Miracle-dz/UniFAR-FTUs huggingface dataset:
L'etat viable non cultivable chez une bacterie responsable de toxi-infections alimentaires : campylobacter jejuni

L'etat viable non cultivable (vnc) d'une bacterie enteropathogene, campylobacter jejuni, a ete analyse sur trois souches presentant une perte des capacites a former des colonies, mais maintenant leur viabilite lorsqu'elles sont mises en suspension dans de l'eau filtree, sterilisee (microsom water). La viabilite des cellules viable non cultivable est etudie apres l'adaptation a c. Jejuni de deux techniques, la dvc et la double coloration ctc/dapi. Par sa rapidite et sa facilite d'interpretation, la coloration ctc/dapi apparait la plus interessante. La caracterisation de l'etat vnc aboutit aux resultats suivants : une reduction de la taille des cellules a l'etat vnc est observee par microscopie electronique a balayage, mais des cellules spiralees sont encore presentes apres trente jours d'incubation en microsom water. Les valeurs du ph interne, du potentiel de membrane et de la teneur en potassium diminuent chez les cellules vnc. De meme, les concentrations en atp et adp diminuent fortement, la charge energetique ultime des formes vnc n'etant pratiquement constituee que d'amp. Differentes experimentations permettent la mise en evidence d'une synthese de proteines dans les premieres heures d'incubation en microsom water, ces proteines interviennent dans la viabilite a long terme des cellules et dans l'acquisition d'une thermoresistance. Des inoculations de cellules vnc a des poussins, des souriceaux et des oeufs embryonnes, permettent un retour a l'etat cultivable de ces cellules. Les meilleurs resultats sont obtenus avec le modele oeuf embryonne. Des mesures d'indice d'association aux cellules hela, montrent une perte des capacites d'adhesion des cellules vnc. Toutefois, ces capacites sont restaurees apres passage sur les differents modeles animaux, indiquant le maintien de la pathogenicite de ces cellules. L'existence de cellules vnc de c. Jejuni capables de redevenir cultivable dans le tube digestif de differents animaux rend tout a fait probable l'intervention de ces cellules vnc dans les campylobacterioses digestives humaines.
The bamboos of the world: annotated nomenclature and literature of the species and the higher and lower taxa

Introduction. Subfamily Bambusoideae. Tribe Bambuseae. Subtribe Arundinariinae. Subtribe Thamnocalaminae. Subtribe Racemobambosinae. Subtribe Shibataeinae. Subtribe Bambusinae. Subtribe Melocanninae. Subtribe Hickeliinae. Subtribe Guaduinae. Subtribe Chusqueinae. Subtribe Arthrostylidiinae. Tribe Olyreae. Tribe Parianeae. Tribe Buergersiochloeae. Tribe Puelieae. Tribe Guaduelleae. Bibliography. Index.
Design of T cell epitope-driven IAV vaccines for swine using immunoinformatics tools (VAC2P.929)

Immunoinformatics tools have accelerated human vaccine design, but have not been applied extensively to animal vaccines. iVAX is a comprehensive toolkit to design genome-derived epitope-driven vaccines (GD-EDV) that uses EpiMatrix for epitope-mapping, among other algorithms. EpiMatrix was designed for use with human HLA. Using the pocket profile method, we created new T cell epitope-prediction matrices (PigMatrix) for porcine MHC (SLA). Integration of PigMatrix into iVAX now makes it possible to design GD-EDV for swine pathogens. We analyzed proteins from five influenza A viruses (IAV) representative of those prevalent in North American swine for highly conserved T cell epitopes. IFN-γ ELISpot responses to eleven 9mer epitopes, predicted to bind to class I SLA-1*0401, were measured using samples from pigs vaccinated with adjuvanted, whole-inactivated IAV. Together, these epitopes represented more than 50% of the response observed for whole virus re-stimulation. In further studies, we developed matrices for class I and II SLA alleles and predicted 28 conserved class I and 20 class II T cell epitopes from seven common IAV genomes. These epitopes were concatenated into two multiepitope IAV DNA vaccines for efficacy testing. These early results demonstrate the potential for immunoinformatics tools to develop rapid responses to infections affecting animals used for food. The new enhanced tools will facilitate the design of GD-EDV for swine diseases lacking effective vaccines.
MOLECULAR CHARACTERIZATION ON THE BASIS OF HA, NA AND M GENE REVEALED CHANGES IN CRITICAL AMINO ACID POSITIONS OF INFLUENZA A (H3N2) VIRUS CIRCULATING IN INDIA DURING 2011-2013

