Miracle-dz/UniFAR-FTUs huggingface dataset:
Nutritional quality of aqueous alcohol extracted guar (Cyamopsis tetragonoloba) meals

Detoxification of guar meal was done by repeated extraction of the defatted meal with aqueous ethanol, methanol, or 2-propanol. The nutritive quality of the detoxified meals as compared to that of defatted meal was evaluated by both in vivo and in vitro techniques. The free polyphenol content of the detoxified meals was lower, while their trypsin inhibitor activity was higher than that of the defatted meal. Detoxification treatments resulted in a significant improvement in the in vitro digestibility of proteins. The amino acid composition and available lysine content of all the samples were comparable. The rat bioassays indicated that the nutritive value of the meal proteins improved significantly by the detoxification treatments. Weanling rats fed on diets containing detoxified meals gained 35-46 g of body weight in 4 wk, while those fed the defatted meal diet lost wt. The values for PER, net protein ratio and NPU of the detoxified guar meals were in the ranges 2.2-2.6, 3.8-4.4 and 55-59, resp.
Local Treatment of Hepatic Metastases with Low-Level Direct Electric Current: Experimental Results

Background: Several authors have recently reported encouraging results from low-level direct current therapy in easily accessible malignant tumors. However, antitumoral effects in colorectal metastases have not been investigated experimentally. Methods: Using an animal model with induced hepatic metastases, we analyzed the effectiveness and the tumor growth dynamics after direct current application. Three weeks after induction tumor volumes were estimated with magnetic resonance imaging (MRI). Then direct current (80 C/cm3) was applied in the treatment group by means of one anode in the tumor center and four cathodes peripherally. In the control group electrodes were placed without applying current. Tumor growth dynamics was analyzed with MRI after 3 and 5 weeks. After this all animals were killed, and the livers histologically examined. Results: After 5 weeks MRI showed a 1.6-fold tumor enlargement in the treatment group versus a 2.9-fold enlargement in the control group (Student t test, P = 0.0051). The histopathologic analysis of the treated livers yielded a 21% complete response rate and a 78% partial response rate. No necroses were found in the control group. Conclusions: These results confirm the effectiveness of low-level direct current application as a potential modality for the treatment of hepatic metastases.
Why is arabica coffee visited by so few non-Apis bees in its native range?

The current knowledge about pollinators in sub-Saharan Africa is extremely scarce. General pollinator distributions and resource usages are mainly unknown, as are the main pollinators of different crops (Timberlake and Morgan 2018). In early 2011, I travelled to an Afromontane area of Ethiopia, where arabica coffee, Coffea arabica L. , has its origin, to survey coffee pollinators. I surveyed 19 coffee sites, most managed with organic practices, across a gradient from state-owned, shaded semi-plantation coffee to coffee grown sparsely, more or less wild, in the understory of disturbed natural forests (Fig 1a, b).
Other Soft Tissue Pain Conditions

The authors studied more than 1700 in-patients with various musculoskeletal disorders or cardiovascular diseases in four orthopedic and six cardiovascular rehabilitation hospitals in southwest Germany. On the basis of a general health questionnaire score, 205 patients with musculoskeletal diseases and 164 patients with cardiovascular diseases were randomly selected for study. Of the 672 with musculoskeletal diseases, 205 were intereviewed using the Composite International Diagnostic Interview. The patients interviewed had a mean age of 49 years [range 22 to 75 years]. Six percent had soft tissue rheumatism whereas 69 percent had neck and back pain. Disgnosis of psychiatric disorders were based on the Diagnostic and Statistical Manual of Mental Disorders, 4th edition [DSM-IV]. Of patients with musculoskeletal disease, 31.1 percent had one or more psychiatric disorders during the rehabilitation process.This is incontrast to19.5percentof the patients with cardiovascular diseases. The psychiatric problems manifested by the musculoskeletal disease patients ranged from an affective disorder to substance abuse or dependence to anxiety disorder. The authors conclude that patients enrolled in a medical rehabilitation program, particularly as an in-patient, should be screened for psychiatric problems and treated appropriately.
Neoadjuvant Chemotherapy Plus Conventional Radiotherapy or Accelerated Hyperfractionation in Stage III and IV Nasopharyngeal Carcinoma - A Phase II Study

