Miracle-dz/UniFAR-FTUs huggingface dataset:
Rôle de ZNF217, un nouvel oncogène dans le cancer du sein : rôle dans l’échappement tumoral et valeur pronostique

ZNF217, un nouveau membre de la famille kruppel-like, est un facteur de transcription qui interagit avec des co-represseurs et des proteines modifiant les histones suggerant que ZNF217 ferait partie d’un complexe represseur de la transcription. ZNF217 serait un oncogene mais ses fonctions sont encore mal connues a l’heure actuelle. Les objectifs de ce travail ont ete d’etudier le role de ZNF217 dans l’echappement tumoral et sa valeur pronostique dans le cancer du sein. Ainsi, nous avons montre que de forts niveaux d’expression de ZNF217 sont associes a : (i) une augmentation de la proliferation cellulaire in vitro et de la croissance tumorale in vivo, (ii) la stimulation de l’invasion et de la migration cellulaire, (iii) l’induction de l’EMT via la voie du TGF-β. De plus, les cellules surexprimant ZNF217 sont resistantes au paclitaxel et cette resistance est associee a la deregulation de l’expression des membres de la famille Bcl-2 et d’Aurora-A. Enfin, nous avons montre pour la premiere fois que des forts niveaux d’expression d’ARNm de ZNF217 representent un nouveau marqueur de mauvais pronostic dans le cancer du sein et sont associes au developpement de metastases. ZNF217 semble donc jouer un role important dans la cancerogenese mammaire et des strategies therapeutiques ciblant directement ZNF217 ou ciblant ses mediateurs (Aurora-A ou TGF-β) pourraient etre utilisees en clinique dans le traitement des tumeurs mammaires surexprimant ZNF217
National identities : the constitution of the United Kingdom

1. Four Nations or One? : Hugh Kearney (University of Pittsburgh) 2. The British and the Irish in the Context of Europe: Garret Fitzgerald TD, former Prime Minister of Ireland. 3. Nations, Regions and Europe: David Marquand (University of Sheffield). 4. Scottish Identity and the Constitution: Robert McCreadie (University of Edinburgh). 5. The Constitution of Wales: Dafydd Ellis Thomas MP (Plaid Cymru Member of Parliament). 6. Northern Ireland: Allegiances and Identities: Kevin Boyle (University of Essex). 7. Ireland: Identity and Relationships: John A. Murphy (University College, Cork). 8. The English and British: Bernard Crick (Birkbeck College, London, and University of Edinburgh). 9. English Regionalism: the Dog that Never Barked: Christopher Harvie (University of Tubingen). 10. Autonomy in Spain: Lessons for Britain? : Tom Gallagher (University of Bradford). 11. Europe and the Regions: David Coombes (University of Limerick). 12. The Road to Constitution: J. M. Ross (former civil servant, Scottish Office). 13. Towards a Constitutional Equation? : James Cornford (Institute for Public Policy Research). 14. Appendix: Two Editorials: The Guardian .
Life-Threatening Food Refusal in Two Nine-Year-Old Girls: Re-Thinking the Maudsley Model

While there is growing evidence for the efficacy of the Maudsley model of family-based treatment for anorexia nervosa, little is known concerning the most effective treatment for pre-adolescents with Eating Disorders Not Otherwise Specified (EDNOS). The presentation of case studies of children with EDNOS is important. Large-scale studies are difficult due to low incidence and the application of homogenous manualized approaches may be unsuitable to the wide range of potential disorders. The aim of this article is to present two detailed case studies of children with food avoidant emotional disorder, both presenting with life threatening food refusal. A flexible and individualized approach to family therapy was taken, resulting in the resumption of eating in less than seven weeks and four months respectively.
Antioxidants for male subfertility.

