Miracle-dz/UniFAR-FTUs huggingface dataset:
Identification and characterization of a baculovirus structural protein, VP1054, required for nucleocapsid formation

The defect in a temperature-sensitive mutant of Autographa californica nuclear polyhedrosis virus, tsN1054, was mapped and characterized. At the nonpermissive temperature of 33 degrees C, this mutant fails to form plaques upon infection of Sf-21 cultured insect cells; infection is limited to a single cell, even though the infection proceeds through the very late phase. Marker rescue mapping and DNA sequencing identified the gene, ORF 54, which was altered by a single nucleotide substitution in tsN1054. Transcriptional analysis of the ORF 54 region identified multicistronic RNAs, from early to very late times of infection, that potentially encode the ORF 54 gene product. Polyclonal antiserum raised to a TrpE-VP1054 fusion protein recognized a 42-kDa late protein, VP1054, in infected-cell lysates. VP1054 was found to be a component of both budded virus and occlusion-derived virions. The level of VP1054 was dramatically reduced in tsN1054-infected Sf-21 cells propagated at 33 degrees C, and electron microscopic analysis of these cells showed that nucleocapsids failed to form in the nuclei of these infected cells. Instead, novel round, electron-dense bodies were found associated with the virogenic stroma in tsN1054-infected cells. Therefore, VP1054 is a virus structural protein required for nucleocapsid assembly.
An Assessment of the Antioxidant and Antimicrobial Activity of Six Species of Edible Irish Seaweeds

Six species of edible Irish seaweeds; Laminaria digitata, Laminaria saccharina, Himanthalia elongata, Palmaria palmata, Chondrus crispus and Enteromorpha spirulina were screened for potential bioactivity. Extraction of secondary metabolites was carried out using different solvents to determine antioxidant and antimicrobial properties of the dried extracts. The total phenolic contents of dried methanolic extracts were significantly different (p < 0.05). H. elongata exhibited highest phenolic content at 151.3 mg GAE/g of seaweed extract and also had the highest DPPH scavenging activity (p < 0.05) with a 50% inhibition (EC50) level at 0. 125μg/ml of extract. H. elongata also had the highest total tannin and total flavonoid contents (p < 0.05) of 38.34 mg CE/g and 42.5 mg QE/g, respectively. Antimicrobial activity was determined using a microtitre method which allowed detection of bacterial growth inhibition at low levels. All methanolic seaweed extracts inhibited the food spoilage and food pathogenic bacteria tested; Listeria monocytogenes, Salmonella abony, Enterococcus faecalis and Pseudomonas aeruginosa, except C. crispus extracts. It was found that dried methanolic extracts of red and green seaweeds had significantly lower antimicrobial activity than the brown species; H. elongata had the highest antimicrobial activity with up to 100% inhibition. In the studied work, the antimicrobial activity of red and green seaweed extracts significantly increased when ethanol and acetone were used as extraction solvents (p < 0.05). Inhibition of E. faecalis with C. crispus extracts increased from 39.28 to 100% when ethanol and acetone were applied as solvents.
Nanometer-scale elongation rate fluctuations in the Myriophyllum aquaticum (Parrot feather) stem were altered by radio-frequency electromagnetic radiation

The emission of radio-frequency electromagnetic radiation (EMR) by various wireless communication base stations has increased in recent years. While there is wide concern about the effects of EMR on humans and animals, the influence of EMR on plants is not well understood. In this study, we investigated the effect of EMR on the growth dynamics of Myriophyllum aquaticum (Parrot feather) by measuring the nanometric elongation rate fluctuation (NERF) using a statistical interferometry technique. Plants were exposed to 2 GHz EMR at a maximum of 1.42 Wm−2 for 1 h. After continuous exposure to EMR, M. aquaticum plants exhibited a statistically significant 51 ± 16% reduction in NERF standard deviation. Temperature observations revealed that EMR exposure did not cause dielectric heating of the plants. Therefore, the reduced NERF was due to a non-thermal effect caused by EMR exposure. The alteration in NERF continued for at least 2.5 h after EMR exposure and no significant recovery was found in post-EMR NERF during the experimental period.
Renal Blood Flow during Acute Renal Failure in Man

