Miracle-dz/UniFAR-FTUs huggingface dataset:
Electrical Transport Ability of Nanostructured Potassium-Doped Titanium Oxide Film

Potassium-doped nanostructured titanium oxide films were fabricated using a wet corrosion process with various KOH solutions. The doped condition of potassium in TiO2 was confirmed by Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). Nanotubular were synthesized at a dopant concentration of <0.27%; when the dopant concentration increased to >0.27%, these structures disappeared. To investigate the electrical properties of K-doped TiO2, pseudo metal–oxide–semiconductor field-effect transistor (MOSFET) samples were fabricated. The samples exhibited a distinct electrical behavior and p-type characteristics. The electrical behavior was governed by the volume of the dopant when the dopant concentration was <0.10% and the volume of the TiO2 phase when the dopant concentration was >0.18%.
Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF-7 cell line

Ampelopsis megalophylla has been found to demonstrate anticancer activities in human cancer cells; however, the effect of total flavone extract (TFE), commonly used in Traditional Chinese Medicine, remains unclear. Furthermore, there is limited information on its effects on breast cancer cell lines. The present study aimed to investigate the inhibitory effects of TFE in different human cancer cell lines. In addition, the underlying mechanisms and the signaling pathways involved were also investigated by determining tumor cell morphological changes, and differences in the cell cycle, apoptosis, mitochondrial transmembrane potential, and related protein expression levels in a breast cancer cell line. It was found that TFE inhibited proliferation in seven cancer cell lines (HeLa, A549, MCF-7, HepG2, A2780, SW620 and MDA-MB-231 and demonstrated a strong inhibitory effect on MCF-7 cell proliferation. Cell morphological changes were also observed and arrested at the G2/M phase following treatment with TFE at different concentrations. In addition, TFE disrupted the mitochondrial membrane potential and upregulated the expression level of apoptotic proteins, including caspase-3, -8 and -9, the Bax/Bcl-2 ratio, and Apaf-1 in time-dependent manner. These results indicated that TFE induced apoptosis of the MCF-7 cells via a mitochondrial-mediated apoptotic pathway. In conclusion, TFE is potentially effective in treating breast cancer.
A Narrative Review of Limb Dominance: Task Specificity and the Importance of Fitness Testing.

ABSTRACT Virgile, A and Bishop, C. A narrative review of limb dominance: Task specificity and the importance of fitness testing. J Strength Cond Res XX(X): 000-000, 2020-Preferential limb function must be sustained through repetitious asymmetrical activities for continuous athletic development and, ultimately, optimal athletic performance. As such, the prevalence of limb dominance and between-limb differences is common in athletes. Severe between-limb differences have been associated with reductions in athletic performance and increased injury risk in athletes. However, in the current literature, the terms limb preference and limb dominance have been used interchangeably. Together, these terms include a limb that is subjectively preferred and one that is objectively dominant in 1 or more performance measures from a variety of athletic tasks. In this review, we (a) discuss reported correspondence between task-specific limb preference and limb dominance outcomes in athletes, (b) provide greater context and distinction between the terms limb preference and limb dominance, and (c) offer pragmatic strategies for practitioners to assess context-specific limb dominance. A limb that is subjectively preferred is not necessarily objectively dominant in 1 or more athletic qualities or sport-specific tasks. Further to this, a limb that is objectively superior in 1 task may not exhibit such superiority in a separate task. Thus, limb preference and limb dominance are both task-specific. As such, we propose that practitioners intentionally select tasks for limb dominance assessment which resemble the most relevant demands of sport. Because limb dominance profiles are inconsistent, we suggest that practitioners increase assessment frequency by integrating limb dominance testing into standard training activities. This will allow practitioners to better understand when changes reflect sport-specific adaptation vs. potential performance or injury ramifications.
Synthesis of polybenzimidazoles with sulfonic acid groups

2-Sulfoterephthalic acid (STA) and disulfoisophthalic acid (DSIA) were synthesized through the sulfonation and the oxidation of m- and p-xylene. The polycondensation reactions of STA and DSIA with aromatic tetraamines gave polybenzimidazoles which contained one or two sulfonic acid groups for each repeating unit. The polymer obtained was soluble in sulfuric acid, some organic solvents, and aqueous strong alkaline solution. The polymers were stable up to 400°C, but they gave polybenzimidazoles above 400°C by eliminating sulfonic acid groups, instead of ring closure.
Mycosporine-Like Amino Acids (MAAs): Biology, Chemistry and Identification Features

