Miracle-dz/UniFAR-FTUs huggingface dataset:
The Costs of Exhibiting Organizational Citizenship Behavior

Organizational citizenship behavior (OCB) has been associated with positive organizational outcomes and with higher managerial ratings of employee performance. However, concerns have been raised about the possible personal costs of performing such activities. This paper examines the relationship between OCB and emotional exhaustion and work-family conflict and explores the moderating role of job performance in shaping those relationships. In a time-lagged field study of customer-contact center employees the research found that one particular dimension of OCB—conscientiousness—was associated with higher emotional exhaustion and with work-family conflict. The study also revealed that conscientious employees who performed their in-role job responsibilities at a high level experienced greater emotional exhaustion and work-family conflict than conscientious employees who performed their in-role job responsibilities at a low level. Our findings suggest that organizational pressures to increase the level at which both discretionary and formal role obligations are performed can carry negative consequences for employees. © 2016 Wiley Periodicals, Inc.
Kinetics of human soluble and membrane-bound catechol O-methyltransferase: a revised mechanism and description of the thermolabile variant of the enzyme.

Human soluble (S) and membrane-bound (MB) catechol O-methyltransferase (COMT, EC 2.1.1.6) enzymes have been expressed at sufficiently high levels in Escherichia coli and in baculovirus-infected insect cells to allow kinetic characterization of the enzyme forms. The use of tight-binding inhibitors such as entacapone enabled the estimation of actual enzyme concentrations and, thereby, comparison of velocity parameters, substrate selectivity, and regioselectivity of the methylation of both enzyme forms. Kinetics of the methylation reaction of dopamine, (-)-noradrenaline, L-dopa, and 3,4-dihydroxybenzoic acid was studied in detail. Here, the catalytic number (Vmax) of S-COMT was somewhat higher than that of MB-COMT for all four substrates. The Km values varied considerably, depending on both substrate and enzyme form. S-COMT showed about 15 times higher Km values for catecholamines than MB-COMT. The distinctive difference between the enzyme forms was also the higher affinity of MB-COMT for the coenzyme S-adenosyl-L-methionine (AdoMet). The average dissociation constants Ks were 3.4 and 20.2 microM for MB-COMT and S-COMT, respectively. Comparison between the kinetic results and the atomic structure of S-COMT is presented, and a revised mechanism for the reaction cycle is discussed. Two recently published human COMT cDNA sequences differed in the position of S-COMT amino acid 108, the residue being either Val-108 [Lundström et al. (1991) DNA Cell. Biol. 10, 181-189] or Met-108 [Bertocci et al. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 1416-1420]. (ABSTRACT TRUNCATED AT 250 WORDS)
A direct approach to the estimation of the origin of oestrogens and androgens in elderly men by comparison with hormone levels in postmenopausal women.

OBJECTIVE The origin of oestrogens in men is only partly understood. From infusion studies with radioactively labelled hormones, we know that oestradiol (E2) and oestrone (E1) are either directly secreted by the testes and adrenal glands or peripherally produced from testicular or adrenal androgens. DESIGN AND METHODS We determined E2, E1, androstenedione, testosterone and dehydroepiandroster-one sulphate (DHEAS) in 292 elderly men and 367 postmenopausal women. We considered post-menopausal women as men without testes, assuming that the postmenopausal ovary is not endocrinologically active and that the testes do not contribute to circulating levels of DHEAS. Subjects were stratified by DHEAS levels to adjust for differences in DHEAS levels between sexes. For men and women separately, mean levels of E2, E1, androstenedione and testosterone were calculated per DHEAS stratum. The relative direct and indirect contributions of the testes to steroid levels in men were calculated by the formula [(C(m) - C(f))/C(m)] x 100%, in which C(m) and C(f) represent the mean concentrations of the steroid in men and women respectively. RESULTS The relative contributions (%) of the testes to hormone levels per DHEAS stratum (<2, 2-4, 4-6 and >6 micromol/l) respectively were, for E2, 72%, 60%, 52% and 44%; for E1, 54%, 47%, 35% and 34%; for androstenedione, 14%, 4%, 12% and 0%; and, for testosterone, 88%, 88%, 87% and 83%. CONCLUSIONS We conclude that in elderly men dependent on DHEAS levels, 44-72% of E2 and 34-54% of E1 originate directly or indirectly from the testes.
A mouse cerberus/Dan-related gene family.

