Miracle-dz/UniFAR-FTUs huggingface dataset:
Negative Third-Party Reactions to Male and Female Victims of Rape: The Influence of Harm and Normativity Concerns

Male and female victims of sexual violence frequently experience secondary victimization in the form of victim blame and other negative reactions by their social surroundings. However, it remains unclear whether these negative reactions differ from each other, and what mechanisms underlie negative reactions toward victims. In one laboratory study (N = 132) and one online study (N = 421), the authors assessed participants’ reactions to male and female victims, and whether different (moral) concerns underlay these reactions. The reactions addressed included positive and negative emotions, behavioral and characterological blame, explicit and implicit derogation, and two measures of distancing. It was hypothesized that male victimization would evoke different types of (negative) reactions compared with female victimization, and that normative concerns would predict a greater proportion of the variance of reactions to male victims than female victims. Multivariate analyses of variance (MANOVAs) were conducted to test whether reactions to male and female (non-)victims differed. Multiple regression analyses were conducted to test the influence of gender traditionality, homonegativity, as well as binding and individualizing moral values on participants’ reactions. Results revealed that participants consistently reacted more negatively to victims than to nonvictims, and more so to male than to female targets. Binding values were a regular predictor of negative reactions to victims, whereas they predicted positive reactions to nonvictims. The hypothesis that different mechanisms underlie reactions to male versus female victims was not supported. The discussion addresses implications of this research for interventions targeting secondary victimization and for future research investigating social reactions to victims of sexual violence. It also addresses limitations of the current research and considerations of diversity.
Syllable duration changes during babbling: a longitudinal study of French infant productions

Abstract At the babbling stage, the syllable does not have the temporal characteristics of adult syllables because of the infant's limited oro-motor skills. This research aims to further our knowledge of syllable duration and temporal variability and their evolution with age as an indicator of the development of articulatory skills. The possible impact of syllable position, as well as that of type of intrasyllabic associations and intersyllabic articulatory changes on these parameters has also been tested. Oral productions of 22 French infants were recorded monthly from 8 to 14 months. 11 261 Consonant-Vowel (CV) syllables were annotated and temporally analyzed. The mean duration varied according to syllable position, but not to the intrasyllabic or intersyllabic articulatory changes. Moreover, the syllable duration decreased significantly from the age of 10 months onwards, whereas the temporal variability remained the same.
Exposure to Traffic-Related Particulate Matter 2.5 Triggers Th2-Dominant Ocular Immune Response in a Murine Model

Ambient particulate matter (PM), a major component of air pollution, aggravates ocular discomfort and inflammation, similarly to dry eye disease (DED) or allergies. However, the mechanism(s) by which PM induces the ocular inflammatory response is unknown. This study investigated the immunological response of traffic-related fine particulate matter (PM2.5) on the ocular surface in a murine model. C57BL/6 mice were exposed by topical application to PM2.5 or vehicle for 14 days to induce experimental environmental ocular disease. Corneal fluorescein staining and the number of ocular inflammatory cells were assessed in both groups. The expression of IL-1β, IL-6, tumor necrosis factor (TNF)-α, and mucin 5AC (MUC5AC) in the ocular surface were evaluated by real-time PCR. An immunohistochemical assay evaluated apoptosis and goblet cell density. ELISA was used to determine the levels of serum IgE and cytokines of Type 1 helper (Th1) and Type 2 helper (Th2) cells after in vitro stimulation of T cells in the draining lymph nodes (LNs). Exposure to traffic-related PM2.5 significantly increased corneal fluorescein staining and cellular toxicity in the corneal epithelium compared with the vehicle control. A significant increase in the number of CD11b+ cells on the central cornea and mast cells in the conjunctiva was observed in the PM2.5 group. Exposure to PM2.5 was associated with a significant increase in the corneal or conjunctival expression of IL-1β, IL-6, TNF, and MUC5AC compared to the vehicle, and increased maturation of dendric cells (DCs) (MHC-IIhighCD11c+) in draining LNs. In addition, PM2.5 exposure increased the level of serum IgE and Th2 cytokine production in draining LNs on day 14. In conclusion, exposure to traffic-related PM2.5 caused ocular surface damage and inflammation, which induced DC maturation and the Th2-cell-dominant allergic immune response in draining LNs.
Inhibition of Band 3 tyrosine phosphorylation: a new mechanism for treatment of sickle cell disease

