Miracle-dz/UniFAR-FTUs huggingface dataset:
Interactive comment on “ Constraining remote oxidation capacity with ATom observations ” by

The article “Constraining remote oxidation capacity with ATom observations” by Travis et al., submitted for publication in Atmospheric Chemistry and Physics, presents an evaluation of the hydroxyl radical (OH) and OH reactivity (OHR) measured during the Atmospheric Tomography Mission (ATom) against the GEOS-Chem global chemical transport model. While OH is generally well-modeled in the remote regions sampled by ATom, notable biases occur in wintertime Northern Hemisphere NOy and in OHR in the lowest 3 km. Multiple explanations for these discrepancies are discussed through literature review and explored through model sensitivity simulations.
Localization of neutral evolution: selection for mutational robustness and the maximal entropy random walk

If many mutations confer no immediate selective advantage, they can pave the way for the discovery of fitter phenotypes and their subsequent positive selection. Understanding the reach of neutral evolution is therefore a key problem linking diversity, robustness and evolvability at the molecular scale. While this process is usually described as a random walk in sequence space with clock-like regularity, new effects can arise in large microbial or viral populations where new mutants arise before old ones can fix. Here I show that the clonal interference of neutral variants shuts off the access to neutral ridges and thus induces localization within the robust cores of neutral networks. As a result, larger populations can be less effective at exploring sequence space than smaller ones—a counterintuitive limitation to evolvability which invalidates analogies between evolution and percolation. I illustrate these effects by revisiting Maynard Smith’s word-game model of protein evolution. Interestingly, the phenomenon of neutral interference connects evolutionary dynamics to a Markov process known in network science as the maximal-entropy random walk; its special properties imply that, when many neutral variants interfere in a population, evolution chooses mutational paths—not individual mutations—uniformly at random.
Biomass as a sustainable resource for value‐added modern materials: a review

Over the past century, the world's population has increased greatly. The growth in the population has increased the global food demand, so the food sector has increased its production. As a result of the increased production and consumption, a huge amount of agro‐food waste is being generated yearly. Rice husk, wheat straw, sugarcane bagasse, corn husk, peanut shell, eggshell, are just a few examples of agro‐food wastes. As these agro‐food wastes and their by‐products are left unused and unattended, their amount is increasing globally. It is imperative to find ways to put these wastes to environmentally viable use. These wastes find direct uses in biofertilizers, mud houses, animal feed, biofuel, and heat generation in small‐scale industries, etc. After heat generation, ashes are considered as a secondary by‐product, which can be used as a sustainable and renewable resource for synthesizing value‐added products for various applications. This article presents a review of the traditional and advanced methods of utilizing these wastes in household applications, animal feed, fertilizers, fuel generation, pyrolysis, and for the removal of toxic metals from polluted water. These secondary resources can also be used in construction materials and for the synthesis of glasses and glass–ceramics, which can be used in bioengineering, optical, and dielectric materials. Agro‐food wastes can be used as resource materials to develop modern materials with better properties instead of using minerals. This not only reduces the environment‐ and management‐related problem of the wastes but also provides a sustainable alternative way to produce cost‐effective value‐added materials for the benefit of society. © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd
How Teachers and Principals Enact the Policy of Building Education in Sweden on a Scientific Foundation and Proven Experience: Challenges and Opportunities

ABSTRACT In 2010, Sweden was the first country in the world to introduce a legal requirement that education should be research-based, placing huge demands on schools. The study’s aim is to explore how, through sensemaking, teachers and principals enact this policy in schools. In total, 272 teachers and 23 principals from pre-schools, leisure-time centers, compulsory schools, and upper secondary schools completed a questionnaire. The findings show the need for understanding central policy concepts, alignment with previous experiences, and a social context within which the policy can be understood, negotiated, and enacted. Policy enactment was dependent on the support given – professional development, time, and financial resources. Teachers and principals have so far experienced challenges and opportunities – a rewarding if complex process.
Endoscopic mucosal resection with an additional working channel (EMR+) in a porcine ex vivo model: a novel technique to improve en bloc resection rate of snare polypectomy

