Miracle-dz/UniFAR-FTUs huggingface dataset:
lncRNA mortal obligate RNA transcript was downregulated in ovarian carcinoma and inhibits cancer cell proliferation by downregulating miRNA‐21

microRNA‐21 (miRNA‐21) is a well‐characterized oncogenic miRNA in human cancers. In the present study, we found that miRNA‐21 was upregulated, while long noncoding RNA Mortal Obligate RNA Transcript (lncRNA MORT), which has been reported to be silenced in 16 types of cancers, was downregulated in tumor tissues than in adjacent healthy tissues of patients with ovarian carcinoma. Expression of lncRNA MORT in tumor tissues was found to be significantly affected by tumor size but not by tumor metastasis. Expression levels of lncRNA MORT and miRNA‐21 were significantly and inversely correlated in both tumor tissues and adjacent healthy tissues. Overexpression of lncRNA MORT inhibited miRNA‐21, while miRNA‐21 overexpression failed to significantly affect lncRNA MORT expression. Overexpression of lncRNA MORT inhibited, while miRNA‐21 overexpression promoted the proliferation of cells of ovarian cancer cell lines. In addition, miRNA‐21 overexpression partially reversed the inhibitory effects of lncRNA MORT overexpression on cancer cell proliferation. However, overexpression of lncRNA MORT showed no significant effects on cancer cell migration and invasion. Therefore, lncRNA MORT was downregulated in ovarian carcinoma and lncRNA MORT overexpression inhibited cancer cell proliferation, possibly by downregulating miRNA‐21.
NMR solution structure of tricyclo-DNA containing duplexes: insight into enhanced thermal stability and nuclease resistance

Abstract Tc-DNA is a conformationally constrained oligonucleotide analogue which shows significant increase in thermal stability when hybridized with RNA, DNA or tc-DNA. Remarkably, recent studies revealed that tc-DNA antisense oligonucleotides (AO) hold great promise for the treatment of Duchenne muscular dystrophy and spinal muscular atrophy. To date, no high-resolution structural data is available for fully modified tc-DNA duplexes and little is known about the origins of their enhanced thermal stability. Here, we report the structures of a fully modified tc-DNA oligonucleotide paired with either complementary RNA, DNA or tc-DNA. All three investigated duplexes maintain a right-handed helical structure with Watson-Crick base pairing and overall geometry intermediate between A- and B-type, but closer to A-type structures. All sugars of the tc-DNA and RNA residues adopt a North conformation whereas the DNA deoxyribose are found in a South-East-North conformation equilibrium. The conformation of the tc-DNA strand in the three determined structures is nearly identical and despite the different nature and local geometry of the complementary strand, the overall structures of the examined duplexes are very similar suggesting that the tc-DNA strand dominates the duplex structure.
New Concepts in Polypoidal Choroidal Vasculopathy Imaging: A Focus on Optical Coherence Tomography and Optical Coherence Tomography Angiography

Abstract: Polypoidal choroidal vasculopathy (PCV) is a variant of neovascular age‐related macular degeneration. It is characterized by polypoidal dilatations at the terminus of branching vascular network located beneath the retinal pigment epithelium. These polypoidal lesions are best visualized on indocyanine green angiography. With recent advances in ocular imaging, optical coherence tomography (OCT) and OCT angiography (OCTA) have been increasingly used to aid in the diagnosis and monitoring of treatment responses in PCV. This review provides a summary of the current status of various imaging modalities in PCV, with special focus on OCT and OCTA.
Improved Severe Weather Forecasts Using LEO and GEO Satellite Soundings