A prospective phase II trial was initiated in previously untreated patients with locally advanced nasopharyngeal carcinoma (NPC). The goal was to achieve improvement in locoregional control, disease-free interval and overall survival using induction chemotherapy and to compare conventional fractionation (CF) with an accelerated hyperfractionation (AHF) regimen. Fifty patients were treated (5 AJCC Stage III, 45 Stage IV) with induction chemotherapy consisting of two cycles of cisplatin and 5-fluorouracil. Patients were then randomized between CF and AHF therapy. A clinical response to induction chemotherapy was reported in 86% of patients prior to radiotherapy (44% complete response, 42% partial response). Patients with complete or major partial responses to induction chemotherapy had a significantly better 5-year overall survival (60%) and disease-free interval (59%) than those with no response or minor partial response (15% and 18%, p = 0.009 and 0.0009). Acute radiation reactions were more pronounced in the AHF group (p = 0.0002), and the incidence of late normal tissue injury was more frequent (p = 0.08). At 5 years, the locoregional control rate was higher in the AHF arm (76%) than in the CF group (54%), but the difference was not significant (HR, 0.52; 95% CI, 0.15? 2.83; p = 0.186). With a median follow-up period of 55 months (range 4? 120), the 5-year disease-free interval and overall survival rates were more favorable in the AHF group than in the CF group, but the differences were not significant (59% and 54% vs. 34% and 36%, respectively, HR for disease-free interval=0.71; 95% CI, 0.27? 1.88; p = 0.198 and HR for overall survival = 0.81; 95% CI, 0.37? 1.78; p = 0.433). The overall treatment failure rate was 48%. Locoregional failures occurred in 12 patients (24%) and the incidence of distant metastases reached 30%. Response to induction chemotherapy is strongly predictive for locoregional control, disease-free interval and overall survival. Accelerated hyperfractionation was associated with high incidence of acute and late toxicity without significant improvement in locoregional control rate. The optimal chemotherapy dose and sequencing with radiotherapy needs to be investigated in future studies. Distant metastases remain the main cause of treatment failure in NPC.
Mindfulness and yoga for psychological trauma: systematic review and meta-analysis

ABSTRACT Mindfulness-based interventions (MBIs), with postures, breath, relaxation, and meditation, such as Mindfulness-based Stress Reduction (MBSR) and yoga, are complex interventions increasingly used for trauma-related psychiatric conditions. Prior reviews have adopted a disorder-specific focus. However, trauma is a risk factor for most psychiatric conditions. We adopted a transdiagnostic approach to evaluate the efficacy of MBIs for the consequences of trauma, agnostic to diagnosis. AMED, CINAHL, Central, Embase, Pubmed/Medline, PsycINFO, and Scopus were searched to 30 September 2018 for controlled and uncontrolled trials of mindfulness, yoga, tai chi, and qi gong in people specifically selected for trauma exposure. Of >12,000 results, 66 studies were included in the systematic review and 24 controlled studies were meta-analyzed. There was a significant, pooled effect of MBIs (g = 0.51, 95%CI 0.31 to 0.71, p < .001). Similar effects were observed for mindfulness (g = 0.45, 0.26 to 0.64, p < .001), yoga (g = 0.46, 0.26 to 0.66, p < .001), and integrative exercise (g = 0.94, 0.37 to 1.51, p = .001), with no difference between interventions. Outcome measure or trauma type did not influence the effectiveness, but interventions of 8 weeks or more were more effective than shorter interventions (Q = 8.39, df = 2, p = .02). Mindfulness-based interventions, adjunctive to treatment-as-usual of medication and/or psychotherapy, are effective in reducing trauma-related symptoms. Yoga and mindfulness have comparable effectiveness. Many psychiatric studies do not report trauma exposure, focusing on disorder-specific outcomes, but this review suggests a transdiagnostic approach could be adopted in the treatment of trauma sequelae with MBIs. More rigorous reporting of trauma exposure and MBI treatment protocols is recommended to enhance future research.
Host syndecan-1 promotes listeriosis by inhibiting intravascular neutrophil extracellular traps