Influenza a (H1N1) virus is responsible for acute respiratory infection in human, which occasionally causes epidemic and rarely a pandemic. Full length DNA sequencing of HA, NA and M gene of H3N2 virus from year 2011-2013 was performed for determination of the circulating strains in India along with monitoring status of various mutations associated with drug resistance and virulence. Genetic analysis of HA gene of study samples in comparison to reference strain showed maximum amino acid changes in epitopes of high neutralization efficiencies (antigenic sites A, B and D) along with gain of glycosylation sites at position 45 (except single sample) and loss at position 126 (7 samples). NA gene has four amino acid changes in antibody binding region at position S367N, K369T, S332F and S334I along with gain and loss of glycosylation site at position 367 and 402 respectively whereas no change was observed in its active site. While comparison of deduced amino acid sequences of study samples with Indian strains from earlier years showed six amino acid changes in three antigenic sites on HA protein and three amino acid changes in NA protein epitope sequence. All these changes indicate that the H3N2 virus is undergoing antigenic drift and which may result in the emergence of new antigenic variants. We want to report that our study provided for the first time full length matrix gene sequence of influenza a (H3N2) virus from India.
The effects of Med12 variation upon cell cycle progression and differential gene expression

MED12 is a member of the Mediator complex that is a key regulator of tissue specific gene expression and moderates intracellular signaling via multiple developmental pathways. Sequence variation in the carboxy-terminus of MED12, which contains a PQL and Opa domain, is associated with X-linked mental retardation behavioral syndromes and schizophrenia. Unfortunately, the mechanism(s) through which sequence variation in the carboxy-terminus could alter vulnerability to neurodevelopmental and neuropsychiatric illnesses is yet unclear. In an effort to facilitate a better understanding of the role of the MED12 carboxyterminus in health and human behavior, a series of full-length and domain deleted overexpression constructs were developed. These overexpression constructs were used in tandem with a series of controls and RNA interference to examine the effects of the carboxy-terminus domains on cell cycle progression and gene expression using flow cytometry and genome-wide expression microarray analyses in a HEK293 cell model. Our results show that MED12 overexpression leads to G1 cell cycle exit, whereas deletion of the PQL domain and MED12 RNA interference has no effect upon cell cycle progression. Our data also show that MED12 expression level differentially affects early response antiviral gene expression and stress response mechanisms. These results are consistent with prior studies showing that MED12 has a key role in determining neuronal cell fate and with the theoretical understanding of the biological basis of psychosis. These results also lend further insight upon the pathways through which MED12 exerts its effects upon differentiation and disease pathogenesis, which may lead to new approaches to the treatment of MED12-related disorders.
Growth patterns, competition and coexistence in gap-phase regeneration under close-to-nature silviculture

