Miracle-dz/UniFAR-FTUs huggingface dataset:
Signal-Specific Activation and Regulation of Human Neutrophil Fcγ Receptors1

FcγRs with the ITIM domain have been shown to regulate the inflammatory signal delivered by the ITAM-containing FcγRs. In this study, we demonstrate that the function of human neutrophil FcγR type IIA (CD32A) is regulated in a distinct manner by different cell activation signals at the ligand-binding stage. Activation of neutrophils with fMLP up-regulated the ligand-binding function of CD32A, whereas PMA-mediated activation completely abolished ligand binding without altering CD32A expression. Furthermore, PMA treatment also abolished CD16B-dependent ligand binding irrespective of the level of expression. The effect of PMA was cell type specific, because the ligand-binding function of CD32A expressed on cultured cells such as K562 and CHO-CD32A transfectants was not affected by PMA. Interestingly, phorbol 12,13-dibutyrate, another phorbol ester, and IL-8 up-regulated CD32A-dependent ligand-binding function. These results demonstrate that regulation of CD32A-dependent ligand binding in human neutrophils is not only cell type specific but also activation signal specific. Moreover, these results suggest the possibility that signals delivered to neutrophils by various inflammatory stimuli can exert opposing effects on the function of human FcγRs, representing a novel inside-out regulatory mechanism of FcγR ligand binding.
Near-infrared emission lines trace the state-independent accretion disc wind of the black hole transient MAXI J1820+070

The black hole transient MAXI J1820+070 displayed optical P Cyg profiles and other wind-related emission line features during the hard state of its discovery outburst. We present near-infrared (NIR) spectroscopy covering the different accretion states of the system during this event. Our eight-epoch data set (VLT/X-shooter) reveals strong variability in the properties of the NIR emission lines. This includes absorption troughs and extended emission line wings with kinetic properties that are remarkably similar to those inferred from the wind signatures observed in optical emission lines, indicating that they most likely trace the same accretion disc wind. Unlike the optical features, these NIR signatures are not exclusive of the hard state, as they are also witnessed across the soft state with similar observational properties. This supports the presence of a relatively steady outflow during the entire outburst of the system, and it represents the first detection of an accretion disc wind in a black hole soft state at energies other than X-rays. We discuss the visibility of the wind as a function of the spectral band and the potential of NIR spectroscopy for wind studies, in particular during luminous accretion phases.
3D dynamics of the Orion cloud complex

We present the first study of the 3D dynamics of the gas in the entire Southern Orion cloud complex. We used the YSO's proper motions from Gaia as a proxy for the gas proper motion, together with gas radial velocities from archival CO data, to compute the space motion of the different star-forming clouds in the complex, including sub-regions in Orion A, Orion B, and two outlying cometary clouds. From the analysis of the cloud's orbits in space and time we find that they were closest about 6 Myr ago and are moving radially away from roughly the same region in space. This coherent 100-pc scale radial motion supports a scenario where the entire complex is reacting to a major feedback event that we name the Orion-6 event. This event, that we tentatively associate with the recently discovered Orion X population, shaped the distribution and kinematics of the gas we observe today, although it is unlikely to have been the sole major feedback event in the region. We argue that the dynamics of most of the YSOs carry the memory of the feedback-driven star formation history in Orion and that the majority of the young stars in this complex are a product of large-scale triggering, that can raise the star formation rate by at least an order of magnitude, as in the case of the Orion A's Head (the Integral Shape Filament). Our results imply that at the genesis of the Orion Nebula Cluster (and NGC2023/2024 in Orion B) lies a feedback/compression/triggering process.
Biochemical Characteristics of Species of Penicillium Responsible for the Rot of Citrus Fruits

Decaying oranges or other citrus fruits are almost invariably infected by one of two moulds, viz., P. digitatum Saccardo (= P. olivaceum Wehmer), which causes the olive-coloured rot, and P. italicum Wehmer, which is responsible for the blue-green rot. It has been found in the course of work that each of these species has very definite biochemical characteristics which are described in this paper.
Bioinformatics Study of the Sequence Analysis and Secondary Structure Prediction of Avicennia marina glyoxalase I protein

