Miracle-dz/UniFAR-FTUs huggingface dataset:
Monitoring Receptor Oligomerization Using Time-resolved Fluorescence Resonance Energy Transfer and Bioluminescence Resonance Energy Transfer

Oligomerization of the human δ-opioid receptor and its regulation by ligand occupancy were explored following expression in HEK293 cells using each of co-immunoprecipitation of differentially epitope-tagged forms of the receptor, bioluminescence resonance energy transfer and time-resolved fluorescence resonance energy transfer. All of the approaches identified constitutively formed receptor oligomers, and the time-resolved fluorescence studies confirmed the presence of such homo-oligomers at the cell surface. Neither the agonist ligand [d-Ala2,d-Leu5]enkephalin nor the inverse agonist ligand ICI174864 were able to modulate the oligomerization status of this receptor. Interactions between co-expressed δ-opioid receptors and β2-adrenoreceptors were observed in co-immunoprecipitation studies. Such hetero-oligomers could also be detected using bioluminescence resonance energy transfer although the signal obtained was substantially smaller than for homo-oligomers of either receptor type. Signal corresponding to the δ-opioid receptor-β2-adrenoreceptor hetero-oligomer was increased in the presence of agonist for either receptor. However, substantial levels of this hetero-oligomer were not detected at the cell surface using time-resolved fluorescence resonance energy transfer. These studies demonstrate that, following transient transfection of HEK293 cells, constitutively formed oligomers of the human δ-opioid receptor can be detected by a variety of approaches. However, these are not regulated by ligand occupancy. They also indicate that time-resolved fluorescence resonance energy transfer represents a means to detect such oligomers at the cell surface in populations of intact cells.
Assessment of the Quality of Life of Mothers of Children with Cerebral Palsy (Primary Caregivers)

Objectives. To evaluate the quality of life and psychological status in mothers of children with cerebral palsy (MCCPs) and to assess their relation with regard to the degree of their child’s disability. Design. Face-to-face interviews with MCCPs have been performed. A prospective, case control study. Setting. Physical therapy and rehabilitation training and research hospital. Subjects. 46 MCCPs and the mothers of 46 healthy children. Main Outcome Measure. Notthingam Health Profile-1, Beck Depression Inventory (BDI), and Beck Anxiety Inventory (BAI) scores in MCCPs and the mothers of healthy children. Gross Motor Function Classification System (GMFCS) was used for the children with cerebral palsy. Results. A significantly worsened (P = 0.001) quality of life was observed in MCCPs compared to the mothers of healthy children. BDI scores of the MCCPs were significantly higher than the mothers in the control group (P = 0.000). There were no significant differences in BAI scores between MCCPs and control mothers (P > 0.05). A positive correlation between the quality of life and BDI scores was noted in MCCPs (P = 0.000, r = 0.57), and no correlation was detected between the quality of life in MCCPs and the GMFCS levels of children with cerebral palsy (r = 0.012, P = 0.95). Conclusion. The quality of life in MCCPs is significantly lower than that of control mothers. Also, although the BAI scores were not significantly different between the groups, the BDI scores were poorer in MCCPs compared to those the control group.
Cranial Dural Arteriovenous Fistula: Diagnosis and Classification with Time-Resolved MR Angiography at 3T

BACKGROUND AND PURPOSE: The diagnosis of dural arteriovenous fistula (DAVF) remains one of the few uncontested indications for catheter based cerebral angiography. We report our experience of using a commercially available form of time-resolved MR angiography (trMRA) at 3T for the diagnosis and classification of a cranial DAVF compared with the reference standard of digital subtraction angiography (DSA). MATERIALS AND METHODS: A retrospective review of our patient records identified patients who had undergone trMRA at 3T and DSA for the evaluation of DAVF. The trMRA consisted of whole-head, contrast-enhanced “time-resolved imaging of contrast kinetics” (TRICKS) MRA. Image sets were independently reviewed by 3 readers for the presence, location, and classification of a DAVF. The reported result of the DSA was used as the gold standard against which the performance of the trMRA was measured. RESULTS: Forty patients were identified who had undergone DSA and trMRA for evaluation of DAVF, yielding a total of 42 cases. On DSA, the results of 7 cases were normal, 15 cases were performed for surveillance of a previously cured fistula, and a new fistula (14) or persistent (6) fistula was found in 20 cases. Of these 20 fistulas, on DSA, 13 were Borden I, 2 were Borden II, and 5 were Borden III. In 93% (39/42) of DAVF cases, the 3 readers were unanimous and correct in their independent interpretation of the trMRA, correctly identifying (or excluding) all fistulas and accurately classifying them when encountered. CONCLUSIONS: In this small series, trMRA at 3T seems be a reliable technique in the screening and surveillance of DAVF in specific clinical situations.
Analysis of the transcription regulator, CNOT7, as a candidate chromosome 8 tumor suppressor gene in colorectal cancer

