Miracle-dz/UniFAR-FTUs huggingface dataset:
Pengaruh Penyuntikan Prostaglandin terhadap Persentase Birahi dan Angka Kebuntingan Sapi Bali dan Po di Kalimantan Selatan

ABSTRAK Penelitian ini bertujuan untuk mengetahui respon birahi dan angka kebuntingan pada sapi Bali dan PO di Kalimantan Selatan melalui gertak birahi dengan prostaglandin (PGF2? Jumlah sapi yang digunakan sebanyak 69 ekor yang terdiri atas 23 ekor sapi Bali dan 46 ekor sapi Peranakan Ongole (PO) yang tersebar di tiga desa. Semua sapi disuntik dengan PGF2? ebanyak dua kali dengan jarak penyuntikan 11 hari. Tiga hari setelah penyuntikan PGF2? ang kedua, dilakukan inseminasi buatan dan setelah tiga bulan dilakukan pengamatan terhadap angka kebuntingan. Data mengenai persentase estrus dan angka kebuntingan dianalisis dengan menggunakan uji ? Chi-Square? Hasil penelitian menunjukkan bahwa setelah penyuntikan pertama dengan PGF2? persentase birahi sapi PO (67,39%) lebih tinggi secara sangat nyata daripada sapi Bali (39,13%), dari semua sapi birahi setelah penyuntikan kedua. Angka kebuntingan sapi Bali (83,33%) lebih tinggi secara sangat nyata daripada sapi PO (47,37%).
La Clasificación de Sitios y el Crecimiento del Cupressus Lusitánica en Antioquia, Colombia.

En plantaciones jovenes de Cupressus lusitanica, en la Cordillera Central del Departamento de Antioquia, Colombia, se midieron 57 parcelas de las cuales se derivaron curvas de indice de sitio. Por medio de analisis de regresion de desarrollo una tabla de rendimiento expresando el volumen, area basal, y diametro a la altura del pecho en funcion de la edad y del indice de sitio. Fueron notables, la amplia variacion de la productividad entre sitios y la excesiva densidad de variacion de la productividad entre sitios y la excesiva densidad de las plantaciones. Dentro de distancias de pocos metros el indice de sitio (con edad base de 15 anos) puede variar entre 7 y 21 m. y el volumen en pie a los 15 anos puede fluctuar entre 30 y 300m3/ha. Para el Oriente Antioqueno se desarrollo un metodo sencillo para pronosticar el indice de sitio antes de plantar, con base en la pendiente y en una clasificacion visual de la convexidad o concavidad topografica. Esta especie parece excepcionalmente sensible al sitio y su crecimiento es lento sobre formas topograficas convexas.
Registro de Moridae (Teleostei: Gadiformes) en la Formación Chenque (Oligoceno Tardío-Mioceno Temprano) de la provincia de Chubut, Argentina

Record of Moridae (Teleostei: Gadiformes) from the Chenque Formation (Late Oligocene - Early Miocene) from the Chubut province, Argentina. A nearly complete specimen belonging to the family Moridae is described in this paper. The material has been collected in outcrops referable to the Chenque Formation (Oligocene-Miocene) from the Chubut province, Argentina. The specimen constitutes the first fossil record for the Gadiformes in Argentina and one of the first osteological occurrences for Moridae. The morphology of the specimen suggests affinities with the genera Physiculus and Salilota. The presence of Moridae in the Early Miocene of Patagonia and New Zealand is in agreement with previous hypotheses suggesting marine transantarctic connections between both landmasses during Late Oligocene - Early Miocene times.
Implication du systeme endomembranaire dans la replication du virus du court-noue de la vigne (gflv) : role de la proteine 2 a dans la replication du rna2