It is shown here that improvements in numerical weather prediction (NWP) model forecasts of hazardous weather can be obtained by assimilating profile retrievals obtained in real time from combined direct broadcast system (DBS) polar satellite hyperspectral and geostationary satellite multispectral radiance data. Results of NWP model forecasts are shown for two recent tornado outbreak cases: 1) the 3 March 2019 tornado outbreak over the southeast United States and 2) the tornado outbreak that occurred across Illinois, Indiana, and Ohio during the night of 27 May and the morning of 28 May 2019, and 3) the 4 March 2019 severe precipitation event that occurred in southeast China. Improvements in both quantitative precipitation forecasts (QPFs) and predictions of the location of tornado occurrence are obtained. It is also shown that geostationary satellite hyperspectral soundings [i.e.,Fengyun-4A(FY-4A) Geosynchronous Interferometric Infrared Sounder (GIIRS)] further improve hazardous precipitation forecasts when used, in addition to the combined polar hyperspectral and geostationary multispectral satellite profile data, to initialize the numerical forecast model. The lowest false alarm rate (FAR) and the highest probability of detection (POD) and critical success index (CSI) scores are achieved when assimilating atmospheric profile retrievals obtained by combining all the available satellite high-vertical-resolution hyperspectral radiance measurements with geostationary satellite high-spatial-resolution and high-temporal-resolution multispectral radiance measurements.
The Distribution and Possible Roles of Small Cardioactive Peptide in the Nudibranch Melibe leonina

Synopsis The neuropeptide small cardioactive peptide (SCP) plays an integrative role in exciting various motor programs involved in feeding and locomotion in a number of gastropod species. In this study, immunohistochemistry, using monoclonal antibodies against SCPB, was used to localize SCPB-like-immunoreactive neurons in the central nervous system, and map their connections to various tissues, in the nudibranch, Melibe leonina. Approximately 28–36 SCPB-like-immunoreactive neurons were identified in the M. leonina brain, as well as one large neuron in each of the buccal ganglia. The neuropil of the pedal ganglia contained the most SCPB-like-immunoreactive varicosities, although only a small portion of these were due to SCPB-like-immunoreactive neurons in the same ganglion. This suggests that much of the SCPB-like immunoreactivity in the neuropil of the pedal ganglia was from neurons in other ganglia that projected through the pedal–pedal connectives or the connectives from the cerebral and pleural ganglia. We also observed extensive SCPB innervation along the length of the esophagus. Therefore, we investigated the impact of SCPB on locomotion in intact animals, as well as peristaltic contractions of the isolated esophagus. Injection of intact animals with SCPB at night led to a significant increase in crawling and swimming, compared to control animals injected with saline. Furthermore, perfusion of isolated brains with SCPB initiated expression of the swim motor program. Application of SCPB to the isolated quiescent esophagus initiated rhythmic peristaltic contractions, and this occurred in preparations both with and without the buccal ganglia being attached. All these data, taken together, suggest that SCPB could be released at night to arouse animals and enhance the expression of both feeding and swimming motor programs in M. leonina.
Explosive Volcanic Eruptions and Spinodal Decomposition: A Different Approach to Deciphering the Tiny Bubble Paradox

Bubbles in magmas drive explosive volcanic eruptions. The spatial distribution of bubble nucleation sites in an ascending, decompressing, and supersaturating magma is one of the primary controls on ash morphologies and volcanic hazards. The mechanism of bubble formation is important because it ultimately determines the spatial distribution of bubbles in the fragmenting magma. The initial nucleation of bubbles in a homogeneous magma is problematical because excessive surface tension pressure in very small, nascent bubbles should drive exsolved volatiles back into the melt. This thermodynamic barrier to bubble viability confounds understanding of homogeneous bubble nucleation, yet very small bubbles form, grow, and ultimately drive explosive volcanic eruptions. We refer to this as “the tiny bubble paradox. ” Classical nucleation theory typically explains bubble formation and growth, but we propose that a spectrum of bubble‐forming mechanisms may include both homogeneous nucleation and spinodal decomposition (the spontaneous unmixing of phases by uphill diffusion) as end‐member processes. As spinodal decomposition progresses, regularly sized and regularly spaced quasi‐spherical zones form with increasingly high concentration of dissolved water at the centers. Bubble formation occurs as the concentration of water in the interior of the water‐rich zones approaches 100% and the concentration of melt approaches zero. The presence of a broad, diffuse, concentration gradient of water rather than a narrow water‐melt interface means that there is no surface, per se, for surface tension to arise. This is the crux of the solution of the tiny bubble paradox.
PGPR Modulation of Secondary Metabolites in Tomato Infested with Spodoptera litura

