Miracle-dz/UniFAR-FTUs huggingface dataset:
Asian Pacific Journal of Tropical Biomedicine U tilization of multiple "omics" studies in microbial pathogeny for microbiology insights

I n the present day, bioinformatics becomes the modern science with several advantages. S everal new “ omics ” sciences have been introduced for a few years and those sciences can be applied in biomedical work. H ere, the author will summarize and discuss on important applications of omics studies in microbiology focusing on microbial pathogeny. I t can be seen that genomics and proteinomics can be well used in this area of biomedical studies.
Highly available cluster: a case study

The methodology and design of a system that provides highly available data in a cluster is presented. A highly available cluster consists of multiple machines interconnected by a common bus. Data is replicated at a primary and one or more backup machines. Data is accessed at the primary, using a location independent mechanism that ensures data integrity. If the primary copy of the data fails, access is recovered by switching to a backup copy. Switchover is transparent to the application, hence called seamless switchover. The fault model is fail-stop. The entire cluster is resilient to at least single failures. Designating data as highly available is selective in scope, and the overhead of replication and recovery is incurred only by applications that access highly available data. An experimental prototype was implemented using IBM AS/400 machines and a high-speed bus with fiber-optic links. <<ETX>>
Numerical and experimental radar cross section analysis of the quadrocopter DJI Phantom 2

This paper presents a numerical and experimental scattering analysis of the quadrocopter DJI Phantom 2 at 10 GHz. Major goal of the study is to evaluate the suitability of simplified quadrocopter models for numerical radar cross section (RCS) investigations. Mono- and bistatic RCS results are obtained by the finite element method and the method of moments. As structural parts of the quadrocopter are made of plastic and electronic components consist of metal, focus is put on the numerical modeling of mixed dielectric-metallic objects. Thereby, different materials and model resolutions are examined and their influence on the scattered field is analyzed. Moreover, we investigate the impact of approximated excitations as well as object misalignments in order to quantify potential error sources. Numerical results are compared with measurements which have been carried out in a anechoic chamber. Deviations to measurements are discussed and remedies are proposed.
Physician recommendations for diet and physical activity: which patients get advised to change?

BACKGROUND National objectives and guidelines call upon physicians to help reduce the population burden of chronic diseases by advising patients to eat less fat and get more physical activity. However, studies show physicians are most likely to provide behavioral recommendations to patients who are already sick. Understanding factors that influence physicians' advising decisions can help broaden the reach of these activities. METHODS Subjects were 915 adult patients and 27 physicians from four community-based family medicine clinics in southeastern Missouri. To participate, patients completed a self-administered behavioral and health questionnaire while waiting to see their doctor. RESULTS Having a high body mass index was the strongest predictor of receiving advice to increase physical activity (OR = 1.6; 95% CI 1.3, 2.0), and having a high cholesterol level was the strongest predictor of receiving advice to eat less fat (OR = 1.9; 95% CI 1.5, 2.4). Neither the actual content of patients' diets nor their levels of physical activity were associated with receiving advice. CONCLUSIONS Physicians' advising may be guided by quick but fallible heuristics that systematically exclude patients whose needs are not easily visible. This pattern misses the opportunity to reduce future needs for therapeutic counseling by taking preventive action now.
Memory attestation of wireless sensor nodes through trusted remote agents

Wireless sensor networks (WSNs) have been deployed in various commercial, scientific, and military applications for surveillance and critical data collection. A serious threat to sensor nodes is malicious code injection attack that results in fake data delivery or private data disclosure. Memory attestation used for verifying the integrity of a device's firmware is a promising solution for detecting an infected sensor node; particularly, low-cost software-based schemes are suitable for protecting resource-constrained sensor nodes. However, a software-based attestation usually requires some additional mechanisms for providing reliable integrity evidence when the sensor nodes communicate with the verifier through a multihop setting. Alternative hardware-based attestation (e.g. trusted platform module) ensures a reliable integrity measurement that, however, is impractical for certain WSN applications primarily because of the high computational overhead and high hardware cost. The authors propose a lightweight hardware-based memory attestation scheme against the malicious code injection attack, and the proposed scheme employs a lightweight tamper-resistant hardware security module, which is free from any complicated cryptographic computation and is particularly suitable for low-cost sensor nodes. In addition, experimental results demonstrating the effectiveness of the proposed scheme are presented.
Challenges with bearings only tracking for missile guidance systems and how to cope with them.