Le grapevine fanleaf virus (gflv), membre de la super-famille des picornaviridae est responsable de la maladie du court-noue de la vigne. Son genome est reparti sur deux rna. Le rna1 capable de se repliquer seul, code pour la polyproteine p1 auto-maturee par la proteinase 1dpro en proteines 1a, 1b, 1cvpg, 1dpro et 1epol, pour constituer le complexe de replication. Le rna2 depend du rna1 pour sa replication et code pour la polyproteine p2 maturee par 1dpro en proteines 2a de fonction inconnue, 2bmp et 2ccp necessaires a l'infection systemique des plantes. Des mutants de deletion ont montre que la replication du rna2 depend de la presence de la proteine 2a pour etudier son role et la localiser dans la cellule, la proteine 2a a ete fusionnee a la proteine fluorescente verte (gfp). Cette proteine de fusion (2agfp) permet la replication du rna2. Elle est localisee autour du noyau dans les cellules infectees, a l'endroit ou se trouvent les domaines 1cvpg, 1dpro, et ou est synthetise le rna viral trace par l'incorporation de brutp. La proteine 2a s'associe aux membranes du reticulum endoplasmique, tout comme les proteines du complexe de replication qui provoquent un recrutement de membranes dans la zone perinucleaire en detournant le trafic vesiculaire de maniere similaire au poliovirus. Le role de la proteine 2a est d'associer la polyproteine p2 en cours de traduction aux membranes du reticulum endoplasmique. Le complexe de traduction est alors recrute par le flux de membranes attire par le complexe de replication du rna1. De cette maniere le rna2 est pris en charge par le complexe de replication et la polyproteine p2 est maturee.
Wissensarbeit: Aktuelle arbeitswissenschaftliche Erkenntnisse

Digitalisierte Produktionsprozesse und neue Technologien verandern Arbeitsweisen und -ablaufe, Kommunikation mit Kollegen, Vorgesetzten und Kunden. Aus arbeitswissenschaftlicher Sicht entstehen neue Belastungen und Beanspruchungen fur Beschaftigte. Wissensarbeit ist fokussiert darauf, neues Wissen und Informationen zu erwerben und anzuwenden. Wissensarbeiter sind oft raumlich und zeitlich flexibel, arbeiten mobil. Folgen mobiler Arbeit und komplexe Wirkmechanismen sind nicht hinreichend untersucht. Daher wurde zunachst der aktuelle Stand arbeitswissenschaftlicher Erkenntnisse pragnant zusammengetragen. Offene Fragen: Wie lassen sich Ruhe- und Erholzeiten bei unterschiedlichen Belastungsbedingungen und personlichen Konstellationen definieren? Wie wirken sich selbstverantwortliches und selbst organisiertes Arbeiten aus? Inwieweit wird Arbeitszeit wirklich frei und selbstbestimmt festgelegt? Inwieweit erzeugen Zeitdruck, standige Erreichbarkeit und Selbstausbeutung eine verhaltnismasig grosere Belastung als bei weniger selbstbestimmten Arbeitsformen?
Working Outside the Protein-Synthesis Rules

Peptides manufactured by so-called nonribosomal peptide synthetases (NRPSs) include some of the most potent pharmaceuticals known, from penicillin to the immunosuppressant cyclosporin. Researchers hope to alter the NRPS machinery to make even more effective variants of these powerful drugs as well as novel drugs built along the same lines. Although the goal of designing drug-factory enzymes is still a long way off, recently researchers have begun to engineer new nonribosomal proteins.
Artificial leaf aids analysis of chlorophyll fluorescence and P700 absorbance in studies involving microalgae

Measuring chlorophyll fluorescence and P700 absorbance has been widely used to study photosynthesis in both terrestrial plants and algae. However, in order to apply these measurement techniques to study microalgae, a concentrated suspension of algae, which is usually prepared by centrifugation, is required. In this study, instead of using centrifugation, we concentrated microalgae on a nitrocellulose membrane using filtration to create an ‘artificial leaf’ before analysis. Overall, we were able to generate values of the appropriate photosynthetic parameters that were comparable to those obtained when chlorophyll fluorescence and P700 absorbance were measured following centrifugation. There were no statistically significant differences (P > 0.05) between the artificial leaf method and the traditional cuvette method for determining chlorophyll fluorescence or P700 absorbance at appropriate chlorophyll concentrations. We were also able to reduce background noise by using a filter membrane as a carrier. Therefore, an artificial leaf has the potential to be a valuable tool for phycologists interested in studying microalgal photosynthesis by enabling them to eliminate tedious centrifugation steps. In addition, fluorometers commonly used for studying the leaves of higher plants will also be suitable for studying microalgae.
On the fracture of solar grade crystalline silicon wafer

