Miracle-dz/UniFAR-FTUs huggingface dataset:
Comparison of Apo- and Heme-bound Crystal Structures of a Truncated Human Heme Oxygenase-2*

Heme oxygenase (HO) catalyzes the first step in the heme degradation pathway. The crystal structures of apo- and heme-bound truncated human HO-2 reveal a primarily α-helical architecture similar to that of human HO-1 and other known HOs. Proper orientation of heme in HO-2 is required for the regioselective oxidation of the α-mesocarbon. This is accomplished by interactions within the heme binding pocket, which is made up of two helices. The iron coordinating residue, His45, resides on the proximal helix. The distal helix contains highly conserved glycine residues that allow the helix to flex and interact with the bound heme. Tyr154, Lys199, and Arg203 orient the heme through direct interactions with the heme propionates. The rearrangements of side chains in heme-bound HO-2 compared with apoHO-2 further elucidate HO-2 heme interactions.
Adolescent Non-Suicidal Self-Injury: Analysis of the Youth Risk Behavior Survey Trends.

Kelly Emelianchik-Key, NCC, is an Assistant Professor at Florida Atlantic University. Rebekah J. Byrd, NCC, is an Assistant Professor at East Tennessee State University. Amanda C. La Guardia, NCC, is an Assistant Professor at Sam Houston State University. Correspondence can be addressed to Kelly Emelianchik-Key, Department of Counselor Education, 777 Glades Road, Building 47, Room 458, Boca Raton, FL 33431, kemelian@fau.edu. Kelly Emelianchik-Key, Rebekah J. Byrd, Amanda C. La Guardia Adolescent Non-Suicidal Self-Injury: Analysis of the Youth Risk Behavior Survey Trends
3D collaborative interaction for aerospace industry

3D collaborative interaction raises issues like awareness of the Virtual Environment that have been well known for some time. They have been the subject of research activities that have led to some interesting results described in the literature. Unfortunately, most of these results have not yet been not implemented in the software systems daily used by industry and remains only theoretical concepts. That is why we recently started a project to transfer some of these results in the aerospace industry for the Airbus Group. Beyond this first transfer target, we also intend to measure the real gains (in real industrial conditions) for the users, and then for the company. This second goal is essential because in most publications, user testing is not satisfying (lack of real users, lack of real procedures, non significant number of subjects). In this paper, we describe the first step of this work in progress and more precisely, the basic interaction features we have developed. Currently, we are designing the first user tests.
Opportunistic (L)earning in the Mobile Knowledge Society

This paper discusses the concept of opportunistic collaboration within the emerging mobile knowledge society. The paper illustrates how opportunistic collaboration can be applied to the areas of learning and earning by demonstrating how to transform a traditional unemployment agency into an Opportunistic Collaboration Agency based on an entrepreneurial supply-support network structured in the form of a prosumer manifold. The OCA pattern provides ways to capture the dynamics of entrepreneurial work-relations in the emerging 'work-portfolio society', increase the transparency of the entire value chain of economic activities, and create prosumer value loops that can support multi-dimensional bartering and increase the opportunities for marginalized groups of people to create value together. The paper ends by demonstrating how to use the OCA pattern to transform a traditional educational institution into an Opportunistic Learning Activity Broker that could help to bridge the gap between formal and informal learning.
Objective sleep in pediatric anxiety disorders and major depressive disorder.

OBJECTIVE To examine objective and subjective sleep problems in early-onset anxiety and depression. METHOD Children and adolescents (46% female, ages 7 to 17 years) with anxiety disorders (n = 24), major depressive disorder (MDD) without comorbid anxiety disorders (n = 128), or no history of psychiatric disorder (n = 101) spent two consecutive nights in a sleep laboratory and completed self-reports of sleep quality. RESULTS On objective measures, the anxiety group exhibited more awakenings than the MDD group, less slow-wave sleep than the control or MDD group, and greater night 2 sleep latency than the MDD or control group. The anxiety group exhibited no decrease in rapid eye movement latency from the first night to the second. The MDD group exhibited less time awake than the control group and less stage 1 sleep than the anxiety or control group. On subjective measures, young people with anxiety reported greater sleep latency on the second night and no decrease in sleep latency. Age was covaried in analyses. CONCLUSIONS Findings provide objective and subjective evidence of sleep disturbance in children and adolescents with anxiety disorders and replicate findings of limited objective sleep disturbance in those with MDD. Sleep problems are an important consideration when treating young people with anxiety.
A Comparative Study between Dexmedetomidine and Propofol for Fibre-optic Intubation

