Miracle-dz/UniFAR-FTUs huggingface dataset:
The feasibility of using a peer-guided model to enhance participation in community-based physical activity for youth with intellectual disability

We examined the feasibility of a using a peer-guided model to foster participation of youth with intellectual disability in community-based exercise. The intervention was designed to address personal barriers to exercise commonly faced by individuals with intellectual disability. Twenty adolescents with mild–moderate intellectual disability and 14 typically developing peers exercised in groups of two or three, 2 days per week for 15 weeks at YMCAs. Each dyad or triad provided reciprocal support during structured, individualized exercise sessions led by certified fitness trainers. Adherence to the program was high and youth with intellectual disability showed a significant reduction in personal barriers. They also felt they had learned new skills and made new friends. Typically developing youth were also positive about their experience as a volunteer. Our findings suggest that a peer-guided exercise model is useful for enhancing participation in community-based exercise.
What Rate of Infusion of Intravenous Nutrition Solution Is Required to Stimulate Uptake of Amino Acids by Peripheral Tissues in Depleted Patients?

We examined the effect of varying the quantities (0, 0.1, 0.2, 0.3, and 0.4 gN.kg-1.[day]-1) of nitrogen input on N balance, 3-methylhistidine (3MH) excretion, plasma amino acid concentration and the net flux of amino acids across the leg in depleted patients requiring parenteral nutrition. The calorie-to-nitrogen ratio was 140 to 1 (kcal:1 gN) and consequently the patients received varying amounts of calories (8, 14, 28, 42, and 56 kcal.kg-1.[day]-10. There was negative nitrogen balance and net loss of amino acids from the limb during fasting. An infusion of 0.2 gN.kg-1.[day]-1 of IVN reversed the net catabolic process and resulted in equilibrium of peripheral total amino acid flux and of tyrosine flux without a decrease in 3MH excretion. Net uptake of total amino acids and tyrosine in peripheral tissues was achieved with 0.4 gN.kg-1.[day]-1 and 56 kcal.kg-1.[day]-1. This was associated with a fivefold increase in 3MH excretion (p less than 0.01), indicating that net anabolism occurred with increased protein turnover. Fifty per cent of the amino acids taken up by peripheral tissues during infusions of 0.4 gN.kg-1. [day]-1 was due to the uptake of glutamate (Glu) and 20% was due to the uptake of branched chain amino acids (BCAA). Plasma Glu concentration, [Glu], did not increase with increasing IVN infusion, but BCAA concentrations did. Although the mean plasma [Glu] did not change with IVN infusion, there was an independent effect of plasma [Glu] (p less than 0.0001) and of N input (p less than 0.0001) on Glu flux, indicating that even at high infusion rates the maximal capacity of peripheral tissues to take up Glu had not been reached.
A receptor‐binding protein of Campylobacter jejuni bacteriophage NCTC 12673 recognizes flagellin glycosylated with acetamidino‐modified pseudaminic acid

Bacteriophage receptor‐binding proteins (RBPs) confer host specificity. We previously identified a putative RBP (Gp047) from the campylobacter lytic phage NCTC 12673 and demonstrated that Gp047 has a broader host range than its parent phage. While NCTC 12673 recognizes the capsular polysaccharide (CPS) of a limited number of Campylobacter jejuni isolates, Gp047 binds to a majority of C. jejuni and related Campylobacter coli strains. In this study, we demonstrate that Gp047 also binds to acapsular mutants, suggesting that unlike the parent phage, CPS is not the receptor for Gp047. Affinity chromatography and far‐western analyses of C. jejuni lysates using Gp047 followed by mass spectrometry indicated that Gp047 binds to the major flagellin protein, FlaA. Because C. jejuni flagellin is extensively glycosylated, we investigated this binding specificity further and demonstrate that Gp047 only recognizes flagellin decorated with acetamidino‐modified pseudaminic acid. This binding activity is localized to the C‐terminal quarter of the protein and both wild‐type and coccoid forms of C. jejuni are recognized. In addition, Gp047 treatment agglutinates vegetative cells and reduces their motility. Because Gp047 is highly conserved among all campylobacter phages sequenced to date, it is likely that this protein plays an important role in the phage life cycle.
Magnifying Success: Using the Lens of Positive Psychology/ Positive Organizational Scholarship to Examine Fundraising at Princeton Healthcare System Foundation During an Economic Recession

