Miracle-dz/UniFAR-FTUs huggingface dataset:
Characteristics of Anopheles arabiensis Larval Habitats in Tubu Village, Botswana

ABSTRACT: Documented information on the ecology of larval habitats in Botswana is lacking but is critical for larval control programs. Therefore, this study determined the characteristics of these habitats and the influences of biotic and abiotic factors in Tubu village, Botswana. Eight water bodies were sampled between January and December, 2013. The aquatic vegetation and invertebrate species present were characterized. Water parameters measured were turbidity (NTU), conductivity (µS/cm), oxygen (mg/l), and pH. Larval densities of Anopheles arabiensis mosquitoes and their correlation with abiotic factors were determined. Larval breeding was associated with ‘short’ aquatic vegetation, a variety of habitats fed by both rainfall and flood waters and sites with predators and competitors. The monthly mean (± SEmean) larval density was 8.16±1.33. The monthly mean (±SEmean) pH, conductivity, oxygen, and turbidity were 7.65±0.13, 1152.834±69.171, 5.59±1.33, and 323.421±33.801, respectively. There was a significant negative correlation between larval density and conductivity (r = -0.839; p < 0.01), while a significant positive correlation occurred between turbidity and larval density (r = 0.685; p < 0.05). Oxygen (r = 0.140; p > 0.05) and pH (r = 0.252; p > 0.05) were not correlated with larval density. Floods and diversified breeding sites contributed to prolonged and prolific larval breeding. ‘Short’ aquatic vegetation and predator-infested waters offered suitable environments for larval breeding. Turbidity and conductivity were good indicators for potential breeding places and can be used as early warning indices for predicting larval production levels.
An Annotated Checklist of Culicoides Latreille, 1809 (Insecta: Ceratopogonidae: Diptera) with Incorporation of a Vector Species List from India

ABSTRACT: Culicoides Latreille, 1809 (Insecta : Diptera : Ceratopogonidae) are small nematocerous biological vectors of a wide range of pathogens of veterinary and medical importance. They are distributed worldwide but prefer warm, damp, and muddy areas. Female midges require blood for egg maturation. Studies on taxonomy, proper identification keys, and distribution patterns of these flies across different geographical regions of India of these flies are limited. This article provides an updated checklist of Culicoides spp. from India collected from various scattered publications, along with their synonyms and details on their subgenera, geographical distribution, and type locality. A compiled list of different Culicoides vectors from India has also been included separately in this article, along with the type of the diseases spread.
Tick Infestation of Small Mammals in an English Woodland

ABSTRACT: Tick infestations on small mammals were studied from April to November, 2010, in deciduous woodland in southern England in order to determine whether co-infestations with tick stages occurred on small mammals, a key requirement for endemic transmission of tick-borne encephalitis virus (TBEV). A total of 217 small mammals was trapped over 1,760 trap nights. Yellow-necked mice (Apodemus flavicollis) made up the majority (52.5%) of animals, followed by wood mice (A. sylvaticus) 35.5% and bank voles (Myodes glareolus) 12%. A total of 970 ticks was collected from 169 infested animals; 96% of ticks were Ixodes ricinus and 3% I. trianguliceps. Over 98% of ticks were larval stages. Mean infestation intensities of I. ricinus were significantly higher on A. flavicollis (6.53 ± 0.67) than on A. sylvaticus (4.96 ± 0.92) and M. glareolus (3.25 ± 0.53). Infestations with I. ricinus were significantly higher in August than in any other month. Co-infestations with I. ricinus nymphs and larvae were observed on six (3.6%) infested individuals, and fifteen small mammals (8.9%) supported I. ricinus — I. trianguliceps co-infestations. This work contributes further to our understanding of European small mammal hosts that maintain tick populations and their associated pathogens, and indicates that co-infestation of larvae and nymph ticks does occur in lowland UK. The possible implications for transmission of tick-borne encephalitis virus between UK ticks and small mammals are discussed.
Antioxidant activity and high-performance liquid chromatographic analysis of phenolic compounds during in vitro callus culture of Plantago ovata Forsk. and effect of exogenous additives on accumulation of phenolic compounds.