Many hypotheses have been proposed to explain how a glutamate to valine substitution in sickle haemoglobin (HbS) can cause sickle cell disease (SCD). We propose and document a new mechanism in which elevated tyrosine phosphorylation of Band 3 initiates sequelae that cause vaso‐occlusion and the symptoms of SCD. In this mechanism, denaturation of HbS and release of heme generate intracellular oxidants which cause inhibition of erythrocyte tyrosine phosphatases, thus permitting constitutive tyrosine phosphorylation of Band 3. This phosphorylation in turn induces dissociation of the spectrin‐actin cytoskeleton from the membrane, leading to membrane weakening, discharge of membrane‐derived microparticles (which initiate the coagulation cascade) and release of cell‐free HbS (which consumes nitric oxide) and activates the endothelium to express adhesion receptors). These processes promote vaso‐occlusive events which cause SCD. We further show that inhibitors of Syk tyrosine kinase block Band 3 tyrosine phosphorylation, prevent release of cell‐free Hb, inhibit discharge of membrane‐derived microparticles, increase sickle cell deformability, reduce sickle cell adhesion to human endothelial cells, and enhance sickle cell flow through microcapillaries. In view of reports that imatinib (a Syk inhibitor) successfully treats symptoms of sickle cell disease, we suggest that Syk tyrosine kinase inhibitors warrant repurposing as potential treatments for SCD.
Sub physiological temperature induces pervasive alternative splicing in Chinese hamster ovary cells

RNA sequencing (RNASeq) has been widely used to associate alterations in Chinese hamster ovary (CHO) cell gene expression with bioprocess phenotypes, however alternative mRNA splicing, has thus far, received little attention. In this study, we utilised RNASeq for transcriptomic analysis of a monoclonal antibody producing CHOK1 cell line subjected to a temperature shift. More than 2,365 instances of differential splicing were observed 24hrs after the reduction of cell culture temperature. 1,163 of these alternative splicing events were identified in genes where no changes in abundance were detected by standard differential expression analysis. Ten examples of alternative splicing were selected for independent validation using qPCR in the monoclonal antibody producing CHOK1 line used for RNASeq and a further 2 CHOK1 cell lines. This analysis provided evidence that exon skipping and mutually exclusive splicing events occur in genes linked to the cellular response to changes in temperature and mitochondrial function. While further work is required to determine the impact of changes in mRNA sequence on cellular phenotype, this study demonstrates that alternative splicing analysis can be utilised to gain a deeper understanding of post-transcriptional regulation in CHO cells during biopharmaceutical production.
Biomarkers Predicting for Response and Relapse with Melanoma Systemic Therapy

Introduction of new systemic therapies in the last 10 years has radically improved outcomes for melanoma patients. Even so, not all patients benefit, so getting the right treatment to the right patient is a priority. These two major drug classes, small molecule targeted kinase inhibitors and immune check-point inhibitors, both come at significant cost, with sometimes serious side effects as well as high expense for health services. Almost half of melanomas harbour a >BRAF>V600 mutation and virtually all patients receiving BRAF targeted therapy will experience some amount of response. However, duration of response with these agents is uncertain, due to acquired resistance, which means few patients remain in response long term. Most metastatic melanoma patients are potentially eligible for immune checkpoint inhibitors, irrespective of BRAF status. However, only about half of patients will respond to these agents, and only half again will benefit long term. Thus, both primary and acquired resistance limit response. In this era of personalized anti-cancer therapy, biomarkers offer a means to predict for both response and relapse to a particular treatment. To date, the only validated bio-marker applied to selecting melanoma systemic therapy is the >BRAF gene. However, modern technologies are now opening up a wide range of candidate genes, polypeptides and proteins which are being evaluated for their potential clinical application as predictive biomarkers of the future.
Outcomes of incisional negative pressure wound therapy following brachiobasilic transposition arteriovenous fistula creation: A 1:2 propensity score matched study