Abstract Background and study aims Recently, a new external additional working channel (AWC) was introduced by which conventional endoscopic mucosal resection (EMR) can be improved to a technique termed “EMR+”. We first evaluated this novel technique in comparison to classical EMR in flat lesions. Methods The trial was prospectively conducted in an ex vivo animal model with porcine stomachs placed into the EASIE-R simulator. Prior to intervention, standardized lesions were set by coagulation dots, measuring 1, 2, 3 or 4 cm. Results Overall, 152 procedures were performed. EMR and EMR+ were both very reliable in 1-cm lesions, each showing en bloc resection rates of 100 %. EMR+ en bloc resection rate was significantly higher in 2-cm lesions (95.44 % vs. 54.55 %, P = 0.02), in 3-cm lesions (86.36 % vs. 18.18 %, P < 0.01) and also in 4-cm lesions (60.00 % vs. 0 %, P < 0.01). Perforations occurred only in EMR+ procedures in 4-cm lesions (3 of 20; 15 %). Conclusions With its grasp-and-snare technique, EMR+ facilitates en bloc resection of larger lesions compared to conventional EMR. In lesions 2 cm and larger, EMR+ has demonstrated advantages, especially concerning en bloc resection rate. At 3 cm, EMR+ reaches its best discriminatory power whereas EMR+ has inherent limits at 4 cm and in lesions of that size, other techniques such as ESD or surgery should be considered.
FTO Variant rs1421085 Associates With Increased Body Weight, Soft Lean Mass, and Total Body Water Through Interaction With Ghrelin and Apolipoproteins in Arab Population

Association studies have implicated single nucleotide polymorphisms (SNPs), particularly rs1421085, from the fat mass and obesity-associated (FTO) gene with body composition phenotypes, obesity, dietary intake, and physical activity in European, East Asian, and African populations. However, the impact of the rs1421085 variant has not been sufficiently tested in ethnic populations (such as Arabs) with high levels of obesity. Further, there is a lack of studies identifying biomarkers that interact with FTO. Therefore, we investigated the association of rs1421085 with obesity and body composition traits and metabolic biomarkers in Arab population. We genotyped rs1421085 SNP in 278 Arab individuals, where multiple biomarkers relating to obesity, inflammation, and other metabolic pathways were quantified. We performed genetic association tests under additive mode of inheritance using linear regression models and found association of rs1421085_C allele with higher levels of body weight, soft lean mass (SLM), and total body water. Examination (using linear regression models under dominant mode of inheritance) of correlation among biomarkers and interaction with genotypes at the variant revealed that measures of these three body composition traits were found mediated by interaction between carrier genotypes (TC+CC) and measures of ghrelin, ApoA1, and ApoB48. Lean body mass (LBM), to which SLM contributes, is an important determinant of physical strength and is a focal point in studies on sarcopenia. Low LBM is known to be associated with higher risk of cardiometabolic disorders. Thus, the finding on the FTO variant as a genetic determinant of SLM via interaction with ghrelin, ApoA1, and ApoB48 is important.
Commercial Formulates of Trichoderma Induce Systemic Plant Resistance to Meloidogyne incognita in Tomato and the Effect Is Additive to That of the Mi-1.2 Resistance Gene

