Miracle-dz/UniFAR-FTUs huggingface dataset:
Functional responsiveness in vitro and in vivo of α/β T cell receptors expressed by the B2A2 (J11d)– subset of CD4–8– thymocytes

B2A2‐CD4‐8‐ cells represent a rare subpopulation of thymocytes normally comprising 0.5% of the total adult thymus. These cells are known to express CD3‐associated T cell receptor (TcR) α/ß molecules. In the present study we have examined the functional capacity of α/ß heterodimers on B2A2‐CD4‐8‐ cells. In the presence of monoclonal antibody (mAb) specific for either murine CD3 or TcR expressing the Vβ8‐encoded ß chain (F23.1), B2A2‐CD4‐8‐ cells proliferated. Such proliferation was blocked by mAb to interleukin 2 receptor (IL 2R), suggesting an autocrine mechanism involving IL 2 production and subsequent utilization. IL 2 and also IL 3 production by mAb‐stimulated B2A2‐CD4‐8‐ cells was directly confirmed. Furthermore, a panel of B2A2‐CD4‐8‐ clones were derived to assess the role of the TcR in cytolysis. Many clones were isolated which killed Fc receptor‐bearing P815 target cells only in the presence of F23.1 mAb. Finally, in vivo treatment of neonatal mice with F23.1 mAb resulted in a marked reduction of Vβ8 B2A2‐CD4‐8‐ thymocytes. Collectively, these results indicate that the TcR α/ß complex on CD4‐CD8‐B2A2‐ cells is fully capable of transducing signals that lead to proliferation, lymphokine production and cytolysis in vitro, as well as to disappearance of this subset from the thymus in vivo.
Adding mitochondrial sequence data (16S rRNA and cytochrome c oxidase subunit I) to the phylogeny of centipedes (Myriapoda: Chilopoda): an analysis of morphology and four molecular loci

Relationships within Chilopoda (centipedes) are assessed based on 222 morphological characters, complete 18S rRNA sequences for 70 chilopod terminals, the D3 region of 28S rRNA for 65 terminals, 16S rRNA sequences for 54 terminals and cytochrome c oxidase subunit I sequences for 45 terminals. Morphological and molecular data for seven orders of Diplopoda are used to root cladograms for Chilopoda. Analyses use direct character optimization for 15 gap and substitution models. The Pleurostigmophora and Epimorpha s.l. hypotheses are largely stable to parameter variation for the combined data; the latter clade is formalized as the new taxon Phylactometria. The combined data include parameter sets that support either the monophyly of Epimorpha s.str. (¼Scolopendromorpha + Geophilomorpha) or Craterostigmus + Geophilomorpha; the former derives its support from morphology and the nuclear ribosomal genes. Monophyly of Lithobiomorpha and the sister group relationship between Lithobiidae and Henicopidae are stable for morphological and combined data, and are also resolved for the molecular data for 14 of 15 parameter sets. The fundamental split in Scolopendromorpha is between Cryptopidae and Scolopendridae sensu Attems. Blind scolopendromorphs unite as a clade in most molecular and combined analyses, including those that minimize incongruence between data partitions. Geophilomorpha divides into Placodesmata and Adesmata under nine of 15 explored parameter sets.
Bayesian anti-sparse coding – Complementary results and supporting materials

This document contains supplementary material for [36]. Section I presents and compares two other strategies to generates samples according to the so called democratic distribution. Section II describes an additional experiments that assess the correctness of the proposed samplers, following [40]. Section III is a more detailed version of Section B in [36]. Section IV details the computation of the two first moments of the democratic distribution. Section V is dedicated to the proof of the assertion in Remark 2 of [36] concerning the convergence in law of a rescale marginal. Section VI is a detailed proof of Property 3 in [36]. Section VII briefly presents the double-sided Gamma distribution.
Phenoxodiol sensitizes metastatic colorectal cancer cells to 5-fluorouracil- and oxaliplatin-induced apoptosis through intrinsic pathway