BACKGROUND Plantago ovata, commonly called psyllium, is known to be a rich source of polyphenolic compounds. The present study was aimed at determining polyphenol content and studying their antioxidant activities in P. ovata during in vitro callus culture. An attempt was also made to enhance polyphenol content using external additives. The role of PAL gene in polyphenol accumulation was also studied. RESULTS The study indicated the presence of significant amounts of polyphenols, including flavonoids, in P. ovata callus. A gradual increase in polyphenol and flavonoid content was observed up to the third passage (63 days) of callus culture, which declined at the next passage. The third-passage callus showed highest antioxidant activity. High-performance liquid chromatographic results indicated the presence of high amounts of gallic acid and rutin in P. ovata calli; however, other polyphenols were also present but to a lesser extent. Additive supplementation was effective in enhancing polyphenol production and in increasing antioxidant activity in P. ovata callus. CONCLUSION The present research reported accumulation of polyphenols in callus culture of P. ovata, which could be applied to isolation of polyphenols for various beneficial purposes. It also indicated enhancement in the production of several polyphenols and also an increase in antioxidant activity in the additive-treated callus.
Lung volume during swallowing: single bolus swallows in healthy young adults.

PURPOSE This study examined the relationship between swallowing and lung volume initiation in healthy adults during single swallows of boluses differing in volume and consistency. Differences in lung volume according to respiratory phase surrounding the swallow were also assessed. METHOD Nine men and 11 women between the ages of 19 and 28 years served as study participants. Lung volume and respiratory phase data were recorded as each participant completed 5 trials each of 10-mL and 20-mL water boluses by cup, and thin and thick paste boluses by spoon, presented in randomized order. RESULTS Significant differences in lung volume at swallow initiation were found based on bolus consistency but not on bolus volume. No differences were found for lung volume initiation based on the respiratory phase surrounding the swallow or for the respiratory pattern based on bolus volume or consistency. CONCLUSION Findings of this study extend the existing knowledge base regarding the interaction of the swallow and respiratory systems by identifying targeted lung volumes at swallow initiation. In addition to other swallow-related biomechanical events and respiratory phase relationships surrounding a swallow, the lung volume at swallow initiation may be an important consideration when investigating swallow physiology and physiopathy.
Hepatoprotective effects of soluble rice protein in primary hepatocytes and in mice.

BACKGROUND The production of rice-derived by-products has increased owing to the growing use of processed rice products. The objective of this study was to isolate highly purified proteins from a rice by-product, rice syrup meal, and to examine their hepatoprotective effects in vitro and in vivo. RESULTS Soluble rice protein (SRP70) was obtained via enzymatic processing of rice syrup meal using Termamyl SC and Alcalase. Mass spectrometry analysis showed that SRP70 contained low-molecular-weight (<600 Da) peptides. SRP70 did not affect the viability of rat primary hepatocytes and ameliorated tert-butyl hydroperoxide (t-BHP)-induced cytotoxicity. t-BHP-induced elevations in hepatocyte alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase activities were reduced by SRP70 in a dose-dependent manner. In addition, t-BHP exposure increased the level of malondialdehyde, a toxic reactive aldehyde, which was dose-dependently decreased by SRP70 treatment. These SRP70-induced decreases in biochemical parameters were also observed in vivo in mice. In particular, SRP70 increased the activities of liver antioxidant enzymes in t-BHP-treated mice, including catalase and glutathione peroxidase, as well as increasing the level of glutathione, an antioxidant peptide. SRP70-mediated activation of antioxidant enzymes was shown to be due to the up-regulation in their gene expressions, while nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase 4 (NOX4), a pro-oxidant enzyme, was down-regulated by SRP70. Hematoxylin and eosin staining also showed that SRP70 protected the liver from histopathological changes induced by t-BHP. CONCLUSION Taken together, these data showed that SRP70, which is derived from a rice-processing by-product, had hepatoprotective effects in vitro and in vivo.
Word learning in children with primary language impairment: a meta-analysis.

