Miracle-dz/UniFAR-FTUs huggingface dataset:
Calcium pyrophosphate dihydrate gout and other crystal deposition diseases

The number of crystal or birefringent particles associated with arthritis is increasing, and a uniform taxonomy is needed. The term gout has been proposed as a generic term for these diseases based on historical, clinical, and crystallographic reasons. Calcium pyrophosphate dihydrate gout follows monosodium urate gout in frequency, and its spectrum of clinical manifestations continues to grow. Familial calcium pyrophosphate dihydrate gout was described for the first time in kindreds studied in England and Tunisia; new Jewish and Spanish kindreds were also reported. Type I collagen was shown to nucleate nativelike calcium pyrophosphate dihydrate crystals, and pyrophosphate elaboration was explored in cartilage explants in an attempt to reproduce the in vivo metabolic or endocrine disorders associated with calcium pyrophosphate dihydrate gout. The effect of pyrophosphatase and different cofactors such as magnesium in dissolving calcium pyrophosphate dihydrate crystals was investigated. High-resolution electron microscopy was used to study the interrelation between apatite and other basic calcium phosphate crystals in apatite gout. Raman microscopy was applied for the first time to identify crystals in biologic specimens. A simple and specific technique for basic calcium phosphate crystal identification is necessary to understand the relationship between different calcium phosphate crystals and osteoarthritis. Several reports about children and young patients with primary oxalate gout described the effect of oxalate on eyes, periodontal tissues, and bone. Multicenter studies showed poor results of renal transplantation, but favored combined liver and renal transplantation. Reports of patients with vascular cryoglobulin crystalline deposits as well as patients with synovitis due to different foreign bodies were published. Foreign bodies have been identified with both simple and sophisticated crystallographic techniques.
Isolation and genetic characterization of hantaviruses carried by Microtus voles in China

To gain more insights into hantavirus distribution in China, Microtus fortis were caught in Jilin province and M. maximowiczii in the Inner Mongolia Autonomous Region. Hantavirus specific RNA was detected by RT‐PCR in 3 out of 26 M. fortis and 5 out of 64 M. maximowiczii. Two hantaviruses (Fusong‐Mf‐682 and Yakeshi‐Mm‐59) were isolated successfully in cell culture and their S and M segment nucleotide sequences were determined. Phylogenetic analysis of the S and M segment sequences revealed that the Mf‐originated strains from Fusong were closely related to Vladivostok hantavirus (VLAV) with 99% nucleotide identity, but differed from the Yakeshi‐Mm strains, with an amino acid divergence of more than 8.8% for the N protein and 11.8% for the GnGc proteins. Yakeshi‐Mm strains were closely related to the Khabarovsk hantavirus (KHAV) isolated earlier from M. fortis in Khabarovsk, with an amino acid sequence identity of more than 98.4% for the S segment and 95.6% for the M segment. On phylogenetic trees, Yakeshi‐Mm strains clustered together with KHAV and Topografov virus (TOPV) carried by Lemmus sibiricus. The results suggest that the hantavirus carried by M. fortis in China belongs to VLAV type and should be considered as a distinct hantavirus species. They also suggest that M. fortis is the natural host of VLAV (including Fusong‐Mf strains), whereas M. maximowiczii is the natural host of KHAV including Yakeshi‐Mm strains. Thus, in addition to Hantaan, Seoul, Dabieshan and Puumala‐like Hokkaido viruses, at least two other hantaviruses, namely KHAV and VLAV, are circulating in China. J. Med. Virol. 80:680–688, 2008. © 2008 Wiley‐Liss, Inc.
Effects of Immunomodulators on Ecto‐5′‐Nucleotidase Activity on Blood Mononuclear Cells In Vitro

