Miracle-dz/UniFAR-FTUs huggingface dataset:
Progressive fibrosing interstitial lung disease: a clinical cohort (the PROGRESS study)

In patients with chronic fibrosing interstitial lung disease (ILD), a progressive fibrosing phenotype (PF-ILD) may develop, but information on the frequency and characteristics of this population outside clinical trials is lacking. We assessed the characteristics and outcomes of patients with PF-ILD other than idiopathic pulmonary fibrosis (IPF) in a real-world, single-centre clinical cohort. The files of all consecutive adult patients with fibrosing ILD (2010–2017) were examined retrospectively for pre-defined criteria of ≥10% fibrosis on high-resolution computed tomography and progressive disease during overlapping windows of 2 years. Baseline was defined as the date disease progression was identified. Patients receiving nintedanib or pirfenidone were censored from survival and progression analyses. In total, 1395 patients were screened; 617 had ILD other than IPF or combined pulmonary fibrosis and emphysema, and 168 had progressive fibrosing phenotypes. In 165 evaluable patients, median age was 61 years; 57% were female. Baseline mean forced vital capacity (FVC) was 74±22% predicted. Median duration of follow-up was 46.2 months. Annualised FVC decline during the first year was estimated at 136±328 mL using a linear mixed model. Overall survival was 83% at 3 years and 72% at 5 years. Using multivariate Cox regression analysis, mortality was significantly associated with relative FVC decline ≥10% in the previous 24 months (p<0.05), age ≥50 years (p<0.01) and diagnosis subgroup (p<0.01). In this cohort of patients with PF-ILD not receiving antifibrotic therapy, the disease followed a course characterised by continued decline in lung function, which predicted mortality. In a real-world clinical cohort (PROGRESS), progressive fibrosing interstitial lung disease was characterised by continued lung function decline. Lung function decline, age and underlying diagnosis subgroup predicted mortality. https://bit.ly/2EB3OpF
A Perspective on Implementing Movement Sonification to Influence Movement (and Eventually Cognitive) Creativity

Creativity represents an important feature in a variety of daily-life and domain-specific contexts. Recent evidence indicates that physical movement serves as a key resource for exploring and generating task-relevant creative ideas, supporting the embodied perspective on creative cognition. An intuitive link between movement and creative cognition is movement creativity. The process of exploring the movement solutions an environment offers (i.e., affordances) and exploiting novel, functional, and creative movements may translate to and improve how individuals explore and generate novel ideas. Opening perception to the variety of affordances (“conventional” and novel) an environment offers drives creative movement. Teachers and coaches can promote this process by designing a learning environment that invites performers to consider and utilize novel movement solutions. In this article, we present a rationale for using movement sonification to promote creative movement. Movement sonification consists of mapping a movement parameter into sound, with a sound being triggered or changing according to how movement unfolds. We argue that movement sonification can facilitate the emergence of creative movement via enhancing perception of currently performed movements and invite performers to utilize novel affordances, and emphasizing information for regulating subsequent creative actions. We exemplify this concept in a creative dance intervention for children during physical education classes. In conclusion, we contend that learning to explore original dance sequences using movement sonification may provide a meaningful link between creative movement and creative cognition. Children may use their minds and bodies as tools for creative thinking and exploration, such as shaping letters with their bodies.
Plasma-Based Longitudinal Evaluation of ESR1 Epigenetic Status in Hormone Receptor-Positive HER2-Negative Metastatic Breast Cancer

