Miracle-dz/UniFAR-FTUs huggingface dataset:
Abstract C145: Compounds that inhibit the activation of Grb2 by EGFR, HER2, and HER3 complexes.

Purpose: To demonstrate that ORIL003 (Glc4-Rha2-Glc-diosgenin; full structure will be disclosed) inhibits the activation of Grb2 by EGFR, HER2 and HER3 complexes using the Receptor-Heteromer Investigation Technology (Receptor-HIT) on the bioluminescence resonance energy transfer (BRET) platform. Introduction: The unique property of inhibiting a range of receptor tyrosine kinases (RTKs) in the cell plasma membrane has been demonstrated in a family of steroid saponins. As the compounds have a pan-inhibitory effect, they are effective in inhibiting a wide range of cancer cell types, including lung, breast, prostate, colorectal, melanoma, ovarian and leukemia. Methods: Receptor-HIT, previously published in the form of G protein-coupled receptor heteromer identification technology (GPCR-HIT; See et al, 2011 Assay Drug Dev Technol 9:21–30), was used to determine close proximity and activation of ErbB growth factor receptors and Grb2. EGFR, HER2 and HER3 were tagged with Rluc8 (a variant of Renilla luciferase) as donor; Grb2 was tagged with GFP2 (a variant of green fluorescent protein) as acceptor. Human embryonic kidney 293 cells were stimulated with either epidermal growth factor (EGF) or heregulin (HRG) at a concentration of 20–50 nM. ORIL003 (as an example of the steroid saponin family) was applied to the cells at a concentration of 10 μM, which was known from in vitro studies to completely inhibit cancer cell growth. Results: Stimulation by EGFR or HRG resulted in recruitment of Grb2. This recruitment was inhibited by the use of ORIL003 in a dose-dependent manner. Furthermore, constitutive interactions between the ErbB growth factor receptors and Grb2 also appear to be inhibited by ORIL003. Conclusions: ORIL003 is effective in inhibiting the activation of Grb2 by EGFR, HER2 and HER3 complexes. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2011 Nov 12-16; San Francisco, CA. Philadelphia (PA): AACR; Mol Cancer Ther 2011;10(11 Suppl):Abstract nr C145.
Bacteriological studies in sediment from Cerna-Belareca-Danube hydrographic system (SW Romania) with a role in assessing pollution.

This paper contains data about the microorganism population in sediment from Cerna-Belareca-Danube hydrographic system. Data allows us to identify possible pollution sources and determine their effect on certain eco physiological group of microorganisms. Bacteriological studies had in view to establish three eco physiological groups of microorganism: nitrifying bacteria, iron-reducing bacteria and anaerobic sulfate-reducing bacteria. Determination has been made using serial dilutions and specific culture environment for each eco physiological group. Based on absolute values of those 3 microorganism groups, the bacterial indicator of sediment quality has been established (BISQ). Sediment analyses have values between 1.274 (Orsova upstream) and 1.296 (Mehadia downstream) in spring. These are the values for winter: 1.286 (Topleţ downstream) and 1.310 (Orsova upstream). Additional contribution of organic sulfur compounds and microorganisms involved in the sulfur cycle have relatively close values for both seasons in Herculane sample point. Sampling points Mehadia downstream and Topleţ downstream represent the endpoint of BISQ. Lowest values had been recorded here in autumn and the highest values had been recorded in spring. BISQ lower values from Orsova harbor and Orsova shipyard is due to oil pollution, varnishes, paints, detergents and metals. BISQ has seasonal variations, depending on the sampling point of the studied aquatic ecosystem.
The cerebrum and cerebellum of the fixed human brain: efficient and unbiased estimates of volumes and cortical surface areas.