PURPOSE The present study is a meta-analysis that examines the difference in novel word learning performance between children with primary language impairment (LI) and typically developing children. Participant and task characteristics were examined as variables that potentially moderated children's word learning. METHOD Eight hundred and forty-six published studies were retrieved from conventional databases (PsycINFO, MEDLINE, and Web of Science). Of these studies, 28 met the criteria for inclusion in the meta-analysis, yielding 244 effect sizes across experimental conditions. RESULTS LI groups showed significantly lower word learning performance than typical age-matched groups and equivalent performance to typical language-matched groups. Moderator analyses showed that the magnitude of the group difference relative to age peers was significantly associated with participants' chronological age, receptive language and cognitive abilities, task and novel word type, and the extent of novel word exposure. CONCLUSION The difference in novel word learning performance between children with LI and age-matched children is strongly affected by task and participant characteristics in the primary studies.
MicroRNA‐155 controls Toll‐like receptor 3‐ and hepatitis C virus‐induced immune responses in the liver

The hepatitis C virus (HCV) establishes persistent infections despite strong activation of the innate immune system through TLR3 and other sensors. Therefore, we analysed regulatory mechanisms of TLR3‐induced immune responses in nonparenchymal liver cells (NPCs). Effects of Interleukin‐10 (IL‐10), transforming growth factor beta (TGF‐β) and immunoregulatory miR‐155 on poly I:C‐activated murine (C57BL/6) Kupffer cells (KC) and sinusoidal endothelial cells (LSEC) were assessed in vitro. NPCs were assayed for inflammatory and antiviral cytokines and T‐cell (Balb/c)‐activating factors. Gene expression of miR‐155, IL‐10, TGF‐β and interferon sensitive genes (ISGs) in biopsies of patients with HCV was determined by qrt‐PCR. TLR3‐induced antiviral activity in murine NPCs was potently suppressed by IL‐10 and TGF‐β which correlated with decreased TLR3 expression and inhibition of NF‐κB and IRF‐3 activation. T‐cell activation, induced by TLR3‐activated NPCs, was also suppressed by IL‐10 and TGF‐β, which was associated with a down‐regulation of CD80 and CD86. Pretreatment with IL‐10 or TGF‐β suppressed TLR3‐induced miR‐155 expression, which itself positively regulated poly I:C‐mediated immune responses, thus counteracting IL‐10 or TGF‐β‐induced immunosuppression. In addition, hepatic expression of miR‐155 was elevated in chronically infected patients with HCV, was associated with an IL‐28B SNP (rs12979860) and was inversely correlated with HCV serum load and ISG expression levels. As miR‐155 is a key regulator of anti‐inflammatory mechanisms that control innate and adaptive hepatic immune responses during HCV infection, miR‐155 based therapies may represent a novel mechanism to control HCV in the future.
Examining continuity of early expressive vocabulary development: the generation R study.

PURPOSE The authors investigated continuity and discontinuity of vocabulary skills in a population-based cohort in the Netherlands. METHOD Mothers of 3,759 children completed the Dutch version of the MacArthur Short Form Vocabulary Checklist (Zink & Lejaegere, 2003) at 18 months and a Dutch translation of the Language Development Survey (Rescorla, 1989) at 30 months. At both ages, expressive vocabulary delay was defined as vocabulary scores <10th age- and gender-specific percentile. RESULTS Of the children, 85.2% had normal vocabulary development at both ages, 6.2% were "late bloomers," 6.0% had late onset expressive vocabulary delay, and 2.6% had persistent expressive vocabulary delay. Word production and comprehension at 18 months explained 11.5% of the variance in 30-month vocabulary scores, with low birth weight, child age, gender and ethnicity, maternal age and education, and parenting stress explaining an additional 6.2%. Multinomial logistic regression was used to identify biological, demographic, and psychological factors associated with each of the vocabulary delay outcome groups relative to the typically developing group. CONCLUSIONS Although multiple perinatal, demographic, and maternal psychosocial factors significantly predicted vocabulary skills at 30 months, positive predictive value and sensitivity were low. Future studies should address to what extent additional factors, such as brain maturation and genetic influences, can improve the prediction and understanding of continuity and discontinuity of language delay.
Hepatoprotective studies of a fraction from the fruits of Terminalia belerica roxb. On experimental liver injury in rodents

