Miracle-dz/UniFAR-FTUs huggingface dataset:
Study on Antimicrobial Activity of Extracts from Physalis alkekengi L.var.franchetii(Mast.)Makino

In order to know antimicrobial spectrum of extracts of Physalis alkekengi.var. francheti(Mast.)Makino,antibacterial effect test in vitro was done by disk diffusion method.The result showed that the extracts can inhibit Staphylococcus aureus,alpha streptococcus,beta streptococcus,beta streptococcus,Bacillus subtilis,but can not inhibit Bacillus aeruginosus,Bacillus coli,Staphylococcus epidermidis,Staphylococcus pythogenesis,Blastomyces albicans.It shows that there is effective antimicrobial component in the extracts of Physalis alkekengi.var. francheti(Mast.)Makino,which is highly sensitive to Staphylococcus aureus,alpha streptococcus,beta streptococcus in the extracts of Physalis alkekengi.var. francheti(Mast.)Makino.And the MIC of the refined extract ranges from 25～50 g/L.
Bloch Lines Constituting Antiskyrmions Captured via Differential Phase Contrast

Much scientific capital has been directed toward exotic magnetic spin textures called Bloch lines, that is, Néel‐type line boundaries within domain walls, because their geometry promises high‐density magnetic storage. While predicted to arise in high‐anisotropy magnets, bulk soft magnets, and thin films with in‐plane magnetization, Bloch lines also constitute magnetic antiskyrmions, that is, topological antiparticles of skyrmions. Most domain walls occur as Bloch‐type or Néel‐type, in which the magnetization rotates parallel or perpendicular to the domain wall across its profile, respectively. The Bloch lines’ Néel‐type rotation and their minute size make them difficult to directly measure. This work utilizes differential phase contrast (DPC) scanning transmission electron microscopy (STEM) to measure the in‐plane magnetization of Bloch lines within antiskyrmions emergent in a non‐centrosymmetric Heusler magnet with D2d symmetry, Mn1.4Pt0. 9Pd0.1Sn, in addition to Bloch‐type skyrmions in an FeGe magnet with B20‐type crystal structure to benchmark the DPC technique. Both in‐focus measurement and identification of Bloch lines at the antiskyrmion's corners are provided.
Dietary supplement use in Korean children and adolescents, KNHANES 2015–2017

Abstract Objective: To date, there have been few studies on dietary supplement (DS) use in Korean children and adolescents, using nationally representative data. This study aimed to investigate the current status of DS use and its related factors, among Korean children and adolescents from the Korean National Health and Nutrition Examination Survey (KNHANES) data. Design: A cross-sectional study. Setting: Data from the KNHANES 2015–2017. Participants completed 24-h dietary recall interviews, including DS products that the subjects consumed. Participants: The study population was 4380 children and adolescents aged 1–18 years. Results: Approximately 2013 % of children and adolescents were using DS; the highest use was among children aged 1–3 years old, and the lowest use was among adolescents aged 16–18 years. The most frequently used DS was prebiotics/probiotics, followed by multivitamin/mineral supplements. Factors that were associated with DS use were lower birth weight in children aged <4 years; younger age, higher household income, regular breakfast intake and lower BMI in children aged 4–9 years; and regular breakfast intake and use of nutrition facts label in adolescents aged 10–18 years. Feeding patterns in infancy and having chronic diseases were not associated with DS use. Conclusions: We report that over 20 % of children and adolescents use DS. Nutritional education for parents and children about proper DS consumption is needed.
Astragaloside IV prevents myocardial hypertrophy induced by mechanical stress by activating autophagy and reducing inflammation.