The preceding climate change demonstrates overwintering of pathogens that lead to increased incidence of insects and pest attack. Integration of ecological and physiological/molecular approaches are imperative to encounter pathogen attack in order to enhance crop yield. The present study aimed to evaluate the effects of two plant growth promoting rhizobacteria (Bacillus endophyticus and Pseudomonas aeruginosa) on the plant physiology and production of the secondary metabolites in tomato plants infested with Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). The surface sterilized seeds of tomato were inoculated with plant growth promoting rhizobacteria (PGPR) for 3–4 h prior to sowing. Tomato leaves at 6 to 7 branching stage were infested with S. litura at the larval stage of 2nd instar. Identification of secondary metabolites and phytohormones were made from tomato leaves using thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) and fourier-transform infrared spectroscopy (FTIR). Infestation with S. litura significantly decreased plant growth and yield. The PGPR inoculations alleviated the adverse effects of insect infestation on plant growth and fruit yield. An increased level of protein, proline and sugar contents and enhanced activity of superoxide dismutase (SOD) was noticed in infected tomato plants associated with PGPR. Moreover, p-kaempferol, rutin, caffeic acid, p-coumaric acid and flavonoid glycoside were also detected in PGPR inoculated infested plants. The FTIR spectra of the infected leaf samples pre-treated with PGPR revealed the presence of aldehyde. Additionally, significant amounts of indole-3-acetic acid (IAA), salicylic acid (SA) and abscisic acid (ABA) were detected in the leaf samples. From the present results, we conclude that PGPR can promote growth and yield of tomatoes under attack and help the host plant to combat infestation via modulation in IAA, SA, ABA and other secondary metabolites.
Porous Surface Films With Tunable Morphologies and Hydrophobic Properties Based on Block Copolymer Under the Effects of Thermal Annealing

The fabrication of porous-structured polymer films with patterned surface structures has recently attracted increased interest within the material science field. In this work, a series of microstructure scale patterned polymer films were obtained via breath figure methods (BF) with hydrophobic surface films based on self-assembled diblock copolymers by atom-transfer radical polymerization (ATRP). The surface characteristics and morphological properties, pore size, roughness, thickness, and wettability of the block copolymer films was studied in response to variation of the hydrophilic co-monomer structures. A significant improvement of the quality and order of the hydrophobic films was observed in response to thermal annealing and a consequent optimization of the assembly process.
Fate of Mycotoxins During Processing of Foodstuffs I-Aflatoxin B1 During Making of Egyptian Bread.

Fate of aflatoxin B1 during making of Egyptian bread from whole wheat flour spiked with aflatoxin Bl (0.1 μg/g) was investigated. Determinations were made by reversed-phase high pressure liquid chromatography (HPLC) after formation of aflatoxin B2a with trifluoroacetic acid. An average of 19% of the toxin added was destroyed after preparation of fermented dough, whereas subsequent baking (350°C, 2 min) reduced the amount of toxin by an additional 36% to give a total average destruction of 55%. Thus risks remain in preparing Egyptian bread from aflatoxin-contaminated wheat flour.
Identification of Motor Symptoms Related to Parkinson Disease Using Motion-Tracking Sensors at Home (KÄVELI): Protocol for an Observational Case-Control Study

