Miracle-dz/UniFAR-FTUs huggingface dataset:
Analysis of mismanaged plastic waste in Samoa to suggest proper waste management in Pacific island countries

Pacific island countries are facing critical challenges in managing the growing amount of increasingly diverse wastes because of changing lifestyles and the concentration of populations in urban areas. Although marine plastic waste is an issue in the Pacific region, there have been almost no studies of the estimation and impacts of ocean plastic waste, and measures to control such waste, in these countries. Here, first we conducted a questionnaire survey about consumption and disposal of plastic products at households in Samoa as one of the examples of Pacific island countries to grasp the flow of plastic materials and awareness or behaviour for plastic waste. Then we quantified ocean plastic waste and discussed the effective and needed countermeasures in Pacific island countries. The total amount of mismanaged plastic waste was estimated to be 327,000 or 156,000 t y−1 (depending on the scenario) in Pacific island countries. The regional Pacific island countries contribution to the global total mismanaged plastic waste was estimated to range from 1.3% to 2.7%. The amount of mismanaged plastic waste per capita in some Pacific island countries, such as Solomon Islands and Micronesia, was also ranked highly globally. The main reasons seem to be that waste collection rates are relatively low in rural areas and rates of plastic waste generation are high. To implement solutions, including improving municipal solid waste collection, reducing plastic waste, improving the collection and recycling of plastics, and the integration of a number of policies is essential.
Health-promoting properties of oleocanthal and oleacein: Two secoiridoids from extra-virgin olive oil

Abstract Extra virgin olive oil (EVOO) polyphenols, including the secoiridoids oleocanthal (OLC) and oleacein (OLE), are attracting attention because of their beneficial effects on health. Data on OLC and OLE bioavailability are scarce, as most research on EVOO polyphenols has concentrated on hydroxytyrosol, tyrosol, and oleuropein. Consequently, relevant goals for future research are the elucidation of OLC and OLE bioavailability and finding evidence for their beneficial effects through pre-clinical and clinical studies. The aim of this review is to shed light on OLC and OLE, focusing on their precursors in the olive fruit and the impact of agronomic and processing factors on their presence in EVOO. Also discussed are their bioavailability and absorption, and finally, their bioactivity and health-promoting properties.
Repeated exposure to exemplars does not enhance implicit learning: A puzzle for models of learning and memory

We learn regularities in the world around us, frequently without conscious effort, a phenomenon known as implicit learning. These regularities are often impossible to verbalise. One example of implicit learning is the structural effect, in which participants learn a rule set combining two factors, such as lexical frequency and concreteness. Theories of implicit learning predict that repetition of exemplar words would result in improved learning of the rule set, increasing the magnitude of the structural effect. Over four experiments, we demonstrate that this is, in fact, not the case. In Experiments 1 and 2, three repetitions of exemplar words result in superior item memory, but no change in the magnitude of the structural effect, compared with individually presented words. In Experiments 3 and 4, the structural effect is shown to be invariant to five repetitions of exemplar words and at high and low numbers of exemplars. In all four experiments, participants were unable to describe the rule set underlying the structural effect. However, confidence ratings demonstrated sensitivity to the structure and this sensitivity, unlike endorsements, increased with strength. The results are discussed in reference to differentiation, structural versus judgement knowledge, and flexible learning systems.
Achievement of glycaemic control is associated with improvements in lipid profile with iGlarLixi versus iGlar: A post hoc analysis of the LixiLan‐L trial