The dominant European forest policy objective is to create multifunctional, mixed-species and uneven-aged forests. This objective includes the nature‐oriented conversion of monospecific plantations. To reach this objective, close-to-nature silviculture is applied. This generally entails natural regeneration in canopy gaps. In the Netherlands, experience with gap-phase regeneration is limited, making it difficult to asses the effect of close-to-nature management on forest development. In this thesis, this problem is addressed by investigating species coexistence between the four dominant species that occur in naturally regenerated canopy gaps in Douglas fir forest in the Netherlands. These species are Silver birch (Betula pendula Roth.), Japanese larch (Larix kaempferi Carr.), Scots pine (Pinus sylvestris L.) and Douglas fir (Pseudotsuga menziesii Mirb. Franco). During gap‐phase regeneration, saplings undergo self‐thinning. This affects species composition of the regeneration unit. Important factors in self‐thinning are species‐specific morphological growth patterns, emergent stand characteristics, the effect of stand characteristics on competitive relationships, and the effect of light availability on growth and mortality. Growth patterns differed between species and resulted in differences in the achieved height per unit biomass. Differences in mass‐based heights subsequently caused a vertical stratification in the regeneration unit which, in turn, affected the interspecific competition for light. However, results demonstrated a competitive response rather than a competitive effect. Surrounding saplings formed a functionally equivalent neighborhood, and target individuals responded mainly to their position in the canopy. Canopy position affected the radial growth of saplings. Mortality probabilities depended on radial growth, but did not differ between species despite a wide range in light demand. Scots pine did not show a relation between growth and mortality, even though it is highly responsive to light. Under continued autogenic development, a low-density top stratum of Silver birch and Japanese larch will develop, overtopping a declining number of Scots pine and a large number of increasingly dominant Douglas fir. This implies that Scots pine will be outcompeted by the other species, and Douglas fir will gain dominance in the future. Forest conversion by natural regeneration will therefore not lead to the desired mixed-species composition, but stands will eventually revert back to Douglas fir forest. Maintaining a diverse forest thus means the need for interventions in the early developmental stage.
Consumer-Centric Innovation for Mobile Apps Empowered by Social Media Analytics

Due to the rapid development of Internet communication technologies (ICTs), an increasing number of social media platforms exist where consumers can exchange comments online about products and services that businesses offer. The existing literature has demonstrated that online user-generated content can significantly influence consumer behavior and increase sales. However, its impact on organizational operations has been primarily focused on marketing, with other areas understudied. Hence, there is a pressing need to design a research framework that explores the impact of online user-generated content on important organizational operations such as product innovation, customer relationship management, and operations management. Research efforts in this dissertation center on exploring the co-creation value of online consumer reviews, where consumers’ demands influence firms’ decision-making. The dissertation is composed of three studies. The first study finds empirical evidence that quality signals in online product reviews are predictors of the timing of firms’ incremental innovation. Guided by the product differentiation theory, the second study examines how companies’ innovation and marketing differentiation strategies influence app performance. The last study proposes a novel text analytics framework to discover different information types from user reviews. The research contributes theoretical and practical insights to consumer-centric innovation and social media analytics literature. CONSUMER-CENTRIC INNOVATION FOR MOBILE APPS EMPOWERED BY SOCIAL MEDIA ANALYTICS
Mammalian Reproductive Biology

A unique interdisciplinary overview of the way mammals reproduce, this volume synthesizes research done by laboratory physiologists, behaviorists, population ecologists, and animal breeders. F. H. Bronson has drawn together the disparate literature in these areas to provide students and researchers with a comprehensive and biologically integrated approach to the study of mammalian reproduction. Each chapter presents a wealth of issues and questions, summarizing the current consensus on interpretations as well as viable alternatives under debate. The book is principally concerned with how environmental factors regulate reproduction. Bronson proposes that a mammal's reproductive performance routinely reflects simultaneous regulation by several environmental factors that interact in fascinatingly complex ways. Environment is defined broadly, and the chapters give equal weight to ecological and physiological factors when considering how variables such as food availability, ambient temperature, photoperiod, and social cues interact to regulate a mammal's reproduction. Particular attention is given to seasonal breeding, and a taxonomically arranged chapter underscores the importance of comparative and evolutionary biology to an understanding of mammalian reproduction. "Mammalian Reproductive Biology" is a powerful argument for the value and importance of interdisciplinary approaches to research. Its almost 1,500 references constitute the most comprehensive bibliography to date on this topic. Bronson also gives detailed consideration to promising areas for future research. Well organized, carefully planned, and clearly written, this book will become standard reading for scientists concerned with any aspect of mammalian biology.
Handbook of pests and diseases

