Miracle-dz/UniFAR-FTUs huggingface dataset:
Purification of the endogenous glucocorticoid receptor stabilizing factor.

A ubiquitous, low molecular weight, heat-stable component of cytosol stabilizes the glucocorticoid receptor in its untransformed state in association with hsp90. This heat-stable factor mimics molybdate in its effects on receptor function, and it has the heat stability, charge, and chelation properties of a metal oxyanion [Meshinchi, S. , Grippo, J.F. , Sanchez, E.R. , Bresnick, E.H. , & Pratt, W.B. (1988) J. Biol. Chem. 263, 16809-16817]. In this paper, we describe the further purification of the endogenous factor from rat liver cytosol by anion-exchange HPLC (Ion-110) after prepurification by molecular sieving, cation absorption, and charcoal absorption. Elution of the factor with an isocratic gradient of ammonium bicarbonate results in recovery of all of the bioactivity in a single peak which coelutes with inorganic phosphate and contains all of the endogenous molybdenum. The bioactivity can be separated from inorganic phosphate by chromatography of the partially purified endogenous factor on a metal-chelating column of Chelex-100. The chelating procedure results in complete loss of bioactivity with recovery of 98% of the inorganic phosphate in both the column drop-through and a subsequent 1 M NaCl wash. The factor preparation purified through the Ion-110 HPLC step inhibits temperature-mediated dissociation of the immunopurified glucocorticoid receptor-hsp90 complex, but it is considerably more effective at stabilizing the unpurified receptor-hsp90 complex in a Chelex-treated cytosol system that has been depleted of metal components. These observations support the proposal that an endogenous metal can stabilize the binding of hsp90 to the receptor but it is likely that other cytosolic components that are not present in the immunopurified complex must contribute to the stability of the soluble protein-protein complex in cytosol.
The WRKY57 Transcription Factor Affects the Expression of Jasmonate ZIM-Domain Genes Transcriptionally to Compromise Botrytis cinerea Resistance1

The negative role of AtWRKY57 in Botrytis infection results from transcriptional competition between transcription factors WRKY57 and WRKY33 and their actions on downstream target JAZ1 and JAZ5 genes. Although necrotrophic pathogens cause many devastating plant diseases, our understanding of the plant defense response to them is limited. Here, we found that loss of function of WRKY57 enhanced the resistance of Arabidopsis (Arabidopsis thaliana) against Botrytis cinerea infection. Further investigation suggested that the negative regulation of WRKY57 against B. cinerea depends on the jasmonic acid (JA) signaling pathway. Chromatin immunoprecipitation experiments revealed that WRKY57 directly binds to the promoters of JASMONATE ZIM-DOMAIN1 (JAZ1) and JAZ5, encoding two important repressors of the JA signaling pathway, and activates their transcription. In vivo and in vitro experiments demonstrated that WRKY57 interacts with nuclear-encoded SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2. Further experiments display that the same domain, the VQ motif, of SIB1 and SIB2 interact with WRKY33 and WRKY57. Moreover, transient transcriptional activity assays confirmed that WRKY57 and WRKY33 competitively regulate JAZ1 and JAZ5, SIB1 and SIB2 further enhance these competitions of WRKY57 to WRKY33. Therefore, coordinated regulation of Arabidopsis against B. cinerea by transcription activators and repressors would benefit plants by allowing fine regulation of defense.
Prevalence and correlates of complementary and alternative medicine services use in low-income African Americans and whites: a report from the Southern Community Cohort Study.