Colorectal cancer (CRC) is one of the most common types of cancer seen in the world. 5-Fluorouracil (5-Fu) plus Oxaliplatin (1-OHP) remains the backbone of CRC chemotherapeutics, but with limited success. Phenoxodiol (Pxd) is an isoflavone analog with antitumor activity against various types of cancers, and sensitizes chemoresistant cancer cells to chemotherapeutics including platinum and taxanes. This study was, therefore, undertaken to examine whether Pxd pre-treatment with conventional chemotherapeutic agent(s) 5-Fu and 1-OHP co-administration be a therapeutic strategy for CRC. Cell viability and cytotoxicity were evaluated using dimethyl-thiazolyl diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase assays. The percentage of apoptotic and necrotic cells were determined by fluorescence microscopy analysis. Besides, active Caspase-3 levels by ELISA and relative mRNA levels of Caspase 3 (CASP3), CASP8 and CASP9 genes were determined by quantitative real-time PCR (qPCR) analysis. The pre-treatment of Pxd followed by 5-Fu and 1-OHP co-administration was more effective at inhibiting cell viability than either chemotherapeutic agents treatment alone. When compared to 5-Fu with 1-OHP alone treatment, Pxd pre-treatment overwhelmingly increased apoptotic Caspase-3 activity levels in CRC cells. Moreover, qPCR analyses showed that CASP3 and CASP9 mRNA levels significantly increased after pre-treatment with Pxd followed by 5-Fu and 1-OHP treatments, compared to 5-Fu with 1-OHP alone. Our results suggested that Pxd enhanced the in vitro antitumor activity of 5-Fu and 1-OHP. Our study also suggested that Pxd may be a potential candidate agent in advanced CRC and inclusion of Pxd to the conventional chemotherapeutic agent(s) could be an effective therapeutic strategy for CRC.
Cellular-Solid Shear Walls under Seismic Excitations

Cellular solids with deterministic periodic topologies show great potential for applications as response modification elements within structural systems, due to their ultra-light and good thermal properties. Response modification elements can provide additional stiffness and strength, resulting in reduced inter-story drifts and accelerations. A light-weight cellular solid shear wall could work efficiently for vibration mitigation in large scale structural systems. Finite Element models are developed to predict the stiffness, strength, and energy dissipation effectiveness of shear wall panels with cellular solids. The cellular structure of the shear wall essentially eliminates the potential of out of plane buckling which is prevailing in solid steelplate shear wall systems. In addition, macroscopically, the cellular structure is responsible for the observed pure shear behavior of the panel. A parametric study to quantify the mechanical properties of the cellular shear wall panels is conducted as a function of the thickness and length of the individual cell walls, and the orientation angle of the vertical cell walls. Finally, to evaluate the performance of a structure fitted with cellular shear wall system (CSWS), a model of a three-story structural frame is developed and analyzed under seismic excitation.
IMPROVEMENTS IN LIVE VM MIGRATION BY MEMORY PREDICTION

---- Live migration involves the process of moving virtual machines which is accessible to users even while migrating. Memory, storage, and network connectivity are transferred from source to destination system. The two common migration techniques used are pre-copy and post-copy migration techniques. In our system, one technique is selected based on the traffic contention of the system. The application traffic and migration traffic is calculated at source machine and destination machine and the machine having least traffic is chosen for Migration. The post-copy technique is chosen when destination has minimum traffic and pre-copy technique is chosen when source has minimum traffic. This system reduces migration time by analyzing the traffic and clustering the host into same subnet which in turn reduces downtime of the migration.
The Roles of Vision and Antennal Olfaction in Enemy Avoidance by Three Predatory Heteropterans

Abstract We examined the relative importance of vision and antennal olfaction in enemy avoidance by three heteropterans. Jumping spiders (Phidippus spp.) served as enemies of Nabicula subcoleoptrata (Kirby) and Nabis americoferus Carayon, and praying mantids (Tenodera aridifolia sinensis Saussure) were used as enemies of Sinea diadema (F.). In a greenhouse study, the number of bugs surviving enclosure with spiders or mantids was compared among individuals without vision, without antennal olfaction and controls after 0.5, 1, 4, and 24 h. For Nabicula subcoleoptrata and S. diadema, vision appeared to be of primary importance in avoiding capture by enemies. In contrast, most Nabis americoferus were killed by spiders and no significant difference in survival existed among the treatment groups. The importance of vision in predator avoidance is in contrast with a recently documented reliance on antennal olfaction for prey location in these species. Thus, stimuli from prey and predators may be segregated to maximize the efficiency of sensory information processing.
Phylogeography between valleys and mountains: the history of populations of Liolaemus koslowskyi (Squamata, Liolaemini)

