Miracle-dz/UniFAR-FTUs huggingface dataset:
The molecular genetics of cellular oncogenes.

DEFINITIONS OF CELLULAR ONCOGENES 555 c-one's: The Proto-Oncogenic Progenitors of Retroviral Oncogenes . 555 Identification of Cellular Oncogenes by Functional Tests for Dominant Mutations 558 Using Genetic Rearrangements to Identify Oncogenes . 562 Proviral insertions can activate expression oj cellular proto-oncogenes 563 Oncogenes at translocation breakpoints. 566 Oncogenes in amplified DNA . 567 MOLECULAR PROFILES OF ACTIVATED ONCOGENES 570 Base Substitutions in Mutant ras Alleles . 570 Insertionally Mutated c-myc Loci. 574 Locations oj proviruses 574 Additional mutations in proviraUy mutated loci . 576 Characteristics of Other Proviral Insertion Mutations in Proto-Oncogenes 578 Structural and Functional Properties of Translocation Breakpoints near Cellular Oncogenes . 581 General Jeatures oj c-myc; immunoglobulin gene translocations . 581 Specific examples of c-myc; Ig translocations in PC and BL lines . 583 The Phi chromosome . 586 Other translocations and rearrangements in B cell tumor lines . 587 Structural Features oj Amplified Oncogenes . 587 FROM c-onc TO v-onc: COLLUSION OF MUTATIONAL MECHANISMS. 588 The Favored Model Jar Transduction of Oncogenes . 590 The Functional Significance of Diff erences Between c-onc' s and v-onc' s 592 INACTIVATION OF INTEGRATED v-onc GENES: A SAMPLER OF MUTATIONAL MECHANISMS 593
Hemocultivos y sepsis por cateterismo intravascular en los Servicios Críticos de Atención al Grave

Se estudiaron 164 hemocultivos de pacientes con sospecha de sepsis de causa no precisada ingresados en los Servicios Criticos de Atencion al Grave [Unidad de Cuidados Intensivos (UCI) y Unidad de Cuidados Intermedios (UCIM)] del Hospital “Dr Salvador Allende” del municipio Cerro, Ciudad de La Habana, para determinar mediante tecnica bacteriologica cuantitativa de hemocultivos, el origen de sepsis por cateterismo intravascular. Se tomaron 2 muestras de hemocultivos, una obtenida mediante venipuncion periferica y otra, mediante cateter implantado en estos pacientes. Se procesaron las muestras segun tecnica bacteriologica cuantitativa. Se preciso que en 48,39 % de los casos, la sepsis presentada por el paciente era por el cateter intravenoso y en el 51,61 % el foco de infeccion se encontraba en otra localizacion. Los microorganismos mas frecuentes aislados fueron 10 cepas de S. aureus (32,26 %); 7 cepas de estafilococos coagulasa negativos (22,58 %) y 6 cepas de bacilos no fermentadores (19,35 %).
Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype

Two outbreaks of hemorrhagic colitis, a newly recognized syndrome characterized by bloody diarrhea, severe abdominal pain, and little or no fever, occurred in 1982. No previously recognized pathogens were recovered from stool specimens from persons in either outbreak. However, a rare E. coli serotype, O157:H7, was isolated from 9 of 20 cases and from no controls. It was also recovered from a meat patty from the implicated lot eaten by persons in one outbreak. No recovery of this organism was made from stools collected 7 or more days after onset of illness; whereas 9 of 12 culture-positive stools had been collected within 4 days of onset of illness. The isolate was not invasive or toxigenic by standard tests, and all strains has a unique biotype. Plasmid profile analysis indicates that all outbreak-associated E. coli O157:H7 isolates are closely related. These results suggest that E. coli O157:H7 was the causative agent of illness in the two outbreaks.
Relationships of outdoor and indoor ultrafine particles at residences downwind of a major international border crossing in Buffalo, NY.

