Miracle-dz/UniFAR-FTUs huggingface dataset:
The NCI Biospecimen Research Network

Since access to high‐quality human biospecimens is a critical resource to support genomic‐ and proteomic‐base studies, the NCI established the Office of Biorepositories and Biospecimen Research (OBBR) to coordinate biospecimen research‐related policies and practices. The NCI Biospecimen Research Network (BRN) of the OBBR is conducting bimolecular studies for the development of data‐driven, evidence‐based protocols for various specimen types and molecular analysis platforms. Two current projects examine the effects of pre‐analytical variables on downstream gene expression. The first project examines the effect of intra‐operative ischemic times from 20 to 50 minutes on gene expression profiles in colon cancer tissues. Tissue samples were microdissected, RNA purified, and quantity/quality determined. After RNA amplification and labeling, differential gene expression for ischemia were determined using Affymetrix Human Genome U133 GeneChips. Genes differentially expressed will be validated using quantitative RT‐PCR. The second project compares histology and recovery of RNA for frozen, FineFix‐fixed, paraffin‐embedded (FiFPE), ethanol‐fixed, paraffin‐embedded (EFPE), and formalin‐fixed, paraffin‐embedded (FFPE) kidney and prostate tissue. Histological quality is comparable for FiFPE, EFPE, and FFPE tissue and is superior to frozen tissue. By RT‐PCR for beta actin, RNA recovery is comparable for frozen and FiPE and are both superior to EFPE and FFPE. RNA recovery will be further assessed by qRT‐PCR for cyclophilin A, beta actin, HPRT, and GAPDH.
Ankle Fusion in a High Risk Population: An Assessment of Nonunion Risk Factors

Between July 1992 and April 1996, 88 ankle fusions were performed at our institution. Sixty-seven of these had adequate follow-up for evaluation for union of the fusion, including adequate records and/or radiographs. The average age of patients was 43 years. There were 37 men and 24 women. The charts were reviewed to determine what level of trauma had resulted in posttraumatic arthritis (low energy, high energy, or open fracture). Alcohol use, drug abuse, diabetes, peripheral vascular disease, psychiatric history, smoking, or technical problems were also assessed. A chi-square analysis was used to evaluate the statistical significance. Nineteen of sixty-seven ankle fusions progressed to nonunion (28%). Eighty-five percent of the patients had posttraumatic arthritis. Among 17 patients with a history of open trauma, nine patients developed a nonunion (P < 0.03). A trend toward significance was noted for patients who were smokers, drank alcohol, had diabetes, had a psychiatric disorder, or used illegal drugs. Even with current techniques, this study demonstrates that a high risk population in a trauma center is at risk for nonunion after an ankle fusion caused by multiple risk factors, including a history of open trauma, tobacco use, alcohol use, illegal drug use, a history of psychiatric disorders, or diabetes.
Bileclar – Medical Information

Medicinal plants with the hepatobiliary mode of action remain essential therapeutic agents for the treatment of cholestasis. They are denoted as cholagogues (promoting the flow of bile from the liver and gall bladder into the intestines) and choleretics (increasing bile production). Cholagogic and choleretic properties of rosemary (Rosmarinus officinalis) [1] and other medicinal plants have been confirmed in animal experiments and clinical studies.
Un système de détection de thématiques populaires sur Twitter

