Miracle-dz/UniFAR-FTUs huggingface dataset:
Darstellung von retinalen Neovaskularisationen mit der optischen Kohärenztomografie-Angiografie im Vergleich mit der Fluoreszeinangiografie

Zusammenfassung Zielsetzung Vergleichende Darstellung von retinalen papillären (NVD) und nicht papillären (NVE) Neovaskularisationen in der optischen Kohärenztomografie-Angiografie (OCTA) und in der Fluoreszeinangigrafie (FA) bei Patienten mit proliferativer diabetischer Retinopathie. Material und Methoden 15 konsekutive Patienten wurden in diese Studie aufgenommen. Alle Patienten erhielten eine OCTA, wobei jeweils ein Scan von 3 × 3 mm der „Region of Interest“ mit dem ZEISS OCT Cirrus 5000 mit dem AngioPlex-Modul aufgenommen wurde. Die Größe der Neovaskularisation (NV) wurde manuell in OCTA und FA ermittelt und zwischen den beiden Messmethoden verglichen. Ergebnisse 20 Augen von 15 Patienten mit proliferativer diabetischer Retinopathie (PDR) mit einem Durchschnittsalter von 57 Jahren wurden eingeschlossen. Die mittlere Größe der NVDs betrug in der OCTA 3,44 mm2 und in der FA 3,75 mm2, die mittlere Größe der NVDs betrug in der OCTA 1,06 mm2 und in der FA 1,54 mm2. Unter Berücksichtigung einer in der FA generell größer gemessenen Fläche zeigte sich insgesamt eine gute Übereinstimmung der beiden Methoden. Schlussfolgerung Es zeigte sich eine gute Übereinstimmung für die Größe der NVs in beiden Untersuchungsmethoden. Die OCTA kann als einfache und nicht invasive Methode zur Darstellung von retinalen Neovaskularisationen die gängige Diagnostik bestätigen.
Effect of microRNA-144-5p on the proliferation, invasion and migration of human umbilical vein endothelial cells by targeting SMAD1

Atherosclerosis is a multifactorial chronic disease that is a major cause of death and injury worldwide. Apoptosis of endothelial cells (ECs) serves an important role in the occurrence and development of atherosclerosis. MicroRNAs (miRNAs) serve a key role in atherosclerosis though regulating the function of ECs. At present, the role of miRNA-144-5p (miR-144-5p) in atherosclerosis is unclear. The aim of this study was to investigate the effect of miR-144-5p on atherosclerosis in oxidized low-density lipoprotein (ox-LDL)-stimulated human umbilical vein endothelial cells (HUVECs). Results from the present study demonstrated that miR-144-5p overexpression could inhibit proliferation and induce apoptosis in HUVECs. To further study the biological function of miR-144-5p, the effects of modulating miR-144-5p expression on the invasion and migration of HUVECs were also examined. The results demonstrated that miR-144-5p upregulation suppressed HUVEC migration and invasion. TargetScan and dual luciferase reporter assay results demonstrated that SMAD1 was a direct target gene of miR-144-5p. miR-144-5p upregulation inhibited the expression of phosphorylated-SMAD1/5/8 in the SMAD pathway. In conclusion, the data indicated that miR-144-5p serves an important role in the development of atherosclerosis through regulating the function of HUVECs by targeting SMAD1.
Multivariate Analysis of Plasma Metabolites in Children with Autism Spectrum Disorder and Gastrointestinal Symptoms Before and After Microbiota Transfer Therapy

Current diagnosis of autism spectrum disorder (ASD) is based on assessment of behavioral symptoms, although there is strong evidence that ASD affects multiple organ systems including the gastrointestinal (GI) tract. This study used Fisher discriminant analysis (FDA) to evaluate plasma metabolites from 18 children with ASD and chronic GI problems (ASD + GI cohort) and 20 typically developing (TD) children without GI problems (TD − GI cohort). Using three plasma metabolites that may represent three general groups of metabolic abnormalities, it was possible to distinguish the ASD + GI cohort from the TD − GI cohort with 94% sensitivity and 100% specificity after leave-one-out cross-validation. After the ASD + GI participants underwent Microbiota Transfer Therapy with significant improvement in GI and ASD-related symptoms, their metabolic profiles shifted significantly to become more similar to the TD − GI group, indicating potential utility of this combination of plasma metabolites as a biomarker for treatment efficacy. Two of the metabolites, sarcosine and inosine 5′-monophosphate, improved greatly after treatment. The third metabolite, tyramine O-sulfate, showed no change in median value, suggesting it and correlated metabolites to be a possible target for future therapies. Since it is unclear whether the observed differences are due to metabolic abnormalities associated with ASD or with GI symptoms (or contributions from both), future studies aiming to classify ASD should feature TD participants with GI symptoms and have larger sample sizes to improve confidence in the results.
Left Ventricular Noncompaction in a Child with Turner Syndrome

