Miracle-dz/UniFAR-FTUs huggingface dataset:
A novel mutant Na+/HCO3− cotransporter NBCe1 in a case of compound‐heterozygous inheritance of proximal renal tubular acidosis

The inheritance of two defective alleles of SLC4A4, the gene that encodes the widely‐expressed electrogenic sodium bicarbonate cotransporter NBCe1, results in the bicarbonate‐wasting disease proximal renal tubular acidosis (pRTA). In the present study, we report the first case of compound‐heterozygous inheritance of pRTA (p.Arg510His/p.Gln913Arg) in an individual with low blood pH, blindness and neurological signs that resemble transient ischaemic attacks. We employ fluorescence microscopy on non‐polarized (human embryonic kidney) and polarized (Madin–Darby canine kidney) renal cell lines and electrophysiology on Xenopus oocytes to characterize the mutant transporters (R510H and Q913R). Both mutant transporters exhibit enhanced intracellular retention in renal cells, an observation that probably explains the HCO3− transport deficit in the individual. Both mutants retain a close‐to‐normal per molecule Na+/HCO3− cotransport activity in Xenopus oocytes, suggesting that they are suitable candidates for folding‐correction therapy. However, Q913R expression is uniquely associated with a depolarizing, HCO3− independent, Cl−‐conductance in oocytes that could have pathological consequences if expressed in the cells of patients.
Rapid Turnover of c-FLIPshort Is Determined by Its Unique C-terminal Tail*

The caspase-8 inhibitor c-FLIP exists as two splice variants, c-FLIPL and c-FLIPS, with distinct roles in death receptor signaling. The mechanisms determining their turnover have not been established. We found that in differentiating K562 erythroleukemia cells both c-FLIP isoforms were inducibly degraded by the proteasome, but c-FLIPS was more prone to ubiquitylation and had a considerably shorter half-life. Analysis of the c-FLIPS-specific ubiquitylation revealed two lysines, 192 and 195, C-terminal to the death effector domains, as principal ubiquitin acceptors in c-FLIPS but not in c-FLIPL. Furthermore the c-FLIPS-specific tail of 19 amino acids, adjacent to the two target lysines, was demonstrated to be the key element determining the isoform-specific instability of c-FLIPS. Molecular modeling in combination with site-directed mutagenesis demonstrated that the C-terminal tail is required for correct positioning and subsequent ubiquitylation of the target lysines. Because the antiapoptotic operation of c-FLIPS was not affected by the tail deletion, the antiapoptotic activity and ubiquitin-mediated degradation of c-FLIPS are functionally and structurally independent processes. The presence of a small destabilizing sequence in c-FLIPS constitutes an important determinant of c-FLIPS/c-FLIPL ratios by allowing differential degradation of c-FLIP isoforms. The conformation-based predisposition of c-FLIPS to ubiquitin-mediated degradation introduces a novel concept to the regulation of the death-inducing signaling complex.
The financial crisis in the National Health Service: : insufficient funding will derail transformation plans

NHS England’s Five Year Forward View (5YFV) set out ambitious plans to transform the National Health Service (NHS) by 2021. The plans required the NHS to achieve productivity gains of 2-3% a year and the government to increase NHS funding by £8bn. Even though NHS productivity growth has outstripped that of the economy as a whole since the recession, the gains required of the 5YFV are unprecedented. For its part, the government is committing just £4.5bn in increased funding and is also pushing for a 7-day NHS which will further increase costs. Meanwhile the NHS is in financial crisis,, with growing hospital deficits diverting resources from the 5YFV’s transformation agenda. All told, the ambitious plans of the 5YFV are unlikely to be realised.
Towards Automated Intelligence in 5G Systems

In this paper, we call for a paradigm shift away from the wireless-access focused research efforts on 5G networked systems. We believe that the architectural limitations should share equal blame on issues of performance, reliability, and security. We thus identify architectural weakness on both sides of the mobile clients and the 4G network infrastructure. Our recent findings show that, contrary to commonly held perceptions, many design and operational issues arise not due to poor wireless link qualities. Instead, they are rooted in such architectural downsides. To address these issues, we further propose a new approach of enabling automated intelligence inside the 4G/5G network systems. We next describe our ongoing efforts along two dimensions: empowering date-driven smart clients and constructing verifiable network infrastructure. We report some early results and discuss possible next steps.
PACS through Web Compatible with DICOM Standard and WADO Service: Advantages and Implementation

