Miracle-dz/UniFAR-FTUs huggingface dataset:
ACE2 and Furin Expressions in Oral Epithelial Cells Possibly Facilitate COVID-19 Infection via Respiratory and Fecal–Oral Routes

Background: Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that mainly transfers from human to human via respiratory and gastrointestinal routes. The S-glycoprotein in the virus is the key factor for the entry of SARS-CoV-2 into the cell, which contains two functional domains: S1 is an angiotensin-converting enzyme 2 (ACE2) receptor binding domain, and S2 is necessary for fusion of the coronavirus and cell membranes. Moreover, it has been reported that ACE2 is likely to be the receptor for SARS-CoV-2. In addition, mRNA level expression of Furin enzyme and ACE2 receptor had been reported in airway epithelia, cardiac tissue, and enteric canals. However, the expression patterns of ACE2 and Furin in different cell types of oral tissues are still unclear. Methods: In order to investigate the potential infective channel of the new coronavirus via the oropharyngeal cavity, we analyze the expression of ACE2 and Furin in human oral mucosa using the public single-cell sequence datasets. Furthermore, immunohistochemistry was performed in mucosal tissue from different oral anatomical sites to confirm the expression of ACE2 and Furin at the protein level. Results: The bioinformatics results indicated the differential expression of ACE2 and Furin on epithelial cells from different oral anatomical sites. Immunohistochemistry results revealed that both the ACE2-positive and Furin-positive cells in the target tissues were mainly positioned in the epithelial layers, partly expressed in fibroblasts, further confirming the bioinformatics results. Conclusions: Based on these findings, we speculated that SARS-CoV-2 could invade oral mucosal cells through two possible routes: binding to the ACE2 receptor and fusion with cell membrane activated by Furin protease. Our results indicated that oral mucosa tissues are susceptible to SARS-CoV-2 that could facilitate COVID-19 infection via respiratory and fecal–oral routes.
The Changing Landscape of Leadership Wisdom

Leadership wisdom lies in building enduring value for the firm. In a context of rapid technological change influencing the world of work, leaders need to focus on excellence in core aspects of production, sales and service. More than emotional forms of storytelling, excellence in core activities will help firms to build value for customers and themselves. At the same time, leaders need to build a balance between the value propositions of their firms and communities. They need to ensure that the changing world of work does not create unreasonable stress and precariousness for community actors.
Rumour Source Detection Using Game Theory

Social networks have become a critical part of our lives as they enable us to interact with a lot of people. These networks have become the main sources for creating, sharing and also extracting information regarding various subjects. But all this information may not be true and may contain a lot of unverified rumours that have the potential of spreading incorrect information to the masses, which may even lead to situations of widespread panic. Thus, it is of great importance to identify those nodes and edges that play a crucial role in a network in order to find the most influential sources of rumour spreading. Generally, the basic idea is to classify the nodes and edges in a network with the highest criticality. Most of the existing work regarding the same focuses on using simple centrality measures which focus on the individual contribution of a node in a network. Game-theoretic approaches such as Shapley Value (SV) algorithms suggest that individual marginal contribution should be measured for a given player as the weighted average marginal increase in the yield of any coalition that this player might join. For our experiment, we have played five SV-based games to find the top 10 most influential nodes on three network datasets (Enron, USAir97 and Les Misérables). We have compared our results to the ones obtained by using primitive centrality measures. Our results show that SV-based approach is better at understanding the marginal contribution, and therefore the actual influence, of each node to the entire network.
An Essential Requirement for Fgf10 in Pinna Extension Sheds Light on Auricle Defects in LADD Syndrome

