Miracle-dz/UniFAR-FTUs huggingface dataset:
PANX2 and brain lower grade glioma genesis: A bioinformatic analysis

PANX2 forms large-pore channels mediating ATP release in response to physiological and pathological stimuli. Although PANX2 shows involvements in glioma genesis, the underlying mechanism remains unclear. PANX2 mRNA expression was analyzed via Oncomine and was confirmed via Gene Expression Profiling Interactive Analysis (GEPIA). The influence of PANX2 on overall survival (OS) of glioma was evaluated using LinkedOmics and further assessed through Cox regression analysis. The correlated genes with PANX2 acquired from LinkedOmics were validated through GEPIA and cBioPortal. Protein-protein interaction (PPI) of these genes was then obtained using Search Tool for the Retrieval of Interacting Genes (STRING) and Cytoscape with MCODE plug-in. All the PANX2-related genes underwent Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. The correlation between PANX2 and cancer immune infiltrates was evaluated via Tumor Immune Estimation Resource (TIMER). A higher expression of PANX2 only revealed a better OS in brain low grade glioma (LGG). PANX2-related genes in LGG functionally enriched in neuroactive ligand-receptor interaction, synaptic vesicle cycle, and calcium signaling. The hub genes from highest module of PPI were mainly linked to chemical synaptic transmission, plasma membrane, neuropeptide, and the pathway of neuroactive ligand-receptor interaction. Besides, PANX2 expression was negatively associated with infiltrating levels of macrophage, dendritic cells, and CD4+ T cells. This study demonstrated that PANX2 likely participated in LGG pathogenesis by affecting multiple molecular pathways and immune-related processes. PANX2 was associated with LGG prognosis and might become a promising therapeutic target of LGG.
Mechanisms and regulation underlying membraneless organelle plasticity control

Abstract Evolution has enabled living cells to adopt their structural and functional complexity by organizing intricate cellular compartments, such as membrane-bound and membraneless organelles (MLOs), for spatiotemporal catalysis of physiochemical reactions essential for cell plasticity control. Emerging evidence and view support the notion that MLOs are built by multivalent interactions of biomolecules via phase separation and transition mechanisms. In healthy cells, dynamic chemical modifications regulate MLO plasticity, and reversible phase separation is essential for cell homeostasis. Emerging evidence revealed that aberrant phase separation results in numerous neurodegenerative disorders, cancer, and other diseases. In this review, we provide molecular underpinnings on (i) mechanistic understanding of phase separation, (ii) unifying structural and mechanistic principles that underlie this phenomenon, (iii) various mechanisms that are used by cells for the regulation of phase separation, and (iv) emerging therapeutic and other applications.
Effects of entrepreneurial orientation on social media adoption and SME performance: The moderating role of innovation capabilities

The increase of social media (SM) has led to continuous deviations in how day-to-day entrepreneurial activities can be carried out. Additionally, studies devoted to SM entrepreneurship and SM are relatively limited and fragmented in their focus. However there is growing interest from scholars, practitioners and academia for upcoming studies and exploration within small and medium-sized enterprises (SMEs) context. This research explores the impact of entrepreneurial orientation (EO) on SM adoption and SME performance in developing countries. We employed the resource-based view (RBV) as the foundation for developing the conceptual framework The present study employed a closed-ended questionnaire to collect data from SMEs located in Pakistan. Partial-least-squares-structural equation-modeling (PLS-SEM) was utilized for the analysis of 423 responses. The results proved a direct positive link between EO–SMEs performance, EO–SM adoption, SM adoption–SMEs performance, innovation capabilities (IC), and SME performance. Partial mediation was found between EO and SME performance, and the significant moderation effect of IC was found between SM adoption and SME performance. This paper has implications for practitioners and researchers regarding SM adoption in the SMEs. It builds an empirical, multi-dimensional hypothesized model, including mediating and moderating roles affecting the relationships.
Is frozen embryo transfer better than fresh embryo transfer in women undergoing intracytoplasmic sperm injection over the age of thirty-five? A single referral centre experience