OBJECTIVES This study aimed to examine the prevalence, trends, and correlates of practitioner-based complementary and alternative medicine (CAM) services use according to race in a socioeconomically disadvantaged population. DESIGN Included in this cross-sectional analysis were 50,176 African Americans (AAs) and 19,038 whites enrolled into the Southern Community Cohort Study from March 2002 through September 2009. Logistic regression models were used to calculate odds ratios (ORs) and 95% confidence intervals (CIs) of CAM services use associated with participant characteristics. OUTCOME MEASURES Outcomes include the prevalence of and trends in use of CAM services during 2002-2009 and correlates of use by race. RESULTS CAM services use during 2002-2009 was greater among whites (11.7%) than among AAs (8.5%), but no significant temporal trends within the 8-year period were observed. The significant associations were observed for CAM services use with higher educational attainment (OR 1.78, 95% CI: 1.61-1.96 for college versus less than high school), household income (OR 1.61, 95% CI: 1.44-1.81 for ≥$50,000 versus <$15,000), and having a history of a chronic disease (OR 1.34, 95% CI: 1.21-1.47) among both AAs and whites. Significant differences in findings between AAs and whites were seen for age (with a sharp decline in use with older age among AAs but not whites), sex (with the excess of female users more striking among whites), employment (with the unemployed among AAs but not whites more likely to be users), alcohol consumption (with white but not AA drinkers more likely to report CAM services use), and cigarette smoking status (with negative association of use with current smokers more striking among whites). CONCLUSIONS CAM services use is associated with sociodemographic and health-related factors, and racial differences in such use exist. The descriptive findings of this study help supplement the limited information on CAM use among low-income and minority populations in the United States.
Training Googles SyntaxNet to understand Norwegian Bokmål and Nynorsk (short paper)

We use Google’s open source neural network framework, SyntaxNet, to train a fully automatic part-of-speech language tagger for Norwegian Bokmal and Nynorsk. Using SyntaxNet, we are able to get comparable results with other tagger when tagging Bokmal and Nynorsk with part-of-speech. Both taggers are available and released as open source.
Commande de systèmes linéaires sous contraintes fréquentielles et temporelles : Application au lanceur flexible

Dans la plupart des problemes de synthese, la loi de commande obtenue doit repondre simultanement a des criteres requentiels et temporels en vue de satisfaire un cahier des charges precis. Les derniers developpements des techniques de synthese Hinf de controleurs structures permettent d'obtenir des lois de commande satisfaisant des criteres frequentiels multiples. En revanche, la synthese de loi de commande satisfaisant une contrainte temporelle sur une sortie ou un etat du systeme est plus complexe. Dans ce travail de these, la technique OIST est consideree pour ce type de contraintes. Elle consiste a saturer la sortie du controleur des que la contrainte n'est plus verifiee afin de restreindre l'ensemble des sorties admissibles. Initialement formulee pour les systemes lineaires connus dont l'etat est mesure, la technique OIST peut etre generalisee pour permettre de considerer des systemes incertains. C'est l'extension OISTeR qui est proposee dans ce travail. Elle utilise les donnees d'un observateur par intervalles pour borner de maniere garantie le vecteur d'etat. La theorie des observateurs par intervalles a recemment fait l'objet de nombreux travaux. La methode la plus rapide pour obtenir un observateur par intervalles d'un systeme donne est de considerer un systeme intermediaire cooperatif dans de nouvelles coordonnees. Une nouvelle technique de determination de ces nouvelles coordonnees, intitulee SCorplO, est proposee dans ce memoire. L'ensemble des techniques presentees est applique au controle d'un lanceur flexible durant son vol atmospherique, en presence de rafales de vent et sous contrainte temporelle sur l'angle d'incidence.
Linking IT and Business Processes for Alignment - A Meta Model based Approach

Methods to optimize alignment between an enterprises business strategy and its IT strategy has been on the agenda of IS research since the beginning of last decade. Recognizing the growing impact of IT on the revenue side and on the cost side of P&L, it has become one of the most pressing issues of strategic IT management since then. One promising approach in gaining best results for alignment is to synchronize similar and related processes in both the business and the IT domain. In this contribution we present an approach for identifying components of processes in both domains on the basis of existing meta models. We consider this as a first step in developing a method based coherent view on both domains which finally will allow us to create a systematic and comprehensive alignment method.
Retinal detachments due to juxtapapillary microholes.

The characteristic features of an unusual apparently rare clinical entity represented by juxtapapillary retinal microholes, the accompanying retinal detachments, occurring mostly in high myopic eyes, are described in 6 cases. The genesis of these holes is related to the pull of a detached vitreous on retinovitreal adhesions in Martegiani's area. These tiny holes are closely adjacent to retinal blood vessels and situated predominantly in the lower nasal quadrant. The resulting retinal detachments are shallow, not undulating, mostly stationary or only slowly progressive, and with few exceptions involve the inferior nasal quadrant. Simple glaucoma was found in an unexpected high proportion.
Experimental methods at high rates of strain

