Miracle-dz/UniFAR-FTUs huggingface dataset:
Rôles de l'adiponectine à l'interface foeto-maternelle humaine au cours du premier trimestre de grossesse

L’implantation embryonnaire repose sur une synchronisation spatio-temporelle entre un placenta fonctionnel et un endometre receptif. La receptivite endometriale requiert la differenciation des cellules stromales en cellules deciduales sous l’effet des hormones ovariennes (œstrogenes et progesterone). Le placenta est un organe transitoire constitue de deux types cellulaires. Le cytotrophoblaste villeux est responsable des echanges fœtaux maternels et de la fonction endocrine du placenta. Le cytotrophoblaste extravilleux presente des proprietes invasives et assure ainsi l’ancrage du placenta dans l’endometre maternel. Un dialogue paracrine complexe entre les cellules placentaires et endometriales s’etablit au cours des premieres etapes de l’implantation.L’adiponectine est une adipokine produite majoritairement par le tissu adipeux. Elle controle le metabolisme glucido-lipidique et joue le role d’hormone insulino-sensibilisatrice. Dans de nombreux tissus, l’adiponectine exerce des effets anti-proliferatifs, pro-invasifs et pro-differenciants. L’adiponectine et ses recepteurs ADIPOR1 et ADIPOR2 sont presents a l’interface fœto-maternelle. Le placenta et l’endometre sont donc des tissus cibles de l’adiponectine.Au cours de ce travail, nous nous sommes interesses aux effets directs de l’adiponectine a l’interface foeto-maternelle au cours du premier trimestre de grossesse.Dans une premiere partie, nous avons observe que l’adiponectine exerce des effets anti-differenciants et anti-invasifs dans les cellules stromales endometriales.Dans un second temps, nous avons demontre que l’adiponectine favorise la production de glycogene dans les cellules deciduales. Inversement, l’adiponectine semble limiter l’entree du glycogene dans les cellules placentaires. Ces resultats demontrent que l’adiponectine pourrait controler la nutrition histiotrophe du foetus.Dans une derniere partie, nous avons observe que l’adiponectine diminue l’expression des transporteurs de nutriments et exerce une action pro-apoptotique dans le trophoblaste villeux. Ces derniers resultats pourraient permettre de mieux comprendre le role de l’adiponectine dans les pathologies placentaires telles que le retard de croissance intra-uterin qui se caracterise par une diminution du poids foetal et une augmentation de l’apoptose des cellules trophoblastiques.L’ensemble de ces resultats montre que l’adiponectine est un acteur cle du dialogue foeto-maternel au cours de la grossesse precoce en controlant la maturation d’un endometre fonctionnel et en regulant les echanges nutritifs transplacentaires.
LPS-binding Protein Enables Intestinal Epithelial Restitution Despite LPS Exposure

Objectives: Intestinal epithelial restitution is the first part in the process of mucosal repair after injury in the intestine. Integrity of the intestinal mucosal barrier is important as a first line of defense against bacteria and endotoxin. Necrotizing enterocolitis (NEC) is a major cause of morbidity and mortality in extremely-low-birth-weight infants, but its mechanisms are not well defined. Abnormal bacterial colonization, immature barrier function, innate immunity activation, and inflammation likely play a role. Lipopolysaccharide (LPS)-binding protein (LBP) is secreted by enterocytes in response to inflammatory stimuli and has concentration-dependent effects. At basal concentrations, LBP stimulates the inflammatory response by presenting LPS to its receptor; however, at high concentrations, LBP is able to neutralize LPS and prevent an exaggerated inflammatory response. We sought to determine how LBP would affect wound healing in an in vitro model of intestinal cell restitution and protect against intestinal injury in a rodent model of NEC. Methods: Immature intestinal epithelial cells (IEC-6) were seeded in poly-L-lysine–coated 8-chamber slides and grown to confluence. A 500-&mgr;m wound was created using a cell scraper mounted on the microscope to achieve uniform wounding. Media was replaced with media containing LPS ± LBP. Slide wells were imaged after 0, 8, and 24 hours and then fixed. Cellular restitution was evaluated via digital images captured on an inverted microscope and wound closure was determined by automated analysis. Toll-like receptor 4 (TLR4) was determined by reverse transcriptase-polymerase chain reaction after RNA isolation from wounded cells 24 hours after treatment. Results: LPS alone attenuated wound healing in immature intestinal epithelium. This attenuation is reversed by 24 hours with increasing concentrations of LBP so that wound healing is equivalent to control (P < 0.001). TLR4 was increased with LPS alone but levels returned to that of control after addition of LBP in the higher concentrations. LBP had no effect on the development of intestinal injury when given during our rodent model of NEC. Abnormal bacterial colonization and activation of innate immunity by LPS are likely involved in the pathogenesis of NEC. The attenuation of wound healing was reversed when LBP was added to LPS but only in the higher concentrations. At these same concentrations of LBP, TLR4 was decreased to that of control. Conclusions: These results indicate that LBP may be a novel therapeutic strategy to facilitate wound healing after the acute phase of NEC and other forms of intestinal injury.
The ups and downs of novel antiemetic drugs, part 2: an illustration.