Abstract Extremely old and relatively new stereological methods for the efficient and unbiased estimation of volumes and surface areas were applied to fixed human brains. Brains from twelve subjects (six males aged 76-81 years, six females aged 70-98 years) were hemisected. Cerebral hemispheres and cerebellar halves from both sides were sliced systematic randomly for Cavalieri estimates of volume and vertical sectioning estimates of cortical surface area. Weights and linear dimensions were also recorded. It took less than 30 minutes per cerebral hemisphere to estimate total volume and cortical surface area. Cerebellar halves were analysed even more quickly. No significant differences between brain sides and no interaction effects were found but sex differences were confirmed. For male cerebrum (both hemispheres combined), the average volume was 840 cm3 and cortical surface area was 1640 cm2. Two thirds of this surface was hidden within sulci and in the insula. Cortical volume was 320 cm2 with an arithmetic mean thickness of 2.2 mm. In females the cerebral hemisphere was smaller and the cortex was less extensive but just as voluminous. In males, the cerebellum occupied 70 cm3 with a cortical surface of 550 cm2 of which 86% was hidden in fissures. Values were not significantly different from those found in females.
Year-round use of coastal lakes by marbled murrelets

Sixty-seven records (from 1909 to 1984) of Marbled Murrelets (Brachyramphus marmoratus) using 33 freshwater lakes along the west coast of North America (78.6% in British Columbia, 12.1% in Alaska, 6.1% in Washington, and 3.0% in Oregon) were compiled and analyzed. The preponderance of records from southern British Columbia lakes possibly reflects the abundance of coastal lakes there that are accessible to high numbers of murrelets and observers. Fifty-three of the lake-use records were obtained during the species' breeding season (April through September); 13 were from the nonbreeding season (October to March). Most lakes used were within 20 km of the ocean, but a few murrelets were reported at lakes 50 to 75 km inland (Cultus, Harrison, and Swan Lakes, British Columbia). The multiple records of Marbled Murrelets using Cultus and Harrison Lakes suggest that these lakes are near nesting areas, and as such, are the farthest distances inland Marbled Murrelets can be expected to nest. Five or less individuals usually were reported in each record, although up to 46 were seen in summer on Nitinat Lake, Vancouver Island. Feeding occurred on some lakes in British Columbia, apparently mainly at night: the stomachs of four murrelets contained fingerling sockeye salmon (Oncorhynchus nerka), while another stomach contained the remains of salmon fry.
An experimental 4-Mbit CMOS EEPROM with a NAND-structured cell

A 5-V-only CMOS 512 K*8 EEPROM (electrically erasable and programmable read-only memory), which achieves 10/sup 4/ cycle endurance using a NAND-structured cell, is discussed. The main features are a programming technique appropriate to the NAND structured cell and a dynamic sense amplifier. The NAND structured cell arranges 8 bits in series, sandwiched between two select transistors. The first transistor ensures selectivity; the second prevents the current from passing during programming operation. The current cell has one select transistor per bit. However, the NAND structure has only two select transistors per 8 bits; therefore it has only 1/4 select transistor and 1/16 contact hole per bit. Thus, the NAND structure can realize a smaller cell area than that of the current cell. For high-speed programming, a page mode is adopted. In read operation, address transition detection circuitry is used to precharge the bit lines and reset the read control circuitry. A summary of design characteristics is presented. >
Cultured Fish Species Affected by Red Sea Bream Iridoviral Disease from 1991 to 1995

1991年から1995年にかけて海産養殖魚において“マダイイリドウイルス病”の発生した都道府県数と発生魚種をまとめた。 ギムザ染色による本病発生の確認は, 1991年には12県7魚種であったが, 1994年および1995年は発生魚種が急増した。 ギムザ染色または単クローン抗体を用いた蛍光抗体法により1995年までに本病の発生が確認された都道府県数と魚種は, 17府県20魚種(スズキ目18, カレイ目1, フグ目1)にのぼった。
Ultrasound elastography using a regularized modified error in constitutive equations (MECE) approach: a comprehensive phantom study

