Miracle-dz/UniFAR-FTUs huggingface dataset:
Negative Input-Resistance Compensator for a Constant Power Load

A negative input-resistance compensator is designed to stabilize a power electronic brushless dc motor drive with constant power-load characteristics. The strategy is to feed a portion of the changes in the dc-link voltage into the current control loop to modify the system input impedance in the midfrequency range and thereby to damp the input filter. The design process of the compensator and the selection of parameters are described. The impact of the compensator is examined on the motor-controller performance, and finally, the effectiveness of the controller is verified by simulation and experimental testing.
Biomechanical determinants of maximal stair climbing capacity in healthy elderly women

Stair walking is an important functional movement task that may require considerable amounts of muscle strength/power. This study aimed to perform a descriptive biomechanical analysis of maximal stair ascent in elderly women and to examine the relationship between mechanical muscle function and maximal stair ascending velocity (MAV). Seventeen healthy elderly women (age 72.4 ± 6.4) were tested for MAV, maximal multi‐joint counter movement jumping (CMJ), and maximal single‐joint isokinetic/isometric muscle moment. Peak knee joint power during MAV was the single independent parameter that explained most of the variation in MAV (50%), however, combining knee and ankle parameters in a multiple regression analysis mean joint power explained 82.4% of the variation in MAV. Generally, multi‐joint CMJ parameters showed stronger correlations with MAV than single‐joint isokinetic/isometric muscle strength parameters. MAV appeared to be highly dependent upon knee and ankle power and to a lesser extent on joint moment and range of motion. Furthermore, CMJ assessment seemed well applicable in healthy elderly individuals to distinguish between differentiated levels of maximal stair walking capacity.
Functional Mapping of Receptor Specificity Domains of Glial Cell Line-derived Neurotrophic Factor (GDNF) Family Ligands and Production of GFRα1 RET-specific Agonists*

The glial cell line-derived neurotrophic factor (GDNF) family ligands (GFLs) (GDNF, neurturin, artemin, and persephin) are critical regulators of neurodevelopment and support the survival of midbrain dopaminergic and spinal motor neurons in vitro and in animal disease models making them attractive therapeutic candidates for treatment of neurodegenerative diseases. The GFLs signal through a multicomponent receptor complex comprised of a high affinity binding component (GDNF-family receptor α-component (GFRα1-GFRα4)) and the receptor tyrosine kinase RET. To begin characterization of GFL receptor specificity at the molecular level, we performed comprehensive homologue-scanning mutagenesis of GDNF, the prototypical member of the GFLs. Replacing short segments of GDNF with the homologous segments from persephin (PSPN) (which cannot bind or activate GFRα1·RET or GFRα2·RET) identified sites along the second finger of GDNF critical for activating the GFRα1·RET and GFRα2·RET receptor complexes. Furthermore, introduction of these regions from GDNF, neurturin, or artemin into PSPN demonstrated that they are sufficient for activating GFRα1·RET, but additional determinants are required for interaction with the other GFRαs. This difference in the molecular basis of GFL-GFRα specificity allowed the production of GFRα1·RET-specific agonists and provides a foundation for understanding of GFL-GFRα·RET signaling at the molecular level.
Precipitation extreme changes exceeding moisture content increases in MIROC and IPCC climate models

Precipitation extreme changes are often assumed to scale with, or are constrained by, the change in atmospheric moisture content. Studies have generally confirmed the scaling based on moisture content for the midlatitudes but identified deviations for the tropics. In fact half of the twelve selected Intergovernmental Panel on Climate Change (IPCC) models exhibit increases faster than the climatological-mean precipitable water change for high percentiles of tropical daily precipitation, albeit with significant intermodel scatter. Decomposition of the precipitation extreme changes reveals that the variations among models can be attributed primarily to the differences in the upward velocity. Both the amplitude and vertical profile of vertical motion are found to affect precipitation extremes. A recently proposed scaling that incorporates these dynamical effects can capture the basic features of precipitation changes in both the tropics and midlatitudes. In particular, the increases in tropical precipitation extremes significantly exceed the precipitable water change in Model for Interdisciplinary Research on Climate (MIROC), a coupled general circulation model with the highest resolution among IPCC climate models whose precipitation characteristics have been shown to reasonably match those of observations. The expected intensification of tropical disturbances points to the possibility of precipitation extreme increases beyond the moisture content increase as is found in MIROC and some of IPCC models.
Aeolian dust in Colorado Plateau soils: Nutrient inputs and recent change in source

