Miracle-dz/UniFAR-FTUs huggingface dataset:
Antitumor promoting activity of (−)‐epigallocatechin gallate, the main constituent of “Tannin” in green tea

(−)‐Epigallocatechin gallate (EGCG) is the main polyphenolic constituent of extracts of green tea. EGCG slightly inhibited specific binding of [3H] 12‐0‐tetradecanoylphorbol‐13‐acetate ([3H]TPA) to a particulate fraction of mouse skin, and caused a prompt decrease in the phorbol ester receptor number in mouse skin. It also inhibited the activation of protein kinase C by teleocidin. In week 25 of a two‐stage carcinogenesis experiment in mouse skin, tumors were found in 53% of the mice treated with 7,12‐dimethylbenz(a)anthracene (DMBA) plus teleocidin, but in only 13% of those treated with DMBA plus teleocidin and EGCG. The average number of tumors per mouse in these groups was 2.1 and 0.1, respectively. No tumors were found in other groups treated with DMBA alone or teleocidin and EGCG alone. Thus EGCG inhibited tumor promotion by teleocidin.
Testing device for a complex measurement of the performances nozzles

Testing device for a complex measurement of the performances nozzles Jozef Sawa1); Bruno Huyghebaert2; Stanislaw Parafiniuk1) 1 University of Life Sciences, Lublin, Poland, Department of Machinery Exploitation and Management in Agricultural Engineering, e-mail: jozef.sawa@up.lublin. pl 2 Walloon Agricultural Research Centre – Gembloux, Belgium, e-mail: huyghebaert@cra.wallonie. be DOI 10.5073/jka.2012. 439.027
The Effect of Formaldehyde and Blood Treatments of Sunflower Meal on the Degradation Characteristics and Effective Degradability in Rumen

This study was carried out to evaluate the effects of formaldehyde and blood treatments of sunflower meal on the degra-dation characteristics and effective degradability of dry matter(DM), organic matter and crude protein. Sunflower meal treated with 0.8 g formaldehyde/100 g crude protein, 0.5, 0.75, 1.5 and 2 L whole blood/kg DM. Three adult rumen fistulated merino rams were used in this study. Samples were incubated in the rumen for 2, 4, 8, 16, 24, 48 and 60 h. Formaldehyde treatment of sunflower meal reduced the amount of rapidly soluble protein, degradation rate constant and effective degradability of protein by 75.36, 22.70 and 20.89%, respectively. However the protein which in time will degrade increased by 19.57% and maximum potential degradability of protein in rumen was not affected. Treating sunflower meal with 0.5, 0.75 and 1.5 L blood/kg DM did not affect degradability after 48 h incubation and effective degradability in rumen. Blood treatment at the level of 2 L/kg DM reduced the degradation rate consant and effective degradability of protein in rumen by 39.40 and 24.84%, respectively.
The Role of Ca2+/Calmodulin Dependent Protein Kinase II Alpha in Group 1 Metabotropic Glutamate Receptor Endocytosis and Signalling

Group 1 metabotropic glutamate receptors (mGluR1 and mGluR5) are G-protein coupled receptors (GPCRs) activated by glutamate. mGluR1/5 couples to Gαq/11 and releases Ca2+ from the endoplasmic reticulum. Ca2+/calmodulin-dependent protein kinase II alpha (CaMKIIα) can be activated by Gαq/11-mediated Ca2+ release through binding of Ca2+/calmodulin. Results from a proteomic screen identified CaMKII as a novel mGluR-interacting protein. Therefore, we hypothesized that CaMKIIα associates with group 1 mGluRs and this association alters mGluR1/5 signalling and internalization. Firstly, we demonstrated the novel association between CaMKIIα and mGluR1/5 by co-immunoprecipitation of transiently transfected proteins in HEK293 cells and of endogenous proteins in mouse hippocampal tissue. Next, we showed that the second intracellular loop of the mGluR1a receptor is sufficient for this association. Furthermore, CaMKIIα significantly enhances agonist-induced internalization of group 1 mGluRs. Yet, it does not appear that CaMKIIα plays a significant role in receptor signalling by either ERK1/2 phosphorylation or inositol phosphosphate formation. Both CaMKIIα and mGluR1/5 play an important role in memory, learning and synaptic transmission. Understanding how these two players work together could provide a mechanism for reducing excitotoxicity through desensitization of mGluR1/5 by CaMKIIα.
Assessment of GPR30, a Seven Transmembrane-Spanning Estrogen Receptor, as an Oncogene