In this study the effects of immunomodulators on the ecto‐5′‐nucleotidase (ecto‐5′‐NT) activity on blood mononuclear cells (BMC) were examined in vitro. Interleukin‐4 (IL‐4) and interferon‐γ (IFN‐γ) decreased the level of ecto‐5′‐NT activity on BMC whereas prostaglandin E2 (PGE2) increased the ecto‐5′‐NT level. All three immunomodulators influenced the ecto‐5′‐NT activity of isolated monocytes whereas only IL‐4 and PGE2 had an effect on the enzyme level on isolated lymphocytes. The effect was dependent upon protein synthesis. The effect was dose dependent: IL‐4 was effective at concentrations down to 0.5 U/ml, IFN‐γ down to 40 U/ml and PGE2 at nanomolar concentrations. These data indicate that immunomodulators may also take part in the regulation of ecto‐5′‐NT activity on BMC in vivo.
Hematopoietic Commitment during Embryogenesis

Abstract: Hematopoiesis develops initially as discrete blood islands in the extraembryonic yolk sac of the embryo. These blood islands consist of clusters of primitive erythrocytes surrounded by developing angioblasts that ultimately form the yolk sac vasculature. The close developmental association of these early hematopoietic and endothelial cells has led to the hypothesis that they develop from a common precursor, a cell known as the hemangioblast. Using a developmental model system based on the in vitro differentiation capacity of embyronic stem (ES) cells, we have identified a precursor with the capacity to generate endothelial as well as primitive and definitive hematopoietic progeny. The developmental potential of this precursor population suggests that it represents the in vitro equivalent of the hemangioblast.
Ethnic Hierarchy and Public Attitudes towards Immigrants in Russia

Explanations of anti-immigrant attitudes in Europe have been centred around the labour market competition and group threat theories. The paper tests these theories with the data from Russia and finds some support for the group threat theory. Attitudes towards several immigrant ethnic groups are analysed separately. While Russians generally accept Ukrainians and Moldovans as their potential neighbours, they are more hostile to immigrants from the Caucasus and Central Asia. This ethnic hierarchy is shared by all large ethnic groups populating Russia. The analysis of regional level covariates of anti-immigrant sentiment shows that higher concentration of immigrants is associated with more negative attitudes towards most immigrant groups, except Ukrainians. Poorer regions are more xenophobic. The predictive power of statistical models explaining anti-immigrant prejudice is considerably lower in Russia compared to Western European countries. The paper discusses to what extent standard explanations of anti-immigrant attitudes in Europe can be applied in Russia.
Exploring the crowded central region of ten Galactic globular clusters using EMCCDs - Variable star searches and new discoveries

Aims. We aim to obtain time-series photometry of the very crowded central regions of Galactic globular clusters; to obtain better angular resolution thanhas been previously achieved with conventional CCDs on ground-based telescopes; and to complete, or improve, the census of the variable star population in those stellar systems. Methods. Images were taken using the Danish 1.54-m Telescope at the ESO observatory at La Silla in Chile. The telescope was equipped with an electron-multiplying CCD, and the short-exposure-time images obtained (ten images per second) were stacked using the shift-and-add technique to produce the normal-exposure-time images (minutes). Photometry was performed via difference image analysis. Automatic detection of variable stars in the field was attempted. Results. The light curves of 12 541 stars in the cores of ten globular clusters were statistically analysed to automatically extract the variable stars. We obtained light curves for 31 previously known variable stars (3 long-period irregular, 2 semi-regular, 20 RR Lyrae, 1 SX Phoenicis, 3 cataclysmic variables, 1 W Ursae Majoris-type and 1 unclassified) and we discovered 30 new variables (16 long-period irregular, 7 semi-regular, 4 RR Lyrae, 1 SX Phoenicis and 2 unclassified). Fluxes and photometric measurements for these stars are available in electronic form through the Strasbourg astronomical Data Center.
Incorporating geodiversity into conservation decisions