Aeolian dust (windblown silt and clay) is an important component in arid-land ecosystems because it may contribute to soil formation and furnish essential nutrients. Few geologic surfaces, however, have been characterized with respect to dust-accumulation history and resultant nutrient enrichment. We have developed a combination of methods to identify the presence of aeolian dust in arid regions and to evaluate the roles of this dust in ecosystem processes. Unconsolidated sandy sediment on isolated surfaces in the Canyonlands region of the Colorado Plateau differs greatly in mineralogical and chemical composition from associated bedrock, mainly aeolian sandstone. Detrital magnetite in the surficial deposits produces moderately high values of magnetic susceptibility, but magnetite is absent in nearby bedrock. A component of the surficial deposits must be aeolian to account for the abundance of magnetite, which formed originally in far-distant igneous rocks. Particle-size analysis suggests that the aeolian dust component is typically as much as 20–30%. Dust inputs have enriched the sediments in many elements, including P, Mg, Na, K, and Mo, as well as Ca, at sites where bedrock lacks calcite cement. Soil-surface biologic crusts are effective dust traps that apparently record a change in dust sources over the past several decades. Some of the recently fallen dust may result from human disturbance of land surfaces that are far from the Canyonlands, such as the Mojave Desert. Some land-use practices in the study area have the potential to deplete soil fertility by means of wind-erosion removal of aeolian silt.
Induction of Neutralizing Antibodies and Th1-Polarized and CD4-Independent CD8+ T-Cell Responses following Delivery of Human Immunodeficiency Virus Type 1 Tat Protein by Recombinant Adenylate Cyclase of Bordetella pertussis

ABSTRACT HIV-Tat, a conserved protein playing a key role in the early life cycle of the human immunodeficiency virus (HIV) has been proposed as a potential AIDS vaccine. An HIV-Tat-based vaccine should elicit a broad, long-lasting, and neutralizing immune response. We have previously demonstrated that the adenylate cyclase (CyaA) from Bordetella pertussis targets dendritic cells and delivers CD8+ and CD4+ T-cell epitopes into the major histocompatibility complex class I and class II presentation pathways. We have also showed that CyaA induced specific and protective cytotoxic T cell responses in vivo. Here, we designed a prototype vaccine based on the HIV type 1 Tat delivered by CyaA (CyaA-E5-Tat) and tested its capacity to induce HIV-Tat-specific cellular as well as antibody responses. We showed that immunization of mice by CyaA-E5-Tat in the absence of adjuvant elicited strong and long-lasting neutralizing anti-Tat antibody responses more efficient than those obtained after immunization with Tat toxoid in aluminum hydroxide adjuvant. Analyses of the anti-Tat immunoglobulin G isotypes and the cytokine pattern showed that CyaA-E5-Tat induced a Th1-polarized immune response in contrast to the Th2-polarized immune responses obtained with the Tat toxoid. In addition, our data demonstrated that HIV-Tat-specific gamma interferon-producing CD8+ T cells were generated after vaccination with CyaA-E5-Tat in a CD4+ T-cell-independent manner. Based on these findings, CyaA-E5-Tat represents an attractive vaccine candidate for both preventive and therapeutic vaccination involving CyaA as an efficient nonreplicative vector for protein delivery.
3D tomography of cells in micro-channels