Abstract Developments in embryo freezing techniques in recent years have increased the chance of pregnancy after frozen embryo transfers. In this study we aimed to compare the pregnancy outcomes between fresh (ET) and frozen-thawed embryo transfer (FET) and to evaluate the benefits of embryo freezing strategy for a woman of advanced age. A total of 513 ET cycles in women over the age of 35 years, including 397 fresh ET cycles and 116 FET cycles were reviewed. Mean age was 37.7 ± 2.91 years in FET cycles and 38 ± 2.8 in fresh cycles (p = .327). The patients undergoing FET cycles had higher oestradiol levels and progesterone levels on the day of trigger in their previous fresh cycles compared to that of fresh ET cycles (1675 pmol/L versus 991 pmol/L; 1.43 pmol/L versus 0.96 pmol/L; p < .005, respectively). Biochemical pregnancy rates (43% versus 32%; p = .048), clinical pregnancy rates (38% versus 29%; p = .030) and live birth rates (30% versus 19.6%; p = .013) were significantly higher in the FET than in the fresh ET over 35 years of age. IMPACT STATEMENT What is alreday known on this subject? Ovarian stimulation commonly results in the generation of more embryos than are necessary for the fresh embryo transfer. Therefore, cryopreservation and subsequent replacement of frozen–thawed embryos is an integral part of assisted reproductive technique (ART) programs. As IVF technology improved, embryo freezing was performed to allow subsequent transfer if the fresh cycle was unsuccessful. What do the results of this study add? Clinical pregnancy rates and live birth rates were found to be higher in frozen thawed embryo transfer group compared to fresh embryo transfer group over the age of 35. What are the implications of these findings for clinical practice and/or further research? Frozen thawed embryo transfer seems to be a reasonable and favourable method compared to fresh embryo transfer in patients over 35 years.
Dynamics and healing behavior of metallosupramolecular polymers

Metal ion tracking allows monitoring of the healing process in supramolecular polymers on the nanoscale. Self-healing or healable polymers can recuperate their function after physical damage. This process involves diffusion of macromolecules across severed interfaces until the structure of the interphase matches that of the pristine material. However, monitoring this nanoscale process and relating it to the mechanical recovery remain elusive. We report that studying diffusion across healed interfaces and a correlation of contact time, diffusion depth, and mechanical properties is possible when two metallosupramolecular polymers assembled with different lanthanoid salts are mended. The materials used display similar properties, while the metal ions can be tracked with high spatial resolution by energy-dispersive x-ray spectrum imaging. We find that healing actual defects requires an interphase thickness in excess of 100 nm, 10 times more than previously established for self-adhesion of smooth films of glassy polymers.
Correction

LETTER (ONLINE ONLY). For the letter ‘‘Reply to Blazer et al.: Flawed challenges to ‘Acetylcholinesterase inhibitors and Gulf War illnesses’,’’ by Beatrice A. Golomb, which appeared in issue 33, August 19, 2008, of Proc Natl Acad Sci USA (105:E53; first published August 11, 2008; 10.1073 pnas.0805246105), the author notes that the following sentences were omitted from the end of the first paragraph of the letter: ‘‘Their statements are counter to fact. They mischaracterize the evidence, employ the introduction of irrelevant material, and exemplify flaws in inference, as I will illustrate. ’’ The corrected letter appears below.
Complete Closed Genome Sequence of the Inulin-Utilizing Lactiplantibacillus plantarum Strain Lp900, Obtained Using a Hybrid Nanopore and Illumina Assembly

Lactiplantibacillus plantarum is a genetically and phenotypically diverse species of lactic acid bacteria. We announce the hybrid de novo assembly of Oxford Nanopore Technologies and Illumina DNA sequence reads, producing a closed circular chromosome of 3,206,992 bp and six plasmids of the inulin-utilizing L. plantarum strain Lp900. ABSTRACT Lactiplantibacillus plantarum is a genetically and phenotypically diverse lactic acid bacterium species. We announce the hybrid de novo assembly of Oxford Nanopore Technologies and Illumina DNA sequence reads, producing a closed circular chromosome of 3,206,992 bp and six plasmids of the inulin-utilizing L. plantarum strain Lp900.
Draft Genome Sequences of Three Strains of Campylobacter jejuni Isolated from Patients with Guillain-Barré Syndrome in Bangladesh