Meloidogyne is the most damaging plant parasitic nematode genus affecting vegetable crops worldwide. The induction of plant defense mechanisms against Meloidogyne in tomato by some Trichoderma spp. strains has been proven in pot experiments, but there is no information for tomato bearing the Mi-1.2 resistance gene or for other important fruiting vegetable crops. Moreover, Trichoderma is mostly applied for managing fungal plant pathogens, but there is little information on its effect on nematode-antagonistic fungi naturally occurring in soils. Thus, several experiments were conducted to determine (i) the ability of two commercial formulates of Trichoderma asperellum (T34) and Trichoderma harzianum (T22) to induce systemic resistance in tomato and cucumber against an avirulent Meloidogyne incognita population in split-root experiments; (ii) the effect of combining T34 with tomato carrying the Mi-1.2 resistance gene to an avirulent M. incognita population in sterilized soil; and (iii) the effect of combining T34 with tomato carrying the Mi-1.2 resistance gene to a virulent M. incognita population in two suppressive soils in which Pochonia chlamydosporia is naturally present, and the effect of T34 on the level of P. chlamydosporia egg parasitism. Both Trichoderma formulates induced resistance to M. incognita in tomato but not in cucumber. In tomato, the number of egg masses and eggs per plant were reduced by 71 and 54% by T34, respectively. T22 reduced 48% of the number of eggs per plant but not the number of egg masses. T34 reduced the number of eggs per plant of the virulent M. incognita population in both resistant and susceptible tomato cultivars irrespective of the suppressive soil, and its effect was additive with the Mi-1.2 resistance gene. The percentage of fungal egg parasitism by P. chlamydosporia was not affected by the isolate T34 of T. asperellum.
Intrauterine Fetal Surgery Current Approaches

Though prenatal diagnosis has advanced a lot along with surgical techniques, intrauterine fetal surgery often not the preferred option. This is because the risks involved for both the mother and the fetus during and after the operation are often much more than the benefit to be achieved. Moreover, the infrastructure require ments and the costs of such operations are quite high. Fetal intervention is only possible in circumstances where the operation will correct abnormalities of the fetus which hamper normal growth, thus enabling normal fetal development (Twin-to-twin transfusion syndrome, congenital diaphragmatic hernia, Space-occupying thoracic lesions, Obstruction of lower urinary tract ect). It is mainly indicated in diseases that can result in intrauterine fetal death or whose effects could not be corrected postnatally (congenital aortic valve stenosis, hypoplastic left heart syndrome ect.). The proven pathophysiology and proper identification of the defect or disease is thus necessary before attempting such intervention. Since intrauterine fetal surgery entail high risks for both the fetus and the mother, there are ethical controversies regarding research in fetal surgery. Suitable surgical techniques, appropriate and effective high end methods to monitor fetal and uterine development and prevention of uterine contractions after surgery (tocolysis) are a few of the key bottlenecks that need to be addressed urgently to make intrauterine fetal surgery more useful. This article reviews recent intrauterine fetal interventions and their risk and success rates.
On-Label or Off-Label? Overcoming Regulatory and Financial Barriers to Bring Repurposed Medicines to Cancer Patients

Repurposing of medicines has gained a lot of interest from the research community in recent years as it could offer safe, timely, and affordable new treatment options for cancer patients with high unmet needs. Increasingly, questions arise on how new uses will be translated into clinical practice, especially in case of marketed medicinal products that are out of basic patent or regulatory protection. The aim of this study was to portray the regulatory framework relevant for making repurposed medicines available to cancer patients in Europe and propose specific policy recommendations to address the current regulatory and financial barriers. We outlined two routes relevant to the clinical adoption of a repurposed medicine. First, a new indication can be approved, and thus brought on-label, via the marketing authorization procedures established in European and national legislation. Such procedures initiate a detailed and independent assessment of the quality and the benefit-risk balance of a medicinal product in a specific indication, benefiting both prescribers and patients as it reassures them that the scientific evidence is robust. However, the process of marketing authorization for new therapeutic indications entails a high administrative burden and significant costs while the return-on-investment for the pharmaceutical industry is expected to be low or absent for medicines that are out of basic patent and regulatory protection. Moreover, most of the repurposing research is conducted by independent or academic researchers who do not have the expertise or resources to get involved in regulatory procedures. A second option is to prescribe a medicine off-label for the new indication, which is managed at the national level in Europe. While off-label use could provide timely access to treatments for patients with urgent medical needs, it also entails important safety, liability and financial risks for patients, physicians, and society at large. In view of that, we recommend finding solutions to facilitate bringing new uses on-label, for example by developing a collaborative framework between not-for-profit and academic organizations, pharmaceutical industry, health technology assessment bodies, payers, and regulators.
A bioinspired Separated Flow wing provides turbulence resilience and aerodynamic efficiency for miniature drones

