Miracle-dz/UniFAR-FTUs huggingface dataset:
Focal Inner Retinal Hemorrhages in Patients with Drusen: An Early Sign of Occult Choroidal Neovascularization and Chorioretinal Anastomosis

Purpose To present evidence that superficial retinal hemorrhage in the macula of patients with age-related macular degeneration (ARMD) may be an early sign of occult chorioretinal anastomosis (OCRA) and type 1 occult choroidal neovascularization (OCNV). Methods Retrospective follow-up study of 16 patients presenting with a small focal area of superficial retinal hemorrhages and drusen in the juxtafoveolar area in 24 eyes. Results OCRA and OCNV occurred in an older subset of patients with ARMD (mean age, 75 years). Of 22 eyes with the early stages of chorioretinal anastomosis (CRA), 18 had evidence of a piggyback neovascular complex, with the smaller subsensory retinal type 2 complex lying anterior to the larger subretinal pigment epithelial type 1 complex. At initial presentation, three patients had OCRA and OCNV bilaterally, and three patients had large disciform cicatricial lesions with overt CRA in the fellow eye. Nine patients had one or more laser photocoagulation treatments for early stages of CRA. Only one patient maintained visual acuity of better than 20/200 for >1 year. At the last follow-up, 24 of 26 eyes with CRA had visual acuity of 20/200 or less. Conclusion Superficial retinal hemorrhage in the paracentral area of patients with drusen is the earliest sign of OCRA and OCNV. Fluorescein angiography and indocyanine green angiography are important in detecting the dual nature of the subretinal neovascular network. Photocoagulation and photodynamic treatment is usually unsuccessful in preserving central vision.
Seasonal Abundance of Total and Pathogenic Vibrio parahaemolyticus in Alabama Oysters

ABSTRACT Recent Vibrio parahaemolyticus outbreaks associated with consumption of raw shellfish in the United States focused attention on the occurrence of this organism in shellfish. From March 1999 through September 2000, paired oyster samples were collected biweekly from two shellfish-growing areas in Mobile Bay, Ala. The presence and densities of V. parahaemolyticus were determined by using DNA probes targeting the thermolabile hemolysin (tlh) and thermostable direct hemolysin (tdh) genes for confirmation of total and pathogenic V. parahaemolyticus, respectively. V. parahaemolyticus was detected in all samples with densities ranging from <10 to 12,000 g−1. Higher V. parahaemolyticus densities were associated with higher water temperatures. Pathogenic strains were detected in 34 (21.8%) of 156 samples by direct plating or enrichment. Forty-six of 6,018 and 31 of 6,992 V. parahaemolyticus isolates from enrichments and direct plates, respectively, hybridized with the tdh probe. There was an apparent inverse relationship between water temperature and the prevalence of pathogenic strains. Pathogenic strains were of diverse serotypes, and 97% produced urease and possessed a tdh-related hemolysin (trh) gene. The O3:K6 serotype associated with pandemic spread and recent outbreaks in the United States was not detected. The efficient screening of numerous isolates by colony lift and DNA probe procedures may account for the higher prevalence of samples with tdh+V. parahaemolyticus than previously reported.
p66/p51 and p51/p51 recombinant forms of reverse transcriptase from human immunodeficiency virus type 1--interactions with primer tRNA(Lys3), initiation of cDNA synthesis, and effect of inhibitors.

