Miracle-dz/UniFAR-FTUs huggingface dataset:
Sex Differences in Acute Swim Stress‐Induced Changes in the Binding of MK‐801 to the NMDA Subclass of Glutamate Receptors in Mouse Forebrain

Acute swim stress (3 min at 32°C) in mice produces increases in the binding of MK‐801 to the NMDA subclass of glutamate receptors to forebrain membranes prepared from male mice. Scatchard analyses indicate that the observed increases in the binding of MK‐801 in membranes from male mice are the result of changes in the affinity and density of low‐affinity binding sites and in the density of high‐affinity binding sites. In female mice, any changes in the binding of MK‐801 appear to be much less pronounced and restricted to the low‐affinity binding sites. These results are in contrast to the situation with binding to GABA receptors where acute swim stress increases GABA binding in forebrain membranes much more in female than in male mice. This indicates significant sex differences in the responses of receptors for the major excitatory and inhibitory transmitters to acute swim stress. These rapid changes in MK‐801 binding may result from changes in endogenous modulators as appears to be the case in the acute swim stress‐induced changes in GABA binding. As with GABA binding, the endogenous modulators are likely to include steroids, the sex differences reflecting differences in modulation by gonadal steroids and the stress‐induced changes reflecting differences in modulation by adrenal steroids. Estradiol, progesterone, and corticosterone treatments have been reported by other workers to influence the properties of glutamate receptors.
Carcase characteristics and qualitative meat traits of three Italian local chicken breeds

1. An experiment involving 60 male chickens reared in an organic production system was carried out in order to investigate carcase characteristics and qualitative meat traits of three slow-growing Italian local breeds of chicken (Ermellinata, Padovana and Pépoi). 2. Chicks were randomly selected at hatch, raised together under the same conditions, slaughtered at 190 d of age, dissected for carcase traits, and meat (breast and thigh) stored for subsequent analysis of quality parameters. 3. Ermellinata (EA) chickens were significantly different from Padovana (PA) and Pépoi (PI) chickens for live, carcase and thigh weights. Breeds were also different for breast muscle protein content (EA > PI and PA), shear force (PA < EA and PI) and cooking loss (PI > PA and EA) values. 4. The CIE system values of lightness (L*), redness (a*), and yellowness (b*) evidenced a distinctive darker meat and lighter skin colour of PA breast meat. 5. Polyunsaturated fatty acids composition of breast meat was similar among the analysed breeds. EA had significantly higher saturated but significantly lower monounsaturated fatty acid contents than the other two breeds.
Evaluation du pilotage de la performance extra financière des organisations: Cas de la Responsabilité sociale et environnementale des entreprises françaises du CAC40

Extra financial performance of organizations depends on the degree of incentive intensity of the institutional framework and the degree of voluntarism leaders in the selection and application of non-financial reporting standards. French companies traded, benefiting from an institutional incentive-intensive, which pushes agents to appropriate more or less a repository of reporting the social and environmental responsibility, do not improve their performance extra financial when the degree of political will of leaders decreases. The principle of voluntarism non-financial reporting is operating in an institutional incentive-intensive and low degree of voluntarism. Hence the relativity of Tetranormalization.
[Comparison of the effectiveness of quadruple salvage regimen for Helicobacter pylori infection according to the duration of treatment].

BACKGROUND/AIMS At present, triple therapy schemes are recommended by national and international consensus conferences for the treatment of Helicobacter pylori (H. pylori) infection. However, even with the most effective current treatment regimens, about 10-20% of patients fail to eradicate H. pylori, necessitating alternative strategy to eradicate H. pylori in primary treatment failure. Therefore, we performed this study to evaluate the efficacy of quadruple therapy and to compare 1 and 2-week quadruple regimen as a second-line therapy. METHODS The hospital records of 155 patients who failed to the standard triple therapy (proton pump inhibitor, amoxicillin, clarithromycin) were reviewed retrospectively, and divided the 1 or 2 weeks OBMT regimen (omeprazole 20 mg bid, bismuth salt 120 mg qid, metronidazole 500 mg tid, tetracycline 500 mg qid). Presence of H. pylori infection and side-effects of the treatment regimen were assessed 4 weeks after the cessation of treatment. RESULTS One hundred and eight male and 47 female (mean age, 52.2+/-15.4) patients were enrolled. The overall eradication rate of H. pylori with quadruple therapy was 83.9% and the eradication rate was similar between 1 and 2 weeks of OBMT regimen (76.8% in OBMT 1 week, 87.9% in OBMT 2 weeks, respectively p=0.110). CONCLUSIONS Quadruple therapy is an effective salvage regimen for H. pylori eradication after the failure of standard triple therapy. One week quadruple therapy is not significantly different from 2-weeks regimen as the second-line option for H. pylori eradication.
Prevalence of enterohemorrhagic Escherichia coli serotypes in Australian beef cattle.

