Miracle-dz/UniFAR-FTUs huggingface dataset:
Specific function of STAT3, SOCS1, and SOCS3 in the regulation of proliferation and survival of classical Hodgkin lymphoma cells

The essential regulators in the pathogenesis of classical Hodgkin lymphoma (cHL) are still largely unknown. The malignant Hodgkin/Reed–Sternberg (HRS) cells of cHL secrete various cytokines leading to the activation of signaling pathways such as the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway. In this study, we investigate the role of distinct JAK/STAT pathway components in the regulation of proliferation and survival of cHL cell lines. Electrophoretic mobility shift assay and western blot analysis revealed that the activation status of STAT family members varies in different cHL cell lines. Tyrosine kinase inhibitors of the JAK/STAT pathway blocked the activation of most of the STAT family members. This was accompanied with a strong antiproliferative effect and enhanced death of the treated cHL cell lines. Specific downregulation of STAT3 by siRNA expression decreased cell proliferation and induced apoptosis. Overexpression of SOCS1 and SOCS3 resulted in a proliferation arrest of cells with limited endogenous amount of these negative regulators, but not in cells that already express high amounts of SOCS1 and SOCS3. Our findings highlight the importance of STAT3 in cHL transformation and suggest SOCS1 and SOCS3 as potential targets for therapeutic intervention in distinct forms of cHL. © 2005 Wiley‐Liss, Inc.
Reducing Data Copying Overhead in Web Servers

Web servers that generate dynamic content are widely used in the development of Internet applications. With the Internet highly connected to people’s lifestyles, the service requirements of Internet applications have increased significantly. This increasing trend intensifies the need to improve server performance in dynamic content generation. In this thesis, we describe the opportunity to improve server performance by colocating the web server and the application server on the same machine. We identify related work and discuss their respective advantages and deficiencies. We then introduce and explain our technique that passes the client socket’s file descriptor from the web server process to the application server. This allows the application server to reply to the client directly, reducing the amount of data copied and improving server performance. Experiments were designed to evaluate the performance of this technique and provide a detailed analysis of processor time and data copying during response delivery. A performance comparison against alternative approaches has been performed. We analyze the results to understand factors in data copying efficiency and determine that cache misses are an important factor in server performance. There are four major contributions in this thesis. First, we show that in multiprocessor environments, co-locating web servers and application servers can take advantage of faster communication. Second, we introduce a new technique that reduces the amount of data copied by two-thirds. This technique requires no modifications to the application server code (other existing techniques do), and it is also applicable in a variety of systems, allowing easy adoption in production environments. Third, we provide a performance comparison against other approaches and raise questions regarding data copying efficiency. Our technique attains an average peak throughput of 1.27 times the FastCGI with Unix domain sockets in both uniprocessor and multiprocessor environments. Finally, our analysis on the effect of cache misses on server performance provides valuable insights into why these benefits are obtained.
Diagnosis of Trichomonas vaginalis infection.

To the Editor. — In the QUESTIONS AND ANSWERS section ofThe Journal(241: 1176, 1979), E. Stewart Taylor, MD, recommended treatment of asymptomatic women with evidence of Trichomonas vaginalis on their Papanicolaou smears. It is my belief that treatment should be reserved for those patients in whom infection is confirmed after careful examination and wet smear. Although a few cytological laboratories may be capable of identifying trichomonal infection when present, the majority may not be sufficiently accurate to warrant treatment on the basis of the Papanicolaou smear alone. 1-5 In one study of 666 women with evidence of T vaginalis on their Papanicolaou smears, there was no evidence of the organism on wet smear or culture in 246 (37%) of these patients. Before a physician treats a patient and her consort for T vaginalis infection simply on the basis of her Papanicolaou smear, he should first investigate the accuracy of
Lipoprotein lipase correlates positively and hepatic lipase inversely with calcific atherosclerosis in homozygous familial hypercholesterolemia.

