Miracle-dz/UniFAR-FTUs huggingface dataset:
Characterization of Human Inducible Costimulator Ligand Expression and Function1

The inducible costimulator (ICOS) is the newest member of the CD28/CD152 receptor family involved in regulating T cell activation. We constructed a soluble-Ig fusion protein of the extracellular domain of human ICOS and used it as a probe to characterize expression patterns of the ICOS ligand (ICOSL). ICOSIg did not bind to CD80- or CD86-transfected Chinese hamster ovary cell lines, demonstrating that ICOSL is distinct from those ligands identified for CD28/CD152. ICOSIg showed selective binding to monocytic and B cell lines, whereas binding was undetectable on unstimulated monocytes and peripheral blood T and B cells. Expression of ICOSL was induced on monocytes after integrin-dependent plastic adhesion. Pretreatment of monocytes with mAb to the β2-integrin subunit CD18 decreased adhesion and abolished ICOSL up-regulation but had no effect on CD80/86 (CD152 ligand (CD152L)) expression. Both ICOSL and CD152L were up-regulated on monocytes by IFN-γ but by distinct signaling pathways. Unlike CD152L expression, ICOSL expression did not change when monocytes were differentiated into dendritic cells (DCs) or after DCs were induced to mature by LPS, TNF-α, or CD40 ligation. Addition of ICOSIg to allogeneic MLRs between DCs and T cells reduced T cell proliferative responses but did so less efficiently than CTLA4Ig (CD152Ig) did. Similarly, ICOSIg also blocked Ag-specific T cell proliferation to tetanus toxoid. Thus, ICOSL, like CD80/86, is expressed on activated monocytes and dendritic cells but is regulated differently and delivers distinct signals to T cells that can be specifically inhibited by ICOSIg.
An approach for parametric and statistical comparisons of similar sensor models

Often in computer-based simulations, sensor models are used to generate realistic data to stimulate an algorithm under test. These algorithms might include, but are not limited to, guidance, navigation, control, tracking, and mission management algorithms. This paper builds on the authors' previous research to apply a systematic approach for comparing two separately developed models of the same sensor. An example application comparing two radar models is used to demonstrate the application of these methods. When working on a particular simulation or project that spans several individuals or contractors it is common for multiple models of the same sensor to be created. Multiple versions of a model may be the result of independent development, a different simulation emphasis, or due to evolution of a model over the course of a development cycle. These models may behave very differently from one another even if they are “drop-in” replacements that accept the same input format and generate the same output format. It is important for the simulation developers as well as the end users to understand the differences between two models so that they may decide to adopt or reject a new version of a model or adequately compare results generated using the two models. This motivates a need for a structured approach for comparing similar models using parametric and statistical techniques. Previous work by the authors focused on the application of design of experiments techniques (DoE) for generating model inputs, in an efficient and effective manner, that span the parameter space over which the model is designed to be valid and for which it will be used in the broader simulation environment. The results described here extend the previous approach to include parametric and statistical model comparison techniques. Monte Carlo simulations are used to generate statistical metrics for comparing the models.
Non-penetrating femtosecond laser intrastromal astigmatic keratotomy in patients with mixed astigmatism after previous refractive surgery.