Many of the current techniques in transient elastography, such as shear wave elastography (SWE) assume a dominant planar shear wave propagating in an infinite medium. This underlying assumption, however, can be easily violated in real scenarios in vivo, leading to image artifacts and reconstruction errors. Other approaches that are not bound to planar shear wave assumption, such solutions based on the partial differential equation, can potentially overcome the shortcomings of the conventional SWE. The main objective of this paper is to demonstrate the advantages of the modified error in constitutive equations (MECE) formulation with total variation regularization (MECE + TV) over SWE in reconstructing the elastic moduli of different tissue-mimicking phantoms. Experiments were conducted on phantoms with inclusions of well-defined shapes to study the reconstruction of specific features relevant to practical applications. We compared the performances of MECE + TV and SWE in terms of quantitative metrics to estimate reconstruction accuracy, inclusion shape recovery, edge preservation and edge sharpness, inclusion size representation, and shear elasticity and contrast accuracies. The results indicate that the MECE + TV approach outperforms SWE based on several of these metrics. It is concluded that, with further development, the proposed method may offer elastography reconstructions that are superior to SWE in clinical applications.
Job burnout and turnover intention among Chinese primary healthcare staff: the mediating effect of satisfaction

Objectives Although China has done a lot in strengthening the primary healthcare system, the high turnover intention is still a social problem to be reckoned with. The objective of this study is to explore the mediating effect of satisfaction between job burnout and turnover intention. Design Cross-sectional study. Methods A cross-sectional study was conducted to make sense of the job burnout, satisfaction and turnover intention among primary healthcare workers in central China. Structural equation modelling (SEM) was performed to study the mediating effect of satisfaction between job burnout and turnover intention with maximum likelihood estimation. The mediation effect test was carried out by using the bootstrap method. Results SEM showed that job burnout was positively related to the turnover intention with the standard path coefficient of 0.845 (C.R.=34.055, p<0.001). The partial mediating effect of satisfaction was 0.047, making up 5.32% of the total effect. The goodness-of-fit was acceptable (Goodness of Fit Index=0.947, Comparative Fit Index=0.975, root mean square error of approximation=0.067, Non-Normed Fit Index=0.971, Incremental Fit Index=0.975). Age, education level, monthly income, hire form and night shift were also found significantly correlated with turnover intention, and no difference was found between physicians and nurses. Conclusions The turnover intention is significantly affected by job burnout, satisfaction and demographical characteristics including age, education level, monthly income, hire form and night shift. Satisfaction can be regarded as a mediator between job burnout and turnover intention. Relative measures can be taken to promote enthusiasm and satisfaction thus decreasing the turnover rate.
Nanoparticulated Systems Based on Natural Polymers Loaded with Miconazole Nitrate and Lidocaine for the Treatment of Topical Candidiasis

People with weakened immune systems are at risk of developing candidiasis which is a fungal infection caused by several species of Candida genus. In this work, polymeric nanoparticles containing miconazole nitrate and the anesthetic lidocaine clorhydrate were developed. Miconazole was chosen as a typical drug to treat buccopharyngeal candidiasis whereas lidocaine may be useful in the management of the pain burning, and pruritus caused by the infection. Nanoparticles were synthesized using chitosan and gelatin at different ratios ranging from 10:90 to 90:10. The nano-systems presented nanometric size (between 80 and 300 nm in water; with polydispersion index ranging from 0.120 to 0.596), and positive Z potential (between 20.11 and 37.12 mV). The determined encapsulation efficiency ranges from 65 to 99% or 34 to 91% for miconazole nitrate and lidocaine clorhydrate, respectively. X-ray diffraction and DSC analysis suggested that both drugs were in amorphous state in the nanoparticles. Finally, the systems fitted best the Korsmeyer–Peppas model showing that the release from the nanoparticles was through diffusion allowing a sustained release of both drugs and prolonged the activity of miconazole nitrate over time against Candida albicans for at least 24 h.
Systemic social and emotional learning: Promoting educational success for all preschool to high school students.