ISSUE Novel antiemetic drugs block either neurokinin-1 receptors or serotonin-3 receptors. Research into the neuropharmacology of vomiting is providing insight into the ways substance P and serotonin interact within the central nervous system that could lead to additional applications of neurokinin-1 antagonists for the treatment of depression and stress.
Performance Analysis of Full-Duplex Decode-and-Forward Two-Way Relay System

This paper considers a full-duplex two-way relaying (FD-TWR) system, where two terminals transmit and receive simultaneously with the help of a full-duplex relay node. We propose the transmission scheme for decode-and-forward (DF) protocol using the differential binary phase-shift keying (DBPSK) modulation to analyse the bit error ratio (BER), which do not need the channel state information (CSI). We consider the residual self-interference (RSI) in relay and source. By using the maximum a posteriori (MAP) and maximum likelihood (ML) decoder at the relay and the source node, respectively, we obtain the symbols. The simulation results show that the full-duplex decode-and-forward two-way relaying (F2DF-TWR) system can achieve the bit error performance of the half-duplex decode-and-forward two-way relaying (H2DF-TWR) system.
Comment on “Cutting Edge: FcR-Like 6 Is an MHC Class II Receptor”

I read with interest the article by Chung et al. ([1][1]) describing how pretreatment with the serotonin reuptake inhibitor paroxetine (10 mg/d for 6 d) prevented 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine–induced neuroinflammation (increased production of reactive oxygen species and
High voltage battery charger

This paper presents the simulation and hardware result of high voltage charger. It also presents the modeling of lead-acid battery to get the dynamic charging and discharging behavior of the battery for simulation purpose. Triggering pulses for rectifier are generated using inverse cosine firing scheme. A simulation of constant voltage charging and boost cum float charging are presented and also the actual results of voltage controller are presented in this paper.
Thread-lift for facial rejuvenation: assessment of long-term results.

OBJECTIVE To evaluate the long-term success of the thread-lift procedure for facial rejuvenation. METHODS Thirty-three patients underwent a thread-lift procedure alone or in combination with other facial rejuvenation procedures to the brow, midface, jowl, and neck. Ten patients underwent thread-lifts only, and 23 had thread-lifts with other procedures. Ten additional patients having had non-thread-lift rejuvenation procedures, including lipotransfer, chemical peels, and rhytidectomies, were randomly designated as controls. The mean follow-up period was 21 months (range, 12-31 months). Photodocumentation was obtained at each visit. Long-term aesthetic results were evaluated by 4 independent, blinded, and board-certified facial plastic surgeons. Each result was graded on a scale of 0 to 3, with 0 indicating no change; 1, minimal improvement; 2, moderate improvement; and 3, considerable improvement. The population was divided into 3 groups for comparison. Two-tailed t test (P = .05) was used for statistical analysis of aesthetic outcomes. RESULTS Although aesthetic improvement was noted in all groups at 1 month, measurable results persisted to the end of the study for all but the group that underwent the thread-lift procedure only. Aesthetic improvement scores of the non-thread-lift control group were better than the group that underwent thread-lift only. Similarly, when the thread-lift was combined with other procedures, scores were better than when thread-lift was used alone. Statistical significance was demonstrated in both of these comparisons (P < .01). CONCLUSIONS The thread-lift provides only limited short-term improvement that may be largely attributed to postprocedural edema and inflammation. Our results objectively demonstrate the poor long-term sustainability of the thread-lift procedure. Given these findings, as well as the measurable risk of adverse events and patient discomfort, we cannot justify further use of this procedure for facial rejuvenation.
RNase K: one less letter in the alphabet soup