Part 1 Damage to plants caused by pests and diseases: general considerations damage by insects, mites and nematodes how pests cause damage the seedlings damage to fruits and seeds, flowers and buds, leaves, roots and tubers damage by pathogens plant disease symptoms some common types of plant disease pest damage and disease assessment. Part 2 Choice of pesticides and dosage control of key pests and diseases - pest distribution: choice of pesticide control of key pests and diseases, control of leafhoppers and planthoppers, capsid bugs, lepidoptera, phytophagous mites, nematodes, viruses, bacteria, fungal pathogens on plant foliage, fungal pathogens on plant roots pest distribution. Part 3 Main crops: avocado banana brassicas capsicums cashew cassava citrus palm eggplant groundnut guava maize mango millet oil palm okra onions papaya passion fruits pineapple potato pulses rice rubber sesame sisal sorghum soybean sugar cane sweet potato teac tobacco tomato wheat yam cocoyam. Part 4 General and seedling pests: major insects and mite pests other pests of general crops. Part 5 Damage to harvested crops: damage by pests damage by disease stored produce infestation control. Appendices: preliminary examination and collection of diseased plants and insect pests notes on identification, preservation and shipment of insect specimens.
Physiological quality of algaroba seeds recovered from faeces of mules

A prevencao da expansao para areas ainda nao infestadas constitui uma medida prioritaria no controle da algaroba. A contaminacao de outras areas ocorre basicamente atraves dos rebanhos (endozoocoria), que ao consumirem seus frutos dispersam as sementes em areas distintas, facilitando o surgimento de novos povoamentos da planta invasora. Este trabalho foi realizado com o objetivo de determinar o tempo de passagem pelo trato gastrintestinal (TGI), a viabilidade e a qualidade fisiologica de sementes de algaroba ( Prosopis juliflora (Sw.) DC. ), recuperadas em fezes de muares. Tres muares adultos ficaram confinados em baias, sendo fornecido diariamente para cada um deles, como parte de sua dieta, um quilograma de frutos da referida especie invasora . Seus excrementos foram coletados, colocados sobre peneiras e lavados em agua corrente para retencao das sementes. As variaveis avaliadas foram: porcentagem de sementes excretadas, porcentagem de sementes com emissao da radicula e a qualidade fisiologica das sementes. Os muares, ao consumirem os frutos de P. juliflora , contribuem efetivamente para o avanco do processo de invasao biologica, sendo necessario receber um manejo adequado para que nao atuem como dispersores da referida invasora. A qualidade fisiologica das sementes de P. juliflora nao diminuiu com o tempo de permanencia no TGI. Para limpeza do trato digestivo de muares apos o consumo de frutos da referida invasora sao necessarios no minimo 10 dias. No campo, cerca de 20% das sementes excretadas podem ser preservadas viaveis nas fezes por mais de seis meses.
Altered PTEN expression as a diagnostic marker for the earliest endometrial precancerous changes

Introduction: Study was planned to investigate PTEN gene expression in endometrial hyperplasia and carcinoma as analyzed by immunohistochemistry. Material and Method: This study was conducted on 80 endometrial samples in the Department of Pathology, Gandhi Medical College, Bhopal (January 2012 to August 2016) Results: 5 out of 5 cases of proliferative endometrium, 3 out of 5 cases of secretory endometrium, 37 [84%] out of 44 cases of simple and complex endometrial hyperplasia without atypia, 8 out of 15 cases of simple and complex endometrial hyperplasia with atypia and 3 out of 11 cases of endometrial carcinoma showed positive PTEN expression. Conclusion: PTEN is a major gene involved in the pathogenesis of endometrial carcinoma. Our data suggest that decreased PTEN expression was a marker of earliest endometrial pre-cancer lesion and we propose that use of PTEN immunostaining in a clinical setting may be informative in identifying premalignant lesions that are likely to progress to carcinoma.
Pests of Fruit Crops: A Colour Handbook