Loss of heterozygosity (LOH) on the short arm of chromosome 8 occurs at high frequencies in many tumor types, including colorectal carcinoma. We have previously used microcell‐mediated chromosome transfer (MMCT) to map an approximately 7.7 Mb colorectal cancer suppressor region (CRCSR) at 8p22‐23.1. We have now taken a candidate gene approach to identify the putative tumor suppressor gene located within the CRCSR. CNOT7 encodes a subunit of the CCR4‐Not transcription complex and is located at 8p22. We showed that CNOT7 is expressed in normal colonic mucosa and in colonic crypt cells, as well as in colorectal cell lines and primary tumors. We assembled a panel of 88 primary colorectal tumors comprising 20 MSI‐high (high microsatellite instability), 19 MSI‐low and 49 MSS (microsatellite stable) tumors for mutation analysis of the CNOT7 gene. Denaturing high‐performance liquid chromatography (DHPLC) analysis of the entire coding region of the CNOT7 gene revealed only one somatic missense mutation in an MSS tumor. The rarity of somatic mutations in CNOT7, and its expression in primary colorectal tumors and cell lines, suggests that CNOT7 is not the target tumor suppressor gene in the 8p22‐23.1 CRCSR. © 2003 Wiley‐Liss, Inc.
Value relevance of earnings and book values in the Qatari Stock Exchange

Purpose The study of developed capital markets suggests that information provided in financial statements has lost its value relevance to equity holders. The purpose of this paper is to explore this issue in the emerging market of Qatar. Design/methodology/approach Following other studies in the literature, the study examines the value relevance of earnings and book values using the price valuation model provided by Ohlson (1995). A total of 215 observations were collected from all firms listed on the Qatari Stock Exchange over a period of five years (2012–2016). Findings This study suggests that the value relevance of both earnings and book values has noticeably decreased over the sample period. However, its results show that the decline in the value relevance of earnings favored book values. Research limitations/implications Like other studies, this one has limitations that suggest areas for future research. For example, in Qatar, like other emerging markets, a lack of data prevents the performance of deep analysis. Additionally, the authors only use Ohlson’s (1995) model as a framework for evaluation. It would be interesting to explore the changes when examining alternative valuation models. Another limitation is that the authors examine only two accounting measures: earnings and book values. Further research could explore changes in the value relevance of other measures, such as cash flow. Practical implications These findings provide empirical evidence regarding the value relevance of earnings and book values in an emerging market. Originality/value To the authors’ knowledge, this paper provides the first empirical evidence regarding the value relevance of earnings and book values in the emerging capital market of Qatar.
Evaluation of factors affecting CGMS calibration.

