Miracle-dz/UniFAR-FTUs huggingface dataset:
Generation and Applications of a DNA Aptamer against Gremlin-1

Gremlin-1, a highly conserved glycosylated and phosphorylated secretory protein, plays important roles in diverse biological processes including early embryonic development, fibrosis, tumorigenesis, and renal pathophysiology. Aptamers, which are RNA or DNA single-stranded oligonucleotides capable of binding specifically to different targets ranging from small organics to whole cells, have potential applications in targeted imaging, diagnosis and therapy. In this study, we obtained a DNA aptamer against Gremlin-1 (G-ap49) using in vitro Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Binding assay and dot-blot showed that G-ap49 had high affinity for Gremlin-1. Further experiments indicated that G-ap49 was quite stable in a cell culture system and could be used in South-Western blot analysis, enzyme-linked aptamer sorbent assay (ELASA), and aptamer-based cytochemistry and histochemistry staining to detect Gremlin-1. Moreover, our study demonstrated that G-ap49 is capable of revealing the subcellular localization of Gremlin-1. These data indicate that G-ap49 can be used as an alternative to antibodies in detecting Gremlin-1.
CSF opening pressure: Reference interval and the effect of body mass index

We prospectively recorded CSF opening pressure in 242 adults who had a lumbar puncture with concomitant measurement of weight and height. The 95% reference interval for lumbar CSF opening pressure was 10 to 25 cm CSF. Body mass index had a small but clinically insignificant influence on CSF opening pressure.
Evidence from the Agonadal Juvenile Male Rhesus Monkey (Macaca mulatta) for the View that the Action of Neurokinin B to Trigger Gonadotropin-Releasing Hormone Release Is Upstream from the Kisspeptin Receptor

Human genetics have revealed that kisspeptin signaling and neurokinin B (NKB) signaling are both required for robust pulsatile gonadotropin-releasing hormone (GnRH) release, and therefore for puberty and maintenance of adult gonadal function. How these two peptides interact to affect GnRH pulse generation remains a mystery. To address the hierarchy of the NKB and kisspeptin signaling pathways that are essential for GnRH release, two experiments were conducted using agonadal, juvenile male monkeys. Pituitary responsiveness to GnRH was first heightened by a pulsatile GnRH infusion to use the in situ pituitary as a bioassay for GnRH release. In the first experiment (n = 3), the kisspeptin receptor (KISS1R) was desensitized by a continuous 99-hour i.v. infusion of kisspeptin-10 (100 µg/h). During the last 4 h of continuous kisspeptin-10 infusion, desensitization of KISS1R was confirmed by failure of an i.v. bolus of kisspeptin-10 to elicit GnRH release. Desensitization of KISS1R was associated with a markedly blunted GnRH response to senktide. The response to senktide was progressively restored during the 72 h following termination of continuous kisspeptin-10. An analogous design was employed in the second experiment (n = 2) to desensitize the NKB receptor (neurokinin 3 receptor, NK3R) by administration of a continuous 48-hour i.v. infusion of senktide (200 µg/h). While a bolus of senktide during the last 3 h of continuous senktide administration failed to elicit GnRH release, thus confirming desensitization of NK3R, the ability of kisspeptin to stimulate GnRH was unimpaired. The foregoing findings support the view that NKB stimulation of GnRH release is upstream from KISS1R.
Picosecond Laser Pulse Distortion by Propagation through a Turbulent Atmosphere

We have been investigating the influence of atmospheric turbulence on the propagation of a picosecond laser pulse. The figure of merit of presented results is the time of propagation, its absolute delay and jitter. Phase wavefront deformation or beam profile changes were not studied. The correlation of the atmospheric turbulence with the propagation delay fluctuation was measured. The research was motivated by the needs of highly precise laser ranging of ground, air, and space objects; and highly precise and accurate time transfer ground-to-space and ground-to-ground by means of picosecond optical laser pulse. Firstly for comparison, lets briefly summarize the effects of a turbulent atmosphere to continuous laser beam. The total effect of atmospheric turbulences on a continuous laser beam propagation is a highly complex subject. Atmospheric turbulences can be defined as random spatial variations in the refraction index of the atmosphere resulting in a distortion of the spatial phase fronts of the propagating signal. Spatial phase front distortion induces the variable path of light energy and thus all effects described later on. Variations of the refraction index are caused by the turbulent motion of the atmosphere due to the variations in temperature and gradients in the water vapour. Following (Degnan, 1993), the optically turbulent atmosphere produces three effects on low power laser beams: 1) beam wander, 2) beam spread and 3) scintillations. Severe optical turbulence can result in a total beam breakup. Beam wander refers to the random translation of the spatial centroid of the beam and is generally caused by the larger turbulent eddies through which the beam passes. In astronomical community it is usually referred as seeing. Beam spread is a short term growth in the effective divergence of the beam produced by smaller eddies in the beam path. The two effects are often discussed together in terms of a “long term” and “short term” beam spread. The “long term” beam spread includes the effects of beam wander, whereas the “short term” beam spread does not. For more details, see (Degnan, 1993). Maximum turbulence occurs at mid-day in the desert (low moisture) under clear weather conditions. For the usual laser wavelength of 532 nm one can expect 2.4-4.6 cm for the coherence length at zenith angles of 0° and 70° respectively. At the tripled Nd:YAG wavelength (355 nm) the corresponding values are 3.1 and 1.6 cm (Degnan, 1993). Turbulence induced beam spreading will only have a significant impact on beam divergence (and hence signal level) if the coherence length is on the order of, or smaller than, the original effective beam waist radius. Since a typical 150 μrad beam implies an effective waist radius of 2.26 mm, the effect of beam spread on signal level for such systems is relatively small, i.e. a few percent.
Stability analysis for distributed perimeter controlled traffic networks

