Miracle-dz/UniFAR-FTUs huggingface dataset:
Identifying Important Time-Frequency Locations in Continuous Speech Utterances

Human listeners use speciﬁc cues to recognize speech and recent experiments have shown that certain time-frequency regions of individual utterances are more important to their correct identi-ﬁcation than others. A model that could identify such cues or regions from clean speech would facilitate speech recognition and speech enhancement by focusing on those important regions. Thus, in this paper we present a model that can predict the regions of individual utterances that are important to an automatic speech recognition (ASR) “listener” by learning to add as much noise as possible to these utterances while still permitting the ASR to correctly identify them. This work utilizes a continuous speech recognizer to recognize multi-word utterances and builds upon our previous work that performed the same process for an isolated word recognizer. Our experimental results indicate that our model can apply noise to obscure 90.5% of the spectrogram while leaving recognition performance nearly unchanged.
Removal of variable domain N-linked glycosylation as a means to improve the homogeneity of HIV-1 broadly neutralizing antibodies

ABSTRACT Broadly neutralizing antibodies are showing promise in the treatment and prevention of HIV-1, with several now being evaluated clinically. Some lead clinical candidates, including antibodies CAP256-VRC26.25, N6, PGT121, and VRC07-523, have one or more N-linked glycosylation sequons in their variable domains (Fvs) from somatic hypermutation, and these glycans increase chemical heterogeneity, complicating the manufacture of these antibodies as products. Here we propose a general method to remove Fv glycans and use this method to develop engineered versions of these four antibodies with Fv glycans removed. When germline residues were introduced to remove each glycan, antibody properties between wild type and mutant were not significantly altered for CAP256-VRC26.25 and PGT121; however, germline mutants for N6 and VRC07-523 showed increased polyreactivity, which is known to correlate with unfavorable in vivo pharmacokinetics. To reduce polyreactivity induced by removal of Fv glycan, we mutated aromatic residues and arginines structurally proximal to the removed glycan and identified Fv glycan-removed variants with low polyreactivity for N6 and VRC07-523. Two such variants, N6-N72LCQ-R18LCD and VRC07-523-N72LCQ-R24LCD, showed thermostability, neutralization potency and breadth, and half-life in humanized FcRn mice that were similar to their wild-type Fv-glycosylated counterparts. The removal of Fv glycan and reduction of chemical heterogeneity were confirmed by liquid chromatography-mass spectrometry. With reduced heterogeneity, the Fv-glycan-removed variants developed here may have utility as products for treating or preventing infection by HIV-1.
Coagulation Changes during Central Hypovolemia across Seasons

Lower body negative pressure (LBNP) application simulates hemorrhage. We investigated how seasons affect coagulation values at rest and during LBNP. Healthy participants were tested in cold (November–April) and warm (May–October) months. Following a 30-min supine period, LBNP was started at −10 mmHg and increased by −10 mmHg every five minutes until a maximum of −40 mmHg. Recovery was for 10 min. Blood was collected at baseline, end of LBNP, and end of recovery. Hemostatic profiling included standard coagulation tests, calibrated automated thrombogram, thrombelastometry, impedance aggregometry, and thrombin formation markers. Seven men (25.0 ± 3.6 years, 79.7 ± 7.8 kg weight, 182.4 ± 3.3 cm height, and 23.8 ± 2.3 kg/m2 BMI) and six women (25.0 ± 2.4 years, 61.0 ± 8.4 kg weight, 167 ± 4.7 cm height, and 21.8 ± 2.4 kg/m2 BMI) participated. Baseline levels of prothrombin (FII), tissue factor (TF) and markers for thrombin generation F1+2 and the thrombin/antithrombin complex (TAT) were higher during summer. Factor VIII, prothrombin fragment 1+2 (F1+2), TAT and the coagulation time showed significant increases during LBNP in both seasons. Some calibrated automated thrombography variables (Calibrated automated thrombography (CAT): lag, time to peak (ttPeak), peak) shifted in a procoagulant direction during LBNP in summer. Red blood cell counts (RBC), hemoglobin and white blood cell counts (WBC) decreased during LBNP. LBNP application reduced prothrombin time in winter and activated partial thromboplastin time in summer. Greater levels of FII, TF, F1+2, and TAT—a more pronounced LBNP-induced procoagulative effect, especially in CAT parameters (lag time (LT), Peak, ttPeak, Velindex)—were seen in summer. These results could have substantial medical implications.
Predicting Unacceptable Pain in Cardiac Surgery Patients Receiving Morphine Maintenance and Rescue Doses: A Model-Based Pharmacokinetic-Pharmacodynamic Analysis.