In this thesis, I explore the fundraising success of Princeton HealthCare System (PHCS) Foundation during the worst economic conditions in the past forty years. The lens of Positive Psychology/Positive Organizational Scholarship is uniquely useful to better understand what worked. I provide a background on PP/POS and Appreciative Inquiry and briefly discuss their history, major tenants and areas of current research. Chapters three and four further examine the application of positivity by the organization’s leadership, staff and/or volunteers and how it led to extraordinary results. A few examples of situations where Appreciative Inquiry was helpful in building consensus and shared enthusiasm are discussed. I review the implication of certain virtues such as transcendence and discuss the positive impact of its related character strengths on our stewardship program. The final chapter addresses some areas of organization weakness and related solutions garnered from my work at The Program in Organizational Dynamics. I also review and answer my thesis questions regarding the applicability of my courses to fundraising and take the liberty, in closing, to include a section of personal reflection. Comments Submitted to the Program of Organizational Dynamics in the Graduate Division of the School of Arts and Sciences in Partial Fulfillment of the Requirements for the Degree of Master of Science in Organizational Dynamics at the University of Pennsylvania Advisor: Ruth L. Orenstein This thesis or dissertation is available at ScholarlyCommons: https://repository.upenn. edu/od_theses_msod/88 MAGNIFYING SUCCESS: USING THE LENS OF POSITIVE PSYCHOLOGY/ POSITIVE ORGANIZATIONAL SCHOLARSHIP TO EXAMINE FUNDRAISING AT PRINCETON HEALTHCARE SYSTEM FOUNDATION DURING AN ECONOMIC RECESSION
Soluble vascular endothelial-cadherin and auto-antibodies to human vascular endothelial-cadherin in human diseases: Two new biomarkers of endothelial dysfunction

Vascular endothelial-cadherin is the most important transmembrane component of endothelial adherens junctions, exclusively expressed by endothelial cells in all types of vessels. Targeting either the extracellular domain or the cytoplasmic tail deleteriously affects the junctional strength and leads to vascular permeability. Recently, cytokine-induced phosphorylation of the vascular endothelial-cadherin cytoplasmic domain was reported to trigger cleavage of its extracellular domain, producing the soluble form of the protein – soluble vascular endothelial-cadherin. Hence, the presence of soluble vascular endothelial-cadherin or auto-antibodies to human vascular endothelial-cadherin in human serum could signalize the presence of vascular abnormalities. This systematic review covers many human studies reporting increased levels of soluble vascular endothelial-cadherin, as well as auto-antibodies to human vascular endothelial-cadherin, which could be promising biomarkers of endothelial dysfunction in a large panel of diseases.
Change-based inference for invariant discrimination

Under a conventional view of information processing in recurrently connected populations of neurons, computations consist in mapping inputs onto terminal attractor states of the dynamical interactions. However, there is evidence that substantial information representation and processing can occur over the course of the initial evolution of the dynamical states of such populations, a possibility that has attractive computational properties. Here, we suggest a model that explores one such property, namely, the invariance to an irrelevant feature dimension that arises from monitoring not the state of the population, but rather (a statistic of) the change in this state over time. We illustrate our proposal in the context of the bisection task, a paradigmatic example of perceptual learning for which an attractor-state recurrent model has previously been suggested. We show a change-based inference scheme that achieves near optimal performance in the task (with invariance to translation), is robust to high levels of dynamical noise and variations of the synaptic weight matrix, and indeed admits a computationally straightforward learning rule.
Dual Blockade of Renin Angiotensin System in Reducing the Early Changes of Diabetic Retinopathy and Nephropathy in a Diabetic Rat Model