OBJECTIVES Beef cattle are known reservoirs of Escherichia coli O157; therefore, it is possible that they may be reservoirs for other enterohemorrhagic E. coli (EHEC) serotypes. This study investigated the prevalence of EHEC serotypes O26, O45, O91, O103, O111, O121, O145, and O157 in 300 beef cattle fecal samples. MATERIALS A combination of 300 (140 grain-fed and 160 grass-fed) cattle fecal samples were collected post-evisceration from Australian abattoirs. Enriched samples were tested for the putative virulence markers stx₁, stx₂, and eae using real-time PCR. Samples that tested positive for stx and eae were then tested for the target serotypes. Isolation was performed on any sample testing positive to stx and eae, and a target serotype using immunomagnetic separation for serotypes O26, O103, O111, O145, and O157 or colony hybridization for serotypes where immunomagnetic separation beads were not commercially available (O45, O91, and O121). Resulting isolates were characterized for the presence of stx₁, stx₂, eae, and ehxA using a multiplex PCR. RESULTS Seventy-eight of the 300 samples (26%) were shown to contain stx and eae with 30 of these subsequently testing positive for the presence of at least one EHEC serotype. Of the 27 E. coli of EHEC serotypes isolated during the study, only 1 E. coli O91, 1 E. coli O26, and 5 E. coli O157 tested positive for the presence of any EHEC virulence markers. CONCLUSIONS This study found that the overall prevalence of EHEC in Australian beef cattle is very low. APPLICATIONS Testing for the presence of virulence determinants and O-specific genes alone will overestimate the presence of pathogenic serotypes in beef. Isolation of strains of interest and confirming the presence of virulence determinants in those strains should be an essential part of any test protocol.
INFLUENCE OF MODERATE PROPHYLACTIC COMPRESSION ON SPORT PERFORMANCE

This study examined the impact of using elasticized compression shorts on performance measures and proprioception at the hip. Thirteen healthy subjects completed 2 randomized testing sessions—one while wearing the Coreshorts compression shorts and one while not wearing the shorts. During each trial, active range of motion at the hip; joint angle replication during hip flexion, abduction, and hyperextension; leg power; agility; speed; and aerobic endurance were measured, and subjective information pertaining to the fit of the shorts was collected. The use of the prophylactic brace did not limit performance on any measure except active range of motion during hip flexion (p < 0.05). Subjective data revealed 93.3% of subjects felt the shorts were supportive, although proper fit was an issue. The present results support the use of moderate compression at and around the hip for the purpose of injury prevention. Continued research is necessary to determine the efficacy of hip bracing within an injured population and their potential prophylactic benefit for active individuals.
Implementation of diversification strategy at the Standard Group (K) Limited

Sustaining business growth is one of the key challenges to the business leader. Product diversification is one of a few answers to this problem. This study therefore sought to fill this knowledge gap by investigating the diversification strategies adopted by the Standard Group. The objectives of this study were to establish how the Standard group has implemented diversification strategies and to determine the factors influencing the implementation of diversification strategy at the Standard Group. This was a case study since the unit of analysis was one organization. Primarydata was used for the study through use of interview guides that were distributed among the senior managers in the Standard Group. Content analysis was employed in analyzing the data. The analysis was based on the objectives of the study. The qualitative analysis was used to analyze the respondents' views about the types of diversification strategies adopted by the Standard Group and their effect on the performance. This study established that the group adopts diversification strategies to maximize profits and compete effectively in the media market; diversification strategies are adopted to consolidate the company's market share and ward off competition from its rivals, so as to spread the risks occasionally by using cost of operation, to maximize on profits. From the study it s also concluded that the company employs operation such as multimedia house targeting different segments of the market. The other aspect of the company's market power was in reaching a wider market. The study therefore recommend that the organization should also incorporate diversification in its business operations through use of technological advancements and other aspects such as innovation and benchmarking strategies to realize full benefits of diversification. The study recommends that the company should work towards leading the media industry by setting up strategic advisory
mRNA Profiling for Body Fluid Identification by Multiplex Quantitative RT‐PCR *