Background: Providing adequate anxiolysis and sedation with a patent airway while performing fiberoptic bronchoscopic intubation is a challenging task to an anaesthetist. Ideal sedation would ensure calm and cooperative patient maintaining spontaneous ventilation. Dexmedetomidine is such a α2a adrenergic agonist with sympatholytic, analgesic, and sedative properties. Though its role is very well documented for sedation, proving it better than propofol for the procedure is being considered in this study. Methods: In total of 60 patients, after nebulizing with 5 ml of 4% lignocaine over 10 minutes, 30 patients were infused with dexmedetomidine @1μg/kg over 10 minutes followed by 0. 3μg/kg and rest with propofol @1mg/kg. Fiber-optic bronchoscopy was done after 10 minutes of infusion. Monitoring was done considering heart rate, blood pressure, Ramsay sedation score and patient tolerance. Results: had shown successful intubation in both cases but dexmedetomidine had a better outcome with respect to sympathetic response and patient tolerance. P value was significant for sedation score, pre and post bronchoscopic intubation sympathetic response. No episodes of airway obstruction and hypoxia were noted with dexmedetomidine as compared with propofol. Mean Ramsay sedation score was 3.77 as compared to 2.33 with propofol. Conclusion: In our comparative study, Dexmedetomidine had offered better patient tolerance with adequate sedation and preservation of airway as compared to propofol and a reduced hemodynamic response to intubation.
Ab Initio Study on Nitrogen or Carbon Doped Magnesium Oxide

The ferromagnetism induced by nitrogen (N) or carbon (C) atoms in magnesium oxide (MgO) is studied by using ab initio calculation based on the density functional theory. The calculation shows that single N or C impurity may induce local magnetic moment to MgO due to the localization of p-electrons. Moreover, the calculation of two impurities in one supercell shows that the stability of ferromagnetism in both N:MgO and C:MgO depends on the distance between the two impurities. The energy difference between the ferromagnetic and antiferromagnetic states in some situations is smaller than 28 meV, the thermal energy at room temperature, suggesting that both the localization of the p-electrons and the distance between the impurities may influence the stability of ferromagnetism.
Salt tolerance classification of crops according to soil salinity and to water stress day index

The observations of a long-term experiment on the use of saline water were used to compare the crop tolerance to salinity. Salinity affected significantly yield, evapotranspiration, pre-dawn leaf water potential and stomatal conductance. The higher the salinity, the lower the yield, evapotranspiration, pre-dawn leaf water potential and stomatal resistance. The crop classification, based on soil salinity, corresponds with the classification of Maas and Hoffman: sugarbeet and durum wheat as salt tolerant, broadbean, maize, potato, sunflower and tomato as moderately salt sensitive. The difference with respect to soybean, classified as moderately salt sensitive instead of moderately salt tolerant can be ascribed to difference in variety. Weather conditions affected strongly the salt tolerance of broadbean. The water stress day index was also used for salt tolerance classification. According to this method, maize, sunflower and potato were included in the same salt tolerant group as sugarbeet and durum wheat. The previous classification of maize and sunflower as moderately sensitive is caused by the fact that these crops are grown during a period of higher evaporative demand than when sugarbeet and durum wheat are grown. The change of potato from moderately sensitive to salt tolerant may be ascribed to its shallow root system. # 2000 Elsevier Science B.V. All rights reserved.
Ictal and Interictal Phonophobia in Migraine—A Quantitative Controlled Study