The effects of an orally administered fraction (TB5) from the fruits of Terminalia belerica in experimental liver injury induced by carbon tetrachloride (CCI4) were studied. Hexobarbitone‐induced sleep, zoxazolamine‐induced paralysis, transaminases, bilirubin, total protein in serum and microsomal lipid peroxidation and triglycerides in liver were used as indices of liver injury.
HBsAg plasma level kinetics: a new role for an old marker as a therapy response predictor in vertically infected children on combination therapy

We aimed to investigate the ability of HBsAg plasma level kinetics to predict therapy response by studying 23 children with infancy‐acquired chronic hepatitis B (CHB) during combination sequential therapy with lead‐in lamivudine (LAM) and add‐on interferon‐α (IFN‐α) [5 responders (R = anti‐HBs seroconversion) and 18 nonresponders (NR)] and to assess their relationship with pretreatment intrahepatic HBV‐DNA and cccDNA and HBsAg and HBcAg liver expression. Plasma HBsAg levels were measured in samples before (treatment week 0 = TW0), during (TW9, TW28, TW52) and after (follow‐up week = FUW24) therapy by Abbott ARCHITECT® assay [log10IU/mL]. Baseline liver HBV‐DNA and cccDNA were quantified by real‐time TaqMan PCR [log10copies/ng genomic DNA]. HBsAg and HBcAg liver expression was evaluated by immunostaining of formalin‐fixed, paraffin‐embedded specimens [number of positive cells/1000 hepatocytes]. All results are presented as medians. Plasma: at baseline, on‐treatment and during follow‐up, HBsAg levels were lower in R than NR (TW0: 4.36 vs 4.75;TW28: 2.44 vs 4.35;TW52: 0 vs 4.08 and FUW24: 0.17 vs 4.35, all P < 0.05). Liver: baseline HBV‐DNA (3.82 vs 4.71, P = 0.16) and cccDNA (1.98 vs 2.26, P = 0.18) tended to be lower in R than NR, HBsAg expression was lower in R than NR (0.5 vs 4.7, P = 0.03), and HBcAg expression was similar between R and NR. There were positive correlations between plasma HBsAg levels and liver HBV‐DNA (r = 0.44, P = 0.04), cccDNA (r = 0.41, P = 0.04) and HBsAg liver expression (r = 0.38, P = 0.05). Lower baseline HBsAg plasma levels, lower HBsAg expression in liver and on‐treatment decline of plasma HBsAg levels heralds HBsAg clearance and response to treatment in tolerant children with CHB.
Incidence of virological failure and emergence of resistance with twice‐daily vs every 8‐h administration of telaprevir in the OPTIMIZE study

The OPTIMIZE study demonstrated noninferior efficacy between telaprevir (TVR) twice daily (bid) vs every 8‐h (q8h) administration. This analysis compared the selective pressure of both dosing regimens by characterisation of the hepatitis C virus (HCV) variants emerging in genotype 1 (G1) HCV‐infected patients who did not achieve sustained virological response (SVR). HCV NS3•4A population sequencing was performed at baseline and time of failure (viral breakthrough, stopping rule or relapse). TVR‐resistant variants were classified by fold change in inhibitory concentration (IC50). Baseline TVR‐resistance was low (<5%) and did not preclude achieving SVR in either arm. The proportion of patients with TVR‐resistant variants at time of failure was similar in the bid (15%) and q8h (17%) dosing arms. The majority of variants and virological failures occurred in G1a patients, and mutations V36M, R155K and R155T (G1a), and V36A, T54A and A156S (G1b) were significantly enriched in both treatment arms. The number and type of emerging TVR‐resistant variants in non‐SVR patients were comparable between treatment arms and were consistent with previous observations. No differences in viral resistance profiles were observed between TVR‐based treatment arms in non‐SVR patients, indicating a similar selective pressure of TVR bid and q8h dosing.
The impact of listening condition on background noise acceptance for young adults with normal hearing.

