Miracle-dz/UniFAR-FTUs huggingface dataset:
Changes in Motor Skill Proficiency After Equine-Assisted Activities and Brain-Building Tasks in Youth With Neurodevelopmental Disorders

There is a lack of current research to support the efficacy of a combination of equine-assisted activities (EAA) and brain building activities to influence motor skill competencies in youth with neurodevelopmental disorders (ND). The primary objective of this study was to quantify changes in motor skill proficiency before and after 8 weeks of EAA and brain-building activities in youth with ND. A secondary objective was to quantify changes in motor skill proficiency before and after 1 year of EAA and brain-building activities in youth with ND. Twenty-five youth completed the same 32-week protocol that was separated into 4, 8-week blocks, in the following order: (1) control; (2) EAA-only; (3) washout; (4) GaitWay block (EAA and brain building activities). Before and after each block, motor skills were assessed using the Short Form of the Bruininks-Oseretsky Test of Motor Proficiency-Version 2 (BOT-2). Seven youth continued with the GaitWay intervention for one additional year, and the BOT-2 Short Form was also administered following this intervention. A repeated-measures analysis-of-variance was performed to compare BOT-2 subtest and overall scores between interventions with a significance of 0.05. Manual dexterity was higher at Post-Washout [3.3 (2.4)] vs. Pre-Control [2.2 (2.1); p = 0.018] and Post-Control [2.6 (2.0); p = 0.024], and at Post-GaitWay vs. Pre-Control [3.2 (2.4) vs. 2.2 (2.1); p = 0.037]. Upper-limb coordination was higher at Post-GaitWay vs. Post-Control [6.0 (4.1) vs. 3.9 (3.8); p = 0.050]. When compared to Pre-Control [3.2 (3.0)], strength was higher at Post-EAA [4.9 (3.5); p = 0.028] and at Post-GaitWay [5.2 (2.9); p = 0.015]. Overall scores were higher at Post-GaitWay [39.1 (22.2)] when compared to Pre-Control [32.4 (21.6); p = 0.003] and Post-Control [32.5 (21.9); p = 0.009]. Additionally, motor skills were maintained for 1 year following the Post-GaitWay testing session among seven participants. This is the first known study to include and demonstrate the short-term and long-term effects of a combination of EAA and brain building activities with motor proficiency in youth with ND. Clinical Trial Registration: Motor Skill Proficiency After Equine-Assisted Activities and Brain-building Tasks; www.ClinicalTrials. gov, identifier: NCT04158960.
Effect of Guanxinning Tablet on Hemorrheology in Aged Rats

Objective To observe the effect of Guanxinning tablet on hemorrheology and correlative biochemical indexes in aged rats.Methods Fifty 15-month-old male SD rats were randomly divided into 5 groups including a model control group,a positive control group and low (0.3 g·kg~(-1)),middle (0.6g·kg~(-1)) and high (1.2 g·kg~(-1)) dose groups of Guanxinning tablet.An additional ten 3-month-old rats were used as the normal control group.Determine the hemorrheology and the biochemical indexes of glucose (GLU),total cholesterol (TC),triglyeride (TG),superoxide dismutase (SOD) and malondialdehyde (MDA) in blood serum of rats after 15 days.Results Guanxinning tablet with low, middle and high dose had significant depressed effect on the level of blood viscosity,plasma viscosity, erythrocyte rigidity index,and significant elevated effect on the activity of SOD and depressed effect on the content of MDA in serum of aged rats,Guanxinning tablet with high dose had significant depressed effect on the level of TC and GLU.Conclusion Guanxinning tablet had significant amendatory effect on the abnormity of blood viscosity and erythrocyte rheological characteristic in aged rats,and contributed to inhibit the peroxidation of free radical,protect the activity of anti-oxidative enzyme,boost elimination ability for free radical.In a word,Guanxirming tablet had the characteristic of promoting blood circula- tion by removing blood stasis.
Strategies for the Treatment of Parkinson’s Disease: Beyond Dopamine