This paper addresses the problem of closed loop missile guidance using bearings and target angular extent information. Comparison is performed between particle filtering methods and derivative free methods. The extent information characterizes target size and we show how this can help compensate for observability problems. We demonstrate that exploiting angular extent information improves filter estimation accuracy. The performance of the filters has been studied over a testing scenario with a static target, with respect to accuracy, sensitivity to perturbations in initial conditions and in different seeker modes (active, passive and semi-active).
Recognition of heptameric seed sequence underlies multi‐target regulation by RybB small RNA in Salmonella enterica

Prokaryotic regulatory small RNAs act by a conserved mechanism and yet display a stunning structural variability. In the present study, we used mutational analysis to dissect the functional anatomy of RybB, a σE‐dependent sRNA that regulates the synthesis of major porins in Escherichia coli and Salmonella. Mutations in the chromosomal rybB locus that altered the expression of an ompC–lac fusion were identified. Some of the mutations cluster within a seven‐nucleotide segment at the 5′ end of the sRNA and affect its ability to pair with a sequence 40 nucleotides upstream from ompC translation start site. Other mutations map near the 3′ end of RybB, destabilizing the sRNA or altering its binding to Hfq. The 5′ end of RybB is also involved in ompD regulation. In this case, the sRNA can choose between two mutually exclusive pairing sites within the translated portion of the mRNA. Some of the RybB 5′ end mutations affect the choice between the two sites, resulting in regulatory responses that diverge from those observed in ompC. Further analysis of RybB target specificity identified chiP (ybfM), a gene encoding an inducible chitoporin, as an additional member of the RybB regulon. Altogether, our results indicate that an heptameric ‘seed’ sequence is sufficient to confer susceptibility to RybB regulation.
Genetic correlation and path coefficient analysis for yield components and fibre related traits in CMS based hybrids in cotton (Gossypium hirsutum L.).

Eighty one, F1 cotton (Gossypium hirsutum L.) hybrids were analysed for genetic variability, correlation and path coefficients. High genetic variability combined with high heritability and genetic advance was observed for seed cotton yield, number of bolls, boll weight, harvest index and micronaire value which shows that these traits will respond to selection based on phenotypic performance. Estimates of correlation and path coefficient analysis showed that plant height, number of bolls, boll weight, harvest index, number of monopods and 2.5% span length had significant positive association with seed cotton yield. Also number of bolls, boll weight and harvest index had positive direct effect on seed cotton yield whereas plant height, number of sympods, number of monopods, 2.5% span length and days to maturity contributed mainly through number of bolls. Thus, selection on the basis of number of bolls, boll weight and harvest index will be effective in improving the seed cotton yield.
NON-PHOTOREALISTIC VIDEO SYNTHESIS FOR ARTISTIC USER EXPERIENCE ON MOBILE DEVICES

This paper presents analysis/synthesis strategies for generating abstract, creative representations via the camera input on a mobile device. Mobile devices are well suited for interactive video processing since they are simultaneously capable of image capture, display, and manipulation. Analysis/synthesis methods are particularly powerful in interactive arts projects as they enable even drastic manipulations of the input image while still maintaining fundamental aspects of its original identity. Moreover, by using abstract synthesis elements (i.e., coherent elements larger than single pixels), we are able to directly interact with the image and to manipulate its final output. We describe some of the exciting capabilities of video processing and interaction on mobile devices and introduce a series of mobile applications that use analysis/synthesis techniques.
Computationally efficient 3D FEM rotor eddy-current loss calculation for permanent magnet synchronous machines

