Miracle-dz/UniFAR-FTUs huggingface dataset:
The effect of cloprostenol on follicular development, ovulation, anovulation and pregnancy rate in the mare

7 LIST OF ORIGINAL ARTICLES . 9 ABBREVIATIONS. 10 INTRODUCTION. 11 REVIEW OF LITERATURE. 13 1. Prostaglandin F2α and its analogues. 13 1.1. Biological effects and pharmacokinetics. 13 1.2. Uses in equine reproduction . 14 1.3. Side effects following treatment with exogenous PGF . 19 2. Follicular dynamics . 22 2.1. Measurement of follicular diameter . 22 2.2. Follicular waves. 23 2.3. Diameter of the preovulatory follicle . 26 2.4. Multiple ovulation . 27 3. The ovulatory period . 29 3.1. Ultrasonographic signs of impending ovulation. 29 3.2. Hormonal mechanisms of the ovulatory process. 30 3.3. Ultrasonographic and clinical detection of ovulation. 30 3.4. Ovulatory failure. 32 AIMS OF THE STUDY. 38 MATERIALS AND METHODS . 39 1. Animals. 39 2. Ultrasonography . 39 2.1. Follicular diameter. 40 2.2. Ovulation and early corpus luteum. 40 2.3. Haemorrhagic anovulatory follicles . 41 2.4. Endometrial oedema. 41 2.5. Pregnancy diagnosis . 42 3. Hormonal and post-mating treatments . 42 3.1. Cloprostenol . 42 3.2. Human chorionic gonadotrophin . 43 3.3. Deslorelin (I) . 43 3.4. Post-mating treatments (III) . 43 4. Clinical diagnosis of the stage of the cycle . 43 4.1. Manual palpation of the cervix. 44 4.2. Ultrasonography . 44 5. Experimental design . 44 5.1. Repeatability of follicular diameter and endometrial oedema in individual mares (I). 44 5.2. Effect of cloprostenol dose and follicular diameter on the interval from treatment to ovulation (II) . 45 5.3. Effect of interval from treatment to ovulation on pregnancy and multiple ovulation rate (III) . 46 5.4. Effect of cloprostenol and human chorionic gonadotrophin on the occurrence of haemorrhagic anovulatory follicles (IV, V) . 47 6. Statistical analyses. 47 1. Repeatability of preovulatory follicular diameter (I) . 49 2. Repeatability of endometrial oedema pattern. 49
Distribution of low-molecular-weight glutenin subunit Glu-B3 alleles in mini core collections of Chinese wheat germplasms

Low-molecular-weight glutenin subunit (LMW-GS) Glu-B3 plays an important role in the processing quality of the wheat used to make bread and noodles. A total of 214 accessions of common wheat (Triticum aestivum L.) comprising both landraces and improved cultivars collected from all the three growing zones in China were tested for ten alleles using 10 allele-specific PCR markers at the Glu-B3 locus. The frequency of the 'I' allele (20.6%) was greater than those of the other alleles. The 'I' allele was found in 18.7% of landraces and 23.8% of improved cultivars. The alleles, from most to least common, were 'I'>'a'>'d'>'g'>'f'>'b'>'e'>'c'>'j'>'h. ' In the spring wheat zone, the Glu-B3 alleles were mainly 'g' and 'i,' but in the winter wheat zone, they were mainly 'a' and 'i. ' The 'h' allele was only found in the spring wheat zone, but the 'a' and 'c' alleles were found in small proportions in the spring wheat and spring-winter wheat zones. The frequency of the 'I' allele was more than twice that of the b allele (9.3%). Because the 'b' allele has a more pronounced effect on gluten strength, varieties with the 'b' allele can be considered suitable for the breeding of new, highquality cultivars.
Prediction model of air pollutant concentration based on deep neural network: A case study of Fushun, Liaoning Province

With the rapid development of the economy, the sources of air pollution are increasing, and the problem of air pollution is becoming more and more serious. Air quality prediction is a very effective means of predicting air pollution in the future, which helps the government regulatory authorities to provide early warning and protect people’s physical and mental health. In this paper, a prediction model of air pollutant concentration based on deep neural network is proposed. With the concentration of PM2.5 as the prediction target, the neural network chooses bidirectional Long Short-Term Memory (LSTM) and fully connected neural network. First, historical meteorological data and the PM2.5 density from 2016 to 2017 were used as training data in this paper, which were obtained from website. Then, after pre-processing the input data, the data is transmitted to the network and trained multiple times to obtain network parameters that make the prediction effect better. Next, the network model is applied to the test set, and the test results are compared with the actual values to measure the prediction effect. Finally, by comparing with other prediction models, the results show that the proposed model performs better and has higher accuracy.
Human Platelet Lysates as a Serum Substitute in Renal Epithelial Cell Culture