The lizard genus Liolaemus is endemic to temperate South America and includes approximately 200 species. Liolaemus koslowskyi occurs in north‐western Argentina, where it is confined to a system of interior basins and valleys. This topographically complex region is now viewed as different enough that it has been suggested for recognition as a separate zoogeographical region: The Monte Desert of Mountains and Isolated Valleys. Here we use the mtDNA cytochrome b sequence data to investigate the phylogeographical pattern of L. koslowskyi and its relationships with other species of the darwinii group. Liolaemus koslowskyi is monophyletic with respect to all the species of the darwinii group included in this analysis. Three main clades were recovered within L. koslowskyi and we hypothesized that at least one of these, which shows 7% genetic divergence, is a candidate species. We discuss the phylogeographical patterns in association with the geological history of the region. The highly structured L. koslowskyi clade suggests that it has a relatively ancient history in a topographically rich, terrestrial archipelago of habitat and tectonic islands that are themselves relictual mountains and valleys.
Statistical optimization of cultural conditions for decolorization of methylene blue by mono and mixed bacterial culture techniques

Acinetobacter baumannii, Corynebacterium sp. , Cytophaga columnaris, Escherichia coli, Pseudomonas fluorescence and Pseudomonas luteola were locally isolated bacteria from sewage Disposal Lake at Jeddah, Saudi Arabia and they can decolorize methylene blue. E. coli was the most potent MB decolorizing and to a lesser extend P. luteola. Five different media were tested to elucidate medium formulation in favor of MB decolorization by E. coli and P. luteola. Ingredients of the basal medium favored the complete decolorization of 50 μg MB/ml after 84 h of fermentation. Time course decolorization of MB by E. coli indicated that 75 h of fermentation was satisfactory to decolorize 50 μg MB/ml. It was also able to decolorize different levels of MB up to 150 μg MB/ml after 95 h of fermentation. Bacterial consortium of E. coli and P. luteola was highly efficient to decolorize MB than monoculture, where the decolorization period reduced by more than 37% and increased decolorization rate (μgMB/h) up to 58%. Statistical designs of two phase multifactorial optimization (Plackett-Burman and Box-Behnken) were carried out to optimize cultural conditions to increase the efficiency of mixed culture to decolorize 150 μg MB/ml. Under the optimized conditions the decolorization period was reduced by about 31.7% and with increased decolorization rate by 46.4%. Methylene blue can be efficiently decolorized by facultative aerobic bacteria (E. coli and P. luteola). The decolorization process was markedly influenced by the composition of the fermentation medium and concentration of MB. Mixed culture of E. coli and P. luteola was highly efficient to decolorize MB than monoculture technique. The cultural conditions were considerably optimized using statistical experimental designs of PlackettBurman and Box-Behnken.
Swarm-inspired Reinforcement Learning via Collaborative Inter-agent Knowledge Distillation

Reinforcement Learning (RL) has demonstrated promising results across several sequential decision-making tasks. However, RL struggles to learn efﬁciently, thus limiting its pervasive application to several challenging problems. A typical RL agent learns solely from its own trial-and-error experiences, requiring many experiences to learn a successful policy. To alleviate this problem, we pro-pose collaborative inter-agent knowledge distillation (CIKD). CIKD is a learning framework that uses an ensemble of RL agents to execute different policies in the environment while sharing knowledge amongst agents in the ensemble. Our experiments demonstrate that CIKD improves upon state-of-the-art RL methods in sample efﬁciency and performance on several challenging MuJoCo benchmark tasks. Additionally, we present an in-depth investigation on how CIKD leads to performance improvements.
EUROPEAN ACADEMIC RESEARCH, VOL

