Miracle-dz/UniFAR-FTUs huggingface dataset:
FLAGELLAR ELONGATION AND SHORTENING IN CHLAMYDOMONAS

Flagella can be removed from the biflagellate Chlamydomonas and the cells begin to regenerate flagella almost immediately by deceleratory kinetics. Under usual conditions of deflagellation, more than 98% of all flagella are removed. Under less drastic conditions, cells can be selected in which one flagellum is removed and the other left intact. When only one of the two flagella is amputated, the intact flagellum shortens by linear kinetics while the amputated one regenerates. The two flagella attain an equal intermediate length and then approach their initial length at the same rate. A concentration of cycloheximide which inhibits protein synthesis permits less than one-third of each flagellum to form when both flagella are amputated. When only one is amputated in cycloheximide, shortening proceeds normally and the degree of elongation in the amputated flagellum is greater than if both were amputated in the presence of cycloheximide. The shortening process is therefore independent of protein synthesis, and the protein from the shortening flagellum probably enters the pool of precursors available for flagellar formation. Partial regeneration of flagella occurs in concentrations of cycloheximide inhibitory to protein synthesis suggesting that some flagellar precursors are present. Cycloheximide and flagellar pulse-labeling studies indicate that precursor is used during the first part of elongation, is resynthesized at mid-elongation, and approaches its original level as the flagella reach their initial length. Colchicine completely blocks regeneration without affecting protein synthesis, and extended exposure of deflagellated cells to colchicine increases the amount of flagellar growth upon transfer to cycloheximide. When colchicine is applied to cells with only one flagellum removed, shortening continues normally but regeneration is blocked. Therefore, colchicine can be used to separate the processes of shortening and elongation. Radioautographic studies of the growth zone of Chlamydomonas flagella corroborate previous findings that assembly is occurring at the distal end (tip growth) of the organelle.
Timing of geological events in the lunar highlands recorded in shocked zircon-bearing clasts from Apollo 16

Apollo 16 soil-like regolith breccia 65745,7 contains two zircon-bearing clasts. One of these clasts is a thermally annealed silica-rich rock, which mineralogically has affinities with the High Alkali Suite (Clast 1), and yields zircon dates ranging from 4.08 to 3.38 Ga. The other clast is a KREEP-rich impact melt breccia (Clast 2) and yields zircon dates ranging from 3.97 to 3.91 Ga. The crystalline cores of both grains, which yield dates of ca 3.9 Ga, have undergone shock pressure modification at less than 20 GPa. We interpret that the U-Pb chronometer in these zircon grains has been partially reset by the Imbrium basin-forming event when the clasts were incorporated into the Cayley Plains ejecta blanket deposit. The zircon grains in Clast 1 have been partially decomposed, resulting in a breakdown polymineralic texture, with elevated U, Pb and Th abundances compared with those in the crystalline zircon. These decomposed areas exhibit younger dates around 3.4 Ga, suggesting a secondary high-pressure, high-temperature event, probably caused by an impact in the local Apollo 16 highlands area.
Effect of fasting on resting metabolic rate and postprandial metabolic response in Silurus meridionalis

Resting metabolic rate in southern catfish of 2 and 5 day fasting groups were significantly higher than that of the 15 day fasting group (P < 0.05). After feeding, peak metabolic rate of specific dynamic action (SDA) of the 15 day fasting group was significantly lower than that of the 2 and 5 day fasting groups (P < 0.05). The duration of the SDA of the 15 day fasting group was significantly longer than that of the 2 day fasting group (P < 0.05) and the SDA coefficient of the 15 day fasting group was significantly lower than that of the 2 day fasting group (P < 0.05).
Gold, Silver, and Palladium Nanoparticles: A Chemical Tool for Biomedical Applications

