Miracle-dz/UniFAR-FTUs huggingface dataset:
The Immunology of Posttransplant CMV Infection: Potential Effect of CMV Immunoglobulins on Distinct Components of the Immune Response to CMV

Abstract The immune response to cytomegalovirus (CMV) infection is highly complex, including humoral, cellular, innate, and adaptive immune responses. Detection of CMV by the innate immune system triggers production of type I IFNs and inflammatory cytokines which initiate cellular and humoral responses that are critical during the early viremic phase of CMV infection. Sustained control of CMV infection is largely accounted for by cellular immunity, involving various T-cell and B-cell subsets. In solid organ transplant patients, global suppression of innate and adaptive immunities by immunosuppressive agents limits immunological defense, including inhibition of natural killer cell activity with ongoing lowering of Ig levels and CMV-specific antibody titers. This is coupled with a short-term suppression of CMV-specific T cells, the extent and duration of which can predict risk of progression to CMV viremia. CMV immunoglobulin (CMVIG) preparations have the potential to exert immunomodulatory effects as well as providing passive immunization. Specific CMVIG antibodies and virus neutralization might be enhanced by modulation of dendritic cell activity and by a decrease in T-cell activation, effects which are of importance during the initial phase of infection. In summary, the role of CMVIG in reconstituting specific anti-CMV antibodies may be enhanced by some degree of modulation of the innate and adaptive immune responses, which could help to control some of the direct and indirect effects of CMV infection.
Functionally Distinctive Ptch Receptors Establish Multimodal Hedgehog Signaling in the Tooth Epithelial Stem Cell Niche

Continuous growth of the mouse incisor teeth is due to the life‐long maintenance of epithelial stem cells (SCs) in their niche called cervical loop (CL). Several signaling factors regulate SC maintenance and/or their differentiation to achieve organ homeostasis. Previous studies indicated that Hedgehog signaling is crucial for both the maintenance of the SCs in the niche, as well as for their differentiation. How Hedgehog signaling regulates these two opposing cellular behaviors within the confinement of the CL remains elusive. In this study, we used in vitro organ and cell cultures to pharmacologically attenuate Hedgehog signaling. We analyzed expression of various genes expressed in the SC niche to determine the effect of altered Hedgehog signaling on the cellular hierarchy within the niche. These genes include markers of SCs (Sox2 and Lgr5) and transit‐amplifying cells (P‐cadherin, Sonic Hedgehog, and Yap). Our results show that Hedgehog signaling is a critical survival factor for SCs in the niche, and that the architecture and the diversity of the SC niche are regulated by multiple Hedgehog ligands. We demonstrated the presence of an additional Hedgehog ligand, nerve‐derived Desert Hedgehog, secreted in the proximity of the CL. In addition, we provide evidence that Hedgehog receptors Ptch1 and Ptch2 elicit independent responses, which enable multimodal Hedgehog signaling to simultaneously regulate SC maintenance and differentiation. Our study indicates that the cellular hierarchy in the continuously growing incisor is a result of complex interplay of two Hedgehog ligands with functionally distinct Ptch receptors. Stem Cells 2019;37:1238–1248
Correlation between atopic diseases and tuberculin responses

Background: In recent decades, the prevalence of atopic diseases has risen steadily in developed countries. The reasons for this increase are not clear. It has been hypothesized that a reduction in infections and immunization programs may contribute to the increase in the prevalence of atopic diseases. We investigated the relationship between tuberculin response and atopic disease.
Factors Associated with Visual Impairment in Chinese American Adults: The Chinese American Eye Study

