Miracle-dz/UniFAR-FTUs huggingface dataset:
Design of Self-help Pickup System Based on Single Chip Microcomputer

Designed a self-service pickup system. The core control chip of the system is STC89C52, which contains a GSM module, a keyboard input module, a screen display, and a storage cabinet for relay simulation. The process of the express cabinet can be briefly described as follows: The courier enters the recipient's number and places the package, and the system automatically generates a 6-digit receiving password, which is sent to the recipient's mobile phone through the GSM module. This design can break through the traditional pickup method and save manpower.
Primer hallazgo de Pseudorasbora parva (Temminck & Schlegel, 1846) (Cyprinidae) en la Cuenca Sur de España

First record of Pseudorasbora parva (Temminck & Schlegel, 1846) (Cyprinidae) in the Cuenca Sur of Spain Pseudorasbora parva Temminck & Schlegel (Cyprinidae, subfamily Gobioninae), an invasive Asian fish, has been found in the Cuenca Sur (Hozgarganta and Guadiaro rivers) for the first time thanks to the field works for the assessment of native aquatic fauna and for the early detection of invasive species. These works are being conducting by the Environmental Council (Regional Government of Andalusia). In this work, potential introduction routes and associated biological impacts on native fish species are evaluated, and suggestions for the design of future management strategies are presented. It is expected that P. parva will disperse through other neighbor watercourses and basins. There might be also a risk of transmission of two alien parasites (Sphaerotecum destruens y Anguillicola crassus) to the populations of native fish species. The introduction route for P. parva in the Cuenca Sur is unknown. However, it is likely that the species was first introduced as forage fish to promote angling species such as Micropterus salmoides ,a lready present in the rivers, and thus invade connected water bodies. Eradication or control through direct population management seems unfeasible. Therefore, future management measures focused on monitoring and management of native fish populations are proposed as well as the development of awareness campaigns involving all the stakeholders.
Plasmacytoid dendritic cells cross-prime naive CD8 T cells by transferring antigen to conventional dendritic cells through exosomes

Significance Whether and how pDCs cross-prime CD8 T cells in vivo remain controversial despite extensive studies. Using a vaccine model where antigens were only delivered to pDCs, this report demonstrated that pDCs induced cross-priming and durable CD8 T cell immunity in vivo. However, cross-presenting pDCs required cDCs to achieve cross-priming by transferring antigens to cDCs. cDC1s but not cDC2s played a critical role in pDC-mediated cross-priming, despite both subsets acquiring antigens from pDCs similarly. Antigen transfer from pDCs to bystander cDCs was mediated by pDC-derived exosomes (pDCexos), which were generated under various conditions. Importantly, these pDCexos required cDCs to prime CD8 T cells, similarly to cross-presenting pDCs, thus identifying the pDCexo/cDCs pathway as a mechanism for pDCs to achieve cross-priming. Although plasmacytoid dendritic cells (pDCs) have been shown to play a critical role in generating viral immunity and promoting tolerance to suppress antitumor immunity, whether and how pDCs cross-prime CD8 T cells in vivo remain controversial. Using a pDC-targeted vaccine model to deliver antigens specifically to pDCs, we have demonstrated that pDC-targeted vaccination led to strong cross-priming and durable CD8 T cell immunity. Surprisingly, cross-presenting pDCs required conventional DCs (cDCs) to achieve cross-priming in vivo by transferring antigens to cDCs. Taking advantage of an in vitro system where only pDCs had access to antigens, we further demonstrated that cross-presenting pDCs were unable to efficiently prime CD8 T cells by themselves, but conferred antigen-naive cDCs the capability of cross-priming CD8 T cells by transferring antigens to cDCs. Although both cDC1s and cDC2s exhibited similar efficiency in acquiring antigens from pDCs, cDC1s but not cDC2s were required for cross-priming upon pDC-targeted vaccination, suggesting that cDC1s played a critical role in pDC-mediated cross-priming independent of their function in antigen presentation. Antigen transfer from pDCs to cDCs was mediated by previously unreported pDC-derived exosomes (pDCexos), that were also produced by pDCs under various conditions. Importantly, all these pDCexos primed naive antigen-specific CD8 T cells only in the presence of bystander cDCs, similarly to cross-presenting pDCs, thus identifying pDCexo-mediated antigen transfer to cDCs as a mechanism for pDCs to achieve cross-priming. In summary, our data suggest that pDCs employ a unique mechanism of pDCexo-mediated antigen transfer to cDCs for cross-priming.
Controlled Optimal Black Start Procedures in Smart Grids for Service Restoration in Presence of Electrical Storage Systems