Perimeter control has shown great promise in mitigating congestion and managing heterogeneity in urban traffic networks. A highly heterogeneous traffic network can be partitioned into a great number of homogeneous regions, and distributed perimeter control structures are potentially useful due to fewer communication requirements. Disturbance propagation analysis is crucial in multi-region perimeter control, as a network instability issue arises when the disturbance is magnified while propagating through the regions. This paper establishes sufficient network stability conditions under which the disturbance is attenuated through propagation under a distributed control structure for a tree network. It is shown that these conditions have the same information structure as the distributed controller. Moreover, the number of these conditions grows linearly with the number of regions. Finally, the proposed conditions are verified via simulation.
Text detection in born-digital images by mass estimation

There is a need for effective web-document understanding due to the explosive progress of internet and network technologies. In this paper, we propose a new method for text detection in born-digital images by introducing a mass estimation concept. We propose to explore super-pixel information of different color channels to identify text atoms in images. The proposed method uses similarity graphs and spectral clustering to identify candidate text regions. We propose a new idea of mapping Gabor responses of a candidate text region to a spatial circle to study the spatial coherency of pixels. We introduce a mass estimation concept to identify text candidates from the pixel distribution in a spatial circle. The linear linkage graphs help in grouping text candidates to obtain full text lines. The same Gabor responses are used as features to eliminate false positives with an SVM classifier. We evaluate the proposed method for the testing on standard datasets, such as ICDAR 2013 (challenge-1) and the Situ et al. dataset. Experimental results on both the datasets show that the proposed method outperforms the existing methods.
An Architecture for Dynamically Interacting Agents

In this paper, we present the layered agent architecture INTERRAP which has been developed to cope with the basic requirements for Cooperative Intelligent Systems (CIS): agents shall behave in a situated, efficient, and goal-directed manner, and they shall be able to interact (i.e. coordinate and collaborate) with other agents. Over the past few years, agent architectures combining reactive with deliberative facilities have become very trendy in DAI. However, most attempts end up at the local planning layer and fail to take into account important mechanisms for interaction and collaboration among agents. INTERRAP extends previous attempts to build layered architectures by a cooperation component which holds cooperation knowledge as well as a library of joint plant. The modules of the model and the flow of control among them are explained. The model is described and evaluated by the FORKS application, the simulation of an automated loading-dock.
Important functional role of residue x of the presenilin GxGD protease active site motif for APP substrate cleavage specificity and substrate selectivity of γ‐secretase

γ‐Secretase plays a central role in the generation of the Alzheimer disease‐causing amyloid β‐peptide (Aβ) from the β‐amyloid precursor protein (APP) and is thus a major Alzheimer′s disease drug target. As several other γ‐secretase substrates including Notch1 and CD44 have crucial signaling functions, an understanding of the mechanism of substrate recognition and cleavage is key for the development of APP selective γ‐secretase‐targeting drugs. The γ‐secretase active site domain in its catalytic subunit presenilin (PS) 1 has been implicated in substrate recognition/docking and cleavage. Highly critical in this process is its GxGD active site motif, whose invariant glycine residues cannot be replaced without causing severe functional losses in substrate selection and/or cleavage efficiency. Here, we have investigated the contribution of the less well characterized residue x of the motif (L383 in PS1) to this function. Extensive mutational analysis showed that processing of APP was overall well‐tolerated over a wide range of hydrophobic and hydrophilic mutations. Interestingly, however, most L383 mutants gave rise to reduced levels of Aβ37–39 species, and several increased the pathogenic Aβ42/43 species. Several of the Aβ42/43‐increasing mutants severely impaired the cleavages of Notch1 and CD44 substrates, which were not affected by any other L383 mutation. Our data thus establish an important, but compared with the glycine residues of the motif, overall less critical functional role for L383. We suggest that L383 and the flanking glycine residues form a spatial arrangement in PS1 that is critical for docking and/or cleavage of different γ‐secretase substrates.
An introduction to reasoning over qualitative multi-attribute preferences

