Miracle-dz/UniFAR-FTUs huggingface dataset:
Synergistic Cytotoxicity against Human Tumor Cell Lines by Oncolytic Adenovirus dl1520 (ONYX-015) and Melphalan

Aims and background In light of the need for more selective anticancer therapy, much work has been directed at developing compounds or biological agents that target functions specific to cancer cells. To this end, numerous viruses have been engineered to exploit the dependence of cancer cells on particular anomalies that contribute to their rogue proliferative activity, such as dysfunctional p53, overactive mitogenic signaling, or a defective interferon response. The oncolytic human adenovirus dl1520 (ONYX-015) was engineered to propagate specifically in p53-deficient tumors, which comprise over half of all tumors. Based on successes in clinical trials, the full potential of dl1520 and other oncolytic viruses may be even better realized by using them in combination with conventional chemotherapy drugs. Methods As a model system in which to test this potential, representative cell lines from 2 common cancer types, oral squamous cell carcinoma (HN-5a) and colon adenocarcinoma (HT-29), were chosen, as well as platinum-drug-resistant variants of each. Results Following preliminary screening of virus and drug combinations, dl1520 and melphalan were found to synergistically inhibit proliferation of all the cancer cell lines. Melphalan pretreatment or cotreatment with dl1520 enhanced inhibition of proliferation by dl1520 by up to 60% and increased apoptosis by up to 25%. The tight-junction protein CAR (coxsackie and adenovirus receptor), via which adenovirus enters cells, was not upregulated by treatment with melphalan, suggesting that other mechanisms contribute to synergy. Conclusions The synergy between melphalan and dl1520 suggests that tumor-selective cell killing by oncolytic viruses may be augmented by combining with cytotoxic drugs.
Renal involvement in leptospirosis--our experience in Madras City.

Leptospirosis is an infectious disease caused by leptospira interrogans complex which has over 20 sero groups and more than 200 serovars. The major vectors to humans are rodents, though other animals such as cattle, dogs and pigs can also transmit the illness. These organisms are excreted in the urine of the animals and they affect man when he comes into contact with the urine of infected animals directly or indirectly when he is exposed to an environment contaminated by the urine of the infected animals such as soil or surface water following monsoon rains. Therefore, this illness commonly occurs during the monsoon. These organisms survive for 6 hours in dry soil and for 6 months in flooded conditions. They enter the host through abrasions of the skin (of the feet when they come into contact with infected water) or intact mucus membranes of the eye, throat and gut, when
Iyengar-Yoga Compared to Exercise as a Therapeutic Intervention during (Neo)adjuvant Therapy in Women with Stage I–III Breast Cancer: Health-Related Quality of Life, Mindfulness, Spirituality, Life Satisfaction, and Cancer-Related Fatigue

This study aims to test the effects of yoga on health-related quality of life, life satisfaction, cancer-related fatigue, mindfulness, and spirituality compared to conventional therapeutic exercises during (neo)adjuvant cytotoxic and endocrine therapy in women with breast cancer. In a randomized controlled trial 92 women with breast cancer undergoing oncological treatment were randomly enrolled for a yoga intervention (YI) (n = 45) or for a physical exercise intervention (PEI) (n = 47). Measurements were obtained before (t 0) and after the intervention (t 1) as well as 3 months after finishing intervention (t 2) using standardized questionnaires. Life satisfaction and fatigue improved under PEI (p < 0.05) but not under YI (t 0 to t 2). Regarding quality of life (EORTC QLQ-C30) a direct effect (t 0 to t 1; p < 0.001) of YI was found on role and emotional functioning, while under PEI only emotional functioning improved. Significant improvements (p < 0.001) were observed at both t 1 and t 2 also for symptom scales in both groups: dyspnea, appetite loss, constipation, and diarrhea. There was no significant difference between therapies for none of the analyzed variables neither for t 1 nor for t 2. During chemotherapy, yoga was not seen as more helpful than conventional therapeutic exercises. This does not argue against its use in the recovery phase.
Role of Pro-Inflammatory and Regulatory Cytokines in Pathogenesis of Graves’ Disease in Association with Autoantibody Thyroid and Regulatory FoxP3 T-Cells