Campylobacter jejuni is the pathogen most commonly associated with Guillain-Barré syndrome (GBS). The present work describes the draft genome sequences of 3 C. jejuni strains, BD39, BD67, and BD75, isolated from stool specimens of C. jejuni-triggered patients with GBS using Illumina technologies. ABSTRACT Campylobacter jejuni is the pathogen most commonly associated with Guillain-Barré syndrome (GBS). The present work describes the draft genome sequences of 3 C. jejuni strains, BD39, BD67, and BD75, isolated from stool specimens of patients with C. jejuni-triggered GBS using Illumina technologies.
Complete Genome Sequence of an Antimicrobial-Producing Bacillus velezensis Sam8H1 Isolate from the Makgadikgadi Saltpans of Botswana

The global antimicrobial drug resistance crisis requires urgency in searching for more effective broad-spectrum antimicrobial drugs. Here, we present a complete circular genome sequence and a plasmid of an antimicrobial-producing isolate, Bacillus velezensis strain Sam8H1, from the Makgadikgadi saltpans in Botswana. Bioinformatic analyses revealed 12 putative secondary metabolite biosynthetic gene clusters important for genome-guided drug discovery studies. ABSTRACT The global antimicrobial drug resistance crisis requires urgency in searching for more effective broad-spectrum antimicrobial drugs. Here, we present a complete circular genome sequence and a plasmid of an antimicrobial-producing isolate, Bacillus velezensis strain Sam8H1, from the Makgadikgadi saltpans in Botswana. Bioinformatic analyses revealed 12 putative secondary metabolite biosynthetic gene clusters important for genome-guided drug discovery studies.
CONSORT extension for the reporting of randomised controlled trials conducted using cohorts and routinely collected data (CONSORT-ROUTINE): checklist with explanation and elaboration

Randomised controlled trials are increasingly conducted as embedded, nested, or using cohorts or routinely collected data, including registries, electronic health records, and administrative databases, to assess if participants are eligible for the trial and to facilitate recruitment, to deliver an embedded intervention, to collect trial outcome data, or a combination of these purposes. This report presents the Consolidated Standards of Reporting Trials (CONSORT) extension for randomised controlled trials conducted using cohorts and routinely collected data (CONSORT-ROUTINE). The extension was developed to look at the unique characteristics of trials conducted with these types of data with the goal of improving reporting quality in the long term by setting standards early in the process of uptake of these trial designs. The extension was developed with a sequential approach, including a Delphi survey, a consensus meeting, and piloting of the checklist. The checklist was informed by the CONSORT 2010 statement and two reporting guidelines for observational studies, the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement and the REporting of studies Conducted using Observational Routinely collected Data (RECORD) statement. The extension includes eight items modified from the CONSORT 2010 statement and five new items. Reporting items with explanations and examples are provided, including key aspects of trials conducted using cohorts or routinely collected data that require specific reporting considerations.
A methodology and framework for software memoization of functions

Enhancing performance is crucial when developing applications for high-performance and embedded computing. It requires sophisticated techniques and in-depth knowledge of the application domain and target architecture. Typically, developers prioritize the application's functional requirements over extra-functional requirements. Thus, a large part of the optimization effort is shifted to performance engineers, who rely on manual effort, alongside many analysis and optimization tools that need integration. This paper focuses on memoization, which caches results of pure computations and retrieves them if a function is called with repeating arguments. We propose a methodology for allowing developers and performance engineers to apply memoization straightforwardly by automating code analysis, code transformations, and memoization-specific profiling. It helps developers with no optimization expertise to quickly set up memoization and, simultaneously, it provides performance engineers with highly customizable analysis and memoization. We provide a concrete implementation supported by a DSL, a source-to-source compiler, and a memoization framework. We evaluate the methodology and framework with publicly available benchmarks. We show how one can analyze applications to select functions with performance improvement potential, which the experiments reveal might be challenging to find, and improve some applications with minimal effort.
High Resolution Synchrotron Diffraction Study on Charge Density Distribution of Ampicillin Trihydrate