The Xenopus cerberus gene is able to induce ectopic heads in Xenopus embryos. At the time of its identification, cerberus shared significant homology with only one other protein, the putative rat tumor suppressor protein Dan. Sequence analysis has revealed that cerberus and Dan are members of a family of predicted secreted proteins, here called the can family. The identification of a can-family member in the nematode Caenorhabditis elegans, CeCan1, suggests that this family is of ancient origin. In the mouse, there are at least five family members: Cer1, Drm, PRDC, Dan, and Dte. These genes are expressed in patterns that suggest that they may play important roles in patterning the developing embryo. Cer1 marks the anterior visceral endoderm at E6.5. Dte is expressed asymmetrically in the developing node. Dan is first seen in the head mesoderm of early head fold stage embryos and Drm is expressed in the lateral paraxial mesoderm at E8.5. The region of homology shared by these genes, here called the can domain, closely resembles the cysteine knot motif found in a number of signaling molecules, such as members of the TGFbeta superfamily. Epitope-tagged versions of Cer1 show that, unlike in TGFbeta superfamily members, the cysteine knot motif is not processed away from a proprotein. Recent experiments in Xenopus have suggested that cerberus may act as an inhibitor of BMP signaling. To examine this further, the ability of Dan, Cer1, and human DRM to attenuate Bmp4 signaling has been assessed in P19 cells using pTlx-Lux, a BMP-responsive reporter. All three genes are able to inhibit Bmp4 signaling. These data suggest that the different family members may act to modulate the action of TGFbeta family members during development.
Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts

Summary The chitin synthase that makes the primary septum during cell division in budding yeasts is an important therapeutic target with an unknown activation mechanism. We previously found that the C2-domain of the Saccharomyces cerevisiae Inn1 protein plays an essential but uncharacterised role at the cleavage site during cytokinesis. By combining a novel degron allele of INN1 with a point mutation in the C2-domain, we screened for mutations in other genes that suppress the resulting defect in cell division. In this way, we identified 22 dominant mutations of CHS2 (chitin synthase II) that map to two neighbouring sites in the catalytic domain. Chs2 in isolated cell membranes is normally nearly inactive (unless protease treatment is used to bypass inhibition); however, the dominant suppressor allele Chs2-V377I has enhanced activity in vitro. We show that Inn1 associates with Chs2 in yeast cell extracts. It also interacts in a yeast two-hybrid assay with the N-terminal 65% of Chs2, which contains the catalytic domain. In addition to compensating for mutations in the Inn1 C2-domain, the dominant CHS2 alleles suppress cytokinesis defects produced by the lack of the Cyk3 protein. Our data support a model in which the C2-domain of Inn1 acts in conjunction with Cyk3 to regulate the catalytic domain of Chs2 during cytokinesis. These findings suggest novel approaches for developing future drugs against important fungal pathogens.
Looking at the ‘sharing’ economies concept through the prism of informality

How do the ‘sharing economies’ relate to the long history of informal economic practices as understood in the social sciences? This article examines conceptions of the sharing economy in terms of its relation to scholarship on informality. By using two case studies of informal economic practices that originated in socialist-era societies and continue to the present day in modified forms, we critique the notion that sharing economies are significantly novel in form or logic, other than technologically. We draw attention to the variety of informal economy practices to discuss how they may be socially embedded or disembedded. The main focus on global technological leveraging of productivity and connectivity in sharing economy practices would suggest that many aspects are akin to disembedded forms of informality. Scholarship needs to address the ongoing disciplinary parallelism on prefixed ‘economies’—in doing so it would provide a better contextual and theoretical understanding of ‘sharing economies’.
Reduced responsiveness of cutaneous microcirculation in essential hypertension – A pilot study

