Miracle-dz/UniFAR-FTUs huggingface dataset:
New Myocyte Formation in the Adult Heart: Endogenous Sources and Therapeutic Implications

Death of adult cardiac myocytes and supportive tissues resulting from cardiovascular diseases such as myocardial infarction is the proximal driver of pathological ventricular remodeling that often culminates in heart failure. Unfortunately, no currently available therapeutic barring heart transplantation can directly replenish myocytes lost from the injured heart. For decades, the field has struggled to define the intrinsic capacity and cellular sources for endogenous myocyte turnover in pursuing more innovative therapeutic strategies aimed at regenerating the injured heart. Although controversy persists to this day as to the best therapeutic regenerative strategy to use, a growing consensus has been reached that the very limited capacity for new myocyte formation in the adult mammalian heart is because of proliferation of existing cardiac myocytes but not because of the activity of an endogenous progenitor cell source of some sort. Hence, future therapeutic approaches should take into consideration the fundamental biology of myocyte renewal in designing strategies to potentially replenish these cells in the injured heart.
Letter by Zheng and Niu Regarding Article, "Cerebrovascular Accidents During Mechanical Circulatory Support: New Predictors of Ischemic and Hemorrhagic Strokes and Outcome".

To the Editor: We read with great interest the article by Izzy et al in a recent issue of Stroke. The results of the study showed that chronic obstructive pulmonary disease increases the risk of ischemic stroke in patients on left ventricular assist devices (LVADs) support, whereas dialysis may increase the risk of hemorrhagic stroke. In addition, the authors concluded that post-LVAD hemorrhagic stroke was associated with higher mortality compared with ischemic stroke. We have several concerns about the study. First, the results of the multivariable logistic regression showed that both post-LVAD ischemic stroke (odds ratio, 3.17; 95% CI, 1.13–8.85; P=0.028) and post-LVAD hemorrhagic stroke (odds ratio, 3.92; 95% CI, 1.34–11.45; P=0.013) were associated with increased mortality. Even though the effect estimates (ie, odds ratios) are somewhat different, the 95% CIs overlap considerably. Hence, on basis of the current analysis, it is not possible to conclude that post-LVAD hemorrhagic stroke was associated with higher mortality compared with post-LVAD ischemic stroke. Second, a recently published study suggested that the risk of post-LVAD stroke and death is bimodal, with the highest risk at the time of implant and increasing risk again after 9 to 12 months. In addition, risk factors for ischemic and hemorrhagic stroke vary in the immediate postoperative period compared with the postdischarge period, which may be because anesthesia and surgery-related stroke risk factors are critical in the immediate postoperative period, whereas antithrombotic status and conventional stroke risk factors are critical in the postdischarge period. If that is the case, the clinical reference value of the conclusions of the study by Izzy et al could be comprised because the lack of the implementation of distinguishing between immediate postoperative period and postdischarge period. Third, it seems that all of the 18 variables were included in the multivariate logistic regression model at the same time when investigating the variables associated with post-LVAD mortality. Including too many variables in the model may lead to a mathematically unstable outcome with decreased generalizability beyond the current study sample and lead to large SEs with wide and imprecise CIs. On the other hand, if included variables are highly correlated with one another (known as multicollinearity), the effect of each variable on the regression model becomes less precise. Table 2 in the study by Izzy et al showed that many variables were independently associated with stroke. For example, dialysis was highly associated with post-LVAD hemorrhagic stroke with a high hazard ratio (hazard ratio, 9.57; P=0.005). Thus, we recommend the authors using proper statistical methods such as inputting variables potentially related to mortality (eg, P≤0.10) into a multivariable logistic regression model and using a certain selection method (eg, stepwise backward selection).
Agricultural land use among mestizo colonist and indigenous populations: Contrasting patterns in the Amazon