PURPOSE To report the outcomes of the correction of mixed astigmatism with non-penetrating femtosecond laser intrastromal astigmatic keratotomy in patients with previous refractive surgery. METHODS One hundred twelve eyes that had low mixed astigmatism after excimer laser surgery, refractive lens exchange, or phakic intraocular lens implantation underwent intrastromal astigmatic keratotomy using paired symmetrical non-penetrating intrastromal arcuate keratotomies created 60 μm from the surface to 80% depth at 7 mm diameter. Outcome measures included uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), subjective refraction, and keratometry. A coupling ratio was calculated to assess the change in spherical equivalent. Average follow-up was 7.6 ± 2.9 months. Patients were divided into two groups: no excimer laser corneal ablation and previous excimer laser surgery. Preoperative and postoperative data were compared between groups and analyses were performed on the whole group of eyes. RESULTS Overall, the mean UDVA improved significantly from 0.18 ± 0.14 to 0.02 ± 0.12 logMAR (6/9 to ≈6/6 Snellen) (P < .01). The mean absolute subjective cylinder decreased significantly from 1.20 ± 0.47 diopters (D) preoperatively to 0.55 ± 0.40 D postoperatively (P < .01). Subjective sphere decreased significantly from +0.61 ± 0.33 to +0.17 ± 0.36 D (P < 0.01). The mean CDVA was -0.03 ± 0.08 logMAR (≈ 6/6 Snellen) preoperatively and -0.05 ± 0.09 logMAR (≈ 6/5 Snellen) postoperatively (P = .06). The coupling ratio was 0.92 ± 0.45. There was no statistically significant difference in the preoperative and postoperative sphere, cylinder, UDVA, CDVA, and coupling ratio between groups. No surgical complications occurred. CONCLUSIONS Femtosecond laser intrastromal astigmatic keratotomy was effective at reducing refractive error in patients where other surgical options were exhausted. Predictability and efficacy could be improved with nomogram refinement.
Binding of RFX2 and NF‐Y to the testis‐specific histone H1t promoter may be required for transcriptional activation in primary spermatocytes

The testis‐specific linker histone H1t is transcribed exclusively in pachytene spermatocytes during spermatogenesis. The H1t promoter contains two imperfect inverted repeats which together comprise the X‐box motif that is known to bind the transcription factor regulatory factor X (RFX). Out of all the histone H1 family promoters this motif appears only in the H1t promoter and may contribute to H1t tissue‐specific expression. We show by Western blotting, EMSA, ChIP analysis, and real‐time RT‐PCR that the rat H1t X‐box is bound by RFX2 in vivo in spermatocytes. We demonstrate that transcription factor NF‐Y binds to the CCAAT‐box motif that is located downstream and adjacent to the X‐box and that testis NF‐Y interacts either directly or indirectly with RFX2. Furthermore, we show that both the X‐box and CCAAT‐box are required for promoter activity and that co‐expression of RFX2 greatly enhances testis histone H1t promoter activity in the GC‐1spg germinal cell line. J. Cell. Biochem. 104: 1087–1101, 2008. © 2008 Wiley‐Liss, Inc.
Learning from Bacteria about Natural Information Processing

Under natural growth conditions, bacteria live in complex hierarchical communities. To conduct complex cooperative behaviors, bacteria utilize sophisticated communication to the extent that their chemical language includes semantic and even pragmatic aspects. I describe how complex colony forms (patterns) emerge through the communication‐based interplay between individual bacteria and the colony. Individual cells assume newly co‐generated traits and abilities that are not prestored in the genetic information of the cells, that is, not all the information required for efficient responses to all environmental conditions is stored. To solve newly encountered problems, they assess the problem via collective sensing, recall stored information of past experience, and then execute distributed information processing of the 109–1012 bacteria in the colony—transforming the colony into a “super‐brain. ” I show illuminating examples of swarming intelligence of live bacteria in which they solve optimization problems that are beyond what human beings can solve. This will lead to a discussion about the special nature of bacterial computational principles compared to Turing algorithm computational principles, in particular about the role of distributed information processing.
Plasma cell infiltrates and renal allograft outcomes in indigenous and non‐indigenous people of the Northern Territory of Australia

Introduction: Plasma cell‐rich rejection is a distinct histological phenomenon associated with poor renal allograft outcomes. Aboriginal and Torres Straight Islander (ATSI) transplant recipients have poorer allograft survival and higher rates of acute rejection. We sought to determine whether a higher incidence of plasma cell‐rich infiltrates (PCIR) could account for poorer survival.
Involvement of the "A" isozyme of methyltransferase II and the 29-kilodalton corrinoid protein in methanogenesis from monomethylamine