Herein, the role of metal-based nanoparticles (NPs) in biomedical analysis and the treatment of critical deceases been highlighted. In the world of nanotechnology, noble elements such as the gold/silver/palladium (Au/Ag/Pd) NPs are the most promising emerging trend to design bioengineering materials that could to be employed as modern diagnostic tools and devices to combat serious diseases. NPs are considered a powerful and advanced chemical tool to diagnose and to cure critical ailments such as HIV, cancer, and other types of infectious illnesses. The treatment of cancer is the most significant application of nanotechnology which is based on premature tumor detection and analysis of cancer cells through Nano-devices. The fascinating characteristic properties of NPs—such as high surface area, high surface Plasmon resonance, multi-functionalization, highly stable nature, and easy processing—make them more prolific for nanotechnology. In this review article, the multifunctional roles of Au/Ag/Pd NPs in the field of medical science, the physicochemical toxicity dependent properties, and the interaction mechanism is highlighted. Due to the cytotoxicity of Ag/Au/Pd NPs, the conclusion and future remarks emphasize the need for further research to minimize the toxicity of NPs in the bio-medicinal field.
Investigating the Socially Shared Regulation of Learning in the Context of Programming Education

Students' ability to control and act upon their cognitive, behavioral, motivational, and metacognitive process is known as Self-regulation of learning (SRL). It has proved to be an effective strategy for academic programming success. However, the understanding of how programming students regulate their learning collaboratively deserves further investigation. The objective of this thesis is to study this process and use the findings to support the development and validation of a Computer-Supported Collaborative Learning (CSCL) environment grounded on the regulation of learning principles.
Creative Choice in Fifth Grade Computing Curriculum

We examine the effects of creative choice in fifth grade computer science curriculum on students' engagement/preferences, performance, and self-efficacy (n=107). This work is rooted in evaluating creativity as a tool to increase engagement in elementary school computer science education by studying a group of one hundred fifth grade students at a public charter school in California. The work occurred within a year long computing curriculum focused on introducing students to programming. To experimentally evaluate creative choice over a six week period, we created several instances of two variants of worksheets for the students - creative worksheets and more rigid worksheets, alternating control and experimental groupings. After the treatment, surveys and quizzes were used to evaluate students' experiences and learning. We found that students benefit from a blend of creative and structured instructions and can feel a sense of ownership with even limited versions of creative choice. In addition, student performance was not affected by our different treatments.
Use of Dual RNA-seq for Systems Biology Analysis of Zea mays and Aspergillus flavus Interaction

The interaction between Aspergillus flavus and Zea mays is complex, and the identification of plant genes and pathways conferring resistance to the fungus has been challenging. Therefore, the authors undertook a systems biology approach involving dual RNA-seq to determine the simultaneous response from the host and the pathogen. What was dramatically highlighted in the analysis is the uniformity in the development patterns of gene expression of the host and the pathogen during infection. This led to the development of a “stage of infection index” that was subsequently used to categorize the samples before down-stream system biology analysis. Additionally, we were able to ascertain that key maize genes in pathways such as the jasmonate, ethylene and ROS pathways, were up-regulated in the study. The stage of infection index used for the transcriptomic analysis revealed that A. flavus produces a relatively limited number of transcripts during the early stages (0 to 12 h) of infection. At later stages, in A. flavus, transcripts and pathways involved in endosomal transport, aflatoxin production, and carbohydrate metabolism were up-regulated. Multiple WRKY genes targeting the activation of the resistance pathways (i.e., jasmonate, phenylpropanoid, and ethylene) were detected using causal inference analysis. This analysis also revealed, for the first time, the activation of Z. mays resistance genes influencing the expression of specific A. flavus genes. Our results show that A. flavus seems to be reacting to a hostile environment resulting from the activation of resistance pathways in Z. mays. This study revealed the dynamic nature of the interaction between the two organisms.
An empirical examination of the determinants of honey consumption in Romania