UNLABELLED During winter 2006, indoor and outdoor ultrafine particle (UFP) size distribution measurements for particles with diameters from 5.6 to 165 nm were taken at five homes in a neighborhood directly adjacent to the Peace Bridge Complex (PBC), a major international border crossing connecting Buffalo, New York to Fort Erie, Ontario. Monitoring with 1-s time resolution was conducted for several hours at each home. Participants were instructed to keep all external windows and doors closed and to refrain from cooking, smoking, or other activity that may result in elevating the indoor UFP number concentration. Although the construction and age for the homes were similar, indoor-to-outdoor comparisons indicate that particle infiltration rates varied substantially. Overall, particle concentrations indoors were lower and less variable than particle concentrations outdoors, with average indoor-outdoor ratios ranging from 0.1 to 0.5 (mean 0.34) for particles between 5.6 and 165 nm in diameter. With no indoor sources, the average indoor-outdoor ratios were lowest (0.2) for 20-nm particles, higher (0.3) for particles <10 nm, and highest (0.5) for particles 70-165 nm. PRACTICAL IMPLICATIONS This study provides insight into the penetration of UFP into homes and the resulting change in particle size distributions as particles move indoors near a major diesel traffic source. Although people spend most of their time in their homes, exposure estimates for epidemiological studies are generally determined using ambient concentrations. The findings of this study will contribute to improved size-resolved UFP exposure estimates for near roadway exposure assessments and epidemiological studies.
Climate change, global food supply and risk of hunger

This paper reports the results of a series of research projects which have aimed to evaluate the implications of climate change for food production and risk of hunger. There are three sets of results: (a) for IS92a (previously described as a ‘business-as-usual’ climate scenario); (b) for stabilization scenarios at 550 and 750 ppm and (c) for Special Report on Emissions Scenarios (SRES). The main conclusions are: (i) the region of greatest risk is Africa; (ii) stabilization at 750 ppm avoids some but not most of the risk, while stabilization at 550 ppm avoids most of the risk and (iii) the impact of climate change on risk of hunger is influenced greatly by pathways of development. For example, a SRES B2 development pathway is characterized by much lower levels of risk than A2; and this is largely explained by differing levels of income and technology not by differing amounts of climate forcing.
Novel function of transcription factor ATF5: blockade of p53-dependent apoptosis induced by ionizing irradiation.

PURPOSE To find a new molecule that affects p53-dependent radiosensitivity. METHODS AND MATERIALS A mouse sarcoma cell line, QRsP(p53+/+), was used. From this cell line, we established a radiosensitive clone and a radioresistant one. Colony assay, p53 gene transfer, a luciferase assay for p53 and p21, animal transplantation experiment, and DNA array analyses were performed. RESULTS Microarray showed marked reduction of a transcription factor, ATF5, both in vitro and in vivo for the radiosensitive clone. Interestingly, flow cytometric analysis demonstrated marked apoptosis for the radiosensitive clone by p53 cloned adenovirus infection. Luciferase reporter assay revealed that ATF5 suppressed the transactivational activity of p53 and p63. By ATF5 gene transfer, the radiosensitive clone regained resistance to both ionizing-radiation and Ad-p53 infection-induced cell death. Surprisingly, time-lapse cell migration observation revealed greater cell motility for ATF5-transfected radiosensitive clone. CONCLUSIONS It seems likely that ATF5 is a potent repressor of p53 and elevated expression of ATF5 in a tumor may relate to enhanced malignant phenotypes, such as radioresistance or greater cell motility.
Selección de métricas de paisaje mediante análisis de componentes principales para la descripción de los cambios de uso y cobertura del suelo del Algarve, Portugal

Las herramientas propias de la Ecologia del Paisaje son un instrumento ampliamente utilizado en el analisis de la estructura de los paisajes para cuantificar sus caracteristicas espaciales y las relaciones con los procesos territoriales. Sin embargo, el amplio numero de metricas disponibles y el grado de correlacion existente entre ellas han generado la necesidad de seleccionar conjuntos reducidos, adecuados para su aplicacion en diferentes contextos y paisajes. Este articulo presenta la seleccion de un conjunto de estas metricas para una de las regiones mas dinamicas de Portugal, como el Algarve, mediante un analisis de componentes principales y empleando para ello la cartografia del proyecto Corine Land Cover para tres fechas CLC 1990 (imagenes de 1985), 2000 y 2006. Los resultados del analisis han permitido determinar tres componentes principales presentes en las tres fechas seleccionadas, asociados con el area de las teselas y la forma de aquellas de mayor tamano, y, en menor medida, con su elongacion/compacidad y con la complejidad de los bordes. A partir de estos componentes se ha podido seleccionar un conjunto reducido de seis metricas, empleado para caracterizar los principales cambios que han tenido lugar en la region durante ese periodo. De dichos cambios destacan, entre otros, los procesos de fragmentacion de los paisajes naturales del norte de la region, con las posibles implicaciones ecologicas derivadas.
Instructions for use Title Circadian PER 2 : : LUC rhythms in the olfactory bulb of freely moving mice depend on thesuprachiasmatic nucleus but not on behaviour rhythms