This paper compares land use patterns of mestizo colonists and indigenous populations in the central Ecuadorian Amazon, based on data from a household survey covering mestizo colonist, Kichwa and Shuar households. As expected, colonists mostly engage in commercial agriculture and cattle ranching, but there are substantial differences in land use patterns between the Shuar and the Kichwa. The Shuar engage in cash cropping and cattle ranching, and on average, devote even more land to agricultural uses than mestizo colonists in this sample. In contrast, the Kichwa engage more in subsistence crop production and less in commercial agriculture. Such different patterns appear related to local conditions, earlier migratory and settlement patterns, and the level of exposure to markets. The implications of this for policy are explored in the conclusions.
Epidemiology of Upper Extremity Injuries in NCAA Men’s and Women’s Ice Hockey

Background: Ice hockey is a physically demanding sport where athletes are susceptible to a variety of injuries. Several studies reported the overall injury rates in ice hockey; however, there is a paucity of information on upper extremity (UE) injuries among collegiate ice hockey players. Purpose: To describe the epidemiology of UE injuries among collegiate male and female ice hockey players with NCAA (National Collegiate Athletic Association) injury surveillance data from 2004-2005 to 2013-2014. Study Design: Descriptive epidemiology study. Methods: Data were obtained from the NCAA Injury Surveillance Program for all UE injuries sustained during the academic years 2004-2005 to 2013-2014. Injury rates, rate ratios (RRs), and injury proportion ratios were reported with 95% CIs. Results: During the 10 years studied, the overall rate of UE injuries for men was higher than that for women (236 vs 125 injuries per 100,000 athlete-exposures [AEs]; RR, 1.89; 95% CI, 1.67-2.15). UE injuries sustained during either pre- or postseason were approximately 3 times higher for men than for women (preseason: 149 vs 53 per 100,000 AEs; RR, 2.83; 95% CI, 1.69-4.74; postseason: 143 vs 49 per 100,000 AEs; RR, 2.91; 95% CI, 1.33-6.38). The overall injury rate was highest during the regular season (men: 257 per 100,000 AEs; 95% CI, 242-272; women: 143 per 100,000 AEs; 95% CI, 126-160). Additionally, the injury rate for men and women was higher during competition than practice (men: 733 vs 83 per 100,000 AEs; 95% CI, 687-780 and 75-92; women: 303 vs 64 per 100,000 AEs; 95% CI, 259-348 and 52-76). The most common injury observed was acromioclavicular joint sprain (men, 29.1%; women, 13.8%). For both groups, acromioclavicular joint injuries accounted for most non–time loss, moderate time loss (2-13 days), and severe time loss (≥14 days) injuries. Conclusion: Men and women sustained a significant number of UE injuries playing collegiate ice hockey during the period studied, with acromioclavicular joint sprain being the most common UE injury and the one that most frequently led to significant time loss. These data may provide insight for future injury prevention and guide improvements in training.
The Influence of Body Size on the Expression of Sexually Dimorphic Morphological Traits

Skeletal sexual dimorphism manifests as size or shape differences between males and females in a population. Certain dimorphic traits are used in sex estimation methods, and populational variation in the expression of these traits can result in inaccurate sex estimation. However, the underlying causes of variation in trait expression remain unclear. This study explores body size, which also exhibits sexual dimorphism, as a potential factor influencing trait expression. To test this, skeletons of 209 individuals of varying body size were analyzed, and morphological traits were scored according to the Walker (2008), Klales et al. (2012), and Rogers (1999) sex estimation methods. Statistical analyses found significant correlations between body size parameters and expression of traits, with stature explaining more relative variance in trait expression than body mass. However, the relationships are weak and few in number, suggesting that body size has a minimal impact on the expression of these morphological traits.
Structural basis of VHH-mediated neutralization of the food-borne pathogen Listeria monocytogenes