Abstract: An alternative approach to conventional protein‐based body fluid identification is gene expression profiling analysis. In the present work, we report the development of sensitive and robust multiplex quantitative reverse transcriptase‐PCR assays for the identification of blood, saliva, semen, and menstrual blood. Each body fluid assay comprises a triplex system that detects transcripts from two body fluid‐specific genes and a housekeeping gene GAPDH. The body fluid‐specific genes include erythroid δ‐aminolevulinate synthase (ALAS2) and β‐spectrin (SPTB) for blood, statherin (STATH) and histatin 3 (HTN3) for saliva, protamine 1 (PRM1) and protamine 2 (PRM2) for semen, and matrix metalloproteinase 7 (MMP7) and matrix metalloproteinase 10 (MMP10) for menstrual blood. Normalization of both body fluid‐specific genes to the housekeeping gene by means of appropriate cycle threshold metrics ensures the high specificity of each assay for its cognate body fluid.
A reference model for interaction semantics

Understanding the issue of semantic interoperability will be key in the design of future IoT-devices and cyber physical systems. In this article, we introduce a reference model for the interaction semantics of networking systems to provide the basis for such an understanding. The notions of system, function, information, action, event, interaction, process and meaning are defined in a well suited way bridging the gap between the world of physics, information and meaning. We apply the reference model to classify interaction interfaces and components as well as to define the software layer and the role concept. This provides important elements of a reference architecture to design interoperable IoT-devices and cyber physical systems more easily.
Naval Multi-Function RADAR

The missions expected to be covered by Naval Multi-Function RADAR (MFR) have increased significantly these last decades. The main evolution of these systems, from a design point of view, have two origins, one being the Tactical Picture Compilation (TPC), the other being the extension of the warfare domain of weapon systems connected to MFR. As far as TPC is concerned, Long Range coverage and proximity of littoral, have been the major requirements impacting the design of naval MFR. This paper discusses the principal features of typical architecture for modern Naval Multi-Function RADAR, and how the requested new missions impact the design.
System architecture of an all-digital GHz transmitter using pulse-width/position-modulation for switching-mode PAs

This paper presents a novel concept for direct-pulse-width/position-modulation (PWPM) in an all-digital transmitter by using a parallel baseband PWPM and high speed digital up-conversion. By parallel calculation and subsequent serialization of the digital PWPM signal carrier frequencies above 1 GHz become feasible. PWPM enables the usage of highly efficient switching-mode power amplifiers (SMPA) and direct control of the output power. Because PWPM modulator can be completely implemented in the digital, time discrete domain, all analogue baseband blocks can be dropped and replaced by reconfigurable digital blocks. The only remaining mixed-signal and analogue blocks are the multiplexer and the power amplifier. Requirements on the digital and mixed-signal blocks are discussed. Feasibility of the concept is demonstrated by system and circuit simulations showing the possibility of using digital PWPM in wireless applications.
Improving MIMO system capacity by compensating mutual coupling in transmitting/receiving array antennas

We make use of the inverse coupling matrix to achieve mutual coupling compensation for transmitter and receiver antenna arrays to improve the performance of a MIMO system. Using this approach, we assess the MIMO capacity as a function of spacing and number of elements in the receiving and transmitting antenna arrays. Our results reveal that the MIMO capacity is reduced when mutual coupling effects become pronounced. It is shown that the effect of coupling can be compensated using the information obtained from the mutual impedance matrices for transmitting/receiving arrays. The MIMO capacity can be significantly increased by compensating mutual coupling effects at one side or both sides of transmission. When both sides are compensated, the capacity becomes almost the same as for an ideal system in which mutual coupling is absent or can be fully neglected.
Acid adaptation promotes survival of Salmonella spp. in cheese