Background Endocrine therapy (ET) is the mainstay of treatment for hormone receptor-positive human epidermal growth factor receptor 2 (HER2)-negative metastatic breast cancer; however, adaptive mechanisms emerge in about 25–30% of cases through alterations in the estrogen receptor ligand-binding domain, with a consequent ligand-independent estrogen receptor activity. Epigenetic-mediated events are less known and potentially involved in alternative mechanisms of resistance. The aim of this study was to test the feasibility of estrogen receptor 1 (ESR1) epigenetic characterization through liquid biopsy and to show its potential longitudinal application for an early ET sensitivity assessment. Methods A cohort of 49 women with hormone receptor-positive HER2-negative MBC was prospectively enrolled and characterized through circulating tumor DNA using methylation-specific droplet digital PCR (MS-ddPCR) before treatment start (BL) and after 3 months concomitantly with computed tomography (CT) scan restaging (EV1). ESR1 epigenetic status was defined by assessing the methylation of its main promoters (promA and promB). The most established cell-free tumor DNA (ctDNA) factors associated with ET resistance [ESR1 and phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) mutations] were assessed through next-generation sequencing. Associations were tested through Mann–Whitney U test, matched pairs variations through Wilcoxon signed rank test, and survival was analyzed by log-rank test. Results The ET backbone was mainly based on aromatase inhibitors (AIs) (70.83%) in association with CDK4/6 inhibitors (93.75%). Significantly lower promA levels at baseline were observed in patients with liver metastases (P = 0.0212) and in patients with ESR1 mutations (P = 0.0091). No significant impact on PFS was observed for promA (P = 0.3777) and promB (P = 0.7455) dichotomized at the median while a ≥2-fold increase in promB or in either promA or promB at EV1 resulted in a significantly worse prognosis (respectively P = 0.0189, P = 0.0294). A significant increase at EV1 was observed for promB among patients with PIK3CA mutation (P = 0.0173). A trend was observed for promB in ESR1 wild-type patients and for promA in the ESR1 mutant subgroup. Conclusion The study proofed the concept of an epigenetic characterization strategy based on ctDNA and is capable of being integrated in the current clinical workflow to give useful insights on treatment sensitivity.
What’s in Your Gifted Education Online Teacher Professional Development? Incorporating Theory- and Practice-Based Elements of Instructional Learning Design

This article examined six theory- and practice-based elements of instructional learning design in online teacher professional development (oTPD), how these elements were implemented into Edinger’s PACKaGE model of gifted education oTPD, and how teachers evaluated each element. Elements were based on Berge’s (1995) instructor roles model theory and gifted education research. Each element was evaluated by teachers (N = 184) who completed oTPD designed from the PACKaGE model. Self-report survey findings suggest that teachers considered most elements, such as asynchronous discussion board and article review assignments, to be useful to a great extent to their gifted education learning and pedagogy. However, teachers reported less usefulness for the online group project element. This finding directly contrasts with researchers’ suggestions for oTPD. These findings may encourage teachers to choose theory- and research-based oTPD. PD designers can investigate and implement oTPD elements that satisfy instructional design model needs for optimal teacher learning and gifted education pedagogy.
Anterograde Axonal Transport in Neuronal Homeostasis and Disease

Neurons are highly polarized cells with an elongated axon that extends far away from the cell body. To maintain their homeostasis, neurons rely extensively on axonal transport of membranous organelles and other molecular complexes. Axonal transport allows for spatio-temporal activation and modulation of numerous molecular cascades, thus playing a central role in the establishment of neuronal polarity, axonal growth and stabilization, and synapses formation. Anterograde and retrograde axonal transport are supported by various molecular motors, such as kinesins and dynein, and a complex microtubule network. In this review article, we will primarily discuss the molecular mechanisms underlying anterograde axonal transport and its role in neuronal development and maturation, including the establishment of functional synaptic connections. We will then provide an overview of the molecular and cellular perturbations that affect axonal transport and are often associated with axonal degeneration. Lastly, we will relate our current understanding of the role of axonal trafficking concerning anterograde trafficking of mRNA and its involvement in the maintenance of the axonal compartment and disease.
Control Effect of the Pine Wood Nematode Disease Transmitted by Monochamus alternatus through Releasing Parasitoid Dastarcus helophoroides(Fairmaire) and Using Bait-trees

