Miracle-dz/UniFAR-FTUs huggingface dataset:
Optimization of Fermentation Medium for the Production of Recombinant Human Antithrombin III from Saccharomyces cerevisiae through Statistical Experimental Designs.

This work, for the first time reports the optimization of recombinant human antithrombin (rhAT) production in Saccharomyces cerevisiae BY4741 using statistical experimental designs. Most applications of design of experiments (DoE) have concerned optimization of the composition of growth and production culture media. The typical objective is to identify a best selection and quantitative composition of significant medium supplements. In the present study, Plackett- Burman (PB) design followed by central composite design has been employed to evaluate and optimize the suitable culture medium for rhAT production. Influence of raffinose, glutamic acid and vitamin mixture were screened to be significant variables by PB design. The significant nutritional variables were further optimized using central composite design (CCD) for maximum production of rhAT. Central composite design (CCD) has been selected to explain the interaction effect of the three significant variables such as raffinose, glutamic acid, and vitamin mixture. The multiple regression equation (R2=0.9967) was used to optimize significant impact of medium component values to maximize rhAT formation. The optimized values were found to be 23.09765 g/L, 8.01816 g/L and 77.2056 mg/L for raffinose, glutamic acid and vitamin mixture, respectively. The maximum yield of rhAT of 38.97 µg/mL was obtained experimentally using CCD and was very close to the predicted response of 38.93 µg/mL.
Valor nutritivo da leguminosa Flemingia macrophylla (Willd.) Merrill para suplementação alimentar de ruminantes na Amazônia Oriental

The work was carried out at the Embrapa Eastern Amazon, in Belem, Para State, Brazil (1o28’S 48o27’W). The nutritional characteristics of the legume Flemingia macrophylla were determined, during 21 days, with 16 sheeps, in individual metabolic cages, distributed in a completely randomized design, with four treatments and four repetitions. The experimental treatments (T1, T2, T3 and T4) contained Brachiaria humidicola and decreasing levels (100%, 75%, 50% and 25%) of inclusion of F. macrophylla. The consumption of dry matter (g/day) and % of live weight were 901.8 and 2.4; 947.9 and 2.5; 859.5 and 2.2; e 930.2 and 2.5 and of crude protein 232.4; 188.7; 132.1 and 107.6 g/day. The coefficients of digestibility were 54.1; 59.2; 55.1; e 62.0%, in dry matter, and 57.3; 61.2; 57.6; e 64.0% in organic matter and the crude protein were 63.2; 60.5; 51.4; 52.0%, respectively. The tannin level in the diet was 1.37; 0.62; 0.31 and 0.17%, respectively. The legume has high feeding potential, with high availability of dry matter, to be used as an alternative for supplementary feeding of ruminants, mainly in critical periods. The level of inclusion of F. macrophylla around 75%, make possible higher consumption of dry matter, organic matter and of fiber fractions and 100% allow better consumption of crude protein and gross energy.
Software development project states and project behavior

The behavior of software development projects is a characteristic phenomenon that is difficult to study and analyze. The behavior of a software project is a true indicator of its progress and condition. Unfortunately, very less attention has been given to this important aspect of the software projects and rarely any research study has been presented in this regard. The present study addresses this limitation of existing research work and analyzes the behavior of software development projects based on the project states. Project states and their sub-states for the first time in the present study have been derived from the project lifecycle phases. The behavior of the project states have been analyzed based on the risks faced by the projects and project client factors. A case study of real projects has been conducted to validate the project states and their behavior. The project states reflect the true behavior of the software development projects and determine the status and progress of the project. Initial findings of the study have been presented in this paper which will be followed by publishing more detailed findings on the project states in upcoming paper.
Comparison of the CardioShield filter with the guardwire balloon in the prevention of embolisation during vein graft intervention: results from the CAPTIVE randomised trial.