ABSTRACT Purpose: To assess associations between multiple factors comprising a conceptual model of visual impairment (VI) in a population of Chinese Americans (CAs), and identify independent VI risk factors. Methods: A population-based study of 4582 CAs aged 50 years and older residing in Monterey Park, California. A comprehensive eye examination was performed. VI was defined as best-corrected visual acuity <20/40 (US definition) in the better-seeing eye. Results: Of five independent risk factors identified, age and self-reported history of ocular disease were most strongly associated with VI. Participants 70 years and older were 10.0 times as likely to have VI compared to those in their 50s (95% confidence interval (CI) 4.0–25.0), while those with a history of ocular disease were 4.2 times as likely to have VI (95% CI 2.2–7.8). Additional risk factors included low education (OR 2.8, 95% CI 1.7–4.8), low acculturation (OR 5.9, 95% CI 2.0–17.3) and self-reported history of diabetes (OR 2.0, 95% CI 1.2–3.2). A comparison to data previously described from the Los Angeles Latino Eye Study indicated that four of the factors that predict VI risk in CAs also represent clinically relevant risk factors for VI in Latinos. Conclusions: Screening programs for individuals with advanced age and a history of ocular disease have the potential to reduce the burden of VI in CAs, as do educational programs for those with fewer years in school, a history of diabetes, and low acculturation.
Challenges for Natural Hydrogels in Tissue Engineering

Protein-based biopolymers derived from natural tissues possess a hierarchical structure in their native state. Strongly solvating, reducing and stabilizing agents, as well as heat, pressure, and enzymes are used to isolate protein-based biopolymers from their natural tissue, solubilize them in aqueous solution and convert them into injectable or preformed hydrogels for applications in tissue engineering and regenerative medicine. This review aims to highlight the need to investigate the nano-/micro-structure of hydrogels derived from the extracellular matrix proteins of natural tissues. Future work should focus on identifying the nature of secondary, tertiary, and higher order structure formation in protein-based hydrogels derived from natural tissues, quantifying their composition, and characterizing their binding pockets with cell surface receptors. These advances promise to lead to wide-spread use of protein-based hydrogels derived from natural tissues as injectable or preformed matrices for cell delivery in tissue engineering and regenerative medicine.
Collision avoidance in low thrust rendezvous guidance using flatness and positive B-splines

Collision avoidance issue in close proximity orbital rendezvous is addressed in this paper. The arising optimal control problem is solved by means of differential flatness in order to apply algebraic geometry tools. These tools based on B-splines parametrization and positive piecewise polynomial concept provide a certification of constraints satisfaction continuously in time contrary to classical collocation techniques. The non convexity derived from the avoidance constraint is overcome by using time-varying convex approximation.
Novel developments in the treatment of acute bacterial skin and skin structure infections

ABSTRACT Introduction: Acute bacterial skin and skin structure infection (ABSSSI) represents a major burden for healthcare systems. The increased prevalence of Methicillin-resistant Staphylococcus aureus, combined with the limited availability of microbiologic data when treating ABSSSI, has led to a need for more convenient, less toxic anti-MRSA agents. Recent approvals have added several agents to the antibiotic armamentarium that provide an expanded spectrum of activity and ease of administration compared to older agents. Areas covered: In this review, the authors discuss updated approaches to the management of ABSSSI. They also provide a review of recent FDA approved antibiotics and emerging investigational agents for treatment of ABSSSI. Expert opinion: Several new antibiotic agents have received FDA approval through the revised guidance on ABSSSI clinical trials with advantages of activity against MRSA and ease of administration. In theory, this may translate to reducing the utilization of healthcare resources by allowing for earlier discharge and reducing the need for outpatient parenteral therapy. While the approval of new agents offers the opportunity to improve and simplify treatment of ABSSSI, it is more important now than ever to use these agents in a responsible manner.
Barriers to and Facilitators of Weight Management in Adults Using a Meal Replacement Program That Includes Health Coaching