BACKGROUND Optimal analgesic treatment following cardiac surgery is crucial for both patient comfort and successful postoperative recovery. While knowledge of both the pharmacokinetics and pharmacodynamics of analgesics is required to predict optimal drug dosing, models quantifying the pharmacodynamics are scarce. Here, we quantify the pharmacodynamics of morphine by modeling the need for rescue morphine to treat unacceptable pain in 118 patients after cardiac surgery. METHODS The rescue morphine event data were analyzed with repeated time-to-event (RTTE) modeling using NONMEM. Postoperative pain titration protocol consisted of continuous morphine infusions (median duration 20.5 hours) with paracetamol 4 times daily and rescue morphine in case of unacceptable pain (numerical rating scale ≥4). RESULTS Patients had a median age of 73 years (interquartile range [IQR]: 63-77) and median bodyweight of 80 kg (IQR: 72-90 kg). Most patients (55%) required at least 1 rescue morphine dose. The hazard for rescue morphine following cardiac surgery was found to be significantly influenced by time after surgery, a day/night cycle with a peak at 23:00 (95% confidence interval [CI], 19:35-02:03) each day, and an effect of morphine concentration with 50% hazard reduction at 9.3 ng·mL (95% CI, 6.7-16). CONCLUSIONS The pharmacodynamics of morphine after cardiac surgery was successfully quantified using RTTE modeling. Future studies can be used to expand the model to better predict morphine's pharmacodynamics on the individual level and to include the pharmacodynamics of other analgesics so that improved postoperative pain treatment protocols can be developed.
Research of TPU Materials for 3D Printing Aiming at Non-Pneumatic Tires by FDM Method

3D printing technology has been widely used in various fields, such as biomedicine, clothing design, and aerospace, due to its personalized customization, rapid prototyping of complex structures, and low cost. However, the application of 3D printing technology in the field of non-pneumatic tires has not been systematically studied. In this study, we evaluated the application of potential thermoplastic polyurethanes (TPU) materials based on FDM technology in the field of non-pneumatic tires. First, the printing process of TPU material based on fused deposition modeling (FDM) technology was studied through tensile testing and SEM observation. The results show that the optimal 3D printing temperature of the selected TPU material is 210 °C. FDM technology was successfully applied to 3D printed non-pneumatic tires based on TPU material. The study showed that the three-dimensional stiffness of 3D printed non-pneumatic tires is basically 50% of that obtained by simulation. To guarantee the prediction of the performance of 3D printed non-pneumatic tires, we suggest that the performance of these materials should be moderately reduced during the structural design for performance simulation.
On the Design of Dual-Polarised Linear Antenna Arrays with Enhanced Port-to-Port Isolation

The paper describes the process of designing a dual-polarised linear antenna array with enhanced port-to-port isolation, with the example of a four-element array with isolation better than 60 dB for the U-NII 5.15–5.925 GHz band. As a single antenna, dual-polarised electromagnetically coupled microstrip antennas were used with port-to-port isolation not exceeding 25 dB. A significant improvement in the isolation of the array was achieved thanks to the application of a dedicated feeding network. On the basis of theoretical analysis, a mathematical model describing port-to-port isolation was developed. Circuit and full-wave simulations were carried out to show the influence of electromagnetic couplings between antennas and/or the microstrip lines of the feeding network and the selection of phase shifters/power dividers on the isolation. The fabricated prototype is characterised by a gain of about 14 dBi, polarisation purity of at least −27 dB within the main lobe and close to expectations isolation better than 57 dB within the whole operational band.
LVCSR with Transformer Language Models