AIMS The aim of the present study was to investigate the effects of astragaloside IV (As-IV) on mechanical stress-induced myocardial hypertrophy, with a focus on autophagy and inflammation. METHODS A rat cardiac hypertrophy model was established by narrowing the abdominal aorta, and a cell hypertrophy model was established by mechanically stretching primary cardiomyocytes. Cardiac function index and cardiac hypertrophy were measured by echocardiography, heart weight index (HWI) and left ventriculus weight index (LVWI) in vivo. Cell size was measured by phalloidin-tetramethyl treatment in vitro, while hematoxylin and eosin (HE) staining was used to observe the arrangement and morphology of myocardial cells. The expression of ANP, BNP, LC3II, p62, NLRP3, and IL-1β in both myocardial tissue and cardiomyocytes was assessed by Western blot, while TNF-α and IL-18 levels in serum and cell supernatants were measured by ELISA. RESULTS In the aortic banding model, the cardiac function index LVEF was decreased; the hypertrophy indexes LVPWd, LVPWs, IVSd and IVSs were significantly increased; cardiomyocytes were enlarged and disordered; the expression levels of ANP, BNP, NLRP3, IL-1β and p62 were increased; and LC3II expression was decreased in both myocardial tissue and cardiomyocytes. As-IV could significantly improve cardiac function and cardiomyocyte morphology and limit hypertrophy, thereby protecting damaged hearts, while rapamycin had a similar effect as As-IV. In addition, As-IV decreased the expression of NLRP3 and IL-1β and activated autophagy, as evidenced by increased LC3II expression and decreased p62 levels. CONCLUSION As-IV prevents myocardial hypertrophy induced by mechanical stress by activating autophagy and reducing inflammation.
The functions of EZH2 in immune cells: Principles for novel immunotherapies

Enhancer of zeste homolog 2 (EZH2) is aberrantly expressed or mutated in multiple types of cancer cells and plays an oncogenic role in tumorigenesis and development in most cancers. Results from pilot clinical studies have implied that EZH2 inhibitors have therapeutic potential against some cancers. However, the exact mechanisms by which EZH2 plays oncogenic roles and EZH2 inhibition exerts anticancer effects are incompletely understood. To date, the findings of studies focusing on EZH2 and cancer cells have failed to fully explain the observations in preclinical and clinical studies. Therefore, recent studies about the roles of EZH2 in cancers have shifted from cancer cells to immune cells. The human immune system is a complex network comprising multiple subpopulations of immune cells. Immune cells communicate and interact with cancer cells during cancer development and treatment, dictating the fate of cancer cells. Elucidating the roles of EZH2 in immune cells, especially in cancer patients, promises the identification of novel immunotherapeutic strategies or priming of existing immunotherapies against cancer. Hence, we reviewed the studies focusing on the involvement of EZH2 in various immune cells, aiming to provide ideas for immunotherapies targeting EZH2 in immune cells.
Identification of acute kidney injury subphenotypes

Purpose of review AKI is a complex clinical syndrome with many causes and there is a broad range of clinical presentations that vary according to duration, severity and context. Established consensus definitions of AKI are nonspecific and limited to kidney function. This reduces treatment options to generic approaches rather than individualized, cause-based strategies that have limited both understanding and management of AKI. Recent findings The context and the temporal phase of kidney injury are critical features in the course of AKI and critical to timing-relevant intervention. These features are missing in generic definitions and terms used to describe AKI. Subphenotypes of AKI can be identified from novel damage biomarkers, from functional changes including creatinine trajectories, from the duration of change and from associated clinical characteristics and comorbidities. Subphenotype parameters can be combined in risk scores, or by association strategies ranging from a simple function-damage matrix to complex methods, such as machine learning. Examples of such strategies are reviewed along with tentative proposals for a revised nomenclature to facilitate description of AKI subphenotypes. Summary Appropriate intervention requires refinement of the nomenclature of AKI to identify subphenotypes that facilitate correctly timed and selectively targeted intervention.
SiRNA targeting PFK1 inhibits proliferation and migration and enhances radiosensitivity by suppressing glycolysis in colorectal cancer.