Abstract—Background: Graves’ disease (GD) is an autoimmune thyroid disease. Imbalance of Th1/Th2 cells and T-regulatory (Treg)/Th17 cells was thought to play pivotal role in the pathogenesis of GD. Treg FoxP3 produced TGF- to maintain regulatory function, and Th17 cells produced IL-17 as cytokines that were thought in mediating several autoimmune diseases. The aim of this study is to assess the role of IL-17 and TGF- in the pathogenesis of GD and to investigate its correlation with Thyroid Stimulating Hormone Receptor Antibody (TRAb) and Treg FoxP3 expression. Method: 30 GD patients and 27 age and sex-matched controls were enrolled in this study. Diagnosis of GD was based on clinical and biochemical of GD. Serum IL-17, TGF-, TRAb, and FoxP3 were measured by enzyme-linked immunosorbent assay (ELISA). Data were analyzed by using SPSS 21.0 (SPSS Inc.). Spearman rank correlation test was used for assessment of correlation. The statistical significance was accepted as P<0.05. Result: There was no significant correlation between IL-17 and TGF- serum with expression of FoxP3 level in GD, but there was significant correlation between TGF- and TRAb serum level (P<0.05). Serum levels of IL-17 and TGF- were found to be elevated in patient group compared to control, where mean values of IL-17 were 14.432.15 pg/mL and TGF- were 10.443.19 pg/mL in patients group; and in control group, level of IL-17 were 7.11.45 pg/mL and TGF- were 4.951.35 pg/mL. Conclusion: Serum Il-17 and TGF- were elevated in GD patients that reflect the role of inflammatory and regulatory cytokines activation in pathogenesis of GD. There was significant correlation between TGF and TRAb, revealing that Treg cytokines may play a role in pathogenesis of GD.
[Effect of continuous hemofiltration on inflammatory mediators and hemodynamics in children with severe hand, foot and mouth disease].

OBJECTIVE To investigate the effect of continuous veno-venous hemofiltration (CVVH) on inflammatory mediators in children with severe hand, foot and mouth disease (HFMD), and to investigate its clinical efficacy. METHODS A total of 36 children with stage IV HFMD were enrolled and randomly divided into conventional treatment group and CVVH group (n=18 each). The children in the CVVH group were given CVVH for 48 hours in addition to the conventional treatment. The levels of interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α) and lactic acid in peripheral venous blood, heart rate, blood pressure, and left ventricular ejection fraction were measured before treatment and after 24 and 48 hours of treatment. RESULTS After 24 hours of treatment, the conventional treatment group had a significantly reduced serum IL-2 level (P<0.01), and the CVVH treatment group had significantly reduced serum levels of IL-2, IL-6, IL-10, and TNF-α (P<0.05). After 48 hours of treatment, both groups had significantly reduced serum levels of IL-2, IL-6, IL-10, and TNF-α (P<0.01), and the CVVH group had significantly lower levels of these inflammatory factors than the conventional treatment group (P<0.01). After 48 hours of treatment, heart rate, systolic pressure, and blood lactic acid level were significantly reduced, and left ventricular ejection fraction was significantly increased in both groups, and the CVVH group had significantly greater changes in these indices except systolic pressure than the conventional treatment group (P<0.01). CONCLUSIONS CVVH can effectively eliminate inflammatory factors, reduce heart rate and venous blood lactic acid, and improve heart function in children with severe HFMD.
Análisis Para El Diseño De Un Silo De Clinker Y Su Implementación Computacional

El objetivo fundamental del presente trabajo consiste en efectuar el estudio de un proyecto correspondiente a un silo de clinker, tipologia estructural de gran aplicacion en las industrias de proceso continuo, como es el caso de la operatoria de las industrias cementeras. El problema tratado abarca varios aspectos: estudio teorico del problema, implementacion del modelo computacional, interpretacion de resultados; contando con la documentacion correspondiente al proyecto del mismo. Para el diseno se tuvieron en cuenta las disposiciones del Codigo ACI 313 y el Reglamento CIRSOC, dada la exigencia de cumplimentar algunos requisitos para el silo en cuestion, de manera de permitir que la planta del mismo sea tal que permita que el material ensilado pierda temperatura y debe tambien cumplir la condicion de estanqueidad, a los fines de que el material ensilado no entre en contacto con la humedad. Los resultados alcanzados, a traves de la implementacion del modelo computacional, se encuentran en una satisfactoria aproximacion con los resultados obtenidos de la aplicacion de la teoria clasica. Asimismo del analisis computacional efectuado se visualiza la importancia relevante que guardan consigo las condiciones de borde, las cuales deben representar lo mas fielmente a las disenadas para la estructura en cuestion.
Information Communities: The Network Structure of Communication

