Miracle-dz/UniFAR-FTUs huggingface dataset:
Polimorfisme Protein Darah Itik Pegagan dengan Metode PAGE

polymorphism of blood protein pegagan duck by PAGE method ABSTRACT . Pegagan duck is one of local duck species which comes from Ogan Ilir (OI) Regency, South Sumatera. This species is not popular, yet. But, it has spread out along Ogan river which comprises of three districts e.g Tanjung Raja, Inderalaya and Pemulutan. The ecosystem in this three districts is dominated by swampy area. As far, there is relatively limited information of Pegagan duck compared to another local duck. So, for the first step was done a reseach to identify genetic characteristic from blood protein analyses by electrophoresis method. It is expected that this information will become the bases in determining of the policy of Pegagan duck development. Polymorphism analyses of blood protein was done with 9 duck samples which taken randomly from 400 pegagan ducks. Protein locus observed were albumin (Alb), post albumin (Pa), transferrine (Tf), post transferrine-1 (Ptf-1), and post transferrine-2 (Ptf-2). Polymorphism analyses of blood protein of pegagan duck was characterized by allele A in locus Transferrine (TfA), Post transferrine-1 (Ptf-1A) and Post transferrine-2 (Ptf-2A) . Genetic diversity based on blood protein of Pegagan duck showed that gen frequency value in a range of 0,11 – 0,94 and high heterozygosis value that was 0,62 %.
Reaction of some selected soybean varieties (Glycine max (L) Merril) to root-knot nematode infection

Two-year screen-house studies to evaluate the reaction of five soybean varieties (TGX – 1987 – 34F, TGX -1987 – 38F, TGX – 1987 – 95F, TGX – 1986 -3F, TGX – 1985 – 8F) to root – knot nematode, Meloidogyne incognita , were conducted in the Faculty of Agriculture, University of Ilorin. Forty experimental pots containing pasteurized soil were used. Twenty pots were inoculated each with approximately 3,000 eggs of M. incognita while the other twenty which did not receive any treatment served as control. The experimental set up was a completely randomized design having each treatment replicated four times. Results showed that all the varieties were susceptible to M. incognita at varying degrees. While plant height of nematode inoculated varieties were significantly (P=0.05) lower than the un-inoculated plants, among the same varieties, there were significant differences in the number of branches of inoculated and non inoculated plants between different and among the same varieties. There were varietal differences in terms of seed weight which represented actual yield. Generally, the un-innoculated plants gave significantly higher yield than inoculated ones. Root gall infestation rated the same level for all the five varieties. However, variety TGX-1985 – 8F exhibited superior characteristics over the other four varieties because it showed higher level of tolerance to nematode infestation judging from its performance and yield. From the study, it can be deduced that the use of nematode resistant/tolerant Soybean varieties be adopted in the management of root- knot nematode infestation. It is one of the cheapest and safest control methods that pose no form of hazard to man and the environment. In nematode endemic ecological zones, TGX-1985 – 8F is therefore recommended as it proved to contain some specialized genes that conferred a higher level of tolerance against root- knot nematode, Meloidogyne incognita . Key Words : Glycine max, root – knot nematode, Dominant loci, Mi – 1.2, leucine zipper and R genes.
Isolation and characterization of neuronal substrates of the ubiquitin proteasome system

