Miracle-dz/UniFAR-FTUs huggingface dataset:
Building Large Monolingual Dictionaries at the Leipzig Corpora Collection: From 100 to 200 Languages

The Leipzig Corpora Collection offers free online access to 136 monolingual dictionaries enriched with statistical information. In this paper we describe current advances of the project in collecting and processing text data automatically for a large number of languages. Our main interest lies in languages of low density, where only few text data exists online. The aim of this approach is to create monolingual dictionaries and statistical information for a high number of new languages and to expand the existing dictionaries, opening up new possibilities for linguistic typology and other research. Focus of this paper will be set on the infrastructure for the automatic acquisition of large amounts of monolingual text in many languages from various sources. Preliminary results of the collection of text data will be presented. The mainly language-independent framework for preprocessing, cleaning and creating the corpora and computing the necessary statistics will also be depicted.
Biomaterials for Brain Tissue Engineering

Neurological disorders such as traumatic brain injuries or stroke result in neuronal loss and disruption of the brain parenchyma. Current treatment strategies are limited in that they can only mitigate the degeneration process or alleviate the symptoms but do not reverse the condition. In contrast, regenerative cell-based therapies offer long-term hope for many patients. Bioactive scaffolds are likely to reinforce the success of cell replacement therapies by providing a microenvironment that facilitates the survival, proliferation, differentiation, and connectivity of transplanted and/or endogenous cells. This Review outlines various biomaterials (including hydrogels, self-assembling peptides, and electrospun nanofibres) that have been investigated for the repair of brain tissue, and discusses strategies for the immobilization of biomolecules. An overview of the potential clinical applications of such scaffolds in neurodegenerative diseases is also provided.
Genotypic Characterization of Coagulase-negative Staphylococci Isolated from Sheep Milk in Slovakia

Hitherto very few reports are available presenting identification and molecular characterization of the coagulase negative staphylococci (CNS) from sheep milk in the subclinical stage of mastitis. Furthermore, very scanty data are available on the epidemiological status of CNS in different Slovak provinces. Milk samples from 54 sheep farms located in eastern Slovak region were screened. A total 240 CNS were identified with series of biochemical testes (STAPH-API) and subjected further for genotyping with the help of pulse field gel electrophoresis (PFGE). The most frequently occurring CNS species according the biochemical characterization were: S. epidermidis (36.3 %), S. caprae (21.3 %), S. hominis (6.6 %), S. chromogenes (6.3 %), S. xylosus (5.8 %), S. warneri (5.0 %) and S. capitis (4.6 %). Further PFGE-based characterization of these isolates revealed six pulsotypes of the S. epidermidis, two of S. caprae, three of S. chromogenes, nine of S. hominis, five of S. capitis and seven of S. xylosus. These results contribute to knowledge of the epidemiological situation of the CNS from the subclinical form of mastitis in Slovakia. CNS, PFGE, pulsotype, mastitis, sheep
Diverse transport modes by the solute carrier 26 family of anion transporters

The solute carrier 26 (SLC26) transporters are anion transporters with diverse substrate specificity. Several members are ubiquitous while others show limited tissue distribution. They are expressed in many epithelia and to the extent known, play a central role in anion secretion and absorption. Members of the family are primarily Cl− transporters, although some members transport mainly SO42−, Cl−, HCO3− or I−. A defining feature of the family is their functional diversity. Slc26a1 and Slc26a2 function as specific SO42− transporters while Slc26a4 functions as an electroneutral Cl−/I−/HCO3− exchanger. Slc26a3 and Slc26a6 function as coupled electrogenic Cl−/HCO3− exchangers or as bona fide anion channels. SLC26A7 and SLC26A9 function exclusively as Cl− channels. This short review discusses the functional diversity of the SLC26 transporters.
Characteristic Flavonoids from Acacia burkittii and A. Acuminata Heartwoods and their Differential Cytotoxicit to Normal and Leukemia Cells

