Miracle-dz/UniFAR-FTUs huggingface dataset:
Translesional Synthesis on DNA Templates Containing a Single Abasic Site

Site-specifically modified oligodeoxynucleotides containing a single natural abasic site or a chemically synthesized (tetrahydrofuran or deoxyribitol) model abasic site were used as templates for primer extension reactions catalyzed by the Klenow fragment of Escherichia coli DNA polymerase I or by calf thymus DNA polymerase α. Analysis of the fully extended products of these reactions indicated that both polymerases preferentially incorporate dAMP opposite the natural abasic site and tetrahydrofuran, while DNA templates containing the ring-opened deoxyribitol moiety block translesional synthesis, promoting sequence context-dependent deletions. The frequency of nucleotide insertion opposite the three types of abasic sites follows the order dAMP > dGMP > dCMP > dTMP. The frequency of chain extension was highest when dAMP was positioned opposite a natural abasic site. The frequency of translesional synthesis past abasic sites follows the order tetrahydrofuran > deoxyribose > deoxyribitol. The Klenow fragment promotes blunt end addition of dAMP; this reaction was much less efficient than insertion of dAMP opposite an abasic site. We conclude that the miscoding potential of a natural abasic site in vitro closely resembles that of its tetrahydrofuran analog. Ring-opened abasic sites favor deletions. Studies with polymerase α in vitro predict preferential incorporation of dAMP at abasic sites in mammalian cells.
TP53 Arg72Pro polymorphism and lung cancer risk: A meta‐analysis

No clear consensus has been reached on the TP53 Arg72Pro polymorphism (G12139C) and lung cancer risk. Thus, a meta‐analysis was conducted to summarize the possible association. There was no statistical association between 12139C (Pro allele) and lung cancer risk in Caucasians compared with 12139G allele. However, the association was observed in all subjects (9,387 patients and 9,922 controls, p = 0.04, OR = 1.08, 95% CI 1.00–1.17), as well as in Asians (p = 0.0004, OR = 1.14, 95% CI 1.06–1.22). The association was also found in Asians under recessive genetic model (p < 0.00001, OR = 1.37, 95% CI 1.20–1.57) and homozygote comparison (CC vs. GG) (p < 0.0001, OR = 1.34, 95% CI 1.16–1.56). 12139C allele might increase the lung adenocarcinoma risk compared with 12139G allele (p = 0.01, OR = 1.11, 95% CI 1.02–1.21), and the effect was also found under recessive genetic model (p = 0.003, OR = 1.28, 95% CI 1.09–1.50) and homozygote comparison (CC vs. GG) (p = 0.007, OR = 1.28, 95% CI 1.07–1.52). There was an elevated association between the 12139C and the stage I lung cancer under dominant genetic model (p = 0.04, OR = 1.48, 95% CI 1.02–2.16), but no association was observed in other stages. No association of smoking was found between 12139C allele and lung cancer under recessive genetic model. Our result indicated that 12139C might increase the risk of lung cancer under recessive genetic model in adenocarcinoma, in Asians, and in lung cancer stage I. More studies stratified for lung cancer stage‐genotyping interaction should be performed to clarify the role of TP53 Arg72Pro polymorphism in the development of lung cancer. © 2009 UICC
Expression level of sonic hedgehog correlated with the speed of gastric mucosa regeneration in artificial gastric ulcers

Gastric ulcer healing is a complex process involving cell proliferation and tissue remodeling. Sonic hedgehog (Shh) activates the Shh signaling pathway, which plays a key role in processes such as tissue repair. Shh and interleukin 1β (IL1β) have been reported to influence the proliferation of gastric mucosa. We evaluated the relationships between the speed of gastric ulcer healing and the levels of expression of Shh and IL1β.
Roles of antimicrobial peptides such as defensins in innate and adaptive immunity

A number of antimicrobial peptides such as defensins have multiple functions in host defence. Defensins are produced not only by phagocytic cells and lymphocytes, but also by the epithelial cell lining of the gastrointestinal and genitourinary tracts, the tracheobronchial tree, and keratinocytes. Some are produced constitutively, whereas others are induced by proinflammatory cytokines and exogenous microbial products. Defensins produced by cells in the course of innate host defence serve as signals which initiate, mobilise, and amplify adaptive immune host defences. Administration of defensins with antigens to mice enhances both cellular (Th1-dependent) and humoral (Th2-dependent) cytokine production and immune responses. Linkage of defensins to weak tumour antigens potentiates their immunoadjuvant effects. Defensins use multiple cellular receptors, which endows them with the capacity to marshall adaptive host defences against microbial invaders.
Tkachenko modes of vortex lattices in rapidly rotating bose-einstein condensates.

