Miracle-dz/UniFAR-FTUs huggingface dataset:
Safety Profile and Efficacy of Chemoembolization with Doxorubicin - Loaded Polyethylene Glycol Microspheres in Patients with Hepatocellular Carcinoma

AIM: This study was designed as a preliminary investigation of safety and efficacy of LifePearl, polyethylene glycol microspheres loaded with doxorubicin for treatment of locally untreatable (i.e., unresectable and not suitable for local thermal ablation) hepatocellular carcinoma (HCC). MATERIAL AND METHODS: Patients with locally untreatable HCC (mono- or bilobar disease, ECOG performance status 0-1, Chilg-Pugh score < 11) were analysed for this single arm Unicenter retrospective study. All the information were acquired through our local hospital information system. DEB-TACE was performed with 100-200 microns LifePearl loaded with 75-150 mg of doxorubicin depending on tumour size. One interventional radiologist with experience of more than 350 TACE procedures and one fellow in radiology performed all embolisations. RESULTS: Twenty subjects with 29 tumours were treated (mean age 66.2 years). Child-Pugh status was A for 12 pts. (60%), B for 6 pts. (30%) and C for 2 pts. (10%). Three patients had insignificant ascites. Most patients (70%) underwent < 3 DEB-TACE procedures. Average doxorubicin dose was 71.1 mg per procedure. One patient had procedure-related SAE (acute pancreatitis) within the postembolization period which was induced due to non-target embolisation of the superior pancreaticoduodenal artery. Six-month freedom from procedure-related SAE or death was 95% (one necrotizing pancreatitis). Tumor response or stable disease was achieved in 95% (19/20) of subjects. Freedom from tumor progression or death at 6 months was 95%. One-year survival rate was 90% overall. CONCLUSION: The results from this investigation suggest that LifePearl microspheres, Terumo loaded with doxorubicin can provide an excellent local tumour control with very few side effects in a relatively homogeneous group of patients with locally untreatable HCC.
The Role of Human Milk in Decreasing Necrotizing Enterocolitis Through Modulation of the Infant Gut Microbiome: A Scoping Review

Background Necrotizing enterocolitis is associated with a high incidence of morbidity and mortality in premature infants. Human milk minimizes necrotizing enterocolitis risk, although the mechanism of protection is not thoroughly understood. Increasingly, dysbiosis of the infant gut microbiome, which is affected by infant diet, is hypothesized to play a role in necrotizing enterocolitis pathophysiology. Research aim The aim of this scoping review was to summarize the state of the science regarding the hypothesis that the gut microbiome composition is a mediator of the relationship between human milk and decreased incidence of necrotizing enterocolitis within a sample of human infants. Methods Electronic databases and reference lists were searched for peer-reviewed primary research articles addressing the link between human milk, gut microbiome composition, and subsequent incidence of necrotizing enterocolitis among human infants. Results A total of four studies met criteria for inclusion in this review. Of these, evidence supporting the link between human milk, gut microbiome composition, and necrotizing enterocolitis was found in two (50%) studies. Conclusion Some evidence linking all three variables is provided in this review. Given the small number of available studies, and the limitations of those studies, more research is urgently needed to thoroughly understand the protection against necrotizing enterocolitis gained through the provision of human milk.
A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging

The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound YZ-A4 as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor YZ-A4 to selenocysteine was from 0 to 32 μM, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that YZ-A4 could be used for fluorescence imaging of selenocysteine in living cells.
Patterns of Postpartum Ambulatory Care Follow‐up Care Among Women With Hypertensive Disorders of Pregnancy