Background: Diabetes mellitus is a real pandemic of the modern world and the incidence of the disease is increasing at a tremendous rate with a number of complications involving major systems of the human body. The renin angiotensin system (RAS) is considered to be involved in most of the pathological processes that result in diabetic nephropathy and retinopathy. Aim: The study was designed to evaluate and compare effects of ramipril (angiotensin-converting enzyme inhibitor-ACEI) and telmisartan (angiotensin II receptor blocker - ARBs) combinations on the progression of retinopathy and nephropathy in the streptozotocin (STZ) induced diabetic model. Materials and Methods: Diabetic state in rats was induced by chemical method using STZ 55 mg/kg intraperitoneally. Diabetic renal tubulopathy and interstitial inflammatory changes were done. Diabetic retinopathy manifested in the form of vacuolar changes in the inner plexiform and the ganglionic layers of the retina was observed. Results: Treatments with ACEI and ARBs reduced the incidence of the occurrence of cataract. The effect of combinational drugs of ACEI (ramipril) and AT1 receptor blocker (Telmisartan) was evaluated. The drugs used in combinations showed improvement in the histopathological and biochemical changes of the diabetic animals, both for the retina and kidney. Conclusion: The efficacy of the drugs suggests a pivotal role of the local RAS system in the pathogenesis of tubulopathy in the kidney and neuronal damage in the retina of the diabetic animals.
Secular trends in blood pressure during early-to-middle adulthood: the Fels Longitudinal Study

Background Some studies have shown a decline in blood pressure (BP) over the second half of the twentieth century. However, the increasing prevalence of obesity may have opposite effects on recent cohorts. Method Using serial BP data from the Fels Longitudinal Study, we examined secular trends in mean BP, the rate of change in BP with age (slopes), and the influence of obesity (i.e., BMI) and height on these trends during young-to-middle adulthood. The study sample consisted of 970 adults, aged 18–40 years, who were born between 1920 and 1979. Participants were grouped into birth decade cohorts and had up to 11 serial measurements of SBP, DBP, and BMI. Sex-stratified mixed longitudinal analyses were used to identify cohort effects on mean BP at ages 19, 29, and 39 years, and on the rate of change in BP with age. Results For both sexes, mean SBP did not vary significantly by birth cohort, before and after adjusting for height and BMI. Mean DBP exhibited a U-shaped secular trend even after adjusting for BMI and height that was influenced by age-by-cohort effects. By age 39 years, those born most recently had the highest mean DBP. Conclusion There were cohort effects on the rate of change in DBP with age, but not on rate of SBP change. The most recent cohorts had higher rates of DBP change with age compared to the earlier cohorts. The secular trend was partially influenced by the trends in BMI.
Rogowski current sensor design and analysis based on printed circuit boards (PCB)

This work presents a new Rogowski current sensor design implemented in printed circuit boards. The proposed structure utilizes a modular design for a variety of applications. Feasibility was first demonstrated using standard printed circuit boards. Test results detail performance with high repeatability with less than 0.8% difference in sensed voltages and less than 0.3% difference in sensed voltages with respect to position of installation. In order to have a less complex, more manufacturable and modular Rogowski current sensor, a flexible printed circuit (FPC) solution was also designed and demonstrated. By using the FPC solution, high sensitivity and much higher power density was achieved. Coupling analysis of a 3-phase system was also conducted both from simulation and experiments.
Improving energy efficiency in building system using a novel people localization system

This paper presents an innovative Bluetooth Low Energy indoor localization system useful to improve energy efficiency in buildings. A tracking system to localize people within a building has been developed using a novel infrastructure system and a custom signal processing method. Knowledge whether people are present or not in every room enables the automatic switching-off of lighting and heating/cooling in empty room increasing the energy efficiency of building system. The implemented method achieves performance comparable to that of a traditional Bluetooth Low Energy indoor location system, nevertheless fewer devices are used. As a result, the cost of installation in existing building decreases.
Unraveling the Nexus: Exploring the Pathways to Combined Resource Use