RNase E, encoded by the me locus, is believed to be the key endoribonuclease that controls mRNA decay in Escherichia coil RNase K has been defined as the enzyme that regulates the stability of the ompA mRNA with respect to growth rate. Recent data suggest that the specificity of RNase E is sufficient to explain the cleavage activity attributed to RNase K. Some years ago we discovered that the stability of the E. coil ompA mRNA, coding for the outer membrane protein _A, is, in contrast to many other E. coil transcripts, growthrate regulated (Nilsson et al., 1984, EMBO J 7: 22692275). Subsequently we identified site-specific endoribonucleolytic cleavages in the 5' untranslated region (UTR) of ompA mRNA that seem to regulate the stability of this transcript (Melefors and von Gabain, 1988, Cell 52: 893901). We partially purified an endoribonuclease (5560kDa) that introduces the growth-rate regulated cleavages in the ompA mRNA, and we named it RNase K (Lundberg et aL, 1990, EMBO J 9: 2731-2741). The cleavages in the ompA mRNA were found to be impaired in vivo in a conditional, ares temperature-sensitive (ts), mutant strain that shows a deficiency in bulk mRNA decay at the non-permissive temperature (Ono and Kuwano, 1979, J Mo/ Biol 129: 343-357; Lundberg et al., 1990, ibid.). Since the ams gene affected bulk mRNA decay and concomitantly ompA mRNA 5' processing and degradation, we concluded that RNase K was an important component in the control of bulk mRNA decay, even though the exact relationship between the ams gene and RNase K was not determined (i.e. whether RNase K was the ares gene product or not) (Lundberg, eta/., 1990, ibid.). RNase E, which cleaves the 9S ribosomal RNA processing intermediate to form p5S RNA, was identified by isolation of a different thermosensitive mutant allele, rne3071(ts) (Apirion, 1978, Genetics 90: 659-671). Bulk mRNA decay in an rne(ts) mutant strain was initially shown to be unaffected at non-permissive temperatures (Apirion and Gitelman, 1980, J Mol Bio/177: 339-343). This notion, and experiments with extracts from an rne(ts) mutant strain that did not show a reduction in cleavage of the 5'-UTR of the ompA mRNA in vitro at nonpermissive temperatures (Nilsson et aL, 1988, EMBO J 7: 2269-2275; Melefors et aL, 1993, In Control of Messenger RNA Stability, Brawerman and Belasco (eds), Academic Press, pp. 53-70), led us to conclude that RNase K and
Visual scaffolding for encouraging online participation

The purpose of this study is to develop a visual scaffolding for motivating learners participate online learning actively. Two types of visual scaffolding were developed and the visual scaffolding is represented individual participation, group participation, and the interaction among groups. In order to investigate the effects of the visual scaffolding on online participation, the quasi-experimental study was conducted with 39 undergraduate students. The results showed that the visual scaffolding enhanced learner's online participation.
Power grip strength as a function of tool handle orientation and location

Thirty male volunteers participated in a study evaluating the effect of workspace envelope (work height and reach distance) and handle orientation on grip force capacity. Maximum voluntary power grip exertions were recorded using instrumented tool handles under three conditions: a pistol grip tool handle oriented horizontally and vertically and a right angle tool handle oriented horizontally. Significant main effects of handle height and reach location on normalized grip force capacity were observed with the horizontally oriented pistol grip and right angle handles, whereas only an interaction effect was observed with the vertically oriented pistol grip handle. Comparison of results to scores produced with a job assessment tool (RULA) is included as an appendix. The proposed methodology can provide information useful to job, workstation or tool design directed toward best accommodating the physical capacities of workers performing hand tool tasks.
Recombinant human insulin-like growth factor-1 induces an anabolic response in malnourished CAPD patients.