This paper presents an optimisation problem to determine the optimal reclosure order of remotely operable switches deployed in a smart grid consisting in a distribution network equipped with one or more Energy Storage Systems (ESS). The proposed solution integrates nonlinear real and reactive power flow equations, by reconducting them to a set of conic constraints, together with several network operator requirements, such as network radiality and ampacity limits. A numerical simulation validates the approach and concludes the work.
Aspirin and Growth of Small Unruptured Intracranial Aneurysm

Background and Purpose: The role of aspirin in unruptured intracranial aneurysm (UIA) growth remains largely unknown. We aim to identify whether aspirin is associated with a lower rate of UIA growth in patients with UIA <7 mm. Methods: This prospective cohort study consecutively enrolled patients with UIAs <7 mm with ischemic cerebrovascular disease between January 2016 and December 2019. Baseline and follow-up patient information, including the use of aspirin and blood pressure level, were recorded. Patients were considered aspirin users if they took aspirin, including standard- and low-dose aspirin, ≥3× per week. The primary end point was aneurysm growth in any direction or an indisputable change in aneurysm shape. Results: Among the 315 enrolled patients, 272 patients (86.3%) underwent imaging examinations during follow-up (mean follow-up time, 19.6±12.7 months). A total of 113 patients were continuously treated with aspirin. UIA growth occurred in 31 (11.4%) patients. In the multivariate Cox analysis, specific aneurysm locations (anterior communicating artery, posterior communicating artery, or middle cerebral artery; hazard ratio, 2.89 [95% CI, 1.22–6.88]; P=0.016) and a UIA size of 5 to <7 mm (hazard ratio, 7.61 [95% CI, 3.02–19.22]; P<0.001) were associated with a high risk of UIA growth, whereas aspirin and well-controlled blood pressure were associated with a low risk of UIA growth (hazard ratio, 0.29 [95% CI, 0.11–0.77]; P=0.013 and hazard ratio, 0.25 [95% CI, 0.10–0.66]; P=0.005, respectively). The cumulative annual growth rates were as high as 40.0 and 53.3 per 100 person-years in the high-risk patients (>1 risk factor) with and without aspirin, respectively. Conclusions: Aspirin therapy and well-controlled blood pressure are associated with a low risk of UIA growth; the incidence of UIA growth in high-risk patients in the first year is high, warranting intensive surveillance in this patient group. Registration: URL: https://www.clinicaltrials. gov. Unique identifier: NCT02846259.
Inclusive Leadership and Pro-Social Rule Breaking: The Role of Psychological Safety, Leadership Identification and Leader-Member Exchange

This study explores the impact of inclusive leadership on employees’ pro-social rule breaking through the dual path of psychological safety and leadership identification, as well as the moderating role of leader-member exchange in the above paths. We used questionnaires to survey 303 employees at two time points. The results show that inclusive leadership has a significant positive impact on employees’ pro-social rule breaking, that psychological safety and leadership identification play mediating roles in this relationship, and that there is no significant difference between the mediating effects. Leader-member exchange moderates the indirect effect of inclusive leadership on pro-social rule breaking through psychological safety. That is, under a high level of leader-member exchange, inclusive leadership has a strong impact on psychological safety and pro-social rule breaking, but the moderating effect of inclusive leadership on employees’ pro-social rule breaking through leadership identification is not significant. This study provides guidance for improving the vitality of employees and promoting the development of enterprise rules.
Ever and repeat HIV testing rates among male and female clients: Findings from a reproductive health services integration project in South Africa.