Diabetic dyslipidaemia is a major risk factor for accelerated atherosclerosis. Glycaemic treatments that improve dyslipidaemia may help reduce the burden of atherosclerosis. This analysis investigated the effect of iGlarLixi [insulin glargine U100 (iGlar) and lixisenatide] versus iGlar on lipid profiles in patients with type 2 diabetes uncontrolled on basal insulin. Data from LixiLan‐L were used to estimate changes in fasting lipid levels from baseline to week 30, overall and in patients stratified by achievement of glycaemic targets {2‐hour postprandial glucose [≤10, >10 mmoL/L], fasting plasma glucose [≤6.1, >6.1 mmoL/L], HbA1c [≤7, >7% (≤53, >53 mmol/mol)]}. At week 30, median percentage change in triglycerides remained nearly unchanged (0.3% increase) with iGlarLixi versus a 6.5% increase with iGlar (P = 0.035; overall); similarly, trends towards better total and LDL cholesterol levels were observed with iGlarLixi versus iGlar. In patient subgroups achieving glycaemic targets, all lipid variables except for HDL cholesterol improved with iGlarLixi but not with iGlar. In summary, patients with type 2 diabetes uncontrolled on basal insulin showed improved fasting lipid profiles with iGlarLixi compared with iGlar, particularly when achieving glycaemic targets.
The paradox of obligate sex: The roles of sexual conflict and mate scarcity in transitions to facultative and obligate asexuality

The maintenance of obligate sex in animals is a long‐standing evolutionary paradox. To solve this puzzle, evolutionary models need to explain why obligately sexual populations consistently resist invasion by facultative strategies that combine the benefits of both sexual and asexual reproduction. Sexual antagonism and mate availability are thought to shape the occurrence of reproductive modes in facultative systems. But it is unclear how such factors interact with each other to influence facultative invasions and transitions to obligate asexuality. Using individual‐based models, we clarify how sexually antagonistic coevolution and mate availability affect the likelihood that a mutant allele that gives virgin females the ability to reproduce parthenogenetically will invade an obligately sexual population. We show that male coercion cannot stop the allele from spreading because mutants generally benefit by producing at least some offspring asexually prior to encountering males. We find that effects of sexual conflict can lead to positive frequency‐dependent dynamics, where the spread of the allele is promoted by effective (no‐cost) resistance when males are common, and by mate limitation when sex ratios are female‐biased. However, once the mutant allele fixes, effective coercion prevents the complete loss of sex unless linkage disequilibrium can build up between the allele and alleles for effective resistance. Our findings clarify how limitations of female resistance imposed by the genetic architecture of sexual antagonism can promote the maintenance of sexual reproduction. At the same time, our finding of widespread obligate sex when costs of parthenogenesis are high suggests that developmental constraints could contribute to the rarity of facultative reproductive strategies in nature.
Effects of motorcycle simulator configurations on steering control and gaze behavior in bends.

The recent development of motorcycle simulators has made it possible to study rider behavior in safe conditions. However, their use still raises validity issues. Our study examined how riders' steering and gaze behaviors and subjective experience are influenced by motorcycle roll tilt and reverse steering, which are considered to be essential factors in real-life motorcycle riding. The results revealed that tilting the motorcycle in the roll plane did not lead to significant changes in rider behavior, gaze sampling, or perceived realism. The steering control strategy adopted by riders did, however, significantly influence these results. A direct steering control strategy meant that riders took a racing path and scanned the road far in advance. When reverse steering was implemented, however, riders chose to take a "safety path", as recommended by training manuals. Reverse steering also received the highest realism score. However, steering control was more difficult, as shown by the larger number of lane departures recorded and a change in the trade-off between guiding and look-ahead fixations. This suggests that although reverse steering matches riders' real control behavior and improves the subjective experience of simulator riding, it is hindered by an inadequate internal model of vehicle dynamics. (PsycINFO Database Record (c) 2019 APA, all rights reserved).
In tube immunocytochemistry for fluorescence-activated cell sorting that prevents RNA degradation in sorted cells