BACKGROUND The optimal number/timing of calibrations entered into the CGMS (Medtronic MiniMed, Northridge, CA) continuous glucose monitoring system have not been previously described. METHODS Fifty subjects with Type 1 diabetes mellitus (10-18 years old) were hospitalized in a clinical research center for approximately 24 h on two separate days. CGMS and OneTouch Ultra meter (LifeScan, Milpitas, CA) data were obtained. The CGMS was retrospectively recalibrated using the Ultra data varying the number and timing of calibrations. Resulting CGMS values were compared against laboratory reference values. RESULTS There was a modest improvement in accuracy with increasing number of calibrations. The median relative absolute deviation (RAD) was 14%, 15%, 13%, and 13% when using three, four, five, and seven calibration values, respectively (P < 0.001). Corresponding percentages of CGMS-reference pairs meeting the International Organisation for Standardisation criteria were 66%, 67%, 71%, and 72% (P < 0.001). Nighttime accuracy improved when daytime calibrations (pre-lunch and pre-dinner) were removed leaving only two calibrations at 9 p.m. and 6 a.m. (median difference, -2 vs. -9 mg/dL, P < 0.001; median RAD, 12% vs. 15%, P = 0.001). Accuracy was better on visits where the average absolute rate of glucose change at the times of calibration was lower. On visits with average absolute rates <0.5, 0.5 to <1.0, 1.0 to <1.5, and >or=1.5 mg/dL/min, median RAD values were 13% versus 14% versus 17% versus 19%, respectively (P = 0.05). CONCLUSIONS Although accuracy is slightly improved with more calibrations, the timing of the calibrations appears more important. Modifying the algorithm to put less weight on daytime calibrations for nighttime values and calibrating during times of relative glucose stability may have greater impact on accuracy.
Lack of impact of race on the efficacy and safety of long-acting risperidone versus placebo in patients with schizophrenia or schizoaffective disorder

This analysis aimed to assess the relationship between race and clinical response to long-acting, injectable risperidone treatment in patients with schizophrenia or schizoaffective disorder. In a 12-week, randomized, double-blind study, patients with schizophrenia or schizoaffective disorder received placebo or long-acting risperidone (25, 50 or 75 mg every 2 weeks). Data were stratified by race as identified by demographic information: Caucasian, African-American or Other. Psychotic symptoms were assessed by the Positive and Negative Syndrome Scale (PANSS); movement disorders by the Extrapyramidal Symptom Rating Scale (ESRS); adverse events (AEs) were reported spontaneously. Data were available for 439 patients: 193 Caucasian (44%), 174 (40%) African-American and 72 (16%) Other patients. Baseline characteristics were similar between racial groups, as were study completion rates (overall: 30% placebo; 48% long-acting risperidone). There was a significant effect of treatment (P<0.001), but not of race, on improvement in PANSS total scores from baseline to endpoint. ESRS scores were low throughout the study, and no significant impact of race was observed. Overall rates of AEs were similar among the racial groups. Race did not appear to impact the efficacy and tolerability of long-acting risperidone.
Clinical profile of dengue fever infection in King Abdul Aziz University Hospital Saudi Arabia.

BACKGROUND The study aimed to compare the clinical profile of all patients diagnosed with dengue viral infection at King Abdul Aziz University Hospital (KAAUH), during 2005-2008. METHODOLOGY This retrospective study included 147 patients infected with dengue virus, age < or =16 years. Laboratory and haematological data were included. RESULTS Two peaks of infection occurred during 2006 and another two in 2008. Common clinical symptoms were fever, vomiting, and abdominal pain. Common haematological abnormalities were thrombocytopenia and leucopoenia. Differences existed between the years in the percentage of patients with fever, elevated alanine aminotransferase (ALT), direct bilirubin, lactate dehydrogenase (LDH), fibrin degradation products (FDPs), and haemoglobin (Hb) levels. Differences were found in nationalities between the years, but patient nationality had no effect on disease incidence. Differences were noted in the percentages of patients' immunoglobulin M (IgM) and polymerase chain reaction (PCR) positive. There was a slight inverse correlation of IgM positive with patient age. PCR, fever, ALT, direct bilirubin, LDH, FDPs, Hb, blood transfusion, and platelet transfusion showed no correlation with age or nationality. In 2005, all the patients survived, but there were 4.55%, 25%, and 2.7% deaths during 2006-2008. CONCLUSIONS Significant differences in the clinical presentation of dengue virus (DENV) infection, indicative of a variation in disease severity from dengue fever (DF) to dengue hemhorrhagic fever (DHF)/dengue shock syndrome (DSS), were noted over the years. Possible reasons are infection with different serotypes, concurrent/sequential infection of more than one serotype, and differences in host immune responses associated with host genetic variations.
Amorphous Calcium Phosphate Composites with Improved Mechanical Properties1

