Miracle-dz/UniFAR-FTUs huggingface dataset:
Dissipation Analysis Methods and Q-Enhancement Strategies in Piezoelectric MEMS Laterally Vibrating Resonators: A Review

Over the last two decades, piezoelectric resonant sensors based on micro-electromechanical systems (MEMS) technologies have been extensively studied as such sensors offer several unique benefits, such as small form factor, high sensitivity, low noise performance and fabrication compatibility with mainstream integrated circuit technologies. One key challenge for piezoelectric MEMS resonant sensors is enhancing their quality factors (Qs) to improve the resolution of these resonant sensors. Apart from sensing applications, large values of Qs are also demanded when using piezoelectric MEMS resonators to build high-frequency oscillators and radio frequency (RF) filters due to the fact that high-Q MEMS resonators favor lowering close-to-carrier phase noise in oscillators and sharpening roll-off characteristics in RF filters. Pursuant to boosting Q, it is essential to elucidate the dominant dissipation mechanisms that set the Q of the resonator. Based upon these insights on dissipation, Q-enhancement strategies can then be designed to target and suppress the identified dominant losses. This paper provides a comprehensive review of the substantial progress that has been made during the last two decades for dissipation analysis methods and Q-enhancement strategies of piezoelectric MEMS laterally vibrating resonators.
Effect of maternal preconceptional and pregnancy micronutrient interventions on children's DNA methylation: Findings from the EMPHASIS study

ABSTRACT Background Maternal nutrition in pregnancy has been linked to offspring health in early and later life, with changes to DNA methylation (DNAm) proposed as a mediating mechanism. Objective We investigated intervention-associated DNAm changes in children whose mothers participated in 2 randomized controlled trials of micronutrient supplementation before and during pregnancy, as part of the EMPHASIS (Epigenetic Mechanisms linking Preconceptional nutrition and Health Assessed in India and sub-Saharan Africa) study (ISRCTN14266771). Design We conducted epigenome-wide association studies with blood samples from Indian (n = 698) and Gambian (n = 293) children using the Illumina EPIC array and a targeted study of selected loci not on the array. The Indian micronutrient intervention was food based, whereas the Gambian intervention was a micronutrient tablet. Results We identified 6 differentially methylated CpGs in Gambians [2.5–5.0% reduction in intervention group, all false discovery rate (FDR) <5%], the majority mapping to ESM1, which also represented a strong signal in regional analysis. One CpG passed FDR <5% in the Indian cohort, but overall effect sizes were small (<1%) and did not have the characteristics of a robust signature. We also found strong evidence for enrichment of metastable epialleles among subthreshold signals in the Gambian analysis. This supports the notion that multiple methylation loci are influenced by micronutrient supplementation in the early embryo. Conclusions Maternal preconceptional and pregnancy micronutrient supplementation may alter DNAm in children measured at 7–9 y. Multiple factors, including differences between the nature of the intervention, participants, and settings, are likely to have contributed to the lack of replication in the Indian cohort. Potential links to phenotypic outcomes will be explored in the next stage of the EMPHASIS study.
The psychosocial risk of being 'extremely vulnerable' during Covid-19 and the role of behaviour activation.

Many individuals shielding from Covid-19 because of extreme clinical vulnerability are reporting worsening mental health (ONS, 2020) and may need assistance to change their shielding behaviour to regain their social connections. Isolation is strongly linked to depression and anxiety, which provoke further social withdrawal (Santini et al, 2020), creating a downward spiral of low mood, lack of motivation, anxiety, and further isolation through avoidance behaviour.
Fabrication of ZnO/Carbon Quantum Dots Composite Sensor for Detecting NO Gas