This study puts forward a variable clique overlap model for identifying information communities, or potentially overlapping subgroups of network actors among whom reinforced independent links ensure efficient communication. We posit that the intensity of communication between individuals in information communities is greater than in other areas of the network. Empirical tests show that the variable clique overlap model is more useful for identifying groups of individuals that have strong internal relationships in closed networks than those defined by more general models of network closure. These findings extend the scope of network closure effects proposed by other researchers working with communication networks using social network methods and approaches, a tradition which emphasizes ties between organizations, groups, individuals, and the external environment.
Capacity conservation ratio: a novel interference constraint for spectrum sharing

This paper presents an interference constraint and power control schemes based on the capacity conservation ratio (CCR). The CCR is the ratio of the decreased capacity in the spectrum sharing to the original capacity of a system. By utilising the CCR as a metric to protect the primary user, a secondary user can achieve sufficient performance, without a large degradation in the primary capacity. In this paper, we propose novel power control schemes under interference constraints based on the CCR, with consideration of the fading effect, by using theoretical analysis. There are two types of power control schemes, namely, an exact power control scheme (EPCS) and a simplified power control scheme (SPCS). The EPCS can control the outage probability; however, a numerical analytic approach is necessary to determine the transmit power. In contrast, the SPCS can be used to derive the transmit power from a closed form with an archiving requirement of the constraint. We analytically derive the average capacity of the primary user and secondary user and the complementary cumulative distribution function of the primary capacity. From these numerical results, we show that the interference constraint based on the CCR achieves better performance in underlay spectrum sharing than the other two interference constraints. Furthermore, the SPCS can achieve a performance equivalent to that of the EPCS, and the SPCS can protect both the low and the high primary capacities under the same constraint. Copyright © 2013 John Wiley & Sons, Ltd.
Visual saliency on networks of neurosynaptic cores

Identifying interesting or salient regions in an image plays an important role for multimedia search, object tracking, active vision, segmentation, and classification. Existing saliency extraction algorithms are implemented using the conventional von Neumann computational model. We propose a bottom-up model of visual saliency, inspired by the primate visual cortex, which is compatible with TrueNorth-a low-power, brain-inspired neuromorphic substrate that runs large-scale spiking neural networks in real-time. Our model uses color, motion, luminance, and shape to identify salient regions in video sequences. For a three-color-channel video with 240 $\times$ 136 pixels per frame and 30 frames per second, we demonstrate a model utilizing $\sim$ 3 million neurons, which achieves competitive detection performance on a publicly available dataset while consuming $\sim$ 200 mW.
Increased endothelin and endothelin receptor mRNA expression in polycystic kidneys of cpk mice.

The renal mRNA levels of endothelin (ET)-1 and ET-3 and for ET receptors A and B were measured in the cystic kidneys of cpk/cpk mice at 1, 2, and 3 wk of age. At 1 wk of age, renal ET-1 mRNA was 3.2-fold greater in cystic mice than in controls and continued to increase with the progression of cyst formation to reach 10.4-fold more than controls at 3 wk. ET-3 mRNA levels did not differ between cystic and control mice. Renal ETA and ETB receptor mRNA increased gradually in cystic mice with the progression of their cysts, reaching 4.2- and 6.3-fold increases over controls, respectively, at 3 wk. Proliferating cell nuclear antigen mRNA expression was also examined, and proliferating cell nuclear antigen mRNA levels were found to be significantly increased in the kidneys of cystic mice compared with controls: 2. 1-fold at 1 wk, 4.5-fold at 2 wk, and 7.8-fold at 3 wk. The mRNA levels for transforming growth factor beta (TGF-beta) and tumor necrosis factor alpha (TNF-alpha) in the kidneys of cystic mice were also examined and were found to be increased progressively with age (TGF-beta, 2.1-fold at 1 wk, 4.2-fold at 2 wk, and 6.2-fold at 3 wk; TNF-alpha, 2.2-fold at 1 wk, 3.8-fold at 2 wk, and 5.4-fold at 3 wk). (ABSTRACT TRUNCATED AT 250 WORDS)
Design of a G-quadruplex topology through glycosidic bond angles.