Le domaine de recherche qui est le sujet de la revue preente est a la fois vaste et importante, et il est impossible de lui entierement rendre justice dans le cas de cet article. Neanmoins, nous tentons ici de tracer l'histoire de quelques-uns des developpements les plus importants et d'inclure la plupart des techniques majeures. En particulier, nous traitons en detail les sujets suivants: essai de choc de Taylor, test de barre de Hopkinson, et essai d'impact de plaque 1-D. Et finalement, nous discutons le progres recent en photographie ultrarapide ― domaine-clef, en particulier en association avec des techniques optiques
Tomatoes and risk of digestive‐tract cancers

In view of the persisting uncertainty concerning possible mechanisms by which high vegetable and fruit intake decreases cancer risk, foods with divergent values for potentially important micronutrients are a priority for investigation. Tomatoes are low in beta‐carotene, but high in lycopene, an active antioxidative agent. In order to assess the effect of tomatoes on risk of cancers of the digestive tract, data were analyzed from an integrated series of case‐control studies conducted between 1985 and 1991 in northern Italy, where tomato intake is high but, also, heterogeneous. The overall dataset included the following histologically confirmed cancer cases: oral cavity and pharynx, 314; esophagus, 85; stomach, 723; colon, 955; and rectum, 629; and a total of 2,879 controls admitted to hospital for acute non‐neoplastic or non‐digestive conditions, unrelated to long‐term dietary modifications. Multivariate odds ratios (OR) and 95% confidence interval (CI) for subsequent quartiles of intake of raw tomatoes were derived, after allowance for age, sex, study center, education, smoking and drinking level, and tertile of total caloric intake. There was a consistent pattern of protection for all sites (OR in the upper quartile ranging between 0.4 and 0.7), most notably for gastrointestinal neoplasms. All trends in risk were highly significant. The beneficial effect of raw tomatoes in this population may be partly due to the fact that they constitute perhaps the most specific feature of the Mediterranean diet. However, if it is true that tomatoes protect against digestive‐tract cancers, this is of interest from both a scientific and a public health viewpoint.
Phosphorylation of the N-Ethylmaleimide-sensitive Factor Is Associated with Depolarization-dependent Neurotransmitter Release from Synaptosomes*

Critical to SNARE protein function in neurotransmission are the accessory proteins, solubleN-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP), and NSF, that play a role in activation of the SNAREs for membrane fusion. In this report, we demonstrate the depolarization-induced, calcium-dependent phosphorylation of NSF in rat synaptosomes. Phosphorylation of NSF is coincident with neurotransmitter release and requires an influx of external calcium. Phosphoamino acid analysis of the radiolabeled NSF indicates a role for a serine/threonine-specific kinase. Synaptosomal phosphorylation of NSF is stimulated by phorbol esters and is inhibited by staurosporine, chelerythrine, bisindolylmaleimide I, calphostin C, and Ro31-8220 but not the calmodulin kinase II inhibitor, Kn-93, suggesting a role for protein kinase C (PKC). Indeed, NSF is phosphorylated by PKC in vitro at Ser-237 of the catalytic D1 domain. Mutation of this residue to glutamic acid or to alanine eliminates in vitrophosphorylation. Molecular modeling studies suggest that Ser-237 is adjacent to an inter-subunit interface at a position where its phosphorylation could affect NSF activity. Consistently, mutation of Ser-237 to Glu, to mimic phosphorylation, results in a hexameric form of NSF that does not bind to SNAP-SNARE complexes, whereas the S237A mutant does form complex. These data suggest a negative regulatory role for PKC phosphorylation of NSF.
O soli mio: exploring millimeter wave radar for musical interaction

This paper describes an exploratory study of the potential for musical interaction of Soli, a new radar-based sensing technology developed by Google’s Advanced Technology and Projects Group (ATAP). We report on our hands-on experience and outcomes within the Soli Alpha Developers program. We present early experiments demonstrating the use of Soli for creativity in musical contexts. We discuss the tools, workflow, the affordances of the prototypes for music making, and the potential for design of future NIME projects that may integrate Soli.
Treating chronic pain with CBT after a stomach cancer: what strategies can be used?