La rentabilite des cellules a base de silicium est un point essentiel pour le marche photovoltaique et cela passe notamment par l'amelioration du rendement electrique, la baisse des couts de production ainsi que le renforcement de la fiabilite/durabilite des wafers. Des procedes innovants emergent, qui permettent d'obtenir des wafers ultra minces avec moins de perte de matiere premiere. Cependant il est necessaire de mettre en place des methodes de caracterisation afin d’analyser la rigidite et la tenue mecanique de ces materiaux. Dans ce travail, des essais de flexion ont ete effectues pour caracteriser a la fois la rigidite et la rupture. Afin d’etudier la rupture fragile, une camera rapide a ete utilisee, des analyses fractographiques ont ete menees. La diffraction d'electrons retrodiffuses et la diffraction par rayon X de Laue ont ete utilisees afin d'explorer le lien entre les orientations cristallographiques et les comportements observes. Conjointement, des simulations numeriques EF ont ete mise en place. Grâce a ce couplage experiences-simulations numeriques, une caracterisation fiable de la rigidite des wafers a ete effectuee. Une strategie d'identification de l'origine de la rupture est egalement proposee. L'etude de la rupture du silicium monocristallin a mis en evidence la stabilite du clivage (110), la grande vitesse d'amorcage de la fissure, la dependance de la forme du front de fissure a la vitesse de propagation ainsi que l'apparition de "Front Waves" pour les fissures a tres grande vitesse. L'etude de la rupture des wafers multi-cristallins demontre une fissuration intra-granulaire. Des eprouvettes jumelles ont permis d’etudier la repetabilite du chemin de fissuration : une attention particuliere a ete portee a la nature des plans de clivage ainsi que l'effet des joints de grains. Enfin, une modelisation par la methode des elements finis etendus est proposee. Elle permet de reproduire le chemin de fissuration experimentalement observe.
Affordances in Interaction: The Case of Aikido

ABSTRACT Our title can be read as trivially true, namely, that perceived affordances shape real-time interaction dynamics. A less trivial reading suggests that affordances themselves interact in a shared dyadic field, such that the number and quality of As and Bs affordances are dynamically coupled with bidirectional causality. In dance, martial arts, or team sports agents strategically comodulate each other's affordances while pursuing their aims. In Aikido, where agents try to break their opponents' balance, this trade-off globally approximates a zero-sum game—the better A's affordances are, the lousier B's affordances get. The agents are subject to ceaseless cross-causation in this shared field. They seek to obstruct their opponents' options while strategically enabling, augmenting, and sculpting their own by employing subtle perceptual manipulation skills, redirecting force, brinkmanship, and switching techniques opportunistically. To overcome static views, we conceptualize affordances as cascading and having fluid onsets; we also identify nested affordances in goal hierarchies and describe a spectrum of affordance functions. Ultimately, we suggest rethinking the ontology of affordances as being sensitive to dynamic engagements, hence defined relative to interpersonal emergence.
The effects of drought and herbivory on plant–herbivore interactions across 16 soybean genotypes in a field experiment

As the Earth's climate continues to change, drought and insect population outbreaks are predicted to increase in many parts of the world. It is therefore important to understand how changes in such abiotic and biotic stressors might impact agroecosystems. The plant stress hypothesis predicts that, owing to physiological and biochemical changes, plants experiencing drought will be more susceptible to insect herbivory, which could have synergistic negative effects on plant performance. By contrast, the plant vigor hypothesis predicts that insects will preferentially feed on fast‐growing vigorous plants. These hypotheses were tested in a field experiment using 16 soybean (Glycine max (L.) Merr. ) genotypes to determine: (i) the combined effects of drought and herbivory on plant performance; (ii) the impact of drought on soybean resistance to herbivores; and (iii) how genetically variable phenotypic traits in soybean correlate with these responses. It was found that drought had a greater effect on soybean performance than herbivory, and drought and herbivory did not interact to impact on any measure of plant performance. Drought caused decreased insect herbivory on average, suggesting that the plant vigor hypothesis is consistent with the effects of drought stress on soybean resistance to leaf‐chewing insect herbivores. This conclusion is further supported by genotypic correlations which show that plant growth rate is positively correlated with the amount of herbivory plants received. These results suggest that, although the effects of climate‐associated changes in drought and herbivory will have negative effects on soybean, these potential effects are quantifiable with simple experiments and can be mitigated through continued breeding of varieties that are tolerant and resistant to these abiotic and biotic stressors.
GLUT1 Structure Function; Context, Ligand Cooperativity, and Mutagenesis Studies: A Dissertation