Parkinson’s disease (PD) is the second-leading cause of dementia and is characterized by a progressive loss of dopaminergic neurons in the substantia nigra alongside the presence of intraneuronal α-synuclein-positive inclusions. Therapies to date have been directed to the restoration of the dopaminergic system, and the prevention of dopaminergic neuronal cell death in the midbrain. This review discusses the physiological mechanisms involved in PD as well as new and prospective therapies for the disease. The current data suggest that prevention or early treatment of PD may be the most effective therapeutic strategy. New advances in the understanding of the underlying mechanisms of PD predict the development of more personalized and integral therapies in the years to come. Thus, the development of more reliable biomarkers at asymptomatic stages of the disease, and the use of genetic profiling of patients will surely permit a more effective treatment of PD.
Genome-Wide DNA Methylation Patterns in Persistent Attention-Deficit/Hyperactivity Disorder and in Association With Impulsive and Callous Traits

Attention-deficit/hyperactivity disorder (ADHD) is a neurodevelopmental disorder that often persists into adulthood. ADHD and related personality traits, such as impulsivity and callousness, are caused by genetic and environmental factors and their interplay. Epigenetic modifications of DNA, including methylation, are thought to mediate between such factors and behavior and may behave as biomarkers for disorders. Here, we set out to study DNA methylation in persistent ADHD and related traits. We performed epigenome-wide association studies (EWASs) on peripheral whole blood from participants in the NeuroIMAGE study (age range 12–23 years). We compared participants with persistent ADHD (n = 35) with healthy controls (n = 19) and with participants with remittent ADHD (n = 19). Additionally, we performed EWASs of impulsive and callous traits derived from the Conners Parent Rating Scale and the Callous-Unemotional Inventory, respectively, across all participants. For every EWAS, the linear regression model analyzed included covariates for age, sex, smoking scores, and surrogate variables reflecting blood cell type composition and genetic background. We observed no epigenome-wide significant differences in single CpG site methylation between participants with persistent ADHD and healthy controls or participants with remittent ADHD. However, epigenome-wide analysis of differentially methylated regions provided significant findings showing that hypermethylated regions in the APOB and LPAR5 genes were associated with ADHD persistence compared to ADHD remittance (p = 1.68 * 10−24 and p = 9.06 * 10−7, respectively); both genes are involved in cholesterol signaling. Both findings appeared to be linked to genetic variation in cis. We found neither significant epigenome-wide single CpG sites nor regions associated with impulsive and callous traits; the top-hits from these analyses were annotated to genes involved in neurotransmitter release and the regulation of the biological clock. No link to genetic variation was observed for these findings, which thus might reflect environmental influences. In conclusion, in this pilot study with a small sample size, we observed several DNA-methylation–disorder/trait associations of potential significance for ADHD and the related behavioral traits. Although we do not wish to draw conclusions before replication in larger, independent samples, cholesterol signaling and metabolism may be of relevance for the onset and/or persistence of ADHD.
Osteoimmunology: A Current Update of the Interplay Between Bone and the Immune System

Immunology, already a discipline in its own right, has become a major part of many different medical fields. However, its relationship to orthopedics and trauma surgery has unfortunately, and perhaps unjustly, been developing rather slowly. Discoveries in recent years have emphasized the immense breadth of communication and connection between both systems and, importantly, the highly promising therapeutic opportunities. Recent discoveries of factors originally assigned to the immune system have now also been shown to have a significant impact on bone health and disease, which has greatly changed how we approach treatment of bone pathologies. In case of bone fracture, immune cells, especially macrophages, are present throughout the whole healing process, assure defense against pathogens and discharge a complex variety of effectors to regulate bone modeling. In rheumatoid arthritis and osteoporosis, the immune system contributes to the formation of the pathological and chronic conditions. Fascinatingly, prosthesis failure is not at all solely a mechanical problem of improper strain but works in conjunction with an active contribution of the immune system as a reaction to irritant debris from material wear. Unraveling conjoined mechanisms of the immune and osseous systems heralds therapeutic possibilities for ailments of both. Contemplation of the bone as merely an unchanging support pillar is outdated and obsolete. Instead it is mandatory that this highly diverse network be incorporated in our understanding of the immune system and hematopoiesis.
Long-Term Fishing Catch and Effort Trends in the Republic of the Marshall Islands, With Emphasis on the Small-Scale Sectors