Glyoxalase 1 detoxifies α-oxoaldehydes, particularly methylglyoxal (MG) via different enzymatic and non-enzymatic reactions, a cytotoxic compound increased rapidly under stress conditions. The physiological significance of the glyoxalase system has not been clearly defined in plants. We used bioinformatics tools in this study; we performed sequence analysis; major part of Avicennia marina glyoxalase I proteins polypeptide consists of hydrophobic residues; comparison of salt tolerant glyoxalase I from Avicennia marina with that of salt sensitive plants multiple sequence alignment
ANTIBACTERIAL ACTIVITY OF ETHANOL EXTRACT OF MUSA PARADISIACA CV. PUTTABALE AND MUSA ACUMINATE CV. GRAND NAINE

Banana is one of the most popular fruits distributed all over the world. Traditional, the fruit, Stem juice, flowers of banana plants were used for treating diarrhoea (unripe), dysentery, menorrhagia, diabetes, antilithic, antiulcerogenic, hypoglycemic, hypolipidemic , antioxidant actions and inflammation, pain & snakebite. In order to justify the ethnomedicinal claims. The corm ethanol extracts of Musa acuminate cv. Grand naine and Musa paradisiaca cv. Puttabale were screened for potential antibacterial activity using agar well diffusion method against 8 clinical strains. Extracts of M. paradisiaca showed a significant level of bacterial inhibition against Proteus vulgaris (19.78±0.40), Pseudomonas aeruginosa (19.67±0.41) and Staphylococcus aureus (17.44±0.50), moderate activity against Salmonella typhi (16.89±0.48), Salmonella paratyphi (16.67±0.37), Klebsiella pneumoniae (15.56±0.50), Bacillus subtilis (15.11±0.42) and very less against Escherichia coli (09.11±0.39). The extract of M. acuminate showed a significant level of bacterial inhibition against Proteus vulgaris (18.89±0.42), P. aeruginosa (13.44±0.47), Staphylococcus aureus (15.56±0.38), moderate activity against Salmonella typhi (15.67±0.44), Salmonella paratyphi (20.44±0.56), Klebsiella pneumoniae (14.33±0.33), Bacillus subtilis (14.00±0.41) and the very less against Escherichia coli (12.44±0.53).
Differences between sheep and cattle in digestibility, voluntary intake and retention time in the rumen of three tropical grasses

In the assessment of the nutritive value of feeds for animals, measurement of digestibility has been a basic index since 1860 (Schneider & Flatt, 1965). In this laboratory, measurements of digestibility and voluntary feed consumption using sheep have been the basis of feed evaluation procedures for over 20 years (e.g. Milford, 1960). The plants with which our group is concerned, however, are mainly grazed by cattle, and it is important to establish the degree to which extrapolation of results from sheep to cattle is justified for tropical pasture species. Many comparisons between sheep and cattle in terms of digestibility of dry matter and other aspects of feed utilization have been made using temperate pasture species hays, silages, grains and mixed rations; this area has been thoroughly reviewed by Schneider & Flatt (1965). Very few such studies have been made using tropical species, and most of those in Australia have involved the native species Heteropogon contortus (Siebert & Kennedy, 1972) or mixed H. contortus and Bothriochloa decipiens (Playne, 1978), providing material of low quality, containing less than 1% nitrogen. Playne (1970) used one introduced tropical species, buffel grass (Cenchrus ciliaris), and in that work only one of three harvests contained over 1% nitrogen. In the present work three introduced tropical species have been examined, covering a wider range of quality than has been reported previously.
Screening and Functional Pathway Analysis of Pulmonary Genes Associated with Suppression of Allergic Airway Inflammation by Adipose Stem Cell-Derived Extracellular Vesicles