Right This is the peer reviewed version of the following article: Ono, D. , Honma, S. , Honma, K. -i. (2015), Circadian PER2::LUC rhythms in the olfactory bulb of freely moving mice depend on the suprachiasmatic nucleus but not on behaviour rhythms. European Journal of Neuroscience, 42: 3128‒3137. , which has been published in final form at http://doi.org/10.1111/ejn.13111. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for SelfArchiving. Type article (author version)
STUDIES ON THE PHYSIOLOGY OF THE PARATHYROID GLANDS: II. The Relation of the Serum Calcium to the Serum Phosphorus at Different Levels of Parathyroid Activity.

In 1922 Howland and Kramer (1) emphasized the fact that the deposition of calcium in the bones depends not alone on the calcium level in the blood, nor alone on the phosphorus level in the blood, but on the levels of both calcium and phosphorus. They refrained from any long physical-chemical discussion of ion products, etc., but contented themselves with multiplying the serum calcium in milligrams by the serum phosphorus in milligrams and arriving at the very useful clinical fact that, when this product was below 30 in young children or young rats, active rickets was present. When the product was above 40, rickets was either absent or healing had set in. Shipley, Kramer, and Howland (2) showed that when the product of Ca X P is 35 calcification is obtained in vitro, not only when the calcium is 10 mgm. , and the phosphorus is 3.5 mgm. , but also when the calcium is 5 mgm. , and the phosphorus is 7 mgm. The original clinical observation of Howland and Kramer has stimulated many attempts from the laboratory to elucidate the physical-chemical laws which govern the calcium and phosphorus levels in the blood and the precipitation of these elements in the bone. Although much progress has been made, the subject is not as yet entirely clear. We believe that a return to the analysis of clinical data may throw further light on this subject. In this paper an analysis is made of the inter-relation of serum calcium
Therapeutic Hypothermia for Cardiac Arrest

In the era before Therapeutic Hypothermia (TH) was recommended and used as a therapeutic modality for out-of-hospital cardiac arrest (OHCA) patients, reported data suggests in-hospital mortality exceeded 58%. [1,2,3,4,5,6] Mortality after a sudden and unexpected cardiac arrest (CA) is high, and the chance of survival to hospital discharge has, until recently, remained unchanged. [7] In one report, OHCA in the U.S. has a mortality rate greater than 90% which results in more than 300,000 deaths per year. [8] Those who survive the devastating event, often retain a hypoxic brain injury and a permanently incapacitating neurologic deficit. [9] Studies of patients who survived to ICU admission but subsequently died in the hospital, brain injury was the cause of death in 68% after out-of-hospital cardiac arrest and in 23% after in-hospital cardiac arrest. [10,11] Therapeutic hypothermia, or targeted temperature management, is a therapeutic intervention that is intended to limit neurologic injury after a patient’s resuscitation from cardiac arrest.
Geometric transform invariant Brain-MR image analysis for tumor detection

In this work we propose a translational, rotational and scaling invariant scheme for possible detection of tumors in Brain-Magnetic Resonance (MR) images. The method incorporates the features like shape, position and texture to accurately diagnose from the infected images. The geometric transformation invariant nature of the method helps in detecting the tumor in various scales, positions and orientations, at a better rate compared to the state-of-the art methods. The method combines three features (shape, position and texture) to form a feature vector, which is used for detecting the infected parts in the image. In order to improve the accuracy of detection process, we employ a preprocessing step to denoise and enhance the images. The result section details the analysis and results of the proposed method and highlights on the accuracy of the method to properly identify the tumor parts in an MR image.
Rank Aggregation for Pattern Classifier Selection in Remote Sensing Images