The covalent attachment of ubiquitin to proteins is one of the most common regulatory mechanisms in mammalian cells. It regulates numerous cellular processes through a combination of proteolytic and non-proteolytic forms of ubiquitination. In a neuronal context, the UPS has long been implicated in a variety of neurological disorders, and more recently, in normal neuronal function. Given the potential importance of the UPS in a neuronal framework, a logical step towards better understanding the scope of its function in neurons would involve an examination of the neuronal targets of the UPS. This research began with a large-scale identification of ubiquitinated, neuronal proteins. Using transgenic, His-6-ubiquitin tagged mice, in combination with denaturing nickel-affinity chromatography, we were able to specifically enrich for large amounts of ubiquitinated proteins. We identified over 385 unique proteins, of which 278 passed stringent filters of potential false-positives. With an approximate proteome of 1200 proteins, we concluded that at least 25% of all synaptic proteins were ubiquitinated at a detectable level. Following the screen, I conducted a more in-depth analysis of the more interesting targets. One of the more promising candidates identified was stromal interaction molecule I (STIM1). Characterization of neuronal STIM1 revealed that it was expressed throughout development with stable levels of expression in mature neurons. Furthermore, subpopulations of STIM1 were identified on the surface of hippocampal neurons. Ectopically expressed STIM1 rapidly redistributed into punctate clusters in response to thapsigargin (TG)-induced store-depletion. Proteasome inhibitors significantly increase surface STIM1 levels and peak Ca2+ influx upon TG -induced SOCE. Additionally, overexpression of POSH, an E3 ligase, leads to a decrease in STIM1 surface populations. Together, these results provide compelling evidence for previously undescribed roles of the ubiquitin-proteasome system (UPS) in the regulation of STIM1 and SOCE function. Examination of neuronal Rac1-GEF, kalirin-5, reveals that its stability is affected by CamKII and PP1/2 activity, in a proteasome-dependent manner. The role of kalirin-5 in the brain is yet unknown, but UPS-mediated regulation may provide a platform from which to pursue future studies
Evaluation of the central activity of the ethanolic extract of Acosmium subelegans (Mohlenbr) in mice

The ethanolic extract (EE) of Acosmium subelegans (Mohlenbr) Yakovl (perobinha-do-campo) was tested to behavioral paradigms in mice to investigate its putative central depressant effect. Oral pretreatment with the EE significantly reduced in a dose-dependent way the locomotor activity and increased by 30-55% the barbiturate sleep duration relatively to control values. At the highest dose (1,0 g.kg-1) it decreased the extension time/flexion time ratio of the maximal electroshockinduced convulsions, enhanced the latency to the pentylenetetrazol-induced convulsions and diminished by 26% the number of seizures, indicating an anticonvulsant action. No changes were observed in the motor coordination, the core temperature, climbing behavior, catalepsy and the plus-maze performance. The preliminary results indicate that the EE of A. subelegans induce a CNS depressant effect, more specifically an anticonvulsant effect that deserve a thorough investigation.
Efeitos do alumínio e do manganés no feijoeiro

Thirty eight bean ( Phaseolus vulgaris L.) cultivars were grown in nutrient solution under a factorial treatment using 4 levels of Al (0, 6, 12, 18 and 24 ppm) and 4 levels of Mn (0, 30, 60, 90 and 120 ppm) to study their behavior in presence of Al and Mn. By applying regression analysis to dry matter yield data it was possible to classify said varieties to Al effect in a. Tolerant – cultivars whose maximum yield appeared in the presence of the element at 1416 ppm: Jalo, Mulatinho Paulista, Ricobaio 1014 and Roxo 760; b. Moderately sensitive – cultivars whose minimum yield occurred in solutions having between 14 and 15 ppm of Al: Jamapa and Porrillo Sintetico; c. Sensitive – cultivars whose yield showed a minimum when the nutrient solution had between 2 and 9 ppm of Al: Carioca, Costa Rica, Costa Rica 1031, Cuva 168 N, Goiano Precoce, Rico Pardo, Rio Tibagi and Tamba. Mn toxicity regression analysis allowed the following classification: a. Moderately sensitive – cultivars showing minimum yield when the nutrient solution contained from 102 to 118 ppm of Mn: Carioca, CR 911, Cuva 168W, Rico Pardo and Rio Tibagi; b. Sensitive - cultivars showing minimum yield when the nutrient solution presented from 72 to 99 ppm of Mn: Costa Rica 1031, Goiano Precoce, Jamapa, Porrilio Sintetico and Rosinha. The remaining cultivars could not be classified because the estimated values of Al or Mn levels which would allow for maximum of minimum growth fell outside the experimental concentration (0 > Al > 24 and 0 > Mn > 120). For this reason it was not possible to draw safe conclusions on the data obtained.
Antiviral activity of azithromycin in cystic fibrosis airway epithelial cells