AIMS This study assessed safety and efficacy of a third-generation distal protection device, MedNova CardioShield Bare Wire Myocardial Protection System, for treating Saphenous Vein Graft (SVG) disease.Treatment of SVG disease remains difficult, with increased adverse cardiac events (MACE) primarily manifested as no reflow and periprocedural infarction. Even with approved embolic protection devices, 30-day MACE rates are approximately 10%. METHODS AND RESULTS A multicentre randomized clinical trial evaluated a third-generation distal protection device MedNova CardioShield vs Percusurge GuardWire in 652 patients undergoing treatment of SVG disease, using a primary endpoint of 30-day death, Q-wave, non-Q-wave infarction, or target vessel revascularisation (MACE). The primary endpoint occurred in 11.4% with CardioShield vs 9.1% with GuardWire (P=.37). Intention-to-treat analysis showed a strong trend for noninferiority (P=.057). Secondary modified intention-to-treat analysis including only patients receiving treatment device and no protocol deviation (defined as treatment of another lesion not using embolic protection) supported noninferiority of CardioShield (P=.022). CONCLUSION Analysis of outcomes of treatment strategies for SVG disease is difficult. In this trial, final results depended on whether a patient actually received the device according to protocol. With 30-day MACE as primary endpoint, CardioShield was not demonstrated to be noninferior to GuardWire.
Combination Therapy for the Treatment and Prevention of Hepatic Encephalopathy

OBJECTIVE: To evaluate the efficacy and safety of combination therapy for the treatment and prevention of hepatic encephalopathy (HE). DATA SOURCES: A PubMed MEDLINE search was conducted (1947-June 2012) using the key terms lactulose, lactitol, nonabsorbable disaccharide, metronidazole, rifaximin, neomycin, probiotics, and hepatic encephalopathy. Searches were limited to include articles published in English. STUDY SELECTION AND DATA EXTRACTION: Study selection included published trials, case reports, and case series of humans with HE who were treated with combination therapy of rifaximin, lactulose, lactitol, metronidazole, neomycin, and/or probiotics. DATA SYNTHESIS: Only 6 studies that evaluated the benefits of combination drug therapy in the treatment or prevention of HE were available for review. Four studies addressed the treatment of HE, 2 found no significant difference between lactulose/neomycin versus placebo or rifaximin/lactulose, 1 assessed the use of rifaximin/lactulose without a control group, and the fourth found no significant difference between lactulose/probiotics versus either drug alone, although each group showed improvement from baseline. In the 2 prevention trials, both of which stemmed from the same data, the combination of rifaximin/lactulose was superior to lactulose alone, showing significant improvement in mental status, blood ammonia levels, and health-related quality of life and reductions in HE recurrence and hospitalization. Currently, there are no available clinical studies evaluating dual antibiotic therapy, metronidazole with nonabsorbable disaccharides, or antibiotics with probiotics. CONCLUSIONS: The evidence evaluating the use of combination therapy for the treatment of HE does not support its widespread use. The combination of rifaximin and lactulose may be considered in the treatment of HE and in patients refractory to monotherapy. The combination of rifaximin and lactulose should be considered for the prevention of HE, especially after the second episode of HE recurrence.
A method of optimal control for switched-mode power converters

An optimal control method allowing to achieve the high dynamic performances of switched-mode power converters described by the second-order difference equation is offered. To this purpose, control vector is formed as a linear combination of differences of system variables, and matrix transformation of the linear equation to the nilpotent form is implemented. This control approach gives an ability to achieve a convergence of transient in a fixed point within two steps and to remain this property under regulation and perturbation of system parameters. Matrix transformation of the equation to the nilpotent form is realized by the system solution of the linear algebraic equations regarding the multipliers at the differences of system state variables. A proposed control method can be used as a basis for development of systems with as much as possible high dynamic behavior. To confirm the efficiency of this method, the system for load current control of a PWM buck converter is resulted as an example of the switched-mode power supply.
Assessment of cytoskeleton and endoplasmic reticulum of fibroblast cells subjected to low-level laser therapy and low-intensity pulsed ultrasound.