PURPOSE To evaluate the effect of different speech conditions on background noise acceptance. A total of 23 stimulus pairings, differing in primary talker gender (female, male, conventional), number of background talkers (1, 4, 12), and gender composition of the background noise (female, male, mixed) were used to evaluate background noise acceptance. METHOD A repeated measures research design was used. Participants were 15 female and 15 male young adults with normal hearing. Background noise acceptance was evaluated with the acceptable noise level (ANL). RESULTS The results revealed main effects of primary talker gender, number of background talkers, and listener gender. ANL was lower for conditions of a female primary talker and for conditions with multitalker backgrounds. Male listeners had significantly lower ANLs than female listeners. An interaction occurred between primary talker and background noise composition. For female primary talker conditions, the male talker backgrounds produced the lowest ANLs. For male primary talker conditions, the lowest ANLs were obtained with the female and male background talker conditions. CONCLUSIONS Background noise acceptance depends on the listener and the stimulus condition. Stimulus selection can influence the measurement of ANL. The results support the use of the commercially available ANL materials.
Disparate access to treatment regimens in chronic hepatitis C patients: data from the TRIO network

Despite the clinical success in the real‐world of all oral hepatitis C virus (HCV) therapy with response rates approaching that seen in the clinical trials, access has been limited by many payers with discussion of prioritization of treatment based upon AASLD guidelines. We evaluated patients in the TRIO network who were prescribed sofosbuvir (SOF)‐based regimens to determine reasons for not starting treatment. Trio Health is a disease management company that works in partnership with academic medical centres, community physicians and specialty pharmacies in the United States to optimize care for HCV. Data for 3841 patients prescribed a sofosbuvir‐containing regimen between December 2013 and September 2014 were obtained through this programme. Of the entire group, 315 (8%) patients did not start the prescribed sofosbuvir‐containing therapy. A total of 141 (45%) of the nonstart patients had a commercial plan as their primary insurance, 137 (44%) were primarily covered by Medicaid, 17 (5%) were primarily covered by Medicare, and 20 (6%) were either without coverage or coverage was not specified. Reasons for nonstarts were varied and overlapping. Only 15 patients (5% of nonstarts) did not start because they were unreachable or failed to complete required testing. Another 39 patients who did not start (12%) were following their physicians' direction to either wait for new treatment options or to hold treatment for an unspecified reason. Insurance‐related processes and financial reasons accounted for 254 (81%) of the 315 nonstarts. The remaining 7 (2%) patients did not have a specified reason for not starting treatment. Nonstart rates were highest in the Medicaid‐covered population at 35%. Medicare and Commercial nonstart rates were 2% and 6%, respectively. In a matched comparison, patients with commercial coverage were 6.5 times as likely to start SOF‐based therapy compared to patients with Medicaid. Despite high SVR rates of SOF‐based regimens in clinical practice, there are still barriers to access to care. In fact, almost half of the nonstart patients had advanced fibrosis scores (F3 or F4) and should have been prioritized to start treatment. As better treatment for HCV with high efficacy and low side effect rates become available, the disparity in access to treatment, as evidenced by the high nonstart rate in the Medicaid‐covered group, must be resolved.
Impact of prematurity on language skills at school age.

PURPOSE The existing literature on language outcomes in children born prematurely focuses almost exclusively on standardized test scores rather than discourse-level abilities. The authors of this study looked longitudinally at school-age language outcomes and potential moderating variables for a group of twins born prematurely versus a control group of twins born at full term, analyzing both standardized test results and language sample data from the population-based Western Reserve Reading Project (WRRP; Petrill, Deater-Deckard, Thompson, DeThorne, & Schatschneider, 2006). METHOD Fifty-seven children born prematurely, at ≤32 weeks or <1,500 g, were compared with 57 children born at full term and were matched for age, gender, race, and parental education. Data included discourse-level language samples and standardized test results, collected at average ages 7, 8, and 10 years. The language samples were analyzed to yield a number of semantic and syntactic measures that were consolidated via factor analysis. RESULTS Regression models showed significant differences between the 2 groups for standardized test results, although the mean score for both groups fell in the normal range. For the discourse-level language measures, however, differences never reached statistical significance. Parental education was significantly associated with improved standardized test scores. CONCLUSIONS These findings suggest that in the absence of frank neurological impairment, sophisticated semantic and syntactic skills may be relatively intact in the discourse-level language of children born prematurely. Implications for assessment, particularly the potential role of attention and executive function in standardized testing tasks, are reviewed.