Listeria monocytogenes causes listeriosis, a potentially fatal food-borne disease. The condition is especially harmful to pregnant women. Listeria outbreaks can originate from diverse foods, highlighting the need for novel strategies to improve food safety. The first step in Listeria invasion is internalization of the bacteria, which is mediated by the interaction of the internalin family of virulence factors with host cell receptors. A crucial interaction for Listeria invasion of the placenta, and thus a target for therapeutic intervention, is between internalin B (InlB) and the receptor c-Met. Single-domain antibodies (VHH, also called nanobodies, or sdAbs) from camel heavy-chain antibodies are a novel solution for preventing Listeria infections. The VHH R303, R330, and R326 all bind InlB with high affinity; however, the molecular mechanism behind their mode of action was unknown. We demonstrate that despite a high degree of sequence and structural diversity, the VHH bind a single epitope on InlB. A combination of gentamicin protection assays and florescent microscopy establish that InlB-specific VHH inhibit Listeria invasion of HeLa cells. A high-resolution X-ray structure of VHH R303 in complex with InlB showed that the VHH binds at the c-Met interaction site on InlB, thereby acting as a competitive inhibitor preventing bacterial invasion. These results point to the potential of VHH as a novel class of therapeutics for the prevention of listeriosis.
MODELING AND SIMULATION OF FIELD ORIENTED CONTROL INDUCTION MOTOR DRIVE AND INFLUENCE OF ROTOR RESISTANCE VARIATIONS ON ITS PERFORMANCE

Induction motor with rotor flux based indirect field oriented control is well suited for high performance applications due to its excellent dynamic behavior. The overriding feature of this control method is its ease of implementation and linearity of the torque versus slip characteristics. But, the indirect field oriented controller is sensitive to variations in motor parameters, especially variation in rotor time constant. This paper presents the modeling and analysis of a voltage controlled rotor flux based indirect field oriented control induction motor motion control system. with detailed analysis of controller design in discrete system. The influence of rotor resistance variation on the performance of drive like effect on speed, rotor flux and electromagnetic torque under different operating is also studied.
Controlling the 3D Electromagnetic Coupling in Co-Sputtered Ag–SiO2 Nanomace Arrays by Lateral Sizes

Ag–SiO2 nanomace arrays were prepared on a two-dimensional ordered colloidal (2D) polystyrene sphere template by co-sputtering Ag and SiO2 in a magnetron sputtering system. The lateral size of the nanomaces and the distance between the neighbor nanomaces were controlled by adjusting the etching time of the 2D template. The nanomaces were composed of SiO2-isolated Ag nanoparticles, which produced surface-enhanced Raman scattering (SERS) enhancement, and 3D hot spots were created between the neighbor nanomaces. When the distance between the nanomaces was sufficiently large, triangle-shaped nanostructures on silicon substrate were observed, which also contributed to the enhancement of the SERS signals. The finite-difference time-domain (FDTD) method was used to calculate the electromagnetic field distributions in the Ag–SiO2 nanomace arrays, which generated physical reasons for the change of the SERS signals.
Symbiotic organisms search algorithm for the unrelated parallel machines scheduling with sequence-dependent setup times

This paper addresses the problem of makespan minimization on unrelated parallel machines with sequence dependent setup times. The symbiotic organisms search (SOS) algorithm is a new and popular global optimization technique that has received wide acceptance in recent years from researchers in continuous and discrete optimization domains. An improved SOS algorithm is developed to solve the parallel machine scheduling problem. Since the standard SOS algorithm was originally developed to solve continuous optimization problems, a new solution representation and decoding procedure is designed to make the SOS algorithm suitable for the unrelated parallel machine scheduling problem (UPMSP). Similarly, to enhance the solution quality of the SOS algorithm, an iterated local search strategy based on combining variable numbers of insertion and swap moves is incorporated into the SOS algorithm. More so, to further improve the SOS optimization speed and performance, the longest processing time first (LPT) rule is used to design a machine assignment heuristic that assigns processing machines to jobs based on the machine dynamic load-balancing mechanism. Subsequently, the machine assignment scheme is incorporated into SOS algorithms and used to solve the UPMSP. The performances of the proposed methods are evaluated by comparing their solutions with other existing techniques from the literature. A number of statistical tests were also conducted to determine the variations in performance for each of the techniques. The experimental results showed that the SOS with LPT (SOS-LPT) heuristic has the best performance compared to other tested method, which is closely followed by SOS algorithm, indicating that the two proposed algorithms’ solution approaches are reasonable and effective for solving large-scale UPMSPs.
A Word to the Wise: Adolescent Reactions to Parental Communication about Weight.