Small-scale fishing has been an important element of the livelihood and food security in Pacific island countries throughout history; however, such catches have been under-reported in the official fisheries data. Here, we reconstruct the total domestic catches and fishing effort of the Republic of the Marshall Islands (RMI) by fishing sectors for 1950–2017. Reconstructed total catches were estimated to be 27% higher than the data officially reported by the Food and Agriculture Organization (FAO) of the United Nations on behalf of the RMI. Catches of the truly domestic, but export-oriented, industrial tuna sector accounted for 84% of the total catch, dominating catches since the early 2000s. The subsistence component contributed 74% of total small-scale catches, of which 92% was deemed unreported. The remaining 26% of small-scale catches were artisanal, i.e., small-scale commercial, in nature, of which 45% was deemed unreported. Trends suggested steady growth in small-scale catches from 1,100 t⋅year–1 in the early 1950s to a relatively stable level of 4,500 t⋅year–1 since the 1990s. However, over the 2009–2017 period, there was a gradual reduction of 2% per year in subsistence fishing, which was paralleled by a concomitant increase in artisanal catches of 3% per year. This gradual shift from predominantly non-commercial to commercial small-scale fisheries may be related to efforts to commercialize small-scale fisheries in the past decades. Small-scale fishing effort increased approximately 13-fold from the early 1980s to the late 2000s, stabilizing at around 401,000 kWdays since then, while catch-per-unit-of-effort (CPUE) displayed an inverse pattern, declining eightfold between the 1980s and 1990s, and stabilizing around 15 kg⋅kWdays–1 in recent decades. These findings may assist sustainable coastal fisheries management in the RMI, which is particularly important given the increasing impacts of climate change on local stocks.
Oxytocinergic Cells of the Hypothalamic Paraventricular Nucleus Are Involved in Food Entrainment

When food is presented at a specific time of day subjects develop intense locomotor behavior before food presentation, termed food anticipatory activity (FAA). Metabolic and hormonal parameters, as well as neural structures also shift their rhythm according to mealtime. Food-entrained activity rhythms are thought to be driven by a distributed system of central and peripheral oscillators sensitive to food cues, but it is not well understood how they are organized for the expression of FAA. The hormone Oxytocin plays an important role in food intake, satiety and homeostatic glucose metabolism and although it is recognized that food is the main cue for food entrainment this hormone has not been implicated in FAA. Here we investigated the activity of oxytocinergic (OTergic) cells of the hypothalamus in relation to the timing of feeding in rabbit pups, a natural model of food entrainment. We found that OTergic cells of the supraoptic nucleus and the main body of the paraventricular nucleus (PVN) are activated after feeding which suggests that OT may be an entraining signal for food synchronization. Moreover, a detailed analysis of the PVN revealed that OTergic cells of the caudal PVN and a subpopulation in the dorsal part of the main body of this nucleus shows activation before the time of food but not 12 h later. Moreover this pattern persists in fasted subjects at the time of the previous scheduled time of nursing. The fact that those OTergic cells of the dorsal and caudal part of the PVN contain preautonomic cells that project to the adrenal, pancreas and liver perhaps may be related to the physiological changes in preparation for food ingestion, and synchronization of peripheral oscillators, which remains to be determined; perhaps they play a main role in the central oscillatory mechanism of FAA as their activity persists in fasted subjects at the time of the next feeding time.
NK Cell Priming From Endogenous Homeostatic Signals Is Modulated by CIS