Twitter offre des fonctionnalités de microblogging qui sont utilisées par des millions de personnes à travers le monde pour publier des messages courts. Ces personnes créent et partagent de l’information liée à divers types d’évènements, allant d’évènements personnels banals à des évènements importants et/ou globaux, quasiment en temps-réel. L’explosion du nombre d’utilisateurs de ce service de réseautage social a entraîné l’apparition d’un phénomène de surcharge informationnelle. Pour lutter contre cela, il est nécessaire de doter les utilisateurs de moyens leur permettant d’identifier plus facilement les éléments d’information les plus intéressants et de se tenir au courant des derniers évènements significatifs. L’information brute produite par Twitter est délivrée sous la forme d’un flux de messages courts. Par conséquent la manière dont ceux-ci arrivent au fil du temps recèle une part importante de leur signification. La dynamique temporelle des thématiques les plus populaires sur ces réseaux est constituée d’une succession de focus et dé-focus, autrement dits, une succession de pics de popularité (Leskovec et al., 2009). C’est pourquoi de nombreuses approches – allant de méthodes basées sur la fréquence des mots (Benhardus et Kalita, 2013) jusqu’à des méthodes plus complexes reposant sur des modèles de thématiques probabilistes dynamiques (Lau et al., 2012) – ont été proposées dans le but d’identifier ce genre de thématiques. Dans cet article, nous présentons un système implémentant la méthode décrite par Guille et Favre (2014). Contrairement à la majorité des méthodes existantes, celle-ci prend en compte l’aspect social du flux traité en considérant la fréquence de création de liens dynamiques entre utilisateurs. Un utilisateur crée un lien dynamique en insérant une ou plusieurs mentions (i.e. « @pseudonyme ») dans un tweet. Ce lien entre utilisateurs est dynamique car lié au contenu du tweet et sa durée de vie. Par ailleurs, cette méthode localise plus précisément dans le temps les thématiques que les méthodes existantes et traite les grands volumes de données plus efficacement que celles à base de modèles de thématiques probabilistes. La suite de cet article est organisée comme suit. Dans la section 2 nous décrivons le fonctionnement du système puis dans la section 3 nous détaillons le cadre de la démonstration. Enfin dans la section 4 nous concluons.
HLA-E, HLA-F and HLA-G — The Non-Classical Side of the MHC Cluster

Traditionally, the MHC is divided into the classes containing groups of genes with related functions; the MHC class I and II genes encode for human leukocyte antigens (HLA), proteins that are displayed on the cell surface. In humans, MHC class I molecules com‐ prise the classical (class I-a) HLA-A, -B, and -C, and the non-classical (class I-b) HLA-E, F, -G and – H (HFE) molecules (Pietra et al., 2009). Both categories are similar in their mechanisms of peptide binding and presentation and in the induced T-cell responses (Rodgers and Cook, 2005). The most remarkable feature of MHC class I-b molecules is their highly conserved nature (van Hall et al., 2010). In contrast with class Ia molecules they have been under a distinct selective pressure, exhibiting very low levels of allelic polymor‐ phism (Strong et al., 2003). Classical MHC class I gene transcription is mediated by several cis-acting regulatory elements, in the proximal promoter region (Gobin and van den Elsen, 2000). Those elements determine the constitutive and cytokine induced expression levels of the molecule (Gobin and van den Elsen, 2000).
Practical radiation safety and protection for the endoscopist during ERCP.

Advances in radiology have allowed for an increasing array of diagnostic and therapeutic modalities in a wide range of medical disciplines. This has not only increased the use of radiographic equipment, but it has also increased the types of specialists who now incorporate radiology into their practice. In gastroenterology, endoscopists who perform ERCP make up one such group of nonradiologists, who are using radiation in the form of fluoroscopy as part of their practice. This increased use of fluoroscopy has increased their exposure to the risks associated with low-level radiation. Furthermore, some studies have shown that nonradiologists may receive higher doses during fluoroscopic procedures than their radiologist counterparts.1 The purpose of this article is to highlight some practical information regarding radiation biology for endoscopists and to outline some effective ways to decrease radiation exposure during fluoroscopic procedures.
E-BUSINESS AND THE DEATH OF FIRST MOVER ADVANTAGE

We have all heard about first mover advantage and how important it is, especially in the so-called "New Economy" and with e-related businesses. The story goes, “ If we are first in the market place, we can gain market recognition, market share early and the lion’s share of the market’s money. We can become entrenched in the market so that our lead will be insurmountable and then we can ride the wave of momentum and relax. " First mover advantage is a bit more complicated than that. In fact, being first may be a disadvantage. Ultimately, relying on being first mover as a(n) (Internet) strategy and/or competitive advantage involves a great amount of risk.
Toward a Tweet Typology: Contributory Consumer Engagement With Brand Messages by Content Type

