Miracle-dz/UniFAR-FTUs huggingface dataset:
Novel splice variants in the 5'UTR of Gtf2i expressed in the rat brain: alternative 5'UTRs and differential expression in the neuronal dendrites

General transcription factor II‐I (Gtf2i) is a transcription factor and one of the genes implicated in Willams–Beuren syndrome, an autism spectrum disorder. In this study, we investigated splice variants of the Gtf2i gene in both the 5'untranslated region (5'UTR) and the coding region. To search for novel 5'UTRs of Gtf2i, we utilized the cap analysis gene expression database of the mouse. We identified seven novel Gtf2i transcripts with alternatively spliced 5'UTRs in the rat brain. We also identified four novel splice variants in the coding sequence of Gtf2i. Furthermore, we identified a selective usage of certain types of 5'UTR by coding variants. In situ hybridization demonstrated a differential pattern of expression of Gtf2i mRNAs with alternatively spliced 5'UTRs among neuronal cells, and the localization of one of the variants in neuronal dendrites in the rat brain. Immunohistochemistry also demonstrated a distribution of Gtf2i‐immunoreactivity in the dendrites. These results suggest multiple pathways of expression of Gtf2i gene in the brain. The expression patterns may be under the control of alternative promoters coupled to the alternative splicing in the coding region. Differential localization of mRNA to neuronal dendrites suggests spatiotemporal‐specific translation at the post‐synaptic sites that is involved in transfer of synaptic activity to expression of specific sets of genes in the nucleus. Gtf2i is a transcription factor and implicated in Willams–Beuren syndrome. We identified seven novel Gtf2i transcripts with alternatively spliced 5'UTRs in the rat brain. In situ hybridization demonstrated a differential expression of Gtf2i mRNAs with different 5'UTRs in somas and dendrites of neuronal cells. Differential localization of mRNA to neuronal dendrites suggests spatiotemporal‐specific translation at the postsynaptic sites. The scheme shows genomic structure showing the positions of the potential transcription start tags (rDEC695, rDEC3D7, rDEC1D3, rDEC104, rDEC072 and rDEBE25). Newly identified exons (1.1–1.6) are shown with the white boxes. The distances from rDEC695‐5'end are indicated in bp.
Fuzzy trajectory tracking control of an autonomous air vehicle

The development and the implementation of a new guidance law are addressed for a six dimensional trajectory tracking problem, three dimensions for position tracking and three dimensions for velocity tracking, of a micro air vehicle. To generate the desired trajectory a virtual leader is defined which is moved in space. In the guidance law, position and velocity feedbacks are used by fuzzy controllers to generate two acceleration commands. Then, a fuzzy coordinator is applied to coordinate the acceleration commands. Nonlinear six-degree-of-freedom equations of motion are used to model the vehicle dynamics. Also, a bank-to-turn acceleration autopilot for vehicle is considered to follow the acceleration commands. Simulations were carried out to verify the performance of the system. The results demonstrate the ability of the vehicle to track the desired trajectory.
The Matrix Proteins Hasp and Hig Exhibit Segregated Distribution within Synaptic Clefts and Play Distinct Roles in Synaptogenesis

The synaptic cleft is the space through which neurotransmitters convey neural information between two synaptic terminals. This space is presumably filled with extracellular matrix molecules involved in synaptic function or differentiation. However, little is known about the identities of the matrix components, and it remains unclear how these molecules organize the matrix in synaptic clefts. In this study, we identified Hasp, a Drosophila secretory protein containing CCP and WAP domains. Molecular genetic analysis revealed that Hasp diffuses extracellularly and is predominantly captured at synaptic clefts of cholinergic synapses. Furthermore, Hasp regulates levels of DLG and the nAChR subunits Dα6 and Dα7 at postsynaptic terminals. Hasp is required for trapping of another matrix protein, Hig, which is also secreted and diffused in the brain, at synaptic clefts of cholinergic synapses; however, Hig is dispensable for localization of Hasp at synaptic clefts. In addition, in the brains of triple mutants for the nAChR subunits Dα5, Dα6, and Dα7, the level of Hig, but not Hasp, was markedly reduced in synaptic regions, indicating that these nAChR subunits are required to anchor Hig to synaptic clefts. High-resolution microscopy revealed that Hasp and Hig exhibit segregated distribution within individual synaptic clefts, reflecting their differing roles in synaptogenesis. These data provide insight into how Hasp and Hig construct the synaptic cleft matrix and regulate the differentiation of cholinergic synapses, and also illuminate a previously unidentified architecture within synaptic clefts. SIGNIFICANCE STATEMENT The synapse has been extensively studied because it is essential for neurotransmission. By contrast, the space between the synaptic terminals, the synaptic cleft, is still an undeveloped research area despite its ubiquity in synapses. In fruit fly brains, we obtained evidence that the matrix protein Hasp and the previously identified Hig, both of which are secreted extracellularly, localize predominantly to synaptic clefts of cholinergic synapses, and modulate the levels of nAChR subunits on postsynaptic membranes. However, Hasp and Hig play differential roles in matrix formation and exhibit segregated distribution within synaptic clefts. These results reveal the molecular mechanisms of synaptic matrix construction and illuminate a molecular architecture within synaptic clefts previously unrevealed in any animal species.
Estudio del crecimiento y supervivencia de Celtis australis L. en repoblaciones de terrenos agrícolas de la región de Murcia

