Miracle-dz/UniFAR-FTUs huggingface dataset:
All Polarization Receiving Rectenna With Harmonic Rejection Property for Wireless Power Transmission

In this paper, a high conversion efficiency rectenna for wireless power transmission at 2.45 GHz is proposed. The rectenna is fabricated on a low-cost FR-4 substrate. The proposed design contains a dual circularly polarized patch antenna in which a high-order harmonic rejection property is embedded, and a pair of rectifying circuits without harmonic rejection filters. The dimension for the proposed rectenna design is 100×100×3.8 mm3. The proposed rectenna collects waves in all polarization senses simultaneously with a suitable air gap width, chosen to maximize antenna gain, and provide high isolation between the rectifying circuits. The rectifying circuits are fed by the wave power of orthogonally polarized waves. The conversion efficiency is improved by blocking the second and the third harmonic noise from reemission without suffering loss from filters: the second harmonic noise is rejected with a T-shape slot, while the third is rejected by a U-shape resonator. The measured conversion efficiency of the proposed rectenna reaches 82.3% when the inputs of the rectifying circuits are fed at 22 dBm, surpassing the majority of existing rectenna designs in the current literature. The functionality of reception at all polarizations is also verified, distinguishing our design from other proposals.
Molecular Identification of the Ryanodine Receptor Ca2+ Sensor*

We have investigated the molecular basis for ryanodine receptor (RyR) activation by Ca2+ by using site-directed mutagenesis together with functional assays consisting of Ca2+ release measurements and single channel recordings in planar lipid bilayers. We report here that a single substitution of alanine for glutamate at position 3885 (located in the putative transmembrane sequence M2 of the type 3 RyR) reduces the Ca2+ sensitivity, as measured by single channel activation, by more than 10,000-fold, without apparent changes in channel conductance and in modulation by other ligands (e.g. ATP and ryanodine). Co-expression of the wild type and mutant RyR proteins results in the synthesis of single channels that have intermediate Ca2+ sensitivities. These results suggest that the glutamates at position 3885 of each monomer may act in a coordinated way to form the Ca2+ sensor in the tetrameric structure corresponding to RyR.
Towards a Configurative Publication Schema for Design Science Research

Design science research (DSR) has matured and gained acceptance as an appropriate information systems research method. Despite the increasing number of DSR publications there is still no common sense and no comprehensive guidance how to present DSR in scientific literature. Therefore, this paper investigates the potential of a configurative DSR publication schema by means of a reference model allowing the deduction of concrete publication schemas. These schemas provide more detailed advice depending on the particular research context, such as the intended artifact type, the evaluation method, or the knowledge contribution type. By identifying configuration parameters (through an investigation of 13 DSR meta-analysis papers) and common configurations (through a meta-analysis of 52 DSR journal publications) we lay the foundations for a configurative reference model which can be adapted to provide detailed guidance in concrete DSR publication situations for both authors and reviewers. A detailed example sketches the future artifact.
Analysis of the relationship between single nucleotide polymorphism of the CD209, IL-10, IL-28 and CCR5 D32 genes with the human predisposition to developing tick-borne encephalitis.

Introduction: It is known that in the pathogenesis of tick-borne encephalitis (TBE) various molecules play a significant role. The most prominent factors include IL-10, IL-28B, CD-209 and CCR5. It is reasonable to search for genetic predispositions to the development of various clinical forms of TBE related to the genetic variation of IL-10, IL-28B, CD-209 and CCR5. In this study we aimed to search for the relationship between single nucleotide polymorphism in the promoter region of the CD209, IL-10, IL-28 and 32 base pair deletion in CCR5 coding region (Δ 32) with the human predisposition to development of various clinical presentations of TBE. We tried to assess the relation between the presence of particular alleles and genotypes with laboratory and clinical parameters. Material/Methods 59 patients with TBE and 57 people, bitten by a tick who never developed TBE (Polish cohort), were included in the study. To assess the distribution of single nucleotide polymorphisms, TaqMan SNP genotyping assays were used for IL10: rs1800872 and rs1800896, for CD 209 rs4804803 and rs2287886, rs12979860 for IL 28B SNPs according to the manufacturer's protocol using real-time PCR technology on the StepOne thermal cycler. Results Comparison between TBE patients and CG showed that in SNP rs2287886 CD 209 AG heterozygotes were more frequent in the TBE group, while homozygotes GG were more frequent in the CG group. Conclusions SNP rs2287886 CD 209 AG heterozygotes predispose humans to develop TBE. Single nucleotide polymorphism in the promoter region of the CD209, IL-10, IL-28 and CCR5 D32 genes does not correlate with the severity of TBE.
Spirometric screening: does it work?