Objective. Our hypothesis states that the reactivity of the cutaneous microcirculation is reduced in patients with hypertension compared with healthy subjects. The objective was to verify the hypothesis by measuring microvascular function in hypertensive patients. Design. The study was a controlled trial with two arms: 15 hypertensives and 15 normotensives were enrolled, aged 30–60 years, and in hypertensives, a diastolic blood pressure of >90 mmHg. The hypertensive patients were compared with gender‐ and age‐matched controls having a diastolic blood pressure <90 mmHg. The patients were kept on their medication. Method. The local cutaneous forearm blood flow was measured by Laser‐Doppler flowmetry. The blood flow response to local warming (44°C), to the endothelium‐dependent vasodilator acetylcholine (ACh), or to the endothelium‐independent dilators sodium nitroprusside (SNP) and calcitonin gene‐related peptide (CGRP) administered by iontophoresis were determined. Inflammatory markers and NT‐pro brain natriuretic peptide (NT‐proBNP) levels in plasma was also measured. Electrocardiograms (ECG) were evaluated and the subjects answered a lifestyle questionnaire. Results. The percentage change in vasodilator response to CGRP was significantly lower in the hypertensives compared with normotensives, 285% (95% CI 86–484) vs 764% (95% CI 366–1162) of baseline, p<0.05. The change to local warming was 2191% (95% CI 1574–2807) in normotensives vs 1384% (95% CI 852–1917) in the hypertensives, p<0.05. The vasodilator response to ACh was 1249% (95% CI 895–1602) in the normotensives and 873% (95% CI 610–1136) in the hypertensives. The vasodilator response to SNP in the normotensives was 771% (95% CI 436–1107) and 682% (95% CI 416–948) in the hypertensive group. Plasma level of NT‐proBNP was 90 ng/l (95% CI 35–145) in normotensives vs 285 ng/l (95% CI 70–499) in hypertensives (p = 0.06). The ECGs showed a tendency towards left ventricular hypertrophy (LVH) in hypertensives. Conclusion. Patients with essential hypertension had significantly reduced microvascular dilator responses to CGRP and to local warming. Also, there was a tendency towards reduced responses to ACh. This points towards a generally weaker responsiveness of the cutaneous microvessels in hypertensives and could be a contributing factor to the development of high blood pressure. Patients with essential hypertension also had a tendency of higher plasma levels of NT‐proBNP, which could be seen as an early sign of organ damage.
Design of a Bandwidth-Enhanced Polarization Rotating Frequency Selective Surface

An approach for the bandwidth enhancement of a polarization rotating frequency selective surface (FSS) based on the substrate integrated waveguide (SIW) technology is proposed in this communication. Compared with the previously reported polarization rotating FSS, the passband bandwidth of the proposed FSS is enhanced from 4.6% to 7.2% by introducing a triple-mode SIW cavity resonator as the periodic element. Meanwhile, better frequency response selectivity of filtering performance is achieved. The proposed FSS is made up of a periodic array of the novel triple-mode SIW cavity resonators with two orthogonal slots on the front and back surfaces for polarization rotation. The triple-mode SIW cavity resonator is achieved by placing a metallic via at the center of a dual-mode SIW cavity resonator. Bandwidth enhancement of the FSS is achieved by simultaneously exciting and properly merging together these three modes in the SIW cavity resonator. A prototype designed at Ka-band was fabricated and measured. Good agreement between the measurement and simulation results has been achieved.
“Self” and “Non-Self” in the Control of Phytoalexin Biosynthesis: Plant Phospholipases A2 with Alkaloid-Specific Molecular Fingerprints

Signal-transducing phospholipases A2 of two different plants are inhibited by alkaloids produced in the same but not in the other plant thus preventing continuous overproduction. The overproduction of specialized metabolites requires plants to manage the inherent burdens, including the risk of self-intoxication. We present a control mechanism that stops the expression of phytoalexin biosynthetic enzymes by blocking the antecedent signal transduction cascade. Cultured cells of Eschscholzia californica (Papaveraceae) and Catharanthus roseus (Apocynaceae) overproduce benzophenanthridine alkaloids and monoterpenoid indole alkaloids, respectively, in response to microbial elicitors. In both plants, an elicitor-responsive phospholipase A2 (PLA2) at the plasma membrane generates signal molecules that initiate the induction of biosynthetic enzymes. The final alkaloids produced in the respective plant inhibit the respective PLA, a negative feedback that prevents continuous overexpression. The selective inhibition by alkaloids from the class produced in the “self” plant could be transferred to leaves of Nicotiana benthamiana via recombinant expression of PLA2. The 3D homology model of each PLA2 displays a binding pocket that specifically accommodates alkaloids of the class produced by the same plant, but not of the other class; for example, C. roseus PLA2 only accommodates C. roseus alkaloids. The interaction energies of docked alkaloids correlate with their selective inhibition of PLA2 activity. The existence in two evolutionary distant plants of phospholipases A2 that discriminate “self-made” from “foreign” alkaloids reveals molecular fingerprints left in signal enzymes during the evolution of species-specific, cytotoxic phytoalexins.
Event-driven processing for hardware-efficient neural spike sorting