OBJECTIVE The aim of the present study was to compare the effect of low-level laser therapy (LLLT) and low-intensity pulsed ultrasound (LIPUS) on the cytoskeleton and endoplasmic reticulum of L929 cells. Thermal and non-thermal physical mechanisms such as LLLT and LIPUS induce clinically significant responses in cells, tissues, and organs. MATERIALS AND METHODS L929 fibroblast cell cultures were irradiated with LLLT and subjected to LIPUS. Cultures irradiated with the laser (904 nm) were divided into three groups: group I, control (no irradiation); group II, irradiated at 6 J/cm(2); and group III, irradiated at 50 mJ/cm(2). Cultures subjected to ultrasound were divided into five groups: group I, control (no LIPUS); group II, LIPUS at 0.2 W/cm(2) in pulsed mode at 10% (1:9 duty cycle); group III, LIPUS at 0.6 W/cm(2) in pulsed mode at 10% (1:9 duty cycle); group IV, LIPUS at 0.2 W/cm(2) in pulsed mode at 20% (2:8 duty cycle); and group V, LIPUS at 0.6 W/cm(2) in pulsed mode at 20% (2:8 duty cycle). Each group was irradiated at 24-h intervals, with the following post-treatment incubation times: 24, 48, and 72 h. The effects of LLLT and LIPUS on the cytoskeleton and endoplasmic reticulum was evaluated by the use of fluorescent probes and with fluorescence microscopy analysis. RESULTS The results following LLLT and LIPUS demonstrate that ultrasound was more effective than laser on fibroblast cell cultures when the endoplasmic reticulum was assessed, whereas there was a better distribution of the filaments of the cytoskeleton in the cells subjected to laser irradiation. CONCLUSION The study demonstrated that both LLLT and LIPUS promote changes on the cellular level. However, LIPUS was more effective than LLLT at the doses used here, as assessed by fluorescence microscopy, which revealed increased reticulum activity and increased protein synthesis. However, when the organization of actin filaments was assessed, LLLT achieved a better result.
Monitoring the Indian Summer Monsoon Evolution at the Granularity of the Indian Meteorological Sub-divisions using Remotely Sensed Rainfall Products

We make use of satellite-based rainfall products from the Tropical Rainfall Measuring Mission (TRMM) Multi-satellite Precipitation Analysis (TMPA) to objectively define local onset and demise of the Indian Summer Monsoon (ISM) at the spatial resolution of the meteorological subdivisions defined by the Indian Meteorological Department (IMD). These meteorological sub-divisions are the operational spatial scales for official forecasts issued by the IMD. Therefore, there is a direct practical utility to target these spatial scales for monitoring the evolution of the ISM. We find that the diagnosis of the climatological onset and demise dates and its variations from the TMPA product is quite similar to the rain gauge based analysis of the IMD, despite the differences in the duration of the two datasets. This study shows that the onset date variations of the ISM have a significant impact on the variations of the seasonal length and seasonal rainfall anomalies in many of the meteorological sub-divisions: for example, the early or later onset of the ISM is associated with longer and wetter or shorter and drier ISM seasons, respectively. It is shown that TMPA dataset (and therefore its follow up Global Precipitation Measurement (GPM) Integrated Multi-satellite Retrievals for GPM (IMERG)) could be usefully adopted for monitoring the onset of the ISM and therefore extend its use to anticipate the potential anomalies of the seasonal length and seasonal rainfall anomalies of the ISM in many of the Indian meteorological sub-divisions.
Nuestro cambiante mundo y la pérdida de diversidad biológica

En este articulo se pretende abordar algunos temas utiles para entender los problemas de extincion a los que se enfrentan los diversos grupos biologicos. En primer lugar se presenta un panorama de la gran diversidad biologica de nuestro planeta, asi como del escaso conocimiento que tenemos de la misma. El mundo es dinamico, por lo que el numero de especies presentes en un area determinada es resultado tanto de los procesos de formacion de nuevas especies (especiacion) como de las extinciones de las especies que fueron incapaces de adaptarse a cambios ambientales. Los cambios constantes de la superficie terrestre y la presencia de eventos catastroficos, son causas naturales de las extinciones. Las especies especializadas o especialistas, asi como las especies endemicas (con un restringido rango de distribucion), tienen mayores probabilidades de extinguirse, debido a que tienen menores probabilidades de adecuacion a los cambios ambientales. La extincion de las especies por causas antropogenicas, esta intimamente ligada a la evolucion misma del hombre y su civilizacion. Las principales causas antropogenicas de la extincion de las especies, son: a) Persecucion directa de organismos; b) Introduccion de especies exoticas, y c) Alteracion de los habitats. La perdida masiva de diversidad biologica es cada dia mas probable. Con ella se presentara la reduccion de muchos servicios esenciales para las sociedades humanas. Es una ilusion pensar que podemos sobrevivir sin la diversidad biologica o que de alguna manera es secundaria en nuestro mundo contemporaneo. Entre las razones para conservar la biodiversidad, existen argumentos de tipo economico y de mantenimiento de la calidad ambiental (servicios de los ecosistemas), asi como razones eticas y esteticas. La calidad de vida de las generaciones futuras dependera, en gran medida, de las decisiones y las acciones que todos y cada uno de no
A Review on Road Accident in Traffic System using Data Mining Techniques