PURPOSE This study explored the effects of phosphofructokinase-1 (PFK1) on the radiosensitivity of colorectal cancer (CRC) in vivo and in vitro and the underlying mechanisms. METHODS Tissue samples from 48 patients with rectal cancer who had received neoadjuvant radiotherapy followed by surgery were analyzed. The expression of PFK1 in tissue samples was semi-quantitated by immunohistochemistry, and its relationship with clinicopathological features was analyzed. The effects of PFK1 knockdown on the survival, apoptosis, migration, and radiosensitivity of CRC cells were evaluated. Glycolysis-related indicators were used to examine glycolytic activity. The effects of PFK1 on the radiosensitivity of CRC in vivo were assessed by measuring tumor formation in nude mice. RESULTS PFK1 was overexpressed in rectal cancer and was higher in radiation-resistant tumors than in radiation-sensitive tumors. SiRNA-induced PFK1 silencing increased apoptosis and inhibited migration and proliferation of CRC cells. Knockdown of PFK1 made the CRC cells sensitive to ionizing radiation in vivo. Oligomycin partially restored the expression of PFK1, enhanced glycolysis, and reversed the enhanced radiosensitivity of CRC cells induced by siRNA-PFK1. Downregulation of PFK1 combined with irradiation inhibited growth of nude mice xenografts, which was related to an increase in apoptosis. CONCLUSIONS Our study indicates that high expression of PFK1 is negatively correlated with radiosensitivity in CRC and likely accelerates the proliferation and migration of CRC cells. Downregulation of PFK1 may enhance the radiosensitivity of CRC cells in vivo and in vitro by inhibiting glycolysis.
Endothelial Dysfunction and Platelet Hyperaggregation in type 2 Diabetes Mellitus: The era of novel anti-diabetic agents.

BACKGROUND Diabetes mellitus (DM) incidence is ever-increasing and along with its microvascular and macrovascular complications is associated with a high morbidity and mortality burden globally. Major components of diabetes pathophysiology include glucotoxicity, lipotoxicity and insulin resistance, disturbing the vascular wall integrity and leading to endothelial dysfunction and platelet hyperaggregation. OBJECTIVE This review aims to identify and summarize the effect of novel anti-diabetic agents (glucagon-like peptide-1 receptor agonists, dipeptidyl peptidase-4 inhibitors, sodium-glucose co-transporter -2 inhibitors) on endothelial (EF) and platelet function (PF) and evaluate the consistency with the results of cardiovascular outcomes studies. METHODS We performed a structured search of the PubMed database for peer-reviewed research of the literature between 1981 and 2020 regarding the effect of DM and novel anti-diabetic agents on EF and PF. RESULTS We analyzed data regarding the effect of novel anti-diabetic agents on EF and PF as well as the pathophysiological interplay between DM, PF, and EF. The available studies use different methods to evaluate these outcomes and the results of different studies are rather conflicting as a result of different study designs, combinations of drugs tested, small study samples and patient population heterogeneity. CONCLUSION The currently available data do not unequivocally support a consistent effect of novel antidiabetic agents on EF and PF. Further study is required ideally with validation of the results with clinical outcomes.
Fitbit-Based Interventions for Healthy Lifestyle Outcomes: Systematic Review and Meta-Analysis

Background Unhealthy behaviors, such as physical inactivity, sedentary lifestyle, and unhealthful eating, remain highly prevalent, posing formidable challenges in efforts to improve cardiovascular health. While traditional interventions to promote healthy lifestyles are both costly and effective, wearable trackers, especially Fitbit devices, can provide a low-cost alternative that may effectively help large numbers of individuals become more physically fit and thereby maintain a good health status. Objective The objectives of this meta-analysis are (1) to assess the effectiveness of interventions that incorporate a Fitbit device for healthy lifestyle outcomes (eg, steps, moderate-to-vigorous physical activity, and weight) and (2) to identify which additional intervention components or study characteristics are the most effective at improving healthy lifestyle outcomes. Methods A systematic review was conducted, searching the following databases from 2007 to 2019: MEDLINE, EMBASE, CINAHL, and CENTRAL (Cochrane). Studies were included if (1) they were randomized controlled trials, (2) the intervention involved the use of a Fitbit device, and (3) the reported outcomes were related to healthy lifestyles. The main outcome measures were related to physical activity, sedentary behavior, and weight. All the studies were assessed for risk of bias using Cochrane criteria. A random-effects meta-analysis was conducted to estimate the treatment effect of interventions that included a Fitbit device compared with a control group. We also conducted subgroup analysis and fuzzy-set qualitative comparative analysis (fsQCA) to further disentangle the effects of intervention components. Results Our final sample comprised 41 articles reporting the results of 37 studies. For Fitbit-based interventions, we found a statistically significant increase in daily step count (mean difference [MD] 950.54, 95% CI 475.89-1425.18; P<.001) and moderate-to-vigorous physical activity (MD 6.16, 95% CI 2.80-9.51; P<.001), a significant decrease in weight (MD −1.48, 95% CI −2.81 to −0.14; P=.03), and a nonsignificant decrease in objectively assessed and self-reported sedentary behavior (MD −10.62, 95% CI −35.50 to 14.27; P=.40 and standardized MD −0.11, 95% CI −0.48 to 0.26; P=.56, respectively). In general, the included studies were at low risk for bias, except for performance bias. Subgroup analysis and fsQCA demonstrated that, in addition to the effects of the Fitbit devices, setting activity goals was the most important intervention component. Conclusions The use of Fitbit devices in interventions has the potential to promote healthy lifestyles in terms of physical activity and weight. Fitbit devices may be useful to health professionals for patient monitoring and support. Trial Registration PROSPERO International Prospective Register of Systematic Reviews CRD42019145450; https://www.crd. york.ac. uk/prospero/display_record. php? ID=CRD42019145450
Distal Radial Artery Access for Superficial Femoral Artery Interventions