Carrier mediated nutrient import is vital for cell and tissue homeostasis. Structural insights of carrier mediated transport, particularly the human glucose transporter GLUT1, are essential for understanding the mechanisms of human metabolic disease, and provide model systems for cellular processes as a whole. GLUT1 function and expression is characterized by a complexity unexplained by the current hypotheses for carrier-mediated sugar transport (9) . It is possible that the operational properties of GLUT1 are determined by host cell environment. A glucose transport-null strain of Saccharomyces cerevisiae (RE700A) was transfected with the p426 GPD yeast expression vector containing DNA encoding the wild-type human glucose transport protein (GLUT1) to characterize its functional properties. Identical protein sequences generated different kinetic parameters when expressed in RE700A yeast, erythrocytes, and HEK293 cells. These findings support the hypothesis that red cell sugar transport complexity is host cell-specific. Cytochalasin B (CB) and forskolin (FSK) inhibit GLUT1-mediated sugar transport in red cells by binding at or close to the GLUT1 sugar export site. Paradoxically, very low concentrations of these inhibitors produce a modest stimulation of sugar transport (16) . This result is consistent with the hypothesis that the glucose transporter contains multiple, interacting, intracellular binding sites for e1 ligands CB and FSK. The present study tests this hypothesis directly and, by screening a library of cytochalasin and forskolin analogs, asks what structural features of exit site ligands determine binding site affinity and cooperativity. Our findings are explained by a carrier that presents at least two interacting endofacial binding sites for CB or FSK. We discuss this result within the context of GLUT1 quaternary structure and evaluate the major determinants of ligand binding affinity and cooperativity. Cytochalasin B (CB) inhibits GLUT1 substrate transport at or near the endofacial sugar binding site. N-bromosuccinamide analysis combined with 3 H-CB photolabeling implicates the region between Trp388 and Trp412 in ligand binding. Although its structure has been modeled (5) , the specific residues comprising the sugar binding site are unknown. A series of alanine point mutants were made, and mutant protein 2-deoxy glucose transport was tested in the presence of increasing [CB]. Arg126Ala and Cys421Ala GLUT1 mutations altered CB affinity but were determined not to be in the e1 site. The Arg400Ala mutation decreased binding affinity for CB, and may comprise part of the e1 binding site. Because point mutations were individually insufficient to abrogate CB binding, Trp388 to Trp412 chimeras were made. GLUT1/GLUT4 388-412 /GLUT1 and GLUT1/GLUT5 388-412 /GLUT1 chimeras showed moderately less sensitivity to CB inhibition of transport; these amino acids likely comprise regions determinant of CB binding affinity. Furthermore…
Microscale variation in alpine grasslands: AFLPs reveal a high level of genotypic diversity in Primula minima (Primulaceae)

The microscale variation and spatial genetic structure of the alpine plant species Primula minima L. was analysed using AFLPs. AFLP analysis based on three primer combinations and 123 fragments revealed no identical genotypes among the 86 studied samples from a 300 × 300 cm plot. Variation within the study plot was high: Nei's gene diversity was 0.22, Shannon's information index 0.33 and the percentage of polymorphic bands was 60.9. Cluster analysis revealed four main groups of genetically similar individuals and mapping these individuals resulted in a clear spatial pattern, with samples from the same group often located close together. The observed microscale structure was corroborated using a Mantel test, which revealed significant correlation of genetic and spatial distances, and by the results of a spatial autocorrelation analysis that indicated a high level of similarity between adjacent samples. An analysis of molecular variance revealed clear differentiation (18%) between the spatial groups. Overall gene flow within the plot was 1.11 and ranged from 0.33 between the spatially most distant groups to 2.33 between directly neighbouring groups. The extraordinary level of diversity detected in this study indicates an unexpectedly strong relevance of reproduction by seed for the species P. minima in alpine grasslands. The strong microscale variation suggests, however, that there is limited dispersal of seeds. Clonal reproduction is of subsidiary importance to sexual reproduction and seems to occur only over very small distances. © 2007 The Linnean Society of London, Botanical Journal of the Linnean Society, 2007, 155, 549–556.
Prevalence of various Î²-lactamase (ESBL, AmpC and MBL) producing multidrug resistant clinical isolates of Acinetobacter spp. in a tertiary care hospital.