Dear Editor, We write to make some comments and pertinent observations regarding a recent case report. The International Association for the Study of Pain (IASP) [1] defines pain as “an unpleasant sensory and emotional experience associated with actual or potential tissue damage, or described in terms of such damage. ” The IASP's definition highlights the multidimensional and subjective nature of pain, a complex experience that is unique to each individual. Operant behavior therapy for chronic pain is guided by the original operant conditioning principles proposed by Skinner [2] and refined by Fordyce [3] to be applicable to pain management. In this model, conditioned behaviors occur as a product of learning the consequences of engaging in the given behavior. Cognitive restructuring entails recognition of current maladaptive cognitions the patient is engaging in, challenging of the identified negative cognitions, and reformulation of thoughts to generate balanced, adaptive alternative thoughts. Research [4] has found cognitive behavioral therapy (CBT) to be an effective treatment for chronic pain and its sequelae as marked by significant changes in various domains when compared with wait-list control conditions. A meta-analysis [5] of 52 published studies compared CBT to habitual treatment control conditions and active control conditions at various …
TLR 9-Targeted STAT 3 Silencing Abrogates Immunosuppressive Activity of Myeloid-Derived Suppressor Cells from Prostate Cancer Patients

Purpose—Recent advances in immunotherapy of advanced human cancers underscored the need to address and eliminate tumor immune evasion. The myeloid-derived suppressor cells (MDSCs) are important inhibitors of T cell responses in solid tumors, such as prostate cancers. However, targeting MDSCs proved challenging due to their phenotypic heterogeneity. Experimental Design—Myeloid cell populations were evaluated using flow cytometry on blood samples, functional assays and immunohistochemical/immunofluorescent stainings on tumor/tumor-draining lymph node specimens from healthy subjects, localized and metastatic castration-resistant prostate cancer patients. Results—Here, we identify a population of Lin−CD15HICD33LO granulocytic MDSCs that accumulate in patients’ circulation during prostate cancer progression from localized to metastatic disease. The prostate cancer-associated MDSCs potently inhibit autologous CD8+ T cells proliferation and production of IFNγ and Granzyme-B. The circulating MDSCs have high levels of activated STAT3, which is a central immune checkpoint regulator. The granulocytic pSTAT3+ Correspondence should be addressed to: Marcin Kortylewski, Ph.D., 1500 East Duarte Rd., Duarte, CA 91010; phone: (626) 256-4673 ext.64120; fax: (626) 471-3602; mkortylewski@coh.org or Sumanta K. Pal, Ph.D., 1500 East Duarte Rd., Duarte, CA 91010; phone: (626) 256-4673 ext.68230; fax: (626) 301-8233; spal@coh.org. AUTHORSHIP CONTRIBUTIONS Contribution: D.M.S.H. , D.M. , P.D. , H.W. performed research; D.M.S.H. , M.K. designed experiments; D.M.S.H. , D.M. , J.J., M.D. S.F. , S.K. P. and M.K. analyzed the data; D.M.S.H. and M.K. wrote the manuscript. Conflict-of-interest statement: The authors have declared that no conflict of interest exists. The authors have no conflicting financial interests. HHS Public Access Author manuscript Clin Cancer Res. Author manuscript; available in PMC 2016 August 15. Published in final edited form as: Clin Cancer Res. 2015 August 15; 21(16): 3771–3782. doi:10.1158/1078-0432.CCR-14-3145. A uhor M anscript
ArabRelat: Arabic Relation Extraction using Distant Supervision

Relation Extraction is an important preprocessing task for a number of text mining applications, including: Information Retrieval, Question Answering, Ontology building, among others. In this paper, we propose a novel Arabic relation extraction method that leverages linguistic features of the Arabic language in Web data to infer relations between entities. Due to the lack of labeled Arabic corpora, we adopt the idea of distant supervision, where DBpedia, a large database of semantic relations extracted from Wikipedia, is used along with a large unlabeled text corpus to build the training data. We extract the sentences from the unlabeled text corpus, and tag them using the corresponding DBpedia relations. Finally, we build a relation classifier using this data which predicts the relation type of new instances. Our experimental results show that the system reaches 70% for the F-measure in detecting relations.
Resection of previously unresectable liver metastases from colorectal cancer (LMCRC) after chemotherapy (CT) with CPT-11/L-OHP/LV5FU (Folfirinox): A prospective phase II trial.