BACKGROUND Between 30% to 80% of male subfertility cases are considered to be due to the damaging effects of oxidative stress on sperm. Oral supplementation with antioxidants may improve sperm quality by reducing oxidative stress. OBJECTIVES This Cochrane review aimed to evaluate the effect of oral supplementation with antioxidants for male partners of couples undergoing assisted reproduction techniques (ART). SEARCH STRATEGY We searched the Cochrane Menstrual Disorders and Subfertility Group Register, CENTRAL (The Cochrane Library), MEDLINE, EMBASE, CINAHL, PsycINFO and AMED databases (from their inception until Febuary 2010), trial registers, sources of unpublished literature, reference lists and we asked experts in the field. SELECTION CRITERIA We included randomised controlled trials comparing any type or dose of antioxidant supplement (single or combined) taken by the male partner of a couple seeking fertility assistance with placebo, no treatment or another antioxidant. The outcomes were live birth, pregnancy, miscarriage, stillbirth, sperm DNA damage, sperm motility, sperm concentration and adverse effects. DATA COLLECTION AND ANALYSIS Two review authors independently assessed studies for inclusion and trial quality, and extracted data. MAIN RESULTS We included 34 trials with 2876 couples in total. Live birth: three trials reported live birth. Men taking oral antioxidants had an associated statistically significant increase in live birth rate (pooled odds ratio (OR) 4.85, 95% CI 1.92 to 12.24; P = 0.0008, I(2) = 0%) when compared with the men taking the control. This result was based on 20 live births from a total of 214 couples in only three studies.Pregnancy rate: there were 96 pregnancies in 15 trials including 964 couples. Antioxidant use was associated with a statistically significant increased pregnancy rate compared to control (pooled OR 4.18, 95% CI 2.65 to 6.59; P < 0.00001, I(2) = 0%). Side effects: no studies reported evidence of harmful side effects of the antioxidant therapy used. AUTHORS' CONCLUSIONS The evidence suggests that antioxidant supplementation in subfertile males may improve the outcomes of live birth and pregnancy rate for subfertile couples undergoing ART cycles. Further head to head comparisons are necessary to identify the superiority of one antioxidant over another.
Effects of postexercise feeding of a supplemental carbohydrate and protein bar with or without astaxanthin from Haematococcus pluvialis to exercise-conditioned dogs.

OBJECTIVE To characterize the postprandial nutrient profiles of exercise-conditioned dogs fed a supplemental carbohydrate and protein bar with or without astaxanthin from Haematococcus pluvialis immediately after exercise. ANIMALS 34 exercise-conditioned adult Husky-Pointer dogs. PROCEDURES The study had 2 phases. During phase 1, postprandial plasma glucose concentration was determined for dogs fed a bar containing 25% protein and 18.5% or 37.4% maltodextrin plus dextrin (rapidly digestible carbohydrate; RDC), or dry kibble (30% protein and 0% RDC) immediately after exercise. During phase 2, dogs were exercised for 3 days and fed a bar (25% protein and 37.4% RDC) with (CPA; n = 8) or without (CP; 8) astaxanthin or no bar (control; 8) immediately after exercise. Pre- and postexercise concentrations of plasma biochemical analytes and serum amino acids were determined on days 1 and 3. RESULTS Phase 1 postexercise glucose concentration was increased when dogs were provided the 37.4% RDC bar, but not 0% or 18.5% RDC. On day 3 of phase 2, the CPA group had the highest pre-exercise triglyceride concentration and significantly less decline in postexercise glucose concentration than did the CP and control groups. Mean glucose concentration for the CP and CPA groups was significantly higher than that for the control group between 15 and 60 minutes after bar consumption. Compared to immediately after exercise, branched-chain amino acid, tryptophan, leucine, and threonine concentrations 15 minutes after exercise were significantly higher for the CP and CPA groups, but were lower for the control group. CONCLUSIONS AND CLINICAL RELEVANCE Dogs fed a bar with 37.4% RDCs and 25% protein immediately after exercise had increased blood nutrient concentrations for glycogen and protein synthesis, compared with control dogs.
The Study of Composite Right/Left Handed Structure in Substrate Integrated Waveguide

The former transmission lines for composite right/left-handed (CRLH) structures are based on microstrip line or co-planar waveguides (CPW). In this paper, new CRLH structures are proposed based on substrate integrated waveguide (SIW) with high Q-factors and low radiations. Two models are presented and one of them is carefully analyzed with the support of simulation results.
A Distinct Subset of Atypical Spitz Tumors is Characterized by BRAF Mutation and Loss of BAP1 Expression