The pinna (or auricle) is part of the external ear, acting to capture and funnel sound toward the middle ear. The pinna is defective in a number of craniofacial syndromes, including Lacrimo-auriculo-dento-digital (LADD) syndrome, which is caused by mutations in FGF10 or its receptor FGFR2b. Here we study pinna defects in the Fgf10 knockout mouse. We show that Fgf10 is expressed in both the muscles and forming cartilage of the developing external ear, with loss of signaling leading to a failure in the normal extension of the pinna over the ear canal. Conditional knockout of Fgf10 in the neural crest fails to recapitulate this phenotype, suggesting that the defect is due to loss of Fgf10 from the muscles, or that this source of Fgf10 can compensate for loss in the forming cartilage. The defect in the Fgf10 null mouse is driven by a reduction in proliferation, rather than an increase in cell death, which can be partially phenocopied by inhibiting cell proliferation in explant culture. Overall, we highlight the mechanisms that could lead to the phenotype observed in LADD syndrome patients and potentially explain the formation of similar low-set and cup shaped ears observed in other syndromes.
Mechano-Electric Coupling and Arrhythmogenic Current Generation in a Computational Model of Coupled Myocytes

A wide range of arrhythmogenic phenotypes have been associated with heterogeneous mechanical dyskinesis. Pro-arrhythmic effects are often associated with dysregulated intra-cellular calcium handling, especially via the development of intra- and inter-cellular calcium waves. Experimental evidence suggests that mechanical strain can contribute to the generation and maintenance of these calcium waves via a variety of mechano-electric coupling mechanisms. Most model studies of mechano-electric coupling mechanisms have been focused on mechano-sensitive ion channels, even though experimental studies have shown that intra- and inter-cellular calcium waves triggered by mechanical perturbations are likely to be more prevalent pro-arrhythmic mechanisms in the diseased heart. A one-dimensional strongly coupled computational model of electromechanics in rabbit ventricular cardiomyocytes showed that specific myocyte stretch sequences can modulate the susceptibility threshold for delayed after-depolarizations. In simulations of mechanically-triggered calcium waves in cardiomyocytes coupled to fibroblasts, susceptibility to calcium wave propagation was reduced as the current through the gap junction caused current drain from the myocytes. In 1D multi-cellular arrays coupled via gap junctions, mechanically-induced waves may contribute to synchronizing arrhythmogenic calcium waves and after-depolarizations.
Nitrogen Uptake by Two Plants in Response to Plant Competition as Regulated by Neighbor Density

Plant species may acquire different forms of nitrogen (N) to reduce competition for the same resource, but how plants respond to neighbors with different densities in their N uptake is still poorly understood. We investigated the effects of competition regime on the uptake of different N forms by two hygrophytes, Carex thunbergii and Polygonum criopolitanum, by conducting a hydroponic test of excised roots and an in situ experiment in a subtropical wetland ecosystem. The two species were grown either in monocultures or mixtures with various neighbor densities. Root functional traits and N uptake rates of different N forms were measured. Our results showed that N uptake was mainly determined by N form, rather than species identity. Both species were able to use organic N sources, but they took up relatively more N supplied as NO3- than as NH4+ or glycine, irrespective of competition treatments. Both species preferred NO3- when grown in monoculture, but in the presence of competitors, the preference of fast-growing C. thunbergii persisted while P. criopolitanum acquired more NH4+ and glycine, with stronger responses being observed at the highest neighbor density. The hydroponic test suggested that these divergences in N acquisition between two species might be partially explained by different root functional traits. To be specific, N uptake rates were significantly positively correlated with root N concentration and specific root length, but negatively correlated with root dry matter content. Our results implicated that C. thunbergii has a competitive advantage with relatively more stable N acquisition strategy despite a lower N recovery than P. criopolitanum, whereas P. criopolitanum could avoid competition with C. thunbergii via a better access to organic N sources, partly mediated by competition regimes.
Isolation of Burkholderia sp. HQB-1, A Promising Biocontrol Bacteria to Protect Banana Against Fusarium Wilt Through Phenazine-1-Carboxylic Acid Secretion

