Miracle-dz/UniFAR-FTUs huggingface dataset:
Induction of degenerative brain lesions after adoptive transfer of brain lymphocytes from Borna disease virus-infected rats: presence of CD8+ T cells and perforin mRNA

Lymphocytes were isolated from the brains of Borna disease virus-infected donor Lewis rats at various time points after infection. Cell populations were characterized by cytofluorometry, with special emphasis on CD4+ and CD8+ cells. Testing of isolated lymphocytes revealed major histocompatibility complex class I-restricted cytotoxic activity. Reverse transcription-PCR analyses of brain homogenates of infected donors revealed the presence of CD8 mRNA after day 11 of infection and of perforin mRNA between days 13 and 25 after infection. Adoptive transfers of lymphocytes isolated from the brain at days 13 and 21 resulted in severe neurological symptoms, resembling experimental Borna disease. The onset of disease was dependent on the cell numbers transferred and was clearly related to the appearance of T cells in the brain. CD8+ T cells were found in the parenchyma, whereas CD4+ T cells were found predominantly in perivascular locations. A disseminated lymphocytic infiltration in the parenchyma was accompanied by severe morphological alterations, including significant necrosis of neurons. Furthermore, a prominent spongiform-like degeneration was observed; this increased over time and finally resulted in severe cortical brain atrophy. Lymphocytes obtained during the beginning chronic phase of experimental Borna disease in rats had no significant cytolytic capacity in vitro and were also not able to induce neurological symptoms typical of Borna disease after adoptive transfer. The data presented here show for the first time that lymphocytes isolated from the site of the inflammatory lesions, namely, the brains of diseased rats, induce the immunopathological reaction and cause Borna disease. After transfer, the pathological alterations induced in the recipients exactly reflect those observed during experimentally induced Borna disease in rats, including necrosis of neurons and glial cells and gross degeneration resulting in cortical brain atrophy. Evidence that the immunopathology of Borna disease is closely related to the presence of CD8+ T cells in the brain parenchyma is provided.
Randomized, double-blind, placebo-controlled, pilot trial of high-dose methylprednisolone in aneurysmal subarachnoid hemorrhage.

OBJECT The object of this study was to determine the efficacy of methylprednisolone in reducing symptomatic vasospasm and poor outcomes after subarachnoid hemorrhage (SAH). METHODS Ninety-five patients with proven SAH were recruited into a double-blind, placebo-controlled, randomized trial. Starting within 6 hours after angiographic diagnosis of aneurysm rupture, placebo or methylprednisolone, 16 mg/kg, was administered intravenously every day for 3 days to 46 and 49 patients, respectively. Deterioration, defined as development of a focal sign or decrease of more than 1 point on the Glasgow Coma Scale for more than 6 hours, was investigated by using clinical criteria and transcranial Doppler ultrasonography, cerebral angiography, or CT when appropriate. The end points were incidence of symptomatic vasospasm (delayed ischemic neurological deficits associated with angiographic arterial narrowing or accelerated flow on Doppler ultrasonography, or both) and outcome 1 year after entry into the study according to a simplified Rankin scale (Functional Outcome Scale [FOS]) in living patients and the Glasgow Outcome Scale in all patients included. RESULTS All episodes of deterioration and all living patients with a 1-year outcome were assessed by a review committee. In patients treated with methylprednisolone, the incidence of symptomatic vasospasm was 26.5% compared with 26.0% in those given placebo. Poor outcomes according to FOS were significantly reduced in the Methylprednisolone Group at 1 year of follow-up; the risk difference was 19.3% (95% CI 0.5-37.9%). The outcome was poor in 15% (6/40) of patients in the Methylprednisolone Group versus 34% (13/38) in the Placebo Group. CONCLUSIONS A safe and simple treatment with methylprednisolone did not reduce the incidence of symptomatic vasospasm but improved ability and functional outcome at 1 year after SAH.
Substrate integrated waveguide nShaped slot antenna for 57–64GHz band applications

Feature Scenario of Substrate integrated waveguide (SIW) is a good solution for implementing centimeter and millimeter wave applications. In this paper, presented SIW π slot antenna for 57–64 GHz band applications which is designed by using Rogers RT/Duriod 5880 whose dielectric constant is 2.2 and thickness of 0.381mm. Computer simulation technology studio suite is used for simulation and Observed the results reflection coefficient, VSWR, gain, radiation pattern. Obtained 3.28GHz bandwidth with respect to −10 dB reference line with two resonant frequency 60GHz and 62 GHz and return loss are −37.588dB, −33.05dB. Radiation efficiency of proposed antenna is 92.5% at 60GHz and 95.5% at 62GHz.
IKBKE promotes glioblastoma progression by establishing the regulatory feedback loop of IKBKE/YAP1/miR-Let-7b/i