BACKGROUND HIV testing rates have increased in recent years. Repeat testing is recommended to identify and treat new HIV infections timeously. However, there are limited data on repeat HIV testing, especially in South Africa (SA). OBJECTIVES To provide data on repeat HIV testing rates in males and females in a district in SA. METHODS A sexual and reproductive health (SRH) service integration model was implemented in seven healthcare facilities in eThekwini District, KwaZulu-Natal Province, SA, between 2009 and 2011. HIV testing data were collected from male and female clients attending these facilities, prior to (baseline) and after the implementation (endline) of the 3-year health services integration intervention. RESULTS There were 230 clients at baseline (195 female, 35 male) and 200 at endline (169 female, 31 male). High ever-tested rates were reported at baseline (females 95.4%, males 74.3%) and endline (females 91.7%, males 87.1%), with large increases in male testing rates over time. In addition, high increases were seen between baseline and endline among those who had tested more than once and more than twice in their lifetime. Increases between baseline and endline testing rates were highest in HIV testing services (HTS) (37.0 - 93.3% for clients who had tested more than once, and 11.1 - 53.3% for those who had tested more than twice). CONCLUSIONS HIV testing and repeat testing increased over time, especially in males and in HTS. Promotion and integration of SRH services are critical to facilitate improved health-seeking behaviour and HIV testing of both male and female clients. They are also important for continued access to HTS at multiple service delivery points.
Sub-10 nm High-k Dielectric SOI-FinFET for HighPerformance Low Power Applications

In this work, sub-10 nm high-k dielectric Silicon-on-Insulator (SOI) FinFET has been investigated for high-performance analog applications with a very low power supply. The performance analysis of 9 nm gate length high-k SOI-FinFET has been compared with conventional FinFET. It has been evaluated that the proposed device provides better solutions in terms of better subthreshold slope, lowering of threshold voltage, device efficiency, and higher switching ratio (Ion/Ioff ratio). Thus, the high-k dielectric SOI-FinFET device paves the way for high-performance switching applications.
Co-Microencapsulation of Islets and MSC CellSaics, Mosaic-Like Aggregates of MSCs and Recombinant Peptide Pieces, and Therapeutic Effects of Their Subcutaneous Transplantation on Diabetes

The subcutaneous transplantation of microencapsulated islets has been extensively studied as a therapeutic approach for type I diabetes. However, due to the lower vascular density and strong inflammatory response in the subcutaneous area, there have been few reports of successfully normalized blood glucose levels. To address this issue, we developed mosaic-like aggregates comprised of mesenchymal stem cells (MSCs) and recombinant peptide pieces called MSC CellSaics, which provide a continuous release of angiogenic factors and anti-inflammatory cytokines. Our previous report revealed that the diabetes of immunodeficient diabetic model mice was reversed by the subcutaneous co-transplantation of the MSC CellSaics and rat islets. In this study, we focused on the development of immune-isolating microcapsules to co-encapsulate the MSC CellSaics and rat islets, and their therapeutic efficiency via subcutaneous transplantation into immunocompetent diabetic model mice. As blood glucose level was monitored for 28 days following transplantation, the normalization rate of the new immuno-isolating microcapsules was confirmed to be significantly higher than those of the microcapsules without the MSC CellSaics, and the MSC CellSaics transplanted outside the microcapsules (p < 0.01). Furthermore, the number of islets required for the treatment was reduced. In the stained sections, a larger number/area of blood vessels was observed around the new immuno-isolating microcapsules, which suggests that angiogenic factors secreted by the MSC CellSaics through the microcapsules function locally for their enhanced efficacy.
Modeling soft robotic actuators using data-driven model reduction

Soft robotics is a emerging research area in the field of robotics with promising application areas where traditional robots fails to perform efficiently. However obtaining a nearly accurate mathematical model of the soft robot is challenging due to its high flexibility and non-linear material properties. The limited information of the system in terms of its parameters have led to alternative modeling approaches. With large scale data collection in terms of evolution of states and system identification, data-driven techniques are favorable alternative to obtain nearly accurate models. Considering the large scale dimensional problem of the resulting model, model reduction technique have been applied to obtain optimal reduced set of governing equations. In this work, the dynamic mode decomposition based system identification technique have been implemented in the soft robot model. This technique does not need any information regarding the parameters of the system and is solely dependent on the measurement of the system states. The obtained dynamic model is a linear approximation of the full-order system. The paper describes the model reduction technique aligned with the soft robotic actuator application.
Controllability and observability robustness of perturbed linear time invariant systems