Bioassay-guided fractionation of extracts from Acacia burkittii and A. acuminata heartwoods against an L1210 (mouse lymphoma) cell line led to the isolation of two flavan-3,4-diols, melacacidin (1) and isomelacacidin (2), and three flavonoids, 3,7,8,3′,4′-pentahydroxyflavone (3), 7,8,3′,4′-tetrahydroxyflavanone (4) and 3,7,8,3′,4′-pentahydroxyflavanone (5). HPLC analyses (280 nm) of the 70% acetone extracts of the two plants showed different profiles in terms of the relative concentration of the five identified compounds. When tested against L1210, compounds 1 and 2 were the most active, giving ID50 values of 2.5 and 4.5 μg/mL, respectively. The lower activity of the other isolated compounds indicated the importance of the 3,4-hydroxyl groups for the cytotoxic activity of these flavonoids. The isolated compounds were either non-toxic or had very low toxicities against the “normal” CV-1 cell line (green monkey kidney cells). The O-methyl and O-acetyl derivatives of these compounds were inactive. Ten commercially available phenolic compounds (6-15) were also tested for their activity against both CV-1 and L1210 cell lines. Flavan-3,4-diols 1 and 2 were more cytotoxic to L1210 cells than all tested compounds, including catechin and epicatechin, the most abundant flavan-3-ols in many fruits and vegetable.
LOCAL MODELS—THE KEY TO BOOSTING STABLE LEARNERS SUCCESSFULLY

Boosting has been shown to improve the predictive performance of unstable learners such as decision trees, but not of stable learners like Support Vector Machines (SVM), k‐nearest neighbors and Naive Bayes classifiers. In addition to the model stability problem, the high time complexity of some stable learners such as SVM prohibits them from generating multiple models to form an ensemble for large data sets. This paper introduces a simple method that not only enables Boosting to improve the predictive performance of stable learners, but also significantly reduces the computational time to generate an ensemble of stable learners such as SVM for large data sets that would otherwise be infeasible. The method proposes to build local models, instead of global models; and it is the first method, to the best of our knowledge, to solve the two problems in Boosting stable learners at the same time. We implement the method by using a decision tree to define local regions and build a local model for each local region. We show that this implementation of the proposed method enables successful Boosting of three types of stable learners: SVM, k‐nearest neighbors and Naive Bayes classifiers.
Food webs and niche space.

What is the minimum dimension of a niche space necessary to represent the overlaps among observed niches? This book presents a new technique for obtaining a partial answer to this elementary question about niche space. The author bases his technique on a relation between the combinatorial structure of food webs and the mathematical theory of interval graphs. Professor Cohen collects more than thirty food webs from the ecological literature and analyzes their statistical and combinatorial properties in detail. As a result, he is able to generalize: within habitats of a certain limited physical and temporal heterogeneity, the overlaps among niches, along their trophic (feeding) dimensions, can be represented in a one-dimensional niche space far more often than would be expected by chance alone and perhaps always. This compatibility has not previously been noticed. It indicates that real food webs fall in a small subset of the mathematically possible food webs. Professor Cohen discusses other apparently new features of real food webs, including the constant ratio of the number of kinds of prey to the number of kinds of predators in food webs that describe a community. In conclusion he discusses possible extensions and limitations of his results and suggests directions for future research.
A Higher Plant Mitochondrial Homologue of the Yeast m-AAA Protease

Mitochondrial AAA metalloproteases play a fundamental role in mitochondrial biogenesis and function. They have been identified in yeast and animals but not yet in plants. This work describes the isolation and sequence analysis of the full-length cDNA from the pea (Pisum sativum) with significant homology to the yeast matrix AAA (m-AAA) protease. The product of this clone was imported into isolated pea mitochondria where it was processed to its mature form (PsFtsH). We have shown that the central region of PsFtsH containing the chaperone domain is exposed to the matrix space. Furthermore, we have demonstrated that the pea protease can complement respiration deficiency in the yta10 and/oryta12 null yeast mutants, indicating that the plant protein can compensate for the loss of at least some of the important m-AAA functions in yeast. Based on biochemical experiments using isolated pea mitochondria, we propose that PsFtsH-like m-AAA is involved in the accumulation of the subunit 9 of the ATP synthase in the mitochondrial membrane.
IMMUNOHISTOCHEMICAL STUDY ON THE PATTERN OF 50 KD CYTOKERATIN IN PSORIASIS

