Miracle-dz/UniFAR-FTUs huggingface dataset:
TITLE PAGE Title: Prognostic significance of long non-coding RNA DANCR expression in human cancers: A systematic review and meta-analysis Authors and affiliations:

35 Background: Several studies demonstrated that long non-coding RNA differentiation 36 antagonizing non-protein coding RNA (lncRNA DANCR) expression might have the 37 potential capacity to predict the cancer prognosis, however, definite conclusion has 38 not been obtained. The aim of this meta-analysis was to evaluate the prognostic value 39 of lncRNA DANCR expression in cancers. Methods: PubMed, Web of Science, 40 Scopus and Embase were comprehensively searched for relevant studies. Studies 41 meeting all inclusion standards were included into this meta-analysis. The analysis of 42 overall survival (OS), disease-free survival (DFS) or clinicopathological features was 43 conducted. Results: Eleven studies containing 1,154 cancer patients were analyzed in 44 this meta-analysis. The results showed, compared with low lncRNA DANCR 45 expression, high lncRNA DANCR expression was significantly associated with 46 shorter OS (HR=1.85, 95%CI=1.52-2.26, P<0.01) and DFS (HR=1.82, 47 95%=1.43-2.32, P<0.01) in cancers. Besides, high lncRNA DANCR expression 48 predicted deeper tumor invasion (P<0.01), earlier lymph node metastasis (P<0.01), 49 earlier distant metastasis (P<0.01) and more advanced clinical stage (P<0.01) 50 compared with low lncRNA DANCR expression in cancer populations. Conclusion: 51 High lncRNA DANCR expression was associated with worse prognosis compared 52 with low lncRNA DANCR expression in cancers. LncRNA DANCR expression could 53 serve as a prognostic factor of human cancers. 54
Reply to: “Suggestions for Vitamin D Supplementation for Urgency Urinary Incontinence Study”

dose vitamin D treatment for the prevention of functional decline: a randomized clinical trial. JAMA Intern Med. 2016;176(2):175-183. 3. Sanders KM, Stuart AL, Williamson EJ, et al. Annual high-dose oral vitamin D and falls and fractures in older women: a randomized controlled trial. JAMA. 2010;303(18):1815-1822. 4. Ross AC, Manson JE, Abrams SA, et al. The 2011 report on dietary reference intakes for calcium and vitamin D from the Institute of Medicine: what clinicians need to know. J Clin Endocrinol Metab. 2011;96:53-58. 5. Rizzoli R, Boonen S, Brandi ML, et al. Vitamin D supplementation in elderly or postmenopausal women: a 2013 update of the 2008 recommendations from the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO). Curr Med Res Opin. 2013;29(4):305-313. 6. Wacker M, Holick MF. Vitamin D effects on skeletal and extraskeletal health and the need for supplementation. Nutrients. 2013;5(1):111-148.
Network structure underpinning (dys)homeostasis in chronic fatigue syndrome; Preliminary findings

Introduction A large body of evidence has established a pattern of altered functioning in the immune system, autonomic nervous system and hypothalamic pituitary adrenal axis in chronic fatigue syndrome. However, the relationship between components within and between these systems is unclear. In this paper we investigated the underlying network structure of the autonomic system in patients and controls, and a larger network comprising all three systems in patients alone. Methods In a sample of patients and controls we took several measures of autonomic nervous system output during 10 minutes of supine rest covering tests of blood pressure variability, heart rate variability and cardiac output. Awakening salivary cortisol was measured on each of two days with participants receiving 0.5mg dexamethasone during the afternoon of the first day. Basal plasma cytokine levels and the in vitro cytokine response to dexamethasone were also measured. Symptom outcome measures used were the fatigue impact scale and cognitive failures questionnaire. Mutual information criteria were used to construct networks describing the dependency amongst variables. Data from 42 patients and 9 controls were used in constructing autonomic networks, and 15 patients in constructing the combined network. Results The autonomic network in patients showed a more uneven distribution of information, with two distinct modules emerging dominated by systolic blood pressure during active stand and end diastolic volume and stroke volume respectively. The combined network revealed strong links between elements of each of the three regulatory systems, characterised by three higher modules the centres of which were systolic blood pressure during active stand, stroke volume and ejection fraction respectively. Conclusions CFS is a complex condition affecting physiological systems. It is important that novel analytical techniques are used to understand the abnormalities that lead to CFS. The underlying network structure of the autonomic system is significantly different to that of controls, with a small number of individual nodes being highly influential. The combined network suggests links across regulatory systems which shows how alterations in single nodes might spread throughout the network to produce alterations in other, even distant, nodes. Replication in a larger cohort is warranted.
Small-Target Detection between SAR Images Based on Statistical Modeling of Log-Ratio Operator