An assay which allowed detection of proteins involved in the trimethylamine- or monomethylamine (MMA)-dependent methylation of coenzyme M (CoM) was developed. The two activities could be separated by anion-exchange chromatography. The unresolved activity responsible for MMA:CoM methyl transfer eluted from a gel permeation column in the molecular mass range of 32 kDa. The activity was purified to two monomeric proteins of 40 and 29 kDa. The preparation contained protein-bound corrinoid in a mixture of Co(II) and Co(III) states, as well as methyl-B12:CoM methyltransferase (MT2) activity. N-terminal sequence analysis demonstrated that the polypeptides were two previously identified proteins of undefined physiological function. The smaller polypeptide was the monomeric 29-kDa corrinoid protein. The larger polypeptide was the "A" isozyme of MT2. Individually purified preparations of both proteins increased the rate of MMA-dependent CoM methylation by approximately 1.7 mumol/min/mg of purified protein above background activity in the extract of methanol-grown cells. These results indicate that the 29-kDa corrinoid protein and the "A" isozyme of MT2 function in methanogenesis from MMA. A likely mechanism is that the 29-kDa corrinoid is methylated by MMA and the methyl group is then transferred by the "A" isozyme of MT2 to CoM.
A Shared Memory MPP from Cray Research

The CRAY T3D system is the first massively parallel processor from Cray Research. The implementation entailed the design of system software, hardware, languages, and tools. A study of representative applications influenced these designs. The paper focuses on the programming model, the physically distributed, logically shared memory interconnect, and the integration of Digital's DECchip 21064 Alpha AXP microprocessor in this interconnect. Additional topics include latency-hiding and synchronization hardware, libraries, operating system, and tools
Visual acuity measurements in a national sample of British elderly people

BACKGROUND Despite the fact that visual function has an important role in the quality of life in later years, very few studies have measured visual acuity in population based nationwide samples of British elderly people. Such measurements were carried out in the context of the national diet and nutrition survey of people aged 65 years or over (NDNS). METHODS NDNS participants, who were living in 80 different randomly selected postcode areas of mainland Britain, were visited at their home by a nurse who measured visual acuity at 3 metres, using the Glasgow acuity card (GAC) method. In addition, a brief questionnaire related to ocular health was administered. RESULTS Visual acuity was measured in 1362 NDNS participants who were not classified as mentally impaired. Visual impairment (using the WHO low vision criteria) was measured in 195 (14.3%) subjects. Prevalence of visual impairment increased significantly with age (65–74 years 3.1%; 75–84 years 11.6%; 85+ years 35.5%, p<0.001 for trend). Impaired vision was more common in subjects living in a nursing home (odds ratio adjusted for age 2.59 (95% CI 2.23 to 2.96)) and in women (odds ratio adjusted for age 1.55 (95% CI 1.21 to 1.89)). 132 (9.7%) subjects had previously undergone cataract surgery and another 157 (11.5%) had been told that they currently had cataract. Vision improved 0.2 log units or more (at least one Snellen line) with the aid of a pinhole occluder in 289 subjects (21.2%). CONCLUSION Results of this nationwide, community based study confirm that problems with poor distance visual acuity exist in a substantial part of the elderly community, particularly in women and people living in nursing homes.
Physical activity and fitness: Pathways from childhood to adulthood

Contemporary thinking in public health posits that childhood and adolescent physical activity and physical fitness may influence health status during childhood and adolescence, as well as throughout adulthood. This paper addresses the evidence dealing with potential associations of physical activity and physical fitness in childhood and adolescence to health status during childhood, adolescence, and adulthood. There are generally low to moderate relationships between childhood and adolescent physical activity and health (operationalized largely in terms of health‐related physical fitness) and adult physical activity and health. A large part of the variability in health‐related fitness is not accounted for by physical activity as assessed in the available studies. Nevertheless, the trends emphasize the importance of a lifestyle of regular physical activity during childhood and adolescence, which continues into and throughout adulthood, for the health and well‐being of the individuals and populations. Am. J. Hum. Biol. 13:162–172, 2001. © 2001 Wiley‐Liss, Inc.
Two-Dimensional Vector Wind Fields from Volume Imaging Lidar Data