Background:In the critically ill, ischemia secondary to decreased renal blood flow (RBF) is believed to be central to the pathogenesis of acute renal failure (ARF); however, the scientific basis for this conclusion has not been systematically evaluated. Methods: Systematic interrogation of the Pubmed database, and screening bibliographies of retrieved reports, for studies of human ARF where RBF was measured. Results: Thirty-two articles published between 1944 and 2008 describing RBF in 373 patients with ARF were identified. Overall, mean RBF during ARF was 387 ml/min. It was 329 ml/min when estimated by clearance-based techniques (15 studies) and 471 ml/min when measured with nonclearance-based techniques (17 studies). Only 46 patients had measurements in the intensive care unit where mean RBF was 306 ml/min. Normal RBF was reported in 14 publications, mean 1,192 ml/min. Conclusions: Limited information is available on RBF during ARF in the critically ill. Measurements in contemporary patients are required to further our understanding of this condition.
RecMoodle - An Educational Recommender System

This paper proposes a recommender system for the Moodle platform that uses Apache Mahout as a recommendation engine. In this system, students receive personalized and adaptive recommendations based on forum discussions and learning materials available in the environment, according to their interactions. The recommendation method developed is based on the Moodle's activity log. In this paper, we compare four different ways to process user's preferences and present recommendations. Three of them exist in the literature, and one is proposed by us. The test set uses data from a real case scenario, i.e., the activity log from a graduation course that is given in our institution. We have found that the proposed approach produced the best set of recommendations.
Applying Genetic Algorithms to Pronoun Resolution

Introduction Many pronoun resolution algorithms work by calculating the most salient candidate antecedent. However, many factors affect salience, for example being the syntactic subject or the most frequently mentioned item, and these factors must be combined into an aggregate salience score. One technique is to assign weights for each factor representing the amount by which that factor impacts the overall salience, and the candidate antecedent which accumulates the most weight is selected. Previous authors assigned weights heuristically (cf. Mitkov 1998). By using a genetic algorithm to select the weights, our program beats baseline techniques, and can be customized for each language domain.1
Mass customisation and IT influence on Brazilian health industries' competitiveness

This article analyses the competitiveness of Brazilian medical products and pharmaceutical industries based on the strategic implementation of Information Technology (IT). The model of competitiveness developed by Boynton et al. (1993) – Dynamic Stability and the assessment of IT presence in Porter and Millar's (1985) Value Chain – are the theoretical references of this study. A field research was carried out simultaneously at the Marketing and IT departments of 14 Brazilian plants. Its main goals were to assess how those plants are reacting to the change of the competition pattern, to identify the customisation usage as a competitive differential, and last but not least, to ensure an intensified flow of computer resources. A comparative analysis of data obtained in different field research done in the USA and Brazil also illustrates the viewpoints.
Visual Event Mining from the Twitter Stream

Twitter is a unique microblog, which is different from conventional social media in terms of its quickness and on-the-spot-ness. Many Twitter's users send messages, which is commonly called "tweets", to Twitter on the spot with mobile phones or smart phones, and some of them send photos and geotags as well as tweets. Most of the photos are sent to Twitter soon after taken. In case of photos related to some events, most of them are taken during the events. We think that Twitter event photo mining is more useful to under- stand what happens currently over the world than only text-based Twitter event mining. In this paper, we propose a system to mine events visually from the Twitter stream. To do that, we use not only tweets having both geotags and photos but also tweets having geotags or photos for textual analysis or visual analysis. Although there exist many works related to Twitter mining using only text analysis such as typhoon and earthquake detection by Sakaki et al. [1], only a limited number of works exist on Twitter mining using image analysis. Nakaji et al. [2] proposed a system to mine representative photos related to the given keyword or term from a large number of geo-tweet photos. They extracted representative photos related to events such as "typhoon" and "New Year's Day". They used only geotagged photo tweets the number of which are limited compared to all the photo tweets. Gao et al. [3] proposed a method to mine brand product photos fromWeibo which employs supervised image recognition, which is different from event detection. They integrated visual features and social factors (users, relations, and locations) as well as textual features for brand product photo mining. In this paper, we detect visual events using geotagged non-photo tweets and non-geotagged photo tweets as well as geotagged photo tweets. In the experiments, we show some examples of detected events and their photos such as "rainbow", "fireworks" and "festival".
An Investigation of Patients' Perceptions of Nursing Care: Case of Intensive Care