Recently, we have demonstrated that IKBKE (inhibitor of nuclear factor kappa-B kinase subunit epsilon) is overexpressed in human glioblastoma and that inhibition of IKBKE remarkably suppresses the proliferative and invasive behaviour of glioblastoma cells. However, the specific pathogenic molecular mechanism remains to be elucidated. In this study, we verified that IKBKE promotes YAP1 expression via posttranslational modification and accelerates YAP1 translocation to the nucleus for the development of glioblastoma. We then determined that YAP1 negatively regulates miR-let-7b/i by overexpressing and silencing YAP1 expression. In addition, miR-let-7b/i feedback decreases the expression of IKBKE and YAP1 and suppresses the transportation of YAP1 located in the nucleus. Therefore, the regulatory feedback circuit of IKBKE↑→YAP1↑→miR-let-7b/i↓→IKBKE↑ dictates glioblastoma progression. Thus, we propose that blocking the circuit may be a new therapeutic strategy for the treatment of glioblastoma.
The influence of sensationalist features in television news stories on perceived news quality and perceived sensationalism of viewers in different age groups.

This study investigates whether the decreased trust in news media can be explained by the increase in sensationalism in news. To this end, an experiment was conducted in which viewers (N = 288) evaluated sensationalist versus non-sensationalist television news in terms of perceived news quality and perceived sensationalism. Each participant watched four television news stories that varied in the presence of two categories of sensationalism: arousing content and tabloid packaging. Findings showed that sensationalist television news tends to be more negatively evaluated than non-sensationalist news. In addition, critical views on arousing content appeared to be particularly visible among young and middle-aged adults. These findings suggest that the rise of sensationalist news could be an explanation of the declining trust in news media that is witnessed in a number of countries.
Teaching page replacement algorithms with a Java-based VM simulator

Computer system courses have long benefited from simulators in conveying important concepts to students. We have modified the Java source code of the MOSS virtual memory simulator to allow users to easily switch between different page replacement algorithms including FIFO, LRU, and Optimal replacement algorithms. The simulator clearly demonstrates the behavior of the page replacement algorithms in a virtual memory system, and provides a convenient way to illustrate page faults and their corresponding page fault costs. Equipped with a GUI for control and page table visualization, it allows the student to visually see how page tables operate and which pages page replacement algorithms evict in case of a page fault. Moreover, class projects may be assigned requiring operating system students to code new page replacement algorithms which they want to simulate and integrate them into the MOSS VM simulator code files thus enhancing the students' Java coding skills. By running various simulations, students can collect page replacement statistics thus comparing the performance of various replacement algorithms.
Disclosing complementary and alternative medicine use in the medical encounter: a qualitative study in women with breast cancer.

BACKGROUND Despite recent findings that patients who use complementary and alternative medicine (CAM) typically choose not to mention this to their physicians, little is known about the reasons for this lack of communication. Understanding the reasons for nondisclosure of CAM use is critical to improving physician-patient communication and patient care. METHODS We are conducting a 5-year prospective cohort study consisting of 4 interview cycles. The multiethnic, population-based sample consists of 86 San Francisco residents with recently diagnosed breast cancer (response rate = 87%). Findings are determined using qualitative analysis of transcribed interviews. RESULTS At initial contact, 72% of the participants were using at least 1 CAM therapy for breast cancer. Six months later, 65% of participants were using CAM. Of the women being treated by an alternative practitioner, 54% disclosed their CAM use to their physicians. Conversely, 94% discussed details of their biomedical treatments with their alternative practitioner. Reasons for not disclosing CAM use included anticipating the physician's disinterest, negative response, or unwillingness or inability to contribute useful information; the perception that the CAM therapies used were irrelevant to the biomedical treatment course; and the patients' views regarding the appropriate coordination of disparate healing strategies. CONCLUSIONS Discussions of patients' CAM use are more poorly integrated into the medical encounter than discussions of biomedical treatment are with alternative practitioners. Patients' disclosure is often cautiously modulated, even by those who would welcome an open discussion with their physicians. Specific suggestions for discussing CAM use with patients are presented.
Combat stressors and post-traumatic stress in deployed military healthcare professionals: an integrative review.