Role of spirometric testing in smoking cessation Pulmonary function testing offers an easy, inexpensive, and non-invasive means of diagnosing and staging chronic lung disease.1,2 It provides information on both the presence of obstructive lung disease and restrictive lung disease and can provide insights on how patients might respond to treatment.3,4 Spirometric testing also provides prognostic information, with lung function measures predicting mortality and the development of lung cancer.5,6 Despite the valuable information that spirometric testing provides, it is underused in medical practices in much of the world. There are several reasons for this, including (1) problems with doing the procedure,7 (2) problems related to compensation, and (3) …
Development of new ricin A‐chain immunotoxins with potent anti‐tumor effects against human hodgkin cells in vitro and disseminated Hodgkin tumors in scid mice using high‐affinity monoclonal antibodies directed against the CD30 antigen

The lymphocyte activation marker CD30 has been shown to be an excellent target for the immunotherapy of human Hodgkin's lymphoma. In order to develop new potent immunotoxins (ITs) against CD30, we chemically linked 6 recently described monoclonal antibodies (MAbs) via SMPT to deglycosylated ricin A‐chain (dgA). Cross‐blocking experiments demonstrated that these MAbs, termed Ki‐2 to Ki‐7, recognize 3 different clusters on the CD30 antigen: Ki‐2, Ki‐4, Ki‐5 and Ki‐7 recognize cluster A; Ki‐6 recognizes cluster B; Ki‐3 binds to cluster C. Staining of 29 sections of normal human organs revealed no major cross‐reactivity of any MAbs tested. Binding to the CD30 antigen on LS40Cy Hodgkin cells was assessed by flow cytometry, and demonstrated high affinities for Ki‐2, Ki‐3 and Ki‐4. The concentration giving 50% of the mean fluorescence intensity (MFI50) was 0.58 μg/ml to 0.78 μg/ml. MAbs Ki‐5, Ki‐6, and Ki‐7 bound much more weakly. The staining intensity of the MAbs correlated with the cytotoxicity of the corresponding ITs. Ki‐2.dgA, Ki‐3.dgA and Ki‐4.dgA inhibited the protein synthesis of L540Cy cells by 50% at concentrations (IC50) of 3.5 × 10−l0 M to 4.0 × 10−11 M. The most effective IT, Ki‐4.dgA, is 5‐fold more potent than previously reported CD30 ricin A‐chain ITs. Ki‐4.dgA was subsequently used for the treatment of disseminated human Hodgkin's lymphoma in a SCID mouse model. The mean survival time (MST) of lymphoma‐bearing SCID mice was extended from 42 days in untreated controls to more than 132 days when Ki‐4.dgA was applied one day after tumor challenge. Ki‐4.dgA is a new potent IT suitable for further evaluation against Hodgkin's lymphoma in man.
Regional Transport and Urban Contributions to Fine Particle Concentrations in Southeastern Canada

Abstract The impact of various atmospheric transport directions on ambient fine particle (PM2.5) concentrations at several sites in southeastern Canada was estimated (for May-September) using back-trajectory analysis. Three-day back trajectories (four per day) were paired with 6-hr average PM2.5 mass concentrations measured using tapered element oscillating microbalances (TEOM). PM2.5 concentrations at rural locations in the region were affected by nonlocal sources originating in both Canada and the United States. Comparison of sites revealed that, on average, the local contribution to total PM2.5 in the greater Toronto area (GTA) is approximately 30–35%. At each location, average PM2.5 concentrations under south/southwesterly flow conditions were 2–4 times higher than under the corresponding northerly flow conditions. The chemical composition of both urban and rural PM2.5 was determined during two separate 2-week spring/summer measurement campaigns. Components identified included SO4 2−, NO3 −, NH4+, black carbon and organic carbon (OC), and trace elements. Higher particle mass at the urban Toronto site was composed of a higher proportion of all components. However, black carbon, NO3 −, NaCl, and trace elements were found to be the most enriched over the rural/regional background levels.
Dynamic Visualization of Signaling Activities in Living Cells