Purpose: To compare the acute success and complication rates of distal radial (DR) vs proximal radial (PR) artery access for superficial femoral artery (SFA) interventions. Materials and Methods: Between 2016 and 2019, 195 consecutive patients with symptomatic SFA stenosis were treated via DR (n=38) or PR (n=157) access using a sheathless guide. Secondary access was achieved through the pedal artery when necessary. The main outcomes were technical success, major adverse events (MAEs), and access site complications. Secondary outcomes were treatment success, fluoroscopy time, radiation dose, procedure time, and crossover rate to another puncture site. Results: Overall technical success was achieved in 188 patients (96.4%): 37 of 38 patients (97.3%) in the DR group and 151 of 157 patients (96.2%) in the PR group (p=0.9). Dual (transradial and transpedal) access was used in 14 patients (36.8%) in the DR group and 28 patients (18.9%) in the PR group (p<0.01). Chronic total occlusions were recanalized in 25 of 26 DR patients (96.1%) and in 79 of 81 PR patients (92.6%) (p=0.57). The crossover rate to femoral access was 0% in the DR group vs 3.2% in the PR group (p=0.59). Stents were implanted in the SFA in 15 DR patients (39.4%) and in 39 patients (24.8%) in the PR group (p=0.1). The contrast volume, fluoroscopy time, radiation dose, and procedure time were not statistically different between the DR and PR groups, nor were the rates of access site complications (2.6% and 7.0%, respectively). The cumulative incidences of MAE at 6 months in the DR and PR groups were 15.7% vs 14.6%, respectively (p=0.8). Conclusion: SFA interventions can be safely and effectively performed using PR or DR access with acceptable morbidity and a high technical success rate. DR access is associated with few access site complications.
The impact of Tai Chi on cognitive rehabilitation of elder adults with mild cognitive impairment: a systematic review and meta-analysis

Abstract Purpose To systematically research the impact of Tai Chi intervention on cognitive rehabilitation of elderly adults with mild cognitive impairment (MCI). Materials and methods Both English and Chinese databases were systematically searched from inception to December 2019: MEDLINE, EMBASE, the Cochrane Library, Web of Science, SinoMed database, China National Knowledge Infrastructure, Traditional Chinese Medicine clinical evidence database, Wanfang knowledge database, Chinese Science and Technology Periodical Database. Randomized controlled trials (RCTs) and quasi-experimental studies that involved older adults with MCI, Tai Chi intervention, and cognitive rehabilitation outcomes were included. The meta-analysis was conducted and reported according to PRISMA guidelines (PROSPERO registration number CRD42019124925). Results Twelve studies were included. For the meta-analysis, Tai Chi exercise had a large beneficial effect on global cognitive ability and long-term delayed recall ability from the memory domain. In terms of executive ability, Tai Chi intervention had a moderate and significant effect on improving patients’ executive function. Conclusions The results suggest a Tai Chi intervention lasting at least 12 weeks with a frequency of three times per week and 30–60 min per session. Tai Chi shows promise as an alternative mind-body intervention for MCI rehabilitation among elder patients. IMPLICATIONS FOR REHABILITATION From a global perspective, Tai Chi may be an effective mind-body exercise for cognitive rehabilitation among elder patients with MCI, which is not restricted by the condition of equipment, manpower, and venue. This review suggests an intervention that includes a warm-up, exercise, and cool-down period and lasts at least 12 weeks with a frequency of three times per week and 30–60 min per session. It is also recommended to choose suitable training methods according to local conditions, including one-to-one coaching, group training, VCD, and visual reality technology for some developed regions.
A qualitative study of the views about smoking, licensed cessation aids and e-cigarettes in people with schizophrenia spectrum disorders