Background: Extensive use of β- lactam group of antibiotics to treat the gram negative bacterial infections the antibiotic resistance are increasing and Acinetobacter species are budding as multidrug resistant (MDR) gram negative bacteria showing various β-lactamases mediated resistance. Materials and Methods: The presence of β-lactamases in the multi drug resistant clinical isolates of Acinetobacter species were screened by β-lactamase assay method. The presence of ESBL was detected by phenotypic confirmatory test recommended by Clinical and Laboratory Standards Institute ( CLSI ), AmpC was detected by CX/BA inhibitor method and carbapenem (IMP or MER)/EDTA combined disc method and Modified Hodge tests were performed to detect MBL mediated resistance mechanism. Results: We found high level of beta lactamase mediated resistance mechanism as part of multidrug resistance. Among 180 MDR isolates, 147 isolates were shown positive for beta lactamase assay method and these 147 isolates were further subjected to confirm presence of beta lactamase such as AmpC 105 (71.42%) followed by ESBL 84 (57.14%), MBL 43 (29.25%). Co-existence of ESBL and MBL also identified in 13 (8.84%) isolates. Conclusion: β-lactamase mediated resistance mechanisms are accounting very high in the multidrug resistant isolates of Acinetobacter species. Therefore early detection is necessary to screen and confirm beta lactamase mediated resistant strains to avoid treatment failure and prevent the spread of MDR.
Tingkat Kematangan Gonad Ikan Gabus (Channa striatus) di Rawa Sekitar Sungai Kelekar

Snakehead fish (Channa striata), one of fish species is economically in South Sumatera Province. This fish is raw material making food traditional in South Sumatera generally and Palembang city specially like empek-empek, laksan, tekwan, kerupuk-kemplang and others. During the time produce snakehead fish only coming from natural haul. The culture of this fish not yet because no availability technology of snakeheadfish culture. Research was conducted in Juli-Nopember 2005. The purpose of this research is to know gonad maturity level of snakeheadfish. research done by sampling in kelekar river floodplain area indralaya, ogan ilir. laboratory observation of sample done in aquaculture laboratory of agriculture faculty sriwijaya university. amount of obtained by fish sample is 25 fish, consist of 11 female fish and 14 male fish. male fish body is slimer than female fish. male fish body weight is 200-760 gram, and female fish body weight is 100-900 gram. from result of perception, gonad maturity level of snakehead fish is pregnant phase.
Global wetland outlook: Technical note on status and trends

The Status and Trends section of the Global Wetland Outlook (GWO) provides the context for the report by examining the distribution and condition of wetlands globally. This Technical Note provides further information on the area of wetlands globally. This is provided in support of the information on wetland area that has been summarised and included in the GWO. Further information on other measures used to describe the ecological character of wetlands is presented in the GWO and is not considered further in this Technical Note.
Response of nesting northern goshawks to logging truck noise in northern Arizona

We recorded 94 sound-response events at 3 adult-occupied northern goshawk (Accipiter gentilis) nests 78m, 143m, and 167m from the nearest United States Forest Service maintenance level 3, improved gravel road on the Kaibab Plateau in northern Arizona. During 4 test sessions on 7, 8, 10, and 11 June 2010, we recorded 60 experimentally controlled logging trucks; 30 non-experimental, civilian, fixed- winged aircraft; 3 non-experimental, passing cars; and 1 all-terrain vehicle (ATV). Logging truck sound, principally engine and exhaust, was concentrated in the 63Hz and 80Hz, 1/3-octave frequency bands. Aircraft sound was concentrated in the 100Hz and 125Hz, 1/3-octave frequency bands. Logging truck noise levels varied among nest sites and with distance from roads. At 167m from the closest road, the logging truck averaged approximately 8 decibels (dB, all decibel levels C-weighted, adjusted, 1s, equivalent sound level, CLEQ) quieter than aircraft. At 143m, the logging truck was approximately 3dB quieter than passing aircraft. Only at 78m was logging truck noiselouder on average than aircraft, by approximately 6dB. Average aircraft noise levels for each day of testing ranged between 45.6dB and 67.9dB and when averaged across the entire study period, varied little among the 3 test sites, 60.1-65.6dB. On average, our test logging truck (61.9dB) wasnolouderthanpassingaircraft (62.3dB),whichgoshawks generallyignored. The loggingtruck resulted in 27% no response and 73% alert (head turning) response, passing aircraft resulted in 90% no response and only 10% alert response, and the cars and ATV combined resulted in 50% each for no response and alert response. Goshawk alert response rates were inversely proportional to nest distance from the nearest road, decreasing from 91% to 57% alert between 78m and 167m. No movements or flushes were associated with either our experimental testing or passive recording of other vehicular noise stimuli. All tested pairs of goshawks successfully fledged young. Because logging truck noise had no effect on northern goshawks nesting along level 3, improved gravel roads on the Kaibab Plateau, restriction of log hauling and other road- based travel activities on similar roads within post-fledging family areas (PFAs) appears unnecessary based on the type and extent of our testing. 2013 The Wildlife Society.