In a rapidly changing climate, conservation practitioners could better use geodiversity in a broad range of conservation decisions. We explored selected avenues through which this integration might improve decision making and organized them within the adaptive management cycle of assessment, planning, implementation, and monitoring. Geodiversity is seldom referenced in predominant environmental law and policy. With most natural resource agencies mandated to conserve certain categories of species, agency personnel are challenged to find ways to practically implement new directives aimed at coping with climate change while retaining their species‐centered mandate. Ecoregions and ecological classifications provide clear mechanisms to consider geodiversity in plans or decisions, the inclusion of which will help foster the resilience of conservation to climate change. Methods for biodiversity assessment, such as gap analysis, climate change vulnerability analysis, and ecological process modeling, can readily accommodate inclusion of a geophysical component. We adapted others’ approaches for characterizing landscapes along a continuum of climate change vulnerability for the biota they support from resistant, to resilient, to susceptible, and to sensitive and then summarized options for integrating geodiversity into planning in each landscape type. In landscapes that are relatively resistant to climate change, options exist to fully represent geodiversity while ensuring that dynamic ecological processes can change over time. In more susceptible landscapes, strategies aiming to maintain or restore ecosystem resilience and connectivity are paramount. Implementing actions on the ground requires understanding of geophysical constraints on species and an increasingly nimble approach to establishing management and restoration goals. Because decisions that are implemented today will be revisited and amended into the future, increasingly sophisticated forms of monitoring and adaptation will be required to ensure that conservation efforts fully consider the value of geodiversity for supporting biodiversity in the face of a changing climate.
Droperidol exerts dual effects on repolarization and induces early afterdepolarizations and triggered activity in rabbit Purkinje fibers.

This study was designed to clarify discrepancies concerning the effects of droperidol on cardiac repolarization. Myocardial electrical activity was recorded by using microelectrode technique in rabbit Purkinje fibers and guinea pig ventricular muscle. In Purkinje fibers stimulated at 60 pulses/min, low concentrations (0.01-0.3 microM) of droperidol increased in a dose-dependent fashion action potential duration (APD) without altering the other parameters. At 1 and 3 microM, droperidol led to the reversal of the prolonging effect. The highest concentrations used (10 and 30 microM), produced shortening in APD at 50% repolarization concomitantly with a significant decrease in Vmax, action potential amplitude and resting membrane potential. Inexcitability occurred in 4 of 15 preparations exposed to 30 microM. In 8 of 15 Purkinje fibers, the prolonging effect induced by low concentrations was so important that APD exceeded the 1000-msec period of basal stimulation and early afterdepolarizations (EADs) and triggered activity developed. In guinea pig ventricular muscle, these effects were notably less pronounced. Prolongation of action potential showed a reverse use-dependence (i.e., much greater at the lowest stimulation frequencies), whereas Vmax depression was use-dependent. Decreasing extracellular K concentration from 4.0 to 2.7 mM enhanced the incidence of EADs in Purkinje fibers, whereas elevating the K concentration from 2.7 to 5.4 mM abolished them completely and shortened drastically APD. EADs were also eliminated by increasing magnesium concentration from 1 to 5 mM. Addition of isoproterenol favored EADs, whereas these were suppressed at plateau level by exposure to 0.3 microM nifedipine. The results indicate that in rabbit Purkinje fibers, droperidol exerts a dual effect on repolarization, prolongation with low concentrations with development of EADs and subsequent triggered activity. These abnormalities were suppressed at high concentrations concomitantly with a marked depression of other characteristics. These observations suggest multiple ionic channel activities and further studies are required to precise the underlying mechanisms at channel level.
The validity of self-reports of alcohol consumption: state of the science and challenges for research.

AIMS To review three topics pertaining to the validity of alcohol self-reports: factors that influence response accuracy; the relative merits of different self-report approaches; and the utility of using alternative measures to confirm verbal reports. FINDINGS Response behavior is influenced by the interaction of social context factors, respondent characteristics, and task attributes. Although research has advanced our knowledge about self-report methods, many questions remain unanswered. In particular, there is a need to investigate how task demands interact with different patterns of drinking behavior to affect response accuracy. There is also a continuing need to use multiple data sources to examine the extent of self-report response bias, and to determine whether it varies as a function of respondent characteristics or assessment timing. CONCLUSION Self-report methods offer a reliable and valid approach to measuring alcohol consumption. The accuracy of such methods, however, can be improved by research directed at understanding the processes involved in response behavior.
An Activity-Embedding Approach for Next-Activity Prediction in a Multi-User Smart Space