The pine wood nematode,Bursaphelenchus xylophilus,is an important invasive forest pest in China;and Japanese pine sawyer,Monochamus alternatus is the major vector insect.To explore sustainable management of the pine wilt disease,bait-trees were set in the forests to lure adults of M.alternatus for oviposition,which would be eradicated before adult emergence in the next year.Meanwhile,parasitoid Dastarcus helophoroides(Fairmaire) was released in sample stands.Then the spread chain of pine wood nematode would be cut off by the control measurements mentioned above.Results showed that the number of dead and weak trees decreased significantly in the plots with bait-trees and release of natural enemies compared with the control area.The control efficiency was 71.27% and 90.25% in bait-tree plot and natural enemy release plot,respectively.The trees infested with pine wood nematode and the population density of M.alternatus also reduced significantly in the two treatment plots.The parasitism rates of M.alternatus were 38.34% and 3.92% in the parasitoid release area and the control area, respectively.The indoor tests showed that parasitism rates were 12.5%—40% when the natural enemy was released at the ratio of 1:1—4:1(natural enemy to beetle),there were no significant differences in parasitism rates among different releasing proportions.The parasitism rate increased significantly when a log was released with 10 D. helophoroides adults(19.45%) than with 4 adults(4.11%). These findings indicate that natural enemies play an important role against the population of M.alternatus in forests,thus preventing pine sawyer to spread the pine wood nematode disease.
Isotopic Fractionation Associated With Sulfate Import and Activation by Desulfovibrio vulgaris str. Hildenborough

The use of stable isotopes to trace biogeochemical sulfur cycling relies on an understanding of how isotopic fractionation is imposed by metabolic networks. We investigated the effects of the first two enzymatic steps in the dissimilatory sulfate reduction (DSR) network – sulfate permease and sulfate adenylyl transferase (Sat) – on the sulfur and oxygen isotopic composition of residual sulfate. Mutant strains of Desulfovibrio vulgaris str. Hildenborough (DvH) with perturbed expression of these enzymes were grown in batch culture, with a subset grown in continuous culture, to examine the impact of these enzymatic steps on growth rate, cell specific sulfate reduction rate and isotopic fractionations in comparison to the wild type strain. Deletion of several permease genes resulted in only small (∼1‰) changes in sulfur isotope fractionation, a difference that approaches the uncertainties of the measurement. Mutants that perturb Sat expression show higher fractionations than the wild type strain. This increase probably relates to an increased material flux between sulfate and APS, allowing an increase in the expressed fractionation of rate-limiting APS reductase. This work illustrates that flux through the initial steps of the DSR pathway can affect the fractionation imposed by the overall pathway, even though these steps are themselves likely to impose only small fractionations.
Effects of different selenium sources on growth performance,carcass composition and meat quality in broilers.

A total of 600 Lingnan yellow broilers were selected to study the effects of different selenium sources on growth performance,carcass composition and meat quality.The results showed: in the group with basal diet added 0.15 mg/kg Se level of sodium selenite,methionine selenium,yeast selenium respectively,birds weight gain were increased by 16.08%(P0.01) and 17.86%(P0.01) respectively,feed gain ratio were improved 15.26%(P0.01) and 16.19%(P0.01),and there was significant differences between organic Se and inorganic Se.Compared with the basal diet group,meat red color degree in group of methionine selenium and yeast selenium were increased 20.84%(P0.01), 10.76%(P0.05),drip loss were decreased 21.32%(P0.01) and 14.23%(P0.05) respectively,and there was significant differences between organic Se and inorganic Se.The results also indicated that different selenium sources did not affect carcass composition of the broilers.
Genetic Fusions of Globular Proteins to the Subunit of the Escherichia coli ATP Synthase IMPLICATIONS FOR IN VIVO ROTATIONAL CATALYSIS AND SUBUNIT FUNCTION*

