Miracle-dz/UniFAR-FTUs huggingface dataset:
An anticancer composition.

The present invention relates to an anticancer composition for treating cancer. The anticancer composition comprises of an active ingredient having an effective amount of Vigna radiata L. extract. The Vigna radiata L. extract having anticancer activity against cervical cancer cell and hepatocarcinoma cell. The Vigna radiata sprout.
Lateral knee pain: A comprehensive sonographic spectrum of pathologies and guided interventions

Poster: "ECR 2019 / C-2920 / Lateral knee pain: A comprehensive sonographic spectrum of pathologies and guided interventions" by: "P. jain1, D. K. Singh2, N. KUMAR1, B. K. Nayak1, A. Katyan1, S. Suman3, S. Tomar3, S. B. Grover3; 1New Delhi , Delhi/IN, 2delhi/IN, 3New Delhi/IN"
Nanostructure‐Initiator Mass Spectrometry (NIMS) for the Analysis of Enzyme Activities

Enzymes are essential to innumerable vital processes in all fields of life. Additionally, enzymatic activities are utilized in a multitude of biological, medical, and technical applications. Using cellulases and other glycoside hydrolases for the conversion of lignocellulosic biomass into biofuel has become an active area of research for applied enzymology. Development of high‐throughput, high‐specificity enzyme assays for glycoside hydrolases is critical to identify, characterize, and optimize such activities for efficient biomass degradation. Here, we present a detailed protocol describing our recently reported nanostructure‐initiator mass spectrometry (NIMS)‐based technique for the rapid analysis of glycoside hydrolase activities. NIMS enzyme activity (Nimzyme) assays can be used to characterize already known, or identify novel glycoside hydrolases. Importantly, several enzymatic activities can be tested in parallel in a multiplexed arrangement and under a broad range of assay conditions. Curr. Protoc. Chem. Biol. 4:123‐142 © 2012 by John Wiley & Sons, Inc.
Chronic amiodarone remodels expression of ion channel transcripts in the mouse heart

AIM: The basis for the unique effectiveness of chronic amiodarone on cardiac arrhythmias is incompletely understood. The present study investigated the pharmacogenomics profile of amiodarone on genes encoding ion channel subunits. METHODS AND RESULTS: Adult male mice were treated for 6 weeks with vehicle or oral amiodarone at 30,90,or 180 mg·kg^-1·d^-1, Plasma and myocardial levels of amiodarone and n-desethyl-amiodarone in
Environmental factors determine numbers of over-wintering European Stonechats Saxicola rubicola - A long term study

In this study the relationship between the number of European Stonechats Saxicola rubicola at a winter site in Israel and the local rainfall, air temperature, and arthropod abundance was examined. In some years population size declined to about one third of the initial size, while in others it remained almost constant. The decline of bird numbers was mainly due to the disappearance of young, unpaired individuals. The magnitude of the decline in numbers was correlated with timing and amount of winter rainfall, which is crucial for the growth of the vegetation after a long dry summer period. Early winter rainfalls had a much stronger impact than total winter rain. The decline in numbers was also correlated with arthropod density in pitfall traps; however, it was not correlated with air temperature. The relative extend to which density-dependent and density-independent factors determine the size of the wintering population of European Stonechats is evaluated.
Viabilidade Econômica de Sistemas Silvipastoris com Schizolobium parahyba var. amazonicum e Tectona grandis no Pará

Os sistemas silvipastoris (SSPs) consistem de um conjunto de metodologias que podem ser consideradas promissoras para recuperar areas de pastagens degradadas da Amazonia, por integrar o cultivo arboreo ao pecuaria tradicional. Embora ja utilizados em pequena escala por produtores inovadores, os SSPs carecem de uma base cientifica que forneca subsidios tecnicos ao seu uso mais amplo, sobretudo quando se trata de justificar a viabilidade economica desses sistemas. O objetivo desse estudo foi analisar a viabilidade economica de SSPs utilizados por produtores no nordeste do Estado do Para, comparando-os ao monocultivo tradicional. Os sistemas sao compostos basicamente pelo parica (Schizolobium parahyba var. amazonicum (Huber ex Ducke) Barneby) ou teca (Tectona grandis L.F.), em pastagens de quicuio-da-amazonia (Brachiaria humidicola (Rendle) Schweick) pastejados por bovinos. A analise de viabilidade economica foi realizada por meio dos metodos: valor presente liquido (VPL), taxa interna de retorno (TIR) e relacao beneficio/custo (Rb/c), considerando quatro SSPs e o monocultivo de parica e teca. Os sistemas compostos por teca apresentam maior atratividade economica do que os com parica, e dentre eles, os arranjos silvipastoris, em que o gado era proprio, foram considerados de melhor viabilidade que os sistemas em monocultivo ou em que a incorporacao animal se dava atraves do aluguel da pastagem. doi: 10.4336/2009.pfb. 60.49
Impact of mature cow weight on farm profitability and economic weights of beef cattle traits.