Summary While not much attention has been paid in the international literature to the consumption of premium food products with enhanced quality properties in Central-Eastern European Countries (CEECs), the present survey aims to investigate consumer behaviour in Romania as regards ‘honey’ as an example of such a product. The research concludes that, despite the changes in eating habits induced by changes in the retail environment, honey remains a popular product, the purchase of which in bulk from producers and in open markets is still the dominant trend. The survey further identifies four main dimensions of honey-purchasing motivation: medical benefits of its consumption, dietary quality, ethical character of honey and suitability with food consumption lifestyle. Using these dimensions validated by factor analysis, three clusters of honey consumers in Romania emerged through cluster analysis: the common consumers, the younger consumers indifferent towards honey and the enthusiastic consumers, who are also more willing to pay premium prices for the organic type of honey. If the first segment may be the keenest on competitive prices, quality differentiation strategies can target the enthusiastic honey consumers. The distinctive features of Romanian consumers also include a low attention paid to labels, related in a low awareness of the energy content of honey. Quality cues are defined by search attributes of the bulk product (colour, taste, aroma, thickness), rather than credence attributes (warranties, brand name, country-of-origin). Marketers targeting this market should be aware of this scepticism towards label information, which necessitates more communication effort to built consumer trust.
Insights Into the Resistome of Bovine Clinical Mastitis Microbiome, a Key Factor in Disease Complication

Bovine clinical mastitis (CM) is one of the most prevalent diseases caused by a wide range of resident microbes. The emergence of antimicrobial resistance in CM bacteria is well-known, however, the genomic resistance composition (the resistome) at the microbiome-level is not well characterized. In this study, we applied whole metagenome sequencing (WMS) to characterize the resistome of the CM microbiome, focusing on antibiotics and metals resistance, biofilm formation (BF), and quorum sensing (QS) along with in vitro resistance assays of six selected pathogens isolated from the same CM samples. The WMS generated an average of 21.13 million reads (post-processing) from 25 CM samples that mapped to 519 bacterial strains, of which 30.06% were previously unreported. We found a significant (P = 0.001) association between the resistomes and microbiome composition with no association with cattle breed, despite significant differences in microbiome diversity among breeds. The in vitro investigation determined that 76.2% of six selected pathogens considered “biofilm formers” actually formed biofilms and were also highly resistant to tetracycline, doxycycline, nalidixic acid, ampicillin, and chloramphenicol and remained sensitive to metals (Cr, Co, Ni, Cu, Zn) at varying concentrations. We also found bacterial flagellar movement and chemotaxis, regulation and cell signaling, and oxidative stress to be significantly associated with the pathophysiology of CM. Thus, identifying CM microbiomes, and analyzing their resistomes and genomic potentials will help improve the optimization of therapeutic schemes involving antibiotics and/or metals usage in the prevention and control of bovine CM.
Turing and Benjamin–Feir instability mechanisms in non-autonomous systems

The Turing and Benjamin–Feir instabilities are two of the primary instability mechanisms useful for studying the transition from homogeneous states to heterogeneous spatial or spatio-temporal states in reaction–diffusion systems. We consider the case when the underlying reaction–diffusion system is non-autonomous or has a base state which varies in time, as in this case standard approaches, which rely on temporal eigenvalues, break down. We are able to establish respective criteria for the onset of each instability using comparison principles, obtaining inequalities which involve the in general time-dependent model parameters and their time derivatives. In the autonomous limit where the base state is constant in time, our results exactly recover the respective Turing and Benjamin–Feir conditions known in the literature. Our results make the Turing and Benjamin–Feir analysis amenable for a wide collection of applications, and allow one to better understand instabilities emergent due to a variety of non-autonomous mechanisms, including time-varying diffusion coefficients, time-varying reaction rates, time-dependent transitions between reaction kinetics and base states which change in time (such as heteroclinic connections between unique steady states, or limit cycles), to name a few examples.
Vaccines Against Antimicrobial Resistance

