INVITRO ANTIOXIDANT STUDY AND SEARCH FOR A NOVEL BIOACTIVE COMPOUND FROM LEAVE FRACTIONS OF CANTHIUM COROMANDELICUM (BURM.F.) ALSTON

Background: Plants are enriched with various types of phytoconstituents present in the form of secondary metabolites that ensure its medicinal value. Keeping this in view, the present investigation was carried out focusing on its antioxidant property that may mainstay of exploring other therapeutic potency. Hence, phytochemical investigation followed with antioxidant property was evaluated in the leaf extracts of the selected plant Canthium coromandelicum (Burm.f.) Alston, collected from eastern coastal belt of south Odisha. Objective: To carry out the phytochemical studies of various leaf extracts and fractions of Canthium coromandelicum (Burm.f.) Alston. In vitro antioxidant activities were also estimated to justify the leaves being used in traditional medicine as an antioxidant. Methods: In vitro antioxidant activity was assayed by DPPH scavenging activity and 2-deoxy-D-ribose degradation. Estimation of total phenolic content and total flavonoid content were done. Further HPTLC finger print of active fractions was performed to isolate and purify the active components presence in them. One of the isolated purified compounds was characterized by various spectral methods. Results: Preliminary phytochemical analysis of leaf extracts confirmed the presence of various phytoconstituents such as flavonoids, terpenoids, saponins, steroids, tannins, quinones and gums. Ethyl acetate fractions of the extract showed potential antioxidant property compared to all the other extracts and fractions. Further, Methanolic extract was found to be rich in phenolic and flavonoid contents compared to the aqueous extract; hence, ethyl acetate fraction of the methanolic extract was chosen for In vitro antioxidant activity. The spectral characterization isolated purified compound was found to be a flavonoid glycoside i.e., Kaempferol 3-O-β-D-glucopyranoside. Conclusion: The present preliminary phytochemical analysis coupled with TPC, TFC and in vitro antioxidant study evaluated the herbal as a potential source of antioxidants with identification of a novel bioactive compound that opens vista to explore its novel bioactive compound which will help for various pharmacological activities. INTRODUCTION: Oxidative free radicals bear responsibility in situations causing health problems like asthma, cancer, cardiovascular diseases, cataract, diabetes, gastrointestinal inflammatory diseases, liver disease, muscular degeneration and other inflammatory processes 1 . QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232.6(9). 3841-52 Article can be accessed online on: www.ijpsr. com DOI link: http://dx.doi. org/10.13040/IJPSR.0975-8232.6(9). 3841-52 These free radicals found in chemical compounds which contain an unpaired electron spinning on the peripheral orbits around the nucleus. The family of free radicals generated from the oxygen is called Reactive Oxygen Species (ROS) causing damage to other molecules by extracting electrons from them in order to attain stability 2 . In normal conditions of cellular metabolism, ROS (Reactive oxygen species) are formed continuously which are scavenged and converted to nonreactive species by different intracellular enzymatic and/or non enzymatic antioxidant system 3 . Excess production or an ineffective elimination of ROS in biological system induce oxidative stress and cause damage to