Engineered fluorescent reporters allow researchers to follow subcellular activities of signaling components in real time in live cells. The complexity and specificity of many forms of signal transduction are widely suspected to require spatial microcompartmentation and dynamic modulation of the activities of protein kinases, phosphatases, and second messengers. However, traditional methodologies for detecting signaling events, such as activation of kinases and second-messenger production and degradation, are limited in their spatiotemporal resolution and do not allow one to follow these events within the live-cell context. To achieve dynamic tracking of signaling activities in living cells, we have engineered genetically encoded fluorescent reporters for protein kinases and second messengers, such as cyclic adenosine monophosphate (cAMP) and phosphoinositides. Their development and specific examples of their application are discussed. In addition, a live-cell, high-throughput screening method has been developed for identification of new modulators that affect the dynamic activity of kinases and second messengers. Together, these reporters have the potential to provide important spatiotemporal information about the circuitry governing specific signaling events in living cells.
Ptc 1 p regulates cortical ER inheritance via Slt 2 p

Studies in the yeast Saccharomyces cerevisiae have shown that the inheritance of endoplasmic reticulum (ER), mitochondria, and vacuoles involves the capture of a tubular structure at the bud tip. Ptc1p, a serine/threonine phosphatase, has previously been shown to regulate mitochondrial inheritance by an unknown mechanism. Ptc1p regulates the high osmolarity glycerol mitogenactivated protein kinase (MAPK) pathway and has also been implicated in the cell wall integrity (CWI) MAPK pathway. Here we show that the loss of Ptc1p or the Ptc1p binding protein, Nbp2p, causes a prominent delay in the delivery of ER tubules to the periphery of daughter cells and results in a dramatic increase in the level of phosphorylated Slt2p, the MAPK in the CWI pathway. Either loss of Slt2p or inhibition of the CWI pathway by addition of sorbitol, suppresses the ER inheritance defect in the ptc1D and nbp2Dmutants. Our findings indicate that Ptc1p and Nbp2p regulate ER inheritance through the CWI MAPK pathway by modulating the MAPK, Slt2p. The EMBO Journal (2006) 25, 4413–4422. doi:10.1038/ sj.emboj. 7601319; Published online 14 September 2006 Subject Categories: membranes & transport
Polymorphisms in SLCO1B3 and NR1I2 as genetic determinants of hematotoxicity of carboplatin and paclitaxel combination.

AIM The goal of our study was to assess the impact of patients' genetic background on their sensitivity to carboplatin/paclitaxel hematotoxicity. PATIENTS & METHODS Parameters describing sensitivity to neutropenia and to thrombocytopenia of 201 patients were extracted from a previous pharmacokinetic/pharmacodynamics analysis, in order to assess their association with 52 candidates SNPs in 18 genes. RESULTS Carriers of a T allele of SLCO1B3-rs4149117 were 19% less sensitive to thrombocytopenia than the homozygotes for the G allele (p = 0.00279). Carriers of two copies of the ATG haplotypes of NR1I2-rs1523130, rs3814055 and rs1523127 were 19% less sensitive to thrombocytopenia than those harboring other haplotypes (p = 0.025). CONCLUSION Our results revealed the importance of SLCO1B3 and NR1I2 in the sensitivity to carboplatin/paclitaxel thrombocytopenia.
The Design and Validation of an Intuitive Confidence Measure