Neural network language models (LMs) based on self-attention have recently outperformed the previous state of the art, LSTM LMs. Transformer LMs today are often used as a postprocessing step in lattice or n -best list rescoring. In this work the main focus is on using them in one-pass recognition. We show that by a simple reduction of redundant computations in batched self-attention we can obtain a 15% reduction in overall RTF on a well-tuned system. We also show that through proper initialization the layer normalization inside the residual blocks can be removed, yielding a further increase in forwarding speed. This is done under the constraint of staying close to state-of-the-art in terms of word-error rate (5.4% on LibriSpeech test-other) and achieving a real-time factor of around 1. Last but not least we also present an approach to speed up classic push-forward rescoring by mixing it with n -best list rescoring to better utilize the inherent parallelizability of Transformer language models, cutting the time needed for rescoring in half.
The prion-like phenomenon in Alzheimer’s disease: Evidence of pathology transmission in humans

1 Department of Neurology, McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, Texas, United States of America, 2 Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, University of Malaga, Malaga, Spain, 3 Department of Neuroscience, Baylor College of Medicine, Houston, Texas, United States of America, 4 Centro Integrativo de Biologı́a y Quı́mica Aplicada (CIBQA), Universidad Bernardo O’Higgins, Santiago, Chile
Poetic Meter Classification Using i-Vector-MTF Fusion

In this paper, a deep neural network (DNN)-based poetic meter classiﬁcation scheme is proposed using a fusion of musical texture features (MTF) and i-vectors. The experiment is performed in two phases. Initially, the mel-frequency cepstral co-efﬁcient (MFCC) features are fused with MTF and classiﬁcation is done using DNN. MTF include timbral, rhythmic, and melodic features. Later, in the second phase, the MTF is fused with i-vectors and classiﬁcation is performed. The performance is evaluated using a newly created poetic corpus in Malayalam, one of the prominent languages in India. While the MFCC-MTF/DNN system reports an overall accuracy of 80.83%, the i-vector/MTF fusion reports an overall accuracy of 86.66%. The performance is also compared with a baseline support vector machine (SVM)-based classiﬁer. The results show that the architectural choice of i-vector fusion with MTF on DNN has merit in recognizing meters from recited poems.
The Arabidopsis thaliana mRNA decay factor PAT1 functions in osmotic stress responses and decaps ABA‐responsive genes

mRNA decapping plays essential roles in regulating gene expression during cellular reprogramming in response to developmental and environmental cues. The evolutionarily conserved PAT1 proteins activate decapping by binding mRNA, recruiting other decapping components, and promoting processing body (PB) assembly. Arabidopsis encodes 3 PAT proteins: PAT1, PATH1, and PATH2. Here, we report that only pat1 mutants exhibit hypersensitivity to ABA and that transcripts of ABA‐responsive genes, but not those of ABA biosynthesis genes, persist longer in these mutants. The pat1 mutants also exhibit increased resistance to drought stress and resistance to Pythium irregulare. This is supported by assays showing that PAT1 functions specifically in decapping of the canonical ABA‐responsive gene COR15A. In summary, PAT1 protein mediates decay of ABA‐responsive genes and, thus, regulates stress responses.
Self-Supervised Adversarial Multi-Task Learning for Vocoder-Based Monaural Speech Enhancement

In our previous study, we introduce the neural vocoder into monaural speech enhancement, in which a ﬂow-based generative vocoder is used to synthesize speech waveforms from the Mel power spectra enhanced by a denoising autoencoder. As a result, this vocoder-based enhancement method outperforms several state-of-the-art models on a speaker-dependent dataset. However, we ﬁnd that there is a big gap between the enhancement performance on the trained and untrained noises. Therefore, in this paper, we propose the self-supervised adversarial multi-task learning (SAMLE) to improve the noise generalization ability. In addition, the speaker dependence is also evaluated for the vocoder-based methods, which is important for real-life applications. Experimental results show that the proposed SAMLE further improves the enhancement performance on both trained and untrained noises, resulting in a better noise generalization ability. Moreover, we ﬁnd that vocoder-based enhancement methods can be speaker-independent through a large-scale training.
InsB9-23 Gene Transfer To Hepatocytes-Based Combined Therapy Abrogates Recurrence of Type-1 Diabetes After Islet Transplantation.