Heparan sulfate proteoglycans (HSPGs) are at the forefront of host-microbe interactions. Molecular and cell-based studies suggest that HSPG-pathogen interactions promote pathogenesis by facilitating microbial attachment and invasion of host cells. However, the specific identity of HSPGs, precise mechanisms by which HSPGs promote pathogenesis, and the in vivo relevance of HSPG-pathogen interactions remain to be determined. HSPGs also modulate host responses to tissue injury and inflammation, but functions of HSPGs other than facilitating microbial attachment and internalization are understudied in infectious disease. Here we examined the role of syndecan-1 (Sdc1), a major cell surface HSPG of epithelial cells, in mouse models of Listeria monocytogenes (Lm) infection. We show that Sdc1-/- mice are significantly less susceptible to both intragastric and intravenous Lm infection compared to wild type (Wt) mice. This phenotype is not seen in Sdc3-/- or Sdc4-/- mice, indicating that ablation of Sdc1 causes a specific gain of function that enables mice to resist listeriosis. However, Sdc1 does not support Lm attachment or invasion of host cells, indicating that Sdc1 does not promote pathogenesis as a cell surface Lm receptor. Instead, Sdc1 inhibits the clearance of Lm before the bacterium gains access to its intracellular niche. Large intravascular aggregates of neutrophils and neutrophil extracellular traps (NETs) embedded with antimicrobial compounds are formed in Sdc1-/- livers, which trap and kill Lm. Lm infection induces Sdc1 shedding from the surface of hepatocytes in Wt livers, which is directly associated with the decrease in size of intravascular aggregated NETs. Furthermore, administration of purified Sdc1 ectodomains or DNase inhibits the formation of intravascular aggregated neutrophils and NETs and significantly increases the liver bacterial burden in Sdc1-/- mice. These data indicate that Lm induces Sdc1 shedding to subvert the activity of Sdc1 ectodomains to inhibit its clearance by intravascular aggregated NETs.
A Flexible and Low-Cost Tactile Sensor Produced by Screen Printing of Carbon Black/PVA Composite on Cellulose Paper

This study presents the design and fabrication of a flexible tactile sensor printed on a cellulose paper substrate using a carbon black (CB) – filled polyvinyl alcohol (PVA) polymer matrix as ink material. In the design, electrodes are obtained by screen printing of CB/PVA composite on dielectric cellulose paper. The screen-printing method is preferred for fabrication because of its simplicity and low manufacturing cost. The tactile sensor is formed by overlapping two ink-printed sheets. Electrical properties are investigated under compressive and tensile strains. The results indicate that the tactile sensor configuration and materials can be used for piezoresistive, capacitive, and also impedance sensors. The same tactile sensor structure is also examined using a commercial carbon-based ink for performance comparison. The comparative study indicates that CB/PVA ink screen-printed on paper demonstrates superior sensitivity for capacitive sensing with low hysteresis, as well as low response and recovery times. The piezoresistive-sensing properties of CB/PVA on cellulose paper show a gauge factor (GF) of 10.68, which is also very promising when conventional metal strain gauges are considered. CB/PVA screen-printed on cellulose paper features impedance-sensing properties and is also sensitive to the measurement frequency. Therefore, the response type of the sensor can be altered with the frequency.
Discrete modulation of anti-predatory and agonistic behaviors by sensory communication signals in juvenile crayfish

ABSTRACT We investigated how the exchange of sensory signals modulates the individual behaviors of juvenile crayfish in an anti-predatory context as well as during intraspecific agonistic encounters. We first compared crayfish housed in total sensory isolation or in pairs with access to chemical and visual cues. After 1 week of housing, we analysed their individual responses to a visual danger signal while they were foraging. We found that crayfish previously housed in pairs with exchange of sensory signals responded to a simulated predator attack predominantly with freezing behavior, whereas animals deprived of all sensory communication mostly responded by performing escape tail-flips. Next, we used the same housing conditions in between repeated fights in pairs of crayfish. Aggressive and submissive behaviors increased in subsequent fights both after total isolation and after exchange of olfactory and visual signals. Thus, unlike responses to simulated predator attacks, intraspecific agonistic behavior was not modulated by exposure to the same sensory signals. However, when we tested the effects of olfactory or visual communication independently, aggression increased dramatically after the exchange of olfactory signals, which also led to a high number of rank reversals in second fights, suggesting a destabilization of the original dominance relationship. Exposure to visual cues during the 1-week separation, however, produced the opposite effect, reducing agonistic behaviors and rank reversals. These findings demonstrate that exchange of sensory signals modulates future anti-predatory decision-making and intraspecific agonistic behaviors discretely, suggesting that the effect of these signals on shared neural circuitry is context dependent. Summary: The exchange of sensory signals shapes future behavior of juvenile crayfish in response to threat signals and during intraspecific agonistic encounters.
Origins of Dissolved Organic Matter in Southern California Coastal Waters: Experiments on the Role of Zooplankton

