Miracle-dz/UniFAR-FTUs huggingface dataset:
The Active Role of Spray-Dried Phytase Produced by Rhizopus microsporus var. microsporus Biofilm in Feeding Broiler Chickens

Objective: Phytases are enzymes that can catalyze the hydrolysis of phytic acid, and have potential for use in the formulation of broiler chickens’ diets. As such, we evaluated the effects of supplementation of broiler chicken diets with a spraydried phytase derived from the fungal Rhizopus microsporus var. microsporus biofilm on the animal performance. Methods: Cobb 500 male chicks were housed for 42 days under controlled environment conditions and provided with three different dietary regimes. These included a control diet meeting nutritional requirements, a diet with phosphorus reduction of 0.175% over days 1-21 and 0.225% over days 22-42, and diets with spray-dried phytase added at 250 FTU/kg, 500 FTU/kg and 750 FTU/kg. Animal performance was assessed based on their body weight, feed intake, weight gain, feed conversion rate, and viability. Results: At the first phase of development (1-21 days of age), performance was not improved by phytase addition, but at the latter phase (22-42 days of age); performance improved significantly, especially in birds fed phytase at 750 FTU/ kg. In addition, phytase promoted an increase in villous height for animals of both 21- and 42 day-old animals, improving nutrient absorption and performance at the end phase of development. Conclusion: Phytase supplementation positively affected animal development and jejunum morphology, highlighting its potential to be used as an additive for broiler chicken diet formulation.
Bodyweight BOSU Ball Exercises

A growing trend in strength and conditioning is the execution of traditional exercises on instability devices. With the addition of these implements, there is a greater challenge to maintain stability and balance. Implementing a device, such as the BOSU ball, may provide benefits to individuals rehabilitating from an injury, older individuals, and those looking to increase overall health benefits. Thus, this column provides detailed descriptions of the proper technique, advanced progressions, and exercise prescription for 4 bodyweight BOSU ball exercises.
Turist Duyguları, Turist Memnuniyeti, Destinasyon Aşkı ve Destinasyon Sadakati Arasındaki Yapısal İlişkinin Belirlenmesi: Bozcaada Örneği (Determination of the Structural Relationship Between Tourist Feelings, Tourist Satisfaction, Love for Destination and Destination Loyalty: The Case of Bozcaada)

Uluslararası turist sayılarında ve turizm gelirlerindeki artışla birlikte destinasyonlar arasındaki rekabet daha zorlu hale gelmiştir. Bu rekabet şartları içerisinde destinasyonlar tercih edilebilirliklerini artırmak için farklılaşmaları ve mevcut turizm pazarlarını korumaları gerekir. Bu durumun sağlanması için destinasyon sadakatinin oluşturulması ve destinasyon markasının yaratılması çok önemlidir. Bu çalışmada, Bozcaada’yı ziyaret eden turistler üzerinden turist duyguları, turist memnuniyeti, destinasyon aşkı ve destinasyon sadakati arasındaki yapısal ilişkiler incelenmiştir. Kolayda örnekleme yöntemi kullanılarak 21/02/2020-2/05/2020 tarihleri arasında 448 turistten online ortamda veri toplanılmıştır. Araştırmadaki yapıları ortaya çıkarmak için açıklayıcı ve doğrulayıcı faktör analizi uygulanmıştır. Araştırma hipotezleri yapısal eşitlik modellemesi ile test edilmiştir. Araştırma sonucunda turist duygularının turist memnuniyeti ve destinasyon aşkını olumlu etkilediği; destinasyon aşkının destinasyon sadakatini olumlu etkilediği ve turist duyguları, turist memnuniyeti ve destinasyon aşkının destinasyon sadakatini olumlu etkilediği tespit edilmiştir. Elde edilen sonuçlar doğrultusunda Bozcaada için bazı öneriler sunulmuştur.
EXAMINING THE DIVERSE CONTRIBUTIONS OF EL TO SYSTEMIC LIPID METABOLISM