The log-ratio (LR) operator is well suited for change detection in synthetic aperture radar (SAR) amplitude or intensity images. In applying the LR operator to change detection in multi-temporal SAR images, a crucial problem is how to develop precise models for the LR statistics. In this study, we first derive analytically the probability density function (PDF) of the LR operator. Subsequently, the PDF of the LR statistics is parameterized by three parameters, i.e., the number of looks, the coherence magnitude, and the true intensity ratio. Then, the maximum-likelihood (ML) estimates of parameters in the LR PDF are also derived. As an example, the proposed statistical model and corresponding ML estimation are used in an operational application, i.e., determining the constant false alarm rate (CFAR) detection thresholds for small target detection between SAR images. The effectiveness of the proposed model and corresponding ML estimation are verified by applying them to measured multi-temporal SAR images, and comparing the results to the well-known generalized Gaussian (GG) distribution; the usefulness of the proposed LR PDF for small target detection is also shown.
Performing group-level functional image analyses based on homologous functional regions mapped in individuals

Functional MRI (fMRI) studies have traditionally relied on intersubject normalization based on global brain morphology, which cannot establish proper functional correspondence between subjects due to substantial intersubject variability in functional organization. Here, we reliably identified a set of discrete, homologous functional regions in individuals to improve intersubject alignment of fMRI data. These functional regions demonstrated marked intersubject variability in size, position, and connectivity. We found that previously reported intersubject variability in functional connectivity maps could be partially explained by variability in size and position of the functional regions. Importantly, individual differences in network topography are associated with individual differences in task-evoked activations, suggesting that these individually specified regions may serve as the “localizer” to improve the alignment of task-fMRI data. We demonstrated that aligning task-fMRI data using the regions derived from resting state fMRI may lead to increased statistical power of task-fMRI analyses. In addition, resting state functional connectivity among these homologous regions is able to capture the idiosyncrasies of subjects and better predict fluid intelligence (gF) than connectivity measures derived from group-level brain atlases. Critically, we showed that not only the connectivity but also the size and position of functional regions are related to human behavior. Collectively, these findings suggest that identifying homologous functional regions across individuals can benefit a wide range of studies in the investigation of connectivity, task activation, and brain-behavior associations.
Physiological stress markers during breath‐hold diving and SCUBA diving

This study investigated the sources of physiological stress in diving by comparing SCUBA dives (stressors: hydrostatic pressure, cold, and hyperoxia), apneic dives (hydrostatic pressure, cold, physical activity, hypoxia), and dry static apnea (hypoxia only). We hypothesized that despite the hypoxia induces by a long static apnea, it would be less stressful than SCUBA dive or apneic dives since the latter combined high pressure, physical activity, and cold exposure. Blood samples were collected from 12SCUBA and 12 apnea divers before and after dives. On a different occasion, samples were collected from the apneic group before and after a maximal static dry apnea. We measured changes in levels of the stress hormones cortisol and copeptin in each situation. To identify localized effects of the stress, we measured levels of the cardiac injury markers troponin (cTnI) and brain natriuretic peptide (BNP), the muscular stress markers myoglobin and lactate), and the hypoxemia marker ischemia‐modified albumin (IMA). Copeptin, cortisol, and IMA levels increased for the apneic dive and the static dry apnea, whereas they decreased for the SCUBA dive. Troponin, BNP, and myoglobin levels increased for the apneic dive, but were unchanged for the SCUBA dive and the static dry apnea. We conclude that hypoxia induced by apnea is the dominant trigger for the release of stress hormones and cardiac injury markers, whereas cold or and hyperbaric exposures play a minor role. These results indicate that subjects should be screened carefully for pre‐existing cardiac diseases before undertaking significant apneic maneuvers.
Scale-dependent interactions between tree canopy cover and impervious surfaces reduce daytime urban heat during summer