Purpose: The Academy of Nutrition and Dietetics supports meal replacement (MR) programs as an effective diet-related weight management strategy. While MR programs have been successful promoting initial weight loss, weight regain has been as high as 50% 1 year following MR program participation. The purpose of this article is to identify barriers to and facilitators of weight loss (WL) and weight loss maintenance (WM) among individuals participating in a MR program. Methods: Sixty-one MR program clients participated in focus groups (WL = 29, WM = 32). Barriers and facilitators were discussed until saturation of themes was reached. Focus group transcriptions were coded into themes to identify the barriers to and facilitators of weight management that emerged within each phase. Queries were run to assess frequencies of references to each theme. Results: The primary barriers within the WL phase included program products, physical activity, and social settings. WM phase participants referenced nutrition, lack of health coach knowledge, and physical activity as barriers. Personal benfits, ability to adhere to the program, and family support emerged as leading facilitators for WL phase participants. Personal benefits, health coach support, and physical activity emerged as facilitators by WM phase participants. Conclusions: Health coaches have the unique opportunity to use perceived facilitators to improve participant success, and help participants address their personal barriers in order to progress through successful, long-term weight management. Current health coaching models used in MRP should aim to identify participants’ specific barriers and develop steps to overcome them.
Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments

Effective pest management strategies for a targeted pest species must rely on accurate, reliable and reproducible estimates of population dynamics. Importance of such approaches is even more conspicuous when assessing pest’s potential to utilize other stored products. Using an experimental evolution approach, we have focused our attention on a common bean pest, the seed beetle (Acanthoscelides obtectus). We looked into the potential to invade and sustain population growth on two suboptimal host plants (chickpeas and mung beans). Such an approach simulates steps of the host-shift process in storages. By analyzing population dynamics during initial encountering with a new host plant, we detected a population drop for both novel hosts. However, transgenerational development in a novel environment resulted in a constant population growth in chickpeas, but not in mung bean populations. Reversal of chickpea selected populations to original host plant has led to a severe decrease in population parameters due to low viability of immatures, while the opposite trend was detected in mung bean populations. This paper highlights the importance of good practice in estimating population dynamics for economically important species. With special emphasis on storage pest species, we discuss how this approach can be useful for estimating invading potential of pest insects.
Iron accumulation causes impaired myogenesis correlated with MAPK signaling pathway inhibition by oxidative stress

Skeletal muscle atrophy is caused by disruption in the homeostatic balance of muscle degeneration and regeneration under various pathophysiological conditions. We have previously reported that iron accumulation induces skeletal muscle atrophy via a ubiquitin ligase–dependent pathway. However, the potential effect of iron accumulation on muscle regeneration remains unclear. To examine the effect of iron accumulation on myogenesis, we used a mouse model with cardiotoxin (CTX)‐induced muscle regeneration in vivo and C2C12 mouse myoblast cells in vitro. In mice with iron overload, the skeletal muscles exhibited increased oxidative stress and decreased expression of satellite cell markers. Following CTX‐induced muscle injury, these mice also displayed delayed muscle regeneration with a decrease in the size of regenerating myofibers, reduced expression of myoblast differentiation markers, and decreased phosphorylation of MAPK signaling pathways. In vitro, iron overload also suppressed the differentiation of C2C12 myoblast cells but the suppression could be reversed by superoxide scavenging using tempol. Excess iron inhibits myogenesis via oxidative stress, leading to an imbalance in skeletal muscle homeostasis. —Ikeda, Y. , Satoh, A. , Horinouchi, Y. , Hamano, H. , Watanabe, H. , Imao, M. , Imanishi, M. , Zamami, Y. , Takechi, K. , Izawa‐Ishizawa, Y. , Miyamoto, L. , Hirayama, T. , Nagasawa, H. , Ishizawa, K. , Aihara, K. ‐I. , Tsuchiya, K. , Tamaki, T. Iron accumulation causes impaired myogenesis correlated with MAPK signaling pathway inhibition by oxidative stress. FASEB J. 33, 9551–9564 (2019). www.fasebj. org
Enhanced Sol–Gel Route to Obtain a Highly Transparent and Conductive Aluminum-Doped Zinc Oxide Thin Film