Virus-associated pulmonary exacerbations, most predominantly caused by rhinoviruses (RV), contribute to cystic fibrosis (CF) morbidity. However, there are only few therapeutic options to treat and/or prevent virus-induced CF pulmonary exacerbations. Recent evidence suggests that the macrolide antibiotic azithromycin (AZM) has antiviral properties in RV-infected primary human bronchial epithelial cells. We hypothesize that AZM induces antiviral mechanisms in primary CF bronchial epithelial cells. Primary bronchial epithelial cells from CF children were pretreated with AZM and infected with RV. Viral RNA, interferons (IFNs), IFN-stimulated genes (ISGs), RV pattern recognition receptors (PRRs) expressions were measured by RT-qPCR. Pro-inflammatory cytokine production was assessed by ELISA. Cell death was evaluated by Flow cytometry. After AZM pretreatment, RV load was 7-fold reduced in CF bronchial cells compared to untreated cells (median [interquartile range] 10 6 copies/reaction: 8.13 [5.28-34.4] vs. 56.8 [18.4-81.5]; p=0.001). The decreased RV load was not due to AZM-induced cytotoxicity in CF bronchial cells. Furthermore, AZM pretreatment significantly increased RV1B-induced IFNs and ISGs, and also PRRs mRNA expression in CF bronchial cells. Interestingly, while stimulating antiviral responses, AZM pretreatment did not significantly prevent virus-induced inflammatory cytokine production. AZM pretreatment reduces RV load in primary paediatric CF bronchial epithelial cells in vitro , possibly by amplifying the antiviral response mediated by the IFNs pathway. This study points to the potential of AZM as a novel therapeutic approach to treat and/or prevent RV-induced CF pulmonary exacerbations.
The time-frequency relationship between spot and futures prices of crude oil

This thesis investigates the relationship between daily spot and futures prices for maturities of one, two, three and four months of West Texas Intermediate (WTI) crude oil. The data cover period January 1987-April 2015. Based on economic theory, the futures prices should be closely related to the spot price, which in the case of crude oil market this thesis analyses using waveletbased approach. Main contributions of this thesis are findings in the field of time-frequency relationship of spot-futures prices of crude oil, where an alternative methodology wavelet transformation is used. The usage of this advanced method is also an additional contribution of this thesis because it allows us to rigorously study how co-movement (relationship) differs across frequencies/scales and time. In this thesis wavelet Coherence, wavelet bivariate correlation and relatively new method wavelet band spectral regression (WBLS) are used. This thesis brings 4 main findings. First, relationship between Futures and spot prices of crude oil is strong in all time-periods (frequencies/scales), which supports economic theory. Second and In contrary to the first finding, in the gasoline spot-futures market, we find that the relationship is strong mainly in higher scales (lower frequencies) while in lower scales (higher frequencies) the relationship is weak, so this goes against economic theory. Third, the crude oil relationship between forward prices and expected spot price are mainly in higher scales while in lower scales there is almost no relationship. Fourth, futures prices could be a good predictor of futures spot prices mainly in longterm period while in short-term futures prices seems to be biased predictor of futures spot prices due to existence of risk premia. JEL Classification C22, C32, C40, C58, E37, F30, G15
Medicinal plants of Azerbaijan flora used in the treatment of certain diseases

During investigations conducted within two years (2007 - 2009) 30 natural cenopopulations that were found, confirmed medicinal plants that can be used in the treatment of pediatric diseases. It was systematically studied life forms and distribution of 50 families, 94 genera and 97 species of medicinal plants widely spread in Azerbaijan flora which are used in the treatment of pediatric diseases. It was determined that the majority of medicinal plants contains grasses (71%, 69 species). Among them, perennials are characterized with the highest number (48 species, 49.5%), annuals with medium (12 species, 12.4%) and biennials with the least number (9 species, 9.3%). Profoundly described plant resources and role in the medicine of species such as, Berberis vulgaris, Crataegus pentagyna, Althaea officinalis, Hippophae rhamnoides, Inula helenium, Vaccinium myrtillis and Vaccinium vitis idaea detected and confirmed the area of distribution of medicinal plants, their vegetation phase and collected parts with priorities used in the treatment of pediatric diseases. Key words: Medical plants of Azerbaijan flora, systematic analysis, plant resources, pharmacological effect.
Song energetics of the bladder cicada, Cystosoma saundersii