In the past few years, segmentation and classification techniques have become a cornerstone of many successful remote sensing algorithms aiming at delineating geographic target objects. One common strategy relies on using multiple complex features to guide the delineation process with the objective of gathering complementary information for improving classification results. However, a persistent problem in this approach is how to combine different and noncorrelated feature descriptors automatically. In this regard, one solution is to combine them through multiple classifier systems (MCSs) in which the diversity of simple/noncomplex classifiers is an essential issue in the definition of appropriate strategies for classifier fusion. In this paper, we propose a novel strategy for selecting classifiers (whereby a classifier is taken as a pair of learning method plus image descriptor) to be combined in MCS. In the proposed solution, diversity measures are used to assess the degree of agreement/disagreement between pairs of classifiers and ranked lists are created to sort them according to their diversity score. Thereafter, the classifiers are also sorted according to their performance through different evaluation measures (e.g., kappa and tau indices). In the end, a rank aggregation method is proposed to select the most suitable classifiers based on both the diversity and the effectiveness performance of classifiers. The proposed fusion framework has targeted at coffee crop classification and urban recognition but it is general enough to be used in a variety of other pattern recognition problems. Experimental results demonstrate that the novel strategy yields good results when compared to several baselines while using fewer classifiers and being much more efficient.
The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants

In pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI), plant cell surface receptors sense potential microbial pathogens by recognizing elicitors called PAMPs. Although diverse PAMPs trigger PTI through distinct receptors, the resulting intracellular responses overlap extensively. Despite this, a common component(s) linking signal perception with transduction remains unknown. In this study, we identify SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK)3/brassinosteroid-associated kinase (BAK)1, a receptor-like kinase previously implicated in hormone signaling, as a component of plant PTI. In Arabidopsis thaliana, AtSERK3/BAK1 rapidly enters an elicitor-dependent complex with FLAGELLIN SENSING 2 (FLS2), the receptor for the bacterial PAMP flagellin and its peptide derivative flg22. In the absence of AtSERK3/BAK1, early flg22-dependent responses are greatly reduced in both A. thaliana and Nicotiana benthamiana. Furthermore, N. benthamiana Serk3/Bak1 is required for full responses to unrelated PAMPs and, importantly, for restriction of bacterial and oomycete infections. Thus, SERK3/BAK1 appears to integrate diverse perception events into downstream PAMP responses, leading to immunity against a range of invading microbes.
A ARTE DE FIDELIZAR CLIENTES COMO DIFERENCIAL COMPETITIVO

Este trabalho pretende avaliar as praticas de fidelizacao de clientes adotadas pela empresa Moveis Conquista, a luz da literatura especializada. A metodologia considera duas vertentes, uma marcada pela revisao de literatura e outra na analise de uma empresa, considerada como estudo de caso, de cunho exploratorio-avaliativo. Tomou-se por base a comparacao entre os indicativos das fontes teoricas consultadas e a pratica da referida empresa. Para o levantamento de dados, foram realizadas entrevistas semiestruturadas com os gestores e colaboradores. A partir da comparacao, identificou-se a necessidade da empresa Moveis Conquista adotar estrategias competitivas, voltadas para a manutencao do cliente, com medidas capazes de desenvolver um relacionamento ainda maior. Identificou-se ainda que, diante do novo cenario de negocios, a referida empresa, a partir das acoes em curso, vem ganhando cada vez mais espaco no mercado, reconhecendo que os clientes devem ser considerados necessarios para o sucesso da organizacao, assim como reconhece a necessidade de aperfeicoar as ferramentas de relacionamento ja utilizadas.
Andrographis paniculata, the Key Facts for Therapeutic Use

Andrographis paniculata Nees is a slender upright annual varying in height from 30 to 100 cm (1 to 3 feet), with a square stem and « lanceolate leaves » (i.e., shaped like a lance, sharp at the ends and curved in the middle). Andrographis is indigenous to the plains of India where it is called Kalmegh (in the Bengali language) or Kiryat (in the Hindi language). It is cultivated throughout Southeast Asia and in China where it is called Chuan Xin Lian, and has also been introduced to the West Indies.
Adaptive Random Access with Beam-forming in 4G Mobile Networks