English version . 2 Abstract Versione in Lingua Italiana . 4 Introduction . 10 1 Plasma Lipases: at the crossroads between lipoprotein and tissue lipid metabolism . 11 1.1 Overview of Plasma Lipoprotein Metabolism . 11 Plasma lipoprotein classification . 12 Table I-1 Classification of the major human plasma lipoproteins . 12 Plasma lipoprotein composition . 13 Chylomicrons . 13 Very-Low Density Lipoproteins . 13 Intermediate-Density lipoproteins . 14 Low Density Lipoproteins . 14 High Density Lipoproteins . 14 Table I-2 Lipid and apolipoprotein composition of major plasma lipoproteins . 15 Pathways of plasma lipoprotein metabolism . 15 The exogenous pathway . 15 The endogenous pathway . 16 Reverse Cholesterol transport . 17 Figure I-1 Schematic of plasma lipoprotein metabolism . 19 1.2 Tissue Fatty acid metabolism . 20 Liver . 20 Mechanisms of FA uptake . 21 De novo Lipogenesis . 22 Regulation of De novo Lipogenesis . 23 Figure I-2 Regulation of de novo lipogenesis in the liver by ChREBP, SREBP1c and LXR. 26 Adipose Tissue . 27 Skeletal muscle . 28 2 Structure, function and physiology of the plasma lipase family members . 29 Figure I-3: Phylogenetic relationships of members of the pancreatic lipase gene family. 30 2.1 Structure . 30 Lipoprotein Lipase. 30 Hepatic Lipase . 31 Endothelial Lipase . 32 Table I-3 Summary of LPL, LIPC and LIPG gene characteristics . 33 Figure I-4. LPL, HL and EL gene and protein structure. 34 2.2 Post-transcriptional modifications . 35 2.3 Tissue expression . 36 Lipoprotein Lipase. 36 Hepatic Lipase . 36 Endothelial Lipase . 37 2.4 Activity, physiology and regulation of plasma lipases . 38 Lipoprotein Lipase. 38 Lipolytic activity . 38 Figure I5. The lipase-mediated TG hydrolysis reaction. 39 Non-lipolytic activity . 40
Identification and functional characterization of an ABC transporter of Haemonchus contortus, the P-glycoprotein 13

Les lactones macrocycliques (LM) sont des anthelminthiques (AH) a effet paralysant tres utilises chez les animaux et les humains contre les nematodes parasites. Cependant, leur succes therapeutique est compromis par la resistance croissante aux LM, qui pourrait etre en partie du aux ABC transporteurs P-glycoproteines (Pgps) selectionnes et surexprimes chez les nematodes resistants aux LM. Dans ce travail, nous avons etudie plus precisement la P-glycoproteine 13 du parasite de petits ruminants, Haemonchus contortus. Son orthologue chez le modele nematode C. elegans, Cel-Pgp-13, est exprime dans les amphides, structures qui ont ete associees a la sensibilite aux AH chez C. elegans et H. contortus. Pour predire la capacite des Pgps de nematode a transporter des drogues, incluant des LM et autres AH, nous avons developpe un modele de docking in silico. Nous avons utilise la structure cristallographique de C. elegans Pgp-1 (Cel-Pgp-1), et nous avons montre la liaison avec une forte affinite de plusieurs ligands decrits comme activateurs de sa fonction ATPasique. Nous avons aussi decrit une forte affinite des LM, et un site specifique de liaison de ces composes a Cel-Pgp-1. Cette approche represente un outil important pour predire les interactions entre AH, et pour concevoir rationnellement de nouveaux inhibiteurs competitifs des Pgps de nematode, dans le but d'ameliorer les strategies therapeutiques. Sur la base de cette approche, nous avons predit la structure 3D de Hco-Pgp-13 a partir du cristal de Cel-Pgp-1 afin d'etudier son interaction avec des substrats potentiels, en particulier les LM. Nous avons trouve des affinites similaires pour differents composes precedemment testes sur Cel-Pgp-1. In vitro, la mesure de l'activite ATPasique montre que l'actinomycine D est un substrat de Hco-Pgp-13. Nos donnees demontrent la presence possible d'un domaine de reconnaissance multispecifique sur ce transporteur de parasite. La determination par immunofluorescence de l'expression de Hco-Pgp-13 a montre une distribution tissulaire large indiquant que Hco-Pgp-13 pourrait jouer un role important dans le transport de substrats endogenes et/ou exogenes. En conclusion, ce travail permet de mieux comprendre le role des Pgps de nematodes dans le transport de medicaments AH, tant au niveau de l'organisme modele C. elegans que du nematode parasite H. contortus. Cette etude suggere la conservation de la fonction de tranporteur ABC multidrogue dans ces especes. La localisation de Hco-Pgp-13 sur les structures amphidiales, et son eventuelle implication dans la resistance aux medicaments et a la survie de H. contortus a l'exposition a des composes AH, restent a preciser.
Characterization of a gene regulating antibiotic production in Bacillus subtilis BSD-2