Las caracteristicas de los suelos agricolas a reforestar influyen en el comportamiento de las especies forestales utilizadas en la repoblacion. Teniendo en cuenta ademas que una de las mas importantes decisiones a tomar en la planificacion de las repoblaciones es la eleccion de especies, conviene recopilar el mayor numero de datos sobre el comportamiento de las especies autoctonas susceptibles de ser utilizadas en las forestaciones de los terrenos agricolas abandonados. Dentro de este contexto se desarrolla el presente trabajo, que evalua la respuesta del almez (Celtis australis L.), durante los primeros anos post-plantacion en la provincia de Murcia.
Investigate the Effects Of Initial Laser Phase , FWHM on Jitter And Soliton Order For Long Haul Communication

Soliton are narrow and high intensity pulses which can retain their shape by compensating the effects of SPM and GVD. Transmission of solitons at data rate of Tb/s effected by different factors. This paper analyzed jitter factor by varying FWHM (full width half maximum) and lases phase. Simulation results for soliton order N=1 and N=2 are also obtained.
Estudio de carbones como electrodos en baterías Litio-Azufre de alta densidad de energía

En la presente Tesis Doctoral se aborda la tematica de las Baterias Li-S, uno de los sistemas de almacenamiento de energia mas estudiados en los ultimos anos en campos de investigacion como la Quimica, la Ciencia de los Materiales, la Ingenieria y la Energia, como muestra la Fig.5. 1 Aunque su descubrimiento se remonta al siglo pasado (1962), no es hasta el ano 2000 cuando la investigacion en estas baterias comienza a experimentar un mayor desarrollo. Las baterias Li-S se presentan como una de las mejores alternativas para poder suplir la demanda energetica creciente de nuestra sociedad, siendo la tecnologia mas prometedora para vehiculos electricos e hibridos y.
EEG Markers of Dementia with Lewy Bodies: A Multicenter Cohort Study.

Quantitative EEG (QEEG) has demonstrated good discriminative capacity for dementia with Lewy bodies (DLB) diagnosis as compared to Alzheimer's disease (AD) with a predictive value of 100% in a single cohort study. EEG in DLB was characterized by a dominant frequency (DF) in pre-alpha (5.5-7.5 Hz), theta, or delta bands and DF variability (DFV) >1.2 Hz, frequency prevalence (FP) pre-alpha in >40% and FP alpha in <32% of the epochs. To validate the aforementioned QEEG findings in independent cohorts of clinically diagnosed DLB versus AD patients, we analyzed EEG traces of 79 DLB and 133 AD patients (MMSE >20) collected from four European Centers. EEG traces from 19 scalp derivations were acquired as at least 10 min continuous signals and epoched in off-setting as series of 2-second-long epochs, subsequently processed by Fast Fourier Transform (frequency resolution 0.5 Hz). DLB patients showed EEG specific abnormalities in posterior derivations characterized by DF <8 Hz FP pre-alpha >50%, FP alpha <25%. DFV was >0.5 Hz. AD patients displayed stable alpha DF, DFV <0.5 Hz, FP pre-alpha <30%, and FP alpha >55%. DLB and AD differed for DF (p < 10-6), DFV (p < 0.05), FP pre-alpha (p < 10-12) and FP alpha (p < 10-12). Discriminant analysis detected specific cut-offs for every EEG mathematical descriptor; DF = 8, DFV = 2.2 Hz, FP pre-alpha=33%, FP alpha = 41% for posterior derivations. If at least one of the cut-off values was met, the percentage of DLB and AD patients correctly classified was 90% and 64%, respectively. The findings in this multicenter study support the validity of QEEG analysis as a tool for diagnosis in DLB patients.
Circulation of Aichi virus genotype B strains in children with acute gastroenteritis in India