Cultured renal epithelia form monolayers of differentiated, polarized cells. On permeable supports, an apical and a basolateral compartment are separated by the cultured epithelium to study epithelial transport in vitro. However, culture conditions and culture media have a significant impact. In this respect, the quality of fetal bovine serum (FBS) seemed to be of major importance. The high lot‐to‐lot variablity of FBS was found to substantially influence the differentiation of epithelial cultures with regard to the generation of a transepithelial electrical resistance (TEER). Thus, batch‐testing of FBS was required. We recently reported on the use of human platelet lysates (PL) as a replacement for FBS. The growth promoting capacity of PL was tested on renal epithelial cell lines, for which differentiation end points are well established. PL support growth, proliferation and differentiation of LLC‐PK1, HK‐ 2 and MDCK cells. In addition, TEER was monitored in filter‐grown LLC‐PK1 and in MDCK II and MDCK I epithelia. Low‐resistance epithelia generated a TEER of 150–250 Ω•cm2 in PL‐media, as seen with FBS, and high‐resistance MDCK I retained their TEER of 8,000 Ω•cm2. PL are a valuable, animal‐derived component‐free substitute for FBS in renal epithelial cell culture with a proven high batch‐to batch uniformity.
TUG1 Promoted Tumor Progression by Sponging miR-335-5p and Regulating CXCR4-Mediated Infiltration of Pro-Tumor Immunocytes in CTNNB1-Mutated Hepatoblastoma

Introduction HB presents with the highest frequency of CTNNB1 mutations, resulting in activation of Wnt signaling pathway. A number of studies have demonstrated CTNNB1 mutation contributed to the development of HB. However, limited research explored the function of lncRNAs in HB with CTNNB1 mutation. Methods We screened lncRNA expression profiles in CTNNB1-mutated HB samples and identified lncRNAs associated with malignant phenotype in HB. The association between lncRNA and immune microenvironment was investigated. The biological function of lncRNA was further explored using in vitro experiments. Results TUG1 was identified as onco-lncRNA in CTNNB1-mutated HB. TUG1 was shown to be associated with the infiltration of pro-tumor immunocytes via regulating the expression of CXCR4, a chemokine receptor playing a critical role in regulation of immune microenvironment. Inhibiting TUG1 could increase endogenous levels of miR-335-5p and consequently downregulating CXCR4, a direct target of miR-335-5p. Conclusion Our findings provide evidence for TUG1 mediating infiltration of pro-tumor immunocytes in HB patients carrying CTNNB1 mutation. TUG1-miR-335-5p-CXCR4 axis might be a promising immunological target for the treatment of HB patients.
Expression profiling of rice AP2/EREBP genes responsive to abiotic stresses.

Objective) The AP2/EREBP transcription factors are known to be unique in plants, and they are involved in growth, development and signal transduction in many physiological and biochemical responses. In this study, all the rice AP2/EREBP genes were cloned and used to dissect their expression alteration responsive to different abiotic stresses, providing a foundation for exploring the mechanism of rice stress-resistance molecular regulation. (Method) A microarray gene chip was used to analyze the AP2/EREBP gene expression profiling in rice seedlings under the treatment of PEG, low-temperature, high-salt, ABA and GA. The expression patterns of selected differentially expressed genes were further identified by quantitative real-time PCR. (Result) Forty-two AP2/EREBP genes were detected differentially expressed under at least one abiotic stress by using a microarray chip containing the whole AP2/EREBP gene family, and the alteration of gene expression from array data was confirmed by qRT-PCR. Two AP2/EREBP genes were found differentially regulated at the same direction by all stresses; however each of the rest forty differentially expressed AP2/EREBP genes had the same or distinct expression pattern responsive to different abiotic stresses. (Conclusion) Two AP2/EREBP genes might play key molecular regulation roles responding to abiotic stresses in rice. The identified differentially expressed AP2/EREBP genes have the same or distinct molecular responsive mechanism to different abiotic stresses.
Lokal İleri Evre Rektum Kanserinde Pre-Operatif Radyoterapinin Yeri