Congenital heart disease (CHD) may cause a significant comorbidity in patients with Turner syndrome. The commonly reported CHD in these patients includes bicuspid aortic valve and coarctation of the aorta. Left ventricular noncompaction (LVNC) is a rare form of cardiomyopathy that has been reported in literature only three times in adult patients with Turner syndrome. We report the first case of a 6-year-old asymptomatic female with Turner syndrome who was referred for cardiac evaluation after her Turner syndrome diagnosis. Echocardiogram was suspicious for LVNC, which was confirmed on cardiac magnetic resonance imaging.
Dispersión de la Onda P en Pacientes con Exposición a Biomasa de la Comunidad la Cruz de Taratara, Municipio Sucre, Estado Falcón, Durante el Período Enero – Mayo 2017

The risk of COPD is related to the total burden of inhaled particles to which an individual is exposed during his whole life, for example indoor air pollution as result from the burning of biomass fuels for cooking. Objective: To study P wave dispersion in patients with biomass exposure of the community La Cruz de Taratara, Sucre Municipality, Falcon State, during the period January – May 2017. Methods: In this observational case-control study, with analytical non-experimental design, 30 patients with biomass exposure were included (19 women and 11 men), mean age 65.4 ± 11.7 years; control group consisted of 10 patients without biomass exposure matched by same gender and age. Electrocardiograms were performed in all patients for P wave duration manual measurement in at least 7 of the 12 leads. Results: P wave dispersion was founds to be significantly higher in patients with biomass exposure in comparison to control cases (48.33 ± 9.12 ms versus 24 ± 5.16 ms) (p: ˂ 0.05). Conclusion: Patients with biomass exposure may have a higher likelihood of atrial arrhythmias, especially paroxysmal atrial fibrillation, due to prolonged PWD.
In Vitro Schistosomicidal Activity of the Alkaloid-Rich Fraction from Ruta graveolens L. (Rutaceae) and Its Characterization by UPLC-QTOF-MS

Schistosomiasis is a neglected tropical disease that affects million people worldwide, mostly in developing countries. Ruta graveolens (Rutaceae) is a plant used in folk medicine to treat several diseases, including parasitic infections. In this study, we reported the in vitro schistosomicidal activity of the R. graveolens extract (Rg) and its active fraction (Rg-FAE). Also, the characterization of Rg-FAE by UPLC-ESI-QTOF-MS analysis and its in vitro antileishmanial activity against Leishmania braziliensis were also performed. In vitro schistosomicidal assays were assessed against adult worms of S. mansoni, while cell viability against peritoneal macrophages was measured by MTT assay. Rg (100 μg/mL) exhibited noticeable schistosomicidal activity, causing 100% mortality and decreasing motor activity of all adult male and female schistosomes, but with low activity against L. braziliensis. After chromatographic fractionation of Rg, fraction Rg-FAE was obtained, showing high activity against adult schistosomes. UPLC-ESI-QTOF-MS analysis of Rg-FAE revealed the presence of eleven alkaloids and one furanocoumarin. No significant antileishmanial activity was found for Rg, while Rg-FAE exhibited activity against L. braziliensis promastigotes. We demonstrated, for the first time, that the R. graveolens extract (Rg) and its alkaloid-rich fraction (Rg-FAE) are active against adult worms of S. mansoni, with no significant cytotoxicity on macrophages. Our findings open the route to further antiparasitic studies with the active fraction of R. graveolens and its identified compounds, especially alkaloids.
Enhanced percutaneous nerve evaluation (ePNE) of sacral nerve stimulation (SNS) using a double-blinded sham-controlled crossover test for idiopathic constipation: Quantitative and Qualitative enquiries.(The TiLTS-cc and Essence studies)