Objective. The prospect of real-time and on-node spike sorting provides a genuine opportunity to push the envelope of large-scale integrated neural recording systems. In such systems the hardware resources, power requirements and data bandwidth increase linearly with channel count. Event-based (or data-driven) processing can provide here a new efficient means for hardware implementation that is completely activity dependant. In this work, we investigate using continuous-time level-crossing sampling for efficient data representation and subsequent spike processing. Approach. (1) We first compare signals (synthetic neural datasets) encoded with this technique against conventional sampling. (2) We then show how such a representation can be directly exploited by extracting simple time domain features from the bitstream to perform neural spike sorting. (3) The proposed method is implemented in a low power FPGA platform to demonstrate its hardware viability. Main results. It is observed that considerably lower data rates are achievable when using 7 bits or less to represent the signals, whilst maintaining the signal fidelity. Results obtained using both MATLAB and reconfigurable logic hardware (FPGA) indicate that feature extraction and spike sorting accuracies can be achieved with comparable or better accuracy than reference methods whilst also requiring relatively low hardware resources. Significance. By effectively exploiting continuous-time data representation, neural signal processing can be achieved in a completely event-driven manner, reducing both the required resources (memory, complexity) and computations (operations). This will see future large-scale neural systems integrating on-node processing in real-time hardware.
Characterizations of magnetotactic bacteria conjugated versus unconjugated with carboxylate-Functionalized superparamagnetic iron oxide nanoparticles for tumor targeting purposes

Targeted drug delivery through the use of swimming microorganisms known as Magnetococcus marinus MC-1 magnetotactic bacteria (or MTB) [1] acting as nanorobots loaded with therapeutic agents has been demonstrated [2]. While the role of magnetic nanoparticles (MNPs) in medicine and more specifically drug delivery enhancement is generally known [3], this study aims to couple the previously mentioned bacteria with superparamagnetic iron oxide nanoparticles (SPIONs) via bioconjugation with antibody and without altering the bacteria cells swimming capability for drug delivery enhancement. The attachment efficiency, alignment in magnetic field and average swimming velocity in external magnetic field of the MTB-MNPs were investigated. Results indicated that MTB-MNPs could be potential agents for solid tumor targeting and cancer therapy.
An Investigation Into the Relationship of Flexibility, Power, and Strength to Club Head Speed in Male Golfers

Gordon, BS, Moir, GL, Davis, SE, Witmer, CA, and Cummings, DM. An investigation into the relationship of flexibility, power, and strength to club head speed in male golfers. J Strength Cond Res 23(5): 1606-1610, 2009-The purpose of this study was to investigate the relationship of flexibility, power, and strength to club head speed (CHS) in male golfers. Fifteen golfers (mean age ± SD: 34.3 ± 13.6 years) with a handicap of ≤8 volunteered for the study. Following a standardized warm-up, subjects proceeded to hit 5 wiffle golf balls with a 5 iron while their CHS was measured. Rotational trunk flexibility was measured on a trunk rotator machine. An index of total body rotational power was measured through a hip toss with a 3-kg medicine ball while an 8-repetition maximum (RM) on a pec deck machine was used to measure chest strength. Pearson correlations were used to assess the magnitude of the relationships between CHS and the measures of flexibility, power, and strength. Partial correlations were then run to assess the effect of handicap on the observed relationships. The only variables that were significantly correlated to CHS were chest strength (r = 0.69, p < 0.05) and total body rotational power (r = 0.54, p < 0.05). These relationships were unchanged when the effect of handicap was controlled for. The results of this study show that strength of the chest in the pec deck motion and total body rotational power significantly correlate with CHS in male golfers. This information can be used by practitioners to develop training programs and field tests for golfers.
Learning low-dimensional subspaces via sequential subspace fitting