Charge density distribution in ampicillin trihydrate was investigated experimentally. Results were compared with the quantum calculations using density functional theory. The charge derived properties including Mulliken atomic charges, dipole moment, and molecular electrostatic potential were calculated. The multipole analysis was done for the refinement of experimental population parameters. The structure factors obtained from multipole treatment were used for the construction of Fourier maps. Topological properties of the charge distribution were discussed and the characteristics of (3, −1) critical points were analyzed.Charge density distribution in ampicillin trihydrate was investigated experimentally. Results were compared with the quantum calculations using density functional theory. The charge derived properties including Mulliken atomic charges, dipole moment, and molecular electrostatic potential were calculated. The multipole analysis was done for the refinement of experimental population parameters. The structure factors obtained from multipole treatment were used for the construction of Fourier maps. Topological properties of the charge distribution were discussed and the characteristics of (3, −1) critical points were analyzed.
Effects of Different Temperature and Light Treatments on Photosynthetic System II in Vitis vinifera L. cv. Cabernet Sauvignon

【Objective】The combined effects of different temperature and light treatments on the operation of photosynthetic apparatus were investigated to reveal the mechanism of inhibition of photosynthesis. 【Method】One-year-old Cabernet Sauvignon grapevines were treated with different temperatures(28,34,37 and 40℃)combined with two kinds of light intensities(800,1600 μmol? m-2? s-1). Gas exchange and chlorophyll a fluorescence parameters were simultaneously analyzed to investigate the effects of different treatments on the PSII function【Result】Despite the treated light intensities,as temperature increased,net photosynthetic rate(Pn),stomatal conductance(Gs),actual PSⅡ efficiency(ΦPSII),electron transport rate(ETR),performance index(PIABS),density of RCs(RC/CS) and the probability that a trapped exciton moves an electron into the electron transport chain beyond QA(ΨEo) decreased;PSⅡ excitation pressure(1-qP),non-photochemical quenching(NPQ) and approximated initial slope of the fluorescence transient(Mo) increased.Under the moderate light intensities,the decrease of Pn was due mainly to stomatal limitation during the temperature from 28℃ to 37℃,and the xanthophyll cycle played an increased role to protect photosystem II.Under strong light stress,28℃ treatment decreased Pn to the level of the 40℃ treatment of moderate light intensities,meanwhile PIABS reached a lower level. 【Conclusion】Under moderate light intensities,the PSⅡacceptor sides were not injured until the temperature reached 37℃;strong light stress,however,began to injure the PSⅡ acceptor sides from 28℃,when the temperature reached or exceeded 37℃,the density of RCs and the acceptor sides were damaged severely which showed PSⅡcould be more susceptible to strong light than moderate heat temperature.
Social robot deception and the culture of trust

Abstract Human beings are deeply social, and both evolutionary traits and cultural constructs encourage cooperation based on trust. Social robots interject themselves in human social settings, and they can be used for deceptive purposes. Robot deception is best understood by examining the effects of deception on the recipient of deceptive actions, and I argue that the long-term consequences of robot deception should receive more attention, as it has the potential to challenge human cultures of trust and degrade the foundations of human cooperation. In conclusion: regulation, ethical conduct by producers, and raised general awareness of the issues described in this article are all required to avoid the unfavourable consequences of a general degradation of trust.
Review on nanocomposites based on aerospace applications