Since the advent of the IoT era, various IoT devices have proliferated, transforming ordinary spaces into smart spaces such as smart home, smart office, and smart building. To provide user-friendly service to people, the majority of previous studies have focused on activity recognition and prediction in singleuser environments such as ambient assisted living (AAL) and activities of daily living (ADL). However, unlike single-user environments, many real world environments are comprised of multiple activities occurring concurrently in a multi-user smart space. In the presence of multiple activities and multiple users, the process of next-activity prediction rarely produces just a single candidate for the next activity. Thus, we present in this paper an approach that generates multiple next- activity candidates. Our approach is motivated by a specific word-embedding algorithm that is typically used for natural language processing (NLP) to map words into a vector space. By using a similar embedding approach in a multi-user smart space, we map activities to vector coordinates in a vector space. After the vectorization, a long short-term memory (LSTM) network can be trained to predict a single vector coordinate for next activity from a group of previously occurring activities; then from this single prediction, multiple next-activity candidates are selected by choosing several vectors near the LSTM's single output. We tested our approach using real data generated from the multi- user smart space testbed on our campus. After the embedding of activities into a vector space, we were able to find semantically meaningful relations between the resultant vectors. In addition, next- activity prediction had a success rate of approximately 82%. Our activity embedding and next- activity prediction method can be utilized together in multi-user smart spaces to develop smart service systems such as a service recommendation system.
Metabolism of megestrol acetate in vitro and the role of oxidative metabolites

Abstract 1. There is limited knowledge regarding the metabolism of megestrol acetate (MA), as it was approved by FDA in 1971, prior to the availability of modern tools for identifying specific drug-metabolizing enzymes. We determined the cytochrome P450s (P450s) and UDP-glucuronosyltransferases (UGTs) that metabolize MA, identified oxidative metabolites and determined pharmacologic activity at the progesterone, androgen and glucocorticoid receptors (PR, AR and GR, respectively). 2. Oxidative metabolites were produced using human liver microsomes (HLMs), and isolated for mass spectral (MS) and nuclear magnetic resonance (NMR) analyses. We screened recombinant P450s using MA at 62 μM (HLM Km for metabolite 1; M1) and 28 μM (HLM Km for metabolite 2; M2). UGT isoforms were simultaneously incubated with UDPGA, nicotinamide adenine dinucleotide phosphate (NADPH), CYP3A4 and MA. Metabolites were evaluated for pharmacologic activity on the PR, AR and GR. CYP3A4 and CYP3A5 are responsible for oxidative metabolism of 62 μM MA. 3. At 28 μM substrate concentration, CYP3A4 was the only contributing enzyme. Mass spectral and NMR data suggest metabolism of MA to two alcohols. After oxidation, MA is converted into two secondary glucuronides by UGT2B17 among other UGTs. MA, M1 and M2 had significant pharmacologic activity on the PR while only MA showed activity on the AR and GR.
Harvey Cushing and the regulation of blood pressure in giraffe, rat and man: introducing ‘Cushing's mechanism’

The fundamental mechanism that underlies essential hypertension is a high total peripheral resistance. We review here possible origins of high total peripheral resistance in physiologically hypertensive giraffes, spontaneously hypertensive rats and humans with essential hypertension. We propose that a common link could be reduced brainstem perfusion, as first suggested by Cushing in 1901. Any tendency towards reduction of cerebral blood flow to the cardiovascular control centres in rest and sleep will be prevented by activation of a response arising in the brainstem. The response will proportionately increase systemic blood pressure and return cerebral blood flow to a new homeostatic level. New evidence we review here supports this idea and leads us to suggest that central regulation of blood pressure has two components: the classic Cushing's response, which is a terminal event, and a Cushing's mechanism, which is a physiological mechanism for long‐term control of mean arterial pressure. In giraffes, Cushing's mechanism is activated by increasing neck length during growth and subsequent gravitational hypotension that stimulates a rise in basal arterial blood pressure. In man and rats, the mechanism is activated by narrowing of the arteries supplying the brainstem. If we are correct, future successful treatment of essential hypertension in man will include methods of reducing cerebral arterial resistance.
Telephone interviewing: is it compatible with interpretive phenomenological research?