Background Although mesenchymal stem cell- (MSC-) derived extracellular vesicles (EVs) are as effective as MSCs in the suppression of allergic airway inflammation, few studies have explored the molecular mechanisms of MSC-derived EVs in allergic airway diseases. The objective of this study was to evaluate differentially expressed genes (DEGs) in the lung associated with the suppression of allergic airway inflammation using adipose stem cell- (ASC-) derived EVs. Methods C57BL/6 mice were sensitized to ovalbumin (OVA) by intraperitoneal injection and challenged intranasally with OVA. To evaluate the effect of ASC-derived EVs on allergic airway inflammation, 10 μg/50 μL of EVs were administered intranasally prior to OVA challenge. Lung tissues were removed and DEGs were compared pairwise among the three groups. DEG profiles and hierarchical clustering of the identified genes were analyzed to evaluate changes in gene expression. Real-time PCR was performed to determine the expression levels of genes upregulated after treatment with ASC-derived EVs. Enrichment analysis based on the Gene Ontology (GO) database and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were also performed to further identify the function of DEGs. Results Expression of paraoxonase 1 (PON1), brain-expressed X-linked 2 (Bex2), insulin-like growth factor binding protein 6 (Igfbp6), formyl peptide receptor 1 (Fpr1), and secretoglobin family 1C member 1 (Scgb1c1) was significantly increased in asthmatic mice following treatment with ASC-derived EVs. GO enrichment and KEGG pathway analysis showed that these genes were strongly associated with immune system processes and their regulation, cellular processes, single-organism processes, and biological regulation. Conclusion These results suggest that the DEGs identified in this study (PON1, Bex2, Igfbp6, Fpr1, and Scgb1c1) may be involved in the amelioration of allergic airway inflammation by ASC-derived EVs.
The creation of embryos for research and the protection of embryonic life - A view from the

Commentators in the rest of Europe often have difficulty in understanding why the United Kingdom has a very liberal regulation of embryo research and often misinterpret it as based on the view that embryos are not morally important. In the present paper I will try to explain how the UK reached its present regulatory position, what the likely future developments are and what it implies about the status of the embryo.
Ants show the way Down Under: invertebrates as bioindicators in land management

The sight of land managers poring over ant checklists is a regular occurrence in Australia, where ant monitoring has been successfully applied to a wide range of land-use situations. The robustness of ants as ecological indicators has been consistently demonstrated, and is supported by an extensive understanding of their community dynamics in relation to disturbance. Despite the widespread recognition of the value of terrestrial invertebrates as bioindicators, the use of ants represents one of the few examples where invertebrates are widely adopted in land management as indicator organisms, as opposed to being consigned to the “too hard” basket. The use of invertebrates as bioindicators in land management will always require specialist expertise and a substantial investment in resources. However, research in Australia shows that invertebrate monitoring does not require comprehensive surveys, and that it is possible to simplify sampling and processing without compromising indicator performance. This should.
Totally Implanted Port May Be an Alternative to Centrally Inserted Central Catheter for Measurement of Central Venous Pressure

Background A conventional centrally inserted central catheter (CICC) is frequently used to measure central venous pressure (CVP) to monitor the cardiocirculatory status of patients. The tip of the totally implanted port (TIP) is inserted at the same location in the superior vena cava as that of a CICC, and the TIP has been implanted in many patients with cancer. Measurements of CVP using CICC (CICCP) and TIP (TIPP) may be closely related. Material and Methods. Ten patients with TIPs in an intensive care unit were prospectively studied, and 121 records of 4536 paired CICCP and TIPP measurements were collected. A bench test in a static or dynamic setting was performed, and 598 paired measurements taken using CICC and TIP were recorded. Results The measurement of TIPP was highly correlated with that of CICCP in patients with cancer, especially those in a calm state. Patients with a calm state and ≥3 consecutive identical TIPP were recorded (≥30 seconds), and 90% of the mean difference between CICCP and TIPP was ≤2 mmHg. The pressure measurements recorded using CICC and TIP were identical in both the static and dynamic bench tests. Conclusions TIP may be an alternative to CICC for measuring CVP.
Changes in Photosynthesis and Carbohydrate Metabolism in Mature Apple Leaves in Response to Whole Plant Source-Sink Manipulation