Human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) initiates reverse transcription from tRNA(Lys3). HIV-1 RT is a heterodimer consisting of two polypeptides, p66 and p51. In this work, the possible role of each subunit of RT in the interaction with its natural primer tRNA(Lys3) was studied. Two recombinant forms of HIV-1 RT, heterodimer p66/p51 and homodimer p51/p51, were used. Previously we have expressed and purified recombinant RT p51/p51 which possesses DNA polymerase activity [El Dirani-Diab, R. , Andreola, M. L. , Nevinsky, G. , Tharaud, D. , Barr, P. J., Litvak, S. & Tarrago-Litvak, L. (1992) FEBS Lett. 301, 23-28]. Here we show that HIV-1 RT p51/p51 displays certain properties very similar to the p66/p51 recombinant enzyme. The homodimer was able to anneal tRNA(Lys3) to the primer-binding site of the HIV-1 RNA template leading to a functional complex capable of synthesizing cDNA. Further, the p51/p51 enzyme behaved like RT p66/p51 concerning the strong inhibition produced by a non-nucleoside RT inhibitor. These data show that for RT p51/p51, one of the subunits of the homodimer adopts a conformation similar to the catalytic subunit (p66) present in the heterodimeric form. Part of this work was devoted to the study of the complex between the recombinant forms of HIV-1 RT and its primer tRNA. Each enzymatic form was cross-linked to tRNA(Lys3) in the presence of a platinum derivative, giving different ribonucleoprotein complexes of molecular masses higher than 100 kDa, suggesting that primer tRNA may interact with both subunits in the heterodimeric enzyme. After RNase A treatment of the complex RT p66/p51 x tRNA, the label was mainly found to migrate with the p66 subunit, although some cross-linking was also found associated to the p51 subunit. These results show that the p66 and p51 subunits of RT interact with tRNA(Lys3). Moreover, cross-linking of tRNA(Lys3) with HIV-1 RT p66/p51 in the presence of a DNA template containing the primer-binding-site sequence yielded an enzymatically active complex.
The Influence of Optimal Handheld Load on the Technical Ability to Apply Ground Reaction Forces during Horizontal Jumping in Female Netball Players

Abstract Handheld load has been reported to enhance horizontal jump performance, however little is known about its influence on ground reaction forces (GRF), especially in female athletes. This study investigated the effects of individualized optimal handheld loading on the technical and physical ability to apply GRF during horizontal jumping in female netball players. Maximal effort, single standing, horizontal jumps were performed by 13 female netballers. Participants performed the jumps under 2 conditions: 1) unloaded, and 2) loaded. Eccentric mean horizontal GRF significantly increased with loading (p<0.05; Effect Size [ES]= 0.74). The ratio of horizontal-to-total GRF significantly increased (p<0.05; ES=0.57), however resultant GRF did not, suggesting that the technical ability to apply force in the direction of intended movement may be of greater importance than the magnitude of force applied. Jump distance also increased from 188.2±16.1 cm to 196.4±13.6 cm (p<0.01; ES=0.55) with handheld load. In conclusion, individualized optimal handheld loading improved single horizontal jump performance in this population of athletes; most likely through various mechanisms that allowed for increased eccentric horizontal GRFs and the technical ability of force application. Findings could have practical implications for the strength and conditioning coach, trainer and athlete.
Fast food consumption and increased caloric intake: a systematic review of a trajectory towards weight gain and obesity risk

Consumption of fast food, which have high energy densities and glycemic loads, and expose customers to excessive portion sizes, may be greatly contributing to and escalating the rates of overweight and obesity in the USA. Whether an association exists between fast food consumption and weight gain is unclear. Sixteen studies (six cross sectional, seven prospective cohort, three experimental) meeting methodological and relevance criteria were selected for inclusion in this systematic review. While more research needs to be conducted specifically in regard to effects of fast food consumption among subpopulations such as children and adolescents, sufficient evidence exists for public health recommendations to limit fast food consumption and facilitate healthier menu selection. As the fast food industry continues to increase both domestically and abroad, the scientific findings and corresponding public health implications of the association between fast food consumption and weight are critical.
TRUSTED COMPUTER SYSTEMS

The DoD has established a Computer Security Initiative to foster the wide-spread availability of trusted computer systems. An essential element of theXnitiative is the identification of criteria and guidelines for evaluating the internal protection mechanisms of computer systems. This report documents a proposed set of technical evaluation criteria. These criteria and any evaluation process that they might imply represent one approach to how trusted systems might be evaluated. The information in this report is made available to stimulate technical discussion among industry and government personnel. Procedures or cr±teria formally coordinated and adopted by the Department of Defense in the future may differ from those proposed here. The views and conclusions contained in this paper are those of the author and should not be interpreted as representing the official policies, either expressed or implied, of the Department of Defense or the United States Government. "-•I i
Polypharmacy in the elderly: A literature review