ABSTRACT This study assesses brand messages on Twitter (i.e., tweets broadcasted by a brand) and the contributory engagement a tweet receives. It presents a typology for brand messages that accounts for 92.6% of messages found. Findings offer mild support for self-concept and self-enhancement as drivers of engagement. This research also tests assumptions made by marketers regarding social content. Brand messages that promoted giveaways positively influenced engagement, giving support to Berger's (2012) behavioral residue claim. Brand messages that mentioned popular culture events and current holidays positively influenced engagement, suggesting that brands that humanize do see benefits. Finally, promotional messages negatively influenced engagement, suggesting that consumers are skeptical of product information that comes directly from brands.
Analysis of Type I Error Rates of Univariate and Multivariate Procedures in Repeated Measures Designs

We compared the robustness of univariate and multivariate statistical procedures to control Type I error rates when the normality and homocedasticity assumptions were not fulfilled. The procedures we evaluated are the mixed model adjusted by means of the SAS Proc Mixed module, and Bootstrap-F approach, Brown–Forsythe multivariate approach, Welch–James multivariate approach, and Welch–James multivariate approach with robust estimators. The results suggest that the Kenward Roger, Brown–Forsythe, Welch–James, and Improved Generalized Aprroximate procedures satisfactorily kept Type I error rates within the nominal levels for both the main and interaction effects under most of the conditions assessed.
Multiple binding sites for polyomavirus large T antigen within regulatory sequences of polyomavirus DNA

Polyomavirus large T antigen binds specifically to multiple sites within the regulatory region of the viral genome. Experiments done with crude extracts from wild-type virus-infected mouse cells and immunoprecipitation of protein-DNA complexes localized two high-affinity binding sites on the early region side of the DNA replication origin. Purification of the large T antigen by immunoaffinity chromatography made it possible to refine the analysis through application of DNase I footprinting. The high-affinity interactions were resolved into three closely spaced, but distinct, binding regions. These begin at a site only slightly overlapping the early boundary of the core replication origin, a location highly homologous to that of simian virus 40 large T antigen-binding site I, but then extend away from the origin toward the early coding sequence and thus span the early region transcriptional initiation sites. Each tight-binding region contains from two to four copies of the sequence 5'-(A = T)G(A greater than G)GGC-3' repeated at 9- to 11-base-pair spacing. At high protein concentrations and at low ionic strength, additional sites within the core replication origin and in the enhancer region were protected from DNase I digestion. These minor binding sites also included repeats of sequences related to the consensus, but at different spacings. Our results suggest that, unlike simian virus 40 DNA, the polyomavirus genome may have distinct regions of interaction with its large T antigen which separately are involved in initiation of DNA replication and the regulation of viral transcription.
[A predictive model of fall prevention behaviors in postmenopausal women].

PURPOSE This study was done to propose and test a predictive model that would explain and predict fall prevention behaviors in postmenopausal women. The health belief model was the theoretical basis to aid development of a nursing intervention fall prevention program. METHODS Data for 421 postmenopausal women were selected from an original data set using a survey design. The structural equation model was tested for 3 constructs: modifying factors, expectation factors, and threat factors. Expectation factors were measured as relative perceived benefit (perceived benefit minus perceived barrier), self-efficacy, and health motivation; threat factors, as perceived susceptibility (fear of falling) and perceived severity (avoiding activity for fear of falling); and modifying factors: level of education and knowledge about fall prevention. Data were analyzed using SPSS Windows and AMOS program. RESULTS Mean age was 55.7 years (range 45-64), and 19.7% had experienced a fall within the past year. Fall prevention behaviors were explained by expectation and threat factors indicating significant direct effects. Mediating effect of health beliefs was significant in the relationship between modifying factors and fall prevention behaviors. The proposed model explained 33% of the variance. CONCLUSION Results indicate that fall prevention education should include knowledge, expectation, and threat factors based on health belief model.
Inconsistent requirements: an argumentation view