In the last century, life expectancy has increased considerably, thanks to the introduction of antibiotics, hygiene and vaccines that have contributed to the cure and prevention of many infectious diseases. The era of antimicrobial therapy started in the nineteenth century with the identification of chemical compounds with antimicrobial properties. However, immediately after the introduction of these novel drugs, microorganisms started to become resistant through different strategies. Although resistance mechanisms were already present before antibiotic introduction, their large-scale use and mis-use have increased the number of resistant microorganisms. Rapid spreading of mobile elements by horizontal gene transfer such as plasmids and integrative conjugative elements (ICE) carrying multiple resistance genes has dramatically increased the worldwide prevalence of relevant multi drug-resistant human pathogens such as Staphylococcus aureus, Neisseria gonorrhoeae, and Enterobacteriaceae. Today, antimicrobial resistance (AMR) remains one of the major global concerns to be addressed and only global efforts could help in finding a solution. In terms of magnitude the economic impact of AMR is estimated to be comparable to that of climate global change in 2030. Although antibiotics continue to be essential to treat such infections, non-antibiotic therapies will play an important role in limiting the increase of antibiotic resistant microorganisms. Among non-antibiotic strategies, vaccines and therapeutic monoclonal antibodies (mAbs) play a strategic role. In this review, we will summarize the evolution and the mechanisms of antibiotic resistance, and the impact of AMR on life expectancy and economics.
Bacterial pathogens in rainbow trout, Oncorhynchus mykiss (Walbaum), reared at Danish freshwater farms.

During a 2-year period, bacterial fish pathogens were monitored on five rainbow trout, Oncorhynchus mykiss (Walbaum), freshwater farms in Denmark. A total of 1206 fish were examined and 361 bacterial isolates were identified phenotypically. Enteric redmouth disease, furunculosis and rainbow trout fry syndrome/coldwater disease were recorded. Infections caused by Flavobacterium psychrophilum occurred most frequently, but only one outbreak of enteric redmouth disease caused by Yersinia ruckeri serotype O1 and one of furunculosis caused by Aeromonas salmonicida were recorded during the monitoring period. Flavobacterium psychrophilum was isolated on all farms, both during disease outbreaks and from fish without any signs of disease. Serological investigations of F. psychrophilum showed that serotype Th was the dominant serotype found. The serotypes Th and Fd were involved in disease outbreaks of fry and larger fish. All isolates of F. psychrophilum showed proteolytic activities; however, a few isolates, belonging to serotype FpT did not degrade elastin and were not associated with mortality. Increasing resistance problems to oxytetracycline were demonstrated. More than half of the F. psychrophilum isolates showed resistance to oxolinic acid and oxytetracycline. No antibiotic resistant isolates were found among Y. ruckeri and A. salmonicida.
Applications of Resting-State fNIRS in the Developing Brain: A Review From the Connectome Perspective

Early brain development from infancy through childhood is closely related to the development of cognition and behavior in later life. Human brain connectome is a novel framework for describing topological organization of the developing brain. Resting-state functional near-infrared spectroscopy (fNIRS), with a natural scanning environment, low cost, and high portability, is considered as an emerging imaging technique and has shown valuable potential in exploring brain network architecture and its changes during the development. Here, we review the recent advances involving typical and atypical development of the brain connectome from neonates to children using resting-state fNIRS imaging. This review highlights that the combination of brain connectome and resting-state fNIRS imaging offers a promising framework for understanding human brain development.
Diabetic Nephropathy: Perspective on Extracellular Vesicles

Diabetic nephropathy (DN) is a major microvascular complication of diabetes mellitus. It is the most frequent cause of end-stage renal disease with no definitive therapy available so far. Extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, are nano- and micron-sized heterogeneous vesicles that can be secreted by almost all cell types. Importantly, EVs contain many biologically active materials, such as RNAs, DNAs, proteins, and lipids, from their parental cells, which can be transported to their recipient cells to mediate intercellular communication and signaling. Accumulating studies demonstrated that EVs, mainly exosomes and microvesicles, participated in the pathophysiological process of DN. Recently emerging studies also found that the contents of EVs in the urine (miRNAs, mRNAs, and proteins) could be used as potential biomarkers for DN. Therefore, in this mini-review, the generation, isolation methods, and biological function of EVs were introduced, and then the current information about the mechanism and the diagnostic value in the development of DN was summarized. Moreover, the review also discussed the future challenges of exploring the role of EVs in kidney disease.
Hands-on Security Testing in a University Lab Environment