Salmonella typhimurium was adapted to acid by exposure to hydrochloric acid at pH 5.8 for one to two doublings. Acid-adapted cells had increased resistance to inactivation by organic acids commonly present in cheese, including lactic, propionic, and acetic acids. Recovery of cells during the treatment with organic acids was increased 1,000-fold by inclusion of 0.1% sodium pyruvate in the recovery medium. Acid-adapted S. typhimurium cells survived better than nonadapted cells during a milk fermentation by a lactic acid culture. Acid-adapted cells also showed enhanced survival over a period of two months in cheddar, Swiss, and mozzarella cheeses kept at 5 degrees C. Acid adaptation was found in Salmonella spp. , including Salmonella enteritidis, Salmonella choleraesuis subsp. choleraesuis serotype heidelberg, and Salmonella choleraesuis subsp. choleraesuis serotype javiana, associated with food poisoning. These observations support the theory that acid adaptation is an important survival mechanism enabling Salmonella spp. to persist in fermented dairy products and possibly other acidic food products.
Fostering Serendipitous Knowledge Discovery using an Adaptive Multigraph-based Faceted Browser

Serendipity, the art of making an unsought finding plays also an important role in the emerging field of data science, allowing the discovery of interesting and valuable facts not initially sought for. Previous research has extracted many serendipity-fostering patterns applicable to digital data-driven systems. Linked Open Data (LOD) on the Web which is powered by the Follow-Your-Nose effect, provides already a rich source for serendipity. The serendipity most often takes place when browsing data. Therefore, flexible and intuitive browsing user interfaces which support serendipity triggers such as enigmas, anomalies and novelties, can increase the likelihood of serendipity on LOD. In this work, we propose a set of serendipity-fostering design features supported by an adaptive multigraph-based faceted browsing interface to catalyze serendipity on Semantic Web and LOD environments.
Navigating the Transition From Cancer Care to Primary Care: Assistance of a Survivorship Care Plan.

PURPOSE/OBJECTIVES To examine symptom and quality-of-life (QOL) trajectories in breast cancer and lymphoma survivors enrolled in a survivorship navigation intervention and to explore patient, caregiver, and primary care provider (PCP) satisfaction with receipt of a survivorship care plan (SCP). DESIGN Prospective, cohort, longitudinal. SETTING The Billings Clinic, an integrated cancer center in Montana. SAMPLE 67 patients with breast cancer or lymphoma who recently completed cancer treatment, along with 39 of their caregivers and 23 PCPs. METHODS Data collection at one, three, and six months by the Functional Assessment of Cancer Therapy-General and satisfaction surveys. MAIN RESEARCH VARIABLES Symptoms, QOL, and satisfaction with the survivorship navigator and the SCP. FINDINGS Symptoms persisted six months following treatment. Symptoms and QOL indicators with worst intensity were energy, sleep, coping, and satisfaction with sex life. Patients with more comorbidities reported worse QOL, telephoned the survivorship navigator more often, and were more satisfied with the SCP. Patients with lymphoma reported higher QOL, but it was not significantly different from patients with breast cancer. Patients were significantly more satisfied than caregivers with the SCP at time 1. PCPs were highly satisfied with the SCP. CONCLUSIONS Some symptoms persist, even when cancer treatment has ended. Patients with comorbidities are at higher risk for more severe symptoms and worse QOL and may benefit from ongoing support. SCPs can facilitate patients' transition to primary care following cancer treatment. IMPLICATIONS FOR NURSING Healthcare professionals who care for breast cancer survivors need to routinely assess them for the presence of comorbid conditions. Obese breast cancer survivors may benefit from weight reduction interventions to possibly decrease their risk of developing lymphedema and improve their overall health status.
Segregation of Family A G Protein–Coupled Receptor Protomers in the Plasma Membrane