Rotor eddy-current loss calculation by means of 3D finite element analysis is often necessary during later design stages of a new machine, but can still be very time-consuming. This paper proposes two FEM-based methods that potentially require less time to solve than a transient model of the complete machine geometry and are relatively straightforward to implement. Both methods apply an airgap boundary condition obtained from a limited number of solutions of a full 3D model, to a model containing only the rotor geometry. An important property of both methods is that they can account for shielding effects in the rotor. The performance of the methods in terms of accuracy and speed is demonstrated and guidelines for implementation are presented, showing that the rotor losses can be calculated 2-3 faster without loss of accuracy. Finally, using the methods a number of rotor loss trends are confirmed.
Cutting Edge: Basophils Are Transiently Recruited into the Draining Lymph Nodes during Helminth Infection via IL-3, but Infection-Induced Th2 Immunity Can Develop without Basophil Lymph Node Recruitment or IL-3

Basophils are recognized as immune modulators through their ability to produce IL-4, a key cytokine required for Th2 immunity. It has also recently been reported that basophils are transiently recruited into the draining lymph node (LN) after allergen immunization and that the recruited basophils promote the differentiation of naive CD4 T cells into Th2 effector cells. Using IL-3−/− and IL-3Rβ−/− mice, we report in this study that the IL-3/IL-3R system is absolutely required to recruit circulating basophils into the draining LN following helminth infection. Unexpectedly, the absence of IL-3 or of basophil LN recruitment played little role in helminth-induced Th2 immune responses. Moreover, basophil depletion in infected mice did not diminish the development of IL-4–producing CD4 T cells. Our results reveal a previously unknown role of IL-3 in recruiting basophils to the LN and demonstrate that basophils are not necessarily associated with the development of Th2 immunity during parasite infection.
TwiInsight: Discovering Topics and Sentiments from Social Media Datasets

Social media platforms contain a great wealth of information which provides opportunities for us to explore hidden patterns or unknown correlations, and understand people's satisfaction with what they are discussing. As one showcase, in this paper, we present a system, TwiInsight which explores the insight of Twitter data. Different from other Twitter analysis systems, TwiInsight automatically extracts the popular topics under different categories (e.g., healthcare, food, technology, sports and transport) discussed in Twitter via topic modeling and also identifies the correlated topics across different categories. Additionally, it also discovers the people's opinions on the tweets and topics via the sentiment analysis. The system also employs an intuitive and informative visualization to show the uncovered insight. Furthermore, we also develop and compare six most popular algorithms - three for sentiment analysis and three for topic modeling.
“Needlescopic” Heller Myotomy

MIS continues to evolve with the introduction of new techniques and technology. This report discusses the use of “needlescopic” technology in the surgical management of achalasia. Heller myotomy procedures performed between January 1, 1997, and July 1, 2000, were analyzed and the results of 14 needlescopic procedures were compared with 15 laparoscopic procedures. Demographic and short-term outcome data were compared for each group using &khgr;2, Fisher exact, and Student t tests where appropriate. Both groups were similar in age and gender. However, the needlescopic group weighed less (72.2 vs. 83.5 kg;P = 0.05). Intraoperatively, the needlescopic procedures were shorter (98.2 vs. 131.9 minutes;P = 0.03). There were no conversions to open surgery or differences in the number of intraoperative complications for either group. Postoperatively, the groups had similar complications, time to normal diet, and analgesia requirements. Nonetheless, the needlescopic group had a shorter length of stay in hospital (1.1 vs. 2.0 days;P = 0.04). Needlescopic Heller myotomy appears to be a safe treatment option, resulting in a decreased length of stay and improved wound cosmesis.
Encapsulation of mammalian cell with chitosan‐CMC capsule

Viable hybridoma cells were encapsulated. The capsules were formed in one step by placing a drop of cell suspension mixed with negatively charged carboxymethylcellulose (CMC) into a positively charged chitosan solution through the interpolymeric ionic interaction between two oppositely charged polymers. These capsules were found to have a mean diameter of about 1. 5 mm and wall thickness of 3 μm. The cells grew in the capsules using supplemented DMEM/F12 (four kinds of growth factor). The maximum cell density in encapsulating cell culture reached 1 × 107 cells/ml, 10 times higher than that obtained in the free cell culture. The maximum monoclonal antibody concentration in the free cell culture was 15μg/mL, but that in the capsule was 45μg/mL The antibody produced by the cell was concentrated about four times higher inside than outside of the capsules.
Modelado Orientado a Objetos Aplicado a Entornos de Desarrollo Relacionales