In response to the unprecedented decline in global natural resource endowments, the so‐called nexus framework is gaining increasing influence on resource management practices. In this research, we approach the resource nexus through the concept of nexus pathways. Nexus pathways are configurations that resource flows follow along supply chains leading to the combined use of two or more resources. Three general types of pathways are identified: direct (on‐site use), dependent (one‐way supply chains), and interdependent (supply‐chain feedbacks). We quantify and compare each pathway by means of multiregional input‐output analysis and structural path analysis, and apply this approach to a comparative case study on the water‐energy nexus (WEN) in the United States and China. Interdependencies or feedbacks are generally thought to be relevant for the WEN, especially between water and energy sectors. Our economy‐wide analysis for both countries indicates, however, that feedbacks neither play an important role in the WEN nor substantially take place between water and energy sectors. The most important feedbacks contribute to less than 1% of total resource use, and these take place mostly between manufacturing sectors. Overall, the studied WEN is mostly driven by dependent pathways and, to a lesser degree, direct resource use. Comparative differences between the two countries are largely explained by differences in economic structure, technology, and resource endowments. Our findings call into question current research and policy focus and suggest greater attention to less complex, but more determining, pathways leading to absolute resource use.
The Korea Employersâ€™ Federation and the development of Korean industrial relations

This chapter explains how and why chaebols founded Koreaâ€™s first national employer association, the Korea Employers Federation (KEF) in 1970, despite facing little threat from unions or pro-employee government intervention. It then explores the KEFâ€™s changing roles in responding to chaebolsâ€™ expectations up to 2010. As chaebols grew and moved into new industries, their main industrial relations interests were maintaining low labour costs and unimpeded workplace control. In this, they had government support until Koreaâ€™s democratization in 1987. Needing little collective leadership, they instead sought increasing technical expertise from KEF staff. With democratization, chaebols received much less government support. Industrial relations now included independent unionism and militancy, rising wages, and legislative support for collective bargaining and individual employee rights. Chaebols now wanted KEF representation, which became significant for lobbying governments, negotiating with peak unions and within the Tripartite Commission. Nonetheless, its lack of authority over chaebols limited its strategies.
The genetics of motor neuron diseases

Motor neuron diseases may be divided into three categories: those with lower motor neuron involvement – spinal muscular atrophy (SMA) and spinobulbar muscular atrophy (SBMA or Kennedy's disease); those with upper motor neuron involvement – primary lateral sclerosis (PLS)1,2 and the spastic paraplegias; and those with combined upper and lower motor neuron involvement – amyotrophic lateral sclerosis (ALS). Other familial motor neuron disorders include hereditary neuronopathies, GM2 gangliosidosis, and possibly the ALS/PD syndrome of Guam. The contribution of genetics to the etiopathogenesis of motor neuron disorder varies considerably, accounting for a high percentage of spinal muscular atrophies, but only a small fraction of cases of ALS. The mode of inheritance also varies, with examples of autosomal dominant (AD), autosomal recessive (AR), or X‐linked kindreds. (Tables 1 and 2).
Proteins PblA and PblB of Streptococcus mitis, Which Promote Binding to Human Platelets, Are Encoded within a Lysogenic Bacteriophage

ABSTRACT The binding of platelets by bacteria is a proposed central mechanism in the pathogenesis of infective endocarditis. Platelet binding by Streptococcus mitis strain SF100 (an endocarditis isolate) was recently shown to be mediated in part by the surface proteins PblA and PblB. The genes encoding PblA and PblB are clustered with genes nearly identical to those of streptococcal phages r1t, 01205, and Dp-1, suggesting that pblA andpblB might reside within a prophage. To address this possibility, cultures of SF100 were exposed to either mitomycin C or UV light, both of which are known to induce the lytic cycle of many temperate phages. Both treatments caused a significant increase in the transcription of pblA. Treatment with mitomycin C or UV light also caused a substantial increase in the expression of PblA and PblB, as detected by Western blot analysis of proteins in the SF100 cell wall. By electron microscopy, phage particles were readily visible in the supernatants from induced cultures of SF100. The phage, designated SM1, had a double-stranded DNA genome of approximately 35 kb. Southern blot analysis of phage DNA indicated thatpblA and pblB were contained within the SM1 genome. Furthermore, Western blot analysis of phage proteins revealed that both PblA and PblB were present in the phage particles. These findings indicate that PblA and PblB are encoded by a lysogenic bacteriophage, which could facilitate the dissemination of these potential virulence determinants to other bacterial pathogens.
Purified cofactors and histone H1 mediate transcriptional regulation by CTF/NF-I