Introduction: Intensive care units are different from other hospital areas due to the nature of patients and illnesses, treatment methods, and physical and emotional environment. This difference can also create differences in nursing care and patients’ perceptions of this care. The present study aims to identify intensive care patients’ perceptions of nursing care. Method: Target population of this descriptive and cross sectional s tudy was all patients who were in the intensive care units of five hospitals ( 1 research hospital, 1 training hospital, 3 state hospitals) with different status in Adana/Turkey . The participants were 368 patients who met the research criteria. The data wer e collected through “Personal Information Form” and “ Patient Perception of the Quality of Nursing Care (PPQNC)” which identified patients’ perceptions of nursing care . Results: The participants were 368 patients, 148 (40.2%) female and 220 (59.8%) male , the average age of the participants was 56.9±15.7. T he median of the PPQNC total score was found 73 (70.56±6.80). There was a statistically significant relationship between PPQNC scores and the hospitals where the study was conducted (p=0.016). Mean rank values were higher in married patients w hen compared to single patients (p=0.031), in patients with income higher than expenses when compared to patients with income less than expenses (p=0.013) and with income equal to expenses (p=0.017), in illiterate patients when compared to the patients who graduated from primary school (p=0.023) and high school (p=0.040) , and in patients who graduated from university when compared to the patients who graduated from primary school (p=0.029 ). Conclusion: It was found that PPQNC scores were high and patient’s s atisfaction levels with the nursing care they receive d were good. Keyw ords: intensive care, nursing care , care perception
Bio-logiques du vieillissement

Le vieillissement n'est pas une maladie. Il atteint tout animal depassant Un certain âge et il concerne presque toutes les especes vivantes. Pourtant les tumeurs malignes et les maladies neurodegeneratives augmentent avec l'âge. On ne meurt pas de vieillesse. On meurt de poly-pathologies. Alors, ou est la poule, ou est l'œuf ? Ou est le vieillissement, ou est la maladie ? Le vieillissement, ce sont les atrophies musculaires et digestives. Mais est-ce l'atrophie digestive qui provoque la denutrition ou la denutrition qui la produit ? Il y a des cercles vicieux du vieillissement. Le cercle vicieux n'est pas un cycle. C'est un phenomene irreversible qui est propre a un systeme d'objets agissant sur lui-meme. Le vieillissement n'est pas une simple destruction. C'est une autodestruction. On se souvient que certains tissus peuvent regenerer leurs cellules. Mais ce mecanisme fonctionne de moins en moins bien. Les tissus fonctionnels sont peu a peu remplaces par des tissus conjonctifs. N'est-il pas surprenant alors de retrouver, au cœur de cette dynamique de destruction des cercles vicieux moleculaires ? Nous defendons ici une double hypothese. Scientifiquement, tout d'abord, nous souhaitons montrer que ces processus d'autodestruction ne sont ni stochastiques, ni genetiques. Ils sont epigenetiques. Philosophiquement ensuite, nous les considerons comme des processus emergents. Un systeme d'objets qui agit sur lui-meme n'est pas pourvu d'une âme ou d'un esprit frappeur. Il est simplement caracterise par le fait que son action vient completer sa description. C'est cela qui determine le sens qu'il faut donner selon nous, au concept d'emergence et qui n'est pas son sens usuel. L'autodestruction n'est donc pas la au commencement. Elle n'est pas programmee genetiquement. Elle resulte de la destruction de certaines molecules et ces effets que cette destruction entraine. Mais cette resultante est autre chose qu'une simple deduction. Et cette bio-logique du vieillissement est autre chose qu'une simple logique.
DEGENERATION OF THE PRIMARY AND SECONDARY SENSORY NEURONES AFTER TRIGEMINAL INJECTION