Abstract Research on preferences has significantly increased in recent years, as it involves not only many subproblems to be investigated, such as elicitation, representation, and reasoning, but has also been the target of different research areas, for example, artificial intelligence and databases. In particular, much work has focused on qualitative preferences, because these are closer to the way people express their preferences in comparison with quantitative preferences. Against this background, a large number of approaches have been proposed, associated with heterogeneous areas, so that these approaches are usually just compared with those of the same area. In response, we present in this paper a survey of approaches to qualitative multi-attribute preference reasoning, covering different research areas. We introduce selected approaches that propose different techniques and algorithms, which take as input qualitative multi-attribute preference statements following a particular structure specified by the approach. We analyse each approach in a systematic way and discuss their commonalities and limitations.
Transfer Learning for Speaker Verification on Short Utterances

Short utterance lacks enough discriminative information and its duration variation will propagate uncertainty into a probability linear discriminant analysis (PLDA) classifier. For speaker verification on short utterances, it can be considered as a domain with limited amount of long utterances. Therefore, transfer learning of PLDA can be adopted to learn discriminative information from other domain with a large amount of long utterances. In this paper, we explore the effectiveness of transfer learning based PLDA (TL-PLDA) on the NIST SRE and Switchboard (SWB) corpus. Experimental results showed that it could produce the largest gain of performance compared with the traditional PLDA, especially for short utterances with the duration of 5s and 10s.
Effectiveness of Mifepristone in the Treatment of Uterine Leiomyomata

Aims: The goal of this study was to determine the effects of mifepristone in perimenopausal women with leiomyomas, thereby to see a decrease in severity of symptoms and a decrease in size of the leiomyomas. Methods: This was a prospective study conducted at Eden HospitalMedical College and Hospital, Kolkatafrom July 2010 to June 2011. Fifty patients received 20-25mg mifepristone daily and comparison was made between pre and post treatment symptoms and leiomyoma volume. Results: All patients became amenorrhoeic after treatment, relieving heavy menstrual flow most complained of. Lower abdominal pain improved by > 80% in most patients and volume of leiomyoma decreased appreciably. The drug did not have any major side effects. Endometrial hyperplasia detected by ultrasound in 42% patients was only of simple type on biopsy. Conclusions: Mifepristone can be useful in treating symptomatic women with uterine leiomyoma in perimenopausal age group, in those awaiting surgery to stop bleeding and improve anaemia and to reduce size of tumor to make surgery technically easier, making it a cheaper alternative to GnRH agonists and without any major side effects. Nepal Journal of Obstetrics and Gynaecology / Vol 8 / No. 1 / Issue 15 / Jan- June, 2013 / 22-25 DOI: http://dx.doi. org/10.3126/njog.v8i1. 8856
Positive Youth Development: Thriving as the Basis of Personhood and Civil Society

Theoretical issues pertinent to a dynamic, developmental systems understanding of positive youth development and the thriving process in such development are discussed. Thriving involves relative plasticity in human development and adaptive regulations of person-context relations. An integrated moral and civic identity and a commitment to society beyond the limits of one's own existence enable thriving youth to be agents both in their own, healthy development and in the positive enhancement of other people and of society. Thriving youth become generative adults through the progressive enhancement of behaviors that are valued in their specific culture and that reflect the universal structural value of contributing to civil society.
Flexible and inflexible task sets: Asymmetric interference when switching between emotional expression, sex, and age classification of perceived faces

The present study investigated whether the processing characteristics of categorizing emotional facial expressions are different from those of categorizing facial age and sex information. Given that emotions change rapidly, it was hypothesized that processing facial expressions involves a more flexible task set that causes less between-task interference than the task sets involved in processing age or sex of a face. Participants switched between three tasks: categorizing a face as looking happy or angry (emotion task), young or old (age task), and male or female (sex task). Interference between tasks was measured by global interference and response interference. Both measures revealed patterns of asymmetric interference. Global between-task interference was reduced when a task was mixed with the emotion task. Response interference, as measured by congruency effects, was larger for the emotion task than for the nonemotional tasks. The results support the idea that processing emotional facial expression constitutes a more flexible task set that causes less interference (i.e., task-set “inertia”) than processing the age or sex of a face
Importance of the use of protocols for the management of analgesia and sedation in pediatric intensive care unit.