We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far been limited to steady specimen, we overcome this restriction and present a method to access the 3D shape of moving objects. The key of our principle is a tilted arrangement of the micro-channel with respect to the focal plane of the microscope. This forces cells to traverse the focal plane in an inclined manner. As a consequence, individual layers of passing cells are recorded which can then be assembled to obtain the volumetric representation. The full 3D information allows for a detailed comparisons with theoretical and numerical predictions unfeasible with e.g.\ 2D imaging. Our technique is exemplified by studying flowing red blood cells in a micro-channel reflecting the conditions prevailing in the microvasculature. We observe two very different types of shapes: `croissants' and `slippers'. Additionally, we perform 3D numerical simulations of our experiment to confirm the observations. Since 3D confocal imaging of cells in flow has not yet been realized, we see high potential in the field of flow cytometry where cell classification thus far mostly relies on 1D scattering and fluorescence signals.
Risk of postpartum depressive symptoms with elevated corticotropin-releasing hormone in human pregnancy.

CONTEXT Postpartum depression (PPD) is common and has serious implications for the mother and her newborn infant. A possible link between placental corticotropin-releasing hormone (pCRH) and PPD incidence has been hypothesized, but empirical evidence is lacking. OBJECTIVE To determine whether accelerated increases in pCRH throughout pregnancy are associated with PPD symptoms. DESIGN Pregnant women were recruited into this longitudinal cohort study. Blood samples were obtained at 15, 19, 25, 31, and 37 weeks' gestational age (GA) for assessment of pCRH, cortisol, and adrenocorticotropic hormone (ACTH). Depressive symptoms were assessed with a standardized questionnaire at the last 4 pregnancy visits and post partum. SETTING Subjects were recruited from 2 southern California medical centers, and visits were conducted in research laboratories. PARTICIPANTS One hundred adult women with a singleton pregnancy. Main Outcome Measure Symptoms of PPD were assessed at a mean (SD) of 8.7 (2.94) weeks after delivery with the Edinburgh Postnatal Depression Scale. RESULTS Sixteen women developed PPD symptoms. At 25 weeks' GA, pCRH was a strong predictor of PPD symptoms (R(2) = 0.21; beta = 0.46 [P < . 001]), an effect that remained significant after controlling for prenatal depressive symptoms. No significant associations were found for cortisol and ACTH. Receiver operating characteristic curve analyses revealed that pCRH at 25 weeks' GA is a possible diagnostic tool (area under the curve, 0.78 [P = .001]). Sensitivity (0.75) and specificity (0.74) at the ideal cutoff point (pCRH, 56.86 pg/mL) were moderate. Growth curve analyses indicated that the trajectories of pCRH in women with PPD symptoms are significantly accelerated from 23 to 26 weeks' GA. CONCLUSIONS At a critical period in midpregnancy, pCRH is a sensitive and specific early diagnostic test for PPD symptoms. If replicated, these results have implications for the identification and treatment of pregnant women at risk for PPD.
Relevant activities of extracts and constituents of animals used in traditional Chinese medicine for central nervous system effects associated with Alzheimer's disease

The centipede Scolopendra subspinipes mutilans L. Koch (‘Wugong’), the beetle Mylabris phalerata Pallas (‘Ban mao’) and the earthworm Pheretima aspergillum Chen (‘DiLong’) have a reputation in traditional Chinese medicine for reducing symptoms of central nervous system decline, including memory loss. A series of extracts of all three organisms was tested for acetylcholinesterase (AChE) inhibition and copper ion binding effects, the latter likely to reduce oxidative damage caused by excess copper. The beetle and centipede chloroform extracts showed the strongest AChE inhibitory effects (30.6% inhibition at 105 μg mL−1 and 32.3% inhibition at 167 μg mL−1, respectively) and, in the case of the centipede, this was traced to the unsaturated fatty acids present using bioassay‐guided fractionation. Cantharidin from the beetle was shown to have AChE activity (31% inhibition at 1 μM, 0.196μg mL−1), making it a major contributor to the activity of the beetle extract. The earthworm showed no AChE inhibitory activity. Since unsaturated fatty acids have not been previously reported to have AChE inhibitory activity, a series of related compounds was tested to determine structure‐activity relationships. It was found that activity existed where there was a chain length of more than 16 C atoms with at least one unsaturated bond in the chain. The carboxylic acid group was also necessary for activity. The fatty acids present in the centipede also showed the ability to bind copper ions when tested using a novel thin layer chromatography method designed to detect copper‐binding compounds. The activities reported give some support to the use of the beetle and centipede in traditional Chinese medicine for improving cognitive function.
Calpain inhibitors block Ca2+‐induced suppression of neurite outgrowth in isolated hippocampal pyramidal neurons