We recently reported that germline mutations in BAP1 cause a familial tumor syndrome characterized by high penetrance for melanocytic tumors with distinct clinical and histologic features. Melanocytic neoplasms in affected individuals harbored BRAF mutations, showed loss of BAP1 expression, and histologically resembled so-called “atypical Spitz tumors” (ASTs). ASTs are an ill-defined and probably heterogenous group of melanocytic tumors that display histologic features seen in both Spitz nevi and melanomas. Their biological behavior cannot be reliably predicted. In view of the histologic similarities of the familial tumors and ASTs, we hypothesized that a subset of ASTs might harbor genetic alterations seen in the familial tumors. To address this hypothesis, we analyzed 32 sporadic ASTs for BRAF mutations and for BAP1 expression. Nine (28%) sporadic ASTs showed loss of BAP1 expression, of which 8 (89%) had concomitant BRAF mutations. Only 1 of the BAP1-positive ASTs (4%) had a BRAF mutation (P<0.0001). BRAF-mutated, BAP1-negative tumors were primarily located in the dermis and were composed entirely or predominantly of epithelioid melanocytes with abundant amphophilic cytoplasm and well-defined cytoplasmic borders. Nuclei were commonly vesicular and exhibited substantial pleomorphism and conspicuous nucleoli. The combination of BRAF mutation and loss of nuclear BAP1 expression thus characterizes a subset of ASTs with distinct histologic features. The typical morphology of these tumors and BAP1 immunohistochemistry provide pathologic clues that will enable accurate identification of this subset. Future studies are necessary to determine whether this subset has a predictable clinical behavior.
Robotic facelift thyroidectomy: I. Preclinical simulation and morphometric assessment

Robotic thyroidectomy was introduced in the United States despite scant preclinical data. We pursued a systematic preclinical investigation of a new remote access, robotic thyroidectomy technique via a facelift incision, and sought to define differences in extent of dissection associated with this approach and a second, popular robotic thyroidectomy technique.
Angiotensin peptides and nitric oxide in cardiovascular disease.

SIGNIFICANCE The renin-angiotensin system (RAS) plays an important role in the normal control of cardiovascular and renal function in the healthy state and is a contributing factor in the development and progression of various types of cardiovascular diseases (CVD), including hypertension, diabetes, and heart failure. RECENT ADVANCES Evidence suggests that a balance between activation of the ACE/Ang II/AT1 receptor axis and the ACE2/Ang-(1-7)/Mas receptor axis is important for the function of the heart, kidney, and autonomic nervous system control of the circulation in the normal healthy state. An imbalance in these opposing pathways toward the ACE/Ang II/AT1 receptor axis is associated with CVD. The key component of this imbalance with respect to neural control of the circulation is the negative interaction between oxidative and NO• mechanisms, which leads to enhanced sympathetic tone and activation in disease conditions such as hypertension and heart failure. CRITICAL ISSUES The key mechanisms that disrupt normal regulation of Ang II and Ang-(1-7) signaling and promote pathogenesis of CVD at all organ levels remain poorly understood. The reciprocal relation between ACE and ACE2 expression and function suggests they are controlled interdependently at pre- and post-translational levels. Insights from neural studies suggest that an interaction between oxidative and nitrosative pathways may be key. FUTURE DIRECTIONS The role of redox mechanisms in the control of expression and activity of RAS enzymes and Ang receptors may provide important insight into the function of local tissue RAS in health and disease.
EXPRESSION OF HYPOXIA‐INDUCING FACTOR‐1α AND ENDOGLIN IN INTIMAL HYPERPLASIA OF THE MIDDLE CEREBRAL ARTERY OF PATIENTS WITH MOYAMOYA DISEASE

OBJECTIVEMoyamoya disease (MMD) is a cerebrovascular occlusive disease characterized by progressive stenosis or occlusion at the distal ends of the bilateral internal arteries. In MMD, intimal hyperplasia was previously reported to be found in autopsy samples. In this study focusing on the mechanism of remodeling of the intracranial arterial walls of patients with MMD, we surgically collected tiny pieces of the wall of the middle cerebral artery (MCA) from patients with MMD and analyzed them using histological and immunohistochemical methods. METHODSTwelve patients underwent surgical procedures for treatment of standard indications of MMD at Kyoto University Hospital. Specimens of MCA were obtained from MMD patients during the surgical procedures. Nine MCA samples were also obtained in the same way from control patients. The samples were analyzed by immunohistochemical methods. RESULTSMCA specimens from MMD patients had a thicker intima than those from the control group. In MMD samples, the immunoreactivity indicating hypoxia-inducing factor-1α was higher in the endothelium and intima; endoglin expression was also higher in the endothelium. No vascular endothelial growth factor immunoreactivity was detectable in the MMD samples. In addition, transforming growth factor-β3 immunoreactivity was also detected and was co-localized with that of hypoxia-inducing factor-1α and endoglin, mainly in the endothelium. CONCLUSIONOur results indicate that the MCA specimens from MMD patients had thicker intimal walls than the specimens from control patients. In addition, hypoxia-inducing factor-1α and endoglin were overexpressed in the intima of the MCA of MMD patients.
Effects of hyperbaric oxygen therapy on the viability of irradiated soft head and neck tissues in mice.