Abstract : Our prior work has linked the seven transmembrane estrogen receptor GPR30 to specific estrogen binding (Thomas et al, 2005), rapid estrogen action (Filardo and Thomas, 2005) and the development of metastatic breast cancer in man (Filardo et al, 2006). To further address the role of GPR30 in experimental breast cancer biology, transgenic mice were created for the purpose of overexpressing wild type or active GPR30 in the mammary gland using the mouse mammary tumor virus tissue specific promoter. Two suitable candidate founder mice with stably integrated wild-type GPR30 were generated (T6- 1A and T6-2E). We were unsuccessful in our attempts to generate an active GPR30 allele as assessed by in vitro assays outline in this proposal during this time frame. A no cost extension was requested to further propagate the wild-type mice and to analyze their capacity to develop mammary adenocarcinoma.
Antocianinas, polifenoles, actividad anti-oxidante de sachapapa morada (Dioscorea trifida L.) y evaluación de lipoperoxidación en suero humano

The total content of anthocyanins, total polyphenols were determined spectrometrically. The anti-oxidant activity of the Dioscorea trifida L extracted pigments was evaluated using • • + different anti-oxidant assays including: free radical scavenging (DPPH , and ABTS ), as well as superoxide anion radical scavenging. The in vitro anti-oxidant activity of Dioscorea trifida L extracts against the lipidic peroxidation in human serum was evaluated at different concentrations. The largest inhibition percentage (66,37%) was achieved at the extract concentration of Dioscorea trifida L of 2,06 mg/mL. The inhibition coefficient to 50% (IC ) was 1,824 mg/mL. 50 The pigments extracted from purple sachapapa are natural colorants that exhibit excellent anti-oxidant activity and offer the potential to be beneficial for health and that could be applied in the food and health science fields.
Tiling resolution array CGH of dic(7;9)(p11∼13;p11∼13) in B-cell precursor acute lymphoblastic leukemia reveals clustered breakpoints at 7p11.2∼12.1 and 9p13.1

The dic(7;9)(p11∼13;p11∼13) is a recurrent chromosomal abnormality in acute lymphoblastic leukemia (ALL), mainly of B-lineage. Although more than 20 dic(7;9)-positive ALLs have been reported to date, the molecular genetic consequences of this aberration are unknown. We performed tiling resolution (32K) genome-wide array-based comparative genomic hybridization (array CGH) analysis of three cases with dic(7;9) in order to characterize the breakpoints on 7p and 9p. The analysis showed a clustering of breakpoints within 9p13.1 in all three cases and within 7p11.2 in two cases; the array CGH revealed two different breakpoints – 7p12.1 and 7p14.1 – in the remaining case. Based on these findings the abnormality should hence be designated dic(7;9)(p11.2∼12.1;p13.1). Locus-specific fluorescence in situhybridization analysis of one of the cases narrowed down the 7p11.2 breakpoint to a <500-kb segment in this sub-band, a region containing three known genes. Unfortunately, lack of material precluded further molecular genetic studies, and it thus remains unknown whether the pathogenetically important outcome of the dic(7;9) is formation of a chimeric gene or loss of 7p and/or 9p material.
Régulations post-transcriptionnelles de l'expression génique de la cellule hôte en réponse à l'infection bactérienne

