Miracle-dz/UniFAR-FTUs huggingface dataset:
Molecular Characterization of a Carbon Transporter in Plastids from Heterotrophic Tissues: The Glucose 6-Phosphate/Phosphate Antiporter

Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy. Within plastids, carbon can be used in the biosynthesis of starch or as a substrate for the oxidative pentose phosphate pathway, for example. We have used maize endosperm to purify a plastidic glucose 6-phosphate/phosphate translocator (GPT). The corresponding cDNA was isolated from maize endosperm as well as from tissues of pea roots and potato tubers. Analysis of the primary sequences of the cDNAs revealed that the GPT proteins have a high degree of identity with each other but share only ~38% identical amino acids with members of both the triose phosphate/phosphate translocator (TPT) and the phosphoenolpyruvate/phosphate translocator (PPT) families. Thus, the GPTs represent a third group of plastidic phosphate antiporters. All three classes of phosphate translocator genes show differential patterns of expression. Whereas the TPT gene is predominantly present in tissues that perform photosynthetic carbon metabolism and the PPT gene appears to be ubiquitously expressed, the expression of the GPT gene is mainly restricted to heterotrophic tissues. Expression of the coding region of the GPT in transformed yeast cells and subsequent transport experiments with the purified protein demonstrated that the GPT protein mediates a 1:1 exchange of glucose 6-phosphate mainly with inorganic phosphate and triose phosphates. Glucose 6-phosphate imported via the GPT can thus be used either for starch biosynthesis, during which process inorganic phosphate is released, or as a substrate for the oxidative pentose phosphate pathway, yielding triose phosphates.
Delayed enhanced nitric oxide-mediated coronary vasodilation following brief ischemia and prolonged reperfusion in conscious dogs.

The goal of this study was to determine both the early and delayed effects of a brief (10-minute) coronary artery occlusion (CAO) and prolonged (5-day) reperfusion (CAR) on coronary endothelial function. Fourteen mongrel dogs were chronically instrumented to measure aortic and left ventricular pressures, wall thickness, and left circumflex coronary blood flow (CBF). Before CAO and during CAR, coronary vascular reactivity was investigated by 15-second CAO and subsequent reactive hyperemia (RH) and by the selective intracoronary infusion of acetylcholine (ACh, 10 micrograms/min) and bradykinin (BK, 2.5 micrograms/min), endothelium-dependent vasodilators, and sodium nitroprusside (SNP, 40 micrograms/min), an endothelium-independent vasodilator. CBF responses to ACh and BK began to increase after 6 hours of CAR, reached a peak after 1 to 2 days of CAR, and then subsided over the subsequent 4 days. After 1 day of CAR, compared with before CAO, enhanced CBF responses (P < .05), associated with increased coronary sinus oxygen content, were observed for-ACh (+66 +/- 20%), BK (+74 +/- 24%), and RH (+24 +/- 5%) but not SNP (-2 +/- 10%). Production of NO metabolites (nitrate and nitrite), measured as their coronary arteriovenous differencexCBF, was significantly increased after 1 to 2 days of CAR, both at baseline (153 +/- 56%) and during BK infusion (220 +/- 76%) (P < .05). Holding CBF at pre-CAO levels during the initial CAR period did not attenuate the delayed enhanced endothelial vasodilation to ACh and BK. However, NO blockade with intracoronary NG-nitro-L-arginine blocked the enhanced coronary vasodilation to ACh and BK. Thus, in contrast to previous studies, these data indicate that brief ischemic episodes induce delayed enhanced coronary endothelial function, which is delayed in onset and prolonged in duration. This can be explained by an upregulation of coronary vascular NO production, potentially involved in the mechanism of the delayed window of preconditioning.
Possibility of Subelectron Noise With Room-Temperature Silicon Carbide Pixel Detectors