ABSTRACT Fluorescence-activated cell sorting (FACS) is a powerful tool for analyzing stem cells. When using fixed cells, however, it is sometimes difficult to analyze RNA extracted from sorted cells due to RNA degradation. We established a protocol for immunocytochemistry before FACS to prevent RNA degradation. Cells were fixed with a methanol-based fixative (UM-Fix), then subjected to immunocytochemistry. The addition of RNase inhibitor and dithiothreitol (DTT) to some buffers used for immunocytochemistry increased RNA integrity after cell recovery. We found increased copy numbers of mRNA in recovered cells using quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. When RNase inhibitor and DTT were added, amplification of mRNA using T7 promoter was possible with RNA extracted from recovered cells after FACS. Our protocol ensures high quality RNA in cells recovered by FACS; therefore, gene expression analysis with a smaller number of cells is possible using pre-amplification of mRNAs. Our protocol for immunocytochemistry also might be applicable to RNA recovery after immunostaining.
A Case of Transcranial Direct-Current Stimulation for Childhood Stroke Hemiparesis: A Brief Report

ABSTRACT Purpose: Survivors of childhood stroke incur lifelong physical disability. Treatment options are limited, however, models of motor reorganization after stroke are revealing cortical targets for neuromodulation. Transcranial direct-current stimulation (tDCS) enhances motor learning and may improve motor recovery in adult stroke, but remains uninvestigated in childhood-onset stroke. Here we documented the feasibility and safety of tDCS in an adolescent with chronic stroke-induced hemiparesis. Materials and methods: Over 10 days, the participant underwent occupational therapy paired with contralesional, primary motor cortex-targeting, cathodal tDCS. Clinical motor outcomes, and safety and tolerability measures were completed. Results: tDCS was well-tolerated with no adverse events. Motor outcomes did not regress post-intervention, with clinically significant changes still evident at 6 months. Conclusions: Application of controlled trials of non-invasive neuromodulation are safe and tolerability in childhood-onset stroke.
Ultra‐early continuous cardiac monitoring improves atrial fibrillation detection and prognosis of patients with cryptogenic stroke

Subclinical atrial fibrillation (AF) is known to underlie a number of cases of cryptogenic stroke (CrS). However, there is need to define the most effective strategy for AF detection. The diagnostic usefulness was analysed of a strategy based on ultra‐early continuous monitoring in patients with CrS in terms of AF detection, oral anticoagulation treatment and stroke recurrence, in comparison to a standard outpatient strategy.
Association Between Maternal Fluoride Exposure During Pregnancy and IQ Scores in Offspring in Canada

Key Points Question Is maternal fluoride exposure during pregnancy associated with childhood IQ in a Canadian cohort receiving optimally fluoridated water? Findings In this prospective birth cohort study, fluoride exposure during pregnancy was associated with lower IQ scores in children aged 3 to 4 years. Meaning Fluoride exposure during pregnancy may be associated with adverse effects on child intellectual development, indicating the possible need to reduce fluoride intake during pregnancy.
A hypomorphic allele of SLC35D1 results in Schneckenbecken-like dysplasia

Abstract We report the case of a consanguineous couple who lost four pregnancies associated with skeletal dysplasia. Radiological examination of one fetus was inconclusive. Parental exome sequencing showed that both parents were heterozygous for a novel missense variant, p.(Pro133Leu), in the SLC35D1 gene encoding a nucleotide sugar transporter. The affected fetus was homozygous for the variant. The radiological features were reviewed, and being similar, but atypical, the phenotype was classified as a ‘Schneckenbecken-like dysplasia. ’ The effect of the missense change was assessed using protein modelling techniques and indicated alterations in the mouth of the solute channel. A detailed biochemical investigation of SLC35D1 transport function and that of the missense variant p.(Pro133Leu) revealed that SLC35D1 acts as a general UDP-sugar transporter and that the p.(Pro133Leu) mutation resulted in a significant decrease in transport activity. The reduced transport activity observed for p.(Pro133Leu) was contrasted with in vitro activity for SLC35D1 p.(Thr65Pro), the loss-of-function mutation was associated with Schneckenbecken dysplasia. The functional classification of SLC35D1 as a general nucleotide sugar transporter of the endoplasmic reticulum suggests an expanded role for this transporter beyond chondroitin sulfate biosynthesis to a variety of important glycosylation reactions occurring in the endoplasmic reticulum.
Spontaneous Rupture and Entanglement of Human Neuronal Tau Protein Induced by picoNewton Compressive Force.