Incisional negative wound pressure therapy (iNPWT) use on closed incisions has been shown to improve wound outcomes, but no studies have evaluated the use of iNPWT following brachiobasilic transposition arteriovenous fistula (BBT‐AVF). We aim to investigate the efficacy of iNPWT vs conventional wound therapy in reducing surgical site infections (SSIs) for BBT‐AVF incisions. This is a retrospective cohort study of patients who underwent BBT‐AVF creation between January 2010 and December 2017. A 1:2 propensity score matching (PSM) was performed to reduce selection bias and address for confounding factors. Study outcomes included SSI and haematoma incidence, 30‐day readmission, and 30‐day mortality. A total of 154 patients were reviewed in this study: 47 (30.5%) had iNPWT and 107 (69.5%) had conventional wound therapy. The overall median age was 60.5 (interquartile range 54–69). PSM with a 1:2 ratio resulted in a total of 117 patients (39 iNPWT and 78 conventional wound therapy). In the unmatched cohort, SSI incidence was lower in the iNPWT group (n = 1/47 [2.1%] vs n = 14/107 [13.1%], P = .035). However, incidence of SSI was comparable between iNPWT and conventional wound therapy after matching (n = 1/39 [2.6%] vs n = 9/78 [11.5%], P = .102). There was no significant difference in 30‐day readmission and 30‐day mortality. Within our study population of patients with BBT‐AVF incisions, there is a non‐statistically significant reduction in SSI incidence for patients who received iNPWT as compared with conventional wound therapy. Further prospective randomised controlled studies should be conducted to validate these findings.
Evaluación de caracteres de planta y fruto en líneas recombinantes autofecundadas de tomate obtenidas por cruzamiento entre Lycopersicon esculentum y L. pimpinellifolium

G.R. Rodriguez, G.R. Pratta, R. Zorzoli and L.A. Picardi. 2006. Evaluation of plant and fruit traits in recombinant inbred lines of tomato obtained from a cross between Lycopersicon esculentum and L. pimpinellifolium. Cien. Inv. Agr. 33(2):133-141. Seventeen recombinant inbred lines of tomato (Lycopersicon esculentum) were obtained from an interspecifi c cross between tomato cv. 'Caimanta' and the accession LA722 of L. pimpinellifolium. Plant and fruit traits were evaluated in these lines as well as in the parental genotypes that served as controls. Signifi cant differences were found among parental genotypes and recombinant inbred lines for plant traits (internodes length between third and fourth node, number of fl owers per infl orescence, stem perimeter at the basal, middle, and apical part) and fruit traits (soluble solid content, pH, acidity, diameter, height, shape, weight, and shelf life). Signifi cant differences were also found among recombinant inbred lines, in spite of the fact that many of them were similar to the wild parent. Fifteen lines had longer shelf life than LA722 that was the parent with the longest shelf life. A multivariate analysis of canonical correlation with plant and fruit traits and a cluster analysis were performed to classify the lines and the parental genotypes according to their performance for these traits. The fi rst canonical correlation explained 73% of the total variance (p<0.01). According to the canonical coeffi cients those plants with the biggest stem perimeter and the lowest number of fl owers per cluster produced the heaviest and the biggest fruits but they had low acidity. In the cluster analysis, shelf life was an important discriminatory trait for these lines and the parental genotypes. 'Caimanta' was the only genotype located in one group. The other clusters had genotypes with similar values for stem perimeter, fruit size, fruit weight, acidity and fruit shelf life. It was possible to obtain new genotypes that have recombinant traits from the parental genotypes and some of the new genotypes have an even longer shelf life than the wild parent. In conclusion, some of these new genotypes would be a new source of variability useful in tomato breeding programs.
Decanal Protects against UVB-Induced Photoaging in Human Dermal Fibroblasts via the cAMP Pathway