We present a logical framework for reasoning about inconsistent requirements in the context of the multi-viewpoint requirements engineering process. In order to analyse the sources of inconsistencies and to reason with inconsistent requirements, we present an argumentation view of the requirements. Intuitively, argumentation is a tool for reasoning with inconsistent knowledge: requirements are defined in terms of arguments (a conclusion with its support); then, a class of acceptable arguments is built (arguments with no counterarguments). We propose to characterize different classes of requirements which are ordered: from weakly confident to strongly confident (i.e. consistent). We present inference rules to build intra and inter-viewpoint reasoning. Inference rules are issued from the classes of requirements. We show how this work is useful for the requirements engineers to analyse inconsistent fragments of requirements.
Glioblastoma and Cerebral Microvascular Endothelial Cell Migration in Response to Tumor-associated Growth Factors

OBJECTIVEGlioma cell migration is determined by a complex interplay between soluble motogens and extracellular matrix components. Several growth factors are thought to be involved in glioma cell migration; however, little is known about their motogenic potency relative to one another. METHODSUsing modified Boyden chamber assays, we compared the chemotactic effects of scatter factor/hepatocyte growth factor (SF/HGF), transforming growth factor (TGF)-&agr;, TGF-&bgr;1, TGF-&bgr;2, epidermal growth factor (EGF), fibroblast growth factor (FGF)-1, FGF-2, insulin-like growth factor (IGF)-1, IGF-2, platelet-derived growth factor (PDGF)-AA, PDGF-BB, vascular endothelial growth factor (VEGF), pleiotrophin (PTN), and midkine (MK) in concentrations ranging from 1 pmol/L to 50 nmol/L on three different human glioblastoma cell lines. Checkerboard analyses distinguished between chemotaxis and chemokinesis. We further investigated the motogenic effects on human cerebral microvascular endothelial cells and analyzed receptor expression profiles. RESULTSSF/HGF was the most potent chemotactic factor for all three glioblastoma cell lines, inducing up to 33-fold stimulation of migration. TGF-&agr; showed the second strongest effect (up to 17-fold stimulation), and FGF-1 was also chemotactic for all three glioblastoma cell lines analyzed (maximal 4-fold effect). EGF, FGF-2, IGF-1, IGF-2, TGF-&bgr;1, and TGF-&bgr;2 were chemotactic for one or two of the cell lines (2- to 4-fold effects), whereas PDGF-AA, PDGF-BB, VEGF, PTN, and MK had no effect. In contrast, the most potent stimulators of cerebral microvascular endothelial cell migration were PDGF-AA (4-fold) and PDGF-BB (6-fold). CONCLUSIONThe expression levels of SF/HGF and TGF-&agr; as well as their respective receptors, MET and EGFR, are known to correlate with glioma malignancy grade. The particularly strong motogenic effects of these two growth factors suggest that they could be promising targets for an antimigratory component of glioma therapy, at least in comparison with the 12 other factors that were analyzed.
Self-aligned Schwann cell monolayers demonstrate an inherent ability to direct neurite outgrowth

In vivo nerve guidance channel studies have identified Schwann cell (SC) presence as an integral factor in axonal number and extension in an injury site, and in vitro studies have provided evidence that oriented SCs can direct neurite outgrowth. However, traditional methods used to create oriented SC monolayers (e.g. micropatterns/microtopography) potentially introduce secondary guidance cues to the neurons that are difficult to de-couple. Although SCs expanded on uniform laminin-coated coverslips lack a global orientation, the monolayers contain naturally formed regions of locally oriented cells that can be used to investigate SC-mediated neurite guidance. In this work, novel image analysis techniques have been developed to quantitatively assess local neurite orientation with respect to the underlying regional orientation of the Schwann cell monolayer. Results confirm that, in the absence of any secondary guidance cues, a positive correlation exists between neurite outgrowth and regional orientation of the SC monolayer. Thus, SCs alone possess an inherent ability to direct neurite outgrowth, and expansion of the co-culture-based quantitative method described can be used to further deconstruct specific biomolecular mechanisms of neurite guidance.
Decrypting the nexus between organisational culture, quality of work life, job satisfaction and employee productivity in the public sector