SUMMARY Acute gastroenteritis (AG) is considered as one of the major health problems affecting humans of all ages. A number of viruses have been recognized as important causes of this disease. Recently, Aichi virus has been shown to play an aetiological role in sporadic infections and outbreaks of AG. A study on surveillance of enteric viruses was conducted during 2004–2008 in three cities in Maharashtra state, western India. A total of 1240 stool specimens from children aged ⩽8 years hospitalized for AG were screened for the presence of Aichi virus by RT–PCR of the 3C–3D junction region followed by sequencing for the identification of genotype. Aichi virus was detected at a prevalence of 1·1% in the <5 years age group and characterized as genotype B. This is the first report on the circulation of Aichi virus genotype B in India.
Can We Use Imipenem and Meropenem Vitek 2 MICs for Detection of Suspected KPC and Other-Carbapenemase Producers among Species of Enterobacteriaceae?

ABSTRACT Imipenem and meropenem Vitek 2 MICs were evaluated for a panel of 104 Enterobacteriaceae for identification of carbapenemase producers. The sensitivity and specificity values for the new CLSI interpretative criteria (CLSI document M100-S20-U, 2010) were 98% and 83% for imipenem and 76% and 83% for meropenem, respectively. We propose an algorithm that is highly sensitive (98%) and specific (94%) for carbapenemase screening based on the combined use of imipenem and meropenem MICs.
A Managed Care Organization's Initiative to Improve Patient Safety in the Use of Concentrated Insulin

■■ A Managed Care Organization’s Initiative to Improve Patient Safety in the Use of Concentrated Insulin As obesity becomes more prevalent, so have cases of newly diagnosed diabetes. According to 2008 estimates, more than 18 million American adults have diabetes and 12.4% of them take both insulin and oral medications to treat their disease. Patients on both insulin and oral medications commonly present a therapeutic challenge to clinicians attempting to attain tight glycemic control and hemoglobin A1c levels within a desirable range. Often, this group will develop insulin resistance, defined by Cochran et al. as a situation where a patient requires greater than 200 units of insulin daily. Because of this problem, an increase has occurred in the utilization of concentrated U-500 regular insulin. In 1952, U-500 insulin was introduced to market in the United States as beef regular insulin. Later on, the pork version was approved in 1980, followed by the human version in 1997. From June 2007 to June 2009, utilization rates of human U-500 insulin jumped by 137%, mostly because of increases in the number of obese, clinically insulin-resistant patients with type 2 diabetes. Understanding of the pharmacokinetics and pharmacodynamics of U-500 insulin is increasing along with its use. Cochran et al. described that U-500 has a pharmacokinetic profile similar to that of U-100 neutral protamine Hagedorn (NPH) insulin, with slowed absorption and longer duration than U-100 regular insulin. In addition, although an inverse relationship generally exists between insulin concentration and absorption, the absorption curves for U-500 appear to differ in obese versus nonobese patients and with lower, subtherapeutic doses versus higher, clinically therapeutic doses.
Utilisation du sildénafil dans l'hypertension artérielle pulmonaire du prématuré atteint de dysplasie bronchopulmonaire