Listeria monocytogenes est un pathogene frequent du betail et l’agent de la listeriose humaine, une maladie grave pour les femmes enceintes, les personnes âgees ou immunodeprimees. La proliferation de cette bacterie dans les cellules qu’elle infecte s’accompagne d’une reprogrammation de l’expression genique et des fonctions cellulaires. Apres un doctorat sous la direction de Micheline Fromont-Racine (groupe d’Alain Jacquier) a l’Institut Pasteur sur la biogenese des ribosomes, mes travaux de post-doctorat dans le groupe dirige par Bertrand Seraphin au Centre de Genetique Moleculaire (Gif-sur-Yvette) ont mis en evidence une nouvelle activite enzymatique – une endonuclease – dans l’un des complexes de degradation des ARN eucaryotes les plus etudies, l’exosome. Recrutee a l’INRA en tant que chargee de recherche en 2008, j’ai rejoint jusqu’en 2014 l’equipe dirigee par Pascale Cossart a l’Institut Pasteur. J’y ai etudie comment la bacterie pathogene Listeria monocytogenes detourne la machinerie de repression chromatinienne des cellules qu’elle infecte, pour moduler certaines reponses immunitaires. Je dirige depuis octobre 2014 a l’Institut de Biologie de l’Ecole Normale Superieure (IBENS) une equipe de recherche qui etudie les mecanismes post-transcriptionnels regulant les ARNm eucaryotes en reponse a l’infection par Listeria monocytogenes. Nous travaillons sur quatre problematiques principales : L’infection conduit-elle a une degradation globale ou specifique des ARNm ? La machinerie et l’efficacite de la traduction sont-elles perturbees ? Quels effecteurs moleculaires de la bacterie ou de l’hote sont impliques dans ces processus ? Quelles en sont les consequences patho-physiologiques ? Ces travaux, reposant sur des technologies haut debit adaptees a l’etude du devenir des ARNm, ainsi que sur des approches plus classiques de microbiologie cellulaire, permettront de mieux comprendre les imperatifs de l’adaptation mutuelle entre la bacterie et son hote, d’identifier de nouveaux facteurs de virulence, et d’explorer les voies de controle traductionnel eucaryotes dans un contexte d’infection.
THE EFFECT OF VISION AND ROLE CLARITY ON TEAM PERFORMANCE DOI: 10.17261/Pressacademia.2015313067

A strong vision and role definition can provide direction to a team and can positively impact its ability to succeed. However, although many studies conclude that vision and role clarity are important at the organizational level, the impacts of vision and role clarity on innovation/teams have received far less attention. The purpose of this research is to discuss vision components and Role Clarity, and explore their impacts on team performance. After studying the vision on a series of 9 innovation teams at three companies (Apple, IBM, and HP), we empirically tested the impact of the two components of vision (Vision Clarity, and Vision Support) and Role Clarity on overall team performance. Data were collected from 75 team members. We found that Vision Clarity has a positive effect on team performance. We also found that Vision Support and Role Clarity are not significantly related to team performance.
Snapping shrimps of the genus Alpheus Fabricius, 1798 from Brazil (Caridea: Alpheidae): updated checklist and key for identification

We provide an updated checklist of the snapping shrimps of the genus Alpheus Fabricius, 1798 (Crustacea: Alpheidae) from the Brazilian coast, as well as a key for their identification. The checklist was based on an exhaustive analysis of the literature published up to now, supported by analysis of material of 24 of the 33 species presently recorded from Brazil. Illustrations of the main diagnostic characters are provided to facilitate the identification process, as well as color photographs of 16 species collected by the authors, to facilitate the identification of fresh specimens. For each species, we provide information on the distribution and a list of previous records for the Brazilian coast. Comments on dubious records or taxonomic remarks are provided when appropriate. Finally, the zoogeography of the species is briefly discussed.
Population Dynamics of the Australian Plague Locust, Chortoicetes Terminifera (Walker), in Central Western New South Wales. I. Reproduction and Migration in Relation to Weather.

On three adjacent experimental plots in central western New South Wales, from 1971 to 1974, population fluctuations of the Australian plague locust were interpreted in terms of the interactions between reproductive rates (Ro) between generations and net migration losses or gains (M plus or minus ) within generations. Changes due to variations in net migration fluctuated over a greater range than those due to variations in reproductive rates, and were the major cause of the population fluctuations during the 16 generations studied. Variations in rainfall during breeding accounted for nearly 90% of the variance in reproductive rates, provided rain followed relatively dry periods. Changes caused by migration resulted from episodic, often long-range and generally southward displacements of adults at night in the gradient wind during disturbed weather. The frequency and intensity of migration depended primarily on the availability of potential emigrants and secondarily on the incidence of weather favourable for takeoff and prolonged flight. The incidence of rainfall before periods of migration was higher than expected. Adults thus migrated into areas already favourable for successful breeding. Long-term median rainfall during breeding at Trangie gave mean reproductive rates greater than 1 per generation. Hence, population surpluses tend to be produced and generally disperse south to areas less favourable for breeding. There was close correspondence between the occurrence of heavy drought-breaking rains, leading to substantial population increases and migrations, and the observed incidence of outbreaks in this area. Changes in the relative importance of reproduction and migration in the drier breeding areas further inland are discussed in relation to historical changes in the distribution and abundance of the plague locust.
Modelling monthly NH3 emissions from dairy in 12 Ecoregions of Canada