A bioinspired wing generates lift that is robust to gusts and freestream turbulence without sacrificing flight duration. Small-scale drones have enough sensing and computing power to find use across a growing number of applications. However, flying in the low–Reynolds number regime remains challenging. High sensitivity to atmospheric turbulence compromises vehicle stability and control, and low aerodynamic efficiency limits flight duration. Conventional wing designs have thus far failed to address these two deficiencies simultaneously. Here, we draw inspiration from nature’s small flyers to design a wing with lift generation robust to gusts and freestream turbulence without sacrificing aerodynamic efficiency. This performance is achieved by forcing flow separation at the airfoil leading edge. Water and wind tunnel measurements are used to demonstrate the working principle and aerodynamic performance of the wing, showing a substantial reduction in the sensitivity of lift force production to freestream turbulence, as compared with the performance of an Eppler E423 low–Reynolds number wing. The minimum cruise power of a custom-built 104-gram fixed-wing drone equipped with the Separated Flow wing was measured in the wind tunnel indicating an upper limit for the flight time of 170 minutes, which is about four times higher than comparable existing fixed-wing drones. In addition, we present scaling guidelines and outline future design and manufacturing challenges.
Alteration of Exon Definition Causes Amelogenesis Imperfecta

Amelogenesis imperfecta (AI) is a collection of genetic disorders affecting the quality and/or quantity of tooth enamel. More than 20 genes are, so far, known to be responsible for this condition. In this study, we recruited 3 Turkish families with hypomaturation AI. Whole-exome sequence analyses identified disease-causing mutations in each proband, and these mutations cosegregated with the AI phenotype in all recruited members of each family. The AI-causing mutations in family 1 were a novel AMELX mutation [NM_182680. 1:c.143T>C, p.(Leu48Ser)] in the proband and a novel homozygous MMP20 mutation [NM_004771. 3:c.616G>A, p.(Asp206Asn)] in the mother of the proband. Previously reported compound heterozygous MMP20 mutations [NM_004771. 3:c.103A>C, p.(Arg35=) and c.389C>T, p.(Thr130Ile)] caused the AI in family 2 and family 3. Minigene splicing analyses revealed that the AMELX missense mutation increased exonic definition of exon 4 and the MMP20 synonymous mutation decreased exonic definition of exon 1. These mutations would trigger an alteration of exon usage during RNA splicing, causing the enamel malformations. These results broaden our understanding of molecular genetic pathology of tooth enamel formation.
Quantifying the depth of anesthesia based on brain activity signal modeling

Abstract Various methods of assessing the depth of anesthesia (DoA) and reducing intraoperative awareness during general anesthesia have been extensively studied in anesthesiology. However, most of the DoA monitors do not include brain activity signal modeling. Here, we propose a new algorithm termed the cortical activity index (CAI) based on the brain activity signals. In this study, we enrolled 32 patients who underwent laparoscopic cholecystectomy. Raw electroencephalography (EEG) signals were acquired at a sampling rate of 128 Hz using BIS-VISTATM with standard bispectral index (BIS) sensors. All data were stored on a computer for further analysis. The similarities and difference among spectral entropy, the BIS, and CAI were analyzed. Pearson correlation coefficient between the BIS and CAI was 0.825. The result of fitting the semiparametric regression models is the method CAI estimate (−0.00995; P = .0341). It is the estimated difference in the mean of the dependent variable between method BIS and CAI. The CAI algorithm, a simple and intuitive algorithm based on brain activity signal modeling, suggests an intrinsic relationship between the DoA and the EEG waveform. We suggest that the CAI algorithm might be used to quantify the DoA.
A region growing and local adaptive thresholding-based optic disc detection