Social and emotional learning (SEL) has become more central to education because of demand from educators, parents, students, and business leaders alongside rigorous research showing broad, positive impacts for students and adults. However, all approaches to SEL are not equal. Systemic SEL is an approach to create equitable learning conditions that actively involve all Pre-K to Grade 12 students in learning and practicing social, emotional, and academic competencies. These conditions require aligned policies, resources, and actions at state and district levels that encourage local schools and communities to build the personal and professional capacities of adults to: implement and continuously improve evidence-based programs and practices; create an inclusive culture that fosters caring relationships and youth voice, agency, and character; and support coordinated school-family-community partnerships to enhance student development. Promoting social and emotional competencies-including the abilities to understand and manage emotions, achieve positive goals, show caring and concern for others, establish and maintain positive relationships, and make responsible decisions-are important for success at school and in life. In this article, we summarize key concepts and evidence for systemic SEL. Next, we explain interrelated Theories of Action and resources developed by the Collaborative for Academic, Social, and Emotional Learning (CASEL) to implement and continuously improve systemic SEL in schools, districts, and states. We discuss research on nested, interacting settings and processes involved in systemic SEL at proximal (classrooms, schools, families, and communities) and distal (districts, states, national, and international) ecological levels. We conclude with recommendations for future SEL research, practice, and policy. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
Overexpression of endothelial β3‐adrenergic receptor induces diastolic dysfunction in rats

Diastolic dysfunction is common in cardiovascular diseases, particularly in the case of heart failure with preserved ejection fraction. The challenge is to develop adequate animal models to envision human therapies in the future. It has been hypothesized that this diastolic dysfunction is linked to alterations in the nitric oxide (•NO) pathway. To investigate this issue further, we investigated the cardiac functions of a transgenic rat model (Tgβ3) that overexpresses the human β3‐adrenoceptor (hβ3‐AR) in the endothelium with the underlying rationale that the •NO pathway should be stimulated in the endothelium.
Pharmaceutical importance of zinc and metallothionein in cell signalling

Zinc as an essential element plays the crucial role in many physiological, but also pathological processes - zinc controls cell proliferation, differentiation, and viability including the apoptosis. These facts are especially based on the structural role of zinc ions in many proteins including the transcription factors. Due to the role of zinc in many crucial physiological processes, its homeostasis must be intensively controlled. The controlling mechanisms are based especially on the zinc transporters and low-molecular proteins metallothioneins (MTs) of the molecular weight between 6 and 10 kDa, which were firstly discovered in a horse kidneys as the cadmium-binding protein. High affinity of metallothioneins to the zinc ions indicates their significant participation in the physiological, but also pathological processes, which are controlled by zinc. This paper summarizes the connection between metallothioneins and zinc and its significance on the molecular basis.
Exercise in older women with breast cancer during systemic therapy: study protocol of a randomised controlled trial (BREACE)

Introduction Exercise interventions have been widely investigated in patients with cancer and demonstrate beneficial effects. However, intervention studies that include older women with breast cancer exercising during medical treatment are scarce. Hence, the aim of this study is to investigate the effect of a 12-week exercise-based intervention in older women (≥65 years) with breast cancer receiving (neo)adjuvant or first-line or second-line systemic therapy. Methods and analysis This is a single-centre, two-armed randomised controlled trial. We anticipate including 100 patients, who will be randomised 1:1 to exercise-based intervention or control stratified by treatment setting ((neo)adjuvant or metastatic) and treatment (chemotherapy or endocrine therapy + cyclin-dependent kinase (CDK) 4/6 inhibitors). The intervention group will receive standard oncological treatment and a 12-week supervised exercise-based intervention comprising a progressive resistance exercise programme two times per week, a protein supplement after exercise and a home-based walking programme based on daily step counts. The control group will receive standard oncological treatment. Assessments will be performed at baseline and 6, 12 and 24 weeks after start of the intervention. Primary outcome is physical function, measured by the 30-second Chair Stand Test. Secondary outcomes are feasibility (compliance and adherence to intervention), objective and patient-reported functional measures (6-meter and 10-meter gait speed; 6-min Walk Test; Handgrip Strength; Stair Climb Test; Physical Activity Questionnaire), symptom burden and well-being (MD Anderson Symptom Inventory; Hospital Anxiety and Depression Scale), quality of life (European Organization for Research and Treatment of Cancer Quality of Life Questionnaire Core-30 and B23), body composition (dual-energy X-ray absorptiometry scan), side effects, inflammatory biomarkers, hospitalisation and survival. Ethics and dissemination The protocol was reviewed and accepted by the Scientific Ethics Review Committee of the Capital Region of Denmark, 17 June 2018 (VEK ref.: H-18021013). Trial results will be submitted for publication in a peer-reviewed journal and presented on conferences, in oncology wards, exercise centres in municipalities and patient organisations, ensuring dissemination to relevant stakeholders. Trial registration number https://clinicaltrials.gov/ on 3 September 2018. Identifier: NCT03656731.
Global and regional dispersal patterns of hepatitis B virus genotype E from and in Africa: A full-genome molecular analysis