Sheppard, S. C. , Bittman, S. , Swift, M. L. and Tait, J. 2011. Modelling monthly NH 3 emissions from dairy in 12 Ecoregions of Canada. Can. J. Anim. Sci. 91: 649–661. Ammonia (NH3) from livestock manure is emitted from barns, storages and manured land, and is a loss to the farm operations, while atmospheric NH3 has potential impacts beyond the farm, including human health and ecological damage. Models are used to estimate the intensity and spatial extent of NH3 emissions, and this paper reports a recent model developed for quantifying emissions from the dairy sector in Canada. The estimated overall average emission to the atmosphere in Canada in 2006 was 42.4±9.0 kg NH3 cow−1 yr−1 from a lactating cow, and total emission from the Canadian dairy sector was 56000 t NH3. On many farms the NH3 emissions may have been a significant portion of the N requirements of their crops. The emission estimates in the 12 Ecoregions were proportional to the animal census. Emissions generally peaked in May, mainly because of.
Daphnis nerii (Lepidoptera: Sphingidae), a New Pest of Oleander on Guam, Including Notes on Plant Hosts and Egg Parasitism

Daphnis nerii (L.), the oleander hawk moth, was first detected on Guam in August, 2005. In the field, larvae were observed feeding only on oleander, Nerium oleander L. However, in laboratory feeding tests, larvae survived to adulthood equally well on N. oleander and Ochrosia mariannensis A. DC. , indicating that some endemic plants may be impacted by this invasive insect. Thirty out of thirty two D. nerii eggs collected from oleander leaves were parasitized. These eggs produced 181 hymenopter- ous parasitoids belonging to four species: Trichogramma chilonis Ishii (Trichogram- matidae), Eupelmus sp. (Eupelmidae), Telenomus (Aholcus) sp. (Scelionidae), and Ooencyrtus nr. papilionis (Encyrtidae).
Capricious bioinvasions versus uncoordinated management strategies: how the most unlikely invaders can prosper under the current UK legislation framework

Bioinvasions are known to be highly unpredictable and context-dependent, varying both spatially and temporally, particularly in areas of intense anthropogenic activity and disturbance. Even the most unlikely invader can rapidly become problematic in the absence of frequent, coherent, and flexible management strategies. Using the recent spread of the Chilean oyster (Ostrea chilensis Philippi 1845) within a designated Special Area of Conservation, the paper describes what can happen to seemingly innocuous non-native species under the often complicated and uncoordinated current UK legislation framework. Following >30 years of containment at Tal y Foel (North Wales, UK), O. chilensis, a species with a highly-reduced natural dispersal capacity, has now spread over a range of >30 km of shoreline. Alternative vectors, including rafting and several anthropogenic activities, are likely to have facilitated the dispersal of O. chilensis away from Tal y Foel. Areas of high oyster densities (maximum = 232 oysters m-2) have become established both close to and distant from the site of original introduction. The presence of all year classes throughout the observed age range (≤7 and ≤9 years old in the intertidal and subtidal populations, respectively) confirms regular annual recruitment in the area. Information is now urgently required regarding the factors that promote the persistence and spread of O. chilensis within its new environment, as well as the impacts of its increasing localized dominance on the native biodiversity and ecosystem function. As well as providing valuable, up-to-date information on the recent spread of this non-native species, the paper highlights discrepancies in the current UK legislation framework that allow for the successful establishment and spread of even the most unlikely invaders. the formation of a comprehensive and dedicated EU legal framework for managing invasives is advocated that also promotes coherence and continuity with impending legislative instruments concerning other relevant sectors. Copyright © 2011 John Wiley & Sons, Ltd.
Effects of Menstrual Cycle and Room Temperature on Color Preference

This study investigated the effects of menstrual cycle on color preference in nine normally menstruating female subjects. They were instructed to choose their preferred color out of 45 Munsell hues every 5 min at ambient temperatures (T a) of 28°C (630-800 h), from 28°C to 23°C (800-900 h) and at 23°C (900-930 h). Warmer color hues were preferred during the luteal phase than the follicular phase at 28°C, while there did not exist any significant differences at other T as. The findings that a preference for warmer colors occurred in the luteal phase at 28°C is discussed in terms of the load error between actual core temperature and its setpoint.
Locating a Small Cluster Privately