ZnO and carbon quantum dots (CQDs) were synthesized by a hydrothermal method, and CQDs were doped into ZnO by a grinding method to fabricate a ZnO/CQDs composite. The X-ray diffraction and the scanning electron microscope revealed that the as-prepared ZnO has a structure of wurtzite hexagonal ZnO and a morphology of a flower-like microsphere which can provide more surface areas to adsorbed gases. The ZnO/CQDs composite has a higher gas sensitivity response to NO gas than ZnO microspheres. A gas sensitivity test of the ZnO/CQDs composite showed that the sensor had a high NO response (238 for 100 ppm NO) and NO selectivity. The detection limit of the ZnO/CQDs composite to NO was 100 ppb and the response and recovery times were 34 and 36 s, respectively. The active functional group provided by CQDs has a significant effect on NO gas sensitivity, and the gas sensitivity mechanism of the ZnO/CQDs composite is discussed.
Ketamine for Prehospital Pain Management Does Not Prolong Emergency Department Length of Stay

Abstract Background Ketamine is gaining acceptance as an agent for prehospital pain control, but the associated risks of agitation, hallucinations and sedation have raised concern about its potential to prolong emergency department (ED) length of stay (LOS). This study compared ED LOS among EMS patients who received prehospital ketamine, fentanyl or morphine specifically for pain control. We hypothesized ED LOS would not differ between patients receiving the three medications. Methods This retrospective observational study utilized the 2018 ESO Research Database, which includes more than 7.5 million EMS events attended by more than 1,200 agencies. Inclusion criteria were a 9-1-1 scene response; age ≥ 18 years; a recorded pain score greater than 4; an initial complaint or use of a treatment protocol indicating a painful condition; prehospital administration of ketamine, fentanyl or morphine; and ED LOS data available. Patients were excluded if they received a combination of the medications, or if there were indications that medication administration could have been for airway management (i.e., altered mental status, head injury, respiratory distress/depression) or agitation control (e.g., behavioral complaints). Kruskal-Wallis test was used to compare ED LOS among patients receiving each of the three medications. Post-hoc evaluations of between-group differences were conducted using Wilcoxon Rank Sum test and a Bonferroni-corrected alpha value of 0.017. Results Of 9,548 patients who met the inclusion criteria, 119 received ketamine, 1,359 received morphine, and 8,070 received fentanyl. Patient and event characteristics did not significantly differ between the three groups. Median (IQR) ED LOS was 3.5 (2.5–6.1) hours for patients who received ketamine, 4.0 (2.7–6.1) hours for patients who received morphine, and 3.7 (2.6–5.4) hours for patients who received fentanyl (p = 0.002). In post-hoc pairwise comparisons, patients who received morphine had significantly longer ED LOS than patients who received fentanyl (p < 0.001); there was no significant difference in ED LOS for patients who received ketamine vs. morphine (p = 0.161) or for patients who received ketamine vs. fentanyl (p = 0.809). Conclusion ED LOS is not longer for patients who receive prehospital ketamine, versus morphine or fentanyl, for management of isolated painful non-cardiorespiratory conditions.
Simple tests of cardiorespiratory fitness in a pediatric population

A progressive, treadmill-based VO2max is the gold standard of cardiorespiratory fitness determination but is rarely used in pediatric clinics due to time requirements and cost. Simpler and shorter fitness tests such as the Squat Test or Step Test may be feasible and clinically useful alternatives. However, performance comparisons of these tests to treadmill VO2max tests are lacking. The primary aim of this cross-sectional study was to assess the correlation between Squat and Step Test scores and VO2max in a pediatric population. As secondary outcomes, we calculated correlations between Rated Perceived Exertion Scale (RPE) scores, NIH PROMIS Physical Activity scores, and BMI z-score with VO2max, and we also evaluated the ability of each fitness test to discriminate low and high-risk patients based on the FITNESSGram. Forty children aged 10–17 completed these simple cardiorespiratory fitness tests. Statistically significant correlations were observed between VO2max and the Step Test (r = -0.549) and Squat Test (r = -0.429) scores, as well as participant BMI z-score (r = -0.458). RPE and PROMIS scores were not observed to be correlated with VO2max. Area Under the Receiver Operator Curve was relatively high for BMI z-scores and the Step Test (AUC = 0.813, 0.713 respectively), and lower for the Squat Test (AUC = 0.610) in discriminating risk according to FITNESSGram Scores. In this sample, the Step Test performed best overall. These tests were safe, feasible, and may add great value in assessing cardiorespiratory fitness in a clinical setting.
Activation of cholinergic anti-inflammatory pathway involved in therapeutic actions of α-mangostin on lipopolysaccharide-induced acute lung injury in rats