The aim of the study was to evaluate quantitatively ictal and interictal phonophobia in episodic migraine (EM). We included subjects with EM and age- and gender-matched controls. Sound stimuli were pure tones at frequencies of 1000, 4000 and 8000 Hz. Sound aversion thresholds (SATs) were determined as the minimal sound intensity perceived as unpleasant or painful. Migraineurs were examined both between and during attacks. We compared interictal SATs in migraineurs with those in controls. We also compared ictal and interictal SATs in migraineurs. Sixty migraineurs and 52 controls were included. Interictal mean SAT of migraineurs, averaged for the three frequencies, was significantly lower than that of controls [90.4 (0.8) dB vs. 105.9 (1.1) dB, respectively, P < 0.0001]. In migraineurs, mean ictal SAT, averaged for the three frequencies, was significantly lower than interictal SAT [76.0 (0.9) dB vs. 91.0 (0.8) dB, respectively, P < 0.0001]. Patients with EM exhibit increased sound aversion between attacks that is further augmented during an acute attack.
Brownian dynamics simulations of flexible polymers with spring-spring repulsions

We develop a method which incorporates spring–spring repulsions into Brownian dynamics simulations of flexible polymers. The distance of closest approach between two springs is computed, and a repulsive force is then applied based on this distance. Repulsive potentials of the exponential and power-law forms are considered. We demonstrate that our method is capable of accounting for excluded-volume effects in start-up of extensional flow. Equilibrium simulations indicate that spring-spring repulsions can be used to prevent the passage of two springs through each other, and thus maintain the topological integrity of polymer molecules. The method developed here is expected to be useful for simulating entanglement phenomena in both single and multichain systems.
Effect of polymer adsorption on cellulose nanofibril water binding capacity and aggregation

Polymer adsorption on cellulose nanofibrils and the effect on nanofibril water binding capacity were studied using cellulose nanofibril films together with quartz crystal microbalance with dissipation (QCM-D) and surface plasmon resonance (SPR). The experiments were performed in the immersed state, and special attention was paid to the effect of polymer properties on the water content and viscoelastic properties of the polymer/fibril layer. The dry mass of the adsorbed polymers was determined using SPR. The type of the adsorbed polymer strongly affected the water content and viscoelastic properties of the nanofibril film. The adsorption of a highly charged flocculating polymer, PDADMAC, caused dehydration of the film, which was also detected as nanofibril film stiffening. The adsorption of xyloglucan introduced a dispersing effect to the nanofibril film, which was detected as a loosening and softening of the nanofibril/polymer layer. A dispersing effect was also achieved with carboxymethyl cellulose (CMC), but CMC did not adsorb irreversibly on the nanofibril surfaces. In addition to the nanofibril film studies, the effect of polymer adsorption on cellulose nanofibril suspension aggregation was demonstrated using confocal laser scanning microscopy (CLSM). Xyloglucan was shown to open the nanofibril aggregate structures and act as a dispersing agent, whereas the other polymers studied did not have as significant an effect on aggregation.
Satisfaction with formal support and education for children with autism spectrum disorder: the voices of the parents.

INTRODUCTION The aim of the present study was to identify and describe factors associated with variations in the level of parental satisfaction with formal support and education for children with autism spectrum disorder (ASD) in Flanders. METHODS Participants were recruited by a mailing from a diagnostic centre for pre-schoolers and by advertisements dispersed in the Flemish parent organization for ASD and in services and special schools. The sample consisted of 244 parents of children with ASD (age range: 2.69-17.81 years, male/female sex ratio 4:1). The data were collected using a questionnaire on experiences with education and support, which was compiled for the purpose of this study. The data resulting from the questionnaire were supplemented with information obtained from semi-structured in-depth interviews with a stratified sample of 15 parents. RESULTS AND CONCLUSIONS The study revealed that parents experienced difficulties with the diagnostic process, with support and education provided by mainstream settings and with the accessibility of autism-specific service provisions. Conversely, parents reported to be satisfied with the quality of autism-specific support and education. Predictors of overall satisfaction were parental involvement in formal support, knowledge of available service provisions and time between first consultation and final diagnosis. Practical implications of the results are discussed.
A Comparative Study on the Impacts of Interface Traps on Tunneling FET and MOSFET