The controllability and observability of a continuous linear time-invariant system (LTI) under perturbations are analyzed in this paper. Based on various mathematical tools, sufficient conditions to ensure controllability of LTI systems are presented. Besides, the perturbed system corresponds to a perturbation of the matrices, and the conditions that have been established are intimately linked to the structure of perturbed system. By duality, the robustness of observability is also ensured under suitable conditions.
Dispensable Amino Acids, except Glutamine and Proline, Are Ideal Nitrogen Sources for Protein Synthesis in the Presence of Adequate Indispensable Amino Acids in Adult Men.

BACKGROUND Nutritionally, there is a dietary requirement for indispensable amino acids (IAAs) but also a requirement for nitrogen (N) intake for the de novo synthesis of the dispensable amino acids (DAAs). It has been suggested that there might be a dietary requirement for specific DAAs. OBJECTIVES Experiment 1 tested whether 9 of the DAAs (Ala, Arg, Asn, Asp, Gln, Glu, Gly, Pro, Ser) are ideal N sources using the indicator amino acid oxidation (IAAO) technique. Experiment 2 examined whether there is a dietary requirement for Glu in adult men. METHODS Seven healthy men (aged 20-24 y) participated in 11 or 2 test diet intakes, in experiment 1 and 2, respectively, in a repeated measures design. In experiment 1, a base diet consisting of the IAA provided at the RDA was compared with test intakes with the base diet plus addition of individual DAAs to meet a 50:50 ratio of IAA:DAA on an N basis. In experiment 2, the diets corresponded to the amino acid pattern present in egg protein, in which all Glu and Gln was present as Glu, or removed, with Ser used to make the diets isonitrogenous. On each study day the IAAO protocol with l-[1-13C]phenylalanine was used to measure whole-body protein synthesis. RESULTS In experiment 1, repeated measures ANOVA with post hoc multiple comparisons showed that 7 of the 9 DAAs (Ala, Arg, Asn, Asp, Glu, Gly, Ser) decreased IAAO significantly (P < 0.05) compared with base IAA diet, the exceptions being Gln and Pro. In experiment 2, a paired t test did not find significant (P > 0.05) differences in the IAAO in response to removal and replacement of Glu intake. CONCLUSIONS The results suggest that in healthy men most DAAs are ideal N sources for protein synthesis, in the presence of adequate IAAs, and that endogenous synthesis of Glu is sufficient.Registered clinicaltrials.gov identifier: NCT02009917.
Nonlinear stability analysis for an anticipation-memory car following model in the era of autonomous and connected vehicles

Utilizing vehicles' previous transmitted motion information through information communication technologies (ICT) wirelessly an anticipation-memory (AM) car following model is derived to investigate the effects of anticipating driving behaviour on vehicles' position, velocity, acceleration. The changes of space headway, velocity differences, and memory of driving to the preceding vehicle are presented as a parameters of driving anticipation in autonomous and connected vehicles (ACV) environment. The linear stability is carried out to study the qualitative properties of the AM model. The linear and nonlinear analysis support our newly developed model to illustrate the anticipation driving parameter based on previous motion information as feedback of driving in V2V environment which can effectively enhance the traffic flow stability, and the capacity of roads. All results depict that the AM model can potentially decrease the velocity fluctuations in a traffic flow system comparing to previous existing models.
Transmitter Design for Downlink Non-Orthogonal Multiple Access System in Frequency-Selective Channels

In this paper, we propose design methods of timedomain filters at a base station for downlink non-orthogonal multiple access in frequency-selective channels. The filters are determined by solving a signal-to-noise-plus-interference ratio maximization problem under a constraint on intersymbol interference suppression. Furthermore, we propose a power allocation method that maximizes the sum-capacity. Simulation results are provided to show the effectiveness of the proposed method and the superiority to a conventional orthogonal multiple access system.
Classification of Thyroid Tumors Based on Mass Spectrometry Imaging of Tissue Microarrays; a Single-Pixel Approach