: Road traffic accidents are a major public health concern, resulting in an estimated 1.2 million deaths and 50 million injuries worldwide each year. In the developing world, road traffic accidents are among the leading cause of death and injury. The objective of this study is to evaluate a set of variable that contribute to the degree of accident severity in traffic crashes. The issue of traffic safety has raised great concerns across the sustainable development of modern traffic and transportation. The study on road traffic accident cause can identify the key factors rapidly, efficiently and provide instructional methods to the traffic accident prevention and road traffic accident reduction, which could greatly reduce personal casualty by road traffic accidents. Using the methods of traffic data analysis, can improve the road traffic safety management level effectively.
Analysis of Immunological Nonresponsiveness to the 19-Kilodalton Fragment of Merozoite Surface Protein 1 of Plasmodium yoelii: Rescue by Chemical Conjugation to Diphtheria Toxoid (DT) and Enhancement of Immunogenicity by Prior DT Vaccination

ABSTRACT The Plasmodium merozoite surface protein 1 (MSP1) is a leading vaccine candidate for protecting against the blood stage of malaria. Previous studies have shown that the 19-kDa carboxyl terminus of this protein is able to induce protective immunity in some monkey and mouse strains. We show that immunization with the recombinant Plasmodium yoelii 19-kDa fragment of MSP1 (MSP119) expressed in Saccharomyces cerevisiae (yMSP119) can induce protective antibodies in several inbred mouse strains and one outbred mouse strain. However, mice expressing the H-2s major histocompatibility complex haplotype are unable to generate yMSP119-specific antibodies. While synthetic peptides derived from MSP119 are immunogenic in B10.S mice, they cannot function as helper epitopes, and immunization with yMSP119 does not induce T cells that recognize the recombinant protein or synthetic peptides corresponding to its sequence. Nonresponsiveness could be overcome by using chemical linkers to conjugate yMSP119 to diphtheria toxoid (DT), resulting in immunogens capable of inducing protective yMSP119-specific antibodies in both MSP119-responsive and otherwise nonresponsive mouse strains. The ability of sera from mice immunized with the conjugate to inhibit binding of a protective monoclonal antibody (MAb 302) to yMSP119 correlated strongly with a delay in the prepatent period. Chemical conjugation of yMSP119 to DT may be a preferred method to enhance immunogenicity, as carrier priming experiments demonstrated that an existing immune response to DT enhanced a subsequent antibody response to yMSP119 after vaccination with yMSP119-DT. These results have important implications for the development of a malaria vaccine to protect a population with diverse HLAs.
Serving Ireland's Geospatial Information as Linked Data

We present data.geohive. ie, which aims to provide an authoritative platform for serving Ireland’s national geospatial data, including Linked Data. Currently, the platform provides information on Irish administrative boundaries and was designed to support two use cases: serving boundary data of geographic features at various level of detail and capturing the evolution of administrative boundaries. We report on the decisions taken for modeling and serving the data such as the adoption of an appropriate URI strategy, the development of necessary ontologies, and the use of (named) graphs to support aforementioned use cases.
Graphitic Petal Electrodes for All‐Solid‐State Flexible Supercapacitors