Background Clinical characterization of motion in patients with Parkinson disease (PD) is challenging: symptom progression, suitability of medication, and level of independence in the home environment can vary across time and patients. Appointments at the neurological outpatient clinic provide a limited understanding of the overall situation. In order to follow up these variations, longer-term measurements performed outside of the clinic setting could help optimize and personalize therapies. Several wearable sensors have been used to estimate the severity of symptoms in PD; however, longitudinal recordings, even for a short duration of a few days, are rare. Home recordings have the potential benefit of providing a more thorough and objective follow-up of the disease while providing more information about the possible need to change medications or consider invasive treatments. Objective The primary objective of this study is to collect a dataset for developing methods to detect PD-related symptoms that are visible in walking patterns at home. The movement data are collected continuously and remotely at home during the normal lives of patients with PD as well as controls. The secondary objective is to use the dataset to study whether the registered medication intakes can be identified from the collected movement data by looking for and analyzing short-term changes in walking patterns. Methods This paper described the protocol for an observational case-control study that measures activity using three different devices: (1) a smartphone with a built-in accelerometer, gyroscope, and phone orientation sensor, (2) a Movesense smart sensor to measure movement data from the wrist, and (3) a Forciot smart insole to measure the forces applied on the feet. The measurements are first collected during the appointment at the clinic conducted by a trained clinical physiotherapist. Subsequently, the subjects wear the smartphone at home for 3 consecutive days. Wrist and insole sensors are not used in the home recordings. Results Data collection began in March 2018. Subject recruitment and data collection will continue in spring 2019. The intended sample size was 150 subjects. In 2018, we collected a sample of 103 subjects, 66 of whom were diagnosed with PD. Conclusions This study aims to produce an extensive movement-sensor dataset recorded from patients with PD in various phases of the disease as well as from a group of control subjects for effective and impactful comparison studies. The study also aims to develop data analysis methods to monitor PD symptoms and the effects of medication intake during normal life and outside of the clinic setting. Further applications of these methods may include using them as tools for health care professionals to monitor PD remotely and applying them to other movement disorders. Trial Registration ClinicalTrials.gov NCT03366558; https://clinicaltrials.gov/ct2/show/NCT03366558 International Registered Report Identifier (IRRID) DERR1-10.2196/12808
Green Development in the Construction of Family Houses in Urban and Rural Settlements in Slovakia

Measures that have been implemented to promote green development and environmental protection are increasingly affecting the construction of family houses and hence the economic and social growth. The representatives of the Europe Regional Network of World Green Building Council claim that the building emissions in Europe create up to 36% of the total carbon dioxide production. Therefore, the application of ecological building materials can be one of the possible ways to reach equilibrium between the social-economic growth and green development. The main objective of this paper was to find out the approach of people in urban and rural settlements in Slovakia towards the question of green development in terms of selection of building material for the construction of a family house and their economic-social situation. The issue was mapped in Slovakia using the empirical survey in the form of a questionnaire. The research was evaluated using statistical hypothesis testing methods, descriptive statistics methods, and data visualization. The main economic and social coordinates were identified that influence the choice of building materials for the construction of houses. The results led to conclusions that extend the current knowledge in the field of green development and sustainability in connection with the construction of family houses and the economic–social question in Slovakia. Results indicated that ecological materials are underestimated at nearly 71%. The identified reason for the research is, besides the economic and social issue, mainly the low level of information and promotion of ecological materials that can be used for the construction of family houses, as well as the low level of support by the state of those who are interested in the construction of ecological houses. The following research perspectives in this area should focus even more deeply on the synchronization of ecological, economic, and social aspects of sustainability, not only in the construction of family houses from ecological materials in Slovakia, but also in the construction of public and other buildings in urban and rural settlements.
Targeting Myeloid-Derived Cells: New Frontiers in the Treatment of Non-alcoholic and Alcoholic Liver Disease