The electrical and optical properties of sol–gel derived aluminum-doped zinc oxide thin films containing 2 at. % Al were investigated considering the modifying effects of (1) increasing the sol H2O content and (2) a thermal treatment procedure with a high-temperature approach followed by an additional heat-treatment step under a reducing atmosphere. According to the results obtained via the TG-DTA analysis, FT-IR spectroscopy, X-ray diffraction technique, and four-point probe resistivity measurements, it is argued that in the modified sample, the sol hydrolysis, decomposition of the deposited gel, and crystallization of grains result in grains of larger crystallite size in the range of 20 to 30 nm and a stronger c-axis preferred orientation with slightly less microstrain. The obtained morphology and grain-boundary characteristics result in improved conductivity considering the resistivity value below 6 mΩ·cm. A detailed investigation of the samples’ optical properties, in terms of analyzing their absorption and dispersion behaviors through UV-Vis-NIR spectroscopy, support our reasoning for the increase of the mobility, and to a lesser extent the concentration of charge carriers, while causing only a slight degradation of optical transmittance down to nearly 80%. Hence, an enhanced performance as a transparent conducting film is claimed for the modified sample by comparing the figure-of-merit values.
Prenatal Particulate Air Pollution and DNA Methylation in Newborns: An Epigenome-Wide Meta-Analysis

Background: Prenatal exposure to air pollution has been associated with childhood respiratory disease and other adverse outcomes. Epigenetics is a suggested link between exposures and health outcomes. Objectives: We aimed to investigate associations between prenatal exposure to particulate matter (PM) with diameter <10 (PM10) or <2. 5μm (PM2.5) and DNA methylation in newborns and children. Methods: We meta-analyzed associations between exposure to PM10 (n=1,949) and PM2.5 (n=1,551) at maternal home addresses during pregnancy and newborn DNA methylation assessed by Illumina Infinium HumanMethylation450K BeadChip in nine European and American studies, with replication in 688 independent newborns and look-up analyses in 2,118 older children. We used two approaches, one focusing on single cytosine-phosphate-guanine (CpG) sites and another on differentially methylated regions (DMRs). We also related PM exposures to blood mRNA expression. Results: Six CpGs were significantly associated [false discovery rate (FDR) <0.05] with prenatal PM10 and 14 with PM2.5 exposure. Two of the PM10-related CpGs mapped to FAM13A (cg00905156) and NOTCH4 (cg06849931) previously associated with lung function and asthma. Although these associations did not replicate in the smaller newborn sample, both CpGs were significant (p<0.05) in 7- to 9-y-olds. For cg06849931, however, the direction of the association was inconsistent. Concurrent PM10 exposure was associated with a significantly higher NOTCH4 expression at age 16 y. We also identified several DMRs associated with either prenatal PM10 and or PM2.5 exposure, of which two PM10-related DMRs, including H19 and MARCH11, replicated in newborns. Conclusions: Several differentially methylated CpGs and DMRs associated with prenatal PM exposure were identified in newborns, with annotation to genes previously implicated in lung-related outcomes. https://doi.org/10.1289/EHP4522
Assessing the utility of conserving evolutionary history

It is often claimed that conserving evolutionary history is more efficient than species‐based approaches for capturing the attributes of biodiversity that benefit people. This claim underpins academic analyses and recommendations about the distribution and prioritization of species and areas for conservation, but evolutionary history is rarely considered in practical conservation activities. One impediment to implementation is that arguments related to the human‐centric benefits of evolutionary history are often vague and the underlying mechanisms poorly explored. Herein we identify the arguments linking the prioritization of evolutionary history with benefits to people, and for each we explicate the purported mechanism, and evaluate its theoretical and empirical support. We find that, even after 25 years of academic research, the strength of evidence linking evolutionary history to human benefits is still fragile.
Ultra-Wideband Photonic Microwave I/Q Mixer for Zero-IF Receiver