En este trabajo se presentan las ventajas del uso del Modelo Orientado a Objetos para el modelado conceptual y la implementacion de sistemas de informacion. La mayoria de las metodologias actuales [Rum91], [Boo94], [BRJ96] carecen de guias precisas y rigurosas (desde el punto de vista del proceso de especificacion) que nos permitan obtener productos finales a partir de los modelos conceptuales construidos. Nuestro trabajo intenta cubrir dichas carencias proporcionando un enfoque metodologico (OO-Method [Pas96], [Pas97]) que abarca la construccion de modelos de analisis y la definicion de un patron de traduccion preciso de estos modelos a esquemas de bases de datos relacionales avanzados. Este articulo presenta un proceso de traduccion facil y preciso a la vez, que nos va a permitir implementar los modelos conceptuales de OO-Method sobre un entorno de desarrollo relacional (usando ORACLE v7 como servidor de datos y Developer/2000 como herramienta de desarrollo), explicando como trasladar los aspectos estaticos y dinamicos recogidos durante la fase de analisis, a estructuras relacionales y codigo para constituir el producto software final.
The application of spiral model in enterprise risk management

The systematic risk management is of great importance in increasing enterprises' interests. With rapid development of economy and informatization and the expansion of enterprise, enterprises are faced with more and more risks. Therefore, it is vital and urgent for enterprises to establish risk management system. The paper analyzes the process of enterprise risk management based on the theory and methods of spiral model, and proposes that the Spiral Model can improve risk management level in enterprises and create economic benefits.
How can we get access to the experiences of people with dementia?

This paper suggests and discusses a methodological approach that aims to facilitate the inclusion of people with dementia in research where the ambition is to better understand their experiences. People with dementia have commonly not been included as informants in research as their cognitive deficits have been regarded as a hindrance. Moreover, in the qualitative research tradition, most inquiries rely on data obtained from interviews, thereby requiring communication and verbal skills, which are skills that are affected early in the cause of dementia. Consequently, the considerably under-researched area of how dementia is experienced and managed from the perspective of those afflicted has long been put aside. This paper suggests that a combination of qualitative observations and adapted interviews may make it possible for people with dementia to participate as research informants. Issues concerned with creating a relationship with informants with dementia and helping them to elicit their experiences are elaborated and discussed. Some suggestions are made concerning how the context can be used for this intent, and how the observations and interviews can be adapted to the informants with dementia.
Predictive Thresholds for Plague in Kazakhstan

In Kazakhstan and elsewhere in central Asia, the bacterium Yersinia pestis circulates in natural populations of gerbils, which are the source of human cases of bubonic plague. Our analysis of field data collected between 1955 and 1996 shows that plague invades, fades out, and reinvades in response to fluctuations in the abundance of its main reservoir host, the great gerbil (Rhombomys opimus). This is a rare empirical example of the two types of abundance thresholds for infectious disease—invasion and persistence— operating in a single wildlife population. We parameterized predictive models that should reduce the costs of plague surveillance in central Asia and thereby encourage its continuance.
Phosphorylation and Activation of Myosin by Rho-associated Kinase (Rho-kinase)*

The small GTPase Rho is implicated in physiological functions associated with actin-myosin filaments such as cytokinesis, cell motility, and smooth muscle contraction. We have recently identified and molecularly cloned Rho-associated serine/threonine kinase (Rho-kinase), which is activated by GTP·Rho (Matsui, T., Amano, M., Yamamoto, T., Chihara, K., Nakafuku, M., Ito, M., Nakano, T., Okawa, K., Iwamatsu, A., and Kaibuchi, K. (1996) EMBO J. 15, 2208-2216). Here we found that Rho-kinase stoichiometrically phosphorylated myosin light chain (MLC). Peptide mapping and phosphoamino acid analyses revealed that the primary phosphorylation site of MLC by Rho-kinase was Ser-19, which is the site phosphorylated by MLC kinase. Rho-kinase phosphorylated recombinant MLC, whereas it failed to phosphorylate recombinant MLC, which contained Ala substituted for both Thr-18 and Ser-19. We also found that the phosphorylation of MLC by Rho-kinase resulted in the facilitation of the actin activation of myosin ATPase. Thus, it is likely that once Rho is activated, then it can interact with Rho-kinase and activate it. The activated Rho-kinase subsequently phosphorylates MLC. This may partly account for the mechanism by which Rho regulates cytokinesis, cell motility, or smooth muscle contraction.
CHOP Is Involved in Endoplasmic Reticulum Stress-induced Apoptosis by Enhancing DR5 Expression in Human Carcinoma Cells*