Non-alcoholic fatty liver disease (NAFLD) and Alcoholic Liver Disease (ALD) are major causes of liver-related morbidity and mortality and constitute important causes of liver transplantation. The spectrum of the liver disease is wide and includes isolated steatosis, steatohepatitis, and cirrhosis. The treatment of NAFLD and ALD remains, however, an unmet need, and therefore it is a public health priority to develop effective treatments for these diseases. Alcoholic and non-alcoholic liver disease share common complex pathogenetic pathways that involve different organs and systems beyond the liver, including the gut, the adipose tissue, and the immune system, which cross-talk to generate damage. Myeloid-derived cells have been widely studied in the setting of NAFLD and ALD and are implicated at different levels in the onset and progression of this disease. Among these cells, monocytes and macrophages have been found to be involved in the induction of inflammation and in the progression to fibrosis, both in animal models and clinical studies and they have become interesting potential targets for the treatment of both NAFLD and ALD. The different mechanisms by which these cells can be targeted include modulation of Kupffer cell activation, monocyte recruitment in the liver and macrophage polarization and differentiation. Evidence from preclinical studies and clinical trials (some of them already in phase II and III) have shown encouraging results in ameliorating steatohepatitis, fibrosis, and the metabolic profile, individuating promising candidates for the pharmacological treatment of these diseases. The currently available results of myeloid-derived cells targeted treatments in NAFLD and ALD are covered in this review.
The economic complexity of US metropolitan areas

ABSTRACT We calculate measures of economic complexity for US metropolitan areas for the period 1998–2015 based on employment data. We show that the concept translates well to the regional setting and to local and traded industries. Large cities and the Northeast have the highest complexity, while most traded industries are more complex than most local ones. In cross-section, metropolitan complexity is associated with higher incomes, though to a lesser extent recently than in the past. However, within-city increases in complexity from year to year are associated with income decreases. Our findings highlight the need for caution when interpreting the relationship between complexity and socioeconomic outcomes.
The liability of mimicry: Implementing “global human resource management standards” in United States and Indian subsidiaries of a South Korean multinational enterprise

There is increasing evidence that multinational enterprises (MNEs) from less dominant economies tend to mimic and disseminate HRM practices sourced from a dominant economy, usually the USA, to overcome their ‘liabilities of origin’. However, our understanding of the specific challenges involved in the implementation of such practices by firms across different national and subsidiary contexts remains limited. Drawing on evidence from a case study of a South Korean MNE, we examine the extent and ways in which global HRM policies mimicking US practices are implemented across its sales, manufacturing, and research and development subsidiaries in the USA and India. We find discernible differences in the implementation of the global policies both between the two host country sites and across the three function-specific subsidiaries in each country, identifying a range of national and subsidiary-specific factors that inform these variable implementation outcomes. In addition to legitimacy challenges related to the source, appropriateness, and process of transfer, we note a unique form of legitimacy challenge - ‘the liability of mimicry’ - whereby local actors can challenge head office policies on the basis of a claim to superior expertise in the dominant practices, as a particular concern of MNEs from emerging economies.
Developing a Grounded Theory on Adaptation After Lung Transplantation From Intermediate-Term Patient Experiences

Background: Previous research revealed that it is possible for lung recipients to experience health 1 year posttransplant, despite not being fully recovered. However, an in-depth, long-term perspective on how lung recipients’ health transition evolves over time is lacking. Thus, the aim of this study was to further develop a grounded theory of health transition by exploring the process of change 1 to 3 years after lung transplantation. Methods: The grounded theory method was used prospectively to analyze the narratives of 14 adult lung recipients who were included at their 1-year follow-up and reinterviewed 2 years later. Results: This novel study contributes an in-depth understanding of the adaptation process after lung transplantation. The greatest concern in the 3 years after lung transplantation was adaptation to a new normality, which was achieved by 3 main strategies: compare, accept, and adjust. Adaptation to a new normality involved understanding that one’s previous life no longer exists and that a new way of living requires adaptation. Successful adaptation resulted in the experience of health and well-being, whereas too many symptoms and limitations in everyday life led to difficulties and a profound sense of illness. Conclusions: Lung recipients can experience health, despite symptoms and complications by adapting to a new normality. This individual process begins posttransplant and continues throughout life.