Background Preeclampsia and gestational hypertension are hypertensive disorders of pregnancy (HDP) that identify an increased risk of developing chronic hypertension and cardiovascular disease later in life. Postpartum follow‐up may facilitate early screening and treatment of cardiovascular risk factors. Our objective is to describe patterns of postpartum visits with primary care and women's health providers (eg, family medicine and obstetrics) among women with and without HDP in a nationally representative sample of commercially insured women. Methods and Results We conducted a retrospective cohort study using insurance claims from a US health insurance database to describe patterns in office visits in the 6 months after delivery. We identified 566 059 women with completed pregnancies between 2005 and 2014. At 6 months, 13% of women with normotensive pregnancies, 18% with HDP, and 23% with chronic hypertension had primary care visits (P<0.0001 for comparing HDP and chronic hypertension groups with control participants). Only 58% of women with HDP had 6‐month follow‐up with any continuity provider compared with 47% of women without hypertension (P<0.0001). In multivariable analysis, women with severe preeclampsia were 16% more likely to have postpartum continuity follow‐up (adjusted odds ratio, 1.16; 95% CI, 1.2–1.21). Factors associated with a lower likelihood of any follow‐up included age ≥30 years, Black race, Hispanic ethnicity, and having multiple gestations. Conclusions Rates of continuity care follow‐up after a pregnancy complicated by hypertension were low. This represents a substantial missed opportunity to provide cardiovascular risk screening and management to women at increased risk of future cardiovascular disease.
Effects of herb-partitioned moxibustion for diarrhoea-predominant irritable bowel syndrome

Abstract Background: Diarrhea-predominant irritable bowel syndrome (IBS-D) is a common functional intestinal disease characterized by abdominal pain and diarrhea. Herb-partitioned moxibustion (HPM), a characteristic external therapy, is effective in treating IBS-D. However, no systematic review has been carried out to assess the efficacy and safety of HPM for IBS-D. The aim of this study will systematically evaluate the efficacy and safety of HPM for the treatment of patients with IBS-D. Methods: We will perform the comprehensive literature search in both English and Chinese electronic database including PubMed, Embase, Cochrane Library, Web of Science database, Medline, Chinese BioMedical Literature Database, China National Knowledge Infrastructure, Wanfang database, Chongqing VIP information, and SinoMed from their inception to July 2020. All randomized controlled trials of HPM for the treatment of IBS-D will be included. RevManV5. 3 will be applied to analyze the data. Results: This study will provide high-quality synthesis of current evidence of effectiveness and safety on HPM for patients with IBS-D. Conclusion: The conclusion of our systematic review will provide evidence to judge whether HPM is an effective intervention for IBS-D. Trial registration number: 10.17605/OSF.IO/3JXCZ.
Polarized human cholangiocytes release distinct populations of apical and basolateral small extracellular vesicles

Intercellular communication is critical for organismal homeostasis, and defects can contribute to human disease states. Polarized epithelial cells execute distinct signaling agendas via apical and basolateral surfaces to communicate with different cell types. Small extracellular vesicles (sEVs), including exosomes and small microvesicles, represent an understudied form of intercellular communication in polarized cells. Human cholangiocytes, epithelial cells lining bile ducts, were cultured as polarized epithelia in a Transwell system as a model with which to study polarized sEV communication. Characterization of isolated apically and basolaterally released EVs revealed enrichment in sEVs. However, differences in apical and basolateral sEV composition and numbers were observed. Genetic or pharmacological perturbation of cellular machinery involved in the biogenesis of intralumenal vesicles at endosomes (the source of exosomes) revealed general and domain-specific effects on sEV biogenesis/release. Additionally, analyses of signaling revealed distinct profiles of activation depending on sEV population, target cell, and the function of the endosomal sorting complex required for transport (ESCRT)-associated factor ALG-2–interacting protein X (ALIX) within the donor cells. These results support the conclusion that polarized cholangiocytes release distinct sEV pools to mediate communication via their apical and basolateral domains and suggest that defective ESCRT function may contribute to disease states through altered sEV signaling.
Molecular Dissection of AGPase to Improve Wheat Productivity