The induction of antigen (Ag)-specific tolerance represents a therapeutic option for autoimmune diabetes. We demonstrated that administration of lentiviral vector enabling expression of insulinB9-23 (LV.InsB) in hepatocytes, arrests β cell destruction in pre-diabetic NOD mice, by generating InsB9-23-specific FoxP3+T regulatory cells (Tregs). LV.InsB in combination with a suboptimal dose of anti-CD3 mAb (combined therapy, 1X5µg CT5) reverts diabetes and prevents recurrence of autoimmunity following islets transplantation in ∼50% of NOD mice. We investigated whether CT optimization could lead to abrogation of recurrence of autoimmunity. Therefore, allo-islets were transplanted after optimized CT tolerogenic conditioning (1X25µg CT25). Diabetic NOD mice conditioned with CT25 when glycaemia was <500mg/dL, remained normoglycaemic for 100 days after allo-islets transplantation, displayed reduced insulitis, but independently from the graft. Accordingly, cured mice showed T cell unresponsiveness to InsB9-23 stimulation and increased Tregs frequency in islets infiltration and pancreatic LN. Additional studies revealed a complex mechanism of Ag-specific immune regulation driven by CT25, in which both Tregs and PDL1 co-stimulation cooperate to control diabetogenic cells, while transplanted islets play a crucial role, although transient, recruiting diabetogenic cells. Therefore, CT25 before allo-islets transplantation represents an Ag-specific immunotherapy to resolve autoimmune diabetes in the presence of residual endogenous β cell mass.
Mechanosensory Signaling in Astrocytes

Mechanosensitivity is a well-known feature of astrocytes, however, its underlying mechanisms and functional significance remain unclear. There is evidence that astrocytes are acutely sensitive to decreases in cerebral perfusion pressure and may function as intracranial baroreceptors, tuned to monitor brain blood flow. This study investigated the mechanosensory signaling in brainstem astrocytes, as these cells reside alongside the cardiovascular control circuits and mediate increases in blood pressure and heart rate induced by falls in brain perfusion. It was found that mechanical stimulation-evoked Ca2+ responses in astrocytes of the rat brainstem were blocked by (1) antagonists of connexin channels, connexin 43 (Cx43) blocking peptide Gap26, or Cx43 gene knock-down; (2) antagonists of TRPV4 channels; (3) antagonist of P2Y1 receptors for ATP; and (4) inhibitors of phospholipase C or IP3 receptors. Proximity ligation assay demonstrated interaction between TRPV4 and Cx43 channels in astrocytes. Dye loading experiments showed that mechanical stimulation increased open probability of carboxyfluorescein-permeable membrane channels. These data suggest that mechanosensory Ca2+ responses in astrocytes are mediated by interaction between TRPV4 and Cx43 channels, leading to Cx43-mediated release of ATP which propagates/amplifies Ca2+ signals via P2Y1 receptors and Ca2+ recruitment from the intracellular stores. In astrocyte-specific Cx43 knock-out mice the magnitude of heart rate responses to acute increases in intracranial pressure was not affected by Cx43 deficiency. However, these animals displayed lower heart rates at different levels of cerebral perfusion, supporting the hypothesis of connexin hemichannel-mediated release of signaling molecules by astrocytes having an excitatory action on the CNS sympathetic control circuits. SIGNIFICANCE STATEMENT There is evidence suggesting that astrocytes may function as intracranial baroreceptors that play an important role in the control of systemic and cerebral circulation. To function as intracranial baroreceptors, astrocytes must possess a specialized membrane mechanism that makes them exquisitely sensitive to mechanical stimuli. This study shows that opening of connexin 43 (Cx43) hemichannels leading to the release of ATP is the key central event underlying mechanosensory Ca2+ responses in astrocytes. This astroglial mechanism plays an important role in the autonomic control of heart rate. These data add to the growing body of evidence suggesting that astrocytes function as versatile surveyors of the CNS metabolic milieu, tuned to detect conditions of potential metabolic threat, such as hypoxia, hypercapnia, and reduced perfusion.
Multi-Modality Matters: A Performance Leap on VoxCeleb