BACKGROUND While there has been a growing body of literature on the impact of combat stressors and post-traumatic stress on military service members involved in current conflicts, there has been little available information that directly examines the impact of these on healthcare providers. AIMS Aims for this integrative review included: (1) identifying exposures, experiences and other factors influencing stress responses in military healthcare providers previously engaged in a war effort and (2) describing the incidence of post-traumatic stress and related mental health problems in this population. REVIEW METHODS Using Cooper's integrative review method, relevant documents were collected and analysed using content categories and a coding scheme to assist with identifying and recording data for units of analysis. DATA SOURCES Literature searches (including all years to present) were conducted using keywords for stress reaction, for healthcare provider and for military war effort involvement. Literature was obtained using the Cumulative Index to Nursing and Allied Health Literature, the National Library of Medicine and the American Psychological Association databases. RESULTS Evidence suggests that similar to military combatants, military healthcare provider exposure to life-threatening situations will increase the probability of adverse psychological disorders following these traumatic experiences. The presence of a strong sense of meaning and purpose, within a supportive environment appear to help mediate the impact of these dangerous and stressful events. CONCLUSION Results of this review and other supporting literature indicate the need for a systematic approach to studying combat stress and post-traumatic stress in deployed healthcare providers.
Ileitis modulates potassium and sodium currents in guinea pig dorsal root ganglia sensory neurons

Intestinal inflammation induces hyperexcitability of dorsal root ganglia sensory neurons, which has been implicated in increased pain sensation. This study examined whether alteration of sodium (Na+) and/ or potassium (K+) currents underlies this hyperexcitability. Ileitis was induced in guinea pig ileum with trinitrobenzene sulphonic acid (TBNS) and dorsal root ganglion neurons innervating the site of inflammation were identified by Fast Blue or DiI fluorescence labelling. Whole cell recordings were made from acutely dissociated small‐sized neurons at 7–10 days. Neurons exhibited transient A‐type and sustained outward rectifier K+ currents. Compared to control, both A‐type and sustained K+ current densities were significantly reduced (42 and 34 %, respectively; P < 0.05) in labelled neurons from the inflamed intestine but not in non‐labelled neurons. A‐type current voltage dependence of inactivation was negatively shifted in labelled inflamed intestine neurons. Neurons also exhibited tetrodotoxin‐sensitive and resistant Na+ currents. Tetrodotoxin‐resistant sodium currents were increased by 37 % in labelled neurons from the inflamed intestine compared to control (P < 0.01), whereas unlabelled neurons were unaffected. The activation and inactivation curves of these currents were unchanged by inflammation. These data suggest ileitis increases excitability of intestinal sensory neurons by modulating multiple ionic channels. The lack of effect in non‐labelled neurons suggests signalling originated at the nerve terminal rather than through circulating mediators and, given that Na+ currents are enhanced whereas K+ currents are suppressed, one or more signalling pathways may be involved.
Acetabular labral limbus as a cause of hip pain: a report of five cases.

The treatment of hip disease in the young adult is a rapidly evolving and growing area of orthopaedic surgery1, driven by improved imaging techniques2,3, safe and reproducible open hip surgery4, and less invasive techniques such as hip arthroscopy5. Currently, the most common indication for hip arthroscopy is the treatment of labral abnormalities6, usually from one of four causes7: trauma, a degenerative condition, dysplasia, and impingement. Labral abnormalities are often associated with acetabular chondral damage8,9, varying in severity from a softening and/or fibrillation to complete cartilage detachment from its osseous bed. Because the treatment of the labral abnormality varies, depending on the underlying etiology and associated pathological condition, it is critical that variant anatomy of the labrum and the labral chondral junction be recognized and treated accordingly7,10. Descriptions of variations of the labral anatomy have focused mainly on the manner in which arthroscopic findings correlate with magnetic resonance imaging (MRI) findings. The primary variation has been termed sublabral sulcus, defined as a cleft between the labrum and adjacent articular cartilage. The reported location of this sublabral sulcus is variable, as is the prevalence, although it is most commonly noted anteriorly. Byrd was the first, as far as we know, to describe as “normal anomalous variations” these partial separations of the labrum from the lateral aspect of the osseous rim of the acetabulum11. However, previous studies have some limitations since the magnetic resonance arthrography (MRA) was reviewed retrospectively to determine the prevalence and location of these sulci, making the exact location difficult to interpret. We report on a clinical series of five female patients seen with mechanical hip pain secondary to an acetabular labral limbus. The clinical presentation, …
Estimating The Benefits of Mammography Screening: The Impact of Study Design