Purpose To review the body of literature addressing polypharmacy in individuals aged 60 years and older to (a) determine primary care providers’ definition of polypharmacy, (b) explore how polypharmacy was assessed in primary care, and (c) seek tested interventions that address polypharmacy. Data sources A systematic review of electronic bibliographic databases (e.g. EBSCOHost, InfoTrac, OVID, FirstSearch, and FirstSearch Deluxe) utilizing the search terms “polypharmacy,”“polypharmacy and elderly,”“polypharmacy and research,” and “multiple medications” for the period January 1991 to October 2003 was completed. The search was supplemented with online site searches of relevant journals and review of reference lists of each article. Conclusions Results of the literature review revealed that polypharmacy continues to be a significant issue and little research has been conducted regarding the methods primary care providers utilize to assess polypharmacy. Also, there is a gap in the literature regarding the interventions implemented by primary care providers to address polypharmacy. Implications for practice The following definition of polypharmacy in clinical practice might be more practical: the use of medications that are not clinically indicated. Selecting appropriate limits for numbers of medications may be counterproductive in populations with multiple comorbidities.
Clustering of significant genes in prognostic studies with microarrays: Application to a clinical study for multiple myeloma

When a large number of genes are significant in correlating microarray gene expression data with patient prognosis, clustering of significant genes may be effective not only for further dimension reduction but also for identifying co‐regulated genes that belong to the same molecular pathway related to disease biology and aggressiveness. Moreover, a reduced feature, such as the average expression across samples for a cluster of significant genes, can play an important role in reducing variance in prediction analysis. We propose a simple procedure to select gene clusters that have strong marginal association with survival outcome from a large pool of candidate hierarchical clusters of significant genes. Selected gene clusters can have better predictive capability than the other gene clusters and singleton genes. Application of such clustering to the data set from a clinical study for patients with multiple myeloma and associated microarrays is given. Copyright © 2007 John Wiley & Sons, Ltd.
Medication used in Nausea and Vomiting of Pregnancy - A Review of Safety and Efficacy

Nausea and vomiting are common symptoms experienced by 50– 90% of women’s in early pregnancy. ‘Morning sickness’ is a misnomer frequently used to describe nausea and vomiting in pregnancy (NVP), although the symptoms may persist the whole day and/or night. Pregnant women experience these symptoms mainly in the first trimester between 6 and 12 weeks of gestation, few of them continue till 20 weeks of gestation while in few others it continues throughout the pregnancy. The problem peaks at 9-week gestation, and approximately 60% of NVPs resolve by the end of first trimester. In a very small minority of these patients, the symptoms become severe leading to dehydration, weight loss, excessive vomiting, and mandate hospital admission; this condition is known as Hyperemesis Gravidarum. Fairweather D.V proposed the most widely used definition of Hyperemesis Gravidarum (HG). He defined HG based on the symptoms, vomiting exceeding three times a day with significant ketonurea or weight loss more than or equal to 5% of pre pregnancy weight, electrolytic imbalance or fluid depletion, and onset occurs at 4 to 8 weeks of pregnancy till 14 to 16 weeks. Nausea and vomiting in pregnancy is of multifarious etiology (fluctuating levels of progesterone, estrogens, Thyroid Stimulating Hormone (TSH), slow peristaltic movement of Gastrointestinal (GI) tract); however, the exact mechanism remains still unclear. Given the uncertainty in treatment of NVPs, both patients and healthcare practitioners often fear the use of antiemetic medications in pregnancy due to the potential risk to fetus and mother. The manifestation of nausea and vomiting in pregnancy is different among each woman, so its management should be tailored similarly. An early treatment of nausea and vomiting is important and beneficial since it prevents a more severe form of occurring, or a possible hospitalization, and prevents both emotional and psychological problems. It is very important for the women and the healthcare providers to understand that a safe and effective NVP treatment benefits both fetus and mother, thus all the treatment options should be open and considered. Nonetheless, given the widespread prevalence of nausea and vomiting, its adverse effects and effects on psychological conditions of pregnant women, it is necessary to be treated effectively and safely during embryonic and fetal developmental stages. First trimester exposure is important to be assessed to monitor the teratogenic potential of the drug; however, randomized control trials are rarely conducted for pregnant women for ethical reasons. Whereas the epidemiological studies done are observational and lack population strength to establish safety and risk involved. This review will mainly focus on pharmacological drugs used in treatment of NVP, and explore their safety and efficacy and evidence based practice. There have been many studies examining the safety of drugs used in NVPs and few of them are covered in this review. The dietary, lifestyle modifications, and nonpharmacological approaches are not covered in this section.
Serum lysyl oxidase activity in chronic liver disease in comparison with serum levels of prolyl hydroxylase and laminin