INTRODUCTION L’hypertension arterielle pulmonaire (HTAP) est un important facteur de morbimortalite dans l’evolution respiratoire des prematures atteints de dysplasie bronchopulmonaire (DBP). Plusieurs traitements de l’HTAP ont deja fait la preuve de leur efficacite, comme par exemple le monoxyde d’azote inhale (iNO). Le sildenafil (vasodilatateur arteriel) est tres utilise chez l’adulte dans l’HTAP mais il reste controverse chez l’enfant et d’autant plus chez le premature. L’objectif de notre travail est d’etudier l’efficacite et l’innocuite du sildenafil dans l’HTAP du premature atteint de DBP et d’evaluer la possibilite du sevrage du iNO apres l’introduction du sildenafil. MATERIEL ET METHODE Nous avons realise une etude observationnelle descriptive retrospective a partir de la consultation des dossiers medicaux des prematures hospitalises dans les services de reanimation neonatale et neonatologie des CHU de l’Archet 2 a Nice et de la conception a Marseille entre juillet 2009 et septembre 2013. Nous avons realise une analyse de 7 prematures traites par sildenafil pour HTAP en contexte de DBP en etudiant en particulier l’efficacite du sildenafil par l’evolution des echographies cardiaques et la possibilite du sevrage en iNO et son innocuite a partir de l’etude des pressions arterielles. RESULTATS Le degre de prematurite (25,5 SA) et le petit poids de naissance (665g) sont des facteurs de risque d’HTAP en contexte de DBP. La gravite de la DBP (85,7% de DBP severe), la persistance du canal arteriel (100%) et les infections respiratoires en sont les facteurs aggravants. L’amelioration de l’aspect du septum interventriculaire a l’echographie cardiaque au fil du temps est un marqueur de l’efficacite du sildenafil ainsi que la diminution de la FiO2 et l’extubation. Le sevrage du iNO est possible en moyenne en 6 jours apres l’introduction du sildenafil. Sous sildenafil, la pression arterielle baisse systematiquement initialement (de maximum 16mmHg) mais la titration (dose moyenne 3,64 mg/kg/jour) permet de maintenir une stabilite hemodynamique et de rendre le sildenafil sur d’utilisation. Le seul effet secondaire imputable au sildenafil est l’apparition d’un priapisme chez un seul enfant. CONCLUSION Le sildenafil devrait etre plus souvent employe en neonatologie, dans l’indication d’HTAP secondaire a la DBP chez le premature. Devant son efficacite, son innocuite et sa tolerance, son utilisation peut etre proposee en premiere intention ou en relais precoce de l’iNO, tout en restant vigilant sur ses effets hemodynamiques possibles.
A Model for Reduced HIV-1 Viral Load Monitoring in Resource-Limited Settings

Background: Viral load monitoring of antiretroviral therapy in low-income countries is rarely used because of high costs. Reducing the frequency of monitoring may make it financially feasible. Methods: We modeled three testing schemes: reduced viral load monitoring (RVLM) with CD4 count at baseline and viral load testing at 6, 36, and 60 months; United States Department of Health and Human Services (US DHHS) Treatment Guidelines; and World Health Organization (WHO) Guidelines using a cohort of 313 HIV-infected patients using Kaplan-Meier analysis. Results: Median time to detection of antiretroviral therapy (ART) failure using RVLM was 147 days; using US DHHS, it was 115 days; and using WHO guidelines, it was 1110 days. Median time for the development of first thymidine analog mutation was 594 days. The cost of RVLM was significantly lower than US DHHS. Conclusions: RVLM detected failure significantly sooner than CD4 count monitoring alone at a lower cost than US DHHS monitoring. RVLM is a potentially effective method of monitoring ART in resource-limited settings.
Roles of BRCA1 and its interacting proteins

Germline mutations of BRCA1 predispose women to breast and ovarian cancers. BRCA1 contains several functional domains that interact directly or indirectly with a variety of molecules, including tumor suppressors (p53, RB, BRCA2 and ATM), oncogenes (c‐Myc, casein kinase II and E2F), DNA damage repair proteins (RAD50 and RAD51), cell‐cycle regulators (cyclins and cyclin‐dependent kinases), transcriptional activators and repressors (RNA polymerase II, RHA, histone deacetylase complex and CtIP) and others. Mounting evidence indicates that these physical associations are not artifacts; rather, BRCA1 is likely to serve as an important central component in multiple biological pathways that regulate cell‐cycle progression, centrosome duplication, DNA damage repair, cell growth and apoptosis, and transcriptional activation and repression. This review examines our understanding of the significance of the interactions between BRCA1 and other proteins, through which BRCA1 maintains genome integrity and represses tumor formation. BioEssays 22:728–737, 2000. Published 2000 John Wiley & Sons, Inc.
Number of Subunits Comprising the Epithelial Sodium Channel*