All users of informatics applications need rapid and reliable access to the kind of information that they are interested in. Web technology provides these capabilities. DICOM standard committees recognized the necessity of a Web medical standard. They specified WADO (Web access to DICOM object) service, so that system interaction takes place through Web, in a standarized way, allowing interoperability and proper information management inside PACS. The advantages of a Web PACS comparatively with a compatible PACS are multiple and they are detected in different fields of functionality. The authors have run a project of a WADO compatible Web PACS development. A Web portal platform with enhanced security has been implemented. Over it, DICOM applications have been developed. JavaServer Pages (JSP) technology is mainly used to satisfy design specifications and dynamic data exchanges. Furthermore, Java applets have been developed and introduced in the whole project to serve specific demands. Evaluation results confirmed our considerations about the improvement of DICOM services, when they are provided through Web
28S rDNA evolution in the Eumalacostraca and the phylogenetic position of krill.

The Malacostraca are an ancient and morphologically diverse class of Crustacea. The phylogenetic position of one order within this class, the Euphausiacea ("krill," subclass Eumalacostraca) was investigated using 28S rDNA sequences from representatives of several malacostracan orders. Phylogenies for these sequences were estimated by maximum-likelihood and maximum-parsimony analysis. The results of these analyses produced a new scheme for evolution within the Eumalacostraca. The new phylogenies suggested that Euphausiacea are most closely related to the Mysida and not the Decapoda, as is generally thought. Furthermore, the Mysida were found not to be closely related to the Lophogastrida, which are often considered their sister taxon. These hypotheses were tested against the hypotheses of monophyly for the Eucarida, Mysidacea, and Peracarida and found to be significantly better on the basis of the 28S rDNA data.
Management InformationSystem:Few TipsBefitting SmallBusinessestoAvertID TheftCritical of Illicit Technology

Carrying large sums of money everywhere has long been seen cumbersome and provocative practice to criminals. Later this practice was viewed unprofessional within business and corporate practice and was thus replaced by cheques and lately by bank cards loosely referred to as plastic money. Despite all, one-man small businesses should also use a cheque to withdraw money from their current bank accounts like macro businesses; it still makes a lot of real sense when they use bank cards just for a start for their cash withdrawals. Given these, all entrepreneurial efforts taken when establishing these businesses to avoid legal and financial risks, there is still much out there to look out for to protect their cash when effecting deposits and withdrawals using black stripped bank cards. These entrepreneurial management strategies are tailor-made to help small firms which have just been established as they have not as yet fully established themselves evade ID theft. In order to achieve to avert this kind of crime, this paper will then provide few tips as management strategies befitting small firms to rather circumvent the said crime. This paper also takes cognisance of what the South African (SA) law actually says to protect small firms from this particular financial risk brought about by this crime.
Academic geriatric programs in US allopathic and osteopathic medical schools.

CONTEXT By 2030, 20% of the US population will be older than 65 years compared with 12.4% in 2000. The development of geriatric medicine research and training programs to prepare for this increasing number of older individuals is largely dependent on the successful establishment of academic geriatric medicine programs in medical schools. OBJECTIVE To assess the structure, resources, and activities of academic geriatric medicine programs in US allopathic and osteopathic schools of medicine. DESIGN, SETTING, AND PARTICIPANTS Survey distributed to the academic geriatric medicine leaders of the 144 US allopathic and osteopathic medical schools in March 2001. MAIN OUTCOME MEASURES Organizational structure, program information, curriculum, budgetary issues, and characteristics of academic geriatric medicine leaders. RESULTS A total of 121 program directors (84%) responded. Most schools (87%) had an identifiable academic geriatric program structure, with 67% established after 1984. The greatest proportion of faculty and staff time (40%) was spent in clinical practice, followed by research and scholarship (12%), residency and fellowship education (10% each), and medical student education (7.8%). Clinical practice accounted for the greatest portion (27%) of revenue, and 25.7% of the programs had total annual budgets of less than $250,000, while 11% had budgets of greater than $5 million. The largest obstacles to achieving the goals of an academic geriatric medicine program were a lack of research faculty and fellows and poor clinical reimbursement. CONCLUSIONS Most US medical schools have an identifiable academic geriatric medicine program; most have been established within the last 15 years. Resources are needed to train faculty for roles as teachers and researchers and to develop medical school geriatric programs of the size and scope equivalent to other academic disciplines.
ON THE IMPORTANCE OF CONSIDERING CHANNEL MICROFORMS IN GROUNDWATER MODELS OF HYPORHEIC EXCHANGE