Impact of mature cow weight on farm profitability and economic weights of beef cattle traits The impact of mature cow weight on the profitability of beef cattle farming and on the economic importance of 10 performance and functional traits was analyzed. The examined traits were: calving performance, stillbirth and calf losses till weaning, weight of calves at birth, at 120 and at 205 days of age, mature weight of cows, conception rates of heifers and cows and productive lifetime of cows. The cow weight was varied from 500 to 700 kg in 50 kg intervals. The economic efficiency of all farming systems was expressed as profit per cow and year. The economic importance of a trait (marginal economic value) KELLER et al.: Einfluss des Kuhgewichts auf die Betriebsrentabilität und auf die ökonomischen Gewichte 256 was defined as partial derivative of the profit function with respect to trait mean. The program package ECOWEIGHT was used for all calculations. The results showed that beef cattle farming with all cow weight classes could be profitable when including subsidies in the incomes of a farm. Without subsidies, a positive profitability can be reached only when keeping small-framed cows (500 to 550 kg). In all modelled production systems, the most important trait was conception rate of cows followed by weaning weight of calves (at 205 days of age) for light cows or productive lifetime of cows in systems with heavy cows.
Impact of motivation on workplace performance: a work model for millennials

Motivation is and has always been the cardinal talk of discussion for management of any organisation. With Millennials joining the organisations, it’s becoming difficult for employers to retain them. An organisation invests its resources in molding and shaping its employees and so is always interested in retaining its employees. Millennials can be retained by motivating them to the core and bringing out best in them. There are different needs of an employee, which if fulfilled keeps their working morale high. This paper proposes a motivation model which is based on the factors affecting the performance of an employee at his workplace. The building blocks of this model are the ERG theory of needs. The new formed model defines factors which are a combination of financial and non financial incentives, responsible for boosting motivation of an employee. The study also tries to identify the prominent underlying factors responsible for increasing the level of motivation. Following a descriptive methodology, the study uses a structured questionnaire in which the data was recorded from a sample of 100 Millennials belonging to different age group of employees. The study attempts to find the preference of type of incentives preferred by employees to keep them motivated.
Relation between Stress and Coping Strategies as Perceived by Nursing Students during Clinical Practice

Background: Nursing training is a stressful process. The effects of stress on nursing students have been well documented. High perceived stress levels and lack of effective coping processes could be a barrier to achieve the challenges of profession. Aim of the study: Assess relation between stress and coping strategies as perceived by nursing students during clinical practice. Design: a descriptive design was utilized to conduct this study. Setting: the current study was conducted at Technical Institute of Nursing, Zagazig University, Sharkia Governorate. Sample: a cluster random sample of 221 nurse students were included in this study. Tools: Three tools were used to collect data: demographic data questionnaire & Student Nurses Stress Index, Brief Cope Inventory and Emotional Approach Coping Scale. Results: More than three quarters of the studied sample had low total stress, all of them used low emotional-oriented coping and more than two thirds of studied nurse students used low problem-oriented coping. A highly positive significant correlation was detected between nurse students’ stress and emotion-oriented coping and emotion processing. Also, a highly positive significant correlation was found between nurse students’ problem-oriented coping and emotion-oriented coping and emotion processing. Conclusion: Coping strategies had significant impact on lowering level of stress. Recommendations: Develop stress management program for nursing students to reduce levels of stress and develop proper coping strategies.
Compréhension des mécanismes impliqués dans l’activité réductrice et dans les adaptations métaboliques à pH acide de Bacillus cereus : implication des thiols exofaciaux