Mycosporines and mycosporine-like amino acids are ultra-violet-absorbing compounds produced by several organisms such as lichens, fungi, algae and cyanobacteria, especially upon exposure to solar ultraviolet radiation. These compounds have photoprotective and antioxidant functions. Mycosporine-like amino acids have been used as a natural bioactive ingredient in cosmetic products. Several reviews have already been developed on these photoprotective compounds, but they focus on specific features. Herein, an extremely complete database on mycosporines and mycosporine-like amino acids, covering the whole class of these natural sunscreen compounds known to date, is presented. Currently, this database has 74 compounds and provides information about the chemistry, absorption maxima, protonated mass, fragments and molecular structure of these UV-absorbing compounds as well as their presence in organisms. This platform completes the previous reviews and is available online for free and in the public domain. This database is a useful tool for natural product data mining, dereplication studies, research working in the field of UV-absorbing compounds mycosporines and being integrated in mass spectrometry library software.
Neonicotinoid Insecticides from a Marine Perspective: Acute and Chronic Copepod Testing and Derivation of Environmental Quality Standards

Neonicotinoid insecticides have become of global concern for the aquatic environment. Harpacticoid copepods are among the organisms most sensitive to neonicotinoids. We exposed the brackish copepod Nitocra spinipes to 4 neonicotinoid insecticides (clothianidin, imidacloprid, thiacloprid, and thiamethoxam) to investigate acute toxicity on adults (96‐h exposure) and effects on larval development (7‐d exposure). We used these results in combination with publicly available ecotoxicity data to derive environmental quality standards (EQS). These EQS were ultimately used in a single‐substance and mixture risk assessment for the Belgian part of the North Sea. Acute toxicity testing revealed that immobilization is a more sensitive endpoint than mortality, with 96‐h median effect concentration (EC50) values of 6.9, 7.2, 25, and 120 µg L–1 for clothianidin, thiacloprid, imidacloprid, and thiamethoxam, respectively. In addition, the larval development tests resulted in 7‐d no‐observed‐effect concentrations (NOECs) of 2.5, 2.7, 4.2, and >99 µg L–1 for clothianidin, thiacloprid, imidacloprid, and thiamethoxam, respectively. The derived saltwater annual average (AA‐)EQS were 0.05, 0.0048, 0.002, and 0.016 µg L–1 for clothianidin, thiacloprid, imidacloprid, and thiamethoxam, respectively. Finally, the risk characterization revealed some exceedances of the AA‐EQS in Belgian harbors for imidacloprid (number of exceedances, n = 2/4), for thiacloprid (n = 1/4), for thiamethoxam (n = 1/4), and for the mixture of the 4 neonicotinoids (n = 4/4), but not at the open sea. At the open sea site, the toxic unit sums relative to the AA‐EQS were 0.72 and 0.22, suggesting no mixture risk, albeit with a relatively small margin of safety. Including short‐term EC10 (96‐h) values of N. spinipes for the AA‐EQS derivation led to a refinement of the AA‐EQS for clothianidin and thiamethoxam, suggesting their use for the AA‐EQS derivation because one of the overarching goals of the definition of EQS is to protect species at the population level. Environ Toxicol Chem 2021;40:1353–1367. © 2021 SETAC
Association of Lipid, Inflammatory, and Metabolic Biomarkers With Age at Onset for Incident Coronary Heart Disease in Women.