Homozygous familial hypercholesterolemia (FH) is a rare genetic disorder that leads to premature atherosclerosis due to a defective LDL receptor. There is, however, a large degree of phenotypic heterogeneity at the level of atherosclerosis even in patients with identical mutations of the LDL receptor protein. Lipoprotein lipase (LPL) and hepatic lipase (HL) are crucial enzymes in lipoprotein metabolism, and both have been proposed as having proatherogenic as well as antiatherogenic effects. To evaluate a potential role for these enzymes in the severity of atherosclerosis, we correlated postheparin LPL mass and activity as well as HL activity with the volume of total calcific atherosclerosis (heart and thoracic aorta), coronary artery calcific atherosclerosis, and Achilles tendon width as measured by computed tomography in 15 FH homozygotes. LPL dimer and total mass were positively correlated with all three parameters (r = .65 to .87, P < .01) as was LPL activity (r = .52 to .63, P < .05). HL activity was negatively correlated with total and coronary artery calcified lesion volume (r = -.55 to .57, P < .05). In a multiple regression model of the coronary artery lesion volume, LPL dimer mass and HL activity together accounted for 84% of the variability (r = .92, P < .0001). In a multiple regression model of the total calcified lesion volume, HL activity, total cholesterol, age, and LPL dimer mass together accounted for 85% of the variability (r = .92, P = .0005). These data demonstrate a significant correlation of LPL mass and activity with the extent of calcific atherosclerosis in homozygous FH. It is not clear whether LPL is the cause or consequence of the observed correlation, but if the association between LPL and coronary artery lesions is also present in patients with other genetic dyslipoproteinemias, LPL could constitute a new risk factor for cardiovascular disease.
Complement Lysis: the Ultrastructure and Orientation of the C5b‐9 Complex on Target Sheep Erythrocyte Membranes

The C5b‐9 complex derived from human serum and assembled on target sheep erythrocyte membranes is a thin‐walled cylinder rimmed by an annulus at one end. The total height of the cylinder is 150 Å, towards which the annulus contributes 30 Å. The cylinder has an apparently uniform internal diameter of 100 Å. The external diameter of the annulus is 200 Å. The classical complement ‘rings’ visualized on membranes after complement lysis represent such C5b‐9 cylinders perpendicularly oriented on the membranes. The thin‐walled cylinder is anchored in the membrane maim and the annulus located in the exterior membrane glycocalyx. At the sites of attachment of the C5b‐9 complexes, the continuity of the membrane bilayer is disturbed and the presence of trans‐membrane pores is indicated The data essentially support the ‘doughnut’ theory of complement lysis.
Critères numériques et temporels pour la détection de documents vitaux dans un flux

This paper addresses to a classification challenge in a filtering task. We use different kind of features to depict vital documents and filter them out from the stream. A vital document has to be relevant for a particular entity and has to relate a new story about it. We introduce different features that uses time as well as entity profil to perform classification. We evaluate our method on framework from TREC KBA 2013 (Knowledge Base Acceleration). MOTS-CLES : Filtrage, modele adaptatif, profil d’entite, Random Forest,
Broiler lines divergently selected for digestive efficiency also differ in their susceptibility to colibacillosis

Increasing feed efficiency of broiler chickens by selective breeding could lead to decreased feed cost and reduced environmental impact of poultry production. At INRA, two broiler chicken lines (D+/D−) were divergently selected for their digestive efficiency. Strong differences were shown between both lines for the anatomy and histology of the digestive tract, and for the intestinal microbiota composition. In the present study, we investigated whether this selection also had an effect on susceptibility to colibacillosis, which is one of the main causes of economic losses in poultry production. The broiler lines D+/D− were challenged with an avian pathogenic Escherichia coli strain. A first experiment was conducted to assess the 50% lethal dose by subcutaneous infection of hatchlings, whereas a second experiment reproduced colibacillosis by infecting air sacs of 23-day-old chicks. The 50% lethal dose was very low for both lines. However, the line with the higher digestive efficiency (D+) was the less susceptible to colibacillosis. This result is interesting for selection purposes and opens the way to integrative genetic studies of the interactions between digestion efficiency and resistance to colibacillosis.
Use of a hyperbaric solution for administration of intrathecal amphotericin B.