Natural killer (NK) cell activation is controlled by a balance of activating and inhibitory signals and cytokines such as IL-15. We previously identified cytokine-inducible SH2-containing protein (CIS) as a negative regulator of IL-15 signaling in NK cells under inflammatory conditions. While the functional effect of Cish-deficiency in NK cells was obvious by their increased anti-tumor immunity and hyper-proliferative response to IL-15, it remained unclear how CIS regulates NK cell biology in steady-state. Here, we investigated the role of CIS in the homeostatic maintenance of NK cells and found CIS-ablation promoted terminal differentiation of NK cells and increased turnover, suggesting that under steady-state conditions, CIS plays a role in maintaining IL-15 driven regulation of NK cells in vivo. However, hyper-responsiveness to IL-15 did not manifest in NK cell accumulation, even when the essential NK cell apoptosis mediator, Bcl2l11 (BIM) was deleted in addition to Cish. Instead, loss of CIS conferred a lower activation threshold, evidenced by augmented functionality on a per cell basis both in vitro and in vivo without prior priming. We conclude that Cish regulates IL-15 signaling in NK cells in vivo, and through the rewiring of several activation pathways leads to a reduction in activation threshold, decreasing the requirement for priming and improving NK cell anti-tumor function. Furthermore, this study highlights the tight regulation of NK cell homeostasis by several pathways which prevent NK cell accumulation when IL-15 signaling and intrinsic apoptosis are dysregulated.
A Report on the Workshop Held at USDA's Economic Research Service

Since the mid-1970s, the prevalence of obesity and overweight has increased dramatically in the United States. The prevalence of overweight has tripled among children and adolescents, and nearly two out of three adult Americans are either overweight or obese. Although high health, social, and economic costs are known to be associated with obesity, the underlying causes of weight gain are less understood. At a basic level, weight gain and obesity are the result of individual choices. Consequently, economics, as a discipline that studies how individuals use limited resources to attain alternative ends, can provide unique insight into the actions and forces that cause individuals to gain excessive weight. In April 2003, USDA’s Economic Research Service and the University of Chicago’s Irving B. Harris Graduate School of Public Policy Studies and the George J. Stigler Center for the Study of the Economy and the State jointly hosted a workshop on the Economics of Obesity. The purpose was to provide an overview of leading health economics research on the causes and consequences of rising obesity in the United States. Topics included the role of technological change in explaining both the long- and short-term trends in obesity, the role of maternal employment in child obesity, the impact of obesity on wages and health insurance, behavioral economics as applied to obesity, and the challenges in measuring energy intakes and physical activity. The workshop also discussed policy implications and future directions for obesity research. This report presents a summary of the papers and the discussions presented at the workshop.
Decreased Hemoglobin Concentration and Iron Metabolism Disorder in Periodontitis: Systematic Review and Meta-Analysis

Background: Periodontitis is a chronic inflammatory disease with a possible infectious component. Anemia of inflammation (AI) occurring in various chronic diseases alters the hemoglobin (Hb) concentration and iron status. Currently, the association between periodontitis and AI is still controversial. The aim of this study was to assess the alterations of the level of hematological parameters and iron metabolism markers in patients with or without periodontitis. Methods: Electronic databases (MEDLINE, EMBASE, and Cochrane) were searched to identify publications about anemia and periodontitis. Subgroup analyses regarding gender, extent of periodontitis, and sample size were performed using STATA 12.1. Results: Sixteen studies were included in this meta-analysis. Pooled results showed a decrease in Hb [standardized mean difference (SMD) = −0.76, 95% CI = (−1.15, −0.37)], red blood cell [SMD = −0.69, 95% CI = (−1.09, −0.29)], hematocrit [SMD = −1.13, 95% CI = (−1.69, −0.57)], mean corpuscular volume [SMD = −0.16, 95% CI = (−0.32, −0.01)], and mean corpuscular Hb [SMD = −0.16, 95% CI = (−0.28, −0.04)], but upregulation in erythrocyte sedimentation rate [SMD = 0.63, 95% CI = (0.06, 1.19)]. In addition, patients with periodontitis had a higher level of hepcidin [SMD = 0.59, CI = (0.05, 1.12)] and decreased level of transferrin [SMD = −4.6, CI = (−13.1, −3.90)], with high heterogeneity. Conclusion: This meta-analysis indicates that periodontitis decreases Hb concentration and disturbs the balance of iron metabolism, which confirms strength of association between periodontitis and the development tendency of AI, especially for severe periodontitis. More unbiased cohort studies with larger sample sizes are still warranted to make a definitive judgment in the future.
Pathological ATPergic Signaling in Major Depression and Bipolar Disorder