Pixel radiation detectors made on epitaxial silicon carbide are presented. Two small-format prototypes have been fabricated: a 4times4 matrix with pixels of 400times400 mum2 and 6times6 matrix with 200times200 mum2 pixels. Typical leakage currents between 3 fA and 10 fA for the larger pixel size, and between 0.1 fA and 4 fA for the small pixels have been measured at 27 degC, corresponding to current densities between 0.25 and 10 pA/cm2. In terms of equivalent noise charge, the contribution of most of the pixels is lower than 1 electron root mean square (rms) up to peaking times of tens of mus of pulse shaping. These pixel detectors are ready for applications in ultimate-resolution X-ray spectroscopic imaging at room temperature when a suitable ultra low noise front-end electronics, presently not available, will be developed. An analysis of the experimental data on these detectors coupled to front-end transistors of commercial CMOS technologies is presented, indicating that a noise level of around 4-electrons rms can be achieved at room temperature, limited by the front-end 1/f noise. The conditions to fully exploit the SiC pixel capabilities are quantitatively analysed by considering CMOS technologies for front-end and preamplifier design and the continuous progress in the SiC growing processes
Lactobacillus brevis OPK-3 isolated from kimchi inhibits adipogenesis and exerts anti-inflammation in 3T3-L1 adipocyte.

BACKGROUND Kimchi is a traditional fermented food in Korea that contains various unique microorganisms. Diverse bacteria are involved in the process of Kimchi fermentation and the healthful advantages; one of the major species is Lactobacillus. We investigated whether lactic acid bacteria isolated from Kimchi (KLAB) are capable of reducing intracellular lipid accumulation by downregulating the expression of adipogenesis and lipogenesis promoting genes in differentiating 3T3-L1 cells. RESULTS KLAB (Lactobacillus brevis OPK-3) mediated dose-dependent inhibition of adipocyte differentiation, intracellular triglyceride accumulation and glycerol-3-phosphate dehydrogenase (GPDH) activity. The expression of transcription factors such as peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α involved in adipogenesis was markedly decreased by the KLAB treatment. Terminal adipogenic marker, e.g. adipocyte fatty acid binding protein (aP2), lipoprotein lipase, liver X receptor α, leptin and GPDH were significantly downregulated by KLAB treatment compared to untreated control. Moreover, cytokine genes, such as tumor necrosis factor-α and interleukin-6 mRNA expressions level were also decreased, whereas adiponectin mRNA level was upregulated by KLAB. CONCLUSION These results suggest that the KLAB inhibits lipid accumulation in the differentiating adipocyte through downregulating the expression of adipogenic transcription factors and other specific genes involved in lipid metabolism.
Regulation of the ITGA2 gene by epigenetic mechanisms in prostate cancer

Integrin alpha2 beta1 (α2β1) plays an integral role in tumour cell invasion, metastasis and angiogenesis, and altered expression of the receptor has been linked to tumour prognosis in several solid tumours. However, the relationship is complex, with both increased and decreased expression associated with different stages of tumour metastases in several tumour types. The ITGA2 gene, which codes for the α2 subunit, was examined to investigate whether a large CpG island associated with its promoter region is involved in the differential expression of ITGA2 observed in prostate cancer.
Nuclear and cytoplasmic expression of ErbB-4 in prostate cancer.

PURPOSE To evaluate cytoplasmic and nuclear ErbB-4 expression in prostate cancer specimens and its association with outcome. BASIC PROCEDURES Specimens of 50 prostate cancer patients were investigated for ErbB-4 overexpression using Immunohistochemistry staining. Cytoplasmic and nuclear staining was graded as 0-3 according to its intensity. The prognostic parameters were tumor stage, PSA level, Gleason score, probability of positive lymph nodes (Partin's tables and Roach equation), and 5-year disease free survival (Kattan nomogram). MAIN FINDINGS Overexpression of ErbB-4 (> or = 1) was detected in 30 (60%) patients and overexpression using cytoplasmic and nuclear staining was > or = 2 in 19 (38%) and 17 (34%) patients, respectively. In only one third of the specimens was there any similarity between the 2 types of staining. Advanced tumor stage, high pretreatment PSA levels and high Gleason scores were evenly distributed among the patients with low (< or = 1) and intermediate/high (> or = 2) ErbB-4 expression. The probability of lymph node involvement and 5-year disease free survival were similar in both types of staining. PRINCIPAL CONCLUSIONS ErbB-4 was overexpressed (cytoplasmic and nuclear staining) in approximately one third of prostate cancer patients. The rate of similarity between the 2 staining types was only 33%: overexpression was evenly distributed among intermediate/high and low risk prostate cancer patients with both staining methods.
Aligning technology and institutional readiness: the adoption of innovation