Mechanical force vector fluctuations in living cells can have significant impact on protein behavior and functions. Here we report that a human Tau-protein tertiary structure can abruptly and spontaneously rupture, like a balloon, under biologically-available picoNewton compressive force, using a home-modified Atomic Force Microscopy single-molecule manipulation. The rupture behavior is dependent on the physiological level of presence of ions, such as K+ and Mg++. We observed rupture events in the presence of K+ but not in the presence of Mg2+ ions. We have also explored the entangled protein state formed following the events of the multiple and simultaneous protein ruptures under crowding. Crowded proteins simultaneously rupture and then spontaneously refold to an entangled folding state, different from either folded and unfolded states of the Tau protein, can be a plausible pathway for the tau protein aggregation that are related to a number of neuronal degenerated diseases.
How Does Perceived Health Status Affect Depression in Older Adults? Roles of Attitude toward Aging and Social Support

ABSTRACT Objectives: This study aims to test the mediating effect of attitude toward aging and the moderating effect of social support in the association between perceived health status and depression in older adults. Methods: The study hypotheses were tested by using quantitative data collected from 6485 Chinese older adults. SPSS macro PROCESS was employed to analyze the data. Results: The results show that the relationship between perceived health status and depression in older adults is mediated by their attitude toward aging. In addition, both direct and indirect impacts of perceived health status on depression are significantly buffered by social support. Conclusions: This study suggests a potential mediating role of attitude toward aging and a moderating role of social support in the association between physical health and mental health among older adults. Clinical Implications: The findings of the study suggest that clinicians might need to consider attitudes toward aging along with social support as they work with older adults with comorbid physical illnesses and depression. The findings also reveal that social support is not only about the external resources that older adults need to cope with stressful life events, but also serves as an inner sustenance to make older adults less sensitive to the deleterious effects of poor health status and negative attitude toward aging.
Exposure of BPA and its alternatives like BPB, BPF, and BPS impair subsequent reproductive potentials in adult female Sprague Dawley rats

Abstract Bisphenol A (BPA) and its alternatives are extensively used in household and industrial products. BPA and its alternatives have affinity for estrogen receptors and mimic its actions. The present study aims to examine the comparative effects of BPA and its alternatives bisphenol B (BPB), bisphenol F (BPF), and bisphenol S (BPS) on the reproductive health in adult female rats. One hundred and seventy post-weaning female rats (90 ± 25) were divided into 17 groups and assigned for different treatments. Control and treated groups were injected with concentrations of 50–500 µg/ml and 5–50 mg/kg of BPA, BPB, BPF, and BPS for 28 days. The results showed adverse morphological and histopathological alterations in rat ovaries in all treated groups. A remarkable decrease was observed in antral and corpus luteum follicles while rise in atretic and cystic follicles in the ovaries of BPA and its alternatives 5 and 50 mg/kg treated groups when compared with control. Significant decrease in catalase (CAT), super oxidase (SOD), and peroxidase (POD) levels was noted while increase in the values of thiobarbituric acid reactive substance (T-BARS) and reactive oxygen species (ROS) was observed when BPA and its alternatives groups were compared with the control. Hormones were also observed with alterations in their concentrations when treated groups were compared with the control. The current data suggest that BPA and its alternatives exposure during the pre-pubertal stage have the potential to induce oxidative stress and histopathological changes during follicular development in female rats.
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Active fault databases are a very powerful and useful tool in seismic hazard assessment, particularly when singular faults are considered as seismogenic sources. Active fault databases are also a very relevant source of information for earth scientists, earthquake engineers and even teachers or journalists. Active fault databases, hence, should be updated and through reviewed on a regular basis in order to keep a standard quality and uniformed criteria. Desirably, active fault databases should indicate somehow the quality of the geological data and, particularly, the reliability attributed to crucial fault-seismic 15 parameters, as Maximum Magnitude and Recurrence Interval. In this paper we explain how we tackled these issues during the process of updating and reviewing the Quaternary Active Fault Database of Iberia (QAFI) to its current version 3. We devote particular attention to describing the scheme devised for classifying the quality and representativeness of the geological evidence of Quaternary activity and the accuracy of the slip rate estimation in the database. Subsequently, we use this information as input for a straightforward rating of the level of reliability of Maximum Magnitude and Recurrence Interval 20 fault seismic parameters. We conclude that QAFI v.3 is a much better database than version 2 either for a proper use in seismic hazard applications or as an informative source for non-specialized users. However, we already envision new improvements for a future update.
A case of Ehlers–Danlos syndrome presenting with widened atrophic scars of forehead, elbow, knee, and pretibial area

