Miracle-dz/UniFAR-FTUs huggingface dataset:
Alteration in the gene expression pattern of primary monocytes after adhesion to endothelial cells

Monocytes originate from precursors made in the bone and remain in the circulation for nearly 24 h. Much effort has been done to identify the molecules regulating transendothelial migration of monocytes during inflammatory conditions. In contrast, considerably less is known about the process of constitutive monocyte emigration although nearly 340 million monocytes leave the circulation each day in healthy individuals. Previous studies indicated that chemokines were up-regulated in monocytes cocultured with endothelial cells that induce the retraction of the latter cell type, thereby increasing vascular permeability. Thus, we hypothesized that the utilities required for efficient constitutive monocyte extravasation are generated by monocytes themselves because of adhesion to naïve endothelial cells. To test this hypothesis, cDNA microarray analysis was performed to determine the changes in the gene expression pattern of primary monocytes that have been attached to endothelial cells compared with monocytes that were held in suspension, and we were able to identify three major groups of genes. The first group includes genes such as matrix metalloproteinase 1, monocyte chemoattractant protein 1, and tissue transglutaminase 2, which are likely required for monocyte extravasation. The second group consists of genes that are expressed in phagocytes such as caveolin-1 and CD74. Finally, the third group comprises genes that are expressed in cells of endothelial tissue and cartilage including E-selectin, fibronectin-1, matrix Gla protein, and aggrecanase-2. In summary, we conclude that adhesion of peripheral blood monocytes to naïve endothelial cells has two effects: mandatory extravasation-specific genes are regulated, and the differentiation program of monocytes is initiated.
Microbe-induced lymphocyte blastogenesis enhancement after preculture

The in vitro blastogenic response of human peripheral blood mononuclear cells to Fusobacterium nucleatum and other oral microorganisms was enhanced if the peripheral blood mononuclear cells were cultured for 24 h at 37 degrees C prior to the addition of stimulant. The enhancement which occurred at optimal and supraoptimal concentrations of F. nucleatum (10 to 100 micrograms/ml) was detected after a preculture period of as little as 2 h. The blastogenic response was a result of T-cell proliferation, and enhancement occurred independently of monocytes. Suppressor activity was induced by culturing fresh lymphocytes for 24 h in the presence of supraoptimal concentrations of F. nucleatum. The enhancement phenomenon occurred independently of the prostaglandin effects on lymphocyte blastogenesis and was not abrogated by treatment with indomethacin.
Expression of recombinant human androgen receptor and its use for screening methods

This work comprises the development of new androgen receptor (AR) assays and their application. Besides other assays an androgen receptor assay was developed in microtitreplates with recombinant human AR. The assay was entirely validated, even for application with real water samples. Different classes of man-made chemicals were evaluated for their AR binding. The affinity to the AR could be demonstrated for some pesticides.
Underestimated Risk Indicator of Thromboembolic Events Platelet Hyperaggregability is Highly Prevalent in Patients With Chronic Kidney Disease : An

Background: Platelet hyperaggregation is known to be associated with arterial and venous thromboembolic events. The prevalence of platelet hyperaggregation in patients with chronic kidney disease (CKD) has not been described to date. Methods: Platelet hyperaggregation in patients with renal disease was defined by comparison of platelet aggregation patterns to non-CKD patients without thromboembolic disorders and healthy controls. Results: Among the 30 hemodialysis patients and 34 renal transplant recipients, 20 (67%) and 28 (82%) showed significantly decreased median D-epinephrine aggregation and increased 0.5 mol/L epinephrine response (65% and 54%) compared to healthy controls and non-CKD patients. In concordance to the laboratory finding of platelet hyperaggregability, renal transplant recipients showed a high rate of thromboembolic events (normal platelet aggregation: 0 events and platelet hyperaggregation: 30 events in 13 of 28 patients). Conclusions: Patients with CKD exhibit a hitherto unappreciated high prevalence of platelet hyperaggregability indicating sticky platelet syndrome. Laboratory testing of platelet hyperaggregability may supplement the assessment of thromboembolic complications in patients with CKD.
War-related stress. Addressing the stress of war and other traumatic events.