Introduction: Alpha-mangostin (MAN) possesses a wide variety of pharmacological effects. In this study, we investigated its effect on cholinergic anti-inflammatory pathway (CAP), and tested if CAP regulation was involved in the therapeutic action on acute lung injury (ALI). Methods: Male Sprague Dawley rats were pre-treated with MAN (40 mg/kg) for 3 days and ALI was induced with an intraperitoneal injection of lipopolysaccharide (LPS). Certain rats received monolateral vagotomy or sham surgery. The effects on inflammatory reactions and relevant pathways in ALI rats or LPS pre-treated RAW 264.7 cells were investigated by histological, immunohistochemical, immunoblotting, RT-qPCR, and immunofluorescence assays, while levels of proinflammatory cytokines, acetylcholine (Ach) and the enzymatic activity of acetylcholinesterase (AchE) were determined by corresponding quantitative kits. Results: Oral administration of MAN reduced the severity of ALI, while vagotomy surgery antagonized this effect. MAN restored the decline in α7 nicotinic acetylcholine receptor (α7nAchR) in the lungs of ALI rats, and promoted the expression of α7nAchR and choline acetyltransferase (CHAT) in RAW 264.7 cells. Although AchE expression was barely affected by MAN at 5 μg/ml, its catalytic activity was reduced by almost 95%. Extracellular rather than intracellular Ach was notably raised shortly after MAN treatment. Furthermore, MAN at 5 μg/ml effectively inhibited LPS-induced increase in phosphorylation and nucleus translocation of p65 subunit, and secretion of TNF-α and IL-1β, which was then offset by methyllycaconitine citrate hydrate. Conclusion: MAN activated CAP by increasing peripheral Ach and up-regulating α7nAchR expression, which eventually led to NF-κB inhibition and remission of acute inflammations.
Photosynthesis in two neotropical palm species

The photosynthetic responses of the palms Socratea durissima (Oerst.) H. Wendl. and Scheelea zonensis Bailey, two species with contrasting growth forms, were studied. Light-saturated rates in the laboratory were 3 38 and 5 74 Lmol m-2s-1 for Socratea and Scheelea respectively, grown at 25% full sunlight; the respective rates were 1-16 and 1-61 Lmol m-2s-1 when grown at 1-5% of full sunlight. In the field, leaf nitrogen declines more rapidly with age in Socratea than in Scheelea. Data on stomatal density indicate that maximum conductance contributes to between-species and between-treatment differences in light-saturated photosynthesis in the laboratory, while declining leaf nitrogen with leaf age may reduce photosynthesis more in Socratea than in Scheelea. The shade-adapted photosynthetic responses of these two species allows them to grow as seedlings in the shade of the tropical forest understorey. Scheelea shows greater differences in photosynthetic responses when grown under high vs low light than Socratea does; this difference between the two species suggests that Scheelea is more suited to exploiting treefall gaps than Socratea is. The characterization, based on physiological responses, of Scheelea as more of a gap species than Socratea is consistent with interpretations based on plant architecture and with field observations of the population biology of these two species. Key-words: Photosynthesis, palms, tropical, leaf age, plant architecture, Socratea durissima, Scheelea zonensis
Arthrobacter mobilis sp. nov., a novel actinobacterium isolated from Cholistan desert soil.

A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity). The peptidoglycan was of the A3α type and the whole-cell sugar profile was found to contain galactose. The major menaquinone was MK-9(H2). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids. The major fatty acids identified were anteiso-C15 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 68.69 mol%. The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively. On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp. nov. is proposed. The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).
Flaviflexus ciconiae sp. nov., isolated from the faeces of the oriental stork, Ciconia boyciana.