Bacillus cereus est une bacterie a coloration de Gram positive capable de s'adapter a un grand nombre de stress tels que les bas pH ou les bas potentiels d'oxydo-reduction (Eh). Si certains des mecanismes d'adaptation de B. cereus a chacun de ces stress sont connus, les interactions entre ces deux facteurs sur la physiologie bacterienne n'ont jamais ete etudies. Nous nous sommes donc interesses a l'effet des variations de Eh sur la resistance au stress acide de B. cereus, aux adaptations metaboliques de B. cereus a pH acide et a l'interaction de cette bacterie avec son environnement redox. Les resultats obtenus ont demontre, dans un premier temps, que la survie de B. cereus au choc acide etait legerement augmentee sous atmospheres a Eh reducteurs par rapport aux atmospheres a Eh oxydants. Nous avons egalement observe une reorientation majeure du metabolisme de B. cereus expose a pH acide depuis la fermentation des acides mixtes vers la fermentation butanediolique. Cette reorientation joue vraisemblablement un role important dans la resistance au stress acide de B. cereus. Dans un second temps, nous avons etudie les interactions de B. cereus avec son environnement redox et nous avons montre l'importance des groupements thiols exofaciaux dans l'activite reductrice de cette bacterie. L'ensemble de ces resultats permettent de mieux comprendre la physiologie de B. cereus confronte a un stress acide et aux variations de Eh et ouvre la voie a de nombreuses pistes de recherches novatrices.
Purification de la chitine par hydrolyse enzymatique à partir de coproduits de crevette Penaeus vannamei. Caractérisations des produits et optimisation du procédé

The objective of this study was to optimize the extraction of chitin by acid proteolysis. The novelty of the method is based on the stabilization of pH by the balance between substrate composition and the acid solvent. This principle allows a simultaneous demineralization and deproteinization, the two main reactions associated with the purification of chitin. To evaluate the performance of this purification, the composition of the substrate and products was characterized. Different methods of quantification of chitin and proteins have been compared. As traditional assays were not satisfaying, a direct method of amino acids determination by gaz chromatography was selected to estimate the amount of protein. The estimate of chitin amount was based on indirect methods, mainly gravimetry, elemental analysis and thermogravimetric analysis (TGA). Kinetics of demineralization and deproteinization were examined to optimize the purification of chitin. Mass balances confirmed the consistency of results. The quality of chitin extracted by enzymatic or chemical techniques was compared with the degree of acetylation and depolymerization and the crystallinity index of chitin. The structure of chitin was also observed by scanning electron microscopy (SEM). Finally, compounds solubilized by enzymatic hydrolysis were identified and quantified.
Shared Fate of Meningeal Mast Cells and Sensory Neurons in Migraine

Migraine is a primary headache disorder which has complex neurogenic pathophysiological mechanisms still requiring full elucidation. The sensory nerves and meningeal mast cell couplings in the migraine target tissue are very effective interfaces between the central nervous system and the immune system. These couplings fall into three categories: intimacy, cross-talk and a shared fate. Acting as the immediate call-center of the neuroimmune system, mast cells play fundamental roles in migraine pathophysiology. Considerable evidence shows that neuroinflammation in the meninges is the key element resulting in the sensitization of trigeminal nociceptors. The successive events such as neuropeptide release, vasodilation, plasma protein extravasation, and mast cell degranulation that form the basic characteristics of the inflammation are believed to occur in this persistent pain state. In this regard, mast cells and sensory neurons represent both the target and source of the neuropeptides that play autocrine, paracrine, and neuro-endocrine roles during this inflammatory process. This review intends to contribute to a better understanding of the meningeal mast cell and sensory neuron bi-directional interactions from molecular, cellular, functional points of view. Considering the fact that mast cells play a sine qua non role in expanding the opportunities for targeted new migraine therapies, it is of crucial importance to explore these multi-faceted interactions.
Principles and Practice of Recombinant DNA Research with Yeast

INTRODUCTION Recombinant DNA research shows great promise in furthering understanding of yeast biology by making possible the analysis and manipulation of yeast genes not only in the test tube but also in yeast cells. The intent of this paper is to summarize the major features of current technology and to outline some implications for the immediate future of yeast molecular biology. There now exist simple and general methods for isolating and amplifying virtually any yeast gene, although these methods generally require an intermediate step in Escherichia coli. Powerful and exquisitely sensitive hybridization methods have been developed that allow direct physical analysis of any chromosomal region containing a gene that has been molecularly cloned. Most importantly, it is now possible to return to yeast, by transformation with DNA, cloned genes using a variety of selectable marker systems developed for this purpose. These technological advances have combined to make feasible truly molecular as well as classical genetic manipulation and analysis in yeast. The many uses to which these new tools have already been put have recently been reviewed by Olson (1981), and the results that have been obtained are to be found scattered in many of the papers in this volume. The biological problems that have been most effectively addressed by recombinant DNA technology are ones that have the structure and organization of individual genes as their central issue. Thus, the reader will find elsewhere in this volume that many signal advances in the understanding of such diverse subjects as the switching.
Aspectos comparativos de la morfología del riñón de los peces