Description of the spatial characteristics of viral dispersal is important in understanding the history of infections. Nine hepatitis B virus (HBV) genotypes (A-I), and a putative 10th genotype (J), with distinct geographical distribution, are recognized. In sub-Saharan Africa (sub)-genotypes A1, D3 and E circulate, with E predominating in western Africa (WA), where HBV is hyperendemic. The low genetic diversity of genotype E (HBV/E) suggests its recent emergence. Our aim was to study the dispersal of HBV/E using full-length, non-redundant and non-recombinant sequences available in public databases. HBV/E was confirmed, and the phylogeny reconstruction performed using maximum likelihood (ML) with bootstrapping. Phylogeographic analysis was conducted by reconstruction of ancestral states using the criterion of parsimony on the estimated ML phylogeny. 46.5% of HBV/E sequences were found within monophyletic clusters. Country-wise analysis revealed the existence of 50 regional clusters. Sequences from WA were located close to the root of the tree, indicating this region as the most probable origin of the HBV/E epidemic and expanded to other geographical regions, within and outside of Africa. A localized dispersal was observed with sequences from Nigeria and Guinea as compared to other WA countries. Based on the sequences available in the databases, the phylogenetic results suggest that European strains originated primarily from WA whereas a majority of American strains originated in Western Central Africa. The differences in regional dispersal patterns of HBV/E suggest limited cross-border transmissions because of restricted population movements.
Neuronal lipolysis participates in PUFA‐mediated neural function and neurodegeneration

Lipid droplets (LDs) are dynamic cytoplasmic organelles present in most eukaryotic cells. The appearance of LDs in neurons is not usually observed under physiological conditions, but is associated with neural diseases. It remains unclear how LD dynamics is regulated in neurons and how the appearance of LDs affects neuronal functions. We discovered that mutations of two key lipolysis genes atgl‐1 and lid‐1 lead to LD appearance in neurons of Caenorhabditis elegans. This neuronal lipid accumulation protects neurons from hyperactivation‐triggered neurodegeneration, with a mild decrease in touch sensation. We also discovered that reduced biosynthesis of polyunsaturated fatty acids (PUFAs) causes similar effects and synergizes with decreased lipolysis. Furthermore, we demonstrated that these changes in lipolysis and PUFA biosynthesis increase PUFA partitioning toward triacylglycerol, and reduced incorporation of PUFAs into phospholipids increases neuronal protection. Together, these results suggest the crucial role of neuronal lipolysis in cell‐autonomous regulation of neural functions and neurodegeneration.
Adjunctive bright light therapy for treating bipolar depression: A systematic review and meta‐analysis of randomized controlled trials

Bright light therapy (BLT) was reported as an effective adjunctive treatment option for bipolar disorder. Previous meta‐analytic study showed that augmentation treatment with light therapy significantly decreased the severity of bipolar depression. However, most of included studies were case–control studies and several of them focused on BLT that was provided in combination with sleep deprivation therapy.
Analysis of Breeding Situation and Countermeasures of Procambarus clarkia

Procambarus clarkia industry has a good economic benefit and market prospect and Procambarus clarkia is a potential breed variety.The author found there were some problems about its developing process.With the reduction of wildlife groups,the quality of population is retrograde;the breeding modes are dated,all kinds of diseases occur frequently and the development of forage lags behind.Therefore,the author thinks that the development of Procambarus clarkia industry should protect wildlife resources and select high quality varieties through cross breeding;and use the mode of breeding many times one year or alternate breeding with other shrimp to solve the problem of low efficiency in the pond.Then,various aquatic plants should be grown in order to reduce the incidence rate of the disease and develop the proprietary forage of Procambarus clarkia.By saving the capital and improving the technique,a sustainable development of Procambarus clarkia industry can be achieved.