Miracle-dz/UniFAR-FTUs huggingface dataset:
StaRProtein, A Web Server for Prediction of the Stability of Repeat Proteins

Repeat proteins have become increasingly important due to their capability to bind to almost any proteins and the potential as alternative therapy to monoclonal antibodies. In the past decade repeat proteins have been designed to mediate specific protein-protein interactions. The tetratricopeptide and ankyrin repeat proteins are two classes of helical repeat proteins that form different binding pockets to accommodate various partners. It is important to understand the factors that define folding and stability of repeat proteins in order to prioritize the most stable designed repeat proteins to further explore their potential binding affinities. Here we developed distance-dependant statistical potentials using two classes of alpha-helical repeat proteins, tetratricopeptide and ankyrin repeat proteins respectively, and evaluated their efficiency in predicting the stability of repeat proteins. We demonstrated that the repeat-specific statistical potentials based on these two classes of repeat proteins showed paramount accuracy compared with non-specific statistical potentials in: 1) discriminate correct vs. incorrect models 2) rank the stability of designed repeat proteins. In particular, the statistical scores correlate closely with the equilibrium unfolding free energies of repeat proteins and therefore would serve as a novel tool in quickly prioritizing the designed repeat proteins with high stability. StaRProtein web server was developed for predicting the stability of repeat proteins.
Demographic Characteristics of Multiple Births in Hungary

In Hungary the frequency of multiple births has been decreasing. In 1920 the rate of multiple births was 23-5‰; by 1968 this rate decreased to 18.2‰, i.e., only 77% of the 1920 data. The male preponderance in twin births is lower than in single births, and in triplets actually a female excess can be observed. Furthermore, it can be observed that there are more boys than girls among the first-born of multiple births. The average birth weight of liveborn and stillborn twins, and of liveborn triplets is 2101 g, 1458 g, and 1723 g respectively. The ratio of MZ to DZ twins is about 30:70 in 1968. The frequency of DZ twin births increases with maternal age (the peak is now over 40 years) and birth order. According to the 1959, i960, and 1968 figures, both DZ and MZ twin births were significantly more frequent in mothers aged 16 or less than in mothers aged 17-19 years. This demographic survey belonged to preparatory works connected with the setting up of the Budapest Twin Register which came into force on January 1st, 1970.
Virus meningo-encephalitis in Austria. II. Clinical features, pathology, and diagnosis.

This paper reports on the clinical and pathological features as well as the diagnosis of 304 cases of virus meningo-encephalitis hospitalized at the Neurological Clinic, Graz, during the 1953 epidemic in Styria, Austria. Of these cases, 175 were of an aparalytic meningeal form, 50 preparalytic, 27 spinal paralytic, 20 bublo-spinal or ascending paralytic, 7 bulbar paralytic, 3 radiculitic or transmyelitic, and 22 encephalitic. The case-fatality rate was 4.6%. The clinical course of the disease-usually diphasic-and the changes in the spinal fluid showed a close similarity to those seen in poliomyelitis, but the paralysis preferentially involved the shoulder-girdle and upper arm.The post-mortem examination showed a picture of acute encephalitis, with changes in the grey matter particularly. Histological findings revealed extensive involvement of the anterior horns of the spinal cord, the medulla oblongata, and the pons, and especially of the Purkinje's cells of the cerebellum.In making a diagnosis, the possibility of louping-ill, Russian spring-summer encephalitis, Czech tick encephalitis, and, particularly, poliomyelitis must be taken into account. The author points out that it is impossible to distinguish the virus meningo-encephalitis of this epidemic from poliomyelitis on the basis of clinical and post-mortem findings alone; a differential diagnosis must rest on serological and immunological grounds.Finally, the symptomatic and supporting treatment given is described.
Acute and long-term clinical significance of myocardial contusion following blunt thoracic trauma: results of a prospective study.

BACKGROUND The clinical significance of myocardial contusion (MC) following blunt thoracic trauma is still unknown. Accordingly, in this prospective study in a regional trauma center we investigated the acute and long-term clinical significance of MC. METHODS One-hundred eighteen patients with manifest or suspected blunt thoracic trauma were evaluated for cardiac injury. Initial assessment was based on results of electrocardiographic, echocardiographic, and serial enzyme measurements. A follow-up (FU) assessment conducted at 3 and 12 months, respectively, was performed using electrocardiography, echocardiography, and a bicycle ergometry exercise test. RESULTS Of the 118 patients in the total sample, 67 (56.8%) required admission to the surgical intensive care unit (SICU) because of the severity of their noncardiac injuries. Fourteen patients of the total sample (11.8%) were diagnosed with MC and 13 of these (92.9%) belonged to the intensive care admissions, thus representing an incidence of 19.4% in this patient group. During the hospitalization period none of the patients with MC experienced acute cardiac complications. Eighty-six patients (72.0%) were assessed at FU. No new pathologies were found except in one patient. Exercise testing revealed no ECG abnormalities and none of the patients experienced limitations on the bicycle ergometer due to a cardiac cause. CONCLUSIONS The incidence of 19.7% of MC in our patients with blunt chest injury requiring intensive care treatment indicates that this condition is frequent in polytraumatized patients. Outcome and prognosis in patients with MC is favorable and, thus, routine cardiac work-up is not indicated. Specific diagnostic and therapeutic measures should be limited to cases where cardiac complications develop.
The Effects of a Periodized vs. Traditional Military Training Program on 2-Mile Run Performance During the Army Physical Fitness Test (APFT).