A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea. Optimal growth of strain H23T48T was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl. 16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively. Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids. The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids. The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine. The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %. Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus, for which the name Flaviflexus ciconiae sp. nov. is proposed. The type strain is H23T48T (=KCTC 49253T=JCM 33282T).
Nutritional, Microbial, and Sensory Evaluation of Complementary Foods Made from Blends of Orange-Fleshed Sweet Potato and Edible Insects

Improved formulations of complementary foods (CFs) with animal-source foods (ASFs) is an important strategy to improve infant and young child feeding (IYCF). However, ASFs are expensive in many food-insecure settings where edible insects abound. CFs were developed from flours of orange-fleshed sweet potato (OFSP) and cricket (OFSCri) or palm weevil larvae (OFSPal) or soybean (OFSSoy) in the ratio 7:3. Nutritional and microbial quality of the novel CFs were determined and compared with Weanimix (recommended maize-peanut-soybean blend). Sensory evaluation of porridges was rated on a five-point hedonic scale among 170 Ghanaian mothers. OFSCri (20.33 ± 0.58 g/100 g) and Weanimix (16.08 ± 0.13 g/100 g) met the protein requirement of 15 g/100 g from CFs. Although Fe content was significantly higher for OFSCri (1.17 ± 0.03 mg/100 g), none of the CFs met the recommended levels for Fe. All the CFs were free from Salmonella, and aerobic plate count was significantly below permissible levels. All the CFs were ranked above the minimum threshold (hedonic scale = 3; neither like nor dislike) of likeness for the sensory attributes. Crickets and palm weevil larvae can be blended with OFSP and could be sustainable, culturally appropriate alternative ASFs for IYCF, but long-term studies are needed to evaluate their efficacy.
COMPUESTOS ORGÁNICOS VOLÁTILES EMITIDOS POR Phaseolus vulgaris CONTRIBUYEN EN LA DEFENSA HACIA ANTRACNOSIS

En el presente trabajo, se utilizaron cuatro variedades de frijol comun (P. vulgaris) clasificadas de acuerdo a su nivel de resistencia hacia Colletotrichum lindemuthianum (agente causal de la antracnosis); identificando un cultivar resistente y una linea susceptible. Posteriormente, se analizaron e identificaron los compuestos organicos volatiles (COVs) emitidos por las plantas resistentes y susceptibles al fitopatogeno. De los perfiles obtenidos fueron seleccionados COVs exclusivos de las plantas resistentes, asi como los COVs compartidos por ambas lineas. Los volatiles seleccionados fueron probados para evaluar su actividad antifungica contra C. lindemuthianum. Se encontro que los compuestos presentes en ambas lineas (cis-hexenil-acetato, farnesol y cariofileno) no presentaron ningun efecto. Por otro lado, los volatiles nonanal, terpineol y trans-2-decanal tuvieron un efecto negativo sobre el crecimiento micelial del patogeno estudiado. Finalmente, concluimos que los COVs pueden actuar como agentes antifungicos y mediar la resistencia contra el hongo C. lindemuthianum.
Principles for Management of Aquatic-Breeding Amphibians