OBJECTIVES Hyperbaric oxygen therapy (HBOT) is used clinically in irradiation-induced injury to healthy tissues, but the effectiveness and working mechanism remain unclear. This study examined the effects of HBOT on irradiated salivary glands and tongue in a mouse model. MATERIALS AND METHODS Mice were irradiated with a single dose (15 Gy) in the head and neck region and subjected to HBOT, either before or after irradiation. During the course of the treatments, salivary flow rates were measured and at different time points after radiation (2, 6, 10 and 24 weeks), salivary glands and tongue were harvested and (immuno) histochemically analysed. RESULTS Proliferation and blood vessel density in salivary glands were enhanced by HBOT in the medium term (10 weeks after irradiation), while salivary flow rates were not influenced. In the long term, irradiation-induced proliferation in the muscle tissue of the tongue was decreased by HBOT. CONCLUSION Hyperbaric oxygen therapy (HBOT) appears to stimulate regeneration or protection of salivary gland tissue following radiation therapy. Possible implications of the effect of HBOT on muscle tissue of the tongue for the prevention of dysphagia and trismus are discussed. This study provides insights on the cellular changes after HBOT and encourages further research on this topic to achieve a better implementation of the therapy in humans.
Caractérisation phonétique et phonologique du syntagme intermédiaire en français : de la production à la perception.

Le travail presente ici est sous-tendu par deux observations majeures. Premierement, la plupart des modeles proposes pour le francais s'accordent sur l'existence de deux niveaux de structure prosodique: le syntagme accentuel et le syntagme intonatif. Deuxiemement, bien que l'existence d'un niveau additionnel de structure situe entre ces deux niveaux ait ete propose pour le francais, les proprietes phonetiques et phonologiques de ce constituant n'ont pas clairement ete definies. Dans cette these nous avons fourni des preuves de l'existence du syntagme intermediaire (ip) a la fois en production et en perception de la parole. Grâce a cinq experimentations menees dans le cadre de la phonologie de laboratoire, nous avons caracterise les proprietes phonetico-phonologiques de ce constituant et atteste de son role dans le traitement perceptif du langage. Les resultats obtenus en production montrent que l'ip est le domaine de l'abaissement des accents melodiques en francais. Sa frontiere droite est marquee par un allongement pre-frontiere ainsi qu'un accent de syntagme responsable du retour a la ligne de reference du registre. Les analyses menees en perception ont montre que les frontieres droites du syntagme accentuel et du syntagme intermediaire sont utilisees tres tot dans le processus de traitement syntaxique. Les indices phonetiques et phonologiques presents a ces frontieres permettent aux auditeurs du francais de construire des attentes sur la structure syntaxique des enonces percus. Une analyse separee des differents types d'indices acoustiques a egalement montre qu'en l'absence de marquage tonal, les indices de duree semblent suffisants dans le but de marquer la frontiere de syntagme accentuel. Un marquage conjoint de la frontiere droite d'ip par les indices melodiques et l'allongement pre-frontiere semble au contraire necessaire pour que les auditeurs du francais percoivent et utilisent cette frontiere dans le traitement du langage.
A Critical Role for the TIFY Motif in Repression of Jasmonate Signaling by a Stabilized Splice Variant of the JASMONATE ZIM-Domain Protein JAZ10 in Arabidopsis[C][W]