\n Lokal ileri evre rektum kanseri (LIRK), onkolojide multidisipliner tedavi yaklasimlarinin ortak isbirligi sonucu etkili tedavi sonuclarin elde edilebildigi en iyi orneklerinden birisidir. Her ne kadar, radyoterapi ile kombine tedavi yaklasimlariyla hem lokal-sistemik rekurrensler hem de sagkalim acisindan basari oranlarinda artis olsa da LIRK’ de esas tedavi halen radikal cerrahidir. Gunumuzde preoperatif RT’ nin postoperatif RT’ ye gore daha ustun oldugu gosterilmistir. Bu derlemede preoperatif RT’ nin uygulanma yontemleri, avantaj ve dezavantajlari degerlendirmek amaclanmistir. \n
Discovery of a binary quasar at z = 1.76

We present the serendipitous discovery of a physical pair of quasars with estimated cosmological redshift z = 1.76. Long-slit spectroscopic observations of the candidate quasar GQ 1114+1549A were conducted with the OSIRIS instrument at the Gran Telescopio Canarias. The orientation of the slit allowed OSIRIS to also capture the spectrum of GQ 1114+1549B, an object with angular separation of ∆θ = 8.76 arcsec from GQ 1114+1549A. Spectral analysis on the Si iv and C iv, C iii] emission lines confirmed the quasar nature of GQ 1114+1549A, as well as that of its newly discovered companion. The relative intensity for the emission lines and the shape of the continuum of the two objects were found to be significantly different, rendering the hypothesis of the system being a gravitationally lensed quasar highly unlikely. The projected physical distance between the two quasars was therefore estimated to be 75 kpc.
Tax Loss Measurement

We use financial disclosures to develop a novel proxy for net operating loss carryforward (NOL) tax benefits. This approach more accurately identifies firms with tax losses, more precisely measures the tax loss, and better predicts reductions in future taxes than existing proxies. We derive a prediction model, which future researchers can employ to better approximate the NOL tax benefits based on readily available financial data. We also demonstrate how NOL nonlinearity affects measurement of corporate tax status. By proposing a new measure and demonstrating trade-offs across competing proxies, we contribute to the work in public economics, corporate finance, and tax accounting that examines the responsiveness of the business sector to corporate tax incentives.
Abundance and distribution of vegetation under four hardwood stands in north-central West Virginia

Forest floor samples were collected from four hardwood forest stands in West Virginia to study species composition, abundance, and distribution of vegetation that originated from seeds, rootstocks, rhizomes, and so on. The abundance and distribution of plants on square-foot sections of forest floor that were lifted and moved to the greenhouse indicate that under the most ideal conditions upwards of 800,000 stems per acre may occur during the first year. Sweet birch was the most abundant tree species, blackberry the most abundant shrub, and species of violets were the most abundant herbaceous plants. Implications of this vegetation of the regeneration of the forest are discussed.
Influence of habitat on the aggressive behavior of 13-line ground squirrels (Spermophilus tridecemlineatus).

13-lined ground squirrels (Spermophilus tridecemlineatus) are becoming more common on the campus of University of Michigan Biological Station (UMBS) in the northern Lower Peninsula of Michigan. We tested the hypothesis that this increase is due to areas of open habitat resulting from increased use of the UMBS environment by human residents. During the summer of 2014, we trapped and marked seven 13-lined ground squirrels. We observed the aggressive interactions between the ground squirrels with other species when competing for food. S. tridecemlineatus behaved more aggressively in open areas (Table 7: χ2= 10.011, df = 2, P ≥ 0.05) and dominated other diurnal squirrel species in them (Table 3: χ2 = 7.735, df = 1, P ≤ 0.05).
Low growth resilience of subarctic rhodoliths (Lithothamnion glaciale) to coastal eutrophication