ABSTRACT This paper explores and develops the concept of ‘readiness’ as it relates to the adoption of innovation. In particular, the paper discusses readiness in regard to the notion of ‘technology readiness’ levels, widely used today by both producers and users to monitor and manage emergent innovation. The paper argues that, while useful, this notion needs to be informed by and subsumed within a broader concept of ‘institutional readiness’. The latter is especially important in conceptualising how new technologies are actually adopted in organisational settings. The paper develops a model of institutional readiness that recognises the saliency of technology readiness but which embeds it within a broader socio-technical framework. This is illustrated with reference to the emerging field of regenerative medicine.
The Police, the Immigration Office and Illegal Immigrants: Indonesian’s Cases

As a country that has strategic position, Indonesia faces illegal trafficking of people and goods. If the illegal traffic is not taken seriously, not only the country will lose revenue from customs duties, but also not integrated efforts against illegal traffic of goods and people. This paper will discuss the role of POLRI in assisting the Immigration Office in combating human trafficking and smuggling. This paper will also discuss the problem of illegal immigrants and human trafficking is complicated due to unscrupulous government and security apparatus. This paper also seeks actors involved and their role in paving the way for illegal immigrants. Finally, this paper argue the duty of POLRI in assisting the Immigration Office will run effectively if it involves a number of government agencies relating to the management and supervision of foreign territories, including the Ministry of Home Affair and the Armed Forces (TNI).
Intravaginal inoculation of rhesus macaques with cell-free simian immunodeficiency virus results in persistent or transient viremia

The simian immunodeficiency virus (SIV)-rhesus macaque model of heterosexual human immunodeficiency virus transmission consists of atraumatic application of cell-free SIVmac onto the intact vaginal mucosa of mature female rhesus macaques. This procedure results in systemic infection, and eventually infected animals develop the clinical signs and pathologic changes of simian AIDS. To achieve 100% transmission with the virus stocks used to date, multiple intravaginal inoculations are required. The current titration study utilized two stocks of SIVmac and demonstrated that a single intravaginal dose of cell-free SIV can reliably produce infection in rhesus macaques. This study also demonstrated that some animals intravaginally inoculated with cell-free SIVmac develop transient viremia characterized by a limited ability to isolate virus from peripheral blood mononuclear cells and lymph node mononuclear cells and no seroconversion to SIV antigen. SIV could be isolated from the peripheral lymph nodes of transiently viremic animals only during periods of viremia and not at times when SIV was not detected in circulating mononuclear cells. Thus, peripheral lymphoid tissues were not reservoirs of infection in the transiently viremic animals. Taken together, these results suggest either that the SIV infection was cleared in the transiently viremic animals or that SIV infection is limited to a compartment of the genital mucosal immune system that cannot be assessed by monitoring SIV infection in peripheral blood mononuclear cells and peripheral lymphoid tissue.
Antifungal Prophylaxis in Liver Transplantation: A Systematic Review and Network Meta‐Analysis

Invasive fungal infections (IFIs) cause significant morbidity and mortality in liver transplant recipients, but the need and best agent for prophylaxis is uncertain. A comprehensive literature search was performed to identify randomized controlled trials comparing regimens for antifungal prophylaxis in liver transplant recipients. Direct comparisons were made between treatments using random‐effects meta‐analysis and a Bayesian network meta‐analysis was performed for the primary end point of proven IFI. Fourteen studies met inclusion criteria, reporting comparisons of fluconazole, liposomal amphotericin B (L‐AmB), itraconazole, micafungin and placebo. Overall, antifungal prophylaxis reduced the rate of proven IFI (odds ratio [OR] 0.37, confidence interval [CI] 0.19–0.72, p = 0.003), suspected or proven IFI (OR 0.40, CI 0.25–0.66, p = 0.0003) and mortality due to IFI (OR 0.32, CI 0.10–0.83, p = 0.02) when compared to placebo. All‐cause mortality was not significantly affected. There was no difference in risk of adverse events requiring cessation of prophylaxis (OR 1.11, 95% CI 0.48–2.55, p = 0.81). In the network meta‐analysis an equivalent reduction in the rate of IFI was seen with fluconazole (OR 0.21, CI 0.06–0.57) and L‐AmB (OR 0.21, CI 0.05–0.71) compared with placebo. Routine prophylaxis with fluconazole or L‐AmB reduces the incidence of IFI following liver transplantation, and the available evidence suggests that the two are equivalent in efficacy.
Tumor necrosis factor causes increased pulmonary permeability and edema. Comparison to septic acute lung injury.