Photosynthesis and carbohydrate metabolism in apple (Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf. ) source leaves were monitored during a 7-day period after source-sink manipulations by girdling or partial defoliation treatments. In the girdling treatment, sorbitol, sucrose, glucose, and starch accumulated in leaves, and net photosynthetic rates (Pn) at 350 µL·L -1 CO2 decreased during a 7-day period. Pn measured at 1000 µL·L -1 (CO2) was also decreased but the changes were less. Stomatal conductance and intracellular CO2 concentration decreased markedly in leaves of girdled shoots. When shoots were partially defoliated, starch and glucose concentrations in remaining source leaves declined steadily during the 7-day study period. Sorbitol and sucrose concentrations decreased during the first 2 days after defoliation, then increased the following 5 days. Pn of the remaining leaves measured at ambient and elevated CO2 levels were enhanced markedly. Aldose-6-phosphate reductase activity in source leaves increased markedly from 27.5 to 39.2 µmol·h -1 ·g -1 fresh weight (FW) after partial defoliation but remained unchanged in leaves after girdling. Selective and maximum sucrose phosphate synthase (SPS) activities increased following partial defoliation and decreased following girdling. ADP-glucose pyrophosphorylase activity remained relatively unchanged in the partial defoliation treatments but increased markedly in the girdled-shoot leaves. These results suggested that girdling-induced photosyn- thetic inhibition is mainly due to stomatal limitation, however, the photosynthesis enhancement by partial defoliation may be due primarily to acceleration of photosynthetic capacity per se. These studies showed that the metabolism of sorbitol, sucrose and starch, three photosynthetic end products in mature apple leaves, was coordinately regulated in source leaves in response to source-sink manipulations.
Free and total cortisol levels in semelparous and iteroparous chinook salmon

Total cortisol, free cortisol and percent free cortisol were all significantly higher in semelparous male chinook salmon Oncorhynchus tshawytscha than in iteroparous and immature fish. The findings suggest that the regulation of both total and free cortisol concentrations may play key roles in mediating the post-spawning death of semelparous Pacific salmon.
Ozone action on survival and storage life of live and chilled tilapia

The effects of applying ozone treatment to live pond-raised tilapia on the survival of live fish and length of storage of chilled fish were evaluated according to sensory, chemical, physical, and bacteriological tests. Long-term low-level ozonation extended survival and improved the physical state of live fish in a tank and may be feasible for use in fish markets to improve commercial quality of live fish. Ozone treatment of tilapia while still alive prolonged their storage life as chilled tilapia by 12 days (40%) and improved their quality when stored at 0°C but had little effect at 5°C. The improvement probably resulted from an initial reduction and prevention of growth of spoilage bacteria such as Pseudomonas fluorescens, Shewanella putrefaciens, and Aeromonas sobria. At 0°C, P. fluorescens was most sensitive to ozone, while Brevundimonas diminuta and Pseudomonas putida were not. The combination of ozone pretreatment and storage at 0°C appears to be a feasible means of prolonging the storage life of chilled tilapia.
Home-based or remote exercise testing in chronic respiratory disease, during the COVID-19 pandemic and beyond: A rapid review

Objectives: To identify exercise tests that are suitable for home-based or remote administration in people with chronic lung disease. Methods: Rapid review of studies that reported home-based or remote administration of an exercise test in people with chronic lung disease, and studies reporting their clinimetric (measurement) properties. Results: 84 studies were included. Tests used at home were the 6-minute walk test (6MWT, two studies), sit-to-stand tests (STS, five studies), Timed Up and Go (TUG, 4 studies) and step tests (two studies). Exercise tests administered remotely were the 6MWT (two studies) and step test (one study). Compared to centre-based testing the 6MWT distance was similar when performed outdoors but shorter when performed at home (two studies). The STS, TUG and step tests were feasible, reliable (intra-class correlation coefficients >0.80), valid (concurrent and known groups validity) and moderately responsive to pulmonary rehabilitation (medium effect sizes). These tests elicited less desaturation than the 6MWT, and validated methods to prescribe exercise were not reported. Discussion: The STS, step and TUG tests can be performed at home, but do not accurately document desaturation with walking or allow exercise prescription. Patients at risk of desaturation should be prioritised for centre-based exercise testing when this is available.
Diastolic Blood Pressure Does Not Influence Cardiovascular Outcomes in Type 2 Diabetes; or Does It?