Three biopsies of normal skin and 15 biopsies collected from patients with psoriasis vulgaris were analyzed for the expression of the 50 kd cytokeratin using direct immunofluorescence and ABC technique before and after local treatment with anthralin 0.1% in a petroiatum base, with 0.05% betamethasone dipropionate cream, and finally, after PUVA treatment. Antiserum against the 50 kd anticytokeratin reacted with tissue sections of normal skin, staining cells in the basal layer, while the psoriatic skin sections before the various treatments showed a staining concerning the whole thickness of the epidermis. After the various therapies, the 50 kd cytokeratin immunoreactivity was observed only in the basal layer of those psoriatic skin sections that showed complete clinical clearing, while it was observed in the whole thickness of psoriatic patches that did not clear. These data suggest that the normalization of the 50 kd cytokeratin expression pattern can be considered as a marker of clinical remission of psoriasis.
Central role of eNOS in the maintenance of endothelial homeostasis.

SIGNIFICANCE Disruption of endothelial function is considered a key event in the development and progression of atherosclerosis. Endothelial nitric oxide synthase (eNOS) is a central regulator of cellular function that is important to maintain endothelial homeostasis. RECENT ADVANCES Endothelial homeostasis encompasses acute responses such as adaption of flow to tissue's demand and more sustained responses to injury such as re-endothelialization and sprouting of endothelial cells (ECs) and attraction of circulating angiogenic cells (CAC), both of which support repair of damaged endothelium. The balance and the intensity of endothelial damage and repair might be reflected by changes in circulating endothelial microparticles (EMP) and CAC. Flow-mediated vasodilation (FMD) is a generally accepted clinical read-out of NO-dependent vasodilation, whereas EMP are upcoming prognostically validated markers of endothelial injury and CAC are reflective of the regenerative capacity with both expressing a functional eNOS. These markers can be integrated in a clinical endothelial phenotype, reflecting the net result between damage from risk factors and endogenous repair capacity with NO representing a central signaling molecule. CRITICAL ISSUES Improvements of reproducibility and observer independence of FMD measurements and definitions of relevant EMP and CAC subpopulations warrant further research. FUTURE DIRECTIONS Endothelial homeostasis may be a clinical therapeutic target for cardiovascular health maintenance.
Relative expression of Slug, RhoB, and HNK‐1 in the cranial neural crest of the early chicken embryo

The neural crest constitutes a complex population of cells that originates at the edges of the neural plate of vertebrate embryos and gives rise to a high diversity of tissues and cell types. Molecular markers are very useful to identify cell populations, and in the case of the neural crest at early stages, many of them have been described. Here, we show a series of chicken embryos double labeled for several of the most commonly used crest markers that evidence the existence of different subpopulations. Slug is a very good marker for premigratory and early migratory cranial neural crest, RhoB labels delaminating cells and the very early migratory population, and the HNK‐1 epitope is acquired in the migratory crest cells at a distance from the neural tube, with a significant proportion of the Slug‐expressing migratory cells negative for HNK‐1. The existence of these crest subpopulations should be considered when analyzing both wild‐type embryos and the phenotype of experimentally manipulated chick embryos. Developmental Dynamics 229:136–139, 2004. © 2003 Wiley–Liss, Inc.
CHARACTERIZATION OF MAMMARY GLAND TISSUE USING JOINT ESTIMATORS OF MINKOWSKI FUNCTIONALS

A theoretical approach to estimate the Minkowski functionals, i.e., area fraction, specifc boundary length and specifc Euler number in 2D, and their asymptotic covariance matrix proposed by Spodarev and Schmidt (2005) and Pantle et al. (2006a;b) is applied to real image data. These two-dimensional images show mammary gland tissue and should be classifed automatically as tumor-free or mammary cancer, respectively. The estimation procedure is illustrated step-by-step and the calculations are described in detail. To reduce dependencies from chosen parameters, a least-squares approach is considered as recommended by Klenk et al. (2006). Emphasis is placed on the detailed description of the estimation procedure and the application of the theory to real image data.
Structure Prediction of Simple Non-standard Pseudoknot