Hybridized zirconium amorphous calcium phosphate (ACP)-filled methacrylate composites make good calcium and phosphate releasing materials for anti-demineralizing/remineralizing applications with low mechanical demands. The objective of this study was to assess the effect of the particle size of the filler on the mechanical properties of these composites. Photo-curable resins were formulated from ethoxylated bisphenol A dimethacrylate, triethylene glycol dimethacrylate, 2-hydroxyethyl methacrylate and methacryloxyethyl phthalate. Camphorquinone and ethyl 4-N, Ndimethylaminobenzoate were utilized as components of the photoinitiator system. After 2h of mechanical milling in isopropanol, an approximate 64% reduction in the median particle diameter was observed (27.48 μm vs. 9.98μm) for unmilled and milled wet ACP, respectively. Dry ACP showed a 43% reduction in particle size from pre- to post-milling. As well as dry composites, those that had been immersed in aqueous media were evaluated for their Young's Modulus, water sorption, biaxial tensile, three-point flexural and diametral tensile strength. Mechanical milling the filler increased the volume of fine particles in the composite specimens, resulting in a more homogeneous intra-composite distribution of ACP and a reduction in voids. Since less water diffused into the milled composites upon aqueous exposure, a marked improvement in biaxial flexure strength and a moderate improvement in flexural strength over composites with unmilled ACP was observed. This improvement in the mechanical stability of milled Zr-ACP composites may help extend their dental applicability.
Health Literacy and Preventive Health Care Use Among Medicare Enrollees in a Managed Care Organization

Background. Many older adults in Medicare managed care programs have low health literacy, and this may affect use of preventive services. Objectives. To determine whether older adults with inadequate health literacy were less likely to report receiving influenza and pneumococcal vaccinations, mammograms, and Papanicolaou smears than individuals with adequate health literacy after adjusting for other covariates. Research Design. Cross-sectional survey; home interviews with community dwelling enrollees. Subjects. Medicare managed care enrollees 65 to 79 years old in four US cities (n = 2722). Measures. Short Test of Functional Health Literacy in Adults and self-reported preventive service use. Results. In bivariate analyses, self-reported lack of preventive services was higher among individuals with inadequate health literacy than those with adequate health literacy: never had an influenza vaccination: 29% versus 19% (P = 0.000); never had a pneumococcal vaccination: 65% versus 54% (P = 0.000); no mammogram in the last 2 years: 24% versus 17% (P = 0.017); never had a Papanicolaou smear: 10% versus 5% (P = 0.002). After adjusting for demographics, years of school completed, income, number of physician visits, and health status, people with inadequate health literacy were more likely to report they had never received the influenza (OR, 1.4; 95% CI, 1.1–1.9) or pneumococcal vaccination (OR, 1.3; 95% CI, 1.1–1.7), and women were less likely to have received a mammogram (OR, 1.5; 95% CI, 1.0–2.2) or Papanicolaou smear (OR, 1.7; 95% CI, 1.0–3.1). Conclusions. Among Medicare managed care enrollees, inadequate health literacy is independently associated with lower use of preventive health services.
Mechanical properties of skinned rabbit psoas and soleus muscle fibres during lengthening: effects of phosphate and Ca2+.