G protein–coupled receptors (GPCRs) transduce many important physiological signals and are targets for a large fraction of therapeutic drugs. Members of the largest family of GPCRs (family A) are thought to self-associate as dimers and higher-order oligomers, although the significance of such quaternary structures for signaling or receptor trafficking is known for only a few examples. One outstanding question is the physical stability of family A oligomers in cell membranes. Stable oligomers would be expected to move through cellular compartments and membrane domains as intact groups of protomers. Here, we test this prediction by recruiting subsets of affinity-tagged family A protomers into artificial microdomains on the surface of living cells and asking if untagged protomers move into these domains (are corecruited) at the same time. We find that tagged β2 adrenergic and μ-opioid protomers are unable to corecruit untagged protomers into microdomains. In contrast, tagged metabotropic glutamate receptor protomers do corecruit untagged protomers into such microdomains, which is consistent with the known covalent mechanism whereby these family C receptors dimerize. These observations suggest that interactions between these family A protomers are too weak to directly influence subcellular location, and that mechanisms that move these receptors between subcellular compartments and domains must operate on individual protomers.
Fate of Clots in Patients With Subarachnoid Hemorrhage After Different Surgical Treatment Modality: A Comparison Between Surgical Clipping and Guglielmi Detachable Coil Embolization

BACKGROUND:Subarachnoid clot is important in the development of delayed vasospasm after subarachnoid hemorrhage (SAH). OBJECTIVE:To compare the clearance of subarachnoid clot and the incidence of symptomatic vasospasm in surgical clipping and embolization with Guglielmi detachable coils for aneurysmal SAH. METHODS:The subjects were 115 patients with Fisher group 3 aneurysmal SAH on computed tomography scan at admission whose aneurysm was treated by surgical clipping (clip group; n = 86) or Guglielmi detachable coil embolization (coil group; n = 29) within 72 hours of ictus. Software-based volumetric quantification of the subarachnoid clot was performed, and the amount of hemoglobin in drained cerebrospinal fluid was measured. RESULTS:Clearance of the subarachnoid clot on the computed tomography scan was rapid in the clip group until the day after the operation but slow in the coil group (58.9% removed vs 27.8% removed; P = .008). However, postoperative clearance of the clot occurred more rapidly in the coil group. Reduction of the clot until days 3 through 5 did not differ significantly between the 2 groups (72.9% removed vs 75.2% removed). The amount of hemoglobin in the clip group was > 0.8 g/d until day 3 and then gradually decreased (n = 15), but hemoglobin in the coil group remained at > 0.8 g/d until day 5 (n = 17). The incidence of symptomatic vasospasm did not differ between the groups. CONCLUSION:Subarachnoid clot can be removed directly during surgical clipping, which is not possible with endovascular treatment. However, the percentage reduction of the clot on days 3 through 5 did not differ between the 2 groups.
Genetic modification of ER‐Hoxb8 osteoclast precursors using CRISPR/Cas9 as a novel way to allow studies on osteoclast biology

Osteoclasts are cells specialized in bone resorption. Currently, studies on murine osteoclasts are primarily performed on bone marrow–derived cells with the use of many animals and limited cells available. ER‐Hoxb8 cells are conditionally immortalized monocyte/macrophage murine progenitor cells, recently described to be able to differentiate toward functional osteoclasts. Here, we produced an ER‐Hoxb8 clonal cell line from C57BL/6 bone marrow cells that strongly resembles phenotype and function of the conventional bone marrow–derived osteoclasts. We then used CRISPR/Cas9 technology to specifically inactivate genes by biallelic mutation. The CRISPR/Cas9 system is an adaptive immune system in Bacteria and Archaea and uses small RNAs and Cas nucleases to degrade foreign nucleic acids. Through specific‐guide RNAs, the nuclease Cas9 can be redirected toward any genomic location to genetically modify eukaryotic cells. We genetically modified ER‐Hoxb8 cells with success, generating NFATc1−/− and DC‐STAMP−/− ER‐Hoxb8 cells that lack the ability to differentiate into osteoclasts or to fuse into multinucleated osteoclasts, respectively. In conclusion, this method represents a markedly easy highly specific and efficient system for generating potentially unlimited numbers of genetically modified osteoclast precursors.