The information from different modalities usually compensates each other. In this paper, we use the audio and visual data in VoxCeleb dataset to do person veriﬁcation. We explored different information fusion strategies and loss functions for the audio-visual person veriﬁcation system at the embedding level. System performance is evaluated using the public trail lists on VoxCeleb1 dataset. Our best system using audio-visual knowledge at the embedding level achieves 0.585%, 0.427% and 0.735% EER on the three ofﬁcial trial lists of VoxCeleb1, which are the best reported results on this dataset. Moreover, to imitate more complex test environment with one modality corrupted or missing, we construct a noisy evaluation set based on VoxCeleb1 dataset. We use a data augmentation strategy at the embedding level to help our audio-visual system to distinguish the noisy and the clean embedding. With such data augmented strategy, the proposed audio-visual person veriﬁcation system is more robust on the noisy evaluation set.
The Influence of the Use of Polymer Lining within the Roller Press Gravity Feeder on Briquette Quality

When considering the operation of roller presses for the consolidation of fine-grained materials, the main problems are disturbances in the proper flow of the material and its bridging in gravity feeders. This is especially true for small and medium capacity presses, where the hoppers for dosing the material are narrow. This article presents innovative laboratory tests of the impact of using a polymer plate lining in the gravity feeder of a roller press. Polymer materials Polyacetal C (POM C) and Ultra-High-Molecular-Weight Polyethylene (UHMW-PE) were used for the tests. The influence of the use of plates on the material flow and quality of briquettes was investigated in comparison with the case where such plates were not used. The research showed an improvement in the flow of fine-grained materials in the feeder and an increase of the briquette strength indexes, as compared to those cases when polymer linings were not used in the feeder.
High bacterial and viral load in the upper respiratory tract of children in the Democratic Republic of the Congo

Background Respiratory pathogens including Streptococcus pneumoniae and Haemophilus influenzae, are implicated in the pathogenicity of acute lower respiratory infection (ALRI). These are also commonly found in both healthy and sick children. In this study, we describe the first data on the most frequent bacteria and viruses detected in the nasopharynx of children from the general population in the Eastern DR Congo. Methods From January 2014 to June 2015, nasopharyngeal samples from 375 children aged from 2 to 60 months attending health centres for immunisation or growth monitoring were included in the study. Multiplex real-time PCR assays were used for detection of 15 different viruses and 5 bacterial species and for determination of pneumococcal serotypes/serogroups in the nasopharyngeal secretions. Results High levels of S. pneumoniae were detected in 77% of cases, and H. influenzae in 51%. Rhinovirus and enterovirus were the most commonly found viruses, while respiratory syncytial virus (RSV) was rare (1%). Co-occurrence of both bacteria and viruses at high levels was detected in 33% of the children. The pneumococcal load was higher in those children who lived in a dwelling with an indoor kitchen area with an open fire, i.e. a kitchen with an open fire for cooking located inside the dwelling with the resultant smoke passing to the living room and/or bedrooms; this was also higher in children from rural areas as compared to children from urban areas or children not living in a dwelling with an indoor kitchen area with an open fire/not living in this type of dwelling. Immunization with 2–3 doses of PCV13 was associated with lower rates of pneumococcal detection. Half of the identified serotypes were non-PCV13 serotypes. The most common non-PCV13 serotypes/serogroups were 15BC, 10A, and 12F, while 5, 6, and 19F were the most prevalent PCV13 serotypes/serogroups. Conclusions The burden of respiratory pathogens including S. pneumoniae in Congolese children was high but relatively few children had RSV. Non-PCV13 serotypes/serogroups became predominant soon after PCV13 was introduced in DR Congo.
Environment Changes, Aflatoxins, and Health Issues, a Review

Crops contaminated by aflatoxins (AFs), the toxic and carcinogenic mycotoxins produced namely by Aspergillus flavus and Aspergillus parasiticus, have severe impacts on human health. Changes in temperature and water availability related to actual climate changes (increased temperature, heavy rainfalls, and droughts) are modulating factors of mould growth and production of mycotoxins. To protect human and animal health from the harmful effects caused by AFs, the development of a safe and effective multifaceted approach in combating food and feed contamination with AFs is necessary. This review aims to collect and analyze the available information regarding AF presence in food and feed to reinforce AF management and to prevent health issues related to the AF exposure in the light of actual climate changes.