Hypertension and diabetes are prominent cardiovascular (CV) risk factors and major issues of public health (1,2). The common coexistence of hypertension and type 2 diabetes is long-established and represents a “deadly” combination: diabetes increases CV risk threefold at any level of systolic blood pressure (SBP), while the presence of hypertension in patients with diabetes increases CV risk by fourfold (3,4). The optimal level of blood pressure (BP) in patients with diabetes has been a matter of debate for many years (5–7). Following older observational evidence and interventional data from the UK Prospective Diabetes Study (UKPDS) 38 and Hypertension Optimal Treatment (HOT) study, which showed CV benefits with diastolic blood pressure (DBP) of 15% 10-year CV risk) with type 2 diabetes to a target SBP of <120 mmHg or <140 mmHg. After 4.7 years of follow-up, the two groups had no differences in the primary outcome (nonfatal myocardial infarction, nonfatal stroke, death from CV causes) (hazard ratio [HR] 0.88, 95% CI 0.73–1.06; P = 0.20), CV mortality, and all-cause mortality; those in the intensive group had fewer stroke events (HR 0.59, 95% CI 0.39–0.89) but a higher incidence of serious adverse events (3.3% vs. 1.3%, P < 0.001), including hypotension, syncope, arrhythmias, hyperkalemia, angioedema, and renal failure. The ACCORD BP findings were considered by many as conclusive evidence against the <130 mmHg …
The genomic identification of Colombian Acinetobacter baumannii clinical isolates by RFLP-PCR analysis of the 16S-23S rRNA gene spacer region

The 16S-23S rRNA gene intergenic spacer (ITS) was analysed by RFLP in this study to identify A. baumannii from 139 isolates from four hospitals (identified as A, B, C and D). One hundred and twenty of these isolates (86.3%) belonged to the A. baumannii species; those identified as being A. baumannii were found to be polyclonal (19 clone groups) when determining the genetic relationships, 16 of them being found in hospital C. Hospitals A, B and D shared two clone groups isolated during different years. This study describes a rapid and easy method for genospecies identification of Acinetobacter baumannii.
Mechanism of Hydrophobic Bile Acid-Induced Hepatocyte Injury and Drug Discovery

Cholestatic liver disease is caused by the obstruction of bile synthesis, transport, and excretion in or outside the liver by a variety of reasons. Long-term persistent cholestasis in the liver can trigger inflammation, necrosis, or apoptosis of hepatocytes. Bile acid nuclear receptors have received the most attention for the treatment of cholestasis, while the drug development for bile acid nuclear receptors has made considerable progress. However, the targets regulated by bile acid receptor drugs are limited. Thus, as anticipated, intervention in the expression of bile acid nuclear receptors alone will not yield satisfactory clinical results. Therefore, this review comprehensively summarized the literature related to cholestasis, analyzed the molecular mechanism that bile acid damages cells, and status of drug development. It is hoped that this review will provide some reference for the research and development of drugs for cholestasis treatment in the future.
The Kaiju project: enabling event-driven observability

Microservices architectures are getting momentum. Even small and medium-size companies are migrating towards cloud-based distributed solutions supported by lightweight virtualization techniques, containers, and orchestration systems. In this context, understanding the system behavior at runtime is critical to promptly react to errors. Unfortunately, traditional monitoring techniques are not adequate for such complex and dynamic environments. Therefore, a new challenge, namely observability, emerged from precise industrial needs: expose and make sense of the system behavior at runtime. In this paper, we investigate observability as a research problem. We discuss the benefits of events as a unified abstraction for metrics, logs, and trace data, and the advantages of employing event stream processing techniques and tools in this context. We show that an event-based approach enables understanding the system behavior in near real-time more effectively than state-of-the-art solutions in the field. We implement our model in the Kaiju system and we validate it against a realistic deployment supported by a software company.