The rotational mechanism of ATP synthase was investigated by fusing three proteins from Escherichia coli, the 12-kDa soluble cytochrome b562, the 20-kDa flavodoxin, and the 28-kDa flavodoxin reductase, to the C terminus of the subunit of the enzyme. According to the concept of rotational catalysis, because is part of the rotor a large domain added at this site should sterically clash with the second stalk, blocking rotation and fully inhibiting the enzyme. E. coli cells expressing the cytochrome b562 fusion in place of wild-type grew using acetate as the energy source, indicating their capacity for oxidative phosphorylation. Cells expressing the larger flavodoxin or flavodoxin reductase fusions failed to grow on acetate. Immunoblot analysis showed that the fusion proteins were stable in the cells and that they had no effect on enzyme assembly. These results provide initial evidence supporting rotational catalysis in vivo. In membrane vesicles, the cytochrome b562 fusion caused an increase in the apparent ATPase activity but a minor decrease in proton pumping. Vesicles bearing ATP synthase containing the larger fusion proteins showed reduced but significant levels of ATPase activity that was sensitive to inhibition by dicyclohexylcarbodiimide (DCCD) but no proton pumping. Thus, all fusions to generated an uncoupled component of ATPase activity. These results imply that a function of the C terminus of in F1F0 is to increase the efficiency of the enzyme by specifically preventing the uncoupled hydrolysis of ATP. Given the sensitivity to DCCD, this uncoupled ATP hydrolysis may arise from rotational steps of in the inappropriate direction after ATP is bound at the catalytic site. It is proposed that the C-terminal domain of functions to ensure that rotation occurs only in the direction of ATP synthesis when ADP is bound and only in the direction of hydrolysis when ATP is bound.
The rapid progress in characterization of mammalian genome sequence will facilitate the use of mouse mutants for understanding gene function, and for investigating how perturbations of biological pathways can result in disease

The rapid progress in characterization of mammalian genome sequence will facilitate the use of mouse mutants for understanding gene function, and for investigating how perturbations of biological pathways can result in disease. Polycystic kidney disease (PKD) represents a major cause of human morbidity and mortality. Although PKD1 and PKD2, the genes responsible for the great majority of human autosomal dominant PKD, have both been cloned, the biological basis of this disorder is not understood (American PKD1 Consortium, 1995; European Polycystic Kidney Disease Consortium, 1994; International PKD Consortium, 1995). Cystic degeneration in PKD is marked by the failure to maintain differentiated epithelial cell form and function; this is likely to involve multiple cell signaling pathways and cell structures, including those required for cell adhesion, maintainance of the cytoskeleton and epithelial cell polarity. Genetic analysis of epithelial cyst formation in animal models will facilitate the molecular characterization of novel genes required for normal epithelial function and will contribute to understanding the cellular pathology of cystic disease. The juvenile cystic kidney (jck) mutation is a model of autosomal recessive PKD (Atala et al., 1993). The jck mutation was initially mapped to a 1.5 cM interval on mouse chromosome 11 (Iakoubova et al., 1995), and modifying loci affecting progression of PKD in this system have been identified (Iakoubova et al., 1999; Kuida and Beier, 2000). We describe the positional cloning of the gene mutated in jck, and the characterization of this defect using both in vitro analysis and a test of gene function in zebrafish embryos.
DEV110437 4395..4405

UNC-6/Netrin is a conserved axon guidance cue that canmediate both attraction and repulsion. We previously discovered that attractive UNC-40/DCC receptor signaling stimulates growth cone filopodial protrusion and that repulsive UNC-40–UNC-5 heterodimers inhibit filopodial protrusion in C. elegans. Here, we identify cytoplasmic signaling molecules required for UNC-6-mediated inhibition of filopodial protrusion involved in axon repulsion. We show that the Rac-like GTPases CED-10 and MIG-2, the Rac GTP exchange factor UNC-73/Trio, UNC-44/Ankyrin and UNC-33/CRMP act in inhibitory UNC-6 signaling. These molecules were required for the normal limitation of filopodial protrusion in developing growth cones and for inhibition of growth cone filopodial protrusion caused by activated MYR::UNC-40 and MYR::UNC-5 receptor signaling. Epistasis studies using activated CED-10 and MIG-2 indicated that UNC-44 and UNC33 act downstream of the Rac-like GTPases in filopodial inhibition. UNC-73, UNC-33 and UNC-44 did not affect the accumulation of fulllength UNC-5::GFP and UNC-40::GFP in growth cones, consistent with a model in which UNC-73, UNC-33 and UNC-44 influence cytoskeletal function during growth cone filopodial inhibition.
Plasmid-Mediated 1-Lactamase with Two Molecular Variants