The infiltration of stream water in the sediment and its return to the stream—a process known here as hyporheic exchange flows (HEF)—is a critical control of the structure and functions of the stream ecosystem. River restoration programmes will increasingly require quantitative methods for evaluating this influence. Previous studies have already shown the potential of numerical groundwater models to characterize HEF and compare restoration scenarios. Although various sources of uncertainty are acknowledged, the potential effect of small streambed structures (or microforms), such as grains or ripples, embedded in channel-unit scale structures (or macroforms), such as riffle-pool sequences, is commonly ignored. Here, a simple conceptualization through a 2-D vertical model is used to test whether (i) ignoring microforms in groundwater models at the macroform scale can impact estimations of residence times; (ii) microforms can influence HEF patterns driven by macroforms; and conversely (iii) the uncertainty of head measurements in stream piezometers can affect our understanding of HEF patterns. Results show that (i) residence times and flux estimations can be strongly affected by the modeller’s choice to represent microform-induced HEF or not; (ii) the interaction of the microform and macroform scales can induce various subsurface flow patterns; and (iii) the perceived significance of microform-induced HEF is highly sensitive to the uncertainty of in-stream measurements of subsurface heads. Little is known about the relative efficiency of these microform and macroform scales, which are effectively influencing exchange at different depths and interacting with each other. Future studies that consider biogeochemical cycling or streambed ecology should be placed in this context. It is also necessary to find ways of including this source of uncertainty in groundwater models of HEF. key words: hyporheic; river restoration; riffle-pool; groundwater; pumping exchange; scale
Immunobiology of DC in NOD mice

NOD mice spontaneously develop diabetes between 15 and 20 weeks of age, which is preceded by insulitis characterized by the infiltration of lymphocytes. Dendritic cells (DC) are among the first cells to infiltrate the islet and they have been implicated in the pathogenesis of the disease. Our work has been concerned with the detailed characterization of four distinct DC populations in NOD mice: two derived from bone marrow (BM) cells cultured in either granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) plus interleukin‐4 (IL‐4) or GM‐CSF alone and two from the spleen of Flt3 ligand (Flt3L) ‐treated mice, isolated on the basis of CD8α expression. Phenotypic and functional differences between these DC subsets in NOD mice have been identified. In addition, we obtained a lower yield of NOD BM‐derived DC and they expressed higher levels of cell‐surface CD40 and IL‐12 p40 mRNA than BM‐derived DC from the diabetes‐resistant strain, B10.BR. We have also investigated the ability of these DC populations to modulate the development and progression of diabetes in NOD mice. J. Leukoc. Biol. 66: 276–280; 1999.
Cognitive complaints in women with fibromyalgia: Are they due to depression or to objective cognitive dysfunction?