The primary diagnosis of thyroid tumors based on histopathological patterns can be ambiguous in some cases, so proper classification of thyroid diseases might be improved if molecular biomarkers support cytological and histological assessment. In this work, tissue microarrays representative for major types of thyroid malignancies—papillary thyroid cancer (classical and follicular variant), follicular thyroid cancer, anaplastic thyroid cancer, and medullary thyroid cancer—and benign thyroid follicular adenoma and normal thyroid were analyzed by mass spectrometry imaging (MSI), and then different computation approaches were implemented to test the suitability of the registered profiles of tryptic peptides for tumor classification. Molecular similarity among all seven types of thyroid specimens was estimated, and multicomponent classifiers were built for sample classification using individual MSI spectra that corresponded to small clusters of cells. Moreover, MSI components showing the most significant differences in abundance between the compared types of tissues detected and their putative identity were established by annotation with fragments of proteins identified by liquid chromatography-tandem mass spectrometry in corresponding tissue lysates. In general, high accuracy of sample classification was associated with low inter-tissue similarity index and a high number of components with significant differences in abundance between the tissues. Particularly, high molecular similarity was noted between three types of tumors with follicular morphology (adenoma, follicular cancer, and follicular variant of papillary cancer), whose differentiation represented the major classification problem in our dataset. However, low level of the intra-tissue heterogeneity increased the accuracy of classification despite high inter-tissue similarity (which was exemplified by normal thyroid and benign adenoma). We compared classifiers based on all detected MSI components (n = 1536) and the subset of the most abundant components (n = 147). Despite relatively higher contribution of components with significantly different abundance and lower overall inter-tissue similarity in the latter case, the precision of classification was generally higher using all MSI components. Moreover, the classification model based on individual spectra (a single-pixel approach) outperformed the model based on mean spectra of tissue cores. Our result confirmed the high feasibility of MSI-based approaches to multi-class detection of cancer types and proved the good performance of sample classification based on individual spectra (molecular image pixels) that overcame problems related to small amounts of heterogeneous material, which limit the applicability of classical proteomics.
Generation and Characterization of Cytochrome P450 2J3/10 CRISPR/Cas9 Knockout Rat Model

Cytochrome P450 2J2 (CYP2J2) enzyme attracts more attention because it not only metabolizes clinical drugs but also mediates the biotransformation of important endogenous substances and the regulation of physiologic function. Although CYP2J2 is very important, few animal models are available to study its function in vivo. In particular, a CYP2J gene knockout (KO) rat model for drug metabolism and pharmacokinetics is not available. In this report, the CRISPR/Cas9 technology was used to delete rat CYP2J3/10, the orthologous genes of CYP2J2 in humans. The CYP2J3/10 KO rats were viable and fertile and showed no off-target effect. Compared with wild-type (WT) rats, the mRNA and protein expression of CYP2J3/10 in liver, small intestine, and heart of KO rats were completely absent. At the same time, CYP2J4 mRNA expression and protein expression were significantly decreased in these tissues. Further in vitro and in vivo metabolic studies of astemizole, a typical substrate of CYP2J, indicated that CYP2J was functionally inactive in KO rats. The heart function indexes of WT and KO rats were also measured and compared. The myocardial enzymes, including creatine kinase–muscle brain type (CK-MB), creatine kinase (CK), and CK-MB/CK ratio, of KO rats increased by nearly 140%, 80%, and 60%, respectively. In conclusion, this study successfully developed a new CYP2J3/10 KO rat model, which is a useful tool to study the function of CYP2J in drug metabolism and cardiovascular disease. SIGNIFICANCE STATEMENT Human CYP2J2 is involved not only in clinical drug metabolism but also in the biotransformation of important endogenous substances. Therefore, it is very important to construct new animal models to study its function in vivo. This study successfully developed a new CYP2J knockout rat model by using CRISPR/Cas9 technology. This rat model provides a useful tool to study the role of CYP2J in drug metabolism and diseases.