Abstract Repeated intrathecal administration of amphotericin B is often used in treatment of patients with fungal meningitis, but cisternal injections are hazardous and lumbar injection may cause local arachnoiditis. To study the distribution of hyperbaric solutions of amphotericin in 10 per cent dextrose in water, such solutions mixed with 131I albumin were injected into the lumbar space of 21 monkeys, and external radioactivity was monitored to estimate the amount of injected material that reached the cisterna magna. Higher cisternal concentrations of radioisotope were found in the 12 monkeys that were injected with hyperbaric material and then placed in the Trendelenburg position than in nine monkeys that were supine and given non-hyperbaric amphotericin solutions. This technic may prove useful in treatment of fungal meningitis. (N Engl J Med 290:641–646, 1974)
Vehicle detection from video sequence based on gabor filter

Detecting vehicles from video sequence is very challenging due to the wide varieties of vehicle appearances and the complexity of the backgrounds. At present, many algorithms in the image recognition have a narrow applicability and a weak real-time. Aiming at this problem, a recognition method which was combined by features extraction using Gabor wavelet and BP neural network algorithm for the classification of vehicle types based on the texture model is proposed. Firstly, the background image is captured and automatically updated. Moving vehicles is detected and abstracted by background subtraction. For removing the noise, the images were filtered by median filtering. Secondly, a model of feature vector based on Gabor filter is built. At last, BP neural network classifier was designed to train and identify the feature points. Experiments show that this approach has better robustness, good real-time and high recognition rate, with a wide range of practical applications.
Linking the MIS Plan with Corporate Strategy: An Exploratory Study

In recent years there has been an increasing amount of attention paid to effective strategic planning of the information systems resource in many organizations. The motivation for this attention derives from a number of resources, especially the rapid rate of technological progress in computer and telecommunications facilities to problem domains that are critical to organizational success. As a wide range of new opportunities become apparent, it has become a more difficult to mate the operational, managerial, and strategic needs of the business with appropriate systems activities. Based upon an exploratory study of eight organizations which involved extensive interviews with IS and senior managers, this article describes the planning practices observed and identifies several factors which seemed particularly important to their planning success, or lack of it. These factors include such things as the style of senior management decision making, the volatility of the business and by extension, the applications development portfolio), the complexity of the IS organization and management task, and the status and physical location of the IS manager.
Spatial and Temporal Distribution of Residues of Imidacloprid and Its Insecticidal 5-Hydroxy and Olefin and Metabolites in Eastern Hemlock (Pinales: Pinaceae) in the Southern Appalachians

ABSTRACT Widespread mortality of eastern hemlock, Tsuga canadensis (L.) Carrière, resulting from infestation by hemlock woolly adelgid, Adelges tsugae Annand (Hemiptera: Adelgidae), has occurred throughout the native range of eastern hemlock within the eastern United States. Imida-cloprid, a systemic insecticide, is one of the primary chemical compounds used to control hemlock woolly adelgid in both urban and, in a limited manner, in natural forest environments. The metabolism of imidacloprid in eastern hemlock produces 12 metabolites; two of these, imidacloprid 5-hydroxy and imidacloprid olefin, are considered toxicologically important metabolites. However, little is known about the persistence of these metabolites in eastern hemlock in the southern Appalachians. Concentrations of imidacloprid, olefin, and 5-hydroxy were quantified by using HPLC/MS/MS techniques. Over the 3-yr study, concentrations of imidacloprid and consequent 5-hydroxy and olefin were highest in trees treated with a soil injection in the spring. Imidacloprid and 5-hydroxy concentrations in sap were highest at 12 mo posttreatment and in tissue at 15 mo posttreatment. Imidacloprid was detected through 36 mo posttreatment and 5-hydroxy was detected through 15 mo posttreatment. Olefin concentrations in both sap and tissue were highest at 36 mo posttreatment and were detected in high concentrations through 36 mo posttreatment. Concentrations of imidacloprid were highest in the bottom stratum of the canopy and lowest in the top stratum. Concentrations of olefin and 5-hydroxy were highest in the top stratum and lowest in the bottom stratum.
Interdisciplinary Rounds and Structured Communication Reduce Re-Admissions and Improve Some Patient Outcomes