Eutrophication is one of the most important drivers of change in coastal marine ecosystems worldwide. Given their slow growth, rhodoliths and the biodiverse communities they support are regarded as non-renewable resources threatened by human activity. Consequences of nutrient enrichment on growth and calcification in crustose coralline algae are equivocal, and even more so in cold-water rhodoliths. We paired a 183 d laboratory mesocosm experiment with a 193 d field experiment on Newfoundland (Canada) rhodoliths (Lithothamnion glaciale) to test the hypothesis that nutrient (nitrate, ammonia, and phosphate) enrichment and biofouling reduce rhodolith growth. Rhodoliths in the laboratory were exposed to 1 of 3 nutrient concentrations (ambient, intermediate, or high) and either of 2 levels of manual cleaning (cleaned or uncleaned) to control biofouling. We exposed rhodoliths in the field to 1 of 2 nutrient concentrations (ambient or elevated). Eutrophication in the laboratory did not affect biofouling; however, manual cleaning reduced biofouling by ~4 times relative to uncleaned rhodoliths. Rhodoliths grew 2 times slower at elevated than ambient nutrient concentrations, and ~27% more in cleaned than uncleaned rhodoliths at all concentrations. Rhodoliths in the field also grew significantly slower under elevated than ambient phosphate concentrations, but only during the first 6 wk, indicating some capacity for long-term recovery. We conclude that despite some growth resilience to low and infrequent increases in nutrient concentrations, subarctic L. glaciale rhodoliths cannot cope with prolonged exposure to modest eutrophication.
EFFECTS OF IRON AND COPPER DEFICIENCY ON THE EXPRESSION OF MEMBERS OF THE LIGHT‐HARVESTING FAMILY IN THE DIATOM THALASSIOSIRA PSEUDONANA (BACILLARIOPHYCEAE) 1

The LI818 proteins and their Lhcx homologs in diatoms are a subgroup of the light‐harvesting (LHC) antenna family, suspected of being involved in photoprotection and stress resistance. In this work, we report that the transcription of three LI818–like genes in Thalassiosira pseudonana Hasle et Heimdal (Lhcx1, Lhcx5, and Lhcx6) was down‐regulated under iron or copper deprivation and when both trace metals were limiting, as was the case for Lhcf4, one of the standard light‐harvesting genes. By contrast, the protein encoded by Lhcx1 was clearly up‐regulated under iron limitation, suggesting that this gene is independently regulated at transcriptional and translational levels. In general, copper starvation had less effect on the expression of light‐harvesting protein genes than iron deprivation, reflecting the different roles of iron and copper in photosynthetic function, that is, as an essential part of the electron transport chain versus as a cofactor for enzymes required to deal with the reactive oxygen species that result from inhibition of electron flow. Our results suggest that the Lhcx1 protein may be involved in stabilizing the photosynthetic apparatus when decreased nonphotochemical quenching (NPQ) results from Fe deficiency.
Effect of magnesium sulphate solution on compressive strength and sorptivity of blended concrete

This paper reports on the result of an experimental investigation carried out to study the compressive strength and sorptivity properties of blended cement concrete exposed to 5% and 10% MgSO4 solution using fly ash (FA) and silpozz. Usually in sulphate environment the minimum grade of concrete is M30 and the mix design is done for target mean strength of 39 MPa. Silpozz is manufactured by burning of agro-waste rice husk in designed furnace in between 600o to 700oC which is one of the main agricultural residues obtained from the outer covering of rice grains during the milling process. There are four mix series taken with control mix. The control mix made 0% replacement of FA and silpozz with Ordinary Portland Cement (OPC). The first mix series made 0% FA and 10-30% replacement of silpozz with OPC. The second mix series made with 10% FA and 10-40% replacement of silpozz with OPC. The third mix series made 20% FA and 10-30% replacement of silpozz with OPC and the fourth mix series made 30% FA and 10-20% silpozz replaced with OPC. The samples (cubes) are prepared and cured in normal water and 5% and 10% MgSO4 solution for 7, 28 and 90 days. The studied parameters are compressive strength and strength deterioration factor (SDF) for 7, 28 and 90 days. The water absorption and sorptivity tests have been done after 28 days of normal water and magnesium sulphate solution curing. The investigation reflects that the blended cement concrete incorporating FA and silpozz showing better resistance against MgSO4 solution when compared to normal water curing (NWC) samples.
The Pransky interview: Dr. Matanya Horowitz, founder and CEO of AMP robotics