In this paper, we present a novel approach to providing students with hands-on learning experiences for security testing. Specifically, we give details of a Raspberry Pi-based lab architecture which we developed as part of a security course, and provide example lab tasks that such an architecture affords. We provide evidence that introducing such hands-on labs within the security course directly improved student performance. Finally, we compare our approach to other existing approaches of which we are aware that are based on virtual machines and networks.
Impacts of Block-based Programming on Young Learners' Programming Skills and Attitudes in the Context of Smart Environments

In computer programming education, showing the application of programming in reality has become a common way to introduce it to young learners. However, we have limited knowledge of how best to utilize smart objects and environments to foster the learners' programming skills and develop a positive attitude towards programming. My research focuses on filling this gap by presenting an educational block-based programming tool that brings together the hot topic of smart environments and the visual programming paradigm. The end goal is empirically investigating the impacts of state-of-the-art smart technologies together with block-based programming on the learners' programming skills and attitudes towards programming in non-formal learning environments.
CONTINUOUS CHAOS—FOUR PROTOTYPE EQUATIONS

If oscillation is the typical behavior of 2-dimensional dynamical systems (Euclidean and on manifolds), then chaos, in the same way, characterizes 3-dimensional continuous systems. First a method t o obtain chaos in degenerate (relaxation type) dynamical systems in two variables is outlined whereby five basic flow patterns emerge. Second, following a piecewise linear degenerate equation, four prototypically simple quadratic differential equations in three variables that realize nondegenerate analogs of those five flows are presented. Finally a possible equation for an even higher type of qualitative behavior beyond chaos is proposed.
Cortical resin monoterpenes in Sitka spruce and resistance to the white pine weevil, Pissodesstrobi (Coleoptera: Curculionidae)

To determine whether monoterpene content of Sitka spruce, Piceasitchensis (Bong.) Carr. , could be correlated with resistance to the white pine weevil, Pissodesstrobi Peck, the relative amounts of cortical monoterpenes of five 50-year-old resistant and five susceptible trees of the same age were analyzed. The resistant Sitka spruce had significantly lower β-phellandrene and higher β-pinene and 3-carene content than the susceptible trees. This monoterpene profile could be used as an indicator of resistant trees.
Expansão e mortalidade de ramos em espécies arbóreas do cerrado sensu stricto

The study of shoot growth and mortality provide essential information on tree growth dynamics. This study shows the patterns of growth and mortality in shoots of ten common tree species in a "cerrado" sensu stricto. Mean annual branch growth was variable among the species and ranged from 1 to 17 cm per year. Miconia pohliana Cogn. and Vochysia thyrsoidea Pohl were the ones with the highest rates of branch growth. Most shoot mortality took place during the dry season. In conclusion, shoot loss is a very common phenomenon in "cerrado" tree species and their impacts on productivity studies and growth analysis should be considered.
Environmental controls on the distribution of wildfire at multiple spatial scales

Despite its widespread occurrence globally, wildfire preferentially occupies an environmental middle ground and is significantly less prevalent in biomes characterized by environmental extremes (e.g., tundra, rain forests, and deserts). We evaluated the biophysical “environmental space” of wildfire from regional to subcontinental extents, with methods widely used for modeling habitat distributions. This approach is particularly suitable for the biogeographic study of wildfire, because it simultaneously considers patterns in multiple factors controlling wildfire suitability over large areas. We used the Maxent and boosted regression tree algorithms to assess wildfire–environment relationships for three levels of complexity (in terms of inclusion of variables) at three spatial scales: the conterminous United States, the state of California, and five wildfire-prone ecoregions of California. The resulting models were projected geographically to obtain spatial predictions of wildfire suitability and were also .