We calculate the in-plane modes of the vortex lattice in a rotating Bose condensate, from the slowly rotating to mean-field quantum Hall limits. The Tkachenko mode frequency, linear in wave vector k for lattice rotational velocities Omega much smaller than the lowest sound wave frequency in a finite system, becomes quadratic in k in the opposite limit. The system also supports an inertial mode of frequency >or=2omega. The calculated frequencies are in excellent agreement with recent observations of Tkachenko modes by Phys. Rev. Lett. , 91, 100402 (2003)].
A new deferiprone controlled release system obtained by ultrasound-assisted compression

Abstract Objectives: This study implements the design of an innovative dosage form using ultrasound-assisted compression of thermoplastic polymers and the development of controlled release tablets for the oral administration of deferiprone in two doses per day. Methods: Binary matrix tablets containing deferiprone and thermoplastic polymers have been prepared using an ultrasound-assisted tableting machine. Scanning electron microscopy has been employed to determine a sintering phenomenon of the excipients. Water uptake and drug release studies have been carried out to evaluate the ability of the polymers to control the drug release. Results: SEM micrographs showed that some polymers underwent the sintering process and the in vitro dissolution test showed good fit of the release data from these tablets to the zero-order kinetic model. Conclusions: Carbopol 974P and 971P have been selected as matrix forming polymers for the final formulation. The polymer percolation threshold has been exceeded with 15% w/w of polymer. Therefore, sustained release tablets have been developed with only 15% of excipient. This implies that matrix tablets containing 750 mg of API, intended for two administrations a day, can be obtained with a similar weight to those existing in the market containing 500 mg of API for three administrations a day.
A combined ¹H nuclear magnetic resonance and electrospray ionization-mass spectrometry analysis to understand the basal metabolism of plant-pathogenic Fusarium spp.

Many ascomycete Fusarium spp. are plant pathogens that cause disease on both cereal and noncereal hosts. Infection of wheat ears by Fusarium graminearum and F. culmorum typically results in bleaching and a subsequent reduction in grain yield. Also, a large proportion of the harvested grain can be spoiled when the colonizing Fusarium mycelia produce trichothecene mycotoxins, such as deoxynivalenol (DON). In this study, we have explored the intracellular polar metabolome of Fusarium spp. in both toxin-producing and nonproducing conditions in vitro. Four Fusarium spp. , including nine well-characterized wild-type field isolates now used routinely in laboratory experimentation, were explored. A metabolic "triple-fingerprint" was recorded using (1)H nuclear magnetic resonance and direct-injection electrospray ionization-mass spectroscopy in both positive- and negative-ionization modes. These combined metabolomic analyses revealed that this technique is sufficient to resolve different wild-type isolates and different growth conditions. Principal components analysis was able to resolve the four species explored-F. graminearum, F. culmorum, F. pseudograminearum, and F. venenatum-as well as individual isolate differences from the same species. The external nutritional environment was found to have a far greater influence on the metabolome than the genotype of the organism. Conserved responses to DON-inducing medium were evident and included increased abundance of key compatible solutes, such as glycerol and mannitol. In addition, the concentration of γ-aminobutyric acid was elevated, indicating that the cellular nitrogen status may be affected by growth on DON-inducing medium.
Apple MacBook Air

要是在一年多以前，有童鞋问水果本本哪个最鸡肋，相信答案大多会集中到Air上——不光是因为这玩意儿性能低下，更主要的是价格高高在上，低配版本已经上万了。 但是这一切的一切在地主熬夜看完10月21日的水果发布会之后改变——特别是这家伙看到HK官网报价仅为7188港纸，
Improving Safe Sleep Environments for Well Newborns in the Hospital Setting