The present investigation was conducted towards understanding role of ADP-glucose pyrophosphorylase (AGPase) in developing grains’ starch biosynthesis among diverse wheat lines including disomic chromosome substitution (DCS) lines of C-591 in the background of Chinese Spring, dwarf wheat varieties. isogenic lines and for WH-711 X WH 542. The experiment was laid out in completely randomized block design with three replications. The traits like grain growth rate (GGR) and AGPase enzyme activity were also measured for developing grains at 10, 20 and 30 days after anthesis (DAA). Metabolites like total sugars, reducing sugars, non-reducing sugars, total starch, amylose and amylopectin were also quantified using wheat flour of the test lines. The recorded data was subjected to appropriate statistical analysis to arrive at inferences on the experiments in the study. Critical analysis of the data led us to conclude that determinants for AGPase expression are present across the genome over multiple chromosomes which are active at particular grain filling stages like during first half, chromosome 1B and 2D played major role whereas second half was mainly controlled by chromosome 6A and 5B. Hybridization efforts have improved grain attributes and yield components of WH-542 with ~28.6 % and 42 % enhancement in grain wt. per spike and yield per plant respectively. Maximum amylose (%) was observed in DCS line 2D which can be used to produce functional wheat flour. Grain yield was found to be significantly associated with grains/spike and plant biomass in case of DCS lines. Partitioning the variation by path analysis indicates grains per spike, though had less direct effect but its impact on grain yield through indirect effect via tiller no./plant and AGPase activity at 20 DAA was high suggesting their importance in yield improvement efforts. AGPase activity analysis suggests major contribution of chromosome 2D towards yield in a specific temperature window of 22±1.5C during initial stages of grain filling which can be used as a screening tool for terminal heat tolerant genotypes. MAJOR ADVISOR SIGNATURE OF STUDENT HEAD OF THE DEPARTMENT Title of Thesis : Molecular Dissection of AGPase to Improve Wheat Productivity Full Name of the Degree Holder : RITU BATRA Admission Number : 2010BS44D Title of the Degree : Doctor of Philosophy Name of Discipline : Molecular Biology and Biotechnology Name and Address of Major Advisor : Dr. Virender K. Sikka (Sr. Scientist) Department of Molecular Biology, Biotechnology and Bioinformatics College of Basic Sciences & Humanities CCS HAU, Hisar, Haryana-125 004 Degree Awarding University : CCS Haryana Agricultural University Hisar-125 004 (Haryana), India Year of Award of Degree : 2014 Major Subject : Molecular Biology and Biotechnology Total Number of Pages in Thesis : 68+viii Number of Words in Abstract : 322 Key words : Wheat, DCS , AGPase , GGR, DAA
Optimisation of Source Selection and Design of High Frequency LCL Based Wireless Power Transmission Array

Electric vehicles are exponentially rising in the real world, due to the rise in concern about environmental degradation. Shortly, E-mobility will overtake internal combustion engine mobility. Wireless power transfer (WPT) is a rapidly growing technology, but there is development concerning in development of technology such as high frequency signals, transmission range, size, and efficiency. WPT is a convenient topology for charging Electric Vehicles (EV). A parking lot with fewer charging stations would be inconvenient to charge vehicles. Using WPT there is no need to connect the vehicle to the charging station making it user-friendly and accessible. As the number of vehicles is being connected to the grid to charge there is a great load on the grid. In this paper, the design of a wireless battery charging station with Inductive Wireless Power Transfer (IWPT) topology using renewable energies such as solar energy farm, wind energy farm, and AC grid as sources by choosing the source energy optimally which are subjected to environmental condition for charging EVs in the parking lot with the varying number of vehicles is proposed.
Paternal gene pool of Malays in Southeast Asia and its applications for the early expansion of Austronesians

The origin and differentiation of Austronesian populations and their languages have long fascinated linguists, archeologists, and geneticists. However, the founding process of Austronesians and when they separated from their close relatives, such as the Daic and Austro‐Asiatic populations in the mainland of Asia, remain unclear. In this study, we explored the paternal origin of Malays in Southeast Asia and the early differentiation of Austronesians.
MicroRNAs Modulate the Pathogenesis of Alzheimer’s Disease: An In Silico Analysis in the Human Brain