The recent recognition of the importance of the public sector as a major driver of economic success in any given country underlines the need to focus on strategies for enhancing the productivity of public sector employees. The aim of this paper is to examine the influence of organisational culture and quality of work life on job satisfaction and employee productivity among employees in the South African public sector. Using the quantitative survey research design, a questionnaire was developed and administered to a convenience sample of 264 employees drawn from central government departments in Gauteng. A confirmatory factor analysis was conducted to test the psychometric properties of measurement scales and hypotheses were tested using structural equation modelling. Organisational culture and quality of work life positively and significantly influenced job satisfaction. In turn, job satisfaction positively and significantly influenced employee productivity. Organisational culture exerted greater influence on job satisfaction than quality of work life. By implication, the labour relations environment in the public sector may be improved by nurturing healthy cultures and enriching quality of work life, thereby improving both job satisfaction and employee productivity.
Do Corporate Social Responsibility and Electronic Word-of-Mouth influence the Perceived Quality? Mediating role of Brand Image

The objective of this study is to examine the relationship between corporate social responsibilities, electronic words of mouth and perceived quality by considering the mediating role of brand image. For collecting data quantitative study was adopted, in which 450 questionnaire were distributed in various universities. Every student must belong to business department. Data has tested through SPSS (Statistical Packages for the Social Science) as well as AMOS (Analysis Movement of Structure). This study shows that there is positive and significant relationship between the corporate social responsibility and perceived quality and electronic word of mouth is highly correlated with perceived quality. Brand image also positively and significantly mediates the relationship among variables. Organizations must understand the importance of corporate social responsibility, electronic words of mouth and other significant factors and try to manage their brand image to influence the perceived quality. If the companies adopt CSR than the brand, image will be build up and in this way; the perceptions of the consumers will be influenced. Furthermore, companies can create awareness regarding their brand through the electronic word of mouth.
Fetal fibronectin detection for prediction of preterm birth in low risk women

(0.22 mm vs 0.40 mm; Braithwaite & Economides in press) and our conclusion was that TVS should be used in preference to TAS, especially in cases where the measurements are near the threshold level for karyotyping. We have assessed the training required before an individual is competent and have found that at least 30 to 40 and 40 to 50 supervised NT measurements are required with TAS and TVS, respectively, before operators achieve acceptable repeatable measurements. The reproducibility of NT measurements by Roberts et al. is poor, but we are unclear as to what kind of training in NT measurements the ultrasonographers had. We also found that N T measurements change with gestation and this should be taken into account when estimating risks for chromosomal abnormalities. It is important to derive reference ranges at given gestations and avoid the use of a single threshold value. In conclusion, NT measurement is possible in 100 % of fetuses between 9 and 13 weeks of gestation, and success rates and reproducibility can be improved using TVS. We agree that more studies are necessary before it is introduced as a screening programme in the general population.
Impact of Cultural Intelligence on Global Business

In today’s globalized business world, intercultural effectiveness is crucial to a firm’s survival. Cultural intelligence, CQ, is a four-dimensional construct that helps one to understand how the individual cultural beliefs and values influence motivations and behaviors (Ang & Van Dyne, 2009). CQ is related to the three aspects of intercultural effectiveness that include cultural judgment and decision making, cultural adaptation, and task performance (Ang et al., 2007). CQ plays an important role in the areas of global leadership (Van Dyne & Ang, 2006), achievement of managers (Rahimi et al., 2011), global strategic alliances, cross-cultural communications, negotiations, multinational teams (Early & Gibson, 2002), culturally diverse domestic teams, overseas work assignments (Bhaskar-Shrinivas, 2005; Lee & Sukco, 2010; Ramalu et al., 2012), global business competencies, and organizational effectiveness in the global marketplace (Creque, 2011). CQ is also relevant in establishing global identity in culturally diverse virtual teams (Adair et al., 2013).