Coordinated efforts by ecologists and natural resource managers are necessary to balance the conservation of biological diversity with the potential for sustained economic development. Because some amphibians have suffered world-wide declines during the last 20 years, it is important to consider biologically based management strategies that will preserve local and regional populations. This paper provides a brief overview of potential threats to local and regional populations, the state of knowledge on population and landscape processes, and the critical elements needed for an effective management plan for amphibians. Local population dynamics and ecological connectivity of amphibian metapopulations must be considered in effective management plans. There are 3 critical factors to consider in a management plan (1) the number or density of individuals dispersing from individual wetlands, (2) the diversity of wetlands with regard to hydroperiod, and (3) the probability of dispersal among adjacent wetlands or the rescue and recolonization of local populations. Wetland losses reduce the total number of sites where pond-breeding amphibians can reproduce and recruit juveniles into the breeding population. Loss of small, temporary wetlands (<4.0 ha) may be especially harmful to amphibians because of their abundance and high species diversity. Alteration of wetlands, particularly hydrologic cycles, can severely impair completion of larval metamorphosis through either early pond drying (if hydroperiod is shortened) or through increased predation (if hydroperiod is lengthened or connections made with fish-infested lakes, rivers, or canals). Wetland loss also increases the distance between neigh-boring wetlands that is critical to metapopulation source-sink processes. Reduction in wetland density reduces the probablity that populations will be rescued from extinction by nearby source populations. Local populations cannot be considered independent of source-sink processes that connect wetlands at the landscape or regional level. Further, the fragmentation of natural habitats from timber harvesting, agriculture, roads, drainage canals, or urban development impedes or prevents dispersal and decreases the probability of wetland recolonization. If our goal is to maintain or enhance present levels of amphibian diversity, then resource managers must incorporate critical elements into plans that protect population and landscape processes thereby maintaining viable populations and communities of amphibians.
An Experimentally Defined Hypoxia Gene Signature in Glioblastoma and Its Modulation by Metformin

Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor, characterized by a high degree of intertumoral heterogeneity. However, a common feature of the GBM microenvironment is hypoxia, which can promote radio- and chemotherapy resistance, immunosuppression, angiogenesis, and stemness. We experimentally defined common GBM adaptations to physiologically relevant oxygen gradients, and we assessed their modulation by the metabolic drug metformin. We directly exposed human GBM cell lines to hypoxia (1% O2) and to physioxia (5% O2). We then performed transcriptional profiling and compared our in vitro findings to predicted hypoxic areas in vivo using in silico analyses. We observed a heterogenous hypoxia response, but also a common gene signature that was induced by a physiologically relevant change in oxygenation from 5% O2 to 1% O2. In silico analyses showed that this hypoxia signature was highly correlated with a perinecrotic localization in GBM tumors, expression of certain glycolytic and immune-related genes, and poor prognosis of GBM patients. Metformin treatment of GBM cell lines under hypoxia and physioxia reduced viable cell number, oxygen consumption rate, and partially reversed the hypoxia gene signature, supporting further exploration of targeting tumor metabolism as a treatment component for hypoxic GBM.
Neuron‐specific deletion of CuZnSOD leads to an advanced sarcopenic phenotype in older mice

Age‐associated loss of muscle mass and function (sarcopenia) has a profound effect on the quality of life in the elderly. Our previous studies show that CuZnSOD deletion in mice (Sod1−/− mice) recapitulates sarcopenia phenotypes, including elevated oxidative stress and accelerated muscle atrophy, weakness, and disruption of neuromuscular junctions (NMJs). To determine whether deletion of Sod1 initiated in neurons in adult mice is sufficient to induce muscle atrophy, we treated young (2‐ to 4‐month‐old) Sod1flox/SlickHCre mice with tamoxifen to generate i‐mn‐Sod1KO mice. CuZnSOD protein was 40‐50% lower in neuronal tissue in i‐mn‐Sod1KO mice. Motor neuron number in ventral spinal cord was reduced 28% at 10 months and more than 50% in 18‐ to 22‐month‐old i‐mn‐Sod1KO mice. By 24 months, 22% of NMJs in i‐mn‐Sod1KO mice displayed a complete lack of innervation and deficits in specific force that are partially reversed by direct muscle stimulation, supporting the loss of NMJ structure and function. Muscle mass was significantly reduced by 16 months of age and further decreased at 24 months of age. Overall, our findings show that neuronal‐specific deletion of CuZnSOD is sufficient to cause motor neuron loss in young mice, but that NMJ disruption, muscle atrophy, and weakness are not evident until past middle age. These results suggest that loss of innervation is critical but may not be sufficient until the muscle reaches a threshold beyond which it cannot compensate for neuronal loss or rescue additional fibers past the maximum size of the motor unit.