JASMONATE ZIM-domain (JAZ) proteins act as repressors of jasmonate (JA) signaling. Perception of bioactive JAs by the F-box protein CORONATINE INSENSITIVE1 (COI1) causes degradation of JAZs via the ubiquitin-proteasome pathway, which in turn activates the expression of genes involved in plant growth, development, and defense. JAZ proteins contain two highly conserved sequence regions: the Jas domain that interacts with COI1 to destabilize the repressor and the ZIM domain of unknown function. Here, we show that the conserved TIFY motif (TIFF/YXG) within the ZIM domain mediates homo- and heteromeric interactions between most Arabidopsis thaliana JAZs. We have also identified an alternatively spliced form (JAZ10.4) of JAZ10 that lacks the Jas domain and, as a consequence, is highly resistant to JA-induced degradation. Strong JA-insensitive phenotypes conferred by overexpression of JAZ10.4 were suppressed by mutations in the TIFY motif that block JAZ10.4–JAZ interactions. We conclude that JAZ10.4 functions to attenuate signal output in the presence of JA and further suggest that the dominant-negative action of this splice variant involves protein–protein interaction through the ZIM/TIFY domain. The ability of JAZ10.4 to interact with MYC2 is consistent with a model in which a JAZ10.4-containing protein complex directly represses the activity of transcription factors that promote expression of JA response genes.
THERMOREGULATION UNDER CONDITIONS OF IMPAIRED BODY FLUID/OSMOTIC BALANCE IN MAMMALS

The state of body hydration has a marked effect on thermoregulation during heat stress. Heat stressed hypohydrated mammals reduce evaporative cooling and metabolic rate, and allow their body temperature to rise. This outcome is not a failure in thermoregulation, but readjustment, leading to improved physical avenues for heat dissipation and water conservation. The modulation of the thermoregulatory system is mostly central. This is exhibited by decreased sensitivity of the thermoregulatory integration center and by an upward shift of temperature thresholds for heat dissipation activities. New evidence suggests alterations in effector organs as well. In the euhydrated body, hyperosmolarity and hypovolemia, the consequences of hypohydration, via activation of osmoreceptors and low pressure baroreceptors, induce the same thermoregulatory responses as hypohydration does. Single unit studies on hypothalamic slices provide a neuronal basis for osmoregulation-thermoregulation interaction. The possible role of arginine vasopressin (AVP) as a central modulator in this interaction is discussed.
Fulfilling Ethical Responsibility: Moving Beyond the Minimal Standards of Protecting Human Subjects from Research Harm

Problem: Rules for protecting human subjects, in place federally since 1974, have focused primarily on guarding against placing research subjects at social, physical, or psychological risk or violating their privacy and confidentiality. Nevertheless, high-risk communities are routinely subjected to “sins of omission,” which limit access to potentially significant research opportunities and result in the absence of studies that could confer high degree of community beneficence.Purpose of Article: To describe “sins of omission” and provide examples from the Community Networks Program Centers (CNPC) to illustrate how community-based participatory research (CBPR) can prevent them.Key Points: CBPR is an effective antidote to sins of omission. Activities undertaken by the CNPCs illustrate how adherence to CBPR principles can improve research access and outcomes.Conclusions: By working with community members as partners, we expand the concept of beneficence to include “community beneficence,” thus reducing the probability of “sins of omission. ”
Cost analysis of surgical site infections.

BACKGROUND Patients with surgical site infections (SSIs) require a longer time in the hospital, more nursing care, additional dressings, and, possibly, readmission to the hospital and further surgery. The combined direct and indirect costs of treating SSIs may be extremely high. METHODS Review of current practice and guidelines. RESULTS The direct costs of SSI include a longer hospital stay, readmission, outpatient and emergency visits, further surgery, and prolonged antibiotic treatment. Other direct costs arise from radiologic procedures, laboratory tests, home health visits and other ancillary services, drugs, and professional fees. Indirect costs, which are difficult to quantify, include lost productivity of the patient and family and a temporary or permanent decline in functional or mental capacity. The cost of SSIs increases with the depth of the infection. That is, the costs associated with superficial incisional SSIs are relatively low, but increase with deep SSI, and especially when organ or space infection is present. The estimated costs of managing SSI differ widely, from less than dollar 400 per case for superficial SSI to more than dollar 30,000 per case for serious organ or space infections. CONCLUSIONS The need to treat SSIs places a severe financial strain on health care resources. It is possible that treating high-risk surgical patients medically will prove to be more cost-effective than repeated operations.