1.Males of Cystosoma saundersii produced their calling songs while tethered to holders. 2.Oxygen consumption during singing was 0·136 ml min −1 , equivalent to 45·0 mW, an increase of 42·55 mW over the resting metabolic rate. 3.Maximum sound output was 90·6 dB at 20 cm. The whole sound-field was measured and represents a power output of 0·35 mW. The efficiency of sound production is 0·82%. 4.Heat production, estimated from the weight of singing muscle, was 28·7 mW. This leaves 13·5 mW of output unaccounted for. 5.Comparison with other species of insects suggests that none are more than an order of magnitude more efficient than C. saundersii .
Fertility status of selected rice soils in the Philippines.

Data from soil samples collected from 144 sites in the country were analyzed. The results showed that soil reaction varied from highly acidic to basic. Soil pH not only determines availability of nutrients but also excess of other substances such as sesquioxides, which are detrimental to plant growth under highly acidic soil conditions. Availability of P, for example, is minimized under both acidic and basic soil reactions. Zn deficiency is likely to occur at pH higher than 6.8. However results show that even at pH 6.0 and below there were already 45 sites where Zn was below critical level. This phenomenon can be also true with other nutrients. Only 2 sites were found to have sufficient N, P, K, Zn and S supplies, based on critical levels cited earlier. Majority of the sites (91%) were deficient in 2 or more nutrients. There were only 3 sites found to be limiting in N alone, 1 site each limiting in P and K, and 6 sites in Zn alone. These roughly constitute 8% of the total number of sites sampled. The multiple deficiencies of the samples were higher, ie, 30% of the sites were NPZn-deficient, 24% NPKZn-deficient, and 7% NPKZnS-deficient, comprising 42% of the total number of sites studied. The data shows that the observation that only N is the most limiting nutrient in lowland rice soils is no longer valid. The showed data that 91% of the sites studied showed 2 or more nutrients being limiting. The total area covered by the soil series planted to rice is about 1.2 million hectares. The total area which may be potentially deficient in essential nutrients could further be enlarged with the inclusion of Aklan, the Bicol Region, Cotabato and other rice growing regions not sampled in the present study. Among the soil series, San Manuel is the most widely distributed, and this series had shown multi-nutrient deficiency.
Approaching determination of H+-ATPase H+/ATP stoichiometry

The plant plasma membrane H+-ATPase was activated by the binding of a 14-3-3 protein. This complex can be stabilized by the fungal toxin - fusicoccin. During this activation H+/ATP stoichiometry changed (1). This work presents approaching to find the new stoichiometry. The main part presents looking for the best preparation of plasma membrane vesicles for measurements. A new pH indicator – Glu3 – was trapped inside vesicles in a few different ways such as Brij 58 treatment or sonication. Membranes were washed by ultracentrifugations or gel chromatography. The tight of prepared vesicles was examined by acid titration. The best result was achieved by sonication and gel chromatography. Brij 58 also affects the size of plasma membrane vesicles what was demonstrated. The average diameter decreases with increasing concentration of detergent.
Assessment of Hospital Physicians' Knowledge, Awareness, Attitude and Practice of Reporting Adverse Drug Reactions in Jeddah, Saudi Arabia