Introduction: Analgesia and sedation are essential elements in patient care in the intensive care unit (ICU), in order to promote the control of pain, anxiety and agitation, prevent the loss of devices, accidental extubation, and improve the synchrony of the patient with mechanical ventilation. However, excess of these medications leads to rise in morbidity and mortality. The ideal management will depend on the adoption of clinical and pharmacological measures, guided by scales and protocols. Objective: Literature review on the main aspects of analgesia and sedation, abstinence syndrome, and delirium in the pediatric intensive care unit, in order to show the importance of the use of protocols on the management of critically ill patients. Method: Articles published in the past 16 years on PubMed, Lilacs, and the Cochrane Library, with the terms analgesia, sedation, abstinence syndrome, mild sedation, daily interruption, and intensive care unit. Results: Seventy-six articles considered relevant were selected to describe the importance of using a protocol of sedation and analgesia. They recommended mild sedation and the use of assessment scales, daily interruptions, and spontaneous breathing test. These measures shorten the time of mechanical ventilation, as well as length of hospital stay, and help to control abstinence and delirium, without increasing the risk of morbidity and morbidity. Conclusion: Despite the lack of controlled and randomized clinical trials in the pediatric setting, the use of protocols, optimizing mild sedation, leads to decreased morbidity.
Residual Cell-Associated Unspliced HIV-1 Rna in Peripheral Blood of Patients on Potent Antiretroviral Therapy Represents Intracellular Transcripts

Unspliced HIV-RNA (HIV-UsRNA) associated with peripheral blood mononuclear cells (PBMCs) persists in patients on potent antiretroviral therapy even in the absence of detectable plasma HIV-RNA. To further characterize such residual HIV-RNA, cell-associated virion-encapsidated HIV and intracellular unspliced HIV-RNA were differentiated and monitored using a novel highly sensitive method. In addition, expression of HIV-mRNA encoding tat and rev was assessed. PBMCs of patients with unsuppressed plasma viraemia harboured an extracellular fraction of HIV-UsRNA, which correlated highly with intracellular HIV-RNA levels. Thus, extracellular PBMC-associated HIV-RNA may, to a significant extent, reflect nascent virions attached to productively infected cells. Upon treatment with potent antiretroviral therapy resulting in plasma viraemia <50 copies/ml, expression of cell-associated viral particles was hardly discernible in PBMCs but transcription of unspliced HIV-RNA persisted. Given the virtual absence of rev-mRNA, translation of residual HIV-UsRNA was probably precluded by retention of these transcripts in the nucleus. As shown by limiting dilution analysis, HIV-1 infected cells with such a repressed viral transcription pattern were observed at high frequencies in PBMC from untreated patients.
Impact du stress oxydant et de l’athérosclérose sur la fonction vasculaire cérébrale au cours du vieillissement

Reactive oxygen species can have different roles in neurons and cerebral vascular cells as low concentrations are beneficial while unlikable effects are observed at higher concentrations. Mice cerebral circulation is different from other vascular beds as hydrogen peroxide (H2O2) is a major endogenous endothelium-derived relaxing factor. The objective of our first study was to characterize the physiological implication of H2O2 derived from eNOS activation in mice cerebral arteries. We tried to identify the mechanisms implicated in flow-mediated dilation (FMD), the most physiological reactive endothelial function. Our study suggested that H2O2, but not nitric oxide, derived from cerebral eNOS activity was the main factor implicated in the regulation of FMD. However, the evolution of this dilatory pathway through ageing associated or not with risk factors for cardiovascular diseases is poorly understood. Ageing is associated with increase oxidative stress and endothelial dysfunction, the later characterized by an imbalance in the release of endothelial constricting and relaxing factors. Risk factors for cardiovascular diseases, such as hypercholesterolemia, can increase oxidative stress and could hasten endothelial dysfunction. However, the evolution of the endothelial factors through ageing, particularly H2O2 dilatory pathway, in the cerebral circulation is still not well described. In our second study, we hypothesise that alterations in endothelial factors might be responsible for (1) cerebral endothelial dysfunction observed during ageing and (2) the accelerated endothelial dysfunction associated with atherosclerosis. Our results suggested that increased in oxidative stress associated with ageing leads to the release of endogenous TXA2, which in turn, reduces eNOS activity and, consequently, reduces endothelial-dependent dilation. Furthermore, we found that oxidative stress increase associated with atherosclerosis hastens cerebral endothelial dysfunction in mice. The implication of oxidative stress was confirmed by the beneficial effect of the antioxidant catechin on atherosclerosis associated cerebral endothelial function. Finally, premature cerebral endothelial dysfunction observed during atherosclerosis could not only be associated with increase stroke incidence but also associated with a reduction in cerebral blood flow and, ultimately, a decrease in cognitive