Significance Cities worldwide are experiencing record-breaking summer air temperatures, with serious consequences for people. Increased tree cover is suggested as a climate adaptation strategy, but the amount of tree canopy cover needed to counteract higher temperatures associated with impervious surface cover is not known. We used a bicycle-mounted measurement system to quantify the interaction of canopy cover and impervious surface cover on urban air temperature. Daytime air temperature was substantially reduced with greater canopy cover (≥40%) at the scale of a typical city block (60–90 m), especially on the hottest days. However, reducing impervious surfaces remained important for lowering nighttime temperatures. Results can guide strategies for increasing tree cover to mitigate daytime urban heat and improve residents’ well-being. As cities warm and the need for climate adaptation strategies increases, a more detailed understanding of the cooling effects of land cover across a continuum of spatial scales will be necessary to guide management decisions. We asked how tree canopy cover and impervious surface cover interact to influence daytime and nighttime summer air temperature, and how effects vary with the spatial scale at which land-cover data are analyzed (10-, 30-, 60-, and 90-m radii). A bicycle-mounted measurement system was used to sample air temperature every 5 m along 10 transects (∼7 km length, sampled 3–12 times each) spanning a range of impervious and tree canopy cover (0–100%, each) in a midsized city in the Upper Midwest United States. Variability in daytime air temperature within the urban landscape averaged 3.5 °C (range, 1.1–5.7 °C). Temperature decreased nonlinearly with increasing canopy cover, with the greatest cooling when canopy cover exceeded 40%. The magnitude of daytime cooling also increased with spatial scale and was greatest at the size of a typical city block (60–90 m). Daytime air temperature increased linearly with increasing impervious cover, but the magnitude of warming was less than the cooling associated with increased canopy cover. Variation in nighttime air temperature averaged 2.1 °C (range, 1.2–3.0 °C), and temperature increased with impervious surface. Effects of canopy were limited at night; thus, reduction of impervious surfaces remains critical for reducing nighttime urban heat. Results suggest strategies for managing urban land-cover patterns to enhance resilience of cities to climate warming.
One of the Boys? Gender Similarities and Differences in Human Capital among Senior Civil Servants in Denmark

Studies have shown how women are underrepresented in senior executive positions in public and private organizations. Equal representation matters both for reasons of performance and legitimacy, and to understand the mechanisms behind the glass ceiling, we explore if the women making it to the top of the Danish civil service differ from the men who do so. We want to understand if senior executive positions require something different of women than men. Using a dataset consisting of the entire career trajectory of all senior civil servants in Denmark, we find that, on numerous human capital dimensions, the women and men making it to the top are quite similar, e.g. in terms of tenure and educational level However, we find on the one hand that men are more often employed in the most prestigious departments and at the same time, it seems that men with a profile deviating from the norm are more likely to make it to the top than women. This may indicate that the most prestigious positions – also in terms of early-career positions – are less accessible to women, and that women are less willing to apply for jobs outside their usual domain, or that those responsible for recruitment are less willing to take a chance on a woman with a slightly unorthodox profile. Hence, our study indicates that greater interest should be paid to the dynamics keeping women at lower levels of the hierarchy and possibly to encourage them to apply for top positions.
Rural-urban differences in college students’ cardiovascular risk perceptions

Abstract Objective: To examine rural-urban differences in college students’ cardiovascular risk perceptions. Participants: College students in rural (n = 61) and urban (n = 57) Kentucky counties were recruited from November 2012 to May 2014. Methods: This was a secondary data analysis of a cross-sectional study examining rural-urban differences in cardiovascular risk factors. Students rated their risk for developing high blood pressure, diabetes, high cholesterol, heart disease, having a stroke, and gaining excess weight. Chi-square and logistic regression were used for data analysis. Results: Rural students had lower odds of perceived high risk for developing high blood pressure compared to urban students (odds ratio (OR): 0.32, 95% CI: 0.11–0.96) after adjusting for race, sex, and body mass index. This association was not observed after adjusting for healthcare access variables. No other significant differences were observed. Conclusions: Efforts to raise perceived risk for developing hypertension among rural college students may be warranted.
Metabolism of Stilbenoids by Human Faecal Microbiota