Ca2+ is an important regulator of neurite elongation and growth cone movements but the mechanism(s) mediating these Ca2+‐dependent effects is nuclear. Since cytoskeletal proteins are rapidly degraded by Ca2+‐dependent proteinases (calpains) in vitro and in vivo, we investigated whether Ca2+‐induced pruning or regression of neuronal processes is mediated by calpains. Isolated hippocampal pyramidal‐like neurons were cultured and the ability of the membrane‐permeable calpain inhibitors ethyl(+)‐(2S,3S)‐3‐[(S)‐methyl‐1‐(3‐methylbutylcarbamoyl)‐butylcarbamoyl]‐2‐ oxiranecarboxylate (EST) and carbobenzoxyl‐valyl‐phenylalanyl‐H (MDL 28170) to block the Ca2+ ionophore A23187‐induced suppression in neurite outgrowth was investigated. Addition of 100 nM A23187 to the culture medium resulted in a retraction of dendrites without altering axonal elongation. The addition of 300 nM A23187 to the culture medium resulted in a significant decrease in the rate of axonal elongation as well as a retraction of dendritic processes. Administration of EST (5 or 20 m̈M) to the culture medium completely blocked the pruning effect of 100 nM A23187 on dendrites and of 300 nM A23187 on axons, while EST alone did not significantly affect neurite outgrowth rate. MDL 28170 (20 m̈M) showed the same effect as EST in preventing ionophore‐induced pruning of dendrites and axons at 100 and 300 nM concentrations, respectively, of A23187. EST (20 m̈M) did not block the A23187‐induced rise of [Ca2+]i as measured with fura‐2. These results suggest that calpains play a role in Ca2+‐induced pruning of neurites in isolated hippocampal pyramidal neurons. © 1994 Wiley‐Liss, Inc.
Disinfection efficiency of chlorine dioxide gas in student cafeterias in Taiwan

In Taiwan, the food and drink requirements of students and faculty members are met by student cafeterias. The air quality within these cafeterias should satisfy the guidelines laid down by the Taiwan Environmental Protection Agency (Taiwan EPA). Accordingly, this study performed an experimental investigation into the efficiency of two different gaseous chlorine dioxide (ClO2) treatments in disinfecting a local student cafeteria, namely a single, one-off application and a twice-daily application. In both cases, the ClO2 was applied using strategically placed aerosol devices. The air quality before and after disinfection was evaluated by measuring the bioaerosol levels of bacteria and fungi. Moreover, a stepwise discriminant analysis method was applied for predicting the residual concentrations of bacteria and fungi, as a function of the environmental parameters and the ClO2 concentration. The experimental results showed that the average background levels of bacteria and fungi prior to ClO2 disinfection were 972.5 ± 623.6 and 1534.1 ± 631.8 colony-forming units (CFU)/m3, respectively. A single ClO2 application was found to reduce the bacterial and fungal concentration levels by as much as 65% and 30%, respectively. By contrast, a twice-daily ClO2 application was found to reduce the bacterial and fungal concentration levels by as much as 74% and 38%, respectively. The statistical analysis results showed that the residual bacterial concentration level was determined primarily by the number of individuals present in the cafeteria, the temperature, and the ClO2 concentration, whereas the residual fungal concentration level was determined mainly by the temperature, the total number of suspended particles, and the ClO2 concentration. Thus, the integrated results suggest that the air quality guidelines prescribed by the Taiwan EPA for student cafeteria can best be achieved by applying ClO2 twice daily using an appropriate deployment of aerosol devices. Implications: ClO2 gas can destroy all manner of microorganisms, including bacteria, spores, fungi, viruses, and even protozoans, in indoor environments. Moreover, it is popularly known that bioaerosols are able to grow and propagate on a wide variety of building materials and indoor surfaces. Thus, through optimal ClO2 disinfection methodology, the indoor microbial contaminants can be decreased and the residual concentrations of bacteria and fungi as a function of the environmental parameters and the ClO2 concentration can be predicted via some statistical techniques.
Hitler and Stalin in Perspective: Secret Speeches on the Eve of Barbarossa

