Miracle-dz/UniFAR-FTUs huggingface dataset:
Impaired phosphate handling of renal allografts is aggravated under rapamycin-based immunosuppression.

BACKGROUND Impaired phosphate handling of the renal allograft is a common problem and of multifactorial origin. The aim of the study was to elucidate whether a rapamycin- or a mycophenolate-based immunosuppressive therapy aggravates the renal phosphate leak in kidney transplant recipients. METHODS Renal phosphate handling was determined in thirty-eight cadaveric allograft recipients, with good renal function at 8, 12, 20 and 28 weeks after transplantation. Nineteen patients (group 1) received triple immunosuppression with rapamycin, cyclosporine and prednisolone, nineteen other transplant recipients received mycophenolate mofetil, cyclosporine and prednisolone immunosuppression (group 2), and six healthy subjects (group 3) served as controls. After 12 weeks of stable graft function, group 1 patients were divided further into two subgroups. Ten patients were kept on their immunosuppressive regimen (group 1A), whereas the remaining nine randomly chosen subjects had their cyclosporine withdrawn; they were thus maintained on a dual immunosuppression regimen with prednisolone and a higher dosage of rapamycin (group 1B). RESULTS Renal phosphate reabsorption was significantly lower in group 1 at 8 and 12 weeks after transplantation as compared with groups 2 and 3. At 20 weeks after transplantation, patients with rapamycin-based immunosuppression (groups 1A and 1B) continued to exhibit hypophosphataemia and impaired renal phosphate handling. Group 1B had the lowest TmP/ GFR compared with all groups. At 28 weeks, renal phosphate reabsorption and plasma phosphate levels were no longer different between patient groups and controls. CONCLUSION These data suggest that rapamycin-based immunosuppression prolongs the phosphate leak of the allografted kidney, leading to low serum phosphate levels during the first weeks after transplantation.
Analysis of human immunodeficiency virus type 1 nef gene sequences present in vivo

The nef genes of the human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2) and the related simian immunodeficiency viruses (SIVs) encode a protein (Nef) whose role in virus replication and cytopathicity remains uncertain. As an attempt to elucidate the function of nef, we characterized the nucleotide and corresponding protein sequences of naturally occurring nef genes obtained from several HIV-1-infected individuals. A consensus Nef sequence was derived and used to identify several features that were highly conserved among the Nef sequences. These features included a nearly invariant myristylation signal, regions of sequence polymorphism and variable duplication, a region with an acidic charge, a (Pxx)4 repeat sequence, and a potential protein kinase C phosphorylation site. Clustering of premature stop codons at position 124 was noted in 6 of the 54 Nef sequences. Further analysis revealed four stretches of residues that were highly conserved not only among the patient-derived HIV-1 Nef sequences, but also among the Nef sequences of HIV-2 and the SIVs, suggesting that Nef proteins expressed by these retroviruses are functionally equivalent. The "Nef-defining" sequences were used to evaluate the sequence alignments of known proteins reported to share sequence similarity with Nef sequences and to conduct additional computer-based searches for similar protein sequences. A gene encoding the consensus Nef sequence was also generated. This gene encodes a full-length Nef protein that should be a valuable tool in further studies of Nef function.
The effect of different types of hysterectomy on urinary and sexual functions: a prospective study

A prospective observational study was designed to evaluate the effect of the different techniques of hysterectomy on urinary and sexual function. One hundred and eighty-seven women aged 29 – 73 years and admitted for hysterectomy for various indications were recruited to the study. Women presenting primarily with major uterine prolapse and those requiring radical hysterectomy were excluded. Patients underwent one of four different techniques of hysterectomy: total abdominal, vaginal, laparoscopic or subtotal. All patients completed a standardised questionnaire addressing urinary and sexual symptoms and underwent urodynamic testing using the Lectromed 6000 System (Lectromed, Letchworth, Herts, UK) before and 6 months after surgery. Out of 187 women, 184 (98.4%) had completed data. Seventy-three patients (39%) had a total abdominal hysterectomy, 62 (34%) had vaginal, 38 (21%) had laparoscopic and 11 (6%) had subtotal hysterectomy. At 6 months after surgery, urinary symptoms occurred less frequently (P < 0.01) and urodynamic studies remained unchanged. Moreover, patients reported significantly lower rates of stress incontinence (P = 0.005), urgency (P = 0.03) and deep dyspareunia (P < 0.001) than before the operation, regardless of the hysterectomy technique used. The route of hysterectomy did not influence the outcome of surgery. We conclude that simple hysterectomy, whether performed abdominally, vaginally or laparoscopically, does not adversely affect urinary or sexual function at 6 months after surgery.
A multilingual mass communication intervention for seniors and people with disabilities on Medicaid: a randomized controlled trial.