ABSTRACT Bacillus subtilis BSD-2 produces small molecule antibiotics that effectively control grey mould. To isolate the genes responsible for these antimicrobial activities, a B. subtilis BSD-2 mutant library was constructed utilizing the pHV1249 plasmid with a mini-Tn10 transposon. The antimicrobial potential of the generated strains was evaluated in the presence of cucumber grey mould and the mutant sequences were cloned by chromosome walking. Among the generated mutants, four mutants showed enhanced inhibitory effects in the presence of grey mould, and one mutant lost its inhibitory effect. The negative 1–19 mutant strains showed 98% sequence homology of the yodF gene, which encodes a Na+/metabolite permease. Next, we constructed a yodF knockout vector (pB-y-k) and used it to transform B. subtilis. A mutant yodF strain lost its inhibitory effect on grey mould, whereas reversion of the mutation restored the fungistatic activity.
The development of Self-Directed Learning skills of Teacher Candidates through Community-Based Recreation Program

The purposes of this experimental study with pretest-posttest control-group design were to examine the effect of community-based recreation program (CBRP) in developing of self-directed learning skills (SDLS) among teacher candidates and to evaluate their satisfaction of CBRP. The samples were 60 teacher candidates from Srinakharinwirot University. They were divided equally into two groups (experimental and control group) by using multi-stage sampling. Research instruments were: a CBRP for SDLS development of teacher candidates, which face validity was approved by five experts; and two questionnaires designed by researcher to evaluate teacher candidates’ satisfaction of CBRP and assess their SDLS. Both questionnaires using IOC = 0.8-1.0 on all item with five experts also approved the content validity. Cronbach’s Alpha on all item were 0.80 or higher respectively for coefficient of reliability. Data was analysed by means, S.D. , and dependent t-test, independent t-test, and effect size (Cohen’s d). The results indicated that: 1) after the CBRP was completed, the experimental group scored better in both overall and each item of SDLS than before joining the program with significant differences of 0.05, 2) there was no significant difference in the overall and each item of SDLS in the control group between before and after the CBRP was completed; 3) after the CBRP was completed, the experimental group SDLS’s scores were better in both overall and each item than control group at the significant differences of 0.05. Finally, the effect size (Cohen’s d) of this study indicated that the experimental group is large enough to have an influence on the population (d = 18.52); 4) the experimental group scored the highest on the satisfaction evaluation for the CBRP.
Monitoring with microchips: Microchip‐automated doors as a potential novel method for tracking the survival of released Northern Brown Bandicoots

Monitoring animals after release to the wild is often limited due to financial and logistical constraints. Microchip-automated technology has the potential to provide data on microchipped animals' survival after release and allows access to only the intended, microchipped animals. We aimed to determine whether Northern Brown Bandicoot (Isoodon macrourus) trained to use microchip-automated nest boxes in captivity would continue to use them after release back to the wild. Ten animals were brought into captivity and trained to use the nest boxes in as little as three days and then released back to the wild after approximately three months in captivity. Use of the nest boxes after release was recorded for up to a week. Eight of the ten animals used the nest boxes on the first night, but only two used them more than five days after release. Three of the animals did not use them at all after release. Whilst ongoing utilisation of the nest boxes was generally low, this technology shows great potential for other species that are hollow-dependent.
Is the investment decision affected by the independence and competence of the audit committee? A comparative study between Bahrain and Indonesia