BACKGROUND Insulin-like growth factor-1 (IGF-1) is an anabolic hormone that mediates most of the growth effects of growth hormone. This study tested the hypothesis that recombinant human IGF-1 (rhIGF-1) will induce an anabolic response in malnourished patients undergoing continuous ambulatory peritoneal dialysis (CAPD). METHODS Six CAPD patients with protein-energy malnutrition underwent nitrogen balance studies in a clinical research center for 35 days each. Throughout the study, patients were maintained on their same CAPD regimen prior to hospitalization, and were fed a constant protein and energy intake that was similar to their diet prior to hospitalization. The first 15 hospital days were a baseline period; during the subsequent 20-day period, patients were given subcutaneous injections of rhIGF-1 (100 microg/kg/12 h), except for one patient who received 50 microg/kg/12 h for the first five days, followed by 100 microg/kg/12 h for the following 15 days. RESULTS During the treatment with rhIGF-1, serum IGF-1 increased by about 100% (P = 0.03), and nitrogen balance became strongly positive (+2.0 g/day, P = 0.015 vs. baseline). This anabolic effect was observed within hours after commencing the rhIGF-1 treatment and was largely caused by a 20% decrease in peritoneal dialysate effluent nitrogen. There was a proportionate reduction in urine nitrogen and serum urea nitrogen. This decrease in nitrogen output was sustained during the entire 20 day of treatment with rhIGF-1. Serum phosphorus decreased significantly during the first several days of rhIGF-1 treatment, whereas serum calcium increased significantly during the rhIGF-1 treatment. Serum potassium and albumin did not change during the rhIGF-1 injections. There was no change in body weight and body composition, as assessed by anthropometry during the baseline or treatment phases of the study. Some patients exhibited minor possible adverse events that included a reduction in blood pressure and transient tachycardia. CONCLUSION Injections of rhIGF-1 induce a strong and sustained anabolic effect, as indicated by a positive nitrogen balance in CAPD patients with protein-energy malnutrition. rhIGF-1 administration may be an effective method for treating malnutrition in maintenance dialysis patients.
THE COMING AGE OF CALM TECHNOLOGY[1]

In the past fifty years of computation there have been two great trends in this relationship: the mainframe relationship, and the PC relationship. Today the Internet is carrying us through an era of widespread distributed computing towards the relationship of ubiquitous computing, characterized by deeply imbedding computation in the world. Ubiquitous computing will require a new approach to fitting technology to our lives, an approach we call "calm technology".
Brain cysts associated with mutation in the Aristaless related homeobox gene, ARX

The novel Aristaless related homeobox gene, ARX, is widely expressed in the brain and is thought to play a key role in the regulation of brain development. Neurological phenotypes caused by ARX mutations have recently started to unfold. We describe a 72 year old man with X-linked mental retardation due to a 24 bp duplication mutation in exon 2 of the ARX gene. Cerebral MRI showed bilateral cystic-like cavities in both the cerebral and cerebellar hemispheres. No retraction or expansion in neighbouring parenchyma was observed, there was no history of acute neurological impairment, and no risk factors for cerebrovascular disease were found. The lesions appeared to be congenital and represented benign developmental cysts, possibly caused by the ARX mutation.
A comparison between Ki‐67 antibody reactivity and other pathological variables in breast carcinoma

Summary Methods of assessing tumor proliferation rates include mitosis counting, flow cytometry and thymidine labelling. While the former is inaccurate and poorly reproducible, the latter methods are time consuming and expensive to perform. Ki‐67 is a monoclonal mouse antibody which has been shown to react with a nuclear antigen in proliferating cells. Frozen sections from 75 specimens of breast carcinoma were immunostained with this antibody using an immunoperoxidase technique. The percentage of tumor cells stained, the Ki‐67 score, was then compared with a number of pathological and clinical variables in the patients concerned. A positive correlation was seen between the Ki‐67 score and mitotic rate (r = 0.71); and a negative correlation was seen between Ki‐67 score and estrogen receptor status (r = ‐0.4). Ki‐67 immunostaining may represent a cheap and reproducible method of assessing proliferation rates of breast carcinomas which is applicable in routine laboratories. Further prospective studies are being undertaken to assess its contribution to prognosis.
Involvement of β1 integrin in microglial chemotaxis and proliferation on fibronectin: Different regulations by ADP through PKA