Abstract Spatially resolved wind fields are derived by cross correlation of aerosol backscatter data from horizontal and vertical scans of the University of Wisconsin volume imaging lidar during the 1997/98 Lake-Induced Convection Experiment. Data from three cases are analyzed. The first two cases occurred on 10 and 13 January 1998 during cold-air outbreaks. Horizontal scans at 5 m above the lake reveal cellular structure of the steam fog. Vector winds are derived with 250-m spatial resolution over 60 and 36 km2 areas. These wind fields show acceleration and veering of offshore flow in the convective internal boundary layer along the upwind edge of Lake Michigan. The wind fields are used to compute divergence and vorticity. Effects of shoreline shape and topography are evident in the data. Horizontal wind speeds derived from vertical scans show the effects of convection on the vertical distribution of momentum. In the third case, 21 December 1997, a well-defined, shallow density current flowing offshore a.
Family and child-care provider influences on preschool children's fruit, juice, and vegetable consumption.

Children's intakes of fruit, juice, and vegetables (FJV) do not meet the recommended minimum of five daily servings, placing them at increased risk for development of cancer and other diseases. Because children's food preferences and practices are initiated early in life (e.g., 2-5 years of age), early dietary intervention programs may have immediate nutritional benefit, as well as reduce chronic disease risk when learned healthful habits and preferences are carried into adulthood. Families and child-care settings are important social environments within which food-related behaviors among young children are developed. FJV preferences, the primary predictor of FJV consumption in children, are influenced by availability, variety, and repeated exposure. Caregivers (parents and child-care providers) can influence children's eating practices by controlling availability and accessibility of foods, meal structure, food modeling, food socialization practices, and food-related parenting style. Much remains to be learned about how these influences and practices affect the development of FJV preferences and consumption early in life.
[Immunoregulation and short-term therapeutic effects of super-selective intra-arterial chemotherapy combined with traditional Chinese drugs on gastric cancer patients].

OBJECTIVE To investigate the immunoregulation and short-term therapeutic effects of super-selective intra-arterial chemotherapy combined with Fuzheng Kang'ai Granules, a compound Chinese herbal medicine, on patients with late gastric cancer. METHODS Forty patients with late gastric antrum cancer were randomly divided into study group and control group. Patients in the study group were orally administered Fuzheng Kang'ai Granules 48 hours after the first super-selective left gastric artery chemotherapy with high-dose drugs (EAP regime: VP(16) 100 mg/m(2) + epirubicin 60 mg/m(2) + carboplatin 200 mg/m(2)), while patients in the control group were only administered the same local artery chemotherapy as in the study group. RESULTS The short-term therapeutic efficacy in the study group and control group were 82.5% and 57.5% respectively (P<0.01). The occurrence rates of side effects in the study group were significantly lower than those in the control group (P<0.01). The Karnofsky score of life quality of the study group was obviously higher than that of the control group after treatment (P<0.05). The half survival time and one year survival rate in the study group and control group were (24.9 +/- 1.36) month, 70% (28/40) and (13.7 +/- 0.72) month, 35% (14/40) respectively. They were significantly improved in the study group compared with the control group (P<0.01). The levels of cytokines interleukin 2 (IL-2), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma) after treatment in the study group were significantly higher than those before treatment (P<0.05 or P<0.01), as well as than those after treatment in the control group (P<0.01). On the other hand, the level of immune inhibitory factor soluble interleukin-2 receptor (sIL-2R) after treatment in the study group was lower than that before treatment, as well as than that after treatment in the control group (P<0.01). CONCLUSIONS The super-selective intra-arterial chemotherapy combined with Fuzheng Kang'ai Granules has good short-term therapeutic efficiency and few side effects for patients with late gastric antrum cancer. It can significantly improve the life quality, extend the survival period, and improve the survival rate in patients with late gastric antrum cancer, which may be due to the up-regulation of the immune regulating factors such as IL-2, TNF-alpha, IFN-gamma and down-regulation of the immune inhibitory factor sIL-2R.
A Technique for Differential Noise Figure Measurement of Differential LNAs