Purpose This study aims to investigate the impact of the audit committee’s (AC’s) independence and competence in the company’s investment decision-making in Bahraini- and Indonesian-listed firms, then to compare the two results Design/methodology/approach A quantitative method is used and cross-sectional data are collected through a self-administered questionnaire survey. A stratified random sample technique is adopted with a total of 409 respondents from 39 listed companies in Bahrain and 303 respondents from 27 companies listed on the Indonesia Stock Exchange (IDX). A descriptive analysis is used to identify the characteristics of the respondents, while a correlation analysis, linear regression and t-test analyses are used to test the model, explain the relationships among variables and compare the two studies (Bahrain vs Indonesia). Findings It is found that the AC independence and AC competence have a positive and significant influence on investment decision-making for both the Bahrain and the Indonesia studies Practical implications The current study’s results have implications for the process of appointing and nominating the AC members, since this would affect an investor’s investment decision. Investors’ perception of the independence and competence of ACs will make a difference in their investment decisions. Originality/value AC independence and competence are importantly crucial for the decision-makers in improving the quality of financial reporting, internal control, and audit. This may lead to an increase in investors’ trust in financial reports and their ability to make favorable investment decisions.
Deep CNN, Body Pose, and Body-Object Interaction Features for Drivers’ Activity Monitoring

Automatic recognition and prediction of in-vehicle human activities has a significant impact on the next generation of driver assistance and intelligent autonomous vehicles. In this article, we present a novel single image driver action recognition algorithm inspired by human perception that often focuses selectively on parts of the images to acquire information at specific places which are distinct to a given task. Unlike existing approaches, we argue that human activity is a combination of pose and semantic contextual cues. In detail, we model this by considering the configuration of body joints, their interaction with objects being represented as a pairwise relation to capture the structural information. Our body-pose and body-object interaction representation is built to be semantically rich and meaningful, which is highly discriminative even though it is coupled with a basic linear SVM classifier. We also propose a Multi-stream Deep Fusion Network (MDFN) for combining high-level semantics with CNN features. Our experimental results demonstrate that the proposed approach significantly improves the drivers’ action recognition accuracy on two exacting datasets.
Citizen Science in the Field: Co-experimentation at Pilot Scale for Sustainable Use of Natural Resources

Natural resource management is a cross-sectoral topic, as reflected by its inclusion in several of the United Nations Sustainable Development Goals (e.g., SDGs 2, 6, 12, 15). In the study area on Bangka Island, Indonesia, agriculture is a pillar of local food security and livelihoods, making restoration of degraded lands also a local issue. The present study aims at restoring degraded land after tin mining and at restoring the natural soil base rendering it more suitable for agriculture. We use co-experimentation with citizens as a tool to develop options for re-habilitation at a pilot scale. The recruitment process in this study was reversed insofar as local citizens were at the origin of the project idea. Consequently, buy-in was high among local stakeholders. This set to increase the probability of successfully scaling up effective and actionable practices that were developed during co-experimentation at both local and regional levels. Co-experimentation provided a platform for exchange between local citizens and scientists. Citizens did not need to learn new skills to be able to participate in the scientific process and could autonomously evaluate results. We see involvement of citizens in this type of scientific projects not only as feasible, but as rewarding for all involved partners and as beneficial for the project outcomes. In light of the call for partnerships to achieve the Sustainable Development Goals, we can only recommend investing in communication and relationship building to work together on better solutions to the challenges we face.
Primeiro registro da ocorrência de protozoários em Tamoatá hoplosternum littorale no Brasil

This study reports the first occurrence of protozoans in gills of catfish Hoplosternum littorale (Hancock 1828) (Siluriformes: Callichthyidae) from Brazil. From the examined specimens during period of July to August 2011, 95.3% had the gills infected only by protozons Ichthyophthirius multifiliis (Ciliophora) and/or Piscinoodinium pillulare (Dinoflagellida), being but the highest infection levels were caused by I. multifiliis. The abundance of these parasites was not influenced by the fish body size and also not affected the relative condition factor (Kn) of the hosts. This was the first report of epidemiologic indices for H. littorale in the Brazilian Amazon.
A review on health benefits of kombucha nutritional compounds and metabolites

ABSTRACT Kombucha is a beverage made by fermenting sugared tea using a symbiotic culture of bacteria and yeasts. Kombucha consumption has been associated with some health effects such as: the reduction of cholesterol levels and blood pressure, reduction of cancer propagation, the improvement of liver, the immune system, and gastrointestinal functions. The beneficial effects of kombucha are attributed to the presence of bioactive compounds that act synergistically. Bacteria contained in kombucha beverage belongs to the genus Acetobacter, Gluconobacter, and the yeasts of the genus Saccharomyces along with glucuronic acid, contribute to health protection. This review focuses on recent findings regarding beneficial effects of kombucha and discusses its chemical compounds, as well as the metabolites resulted by the fermentation process. Besides, some contraindications of kombucha consumption are also reviewed.
Studying the efficacy of fipronil (GR 0.2%) against European Mole Cricket, Gryllotalpa gryllotalpa (Orthoptera: Gryllotalpidae)