Abstract Introduction: Sacral nerve stimulation (SNS) has been increasingly utilised as a clinical treatment for chronic constipation (CC), but only a small subgroup of patients seem to respond to costly long-term treatment and the peripheral nerve evaluation test (PNE) appears less accurate than in other conditions. The lived experience of patients receiving SNS treatment, within a trial or in routine practice for any condition, is also unknown. Methods: Two systematic reviews were conducted to evaluate i) the efficacy of SNS testing, and ii) the patient experience. This was followed by a randomised sham-controlled crossover trial of a newly devised enhanced peripheral nerve evaluation (ePNE) test for SNS (the TiLTS-cc study), and a qualitative study of experiences of receiving SNS treatment for CC (the Essence study). Results: A total of 45 people were randomised, from which 29 (64%) were responders and 27 were implanted with a permanent pulse generator. At 6 month follow up there was no evidence of a difference in response between ePNE discriminate responders (60%) or ePNE indiscriminate responders (57%) (P=0.76, sensitivity 75%, specificity 15%). The study was terminated early (45/75) due to concerns regarding safety, with an infection rate of 22%. Qualitative findings, with a total of 8 people, demonstrate a constant pursuit for control over the disease, a willingness to participate in an invasive trial motivated by desire for a curative treatment, and perceptions of symptom benefit that trial definitions of benefit did not fully capture. Conclusion: The ePNE test of SNS cannot be recommended for any condition due to the high infection risk. The effect of SNS in treating CC may simply be a placebo effect, or sub-sensory SNS may be ineffective for CC. Because of patient willingness to participate in highly invasive and intrusive trials, trial design in this population should carefully monitor ongoing patient burden and patient perceptions of benefit.
The effect of high-intensity interval training (HIIT) on gene expression of the factors involved in the skeletal muscle metabolism of diabetic rats

hoc showed a significant difference between the mean expression of GLUT4 and PGC-1α of skeletal muscle in diabetic rats in different groups (P< 0.001). The changes in the expression of GLUT4 and PGC-1α of the skeletal muscle in the diabetes group were significantly lower than the control group (P=0.036). High-intensity interval training significantly caused higher the expression of GLUT4 and PGC-1α (P< 0.001). Conclusion: According to the findings of the present study, high-intensity interval training can help to improve cellular energy levels during diabetes as a result of increasing the expression of PGC-1α and GLUT4 in skeletal muscle.
Risk Factors for Gallbladder Stone Formation after Gastric Cancer Surgery

Purpose Although an increased incidence of gallbladder (GB) stone formation after gastrectomy has been reported, its etiology remains uncertain. The goal of this study was to explore the incidence of gallstone formation after gastrectomy in gastric cancer patients and investigate the risk factors therein. Materials and Methods Medical records of patients who underwent curative gastrectomy, performed by a single surgeon between August 2012 and December 2015 at the Asan Medical Center, were retrospectively reviewed. Baseline characteristics and surgical outcomes, including GB stone gallstone formation after gastrectomy, were analyzed. Results Of 561 patients included in the study, 36 presented with GB stone formation after gastrectomy for gastric cancer. The incidence of gallstone formation was 6.4%. The mean interval between gallstone formation and gastrectomy was 21.9 months. In multivariate analyses, the incidence of gallstone formation increased in patients 63 years or older, with greater than 6.2 kg weight loss in the first 6 months after the procedure, a preoperative serum total bilirubin level greater than 0.5 mg/dL, and in patients who did not receive adjuvant chemotherapy. Conclusions This study presented risk factors for GB stone formation after gastric cancer surgery, and special attention should be afforded to patients with such risk factors.
Et ve İşlenmiş Et Tüketiminin Halk Sağlığına Etkileri

Meat is an important part of human diet with strong implications in health, economic, and culture worldwide. Meat and meat products constitute important source of protein, fat, and several functional compounds. However, over consumption of meat and processed meat is the cause of public health problems such as obesity, type 2 diabetes, cardiovascular diseases and cancers. This review will examine current literature on the risks associated with red meat and processed meat consumption. The causes and consequences of effects on health of the consumption of meat and processed meat have been evaluated.
A novel cancer stem cell-based classification model for the tumorigenesis and development of colorectal cancer