Packet switching replaces the traditional circuit switching and with the wireless extension of the Internet next-generation air-interfaces have to cope with bursty IP traffic. Transmit resources on the air-interface have to be allocated on demand and in a very flexible manner. At the same time, the delay requirements are more stringent putting high challenges on the random access component of future wireless systems. In this paper a novel random access scheme exploiting the advantages of multiple-input multiple-output (MIMO) systems is presented that considerably enhances the spectral efficiency. Besides a heuristic algorithm for beam-forming the advantages are described and the performance gains are derived. Since MIMO will be part of 4G mobile networks and random access is a basic component in every wireless system the new approach is a promising candidate for systems beyond 3G
Crystal structural analysis and metal‐dependent stability and activity studies of the ColE7 endonuclease domain in complex with DNA/Zn2+ or inhibitor/Ni2+

The nuclease domain of ColE7 (N‐ColE7) contains an H‐N‐H motif that folds in a ββα‐metal topology. Here we report the crystal structures of a Zn2+‐bound N‐ColE7 (H545E mutant) in complex with a 12‐bp duplex DNA and a Ni2+‐bound N‐ColE7 in complex with the inhibitor Im7 at a resolution of 2.5 Å and 2.0 Å, respectively. Metal‐dependent cleavage assays showed that N‐ColE7 cleaves double‐stranded DNA with a single metal ion cofactor, Ni2+, Mg2+, Mn2+, and Zn2+. ColE7 purified from Escherichia coli contains an endogenous zinc ion that was not replaced by Mg2+ atconcentrations of <25mM, indicating thatzincisthe physiologically relevant metal ion in N‐ColE7 in host E. coli. In the crystal structure of N‐ColE7/DNA complex, the zinc ion is directly coordinated to three histidines and the DNA scissile phosphate in a tetrahedral geometry. In contrast, Ni2+ is bound in N‐ColE7 in two different modes, to four ligands (three histidines and one phosphate ion), or to five ligands with an additional water molecule. These data suggest that the divalent metal ion in the His‐metal finger motif can be coordinated to six ligands, such as Mg2+ in I‐PpoI, Serratia nuclease and Vvn, five ligands or four ligands, such as Ni2+ or Zn2+ in ColE7. Universally, the metal ion in the His‐metal finger motif is bound to the DNA scissile phosphate and serves three roles during hydrolysis: polarization of the P–O bond for nucleophilic attack, stabilization of the phosphoanion transition state and stabilization of the cleaved product.
A biology-inspired model for the automatic dissemination of information in P2P networks

The Personal Intelligent Agents Framework (PIAF) is a framework developped in the context of research on smart web browsers. Compared to a classical browser, this smart browser should help the user to find and share information using the Internet network. This paper presents algorithms used in PIAF for the selective dissemination of information in a P2P network. They use ideas from epidemic systems and are based on an artificial ant colony model. The main contribution made to the domain of selective information diffusion is the use of estimated profiles defined by pheromone trails associated to connections between peers.
At High Levels, Constitutively Activated STAT3 Induces Apoptosis of Chronic Lymphocytic Leukemia Cells

In chronic lymphocytic leukemia (CLL), the increment in PBLs is slower than the expected increment calculated from the cells’ proliferation rate, suggesting that cellular proliferation and apoptosis are concurrent. Exploring this phenomenon, we found overexpression of caspase-3, higher cleaved poly (ADP-ribose) polymerase levels (p < 0.007), and a higher apoptosis rate in cells from patients with high counts compared with cells from patients with low counts. Although we previously found that STAT3 protects CLL cells from apoptosis, STAT3 levels were significantly higher in cells from patients with high counts than in cells from patients with low counts. Furthermore, overexpression of STAT3 did not protect the cells. Rather, it upregulated caspase-3 and induced apoptosis. Remarkably, putative STAT3 binding sites were identified in the caspase-3 promoter, and a luciferase assay, chromatin immunoprecipitation, and an EMSA revealed that STAT3 activated caspase-3. However, caspase-3 levels increased only when STAT3 levels were sufficiently high. Using chromatin immunoprecipitation and EMSA, we found that STAT3 binds with low affinity to the caspase-3 promoter, suggesting that at high levels, STAT3 activates proapoptotic mechanisms and induces apoptosis in CLL cells.