Two complementary speeches made by Hitler and Stalin in the months before the invasion are uniquely revealing. On 30 March 1941 Hitler addressed the leaders of the Wehrmacht’s forces deployed in the east about the military situation and the nature of the planned campaign. Five weeks later, on 5 May, Stalin presented a similar overview of the military situation at a Red Army graduation ceremony attended by many Soviet leaders. Despite their great importance neither speech exists as a certain text, and the present article contains different sets of notes for both speeches. The notes on Hitler’s speech made by Col. Gen. Franz Halder have been available in published form for many years, but they can now be compared with a second and longer version, by Col. Gen. Hermann Hoth, which has never been published before. The first extensive notes of Stalin’s speech were published only in the 1990s, and these have more recently been supplemented by notes from the diaries of Georgi Dimitrov and V.A. Malyshev. Stalin’s speech has not, to the best of the authors’ knowledge, been published in full in English before, and neither have the Dimitrov and Malyshev notes.
Negative Feedback Control of Jasmonate Signaling by an Alternative Splice Variant of JAZ101[C][W][OA]

A transcriptional regulator generated by alternative splicing uses a cryptic binding site to negatively regulate bHLH-type transcription factors that promote jasmonate responses. The plant hormone jasmonate (JA) activates gene expression by promoting ubiquitin-dependent degradation of jasmonate ZIM domain (JAZ) transcriptional repressor proteins. A key feature of all JAZ proteins is the highly conserved Jas motif, which mediates both JAZ degradation and JAZ binding to the transcription factor MYC2. Rapid expression of JAZ genes in response to JA is thought to attenuate JA responses, but little is known about the mechanisms by which newly synthesized JAZ proteins exert repression in the presence of the hormone. Here, we show in Arabidopsis (Arabidopsis thaliana) that desensitization to JA is mediated by an alternative splice variant (JAZ10.4) of JAZ10 that lacks the Jas motif. Unbiased protein-protein interaction screens identified three related basic helix-loop-helix transcription factors (MYC2, MYC3, and MYC4) and the corepressor NINJA as JAZ10.4-binding partners. We show that the amino-terminal region of JAZ10.4 contains a cryptic MYC2-binding site that resembles the Jas motif and that the ZIM motif of JAZ10.4 functions as a transferable repressor domain whose activity is associated with the recruitment of NINJA. Functional studies showed that the expression of JAZ10.4 from the native JAZ10 promoter complemented the JA-hypersensitive phenotype of a jaz10 mutant. Moreover, treatment of these complemented lines with JA resulted in the rapid accumulation of JAZ10.4 protein. Our results provide an explanation for how the unique domain architecture of JAZ10.4 links transcription factors to a corepressor complex and suggest how JA-induced transcription and alternative splicing of JAZ10 premessenger RNA creates a regulatory circuit to attenuate JA responses.
[Severe acute respiratory syndrome: update].

INTRODUCTION The severe acute respiratory syndrome (SARS) has recently been recognised as a new clinical entity and a new emerging infectious disease that is highly contagious with significant mortality. CURRENT KNOWLEDGE AND KEY POINTS By the end of 17 June 2003, a total of 8500 cases included 800 deaths had been reported from 30 countries. The cause of SARS has been identified as a new corona virus. In this manuscript, we describe the clinical features, biological findings, virological tests, radiological assessment, outcome and treatments of this infection. FUTURE Research on specific therapies and vaccines is on-going.