To realize a programmed build up of DNA objects, devices,and materials, a systematization of the principles that formthe basis of control for the assembly process is necessary. Thusfar the discovery of four-stranded DNA architecturesdenominated G quadruplexes was either serendipitous orthrough coincidental emergence. Herein we utilize a rationalapproach for the design of G quadruplexes based on the twostatedisposition of the glycosidic bond angle.
Recherche de biomarqueurs de la neurotoxicité des traitements anticancéreux à base d'oxaliplatine: approche protéomique quantitative

L'oxaliplatine est un medicament de reference dans les traitements des cancers colorectaux metastatiques. Toutefois, l'oxaliplatine occasionne une toxicite d'ordre neurologique qui retentit sur la qualite de vie de certains patients predisposes. Nous avons analyse pour la premiere fois la neurotoxicite de l'oxaliplatine par une approche proteomique. Un protocole experimental, associant marquage iTRAQ et fractionnement par IEFOFFGEL a tout d'abord ete mis en place afin d'ameliorer la couverture proteomique d'echantillons complexes. Puis, l'expression proteique differentielle apres traitement par I'oxaliplatine a ete analysee globalement, a la fois dans le proteome intracellulaire et dans le secretome d'un modele cellulaire humain de nerotoxicite. L'analyse du proteome intracellulaire a permis l'identification de plus de 2700 proteines et offre de nouvelles perspectives quant aux mecanismes moleculaires de la neurotoxicite de I ' o x aliplatine. Le medicament aItere l'expression de proteines impliquees dans la reponse aux dommages a l'ADN, le controle du cycle cellulaire, le stress oxydatif , le metabolisme energetique, le stress proteotoxique, la resistance multidrogue, la plasticite neuronale et l'homeostasie calcique. L'analyse du secretome a mis en evidence la sur-expression, suite au traitement par l'oxaliplatine, de 23 proteines secretees. Nous proposons pour la premiere fois les proteines Calmoduline, Thymosine beta-10 et le facteur neurotrophique Neudesine comme candidats biomarqueurs de la neurotoxicite des traitements anticancereux a base d'oxaliplatine.
Recent advances in the social and developmental biology of the myxobacteria.

INTRODUCTION . 71 MOLECULAR PHYLOGENY.71 SIGNALING . 71 A Signal . 71 B Signal . 74 C Signal.74 D Signal.75 E Signal . 75 Summary of Signaling.76 OTHER EXTRACELLULAR DEVELOPMENTAL FACTORS . 76 Glucosamine, Phospholipase, Glycerol, and Autocides . 76 INITIATION SIGNAL.77 EXTRACELLULAR APPENDAGES . 77 Pili.77 Fibrils . 77 FRUITING BODY FORMATION . 79 Cell Density . 79 Role of Motility . 79 Aggregation . 80 Peripheral Cells . 80 Fruiting Body Morphogenesis.81 DEVELOPMENTAL AUTOLYSIS . 82 TACTIC BEHAVIOR.83 Chemotaxis . 83 Tactic Perception of Physical Objects.85 MOLECULAR BIOLOGY . 85 RETRONS.86 PHASE VARIATION . 86 REGULATION AND SIGNAL TRANSDUCTION.88 Transcriptional Regulation.88 Regulatory Sites . 89 Sigma Factors and Protein Kinases.90 RIPPLING . 90 MOTILITY.91 CAROTENOIDS AND LIGHT.93 PROTEIN SECRETION . 93 ANTIBIOTICS.95 MYXOSPORE MORPHOGENESIS . 96 GLYCEROL SPORES.96 TERRITORIALITY.96 MATHEMATICAL MODELING.97 MYXOSPORE GERMINATION . 97 STIGMATELLA AURANTIACA . 97 EUKARYOTE-LIKE PROPERTIES OF MYXOBACTERIA.97 EPILOGUE.98 ACKNOWLEDGMENTS . 98 REFERENCES . 98
Early endosomal rerouting of major histocompatibility class I conformers