Tumor necrosis factor alpha (TNF), a monokine produced by mononuclear cells in response to bacterial endotoxin (LPS), creates a syndrome similar to septic shock in animal models. To study whether TNF could induce acute lung injury similar to that seen in gram-negative sepsis, we injected recombinant human TNF (rHuTNF alpha) into guinea pigs and monitored arterial blood gases, leukocyte counts, and left atrial (Pla), pulmonary artery (Ppa), and mean arterial pressures (MAP) serially for 8 h. Pulmonary histopathology was assessed microscopically, and cell counts and 125I-labeled albumin (125I-albumin) in bronchoalveolar lavage (BAL) fluid and lung wet/dry weight ratios were determined. Five groups of animals were studied; the 2 TNF groups received high (1.4 X 10(6) U/kg) or low (1.0 X 10(6) U/kg) doses of rHuTNF alpha, the sepsis group received 2 X 10(9) Escherichia coli/kg intravenously, and the control group received saline. An LPS control group receiving 40 ng/kg E. coli LPS was also included because the rHuTNF alpha contained a small amount of LPS as a contaminant. Pulmonary permeability was assessed by studying the Pla and the BAL fluid/plasma 125I-albumin ratio (permeability index). The permeability index was significantly increased in the high-dose TNF (0.0408 +/- 0.0041, p less than 0.05) and sepsis groups (0.0466 +/- 0.0068, p less than 0.01) relative to controls (0.0215 +/- 0.0028). The wet/dry lung weight ratios were also significantly increased in the high-dose TNF (6.07 +/- 0.29, p less than 0.05) and sepsis groups (6.22 +/- 0.30, p less than 0.05) relative to the control group (5.18 +/- 0.20). (ABSTRACT TRUNCATED AT 250 WORDS)
Growth and Debt in Angola at Provincial Level

This paper analyses GDP growth and public debt at the provincial level in Angola from 2004-2015, using a spatial model. First a SAC-Spatial autocorrelation model panel is estimated. Later, a robust test is adopted estimating the Hans-Philips linear spatial dynamic model. Finally, a spatial 3sls model is estimated taking into account the possibility endogeneity of regional spatial autocorrelation. The three models give similar results revealing that in Angola public expenditure increase GDP growth but debt decrease it.
Clinical relevance of residual disease monitoring by polymerase chain reaction in patients with ALL‐1/AF‐4 positive‐acute lymphoblastic leukaemia

In this study we used reverse transcriptase‐polymerase chain reaction (RT‐PCR) for the longitudinal monitoring of minimal residual disease in 12 patients with ALL‐1/AF‐4 positive ALL. Of these, seven also showed at presentation a typical t(4;11) cytogenetic translocation. Seven patients were infants <18 months of age and five were adults.
A Randomized, Double-Blind, Placebo-Controlled, Fixed-Dose, Multicenter Study of Pregabalin in Patients With Generalized Anxiety Disorder