Stilbenoids are dietary phenolics with notable biological effects on humans. Epidemiological, clinical, and nutritional studies from recent years have confirmed the significant biological effects of stilbenoids, such as oxidative stress protection and the prevention of degenerative diseases, including cancer, cardiovascular diseases, and neurodegenerative diseases. Stilbenoids are intensively metabolically transformed by colon microbiota, and their corresponding metabolites might show different or stronger biological activity than their parent molecules. The aim of the present study was to determine the metabolism of six stilbenoids (resveratrol, oxyresveratrol, piceatannol, thunalbene, batatasin III, and pinostilbene), mediated by colon microbiota. Stilbenoids were fermented in an in vitro faecal fermentation system using fresh faeces from five different donors as an inoculum. The samples of metabolized stilbenoids were collected at 0, 2, 4, 8, 24, and 48 h. Significant differences in the microbial transformation among stilbene derivatives were observed by liquid chromatography mass spectrometry (LC/MS). Four stilbenoids (resveratrol, oxyresveratrol, piceatannol and thunalbene) were metabolically transformed by double bond reduction, dihydroxylation, and demethylation, while batatasin III and pinostilbene were stable under conditions simulating the colon environment. Strong inter-individual differences in speed, intensity, and pathways of metabolism were observed among the faecal samples obtained from the donors.
Transfer Spectral Entropy and Application to Functional Corticomuscular Coupling

Functional corticomuscular coupling (FCMC) with different rhythmic oscillations plays different roles in neural communication and interaction between the central nervous system and the peripheral system. Larger methods, such as coherence and Granger causality (GC), have been used to describe the frequency band characteristics in the frequency domain, but they fail to account for the inherent complexity. Considering that the transfer entropy (TE) method as an information theory has advantages in complexity and direction, we extended it and proposed a novel method named transfer spectral entropy (TSE) to explore the local frequency band characteristics between two coupling signals. To verify this, we introduced a Henon model and a neural mass model to generate the simulation signals. We then applied the proposed method to explore the FCMC by analyzing the correlation between the EEG and EMG signals during steady-state force output. Simulation results showed that the TSE method, compared with the GC method, not only described the information interaction in the local frequency band but also restrained the “false coupling. ” In addition, the results also revealed that the TSE method was sensitive to coupling strength but not to the data length. Further analysis of the experimental data showed that beta1 (15–25 Hz) and beta2 (25–35 Hz) bands were prominent in the FCMC for both EEG-to-EMG and EMG-to-EEG directions. In addition, the statistical analysis of the significant area indicated that the coupling in the EEG-to-EMG direction was higher at the beta1 and beta2 bands than that in the EMG-to-EEG direction, and the coupling in the EMG-to-EEG direction was higher at the gamma1 band (35–45 Hz) than that in the opposition. The FCMC results complementarily refined the previous studies that mainly focused on the beta band (15–35 Hz). The simulation and experimental data expound the effectiveness of the TSE model to describe the information interaction in the local frequency band between two time series, and this study extends the relative studies on FCMC.
Growth and Proliferation of Renal Cell Carcinoma Cells Is Blocked by Low Curcumin Concentrations Combined with Visible Light Irradiation

The anti-cancer properties of curcumin in vitro have been documented. However, its clinical use is limited due to rapid metabolization. Since irradiation of curcumin has been found to increase its anti-cancer effect on several tumor types, this investigation was designed to determine whether irradiation with visible light may enhance the anti-tumor effects of low-dosed curcumin on renal cell carcinoma (RCC) cell growth and proliferation. A498, Caki1, and KTCTL-26 cells were incubated with curcumin (0.1–0.4 µg/mL) and irradiated with 1.65 J/cm2 visible light for 5 min. Controls were exposed to curcumin or light alone or remained untreated. Curcumin plus light, but not curcumin or light exposure alone altered growth, proliferation, and apoptosis of all three RCC tumor cell lines. Cells were arrested in the G0/G1 phase of the cell cycle. Phosphorylated (p) CDK1 and pCDK2, along with their counter-receptors Cyclin B and A decreased, whereas p27 increased. Akt-mTOR-signaling was suppressed, the pro-apoptotic protein Bcl-2 became elevated, and the anti-apoptotic protein Bax diminished. H3 acetylation was elevated when cells were treated with curcumin plus light, pointing to an epigenetic mechanism. The present findings substantiate the potential of combining low curcumin concentrations and light as a new therapeutic concept to increase the efficacy of curcumin in RCC.
In vitro relationship between serum protein binding to beta-carboline alkaloids: a comparative cytotoxic, spectroscopic and calorimetric assays