This paper is a “Q&A interview” conducted by Joanne Pransky of Industrial Robot Journal as a method to impart the combined technological, business and personal experience of a prominent, robotic industry PhD-turned entrepreneur regarding his pioneering efforts of bringing technological inventions to market. The paper aims to discuss these issues. ,The interviewee is Dr Matanya Horowitz, Founder and CEO of AMP Robotics, an industrial artificial intelligence (AI) and Robotics Company that automates the identification, sorting and processing of complex waste streams to extract maximum value for their customers. Horowitz discusses how he came to found this groundbreaking company and created disruptive innovations. ,Horowitz earned four bachelor degrees in electrical engineering, computer science, applied mathematics and economics, as well as a master’s degree in electrical engineering, all from the University of Colorado. He also holds a PhD in control and dynamical systems from the California Institute of Technology with publications and research in control theory, AI, robotic path planning and computer vision. Shortly after finishing his PhD, he founded Louisville, Colorado-based AMP Robotics in 2014 with the mission of changing the fundamental economics of recycling. In the Fall of 2019, AMP raised $16m in Series A funding, led by Sequoia Capital. ,Horowitz developed and commercialized AMP’s breakthrough AI platform, AMP Neuron™ and robotics system, AMP Cortex™, which automates high-speed identification, sorting, picking and processing of material streams. Horowitz was individually recognized as Waste360’s “2019 Innovator of the Year” in addition to being named in their “40 under 40” list. AMP has received numerous awards and gained international recognition, including The Circulars 2018 Award for “Circular Economy Top Tech Disruptor” at the World Economic Forum in Davos, and the NWRA’s (National Waste and Recycling Association) “2017 Innovator of the Year” award.
PHENOLIC AND ANTIOXIDANT ACTIVITY OF BANGGAI SWEET POTATO ETHANOL EXTRACT (DIOSCOREA)

The aim of this study were to determine phenolic content and antioxidant activity of three varieties of banggai sweet. Research methods used the complete random methods on banggai sweet potato varieties of purple sweet potato, yellow sweet potato and white sweet potato with 5 times replication and to obtain 15 experiment units. The influence of ethanol ratio of banggai sweet potato flour and extraction time treatment produced extract phenolic content and influence varieties of banggai sweet potato phenolic content and activity of antioxidant applied to achieve the aim. The results shown that highest phenolic content (2,63%) was white banggai sweet potato, 2,44% of purple banggai sweet potato and 1,90% of yellow banggai sweet potato at ratio 15 : 1 based volume/weight (v/w) and time extraction at 2 hours. The highest activity of antioxidant ethanol extract (74,87%) was found at white banggai sweet potato extract and lowest activity of antioxidant (37,19%) was found at yellow banggai sweet potato. Antioxidant activity had relations with phenolic content. Antioxidant activity of 200 ppm BHT as control had no significant different with antioxidant activity of white sweet potato ekstract. compounds, carotenoids and anthocyanins in banggai sweet potato which act as natural antioxidants
Phytochrome, photosynthesis and flowering of Arabidopsis thaliana: photophysiological studies using mutants and transgenic lines

A number of phytochrome mutants have been examined for involvement in high irradiance (HIR) or red/far-red (R/FR) end-of-day (EOD) photoresponses during flowering of the long-day (LD) plant, Arabidopsis thaliana (L.) Heynh. A large component of phytochrome A (phyA) response is shown to involve an indirect effect via photosynthesis. When grown autotrophically in soil at a low irradiance (80 mol m–2 s–1), the phyA-211 mutant flowered extremely late compared with wild type and its leaf area was halved, both effects being reversed by increase in photosynthetic irradiance. Supplying sucrose via agar led to very early flowering with little indication of an additional direct phyA HIR. For light-stable phytochrome apoprotein mutants (phyB, phyD) or chromophore mutants (hy1, hy2), flowering was early and R/FR photoreversible EOD response was erased. Conversely, flowering was delayed in a transgenic line overexpressing the PHYB apoprotein. The FR EOD promotion of flowering via phyB was retained in darkness, brief night interruptions mimicking LD response. This novel finding emphasizes the importance of phyB-like phytochromes, with phyA acting indirectly. Whether phyB influences time measurement remains uncertain as we found no rhythmicity in this response to night interruptions. Overall, the role(s) of phytochromes in the regulation of flowering of Arabidopsis include EOD phyB-type response, a minor phyA photoperiodic response, and a large indirect phyA effect involving photosynthesis.
Iterative surgical demolition and reconstruction of the anterior chest wall and thoracic outlet for a recurrent chondrosarcoma: long term oncologic and functional results

Chondrosarcoma is a cartilage-producing neoplasm, with a preferential location in the chest wall at the level of the sternum and the ribs. Prognosis depends from several factors, such as tumour grading and radicality of resection. Due to chemo and radio resistance, surgery including radical removal of the tumour and reconstruction of the rib cage is the main treatment, with radiotherapy reserved in case of R1 or R2 resection. We report a case of a 56-year-old patient affected by sternocostal chondrosarcoma submitted to several chest wall resections and reconstruction due to the multiple relapses reporting surgical strategy, and its impact on quality of life and respiratory function.