Pregabalin is a novel compound under development for the treatment of several types of anxiety disorders. To obtain an initial evaluation of the efficacy and safety of pregabalin in the treatment of generalized anxiety disorder (GAD), we conducted a double-blind, fixed-dose, parallel-group, placebo and active-controlled multicenter 4-week study that compared 271 patients randomized to receive pregabalin 50 mg tid (N = 70), pregabalin 200 mg tid (N = 66), placebo (N = 67), or lorazepam 2 mg tid (N = 68), followed by a 1-week double-blind taper. The primary efficacy parameter was change from baseline to endpoint (last observation carried forward) in the Hamilton Anxiety Scale (HAM-A) total score; adjusted mean change scores on the HAM-A were significantly improved for pregabalin 200 mg tid (difference of 3.90 between drug and placebo; p = 0.0013 [ANCOVA], df = 252) and for lorazepam (difference of 2.35; p = 0.0483 [ANCOVA], df = 252), with the significant difference between the pregabalin 200 mg tid and placebo groups seen at week 1 of treatment (p = 0.0001 [ANCOVA], df = 238). Safety analysis, which included assessment of spontaneously reported adverse events, laboratory monitoring, and withdrawal symptoms, showed pregabalin to be generally well-tolerated. The most common adverse events seen with pregabalin 200 mg tid were somnolence and dizziness. They were usually mild or moderate in intensity and were often transient. Pregabalin-treated patients had a higher completion rate than lorazepam-treated patients. This study supports the hypothesis that pregabalin is effective and safe in short-term therapy for GAD. More studies are needed to determine the best dosing regimen to optimize efficacy and tolerability.
KPZ formula derived from Liouville heat kernel

In this paper, we establish the Knizhnik–Polyakov–Zamolodchikov (KPZ) formula of Liouville quantum gravity (LQG), using the heat kernel of Liouville Brownian motion. This derivation of the KPZ formula was first suggested by F. David and M. Bauer in order to get a geometrically more intrinsic way of measuring the dimension of sets in LQG. We also provide a careful study of the (no)‐doubling behaviour of the Liouville measures in the appendix, which is of independent interest.
The political economy of high skills: higher education inknowledge societies

A successful transition into the knowledge economy is said to depend upon higher level skills, creating unprecedented pressure on university systems – as they expand across countries – to provide knowledge-based labour markets with the skills needed. But what are the political economy dynamics underlying national patterns of high skill formation? This thesis argues that existing theoretical approaches are not well-suited to answer the question: ideational and structuralist frameworks downplay persistent national differences, while institutionalist accounts assume that national differences rest upon the very lack of higher education expansion in some countries, downplaying the crossnational trend of higher education expansion. The thesis proposes a framework that accounts for distinct national trajectories of high skill formation within the convergent trend of higher education expansion. In particular, two crucial variables are identified to theorise the relationship between higher education systems and knowledge-based labour markets: (i) the predominant type of knowledge economy in a given country; and (ii) the degree of inter-university competition across different higher education systems. It is argued that the former explains what type of higher level skills will be sought by employers and cultivated by governments, while the latter helps understanding of why some higher education systems are more open at the outset to satisfy labour market demands compared to others, determining whether institutional change in a given higher education system is likely to be encompassing or marginal. Cross-national descriptive statistics and systematic process analysis across a set of diverse country case studies (Britain, Germany and South Korea) are used to test the theory. By highlighting the agency of universities, governments and businesses and by linking higher education policy with knowledge-based growth strategies, this thesis provides a theoretical and empirical contribution on processes of institutional change in higher education and on broader trajectories of institutional change across advanced capitalist countries.
Corporate Social Responsibility and Business Value of Multinational Companies: Lessons from a Sub-Saharan African Environment

ABSTRACT The issue of corporate social responsibility (CSR) as an approach for businesses to independently take actions that lead to better levels of societal development as well as higher value creation for the business, particularly in developing countries has gained much advocacy. Thus, the purpose of this study is to examine the relationship between CSR and business value of multinational companies (MNCs) in sub-Saharan Africa. The study adopted quantitative research methodology and using multiple regression analysis, findings show that CSR can positively and significantly predict business values in the multinational subsidiaries. These values include direct (economic value) and indirect (human capital value and reputational business value). This paper therefore contributes to a novel CSR index from the perspective of business value and sustainability of MNCs in the Sub-Saharan Africa (SSA) environment. Thus, the paper recommends MNCs operating in Africa to enhance their social investment through their CSR strategy with the aim that CSR must not be regarded as a cost center, but an investment instrument that can accrue various dividends.