Major histocompatibility class I (MHC‐I) molecules are present at the cell surface both as fully conformed trimolecular complexes composed of heavy chain (HC), beta‐2‐microglobulin (β2m) and peptide, and various open forms, devoid of peptide and/or β2m (open MHC‐I conformers). Fully conformed MHC‐I complexes and open MHC‐I conformers can be distinguished by well characterized monoclonal antibody reagents that recognize their conformational difference in the extracellular domain. In the present study, we used these tools in order to test whether conformational difference in the extracellular domain determines endocytic and endosomal route of plasma membrane (PM) proteins. We analyzed PM localization, internalization, endosomal trafficking, and recycling of human and murine MHC‐I proteins on various cell lines. We have shown that fully conformed MHC‐I and open MHC‐I conformers segregate at the PM and during endosomal trafficking resulting in the exclusion of open MHC‐I conformers from the recycling route. This segregation is associated with their partitioning into the membranes of different compositions. As a result, the open MHC‐I conformers internalized with higher rate than fully conformed counterparts. Thus, our data suggest the existence of conformation‐based protein sorting mechanism in the endosomal system. J. Cell. Physiol. 227: 2953–2964, 2012. © 2011 Wiley Periodicals, Inc.
Estudio de la utilidad clínica de la cuantificación de arn y adn plasmáticos en pacientes con cáncer de laringe e hipofaringe

El objetivo general del estudio fue evaluar la utilidad clinica de la deteccion y cuantificacion de ARN y ADN en el plasma de pacientes intervenidos de cancer de laringe e hipofaringe. Especificamente se evaluo el posible valor diagnostico de la cuantificacion del ARNm de la subunidad hTERT de la telomerasa, del ARNm de la GAPDH y del ADN en plasma, asi como la correlacion entre dichos niveles y las distintas variables anatomoclinicas, y su valor pronostico.
Effective pore‐scale dispersion upscaling with a correlated continuous time random walk approach

We investigate the upscaling of dispersion from a pore‐scale analysis of Lagrangian velocities. A key challenge in the upscaling procedure is to relate the temporal evolution of spreading to the pore‐scale velocity field properties. We test the hypothesis that one can represent Lagrangian velocities at the pore scale as a Markov process in space. The resulting effective transport model is a continuous time random walk (CTRW) characterized by a correlated random time increment, here denoted as correlated CTRW. We consider a simplified sinusoidal wavy channel model as well as a more complex heterogeneous pore space. For both systems, the predictions of the correlated CTRW model, with parameters defined from the velocity field properties (both distribution and correlation), are found to be in good agreement with results from direct pore‐scale simulations over preasymptotic and asymptotic times. In this framework, the nontrivial dependence of dispersion on the pore boundary fluctuations is shown to be related to the competition between distribution and correlation effects. In particular, explicit inclusion of spatial velocity correlation in the effective CTRW model is found to be important to represent incomplete mixing in the pore throats.
Simulation and analysis of variable step size P&O MPPT algorithm for photovoltaic power control

The Maximum Power point Tracking Controller (MPPT) is an essential part of the photovoltaic (PV) system, it is used to extract the maximum power from the photovoltaic solar module and transfer that power to the load. Several MPPT techniques have been proposed in the literature. One of the most commonly used techniques is the perturb and observe algorithm (P&O), due to its low cost and simple implementation, but it suffers from the slow tracking speed for small step of perturbation and oscillations around the maximum power point (MPP) when it is subjected to a large step. In this article, the P&O algorithm with a variable step size (VS_P&O) is simulated by the PSIM software, analyzed and compared with the fixed step size P&O technique (FS_P&O). These two techniques are also simulated with and without proportional integrator corrector (PI corrector). The results show the good tracking, the high efficiency and precision of the VS_P&O technique using the PI corrector.
CNT/PDMS-based canal-typed ear electrodes for inconspicuous EEG recording

Objective. Current electroencephalogram (EEG) monitoring systems typically require cumbersome electrodes that must be pasted on a scalp, making a private recording of an EEG in a public place difficult. We have developed a small, user friendly, biocompatible electrode with a good appearance for inconspicuous EEG monitoring. Approach. We fabricated carbon nanotube polydimethylsiloxane (CNT/PDMS)-based canal-type ear electrodes (CEE) for EEG recording. These electrodes have an additional function, triggering sound stimulation like earphones and recording EEG simultaneously for auditory brain–computer interface (BCI). The electrode performance was evaluated by a standard EEG measurement paradigm, including the detection of alpha rhythms and measurements of N100 auditory evoked potential (AEP), steady-state visual evoked potential (SSVEP) and auditory steady-state response (ASSR). Furthermore, the bio- and skin-compatibility of CNT/PDMS were tested. Main results. All feasibility studies were successfully recorded with the fabricated electrodes, and the biocompatibility of CNT/PDMS was also proved. Significance. These electrodes could be used to monitor EEG clinically, in ubiquitous health care and in brain–computer interfaces.