Cancer is a heterogenetic disease. Although multiple hypotheses and models have been indicated, there is still no robust method to distinguish the heterogeneity of cancer. Evidence has been provided showing that cancer stem cells (CSCs) play an important role in the origin and progression of cancer. In this opinion article, based on the theory of CSCs, we propose a novel CSC-based classification model (CSCCM) to explain the origin and progression of colorectal cancer, which would explain some confusing clinical phenomena and distinguish the heterogeneity of colorectal cancer from its origin. We further suggest that this model can presently be incorporated into the cancer progression theory.
The use of fibrinogen in cardiac surgery patients-clinical and experimental studies

BACKGROUND Cardiac surgery with cardiopulmonary bypass impairs hemostasis due to hemodilution and consumption of platelets and coagulation factors, such as fibrinogen. The aim of this thesis was to study the role of fibrinogen in bleeding complications in cardiac surgery patients. METHODOLOGY Patients who underwent cardiac surgery at Sahlgrenska University Hospital from 2009 to 2017 were included in four studies. Study I assessed the importance of preoperative plasma fibrinogen concentration for excessive bleeding and the need for red blood cell transfusion in an observational study in 1954 patients. Study II was a double-blinded placebo-controlled study where 48 patients were randomized to prophylactic administration of fibrinogen concentrate or placebo. Primary endpoint was postoperative bleeding volume. Study III was an observational study in 5408 patients that assessed if patients who had received perioperative fibrinogen concentrate due to bleeding had a higher risk of thromboembolic complications or death. Study IV was an in vitro study where fibrinogen concentrate was added to blood samples from 15 patients to investigate if concomitant tranexamic acid and fibrinogen administration has additional effects on clot formation compared to fibrinogen alone. RESULTS The preoperative plasma fibrinogen concentration correlated inversely to increased postoperative bleeding but not to RBC transfusion. Prophylactic infusion of 2 g fibrinogen concentrate did not reduce postoperative bleeding volume. Patients who received fibrinogen concentrate due to perioperative bleeding did not have a higher risk of thromboembolic complications or death during the first year after surgery. The combination of tranexamic acid and fibrinogen did not have additional effects on platelet-independent clotting time or clot firmness than
Efficacy of Myofascial Release

Date Presented 04/04/19 This session will inform practitioners about recent evidence surrounding the efficacy of myofascial release in the treatment of adults with upper-extremity orthopedic or peripheral nerve conditions. Content will address implications and limitations. Primary Author and Speaker: Sheryl Zylstra Additional Authors and Speakers: Tiffany Farley, Michelle Chait, Erin Eaton, George Tomlin
Effects of the systemic administration of omega -3 polyunsaturated fatty acid on experimental periodontitis (immunological analysis for rat’s serum)

Objective: The present study was aimed to investigate and evaluate the effects of eicosapentaenoic acid (EPA) on ligature induced periodontitis in rats through the biochemical analysis for ratâ€™s serum level of CRP, ALP and MDA. Methods: Periodontitis was induced for the studied animals by ligation around the upper central incisor. Twenty five animals were used as a control and gavaged by water only, and one hundred animals with the induced periodontitis were divided into four equal groups according to the treatment used which was started at time of ligation removal: Water (P/W), scaling/root planing (P/SRP), 60mg/kg EPA(P/EPA), and scaling/root planing together with EPA(P/SRP+EPA). Blood was taken by cardiac puncture, after three hours, 24 hours, three days, one week and two weeks for biochemical analysis. Results: The P/SRP+EPA treatment group showed significant decrease (p<0.05) in serum CRP, significant increase in serum ALP, significant decrease in serum MDA after one week, three days, and three hours respectively in comparison with the P/W treatment group. Conclusion: The treatment by SRP with 60mg/kg EPA is better than each one alone and can be used for treatment of periodontitis.
Improving Knowledge Transfer by Using a Summative Patient Handout for Cystoscopy.