This paper comprises a clinical and pathological report of a case of tic douloureux, in which death occurred three and a half months after a successful injection of the right trigeminal nerve. Though this form of injection has been practised for nearly 40 years, there is still dispute about the exact site of action of the alcohol, the changes which it produces in the trigeminal pathway, and their duration.
Single-feed circularly polarized triangular microstrip antennas

Novel designs of single-feed circularly polarized microstrip antennas using slotted equilateral-triangular patches have been successfully demonstrated. Both cases of regular-size and compact circular polarization (CP) operations using the designs with, respectively, a narrow horizontal slot and a cross slot of unequal slot lengths embedded in the triangular patch are experimentally investigated. For the compact CP design, an antenna size reduction of about 17% as compared to the regular-size CP design is also obtained.
Comparison of balance ability of minimization, simple and stratified block randomization from true data of a clinical trial

Objective: Simple randomization, stratified block randomization and minimization are three most commonly used allocation methods in clinical trials. Our objective was to make a comparison of their balance ability from the true data of a clinical trial with a serials observational point from 50 to 1350 patients in 552 combined strata. Study design: We created random sequences by SAS plan program, and then allocated patients by MINI (Minimization), SB4 (Stratified Block randomization with block length of 4) and SR (Simple Randomization) when the sample size reach 50, 100, 300, 500, 800, 1000 and 1350 from a real clinical trial cohort and repeated 1000 times, standardized difference was used to evaluate the balance of baseline covariates. Results: When the number of subjects was small, minimization had the advantages against other two allocation methods. Stratified randomization achieved a slightly better balance result than simple randomization in given conditions. With the increasing number of patients, the difference between simple randomization and minimization or stratified block randomization decreased. Conclusions: We recommend minimization in small sample clinical trials when large number of combined strata exist, but prefer simple randomization when the sample size is large enough.
Discrimination between Streptococcus pneumoniae and Streptococcus mitis based on sorting of their MALDI mass spectra.

Accurate species-level identification of alpha-hemolytic (viridans) streptococci (VGS) is very important for understanding their pathogenicity and virulence. However, an extremely high level of similarity between VGS within the mitis group (S. pneumoniae, S. mitis, S. oralis and S. pseudopneumoniae) often results in misidentification of these organisms. Earlier, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been suggested as a tool for the rapid identification of S. pneumoniae. However, by using Biotyper 3.0 (Bruker) or Vitek MS (bioMérieux) databases, Streptococcus mitis/oralis species can be erroneously identified as S. pneumoniae. ClinProTools 2.1 software was used for the discrimination of MALDI-TOF mass spectra of 25 S. pneumoniae isolates, 34 S. mitis and three S. oralis. Phenotypical tests and multilocus gene typing schemes for the S. pneumoniae (http://spneumoniae.mlst.net/) and viridans streptococci (http://viridans.emlsa.net/) were used for the identification of isolates included in the study. The classifying model was generated based on different algorithms (Genetic Algorithm, Supervised Neural Network and QuickClassifier). In all cases, values of sensitivity and specificity were found to be equal or close to 100%, allowing discrimination of mass spectra of different species. Three peaks (6949, 9876 and 9975 m/z) were determined conferring the maximal statistical weight onto each model built. We find this approach to be promising for viridans streptococci discrimination.
Neuroimaging signatures of frailty: A population‐based study in community‐dwelling older adults (the Atahualpa Project)

Frailty is a geriatric state of physical vulnerability that might be associated with cognitive decline in the absence of a concurrent neurodegenerative disorder. This assumes that neuroimaging studies are normal, but such examinations have rarely been considered for a frailty work‐up. The present study identifies neuroimaging signatures in older adults interviewed with the Edmonton Frail Scale (EFS).
L-Exclusion autostabilisante revisitée