OBJECTIVES To evaluate the following: (1) baseline knowledge, attitudes, and behavioral intentions about Medicaid managed care (MMC) among seniors and people with disabilities (SPD) receiving Medicaid benefits; (2) SPD Medicaid beneficiaries' use of and satisfaction with a user-designed MMC guidebook; and (3) guidebook effects on changes in MMC knowledge, attitudes, and intended behaviors of SPD beneficiaries. DATA SOURCES/STUDY SETTING Survey data collected between February and May 2008 from a random sample of SPD receiving Medicaid benefits in three California counties. STUDY DESIGN This randomized controlled trial of 319 intervention and 373 control SPD Medicaid beneficiaries used pre- and postintervention telephone surveys to compare changes in MMC knowledge, attitudes, and intended behaviors. DATA COLLECTION METHODS Baseline and follow-up telephone interviews were conducted in English, Spanish, Cantonese, and Mandarin. PRINCIPAL FINDINGS Seventy-seven percent of intervention participants reported using the guidebook. Nearly all (97.9 percent) found it somewhat or very useful. Intervention participants showed gains in knowledge, positive attitudes, and intentions to enroll in MMC that are statistically significant compared with control participants. However, knowledge levels remained low even among intervention participants. CONCLUSIONS Findings suggest that the guidebook is an effective way to improve recipients' MMC knowledge, confidence, and behavioral intentions.
Relationship of hemodialysis access to finger gangrene in patients with end-stage renal disease.

OBJECTIVE We report a comprehensive review of our patients on hemodialysis with end-stage renal disease (ESRD) with finger gangrene to determine etiology, natural history, and prognosis of this condition. METHODS Patients with ESRD with finger gangrene were identified from our computerized vascular registry. Presence of an ipsilateral arteriovenous fistula was determined, and patients were compared with a group of patients with ESRD without finger gangrene. Management consisted of arteriography, selective arteriovenous fistula management, and finger amputation. A multivariate analysis to determine risk factors associated with finger gangrene was performed. Repeat finger amputation and survival rates were determined with life-table analysis. RESULTS Twenty-three patients (mean age at start of dialysis, 53 years) with finger gangrene were identified, with 48% (n = 11) having a functional ipsilateral arteriovenous fistula. Arteriography was consistent with diffuse atherosclerosis involving the radial, ulnar, palmar, and digital arteries precluding attempts at distal arterial bypass. Repeat finger amputations were necessitated in 52% of patients (n = 12), and bilateral finger gangrene developed in 61% of patients (n = 14). Starting dialysis at age less than 55 years (P =.0004), diabetes (P =.001), coronary artery disease (P =.0212), and lower extremity arterial occlusive disease (P <.0001) were significantly associated with finger gangrene. CONCLUSION The young diabetic patient with diffuse vascular disease and ESRD is at high risk for the development of finger gangrene on chronic hemodialysis. Finger gangrene is the result of distal atherosclerosis and is not primarily related to dialysis access.
Routine Skin Preparation with 70% Isopropyl Alcohol Swab: Is itNecessary before an Injection? Quasi Study -