Objective. Following the “Back to Sleep” campaign, deaths from sudden infant death syndrome (SIDS) were reduced. However, SIDS and sleep-related deaths continue to occur. Studies demonstrate that modeling by health care workers influences parents to place infants supine for sleep. Recently, additional emphasis has been placed on environment. The purpose of this study was to improve sleep position and environment in the hospital. Methods. A Plan-Do-Study-Act cycle was initiated. Sleeping infants were observed at baseline. A bundled intervention was implemented; infants were again observed. Parents were surveyed. Results. At baseline, 25% (36/144) of sleeping infants were safe; the majority of unsafe sleep was a result of environment. Postintervention, significantly more (58%; 145/249) had safe sleep (P < .0001). Most parents planned to use the supine position (95%; 96/101); none planned to cosleep. Many intended to adjust their infants’ home sleep environment. Conclusion. Using a multifaceted approach significantly improved infant safe sleep practice in the hospital setting.
Optimal planning software platform development with cloud computing technology

The paper describes research and development of software platform for optimal operation planning of pulp-and-paper and forestry enterprises using cloud computing technology. This work is a part of long-term activity carried out by Petrozavodsk State University (PetrSU) in the field of scientific research, software development and customer projects for enterprises of pulp-and-paper and forestry industry of Russia. The Platform uses advanced mathematical models and optimization algorithms developed by IT-park of PetrSU to create services for solving a series of planning tasks for different industries: production of corrugated packaging, sawmill, plywood production, timber enterprise.
La capacidad innovadora como determinante del aprendizaje.

En este trabajo se analiza el impacto en forma de aprendizaje y transferencia tecnologica de aquellos proyectos de I+D+i financiados con fondos publicos. Analizando el nivel de aprendizaje y de transferencia tecnologica, el perfil de las empresas con un mayor o menor nivel de aprendizaje. Ademas se analiza la relacion del aprendizaje con el efecto de adicionalidad financiera de las ayudas, es decir, se relaciona el nivel de aprendizaje con el impacto de las ayudas sobre el gasto en I+D de las empresas subvencionadas. La conclusion principal de este trabajo es que las caracteristicas del comportamiento innovador de las empresas estan altamente relacionadas con el nivel de aprendizaje. Resulta que las empresas con un mayor esfuerzo y orientacion innovador consideran los efectos de aprendizaje mucho mas importante que las empresas pocas innovadoras. Ademas se ha detectado un grupo de empresas pequenas y pocas innovadoras que apenas han mejorado su capacidad tecnologica. Parece que para este tipo de empresas el proceso de aprendizaje no esta asegurada debido a la falta de experiencia y una masa critica en un campo tan complejo y especializado como la innovacion.
A new image fusion algorithm based on bayer format

At present, all the algorithms are based on RGB image fusion, and in fact most of the image acquisition and imaging equipment but not the RGB image, a color filter array based on the most common color in the array is the BAYER image format. This paper mainly expands a fusion algorithm based BAYER format. The new algorithm is better than RGB in standard deviation, spatial frequency and information entropy. From comparison of calculation amount, the algorithm of Bayer is less than that of RGB.
Total Iron Binding Capacity and Transferrin Concentration in the Assessment of Iron Status

Abstract Transferrin concentration and total iron binding capacity (TIBC) are currently used to assess iron status. Although correlation between TIBC and transferrin is generally considered as good, conversion factors between the two analytes found in literature show large differences. Although the price per test is lower for TIBC, there are a number of analytical advantages of serum transferrin. Due to binding of iron to other plasma proteins (mainly albumin), TIBC methods generally overestimate the iron binding capacity of transferrin. Moreover, no generic reference values are available for TIBC. In contrast to TIBC, internationally accepted interim reference ranges are available for serum transferrin. The introduction of the international CRM 470 protein standard material has lead to a significant reduction in interlaboratory variation for transferring measurements. In view of these observations, determination of transferrin concentration, rather than TIBC, is recommended. However, in non-European populations characterized by a marked genetic variation in transferrin (TF BC and TF CD variants), in certain cases, immunochemical determination of transferrin may lead to errors. In these populations, TIBC measurements may be preferred.
REVERSIBLE TRABECULAR BONE DENSITY LOSS FOLLOWING INDUCED HYPO‐OESTROGENISM WITH THE GnRH ANALOGUE BUSERELIN IN PREMENOPAUSAL WOMEN