Hospital communication is more than access to information. Among staff, it is about achieving situation awareness—an understanding of a patient’s current condition and likely trajectory. In the multidisciplinary context of providing care, structure, consistency, and repeatability of communication will enable a shared understanding of the patient and plan, leading to improved patient satisfaction and outcomes. This was tested using the Situation-Background-Assessment-Recommendation (SBAR) protocol, a re-admissions risk assessment and daily interdisciplinary rounds (IDR) in the medical/surgical units of a hospital. The impact of these interventions on patient satisfaction, Foley catheter removal compliance, and patient re-admission rates was assessed. Over the 3 year period, Foley compliance improved from 78% to 94%, and re-admissions decreased from 14.5% to 2.1%, both significant. Patient satisfaction trended positively, but was not significant. These results support the value of SBAR and IDR, and are advocated to improve situation awareness and maintain focus on key patient data.
Robust Variable Selection Methods for Grouped Data

When predictor variables possess an underlying grouping structure in multiple regression, selecting important groups of variables is an essential component of building a meaningful regression model. Some methods exist to perform group selection, but do not perform well when the data include outliers. Four methods for robust variable selection of grouped data, based on the group LASSO, are presented: two regular methods and two adaptive methods. For each of the two methods in the regular and adaptive groups, one method works well for data with outliers in the y-direction, and the other method works well for data with outliers in both the xand ydirections. The effectiveness of each of these methods is illustrated with an extensive simulation study and a real data example.
Functional and anatomic organization of three-dimensional eye movements in rabbit cerebellar flocculus.

1. The three-dimensional, binocular eye movements evoked by electrical microstimulation of the cerebellar flocculus of alert, pigmented rabbits were recorded using the scleral search coil technique. The components of these eye movements were obtained in reference to an orthogonal coordinate system consisting of a vertical axis and two horizontal axes at 45 degrees and 135 degrees azimuth. The azimuth coordinate was taken to increase to both sides from the 0 degrees reference in the direction of the nose. 2. Eye movements were evoked most readily by stimulation (0.2-ms pulses at 200 Hz for 1 s, intensity < or = 20 microA) at loci in the deep granular layer and the white matter. They consisted of slow (5-20 deg/s) movements. The responses were either binocular, with the eye ipsilateral to the stimulated flocculus usually having the larger amplitude, or were monocular, in which case they were restricted to the ipsilateral eye. 3. The evoked responses were classified according to the combination of the largest measured component of rotation for the two eyes and its sense of rotation (clockwise, CW, or counterclockwise, CCW). Seventy-eight percent of the evoked eye movements could be placed in one of two classes. For one of these classes the largest response component was a short-latency abduction of the ipsilateral eye about its vertical axis (19%), whereas for the other class (59%), the largest response component was a short-latency CCW rotation of the ipsilateral (left) eye about its 135 degrees axis. This response was frequently (50%) accompanied by a smaller short-latency CW rotation of the contralateral (right) eye about its 45 degrees axis. 4. The two main classes of three-dimensional eye movements are associated differentially with anatomically distinguishable compartments that are revealed by acetylcholinesterase histochemistry. Of the five anatomically distinguishable compartments in the floccular white matter, three are predominant. The middle of these three compartments is associated with the vertical axis class of movements, whereas the two adjacent compartments are associated with the 135 degrees class of eye movements. The eye movement relation of the other two, smaller compartments, was not determined. 5. The spatial orientation of the rotation axes of the two main classes of evoked eye movements closely corresponds to that of the preferred axes of the visual climbing fiber input to the flocculus. This suggests that both are organized in a similar coordinate system. (ABSTRACT TRUNCATED AT 400 WORDS)
Forecasting the Future of Cardiovascular Disease in the United States: A Policy Statement From the American Heart Association