Abstract Advanced materials were used and are being implemented in structural, mechanical, and high-end applications. Contemporary materials are used and being implemented in structural, mechanical, and high-end applications. Composites have several major capabilities, some of them being able to resist fatigue, corrosion-resistance, and production of lightweight components with almost no compromise to the reliability, etc. Nanocomposites are a branch of materials within composites, known for their greater mechanical properties than regular composite materials. The use of nanocomposites in the aerospace industry currently faces a research gap, mainly identifying the future scope for application. Most successes in the aerospace industry are because of the use of suitable nanocomposites. This review article highlights the various nanocomposite materials and their properties, manufacturing methods, and their application, with key emphasis on exploiting their advanced and immense mechanical properties in the aerospace industry. Aerospace structures have used around 120,000 materials; herein, nanocomposites such as MgB2, multi-walled carbon nanotubes, and acrylonitrile butadiene styrene/montmorillonite nanocomposites are discussed, and these highlight properties such as mechanical strength, durability, flame retardancy, chemical resistance, and thermal stability in the aerospace application for lightweight spacecraft structures, coatings against the harsh climate of the space environment, and development of microelectronic subsystems.
Small rodent communities (Muridae) in Gabonese savannas: species diversity and biogeographical affinities

Abstract The study contributes to the knowledge of species composition and biogeographical affinities of savannas rodent in Gabon. Unlike small rodents in Gabonese forests, there is little data on the diversity of small rodents in Gabonese savannas. The diversity and distribution of rodent murid communities was studied in four different types of savanna in Gabon: Coastal Basin (South-West), Lopé/Okanda (in the Center), Batéké Plateaux (Southeastern) and Ngougnié/ N’yanga (in the South). A total of 428 individuals representing six species were captured over 11,920 trap nights. Trap success was highly variable (2.2–6.9 %). The most abundant species were Mus minutoides (69%) followed by Lemniscomys striatus (21.5%). Indices of species richness varied from 2 to 5 and diversity (Shannon and Weaver) was low in the four savannas with the highest value at Ngougnié/N’yanga (H′ = 1.2). Species distributions show that Gabonese savanna small rodents conform to four distribution types, with one species known from Zambesian savannas exhibiting austral affinities (Pelomys campanae: occurs in three southern savannas). This new information provides important insight into the biogeography of small rodents at a local and regional level. Moreover, the correspondence analysis highlighted an influence of local ecological factors on population abundance.
Small Sample Adjustment for Hypotheses Testing on Cointegrating Vectors

Abstract Johansen’s (2000. “A Bartlett Correction Factor for Tests of on the Cointegrating Relations.” Econometric Theory 16: 740–78) Bartlett correction factor for the LR test of linear restrictions on cointegrated vectors is derived under the i.i. d. Gaussian assumption for the innovation terms. However, the distribution of most data relating to financial variables is fat-tailed and often skewed; there is therefore a need to examine small sample inference procedures that require weaker assumptions for the innovation term. This paper suggests that using the non-parametric bootstrap to approximate a Bartlett-type correction provides a statistic that does not require specification of the innovation distribution and can be used by applied econometricians to perform a small sample inference procedure that is less computationally demanding than it’s analytical counterpart. The procedure involves calculating a number of bootstrap values of the LR test statistic and estimating the expected value of the test statistic by the average value of the bootstrapped LR statistic. Simulation results suggest that the inference procedure has good finite sample property and is less dependent on the parameter space of the data generating process.
Morph-specific patterns of sex organ positions in species with style length polymorphism

In style length polymorphism, morph-specific sequence of encountering male and female sex organs within a flower by pollinators can cause differences in the need to avoid self-pollination and encourage inter-morph pollination. We asked if this difference can lead to disparity in stigma-anther separation (herkogamy) between morphs and spatial match between sex organs of complementary morphs (reciprocity). Further, we tested if herkogamy, and hence the level of selfing, is fairly constant among individuals of a population. Additionally, we examined the relationship between herkogamy and reciprocity among individuals of a population to understand functional interactions between these two morphological traits. Using data on sex organ heights for >200 heterostylous species from the literature, we observed that the short-styled morph had higher herkogamy as compared to the long-styled morph indicating a higher need to avoid selfing. Reciprocity did not show a consistent difference between the upper and lower sex organs implying a strong influence of local ecological factors. In most populations, allometric relationships suggested that herkogamy and hence the level of selfing remains constant. Finally, we observed that herkogamy and reciprocity can be related among individuals of a population, sometimes indicating a potential trade-off between avoidance of self-pollination and facilitation of inter-morph pollination.