The purpose of this study was to compare the effects of an experimental versus traditional military run training on 2-mile run ability in Army Reserve Officer Training Corps (ROTC) cadets. Fifty college-aged cadets were randomly placed into two groups and trained for four weeks with either an experimental running program (EXP, n=22) comprised of RPE intensity-specific, energy system based intervals or with traditional military running program (TRA, n=28) utilizing a crossover study design. A 2-mile run assessment was performed just prior to the start, at the end of the first 4 weeks, and again after the second 4 weeks of training following crossover. The EXP program significantly decreased 2-mile run times (961.3s ± 155.8s to 943.4 ± 140.2s, P=0.012, baseline to post 1) while the TRA group experienced a significant increase in run times (901.0 ± 79.2s vs. 913.9 ± 82.9s) over the same training period. There was a moderate effect size (d = 0.61, P=0.07) for the experimental run program to "reverse" the adverse effects of the traditional program within the 4-week training period (post 1 to post 2) following treatment crossover. Thus, for short-term training of military personnel, RPE intensity specific running program comprised of aerobic and anaerobic system development can enhance 2-mile run performance superior of a traditional program while reducing training volume (60 min per session vs. 43.2 min per session, respectively). Future research should extend the training period to determine efficacy of this training approach for long term improvement of aerobic capacity and possible reduction of musculoskeletal injury.
Direct noise analysis of log-domain filters

A direct method for analysing noise in log-domain filters is proposed which exploits simplifying assumptions such as neglecting higher order noise terms, linearity in the current domain, the translinear principle and time-averaging. All noise sources are treated in situ, without the need to refer any to either the input or output. The results are compared to SpectreRF periodic noise simulations.
Exploration of disorder in protein structures by X‐ray restrained molecular dynamics

Conformational disorder in crystal structures of ribonuclease‐A and crambin is studied by including two independent structures in least‐squares optimizations against X‐ray data. The optimizations are carried out by X‐ray restrained molecular dynamics (simulated annealing refinement) and by conventional least‐squares optimization. Starting from two identical structures, the optimizations against X‐ray data lead to significant deviations between the two, with rms backbone displacements of 0.45 Å for refinement of ribonuclease at 1.53 Å resolution, and 0.31 Å for crambin at 0.945 Å. More than 15 independent X‐ray restrained molecular dynamics runs have been carried out for ribonuclease, and the displacements between the resulting structures are highly reproducible for most atoms. These include residues with two or more conformations with significant dihedral angle differences and alternative hydrogen bonding, as well as groups of residues that undergo displacements that are suggestive of rigid‐body librations. The crystallographic R‐values obtained are ≈ 13%, as compared to 15.3% for a comparable refinement with a single structure. Least‐squares optimization without an intervening restrained molecular dynamics stage is sufficient to reproduce most of the observed displacements. Similar results are obtained for crambin, where the higher resolution of the X‐ray data allows for refinement of unconstrained individual anisotropic temperature factors. These are shown to be correlated with the displacements in the two‐structure refinements.
Admission of critically ill patients with cancer to the ICU: many uncertainties remain

With the beginning of the 21st century, development in the early detection and management of patients with cancer has led to a significant increase in the survival rates of these patients.1 As a result, an increased number of patients with cancer are requiring admission to the intensive care unit (ICU) either for cancer-related complications or for treatment-associated side effects. Until recently, admission of patients with cancer to the ICU has been discouraged2 because of the risk of unfavourable outcome.3 ,4 According to the current literature, however, the number of patients with cancer who may benefit from ICU support has increased,5–9 while improved survival rates have been reported in various subgroups of patients previously supposed to be of adverse outcome, such as patients after haematopoietic stem cell transplantation, patients with acute respiratory failure, acute renal failure, even patients who received urgent chemotherapy while in the ICU.10 ,11 These new data prompt the need for reappraisal of the policies regarding ICU admission in critically ill patients with cancer. The decision as to whether or not to admit a critically ill patient with cancer to the ICU is quite often challenging. Their medical problems along with their baseline disease underscore a need for an individual approach to this patient population. The decision to apply life-sustaining technology in these patients involves weighing the potential benefit against a futile care. Identification of factors associated with outcomes would help physicians, patients and families in determining the goals of treatment. Risk factors for adverse outcome have been defined repeatedly. Reasons of admission, type of malignancy, number of organ failures on ICU admission, need for …
Drug-Related Problem Classification Systems