The contribution of zooplankton to flows of dissolved organic carbon was studied for the food web of coastal plankton assemblages off Southern California, USA. Seawater samples including active macrozooplankton were labelled with I4CO2. The time course of I4CO2 incorporation into size fractions of the plankton and dissolved organic materials was determined. Changes in extracellular concentrations of free primary amines and dissolved saccharides were also measured. In addition, the time course of 3H-thymidine incorporation into bacteria was determined along with bacterial cell counts. By comparing carbon flows in samples with macrozooplankton and without macrozooplankton, we evaluated the role of macrozooplankton in the flux of carbon to bacteria and dissolved organic matter. One-half or more of theI4C-carbon in the bacterial size fraction could be due to small photosynthetic forms or the remains of microplankton disrupted by the filtration used in the size fractionation. Moreover, most of the carbon flowing to bacteria from the macrozooplankton was not labelled by I4C and was proportional to the number of macrozooplankton. Conservative estimates of this unlabelled carbon flux to bacterial biomass due to macrozooplankton were 3 and 10 % of the I4C assimilated photosynthetically. Potential artefacts resulting from experlmental manipulations are considered.
Evaluation of a Novel Boron-Containing α-d-Mannopyranoside for BNCT

Boron neutron capture therapy (BNCT) is a unique anticancer technology that has demonstrated its efficacy in numerous phase I/II clinical trials with boronophenylalanine (BPA) and sodium borocaptate (BSH) used as 10B delivery agents. However, continuous drug administration at high concentrations is needed to maintain sufficient 10B concentration within tumors. To address the issue of 10B accumulation and retention in tumor tissue, we developed MMT1242, a novel boron-containing α-d-mannopyranoside. We evaluated the uptake, intracellular distribution, and retention of MMT1242 in cultured cells and analyzed biodistribution, tumor-to-normal tissue ratio and toxicity in vivo. Fluorescence imaging using nitrobenzoxadiazole (NBD)-labeled MMT1242 and inductively coupled mass spectrometry (ICP-MS) were performed. The effectiveness of BNCT using MMT1242 was assessed in animal irradiation studies at the Kyoto University Research Reactor. MMT1242 showed a high uptake and broad intracellular distribution in vitro, longer tumor retention compared to BSH and BPA, and adequate tumor-to-normal tissue accumulation ratio and low toxicity in vivo. A neutron irradiation study with MMT1242 in a subcutaneous murine tumor model revealed a significant tumor inhibiting effect if injected 24 h before irradiation. We therefore report that 10B-MMT1242 is a candidate for further clinical BNCT studies.
Evolution characteristics and policy implications of new urbanization in provincial capital cities in Western China

New urbanization is the fundamental approach to achieve the healthy, stable, and sustainable development of the Chinese economic society. It is also the basic outlet to eliminate the “dual economic structure” in urban and rural areas. Based on the connotation of new urbanization, we constructed an evaluation system using population development, economic development, quality of life, infrastructure, resources and environment, and urban and rural harmonious development. The entropy and weighted summation methods were used to measure the level of new urbanization for 11 provincial capital cities from 2005 to 2018, and policy implications were analyzed correspondingly. The results show that there are significant differences in the development levels of new urbanization in these cities, with infrastructure construction being the primary driver. These developments have placed the economy and environment under great pressure. The quality of urban life and the level of infrastructure construction need to be improved because of the expanding economic gap between urban and rural areas. These cities with poor internal coordination also have apparent differences amongst individual factors. Overall, the policies on these factors play a positive role in the process of new urbanization. In the future, provincial capital cities need to consider the weak links and provide more focus on employment and education.
The Nation-wide Swedish Family-Cancer Database&Updated Structure and Familial Rates