In this paper, the impacts of interface traps on tunneling FET (TFET) are examined in terms of different trap energies and distributions, charge neutrality level (CNL), and effects of random trap fluctuation, in comparison with MOSFET. It is found that the Vth shifts and subthreshold swing (SS) degradation induced by interface traps in TFET and MOSFET have the same trends, but the impacts on ION are different because of the novel conduction mechanism of TFETs when compared with MOSFETs. Moreover, nTFET is intrinsically more immune (or susceptible) to Vth shift induced by acceptor(or donor-) type interface traps than nMOSFET. Therefore, reducing the potential degradation induced by the interface traps can be achieved by optimizing the position of CNL. The results indicate that nTFET is more immune to the Vth shift than nMOSFET with CNL below a critical energy. In addition, the trap-induced SS degradation of TFET is severer than MOSFET in electrostatics. Moreover, it is found that the ION, Vth, and IOFF fluctuations in nMOSFET and nTFET are also dependent on the position of CNL. With CNL below the critical energy, the ION fluctuation and Vth fluctuation of nTFET are smaller than those of nMOSFET. The results are helpful for the interface optimization of TFETs.
Organisational downsizing, sickness absence, and mortality: 10-town prospective cohort study

Abstract Objective To examine whether downsizing, the reduction of personnel in organisations, is a predictor of increased sickness absence and mortality among employees. Design Prospective cohort study over 7.5 years of employees grouped into categories on the basis of reductions of personnel in their occupation and workplace: no downsizing (< 8% reduction), minor downsizing (8-18%), and major downsizing (> 18%). Setting Four towns in Finland. Participants 5909 male and 16 521 female municipal employees, aged 19-62 years, who kept their jobs. Main outcome measures Annual sickness absence rate based on employers' records before and after downsizing by employment contract; all cause and cause specific mortality obtained from the national mortality register. Results Major downsizing was associated with an increase in sickness absence (P for trend < 0.001) in permanent employees but not in temporary employees. The extent of downsizing was also associated with cardiovascular deaths (P for trend < 0.01) but not with deaths from other causes. Cardiovascular mortality was 2.0 (95% confidence interval 1.0 to 3.9) times higher after major downsizing than after no downsizing. Splitting the follow up period into two halves showed a 5.1 (1.4 to 19.3) times increase in cardiovascular mortality for major downsizing during the first four years after downsizing. The corresponding hazard ratio was 1.4 (0.6 to 3.1) during the second half of follow up. Conclusion Organisational downsizing may increase sickness absence and the risk of death from cardiovascular disease in employees who keep their jobs.
Serum neuron-specific enolase and lactate dehydrogenase as predictors of response to chemotherapy and survival in non-small cell lung cancer.

The prognostic value of serum neuron-specific enolase (NSE) and lactate dehydrogenase (LDH) was prospectively assessed in 42 patients with locally advanced or metastatic non-small cell lung cancer (NSCLC) treated within two chemotherapy trials. Pretreatment NSE levels ranged from 4.6 to 34.6 ng/mL (median value, 7.5) and were above 10 ng/mL in ten patients (23.8%). LDH levels varied between 85 U/L and 2,484 U/L (median value, 220) and were above 250 U/L in 12 patients (29.3%). Elevated levels of both enzymes were significantly more common in patients with metastatic disease than in those with locoregional disease (40% v 9% for NSE and 40% v 18% for LDH, respectively). Strong positive correlation (correlation factor 0.693) was found between NSE and LDH serum levels. The levels of both markers did not correlate with age, sex, previous therapy, performance status, or histology. Responses to chemotherapy were seen more frequently in patients with elevated NSE levels (six of ten, 60%) than in those with normal values (seven of 32, 22%; P = .02). Similar correlation was found for LDH: Response was seen in seven of 12 patients with elevated levels (58%) and in six of 29 (21%) of those with normal values (P = .02). In a logistic regression analysis, both markers considered individually remained significant when adjusted by sex, age, performance status, prior therapy, histology, and extent of disease. Three pretreatment characteristics, high levels of NSE and LDH (both considered as continuous variables) and metastatic disease, were found to be associated with shorter survival; with adjustment for extent of disease, however, NSE and LDH were no longer correlated with shorter survival. These data suggest potential clinical value of NSE and LDH determination in treatment selection of NSCLC patients.
Human bone marrow mast cells in systemic mast cell disease