Lysyl oxidase was partially purified from serum by a diethylaminoethyl batch procedure in the presence of 6 mom/L urea and dialyzed against 3 mol/L KSCN. Using this method, we determined serum lysyl oxidase activity in 52 patients with liver disease and in 14 healthy controls, and we examined usefulness of serum lysyl oxidase in assessing liver fibrogenesis. For this purpose, serum lyayl oxidase activity in chronic liver disease was compared with serum levels of prolyl hydroxylase and laminin P1. As compared with controls, serum lysyl oxidase activity increased 1.6‐fold in chronic persistent hepatitis, 4.4‐fold in chronic active hepatitis and 11.8‐fold in cirrhosis, indicating an increase in concert with the development of liver fibrosis. In hepatocellular carcinoma, the serum activity, although signiscantly increased, was lower than that in cirrhosis. Serum prolyl hydroxylase was significantly increased in chronic active hepatitis, in liver cirrhosis and in hepatocellular carcinoma Serum laminin P1 was significantly increased in chronic active hepatitis, in cirrhosis and in hepatocellular carcinoma Serum lysyl oxidase activity did not correlate significantly with serum levels of prolyl hydroxylase and laminin P1 in any subject or in any subgroup. The magnitude of the increase and the abnormal percentage of serum lysyl oxidase activity were larger than those for serum prolyl hydroxylase and laminin P1. These results suggest that serum lysyl osidase activity is a more sensitive indicator of liver fibrosis than serum prolyl hydroxylase and laminin P1. (HEPATOLOGY 1991;14:1167–1173.)
¿Nei takk=no gracias? Agradecimientos positivos y negativos: hacia un estudio comparativo de las reglas de cortesía español-noruego y su tratamiento en los manuales de español para estudiantes noruegos

Introduccion Esta exposicion pretende hacer una reflexion sobre lo que supone una optima competencia comunicativa, prestando especial atencion a la union entre cultura y lengua; en otras palabras, a la competencia pragmatica o sociolinguistica y a la importancia de la misma en la ensenanza de una lengua extranjera desde niveles iniciales. Nuestro objeto de interes comprende tambien analizar analogias y diferencias respecto a las convenciones culturales noruego/espanol en actos de habla como agradecer, rechazar o aceptar alguna invitacion u ofrecimiento; es decir; agradecimientos positivos y negativos y la adecuacion y representacion de los mismos en los manuales de espanol para alumnos noruegos. Con el enfoque comunicativo surge el concepto de competencia comunicativa acunado por Hymes desde la etnografia de la comunicacion, cuyo modelo de analisis puede resumirse en su formula mnemotecnica SPEAKING; es decir, habria que tener en cuenta la situacion espacio-temporal, los participantes, los propositos y objetivos de un acto de habla, la secuencia de actos, los elementos verbales y no verbales, los instrumentos utilizados, las normas de interaccion e interpretacion en las que el conocimiento de ellas implica el conocimiento de la estructura social y de relaciones sociales concretas, con especial relevancia en actos de habla con participantes pertenecientes a distintas comunidades y los generos, que desde el punto de vista sociocultural se asocian a determinados usos linguisticos. Con este modelo Hymes enfatiza aspectos sociales del habla y el contexto donde tienen lugar (Del Olmo Pintado 2004: 166-172).
Increased complexity of radiation-induced chromosome aberrations consistent with a mechanism of sequential formation