ABSTRACT Introduction: Cognitive complaints are common in fibromyalgia, but it is unclear whether they represent an objective cognitive dysfunction or whether they could be explained by depressive symptoms. Here, we aim to elucidate the frequency of subjective cognitive complaints in a sample of women with fibromyalgia, in addition to analyzing associations between these subjective complaints and objective measures linked to the attention and executive cognitive domains. Finally, we aim to investigate the ability of demographic, clinical, and psychological variables to explain the subjective complaints observed. Method: One hundred and five women aged 30–55 years diagnosed with fibromyalgia completed a neuropsychological assessment, which included measures of attention and executive functions. They also completed self-report inventories of subjective cognitive complaints, depression, anxiety, intensity of pain, sleep quality, everyday physical functioning, and quality of life. Results: Eighty-four percent of the patients reported subjective cognitive complaints. Depression scores, everyday physical functioning, and working memory performance were most strongly associated with subjective cognitive complaints. These three variables were significant predictors for subjective cognitive complaints with a final model explaining 32% of the variance. Conclusions: Cognitive complaints are very frequent in patients with fibromyalgia, and these are related to functional and cognitive impairment as well as to depressive symptoms.
Stability Analysis of an Efficient Anti-Collision Protocol for RFID Tag Identification

A stable and efficient anti-collision protocol is very important for tags identification in many radio frequency identification (RFID) system such as flow productions and automatic controls. In this paper, we introduce an efficient anti-collision protocol named collision tree protocol (CT) and analyze the stability of it in detail. Both theoretical results and experimental results indicate that the performance of CT is only dependent on the number of tags to be identified and not influenced by the distribution of tag IDs and other factors, and the average performance of CT for one-tag identification converges to a constant. According to the definition of the stability of an anti-collision protocol, CT is a stable anti-collision protocol for RFID tag identification. The stability proposed in this paper provides a new performance metric for evaluating the performance of different anti-collision protocols for RFID tag identification. As a stable protocol, the time delay and the power consumption for one-tag identification of CT tend to be constant. Therefore, CT can be applied to various tags identification systems and especially suited to the RFID systems in which both the time delay and the power consumption for tags (or objects) identification are required to be limited and stable.
Data Transformation Methodologies between Heterogeneous Data Stores - A Comparative Study

With the advent of the NoSQL Data Stores, solutions for data migration from traditional relational databases to NoSQL databases is gaining more impetus in the recent times. This is also due to the fact that data generated in recent times are more heterogeneous in nature. In current available literatures and surveys we find that in-depth study has been already conducted for the tools and platform used in handling structured, unstructured and semi-structured data, however there are no guide which compares the methodologies of transforming and transferring data between these data stores. In this paper we present an extensive comparative study where we compare and evaluate data transformation methodologies between varied data sources as well as discuss the challenges and opportunities associated with it.
December Doldrums, Investor Distraction, and the Stock Market Reaction to Unscheduled News Events

We document that the stock market's reaction to unscheduled firm-specific news such as credit rating downgrades and 8-K filings is significantly weaker during December as compared to other months. In contrast, the market's reaction to scheduled earnings announcements is not significantly different in December. We find a similar pattern for trading volume. However, this December distraction does not affect firms with greater visibility, such as larger firms, firms with higher analyst following, or higher institutional ownership. Our results highlight how investor distraction during the December holiday season can lead to a muted market reaction to unscheduled, but salient, firm-specific news.
Uso de Bluetooth Smart e de aplicações móveis para melhorar a experiência de compra dentro de superfícies comerciais

Location systems have evolved over the years. Triangulation of GSM signals, Global Positioning System and other technologies had been developed in order to indicate accurately the location of the user. The problem with these kind of solutions is their ineffectiveness inside buildings. With the proliferation of smartphones, the indoor location has become even more important. To solve this problem, various technologies have been developed as the bluetooth beacons. This technology is a valid and cost-effective solution to solve this problem. In this dissertation is exposed the development of a system that takes advantage of bluetooth beacons in order to Enhanced Shopping Experience. For this purpose it was designed a system capable of accurately indicate the location of a particular product and generate and indicate the path that the user should follow in order to collect all the products present on user’s shopping list. This document shows the development of one of the applications of this system, responsible for estimate the location of the user within the building, with an average error of near 1 meter and is able to generate the shortest route with a average margin of error of 9.01% over the best route. With this dissertation can be concluded that it is possible to create a effective low cost system to guide a user inside a building.
Impact of new financial aid trend: attitudes and opinions