1. Mechanical properties of permeabilized single fibres from rabbit psoas and soleus muscle were determined by measuring the length responses due to abrupt changes in load and the force responses due to isovelocity length changes at different phosphate and Ca2+ concentrations. 2. The length responses due to abrupt increases in load from psoas fibres showed a rapid lengthening during the change in load followed by a phase of lengthening during which the velocity gradually decreased. In soleus fibres an abrupt lengthening during the change in load was followed by a phase of lengthening during which the velocity remained constant or decreased slightly for increases in load to less than 1.45 of the isometric force (F0). For larger increases in load the velocity during this later phase first increased and thereafter decreased. 3. The initial force‐velocity curve, derived from the early part of the isotonic responses after the change in load, as well as the late force‐velocity curve derived from the force level attained during isovelocity length changes, were sensitive to phosphate. Phosphate caused a shift of the absolute force‐velocity curves of both psoas and soleus fibres towards lower values of force. In psoas fibres, the relative force‐velocity curves derived by normalization of the force level to the force developed isometrically was shifted by phosphate to smaller velocities. In soleus fibres, the initial velocity at low and intermediate relative loads (less than 1.75 F0) was increased by phosphate but at higher loads it decreased, while the late force‐velocity curve showed an overall decrease in velocity. 4. The force responses during isovelocity lengthening of psoas fibres showed an early rapid increase in force followed by a slow rise in force. The position of this break point in force was sensitive to the phosphate concentration. In soleus fibres, the force responses without phosphate showed an overshoot followed by a slow rise in force. The overshoot diminished with increasing phosphate concentration. 5. Phosphate and Ca2+ affected the force responses in psoas and soleus fibres in different ways. When the isometric starting levels were the same, force during and after the length change at submaximal activation was always less than at maximal activation in the presence of 15 mM‐phosphate. 6. The changes in the mechanical performance during lengthening caused by phosphate in psoas as well as in soleus fibres, are in agreement with a decrease in the average force per attached crossbridge. (ABSTRACT TRUNCATED AT 400 WORDS)
STRATEGI PENGEMBANGAN USAHA MIKRO KECIL MENENGAH DINAS KOPERASI DAN UKM KOTA TANGERANG SELATAN

ABSTRAK Usaha Mikro Kecil Menengah (UMKM) mempunyai fungsi yang strategis dalam perekonomian nasional, khususnya di wilayah Kota Tangerang Selatan. UMKM berperan untuk penyerapan tenaga kerja dalam pendistribusian hasil-hasil pembangunan. Jumlah UMKM yang besar dapat dimanfaatkan menjadi mitra pemerintah dalam mengelola sumber daya. Indonesia sebagai negara dengan jumlah penduduk terbesar ke 4 di dunia harus mampu mengelola sumber daya yang ada demi sebesar-besarnya untuk kemakmuran rakyat. Sumber daya yang ada tidak hanya berkaitan dengan alam tetapi juga potensi-potensi lainnya, seperti potensi pasar yang besar, potensi kemajemukan bangsa dan teknologi. Pada penelitian ini digunakan pendekatan Kualitatif. Dimana penulis menggunakan wawancara, observasi langsung ke lapangan dan analisis bahan-bahan tertulis sebagai sumber data utama. Strategi Pengembangan UMKM Kota Tangerang Selatan oleh Dinas Koperasi dan UKM Tangerang Selatan sudah cukup baik. Pengembangan UMKM yang selama ini berbentuk pelatihan, bimbingan teknis maupun workshop kewirausahaan yang diikuti oleh para pelaku UMKM di Kota Tangerang Selatan sudah memberikan efek positif bagi beberapa pelaku UMKM, walaupun belum semua UMKM mampu mengimplementasikan apa yang sudah diberikan saat pelatihanyang di adakan oleh Dinas Koperasi dan UKM Kota Tangerang Selatan. Kata Kunci: Usaha Mikro Kecil Menengah, Strategi Pengembangan
A Compact Planar MIMO Antenna System of Four Elements With Similar Radiation Characteristics and Isolation Structure

A compact planar multiple-input-multiple-output (MIMO) antenna system of four elements with similar radiation characteristics is proposed for the whole 2.4-GHz WLAN band. It consists of two proximity-coupled fed microstrip square ring patch antennas and two lambda/4 microstrip slot antennas of the same linear polarization. These two types of antennas are printed on different sides of the substrate to reduce mutual coupling. With a novel isolation structure etched on the ground plane of the FR4 substrate, high port isolation (below -25 dB) and good MIMO performance are achieved. The overall lateral size of the MIMO system is only 0.64lambda times 0.48A, and good impedance matching (S11 < 10 dB) is achieved across the operating band for all the antenna elements. Full spherical radiation patterns are measured for the MIMO system, showing similar radiation characteristics, and the gains are above 2.3 dB across the operating band.