MicroRNAs (miRNAs) are small RNAs involved in the post-transcriptional regulation of their target genes, causing a decrease in protein translation from the mRNA. Different miRNAs are found in the nervous system, where they are involved in its physiological functions, but altered miRNAs expression was also reported in neurodegenerative disorders, including Alzheimer’s disease (AD). AD is characterized by memory loss, cognitive function abnormalities, and various neuropsychiatric disturbances. AD hallmarks are amyloid β (Aβ) aggregates, called senile plaques, and neurofibrillary tangles (NFTs) formed by hyperphosphorylated Tau protein. In this study, we performed an in silico analysis to evaluate altered patterns of miRNAs expression in the brains of AD patients compared to healthy subjects. We found 12 miRNAs that were differentially expressed in AD compared to healthy individuals. These miRNAs have target genes involved in AD pathogenesis. In particular, some miRNAs influence Aβ production, having as target secretase and amyloid precursor protein (APP). Some miRNAs were reported to be involved in nervous system functions, and their alteration can cause neuronal dysfunction.
The molecular and cellular features of 2-cell-like cells: a reference guide

ABSTRACT Currently, two main cell culture models predominate pluripotent stem cell research: embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Thanks to their ability to contribute to and form all tissues within the body, ESCs and iPSCs have proven invaluable in understanding pluripotent states, early embryonic development and cell differentiation, as well as in devising strategies for regenerative medicine. Comparatively little is known about totipotency – a cellular state with greater developmental potential. In mice, only the zygote and the blastomeres of the 2-cell-stage embryo are truly totipotent, as they alone can develop to form the embryo and all of its supportive extra-embryonic tissues. However, the discovery of a rare subpopulation of cells in murine ESC cultures, possessing features of 2-cell embryo blastomeres and expanded cell fate potential, has provided a biochemically tractable model to enable the in vitro study of totipotency. Here, we summarize current known features of these 2-cell-like cells (2CLCs) in an effort to provide a reference for the community, and to clarify what we know about their identity so far. Summary: This Primer summarizes the current known features of 2-cell-like cells (2CLCs), providing a reference for the community and clarifying what we know about 2CLC identity.
Efficacy of Selpercatinib in RET-Altered Thyroid Cancers.

BACKGROUND RET mutations occur in 70% of medullary thyroid cancers, and RET fusions occur rarely in other thyroid cancers. In patients with RET-altered thyroid cancers, the efficacy and safety of selective RET inhibition are unknown. METHODS We enrolled patients with RET-mutant medullary thyroid cancer with or without previous vandetanib or cabozantinib treatment, as well as those with previously treated RET fusion-positive thyroid cancer, in a phase 1-2 trial of selpercatinib. The primary end point was an objective response (a complete or partial response), as determined by an independent review committee. Secondary end points included the duration of response, progression-free survival, and safety. RESULTS In the first 55 consecutively enrolled patients with RET-mutant medullary thyroid cancer who had previously received vandetanib, cabozantinib, or both, the percentage who had a response was 69% (95% confidence interval [CI], 55 to 81), and 1-year progression-free survival was 82% (95% CI, 69 to 90). In 88 patients with RET-mutant medullary thyroid cancer who had not previously received vandetanib or cabozantinib, the percentage who had a response was 73% (95% CI, 62 to 82), and 1-year progression-free survival was 92% (95% CI, 82 to 97). In 19 patients with previously treated RET fusion-positive thyroid cancer, the percentage who had a response was 79% (95% CI, 54 to 94), and 1-year progression-free survival was 64% (95% CI, 37 to 82). The most common adverse events of grade 3 or higher were hypertension (in 21% of the patients), increased alanine aminotransferase level (in 11%), increased aspartate aminotransferase level (in 9%), hyponatremia (in 8%), and diarrhea (in 6%). Of all 531 patients treated, 12 (2%) discontinued selpercatinib owing to drug-related adverse events. CONCLUSIONS In this phase 1-2 trial, selpercatinib showed durable efficacy with mainly low-grade toxic effects in patients with medullary thyroid cancer with and without previous vandetanib or cabozantinib treatment. (Funded by Loxo Oncology and others; LIBRETTO-001 ClinicalTrials.gov number, NCT03157128.).
Re-evaluation of the criteria for asymmetric amplatzer occluders in the closure of perimembranous ventricular septal defects