Microglia are immune cells in the brain; their activation, migration, and proliferation have pivotal roles in brain injuries and diseases. Microglia are known to attach firmly to fibronectin, the upregulation of which is associated with several pathological conditions in the CNS, through β1 integrin and become activated. Extracellular nucleotides can serve as potent signaling molecules. Recently, ATP and ADP were revealed to possess chemoattractive properties to microglia via Gi‐coupled P2Y receptors. In the present study, we report that the ADP‐induced chemotaxis of microglia is mediated by P2Y12/13 receptors and is β1 integrin‐dependent in the presence of fibronectin. Signals from P2Y12/13 receptors also cause β1 integrin translocation to the membrane ruffle regions, but this redistribution was lost when the intracellular cyclic AMP (cAMP) was increased by forskolin or dibutyryl cAMP. This inhibitory effect of cAMP‐elevating agents did not appear when microglia were co‐incubated with a protein kinase A (PKA) inhibitor, KT‐5720, suggesting that PKA is a negative regulator of the β1 integrin translocation. We also show that the engagement of β1 integrin enhanced microglial proliferation. Signals from P2Y12/13 receptors attenuated the proliferation, whereas ADP itself had no effect on microglial growth. Furthermore, β1 integrin‐induced proliferation is positively regulated by the cAMP‐dependent PKA. Together, these results indicate the involvement of β1 integrin in microglial proliferation and chemotaxis, both of which have clinical importance. The data also suggest that PKA is inversely involved in these two cellular functions. © 2005 Wiley‐Liss, Inc.
Nondestructive inspection of through silicon via depth using scanning acoustic microscopy

In this paper, an innovative application of an existing nondestructive inspection, i.e, time-of-flight mode of scanning acoustic microscopy (SAM), was proposed for the measurement of through silicon via (TSV) depth. A feasibility study was performed on a dummy wafer with various sizes of TSVs to evaluate this method. From the image reconstruction of B-scan SAM, the depth of TSVs can be clearly identified. In addition, cross-section inspections were performed to verify this nondestructive inspection method. It was found that, for TSV sizes larger than φ30 μm, the depth measured by the ultrasonic scanning method could be very close to the results of cross-section inspection (less than 5% error). The current results are considered very encouraging and the proposed nondestructive inspection method should have very good potential for the high throughput and low cost in-line measurement of TSV depth during the TSV forming process.
Long noncoding RNA SNHG12 promotes the proliferation, migration, and invasion of trophoblast cells by regulating the epithelial–mesenchymal transition and cell cycle

Objective The deficient placental blood perfusion caused by the attenuated infiltration of trophoblast cells is a key factor in the occurrence of preeclampsia (PE). Furthermore, the long noncoding (lnc)RNA SNHG12 (small nucleolar RNA host gene 12) can promote the proliferation and metastasis of multiple tumor cells. However, whether lncRNA SNHG12 affects proliferation and metastasis of trophoblast cells is unclear. Methods We examined the level of lncRNA SNHG12 in plasma and placenta of patients with PE and constructed trophoblast cells with overexpressed or knocked down SNHG12. CCK-8, wound healing, and Transwell assays were used to detect alterations in proliferation, migration, and invasion of trophoblast cells. Western blotting was used to detect proteins related to the epithelial–mesenchymal transition (EMT), and cell cycle assays clarified cell cycle distribution. Results LncRNA SNHG12 promoted the proliferation, migration, and invasion of trophoblast cells. The expression of matrix metalloproteinase-2 (MMP-2) and MMP-9, β-catenin, and vimentin were positively correlated with SNHG12, and expression of E-cadherin was negatively correlated with SNHG12. SNHG12 also promoted the transition of trophoblast cells from G0/G1 to S phase. Conclusion Overall, lncRNA SNHG12 promoted the migration and invasion of trophoblast cells by inducing the progression of EMT.
Molecular characterization of SSS139, a new satellite DNA family in sibling species of the Drosophila buzzatii cluster

We characterized sequences of a novel SSS139 RsaI satellite DNA family in Drosophila gouveai and Drosophila seriema, two members of the Drosophila buzzatii cluster (D. repleta group). The sequences were AT-rich (69%) with a monomer unit length of about 139 bp and contained two direct subrepeats of 14 bp and 16 bp, suggesting that it might have originated by the duplication of smaller sequences. Southern and dot-blot hybridization analyses also detected SSS139 in other Drosophila buzzatii cluster species (D. koepferae, D. antonietae, D. borborema and D. serido) but not in D. buzzatii. These results agree with the marginal phylogenetic position of D. buzzatii within the D. buzzatii cluster.