A task force on war-related stress was convened to develop strategies for prevention and treatment of psychological, psychosocial, and psychosomatic disorders associated with the Persian Gulf War and other extreme stressors facing communities in general. The task force focused on the return home, reunion, and reintegration of service personnel with their families and work. Although the Persian Gulf War was won with relative ease, negative psychological sequelae may develop in some individuals because of the stress of war, family disruption, financial difficulty, and changes in family routines. Typical stress reactions and modes of coping that are usually unsuccessful or destructive were outlined, and suggestions were made for monitoring these. In addition, guidelines for successful coping were developed. Special attention was given to children's reactions and needs. Recommendations were made for outreach and intervention on the policy, systems (e.g., schools, businesses, governmental agencies), family, and individual levels.
Treatment of childhood obesity

Purpose of reviewTo provide an overview of treatments for childhood obesity, highlighting recent advances and recommendations. Recent findingsThe three main treatment modalities are lifestyle interventions, medications, and bariatric surgery. Recent data support the short-term effectiveness of lifestyle interventions, and show that continued behavioral intervention increases the likelihood of a sustained effect for up to 2 years. New studies and regulatory decisions on medications for obesity (including orlistat, sibutramine, and metformin) are discussed. Emerging data suggest substantial weight loss after bariatric surgery in morbidly obese adolescents but also indicate adverse effects. An expert panel recently provided guidelines that alter definitions of obesity and offer a framework for obesity management. These guidelines are compared with others, and integrated recommendations presented. SummaryWhile primary prevention of childhood obesity is important, broadly effective methods to do so are not yet available. Given the large population of obese children and the risks they face, an emphasis on treatment is also critical. We suggest a staged approach, emphasizing early intervention and lifestyle changes. We also suggest limiting bariatric surgery to selected adolescents in Institutional Review Board-approved research studies. Health-policy interventions can facilitate both prevention and treatment of childhood obesity.
Functional and esthetical full mouth rehabilitation with implant supported prostheses: A case report

본 증례의 환자는 상악 전치부 보철물의 파절 및 기존에 사용하던 하악 의치의 불편감 때문에 임플란트를 이용한 고정성 보철을 원한다는 주소로 내원하였다. 상악동 거상술, 하악지 골이식과 함께 상악에 5개, 하악에 6개의 임플란트를 식립하였다. 전치부와 양쪽 구치부의 세 부분으로 나누어 임플란트 지지형 고정성 보철물을 장착하였다. 이상과 같은 과정을 통해 적절한 심미적, 기능적 결과를 얻었기에 이를 보고하고자 한다. 【This report describes the prosthetic treatment of a patient with multiple missing teeth. Installation of five fixtures on maxilla with sinus lift and six fixtures on mandible with ramal bone graft were performed. With implant supported all-ceramic with zirconia core using CAD/CAM technology and porcelain-fused-to-gold prosthesis, treatment with positive outcome which satisfies both functional and esthetical aspect was obtained. 】
CLINICAL CHARACTERISTICS OF A BATCH OF WOMEN WITH HYPERANDROGENISM BROUGHT ABOUT THE CONGENITAL ADRENAL HYPERPLASIA WITH DELAYED DEBUT

Congenital adrenal hyperplasia (CAH), non- classic form (with delayed debut) is the second cause of hyperandrogenism with a prevalence of 1,4-4%. The aim of the study was to establish the frequency of main hyperandrogenic manifestation in a group of women diagnosed with non-classic form of CAH. The study was performed on 41 patients diagnosed with non-classic form of CAH through the measurement of 17- hydroxyprogesterone and ACTH test. The following frequency was observed: hirsutism 85%, ovulatory dysfunction 56%, seborrheic syndrome 53%, alopecia 27%, and obesity 46%. hyperandrogenic manifestation. Rezumat: Hiperplazia adrenală congenitală (HAC), forma neclasică (cu debut tardiv) este considerată a doua cauză de hiperandrogenism, cu o prevalenţă de 1,4-4%. Scopul studiului a fost determinarea frecvenţei principalelor manifestări hiperandrogenice la un lot de femei diagnosticate cu forma neclasică a HAC. Studiul a fost realizat pe un număr de 41 de cazuri diagnosticate cu forma neclasică a HAC, prin dozarea 17- hidroxiprogesteronului si a testului la ACTH. A fost intâlnită următoarea frecvenţă: hirsutismul 85%, disfuncţia ovulatorie 56%, sindromul seboreic 53%, alopecia 27%, supraponderea si obezitatea 46%. Cuvinte cheie: hiperplazia adrenală congenitală, manifestări hiperandrogenice
Spinal Anesthesia with Hyperbaric Levobupivacaine and Ropivacaine for Outpatient Knee Arthroscopy: A Prospective, Randomized, Double-Blind Study