Abstract Smoking prevalence remains high among people with a mental health condition compared to those without. Understanding people’s motivation to quit and their views of smoking cessation support may help to develop future interventions. We conducted a qualitative study to explore the perceptions of 30 people with schizophrenia spectrum disorders who were current smokers, about smoking traditional cigarettes, the appeal of licensed medicines and e-cigarettes for smoking cessation or smoking reduction. The experiences of participants who were motivated to quit were compared with those who were not motivated to quit. Findings suggest traditional cigarettes were pleasurable and licensed cessation aids and e-cigarettes unappealing to participants who were unmotivated to quit. Whereas nicotine replacement products and e-cigarettes may be an appealing smoking cessation or reduction strategy for those motivated to quit. There is a need to find ways of making traditional cigarettes less appealing and alternative less harmful nicotine products (licensed and unlicensed) more appealing and accessible to this group of high risk smokers.
Radiomic features derived from pre-operative multi-parametric MRI of prostate cancer are associated with Decipher risk score

Decipher, a genomic test, is used to predict the likelihood of metastasis and prostate cancer (PCa) specific mortality based on expression patterns of 22 RNA markers from radical prostatectomy (RP) specimens. It has been shown to be strongly correlated with metastasis-free prognosis and has been integrated with the National Comprehensive Cancer Network (NCCN) guidelines. However, Decipher is expensive and tissue destructive. Radiomic features refer to the high-throughput computational texture or shape features extracted from radiographic scans. Radiomic features derived from multi-parametric magnetic resonance imaging (mpMRI) of prostate cancer have been shown to be associated with clinically significant PCa. In this study, we sought to evaluate whether radiomic features derived from T2-weighted MRI (T2WI) and apparent diffusion coefficient (ADC) maps of the prostate could distinguish different Decipher risk groups (low, intermediate and high). We also explored correlations between Decipher risk associated radiomic features and features relating to gland morphology on corresponding digitized surgical specimens. A retrospectively acquired, de-identified cohort of 70 PCa patients (N = 74 lesions) who underwent 3T mpMRI prior to RP and Decipher tests after RP were used in this study. The Decipher risk score, ranging from 0 to 1, was used to categorize patients into low/intermediate (D1) and high (D2) risk groups. A multivariate logistic regression model was trained (N = 37 lesions) using radiomic features selected via elastic-net regularization to predict the Decipher risk groups. The model was evaluated on a hold-out test set (N = 37 lesions) and resulted in an area under the receiver operating characteristic curve (AUC) = 0:80. Our model outperformed the prediction using PIRADS v2 (AUC = 0:67), but showed comparable performance with Gleason Grade Group (GGG) (AUC = 0:80). We observed that the best discriminating radiomic features were correlated with gland morphology and gland packing on corresponding histopathology (R = 0.43, p < 0.05).
Large animal models for translational research in acute kidney injury

Abstract While extensive research using animal models has improved the understanding of acute kidney injury (AKI), this knowledge has not been translated into effective treatments. Many promising interventions for AKI identified in mice and rats have not been validated in subsequent clinical trials. As a result, the mortality rate of AKI patients remains high. Inflammation plays a fundamental role in the pathogenesis of AKI, and one reason for the failure to translate promising therapeutics may lie in the profound difference between the immune systems of rodents and humans. The immune systems of large animals such as swine, nonhuman primates, sheep, dogs and cats, more closely resemble the human immune system. Therefore, in the absence of a basic understanding of the pathophysiology of human AKI, large animals are attractive models to test novel interventions. However, there is a lack of reviews on large animal models for AKI in the literature. In this review, we will first highlight differences in innate and adaptive immunities among rodents, large animals, and humans in relation to AKI. After illustrating the potential merits of large animals in testing therapies for AKI, we will summarize the current state of the evidence in terms of what therapeutics have been tested in large animal models. The aim of this review is not to suggest that murine models are not valid to study AKI. Instead, our objective is to demonstrate that large animal models can serve as valuable and complementary tools in translating potential therapeutics into clinical practice.