Explainable AI becomes increasingly important as the use of intelligent systems becomes more widespread in high-risk domains. In these domains it is important that the user knows to which degree the system’s decisions can be trusted. To facilitate this, we present the Intuitive Confidence Measure (ICM): A lazy learning meta-model that can predict how likely a given decision is correct. ICM is intended to be easy to understand which we validated in an experiment. We compared ICM with two different methods of computing confidence measures: The numerical output of the model and an actively learned metamodel. The validation was performed using a smart assistant for maritime professionals. Results show that ICM is easier to understand but that each user is unique in its desires for explanations. This user studies with domain experts shows what users need in their explanations and that personalization is crucial. © 2018
Cellular Internalization of Green Fluorescent Protein Fused with Herpes Simplex Virus Protein VP22 via a Lipid Raft-mediated Endocytic Pathway Independent of Caveolae and Rho Family GTPases but Dependent on Dynamin and Arf6*

VP22 is a structural protein of the herpes simplex virus and has been reported to possess unusual trafficking properties. Here we examined the mechanism of cellular uptake of VP22 using a fusion protein between the C-terminal half of VP22 and green fluorescent protein (GFP). Adsorption of VP22-GFP onto a cell surface required heparan sulfate proteoglycans and basic amino acids, in particular, Arg-164 of VP22. Inhibitor treatment, RNA interference, expression of dominant-negative mutant genes, and confocal microscopy all indicated that VP22-GFP enters cells through an endocytic pathway independent of clathrin and caveolae but dependent on dynamin and Arf6 activity. As with CD59 (a lipid raft marker), cell-surface VP22-GFP signals were resistant to Triton X-100 treatment but only partially overlapped cell-surface CD59 signals. Furthermore, unlike other lipid raft-mediated endocytic pathways, no Rho family GTPase was required for VP22-GFP internalization. Internalized VP22 initially entered early endosomes and then moved to lysosomes and possibly recycling endosomes.
CARCINOGEN-METABOLIZING ENZYME ACTIVITY IN THE LIVER : BENEFICIAL EFFECT OF THE PHYTOTHERAPEUTIC COMPOUND YHK 8 th World Congress on Gastrointestinal Cancer Barcelona , Spain . 28 th June ~ 1 st July 2006

In this study we investigated the effect of YHK supplementation on the activities of antioxidant, phase I and phase II metabolizing enzymes involved in detoxification as well on liver antioxidant defense system in rats. YHK was administered for four weeks to Wister rats. At the end of the treatment period, different cytochrome P450 (CYP) isoform and phase II enzyme activities were determined by incubation of the liver microsomes or cytosols with appropriate substrates Dietary supplementation of YHK (2%, w/v) to male rats for four weeks significantly increased the activities of glutathione peroxidase and catalase to 118% and 87% in liver as compared with corresponding normal diet fed control (P<0.05-0.001). CYP 1A2 activity was markedly increased in all the YHK treatment groups (P! 0.05). CYP 1A1 activity was increased significantly in all the groups.Parallel to these changes, YHK feeding to mice also resulted in a considerable enhancement in the activity of phase I and II metabolizing enzymes such as glutathione S-transferase to 1.6 fold and 1.8 fold in liver as compared with corresponding normal diet fed control (P<0.05-0.01). The induction of such detoxifying enzymes by YHK suggest the potential value of this compound as protective agent against chemical carcinogensis and other forms of electrophilic toxicity. The significance of these results can be implicated in relation to cancer chemopreventive effects of YHK against the induction of tumors in various target organs.
Effects of short-term physical training on the liver IGF-I in diabetic rats

To investigate the influence of short-term physical training on IGF-I concentrations in diabetic rats, male wistar rats were distributed into four groups: sedentary control, trained control, sedentary diabetic and trained diabetic. Diabetes was induced by Alloxan (32 mg/kg b.w.) and training protocol consisted of swimming 1 h/day, 5 days/week, during 4 weeks, supporting 5% b.w. At the end of this period, rats were sacrificed and blood was collected for determinations of serum glucose, insulin, albumin, IGF-I and hematocrit. Liver samples were used to determine glycogen, protein, DNA and IGF-I concentrations. Diabetes reduced insulin and IGF-I concentrations in blood and liver protein, ratio protein/DNA and IGF-I concentrations in liver and increased glycemia. Physical training reduced serum glucose and recovered hepatic glycogen stores in diabetic rats and reduced serum and liver IGF-I concentrations. In conclusion, short-term physical training improved the metabolic conditions of diabetic rats, despite of impairing liver and blood IGF-I concentrations.
Hyperion: A Wearable Augmented Reality System for Text Extraction and Manipulation in the Air