The human epithelial sodium channel (hENaC) is a hetero-oligomeric complex composed of three subunits, α, β, and γ. Understanding the structure and function of this channel and its abnormal behavior in disease requires knowledge of the number of subunits that comprise the channel complex. We used freeze-fracture electron microscopy and electrophysiological methods to evaluate the number of subunits in the ENaC complex expressed in Xenopus laevis oocytes. In oocytes expressing wild-type hENaC (α, β, and γ subunits), clusters of particles appeared in the protoplasmic face of the plasma membrane. The total number of particles in the clusters was consistent with the whole-cell amiloride-sensitive current measured in the same cells. The size frequency histogram for the particles in the clusters suggested the presence of an integral membrane protein complex composed of 17 ± 2 transmembrane α-helices. Because each ENaC subunit has two putative transmembrane helices, these data suggest that in the oocyte plasma membrane, the ENaC complex is composed of eight or nine subunits. At high magnification, individual ENaC particles exhibited a near-square geometry. Functional studies using wild-type αβ-hENaC coexpressed with γ-hENaC mutants, which rendered the functional channel differentially sensitive to methanethiosulfonate reagents and cadmium, suggested that the functional channel complex contains more than one γ subunit. These data suggest that functional ENaC consists of eight or nine subunits of which a minimum of two are γ subunits.
Neurological complications in b-thalassemia

Over the years, several reports have demonstrated involvement of the nervous system in b-thalassemia patients. Neurological complications have been attributed to various factors such as chronic hypoxia, bone marrow expansion, iron overload, and desferrioxamine neurotoxicity. In most cases, neurological involvement does not initially present with relevant signs or symptoms (i.e., is subclinical) and can only be detected during neurophysiological or neuroimaging evaluation. Abnormal findings in the visual, auditory, and somatosensory evoked potential recordings are mainly attributed to DFO neurotoxicity. On the other hand, nerve conduction velocity abnormalities are associated either to chronic hypoxia and older age or to hemosiderosis, whether by means of pancreas involvement or not. Neuropsychological studies available reveal a considerably high prevalence of abnormal IQ, not correlating, however, to factors such as hypoxia or iron overload. It is proposed that factors associated to severe chronic illness, rather than the disease per se, could be responsible for these findings. Such factors include regular school absence due to transfusions and frequent hospitalizations, physical and social restrictions resulting from the disease and its treatment, abnormal mental state due to the awareness of being chronically ill, and, last, the overly protective family attitude that leads to restricted initiative and psychosocial development. As life expectancy for b-thalassemia patients extends, the use of neurophysiologic and neuropsychologic monitoring becomes imperative, enabling early detection of neural pathway impairment and allowing for appropriate management, in order to achieve a better life quality for this patient group. 2006 Elsevier B.V. All rights reserved.
Cat scratch disease and lymph node tuberculosis in a colon patient with cancer

A 71-year-old man operated for a sigmoid tumour remained in the surveillance after adjuvant chemotherapy. After 3 years, a left axillary lymph node was visible on CT scan. The biopsy revealed a necrotising and abscessed granulomatous lymphadenitis, suggestive of cat scratch disease. The patient confirmed having been scratched by a cat and the serology for Bartonella henselae was IgM+/IgG−. Direct and culture examinations for tuberculosis were negative. The patient was treated for cat scratch disease. One year later, the CT scan showed increased left axillary lymph nodes and a left pleural effusion. Direct and cultural examinations to exclude tuberculosis were again negative. Interferon-γ release assay testing for tuberculosis was undetermined and then positive. Lymph node and pleural tuberculosis were diagnosed and treated with a good radiological response. This article has provides evidence of the importance of continued search for the right diagnosis and that two diagnoses can happen in the same patient.
Location and User Activity Preference Based Recommendation System

The high utility of Location Based Social Network (LBSN) to infer user spatio temporal activity preference so as to provide personalized context aware Place Of Interest(POI) recommendation and to integrate efficient mobile theft identification system and context aware profile changer. In order to reduce the problem complexity, separately consider the spatial and temporal characteristics of user activity preference in LBSNs. Index Terms—Location based social networks, spatial, temporal, tensor factorization, user activity preference.
0.35um SiGe BiCMOS front-end amplifier for 10Gb/s optical receiver

A 10Gb/s front-end amplifier for optical receiver is realized in a 0.35um SiGe BiCMOS technology. It combines a preamplifier and a limiting amplifier and both are realized with differential architecture. The preamplifier is realized as a transimpedance amplifier with common base input stage. The limiting amplifier consist two stages of Cherry-Hooper architecture. The Simulation shows that the amplifier achieves gain of 102.2dB with constant output swing (450 mVp-p) at a bit rate of 10Gb/s.