This paper presents an approach to measure the noise figure of a differential low-noise amplifier (LNA) based on familiar ldquocold-hotrdquo single-ended noise figure measurements. To demonstrate the usefulness of this approach, measurement results are presented for a wideband differential LNA designed to be used as the first stage of the receiver in the Square Kilometre Array radio telescope. The presented LNA achieves less than 0.41 dB of differential noise figure in the 700 MHz to 1.4 GHz band, differential S11 <-13 dB, differential S21 between 18 and 14 dB, single-ended output P1 dB of -8.2 dBm, and output IP3 of -1 dBm while consuming 81 mA from a 1.3-V supply. The approach of measuring the differential noise figure may be automated with one switch at the output of a standard noise source and one switch at the input to a standard noise figure analyzer or a noise figure meter, allowing for automated noise figure measurements of differential LNAs based on the differential pair topology.
Comparison of the Josephson Voltage Standards of the NIMT and the BIPM (part of the ongoing BIPM key comparison BIPM.EM-K10.b)

A comparison of the Josephson array voltage standards of the Bureau International des Poids et Mesures (BIPM) and the National Institute of Metrology - (Thailand), NIMT, was carried out in November 2015 at the level of 10 V. For this exercise, options A and B of the BIPM.EM-K10.b comparison protocol were applied. Option B required the BIPM to provide a reference voltage for measurement by the NIMT using its Josephson standard with its own measuring device. Option A required the NIMT to provide a reference voltage with its Josephson voltage standard for measurement by the BIPM using an analogue nanovoltmeter and associated measurement loop. In all cases the BIPM array was kept floating from ground. The final results were in good agreement within the combined relative standard uncertainty of 2.6 parts in 1010 for the nominal voltage of 10 V. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm. org/. The final report has been peer-reviewed and approved for publication by the CCEM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).
Blast Injury in a Civilian Trauma Setting is Associated with a Delay in Diagnosis of Traumatic Brain Injury

High-pressure waves (blast) account for the majority of combat injuries and are becoming increasingly common in terrorist attacks. To our knowledge, there are no data evaluating the epidemiology of blast injury in a domestic nonterrorist setting. Data were analyzed retrospectively on patients admitted with any type of blast injury over a 10-year period at a busy urban trauma center. Injuries were classified by etiology of explosion and anatomical location. Eighty-nine cases of blast injury were identified in 57,392 patients (0.2%) treated over the study period. The majority of patients were male (78%) with a mean age of 40 ± 17 years. The mean Injury Severity Score was 13 ± 11 with an admission Trauma and Injury Severity Score of 0.9 ± 0.2 and Revised Trauma Score of 7.5 ± 0.8. The mean intensive care unit and hospital length of stay was 2 ± 7 days and 4.6 ± 10 days, respectively, with an overall mortality rate of 4.5 per cent. Private dwelling explosion [n = 31 (35%)] was the most common etiology followed by industrial pressure blast [n = 20 (22%)], industrial gas explosion [n = 16 (18%)], military training-related explosion [n = 15 (17%)], home explosive device [n = 8 (9%)], and fireworks explosion [n = 1 (1%)]. Maxillofacial injuries were the most common injury (n = 78) followed by upper extremity orthopedic (n = 29), head injury (n = 32), abdominal (n = 30), lower extremity orthopedic (n = 29), and thoracic (n = 19). The majority of patients with head injury [28 of 32 (88%)] presented with a Glasgow Coma Scale score of 15. CT scans on admission were initially positive for brain injury in 14 of 28 patients (50%). Seven patients (25%) who did not have a CT scan on admission had a CT performed later in their hospital course as a result of mental status change and were positive for traumatic brain injury (TBI). Three patients (11%) had a negative admission CT with a subsequently positive CT for TBI over the next 48 hours. The remaining four patients (14%) were diagnosed with skull fractures. All patients (n = 4) with an admission Glasgow Coma Scale score of less than 8 died from diffuse axonal injury. Blast injury is a complicated disease process, which may evolve over time, particularly with TBI. The missed injury rate for TBI in patients with a Glasgow Coma Scale score of 15 was 36 per cent. More studies are needed in the area of blast injury to better understand this disease process.