Solar ultraviolet (UV) radiation is the primary factor of cutaneous aging, resulting in coarse wrinkles and dryness. In this study, we aimed to test whether decanal, an aromatic compound found mainly in citrus fruits, inhibits UVB-mediated photoaging in human dermal fibroblasts and to explore whether its anti-photoaging effect occurs via cyclic adenosine monophosphate (cAMP) signaling. We found that decanal promotes collagen production dose-dependently. Meanwhile, it also increased the intracellular cAMP levels and decreased the number of molecules involved in the mitogen-activated protein kinase (MAPK)/activator protein 1 (AP-1) pathway, downregulating the collagen genes and upregulating the matrix metalloproteinase (MMP) genes in UVB-exposed dermal fibroblasts. Furthermore, it enhanced hyaluronic acid levels and hyaluronic acid synthase mRNA expression. Notably, the beneficial effects of decanal were lost in the presence of a cAMP inhibitor. Our results revealed the potential of decanal for preventing photoaging and suggested that its effects are cAMP-mediated in human dermal fibroblasts.
Primary brain cells in in vitro controlled microenvironments : single cell behaviors for collective functions

The complex structure of the brain is explored by various methods, such as neurophysiology and cognitive neuroscience. This exploration occurs at different scales, from the observation of this organ as a whole entity to molecules involved in biological processes. Here, we propose a study at the cellular scale that focuses on two building elements of brain: neurons and glial cells. Our approach reachs biophysics field for two main reasons: tools that are used and the physical approach to the issues. The originality of our work is to keep close to the in vivo by using primary brain cells in in vitro systems, where chemical and physical environments are controled at micrometric scale. Microelectronic tools are employed to provide a reliable control of the physical and chemical cellular environment. This work focuses on two aspects of brain cell biology: neuronal polarization and glial cell sensitivity to mechanical properties of their environment. As an example, these two issues are involved in injured brains. The first is crucial for the directionality of the transmission of electrical and chemical signals and is associated to a break of symmetry in neuron morphology. The second occurs in recolonization mechanisms of lesions, whose mechanical properties are impaired. During this thesis, quantitative studies are performed on these two cell types, focusing on their growth and their response to geometrical and mechanical constraints. The final aim is to elucidate some molecular mechanisms underlying changes of the cellular structure, and therefore of the cytoskeleton. A significant outcome of this work is the control of the neuronal polarization by a simple control of cell morphology. This result opens the possibility to develop controlled neural architectures in vitro with a single cell precision.
Reproductive Traits of an Invasive Alien Population of Grey Squirrel (Sciurus carolinensis) in Central Italy

Simple Summary The presence of an invasive alien species is one of the main causes of biodiversity loss, which occurs through the reduction or even the extinction of native populations. In this context, the EU 2020 biodiversity strategy calls for research on invasive alien species in order to prevent their introduction and spread more effectively. The grey squirrel (Sciurus carolinensis), which is native to North America, provides one of the best-known examples of competitive exclusion of a native one, the European red squirrel (Sciurus vulgaris). A grey squirrel population, living in central Italy (Umbria), was studied to provide parameters on its population dynamics, growth rates, and viability, in order to understand its adaptation to its non-native range. Our data revealed that the Umbrian grey squirrel population is well-adapted to its new range, undergoing two annual mating periods and experiencing high reproductive success. Therefore, these invasive grey squirrels pose a substantial threat to the local European red squirrel population. Abstract The reproductive cycle of an invasive alien Italian grey squirrel population was studied to understand its adaptation and limit its spread, in order to conserve the autochthonous red squirrel. Female and male genital traits were evaluated throughout the reproductive cycle, including the ovary, uterus, testicle, epididymis, seminiferous tubule morphometry, and germinative epithelium histology. Moreover, individual female fecundity was determined by counting uterine scars. Ovary width and uterus weight, length, and width reached their highest values in the luteal and pregnancy phases. On conducting a histological evaluation of the testicular germinal epithelium, four morphotypes related to the different reproductive phases of the male squirrels were identified: immature, pubertal, spermatogenesis, and regressive. Testicle and epididymis weights and seminiferous tubule diameters reached their largest values during spermatogenesis. Uterine scar analysis showed that 69% of the females had given birth to one or two litters, while 31% had no uterine scars. Litters were larger in the first breeding period than in the second; annual fecundity was 4.52 ± 1.88 uterine scars/female. Umbrian grey squirrels have adapted to their non-native range, showing two annual mating periods at times similar to those in their native range, and high reproductive success.
Higher Depressive Symptoms Predict Lower Social Adaptive Functioning in Children and Adolescents with ASD