The initiation of RNA polymerase II transcription is controlled by DNA sequence-specific activator proteins, in combination with cofactor polypeptides whose function is poorly understood. Transcriptional cofactors of the CTF-1 activator were purified on the basis of their affinity for the regulatory protein. These purified cofactors were found to be required for CTF-1-regulated transcription, and they counteracted squelching by an excess of activator in in vitro reconstitution experiments. Interestingly, the cofactors possessed an inhibitory activity for basal transcription, which was relieved by the further addition of the activator. Histone H1 also contributes to the regulation of transcription by CTF-1, whereby the activator prevents repression of the basal transcription machinery by the histone. However, histone H1 could not replace the cofactors for CTF-1-regulated transcription, indicating that they possess distinct transcriptional properties. Furthermore, the purified cofactors were found to be required, together with the activator, in order to antagonize the histone-mediated repression of transcription. These results suggest that CTF-1 and its cofactors function by regulating the assembly of the basal transcription machinery onto the promoter when the latter is in competition with DNA-binding inhibitory proteins such as histone H1.
Molecular Cloning and Physical Mapping of Restriction Endonuclease Fragments of Autographa californica Nuclear Polyhedrosis Virus DNA

A restriction fragment library containing Autographa californica nuclear polyhedrosis virus (AcNPV) DNA was constructed by using the pBR322 plasmid as a vector. The library, which is representative of more than 95% of the viral genome, consists of 2 of the 7 BamHI fragments, 12 of the 24 HindIII fragments, and 23 of the 24 EcoRI fragments. The cloned fragments were characterized and used to generate physical maps of the genome by hybridizing nick-translated recombinant plasmid to Southern blots of AcNPV DNA digested with SmaI, BamHI, XhoI, PstI, HindIII, and EcoRI restriction endonucleases. This information was used to define our strain of AcNPV (HR3) with respect to other strains for which physical maps have been previously published. The hybridization data also indicate that reiteration of DNA sequences occurs at the HindIII-L and -Q regions of the genome.
Efficacy of Newcastle Disease Virus Recombinant Expressing Avian Influenza Virus H6 Hemagglutinin Against Newcastle Disease and Low Pathogenic Avian Influenza in Chickens and Turkeys

Abstract A recombinant Newcastle disease virus (NDV) expressing H6 hemagglutinin (HA) of a low pathogenic avian influenza virus (LPAIV) was generated by reverse genetics (NDVH6). The H6 open reading frame was inserted as an additional transcription unit between the fusion and hemagglutinin-neuraminidase (HN) gene of lentogenic NDV clone 30. Expression of the foreign gene was demonstrated by northern blot, western blot, and indirect immunofluorescence analyses. The protective efficacy against Newcastle disease and avian influenza of subtype H6 was evaluated in 3-wk-old chickens and turkeys. A single vaccination protected specific-pathogen-free (SPF) chickens against a subsequent lethal NDV infection and prevented shedding of AIV after homologous H6 LPAIV infection. Furthermore, vaccinated and AIV-infected animals could be differentiated by detection of AIV nucleoprotein-specific antibodies. Three-week-old commercial turkeys, exhibiting NDV-specific maternal antibodies, were partially protected against a lethal NDV challenge infection. The mortality rate of NDVH6-immunized turkeys was reduced to 40% compared to 90% in unvaccinated birds. After H6 LPAIV infection, shedding in NDVH6-immunized turkeys was only marginally reduced compared to NDV-immunized control birds. We previously described HA-expressing NDV recombinants as potent bivalent vaccines against Newcastle disease and highly pathogenic avian influenza of subtype H5 or H7. The results presented here are in contrast to the high protective efficacy in SPF chickens, as a single vaccination with NDVH6 was insufficient in turkeys in the presence of maternal antibodies against NDV. Therefore, the vector virus has to be improved to overcome these limitations.