Importance Risk profiles for premature coronary heart disease (CHD) are unclear. Objective To examine baseline risk profiles for incident CHD in women by age at onset. Design, Setting, and Participants A prospective cohort of US female health professionals participating in the Women's Health Study was conducted; median follow-up was 21.4 years. Participants included 28 024 women aged 45 years or older without known cardiovascular disease. Baseline profiles were obtained from April 30, 1993, to January 24, 1996, and analyses were conducted from October 1, 2017, to October 1, 2020. Exposures More than 50 clinical, lipid, inflammatory, and metabolic risk factors and biomarkers. Main Outcomes and Measures Four age groups were examined (<55, 55 to <65, 65 to <75, and ≥75 years) for CHD onset, and adjusted hazard ratios (aHRs) were calculated using stratified Cox proportional hazard regression models with age as the time scale and adjusting for clinical factors. Women contributed to different age groups over time. Results Of the clinical factors in the women, diabetes had the highest aHR for CHD onset at any age, ranging from 10.71 (95% CI, 5.57-20.60) at CHD onset in those younger than 55 years to 3.47 (95% CI, 2.47-4.87) at CHD onset in those 75 years or older. Risks that were also noted for CHD onset in participants younger than 55 years included metabolic syndrome (aHR, 6.09; 95% CI, 3.60-10.29), hypertension (aHR, 4.58; 95% CI, 2.76-7.60), obesity (aHR, 4.33; 95% CI, 2.31-8.11), and smoking (aHR, 3.92; 95% CI, 2.32-6.63). Myocardial infarction in a parent before age 60 years was associated with 1.5- to 2-fold risk of CHD in participants up to age 75 years. From approximately 50 biomarkers, lipoprotein insulin resistance had the highest standardized aHR: 6.40 (95% CI, 3.14-13.06) for CHD onset in women younger than 55 years, attenuating with age. In comparison, weaker but significant associations with CHD in women younger than 55 years were noted (per SD increment) for low-density lipoprotein cholesterol (aHR, 1.38; 95% CI, 1.10-1.74), non-high-density lipoprotein cholesterol (aHR, 1.67; 95% CI, 1.36-2.04), apolipoprotein B (aHR, 1.89; 95% CI, 1.52-2.35), triglycerides (aHR, 2.14; 95% CI, 1.72-2.67), and inflammatory biomarkers (1.2- to 1.8-fold)-all attenuating with age. Some biomarkers had similar CHD age associations (eg, physical inactivity, lipoprotein[a], total high-density lipoprotein particles), while a few had no association with CHD onset at any age. Most risk factors and biomarkers had associations that attenuated with increasing age at onset. Conclusions and Relevance In this cohort study, diabetes and insulin resistance, in addition to hypertension, obesity, and smoking, appeared to be the strongest risk factors for premature onset of CHD. Most risk factors had attenuated relative rates at older ages.
Soluble and Exosome-Bound α-Galactosylceramide Mediate Preferential Proliferation of Educated NK Cells with Increased Anti-Tumor Capacity

Simple Summary Many cancers down-regulate the expression of major histocompatibility complex class I (MHC-I) to avoid recognition and elimination by the adaptive immune system. Natural Killer (NK) cells, as part of innate immunity, complement tumor recognition by their ability to sense foreign and malignant cells by the lack of major MHC-I expression. During development, NK cells learn to perceive physiological levels of self MHC-I as healthy in a process termed NK cell education. In the current study, we assessed whether the stimulation of natural killer T (NKT) cells could impact the tumor-killing ability of NK cells. We can show that stimulation with a well-established activator of NKT cells induces the preferential expansion of educated NK cells, and improves NK-cell-mediated tumor-killing of both MHC-I+ and MHC-I− tumor cells. Furthermore, this increased tumor-killing capacity of NK cells can be achieved by nanovesicle-mediated delivery, which is known to improve adaptive immunity as well. Our approach thus holds great potential for future cancer immunotherapy. Abstract Natural killer (NK) cells can kill target cells via the recognition of stress molecules and down-regulation of major histocompatibility complex class I (MHC-I). Some NK cells are educated to recognize and kill cells that have lost their MHC-I expression, e.g., tumor or virus-infected cells. A desired property of cancer immunotherapy is, therefore, to activate educated NK cells during anti-tumor responses in vivo. We here analyze NK cell responses to α-galactosylceramide (αGC), a potent activator of invariant NKT (iNKT) cells, or to exosomes loaded with αGC. In mouse strains which express different MHC-I alleles using an extended NK cell flow cytometry panel, we show that αGC induces a biased NK cell proliferation of educated NK cells. Importantly, iNKT cell-induced activation of NK cells selectively increased in vivo missing self-responses, leading to more effective rejection of tumor cells. Exosomes from antigen-presenting cells are attractive anti-cancer therapy tools as they may induce both innate and adaptive immune responses, thereby addressing the hurdle of tumor heterogeneity. Adding αGC to antigen-loaded dendritic-cell-derived exosomes also led to an increase in missing self-responses in addition to boosted T and B cell responses. This study manifests αGC as an attractive adjuvant in cancer immunotherapy, as it increases the functional capacity of educated NK cells and enhances the innate, missing self-based antitumor response.
Crosstalk between Depression and Dementia with Resting-State fMRI Studies and Its Relationship with Cognitive Functioning