Automatic optic disc (OD) localization and segmentation is not a simple process as the OD appearance and size may significantly vary from person to person. This paper presents a novel approach for OD localization and segmentation which is fast as well as robust. In the proposed method, the image is first enhanced by de-hazing and then cropped around the OD region. The cropped image is converted to HSV domain and then V channel is used for OD detection. The vessels are extracted from the Green channel in the cropped region by multi-scale line detector and then removed by the Laplace Transform. Local adaptive thresholding and region growing are applied for binarization. Furthermore, two region properties, eccentricity, and area are then used to detect the true OD region. Finally, ellipse fitting is used to fill the region. Several datasets are used for testing the proposed method. Test results show that the accuracy and sensitivity of the proposed method are much higher than the existing state-of-the-art methods.
Removing a single neuron in a vertebrate brain forever abolishes an essential behavior

Significance The Mauthner cell is the largest neuron known in the vertebrate brain and, in fish, mediates rapid escape behavior. Ablating this neuron has repeatedly failed to eliminate rapid escapes, a survival role of these escapes has not been supported experimentally, and it is unknown which advantage the enormous size and complexity of the cell conveys. By taking care to ensure ablations remove not only the soma but also the giant axon, we find that rapid escapes are lost forever and that this loss directly affects survival in predator–prey assays. The Mauthner cell thus is an example in which a survival-critical function depends on an individual neuron whose axon appears to have unusual capacities to remain functional after severe injury. The giant Mauthner (M) cell is the largest neuron known in the vertebrate brain. It has enabled major breakthroughs in neuroscience but its ultimate function remains surprisingly unclear: An actual survival value of M cell-mediated escapes has never been supported experimentally and ablating the cell repeatedly failed to eliminate all rapid escapes, suggesting that escapes can equally well be driven by smaller neurons. Here we applied techniques to simultaneously measure escape performance and the state of the giant M axon over an extended period following ablation of its soma. We discovered that the axon survives remarkably long and remains still fully capable of driving rapid escape behavior. By unilaterally removing one of the two M axons and comparing escapes in the same individual that could or could not recruit an M axon, we show that the giant M axon is essential for rapid escapes and that its loss means that rapid escapes are also lost forever. This allowed us to directly test the survival value of the M cell-mediated escapes and to show that the absence of this giant neuron directly affects survival in encounters with a natural predator. These findings not only offer a surprising solution to an old puzzle but demonstrate that even complex brains can trust vital functions to individual neurons. Our findings suggest that mechanisms must have evolved in parallel with the unique significance of these neurons to keep their axons alive and connected.
Higher intakes of flavonoids are associated with lower salivary IL‐1β and maintenance of periodontal health 3–4 years after scaling and root planing

Abstract Aims Higher intakes of fruits and vegetables, and vitamin C are associated with improved periodontal healing post‐scaling and root planing (SRP). This study determined if this association was sustained at 3–4 years post‐SRP, and if flavonoid intake is associated with periodontal health. Whether reduced probing depth (PD) is sustained and whether PD is correlated with salivary IL‐1β, IL‐6 and CRP at 3–4 years post‐SRP were also studied. Materials and Methods Clinical periodontal outcomes, dietary intakes and salivary markers of inflammation were measured in patients (n = 43, 23 females, 37–93 years) who had undergone SRP 3–4 years earlier and had been part of a periodontal maintenance programme. Results Flavonoid intake was inversely associated with PD (p = .042) and salivary IL‐1β concentration (p = .015) after adjustment for multiple confounders. When changes in PD were considered, the association of flavonoid intake with reduced PD became borderline significant (p = .051) but persisted for IL‐1β (p = .018). PD at 3–4 years and 2–4 months post‐SRP was similar. There was a positive correlation between PD and salivary IL‐1β (p = .005) but not with salivary CRP and IL‐6. Conclusion Higher flavonoid intake is associated with lower IL‐1β. Also, regular supportive periodontal therapy maintained the improved PD at 3–4 years post‐SRP regardless of smoking status.