OBJECTIVE To provide an overview of and critically appraise classifications of drug-related problems (DRPs) for use during the pharmaceutical care process and research in pharmacy. DATA SOURCES A literature search was conducted using MEDLINE and Yahoo (January 2003) and manually. The search terms included DRP, drug-related problem, drug-therapy problem, and medicine-related problem. STUDY SELECTION AND DATA EXTRACTION English- and German-language articles on pharmaceutical care and DRPs were reviewed. DATA SYNTHESIS Most classifications of DRPs were identified through searching publications on pharmaceutical care and DRPs. Fourteen classifications with different focuses were found. Some classifications were hierarchical, categorized into main groups and subgroups. Various terminologies and definitions for DRPs were revealed, as well as guidelines for an optimal DRP classification. Classifications were assessed according to a clear definition, published validation method, and results reflecting process and outcomes, usability in pharmaceutical care practice, and a hierarchical structure with main groups and subgroups. CONCLUSIONS Finding DRP classifications by computerized search of the biomedical literature with the help of PubMed proved to be difficult. No classification could be found that met all of our criteria for an optimal system. Few classifications have been validated. Three have been tested as to their usability in practice and internal consistency. The Pharmaceutical Care Network Europe system Version 4 comes closest to the defined requirements.
Substitutions in the Glycoprotein (GP) of the Candid#1 Vaccine Strain of Junin Virus Increase Dependence on Human Transferrin Receptor 1 for Entry and Destabilize the Metastable Conformation of GP

ABSTRACT Candid#1 (Cd1) is an attenuated vaccine strain of Junin virus, the causative agent of Argentine hemorrhagic fever. Although several substitutions are present in Cd1, their importance for attenuation has not been established. We functionally characterized the substitutions present in the Cd1 glycoprotein (GP) and identified F427I in the transmembrane domain of the GP2 subunit as reducing infectivity in a reconstituted viral system. We further showed that this phenotype derives from the destabilization of the GP metastable conformation. Lastly, we identified an increased dependence of Cd1 GP on human transferrin receptor type 1 (hTfR-1) for entry, which may affect the tropism of the attenuated strain in vivo.
Athletes' precompetitive sleep behaviour and its relationship with subsequent precompetitive mood and performance

Abstract This investigation examined precompetitive sleep behaviour of 103 athletes and how it relates to precompetitive mood and subsequent performance. Results revealed that on the night before competition athletes slept well under the recommended target of eight hours of sleep for healthy adults, with almost 70% of athletes experiencing poorer sleep than usual. It was found that anxiety, noise, the need to use the bathroom and early event times were amongst the most commonly reported causes of disrupted sleep in athletes on the night prior to competition. The negative moods of fatigue and tension were both significantly negatively correlated with precompetitive relative sleep quality (r = −0.28, P = 0.004, r = −0.21, P = 0.030, respectively) and total sleep time (r = −0.23, P = 0.023, r = −0.20, P = 0.044, respectively). Additionally, tension was positively correlated with number of awakenings (r = −0.20, P = 0.045). Vigour was seen to be significantly positively associated with relative sleep quality (r = 0.24, P=0.013). The relationships between relative sleep quality and fatigue, tension and vigour accounted for approximately 4 − 5% of the variance in mood scores. Disrupted sleep did not demonstrate any significant relationship with relative sporting performance. Conclusions from the present investigation are that athletes may be at particular risk of disrupted sleep on the night prior to competition, and this disruption can negatively relate to an athlete's precompetitive mood states.
A low power low noise VCO and a high gain LNA for WSN in 130nm CMOS RF technology

This paper mainly focuses on the performance analysis of two core building blocks of a WSN transceiver system. A typical WSN transceiver consists of many blocks of which LNA and VCO are of major importance. These two blocks are design and simulated to operate at 2.4GHz ISM band especially for WSN using 130nm CMOS RF technology. Both the LNA and VCO are designed to achieve low power and high efficiency along with less number of components as to take less area as well. The cascoded common source topology is used with inductive source degeneration to design the LNA. The minimum noise figure (NFmin) of the proposed LNA is nearly 0.472 dB and gain i.e S21 is 15.390 dB, which is excellent for WSN application. The designed LNA is unconditionally stable over the frequency range of 1 GHz to 5 GHz. The VCO is designed with current reuse -Gm cross coupled topology to achieve phase noise of as low as -130dB at 1MHz offset frequency with an extremely low power consumption of about 0.023mw. The tuning voltage (Vtune) is set accordingly for 2.4 GHz WSN application. The proposed VCO shows excellent optimization performance for low power and low phase noise along with a FOM of about -214.69 dBc/Hz which proves to be very good for a compact WSN transceiver system.
Multi-Section Bow-Tie Steps for Full-Band Waveguide Polarization Rotation