Rihab Ezzat Saadeddine IMPACT OF NEW FINANCIAL AID TREND: ATTITUDES AND OPINIONS 2008/09 Dr. Burton R. Sisco Master of Arts in Higher Education Administration The purpose of this study was to assess the attitudes of administrators towards the new financial aid trend that was initiated by some well-endowed private universities, and its impact on colleges and universities. The study also explored personal attitudes towards need and merit based aid, and recommendations to improve students' accessibility and affordability in higher education. Data analysis suggests that the majority of administrators have a positive attitude towards the general principle behind the new financial aid trend. However, they stated that this new trend is not broad and that it does not address the financial aid need of most students at other institutions. It was also reported that the new financial aid trend would not impact public universities, but would negatively impact small private universities. Furthermore, the study indicated that the majority of administrators agreed that eligibility for financial aid should be based on merit and need based aid, but with more emphasis on need aid. Administrators recommended that colleges and universities could increase students' accessibility and decrease educational costs, by having clear and defined policies; offering pre-college programs; diversifying their revenue streams; joining services; adhering to the university's mission; and decreasing operational costs. ACKNOWLEDGMENTS I would like to express my gratitude and thank all those who have helped and inspired me to complete this research. I especially want to thank my advisor, Prof. Burton Sisco, whom I am deeply indebted to for his guidance during my research. His support, enthusiasm, encouragement, and stimulating suggestions helped me tremendously to finish this paper. He was always accessible and willing to help me with my research. I would like to give my special thanks and deepest gratitude to my Husband Issam whose patient, love, and support enabled me to complete this work. To my husband Issam and son Iyad I dedicate this work.
Resource allocation in Eucalyptus

xvi CHAPTER 1 GENERAL INTRODUCTION . 1 1.1 OVERVIEW . 1 1.2 CURRENT KNOWLEDGE GAPS . 6 1.3 THESIS OBJECTIVES . 10 1.4 THESIS OUTLINE . 14 1.5 REPRODUCIBLE RESEARCH . 18 CHAPTER 2 BELOWGROUND SINK LIMITATION ALTERS GROWTH AND CARBON BALANCE OF EUCALYPTUS SEEDLINGS . 19 2.1 ABSTRACT . 19 2.2 INTRODUCTION . 20 2.3 MATERIALS AND METHODS . 25 2.4 RESULTS . 35 2.5 DISCUSSION . 52 iv 2.6 SUPPORTING INFORMATION . 61 CHAPTER 3 RAPID RESPONSE OF MESOPHYLL CONDUCTANCE TO LIGHT AVAILABILITY ALLOWS SHADE LEAVES TO TAKE ADVANTAGE OF SUNFLECKS . 64 3.1 ABSTRACT . 64 3.2 INTRODUCTION . 65 3.3 MATERIALS AND METHODS . 70 3.4 RESULTS . 81 3.5 DISCUSSION . 95 3.6 SUPPORTING INFORMATION . 101 CHAPTER 4 ELEVATED ATMOSPHERIC CO2 AND DROUGHT DOES NOT ALTER TOTAL BELOWGROUND CARBON ALLOCATION IN EUCALYPTUS SALIGNA . 107 4.1 ABSTRACT . 107 4.2 INTRODUCTION . 108 4.3 METHODS . 113 4.4 RESULTS . 125 4.5 DISCUSSION . 139 4.6 SUPPORTING INFORMATION . 146 CHAPTER 5 SYNTHESIS AND CONCLUSIONS . 149 5.1 SYNTHESIS . 149 v 5.2 CONCLUSIONS . 159 REFERENCES . 162
Heterogeneous Antibody Responses in Tuberculosis

ABSTRACT Antibody responses during tuberculosis were analyzed by an enzyme-linked immunosorbent assay with a panel of 10 protein antigens of Mycobacterium tuberculosis. It was shown that serum immunoglobulin G antibodies were produced against a variety of M. tuberculosis antigens and that the vast majority of sera from tuberculosis patients contained antibodies against one or more M. tuberculosis antigens. The number and the species of serologically reactive antigens varied greatly from individual to individual. In a given serum, the level of specific antibodies also varied with the antigen irrespective of the total number of antigens recognized by that particular serum. These findings indicate that person-to-person heterogeneity of antigen recognition, rather than recognition of particular antigens, is a key attribute of the antibody response in tuberculosis.