The mood disorders, major depression (MD) and bipolar disorder (BD), have a high lifetime prevalence in the human population and accordingly generate huge costs for health care. Efficient, rapidly acting, and side-effect-free pharmaceuticals are hitherto not available, and therefore, the identification of new therapeutic targets is an imperative task for (pre)clinical research. Such a target may be the purinergic P2X7 receptor (P2X7R), which is localized in the central nervous system (CNS) at microglial and neuroglial cells mediating neuroinflammation. MD and BD are due to neuroinflammation caused in the first line by the release of the pro-inflammatory cytokine interleukin-1β (IL-1β) from the microglia. IL-1β in turn induces the secretion of corticotropin-releasing hormone (CRH) and in consequence the secretion of adrenocorticotropic hormone (ACTH) and cortisol, which together with a plethora of further cytokines/chemokines lead to mood disorders. A number of biochemical/molecular biological measurements including the use of P2X7R- or IL-1β-deficient mice confirmed this chain of events. More recent studies showed that a decrease in the astrocytic release of ATP in the prefrontal cortex and hippocampus is a major cause of mood disorders. It is an attractive hypothesis that compensatory increases in P2X7Rs in these areas of the brain are the immediate actuators of MD and BD. Hence, blood-brain barrier-permeable P2X7R antagonists may be promising therapeutic tools to improve depressive disorders in humans.
The Influence of Empathy Trait and Gender on Empathic Responses. A Study With Dynamic Emotional Stimulus and Eye Movement Recordings

Previous studies have suggested that empathic process involve several components such as cognitive empathy, affective empathy, and prosocial concern. It has also been reported that gender and empathy trait can influence empathic responses such as emotional recognition, which requires an appropriate scanning of faces. However, the degree to which these factors influence the empathic responses, which include emotion recognition, affective empathy, and cognitive empathy, has not yet been specified. Aim: The aim of the present study was to identify the differences between individuals with high and low level of empathy trait, as well as differences between men and women, in an explicit task in order to evaluate the empathic responses. Methods: With this goal in mind, we recorded eye movements during the presentation of dynamic emotional stimuli (joy, anger, fear, and neutral videos). After watching each video, participants had to rate the valence and arousal dimensions of emotional content and explicit empathy responses were assessed. Thirty participants (15 women) were included in a High Empathy group (HE; mean age = 21.0) and 30 participants (16 women) in the Low Empathy group (LE; mean age = 21.2), according to their scores in the Interpersonal Reactivity Index (IRI) scale. Results: As expected, the HE group showed higher scores than the LE group in the explicit empathy responses. These differences, based on global scores, were mainly explained by affective empathy and cognitive empathy responses but not by emotional recognition one. No differences were observed by gender in these measures. Regarding eye movements in the dynamic emotional stimuli, HE group had longer fixation duration on the eyes area than LE group. In addition, women spent more time on the eyes area in comparison to men. Discussion: Our findings suggest that both men and women with high empathy trait are more accurate to empathizing but not on the basis of the emotional recognition response. The fact that women spent more time on the eyes area did not seem to affect the empathic responses to the dynamic emotional stimulus. Overall, empathic responses of both men and women are modulated by their empathic trait. In addition, empathic trait and gender seem to impact strategies to deal with emotional facial information.