We present a new algorithm for locating a small cluster of points with differential privacy [Dwork, McSherry, Nissim, and Smith, 2006]. Our algorithm has implications to private data exploration, clustering, and removal of outliers. Furthermore, we use it to significantly relax the requirements of the sample and aggregate technique [Nissim, Raskhodnikova, and Smith, 2007], which allows compiling of "off the shelf" (non-private) analyses into analyses that preserve differential privacy.
Identification of Receptors for Prothymosin α on Human Lymphocytes

Abstract Prothymosin α (ProTα ) is a highly conserved and widely distributed protein whose physiological functions remain elusive. In previous work we identified high and low affinitybinding sites for ProTα in lymphoid cells. Here we demonstrate, by affinity crosslinking and affinity chromatography, the existence of three binding partners (31, 29, and 19kDa) for ProTα in the membrane of PHAactivated lymphoblasts. These surface molecules possess the expected affinity and specificity for a ProTα receptor. Examination of the expression of this complex of molecules by flow cytometry reveals that they bind ProTα in a specific and saturable way. In addition, the distribution of the receptor on the cell surface was studied by fluorescence microscopy; a caplike structure at one of the poles of the cells was identified. These results represent a new and promising approach in the research on ProTα, opening the way toward the understanding of the molecular mechanism of action of this protein.
Rapid Determination of Ginsenosides Rb 1 , Rf, and Rg 1 in Korean Ginseng Using HPLC

A simple gradient HPLC method for rapid determination of major ginsenosides (Rg₁ and Rb₁) and unique ginsenoside (Rf) of Korean ginseng (Panax ginseng C.A. Meyer) was developed. Within 50 min, three ginsenosides have been separated and identified on μ-Bondapak C 18 column (3.9×300 ㎜, 10 ㎛) with gradient elution using water and acetonitrile as a mobile phase. The method was validated in terms of linearity, accuracy, and precision. The correlation coefficients (r²) for calibration curves of ginsenosides were over 0.9997. The developed HPLC method was successfully applied to the analysis of ginseng samples and the recoveries of ginsenosides were in the range of 101.1~115% with RSD＜3.2%. The developed method could be used for rapid evaluation of the ginsenosides Rg₁, Rb₁, and Rf.
Forage yield and quality response of annual ryegrass (Lolium multiflorum) to different water and nitrogen levels

Water and nitrogen shortages in annual ryegrass (Lolium multiflorum) production can be improved by using proper irrigation and nitrogen scheduling methods. The aim of this research was to analyse the effect of water and nitrogen application on dry matter production and forage quality of annual ryegrass. Treatments consisted of three irrigation levels, scheduling application to field capacity once every two weeks (W1), once a week (W2) or twice a week (W3), and three nitrogen (N) levels, top-dressing after each harvest at a rate of 0 (N1), 30 (N2) or 60 (N3) kg N h−1 cycle−1. Yield increased as a function of the amount of water and nitrogen fertiliser applied. The dry matter (DM) content, in vitro organic matter digestibility (IVOMD), crude protein (CP) and metabolisable energy (ME) were higher in the treatments being irrigated once every two weeks. These results conclude that higher irrigation coupled with high N application significantly improved the dry matter yield, while water stress, to some extent, did improve the quality of the pasture by increasing the DM content, IVOMD, CP and ME values.
Validation of a forest values typology for use in national forest planning

Public values for national forestlands are assumed to underlie preferences for actual forest use and define the context for forest planning and decision making, but the relationship between preferences and attitudes toward forest management activities and public forest values (both use and nonuse values) is not well understood. Using data from a survey of Alaskan residents in the Chugach National Forest plan revision process, the relationship between attitudes toward forest management actions and forest values is examined using a variety of statistical procedures. Key findings indicate that: (1) survey respondents were able to identify with 13 distinct forest values based on a modified forest value typology developed by Rolston and Coufal (1991), (2) no obvious latent structure of variables or constructs emerged from factor analysis of the 13 forest values indicating that the forest value typology may not be easily simplified without compromising the exclusiveness of measured forest values, (3) small, but statistically significant correlations were found between attitudes toward specific forest management practices such as logging and mining and held forest values, and (4) forest values are modestly predictive of respondent preferences for specific forest planning decisions.