Complex chromosome aberrations (any exchange involving three or more breaks in two or more chromosomes) are effectively induced in peripheral blood lymphocytes (PBL) after exposure to low doses (mostly single particles) of densely ionising high-linear energy transfer (LET) α-particle radiation. The complexity, when observed by multiplex fluorescence in situ hybridisation (m-FISH), shows that commonly four but up to eight different chromosomes can be involved in each rearrangement. Given the territorial organisation of chromosomes in interphase and that only a very small fraction of the nucleus is irradiated by each α-particle traversal, the aim of this study is to address how aberrations of such complexity can be formed. To do this, we applied theoretical “cycle” analyses using m-FISH paint detail of PBL in their first cell division after exposure to high-LET α-particles. In brief, “cycle” analysis deconstructs the aberration “observed” by m-FISH to make predictions as to how it could have been formed in interphase. We propose from this that individual high-LET α-particle-induced complex aberrations may be formed by the misrepair of damaged chromatin in single physical “sites” within the nucleus, where each “site” is consistent with an “area” corresponding to the interface of two to three different chromosome territories. Limited migration of damaged chromatin is “allowed” within this “area”. Complex aberrations of increased size, reflecting the path of α-particle nuclear intersection, are formed through the sequential linking of these individual sites by the involvement of common chromosomes.
Study on attitudes of students of Islamic Azad University Tehran Medical Branch toward food safety, 2016

Background Given the importance of high-quality healthy food for humans, contamination control is the most important concern for healthy staff. Aim To determine the attitudes of students at Islamic Azad University (Tehran Medical Branch) toward food safety. Methods This cross-sectional and analytic-descriptive study was conducted on 326 students of Azad University of Medical Sciences in 2016. A self-made questionnaire consisting of 40 questions was used. The reliability of the questionnaire was confirmed using internal consistency method (Cronbach’s alpha coefficient of 0.80). After collecting data, we use descriptive statistical indexes (mean and standard deviation) among demographic variables and the level of knowledge to describe and analyze the data. The participants’ attitudes and operation are measured by Spearman tests, and the analytical results are given using SPSS version 20. Results According to the findings, 55.3, 30 and 14.7 percent of students had high, moderate and low attitude scores toward food safety, respectively. In addition, male and female students had equal attitudes toward food safety, and no significant relationship between sex and attitude was observed a significant difference (p>0.05), but between educational levels (p=0.008) and ages (p=0.001) of students significance was a positive correlation. Conclusions Due to the low attitudes score of about half of the students of Islamic Azad University Tehran Medical Branch toward food safety, it can be claimed, food safety training in this community is required.
The Molecular Chaperone Calnexin Binds Glc1Man9GlcNAc2 Oligosaccharide as an Initial Step in Recognizing Unfolded Glycoproteins (*)

Calnexin is a molecular chaperone that resides in the membrane of the endoplasmic reticulum. Most proteins that calnexin binds are N-glycosylated, and treatment of cells with tunicamycin or inhibitors of initial glucose trimming steps interferes with calnexin binding. To test if calnexin is a lectin that binds early oligosaccharide processing intermediates, a recombinant soluble calnexin was created. Incubation of soluble calnexin with a mixture of Glc0–3Man9GlcNAc2 oligosaccharides resulted in specific binding of the Glc1Man9GlcNAc2 species. Furthermore, Glc1Man5–7GlcNAc2 oligosaccharides bound relatively poorly, suggesting that, in addition to a requirement for the single terminal glucose residue, at least one of the terminal mannose residues was important for binding. To assess the involvement of oligosaccharide-protein interactions in complexes of calnexin and newly synthesized glycoproteins, α1-antitrypsin or the heavy chain of the class I histocompatibility molecule were purified as complexes with calnexin and digested with endoglycosidase H. All oligosaccharides on either glycoprotein were accessible to this probe and could be removed without disrupting the association with calnexin. Furthermore, the addition of 1 M α-methyl glucoside or α-methyl mannoside had no effect on complex stability. These findings suggest that once complexes between calnexin and glycoproteins are formed, oligosaccharide binding does not contribute significantly to the overall interaction. However, it is likely that the binding of Glc1Man9GlcNAc2 oligosaccharides is a crucial event during the initial recognition of newly synthesized glycoproteins by calnexin.