BACKGROUND: The world is facing economical crises and it has particularly affected third world countries. Therefore we must review the controversial health related procedures to save the cost and time without compromising health. The main goal of this study was to assess the need of skin preparation with 70% isopropyl alcohol swab wiping as an antiseptic measure, to prevent infections before intramuscular, subcutaneous and intradermal injections. DESIGN AND METHOD: Quasi experimental design. Microbiological as well as clinical assessment of pre-injection use of alcohol swabs was studied among the patients who need an injection at King Khalid University Hospital, Riyadh from August 2012 – December 2012. RESULTS: The mean CFU (Colony Forming Units) per ml over the injection site before alcohol swab wiping were significantly higher (2.47 ± 3.86) than after alcohol swab use (1.31 ± 1.93); p = 0.002. Although, 70% isopropyl alcohol swab reduced skin bacterial counts by 47%, there were no significant difference in clinical signs and adverse local or systemic effect with or without skin preparation by alcohol swab before intramuscular, intradermal or subcutaneous injections. CONCLUSION: Routine skin preparation with alcohol swab before an injection is quite unnecessary and is of no significant value in safeguarding infection. Omitting skin cleaning with alcohol swab prior to an injection would save time and money.
Double-blind, placebo-controlled, randomized, right-left study comparing calcipotriol monotherapy with a combined treatment of calcipotriol and diflucortolone valerate in chronic plaque psoriasis.

A double-blind, randomized clinical study was conducted to compare the efficacy and tolerability of twice-daily topical calcipotriol treatment with a combination treatment of calcipotriol once a day in the morning and diflucortolone valerate in the evening. Sixty-three patients with a clinical diagnosis of chronic plaque psoriasis and comparable psoriatic lesions on both sides of the body were included. After a washout phase of 1 week, psoriatic lesions were treated for 4 weeks with calcipotriol ointment twice daily on one side of the body and a combination of calcipotriol and diflucortolone valerate ointment on the other side. The treatment period was followed by a period of 4 weeks without any treatment. The psoriasis area and severity index (PASI) was used to compare the 2 groups. Furthermore, the overall therapeutic results were assessed independently by the investigators and by the patients. Both treatment regimens showed a significant, nearly identical, reduction in PASI. The mean PASI for calcipotriol alone was 5.7 at baseline, 1.9 after 4 weeks of treatment and 3.8 at the end of the follow-up period. For combination therapy, these values were 5.7, 1.8 and 3.8, respectively. There was a statistically significant advantage in favor of combined calcipotriol and diflucortolone valerate treatment at weeks 1 and 2 (p < 0.05); however, at the end of the treatment phase the difference between the 2 therapies was not significant. Subjective evaluation of efficacy by both the investigators and the patients revealed no difference between the 2 treatments. The frequency of side effects (e.g. irritation) was low in both groups. In conclusion, both therapies were effective for the treatment of chronic plaque-type psoriatic lesions. The combination of calcipotriol and a topical steroid appeared to produce a more rapid clinical response and was shown to be as effective as calcipotriol therapy alone.
Practice of Epidemiology Combining Matched and Unmatched Control Groups in Case-Control Studies

Multiple control groups in case-control studies are used to control for different sources of confounding. For example, cases can be contrasted with matched controls to adjust for multiple genetic or unknown lifestyle factors and simultaneously contrasted with an unmatched population-based control group. Inclusion of different control groups for a single exposure analysis yields several estimates of the odds ratio, all using only part of the data. Here the authors introduce an easy way to combine odds ratios from several case-control analyses with the same cases. The approach is based upon methods used for meta-analysis but takes into account the fact that the same cases are used and that the estimated odds ratios are therefore correlated. Two ways of estimating this correlation are discussed: sandwich methodology and the bootstrap. Confidence intervals for the pooled estimates and a test for checking whether the odds ratios in the separate case-control studies differ significantly are derived. The performance of the method is studied by simulation and by applying the methods to a large study on risk factors for thrombosis, the MEGA Study (1999–2004), wherein cases with first venous thrombosis were included with a matched control group of partners and an unmatched population-based control group.
A cross-university initiative to enhance SOTL through a students as partners approach

This case study evaluates a new initiative to establish a cross-disciplinary forum focusing on enhancing learning, teaching and the student experience. All staff and students are welcome to participate and participants set the agenda themselves. The intention is to have open and informal dialogue and to work in partnership towards setting up collective participatory action-research projects. This is modelled on the Teaching and Learning Academy at Western Washington University (Werder and Otis, 2010). An important aim was to create a space to give voice for those - the so-called ‘hard to reach’- who do not get such opportunities in traditional structures. There have been many challenges to creating a sustainable and successful working model, not least such barriers as communications, creating time and opportunity and working against current dominant cultures. Nonetheless, staff and students, including many international students, have participated and found legitimacy to discuss their own priorities.
Structure and function of the arolium of Mantophasmatodea (Insecta)