Alzheimer’s disease (AD) is the most common type of dementia, and depression is a risk factor for developing AD. Epidemiological studies provide a clinical correlation between late-life depression (LLD) and AD. Depression patients generally remit with no residual symptoms, but LLD patients demonstrate residual cognitive impairment. Due to the lack of effective treatments, understanding how risk factors affect the course of AD is essential to manage AD. Advances in neuroimaging, including resting-state functional MRI (fMRI), have been used to address neural systems that contribute to clinical symptoms and functional changes across various psychiatric disorders. Resting-state fMRI studies have contributed to understanding each of the two diseases, but the link between LLD and AD has not been fully elucidated. This review focuses on three crucial and well-established networks in AD and LLD and discusses the impacts on cognitive decline, clinical symptoms, and prognosis. Three networks are the (1) default mode network, (2) executive control network, and (3) salience network. The multiple properties emphasized here, relevant for the hypothesis of the linkage between LLD and AD, will be further developed by ongoing future studies.
Curcumin/d-mannitol as photolarvicide: induced delay in larval development time, changes in sex ratio and reduced longevity of Aedes aegypti.

BACKGROUND Resistant populations of Ae. aegypti have been a major problem in arboviruses epidemic areas, generating a strong demand for novel methods of vector control. In this regard, our group has demonstrated the use of curcumin as an efficient photoactive larvicide to eliminate Ae. aegypti larvae. This work was aimed to evaluate the Ae. aegypti (Rockefeller) development under sublethal conditions, using a curcumin/d-mannitol (DMC) formulation. The photolarvicidal efficacy under semi-field and field conditions (wild populations) was also analyzed, as well as the photobleaching and residual activity of DMC. RESULTS A delay in development time when larvae were exposed to sublethal concentrations of DMC was observed, followed by significant changes in sex ratio and reduction in longevity. DMC also presented a low residual activity when compared to usual larvicides, and had a substantial photolarvicidal activity against wild populations in field trials, achieving 71.3% mortality after 48 h. CONCLUSIONS Overall, these findings are of great biological importance for the process of enabling the implementation of DMC as a new product in the control of Ae. aegypti larvae, and contributes to the improvement of new plant-based larvicides. This article is protected by copyright. All rights reserved.
A First Look at the Essential Genes of Pseudomonas protegens

Transposon insertion sequencing is a useful tool to identify the genes that are essential for a bacterial species to grow and divide effectively. In this issue of Journal of Bacteriology, Fabian et al. present the first set of transposon insertion sequencing data highlighting the genes essential to the plant-commensal species Pseudomonas protegens strain Pf-5 and describe comparative analyses with other pseudomonads (B. K. Fabian, C. Foster, A. J. Asher, L. D. Elbourne, et al., J Bacteriol 203:e00432-20, 2021, https://doi.org/10.1128/JB.00432-20). ABSTRACT Transposon insertion sequencing is a useful tool to identify the genes that are essential for a bacterial species to grow and divide effectively. In this issue of Journal of Bacteriology, Fabian et al. present the first set of transposon insertion sequencing data highlighting the genes essential to the plant-commensal species Pseudomonas protegens strain Pf-5 and describe comparative analyses with other pseudomonads (B. K. Fabian, C. Foster, A. J. Asher, L. D. Elbourne, et al., J Bacteriol 203:e00432-20, 2021, https://doi.org/10.1128/JB.00432-20).
Adoption of the Anti-Fibrotic Medications Pirfenidone and Nintedanib for Patients with Idiopathic Pulmonary Fibrosis.