Abstract The telephone has long been used as a medium of communication. In more recent years the telephone has become a legitimate tool in marketing and survey research (Barriball et al. 1996). Telephone interviewing is becoming an increasingly popular form of interview for qualitative research (Carr & Worth 2001). Whilst there have been discussions in the literature on logistical advantages and disadvantages of telephone interviewing, there has been little debate as to whether this form of interview is compatible with qualitative health research. Much of the literature reporting this interview method is based on quantitative or structured questionnaire style research under the guise of ‘qualitative’ research. So the question remains: Is the telephone interview compatible with interpretive phenomenological research? This paper describes how telephone interviewing was used in a recently conducted interpretive phenomenological study, and argues that this is a methodologically and economically valuable data collection technique in qualitative research. Qualitative researchers should not rely exclusively on the face-to-face interview, as the telephone interview can be an equally valuable data collection approach.
Acute effects of two different initial heart rates on testing the Repeated Sprint Ability in young soccer players.

AIM The aim of this paper was to investigate the acute effects of two different initial heart rates intensities when testing the repeated sprint ability (RSA) performances in young soccer players. METHODS Since there are many kinds of pre-match warm-ups, we chose to take as an absolute indicator of internal load the heart rate reached at the end of two different warm-up protocols (60 vs. 90% HRmax) and to compare the respective RSA performances. The RSA tests were performed on fifteen male soccer players (age: 17.9±1.5 years) with two sets of ten shuttle-sprints (15+15 m) with a 1:3 exercise to rest ratio, in different days (randomized order) with different HR% (60 & 90% HRmax). In order to compare the different sprint performances a Fatigue Index (FI%) was computed, while the blood lactate concentrations (BLa-) were measured before and after testing, to compare metabolic demand. RESULTS Significant differences among trials within each sets (P<0.01) were found. Differences between sets were also found, especially comparing the last five trials for each set (Factorial ANOVA; P<0.01), effect size values confirming the relevance of these differences. Although the BLa- after warm-up was higher (36%) between 90% vs. 60% HRmax, after the RSA test the differences were considerably low (7%). CONCLUSION Based on physiological information's this methodological approach (testing with initial 90%HRmax) reflects more realistically the metabolic background in which a soccer player operates during a real match. This background may be partially reproduced by warming up protocols that, by duration and metabolic commitment, can reproduce conveniently the physiological conditions encountered in a real game (e.g. HRmax≈85-95%; BLa->4 mmol/L(-1)).
Microendoscopic posterior cervical laminoforaminotomy for unilateral radiculopathy: results of a new technique in 100 cases.

OBJECT In this report the author presents surgery-related outcomes after application of a new technique. A posterior microendoscopic laminoforaminotomy was used for the surgical treatment of unilateral cervical radiculopathy secondary to intervertebral disc herniations and/or spondylotic foraminal stenosis. The results of this procedure are compared with those achieved using traditional laminoforaminotomy and anterior cervical discectomy with or without fusion. METHODS One hundred consecutive patients who experienced unilateral cervical radicular syndromes, which were refractory to conservative therapy, and in whom imaging studies had confirmed lateral canal or foraminal compression, underwent surgical treatment. An endoscopy-assisted posterior laminoforaminotomy was performed using a microendoscopic visualization system for removal of herniated disc and foraminal decompression while the patient was in the sitting position. Excellent or good results were obtained in 97 patients. who returned to their preoperative employment and baseline level of physical activity. One patient returned to work but was unable to perform at baseline level; two patients returned to prior sedentary work but continued to have some activity-related pain and paresthesias. Two patients reported experiencing intermittent paresthesias or numbness, but this did not limit their activities. There were two cases of dural punctures, one case of superficial wound infection, and no deaths. CONCLUSIONS The microendoscopic posterior laminoforaminotomy is an effective alternative for the treatment of unilateral cervical radiculopathy secondary to lateral or foraminal disc herniations or spondylosis. In this group of patients, it is preferable because it does not require the sacrifice of a cervical motion segment, has a low incidence of complications, and is associated with a much quicker return to unrestricted full activity than that obtained with other techniques.