All species of the insect order Mantophasmatodea characteristically keep the 5th tarsomere and pretarsus (arolium plus two claws) turned upwards and off the substrate. The unusually large arolium was studied in two species of Mantophasmatodea using bright field light microscopy, reflection microscopy, fluorescence microscopy, TEM, SEM, and Cryo‐SEM. It contains an epithelial gland, numerous tracheoles, and nerves. The gland consists of enlarged epithelial cells with large nuclei, mitochondria, RER, golgi complexes, microtubules, and numerous secretion vesicles. Evidence for exocytosis of the vesicles into the gland reservoir between the epithelial gland and the thick cuticle could be observed. Cryo‐SEM revealed that the ventral side of the arolium and distal part of its dorsal side are covered with a liquid film. Fluid footprints of arolia of individuals walking on a glass plate also indicate the presence of secretory fluid on the arolium surface. Behavioral experiments using animals with ablated arolia showed that representatives of Mantophasmatodea do not need their arolia to detect and respond to vibratory communication signals nor to catch small to medium‐sized prey. Individuals with ablated arolia were not able to move upside down on a smooth glass plate. We conclude that Mantophasmatodea use their arolia for attachment when additional adhesion force is required (e.g. windy conditions, handling large prey, mating). They can bring their arolia in contact with the surface in a very fast reflex (18.0 ± 9.9 ms). The secretory fluid found on the surface is produced by the gland and transported to the outside, presumably through small pore channels, to enhance adhesion to the substrate. J. Morphol. , 2009. © 2009 Wiley‐Liss, Inc.
Functional Activity of Antibodies against the Recombinant OpaJ Protein from Neisseria meningitidis

ABSTRACT The opacity proteins belong to the major outer membrane proteins of the pathogenic Neisseria and are involved in adhesion and invasion. We studied the functional activity of antibodies raised against the OpaJ protein from strain H44/76. Recombinant OpaJ protein was obtained from Escherichia coli in two different ways: cytoplasmic expression in the form of inclusion bodies followed by purification and refolding and cell surface expression followed by isolation of outer membrane complexes (OMCs). Immunization with purified protein and Quillaja saponin A (QuilA) induced high levels of Opa-specific antibodies, whereas the E. coli OMC preparations generally induced lower levels of antibodies. Two chimeric Opa proteins, hybrids between OpaB and OpaJ, were generated to demonstrate that the hypervariable region 2 is immunodominant. Denatured OpaJ with QuilA induced high levels of immunoglobulin G2a (IgG2a) in addition to IgG1, whereas refolded OpaJ with QuilA induced IgG1 exclusively. These sera did not induce significant complement-mediated killing. However, all sera blocked the interaction of OpaJ-expressing bacteria to CEACAM1-transfected cells. In addition, cross-reactive blocking of OpaB-expressing bacteria to both CEACAM1- and CEA-transfected cells was found for all sera. Sera raised against purified OpaJ and against OpaJ-containing meningococcal OMCs also blocked the nonopsonic interaction of Opa-expressing meningococci with human polymorphonuclear leukocytes.
Time and Business Sustainability: Socially Responsible Investing in Swiss Banks and Insurance Companies

Business sustainability aims to combine market logic with social welfare logic. In literature, it is commonly assumed that sustainability and the social welfare logic associated with it are characterized by a long-term orientation. However, this assumption is problematic because this principle may not apply in certain contexts. This qualitative study challenges this assumption and focuses on the mechanisms by which time affects the adoption of sustainability practices in the context of socially responsible investing (SRI) practices in Swiss banks and insurance companies. The article provides insights into the mechanisms associated with different time horizons and investigates their effects on the adoption of SRI in financial intermediaries. It also shows how the dimension of time shapes interactions between the two institutional logics underlying SRI in business organizations through specific mechanisms.
The Hepatitis C Virus RNA-Dependent RNA Polymerase Membrane Insertion Sequence Is a Transmembrane Segment