Constructed wetlands are human induced systems that impersonate the functions of natural wetlands which are applied for sewage treatment. A study is carried out to investigate feasibility and optimization in pollution concentration through Angular Horizontal Subsurface Flow Constructed Wetland in the sewage treatment using Pennisetum purpureium Schumach. The plant species are implanted and propagates in the bed of constructed wetland. The sampling and analysis through physico-chemical and biological parameters viz. pH, EC, TSS, TDS, TS, BOD, COD, NO3, PO4 and SO4 were carried out at both inflow and outflow in the pilot plant. The sewage samples with different concentrations such as 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% were tested before and after treatment. Results revealed that pH range was changed from 6.72 to 7.22, maximum reduction of E.C was reduced by 34.61% at 80% sewage concentration, TSS was reduced by 55.17%, TDS was reduced by 56.18%, TS was reduced by 55.48%, BOD was reduced by 76.65%, COD was reduced by 77.51%, NO3 was reduced by 74.62%, PO4 was reduced by 57.81% and SO4 was reduced by 51.06% respectively provided after 96 hrs (4 days) Hydraulic Retention Time. The color and odour were removed and observed samples of treated water were very clear and odorless. The overall results indicated that, maximum pollutants removal were obtained in sewage with Pennisetum purpureium by root zone bed technology in constructed wetland at 80% to 90% sewage 1 Corresponding author: vpdhulap@gmail.com V. P. Dhulap, S. S. PatilRemoval of Pollutants from Sewage through Constructed Wetland using Pennisetum purpureium EUROPEAN ACADEMIC RESEARCH Vol. II, Issue 1 / April 2014 544 concentration. This model removes maximum pollutants and useful for reducing surface and subsurface water pollution load.
Low Power and Low Latency Line Streaming Image Processing for 3D User Interface on Smart Devices

A low-power 3D hand-gesture tracking (HGT) processor is proposed for real-time user interaction in smart devices. It is optimized for 3-level (Functional, processing, and ALU): 1) Region of hands are extracted in low resolution and it reduces overall redundant 3D HGT computation; 2) Line streaming CNN processing improves pipeline utilization and reduces overall 3D HGT latency; 3) Shifter-based stereo aggregation processing elements reduce power and area. The proposed chip facilitates real-time 3D HGI with 33.5mW and maximally 10.8mm error.
The effect on biochemical composition of different concentrations probiotic fed to juvenile carp (Cyprinus carpio, L. 1758) in a recirculating aquaculture system.

Knowledge about biochemical composition of flash fish allow estimation about: general maintenance conditions, methods of availability to natural and supplementary trophy basis, nutritive value of flash and establishing the impact of environmental conditions to metabolic processes. In this study, some biochemical determinations of carp (Cyprinus carpio, L. 1758) tissues were investigated. The biochemical analysis of the meat has been effectuated in the experiment that took place during 60 days, in four breeding units of 500 liters in volume each. Four kind of variants were compared: V1-pellets with 30% crude protein, without probiotics; V2-pellets with 30% crude protein, with probiotics of 2.24×109 CFU/kg food; V3-pellets with 30% crude protein, with probiotics of 3.84×109 CFU/kg food; and V4-pellets with 30% crude protein, with probiotics of 7.04×109 CFU/kg food. We used BioPlus® 2B probiotics (a mixture of Bacillus licheniformis (DSM 5749) and Bacillus subtilis (DSM 5750). After analyses the biochemical composition in stage one was: V1-16.89% proteins, 1.57% lipids; V2-16.47% proteins, 2.5% lipids; V3-16.72% proteins, 1.87% lipids; V4-15.52% proteins, 1.69% lipids and in the second stage was: V1-14.47% proteins, 1.63% lipids; V2-15.70% proteins, 3.12% lipids; V3-14.45% proteins, 1.85% lipids; V4-15.24% proteins, 2.35% lipids. The obtained results showed that are insignificant differences (p>0.05) in the levels of proteins, fats and ash between the control variant (V1) and experimental variants (V2, V3, V4) where probiotic supplements were used.
“Perfection” Subverted? A Contrivance for Outcrossing in a Rare Orchid Is Influenced by Pollinator Abundance1

Abstract The combination of protandrous flowers and acropetal inflorescence development in bee-pollinated species is thought to maximize cross-pollination because bees visit vertical inflorescences from the bottom up. However, incomplete protandry may allow bees to carry out geitonogamous pollinations. We examined the overlap in male and female phases in the rare orchid Spiranthes diluvialis, a plant with the above combination of characteristics. We found that unvisited male phase flowers proceed to a hermaphroditic phase, not a female phase, because each flower's single pollinarium remains viable and may be removed by pollinators throughout anthesis. Pollinator visitation rates, as estimated by pollinaria removal rates, varied among five populations in Utah and Colorado, USA. More hermaphroditic phase flowers accrued on inflorescences in populations with low visitation rates than in those with higher visitation rates. We conclude that the cross-pollination mechanism of S. diluvialis requires some minimum threshold of bee visits in order to work optimally. When bees are plentiful, male and female functions remain temporally separated and cross-pollination is maximized. In contrast, the potential for geitonogamy in this self-compatible species is much higher in populations with low visitation rates. We suggest that pollinator abundance may affect the mating system of other protandrous, acropetal, bee-pollinated plants if protandry depends upon the timely removal of long-lived pollen.
In vitro anticancer activity screening of Iridaceae plant extracts