In a case of systemic mast cell disease with moderate bone marrow involvement, we studied the sensitivity of mast cell cationic dye‐binding to formaldehyde fixation, as well as the mast cell ultrastructure, so as to determine whether these cells possess characteristics of mucosal mast cells. This histochemical method has proved to be one of the few which reveal heterogeneity of the mast cells in human intestine. Even though rat bone marrow mast cells have been shown to belong to the mucosal mast cell compartment, we have found no difference in mast cell counts in samples fixed either in formaldehyde or Carnoy's fixative. The ultrastructure did not show major differences with cutaneous mast cells. A few cells presented two nuclei, suggesting mitotic division of mature mast cells in bone marrow.
Comparison of rat and human cytochrome P450 (CYP) sources of N-alkylprotoporphyrin IX. Formation after interaction with porphyrinogenic xenobiotics: studies with cDNA-expressed single CYP enzymes

1. The porphyrinogenicity of certain xenobiotics is due to mechanism-based inactivation of selected cytochrome P450 (CYP) enzymes, with concurrent formation of N -alkylprotoporphyrins (N-alkylPPs), which disrupt control of haem biosynthesis. An ambiguity arises when extrapolating results obtained with such porphyrinogenic xenobiotics in animals to humans owing to species' differences in CYP enzymes. The objective was to use cDNA-expressed individual rat CYP enzyme preparations in microsomes prepared from baculovirus-infected insect cells to determine which rat CYP enzymes were the source of N-alkylPPs after interaction with three porphyrinogenic xenobiotics and to compare the results with formation of N-alkylPPs in individual human CYP enzyme orthologues. 2. A sensitive fluorometric technique was employed to quantitate N-alkylPP formation after interaction of individual CYP enzymes with a porphyrinogenic xenobiotic. 3. N-alkylPP formation was found following the interaction of three porphyrinogenic xenobiotics with CYP1A2, 2B1, 2C6, 2C11 and 3A2, in amounts ranging from 0.45 to 0.07 nmol N-alkylPP nmol−1 CYP. The results obtained with rat CYP1A2, 2C6, 2C11 and 3A2 were compared with those previously obtained with the human CYP orthologues 1A2, 2C9 and 3A4. 4. Some results corresponded while others did not, reinforcing a previous recommendation that when dealing with xenobiotics whose porphyrinogenicity depends upon interaction with CYP enzymes resulting in N-alkylPP formation, animal experiments should be augmented with studies using human CYP preparations.
Thrombin Responses in Human Endothelial Cells

The recent identification of two new thrombin receptors, PAR3 and PAR4, led us to re-examine the basis for endothelial cell responses to thrombin. Human umbilical vein endothelial cells (HUVEC) are known to express PAR1 and the trypsin/tryptase receptor, PAR2. Northern blots detected both of those receptors and, to a lesser extent, PAR3, but PAR4 message was undetectable and there was no response to PAR4 agonist peptides. To determine whether PAR3 or any other receptor contributes to thrombin signaling in HUVEC, PAR1 cleavage was blocked with two selective antibodies and PAR1 activation was inhibited with the antagonist, BMS200261. The antibodies completely inhibited HUVEC responses to thrombin, but BMS200261 was only partly effective, even though separate studies established that the antagonist completely inhibits PAR1 signaling at the concentrations used. Since peptides mimicking the PAR1 tethered ligand domain can also activate PAR2, we asked whether the remaining thrombin response in the presence of the antagonist could be due in part to the intermolecular transactivation of PAR2 by cleaved PAR1. Evidence that transactivation can occur was obtained in COS-7 cells co-expressing PAR2 and a variant of PAR1 that can be cleaved, but not signal. There was a substantial response to thrombin only in cells expressing both receptors. Conversely, in HUVEC, complete blockade of the thrombin response by the PAR1 antagonist occurred only when signaling through PAR2 was also blocked. From these observations we conclude that 1) PAR1 is the predominant thrombin receptor expressed in HUVEC and cleavage of PAR1 is required for endothelial cell responses to thrombin; 2) although PAR3 may be expressed, there is still no evidence that it mediates thrombin responses; 3) PAR4 is not expressed on HUVEC; and 4) transactivation of PAR2 by cleaved PAR1 can contribute to endothelial cell responses to thrombin, particularly when signaling through PAR1 is blocked. Such transactivation may limit the effectiveness of PAR1 antagonists, which compete with the tethered ligand domain rather than preventing PAR1 cleavage.