ABSTRACT The hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp) belongs to a class of membrane proteins termed tail-anchored proteins. Here, we show that the HCV RdRp C-terminal membrane insertion sequence traverses the phospholipid bilayer as a transmembrane segment. Moreover, the HCV RdRp was found to be retained in the endoplasmic reticulum (ER) or an ER-derived modified compartment both following transient transfection and in the context of a subgenomic replicon. An absolutely conserved GVG motif was not essential for membrane insertion but possibly provides a docking site for transmembrane protein-protein interactions. These findings have important implications for the functional architecture of the HCV replication complex.
TELAAH KRITIS ATAS KONSEP GOOD CORPORATE GOVERNANCE DITINJAU DARI SHARI'ATE ENTERPRISE THEORY

GCG (Good Corporate Governance) is the most popular principle to keep integrity for organization in the world. Almost all countries in the world keep this principle for building accountability and transparency. Unfortunately, in the practice, GCG has less interpretation about the value of stakeholder. This research is aiming at reconstruction GCG (OECD version) and looking for the more acceptable concept for Islamic values. Hopefully, it can give solution for the problem itself. Having analyzed values and characters of SET, we construct new GCG based on shariaâ€™s values. These values are; first; understanding about stakeholder company more deeply, second; the principle about stakeholder rights; third; the equality about stakeholder behaviour; fourth; principle of transparency;fifth; about accountability of company.
Pollution profile and biodegradation characteristics of fur‐suede processing effluents

This study investigated the effect of stream segregation on the biodegradation characteristics of wastewaters generated by fur‐suede processing. It was conducted on a plant located in an organized industrial district in Turkey. A detailed in‐plant analysis of the process profile and the resulting pollution profile in terms of significant parameters indicated the characteristics of a strong wastewater with a maximum total COD of 4285 mg L−1, despite the excessive wastewater generation of 205 m3 (ton skin)−1. Respirometric analysis by model calibration yielded slow biodegradation kinetics and showed that around 50% of the particulate organics were utilized at a rate similar to that of endogenous respiration. A similar analysis on the segregated wastewater streams suggested that biodegradation of the plant effluent is controlled largely by the initial washing/pickling operations. The effect of other effluent streams was not significant due to their relatively low contribution to the overall organic load. The respirometric tests showed that the biodegradation kinetics of the joint treatment plant influent of the district were substantially improved and exhibited typical levels reported for tannery wastewater, so that the inhibitory impact was suppressed to a great extent by dilution and mixing with effluents of the other plants. The chemical treatment step in the joint treatment plant removed the majority of the particulate organics so that 80% of the available COD was utilized in the oxygen uptake rate (OUR) test, a ratio quite compatible with the biodegradable COD fractions of tannery wastewater. Consequently, process kinetics and especially the hydrolysis rate appeared to be significantly improved.
Ultrastructural observations on sinusoidal endothelial cells in chronic active hepatitis

Ultrastructurat features of 12 liver biopsies from patients with chronic active hepatitis were studied, particular attention being paid to endothelial cells. In areas of piecemeal necrosis and parenchymal inflammation sinusoidal endothelial cells show swelling of the cytoplasm, protrusion of the cell body into the sinusoidal lumen, increase in micropinocytotic vesicles and appearance of numerous dense bodies. This cell type is termed ‘active endothelial cell. Subsequent changes include enlargement of the Golgi complex, increase of rough endoplasmic reticulum in cytoplasmic processes with concomitant decrease of dense bodies, appearance of a fuzzy coat and formation of hemidesmosomes in close relationship to basement membrane‐like material and reticulin fibres in the space of Disse. The latter ultrastructural characteristics correspond to those of ‘fibroblastic reticulum cells’ described in lymph nodes. Active endothelial cells and fibroblastic reticulum cells may play a protective role in liver parenchymal inflammation by reducing the accessibility of noxious agents from the blood stream to liver parenchymal cells, and be crucial in the initiation of perisinusoidal fibrosis.