We develop Hyperion a Wearable Augmented Reality (WAR) system based on Google Glass to access text information in the ambient environment. Hyperion is able to retrieve text content from users' current view and deliver the content to them in different ways according to their context. We design four work modalities for different situations that mobile users encounter in their daily activities. In addition, user interaction interfaces are provided to adapt to different application scenarios. Although Google Glass may be constrained by its poor computational capabilities and its limited battery capacity, we utilize code-level offloading to companion mobile devices to improve the runtime performance and the sustainability of WAR applications. System experiments show that Hyperion improves users ability to be aware of text information around them. Our prototype indicates promising potential of converging WAR technology and wearable devices such as Google Glass to improve people's daily activities.
The sheathed flagellum of Brucella melitensis is involved in persistence in a murine model of infection

Persistence infection is the keystone of the ruminant and human diseases called brucellosis and Malta fever, respectively, and is linked to the intracellular tropism of Brucella spp. While described as non‐motile, Brucella spp. have all the genes except the chemotactic system, necessary to assemble a functional flagellum. We undertook to determine whether these genes are expressed and are playing a role in some step of the disease process. We demonstrated that in the early log phase of a growth curve in 2YT nutrient broth, Brucella melitensis expresses genes corresponding to the basal (MS ring) and the distal (hook and filament) parts of the flagellar apparatus. Under these conditions, a polar and sheathed flagellar structure is visible by transmission electron microscopy (TEM). We evaluated the effect of mutations in flagellar genes of B. melitensis encoding various parts of the structure, MS ring, P ring, motor protein, secretion apparatus, hook and filament. None of these mutants gave a discernible phenotype as compared with the wild‐type strain in cellular models of infection. In contrast, all these mutants were unable to establish a chronic infection in mice infected via the intraperitoneal route, raising the question of the biological role(s) of this flagellar appendage.
Sperm analysis by flow cytometry using the fluorescent thiol labeling agent monobromobimane

The fluorescent labeling agent monobromobimane (mBBr) was used to label thiols and disulfides (after reduction of sperm disulfides by dithiothreitol) in intact spermatozoa. Bimane‐labeled sperm of several mammalian species were analyzed by flow cytometry (FCM) and examined by fluorescent microscopy. FCM analysis showed sperm thiol oxidation to disulfides during epididymal maturation. FCM of labeled mature spermatozoa showed differences among species in the sperm thiol content. Heterogeneity in thiol content of sperm within individual samples was also observed. In addition, FCM patterns showed heterogeneity among and within samples in the content of disulfides and their resistance to reduction. FCM analysis reflected the microscopic appearance of the labeled spermatozoa. FCM analysis of bimanelabeled spermatozoa offers a convenient method for the study of sperm thiol–disulfide status and permits detection of sperm subpopulations within an individual sample. FCM analysis of mBBr‐labeled spermatozoa may serve as a test to evaluate sperm quality.
C3a and adult respiratory distress syndrome after massive transfusion

We compared the degree of complement activation in 15 patients receiving massive blood transfusions and 21 patients with the septic syndrome and examined its association with other risk factors for adult respiratory distress syndrome (ARDS). Patients with massive transfusion (n = 8) as their only risk factor for ARDS had lower C3a levels (250 ± 80 ng/ml) and a lower incidence of ARDS (0%) than patients with massive transfusion plus other risk factors (n = 7; C3a, 600 ± 120 ng/ml; ARDS, 55%) or patients with the septic syndrome (n = 21; C3a, 540 ± 80 ng/ml; ARDS, 19%). In our patients, the degree of complement activation appeared to reflect the presence of complicating clinical conditions.
Optimal bit allocation for video coding under multiple constraints

We consider optimal encoding of video sequences (for ATM networks) under buffer and channel constraints. For independent coding, we develop an algorithm which employs multiple Lagrange multipliers to find the constrained optimal bit allocation, Simulation results are presented. We also compare the coded video quality and other characteristics of variable bit-rate and constant bit-rate transmission.