The charge storage characteristics of a composite nanoarchitecture with a highly functional 3D morphology are reported. The electrodes are formed by the electropolymerization of aniline monomers into a nanometer‐thick polyaniline (PANI) film that conformally coats graphitic petals (GPs) grown by microwave plasma chemical vapor deposition (MPCVD) on conductive carbon cloth (CC). The hybrid CC/GPs/PANI electrodes yield results near the theoretical maximum capacitance for PANI of 2000 F g−1 (based on PANI mass) and a large area‐normalized specific capacitance of ≈2.6 F cm−2 (equivalent to a volumetric capacitance of ≈230 F cm−3) at a low current density of 1 A g−1 (based on PANI mass). The specific capacitances remain above 1200 F g−1 (based on PANI mass) for currents up to 100 A g−1 with correspondingly high area‐normalized values. The hybrid electrodes also exhibit a high rate capability with an energy density of 110 Wh kg−1 and a maximum power density of 265 kW kg−1 at a current density of 100 A g−1. Long‐term cyclic stability is good (≈7% loss of initial capacitance after 2000 cycles), with coulombic efficiencies >99%. Moreover, prototype all‐solid‐state flexible supercapacitors fabricated from these hybrid electrodes exhibit excellent energy storage performance.
Role of the Differentially Spliced Carboxyl Terminus in Thromboxane A2 Receptor Trafficking

The thromboxane A2 receptor (TP) is a G protein-coupled receptor that is expressed as two alternatively spliced isoforms, α (343 residues) and β (407 residues) that share the first 328 residues. We have previously shown that TPβ, but not TPα, undergoes agonist-induced internalization in a dynamin-, GRK-, and arrestin-dependent manner. In the present report, we demonstrate that TPβ, but not TPα, also undergoes tonic internalization. Tonic internalization of TPβ was temperature- and dynamin-dependent and was inhibited by sucrose and NH4Cl treatment but unaffected by wild-type or dominant-negative GRKs or arrestins. Truncation and site-directed mutagenesis revealed that a YX 3φ motif (whereX is any residue and φ is a bulky hydrophobic residue) found in the proximal portion of the carboxyl-terminal tail of TPβ was critical for tonic internalization but had no role in agonist-induced internalization. Interestingly, introduction of either a YX 2φ or YX 3φ motif in the carboxyl-terminal tail of TPα induced tonic internalization of this receptor. Additional analysis revealed that tonically internalized TPβ undergoes recycling back to the cell surface suggesting that tonic internalization may play a role in maintaining an intracellular pool of TPβ. Our data demonstrate the presence of distinct signals for tonic and agonist-induced internalization of TPβ and represent the first report of a YX 3φ motif involved in tonic internalization of a cell surface receptor.
Mobile Data Offloading in Heterogeneous Networks for Passengers on a Subway Train

Mobile data offloading benefits both end users and content providers for enhancing user experiences and more data cost effectiveness, thus attracted lots of researchers' efforts on studying new offloading opportunities and optimized solutions. However, it is still under-explored in subway environment and this comes more valuable as more users are taking subway as daily means of transport. Indeed, motivated by special data offloading opportunities found in a subway train environment for users, we designed a local data distribution model and a super node selection algorithm based on context information and node resources, by combining the characteristics of users' interests on various contents, users' behavior and resources availability. Simulation results clearly show the high efficiency of our data distribution model and super node selection algorithm for offloading cellular data by as high as 90%.
'Laba' garlic processed by dense phase carbon dioxide: the relation between green colour generation and cellular structure, alliin consumption and alliinase activity.

BACKGROUND 'Laba' garlic is usually processed by soaking garlic in vinegar for more than 1 week during winter. It is popular for its unique green colour and tasty flavour. Greening is desirable and required for this product as its characteristic. Dense phase carbon dioxide (DPCD) had a significant effect on the greening of intact garlic (Allium sativum L.) cloves. The relation between green colour generation and alliin consumption, alliinase activity and the cellular structure of garlic, respectively, were investigated in this work. The effects of treatment time, pressure and temperature of DPCD were also analysed and discussed. RESULTS DPCD had a significant effect on the cellular structure of garlic cells. Garlic protoplast underwent greater morphological change after DPCD treatments at higher temperatures while the amount of precipitate increased with greater treatment time and temperature. Common trends on garlic greening and alliin consumption were observed except for DPCD treatment at 10 MPa and 65 °C. The alliinase activity decreased with increasing treatment time, pressure and temperature. It reached the lowest level at 13 MPa and 55 °C. CONCLUSION The formation of the green colour was a comprehensive result of DPCD on changing cellular structure, alliin consumption and alliinase activity. DPCD treatment at 10 MPa and 55 °C was the optimum condition for the greening of 'Laba' garlic. This work further facilitated the application of DPCD in the industrial production of 'Laba' garlic. © 2015 Society of Chemical Industry.