BACKGROUND With high rates of adolescent obesity, many parents are talking to adolescents about their body weight. Parental "weight talk" is linked with adverse health behaviors in youth, but we know little about what parents say in these conversations. Using a weight loss treatment-seeking sample of adolescents, the present study assessed adolescents' emotional reactions to words their parents use to describe their weight and preferred language for these interactions. METHODS Adolescents enrolled in a national weight loss camp (N = 148) completed a questionnaire assessing their preferences for, and emotional reactions to, parental use of 18 words to describe their body weight. RESULTS Findings showed a diverse range of weight language used by parents, with many words inducing negative emotional reactions of embarrassment, shame, and sadness in adolescents. Emotional responses to weight language varied according to adolescents' gender, BMI, and experience of weight-teasing from family members. CONCLUSIONS Findings underscore the need to carefully consider language; parents should avoid making assumptions about what language to use in conversations with adolescents about their weight. Educating parents how to identify negative weight talk may help promote more supportive parental communication about weight-related health.
Goniotomy Using the Kahook Dual Blade in Severe and Refractory Glaucoma: 6-Month Outcomes

Purpose: The purpose of the study is to describe short-term efficacy and safety of goniotomy with trabecular meshwork excision using the Kahook Dual Blade (KDB, New World Medical Inc., Rancho Cucamonga, CA) in patients with severe or refractory glaucoma. Method: Retrospective multicentric case series of 53 eyes with severe or refractory glaucoma as defined by ICD-10 conducted in the United States, Mexico, and Switzerland. Primary efficacy outcome was a ≥20% decrease in intraocular pressure (IOP) from baseline at 6 months. Secondary efficacy outcome measures were probability of achieving an IOP⩽14 or 18 mm Hg at 6 months and the mean IOP change from baseline at 6 months. Medication use required to obtain target IOP at last follow up and adverse effects were analyzed. Results: The proportion of eyes achieving an IOP reduction of >20% from preoperative baseline at 6 months was 57.7% (n=30). The mean IOP decreased from 18.4±6.1 mm Hg at baseline to 13.9±3.5 mm Hg at month 6 (23.9% reduction; P<0.001). At 6 months, 63.5% and 92.3% achieved an IOP⩽14 and ⩽18 mm Hg, respectively, and the mean number of glaucoma medications was reduced by 1.2±1.3 (36.6%) compared with baseline (P<0.001). The most common adverse event was hyphema (n=29, 34.9%) with spontaneous resolution in all cases. No severe complications were reported. One case presented with uncontrolled IOP and required glaucoma drainage device surgery at 1 month. Conclusions: Goniotomy with trabecular meshwork excision using the KDB could be an alternative surgery for severe or refractory glaucoma, significantly reducing IOP and medication use at 6 months, with a low rate of complications.
Para-probiotics for Preterm Neonates—The Next Frontier

Current evidence supports the use of probiotics in preterm neonates for prevention of necrotizing enterocolitis, mortality and late onset sepsis. Despite the strong evidence, the uptake of this intervention has not been universal due to concerns including probiotic sepsis, pro-inflammatory response and transmission of antibiotic resistance. Critically ill extremely preterm neonates with potentially compromised gut integrity are at higher risk of probiotic sepsis due to translocation. In most countries, probiotics are sold as food supplements with poor quality control. The traditional definition of probiotics as “live microorganisms” has been challenged as many experts have questioned the importance of viability in the context of the beneficial effects of probiotics. Paraprobiotics (ghost probiotics), are defined as non-viable microbial cells (intact or broken) or crude cell extracts (i.e., with complex chemical composition), which, when administered (orally or topically) in adequate amounts, confer a benefit on the human or animal consumer. Current evidence indicates that paraprobiotics could be safe alternatives to probiotics in preterm neonates. High-quality pre-clinical and clinical studies including adequately powered randomised controlled trials (RCTs) are warranted in preterm neonates to explore this new frontier.
Proteolytic ectodomain shedding of membrane proteins in mammals—hardware, concepts, and recent developments