Fusarium wilt is a devastating soil-borne fungal disease caused by Fusarium oxysporum f.sp. cubense (Foc). In recent years, some antifungal bacteria have been applied for the prevention and biocontrol of pathogenic fungi. In our study, a bacterial strain HQB-1, isolated from banana rhizosphere soil, was cultured for investigation. It showed broad-spectrum antifungal activities against representative phytopathogenic fungi including Fusarium oxysporum, Colletotrichum gloeosporioides, Botrytis cinerea, and Curvularia fallax. The strain HQB-1 was identified as Burkholderia sp. by morphological, physiological, and biochemical examinations, confirmed by 16S rRNA gene sequence analysis. Among the metabolites produced by the strain, we identified an antifungal compound which was identified phenazine-1-carboxylic acid (PCA) (C13H8N2O2) through ultraviolet, liquid chromatography quadrupole-time of flight mass spectrometer, and nuclear magnetic response. Furthermore, PCA exhibited the lowest minimum inhibitory concentration (MIC) against F. oxysporum (1.56 μg/ml) and yielded the highest MIC against C. gloeosporioides. Pot experiments showed that application of 5 μg/ml or more of PCA efficiently controlled banana wilt and promoted the growth of banana plants. These results suggested that Burkholderia sp. HQB-1, as an important microbial resource of PCA, could be a promising biological agent against wilt diseases and promoting banana growth.
The Utility of Diffusion-Weighted MRI Lesions to Compare the Effects of Different Heparinization Schemes in Intracranial Aneurysms Treated by Endovascular Intervention

Objective: Heparinization is applied to prevent ischemic complications in the endovascular treatment of intracranial aneurysms, but there is no unified heparinization scheme. Diffusion-weighted imaging (DWI) can be used to evaluate ischemia after endovascular therapy for intracranial aneurysms. The goal of this study is to apply DWI to evaluate the effects of different heparinization schemes on intracranial aneurysms treated with endovascular therapy. Methods: We retrospectively reviewed 141 patients with 149 aneurysms treated with endovascular interventions from July 2019 to April 2020 at our center, including 96 aneurysms treated with local heparinization and 53 aneurysms treated with systemic heparinization. We collected the basic information of the patients, including age, sex, comorbidities, and aneurysm characteristics, and associated treatment data. New ischemic lesions detected by DWI were categorized belonging to four types. Multivariate logistic regression was used to compare the effects of different heparinization schemes on intracranial aneurysms treated with endovascular therapy. Results: There were no significant differences in age, sex, hypertension, diabetes, and aneurysm size or location between the two groups. The incidence and distribution types of DWI abnormalities in the local heparinization groups and systemic heparinization groups were not significantly different (P > 0.05). There was a correlation between the laser engraving stent and postoperative DWI abnormalities (P < 0.003). Multivariate logistic regression analysis showed that the laser engraving stent was significantly correlated with postoperative DWI abnormalities (odds ratio, 4.71; 95% CI: 1.51–14.58; P = 0.007). Conclusion: Compared with systemic heparinization, local heparinization does not increase the incidence of DWI abnormalities after endovascular treatment, and its application in this group of patients is safe and effective.
Hybrid Incompatibilities and Transgressive Gene Expression Between Two Closely Related Subspecies of Drosophila

Genome-wide assays of expression between species and their hybrids have identified genes that become either over- or underexpressed relative to the parental species (i.e., transgressive). Transgressive expression in hybrids is of interest because it highlights possible changes in gene regulation linked to hybrid dysfunction. Previous studies in Drosophila that used long-diverged species pairs with complete or nearly complete isolation (i.e., full sterility and partial inviability of hybrids) and high-levels of genome misregulation have found correlations between expression and coding sequence divergence. The work highlighted the possible effects of directional selection driving sequence divergence and transgressive expression. Whether the same is true for taxa at early stages of divergence that have only achieved partial isolation remains untested. Here, we reanalyze previously published genome expression data and available genome sequence reads from a pair of partially isolated subspecies of Drosophila to compare expression and sequence divergence. We find a significant correlation in rates of expression and sequence evolution, but no support for directional selection driving transgressive expression in hybrids. We find that most transgressive genes in hybrids show no differential expression between parental subspecies and used SNP data to explore the role of stabilizing selection through compensatory mutations. We also examine possible misregulation through cascade effects that could be driven by interacting gene networks or co-option of off-target cis-regulatory elements.
Gesture Influences Resolution of Ambiguous Statements of Neutral and Moral Preferences