Abstract Rationale: To discuss suitable criteria for the application of asymmetric Amplatzer occluders for perimembranous ventricular septal defects (pmVSDs). Patients concerns and diagnoses: We retrospectively studied 18 children with perimembranous VSDs who underwent attempted asymmetric occluder closure between January 2015 and December 2018 in our center. Interventions: Asymmetric Amplatzer occluders were attempted to be placed to all the enrolled patients. We analyzed the diameter of the defects with the receiver operating characteristic curve (ROC) values, the size of the occluders attempted, the presence of aneurysm and the presence of aortic valve prolapse for each patient. Then, for patients who experienced successful device implantation, the therapeutic efficiency was evaluated by follow-up. Outcomes: Only 5 out of a total of 18 patients completed successful device implantation. Compared with failed cases, successful cases demonstrated a significantly smaller VSD size (5.46 ± 1.03 mm vs. 8.73 ± 2.33 mm, P = 0.012) and had a low ratio of aortic valvar prolapse (20% vs. 76.92%, P = 0.026). Four out of 5 successful cases involved arrhythmia complications, but the rhythm of the heart recovered after drug treatment. According to the ROC and Youden analyses, the cut-off value of the defect size for successful asymmetric Amplatzer occluder implantation was no larger than 5.7 mm. Lessons: The application of an asymmetric Amplatzer occluder expands the range of indications for patients with superior localized VSD but is largely limited in cases with aortic valvar prolapse and large VSD sizes. All successful cases recovered from arrhythmia postprocedure.
Microsatellite DNA Analysis of Genetic Diversity in Five Freshwater Mussels in Qinglan Lake, Jiangxi Province

Freshwater mussels play an important role in many aquatic ecosystems, but little is known about their biodiversity and conservation genetics. In order to provide genetic information of freshwater mussels in China, microsatellite markers were used to investigate genetic diversity of five species of freshwater mussels in family Unionidae, Hyriopsis cumingii, Cristaria plicata, Lamprotula caveata, Schistodesmus lampreyanus and Acuticosta chinensis, which were collected from Qinglan Lake, Jiangxi Province. Eight primers for polymorphic DNA microsatellite loci were sieved from 20 primers which were developed from oyster mussel (Epioblasma capsaeformis) (Bivalvia: Unionidae) in North America and freshwater pearl mussel (Margaritifera margaritifera L.) (Bivalvia: Margaritiferidae) in Central Europe. Allelic richness ranged from 2 to 6 alleles per locus and averaged from 2.8750 to 4.000 alleles for five mussel populations. Heterozygosity levels varied from 0.4417 to 0.6333 for average observed heterozygosity (HO ) and 0.4430 to 0.5706 for average expected heterozygosity (HE ), average polymorphism information content (PIC ) ranged from 0.368 to 0.498, which revealed that five populations showed higher genetic diversity. Many loci in different populations deviated from Hardy Weinberg equilibrium. Genetic distance (D) among five mussel populations ranged from 0.6228 to 1.4724, the maximal D was 1.4724 between H. cumingii and C. plicata, and the minimal D was 0.6228 between S. lampreyanus and L. caveata. The study of genetic diversity for freshwater mussels can provide guidelines for resource conservation and genetic improvement.