The secondary structure of an RNA molecule is known to be critical in its biological function. However, the problem of predicting the secondary structure of an RNA molecule based on its primary sequence is computationally difficult in the presence of pseudoknots. In general, the problem is NP-hard. Most of the existing algorithms aim at restricted classes of pseudoknots. In this paper, we consider a new class of pseudoknot structures, called simple non-standard pseudoknot, which can cover more complicated secondary structures found in existing databases. None of the previous algorithms can handle this class of pseudoknots. Only two of them, which run inO(m6) andO(m5) time where m is the length of the given RNA sequence, can handle certain cases in this new class. On the other hand, we provide a prediction algorithm that runs in O(m4) time for simple non-standard pseudoknots of degree 4 which already covers all known secondary structures of RNAs in this class.
Path Integration in Desert Ants Controls Aggressiveness

Cataglyphis ants of the Sahara Desert are extremely aggressive toward members of other colonies if they are close to their nest entrance, but not once they have embarked on their wide foraging journeys. We show that the ants reach the same level of aggressiveness when they are far from their actual nesting site but their path integrator--their main navigational toolkit--tells them that they are close, indicating a role for the animal's path integrator beyond navigation.
Canine tracheal epithelial cells express the type 1 insulin-like growth factor receptor and proliferate in response to insulin-like growth factor I.

Disaggregated airway epithelial cells replicate in serum-free media containing supraphysiologic concentrations of insulin. To examine the hypothesis that the type 1 insulin-like growth factor (IGF) receptor mediates the mitogenic action of insulin on these cells, we studied the mitogenic effects of IGF-I and insulin, and the expression of type 1 IGF receptors in primary cultures of adult canine tracheal epithelial cells. Isolated tracheal epithelial cells were grown in varying concentrations of IGF-I or insulin in Ham's F12 medium supplemented with transferrin, cholera toxin, and endothelial cell growth supplement. Both IGF-I and insulin increased DNA synthesis (measured as [3H]thymidine incorporation into DNA) and cell number in a concentration-dependent fashion, but IGF-I was at least 20 to 60 times more potent than insulin in its mitogenic effects. No additive or synergistic effect was observed with the simultaneous addition of IGF-I and insulin in maximally effective doses. A monoclonal antibody directed against the type 1 IGF receptor (alpha IR3) blocked the mitogenic activity of both IGF-I and insulin. Affinity labeling of type 1 IGF receptors by covalent cross-linking with disuccinimidyl suberate demonstrated the tracheal epithelial cell IGF-I binding moiety to have a relative molecular weight of 130,000 D. Binding of [125I]IGF-I to this protein was inhibited by low concentrations of IGF-I, relative to insulin, and by alpha IR3. An 11-kb transcript characteristic of mRNA for the type 1 IGF receptor was recognized in poly(A+) RNA derived from cultured canine tracheal epithelial cells. (ABSTRACT TRUNCATED AT 250 WORDS)
Nucleocytoplasmic transport in the yeast Saccharomyces cerevisiae.

INTRODUCTION. 219 GENERAL FEATURES OF MOVEMENT OF PROTEINS AND RNA BETWEEN THE NUCLEUS AND CYTOPLASM. 220 Entry of Proteins into the Nucleus . 220 Export of RNA from the Nucleus . 222 COMPOSITION AND DYNAMICS OF THE YEAST NUCLEAR ENVELOPE . 222 Structure of the Yeast Nucleus . 222 Behavior of the Nuclear Envelope During Mitosis in Yeast. 227 Nuclear Pore Complexes in Yeast . 228 RECOGNITION OF PROTEINS FOR IMPORT INTO THE YEAST NUCLEUS . 232 Nuclear Localization Sequences . 232 In vitro Studies of Nuclear Transport with Yeast . 237 Yeast Proteins that Bind Nuclear Localization Sequences. 239 IN VIVO STUDIES OF NUCLEAR TRANSPORT 241 Nuclear Protein Localization Mutants. 241 RNA Export Mutants . 243 REGULATION OF NUCLEAR PROTEIN UPTAKE 245 Swi5 . 245 Ace2 . 246 Cdc46 . 246 Glucocorticoid Receptor . 247 CONCLUSIONS . 248
Mice Lacking the Integrin β5 Subunit Have Accelerated Osteoclast Maturation and Increased Activity in the Estrogen‐Deficient State