When faced with an ambiguous pronoun, comprehenders use both multimodal cues (e.g., gestures) and linguistic cues to identify the antecedent. While research has shown that gestures facilitate language comprehension, improve reference tracking, and influence the interpretation of ambiguous pronouns, literature on reference resolution suggests that a wide set of linguistic constraints influences the successful resolution of ambiguous pronouns and that linguistic cues are more powerful than some multimodal cues. To address the outstanding question of the importance of gesture as a cue in reference resolution relative to cues in the speech signal, we have previously investigated the comprehension of contrastive gestures that indexed abstract referents – in this case expressions of personal preference – and found that such gestures did facilitate the resolution of ambiguous statements of preference. In this study, we extend this work to investigate whether the effect of gesture on resolution is diminished when the gesture indexes a statement that is less likely to be interpreted as the correct referent. Participants watched videos in which a speaker contrasted two ideas that were either neutral (e.g., whether to take the train to a ballgame or drive) or moral (e.g., human cloning is (un)acceptable). A gesture to the left or right side co-occurred with speech expressing each position. In gesture-disambiguating trials, an ambiguous phrase (e.g., I agree with that, where that is ambiguous) was accompanied by a gesture to one side or the other. In gesture non-disambiguating trials, no third gesture occurred with the ambiguous phrase. Participants were more likely to choose the idea accompanied by gesture as the stimulus speaker’s preference. We found no effect of scenario type. Regardless of whether the linguistic cue expressed a view that was morally charged or neutral, observers used gesture to understand the speaker’s opinion. This finding contributes to our understanding of the strength and range of cues, both linguistic and multimodal, that listeners use to resolve ambiguous references.
Investigation of the Cold-Start Combustion Characteristics of Ethanol−Gasoline Blends in a Constant-Volume Chamber

This experimental study conducted in a closed combustion chamber is to investigate combustion characteristics of ethanol−gasoline blends at low temperature, which is related to the cold-start operation of engines fueled with ethanol−gasoline. It presents the effects of the fuel amount injected into the combustion vessel on the combustion process, and some key characteristic parameters, such as ignition delay time, mass burning rate, and flame speeds, have been explored. The result shows that, for an ethanol−gasoline engine, it must not be overfueled to realize a reliable cold start, as is the case for a gasoline engine at the same temperature, especially at a temperature range around ethanol's boiling point, because ethanol addition into gasoline results in the improvement of blend evaporation. Moreover, The exhaust emissions are purposely measured in terms of unburned hydrocarbon (HC), carbon monoxide (CO), and oxides of nitrogen (NOx) emissions. It is confirmed that the emissions of HC during rich combu.
Flexible and Autonomous Integrated System for Characterizing Metal Oxide Gas Sensor Response in Dynamic Environment

Characterization and calibration of gas sensor is a complex problem due to the dynamic behavior of gases and the limitations of current technology. This article reports a flexible, robust, and autonomous integrated system that is able to perform characterization on metal oxide-based gas sensors in dynamic environments. The system controls the concentration and flow of the relevant gases into the gas chamber and simultaneously measuring the sensor response. This feature allows the characterization of the sensor under continuous dynamic flow of gases similar to conditions on a robot or flow pipes. Several experiments have been performed on the system using hydrogen sulfide. The results provide information on the general characteristics of the sensor as well as its sensitivity. The noise levels were studied with different reference voltages. Overall, the results verify that the system is reliable and able to produce repeatable measurements.
Shoulder Pain in a Weight-lifting Adolescent Athlete: A Zoonotic Etiology