KiebsieUla pneumoniae BA32, a clinical isolate from Buenos Aires, Argentina, was found to produce a plasmid-encoded ,B-lactamase (FOX-1) which conferred resistance to broad-spectrum cephalosporins and cephamycins. Resistance could be transferred by conjugation or transformation into Escherichia coli K-12 via a 48.5-kb plasmid (pGLKI) that produced two FOX-1 molecular variants with isoelectric points of 6.8 and 7.2 and apparent molecular sizes of 37 and 35 kDa, respectively. The kinetic study revealed that the two variants had very similar substrate and inhibition profiles. These values resemble those of chromosomally mediated class C (group 1) cephalosporinases. The structural gene of FOX-1 (blaFOXl) was cloned into a 2,270-bp PstI-PstI fragment and was expressed in E. coli TGI. The deduced 382-amino-acid sequence ofFOX-1 exhibited
Study on the Antioxidant Activity of Hydrosol from Chestnut Flower

The antioxidant activity of hydrosol from chestnut flower was investigated by determining DPPH radical scavenging ability,reducing power,hydroxyl radical scavenging ability and nitrite scavenging ability,using commercially available toilet water repellents and Vc as the controls. The results showed that when the concentration of chestnut flower hydrosol was 0. 5 mg / m L,the scavenging ability of DPPH radical was the highest and scavenging rate was 80. 64%,higher than that of Liu Shen( 70. 21%) and Long Li Qi( 60. 14%) toilet water and less than that of Vc( 90. 04%),the value of IC50 was 0. 25 mg / m L. When the concentrations of chestnut flower hydrosol were 0. 1 mg / m L and 4 mg / m L,the scavenging rates of hydroxyl radical and nitrite were 74. 03% and 84. 77%,respectively,which were the highest in the same levels of all samples. The reducing power of chestnut flower hydrosol was the highest at the concentration of 1.5 mg / m L,lower than Vc and higher than commercial toilet waters. The results confirmed that chestnut flower hydrosol exhibited the excellent antioxidant ability. It provided basis for the further development and utilization of chestnut flower hydrosol.
N-Sulfonated Monocyclic 3-Lactam, in Rats and Dogs

Rats and dogs were given a single 20-mg/kg dose of [14Clcarumonam intramuscularly or intravenously. In rats, the level in plasma of [14C]carumonam administered intramuscularly peaked (29.1 ,ug/ml) 15 min after dosing and then declined with an apparent elimination half-life of 16.2 min. Intramuscular injection of ["4C]carumonam to dogs gave a peak level (36.8 ,ug/ml) in plasma at 20 min and an apparent elimination half-life of 51.7 min. After administration of the intravenous dose, apparent elimination half-lives were 13.1 and 52.7 min in rats and dogs, respectively. In both animals, the radioactivity in plasma was made up largely (>80%) of unchanged carumonam, which was protein bound to only a small extent. In rats given ["4C]carumonam intramuscularly, radioactivity was distributed widely in tissues, with relatively high concentrations in the kidney and liver. The radioactivity concentration in the rat fetus was relatively low, as
EVIDENCE FOR ECDYSTEROID FEEDBACK ON RELEASE OF MOLT INHIBITING HORMONE FROM CRAB EYESTALK GANGLIA

The content and release of molt-inhibiting hormone activity (MIH) in isolated eyestalk ganglia of crabs (Cancer antennarius S.) were measured in vitro as a function of exposure in vivo to elevated or reduced hemolymph ecdysteroid levels. Ecdysteroid titers of intermolt crabs injected with 20-hydroxyecdysone (two 45 µg injections/24 h) rose 8- to 10-fold; MIH released from subsequently isolated ganglia was significantly less than that released from ganglia of saline-injected controls, while MIH content of ganglia from treated crabs was increased. The hemolymph ecdysteroid level of intermolt crabs was low (6 ng/ml) and was further reduced by 40% 6 days after Y-organectomy. MIH release from ganglia of both Y-organectomized and sham-operated control crabs was high, similar to that of unoperated controls, but MIH content of ganglia from both Y-organectomized and sham groups was significantly reduced relative to controls. The results indicate a negative feedback regulation of MIH release but not synthesis by ec.