Olha Mykhailenko1*, Roman Lesyk2,3, Nataliya Finiuk4, Rostyslav Stoika4, Tetyana Yushchenko5, Anna Ocheretniuk5, Valentina Vaschuk5, Volodymyr Mishchenko6, Victoriya Georgiyants7 1Department of Botany, National University of Pharmacy, Kharkiv, Ukraine. 2Department of Pharmaceutical, Organic and Bioorganic Chemistry, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine. 3 Department of Public Health, Dietetics and Lifestyle Disorders, Faculty of Medicine, University of Information Technology and Management in Rzeszow, Rzeszow, Poland. 4Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology of National Academy of Sciences of Ukraine, Lviv, Ukraine. 5Department of Pharmaceutical Chemistry, Vinnytsia National Pyrogov Memorial Medical University, Vinnytsia, Ukraine. 6Department of Quality, Standardisation and Certification of Drugs, National University of Pharmacy, Kharkiv, Ukraine. 7Department of Pharmaceutical Chemistry, National University of Pharmacy, Kharkiv, Ukraine.
Quantitative experimental comparison of single-beam, sidescan, and multibeam benthic habitat maps

Map comparison is a relatively uncommon practice in acoustic seabed classification to date, contrary to the field of land remote sensing, where it has been developed extensively over recent decades. The aim here is to illustrate the benefits of map comparison in the underwater realm with a case study of three maps independently describing the seabed habitats of the Te Matuku Marine Reserve (Hauraki Gulf, New Zealand). The maps are obtained from a QTC View classification of a single-beam echosounder (SBES) dataset, manual segmentation of a sidescan sonar (SSS) mosaic, and automatic classification of a backscatter dataset from a multibeam echosounder (MBES). The maps are compared using pixel-to-pixel similarity measures derived from the literature in land remote sensing. All measures agree in presenting the MBES and SSS maps as the most similar, and the SBES and SSS maps as the least similar. The results are discussed with reference to the potential of MBES backscatter as an alternative to SSS mosaic for imagery segmentation and to the potential of joint SBES – SSS survey for improved habitat mapping. Other applications of map-similarity measures in acoustic classification of the seabed are suggested.
Dance and movement therapy approaches for patients and disabled clients: theoretical, methodological and practical peculiarities

Dance and movement therapy is the object of the study in the article. The aim of the study is to determine theoretical, methodological and practical peculiarities of modern dance and movement therapy. The task of the study is to analyze theoretical, methodological and practical peculiarities of modern dance and movement therapy. Research problem is the gap between theory and practical issues in the dance and movement therapy. The definition of scientific novelty: there is not enough dance and movement therapy research work done in Lithuania (where dance and movement therapy is completely new phenomenon) and foreign countries. Research relevance is related to the lack of nonverbal upbringing of the temper and consulting services (e.g. dance and movement therapy) for the customers and patients, where verbal consulting is not efficient enough. That is why the analysis of theoretical, methodological and practical peculiarities of modern dance and movement therapy is so novel. Analysis and synthesis of scientific literature is the research method, which allows to represent research works done in theoretical, methodological and practical sections of Lithuanian and foreign authors. The analysis overtakes the use and influence of modern dance and movement therapy on individual and helps to solve his/hers physical, emotional and social problems. Results. According to the works of the Lithuanian and international authors, the article explored the use of dance and movement therapy orientated to the solution of physical, emotional and social problems of patients and clients (ill patients and disabled people) with the aim to use variety of dance styles and methods. Conclusion: it was determined, that modern dance and movement therapy, based on various modern theories of psychotherapy that holistically unite the solutions of mind and body problems claiming to become one of the psychotherapy forms, is applicable to different patients according to their needs when verbal therapy does not perform effectively or altogether with verbal psychotherapy. Most importantly it means to create promoting research into methods of dance and movement therapy and nationally validated, professionally recognized training for specialists of dance and movement therapy in Lithuania.