The European Mole Cricket, Gryllotalpa gryllotalpa (Orthoptera: Gryllotalpidae), is a key pest of several crops in different regions of the world, damaging seedlings, roots and tubers. The efficacy of fipronil (Regent ® GR 0.2%) @ 2, 2.5 and 3 g/m2 along with toxic bait of carbaryl (EC 85%)@ 20 g/m2 were assessed against this pest in the field based on a completely randomized block design. Based on Henderson-Tilton formula, on the third day after treatment, the efficacy of fipronil @ 2, 2.5 and 3 g/m2 and carbaryl bait was 16%, 30%, 47% and 53%, respectively; while on the third day after treatment, the efficacy was 22%, 48%, 64% and 81%, respectively. At present, carbaryl application is banned in Iran; therefore, fipronil can be a suitable substitute for this insecticide.
Rho1p mutations specific for regulation of β(1→3)glucan synthesis and the order of assembly of the yeast cell wall

In the yeast Saccharomyces cerevisiae, the GTP‐binding protein Rho1 is required for β(1→3)glucan synthase activity, for activation of protein kinase C and the cell integrity pathway and for progression in G1, cell polarization and exocytosis. A genetic screen for cells that become permeabilized at non‐permissive temperature was used to isolate in vitro‐generated mutants of Rho1p. After undergoing a battery of tests, several of them appeared to be specifically defective in the β(1→3)glucan synthesis function of Rho1p. At the non‐permissive temperature (37°C), the mutants developed defects in the cell wall, especially at the tip of new buds. In the yeast cell wall, β(1→6)glucan is linked to both β(1→3)glucan and mannoprotein, as well as occasionally to chitin. We have used the rho1 mutants to study the order of assembly of the cell wall components. The incorporation of [14C]‐glucose into β(1→3)glucan at 37°C was decreased or abolished in the mutants. Concomitantly, a partial defect in the incorporation of label into cell wall mannoproteins and β(1→6)glucan was observed. In contrast, YW3458, an inhibitor of glycosylphosphatidylinositol anchor formation, prevented mannoprotein incorporation, whereas the β(1→3)–β(1→6)glucan complex was synthesized at almost normal levels. As β(1→3)glucan can be synthesized in vitro or in vivo independently, we conclude that the order of addition in vivo is β(1→3)glucan, β(1→6)glucan, mannoprotein. Previous observations indicate that chitin is the last component to be incorporated into the complex.
An immunohistochemical study of histone H1.5 in correlation with clinicopathologic features of invasive duct carcinoma (not otherwise specified)

Background Breast cancer is a leading cause of malignant mortality and morbidity in women worldwide. Histones are a family of linker proteins that are located in the nucleus. Histone H1.5 (HH1.5) is one of the families involved in the stabilization of chromatin structure and gene regulation. It shows altered expression and mutation in different types of human cancers. Objective This study aimed to evaluate immunohistochemical expression of HH1.5 in invasive duct carcinoma in relation to pathologic parameters including histologic tumor grade, pathologic staging, and molecular subtyping. Materials and methods A retrospective study was carried out on 60 samples of invasive duct carcinoma, not otherwise specified (NOS). Immunohistochemical staining with the HH1.5 antibody was performed. Results HH1.5-positive expression was observed in 70% of the cases (42 of 60 cases). There was a statistically significant correlation between increased HH1.5-positive immunohistochemical expression and each of increased tumor size, increased lymph node positivity, and tumor histologic grade. There was a statistically significant association between the presence of lymphovascular invasion and a high HH1.5 score. Among estrogen receptor, progesterone receptor, and HER2/neu scores, only the Ki67 labeling index showed a significant positive correlation with the HH1.5 score. There was a highly significant difference between cases with different HH1.5 scores in terms of molecular types. Conclusion The high expression of HH1.5 was associated with poor prognostic factors, including its differential expression in the carcinoma molecular types. Further characterization of the role of HH1.5 in breast carcinomas should be performed for the development of novel targeted therapies in this type of cancer.