Abstract Rational: Ehlers–Danlos syndrome (EDS) is a heritable connective tissue disorder. Currently, the genotype-phenotype correlations of classical EDS (cEDS) are still controversial. Hence, this study reported a case of cEDS with both clinical manifestations and COL5A2 gene mutation. Patient concerns: A 30-year-old female presented to the plastic-surgery clinic with widen atrophic scars on forehead, elbows, knees and pretibial area that had developed since childhood. Diagnosis: With the skin hyperextensibility, joint hypermobility, papyraceous scar revealed by physical examination, and the heterozygous pathogenic variant c1997G > A (p.P659P) in COL5A2 gene revealed by whole exome sequencing, the diagnosis of the classical Ehlers–Danlos syndrome was made. Interventions: The patient underwent facial scar resection and sutured in minimizing tension and perfect apposition to avoid the post-surgery scar formation. Outcomes: Follow-up 6 months after surgery, the wound remained a fine line scar. Lessons: Our findings suggested that COL5A2 gene mutation (c1997G > A p.P659P) may be associated with cEDS but did not reveal other severe complications.
Echocardiography and Electrocardiography Variables Correlate With the New York Heart Association classification

Abstract The aim of our study was to determine whether combinations of ultrasound echocardiography (UCG) and electrocardiography (EKG) parameters correlated with the functional status of ischemic cardiomyopathy (ICM) patients according to the New York Heart Association (NYHA) classification system. We assessed 536 elderly Chinese ICM patients according to the NYHA criteria, which included 196 patients with type 2 diabetes mellitus (T2DM). All of the patients underwent UCG. Transmural dispersion of ventricular repolarization was examined using EKG. Cumulative odds logistic regression was performed to evaluate associations between NYHA class and the demographic, clinical, UCG, and EKG variables based on the odds ratio (OR) and 95% confidence interval (CI). A Pearson analysis was also performed to examine correlations between the NYHA classification and the UCG and EKG variables. Based on the NYHA assessment, 140, 147, 138, and 111 patients were identified as class I, II, III and IV, respectively. A comparison of UCG and EKG variables based on T2DM status showed that CO and Tp-e differed significantly between all NYHA classes (P < .05 for all), with values of each increasing with increasing NYHA class regardless of T2DM status. Multivariate logistic regression analysis showed that the disease course (OR: 1.30; 95% CI: 1.20–1.40), heart rate (OR: 1.16; 95% CI: 1.12–1.21), T wave peak to endpoint (Tp-e; OR: 1.22; 95% CI: 1.18–1.27), dispersion of the QT interval (OR: 0.98; 95% CI: 0.95–1.22), left ventricular fractional shortening (OR: 0.82; 95% CI: 0.78–0.87), cardiac output (CO; OR: 5.58; 95% CI: 3.08–10.13) were significantly associated with the NYHA class (P < .0001 for all). A Pearson correlation analysis showed that Tp-e (r = 0.75982, P < .0001), CO (r = 0.56072, P < .0001), and stroke volume (r = −0.14839, P = .0006) significantly correlated with the NYHA class. An index consisting of Tp-e and CO will be useful for corroborating the results of the NYHA assessment of ICM patients.