Abstract The work highlighted interaction of harmalol, harmaline and harmine with human serum albumin by biophysical and biochemical assays. Presence of serum protein in the media negatively affects the cytotoxicity of the alkaloids. MTT assay indicates concentration-dependent growth inhibitory effect of the alkaloids on A375, MDA-MB-231, HeLa, A549, ACHN and HepG2 cell, having maximum cytotoxicity with GI50 value of 6.5 μM on ACHN by harmine in 1% of fetal bovine serum. Detail cytotoxic studies on ACHN cell by harmine, the most cytotoxic among the three, reveal nucleosomal fragmentation, formation of comet tail, generation of reactive oxygen species, decreased mitochondrial membrane potential, up regulation of p53, caspase 3 and significant increase in G2/M population that made the cancer cells prone to apoptosis. Furthermore, the findings unequivocally pointed out that harmine binds strongly to the protein with a binding constant of 5.53 × 104 M−1 followed by harmaline and least with harmalol. Thermodynamic results revealed enthalpy dominated, entropy favored, 1:1 binding. Molecular docking and circular dichroism suggested changed conformation of protein by partial unfolding on complexation. Further supported by infrared analysis where protein secondary structure was altered with a major decrease of α-helix from 53.68% (free protein) to 8–11% and change in β-sheet from 25.31% (free protein) to 1–6% upon binding, inducing partial protein destabilization. Site markers demonstrated site I (subdomain IIA) binding of the alkaloids to the protein. The results serve as data for the future development of serum protein-based targeted drugs. Abbreviations CD: circular dichroism; FBS: fetal bovine serumFRET Forster resonance energy transferFTIR Fourier transform infraredHSA human serum albumin; ROS: reactive oxygen species Communicated by Ramaswamy H. Sarma
Regulation of p38MAPK‐mediated dendritic cell functions by the deubiquitylase otubain 1

Dendritic cells (DCs) are professional antigen presenting cells (APCs) that represent the essential link between innate and acquired immunity. Otubain (OTUB) 1 is shown to deubiquitinate TRAFs to suppress virus‐induced inflammatory response. MAPK, a downstream molecule of TRAFs, is involved in regulating LPS‐induced immune reactions and its activation is sensitive to the presence of OTUB1. Little is known about contributions of OTUB1 to changes in biological properties of DCs. The present study, therefore, explored whether DC functions are influenced by OTUB1. To this end, DCs were isolated and cultured with GM‐CSF to attain bone marrow‐derived DCs (BMDCs) and followed by treatment with lipopolysaccharide (LPS) in the presence or absence of OTUB1 siRNA. Expression of markers of cellular maturation and proliferation were analyzed by flow cytometry, and secretion of inflammatory cytokines and ability to stimulate CD4+ T‐cells in allogenic mixed leukocyte reaction (allo‐MLR) by ELISA, cell migration by a transwell migration assay and phagocytic capacity by FITC‐dextran uptake measurement. As a result, treatment of the cells with OTUB1 siRNA prolonged activation of p38MAPK, increased CD54 expression and IL‐6 release and reduced FITC‐dextran uptake. Moreover, cytokine release produced from CD4+ T‐cells in allo‐MLR was different. The enhanced level of IFN‐γ, but not other cytokine production was observed in the presence of siRNA OTUB1. All the effects were completely abolished when the cells were exposed with p38MAPK inhibitor SB203580. In conclusion, OTUB1 prevents the prolonged activation of p38MAPK, which in turn compromises DC functions.