To compare unilateral spinal block produced with small doses of hyperbaric ropivacaine with that produced by 2 doses of hyperbaric levobupivacaine, we randomly allocated 91 ASA physical status I–II outpatients undergoing knee arthroscopy to receive unilateral spinal anesthesia with 7.5 mg of hyperbaric ropivacaine 0.5% (group Ropi-7.5, n = 31) or either 7.5 mg (group Levo-7.5, n = 30) or 5 mg (group Levo-5, n = 30) of hyperbaric levobupivacaine 0.5%. Spinal anesthesia was performed at the L3-4 interspace using a 25-gauge Whitacre spinal needle. The lateral decubitus position was maintained for 15 min after injection. Strictly unilateral sensory block was present in 73%, 50%, and 61% of cases in groups Ropi-7.5, Levo-7.5, and Levo-5, respectively, 30 min after injection (P = 0.40), and unilateral motor block was observed in 94%, 93%, and 83% in groups Ropi-7.5, Levo-7.5, and Levo-5, respectively (P = 0.31). One patient of group Ropi-7.5 required general anesthesia to complete surgery, and fentanyl supplementation was required in one patient of group Ropi-7.5 (3%) and one patient of group Levo-5 (3%) (P = 0.42). The median (range) time for spinal block resolution was shorter in group Ropi-7.5 (135 [126–154] min] than in group Levo-7.5 (162 [148–201] min) (P = 0.04); whereas home discharge was shorter in groups Ropi-7.5 (197 [177–218] min) and Levo-5 (197 [187–251] min) as compared with group Levo-7.5 (238 [219–277] min) (P = 0.02 and P = 0.04, respectively). We conclude that 7.5 mg of 0.5% hyperbaric ropivacaine and 5 mg of 0.5% hyperbaric levobupivacaine provide adequate spinal block for outpatient knee arthroscopy, with a faster home discharge as compared with 7.5 mg of 0.5% hyperbaric levobupivacaine.
Billman Physiology and pharmacology of the cardiac pacemaker ( b funny Q ) current

First described over a quarter of a century ago, the cardiac pacemaker bfunnyQ (If) current has been extensively characterized since, and its role in cardiac pacemaking has been thoroughly demonstrated. A similar current, termed Ih, was later described in different types of neurons, where it has a variety of functions and contributes to the control of cell excitability and plasticity. If is an inward current activated by both voltage hyperpolarization and intracellular cAMP. In the heart, as well as generating spontaneous activity, f-channels mediate autonomicdependent modulation of heart rate: h-adrenergic stimulation accelerates, and vagal stimulation slows, cardiac rate by increasing and decreasing, respectively, the intracellular cAMP concentration and, consequently, the f-channel degree of activation. Four isoforms of hyperpolarization-activated, cyclic nucleotide-gated (HCN) channels have been cloned more recently and shown to be the molecular correlates of native f-channels in the heart and h-channels in the brain. Individual HCN isoforms have kinetic and modulatory properties which differ quantitatively. A comparison of their biophysical properties with those of native pacemaker channels provides insight into the molecular basis of the pacemaker current properties and, together with immunolabelling and other detection techniques, gives information on the pattern of HCN isoform distribution in different tissues. Because of their relevance to cardiac pacemaker activity, f-channels are a natural target of drugs aimed at the pharmacological control of heart rate. Several agents developed for their ability to selectively reduce heart rate act by a specific inhibition of f-channel function; these substances have a potential for the treatment of diseases such as angina and heart failure. In the near future, devices based on the delivery of f-channels in situ, or of a cellular source of f-channels (biological pacemakers), will likely be developed for use in therapies for diseases of heart rhythm with the aim of replacing electronic pacemakers. D 2005 Elsevier Inc. All rights reserved.
Hmong Women and Reproduction . By Pranee Liamputtong Rice. Westport, Conn.: Greenwood Publishing Group, Inc., 2000. 344 pp. $65.00.