In fish kidneys not only allow the excretion of waste but also are essential for osmoregulation and hemopoiesis. Microscopic observation differentiates two zones in the kidneys, a cranial part where there is a greater proportion of haemolymphopoietic and endocrine tissue, and other caudal whose morphology is related to the elimination of metabolic wastes and control of osmotic balance. Embryologically, the cranial portion derives from pronephros and caudal portion from mesonephros. The latter, in adult animals is called opistonefric and is formed by the excretory tissue (nephrons and collecting ducts) and lymphoid interstitial tissue. The degree of development of the nephrons differs between different groups, but in general, they are composed of the renal corpuscle and renal tubule or urine tubule, the latter can be divided into several segments. The renal tubules are reunited at collecting tubules. The main objective of this review is to describe renal morphology and its relation to environmental requirements.
Stabilization of a Cart Inverted Pendulum: Improving the Intermittent Feedback Strategy to Match the Limits of Human Performance

Stabilization of the CIP (Cart Inverted Pendulum) is an analogy to stick balancing on a finger and is an example of unstable tasks that humans face in everyday life. The difficulty of the task grows exponentially with the decrease of the length of the stick and a stick length of 32 cm is considered as a human limit even for well-trained subjects. Moreover, there is a cybernetic limit related to the delay of the multimodal sensory feedback (about 230 ms) that supports a feedback stabilization strategy. We previously demonstrated that an intermittent-feedback control paradigm, originally developed for modeling the stabilization of upright standing, can be applied with success also to the CIP system, but with values of the critical parameters far from the limiting ones (stick length 50 cm and feedback delay 100 ms). The intermittent control paradigm is based on the alternation of on-phases, driven by a proportional/derivative delayed feedback controller, and off-phases, where the feedback is switched off and the motion evolves according to the intrinsic dynamics of the CIP. In its standard formulation, the switching mechanism consists of a simple threshold operator: the feedback control is switched off if the current (delayed) state vector is closer to the stable than to the unstable manifold of the off-phase and is switched on in the opposite case. Although this simple formulation is effective for explaining upright standing as well as CIP balancing, it fails in the most challenging configuration of the CIP. In this work we propose a modification of the standard intermittent control policy that focuses on the explicit selection of switching times and is based on the phase reset of the estimated state vector at each switching time and on the simulation of an approximated internal model of CIP dynamics. We demonstrate, by simulating the modified intermittent control policy, that it can match the limits of human performance, while operating near the edge of instability.
Genetic Exchange and Homologous Recombination

After Escherichia coli, Bacillus subtilis is genetically the best-characterized bacterium. Homologous recombination has been an essential tool in the genetic analysis of B. subtilis, and in turn the availability of powerful genetic tools has facilitated investigation of the exchange of DNA-encoded information in this organism. This review summarizes the present state of knowledge concerning several modes of homologous genetic exchange and places emphasis on natural competence, about which much is known. A section discusses our understanding of the genetics and biochemistry of homologous recombination per se. Another provides an overview of the essential properties of these systems. The chapter emphasizes the newer work that concerns the regulation of competence and the nature of transformation-specific gene products. Competence is widespread in both gram-positive and gram-negative bacteria. The best-studied systems are those of Streptococcus pneumoniae, Streptococcus sanguis, Haemophilus influenzae, Neisseria gonorrhoeae, and B. subtilis. Several competence loci have been identified following the isolation of commutants. The authors propose that the five small ComG proteins assemble to form part of a cell surface-associated structure for the binding and uptake of transforming DNA. The implications of competence- linked induction of DNA repair genes and a possible mechanism for competence-linked induction of the SOS system are also discussed.
Bioremediation by alkaline protease (AkP) from edible mushroom Termitomyces clypeatus: optimization approach based on statistical design and characterization for diverse applications

BACKGROUND Bioremediation using enzymes has become an attractive approach for removing hazardous chemicals from the environment. The present study investigated the production, optimization and applications of an extracellular alkaline protease (AkP) from the edible mushroom Termitomyces clypeatus focusing on bioremediation. RESULTS For optimized enzyme production, the variables significantly influencing the protease activity were screened through the 11 factorial Plackett–Burman design. The optimum values of the selected variables and their mutual interactions were determined through response surface methodology using the Box–Behnken experimental design. Overall a 10-fold increase in enzyme activity (1830 U mL-1) was obtained which closely matched the yield predicted by the statistical model (1778.63 U mL-1) with R2 = 0.9819. AkP could efficiently dehair the goat skin, detached bird feather vanes from its shaft and reduce BOD, COD and pH of tannery waste effluent. The AkP also demonstrated bactericidal property against Staphylococcus aureus as detected by MIC and flow cytometry analyses. CONCLUSION As an ecofriendly alternative, the enzyme showed significant promise for bioremediation and industrial applications through time-saving bioprocesses. This is first report of alkaline protease from T. clypeatus or from a fungal source with wide-ranging application potential. © 2014 Society of Chemical Industry