Integrin αvβ5 is expressed on osteoclast precursors and is capable of recognizing the same amino acid motif as αvβ3. Three‐month‐old β5−/− female OVX mice had increased osteoclastogenesis ex vivo, and μCT assessment of trabecular bone volume was 53% lower than WT‐OVX animals. These preliminary data suggest αvβ5 integrin's presence on osteoclast precursors may inhibit of osteoclast formation.
Platelet Rich Plasma: Biology and New Technology

Platelets play a central role in hemostasis and wound healing. The latter is mediated by release of secretory proteins on platelet activation, which directly or indirectly influences virtually all aspects of the wound healing cascade. Studies in basic science have shown a dose-response relationship between the platelet concentration and levels of secretory proteins, as well as between platelet concentration and certain proliferative events of significance to the healing wound. Technologies to provide autologous platelet rich plasma to the repair site are now being used in a wide variety of clinical applications, with the majority of such studies suggesting a role in the surgeon's armamentarium. Little standardization in the field exists, which has made it difficult to fully evaluate the literature on the subject and unequivocally establish applications for which the technology truly has merit. This article presents fundamental background on platelet biology and the role of platelets in both hemostasis and wound healing, as well as methods of preparing, characterizing, and using platelet rich plasma, to provide the reader a foundation on which to critically evaluate prior studies and plan future work.
A Meta‐Analysis of the Effects of Common Management Actions on the Nest Success of North American Birds

Abstract: Management strategies for the recovery of declining bird populations often must be made without sufficient data to predict the outcome of proposed actions or sufficient time and resources necessary to collect these data. We quantitatively reviewed studies of bird management in Canada and the United States to evaluate the relative efficacy of 4 common management interventions and to determine variables associated with their success. We compared how livestock exclusion, prescribed burning, removal of predators, and removal of cowbirds (Molothrus ater) affect bird nest success and used meta‐regression to evaluate the influence of species and study‐specific covariates on management outcomes. On average, all 4 management interventions increased nest success. When common species and threatened, endangered, or declining species (as defined by long‐term trend data from the North American Breeding Bird Survey) were analyzed together, predator removal was the most effective management option. The difference in mean nest success between treatment and control plots in predator‐removal experiments was more than twice that of either livestock exclusion or prescribed burning. However, when we considered management outcomes from only threatened, endangered, or declining species, livestock exclusions resulted in the greatest mean increase in nest success, more than twice that of the 3 other treatments. Our meta‐regression results indicated that between‐species variation accounted for approximately 86%, 40%, 35%, and 7% of the overall variation in the results of livestock‐exclusion, prescribed‐burn, predator‐removal, and cowbird‐removal studies, respectively. However, the covariates we tested explained significant variation only in outcomes among prescribed‐burn studies. The difference in nest success between burned and unburned plots displayed a significant, positive trend in association with time since fire and was significantly larger in grasslands than in woodlands. Our results highlight the importance of comparative studies on management effects in developing efficient and effective conservation strategies.
Whole genomic characterization of a Korean human parechovirus type 1 (HPeV1) identifies recombination events

A human parechovirus (HPeV) strain CAU10‐NN was detected from a stool sample of a 2‐year‐old healthy female infant in South Korea using a metagenomic approach. The CAU10‐NN virus was isolated using a cell culture system and its whole genome was analyzed. The RNA genome of the CAU10‐NN strain consists of 7,348 nucleotides (nt), excluding a poly(A) tail. A large open reading frame of 6,540 nt that encodes a putative polyprotein precursor of 2,180 amino acids is flanked by a 5′‐untranslated region (UTR) of 708 nt and 3′‐UTR of 88 nt followed by a poly(A) tail. Phylogenetic analysis shows that the CAU10‐NN strain belongs to HPeV1. SimPlot and Bootscan analyses reveal that the virus genome is composed of regions related to corresponding genomic regions of other HPeVs. Recombination analysis indicates that the CAU10‐NN strain might be a product of more than one genomic recombination event that occurred among HPeV1, HPeV3, and HPeV4 strains. J. Med. Virol. 86:2084–2091, 2014. © 2014 Wiley Periodicals, Inc.