Zero-intermediate frequency (IF) receiver features the advantages of compact structure and agile operating frequency, but faces the problems of local oscillator (LO) leakage, direct current (dc) offset, even-order distortion, and in-phase/quadrature (I/Q) imbalance. In this paper, a photonic microwave I/Q mixer for zero-IF receiver is proposed based on a single integrated polarization multiplexing Mach–Zehnder modulator (PDM-MZM). Thanks to its all-optical operation and LO power optimization, an I/Q phase imbalance below 0.9°, an isolation ratio below −30 dB, and a typical conversion gain of −2.3 dB are achieved over an ultra-wide operating frequency from 10 to 40 GHz. Combined with the balanced detection techniques, the dc offset and even-order distortions are well suppressed, and the typical third-order spurious free dynamic range is 107.9 dB <inline-formula> <tex-math notation="LaTeX">$\cdot $ </tex-math></inline-formula> Hz<inline-formula> <tex-math notation="LaTeX">$^{2/3}$ </tex-math></inline-formula>. Using the proposed photonic I/Q mixer, the vector signals with various modulation formats, carrier frequencies, and data rates (200–700 Mb/s) are directly demodulated to I/Q basebands, and the error vector magnitude and the bit rate error are measured with the power range from −25 to 15 dBm. Subharmonic vector I/Q demodulation is also experimentally demonstrated. The proposed all-optical, ultra-wideband and high-efficient microwave I/Q mixer is suitable for a zero-IF receiver and may find potential applications in electronic systems such as wideband wireless communications, frequency-hopping radar, or multiband satellite transponder.
Polyphenolic Profile and Antioxidant Capacity of Extracts from Gordonia axillaris Fruits

An ultrasonic-assisted extraction (UAE) method was adopted to extract natural antioxidants from edible Gordonia axillaris fruit. Single-factor experiments and response surface methodology were conducted to investigate the influences of five different parameters on antioxidant capacity. The optimal conditions of the UAE were 39.78% ethanol, 30.94 mL/g solvent/material ratio, 59.47 min extraction time, 40 °C temperature, and 400 W ultrasonication power. The antioxidant capacity was 525.05 ± 14.34 µmol Trolox/g DW under the optimal conditions, which was in agreement with the predicted one (531.71 µmol Trolox/g DW). Additionally, in comparison with two traditional methods (maceration and Soxhlet extraction), the established UAE method greatly improved the yield of antioxidants and significantly reduced the extraction time. Besides, nine phenolic compounds were identified and quantified in the extract of Gordonia axillaris fruits by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), including rutin, gallic acid, protocatechuic acid, epicatechin, 2-hydrocinnamic acid, p-coumaric acid, quercetin, epicatechin gallate, and ferulic acid. The richness of phenolic compounds in the Gordonia axillaris fruits indicated its potential health benefits, and its extract rich in antioxidants could be developed into functional food or nutraceuticals with the potential to prevent certain diseases induced by oxidative stress, such as cardiovascular diseases and cancers. This study also provided a way to enhance the economic values of Gordonia axillaris fruits compared to raw fruits.
A Profile of Farmers' Market Consumers and the Perceived Advantages of Produce Sold at Farmers' Markets

This study examines responses of 336 produce consumers in San Luis Obispo County, California, to compare the profile of farmersÂ’' market shoppers to those who do not shop at farmers'Â’ markets. The characteristics of produce sold in farmersÂ’' markets are compared to those sold at supermarkets to determine why consumers shop in farmersÂ’' markets. This examination of the demographic profile of farmersÂ’' market produce consumers indicates that they are more likely to be female, married, and have completed post graduate work. The age levels, income levels, and employment status are similar between farmers'Â’ market shoppers and farmers'Â’ market non-shoppers. Farmers'Â’ market shoppers indicate that cooking and family meals are important to them. Consumers indicate that quality and value are among the most important attributes when purchasing produce. Consumers perceive that farmers'Â’ market produce is fresher looking, fresher tasting, a higher-quality product, a better value for the money, more reasonably priced, more likely to be grown in their country, more likely to be locally grown, more likely to be good for the environment, and more likely to be traceable to the processor and grower when compared to supermarket produce. However, many consumers do not shop at farmersÂ’' markets due to a lack of convenience.