3613 Background: 5 year-survival after LMCRC resection is 30-35%. Folfiri and Folfox are standard regimens in MCRC. The triple combination Folfirinox as induction CT could improve tumor shrinkage and therefore could allow resection of LM in unresectable patients (pts). METHODS Based on a multidisciplinary consensus, unresectability is defined as follows: LM in right or left lobe in contact of controlateral liver pedicle; LM in contact of veina cava or involving 2 hepatic veins and in contact with the third; liver remaining mass less than 25-30% of functional liver; no initial forseen optimal resection, but susceptible to become resectable after shrinkage. Biweekly 48h-cycles (Cy): L-OHP 85 mg/m2, CPT-11 180 mg/m2, LV5FU were administered. An Independent Committee (IC) reviewed pts eligibility and RR. The primary endpoint was resectability R0 defined as R0= margin≥2mm, R1=margin<2mm, Ra= resection + either cryosurgery or radiofrequency. RESULTS Among 34 pts included, 25 were eligible as per IC. To date, 24 pts out of 25 were assessable for primary endpoint. Median age=57y [39-69], M/F=15/10, PS 0/1=17/8, metachronous LM=12 & synchronous LM=13. After a median number of 6 Cy [4-10] before surgery, the overall RR was 72% (CR=1, PR=17, MR=3, SD=1). Pathological status after surgery was: R0=9, R1=5, Ra=6. 2 pts had cryosurgery only and 2 pts were not operated upon. Thus 22 pts had complete local treatment and 14 pts could undergo surgical resection R0-R1. Post-surgical complication =2 biliary fistula, 2 infections leading to hospitalization were reported. After resection, the same CT was pursued in adjuvant setting till 12 Cy in 11 pts. CONCLUSIONS In this population who initially could not undergo optimal resection, 37.5% of assessable pts underwent R0 resection and 91.7% could receive complete local treatment with resection and / or ablation procedures after active CT. [Table: see text].
Three-Level Anterior Cervical Discectomy and Fusion With Plate Fixation: Radiographic and Clinical Results

Study Design. Retrospective review of patients with cervical spondylosis treated with 3-level anterior cervical discectomy and fusion with plate fixation. Objective. To assess the radiographic and clinical outcome of 3-level instrumented anterior cervical discectomy. Summary of Background Data. Three-level cervical discectomy without plate fixation has shown high rates of pseudarthrosis and poor outcomes. The addition of internal fixation may improve these parameters. Methods. A total of 46 patients were observed for an average of 17.6 months (range, 6–51 months). All had 3-level anterior cervical discectomy and fusion with tricortical iliac crest autograft (4 cases), fibular ring allograft (38 cases), or titanium cage (4 cases). Allografts and cages were filled with iliac crest autograft. All patients had semirigid plating. Clinical and radiographic follow-up data were obtained. Clinical outcomes were measured as described by Robinson and with the Nurick scale. Results. Forty-four patients achieved solid fusion. Two patients had additional surgery for junctional disease, and in 1 of them pseudarthrosis repair was also performed. One asymptomatic pseudarthrosis was noted. With a successful result defined as an excellent or good outcome accompanied by significant pain relief, 38 patients had a successful result (83%). Radiographic adjacent level disease was diagnosed in 11 patients after surgery and was symptomatic in 5. Conclusion. Three-level anterior cervical discectomy with plate fixation has a high rate of fusion, a low complication rate, and acceptable outcome in the treatment of multilevel cervical spondylosis.
Selectively formed high mobility strained Ge PMOSFETs for high performance CMOS

For the first time, we have integrated strained germanium (s-Ge) channel PMOSFETs with conventional CMOS processes including shallow trench isolation (STI) and scaled thin gate dielectrics. The selectively formed thin s-Ge channels are realized on pre-patterned SiGe on insulator (SGOI) regions by local thermal mixing (TM) or selective UHVCVD process. The thinnest SiO/sub 2/ on the s-Ge is achieved by low temperature remote plasma oxidation of a thin Si cap. As a result, 3X drive current enhancement is demonstrated on the fabricated s-Ge channel PMOSFETs over the Si controls. In addition, an appropriate threshold voltage (Vth) is demonstrated on the HfO/sub 2//P+ poly Si gate PMOSFETs when using an s-Ge channel.