It has been shown that excess stress to the endoplasmic reticulum (ER) triggers apoptosis, but the mechanisms underlying these processes remain unclear. We and others have reported previously that DR5 expression is up-regulated in thapsigargin (THG)-treated human cancer cells. Here, we provide evidence that CHOP is involved in THG up-regulation of DR5, which is a critical step for ER stress-induced apoptosis in human cancer cells. In human colon cancer HCT116 cells, knockdown of DR5 by siRNA blocked THG-induced Bax conformational change along with caspase-3 activation and cell death. Moreover, inhibition of CHOP expression attenuated DR5 up-regulation and apoptosis induced by THG, whereas ectopic expression of DR5 restored the sensitivity of CHOP siRNA-transfected cells to THG-induced apoptosis. In addition to HCT116 cells, inhibition of CHOP or DR5 induction also attenuated THG-induced cell death in other cancer cell lines including LNCaP, A2780S, and DU145, indicating that CHOP and DR5 are critical for ER stress-mediated apoptosis in human carcinoma cells. Furthermore, we identified a potential CHOP-binding site in the 5′-flanking region of the DR5 gene. Mutation of this site abrogated the enhanced reporter activity in response to THG treatment. Together, our findings suggest that CHOP regulates ER stress-induced apoptosis, at least in part, through enhancing DR5 expression in some types of human cancer cells.
Proteomic Applications of Polymeric Nanoparticles with Engineered Affinity towards Select Target

Proteins and other macromolecules of interest can be purified from crude extracts or other complex mixtures by a variety of methods. Selective precipitation is perhaps the simplest method for separating one type of macromolecule from another. Most purification methods, however, involve some form of chromatography whereby molecules in solution (mobile phase) are separated based on differences in chemical or physical interaction with a stationary material (solid phase). Gel filtration (also called size-exclusion chromatography or SEC) uses a porous resin material to separate molecules based on size (i.e., physical exclusion). In ion exchange chromatography, molecules are separated according to the strength of their overall ionic interaction with a solid phase material (i.e., nonspecific interactions).
Transcriptional Regulation of the Generic Promoter III of the Rat Prolactin Receptor Gene by C/EBPβ and Sp1*

Three promoters are operative in the rat prolactin receptor gene as follows: promoter I (PI) and II (PII) are specific for the gonads and liver, respectively, and promoter III (PIII) is common to several tissues. To investigate the mechanisms controlling the activity of promoter III, its regulatory elements and transcription factors were characterized in gonadal and non-gonadal cells. The TATA-less PIII domain was localized to the region −437 to −179 (ATG +1) containing the 5′-flanking region and part of the non-coding first exon. Within the promoter domain, a functional CAAT-box/enhancer binding protein (C/EBP) (−398) and an Sp1 element (−386), which bind C/EBPβ and Sp1/Sp3, respectively, contribute individually to promoter activation in gonadal and non-gonadal cells. However, significant redundancy was demonstrated between these elements in non-gonadal cells. Additionally, an element within the non-coding exon 1 (−338) is also required for promoter activity. Activation of PIII by the widely expressed Sp1 and C/EBPβ factors explains its common utilization in multiple tissues. Moreover, whereas the rat and mouse PIII share similar structure and function, the mouse PI lacks the functional SF-1 element and hence is inactive. These findings indicate that promoter III is of central importance in prolactin receptor gene transcription across species.