RATIONALE In October 2014, the anti-fibrotic medications pirfenidone and nintedanib became the first medications approved by the Food and Drug Administration for use in patients with idiopathic pulmonary fibrosis. Since approval, there has been no non-registry analysis of the real-world adoption of these medications in every day clinical practice. OBJECTIVE To evaluate the adoption, persistence, and out-of-pocket costs of pirfenidone and nintedanib since their approval in the US in 2014 Methods: A retrospective cohort analysis was performed by identifying privately insured and Medicare Advantage beneficiaries with idiopathic pulmonary fibrosis. We then split the patients into three cohorts: those who were untreated and those that filled a prescription for either pirfenidone or nintedanib between October 1, 2014 and July 31, 2019. The primary outcome was adoption of the medications. Secondary outcomes included medication persistence and prescription drug costs. RESULTS A total of 10,996 patients with IPF were identified in the dataset. A minority of patients (26.4%) with idiopathic pulmonary fibrosis identified in the cohort started either medication since approval in 2014, with the adoption of both medications comparable at around 13.2%. Those receiving the medications were younger (72 years old for both medications compared to 73.9, p-value<0.0001) and healthier (3.9 co-morbidities versus 4.9, p-value <0.0001) compared to those not receiving treatment. Men were significantly more likely to receive treatment than woman (30.0% versus 21.9%, p-value <0.0001). 42.8% of treated patients discontinued the medications during the study period. Patients' out-of-pocket expenses per month were high for both drugs (mean, $397.51 for nintedanib; $394.49 for pirfenidone). CONCLUSION The adoption of both the anti-fibrotic medications in the United States in every day practice has been low since approval and may be associated with the high out-of-pocket cost.
Herbal therapy as a promising approach for regulation on lipid profiles: A review of molecular aspects

Impaired lipid profile is defined as abnormal plasma levels of low‐density lipoprotein, triglycerides, and total cholesterol. This disease state is associated with the development and progression of various disorders, such as diabetes mellitus, cardiovascular diseases, and acute myocardial infarction. Globally, all of these disorders are related to a significant rate of death. Therefore, finding a suitable approach for the prevention and treatment of lipid profile‐related disorders is in the spotlight. Recently, herbal therapy has been considered a promising therapeutic approach for the treatment of hyperlipidemia or its related disorders due to its safety and efficacy. Hereby, we address the potential benefits of some of these herbal compounds on different aspects of lipid profile and its abnormalities with a special focus on their underlying mechanisms. Using herbal products, such as teas and mushrooms, or their derivatives, Rosmarinus officinalis Linn, Curcuma longa, Green tea, Lippia triphylla, Lippia citriodora, Plantago asiatica L, Vine tea, and Grifola frondosa have been proved to exert several therapeutic impacts on lipid profile and its related disorders, and we would provide a brief review on them in this literature.
Therapeutic Properties of Mesenchymal Stromal/Stem Cells: The Need of Cell Priming for Cell-Free Therapies in Regenerative Medicine

Mesenchymal stromal/stem cells (MSCs) are multipotent adult stem cells that support homeostasis during tissue regeneration. In the last decade, cell therapies based on the use of MSCs have emerged as a promising strategy in the field of regenerative medicine. Although these cells possess robust therapeutic properties that can be applied in the treatment of different diseases, variables in preclinical and clinical trials lead to inconsistent outcomes. MSC therapeutic effects result from the secretion of bioactive molecules affected by either local microenvironment or MSC culture conditions. Hence, MSC paracrine action is currently being explored in several clinical settings either using a conditioned medium (CM) or MSC-derived exosomes (EXOs), where these products modulate tissue responses in different types of injuries. In this scenario, MSC paracrine mechanisms provide a promising framework for enhancing MSC therapeutic benefits, where the composition of secretome can be modulated by priming of the MSCs. In this review, we examine the literature on the priming of MSCs as a tool to enhance their therapeutic properties applicable to the main processes involved in tissue regeneration, including the reduction of fibrosis, the immunomodulation, the stimulation of angiogenesis, and the stimulation of resident progenitor cells, thereby providing new insights for the therapeutic use of MSCs-derived products.