Background— Cardiovascular disease (CVD) is the leading cause of death in the United States and is responsible for 17% of national health expenditures. As the population ages, these costs are expected to increase substantially. Methods and Results— To prepare for future cardiovascular care needs, the American Heart Association developed methodology to project future costs of care for hypertension, coronary heart disease, heart failure, stroke, and all other CVD from 2010 to 2030. This methodology avoided double counting of costs for patients with multiple cardiovascular conditions. By 2030, 40.5% of the US population is projected to have some form of CVD. Between 2010 and 2030, real (2008$) total direct medical costs of CVD are projected to triple, from $273 billion to $818 billion. Real indirect costs (due to lost productivity) for all CVD are estimated to increase from $172 billion in 2010 to $276 billion in 2030, an increase of 61%. Conclusions— These findings indicate CVD prevalence and costs are projected to increase substantially. Effective prevention strategies are needed if we are to limit the growing burden of CVD.
Probabilistic load flow of islanded microgrid with droop-controlled distributed generations

This paper proposes a method of the probabilistic load flow calculation of islanded microgrid (IMG) with droop-controlled distributed generations (DGs). The deterministic load flow is calculated based on Newton Raphson method considering the droop characteristics of DGs. The Monte Carlo method is applied for the evaluation of probabilistic distribution of load flow variables. The stochastic models of distributed generating systems including wind turbines and photovoltaic (PV) systems are established. The load model is considered as following a normal distribution. The effectiveness and efficiency of the proposed method are tested on a modified IEEE33 test system.
Simian virus 40 late transcripts lacking excisable intervening sequences are defective in both stability in the nucleus and transport to the cytoplasm

Little or no simian virus 40 (SV40) late mRNA accumulates in the cytoplasm when the primary transcript lacks an excisable intervening sequence. To begin to understand why, we analyzed the synthesis, processing, transport, and stability of SV40 late transcripts accumulated in the nucleus and cytoplasm of monkey cells cotransfected with the DNAs of wild-type and mutants of SV40 lacking precisely various introns. The data from these experiments indicated that (i) the presence of excisable intervening sequences in SV40 late transcripts is necessary for efficient accumulation in the cytoplasm of any of the SV40 late RNA species and (ii) SV40 late transcripts lacking excisable intervening sequences are defective in both stability in the nucleus and transport to the cytoplasm but not in stability in the cytoplasm. We hypothesize that SV40 late transcripts need to be processed via a pathway that couples stabilization of the primary transcript within the nucleus, excision of intervening sequences, proper 5'- and 3'-end formation, and transport to the cytoplasm.
PEM Fuel Cells Modeling and Analysis Through Current and Voltage Transient Behaviors

In the last 15 years, polymer electrolyte membrane (PEM) fuel cells have received much attention, mostly through experimental and empirical studies in scientific and industrial research. In most of the works, attention has been given to the steady state analysis of the PEM fuel cells. However, considerable efforts still need to be done to explain different transient behaviors of PEM fuel cells. This paper presents an analysis of the double layer charging effect and reactant diffusion through the cathode gas diffusion layer on voltage transients after sudden current variations. These transient phenomena have typical time durations of less than 5 s. The double layer charging dynamic explains the main voltage transient behaviors when the cathode inlet pressure is constant. In this case, a bicriteria optimization procedure is proposed for numerical characterization of the double-layer charging capacitance. When the air pressure is variable, a pseudo 2-D modeling of oxygen diffusion through the cathode gas diffusion layers, based on the Stephan-Maxwell multicomponent diffusion equations, is used to explain its contribution to the voltage transient overshoots/undershoots.