Background: Adverse drug reactions (ADRs) are an important cause of morbidity and mortality around the world. Spontaneous reporting of ADRs is considered an essential component of successful pharmacovigilance (PV). Physicians’ unfamiliarity with ADRs and reporting procedures are major factors that lead to sub-optimal reporting of ADRs. Objective: This study explored knowledge, awareness, attitude and practice (KAAP) of physicians towards ADRs and their reporting. Methods: This study was conducted at three general hospitals in Jeddah City. A 7-item, self-administered questionnaire was developed to explore physicians’ KAAP. Results: Majority of physicians had post-graduate qualification (n=243, 72%), were from medical departments (n=146, 43.3%), had long clinical experience (n=258, 77%) and consulted more than 10 patients daily (n=258, 77%). About 72% of physicians were never exposed to ADR training program. More than 60% of physicians were not fully aware of ADR reporting perspectives. Majority of them (75%) knew the correct definition of ADR and adequate knowledge of reportable ADRs (>90%). Majority of physicians depended on textbooks on drugs and therapies (31.2%) and drug package inserts (22.3%) as sources of ADR information. The majority of respondents (>90%) showed positive attitude towards ADRs and ADRs reporting and monitoring system. About 57.6% of physicians had come across ADRs in practice but only 21.7% reported these reactions. Most of physicians agreed to improve KAP towards ADRs reporting. Conclusion: The preliminary findings of this study suggest that though majority of physicians had good awareness and positive attitude towards ADR and ADR reporting, but needed correct knowledge in some areas of ADRs and their reporting system. Majority of physicians were not exposed to ADR training courses. Physicians certainly need ADR training programs in order to further enhance their KAAP towards ADRs and ADR reporting.
Basking Behavior of Emydid Turtles (Chysemys picta, Graptemys geographica, and Trachemys scripta) in an Urban Landscape

Abstract Basking is common in emydid turtles and is generally accepted to be a thermoregulatory behavior. In 2004, we quantified and described the basking behavior of turtles in the Central Canal of Indianapolis. This canal system flows through an urban landscape that is dominated by fragmented woodlots, residential areas, and commercial areas. We observed that basking turtles exhibited variable basking behavior, with spatial and temporal shifts in basking behavior from east-facing banks in the morning to west-facing banks in the afternoon. Turtles in the Central Canal are subject to frequent disturbance, which altered basking behavior. Many turtles forewent aerial basking on emergent substrates for aquatic basking on vegetation mats, which maintained warmer and more consistent temperatures than either emergent substrates or the surrounding water. Living in an intensively managed urban habitat, turtles in the Central Canal are susceptible to frequent anthropogenic perturbations, and future management should consider the life history and ecology of urban turtle populations
Microbial Evaluation and Antibiotic Susceptibility Profile of Isolates of Popular Sachet Water Brands Sold in Anambra State

the study and ABSTRACT Aims: To determine the microbiological quality of 5 brands of sachet water popularly consumed in Anambra state. Study Design: To validate the level of water treatment, and determine the type of bacteria and fungi contaminants, level of contamination and presence of resistant pathogens in the sachet water brands. Place and Duration of Study: Samples were collected based on the popularity and availability from different cities (Awka, Onitsha and Nnewi) in Anambra state. Analyses of the samples were conducted in the Department of Pharmaceutical Microbiology and Biotechnology, Nnamdi Azikiwe University, Awka, and National Food and Drug Administration and Control (NAFDAC), Nigeria. Both the sampling and analyses were done between April 2013 and May 2013. Methodology: 5 sachets of each of the sachet water brands were purchased from strategic locations in Anambra State and they were analysed using Heterotrophic Plate Count (HPC), total and fecal coliform count (TCC & FCC) and antibiotic susceptibility testing. Results: All the brands passed the HPC test. Brand 4 failed the TCC & FCC test, as the fecal coliform, E. coli was detected. Aspergillus niger was the most frequently encountered fungi with an occurrence rate of 100%. Staphylococcus aureus was the most frequently isolated bacteria with an occurrence rate of 60%. 53% of the isolated microorganisms are multi-drug resistant. Conclusion: Sachet water sold in Anambra state is of commendable microbial quality as four of the five brands passed the TCC & FCC test. However, some organisms isolated are multi-drug resistant and they can transfer their resistance to potentially virulent microbes.