Introduction The History and the Culture The Hmong Hmong Women in Hmong Society Hmong Healers and Hmong Medicines Women and Childbearing Conception and Pregnancy Birth Nyob Nruab Hlis--Thirty Days Confinement Birth in a New Homeland Women and Childrearing Childrearing and Infant Care Beliefs about Breastmilk and Infant Feeding Practices Childhood Health and Illness Women and Reproductive Health Unsuccessful Reproduction: Infertility and Miscarriage Death in Birth: Stillbirth, Neonatal, and Maternal Death Fertility Regulation: Menstruation Regulation, Contraception, and Abortion Blood Magic: Menstruation and Menopause By Way of Conclusion--Motherhood in a New Country
Benzene-Induced Uncoupling of Naphthalene Dioxygenase Activity and Enzyme Inactivation by Production of Hydrogen Peroxide

ABSTRACT Naphthalene dioxygenase (NDO) is a multicomponent enzyme system that oxidizes naphthalene to (+)-cis-(1R,2S)-1,2-dihydroxy-1,2-dihydronaphthalene with consumption of O2 and two electrons from NAD(P)H. In the presence of benzene, NADH oxidation and O2 utilization were partially uncoupled from substrate oxidation. Approximately 40 to 50% of the consumed O2 was detected as hydrogen peroxide. The rate of benzene-dependent O2 consumption decreased with time, but it was partially increased by the addition of catalase in the course of the O2 consumption by NDO. Detailed experiments showed that the total amount of O2 consumed and the rate of benzene-induced O2 consumption increased in the presence of hydrogen peroxide-scavenging agents, and further addition of the terminal oxygenase component (ISPNAP) of NDO. Kinetic studies showed that ISPNAP was irreversibly inactivated in the reaction that contained benzene, but the inactivation was relieved to a high degree in the presence of catalase and partially relieved in the presence of 0.1 mM ferrous ion. Benzene- and naphthalene-reacted ISPNAP gave almost identical visible absorption spectra. In addition, hydrogen peroxide added at a range of 0.1 to 0.6 mM to the reaction mixtures inactivated the reduced ISPNAP containing mononuclear iron. These results show that hydrogen peroxide released during the uncoupling reaction acts both as an inhibitor of benzene-dependent O2 consumption and as an inactivator of ISPNAP. It is proposed that the irreversible inactivation of ISPNAP occurs by a Fenton-type reaction which forms a strong oxidizing agent, hydroxyl radicals (·OH), from the reaction of hydrogen peroxide with ferrous mononuclear iron at the active site. Furthermore, when [14C]benzene was used as the substrate,cis-benzene 1,2-dihydrodiol formed by NDO was detected. This result shows that NDO also couples a trace amount of benzene to both O2 consumption and NADH oxidation.
Uncertainty estimation in noise figure measurements at microwave frequencies

In this paper, a specific simulation tool is developed in order to evaluate systematic error and measurement uncertainty associated to different methodologies for noise figure measurement of microwave circuits. The tool is based on Monte-Carlo algorithms and follows the recommendations of the IEC-ISO guide to the expression of uncertainty in measurement (GUM). Representative examples are given to illustrate the capabilities of the proposed tool to assist microwave engineers in the selection of the methodology and the measurement setup best suited for their needs
Lower begging responsiveness of host versus parasitic brown-headed cowbird (Molothrus ater) nestlings is related to species identity but not to early social experience.

The survival of young brood parasites depends critically on their many adaptations to exploit hosts. Parasitic survival is particularly related to competitive superiorty for foster parental care whenever host young are not destroyed in parasitized nests.Brown-headed cowbirds (Molothrus ater) are generalist obligate parasites whose early social environments are unpredictable regarding host species and numbers of nestmates. Young avian brood parasites typically beg more intensively and loudly than foster siblings, but an untested prediction is that young parasites are also more likely to respond by begging to a wider variety of stimulus types. Avian vocalizations were used in a playback experiment to stimulate begging behavior in cowbird hosts. Compared with age-matched cowbird nestlings, hosts begged less frequently to acoustic stimuli, and lower begging responsiveness was irrespective of whether hosts had been reared in parasitized nests.
Dynamic analysis of Switched Reluctance Motor using Soft Magnetic Composite material

Soft Magnetic Composite (SMC) material is formed by surface-insulated iron powder particles, generating unique properties like magnetic and thermal isotropy, and very low eddy current loss. This paper delineates the key aspects of soft magnetic composite (SMC) material application in Switched Reluctance Motor(SRM), through dynamic magnetic characterization using Switched Reluctance Design and Simulation software (SRDaS). Three types of switched reluctance motors have been analyzed: (i) Motor with M19 steel, (ii) Motor with SMC500, and (iii) Motor with SMC1000. The comparative analysis is based on the magnetic properties and the calculated parameters of each motor model. The studied electromagnetic parameters are flux linkage, current and the electromagnetic torque.
Dose-rate effects on the cell cycle and survival of S3 HeLa and V79 cells.