The Swedish Family-Cancer Database was expanded to include all Swedes born in 1932 and later (offspring) with their parents, totaling 10.2 million individuals. Cancer cases were retrieved from the Swedish Cancer Registry from the years 1958 to 1998, including over 1 million primary cancers and in situ tumors. Some 10% of offspring diagnosed with cancer lack any parental information. Incidence rates of cancers were similar in the database and in the Cancer Registry to age 70, but at higher ages the rates in the Database were lower, probably because of selection. The familial risk for all types of cancer in offspring was 1.73 when a parent had the same type of cancer. The familial rates were increased for all main cancer sites, except for the upper aerodigestive tract, stomach, liver, pancreas and bone marrow (leukemia). The rates were 7.47 for thyroid, 4.69 for testis, and over 2.00 for melanoma, ovary, prostate, skin, endocrine glands and endometrium.
Evidence for genetic differences in the offspring of two sympatric morphs of Arctic charr

The sub-arctic Lake Fjellfrosvatn, northern Norway, has two morphs of Arctic charr that are reproductively isolated because they spawn 5 months apart. The smaller morph (≤14 cm LF) is confined to the profundal zone of the lake and the larger morph is mainly littoral. Three hypotheses were tested: (i) the offspring of the profundal Arctic charr grow slower than the offspring of the littoral Arctic charr under identical conditions, thus indicating a genetic basis for the slow growth of the profundal Arctic charr in the wild; (ii) the wild phenotypes of the two morphs are morphometrically different and the differences are persistent in the offspring; (iii) the offspring of the two morphs have different behaviour traits under similar treatments. The first hypothesis was rejected; offspring of the profundal morph grew slightly better than offspring of the littoral morph at 10° C in the laboratory. The second and third hypotheses were supported by the data. Wild-caught fish of the two morphs differed in several morphometric characters and most of the differences persisted in the offspring. In the laboratory, offspring of the littoral morph were more active, more aggressive and more pelagic than offspring of the profundal morph and naive offspring of the profundal morph were more effective in eating live chironomid larvae than were offspring of the littoral morph. The data for morphometry and behaviour, but not growth, provide evidence for genetic differences between the two Arctic charr morphs of Fjellfrosvatn.
Bioluminescence-Based Energy Transfer Using Semiconductor Quantum Dots as Acceptors

Bioluminescence resonance energy transfer (BRET) is the non-radiative transfer of energy from a bioluminescent protein donor to a fluorophore acceptor. It shares all the formalism of Förster resonance energy transfer (FRET) but differs in one key aspect: that the excited donor here is produced by biochemical means and not by an external illumination. Often the choice of BRET source is the bioluminescent protein Renilla luciferase, which catalyzes the oxidation of a substrate, typically coelenterazine, producing an oxidized product in its electronic excited state that, in turn, couples with a proximal fluorophore resulting in a fluorescence emission from the acceptor. The acceptors pertinent to this discussion are semiconductor quantum dots (QDs), which offer some unrivalled photophysical properties. Amongst other advantages, the QD’s large Stokes shift is particularly advantageous as it allows easy and accurate deconstruction of acceptor signal, which is difficult to attain using organic dyes or fluorescent proteins. QD-BRET systems are gaining popularity in non-invasive bioimaging and as probes for biosensing as they don’t require external optical illumination, which dramatically improves the signal-to-noise ratio by avoiding background auto-fluorescence. Despite the additional advantages such systems offer, there are challenges lying ahead that need to be addressed before they are utilized for translational types of research.
Diagnostic value of advanced semen analysis in evaluation of male infertility

Conventional semen analysis is the standard of care to initially evaluate the fertility status of a male patient. However, it has some limitations and among these are failure to correctly identify the aetiology underlying fertility problems, intra‐ and inter‐observer variability and incomplete information about sperm function. Considering these drawbacks, advanced semen tests have been developed to assess male infertility, including sperm function tests, oxidative stress (OS) and sperm DNA fragmentation (SDF) tests. This review illustrates the commonly utilised sperm function techniques, along with the assays used to assess SDF and OS and their diagnostic value.