Introduction More than 50%of high school students participate in a sport (1), which increases their risk of injury. Musculoskeletal pain is the second most common complaint in the adolescent athlete (2). It is more likely to occur in training or competition (63.8%), usually from indirect or overuse causes (68.8%). This can cause discontinuation of the sport (3). It has been reported that 43.5%of adolescent athletes currently complain of pain and 54.3% have claimed it within the last year (3). Skeletal immaturity increases susceptibility to pain and injury from lack of rest, training overload, and poor technique (2,3). Maladaptive bone and soft tissue changes from pain affect function and increase risk for further injury, correlating to longer recovery times. There are nearly a million estimated weight training injury visits per year in emergency departments, with up to 75% being acute injuries (4). Shoulder injuries are the fifth most common injury and make up 8% of all injuries sustained by high school athletes (1). Resistance injuries, in which free weights account for 90%, can occur from technical errors (31%), fatigue and overloading (81%), and dropping weights (65%) (4). The lift phase of the bench press causes 47% to 70% of these injuries. The pectoralis major-powered forced abduction of the arm puts the trainee at risk for pectoralis tendon ruptures, proximal biceps tendon ruptures, capsulolabral injuries, and other shoulder pain (4). Long-term physical consequences of these injuries are instability and joint degeneration (1). Recovery, however, involves an emotional meshwork of complex psychosocial factors, such as fear avoidance, catastrophic thinking, maladaptive coping, and avoidance behaviors that all prolong recovery (5). Determining appropriate diagnosis decreases these issues and aids in recovery. To aid in determining appropriate diagnosis, a systematic approach to shoulder pain is essential. Initially, it is important
Effects of N-acetylcysteine treatment on ethanol's rewarding properties and dopaminergic alterations in mesocorticolimbic and nigrostriatal pathways.

Recent reports have shown that N-acetylcysteine (N-AC) has beneficial effects in the treatment of cocaine and nicotine abuse. Considering the similar neurobiologic mechanisms involved in the development of addiction to different drugs, N-AC treatment could be useful in the treatment of ethanol abuse. The rewarding properties of the drugs of abuse plays an important role in the development of addiction and can be studied using the conditioned place preference (CPP) paradigm. Thus, to study the effects of N-AC treatment in the rewarding effects of ethanol, we investigated the effects of N-AC administration in the ethanol-induced CPP and neurochemical alterations within the mesocorticolimbic and the nigrostriatal dopaminergic pathways. Adult male Swiss mice were pretreated with N-AC (60 or 120 mg/kg intraperitoneal) and tested for the development, expression, or extinction of the ethanol-induced CPP. Another cohort of animals received N-AC (60 or 120 mg/kg intraperitoneal) 2-h before an acute administration of ethanol and had their brains removed for dopamine and its metabolites quantification in the mesocorticolimbic and nigrostriatal pathways. Pretreatment with N-AC (120 mg/kg) blocked the development of ethanol-induced CPP. On the other hand, N-AC at both doses did not alter the expression nor the extinction of ethanol-induced CPP. N-AC increased 3,4-dihydroxyphenylacetic acid content in the medial prefrontal cortex and dopaminergic turnover within the substantia nigra. Besides that, there was an increase in dopamine content in the nucleus accumbens of ethanol-treated animals. In summary, N-AC treatment blocked the development of ethanol CPP, without altering ethanol effects on dopaminergic neurotransmission.
Enhancing the Low Oral Bioavailability of Sulpiride via Fast Orally Disintegrating Tablets: Formulation, Optimization and In Vivo Characterization

Sulpiride (SUL) is a dopamine D2-receptor antagonist used for management of GIT disturbance and it has anti-psychotic activities based on the administered dose. SUL undergoes P-glycoprotein efflux, which lead to poor bioavailability and erratic absorption. Therefore, the objective of this research was an attempt to enhance the oral bioavailability of SUL via formulation of fast disintegrating tablets (SUL-FDTs) with a rapid onset of action. A 32 full-factorial design was performed for optimization of SUL-FDTs using desirability function. The concentration of superdisintegrant (X1) and Prosolv® (X2) were selected as independent formulation variables for the preparation and optimization of SUL-FDTs using direct compression technique. The prepared SUL-FDTs were investigated regarding their mechanical strength, disintegration time, drug release and in vivo pharmacokinetic analysis in rabbits. The optimized formulation has hardness of 4.58 ± 0.52 KP, friability of 0.73 ± 0.158%, disintegration time of 37.5 ± 1.87 s and drug release of 100.51 ± 1.34% after 30 min. In addition, the optimized SUL-FDTs showed a significant (p < 0.01) increase in Cmax and AUC(0–∞) and a relative bioavailability of about 9.3 fold compared to the commercial product. It could be concluded that SUL-FDTs are a promising formulation for enhancing the oral bioavailability of SUL concomitant with a fast action.