The effects of continuous irradiation at different dose rates on the cell cycle and on cell survival were studied using synchronized S3 HeLa and V79 cells. The minimum dose rate necessary to stop cell division was found to be approximately 23 rad/hr for HeLa cells and 270 rad/hr for V79 cells. For dose rates that stop cell division, cells progress through G/sub 1/ and S, with a small delay in the S phase, and are blocked in G/sub 2/. Appreciable mitotic accumulation was observed for HeLa cells at dose rates which stopped cell division. By comparison, much less mitotic accumulation was observed for V79 cells over a range of dose rates from 37 to 270 rad/hr. Minimum mitotic delays for a variety of dose rates were determined for both cell lines. S3 HeLa cells are much more sensitive in this respect than V79 cells; however, it appeared that for higher dose rates the minimum mitotic delay in HeLa cells asymptotically approached a value of about 35 hr. In addition to the qualitative differences observed for the two cell lines in regard to mitotic accumulation, HeLa cells accumulated for prolonged periods in the presence of colcemid while V79 cells were blockedmore » for only a few hours, HeLa cells show a dramatic effect of redistribution of cells into sensitive phases of the cell cycle during exposure, which was reflected in the survival curves at low dose rate. More cell killing per unit dose was observed at 37 than at 74 rad/hr. « less
Kaksiulotteisesta fraktionaalisesta Brownin liikkeestä ja kuvankoodauksesta

Kuvainformaatiolle on tyypillistä, että kuvassa toisiaan lähellä olevat pisteet ovat usein tummuusasteeltaan samankaltaisia. Tutkitaan kuinka tätä dataa voidaan mallintaa stokastisella prosessilla, 2-ulotteisella fraktionaalisella Brownin liikkeellä (FBM). Mallin tarkoitus on mahdollistaa, esim. tiedonsiirron yhteydessä, kuvadatan kohtuullisen tarkka ennustaminen aiemmin välitetyn informaation perusteella, jolloin siirrettävän tiedon määrä vähenee. Toimintaperiaatteena on se, että lasketaan mallilla ennuste, sekä ennenkaikkea ennustevirheen jakauma, jonka avulla voidaan suorittaa esim. Huffman-koodaus (ks. [1]) ennustevirheelle. Pakattu tieto voidaan sitten saman jakauman avulla purkaa vastaanottopäässä. Mallin ansiosta ylimääräistä informaatiota joudutaan siirtämään vain kolmen prosessiparametrin verran. Tässä yhteydessä 2dFBM tulkitaan siten, että se liittää pinta-alaan pinnan värimäärän ja nyt kuvan ajatellan olevan jonkin tällaisen prosessin Zx realisaatio. Zx on gaussinen ja sillä on stationaariset lisäykset. Lisäksi olkoon x vastaavan vektorin määrittämä suorakaidealue, jolloin
Why am I not disabled? Making state subjects, making statistics in post--Mao China.

In this article I examine how and why disability was defined and statistically quantified by China's party-state in the late 1980s. I describe the unfolding of a particular epidemiological undertaking--China's 1987 National Sample Survey of Disabled Persons--as well as the ways the survey was an extension of what Ian Hacking has called modernity's "avalanche of numbers. " I argue that, to a large degree, what fueled and shaped the 1987 survey's codification and quantification of disability was how Chinese officials were incited to shape their own identities as they negotiated an array of social, political, and ethical forces, which were at once national and transnational in orientation.
The Quality of the Fossil Record: Implications for Evolutionary Analyses

▪ Abstract Advances in taphonomy and stratigraphy over the past two decades have dramatically improved our understanding of the causes, effects, and remedies of incompleteness in the fossil record for the study of evolution. Taphonomic research has focused on quantifying probabilities of preservation across taxonomic groups, the temporal and spatial resolution of fossil deposits, and secular changes in preservation over the course of the Phanerozoic. Stratigraphic research has elucidated systematic trends in the formation of sedimentary gaps and permanent stratigraphic records, the quantitative consequences of environmental change and variable rock accumulation rates over short and long timescales, and has benefited from greatly improved methods of correlation and absolute age determination. We provide examples of how these advances are transforming paleontologic investigations of the tempo and mode of morphologic change, phylogenetic analysis, and the environmental and temporal analysis of macroevolution.