Proteolytic removal of membrane protein ectodomains (ectodomain shedding) is a post‐translational modification that controls levels and function of hundreds of membrane proteins. The contributing proteases, referred to as sheddases, act as important molecular switches in processes ranging from signaling to cell adhesion. When deregulated, ectodomain shedding is linked to pathologies such as inflammation and Alzheimer's disease. While proteases of the “a disintegrin and metalloprotease” (ADAM) and “beta‐site APP cleaving enzyme” (BACE) families are widely considered as sheddases, in recent years a much broader range of proteases, including intramembrane and soluble proteases, were shown to catalyze similar cleavage reactions. This review demonstrates that shedding is a fundamental process in cell biology and discusses the current understanding of sheddases and their substrates, molecular mechanisms and cellular localizations, as well as physiological functions of protein ectodomain shedding. Moreover, we provide an operational definition of shedding and highlight recent conceptual advances in the field. While new developments in proteomics facilitate substrate discovery, we expect that shedding is not a rare exception, but rather the rule for many membrane proteins, and that many more interesting shedding functions await discovery.
rbpms2 functions in Balbiani body architecture and ovary fate

The most prominent developmental regulators in oocytes are RNA-binding proteins (RNAbps) that assemble their targets into ribonucleoprotein granules where they are stored, transported and translationally regulated. RNA-binding protein of multiple splice forms 2, or Rbpms2, interacts with molecules that are essential to reproduction and egg patterning, including bucky ball, a key factor for Bb formation. Rbpms2 is localized to germ granules in primordial germ cells (PGCs) and to the Balbiani body (Bb) of oocytes, although the mechanisms regulating Rbpms2 localization to these structures are unknown. Using mutant Rbpms2 proteins, we show that Rbpms2 requires distinct protein domains to localize within germ cells and somatic cells. Accumulation and localization to subcellular compartments in the germline requires an intact RNA binding domain. Whereas in zebrafish somatic blastula cells, the conserved C-terminal domain promotes localization to the bipolar centrosomes/spindle. To investigate Rbpms2 functions, we mutated the duplicated and functionally redundant zebrafish rbpms2 genes. The gonads of rbpms2a;2b (rbpms2) mutants initially contain early oocytes, however definitive oogenesis ultimately fails during sexual differentiation and, rbpms2 mutants develop as fertile males. Unlike other genes that promote oogenesis, failure to maintain oocytes in rbpms2 mutants was not suppressed by mutation of Tp53. These findings reveal a novel and essential role for rbpms2 in oogenesis. Ultrastructural and immunohistochemical analyses revealed that rbpms2 is not required for the asymmetric accumulation of mitochondria and Buc protein in oocytes, however its absence resulted in formation of abnormal Buc aggregates and atypical electron-dense cytoplasmic inclusions. Our findings reveal novel and essential roles for rbpms2 in Buc organization and oocyte differentiation.
Current and emerging treatment options for endometriosis

ABSTRACT Introduction: Pharmacotherapy has a pivotal role in the management of endometriosis with long-term treatments balancing clinical efficacy (control of pain symptoms and prevention of recurrence of the disease after surgery) with an acceptable safety profile. Treatment choice is based on several factors including age and patient preference, reproductive plans, intensity of pain, severity of disease and incidence of adverse effects. Areas covered: The aim of this review is to provide the reader with a complete overview of drugs that are currently available or are under investigation for the treatment of endometriosis highlighting on-going clinical trials. Expert opinion: Almost all of the available treatment options for endometriosis suppress ovarian function and are not curative. Combined oral contraceptives and progestins are commonly administered to these patients in order to ameliorate pain symptoms. Gonadotropin-releasing hormone-agonists are prescribed when first-line therapies are ineffective, not tolerated or contraindicated. Aromatase inhibitors should be reserved only for women who are refractory to other treatments. Amongst the drugs under development, gonadotropin-releasing hormone antagonists have shown the most promising results. Presently, are a number of potential therapies currently in pre-clinical or early clinical studies which may alter treatment strategies in the future although further studies are necessary.