A new compact multi-section structure for broadband waveguide polarization rotation is presented. The twist component is based on cascading multiple steps with bow-tie shape geometry, which are progressively rotated between the input and output rectangular waveguides. The twists show an excellent performance in the full operating frequency band, which is controlled with the number of sections and their shapes and can be designed for any rotation angle. Moreover, the bow-tie sections are very short and compact and can be easily manufactured, being well-suited for satellite applications. The proposed configuration is shown with design tables and curves for different rotation angles. Two 90° twists in WR75 waveguide have been tested, showing measured return losses of 30 dB (one section) and 40 dB (two sections) from 10 to 15 GHz with a total length of 5.6 mm and 8.6 mm, respectively.
BLOOD COMPONENTS: A novel approach to pathogen reduction in platelet concentrates using short‐wave ultraviolet light

BACKGROUND: Transfusion of platelet concentrates (PCs) is the basic treatment for severe platelet disorders. PCs carry the risk of pathogen transmission, especially bacteria. Pathogen reduction (PR) by addition of photochemical reagents and irradiation with visible or ultraviolet (UV) light can significantly reduce this risk. We present a novel approach for PR in PCs employing UVC light alone.
c‐FLIPL is a dual function regulator for caspase‐8 activation and CD95‐mediated apoptosis

Activation of the caspase cascade is a pivotal step in apoptosis and can occur via death adaptor‐mediated homo‐oligomerization of initiator procaspases. Here we show that c‐FLIPL, a protease‐deficient caspase homolog widely regarded as an apoptosis inhibitor, is enriched in the CD95 death‐inducing signaling complex (DISC) and potently promotes procaspase‐8 activation through hetero‐dimerization. c‐FLIPL exerts its effect through its protease‐like domain, which associates efficiently with the procaspase‐8 protease domain and induces the enzymatic activity of the zymogen. Ectopic expression of c‐FLIPL at physiologically relevant levels enhances procaspase‐8 processing in the CD95 DISC and promotes apoptosis, while a decrease of c‐FLIPL expression results in inhibition of apoptosis. c‐FLIPL acts as an apoptosis inhibitor only at high ectopic expression levels. Thus, c‐FLIPL defines a novel type of caspase regulator, distinct from the death adaptors, that can either promote or inhibit apoptosis.
Reverse Pricing : The Impact of Sophisticated Consumers , Seller Commitment , and Competition *

With a reverse-pricing mechanism (sometimes referred to as a Name-Your-Own Price or NYOP format), a prospective buyer submits a bid for a seller’s product, and a sale occurs only if the bid exceeds a hidden threshold price. This paper models the impact of three factors in such a market: 1) customer sophistication, i.e., whether buyers can correctly anticipate the seller’s procedure for setting the threshold price; 2) seller commitment, i.e., whether the seller commits to randomly selecting the threshold price from a distribution of possible prices; and 3) competition, i.e., whether the seller faces a rival. Some key findings are: in the absence of seller commitment, the seller profits more if customers are less sophisticated; seller commitment can improve profit, but only if customers are sophisticated; a monopolist seller benefits from allowing buyers to re-bid if their previous bid was rejected, but in the presence of both competition and seller commitment, the NYOP seller may benefit from restricting consumers to a single bid. Finally, whereas, the current literature offers little insight into why a firm would employ a NYOP business model rather than simply posting prices, this paper suggests that the NYOP mechanism may offer a way to mitigate price competition with a rival. This finding leads to important new implications for structuring the NYOP channel. For example, a seller may be able to reduce the degree of price rivalry by committing to randomly selecting its price threshold and/or by prohibiting re-bids.
Solute Transport Processes in Wetlands Application of Data Based Mechanistic and Transient Storage Models

The aim of this paper is the analysis of solute transport processes in Upper Narew River based on the results of tracer experiment. Data Based Mechanistic and transient storage models were applied to Rhodamine WT tracer observations. We focus on the analysis of uncertainty and the sensitivity of model predictions to varying physical parameters, such as dispersion and channel geometry. The study is based on a combined Global Sensitivity Analysis (GSA) and Generalized Likelihood Uncertainty Estimation (GLUE). The breakthrough curves for the chosen cross-sections are compared with those simulated with 95% confidence bounds. Apart from the predictions of the pollutant transport trajectories, two ecological indicators are also studied (time over the threshold concentration and maximum concentration). These indicators show an interesting multi-modal dependence on model parameters.