Thirteen premenopausal women who each received 400 μg three times daily of intranasal buserelin for 6 months as treatment for endometriosis participated in this study. Cortical bone mineral content (BMC) in the mid‐shaft of the right femur measured by dual photon densitometry, and trabecular bone density (TBD) in the lumbar vertebrae (L2–4) measured by quantitative computed tomography, were evaluated prior to, at the end of 6 months’ buserelin treatment and after 6 months off treatment. Significant hypo‐oestrogenism (P>0·001) was sustained during the period on treatment. The significant reduction in the mean lumbar vertebral TBD of 5·9% (P>0·02) observed at the end of treatment with buserelin was regained within 6 months following resumption of ovarian activity. There was a marginally significant (P=0·07) reduction of only 0·9% in the mean femoral cortical BMC at the end of treatment. Six months after the end of treatment cortical BMC values were not significantly different from the pretreatment values. There was no significant correlation between the degree of hypo‐oestrogenism and the corresponding change in vertebral TBD, (r=0·20; P>0·10).
Economic and Environmental Generation and Voyage Scheduling of All-Electric Ships

An all-electric ship (AES) uses integrated power generators and an energy storage system (ESS) to match its propulsion and service loads, thus forming an isolated microgrid. Existing work has hitherto set the minimisation of the AES operational cost as the single optimisation objective, with the reduction of the greenhouse gas (GHG) emission merely treated as a constraint of the optimisation. Moreover, the potential of the ESS as part of the scheduling has not been fully exploited. The work presented in this paper emphasises the environmental concerns and makes GHG mitigation a separate objective, thus expanding the optimisation into multi-objective. In achieving both objectives, the ESS-integrated joint generation - voyage scheduling of the AES is proposed: the generation scheduling is combined with the load management (propulsion load - cruising speed) and the ESS dispatch to optimally operate the diesel generators. The optimisation is formulated using optimal control, and is solved using non-dominated sorting genetic algorithm II (NSGA-II). Extensive simulations demonstrate that optimising the cruising speed (voyage) jointly with the generation scheduling results in the reduction of both operational cost and GHG emission, compared to the fixed-voyage generation scheduling. Integrating the ESS dispatch into the generation scheduling further enhances the benefits.
Modulation of Innate Immunity by lignin-Carbohydrate, a Novel TLR4 Ligand, Results in Augmentation of Mucosal IgA and Systemic IgG Production

Previous study revealed that a specific lignin-carbohydrate preparation, named as lignin-rich enzyme lignin (LREL) derived from plant husk, is a novel toll-like receptor 4 ligand and shows a potent immune-stimulatory activity against dendritic cells (DCs) in vitro. In this report, we investigated immune-stimulatory activity of LREL in vivo. Single intraperitoneal (i.p.) or oral treatment of LREL elicited activation of systemic and mucosal DCs, which were accompanied by significant elevation of cell surface activation markers and ratio of IL-12p40 producing cells. In addition, LREL-fed mice showed not only mucosal DCs activation but also significant increase of IFN-γ+ CD4+ T cells in mesenteric lymph node (MLN), respectively. We further examined the effect of LREL oral immunization in combination with ovalbumin (OVA) on the activation of acquired immune system. In LREL administered group, total mucosal IgA concentration was significantly increased, while antigen-specific immunoglobulin A (IgA) concentration was not changed between groups. On the other hand, both total and antigen-specific IgG concentrations in plasma were significantly increased in the LREL administered group. Taken together, oral treatment of LREL is able to affect mucosal and systemic antibodies induction and might be useful for effective immune-stimulatory functional foods and mucosal vaccine adjuvant.
Antenna Systems for the Meteorological Radar Design

Two antenna arrays with linear and circular polarization for meteorological radiosonde tracking radar are compared and discussed. The 26 dB gain antenna operates in 1.765-1.795 GHz range. Both arrays consist of four subarrays. Each subarray comprises suspended rectangular patch antennas fed by air filled strip lines. The 4 element array incorporated into the center of the big array forms wide beam radiation pattern and allows stable balloon tracking at the start moment. Optimal combining both electrical and mechanical scanning allowed to design cheap and reliable meteorological radar
The Response of Paroxysmal Supraventricular Tachycardia to Overdrive Atrial and Ventricular Pacing:

Pacing During Supraventricular Tachycardia. Introduction: Standard electrophysiologic techniques generally allow discrimination among mechanisms of paroxysmal Supraventricular tachycardia. The purpose of this study was to determine whether the response of paroxysmal Supraventricular tachycardia to atrial and ventricular overdrive pacing can help determine the tachycardia mechanism.
A Dataset and Method for Electric Guitar Solo Detection in Rock Music

This paper explores the problem of automatically detecting electric guitar solos in rock music. A baseline study using standard spectral and temporal audio features in conjunction with an SVM classifier is carried out. To improve detection rates, custom features based on predominant pitch and structural segmentation of songs are designed and investigated. The evaluation of different feature combinations suggests that the combination of all features followed by a post-processing step results in the best accuracy. A macro-accuracy of 78.6% with a solo detection precision of 63.3% is observed for the best feature combination. This publication is accompanied by release of an annotated dataset of electric guitar solos to encourage future research in this area.
Organic Nitrates in Heart Failure Revisited: Pentaerythritol Tetranitrate Induces Heme Oxygenase 1 to Protect the Myocardium.

See related article, pp 978–987 Organic nitrates are efficacious drugs for treatment of angina pectoris attacks, acute coronary syndromes, pulmonary edema, and hypertensive crisis. They work by releasing nitric oxide (NO•) or a NO•-related compound (eg, nitrosothiols). Thereby, they activate soluble guanylyl cyclase/cyclic GMP–dependent pathways and lead to antiaggregation and vasodilation (preferentially venodilation) resulting in venous pooling, preload and afterload reduction, reduced left ventricular filling pressures, and reduced myocardial oxygen demand.1 This pharmacodynamic profile promises benefits also for chronic treatment of cardiovascular disease like stable coronary heart disease or chronic heart failure. However, the use of organic nitrates is limited by the rapid development of nitrate tolerance. It is driven mainly by a loss of NO bioavailability caused by increase in reactive oxygen species (ROS) like superoxide (O2•−) originating from activated nicotine amide dinucleotide phosphate oxidases, a dysfunctional mitochondrial respiratory chain, or uncoupled NO synthases.1 Especially O2•− undergoes an avid reaction with NO• to yield peroxynitrite (ONOO− …
Radiobiological studies of a high‐energy modulated proton beam utilizing cultured mammalian cells

The modulated, 160‐MeV proton beam produced by the Harvard Cyclotron has been examined in detail for its ability to kill mammalian cells as assayed by colony forming ability. Using two different cell exposure techniques, the characteristics of position and total dose in producing cell death in two aneuploid cell lines selected for their radiobiological relevance have been determined. The parameters which describe the survival curves after proton or 60Co irradiation show no statistical differences, except on the distal portion of the final Bragg peak of the proton beam, where RBE increases to approximately 1.4. This increase in RBE results in extending the cell killing effect of the beam by approximately 2 mm. This effect may be of practical significance in the irradiation of tissue such as the pituitary where the position of the Bragg peak is of great importance. The overall killing efficiency ratio between the modulated high‐energy proton beam and 60Co γ rays was 1.00 ± . 01 (standard error).
Changing ratios of nuclear estrone to estradiol binding in endometrium at implantation: regulation by chorionic gonadotropin and progesterone during rescue of the primate corpus luteum.

Nuclear binding of 17β-estradiol (NE2R) and estrone (NE1R) was measured in monkey endometrium at different times in the nonfertile menstrual cycle and compared to that during implantation in the fertile menstrual cycle. After finding significant differences in nuclear estrogen binding in the periimplantation interval, we mimicked natural rescue of the corpus luteum by administering either exogenous hCG or progesterone to unmated monkeys during the late luteal phase. In all, endometriectomies were performed on 44 female rhesus monkeys. Besides the 27 unmated monkeys that provided endometrial tissue from the midfoliicular phase through the luteal phase, 9 cycling females were mated on day 12 after the onset of menses, 4 others received hCG injections on cycle day 23, and 4 more received Silastic implants containing progesterone on day 23. Total concentrations of NE1R and NE2R were measured on days 8, 12, 15, 18, and 24 of the menstrual cycle (unmated monkeys) and on the day 24 for both the mated and treated.