INTRODUCTION Despite cystoscopy being among the most commonly performed urological procedures, there is a paucity of information on patient comprehension and retention of cystoscopy outcomes. A quality assessment performed at our center revealed low rates of patient understanding in our current care model. Therefore, we assessed patient comprehension of outpatient cystoscopy outcomes before and after the implementation of a formal written communication tool in the form of a summative patient handout to improve physician-patient communication. METHODS Consecutive patients reporting for cystoscopy at a Canadian tertiary care center were assessed with postprocedure questionnaires before and after the implementation of a summative patient handout to facilitate the communication of results and followup plans. Comparisons were made with the Chi-squared test (p <0.05). RESULTS A total of 650 questionnaires were analyzed (500 baseline and 150 postsummative patient handout). Of the respondents 448 (69%) were male and the mean age was 66 years old (total range 21 to 94 years) with 32% being under 60 years old. The proportion of patients who felt their results were discussed with them after implementation of the summative patient handout improved (94% vs 87%, p=0.02). Similarly, we improved concordance of patient reported and urologist reported cystoscopy results (75% vs 56%, p <0.001). Finally, we improved patient identification of their postcystoscopy followup plan (80% vs 51%, p <0.001). CONCLUSIONS The implementation of a summative patient handout after local cystoscopy improved patient understanding of their procedural results and postcystoscopy followup plans.
Estado nutricional del paciente trasplantado renal. Asociación con la función del injerto renal, la insulinorresistencia y las complicaciones metabólicas

Se exploraron las asociaciones entre el estado nutricional de 60 pacientes trasplantados renales y la vitalidad del injerto renal, la presencia de insulinorresistencia y la ocurrencia de complicaciones metabolicas. El 21.8% de los pacientes estaba desnutrido, mientras que la obesidad afecto el 26.6%. Los pacientes desnutridos mostraron los valores menores de Albumina, Colesterol y Hemoglobina, junto con las cifras mayores de Trigliceridos. La desnutricion se asocio con una funcion disminuida del injerto renal: el filtrado glomerular observado en los desnutridos fue de 26.4 ± 12.1 L.minuto -1 , y la Creatinina serica fue de 304.0 ± 62.3 mmol.L -1 . En todos los trasplantados se constato la presencia de insulinorresistencia tras la observacion de niveles sericos elevados de insulina 2 horas despues de una sobrecarga de glucosa y valores altos del indice HOMA-R. Las complicaciones metabolicas se concentraron en los trasplantados obesos: el 100.0% de ellos presento hipertension arterial, el 75.0% mostro hipercolesterolemia; y el 37.5% diabetes post-trasplante. Por el contrario, la hipertrigliceridemia afecto primordialmente al 53.8% de los trasplantados renales. El trasplantado renal puede mostrar fenotipos nutricionales polares, en relacion con factores patogenicos como la funcion disminuida del injerto renal y la presencia de insulinorresistencia. Estos fenotipos nutricionales pueden contribuir a la aparicion de fenomenos metabolicos, como la hipertension arterial, las dislipidemias y la diabetes post trasplante. El estado nutricional del trasplantado renal debe ser seguido sistematica y regularmente por el equipo basico de trabajo, a fin de detectar y corregir inmediatamente los trastornos nutricionales que se presenten, debido a la implicacion metabolica de los mismos.
Demonstrating the Performance of Accelerated Failure Time Model over Cox-PH Model of Survival Data Analysis with Application to HIV-Infected Patients under HAART

Background: Human Immunodeficiency Virus (HIV) is a virus that kills CD4 cells. These CD4 cells are white blood cells that fight infection. CD4 count is like a snapshot of how well our immune system is functioning. Studying the way of CD4+ count over time provides an insight to the disease evolution. Methods: This study was considering the data of HIV/AIDS patients who were undergoing Antiretroviral Therapy in the ART clinic of Menellik II Referral Hospital, Addis Ababa, Ethiopia, during the period 1st January 2014 to 31st December 2017. The data was analyzed in separate survival models i.e non parametric, semi parametric (Cox PH) and parametric survival model (AFT models). For the purpose of model diagnosis cox-snail residual analysis were incorporated. Results: For separate survival model log-logistic model is more appropriate for the survival data than other parametric models. Therefore; functional status and regimen class are significant covariates in determining the hazard function patients. In the Log-logistic model, among the covariates we have included in the survival model: functional status (working subgroup) and regimen class (all subgroup) were significant at 5% level of significance. But, sex, age, baseCD4, marital status and WHO-clinical stage are not significance at 5% significance level. Using cox-snail residual shows proportionality not satisfied for these WHO stage, regimen class and marital status. Conclusions: Log rank and Wilcoxon tests showed that the significant difference in survival situation among the categorical variables selected for this study sex, marital status, functional status, WHO-clinical stages and regimen class subgroups. But, there was no significant difference in the time-to-event between subgroups of sex, Marital Status and WHO clinical Stage, while, Regimen Class and Functional Status there was a significant difference in the time-to-event between subgroups.