La M-exclusion a pour but de partager L ressources identiques. Elle est definie par trois proprietes : l'equite, la surete et une propriete d'efficacite appelee evitement de L-interblocage. Nous montrons que tout algorithme realisant ces trois proprietes a un temps d'attente en Omega(n-L) rondes dans un anneau de n processus. Donc, quand n est grand compare a L, le gain d'avoir L ressources identiques au lieu d'une seule devient negligeable. Pour contourner ce probleme, nous reformulons la definition de L-exclusion en remplacant la propriete d'evitement de L-interblocage par une contrainte sur le temps d'attente. Nous illustrons cette nouvelle version du probleme avec des algorithmes autostabilisants dont le temps d'attente est en O(n/L) rondes, la borne asymptotique optimale.
Baseline information in structural failure time estimators for the effect of observed treatment compliance

Structural accelerated failure time models allow expression of the effect of treatment actually received in placebo‐controlled randomized trials with non‐compliance. Without further assumptions, the structural parameter is typically estimated via a series of auxiliary logrank tests, searching for the structural parameter that back‐transforms treated survival times to latent treatment‐free survival times which are equally distributed between randomized arms. In this paper we investigate to what extent score tests involving baseline covariates provide more powerful auxiliary tests and lead to more precise estimates of the structural parameter without compromising the α‐level. We propose a set of estimating equations which combines score components for covariate effects based on the control arm only, with a log‐likelihood score for treatment effect based on both arms. Analytic results for exponential models as well as simulation studies for the semi‐parametric approach indicate that in many practical situations this incorporation of baseline covariates leads to more precise estimators of the structural effect. Relative efficiency is shown to depend on the selective nature of compliance. In a leukaemia trial we find the length of the 95 per cent confidence interval for the structural parameter is reduced to two‐thirds of the original length by incorporating baseline covariates in this way. Copyright © 2002 John Wiley & Sons, Ltd.
[Electrophysiological study on the antiarrhythmic mechanism of ampelopsin in rats].

OBJECTIVE To explore the antiarrhythmic mechanism of ampelopsin through electrophysiological study in rats. METHODS The in vivo experimental groups were as follows:control group, low-dose, middle-dose and high-dose group. Arrhythmia in rats was induced by aconitine injection, and then the antiarrhythmic effects of ampelopsin were studied. Cardiomyocytes were isolated from rats therafter. The whole-cell patch-clamp technique was used to record action potential duration (APD), sodium currents (INa), calcium current (ICa), transient outward potassium currents (Ito) and inward rectifier potassium currents (IK1) in cardiomyocytes. RESULTS In vivo experiments showed that the incidence of aconitine-induced experimental arrhythmias in low, middle and high-dose ampelopsin group was significantly lower than that in control group (n = 5 each group, all P < 0.05). In vitro whole-cell patch clamp experiments showed that action potential duration in low, middle and high-dose groups was significantly shorter than that in control group, and amplitude of action potential was also significantly lower in low, middle and high-dose ampelopsin groups than in control group (134.1 ± 6.9), (120.1 ± 7.4), (113.2 ± 9.0), and (101.8 ± 5.1) mV for control, low, middle and high-dose group (n = 9 each group, all P < 0.05). Further research revealed that sodium currents in cardiomyocytes were decreased by low, middle and high-dose ampelopsin from (-36.75 ± 3.60) to (-31.03 ± 2.61), (-26.63 ± 3.72), and (-17.55 ± 4.43) pA/pF (n = 9 each group, all P < 0.05), but the activation voltage for peak potential was not affected by ampelopsin. Moreover, the inward rectifier potassium current was also higher in high-dose ampelopsin group than in control group (P < 0.05). Calcium current and transient outward potassium current were similar among four groups. CONCLUSION Ampelopsin exerts anti-arrhythmic effects in this rat model, and the underlying electrophysiological mechanism is partly associated with the inhibition of INa and enhancement of IK1, and prolongation of APD.