Contents Introduction 1 Smaller insect orders 2 True bugs 3 Beetles and Weevils 4 True flies 5 Butterflies and Moths 6 Sawflies 7 Ants and wasps 8 Mites List of wild or ornamental host plants cited in the text Selected bibliography Glossary Host plant index Pest Index Main Plants include: apple, medlar, pear, quince, apricot, bullace, cherry, cherry-plum, damson, greengage, mirabelle, myrobalan, nectarine, peach, plum, blackberry, boysenberry, loganberry, raspberry, tayberry, blackcurrant, gooseberry, highbush blueberry, jostaberry, red currant and white currant, worcesterberry, grapevine, strawberry, almond, chestnut, cob and filbert nuts, hazelnut, pistachio, walnut, clementine, grapefruit, lemon, mandarin, orange, pomelo, tangerine, avocado, chinese persimmon, date plum, fig, hop, japanese persimmon, kaki, kiwifruit, mulberry, olive, oriental persimmon, pomegranate and prickly pear.
A new role of ATAC-HAT in dosage compensation.

In Drosophila similar to other eukaryotes, SAGA and ATAC HAT complexes exhibit different specificities in the targeted histone lysines and have essential but distinct functions as it is shown by the different phenotypes occurring in their absence. Besides Gnc5, Ada3 is also an important member of both types of HAT complexes. Loss of dADA3 function results in similar chromosome structural defects like the dGcn5 and dAda2a. The abnormal polytene chromosome structure of dAda3 mutants is visible most clearly on the X male chromosomes which appear bloated. Immunostaining indicates that the level of dADA3 protein is increased on the wild type male X. Nonetheless, the levels of K16 acetylated histone H4, a marker of dosage compensation is similar both in the mutant and wild type male X chromosomes. ATAC displays a preferential specificity towards acetylating histone H4. Besides already known acetylated lysine’s (H4K5 and K12) recent findings suggest a possible role of ATAC in H4K16 acetylation. This marker is well known as being specific for X male chromosome which is double transcribed in males compared to females. We have data that show a 2 fold increase in binding of dAda3 protein was observed on the male X that can be easily associated with increase transcriptional activity on the X. Also, the JIL-1 kinase that is not part of the MOF histone acetyltransferase has been shown to be implicated in dosage compensation. Phosphoryllation of H3 at Serine 10 has been shown to modulate chromosome condensation during mitosis. Reduced phosphorylation of H3S10 can also be observed in ATAC mutants.
Resource concentration by insects and implications for plant populations

1. The distribution of herbivores among plant patches may be an important factor determining plant population persistence. The resource concentration hypothesis proposes that herbivores are more abundant per unit plant at higher host plant densities and this has been found to occur in many systems. However, the opposite pattern, resource dilution, in which the herbivores are more abundant in low‐density patches and situations in which the number of insect herbivores per unit plant remains constant, also occurs.
New insights into the reproductive physiology and management of the female koala (Phascolarctos cinereus): factors affecting the control of the oestrous cycle

This study evaluated the practical application of faecal oestrogen analysis and the detection of oestrous behaviour to monitor ovarian cycles in future studies aimed at improving artificial breeding techniques in the koala. Faecal and plasma samples were collected from a total of 8 koalas (4 cycling and 4 non-cycling) every 2-4 days over a 6 week period or until females returned to oestrus. Total faecal oestrogens and plasma oestradiol-17β concentrations were evaluated to determine if faecal oestrogens gave an accurate index of plasma oestradiol-17β levels. Although spikes in total faecal oestrogens were detected in association with oestrous behaviour and elevated plasma oestradiol-17β, there were also elevated concentrations detected that did not correlate with plasma levels, despite allowing for a digestive tract processing lag affect. A possible explanation for this incongruence could be the combination of urine contamination and a long and differential digestion rate which ranges from 99 – 213 h depending on digesta particle size (99h 213h); this can lead to an inconsistent pooling of oestrogen levels in faecal samples. Whilst faecal oestrogens did not correlate with plasma oestradiol-17β to allow for an accurate estimate of cycle length or indicate the precise timing of oestrus, the individual mean faecal oestrogen concentrations did,