Background: Mammography screening is justifiable only if it leads to reduction in breast cancer mortality. However, evaluation of routine screening is not straightforward, as no unscreened control group is available. We report here on a cohort study of the effect of routine mammography on breast cancer mortality, and illustrate how variations in the analytic approach can affect the conclusions. Methods: We used data from the mammography screening program in Copenhagen, Denmark, for the period 1991–2001. We used local historical, concurrent regional, and historical regional control groups, and included only deaths from breast cancers diagnosed during the observation periods. We examined the impact of various control groups, of including all breast cancer deaths, and of using individual data versus routine statistics. Results: Combining all 3 control groups gave an estimated 25% reduction in breast cancer mortality. The estimate was 20% using only a local historical control group, and 9% using only a concurrent regional control group. Including all breast cancer deaths resulted in an estimate of 21% reduction in breast cancer mortality. Using routine statistics and a concurrent regional control group resulted in an estimated increase of 6% in breast cancer mortality. Conclusion: Estimated changes in breast cancer mortality following the introduction of routine mammography ranged from a 25% reduction (based on the best methodology) to a 6% increase with a less rigid study design. The estimated effect of routine mammography on breast cancer mortality is thus highly dependent on study design.
Student employment: social differentials and field-specific developments in higher education

Abstract In this article, we examine social origin differences in employment patterns across different stages of higher education and compare these differences between vocational and academic fields of study. Using data from a large-scale German student survey, we study the development of inequality, according to social origins, in student employment from first-year to graduating students. We show that inequality in job quality exists and is partly attributable to the need for students from lower social origins to work in order to finance their studies. We hypothesise that initial inequalities decrease as students progress through higher education. While we find evidence for this hypothesis, we also show in multivariate models that the reduction of inequality in the student labour market is explained by prior differences between social origin groups.
Mild Traumatic Brain Injury and Post-concussion Syndrome: Treatment and Related Sequela for Persistent Symptomatic Disease

Sport-related concussion typically resolves within a few weeks of the injury; however, persistent symptoms have been reported to occur in 10% to 15% of concussions. These ongoing symptoms can cause significant disability and be frustrating for the patient and family. In addition, factors other than brain injury can cause complications for these patients, such as adjustment disorder or exacerbation of preexisting conditions such as depression or migraine. Individuals with prolonged symptoms of concussion may be classified as having post-concussion syndrome. A careful and thoughtful evaluation is important, as the clinician must determine whether these prolonged symptoms reflect brain injury pathophysiology versus another process. Although there have been numerous studies on the acute management of concussion, much less is available on the treatment of persistent disease. This review will provide an evaluation approach for the patient with prolonged concussion symptoms and review recent literature on treatment strategies.
Reducing muscle fatigue in FES applications by stimulating with N-let pulse trains

Applications of electrical stimulation for restoration of functional movements such as standing, gait, and grasp have always been hindered by the rapid fatigue of stimulated muscle. This paper describes an experimental investigation of stimulation with N-lets (a set of N closely spaced stimulation pulses) as a means of producing contractions with improved fatigue characteristics. Experiments were conducted on 27 able-bodied and four SCI human subjects using surface stimulation of the quadriceps muscle to produce isometric knee joint torque. Based upon evidence from the literature on muscle fatigue, parameters of the N-let trains for N=1-6 were optimized to produce the most force per pulse. The results demonstrated that: 1) nonlinear summation of the twitch response occurs in human subjects with N-let surface stimulation; 2) for most subjects, doublet stimulation (N=2) with a pulse interval of about 5 ms produced the maximum torque-time integral per pulse of the resulting twitch; and 3) on average, optimal N-let stimulation resulted in a 36% increase in isometric torque tracking when compared to traditional singlet stimulation. The results have immediate implications for alleviating the problem of premature fatigue during functional electrical stimulation. <<ETX>>
Methods in Comparative Phylogeography, and Their Application to Studying Evolution in the North American Aridlands1

Abstract Historical biogeography and comparative phylogeography have much in common. Both seek to discover common historical patterns in the elements of biotas, although typically at different tiers of evolutionary history. Comparative phylogeography is based on phylogeographic analyses of multiple taxa, usually widespread species. By comparing the phylogeographic structures of numerous widespread sympatric species, one can infer whether the current fauna has been historically stable, as evidenced by the relative frequency of geographically congruent reciprocally monophyletic groups. Alternatively, if species distributions are ephemeral over evolutionary time, a mixture of phylogeographic structures is expected. Coalescence analyses contribute information about history irrespective of whether haplotype phylogenies are structured or not. In the aridlands of North America, several isolating events are evident in the phylogeographic patterns of birds, mammals and herps. A mid-peninsular seaway in Baja California, dated at ca. one million years before present, had a pervasive effect, with 13 of 16 assayed species showing a concordant split. Hence, this community appears to have been a stable assemblage of species over the past one million years. In contrast, the avifauna of the Sonoran-Chihuahuan deserts consists of two species with a concordant split and three other species that are undifferentiated across both deserts. Hence, the species in this area have had different histories. The Baja biota appears to resemble its ancestral configuration to a greater degree than the Sonoran-Chihuahuan one. A deeper evolutionary event separated taxa in Baja California from the eastern deserts, showing that the aridlands fauna was affected by events at different times resulting in overlain tiers of history.