In this paper we address the problem of learning low-dimensional subspaces using a given set of training data. To this aim, we propose an algorithm that performs by sequentially fitting a number of low-dimensional subspaces to the training data. Once we found a subset of the training data that is sufficiently near a fitted subspace, we omit these signals from the set of training signals and repeat the same procedure for the remaining signals until all training signals are assigned to a subspace. We then propose a robust version of the algorithm to address the situation in which the training signals are contaminated by additive white Gaussian noise (AWGN). Experimental results on both synthetic and real data show the promising performance of our proposed algorithm.
Adult stem cell plasticity

Observations made in the last few years support the existence of pathways, in adult humans and rodents, that allow adult stem cells to be surprisingly flexible in their differentiation repertoires. Termed plasticity, this property allows adult stem cells, assumed, until now, to be committed to generating a fixed range of progeny, to switch, when they have been relocated, to make other specialized sets of cells appropriate to their new niche. Reprogramming of some adult stem cells can occur in vivo; the stem cells normally resident in bone marrow appear particularly flexible and are able to contribute usefully to multiple recipient organs. This process produces cells with specialized structural and metabolic adaptations commensurate with their new locations. In a few examples, the degree of support is sufficient to assist or even rescue recipient mice from genetic defects. Some studies provide evidence for the expansion of the reprogrammed cells locally, but in most it remains possible that cells arrive and redifferentiate, but are no longer stem cells. Nevertheless, the fact that appropriately differentiated cells are delivered deep within organs simply by injection of bone marrow cells should make us think differently about the way that organs regenerate and repair. Migratory pathways for stem cells in adult organisms may exist that could be exploited to effect repairs using an individual's own stem cells, perhaps after gene therapy. Logical extensions of this concept are that a transplanted organ would become affected by the genetic susceptibilities of the recipient, alleles that re‐express themselves via marrow‐derived stem cells, and that plasticity after bone marrow transplantation would also transfer different phenotypes, affecting important parameters such as susceptibility to long‐term complications of diabetes, or the ability to metabolize drugs in the liver. This article reviews some of the evidence for stem cell plasticity in rodents and man. Copyright © 2002 John Wiley & Sons, Ltd.
Striatal injection of 6-hydroxydopamine induces retrograde degeneration and glial activation in the nigrostriatal pathway

PURPOSE: The effect of a highly selective 6-hydroxydopamine (6-OHDA)-induced lesion of the nigrostriatal system on the astroglial and microglial activation was analysed in adult Wistar rats after an unilateral striatal injection of the neurotoxin. METHODS: Male rats received an unilateral stereotaxical injection of the 6-OHDA in the left side of the neostriatum and were sacrificed 22 days later. Control animals received the injection of the solvent. The rotational behaviour was registered by a rotometer just before the sacrifice. Immunohistochemistry was employed for visualization of the tyrosine hydroxylase (TH) positive dopamine cells, glial fibrillary acidic protein (GFAP) immunolabeled astrocytes and OX42 immunoreactive microglia. Stereological method employing the optical disector was used to estimate the degree of the changes. RESULTS: The striatal injection of the 6-OHDA induced a massive disappearance (32% of control) of the TH immunoreactive terminals in a defined area within the striatum surrounding the injection site. A disappearance (54% of control) of dopamine cell bodies was observed in a small region of the ipsilateral pars compacta of the substantia nigra (SNc). The GFAP and OX42immunohistochemistry revealed astroglial and microglial reactions (increases in the number and size of the cells) in the ipsilateral neostriatum and SNc of the 6-OHDA injected rats. CONCLUSIONS: The striatal injection of 6-OHDA leads to retrograde degeneration as well as astroglial and microglial activation in the nigrostriatal dopamine pathway. Modulation of activated glial cells may be related to wound repair and to the trophic paracrine response in the lesioned nigrostriatal dopamine system.