ABSTRACT Objective: Despite the frequent occurrence of depressive symptoms in children and adolescents with autism spectrum disorder (ASD), few studies have investigated the relationship between depressive symptoms and adaptive functioning. The present study explored the impact of depressive symptoms on different domains of adaptive functioning in children and adolescents with ASD. Methods: Depressive symptoms and adaptive functioning were analyzed in 62 children and adolescents with ASD (20 females) and 36 children and adolescents (15 females) with typical development between 5 and 18 years of age. Results: After controlling for IQ, age and sex, higher depressive symptoms predicted lower functioning in the social domain among children and adolescents with ASD. Depressive symptoms did not significantly predict communication or daily living skills. Conclusions: These findings highlight the relevance of depression in social adaptive function in ASD and emphasize the importance of assessing depressive symptomatology when evaluating social skills and planning treatment for children and adolescents with ASD.
Potential pollinators and robbers: a study of the floral visitors of Heliconia angusta (Heliconiaceae) and their behaviour

Floral syndromes are traditionally thought to be associated with particular pollinator groups. Ornithophilous flowers tend to have traits that facilitate bird pollination such as having long, narrow, tubular corollas, often vivid coloration and diluted, sucrose-rich nectar. However, recent studies have shown that flowers attract a broader spectrum of visitors than might be expected. Furthermore, the classification of floral visitors as ‘robbers’ or ‘pollinators’ often is not as simple as it seems, as pollinators can at times act as robbers and vice versa. We studied the species composition, behaviour and ecology of floral visitors, including potential pollinators and robbers, of Heliconia angusta (Heliconiaceae), an endemic understory herb of the Atlantic Rainforest of Brazil. In addition, the impact of the plant inflorescence attractiveness and of weather and light conditions on visitor abundance and frequency was investigated. Flower visitors were found to be scarce with a total of only 151 visits being observed during 120 h of field observations. A stingless bee species (Trigona sp.) appeared to be the most abundant visitor to the ornithophilous flowers of H. angusta , along with four different species of hummingbirds and two species of butterflies. We consider Trigona sp. rather as pollen robber, but which still has the potential to be a secondary pollinator, whereas the hummingbirds were the principle legitimate visitors. Most flower visitors were recorded between 9.00 am and 1.00 pm with a higher number visiting under semi-shaded conditions than in full shade. Hummingbird numbers increased with flower abundance while the other visitor group numbers were not affected.
Heavy Eye Syndrome Mimicking Abducens Nerve Palsies

A 70-year-old woman was seen in neuro-ophthalmology consultation for a progressive right esotropia. She had a past medical history of asthma and osteoporosis, and her ocular history was significant for pathological myopia (spherical equivalent of −23.00 D OD and −21.00 D OS), bilateral cataract surgeries, and laser retinopexy for retinal tears. Twenty-five years prior to presentation, she developed binocular horizontal diplopia and was found to have a limitation of abduction to 60% and 80% of normal in the right and left eyes, respectively, by a previous neuro-ophthalmologist. She developed a progressive maculopathy in the right eye secondary to myopia, resulting in the resolution of double vision. The resolution occurred because the double vision was binocular in nature and the loss of vision in her right eye functioned similar to monocular occlusion. However, she noticed that her right eye continued to turn in toward her nose.