 1·7 mm; number of wing hooks 21·1; length and width of hind basitarsus 2·0 and 1·1 mm; length and width of second wax mirror 2·1 and 1·4 mm.
Chemoenzymatic Synthesis of D-Glucitol-Based Non-Ionic Amphiphilic Architectures as Nanocarriers

Newer non-ionic amphiphiles have been synthesized using biocompatible materials and by following a greener approach i.e., D-glucitol has been used as a template, and hydrophobic and hydrophilic segments were incorporated on it by using click chemistry. The hydrophilic segments in turn were prepared from glycerol using an immobilized Candida antarctica lipase (Novozym-435)-mediated chemoenzymatic approach. Surface tension measurements and dynamic light scattering studies reflect the self-assembling behavior of the synthesized amphiphilic architectures in the aqueous medium. The results from UV-Vis and fluorescence spectroscopy establish the encapsulation of guests in the hydrophobic core of self-assembled amphiphilic architectures. The results of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay indicate that the amphiphiles are well tolerated by the used A549 cell lines at all tested concentrations.